LOCUS       DAOSAL010000001       113808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_119_length_113808_cov_51.580820, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000001 DAOSAL010000000
VERSION     DAOSAL010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 113808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 113808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..113808
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119_length_113808_cov_51.580820"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            232..591
                     /locus_tag="PK049_00005"
     CDS             232..591
                     /locus_tag="PK049_00005"
                     /inference="COORDINATES: protein motif:HMM:NF039670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6150 family protein"
                     /protein_id="HOK59970.1"
                     /translation="MRLSIKILSILFVLLGFSGISAAQKFYEAEDVKDAQVKFFIVDS
                     PEQADLHFCIIYEASEITKPGVMMEVETPKEADITLIFVDNEKDADIKVWLVDTPAEV
                     KWINESKKKLLSVGGLK"
     gene            661..1830
                     /locus_tag="PK049_00010"
     CDS             661..1830
                     /locus_tag="PK049_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOK59971.1"
                     /translation="MNKYSRYDQRGVSASKDDVHKAISNLDKGLFPKAFCKVVPDYLT
                     GDPDYCLVMHADGAGTKSSLAYVYWRETGDMSVWKGIAQDAIVMNIDDLLCVGITDNI
                     LLSSTIGRNKNLIPGEVVSAIIDGTEEFCQTMRSMGINIVLTGGETADVGDLVRTVIV
                     DSTVTARIKRSQVISNSNIQDGDVIIGLASFGQASYEKEYNGGMGSNGLTSARHDVFG
                     HYLAQKYPESYDPQVPESLVYSGNLKLEQPVEGTGLTAGKLVLSPTRTYAPIIKAILD
                     NYRPVIHGMVHCSGGAQTKVMNFVNNLHVVKDNLFPVPPLFRIIQEQSNTPWQEMYKV
                     FNMGHRFEIYVYPEFADDIIAIAKEFKVDAQIVGYCTEATSNKLTIKSPYGEFHY"
     gene            1878..2969
                     /gene="prfA"
                     /locus_tag="PK049_00015"
     CDS             1878..2969
                     /gene="prfA"
                     /locus_tag="PK049_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOK59972.1"
                     /translation="MADNTILRKLEGIRHKFEEIGQQITDPAVMADMKKFVALNKEYR
                     QLEPIVEAYEKYKNVLSNIESSRQILANEKDEELREMAKMELETLTQEQEQLEEEIKI
                     LLLPADPQDSKNAVMEIRAGTGGDEASIFAGDLFRMYTKFFEKKGWKYEVTSFSEGTV
                     GGYKEIIMNVTGNGVYGVLKYESGVHRVQRVPQTETQGRVHTSAATVAVLPEADEFDI
                     DLKMEDIRKDTFCSSGPGGQSVNTTYSAIRLTHIPTGIVVQCQDQKSQIKNFEKALTE
                     LRTRLYNMEYQKYLDEIASKRKTMVSTGDRSAKIRTYNYPQGRVTDHRINLTLYNLSA
                     IMDGDLQEVIDKLQLAENAERLKAAAAEE"
     gene            2982..3815
                     /gene="pyrF"
                     /locus_tag="PK049_00020"
     CDS             2982..3815
                     /gene="pyrF"
                     /locus_tag="PK049_00020"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOK59973.1"
                     /translation="MTAQELFEQIKKKRSFLCVGLDTELTKIPEHLLTKDDPMFEFNR
                     KIIEATHDLAIAYKLNLAFYEVHGAAGWNSLVKTVKYLKTHHPDVLVIADAKRGDIAN
                     TARMYAKAYFHSLGCDAVTVSPYLGQDSVVPYLQYDGKWTILLAITSNESFGDFQLVH
                     NKQTGNLLFEDVVLKSMHWGSDANMMFVVGATHPEYFAAVRKHAPHHFLLVPGVGEQG
                     GSLQKVAENGMNDRCGLIVNSSRGIIYADITGNFTSEARKRALKLQQEMEVLLKQNGL
                     I"
     gene            complement(3822..4937)
                     /locus_tag="PK049_00025"
     CDS             complement(3822..4937)
                     /locus_tag="PK049_00025"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HOK59974.1"
                     /translation="MKNRWKVLLITTGVLLGILSCSRDSGNIVIGIDDIGKHDRVLIL
                     VHPTENNIKTFNYLIDSKILPIPSVTKVIGVYHTSEKYDYSKSVEYIKKNKLENFKLL
                     PVKEPITETAIYRVNECTDVFARLTKVSDGVIFTGGPDIPPSIYGEKTSLLTQITDPA
                     RHYFEVSFMFHLIGGSKDTLFQPLLDAKPLMPVLGICLGMQTMNVAAGGTLVQDIPSE
                     IYGIESVEDALIQQTNSLHRNYNANYALDDKLLWGNFHQISILDEPLKSICQGQPWVL
                     SSHHQGVERLGRNLKIIATSIDGNVVEAIVHTKYPNVIGLQFHPEPTLLYNNDEFLRF
                     LPNQPSRLTYLNMYSGEAGEKFHLNFWSWFGKKVIEQ"
     gene            complement(4956..6413)
                     /locus_tag="PK049_00030"
     CDS             complement(4956..6413)
                     /locus_tag="PK049_00030"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOK59975.1"
                     /translation="MNRITIIASALATFYCSVLSFAQQVDSNMLKDHVYYLASDSLMG
                     RGFGTEGGRMAAKYITDKFKEVGLTPWQGQYLHSFISSSMMLKTEGANIIGWVEGNDP
                     VLKNEYIVLGAHYDHVAYKIVNGEKVVYNGADDNASGVASIIEIGRWLVLNRDKLKRS
                     VIIIAFDGEEAGLIGSSHLVNNKVIPTDRVKFMFSLDMVGMLQKYGGIDLVGNKTLSD
                     GDEFFVQLASKHGIQVKKSGRSVEMQTDTQPFGKIGIPAVHVFTSTVSPYHKPEDDAD
                     KLDYVGMAKIASFVADATLQLSTTDSVKPDARFAAKTLGSDKFSAGYQVGIGNTYHDY
                     RDEFYNSKEGFSFNTGLSFTIGLSRYIDLQVGALYQTTGTEHRYGHFRTHEVMAPASI
                     LLYMGGKSKQMLSPNLYFIGGGYYSYRFAGKVGNGSFDMDNNIQKDNYGFQVGVGFSI
                     MNVNFQFVRQVAVLSLEKDRNVFPSTLMFNMGFRF"
     gene            6694..7290
                     /locus_tag="PK049_00035"
     CDS             6694..7290
                     /locus_tag="PK049_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59976.1"
                     /translation="MKKTLLLGFAALTMVAMLSSCGKVPQEKIDAANAAIDSAKAAEA
                     DVYLAADFAAVQDSMSAVMADIEVQKSKLFKKFGPASEKLDRILGMAQQLKVNAVAKK
                     EEVKKEVEGLMTEVATMMEENGKLMKKAPRGKEGAAVLEQMKAEMATIEATVNEAKGL
                     YDAGKYMDASNKMKAAKESATKINTELNEAIAKVKGRK"
     gene            7360..8502
                     /locus_tag="PK049_00040"
     CDS             7360..8502
                     /locus_tag="PK049_00040"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOK59977.1"
                     /translation="MKPILKYSLITLATLSVIGGGFALFYYSIPEPPKGDIDYALASL
                     HQASRSNAKRYSKQKYLKAKAYFDSAMKHWNIQNERFILSRDYQKVSYFAKKSAEMAK
                     SATSNSKELSKNLVISVKDKIDNLTALVSNMNNLFQRLPLPDEFRKKISKGKMLIHEA
                     EVAFKKGDYLESSEKIKLAEDLIMDSYKKTLDDLEDYFNSQPYWNRLVKSAISNTAKN
                     NSMAIIVDKFARKLYIYQNGTKKYEFDAEFGKNWMGRKRLKGDKATPEGIYLVKSKIP
                     ANKTKYFRALLLNYPNSEDIENFNKEKASGRIPKHAQIGGLIEIHGDGGKGTDWTDGC
                     IALDNNDMLQVYKIVEIGTPVVIVGSIADFNTIAMSLNLKRNQIKG"
     gene            8505..9221
                     /locus_tag="PK049_00045"
     CDS             8505..9221
                     /locus_tag="PK049_00045"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOK59978.1"
                     /translation="MNSNEDKIDFNPEINPKLKRLGFVMLLINIILSGFIILYFYLTY
                     LSQPMAELVVKNRKLTFVSDSIQDDKVDVKKLQKEVASLKQQFQNLTPAQPYIVVNTT
                     YNRFRLYKNRKQVREGFCSTGSYTLLKSHDKRQWIFHTPKGMYKVLGKVTNPVWRRPD
                     WSYVEEGLPIPSANDPSRWEAGVLGDYALSIGDGYLIHGTIYKRFLGMPVTHGCIRMN
                     DEDLEAVYKTLDLGSKVYIY"
     gene            9233..10018
                     /locus_tag="PK049_00050"
     CDS             9233..10018
                     /locus_tag="PK049_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59979.1"
                     /translation="MRKPIKITLYTTAILVVILAVAYTILKVIAFQKAINNIEQHIAK
                     ELNKRKKTVEWVSEPDSTLKKLLVDKAYYNALITASKTDSIFLAINIPDSTVSLMIKG
                     VVAHKAKAEHIKVSPLFSAINPPLLIQGLSVPLTVSKHTSTIPKVPLMIKIAPRDTSE
                     YTPDVIPDTTDLDPVNFIIELKPNILLYFYESNPSQSSDGFNQFKFDLRERTWTVVQD
                     VKRMLKLKTPVYKPYIRFKLGKKDAKVIYRAIPNQGQVVLRLE"
     gene            complement(10050..10123)
                     /locus_tag="PK049_00055"
     tRNA            complement(10050..10123)
                     /locus_tag="PK049_00055"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10087..10089),aa:Pro,seq:cgg)
     gene            10229..11500
                     /locus_tag="PK049_00060"
     CDS             10229..11500
                     /locus_tag="PK049_00060"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HOK59980.1"
                     /translation="MDESLLHFQWRYQHYTLNGLSTTKGDTVEVIDPGLYNTDSGPDF
                     SNAKVRIGDTLWVGNVEMHIKSSDWFTHNHQTDNAYNNVILHVVVEHDRTAVTSKGTE
                     VQTLVIPYPNKLVHSHENLIKSTEWIPCASKIGVVDSLLINSTIDRMQVERLENKSKR
                     VAELVSQCNGSWEEAFYRDLCRSFGLKSNALPFQLLAQSLPQKVLAKHKNSAFQLEAL
                     LFGQSGLLDKAKNDSYVISLKQEYTYLKNKFSLTPIEPSLWKFMRLRPASFPTIRIAL
                     LAMLAHKSSGLFSQLMEVKNIDNVIKFFAVEASDYWTNHYVFGEESISKKKRLGEETI
                     RLITINTIIPFMYSYGRSRAKADLEANALTILQQMPPENNTIVRNFGALGIKALSAFD
                     SQALVHLKTFYCETRKCIYCPIGVSILRKQL"
     gene            11527..12531
                     /locus_tag="PK049_00065"
     CDS             11527..12531
                     /locus_tag="PK049_00065"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel protein"
                     /protein_id="HOK59981.1"
                     /translation="MEQKGGRTLALAITILLSIITIGTVGYIHIEDFTFTEAIYMTVI
                     TIGTVGFKEVRPLSPEGMWFTITLIIISIGFFAYAATFLAKYFSEQILSRYSNKKIME
                     KMIAKMQNHVIVVGYGRNGSQAVEELLAHNIPVVVIENRPKVIEYIQQNPKIAYVDGD
                     ATLDITLQKAGVDKAKALITALANDADNLFVVITAREMNPKLKIISRASAVTNDRKLK
                     LAGADNVILPDKVGGQKMAKLVIQPDILEFVENIMLKRTGNISLFEISCRKILEVGAK
                     SLDELQIREKTGVNVIGVRNPIGEYILNPEPNHILTHDSLLFVLGTTEQVEKLKDLIS
                     "
     gene            12543..13430
                     /locus_tag="PK049_00070"
     CDS             12543..13430
                     /locus_tag="PK049_00070"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOK59982.1"
                     /translation="MKILVTGANGLVGKNIAKALHATNQVEVFATSLKKFQLEGVTAF
                     TSNLLNADINHMVDDIKPEIVVHCAALSSPDACEVDRYACHRMNVELTKRIVSACVDY
                     NVHLVFLSTDFIFDGSKGNYTEMDAPNPVNYYGESKLEAEKLIQASGVVNTIVRTSLV
                     YGVESKLSRPNIITRVFDNLSKNKQYKVPSDQIRTPTYAPDLASAISQICLNRVTGIY
                     NISGDQIISVADFAILAAKAFDLNHELLLQVNSKDLAEAAPRPKNTSLVIGKAKNDLN
                     FKPTPIEMALGKIKTTLNS"
     gene            13593..13865
                     /gene="rpsO"
                     /locus_tag="PK049_00075"
     CDS             13593..13865
                     /gene="rpsO"
                     /locus_tag="PK049_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOK59983.1"
                     /translation="MNYLTAEQKKEIFEKYGKSNTDTGSPESQIALFSYRINHLTEHL
                     KVHRKDYGTQRALLKLVGKRRRLLDYLKENDIERYRTIVKELNLRK"
     gene            14005..16131
                     /gene="pnp"
                     /locus_tag="PK049_00080"
     CDS             14005..16131
                     /gene="pnp"
                     /locus_tag="PK049_00080"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005824386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOK59984.1"
                     /translation="MQKVVKKTIDLGDGRTITIETGKLAKQAHGSVVLTMGKTMLLAT
                     VVSAQEAKEDVDFMPLSVEYKEKFAASGRFPGGFLKRESKPSDYEILVARLVDRALRP
                     LFPDDYHAETFVTVNLISAEKEIMPDALAGLAASAALAVSDVPFHGPISEVRVARING
                     KLKINPTFKEVEEADIDMIVGATYDNILMVEGELKEVSEADMLEAIKFAHEAIKPQCL
                     AQMELAKELGVVKRTYCHETNDEELKAKVHEFCYDKVYQVAKSMLPKQERTAAFEAIK
                     QAFIETIPEEEREAKQFMVNRYYHAVEKEAMRNMILNEGVRLDGRKTHEIRPIWCEVD
                     YLPMAHGSAIFTRGETQSLTTVTLGTKLDEKIIDEVLMQGTEKFTLHYNFPPFSTGEA
                     KPSRGVSRREIGHGNLAHRALKNMVPDAPENPYAIRVVSDILESNGSSSMATVCAGTL
                     ALMDAGVQIKKPVSGIAMGLITEKGSDRYAVLSDILGDEDHLGDMDFKVAGTRDGITA
                     TQMDIKVDGLSYEVLAKALKQAHDGRMHILDIMMQTISKPRPDFKPHAPRIEQFNIPR
                     ETIGAVIGPGGKVIQEIQRETQTTITIEEINDHGVVSVFAESAESRDAAVKWIKGIVA
                     VPEVGEIYKGKVKSIMPFGAFVEILPGKDGLLHISEIEWRRLEKVEEVLKEGDMVDVK
                     LIEIDKKTGKLKLSRKVLLPKPEKNK"
     gene            16262..18376
                     /locus_tag="PK049_00085"
     CDS             16262..18376
                     /locus_tag="PK049_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007214917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOK59985.1"
                     /translation="MKKIFILMLLATPILITMSCNTAKVKYPKTKQVDVVDEYFGVKV
                     ADPYRWLEDDKAPEVAEWVKQQNEVTFNYLNAIPFRNKIKERLTTIWNYTSMGAPFKA
                     GGRYFFFKKEGLQNQSVLYMMENLEAEPKVILDPNTFSEDGTVSLSNVSVSRDGKYLA
                     YSINDGGSDWQKIKVIKLPGGEQLPDEISWVKFSSIAWLNDGFFYSRYDEPKGGSELS
                     NINQNHKVFYHKVGTPVETDKLIFSNSQYPLRNYSAQTTDDEKYLIIYESESTYGNSI
                     YVKNLKDPRANFSKITTGFEFEYSVLDHVDDNLIVLTNYKAPKYKLVKINVNSMDIGN
                     WRDILPERKDVLKECALINKKIIANYIVDAKSQAEVYNLDGSKITDIELPTLGTISGI
                     SGDINDSIAFYSFSSFTVPPTVVKYNVNTNTSTQFFSPNIDFKFDDYETKQVFYTSKD
                     GTLVPMFIVHKKGIKLDGNNPTLLYGYGGFNVSLLPYFSTSRLFWLEQGGIFAMANLR
                     GGGEYGENWYRAGTKLNKQNVFDDFIAAAEYLIKEKYTSPKKLAIQGGSNGGLLIGAV
                     TNQRPDLFKVAIPQVGVMDMLRYHNFTIGWAWANDYGTSNDSIQFLNLYSYSPLHNIK
                     PKTNYPAILVTTADHDDRVVPAHSFKYIATMQEKNPNPKNPALIRIQTRAGHGAGMPT
                     SMVIDEYADIYAFIMYNMGMQY"
     gene            18480..19109
                     /locus_tag="PK049_00090"
     CDS             18480..19109
                     /locus_tag="PK049_00090"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HOK59986.1"
                     /translation="MHYLVIEGNIGAGKTTLATMLAKELNAKLILEQFADNPFLPKFY
                     KNPERYSFPLELSFLAERYSQLNNELRSASLFHSLTIADYYFMKSLIFAQNTLSGDEL
                     QLYKQLFSIIYSTLPKPDLYVYLHLPTNQLLKNIRKRGRDYEQTITAEYLNGITEGYF
                     DFFKQHPEYTFLVIDTSKIDFVANRDDFLKIKSTIFDQHYKKGVNLVSL"
     gene            19226..20926
                     /locus_tag="PK049_00095"
     CDS             19226..20926
                     /locus_tag="PK049_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59987.1"
                     /translation="MKMKGLKLSIIALAGLLLASCGGVEKMKELPAGFTLKVTPNPVE
                     VHANKVKATFEGTIPPKYFNKKATVEITPVVVYKGGELALPSKVLQGEDVQANNQVIN
                     YEAGGSWKHDVEFDYKDEMFSSEIELRFVITYKDKKVPFDAPQKVGIGTMATYKLVDI
                     DVKPVMLNDKFVRVTPEEKEAQINFVIQKADIRKSELTKDEIKMLNDFLLAAAQAKNK
                     EIKELKIAAYASPDGPEKLNDKLSQDRGKNTDKYLADVFKKAKVQGVTPAVEFTAEDW
                     DGFQKAVQASDIEDKELIIRVLSMYSDPVVREKEIKNLSKVYKVLAEKILPQLRRSRM
                     VAKVDVIGYSDDTLKYMVENNAYENLNVEEMLYAATLVNDVNKKIEVYMKAGEKFNCA
                     RGYNNAAYYYILNKDVANAKVALDKIPADQKIAAVTNNRGCVAMLEGKFDEAEKLFAD
                     AADAGSAVKYNLGIIAILKGQYPAAAEYLAGSDSFNQGLALLLAGKVDAAKNTFNKVE
                     TAKGYYGRAIVGARLAEEATVLDNLRTAFGKDASLKDRAKEDVEFRTFFQNEAFKALF
                     "
     gene            complement(21035..21577)
                     /locus_tag="PK049_00100"
     CDS             complement(21035..21577)
                     /locus_tag="PK049_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009121979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOK59988.1"
                     /translation="MVKNRKLLNEELGRKSIDEFKSSEKLPIAVVLDNVRSLNNVGSV
                     FRTSDAFLIEKIYLCGITGIPPHPEIHRSALGAENSVDWEYADSTVKAVEGLKQSGYV
                     IISVEQVEHSVLLNQFYPKKGQKYALVFGNEVKGVGQDVVDISDVCIEIPQLGTKHSF
                     NISVSAGIVLWDFFVKVMVD"
     gene            21653..22840
                     /gene="sucC"
                     /locus_tag="PK049_00105"
     CDS             21653..22840
                     /gene="sucC"
                     /locus_tag="PK049_00105"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HOK59989.1"
                     /translation="MNLHEYQSKEILRQAGVPVPVGLVAFSKDEAIRAAQEVMKLTGT
                     NTIAVKAQIHAGGRGKGGGVKIAKSIDEVSTYASNILGMNLVTHQTGPQGKLVRKVLI
                     EQGIYQPGPSEIKEYYVSILLNRASGRHMIIYSPQGGMDIEAVAAQTPELIFKEEIHP
                     VGLQAFQARKIAFNLGLSGKAYQQMVKFVLSLYEAYTKNDLSLLEINPVVKTSNDDIL
                     AADAKCSIDDNALFRHPDLAEYRDIYEEDPAEVEAGEHNLNFVKLDGNVGCMVNGAGL
                     AMATMDIIKLSGGDPANFLDVGGGANAKTVAAGFRIILKDPNVKAILINIFGGIVRCD
                     RVAQGVVDAYKEIGNIPVPVIVRLQGTNALEGKKLIDESGLKVYSAITLQEAANLVKQ
                     MVN"
     assembly_gap    22881..22980
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(23027..23215)
                     /locus_tag="PK049_00110"
     CDS             complement(23027..23215)
                     /locus_tag="PK049_00110"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HOK59990.1"
                     /translation="MEKNVGKIDKVIRILIALVITGVGIYYQSWWGLLAIVPLATALT
                     GTCGLYSLFGISTCSTKK"
     gene            complement(23272..24534)
                     /locus_tag="PK049_00115"
     CDS             complement(23272..24534)
                     /locus_tag="PK049_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HOK59991.1"
                     /translation="MERVNVYLNSEIGELEGVILHTPGAEVENMTPENAQRALYSDIL
                     NLNVARKEYSQLSGVLSKVAKTYEVVDLLEKVLKNEENREYLLEKICRHENAMSLFDY
                     LLDIDAKQLARMLIEGVPLVRNTLTNFLSKERFALRPLYNFYFTRDASIAIGEDVLIA
                     KMANAVRDRESIIMEAIFSRSGIFNTQTINPTTFSNPADVTIEGGDVLVVRDDILLIG
                     NGIRTNTHGIDFILSRFLARDDKRKRHILVQELPYKPESFIHLDMVFTMLDFDKCMVY
                     EPIILQPNRYQTVHITIDKGKVKEIKTVDNLLVALKSLGIDLKPIVCGGTKDRWIQER
                     EQWHSGANFVAIGPGRVIGYGRNINTLEEMNKNGFEILRAKDIVAGKVDMNSYQKYVV
                     ALDGSELPRGGGGARCMTMPFRRKPIKW"
     gene            complement(24736..27603)
                     /gene="gcvP"
                     /locus_tag="PK049_00120"
     CDS             complement(24736..27603)
                     /gene="gcvP"
                     /locus_tag="PK049_00120"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HOK59992.1"
                     /translation="MYTDKFVSRHNGPSDHEVGEMLKVIGVSSVDELINQTIPANIRL
                     KKPLNLPAPMSEYEFLTHMHKVASKNKLYKSFIGMGYYGTVTPSVILRNIFENPGWYT
                     SYTPYQAEISQGRLEALLNFQTMVVELTGMQIANASLLDEATAAAEAMLMMLNLRSRE
                     AVKAGKNVIFVDQDIFPQSLDVIKTRSNPQGIEVVTGCYSEYNFTGREFGVIVQYPSA
                     KGNIRDYAAFAAKAHEAGALVTAVSDILSLALITPPGEWGADIACGSTQRFGIPMGFG
                     GPHAAYFACKDEFKRSMPGRIIGVSIDRHGNKALRMALQTREQHIKRERATSNICTAQ
                     ALLATMAGMYAVYHGPEGIRRIASYVHSTAVQVAKELVGMGYKQNVKNIFDTLEVELP
                     SGVTIDDIRKRALAREINLNYKPNGTVGISFDETTSVTDVNNLLSVFAESAGKKFNSI
                     AEIKETIEIPENLRRKTPFLTDKVFNSFHSETEMMRYIKKLERRDIALNHSMISLGSC
                     TMKLNPATTMLPLSWPEWNSLHPFVPADQAEGYMQVIRELEQYLAEITGFHAVTLQPN
                     SGAAGEYTGLMVIRQYHINRGEGHRNVVLIPSSAHGTNPASAAMAGMQVVVVACDEKG
                     NINVDDLRAKAEQHKQNLAAFMVTYPSTHGVFESKIREMMDIVHQNGGLVYMDGANMN
                     AQVGLTSPGEIGADVCHLNLHKTFAIPHGGGGPGVGPIAVAKHLAPFLPSHPVVKTGG
                     EKAITAVAAAPWGSASIIVISYAYILMLGAEGLTQATKMAILNANYLAARLKDHYKIL
                     YTGETGRVAHEMILDCHHFKMAYGVDTSDVGRRMMDYGYHAPTVSFPVHESLMVEPTE
                     SESKQELDRFIDMMIAIKGELEDIKNGKADKADNLIANAPHTAPEVTADEWKHAYSRQ
                     QAAYPLPWVMQNKFWPYVSKIDAGYGDRNLVCTCAPIEDYM"
     gene            complement(27705..28340)
                     /locus_tag="PK049_00125"
     CDS             complement(27705..28340)
                     /locus_tag="PK049_00125"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOK59993.1"
                     /translation="MIAIKQFVFNPFQENTYVLYQPQGEAIVVDAGCSTESEFQELLD
                     FVHSQNLTVKCLINTHCHIDHILGIDKLKKQFGVKAFAHIDDFPLLQMAPAHAMMFWL
                     YLETVPAIDETLSHGDMIELNGDSIKVIHTPGHSRGGLCFYAEQERFILTGDTLFNLS
                     IGRTDLAGGNYETLIKSIREQLMVLPDDVIVYPGHGNSTTIGIERASNPFL"
     gene            complement(28348..29013)
                     /locus_tag="PK049_00130"
     CDS             complement(28348..29013)
                     /locus_tag="PK049_00130"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOK59994.1"
                     /translation="MILKNMLQDDSIRHKGLRRKLVEEVAAKGISDKRVLEAMLKVPR
                     HFFMDSGFLSFAYKDQAFPIGSGQTISQPFTVAFQTQLLQVSPMQKVLEVGTGSGYQA
                     AILMEMGARLYTIERHRELFLAAKALLEEMGYRPSCHYGDGYQGLPPYSPFDRIIVTA
                     GATEVPQALVDQLAPGGRLVIPVGNDYGQTMMLIEKLDDGTIKESAHGNFIFVPLLKG
                     KAK"
     gene            29149..29952
                     /locus_tag="PK049_00135"
     CDS             29149..29952
                     /locus_tag="PK049_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59995.1"
                     /translation="MTTCLVTDSNKLIPYQGENFFNNTIEANNQLIDKILLGICESNS
                     QIARLFNYPELLVVDTNPKTSYTIIANALRAGINVLTPSLPHFSSEKIQELITIAQEI
                     GVEFGFLPKYSLELPSTHSPIIIDSVKETINLTSTENLLQQIANDLIFLLSPIKSELR
                     KVRVYWLPLYSHPVSTLKLIVDFNDNSLITYQIKNKKETSMLKVVLTTEEYDKTFNIF
                     EAQIKPESTKETVMQNVDYFINQRKMLYPAAMALKSKQIIEIIIKKIYS"
     gene            29999..31405
                     /locus_tag="PK049_00140"
     CDS             29999..31405
                     /locus_tag="PK049_00140"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOK59996.1"
                     /translation="MNKKKQAFRYLLFDVAGSALAWTLFNLYRKRVIESNLFGIDIRF
                     NPDYKFYLGLVFFTLFWVSMFGLVGLYHDPYRKSRLREFGASLTITIIGTIVLFFVLL
                     IDDYVVAYQNYYKILLAMFLLVFALTYTPRLVITTQTIHRIHNRKIGYPTIIVGSGKK
                     AMQTYNDINSEKISQGYRIIGFIDLPNGVRVDQSLILGSVNNIPEVVARYGIEEVILA
                     IDNESPQTLTTVLNLLVPLNVNIKAIPSMYDILLGKVKLNRIIGTTLVDVSFEPMPAW
                     QANLKEMLDYFIAIVGLLLSAPVALILMAVIKTTSKGPVIFKQERIGQHGKPFHIYKF
                     RSMYVDAEANGPALSNQTDPRVTPVGRFMRKTRLDEIPNFINVLKGEMSIVGPRPERQ
                     FYIDQLVKVAPHYLHLQKVKPGITSWGQVKYGYAENIEQMVERLKYDLLYLENMSLYI
                     DFKIMIYTILTIFKGKGV"
     gene            complement(31402..31968)
                     /gene="thpR"
                     /locus_tag="PK049_00145"
     CDS             complement(31402..31968)
                     /gene="thpR"
                     /locus_tag="PK049_00145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOK59997.1"
                     /translation="METKRTFLAIDVNPQDNIINLLQELKRNHKLDSIKWVDPAIMHL
                     TIFFLGDTPSAQIPSICEMLKATIKKFEPFSIILKGIGYFGRPSPRVIWVGVDFPKLL
                     NELKLSVDKVLSEFDFSDDKEKFNPHLTLGRVKFMHNSDKLNAAIQQYRNKSFQVQEV
                     KELVLYESILKPQGPEYRAIQKFELKGC"
     gene            32044..32700
                     /gene="elbB"
                     /locus_tag="PK049_00150"
     CDS             32044..32700
                     /gene="elbB"
                     /locus_tag="PK049_00150"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008747.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HOK59998.1"
                     /translation="MGNNKKFAVILSGSGVFDGSEIHEAVMTLYAIAKHGATYEIFAP
                     NINQYHVINHIDGSVMNEKRNVLVESARIARGKIKPLNEFNPELFNALIIPGGFGVAK
                     NLSTWAFDGPKCSIHPQVEAAIKGMHKLKKPIGALCIAPVLLAKIIGNATLTIGKDTS
                     TAQAIESLGAKHQPTGHAQVVVDEKNMLFTTPCYMLDANIVDISNGAKAVVSEILKWI
                     "
     gene            32703..33164
                     /locus_tag="PK049_00155"
     CDS             32703..33164
                     /locus_tag="PK049_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK59999.1"
                     /translation="MKKLALLATIIATMFSYSCLNRCDVDCVNPPSPLWLKVVDKTTL
                     QNLIAEGFYHRDTIKIYYFDGVTKKYANIYYVGPVGQNDIIQTNIGILQPFEQLNTFY
                     LYLNQYDTDTILVRYVKSSDGCCTAYPLDSIAINGKYVDVDNVDYSFLIKK"
     gene            complement(33169..33771)
                     /locus_tag="PK049_00160"
     CDS             complement(33169..33771)
                     /locus_tag="PK049_00160"
                     /inference="COORDINATES: protein motif:HMM:NF025492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HOK60000.1"
                     /translation="MKRFSGTVLVAFLVAAAVKDVGAQAPSADSLILYPTIILDGEYV
                     PHIKIEDVVKVGKRRFKSRREMSQYYRMIYNLKKTYPYAQIAKYKLLEINENLKTLKT
                     DREKKEYIDKAEKELRNQFEKDLTKLTISQGKMLIKLIDRETGRTSYELVKELKGGFS
                     ATFWQGIARLFGSNLKTKFDPQGEDKILNELIFLYEQGLI"
     gene            34092..37130
                     /locus_tag="PK049_00165"
     CDS             34092..37130
                     /locus_tag="PK049_00165"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOK60001.1"
                     /translation="MKKIFGVISLILWCGSLLAQEINVSGTVTSSDGATLPGVTIVVK
                     ENPTKGAITDANGKYTISVLPNQTLEFSFVGMQTQTVPVGGRTQIDVVLQESTQMIEE
                     LIVVGYGVQKKSLVSSSVSKVSSEDIEKAAPTRVEQVLQGRTAGVQVIANSGQPGEGF
                     TVRIRGVGTNGTSDPLYIVDGMPVGGIDYLNPNDIQSVEILKDASASAIYGARGANGV
                     VLITTKQGQKGKFSVNYDYSMGYQNAWRKVSLLNAKEYAIIMNESYANDNRPIPFPDV
                     NAVVQAVGNGTDWQDELFYKNAPTYNHQFSVSGGNENSTFLSSLSYTYQDGIVAKGKS
                     NYERYTYRLNSTHKIGKFSMGNNLAYTNKVTRGIDPNAEFGGPLAKAVNMDPITPVKN
                     PDGTWGISNYATQEVVNPVAYLSVLNSNYSENKLVGNVWGELEIIKGLKAKTNFSIDY
                     ANGSSRTFVPVYFLSDIVKATTSEASGTVNKWFTWQSENTLTYNNTIGQHNFAAMVGM
                     TANKYYHEYIGGKKNDLIFNDFEHAWINNGTDEDSYRSWGGADEHALLSYFGRVNYDF
                     DGKYVFEGVLRADGSSNFGSNNRFGYFPAFSAGWVMSKEEFLQGFTPLSFLKLRFGWG
                     RNGNEAIGAFKYTSLIGSGSKYTFGTDEVITLGSNPVGISNPDLKWETSEQTNVGVDA
                     RFLSDKLTFTLDLYNKKTKDLLVVAPIPGYIGNAAPTVNGGSVSNKGIEFELGYKTLI
                     NDLGINANLSGAYNKNEVLSINNSEGKIYGAGLAVGMYNICVMETGYPIAYFWGYKTA
                     GVFQNEQQIQNYKSSDGTVIQPNAKPGDLIWVDKNDDGTIDDKDRTMIGNPYPDFTLG
                     INLGLTYKDFDFNMFWYGAFGQEIFKGTRRYDLPMSNWDASVLERWTGEGTSNSHPRV
                     TINDPNQNYFRVSDFYIEDGSFLRLKNITLGYTLPQKFSQKVKISKLRIYATAVNLLT
                     FTKYSGFDPEIGAKWALDVGIDRNIYPQPRTFMFGVNLSF"
     gene            37151..38734
                     /locus_tag="PK049_00170"
     CDS             37151..38734
                     /locus_tag="PK049_00170"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOK60002.1"
                     /translation="MRKIKNISIILFMALGIVACTKDFLELQPLTDRVEDNFYKTEKD
                     AFEAVVSIYDVLQWQTGGGYHPWDLVSNILSDDAFAGGSGPGDRPGLVRMGKLSNFVT
                     DEEPLGIWKDRFSGIYRANLFLSKVDGVDFKTEGLKTRLTAEARFLRGYFYFELVQFY
                     GNVPLILKPLAPSEYQQSAADPADVYNQIASDLYYAYQNLPATITAAEKGRASKWAAG
                     ALLARVYLFHKGYGKGVLNITTDLKADDKTFTETDIETIIDDIVENSGHGLVANYANL
                     WGVDNENNNESIFEIQHTQKADWGDWGWRNGTEGNWAVVMTGFRGVEDPIYESGWSFQ
                     PASQSLADEFEPGDSRYSVSILDAAAESLVYKPENCYQHTGYAFKKFYPRKADRPATN
                     DALNWPYNRPVIRFADVLLMGAELYLDNNLGKAQDYYSRVRKRAFGDAHVAPTLTNDQ
                     AGLDLIYHERRVEFAGEGLRYWDLLRRGLTYAETKINAAAGASPLDETFSSATKGLLP
                     IPQTEITLSGNKLKQNAGY"
     gene            38751..40511
                     /locus_tag="PK049_00175"
     CDS             38751..40511
                     /locus_tag="PK049_00175"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOK60003.1"
                     /translation="MNKRHIIFSALVLVGLTWFGCEPMELDKPNIGNAPTAEQLDFTI
                     TPGDDAFHFVITNTSTVKGIANWDLGNGIKAVGNSVTAYYPLAKTYNITLTLYASGGS
                     TSKTKEFEQTETDWSYLESPIITALTGGVDAPNGKTWVIDSLSEAHLGVGPADSYSPV
                     WWAAAPLAKSGHYLYDDEFTFKLVGFTYTIDTHGKTHVNHDGNADEDGLSAGYYVSSF
                     WNDAYDIDMNTNDAARGELTWSISEENGKYYIQISSQSGNISYDDGNPRKYEVLEWNE
                     NFFHLRSVGGAARYHKIIPKGYVRPTISYTLNVTATANPNEYTFALEDLTVPSSFTIS
                     NITYDFGDGSSYQTTNGAEVVTHTYMRKGNYNTKVTVTANGQDFVKTSTVAVAANHPT
                     YQEYLLDAMVMYNDFGETTLVPMQVDQSDGGATLEIVTNPNNTLYPNRSAHCALFTKY
                     NSQWANAFTRLPDGYRFDLTKQTVFKVLVYGKAGDQVLLKLENTDRGGNAWQTGTHDL
                     IYTIQNDNKWEIAEFNFAGVGAGWDWTGDIFTGDVTTDSRFNKDFYNVVRIMINPGNN
                     GGTFSVHLDDLAGPHIEGLK"
     gene            40573..42819
                     /locus_tag="PK049_00180"
     CDS             40573..42819
                     /locus_tag="PK049_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005830586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOK60004.1"
                     /translation="MMRLKLIIAVVTLAAWSCSGNKATMDHSGGKLSIDPAIERKVDS
                     VLKLMTIDEKIGQLNQLTGNGETTGPITFATEYQDAIRKGEVGSMLNVNGAAYTYKVQ
                     KIAVEESRLGIPLIFGYDVIHGYKTIFPIPLGEAACWDLDAIEKSARIAAIEAAAAGQ
                     HWTFAPMVDIARDPRWGRVMEGAGEDPFYGSLVAKARVKGFQGSSLADPSTIVACAKH
                     FVAYGAAMGGRDYNTVDISNRTLHEVYLPPFKAAIDAGVGTFMSSFNELNGVPVTGNK
                     EMVKDILKDEWQFNGFIVSDWGSIREMLVHGIAADDYQAADLAMNAGIDMDMEGHIYI
                     HQLKKLLADGKITEKQIDDAVRRILRIKFMLGLFDDPYRYCDTIREKELILNPRHLEI
                     AREVARKSMVLLKNDGVLPLSKKLNTVAVIGPLADNTEDIIGTWSARGEGKDAVSVLT
                     GIRQKLPNTKVIHAKGCDITGDSKAGLTEAIATARKADAVVLVVGEAAMMSGEALSRA
                     FLDLPGVQKDLVLAINELKKPMVVVLMNGRPLTIEWMDNAVPAILEAWFPGTMGGPAV
                     ADVLFGDYNPSGKLPITFPRNVGQIPLFYYHKNTGRPRVETERYTSKYIDSPNTPLYP
                     FGYGLSYTSFDYSNFGVSTNEIGMTDTLKVWVDVTNTGNYDGEEVVQLYIRDMVGSVT
                     RPVKELKGFEKVFIKKGETVKVMFKLTASDLAFYTRTMEFKAEPGDFKVFVGGNSRDL
                     QELSFRLK"
     gene            42935..44755
                     /locus_tag="PK049_00185"
     CDS             42935..44755
                     /locus_tag="PK049_00185"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HOK60005.1"
                     /translation="MSRKFINSIIALLLVVPFTSLAQVKVLVWNDEFNYTGLPATDKW
                     GYDVGGGGWGNNELQYYTNARTENARVENGNLVIEARKEQYEGSEYTSARLVTRYKGD
                     WLYGRVETYAKLPSGTGTWPAIWMLPTNWVYGSWPKSGEIDIMEYVGYDPGVVHGSIH
                     TEAYNHVLGTQKTATISVPDAETAFHLYALEWTPEKIDIYVDNNKYFTFDNEHKDYKT
                     WPFDQVFHLILNIAVGGNWGGAQGVDPNIWPQKMYVDYVRVYQYIDVSALKVSGPEYV
                     SPNQTGVTFSIQNVADATFSWSVPAGATIVSGQGTNSIVVNWGDVQGDVSVEITHPQA
                     GGTYTKTVKTAVIPTGDRFSLLNVKENGLIGWAGLNTSPNTITLSAVDTLLKVKYNIT
                     KPEDFPYFTYTFQNPVDMSNLTVLNVEMMTYNQSKSVVMRADLFDTDGRLTDVSPVFR
                     FYPVEPHGVFHYYSFDFNNNWGSNTPEYGKQVNYHQIAGVRFYVNYGVYGKAASDSLW
                     ISDVVLSNTLLTIFSPSENKISRFDIYPNPASTYIKINSSALKGNVKIQVINIAGVLV
                     KEIIENSDGQINISDLPNGFYSVRIITHTNLLQTGSFIKL"
     gene            complement(44758..47643)
                     /locus_tag="PK049_00190"
     CDS             complement(44758..47643)
                     /locus_tag="PK049_00190"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOK60006.1"
                     /translation="MRKLIFIAFAIFATNAFSQINTAGLPIIKHYPKSVYGAGTQNWS
                     ICQDSRGFLYFANNNGLLRYDGNSWDLFPLPNNQLVRSVYCSGDTIFVGAFEEIGYFV
                     YNRQADMVYHSLISLVPKTDVNFDEAWRIFKIGPKYIFQTFSHILEYSKGEMRVFRAP
                     ELLQFSFVMRGNYYVQGKEGNIYRYQNGSFDRFNDNGIFNGKQIWACFEISGGKTLLA
                     TINHGVWIFDGITFTPWKGQVNDLLKAYQVFSASRVSGGYIAFGTIQNGLIITDDQGN
                     LVKFINKSTGLQNNTILSTFVDNANNLWLGLDNGIDYIELNSPLSFIGEGLGLEGAVY
                     ATKIFDGKIFVGTNQGLYVASWPIGVFVDGTKPFRMVPLTKGQVWSLFESDGKLYCGH
                     NFGTFIIDKGLNVRLISPEEGSWMFLPIRSVPDLMIVGKYKGLHLYSKANGEWRYLKK
                     IAGFNESSRLLVEDDNESIWMAHGYKGIFKLMLNQSKDSIRFSTLYDNTRGFPGKFAI
                     NVEKIGNQVVFLTSQGVYRYNSLTDNMEPYQELNQIIGDATGVRRMVEDEKGNIWILK
                     SNGLLKLVREADGAKRVYQTPVSRFGANLVASFENIYVYNHSNIFIGTENGLVYYNSL
                     YNPELQKNELYCAVKSISSTGKHESVIFNDYAVTGNGGLYIPFNQNSIRVTAAAPVYN
                     AGEITFSFLLKGYDNDWSPWQRDNAKDFSQIPYGEYNLMVRVRDAMGRISEPTVVHIQ
                     VGLPWYFTWYAFILYLVLLVAAFYTLRILVRRKVQRATARVKEQKNLELKVKEEEHKR
                     QVLEAEREIIRLKTENLQVQIEHKNRELAATAMQIAHKNDFLNRLRLRLEVIAKSINP
                     VSQKEVLDLIRNIDNDLKMDKEWQRFEHHFDEVHSGFIKKLKEMYPELTPSELRLCAY
                     LRLNMTTKDIAQILNISVRGVEISRYRLRKKLKIESDTNLVDFMLNL"
     gene            47921..49444
                     /gene="gpmI"
                     /locus_tag="PK049_00195"
     CDS             47921..49444
                     /gene="gpmI"
                     /locus_tag="PK049_00195"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOK60007.1"
                     /translation="MIKSKVLLMILDGWGIGNKDKSDVIYSTPTPNIDRLTSKYPNAQ
                     LLTSGENVGLPDGQMGNSEVGHLNIGAGRIVYQDLVRINKDIKEGKLAQMPVLVNALQ
                     YAKQNNKAVHFIGLVSDGGVHSLDKHLYKLCDITKEYGLSKVYIHALTDGRDTDPQSG
                     LGFVKSLEDHLKSSNGVIASLVGRYYTMDRDKRWERVKEGYDLMVHGKGKPTTDIVRA
                     IEESYAEGVTDEFIKPIVRVDAEGKPVGLIREGDVVICFNFRTDRLREITIVLTQKDM
                     PEFGMQTMPLHYVTMTRYDDTFKNVNVIYEKDNLLNTLGEVVSNQNLKQLRIAETEKY
                     AHVTFFFSGGREQEFPGESRILIPSPKVATYDLQPEMSAPLVKDAIVTELHKKTHDFI
                     CLNFANGDMVGHTGVYTAIQKAVQTVDACVGEVVDAATANGYEVVIIADHGNADYALN
                     PDGSPNTAHSLNPVPIILVSDRFKKVDNGILADVAPTILTMMGLPIPQEMTGTVLCQ"
     gene            49460..50035
                     /locus_tag="PK049_00200"
     CDS             49460..50035
                     /locus_tag="PK049_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HOK60008.1"
                     /translation="MIEIDNKVVSSEILEQRFCCNLPRCLGKCCVHGDSGAPLTPNEA
                     LTLEQILDKVKPYMTPQGIATVQEQGVALTDSDGDLVTPLIDGKECAFTVFENGIATC
                     AIEKAWNNGIVDFRKPISCHLYPIRVKEYSTFTAINYHQWDVCAPARELGEQIDLPVY
                     RFLKDAIIRAYGKEFYGQLEDAAKLLSLRTE"
     gene            50057..51421
                     /locus_tag="PK049_00205"
     CDS             50057..51421
                     /locus_tag="PK049_00205"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HOK60009.1"
                     /translation="MDTIKAYIEENKQRFLDELFGLLRIPSISSISEHKPDMVRGAEY
                     WRETLLKAGADRAEVMPTPGNPVVYGEKIIDPKLPTVLVYAHYDVMPVDPIELWKSQP
                     FEPEIRDGKIYARGADDDKGQGFMHAKAFELMVKTNTLPCNVKFMIEGEEEIGSPNLG
                     KWCQDNKEMLKADVILVSDTSMIGKDVPSITTGLRGLAYVEVEVTGPNRDLHSGLFGG
                     AVANPANILAKMIASLHDENNRVTIPGFYDDVLEVSPEERADMARAPFNLDDYKKALD
                     IDDIHGEAGYTTMERTGIRPTLDVNGIWGGYIGEGAKTVLPSKAYAKISMRLVPNQNH
                     EKIAQLFKKHFESIAPKSVKVKVTPHHGGQGYVAPTNTPAYRAASRAIEDVYGIKPVP
                     FRSGGSIPIISTFEQVLGIKSILLGFGLESDAIHSPNENYPLEQFFKGIEVIPRFYKY
                     FSEK"
     gene            51566..52069
                     /locus_tag="PK049_00210"
     CDS             51566..52069
                     /locus_tag="PK049_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60010.1"
                     /translation="MEKLKMIFALLMGLAVIVSCKQETKKQAATTDEIVQQSIKSWME
                     NRKEEYPNYVPLDFGKLTPRYRFNSRTHSIEIALNTEKSKDNPNQNTLDSLKRLLNEN
                     STDFLGYTITHQFTTKGIDGEVKKDEKLFFIDPQNRVITVLNSDAWDLIMDKELFFRP
                     ESPDSVK"
     gene            complement(52095..52167)
                     /locus_tag="PK049_00215"
     tRNA            complement(52095..52167)
                     /locus_tag="PK049_00215"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(52132..52134),aa:Phe,seq:gaa)
     gene            52256..52957
                     /locus_tag="PK049_00220"
     CDS             52256..52957
                     /locus_tag="PK049_00220"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60011.1"
                     /translation="MKQNIFSILKSVTLLVVITYSNQGALYAQNQDCKVLLPEISAAY
                     TGKCKNGLAHGKGKAWGTDSYEGKFKNGLPHGFGIYTWANGDKYEGNFYNGQMDGKGI
                     FKGKINGKDSVYTGFWQKGVLHHKILPPKYQIINARNVQRYTITKTGSENRILIAFTQ
                     NGTTNNLVTNLQIVCDTGSPFKLGEKSGFENILFPVNCKITYQTPNALRTVWYDVTFE
                     FTINEAGEWAINLFN"
     gene            complement(52976..53605)
                     /gene="pyrE"
                     /locus_tag="PK049_00225"
     CDS             complement(52976..53605)
                     /gene="pyrE"
                     /locus_tag="PK049_00225"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOK60012.1"
                     /translation="MDSKVIANKLLQINAIKLNPANPFTWASGWKSPIYCDNRKTLSF
                     PEVRNAICDGFVKAIKERYPQVEVIAGVATGAIAHGMLVADRLNLPFIYVRSAPKSHG
                     LANQIEGDYKPGQRVVVIEDLISTGMSSLAAVDALRDAHCEVLGLLAIFSYQFDQAVS
                     SFAKAGVEFATLSNYSVLIEAVLEQGSITPTELELLKSWRQNPEEWGRR"
     gene            53604..54224
                     /locus_tag="PK049_00230"
     CDS             53604..54224
                     /locus_tag="PK049_00230"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOK60013.1"
                     /translation="MTLNYTIFFYNRKFIITSDPQEVLIADSTFFCWGDTKPEDISKL
                     IDFFQSHTEVSTLFACTSDPKTTMKHFTNSFNHIEAAGGVVCNDKNEILLIKRLGRWD
                     LPKGKVEPNEPIHDAALREVSEETGVKGITLGKQITTTYHTYTLNGKQMLKSTHWFNM
                     YTPKCDTLKPQTEEGIEQVKWVKRSDIFVYLADSYSSLLSVLKELD"
     gene            complement(54221..55636)
                     /locus_tag="PK049_00235"
     CDS             complement(54221..55636)
                     /locus_tag="PK049_00235"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOK60014.1"
                     /translation="MAKFKFLLLFLLLFIGVECKIGAQEAMIFGNAAEYKGSDIIFYT
                     YSDYITETEYEVGRCKVERDGKFNAKLKVLETSFVFAHLGVYRIYFFAEPGKRYELVL
                     PPREDKTEAQRLNPFFRETDLQVGIKNVDRNDINFLINAFDLRFNQDFDQIVQDAYRG
                     RQHNIDSLITRIEEKFAGTGNQFFDAYREYRYGLLRQITFIKKSTATSKELFLNRPIL
                     YRNPAYMELFNLVYDKYFLFFSRTSQGNAIFDDISRYKSLGRLKRTLATDDVLSNDTL
                     MEMVILKSLHDEFFSDNFSRSALLTILDSIYRTTTIPEHVIVAENIRNRITKLLPGFV
                     PAPFSLNDVNGREVSLDRFKGKYVYLNFCTTTSYTCLKEFVQLEKISKQYKKHLEVVT
                     ISADDNIDDLKSFLENSKYDWTFLHFGNKPEILKDYDIRVYPTYFLIGPDRKMILSPA
                     PSPEEGFERRFFQLLRSKGEI"
     gene            complement(55641..56111)
                     /gene="coaD"
                     /locus_tag="PK049_00240"
     CDS             complement(55641..56111)
                     /gene="coaD"
                     /locus_tag="PK049_00240"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOK60015.1"
                     /translation="MEKVALFPGSFDPITVGHESIVYRSLSLFDKVIVAIGYNANKKA
                     FFSIEKRIAMIQKVFAQEPRVQVISYEGLTVDLCNRLGVRYILRGLRTSADFEFERAI
                     AQVNKLMHPNIETVFMLTAPQHTPVNSTIIRDILLHNGDPSQFIPSAINIKDYL"
     gene            complement(56231..58687)
                     /gene="lon"
                     /locus_tag="PK049_00245"
     CDS             complement(56231..58687)
                     /gene="lon"
                     /locus_tag="PK049_00245"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOK60016.1"
                     /translation="MKKKFGLSILNQQMDDTNDSEVNFIPILAEGEVPEVKKADIPDV
                     LPIMALRNTVLFPGVLMPITVGREKSLNLIASLPPKKKIIGVLSQVDARIEDPSSGDL
                     FKVGTIAQVIKVLELPDGNSSVILQGLSRFRVDQFVSEEPYFMARVLELAELLPNEDD
                     TEFIALTESIRDLTIRIIHLSPNIPKEASFAIKNIDNPKMLVNFISANSDIKTDEKQA
                     LLEEHDLKRRAEQLLRILTRELQMLELKHDIQNKVKHDLDQQQREYFLQQQMKTIQDE
                     LGGNPIEQEINELKERAKAKKWSKEVAEHFDKEVNKLNRMNPMAGEYSVQLNYVQLLL
                     DLPWNEYTNDNFNLDHAKNILDEDHFGLEQVKERILEHLAVLKLKGDLKAPILCLYGP
                     PGVGKTSLGKSVARALGRKYARISLGGMHDEAEIRGHRKTYIGAMPGRIIQSLKKVKS
                     SNPVIILDEIDKVGKDFHGDPASALLEVLDPEQNSTFHDNFVEVEYDLSKVLFITTAN
                     TVATINPALRDRMEMIEVSGYSLEEKVEIAKRHLLPNQLKEHGLNSDALELSDKVLAK
                     VIQEYTRESGVRNLNQKIAKLVRYAARMIASEQKYNPKLTNKDIEKILGVPRYTKELY
                     QGNDIAGVVTGLAWTEVGGEILFVETSLSRGKGNLTLTGNLGEVMKESAILALEYIKS
                     HADYLGIKHEVFEKWNVHIHVPEGAIPKDGPSAGITMVTSLASAFTQRKVRANVAMTG
                     EITLRGKVLPVGGIKEKILAAKRANITDIILSADNRKDVSEIDATYVKGLKFHYVSNI
                     HEVLDLALLNEKVDKPMNID"
     gene            complement(58901..60091)
                     /locus_tag="PK049_00250"
     CDS             complement(58901..60091)
                     /locus_tag="PK049_00250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlmU family protein"
                     /protein_id="HOK60017.1"
                     /translation="MINTIVLFDDQRRDDLLPLTFTRPVAEIRMGILTISQKWSMLTG
                     ASVHYLTQDYLQTKYPNAPKGEPVLLVNGGICPDSNLVDKIRALKPNQAIVKGDTLIA
                     AMLNSTVDTLPAAGLFEKTFEYPDDILEVKYPWDIFRNNGEAIVRDYSLITKGRKSQP
                     ISATNTVLNPDNVFIEEGARVECAILNAETGPIYIGKDAEVMEGSIVRGPFALGEHSA
                     LKMGAKIYGPTTVGPYCKVGGEVNNSVFFAFSNKAHDGFIGNSVIGEWCNLGADTNNS
                     NLKNNYASVKLWSYRKKGFVDTGLQFCGLIMGDHSKCGINTMFNTGTVVGVSANIFGD
                     GFPRNFIPCFSWGGAAGFTTYQPQKAFETARIMMSRRDLPFTDIDKEILLKVFELTRE
                     YRMY"
     gene            complement(60194..60454)
                     /locus_tag="PK049_00255"
     CDS             complement(60194..60454)
                     /locus_tag="PK049_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HOK60018.1"
                     /translation="MKKGIHPENYRLVAFKDISNEHVFITKSAVNTKDTITIDGVEYP
                     LVKVEISNTSHPFYTGKVKLVDTAGRVDKFMNRYKKHMDNKK"
     gene            complement(60566..61321)
                     /locus_tag="PK049_00260"
     CDS             complement(60566..61321)
                     /locus_tag="PK049_00260"
                     /inference="COORDINATES: protein motif:HMM:NF024221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HOK60019.1"
                     /translation="MNLTKSSNPALSDRIFSKAGYASGAEVMTLQGTLNKALLMLALV
                     ILGAAYTWRIFFESVNPASVVPWMFVGGIGGFITSLVVIFAPKTSPYTAPVYALLEGI
                     FLGGISAYFASSYGAGIIMQAIGLTLAVFFLMLLAYRSGTIRATGKFKVMVLAATGAI
                     ALTYFISFVLSFFGISLGFIHGSGTFGIIFSLIVVGVAALNIIIDLDFIDRASASGAP
                     KFMEWYGAFGLMVTLIWLYIEILKLLAKLNRRN"
     gene            complement(61386..62045)
                     /locus_tag="PK049_00265"
     CDS             complement(61386..62045)
                     /locus_tag="PK049_00265"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOK60020.1"
                     /translation="MATLEKVLEIKSQIIHRASGEVAENMRMAGIIYKVNYGVPIPEL
                     KELAKPYKGDHDLALELYAEDIRECKILASLIADPERLTGEQIDEWAAEFTNPEIVEQ
                     VCGNLFWKSEFALSRSIEWCLSNDELLRKAGLLIIARKASDTAIKESLLEPYIGIIEN
                     MAEEMTDLTQSAVQFALREIAKRSPELAARVSETASRMTESENELAAWVGNELLFELT
                     E"
     gene            62752..62928
                     /locus_tag="PK049_00270"
     CDS             62752..62928
                     /locus_tag="PK049_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60021.1"
                     /translation="MGTPRVELPRDTIRHACGVTHVVSLTKPIPTFHPRPIAGSGFYA
                     AAYAIPCWLRNLID"
     gene            63288..63464
                     /locus_tag="PK049_00275"
     CDS             63288..63464
                     /locus_tag="PK049_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60022.1"
                     /translation="MGTPRVELPRDTIRHACGVTHVVSLTKPIPTFHPRPIAGSGFYA
                     AAYAIPCWLRNLID"
     gene            complement(63677..64927)
                     /locus_tag="PK049_00280"
     CDS             complement(63677..64927)
                     /locus_tag="PK049_00280"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOK60023.1"
                     /translation="MRFVAMLTIGIVMARLGFGKTAIGNYEAVLFISGLLTTFWVTGL
                     IQALLPLYTRSNDISKDDRLFNAFMVIFGLTVLACLFATVFTATITGFAAINHSLFPL
                     MLLYMLLSTPSTMAEYIYFLHGNYKAMLSYGVITYGIQIILVVLPVLFGLDVAFAVLG
                     LIVVSAVRFIWLISLLVRNSKFRVNVDFLKDYLSDGIPLSLKFLISSSGLYIDQLIIG
                     YYYDSSTFAVYRFGAREIPLATILAASLSNSMLADFADRQRLGNTLLNLKLESSMLMH
                     ILFPTSIAAILLSRWLFPLVFGAEFQESANVFMIYCLIVLSRVIFPQTVTQGFRKNVA
                     VLSISIAEMIMNIILSLLLVKYFGINGVAAATVIVYAVEKVLLMAYNSYQLKISLVSY
                     VPVKIFLAYSLLTLSVFAFSWFFL"
     gene            65100..65175
                     /locus_tag="PK049_00285"
     tRNA            65100..65175
                     /locus_tag="PK049_00285"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:65135..65137,aa:His,seq:gtg)
     gene            65408..66937
                     /gene="amrB"
                     /locus_tag="PK049_00290"
     CDS             65408..66937
                     /gene="amrB"
                     /locus_tag="PK049_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013065694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOK60024.1"
                     /translation="MYNLLNRSKKLCAFTLTVLIVTETSCNGQNMNNPNDFDRQPAVA
                     GQFYPGTKAQLEKEVKSYFDSAQKVLDAQPLALIVPHAGYVFSGQVAASGYRQIDRDK
                     KFKHIFLIGSSHTMFFTGAAVYTKGAFITPLGRVPIDPLANELVQNHKVFTDDVKPHV
                     KEHSLEVQLPFLQYWLKNDFSIIPIIIGGESQATVTLLAEALEPYLNPDNLFIISSDF
                     SHYPSYEDARHTDDDMAHAIVSNSPVEFMKAKIRNENAGIDDLATAMCGWTSGLTLLK
                     ITEGKPGITYKTIMYRNSGDSPYGGKDRVVGYWAIAAIQQQAKTVDFNLTDNDKRELL
                     KLARKTITEYLSGKTPTPPDESKLSSTLKTNAGAFVTLHNHGKLRGCIGNFSTTTPLY
                     RVVMAMAISAATEDYRFESVTADELNDIDIEISVLTPMKRISDIKEIELGKHGIYIKK
                     GGRSGTFLPQVAKETRWSLEEFLGHCARDKAGIGWDGWRDAEIFTYEAIVFDEKELGL
                     K"
     gene            66948..69365
                     /locus_tag="PK049_00295"
     CDS             66948..69365
                     /locus_tag="PK049_00295"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60025.1"
                     /translation="MSSPYLLIPISLAITIYYLLGSLMVQAGIISFSTHRKFWNFALL
                     FVFLGCAILGILLIININYKLGWSFVKVMLQWHVQLGIAMSVVAFIHTLWHRGYFSKY
                     LLFRGRQTKTTTVNTKQQLVKSAFALGLLSVSLQVLLIRQFTKVFQGNEFLITWIIGI
                     WMLLSAAGAIAGSRTANGINPQKLTRMALGFMFLSAIIFIFLSGEIRQTFFPSGVLIP
                     PYHVIVMIVVMMTPTAFPSGMVYALLTKQIKSEYPSIYAYEALGSLAGGLLLSLVIIW
                     IFNTYLAAIAVGFLASLIIAYPLKNIARLAIPMSLLTIGISVKTLNFDIKAESMLFPG
                     QKVLSVTDSPYGSITVTGSEDQFNFFENGTMLFGTQNTIYCEEVVHYTISQRYMPSKV
                     LLVSGGYAGLIDELKKYKQVKKVDYVEPNPHLLRMSGNYCKVDFPDNTKIINSDIRGF
                     LRKSSEKYDIAIITTPEPTSLEQNRFFTIEFLELLKKHLTADGVLSVSLSGIGNYPSA
                     PRQKAYTSIAATFKSVFRNVEVISGERDYLLASDSTIRIDMAQLLSEQNIGEANLYVR
                     PDYINDEHIAQRNHFFHEQTQSTLKINTDNHPWPVLQNTLGYLSMFGDKIWLLMVVGA
                     VLLLIPFFVTNYHLRAMYAVGFAGSAIQTLYLLTLQIAAGLLYGTLGAMIALFMGGLA
                     LGSMAYGKVISIKPKHIKISLIGSFIILFALFITMMHVEIWLLIAIVSAGILTASFSV
                     GYLYISITENSNSNPNQPAKTYAADLMGSAAGIVIVTLLLIPSIGFLSSIAVLLVFIG
                     IYLFLKV"
     gene            69378..70502
                     /gene="amrS"
                     /locus_tag="PK049_00300"
     CDS             69378..70502
                     /gene="amrS"
                     /locus_tag="PK049_00300"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOK60026.1"
                     /translation="MEKISRREFLKRSLLATGGFMALRYLSLSAFGLDDKPWKWSREA
                     MFYTPTPRGMRCNLCPNECNIRPGETSQCRNRVNYNGKLYTIAYGNPCAVHIDPIEKK
                     PLYHYYPGSKSFSIATAGCNFGCLNCQNWEISQSSPKDTRNAELFPEQVVEQAKRNGC
                     KSIAYTYSEPITFYEYMYETAGIAQKKGIGNVLVSNGYINEEPLRELAKRIDAANIDL
                     KSFSNEIYLKLNAGTLEPILNTLRILLEMNVWVEITNLVIPTWTDDYAMIRKMCQWLA
                     SNGFSEVPLHFSRFFPLYKLTQLPQTPKSTLIKAREIAQSCGLKYVYIGNTALPGSGD
                     TICPSCGKVVVERKGYNVALVNINNGKCGWCGAKINGRWS"
     gene            complement(70767..71006)
                     /locus_tag="PK049_00305"
     CDS             complement(70767..71006)
                     /locus_tag="PK049_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60027.1"
                     /translation="MRVTKRTTLEKKSFSVKKFKQGDSLLIIYPVGCSKLSIIYKPYP
                     TKEEFNKCNDYGYYVNGLLYSKQEYEKLFGNNGTK"
     gene            complement(71843..72163)
                     /locus_tag="PK049_00310"
     CDS             complement(71843..72163)
                     /locus_tag="PK049_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60028.1"
                     /translation="MTREEFEKKFKIIQIFVAKAGFTLEAISRRYYGTLDEKKNPDGT
                     ITYYAKVHVKDKDNDSIIYSMSNDRTELQYKMDSLVVLNVYKDLHKKDGVHFIRYGMK
                     YYLN"
     gene            complement(72256..72342)
                     /locus_tag="PK049_00315"
     tRNA            complement(72256..72342)
                     /locus_tag="PK049_00315"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(72306..72308),aa:Ser,seq:gct)
     gene            72804..74024
                     /locus_tag="PK049_00320"
     CDS             72804..74024
                     /locus_tag="PK049_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008641672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOK60029.1"
                     /translation="MEFELYTLQNGIRLAHKRTDSPVAYCCIMVNTGTRDEFDHEHGL
                     AHFIEHVIFKGTKKRKAFHIMSRMEDVGGDLNAYTSKEETVIHATFLKQDFARAVELL
                     ADIMFHSTFPEKELKREKEVIVDEINSYHDSPSELIFDDFEELIFPNHPFGRNILGTK
                     KQLGKFTRKHIVDFMGRTYNTDEMVFCSIGNIPFNRIVKLAEKYFGGIHPNTRNYKRL
                     PITEYLPQRKTVKKSTYQSHCIVGTTAYDLHNPNRIAMHLVNNILGGPGMNSRLNLAL
                     RERHGLAYNVESSYTPYSDTGIFTIYFGTDKADTDKAIDLILQELDGLRNKRMGILQL
                     SKAKKQLIGQLAIGAESGENLMISTAKSILVYERPDNIQNVFKRIEEITSLEVMEIAN
                     EILSPNRLSMLIYK"
     gene            74027..74668
                     /locus_tag="PK049_00325"
     CDS             74027..74668
                     /locus_tag="PK049_00325"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HOK60030.1"
                     /translation="MYTTNHEIDKYLESHTTPVDEVLHELTRHTYLTTYNPRMISGPV
                     QGKFIEMICQMVRPNRILEIGTFTGYSTICMAKALPAGKYIDTIEVNDELQETIEHYT
                     AKAGVSSKVNLIIGNALTVIPGLPHTYDLIYIDGEKKEYPQYLNLCTEKLNAGGFLIA
                     DNVLWNGKVADPTCTDEQTKAIRQFNKMVQENTTLENVLLPIRDGMMIVRKKF"
     gene            74734..75624
                     /locus_tag="PK049_00330"
     CDS             74734..75624
                     /locus_tag="PK049_00330"
                     /inference="COORDINATES: protein motif:HMM:NF014646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOK60031.1"
                     /translation="MAQYTIRQMELLSGIRASTIRMWEKRYNIFSPQRTDTNIRRYDD
                     NDLRFIINLSLLLKRGFRISKLASMSLEELSDLASKFVSDHRMGNINLEPMITATIQY
                     NETELLKHISEWSEKHGIEYTFENLVLPYLQRLGDLWQTGAITTTHEHFASNVIRNLL
                     SHLYRTCKQPEIGKSTPIIFFLPEGEFHEIGLLYFAYIAKNAGYKTIYLGQSTPIDDV
                     IRCSKDLGCKMVFTSLSSFVSADMDEAFEPMKKALPDVEIIATGHAFTEKSGPSYLTY
                     VSSAQDLMKIIARLDPTIIK"
     gene            75873..76370
                     /locus_tag="PK049_00335"
     CDS             75873..76370
                     /locus_tag="PK049_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOK60032.1"
                     /translation="MSQQEFNQALIELEPNLERFAYSLTANREDARDLLQETYLKALT
                     YKDKFEDNTNIKAWTFTIMKNTFINNYRKSVKQSTTFDGSTNQFLINSKPDSFGPDAM
                     YSHSEISKKINELDDEFRIPFQMHTSGYKYKEIAEKLNLKIGTVKSRIFFSRQKLMNV
                     LKDFE"
     gene            76513..77343
                     /locus_tag="PK049_00340"
     CDS             76513..77343
                     /locus_tag="PK049_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60033.1"
                     /translation="MQLKLIVITFLLFPLTLASQPVIDVYFSSLAPESKGPSFSKVEE
                     VHELLIPKSGTPSYFKIVKQYNSYGKIVSEKRYNKAGGIMGESQWEYNAKGELTKKVN
                     RQFMNFKGWINEQILLTYNDTSGVLEEISLFFENQLKQRAQVIPDKDLKITEVLVFNE
                     KNVLTGKERIVYLPQNNTIRVLSYKSNEQFIGATTYPLDPKLPEPPSAIKREFNSHGD
                     IVLEALPNSIMEQGYFYEYTYDSYGNWTEKLTYQCRVTKNNKVRDKKLEYKITRKISY
                     "
     gene            77725..78075
                     /locus_tag="PK049_00345"
     CDS             77725..78075
                     /locus_tag="PK049_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOK60034.1"
                     /translation="MKSNYTEEQVKLARYAKALSHPVRVQILQILSQQSCCYSGDIVN
                     DLPIARSTLSQHLTELREAGLIQGEFNPPKIKYCIRPEAWNEAREIFDRFFNSNIWEA
                     VNTNSCSTNAGKSK"
     gene            78104..78346
                     /locus_tag="PK049_00350"
     CDS             78104..78346
                     /locus_tag="PK049_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOK60035.1"
                     /translation="MEIKVLGTGCPKCKALEQATQNAINELGVQATITKVEDIVDIMN
                     YGVMRTPALVINEKVVLSGRVPDAKELKEIITKSQQ"
     gene            78349..78780
                     /locus_tag="PK049_00355"
     CDS             78349..78780
                     /locus_tag="PK049_00355"
                     /inference="COORDINATES: protein motif:HMM:NF040494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrophenyl compound nitroreductase subunit ArsF
                     family protein"
                     /protein_id="HOK60036.1"
                     /translation="MKALKYFLLAVASLTIVSCMGNSGEKNSIASTDGSGNPVTVYYF
                     HGEHRCPTCIAIENETKATFEINLKKEAEEGKVELQVINADEGRNKQICEKYGVYGSS
                     LLLVKGDKVINLTNMAFGTARRNPDKFREELSNEVKKLIKG"
     gene            78783..79484
                     /locus_tag="PK049_00360"
     CDS             78783..79484
                     /locus_tag="PK049_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005655271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic aminobenezylarsenical efflux permease
                     ArsG family transporter"
                     /protein_id="HOK60037.1"
                     /translation="MDFILDFIYSSNIPILTAFLLGLATAISPCPLATNITAIAYISK
                     DIENQHRVFYSGLVYTLGRAITYTAIGLLFFFGASQFKIAGFLNKYGERIIGPALIII
                     GLFMLDIIKIKLGGAGKLTENFGEKAKIGNFLSVLIMGMAFALAFCPYSGVLYFGMLI
                     PITISNAKGLYLPVIYALGTGLPVIIFAYFIAFTVHGIGNLYNKVKTFELWFRRVVAV
                     VFIGVGIYYLSIFLL"
     gene            79506..80849
                     /locus_tag="PK049_00365"
     CDS             79506..80849
                     /locus_tag="PK049_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOK60038.1"
                     /translation="MENKKELQVLFWIIFFFAFAFFMPIDNATFRTAIDATLDLTRWY
                     AREHVVLCLLPAFFIAGVISVFVSQGSVLRYFGANAKKWLAYTVAAVSGTILAVCSCT
                     ILPLFSSIHKRGAGLGPAIAFLYSGPAINILAIILTARILGIEMGIARTVGAIAFSVI
                     IGIAMSLIYRKEEQAKREGQMNIVLPPEKRPMWQTSFHFFTLVLILVFANWGKPTADD
                     TSSAWYYLYTYKWYITGFFGLLLAYSLIYILKIKWQWVAGAIGATLLSVILANMYIAD
                     PKVLPLVPMLVGIIALAFITLKDKNDDENKEWTIASWGFAKQIMPLLAVGVVTAGFLL
                     GSTHDGQTIAGVIPNEWIAYLVGGNSIFANFFASIVGAFMYFATLTEVPILQGLLASG
                     MGKGPALALLLAGPSLSLPNMLVIRGVLGTRKAVAYVSLVVIMATISGLIYGYFF"
     gene            81043..81513
                     /locus_tag="PK049_00370"
     CDS             81043..81513
                     /locus_tag="PK049_00370"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOK60039.1"
                     /translation="MIGLTIVVSVLLVVSLLFDTQKTFKGVTRGVKMFLKLLPTLVTM
                     LALVSIMFFIIPNDTLVSLIGKDSGIKGYLIAAVIGSVSLIPGFIAFPLCKILIVNGV
                     EYSTVAIFITTLTMVGILTLPVEAKFFGWKVSILRNVLSFVAAIAIGITMIFLL"
     gene            81510..82025
                     /locus_tag="PK049_00375"
     CDS             81510..82025
                     /locus_tag="PK049_00375"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOK60040.1"
                     /translation="MKNIIKYFLPILSLLFIGFSYLYDNRAGEQIALNFWMFFKEMIL
                     FLPLMFILIGLFDVWVPREKIEKHIGEDSGWKGTLLVILLASLQAGPLYGAFPFAYLL
                     WIKGCNIRNIFIYLGAYSTIKIPMITFEIGFLGLKFSLLRTLITLPIFILIGFAMEKY
                     LKGKKFEVKQP"
     gene            82040..83113
                     /locus_tag="PK049_00380"
     CDS             82040..83113
                     /locus_tag="PK049_00380"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOK60041.1"
                     /translation="MKPALLAALVFFSKCIVTAQTSDSTFKPNGKPIIQVFGNFDYNA
                     TNNAKKTYGFWFGRAHFGYEYQFSKQISGKIIIDAGRPTTVGQIEVRDTSGNSLDVTD
                     KSKEGSYYTMTLKFASIEWKPNSFVRIQAGGVLQNHYITQEKFWGYRYVAPTFQDKYY
                     GIPSSDLGIIGYFTLNEMLGFDIAITNGEGFRFDQDEYGDVKIAGGIDFNPIKGLQTR
                     FYTDYTKSDNPLKPSEQQLFSIFAGYKFDDKFRIGAEYNYRKNHLNINKHNLYGLSVY
                     GGVSVAKNVELFGRFDQLQANTISGDAQSWYYSGIGNAYIAGIHYNPVKGVNISLNYQ
                     GWKPLDSSVDFQNHIVLSFEYKL"
     gene            83126..83500
                     /locus_tag="PK049_00385"
     CDS             83126..83500
                     /locus_tag="PK049_00385"
                     /inference="COORDINATES: protein motif:HMM:NF020434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HOK60042.1"
                     /translation="MEDNKKSGCLCGVDEYMVLACSGACDLGQITDLVARKLRDNGVR
                     KMNCLAVVGAGIEQSIEAFKSRNILMLDGCPTDCGKRILDRHGFTDYKYFRVTDLGYK
                     KGQTPVTEEVVNKVYEKVEVIY"
     gene            83657..85051
                     /locus_tag="PK049_00390"
     CDS             83657..85051
                     /locus_tag="PK049_00390"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HOK60043.1"
                     /translation="MLRPYFKNKVRIIFSLTCLACQLVLPTFSNANTGGDSTLLEFNI
                     TLRERFEVWDGMNAKNYGDPNGVGSLNDKILFQRIVAGFTFKPTSKIDFAAHIQDSRA
                     FGWSLRDSEYPELFRVKQKNTQEPYYTMNPNEEFFEIYDLYFSVKEIFKGVSLKIGRQ
                     KISFGDKRIFGPGEWGNTGRWTWDAVRLSYRNKNNSLDVFGGGTKTHDPEKVAIPFTE
                     TEHWGGGAYAHIQFGKLAVVEPFYAIKTQGSADYIRKQSFSKHWIGTRVFSDEVNRFK
                     YDFTLTQEFGHENGKTINAFGIIGLLGYQFHGLPAKPTLCLRETYASGGNNDDTRVRT
                     FDPAFGAKDSYYGRMNITSWSNLDDREVVLYLTPSKKFKIEMNYHFFYLPSPDNKTLL
                     GTMKLQAGKHHLGNEFNFFASYNAGKQFQFVGVFGYFWAGDLQPINGTTAQNSNWFAI
                     QAFIKLDKEIHAKH"
     gene            85106..85654
                     /locus_tag="PK049_00395"
     CDS             85106..85654
                     /locus_tag="PK049_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HOK60044.1"
                     /translation="MKIKLTQNGEWHWLTGGIVVSLLSIATYVLFEKLSYKDYPFGIT
                     AGVAYIAASFKYVFVSLADNPVIQKNWTSPAAFVEFIMLFGIILGAYIGAKLSKNYSP
                     EVIPAVWEKYHGTNVWKRFGVVLLGGFCLGLGTMLAVGCTTGNILQGWAHLSLGSMVA
                     GACFFIAGIIVAKLLYPKAGGR"
     gene            85657..86193
                     /locus_tag="PK049_00400"
     CDS             85657..86193
                     /locus_tag="PK049_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HOK60045.1"
                     /translation="MLLQILIGLAIGLIFGYALQRTGVTKYPRVTGMLLLKDFKILKF
                     MLTAVTFSMIGFYMLGNLGVIELHPKALDWGKVVGGLIFGTGMGLLGYCPGTLFARIG
                     EAKKDAWVALLGMLLGILFFAVNVKFFKSNLMTKNINGDISAVLGINQWIIVPIAALV
                     FVAIIYLANKVDDFKKNF"
     gene            86361..86750
                     /locus_tag="PK049_00405"
     CDS             86361..86750
                     /locus_tag="PK049_00405"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOK60046.1"
                     /translation="MKRLVIYLTITAFFIAALSSNTFAQTKSQTSKTERKYKVTFIEL
                     GSVRCIPCQQMQPILKSVEEKYGKQVKVIFYDVWTDDGKQYAKKYNINLIPTQIFLDE
                     NGKEYFRHEGFFPENELIEVLKKKGVK"
     gene            86843..87430
                     /locus_tag="PK049_00410"
     CDS             86843..87430
                     /locus_tag="PK049_00410"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOK60047.1"
                     /translation="MRQPTEVRQEQIKQAVLGIIYTDGLKNLSTRMLAQRIGMSEGAI
                     FRHFPTKQSIIHSIITDVQRDFIEHLRKIATSNELPQKRLEHLLCFTVKYLTDNKGIT
                     LLLFSEASHNNDEELKQALMQIFNTQKKMVSKVVLDGIAEGIWDENIPVENVAMLYMG
                     IPVSLNVELILNKGVLDTSNFCSRMMFLINKMLTR"
     gene            87478..88230
                     /locus_tag="PK049_00415"
     CDS             87478..88230
                     /locus_tag="PK049_00415"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOK60048.1"
                     /translation="MLFIIVFIVISIASFIFSMLGLGGGMVYVPVLNWAGFDMVTVAI
                     PLGLLLNGLNTALVLIPFARKKMVDWKGGAVMAATALVASPLGAMTSEHVPVQLLKFL
                     FAVMVVAAALRTLWASKQEEPETMMSLKKRSIIGFFVGGFAGFIGGMLGLGGGFIIAP
                     ILMMMGYKTKEAAATTAFVVTFSSFSGYLGHVSQGHINWPLTIVVVLAVIIGSQLGGL
                     YMTQKAKSKQVKVVYAVVLLLIATKMVYGILS"
     gene            88242..89132
                     /locus_tag="PK049_00420"
     CDS             88242..89132
                     /locus_tag="PK049_00420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOK60049.1"
                     /translation="MSHDHNHHKVSGKNLGITILLNLFITVAEFLGGIFSGSISLLSD
                     AAHNFSDVLSLIISYTANRLSGQKATLTRTYGLKRSEILAALINSVTLLGLAIAILLE
                     SVKRLYKPIPIDSGWVIWLSLLSILVNGLSVLFIKADSRNSINIRSAYLHLFSDMLTS
                     VAVLIGGLAMKYLQWYRVDALFSILIAIYLLFTSWKIIKASLRIFMQFSPENININEI
                     TKQVESLEGVKNLHHVHIWQIDEHEIMFEAHLDVTNNITISDFEKILEQVKILLAKQG
                     INHVTIQPEYSVTDNKQLIH"
     gene            89151..89756
                     /locus_tag="PK049_00425"
     CDS             89151..89756
                     /locus_tag="PK049_00425"
                     /inference="COORDINATES: protein motif:HMM:NF014693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HOK60050.1"
                     /translation="MINPKDWLEFGQKFHGHKCPAMPMGLRVGAAAMNALGVERAKDG
                     QLIALVDLGDDHCATCYADGLQVILGATFGKGNIKKTHKGKWAVTVIDKATGRAVRVT
                     PKAEAMLANKQTSFFKDYREKGIPASKVPDLVVDPLVNNVMNAPDEKLINISEVFQYD
                     LKEHPHSFNSFVCEECGEMTVMEYGRIKGDKKVCIDCANKY"
     gene            complement(89817..90617)
                     /gene="zupT"
                     /locus_tag="PK049_00430"
     CDS             complement(89817..90617)
                     /gene="zupT"
                     /locus_tag="PK049_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HOK60051.1"
                     /translation="MSNILIAFGLTLFAGLSTGIGSAIAFFSKHTNTKFLSLALGFSA
                     GVMIYVSFIEIFSKARESLVDAIGLHLGSWITVASFFGGMAVIALIDKFIPEQENPHE
                     VMLVEDAESGRKRKGKLMRMGLFTALAIAIHNFPEGLATFTAALSNPNLGIAIAVAIA
                     IHNIPEGIAVSVPIFYATGDKRKAFRLSFLSGLAEPIGAIVGYLLLMPFINGVTFGIL
                     FAAVAGIMVFISVDELLPAAREYGEHHLSIYGLIGGMIVMSISLLMFI"
     gene            complement(90696..93929)
                     /gene="carB"
                     /locus_tag="PK049_00435"
     CDS             complement(90696..93929)
                     /gene="carB"
                     /locus_tag="PK049_00435"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HOK60052.1"
                     /translation="MPLVRKYSKVLVLGSGALKIGEAGEFDYSGSQALKALKEEGIAT
                     VLVNPNIATVQTSQDVADTIYFEPVKPETVRQIIEKEQPDGIFLSFGGQTALNCGVEL
                     YQNGTLREFNVEVLGTPVSTIINTEDRELFVKELNQIGVSTPRSIAVNTVAEAVDAAH
                     QIGYPVIVRAAFTLGGQGSGFCSNPQELKALATNALTYAPQILVEESLKGWKEVEYEV
                     VRDRFDNCITVCNMENFDPLGIHTGESIVVAPSQTLSNSEYHKLRALSIKIVRHLGIV
                     GECNVQFALDPSSENYRVIEVNARLSRSSALASKATGYPLAFVAAKLGLGYGLFELKN
                     SVTHKTTAFFEPALDYVVVKIPRWDLAKFKGASRQIGSAMKSVGEVMAIGRSFEEAIQ
                     KGLRMIGQGMHGLVANQHFNPSDLKLMLENPTDQRIFAIAQAFEQGWTAEDINAITRI
                     DSWFLDKLQGIVELSRHLAGYSAIEQVPSELVVQAKRNGFSDFQLARIIYNPSNNKIE
                     QYQQQVRNYRKSCGIVPCVKQIDTLAAEFPSETAYLYMTYHGNEDDTSVKPKPKVLVL
                     GSGAYRIGSSVEFDWCTVSAVKELRRLGFEAIVVNYNPETVSTDYDVCDKLYFDELSF
                     ERVMDIVEVERPLGVIVSVGGQIPNNLAGRLSKAGVMVLGTSPVSIDMAEDRQKFSAL
                     LDKLGIDQPEWQELTTMEQVQEFVNRVGYPVLIRPSYVLSGSAMNVVSNADDLNEYLT
                     VAAEVSKAHPVVISEFIEGAKEIEYDAVANNGRIFVDAISEHIEFAGVHSGDATLVFP
                     PQRLYLETIRRIRRVAAALAAELKITGPFNIQFLAKNNQIKVIECNLRASRSFPFVSK
                     VMNFDMIAVAVDFMMGVAPKTNPPSMLDIAHVGVKASQFSFGRITFADPVLGVEMAST
                     GEVGCIGQSVNVALLKAMLSVGHRIPQGSILVSSGPLRSKLSLIDPIARLARRGFVIY
                     ATRGTASFLNDHGIPAIAVSWPDEPENAPNSLNLIQNRLVDLVINIPKNLSRSELDND
                     YTIRRSAVDYNIPLITNARLAAAFIEAFLNTDNIQMEVKSYNELVGS"
     gene            complement(93941..95014)
                     /gene="carA"
                     /locus_tag="PK049_00440"
     CDS             complement(93941..95014)
                     /gene="carA"
                     /locus_tag="PK049_00440"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HOK60053.1"
                     /translation="MNQQQKAKLVLEDGSVYTGYSFGYAGNTSGEVVFNTAMNGYPES
                     LTDPSYFGQILVATFPLVGNYGVPEPNPLGGIEQNWESGRIHIKGLVVCEYSQNYSHW
                     NANKSLGQWLNENKIPAISGIDTRKLTKQLREKGCMLGKIIVDGDTDWYDPNADNPVA
                     HVSVAKPITYGNGKKTIVLVDCGVKNNILRCLLRRDVQVIRVPYNYNFLSQQFDGVLI
                     SNGPGDPKVNIETIENIRRLIEMDKPVMGICLGNQLLALASGANTYKLPYGHRGHNQP
                     VRLKGTDRCFVTSQNHGFAIEPSTLSDEWEVMFENLNDGTCEGIRHKAKPFFAVQFHP
                     EASGGPNDTEFLFDEFINLIDNK"
     gene            95330..96517
                     /locus_tag="PK049_00445"
     CDS             95330..96517
                     /locus_tag="PK049_00445"
                     /inference="COORDINATES: protein motif:HMM:NF024865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOK60054.1"
                     /translation="MENEVKQAYLKYFEDYVQRKLEDTLNAFSPDFNMIGTGVDEFSV
                     TYQHSTELFRREFAQAPEPFTYQFKTLKVCPITADSAYVMGLVDMKFLLPTGEFEYTN
                     NRTTAILRKEKGEWKIIHGHWSQPTEMQQEGESVPLSAVLKQNRELKNQLIIKQQELE
                     KQNQQLKEMNQSLSKLFSIVSHDLKSPFNAFLGITDLMLMNFEDDFENKEYFQLRLQL
                     LNDLAHNLYDLTENLLNWAKLNSNAIEPKYREIPIDSLIQKQVNVLRPIWQKKRITLT
                     EEVPSELMVNTDPDMLAVIIRNLVSNAIKFTYPEGEIKIQVSNEPTILSIKIVDNGIG
                     MTAEQLKNLFKNYQSSRGTDNEKGTGIGLFTCKELTNKLKGNLKFISEPNKGTTVIVE
                     LPK"
     gene            96530..97165
                     /locus_tag="PK049_00450"
     CDS             96530..97165
                     /locus_tag="PK049_00450"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOK60055.1"
                     /translation="MAPIPKIVRWIELAALFVGLPVAFYFNNLKLPKSIPLLVVFLIA
                     LFYLLTTKSFSKKSFGFNGYRSWRTLSLRILISLLAISLLSFIFLPSEQLFYLPLHRT
                     ELWLLIMFFYPIWSAFTQEVIYRGFFFHRYTPLFKNDKIVITLNGILFGLLHIIFRNW
                     IAVAGATIIGFVWAYSYFKHRSILVVSLEHAIVGNFLFTIGLGYFFYVPDF"
     gene            97477..97947
                     /gene="bcp"
                     /locus_tag="PK049_00455"
     CDS             97477..97947
                     /gene="bcp"
                     /locus_tag="PK049_00455"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HOK60056.1"
                     /translation="MATTILKPGDLAPEFIGKDQDGKTIKLSDFKGKKLILYFYPKDN
                     TSGCTAEACSLRDGYDDLQSLGMEVVGVSPDSEKSHQNFIHKYNLPFRLIADVDHAIA
                     EKYRAWGEKKMYGKAYFGILRTTYVINENGTITNVFNKVDTKEHVKQILSEINK"
     gene            97948..98970
                     /gene="recA"
                     /locus_tag="PK049_00460"
     CDS             97948..98970
                     /gene="recA"
                     /locus_tag="PK049_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOK60057.1"
                     /translation="MEATENKERKEINKEKLKALQSTIDKIEKDFGKGSIMRLGDKAV
                     SEVPVISSGSIALDVALGVGGYPRGRVVEIYGPESSGKTTLAIHAIAEAQKQGGLAAI
                     IDAEHAFDRTYAEKLGVDVENLLFAQPDNGEQALEIADNLIRSGSIDIIVIDSVAALT
                     PKAEIEGDMGDSKMGLQARLMSQALRKLTATISKTNTCCIFINQLRDKIGVMFGNPET
                     TTGGNALKFYASVRVDIRKLNQLKDGEESTGNRVRVKIVKNKVAPPFRKAEFDIIFGE
                     GISKTGEIIDLGVELNIIKKSGSWFSYGETKLGQGRDAVRQLLLDNPELSNEIETKIR
                     ETLKAQ"
     gene            99186..101834
                     /locus_tag="PK049_00465"
     CDS             99186..101834
                     /locus_tag="PK049_00465"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOK60058.1"
                     /translation="MKKIVTISALLLIILSSCDTILNRHLIKGSDKRKEVHAQFEERV
                     KLIAAYDSSIYKIINSELTTKEREGFEFLYAYMPLSDLAMHSPEYVLKNVQLALKARK
                     EFKWARKVPADIFLHFVLPYRVNNEYTDNAREIFFEELKERLLGLNTYQAALEVNYWC
                     HEKVTYQSTDERTCGPLTSVRSAIGRCGEESTFAVAAYRSVGIPARQVYTPRWAHTDD
                     NHAWVEVWVDGKWHFLGACEPEPELDRGWFAGPAKRAMMTRTFVFGKYNGPEEQLKQT
                     NYYTEINLMPNYAPTRKLTVTVTDSTGNPVEGAKVEYQLYNYAEFYPIATLQTNANGL
                     CTLTTGIGDLMVWAQKDNTYAFSKADRNQNDIYLTLKKEIPFTNLSLTLTPPDEQTIP
                     PYDESTADEHNRRVKQGNEIRSNFISTFIDSASAANLAMEKGISIDETWKWLSKSRGN
                     WNEIYSYIKNLDTKDISVGVALLSTLREKDLRDITTDVLFDHLSRIDSFPALLESRMV
                     KEFDSYILSPRIGREFITPWRSFLQDAFTFEEIGFFRNNPQNIAQWIKEFVTLDTESN
                     YYRVPLSPESVYRIRRADEYSMAIFFVAACRSFGIAARLEPASKKPQYFMKGKWIDVN
                     IADVKETQATTPTGMLIINLDKNSAVEQPKYYTHFTIARFEKGKYSTLDYEESPLFNK
                     FPAALELPEGYYRIVTGNRHKSGAVNCNVYYVRVESGKKIQFTLTIPSTQTEKMVLGT
                     ISTDITIPFIQNPQTIEQITKDKPAIVVIIDPLAEPTKHLLRDISSLCSEFSGIGNSI
                     ALILASGKSAKTDLTSIFDGKPYPNTITLGNDINGQFEAKVGQAVGKQLEYPVVLVVN
                     NNGEIVYHSSGYSINLGEQLLSALKQ"
     gene            101838..102584
                     /locus_tag="PK049_00470"
     CDS             101838..102584
                     /locus_tag="PK049_00470"
                     /inference="COORDINATES: protein motif:HMM:NF015866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HOK60059.1"
                     /translation="MFTLEVCANSVESAINAQKGGANRIELCENLYVGGTTPSFGCIA
                     ETLNQLNIPVHVLIRPRPGDFVYSEMEFRQMLRDIEACKKLGVKGVVSGILRANGTLD
                     KERTKELVWAAKPMTFTFHRAFDIMPEPFKALNEIIECGCNFLLTSGQKNKAVDGLDL
                     IRQLIAEGGNKIALIAGGGINHSNILELAQNGVNQFHMSGSEIVARYNTNHTGLSFVS
                     ALLPENTVQQTNAETIASTVAKLKQYFNKK"
     gene            102595..103467
                     /locus_tag="PK049_00475"
     CDS             102595..103467
                     /locus_tag="PK049_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HOK60060.1"
                     /translation="MTREELVKHAKLFQREEITGYQVYKNLAKSTNDKNNKTVLAKIA
                     GEELKHYNIFKQISGVDVAPQKTVVWFFTICGKILGLTFALRLMERQEAKIQDVYERV
                     IPYMPEAEAVKKSEEEHEDQLIALIQEERLDYAGSMVLGLNDALVELTGALAGFSLAM
                     QNTRLIAVAGLITGIAASLSMAASQYLSVKTENDDRHAGKSAIYTGLAYIFTVAILIS
                     PFLLLTSYMLALATTLLLAIAIIFAFNFYIAVAKNLNFKHRFLEMVVISLGVSALTFG
                     IGLLVRNYLGIPID"
     gene            103485..104003
                     /locus_tag="PK049_00480"
     CDS             103485..104003
                     /locus_tag="PK049_00480"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOK60061.1"
                     /translation="MEPKNYKFISPFELNENTFDLLDNQWMLILAGSKDKHNSMTASW
                     GGFGILWNKPVAFVFIRPQRYTLQFVEQNDYFTLNFFEEKWRSALELCGSKSGRDIDK
                     TKAAGLTIAESLKAIAFQEARLVIECRKLYIDDIKPENFLDKEIISKHYPKSDFHRLF
                     IAEITSVFKKEG"
     gene            complement(104062..104487)
                     /locus_tag="PK049_00485"
     CDS             complement(104062..104487)
                     /locus_tag="PK049_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOK60062.1"
                     /translation="MRVDKWLWFVRVFKTRTLATEYCRKGRVTRNGIEVKPSQDINVG
                     DTLKVRKPPVTYTFRVKDLPKNRLGAKLVESYMENLTPPEELQKLDPSFMAFNIHRER
                     GTGRPTKKERREIDELFDSGFGGFDWDSFEPDADDESNE"
     gene            complement(104498..105061)
                     /gene="pth"
                     /locus_tag="PK049_00490"
     CDS             complement(104498..105061)
                     /gene="pth"
                     /locus_tag="PK049_00490"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOK60063.1"
                     /translation="MKYLITGLGNIGSEYSNTRHNVGFMVLDALAKASNITFADARYG
                     FVAQLKHKGRTFVLLKPSTYMNLSGKAVNYWLQKENIPLENLLVVVDDIALPLGAIRL
                     RPKGSDGGHNGLRNINDVLGTQDYARLRFGIGNGFSRGRQVDYVLGEWTDEELKVLPE
                     RLTLAGEAVLAFGTIGIERSMNLYNSR"
     gene            complement(105073..105648)
                     /locus_tag="PK049_00495"
     CDS             complement(105073..105648)
                     /locus_tag="PK049_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HOK60064.1"
                     /translation="MKTIELSAAPRTETGKKAAKHSRKSELVPAVIYGAGENIMISLG
                     EKELKKVIYTPIVYLVKLNVDGKMHDAIIKEVQYHPVTDKILHVDFLKVSENKPITIG
                     LPVNLVGQAEGVKAGGKLLQVVRKIRVKGLAKDLPDAISIDVTHLGIGKSIMVAELSF
                     DKFTVVEPKSMVIATIKSTRAAREAQQEQAK"
     gene            complement(105778..106722)
                     /locus_tag="PK049_00500"
     CDS             complement(105778..106722)
                     /locus_tag="PK049_00500"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOK60065.1"
                     /translation="MHSKHQIKFFTGRSSRYLAERIAQSFGIPLGKSTLTVFSDGEFQ
                     PSFDESVRGCTVFIIQSTFPPVDNLFELLLMIDAAHRASAYKVVAVMPYFGWARQDRK
                     DKPRVAIGAKLVANMLVAAGADRVMTMDLHADQIQGFFDVPVDHLYASSIFVPYIKSL
                     NLENIAIAAPDMGGAKRANAYSRFLNSSLAVCHKNREKANQVGEMTVIGDIEGKHVII
                     LDDMVDTAGTLTMAADLMMGKGAASVRAFATHPVLSGSAYERIRDSKLTELVVTDTIP
                     LRTDKDTSKIKVLSVAELFADVINKVYNYQSISEKFII"
     gene            complement(106778..107395)
                     /gene="yihA"
                     /locus_tag="PK049_00505"
     CDS             complement(106778..107395)
                     /gene="yihA"
                     /locus_tag="PK049_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOK60066.1"
                     /translation="MVIRTAKFVASYPSVQKCPKTDLPEYAFIGRSNVGKSSLINMLT
                     CTKALAKVSQTPGKTQLINYYLINDSWHLVDLPGYGYAKVSKGEREKFSDIVLNYILK
                     RENMHLLFVLIDSRLEPQDIDIQFINWLGVNQVPFALVFTKTDKVSSMVLEQNIELHK
                     KVLHESWEELPPIFTTSCVTRTGREDVLNYIDQVFREAKITHCQV"
     gene            107809..108987
                     /locus_tag="PK049_00510"
     CDS             107809..108987
                     /locus_tag="PK049_00510"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOK60067.1"
                     /translation="MTELKRVFPGFVIQNSVEGYLHSISKRSKKIYWLIIMIIIGTLA
                     SMPFILVDVTIRTSGLIKPSIEKQNIVLPYGGKVIYTKLKNNLRVSAGDTILVIDTIP
                     ISSKIKTLEFNLGELKSYQNDLGLLIEHNFLLQKFNPKSLVTSKYMLEVSSFQRQIST
                     QSNRIEKLKKEHSRNTLLYKQKIIAEVDYETSKYNLDNEILLRNQFISQQLASWQASL
                     AEITNQIPKIESEIETAYNELSKHFITATLSGTIQQCNELQVGSIIYANQIIAELSPD
                     CELIAVCYVQPSDIGFIRVNQDVKIQVDAFNYNQWGLLPATIKEVSDDIVSDGQNNMY
                     FIVKCKLSKNYLTLQNGFKGYIKKGMTISSRIIVTERSVYDLLFDKVDRWFNPYTEMK
                     "
     gene            108993..111152
                     /locus_tag="PK049_00515"
     CDS             108993..111152
                     /locus_tag="PK049_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004344797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HOK60068.1"
                     /translation="MRVKQRDITDCGAACLASVAAHYKLNLPIAKIRQFAGTDRKGTN
                     VLGMIEAAQKMGFHAKGVRGDFDSLFKIPKPSIAHVVVKEVLQHFVVITKTTKKYIEV
                     MDPGDGRFHKIPHEEFKKSWTGVLILLVPGETFKPKSEKVSSSFRFWNLIKPYKGVLT
                     QAIIGAVLYAVLGLSTSIYVGKIVDYAIPSNNANLLNLLGIAMLIIIGVRFLLNIFQS
                     VFVLKIGQRIDATLILGYYQHLLRLPQSFFDTMRVGEIISRINDAVKIRLFVNEVTVN
                     LIVNVLILIASFMLMFTFYWKLAVIMLLVMPLYIITYMIINRLNRKVQRSIMENSAEL
                     ESHLVESLNSISTIKRFGLEGYSNLKTEMRFVNLLKPLYKSGINSIYSNNVSGLISQV
                     ITIVLLWVGAGFVLESTITPGELLSFYSLIGYLTSPVTSIIGFNKTYQDAKIASDRLF
                     EIFDLEVEDEKNKIEFTAEHVGDITFKNVDFRYGTRVEVFKALNLHIPKGKVTAVVGE
                     SGSGKTTLTSLLQNIYPLQSGNIYIGKFDLRYFTNESLRDAVVAVPQNIDLFAGTIVE
                     NIAVGEFNPNIQRIIEICTQLGMIEFIESLPNRFNTYIGEHGTSLSGGQKQRIAIARA
                     LYKNPEILILDEATSSLDSIAESFVQHTVELMRQKGKTVIVIAHRLSTIAMADNIIVL
                     DNGKVVEQGVFKELIHSNGQFCRMWQHQTVEKHIIEN"
     gene            111160..111534
                     /locus_tag="PK049_00520"
     CDS             111160..111534
                     /locus_tag="PK049_00520"
                     /inference="COORDINATES: protein motif:HMM:NF019884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HOK60069.1"
                     /translation="MKFFEYLVRIEKMHCLIKEQRTGTPDEFAAKLGISRTRLYEVID
                     ELKSRGAPIVYSKSRETFFYEYSFDISLKCTMKPLSRKELVEKNGGASLPFLFSGRNA
                     PNFATVTLQKHNQIWMAKLLTF"
     gene            111547..111732
                     /locus_tag="PK049_00525"
     CDS             111547..111732
                     /locus_tag="PK049_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60070.1"
                     /translation="MEQLSLNKFGVQQLNAKEIVEIEGGNPLLWLLAGVIVSELLDRN
                     APADFWEGWNDAKQNKK"
     gene            111739..111966
                     /locus_tag="PK049_00530"
     CDS             111739..111966
                     /locus_tag="PK049_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60071.1"
                     /translation="MNTQKNPTLQKIKAREMQYLEPLNATDMLNINGGTWLSYWLGYI
                     LSSIDNNVPNEDLTRYGGAYLETISGRSSIM"
     gene            112454..112699
                     /locus_tag="PK049_00535"
     CDS             112454..112699
                     /locus_tag="PK049_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60072.1"
                     /translation="MGTYEFTTLLGISRTRLYEVIDELKSNGAPIAYSKTSTTFYYEY
                     PFDILVGCTMKPLSRKELVEKKWRCFTSVLVFRTEHS"
     gene            112786..112986
                     /locus_tag="PK049_00540"
     CDS             112786..112986
                     /locus_tag="PK049_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60073.1"
                     /translation="MELEKFGVVEINATEQQDENGGFLYTLAGGLISNAIGVAACVGV
                     VVVAGYAIYAGFEYGYQVVQNS"
     gene            112992..113168
                     /locus_tag="PK049_00545"
     CDS             112992..113168
                     /locus_tag="PK049_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60074.1"
                     /translation="MENLNNIGMSEISMEESLEINGGNPIGVLIAIGGVYTGLFAIGF
                     AAGVIYGLVEKATE"
BASE COUNT        34445 a        24628 c        23364 g        31271 t
ORIGIN      
        1 agttcatcaa atcaaccgaa gaactgagtt tagggtgctt agggaagatt atcaaggaaa
       61 gtaatatttc aaccccggtt tctaccgggg tttattttcc ctaggtaaac acgctggcac
      121 taaatttgtc atcactttat tacttttcaa acattttgtt agttttgcat tgaaccagct
      181 attcaatgta acctaatgaa agcattatta gtataatgat tttgttatac aatgcgactt
      241 tcaataaaaa tattatccat actatttgtt ctgcttggct tcagtggcat atcagcagct
      301 caaaagtttt atgaagctga agatgtcaag gatgcccagg tgaaattctt tattgttgat
      361 agtccagagc aggcagacct gcatttctgc atcatatacg aggcttccga aataactaaa
      421 ccgggtgtaa tgatggaggt tgaaaccccc aaggaggccg atatcactct gatatttgtt
      481 gataatgaga aagatgccga tattaaggtt tggcttgtag acactccagc cgaggtgaaa
      541 tggattaatg agtcgaaaaa gaaattgctt tctgttgggg gattaaagta attaccctct
      601 ttcatcttca agttggttgt aacgtaaata taggttacct ttgcagttaa aacgcacccc
      661 atgaacaaat attcccgtta cgaccaacgc ggcgtttccg catcgaaaga tgatgtccat
      721 aaggcaatta gcaacctgga caaggggctc ttcccaaaag cattctgcaa ggttgttccc
      781 gattatctta caggcgaccc cgattactgc ttggtgatgc acgccgacgg ggccggtacc
      841 aaatcgtcgc ttgcctatgt atattggcgc gaaaccggcg atatgtccgt ttggaaaggc
      901 attgcacagg acgctattgt aatgaacatc gacgatttgc tttgcgtggg gattaccgat
      961 aatattttgc tatcatcaac cattggccgt aacaagaacc tgattccggg cgaagtggtt
     1021 tcggccatca ttgatggcac ggaggagttc tgccaaacca tgcgcagcat gggaattaac
     1081 attgttctta ccggtggcga aacagccgat gtgggggatt tagtgcgaac agttattgta
     1141 gattctactg ttactgcccg cataaaaagg agccaggtta tttccaatag caatattcag
     1201 gatggcgatg taatcattgg tctggcatcg ttcggccaag cctcatacga aaaggaatat
     1261 aacggaggga tgggtagcaa tggactaacc tcggcacgcc acgatgtgtt cgggcactac
     1321 cttgcccaaa agtatccgga aagctatgac cctcaggtac ctgaaagttt ggtttacagc
     1381 ggcaacctta agcttgagca acccgttgag ggaactggcc ttacggcagg caaactggtt
     1441 ctatccccta cccgaaccta tgcacctatc attaaagcca tactagataa ttaccggcct
     1501 gtaatacatg gaatggttca ctgttcggga ggcgcacaaa ctaaggtgat gaattttgtg
     1561 aacaaccttc acgtggttaa ggataacctc ttccctgtac caccgctatt ccgtataatt
     1621 caagaacagt caaatactcc ctggcaggaa atgtataaag tattcaacat ggggcaccga
     1681 ttcgagattt atgtttaccc tgagtttgcc gatgacatta ttgcaattgc taaagagttt
     1741 aaggtagatg cccaaattgt tggatactgc actgaggcta cttcaaataa attaaccatt
     1801 aagtcgcctt acggtgagtt tcattactaa ttcaaaaaga gattttttgc ttacctttgc
     1861 gccaataaat ttttgatatg gcagataata ctatactgcg taagcttgaa ggaataaggc
     1921 ataagttcga ggagattggc cagcagataa ccgatcctgc tgtaatggcc gacatgaaaa
     1981 agtttgtcgc acttaacaag gaatacaggc agttggagcc cattgttgag gcttacgaaa
     2041 aatacaaaaa cgttctgagc aacatagaat cgtccaggca aatactggct aacgagaagg
     2101 acgaggaact ccgtgagatg gctaagatgg agctagaaac ccttacccag gaacaagagc
     2161 aactcgagga ggaaatcaaa attctacttc tccccgccga tcctcaagac tccaaaaatg
     2221 ctgtaatgga aattcgtgct ggcaccggtg gcgacgaggc aagtattttt gccggtgacc
     2281 tgttcaggat gtatacaaaa ttctttgaga agaaaggttg gaagtacgag gttaccagtt
     2341 tctccgaggg tacggttgga ggttataagg agattatcat gaatgttacc ggtaatggcg
     2401 tttacggtgt gcttaaatat gagtcggggg ttcatagggt tcagcgtgta cctcaaaccg
     2461 aaactcaggg tagagttcat acttccgctg ctaccgttgc cgtacttcct gaggccgatg
     2521 agtttgatat cgaccttaaa atggaagata tccgtaagga taccttctgt tccagtgggc
     2581 ctggcgggca aagcgttaac accacctact cagccattag attaacccat atacccaccg
     2641 gcattgtggt tcaatgccag gatcaaaaat cacagataaa gaattttgaa aaagccctta
     2701 cggaactccg tacccgcctt tacaacatgg aataccaaaa atacctcgat gaaattgcat
     2761 cgaagcgtaa aacgatggtt tccactggcg accgttccgc aaaaatcaga acatacaact
     2821 acccgcaagg gcgtgtaacc gatcatcgaa taaatctcac cctgtataac ctgtcggcca
     2881 taatggatgg cgatttacag gaagtaatag ataaactaca gcttgccgaa aatgctgaaa
     2941 ggttaaaagc agctgctgct gaagaataaa aaacaatgcc tatgacagcc caagaactat
     3001 ttgaacagat aaaaaagaaa cgaagtttcc tgtgtgttgg gctcgatact gaactcacta
     3061 aaatccctga gcatttgctc accaaggatg atccaatgtt tgagttcaac cgtaaaatta
     3121 ttgaggccac ccacgattta gccatcgcat acaagttaaa tcttgctttc tatgaggttc
     3181 atggtgcagc gggctggaat agcttggtga aaaccgtaaa ataccttaaa acgcatcacc
     3241 ccgatgttct ggtaattgcc gatgctaagc ggggcgacat tgcaaacaca gccagaatgt
     3301 atgctaaagc gtactttcat agcttaggct gcgatgccgt tacagtatcg ccctatcttg
     3361 gccaggactc ggttgtcccc tacctacagt acgacggcaa atggacaatc cttttggcaa
     3421 tcacatcaaa cgagagcttt ggcgattttc agctggtaca caataagcaa accggcaacc
     3481 tcctctttga ggatgtagtg cttaaatcga tgcactgggg cagcgatgcc aatatgatgt
     3541 ttgttgttgg agcaactcac cctgaatact ttgctgctgt tcgtaagcat gcacctcacc
     3601 acttcctgct agttcctggg gttggcgagc aaggcggaag ccttcaaaaa gtagcagaaa
     3661 acggcatgaa cgaccgttgc ggcctgattg taaattcatc gcggggaatc atttacgccg
     3721 atattaccgg gaattttacc tctgaggctc gtaagcgcgc ccttaaactc cagcaggaaa
     3781 tggaagtgtt gctcaaacag aatggattaa tatagttcca cttactgctc tataaccttt
     3841 ttaccaaacc acgaccaaaa attaagatga aatttctcgc cagcttcacc ggagtacata
     3901 tttaagtatg taagcctcga tggctggttg ggcaaaaagc gtaagaactc atcgttatta
     3961 tataggagtg taggctctgg atggaactgc agtccaatta cgtttgggta ttttgtatga
     4021 acaatagcct ctaccacatt accatcaatt gaggtggcaa taatttttaa attccttccc
     4081 agtcgttcca ccccctgatg gtgactgctt aatacccagg gctgtccctg gcaaatggat
     4141 tttagaggtt catcaaggat actgatctgg tggaaattcc cccaaaggag tttatcgtcc
     4201 aaagcataat tggcattgta attcctgtgc aagctgttgg tttgctgtat gagggcatcc
     4261 tcaacacttt caattccgta aatctcggaa gggatatcct gcactaaagt gccacctgct
     4321 gcaacgttca tggtttgcat tccaaggcaa atgcctagga caggcattaa aggcttggca
     4381 tcgagcaggg gctgaaataa agtatccttt gagcccccaa ttaaatgaaa catgaacgac
     4441 acctcaaagt aatggcgagc cggatcggtt atttgggtta atagcgatgt tttctctcca
     4501 taaattgatg gggggatatc ggggccacct gtaaagatta caccatcgct caccttggtt
     4561 aaccgagcaa aaacatcagt acattcgttt acccggtaaa ttgcggtttc ggttattggt
     4621 tccttaacgg gcaacagctt aaaattctcc agcttattct tcttaatgta ctcaaccgat
     4681 tttgagtaat cgtatttttc agaggtgtga tacacaccaa taacttttgt caccgaaggt
     4741 attggcaata tcttgctatc aattaaatag ttaaatgttt tgatattgtt ctcggttggg
     4801 tgtactagaa ttagtaccct atcgtgtttc ccaatgtcat caatccctat aacaatatta
     4861 ccagaatctc tactacagga taggatgcct agcaatactc ctgtagtaat cagtaaaact
     4921 ttccatctgt tcttcataac tatagtatta aggtactaaa acctaaaacc catgttaaac
     4981 ataagtgtac tgggaaaaac atttctatcc ttttctaatg acaacactgc aacctgacga
     5041 acaaactgga aattaacatt cataatgctg aaccccacgc caacctgaaa gccataatta
     5101 tccttttgaa tgttattgtc catgtcaaaa cttccattac caacctttcc ggcaaatcgg
     5161 taggagtagt atcctcctcc tataaagtac agatttggcg ataacatttg cttacttttc
     5221 ccacccatgt aaagaaggat actggctgga gccataacct catgggttct gaaatgacca
     5281 taccgatgtt ctgttccagt tgtttggtaa agtgcaccaa cctgtaaatc gatataccta
     5341 cttaatccaa ttgtgaagga taaccctgtg ttaaaagaaa aaccttcctt tgagttgtaa
     5401 aactcatccc tgtaatcgtg ataagtatta ccaatgccta cttggtatcc agccgaaaat
     5461 ttatcgctac ccaaagtttt tgcagcaaac cttgcatcgg gtttaacgct atcagtagtt
     5521 gaaagctgta gggttgcatc ggctacaaaa cttgcaattt tcgccattcc cacatagtca
     5581 agcttatcgg catcgtcttc gggtttatgg taaggcgaaa ccgtagaggt aaacacatgc
     5641 acggcaggta ttcctatttt cccaaacggt tgagtgtcgg tttgcatttc aacacttcgt
     5701 cccgattttt tcacttgaat accatgcttt gaagccaact gaacgaaaaa ttcatcgcca
     5761 tcgctaagcg ttttatttcc aaccaggtcg atacctccat acttttgcag cattccaacc
     5821 atgtcgaggc taaacataaa ctttaccctg tcggtaggga taaccttatt gtttacaagg
     5881 tgacttgaac ctattaatcc ggcctcctcc ccatcgaaag caatgattat aacagagcgt
     5941 ttaagcttat cgcggttaag tacaagccag cgcccaattt cgataattga agcaaccccc
     6001 gaggcgttat cgtcagctcc attgtaaacc actttttcgc cgtttactat tttataggcc
     6061 acatggtcgt aatgcgctcc aagtactatg tattcatttt ttagtaccgg atcattcccc
     6121 tcaacccaac caataatatt tgccccttca gtcttgagca tcatactgga actgataaat
     6181 gagtgtaggt actgcccttg ccaaggagtt agtccaacct ctttgaactt atcagtgatg
     6241 tattttgcag ccatgcgccc cccttcagtc ccaaaacccc tacccataag tgaatcggac
     6301 gcaagatagt aaacatgatc ctttaacata ttcgaatcaa cttgctgagc aaatgataaa
     6361 acgctgcaat aaaaggtagc aagtgcgctt gcaataattg taatcctatt cattttacaa
     6421 gacctttgaa aatttattgt aatgttacaa aatattttaa aatgctgacc ctataaagca
     6481 gtattattaa cctaaaagtt tactctagtc aatagatatg cttttaacat ttttttatat
     6541 tttttttaac ttgattatca atactttaca atatttttta tagtttagat aaaaaaaaat
     6601 acgcgaatac gaaacaatga attgaacaac cccatacatt tgcattgaaa attaaacttt
     6661 aatgtttaac taacaaaccc aaacatttcg attatgaaaa agacactttt acttggtttt
     6721 gcagccttaa ctatggttgc tatgctttca agctgtggca aagtgcccca ggaaaagatt
     6781 gatgctgcta acgccgctat tgattcggct aaagctgctg aagctgatgt ttatcttgct
     6841 gctgatttcg ctgctgttca ggattcaatg agcgctgtta tggctgacat tgaagttcaa
     6901 aagtcaaaat tattcaagaa atttggccca gctagcgaaa aactcgatcg tattcttggc
     6961 atggctcagc agctaaaggt taacgctgtt gctaagaaag aagaagttaa gaaagaggta
     7021 gaaggcctta tgaccgaagt tgccaccatg atggaagaaa atggcaagct catgaagaag
     7081 gctcctcgtg gtaaggaagg tgctgctgtt cttgagcaaa tgaaagctga aatggctacc
     7141 atcgaagcta ctgttaacga agctaagggt ctttacgatg ctggcaagta catggatgct
     7201 tccaacaaga tgaaagctgc taaagaaagc gctaccaaga ttaacaccga acttaacgag
     7261 gctattgcta aggttaaggg cagaaagtaa tttatttaca tgctataaaa tccccggccg
     7321 tttttttacc ggggattttt ttttgatatt tgtacccgta tgaaaccgat tttaaagtac
     7381 agcttaatta cccttgcaac gctatcagtt ataggtggcg gatttgcact attttactat
     7441 tccatacccg aaccgcctaa aggcgatatc gattatgcct tagcctcgct acaccaggca
     7501 tcgcggtcaa atgccaaacg ttactcaaaa caaaagtacc taaaggcaaa ggcatacttt
     7561 gattcggcca tgaagcattg gaatatccaa aatgaacgct tcattttaag ccgcgactac
     7621 cagaaggtat cgtactttgc caagaaatcg gccgaaatgg ctaaaagcgc aacaagtaac
     7681 tccaaggaac tttcaaaaaa cttggttata tcggttaagg ataaaattga caacctaacc
     7741 gcccttgtct cgaacatgaa caatcttttt cagaggttac ccctgcctga tgagtttcgc
     7801 aaaaaaatct caaaaggtaa aatgttaatc catgaggcag aggtagcatt taagaaaggc
     7861 gattaccttg agtcgtcgga aaaaatcaag ttggctgagg atttaattat ggattcctac
     7921 aaaaaaaccc tcgacgacct tgaagattac tttaactcac aaccctactg gaaccgactt
     7981 gtaaaaagcg ctattagcaa tacagccaaa aacaactcca tggctattat tgtggataaa
     8041 tttgcccgaa aactctatat ctaccaaaac ggtacaaaaa agtatgaatt tgatgccgag
     8101 tttggcaaga attggatggg gcgtaaacgg ctgaaaggcg acaaggctac tcccgaggga
     8161 atttatctgg ttaaaagcaa aatacctgca aataaaacca agtattttag ggcgttgcta
     8221 ctcaactacc ctaattcaga ggatattgaa aatttcaaca aggaaaaagc ttcaggccga
     8281 atccccaagc acgctcaaat tggcggacta attgagatcc atggcgatgg cggcaaagga
     8341 accgattgga ctgatggatg tattgctctt gacaacaacg atatgctaca ggtgtataag
     8401 attgttgaaa ttggcactcc ggtagttatt gttggatcaa ttgccgactt caacaccata
     8461 gccatgagcc tgaaccttaa acgtaatcaa ataaagggtt aactatgaat agcaatgaag
     8521 ataaaataga tttcaacccc gaaataaacc ctaagctaaa acggttgggc tttgtaatgc
     8581 tgcttatcaa tatcatcctt tcaggcttca tcattcttta tttttatctc acctatcttt
     8641 cgcaacccat ggcggaatta gtggttaaga acagaaagtt aacctttgta tccgactcca
     8701 tacaggacga taaggttgat gtaaagaaac ttcaaaagga agtggcctca ttaaagcaac
     8761 agttccaaaa ccttacgccc gcacaaccat acattgtggt taacacaacg tacaatcgct
     8821 tcaggctata caaaaaccgc aaacaggttc gtgaaggatt ttgtagtact ggcagctata
     8881 cactactaaa gtcgcacgat aaacgtcaat ggatttttca taccccaaag ggtatgtata
     8941 aggtgttagg gaaggtaaca aatcctgtat ggcgtcgccc ggactggtca tacgttgagg
     9001 aagggttacc cattccatcg gccaacgacc catcgcgttg ggaagctggc gttttgggcg
     9061 actatgccct gagcattggc gatgggtacc tgatccatgg aacaatatac aagcgattcc
     9121 tggggatgcc tgtaacgcac ggatgtatca gaatgaatga cgaggatctt gaagcagttt
     9181 acaaaaccct agatctggga tcgaaggttt acatttacta atgatcgttt ttatgcgaaa
     9241 acctataaaa atcaccctct acaccacagc catactagtt gtaatactgg ctgttgcata
     9301 taccattctt aaagtaatag cattccagaa agccataaac aacattgagc aacacattgc
     9361 aaaagagctc aataaacgaa agaaaacagt tgaatgggtt agtgaacccg actcaacttt
     9421 aaaaaaatta ttggtggata aggcttacta caacgcattg attaccgctt ccaaaactga
     9481 ctcaatattt ttagccatta atattccgga cagtaccgtt tcgttaatga ttaaaggggt
     9541 tgttgctcat aaagcaaaag cagagcatat taaagtaagt cctttgtttt ctgctattaa
     9601 tccacccttg cttattcaag gattgtcggt tccattaaca gtatcgaagc atacctcaac
     9661 catcccaaaa gtaccattaa tgatcaaaat tgccccacgc gacacctcag agtacacgcc
     9721 cgatgttata cctgatacaa ccgaccttga ccctgttaac tttattattg agctaaaacc
     9781 aaacatttta ctttacttct acgagtcaaa cccttcgcaa tcgtcggatg ggtttaatca
     9841 gttcaagttc gatcttagag agcgcacgtg gactgttgtt caggatgtta aacgaatgct
     9901 aaaacttaaa acaccggttt acaagcccta catcagattt aagcttggca agaaagatgc
     9961 aaaggtgata taccgggcaa ttcccaacca gggacaagta gttttacgat tagaataaaa
    10021 aaagagctga cattaaatca actctaaagt cggggtgggg agactcgaac tcccggcctc
    10081 tacgtcccga acgtagcgcg ctagccaact gcgccacacc ccgtttgcgc ttgcaaaatt
    10141 actacaattt tactttcgcc ataatagtat tgcccagttt tttggatttt tttaacttgt
    10201 acatccaaat acaattcacc ctttttaaat ggacgaatcg cttttacact tccagtggcg
    10261 ctaccagcac tataccctaa atgggctcag cactacaaaa ggcgatacgg ttgaggttat
    10321 cgatcccgga ttgtacaata ccgactcagg ccccgatttt tccaacgcaa aagttcgaat
    10381 tggtgatact ctttgggtag gtaatgttga aatgcacatc aaatcgtccg actggtttac
    10441 ccataaccat caaaccgaca acgcttacaa caatgtaatt ctacacgtag ttgttgagca
    10501 cgatagaacc gcagtaacat cgaaaggaac agaagttcag acactagtca ttccttaccc
    10561 caacaaactg gtgcatagcc atgaaaactt gatcaaatcg accgaatgga tcccatgcgc
    10621 cagtaaaatt ggggttgttg attcattgct aattaactca accattgatc gaatgcaagt
    10681 tgaaaggcta gaaaacaaga gtaaacgtgt agctgagctg gtaagccaat gcaatggaag
    10741 ttgggaggag gctttctatc gcgatttatg ccgttcattt gggctaaaaa gtaacgcact
    10801 tccctttcag ctactggctc aaagcttacc gcaaaaagta ttggcaaaac ataaaaacag
    10861 tgcatttcag cttgaggccc tactattcgg tcaatcgggc ttgctcgata aagcaaaaaa
    10921 cgatagctat gtaatttctt tgaaacagga gtatacctat ctgaaaaaca aattctcact
    10981 tacacccata gagccaagcc tctggaagtt tatgaggctc aggccagcat cgttcccaac
    11041 cattagaatt gctcttttgg caatgcttgc acacaaatcg tcgggtctct tctctcagct
    11101 tatggaggtg aaaaatattg ataatgtaat taaattcttt gctgttgaag catcggatta
    11161 ctggactaat cactacgttt ttggtgagga atcaatttcc aagaaaaaac gtttgggcga
    11221 ggaaaccatt aggttaataa ccataaacac aattatccct ttcatgtata gctatggtcg
    11281 gagcagggca aaagctgacc ttgaggccaa cgcactaact atactccaac aaatgccacc
    11341 cgagaataat acaattgtca gaaactttgg tgccttgggc atcaaagcat tgtcggcttt
    11401 tgactctcag gcattggttc acctaaaaac cttttactgc gagacccgaa aatgtattta
    11461 ctgccctatt ggtgttagta ttttacgaaa acaattataa ttttaaagcg tcgtaaaaat
    11521 tgctgaatgg agcaaaaagg aggtagaaca ttagcgcttg caatcacaat tctcctaagt
    11581 ataataacca taggaacagt tggttatata cacattgaag actttacatt cactgaagca
    11641 atctacatga ccgtaattac aatcggtaca gtaggattta aggaggttcg gccgctttca
    11701 ccagagggaa tgtggtttac cataacacta atcatcataa gcattggatt ctttgcctat
    11761 gcagctacct tcttagccaa atacttttcg gaacaaatac tttcccgata ctcaaataag
    11821 aagatcatgg agaagatgat tgccaaaatg caaaaccatg ttatagtggt gggatacgga
    11881 cgtaatggtt cacaggcagt tgaagaactg cttgcgcata acatccctgt tgttgttatt
    11941 gagaacaggc ccaaagtaat tgagtatata cagcaaaacc ccaaaatagc atatgttgat
    12001 ggcgatgcca cccttgatat caccttgcag aaagcaggag tggataaagc taaagcgcta
    12061 attacggctc tggcgaacga tgccgacaac ctttttgtag taataaccgc cagggaaatg
    12121 aatccaaagc ttaaaatcat tagcagagca tcagccgtta caaacgatag aaaattaaag
    12181 cttgctggcg ccgacaatgt gatactgccc gacaaggttg gaggacaaaa aatggcaaaa
    12241 ctggttattc aaccggatat tcttgagttt gttgagaata tcatgctaaa acgaaccggg
    12301 aacattagcc tttttgaaat atcatgtagg aaaattctcg aggttggggc aaaatcgttg
    12361 gatgaactac aaataaggga aaaaacaggc gttaatgtaa ttggcgttag gaatcccatt
    12421 ggcgaataca ttttaaaccc tgaacctaat cacatactta cccacgactc tttactgttt
    12481 gttctgggaa ctacagagca agttgaaaag ctaaaagatc taatctccta aaacatttta
    12541 aaatgaaaat ccttgttact ggggcaaatg gccttgtagg gaaaaacata gcaaaagctt
    12601 tacatgccac taaccaggtt gaagtttttg ccacatcgct caaaaagttt cagctcgagg
    12661 gggtaaccgc ttttacatcg aacctgttga atgctgacat caaccacatg gttgatgata
    12721 ttaaacccga gattgttgtt cactgtgctg ccctttccag cccggatgcc tgtgaagttg
    12781 acaggtacgc ctgtcacagg atgaacgttg agctaaccaa acgcattgta tcggcctgcg
    12841 ttgactataa tgttcacctt gtgttcctct caaccgattt catttttgac ggctctaaag
    12901 gcaactacac agagatggat gcacctaacc cagtaaatta ctatggggaa tcgaaacttg
    12961 aagcagaaaa gttaatacag gcctcgggtg tggtcaatac aatagtaaga acctctttgg
    13021 tctacggggt tgagtccaaa ctttcgcgtc caaacattat tacccgtgta ttcgataacc
    13081 tcagcaaaaa caagcagtac aaagtaccaa gcgatcaaat ccgcacccca acctatgctc
    13141 cggatttagc ttctgccatc tcccagatct gtttgaacag ggttacaggc atttataata
    13201 tttctggcga ccaaataatt tcagttgctg attttgccat acttgctgca aaagcctttg
    13261 acctgaacca tgaattgctg ctgcaagtaa attcaaaaga cttagccgag gctgctccca
    13321 gaccaaaaaa cacctcgctg gttataggca aggcaaaaaa cgatttaaac ttcaaaccca
    13381 ctcccattga aatggcactg ggaaaaatta aaactacgct aaatagttaa aatatactat
    13441 aaacatgtta atattctgta tgtcataata ttaagcaatt aaaaattaat agctgttcga
    13501 aaaaaaagta tcaattgtaa cgatattgaa aaaaaggttt tatctttgca cctcaaatta
    13561 agtagtaaat ctacccacta attatcagtg ttatgaacta tttgacggcc gaacagaaaa
    13621 aagagatttt tgagaagtac gggaagtcca ataccgatac cggctcacct gagagccaga
    13681 tcgcgttatt ctcctaccgt atcaaccatt taactgagca cctaaaggtt caccgcaagg
    13741 attatggtac ccagcgcgct ctgttaaagt tggtgggtaa gcgcaggagg cttttggatt
    13801 acttaaaaga aaacgatatt gaaagatatc gaacaatagt aaaggaatta aatctcagaa
    13861 agtaagaaat caaaaaaggc aatttctaaa attgcctttt tttaatcact atcataacct
    13921 ccagaggatg ggaatccttt ggggtttaat tgtttaaaca aaggcagtat tttattgttg
    13981 attttatcta tataaatttt aattatgcag aaagttgtaa aaaagacaat cgatttgggc
    14041 gatggccgaa ccattaccat cgaaaccggt aaactggcca aacaagccca tggctcggtt
    14101 gtgctaacaa tgggtaaaac catgcttctc gctacagttg taagcgctca ggaagcaaaa
    14161 gaagatgttg atttcatgcc gcttagcgtg gagtacaagg agaaatttgc tgcaagcggc
    14221 cgtttcccag gaggtttctt aaaacgcgaa tcaaaaccat cggactatga aattctggtg
    14281 gcccgtttgg ttgaccgtgc attacgtccc cttttccctg acgattacca tgccgaaacc
    14341 tttgttaccg ttaacctgat ttccgctgag aaggaaatta tgcccgacgc acttgccgga
    14401 ttggcagcat cggctgcgct tgctgtttcc gacgttcctt tccatggccc aatctcagag
    14461 gttagagtgg ctcgcattaa cggtaagcta aaaattaacc ctacctttaa ggaagttgag
    14521 gaggccgata tcgatatgat tgttggcgcc acctacgata atattctgat ggttgaaggc
    14581 gaactgaaag aggtgtcgga agccgatatg ctggaagcaa ttaagtttgc ccatgaggcc
    14641 attaaacccc agtgtttggc tcaaatggag ctcgccaagg aacttggcgt agttaaacgc
    14701 acctactgcc atgaaaccaa cgatgaggaa ctcaaggcaa aggttcatga gttttgctac
    14761 gacaaggttt accaggtagc caaatcgatg ctgcccaagc aagaacgcac ggcagctttt
    14821 gaagccatta agcaagcctt tattgaaacc attccggaag aggagcgcga agcaaagcag
    14881 ttcatggtga accgctacta ccatgcggta gaaaaagaag ccatgcgcaa catgatactt
    14941 aacgagggtg ttcgcctcga tggtcgcaaa acccacgaaa tacgccctat ctggtgcgag
    15001 gttgattatt tgcccatggc acatggatca gccattttca cccgtggcga aacacaatcg
    15061 cttaccactg taacccttgg taccaagctc gacgaaaaaa ttattgatga ggttctgatg
    15121 cagggaactg agaagtttac actgcactac aacttcccac ctttctccac tggcgaagcc
    15181 aaaccctcaa gaggtgtgag ccgcagggaa attggacacg gaaatcttgc ccacagggcg
    15241 ttaaagaaca tggtgcccga tgctccagaa aacccatacg caattcgtgt tgtgagcgat
    15301 atacttgaat cgaatggttc atcatcaatg gccactgtct gcgcaggcac cctggcgctg
    15361 atggatgcag gagttcagat taagaaacca gtatcgggta tagcaatggg tcttatcact
    15421 gagaaaggat cggataggta tgccgtactt tccgacattc ttggcgatga ggaccacctt
    15481 ggtgatatgg actttaaggt ggcaggaacc cgcgatggta ttacagccac ccaaatggat
    15541 ataaaggttg atggcctatc gtacgaagta cttgctaaag ctctaaagca agcccacgat
    15601 ggacgtatgc acatccttga tatcatgatg caaaccattt ccaaaccccg tcccgacttt
    15661 aaaccccacg ccccccgtat tgagcagttc aacatacctc gcgaaaccat tggtgccgtt
    15721 attggccctg gtggtaaggt aattcaggaa attcagcgcg aaacccaaac cactatcacc
    15781 attgaggaaa ttaatgatca cggtgtagtt agtgtatttg ccgagagtgc tgaaagccgc
    15841 gatgctgccg ttaaatggat taaaggaatt gttgctgttc ccgaggtagg cgagatttac
    15901 aagggtaagg ttaagtccat tatgcccttt ggcgcatttg ttgaaatact tcccggcaag
    15961 gatgggctgc ttcacatatc ggaaattgag tggcgtaggc tagagaaggt tgaagaggtg
    16021 cttaaggaag gcgatatggt tgatgttaag cttattgaaa tcgacaaaaa aaccggaaag
    16081 ctgaaactct cacgaaaagt tttgctgcca aaaccggaga aaaacaaata gtaaaagcag
    16141 ggtaacccct gcttttttta tactttcaaa tattgtgatt gctttttttc agaaattagt
    16201 gcaaggtttg cataatttta ttgtattttt gttagtatat caaaacaaaa ttttgaaagc
    16261 gatgaaaaag atattcatat tgatgttgtt agccacacca attttgatta caatgtcgtg
    16321 taacaccgca aaagttaaat atcccaaaac caagcaggta gatgtagttg acgagtattt
    16381 tggagtaaaa gtggccgatc cctaccgttg gcttgaggat gacaaagcac ccgaggtggc
    16441 tgaatgggtt aagcagcaga atgaggtgac tttcaactac cttaatgcaa ttcctttccg
    16501 caacaaaatt aaagaaaggc ttaccaccat ttggaactat acctcaatgg gggccccttt
    16561 taaagcaggc ggcaggtatt tctttttcaa gaaagaggga cttcagaacc aaagcgtcct
    16621 ttacatgatg gaaaaccttg aggctgagcc taaggtaatt cttgatccaa atacgtttag
    16681 tgaggatgga accgtatcgc tatcgaatgt tagcgtttcg cgcgatggca agtaccttgc
    16741 ctattccatt aacgatggcg gttcggactg gcaaaagata aaagtgatta aactgcccgg
    16801 cggggagcaa cttcctgacg aaatcagttg ggtgaaattt tcatccattg catggctaaa
    16861 cgatggcttc ttctatagcc gctacgacga acccaagggt ggctcggaac tttccaatat
    16921 caaccaaaac cataaagttt tttaccacaa ggtaggaaca ccagtggaaa ccgacaagct
    16981 aattttctca aatagccaat accccttacg aaactactcc gcacaaacca ccgatgacga
    17041 aaaatatctc atcatttatg aatcggaatc aacctacggt aattccatct atgtaaaaaa
    17101 cctaaaagac cccagggcaa acttttcaaa gattaccaca ggctttgagt ttgagtacag
    17161 cgttcttgac catgttgacg acaacctcat tgttttaacc aactacaagg ctcccaagta
    17221 taagctggta aaaatcaatg tgaacagcat ggatattggc aactggcgcg acattctacc
    17281 tgaacggaaa gatgtactga aggaatgtgc cctaatcaac aaaaaaataa ttgccaacta
    17341 cattgttgat gctaaaagcc aggccgaggt atacaacctg gacggaagta aaattacgga
    17401 cattgaactt ccaacccttg gcacaatatc agggataagc ggcgatataa atgattctat
    17461 tgccttttat tcattctcat cgtttacagt tcccccaacc gttgtaaagt acaacgtaaa
    17521 caccaatact tcgacacaat tctttagccc taacatcgac tttaagtttg atgactacga
    17581 aaccaagcag gtattttaca ctagcaagga tggcactctt gttccaatgt ttattgtcca
    17641 caaaaagggc attaagctcg acgggaataa ccctaccctg ctttacggct acggtggctt
    17701 caatgtaagc ctactaccct actttagcac atcgcgctta ttctggctag agcagggtgg
    17761 catatttgcc atggcaaacc taaggggcgg tggcgaatac ggtgagaact ggtatcgtgc
    17821 gggcacaaag ctaaacaagc aaaacgtttt cgacgacttt attgcagcag ccgagtactt
    17881 aatcaaagaa aagtacacct caccaaaaaa acttgccata caaggggggt caaacggtgg
    17941 cttgctgatt ggtgcagtaa ccaatcaacg tcccgattta ttcaaggtag ccatccctca
    18001 ggttggcgta atggatatgc ttaggtacca taactttacc ataggctggg cttgggctaa
    18061 cgattacggc accagtaacg actccattca gttccttaac ctttacagct actccccgct
    18121 ccataatatc aaacctaaaa ccaattaccc ggccatactc gtaaccactg ccgaccacga
    18181 cgatagggtt gtgcctgcgc attcgttcaa gtacattgct accatgcagg agaaaaaccc
    18241 taaccccaaa aatcctgccc tgatacgcat tcaaactcgt gccggacacg gagcaggtat
    18301 gccaacctct atggttatag atgagtacgc agatatttac gcgtttataa tgtataacat
    18361 gggaatgcaa tactagagtt gataaatttt taaaggggtt ggggtattac gctccaaccc
    18421 ttttgttttt atacaccaaa aaatataact tagccaggaa caaacgcctt tgaaaaacta
    18481 tgcattacct tgtaattgaa ggaaatatcg gtgcaggcaa aacaaccctt gcaaccatgc
    18541 tggcaaaaga acttaatgcc aaactaatcc tggaacaatt tgccgataac ccctttcttc
    18601 ccaaattcta taaaaaccct gaacggtata gtttcccgct tgaactctca ttcctggcag
    18661 agcgatactc acagctcaat aacgagttac gttccgcttc ccttttccac tcattaacaa
    18721 ttgcggatta ctacttcatg aaatcgctaa tttttgctca gaacacacta agcggcgatg
    18781 aacttcagct ttacaagcaa ctattttcca ttatttacag cactttgcca aaacccgacc
    18841 tatatgtata cctacattta cctacaaacc agttgcttaa aaacattcga aaaagaggac
    18901 gcgattacga acaaaccata accgctgaat acctgaacgg aataaccgaa ggatatttcg
    18961 attttttcaa acaacatcct gaatacactt ttttagtaat tgatacttca aaaattgact
    19021 ttgtggcaaa ccgcgatgat tttttaaaaa tcaaatcgac gatttttgac caacattaca
    19081 aaaaaggtgt taacttggtg tcgctgtaag acaaatgttt atttttgttc catgaatttg
    19141 ataaattgga atttaagtag tatgtttgta tttactaaat tgagtgctta caattaagca
    19201 gaattaataa ctaaccaact tttatatgaa aatgaaaggt ttaaaactat cgattattgc
    19261 cttggccggt ctgcttttgg ccagttgcgg cggggttgag aaaatgaaag aattacctgc
    19321 cggttttact ttaaaggtaa ccccaaaccc cgtagaagta catgctaaca aagtaaaggc
    19381 tacttttgaa ggtactattc ctccaaagta cttcaacaag aaggccactg ttgaaattac
    19441 ccccgtggtg gtttacaagg gtggtgagtt agctcttccc tcaaaggttt tacaaggtga
    19501 agacgttcag gccaacaacc aggttattaa ctacgaagct ggtggtagct ggaaacatga
    19561 tgtagagttt gattacaagg acgagatgtt cagctctgaa attgaactcc gttttgtgat
    19621 tacctacaag gacaagaaag ttcctttcga cgctcctcaa aaggttggca ttggtacaat
    19681 ggctacctac aaactggttg acattgatgt taagccagta atgctaaacg acaagtttgt
    19741 acgcgttacc cctgaggaaa aggaagctca aattaatttc gtaatccaaa aggctgacat
    19801 ccgcaagtcg gagttaacca aggatgaaat caagatgtta aacgattttc tacttgcagc
    19861 tgctcaggct aaaaacaagg agattaaaga gctcaagatt gctgcttatg cttcacccga
    19921 tggccctgaa aaactaaacg acaagctgtc gcaggatcgc ggaaagaaca ccgacaagta
    19981 ccttgctgat gtgttcaaga aagccaaagt tcaaggcgtt acccctgcag ttgaattcac
    20041 tgctgaagac tgggatggct tccagaaagc tgttcaggct tccgatatcg aagacaaaga
    20101 acttattatt cgcgttcttt ccatgtactc cgatccagta gtacgcgaga aggaaatcaa
    20161 gaacctttca aaggtttaca aggtattggc tgagaagatt ctcccacagc tccgtcgttc
    20221 aagaatggtt gccaaggttg atgttattgg ctactccgac gataccctaa agtacatggt
    20281 tgaaaacaac gcttacgaga atcttaatgt tgaggaaatg ctctacgctg ctacccttgt
    20341 aaacgatgtt aacaagaaga ttgaagttta catgaaagcc ggcgaaaagt tcaactgcgc
    20401 acgtggttac aacaacgctg cttactacta cattctgaac aaggatgttg ctaatgccaa
    20461 ggttgctctg gataagattc ctgccgatca gaagattgct gccgttacca acaaccgagg
    20521 ttgcgtagct atgcttgaag gcaagtttga tgaagctgaa aagctatttg ccgatgcagc
    20581 cgatgctggt agcgctgtta agtacaacct cggtattatt gcaattctta aaggacagta
    20641 tcctgcagct gctgaatacc ttgctggtag cgattcattc aaccagggtc ttgctcttct
    20701 attagcagga aaggttgatg ctgccaagaa cacctttaac aaggtagaaa cagctaaggg
    20761 ttactatggc cgtgcaattg ttggcgctcg ccttgctgaa gaagcaactg tactcgataa
    20821 cctacgcact gcttttggta aggacgcttc actaaaagat cgcgctaagg aagatgttga
    20881 attccgtaca ttcttccaga acgaagcttt taaagcatta ttctaagata aatacattac
    20941 taagaaacgg ggctgcacat gcagccccgt ttttttattg caacaaataa tacctgaagt
    21001 tcagcagaca ctaaactagt ggaaaagtta aatatcagtc gaccattact ttaacaaaaa
    21061 aatcccaaag cactatacca gccgagaccg aaatgttaaa ggagtgtttg gtgcctagct
    21121 gtggaatctc aatgcaaaca tctgaaatat ctaccacatc ctgtcctaca cctttaactt
    21181 cgtttccgaa taccaatgcg tacttctgcc cttttttcgg ataaaactgg ttaagcagca
    21241 ccgaatgctc cacctgttct actgaaataa tcacataccc cgattgtttc agtccttcaa
    21301 ctgctttgac tgtgctatca gcatactccc agtctactga attctcagca cccaaagcag
    21361 agcgatggat ttcagggtga ggtggaatac ccgtaattcc acataggtaa attttttcaa
    21421 tcaggaaagc atcggaggtt ctaaaaacag aacctacatt gtttaaactc ctgacattat
    21481 cgagcactac agcaattggt aatttttcgg aggatttaaa ttcatcaatc gattttcgcc
    21541 caagttcttc atttaacaac tttctgtttt taaccatatc cacaaaaaat ttaacaaatt
    21601 tagttaatcc tttttttgta acttagatgc attaaaaaaa tgatgttttt ctatgaatct
    21661 acatgagtat caatcgaaag aaatactaag gcaagcaggt gtacccgtgc cagtaggttt
    21721 agtagctttt agcaaggatg aagccattag ggctgcacag gaagtaatga aactcacggg
    21781 tactaacact atagccgtaa aggctcaaat tcatgctggc ggacgaggca aaggtggtgg
    21841 cgttaaaatt gcaaagtcca ttgatgaggt atcaacatac gcctcgaata ttttagggat
    21901 gaatctggta acccaccaaa ccgggcctca aggtaagctt gtacgaaagg tactaattga
    21961 gcagggtatt tatcagccgg gtccatcgga aataaaagag tactatgtaa gcatcctgct
    22021 aaatagagca tcaggaaggc atatgattat ttactccccc cagggaggaa tggacattga
    22081 agctgtagct gctcaaaccc ctgaactaat ctttaaggaa gaaattcacc ccgttgggct
    22141 tcaggcattt caggcacgta aaattgcgtt caaccttgga ttatcaggca aagcctacca
    22201 gcaaatggtt aagtttgttt tgtcgctcta cgaagcctat accaaaaacg atctttcgct
    22261 acttgaaatc aaccctgttg taaaaacatc gaacgacgat attttggccg cagatgccaa
    22321 gtgcagcatt gacgacaatg ctcttttccg ccaccccgat ttggctgagt accgcgatat
    22381 ttacgaggaa gaccccgcag aggtagaagc aggcgaacac aaccttaact ttgttaagct
    22441 cgacggtaat gttggctgca tggtaaacgg cgccggttta gccatggcta ccatggatat
    22501 tattaagctc tcaggcggcg accctgccaa cttccttgat gtaggaggtg gagcaaatgc
    22561 taaaaccgta gcagccggtt tcaggataat actaaaagac cctaacgtta aagccatact
    22621 tatcaatatt tttggcggca ttgtacgttg cgaccgcgta gcacaaggtg tggttgatgc
    22681 ctataaggaa atagggaata tccctgttcc tgtaatagtt cgtttacagg gcaccaatgc
    22741 acttgaaggc aaaaagctga ttgacgagtc aggcttaaag gtgtacagcg caatcacact
    22801 gcaggaggca gcaaacctgg taaagcaaat ggttaactaa aaaaaaaaac ggggcaaagc
    22861 cccgttttta tttacttctt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22981 caaatggtta actaaaaaaa aaaacggggc aaagccccgt ttttatttac ttcttggtgg
    23041 aacaggtact tatcccgaat agggagtaaa gtccacatgt tcctgttagt gccgttgcca
    23101 gaggtactat tgccagtaat ccccaccagc tttggtagta gattcctacc ccagttataa
    23161 ctaaagcaat cagaattctg attaccttat caattttgcc tacatttttt tccattgttt
    23221 catagtttaa tgattgcatt tctagaaaca aatcacagtg gaaaatgttt actaccactt
    23281 tattggtttg cgacggaaag gcatagtcat acatcgcgca cctcctccac cgcggggaag
    23341 ttcactacca tcgagagcaa caacatattt ttggtaggag ttcatatcaa cctttccagc
    23401 cacaatatct tttgctctga gaatttcaaa tccgtttttg ttcatctcct cgagcgtatt
    23461 gatatttcga ccatagccaa ttaccctacc ggggccaatg gcaacaaaat ttgctccgct
    23521 gtgccactgt tcacgctcct gtatccaacg atcctttgta ccaccacaaa ctattggttt
    23581 taaatcgata ccaaggctct tgagagcaac taggaggtta tcaaccgttt ttatctcctt
    23641 aaccttacct ttatcgattg tgatgtgaac ggtttggtaa cggttaggct gcagaattat
    23701 tggttcataa accatgcact tgtcaaaatc gagcatggtg aaaaccatat cgaggtgaat
    23761 gaacgattcg ggcttatagg gtaattcctg taccaggata tgccgtttcc tcttatcgtc
    23821 gcgagccaga aacctggaca gaatgaaatc gataccatgg gtattggttc ttattccatt
    23881 gccaatcaac agtatatcgt cgcgaactac aagaacatcg ccgccctcaa tggtaacatc
    23941 tgcggggttg ctaaaagtag tggggtttat ggtttgcgta ttaaaaatac cggaacgcga
    24001 gaatattgcc tccataataa tactctccct atccctaacg gcgttggcca ttttagctat
    24061 aagcacatcc tcaccaattg ctattgatgc atcgcgtgta aagtagaagt tatagagcgg
    24121 gcgaagtgca aacctctcct tacttaaaaa gttagtgagt gtattacgaa ccaatggaac
    24181 gccctcaatg agcatcctgg caagctgctt tgcatcaata tctaaaagat agtcgaatag
    24241 cgacatggca ttctcatgac gacatatctt ttcgagcaga tactccctat tctcctcgtt
    24301 cttaagtact ttttcaagca aatcaaccac ctcgtaggtt tttgccacct ttgaaagcac
    24361 gccactgagc tgcgagtact ccttacgggc aacgttcagg ttaagaatat cgctgtataa
    24421 tgctcgttga gcattttcgg gggtcatatt ttccacctcg gctcccggtg tatgcagtat
    24481 tacaccctca agctcaccaa tctcggaatt gagataaaca tttaccctct ccatagtaat
    24541 tgtaaatcaa atttattgca aagttaattg tttgcgcgga aaaactaaat gtgttaagaa
    24601 acatacaaat agctaacgtt ttgtattggt aaaaaatgac tggcctatta aggaaatttc
    24661 aaagtaattg ggtttaaaac aaaaagtgcc ccggagttat ccaaggcact tttctagcta
    24721 aacgattaaa tgtatttaca tgtaatcctc aattggtgcg caggtgcaca ccagattacg
    24781 gtcgccataa cctgcatcaa ttttgcttac atagggccag aatttattct gcattaccca
    24841 gggaagtggg taagcagcct gttggcggga ataagcatgc ttccactcat cggcggtaac
    24901 ctcaggggct gtatgcgggg cattggcaat aaggttgtcg gccttatcgg ctttaccgtt
    24961 cttgatatcc tcaagctctc ccttaatggc aatcatcatg tcaataaacc tatcgagttc
    25021 ctgttttgac tcgctctcgg taggctccac cataaggctt tcatgaaccg ggaacgatac
    25081 ggttggagca tgatatccgt aatccatcat acgacgacca acatcgctgg tatctacgcc
    25141 gtaggccatc ttaaagtggt gacaatcgag aatcatttcg tgggcaaccc taccggtttc
    25201 gccggtgtaa agaatcttat agtgatcctt tagcctggca gccaagtagt ttgcgttaag
    25261 aattgccatc tttgtcgcct gggttaaacc ctcggcaccc aacattagaa tgtatgcgta
    25321 agatattaca attatgctag cacttcccca gggagcagca gcaacagcag taatagcttt
    25381 ttcgccaccg gtcttaacca ctggatgtga agggaggaat ggagctaaat gcttagctac
    25441 tgcaattggg ccaacgccag ggccaccgcc accgtgtggg atagcaaagg ttttatgcag
    25501 gttaaggtgg catacgtcgg caccaatctc acctggactg gtaagcccaa cctgggcatt
    25561 catattggca ccatccatgt aaaccaagcc accattctgg tgaacaatat ccatcatctc
    25621 cctaattttg ctttcaaata ctccatgggt tgacggatag gttaccataa atgctgcaag
    25681 gttttgcttg tgctgctctg ctttagcgcg caaatcgtca acgttgatat tccccttctc
    25741 atcgcaagca acaaccacca cctgcatgcc tgccatagca gcacttgcgg ggttagtacc
    25801 atgtgccgac gaaggaatca gtacaacatt cctgtggccc tcgccacgat tgatgtggta
    25861 ctggcggatt accattagcc ctgtatactc accagctgca ccggagtttg gctgtaaggt
    25921 cacagcatgg aacccggtaa tctctgccag atattgttcc agttcacgga tgacctgcat
    25981 gtagccttcg gcctggtcag caggcacaaa cgggtgtaag ctattccact ccggccaact
    26041 tagcggcaac atggtggttg ctgggtttag cttcatggtg cagctaccca gcgaaatcat
    26101 gctatggttt agggctatat cgcgacgctc aagtttcttg atgtagcgca tcatttctgt
    26161 ttcggaatgg aaactgttga ataccttatc ggttaagaaa ggcgttttgc gacgaaggtt
    26221 ttcagggatt tcaatggttt ccttaatctc ggctatagaa ttgaacttct ttccagcaga
    26281 ttcggcaaaa actgaaagaa ggttgttgac atcggttacg gaagtggttt cgtcaaagct
    26341 aatacctacg gttccgtttg gcttgtaatt caggttgatt tcgcgagcta gagcgcgctt
    26401 gcggatatca tcgatggtta cacctgaagg caattctacc tcaagggtgt caaaaatatt
    26461 cttaacgttt tgcttgtaac ccataccaac aagttccttg gcaacctgaa cagcagtgct
    26521 gtgaacatac gaggcaatac ggcgtattcc ttcggggccg tggtaaacag catacatacc
    26581 tgccatggta gccaagaggg cttgtgcggt acagatattt gatgtggcac gttcacgctt
    26641 aatgtgttgc tcgcgggttt gcagtgccat acgtagagct ttgttgccat gcctgtcaat
    26701 ggaaacacct attattcggc ctggcatgct acgcttgaac tcatccttgc aggcaaagta
    26761 tgctgcatgt gggccaccaa atcccatggg tattccaaaa cgttgagtag aaccacaagc
    26821 aatatcggcc ccccactcgc ccggaggtgt aattagagcc aggcttaaaa tatcggaaac
    26881 ggctgttacc agtgcaccag cctcgtgtgc ttttgcagca aaagcggcat agtcgcgaat
    26941 gtttcccttt gccgatgggt actgaactat tacgccaaat tctcgtccag taaaattata
    27001 ctcgctataa caccctgtaa caacttcaat cccttgcgga tttgaacgag ttttaattac
    27061 atcgagggat tgtgggaaaa tatcctgatc aacaaaaatt acattcttgc ctgccttaac
    27121 agcctcgcgt gagcggaggt tgagcatcat gagcattgcc tcagcagcag cggttgcttc
    27181 atcaagtaat gaagcattag caatttgcat tccagtaagc tcaaccacca tggtttggaa
    27241 attgagcaat gcctcaagcc ttccctggct aatctcggcc tggtatggag tgtacgaagt
    27301 gtaccaaccg gggttttcaa atatgttacg caaaataacc gaaggggtaa cggttccata
    27361 gtaacccatc ccaataaaac tcttgtagag tttatttttg gatgcaacct tatgcatatg
    27421 ggtcaggaac tcatattccg acatgggagc aggtaggttt agcggttttt taagtctgat
    27481 attggccgga atggtttggt tgattagctc atcaaccgat gaaacgccaa ttacctttag
    27541 catttcaccc acttcatggt cgctaggacc attatgacgt gacacaaatt tatcggtata
    27601 catgttttag gttttttatt taaagattat cagcaaaatt atgaatttgg gttattgatt
    27661 ttaactaaac aaaagttttt tgcttatgtt ttttaacacc taaactacaa aaagggattc
    27721 gatgcccttt cgattccaat ggttgtgcta ttaccatggc cagggtaaac tatcacatcg
    27781 tcaggtaaca ccatgagctg ttccctaatg ctcttaatca gcgtttcgta attaccacca
    27841 gccaaatcgg tacggccaat ggataggtta aatagggtgt caccggttaa aataaatctt
    27901 tcctgctccg cataaaagca caaaccgcct ctggagtgtc cgggagtgtg aataaccttt
    27961 atactatcac cgttaagttc tatcatatct ccatggctga gtgtttcatc gattgctggt
    28021 actgtttcaa ggtataacca aaacatcatg gcatgtgctg gggccatttg aagcaaagga
    28081 aaatcatcga tatgggcaaa ggccttaacg ccaaattgct tctttagctt atcaattcct
    28141 agtatgtggt cgatatggca gtgagtgttt atcaaacatt ttaccgtaag gttttggcta
    28201 tgaacaaaat ccaagagttc ctgaaactca ctttcggttg agcagcctgc atcaaccaca
    28261 atggcctcgc cctgtggttg gtacagaaca taggtgttct cctggaaagg attgaaaaca
    28321 aactgtttaa ttgcaatcat tagcttacta ttttgcttta ccttttaaca acggaacaaa
    28381 aataaagttt ccgtgagccg attcttttat ggttccatca tctaatttct caatgagcat
    28441 catggtttgg ccataatcat tccctaccgg aattaccaag cgcccccctg gtgcgagctg
    28501 atcgacaaga gcctgaggga cttctgtagc gccagccgtt actattatac gatcgaatgg
    28561 actatatgga ggtaatccct ggtagccatc gccgtaatgg cagctaggcc gatagcccat
    28621 ctcctcaagt agagcctttg ccgcaagaaa caattcccgg tggcgttcaa tggtatagag
    28681 cctagcaccc atttccataa gaatggcagc ctgatacccc gagccagttc caacctccaa
    28741 aactttttgc atgggactaa cctgtagcaa ctgcgtttga aaggctacag taaatggctg
    28801 cgaaatggtt tgcccggaac caattgggaa tgcttggtcc ttgtaggcaa agcttaaaaa
    28861 accactatcc atgaaaaaat gacgaggcac cttaagcatt gcttcgagta ctcgcttatc
    28921 gcttatcccc ttggcagcaa cctcttccac tagcttgcga cgtaaacctt tgtgacgtat
    28981 actatcgtcc tggagcatat tttttaatat catttaaatg gcaagcaaat ttagtttttt
    29041 ataaagcata aaattccaac tgttactaaa aggttaataa attatgagga gtgagccact
    29101 gaaatgatta cagaacaatg tattattgca aaacattagc ctttaactat gacaacctgc
    29161 cttgtaaccg atagcaataa gttaattccg tatcaggggg agaatttttt caataacacc
    29221 attgaagcca acaaccaact gattgataag attttgcttg ggatatgtga aagcaactca
    29281 caaatagcaa ggctgtttaa ctaccctgaa ttgctggtag ttgacactaa cccaaaaacc
    29341 tcgtacacaa taattgccaa tgcactacga gccggcatta acgtattaac gccatcgctt
    29401 ccccatttca gcagcgaaaa aattcaggaa cttattacca tagcccaaga gattggggtt
    29461 gagtttggct ttttaccaaa atatagcctt gaacttccat caacccacag cccaatcata
    29521 attgatagcg taaaagagac aatcaaccta acctcaaccg aaaatttatt gcaacaaatt
    29581 gcaaatgact taatattcct tttatcacca ataaaatcgg aactccgcaa ggttagggtt
    29641 tactggttgc ccttatacag ccatccagtt tcaaccctga aactgatagt tgatttcaac
    29701 gacaactctt tgattaccta tcaaattaaa aacaagaaag aaacgagcat gttaaaggtt
    29761 gtgctaacaa ccgaggagta tgataaaact ttcaatatct ttgaagccca aataaaacca
    29821 gaatcaacaa aagaaacggt tatgcaaaac gtggattact tcataaacca acgaaagatg
    29881 ttgtacccgg ctgccatggc cctaaaatcg aagcaaatca tagaaataat cataaaaaag
    29941 atctacagct agcaatattt taaatgtttc gtagttatat tgaataaaag ttgcgacttt
    30001 gaacaaaaaa aagcaggcat tccggtattt gcttttcgat gtagctggta gcgccctggc
    30061 atggacgtta ttcaatctat accgtaaaag ggtaattgaa tcaaaccttt ttggcatcga
    30121 cattcgtttc aaccctgact ataagtttta cctagggctt gtattcttca cattattttg
    30181 ggtttcgatg tttggcttgg ttgggcttta ccacgaccca taccgaaagt cgaggctcag
    30241 agaatttgga gcctcgctaa ctattaccat tattggaacc attgtgcttt tctttgttct
    30301 gctgattgac gattacgtgg tagcttacca gaactactac aagattttgt tggccatgtt
    30361 tctgcttgta tttgcgctca cctatacacc ccgacttgta attacaaccc aaaccattca
    30421 ccggatccat aacagaaaaa ttggctaccc aaccataata gtaggcagtg ggaaaaaagc
    30481 catgcaaacc tataacgata ttaactcgga aaaaatatcg caaggataca gaataattgg
    30541 ttttatcgat ttacccaatg gcgtcagggt tgaccaatcg cttattttgg gaagtgtaaa
    30601 caacataccg gaagttgtag caagatacgg aattgaagag gttattcttg ccattgacaa
    30661 tgaaagccca caaacgctaa caaccgtttt aaacctactt gttccgctaa atgtaaacat
    30721 caaagcaata cctagcatgt acgatatttt gctggggaaa gtaaagctga atcgtattat
    30781 tgggactacc ctggtagatg tctcgtttga gcccatgcct gcctggcagg caaacctaaa
    30841 ggaaatgctc gactacttta tagccattgt tggtcttttg ctctcagccc cagttgcgct
    30901 tatactcatg gctgtaatta aaacaacatc aaaaggccct gttattttca aacaggaaag
    30961 gattggacag catggtaaac cttttcatat atacaagttc aggagtatgt atgtggatgc
    31021 cgaggcaaac ggacccgctc tatcaaacca aaccgaccca agagtaactc ctgtaggtcg
    31081 atttatgcgc aaaacccggc tcgatgagat tccaaatttc atcaacgtac ttaaaggcga
    31141 gatgtcaatt gttgggccac gccccgaaag gcaattctat atagaccaac tagtaaaagt
    31201 tgccccccat taccttcatc tacaaaaggt aaagcctggt attacatcgt ggggacaagt
    31261 aaagtatggc tatgccgaaa atatcgaaca aatggtagag cggctcaagt acgacctttt
    31321 atacctagag aatatgtcgc tttacatcga ttttaagata atgatataca ccatactaac
    31381 catttttaag gggaagggag tttagcatcc ctttagctca aatttctgga tagccctata
    31441 ctctggccct tgcggtttta aaatgctttc gtagagcacc agttccttaa cctcttgtac
    31501 ctggaatgat ttatttcggt attgctgaat tgctgcattc aacttatcgg aattgtgcat
    31561 aaattttact ctgcccaggg taagatgcgg attgaatttt tccttatcat cgctaaagtc
    31621 aaactcgctc aaaactttat caactgaaag tttaagttca ttaagcaact tggggaaatc
    31681 aactcccacc caaataaccc ttggcgatgg gcgcccaaaa taacctatac ctttaaggat
    31741 tatgctaaac ggttcaaatt tttttatagt tgcttttaac atttcacata tacttgggat
    31801 ttgagcggaa ggggtatcgc ctaaaaagaa aatggtaaga tgcattattg ccgggtcaac
    31861 ccatttgatg ctatcgagtt tatgattgcg cttcaactct tgaagcaggt taatgatatt
    31921 atcctgagga ttaacatcaa ttgccaaaaa agtacgttta gtttccatga tgttcccatt
    31981 taggtattgc gtataaataa aaaacaaact atcttgttta gcaaatttaa aacgtttgca
    32041 ctaatgggaa acaataaaaa atttgctgta atactttcag gtagtggcgt ttttgatggc
    32101 tctgaaatcc atgaggcagt catgaccctt tacgccatag caaaacatgg tgcaacttac
    32161 gaaatttttg caccaaacat aaaccagtat cacgtgataa accatataga cggaagcgtg
    32221 atgaacgaaa aaagaaacgt gctggttgaa tcggcccgga ttgcccgcgg caagataaaa
    32281 ccgttaaatg agtttaaccc tgaacttttt aatgccctca tcattcctgg tggttttggg
    32341 gttgccaaaa acctttccac ctgggccttt gatgggccta aatgcagcat acaccctcag
    32401 gtggaagcag ccataaaagg gatgcataag cttaaaaaac ccattggagc gctgtgcata
    32461 gcacccgtgc tacttgcaaa aattattggg aatgccaccc taaccattgg gaaagataca
    32521 tcaacagctc aagcaattga atcgttgggc gccaagcacc aaccaaccgg ccacgcacag
    32581 gttgttgtgg atgagaaaaa catgctgttt actacacctt gctatatgct ggatgccaat
    32641 attgttgaca tatcgaatgg agccaaggct gttgtttcag aaatactcaa atggatttag
    32701 ccatgaaaaa acttgctctc ctggcaacca ttatagctac aatgtttagc tacagctgcc
    32761 ttaaccgatg cgatgttgat tgtgttaatc caccatcgcc actatggctt aaggttgttg
    32821 ataaaacaac gctccaaaat ttaatagctg aagggtttta ccacagggat accattaaaa
    32881 tctactattt tgatggtgta acaaaaaaat acgccaacat ttactatgtt ggaccagttg
    32941 gccaaaacga tataatacaa actaatattg gtattctaca accctttgag cagctcaaca
    33001 cgttttacct ataccttaac caatacgata ccgataccat actggtacga tacgtaaaga
    33061 gtagcgatgg ctgttgcaca gcctacccac tcgattcaat tgccattaac ggaaagtatg
    33121 ttgatgttga caatgttgac tactccttcc tgatcaaaaa gtaaactttc atataagccc
    33181 ctgctcgtaa aggaagatta gctcattgag aatcttatcc tcgccctgcg ggtcgaactt
    33241 ggttttaaga ttcgacccaa aaaggcgggc aatgccctgc cagaacgtgg cagaaaaacc
    33301 tccctttaac tcctttacaa gctcatatga agtacgccct gtttccctat cgatgagttt
    33361 aattaacatt ttcccctgcg atatggtaag ctttgtcaaa tccttctcaa actgatttcg
    33421 gagttccttt tcggccttgt caatgtattc ctttttctct cggtcagttt taagggtttt
    33481 caaattctca tttatttcta gtaacttata ctttgcaatt tgagcgtagg gatatgtttt
    33541 cttaaggttg tatatcattc tgtagtactg gctcatttct cttcggctct tgaaacggcg
    33601 tttacctacc ttaacaacat cttcaatctt gatatggggt acatactcgc catcgagaat
    33661 gattgtaggg tataagataa ggctatcagc ggatggggcc tgtgcgccaa catccttaac
    33721 tgcagcagca accagaaaag ctaccaagac agtacctgaa aatcttttca ttccaccccc
    33781 tcgattctta tgccaaaatt aataccacat caacccttat acaactatta tgcaaatttc
    33841 cttcaaattg tttcaaaatt gtactcaaaa atcaaaataa gtaatttatt taacacactg
    33901 tatgtgaata tattaacaca ttcaaggcaa tattttgatg tacttttgtt gtagcttatc
    33961 gaaaacgttt tcgaatgatg agaaagtgtt gaaattgggt tgtgcaatcc atgaagcccc
    34021 aaaaaagttg aaatggcttt taaaagaatt tagcttgcac accgaaaacc aatttctaaa
    34081 cctaattgag tatgaaaaaa atcttcggag ttattagttt aatcctttgg tgcggtagcc
    34141 tgctcgcaca ggaaattaac gtgagcggca ctgtcacatc gagcgatggc gcaactctac
    34201 caggggttac catcgtagta aaggaaaacc ctacaaaggg tgctataaca gacgcaaatg
    34261 gtaagtacac cataagtgta ctgccaaacc aaacgttaga gttctcgttt gtgggaatgc
    34321 aaacccaaac tgttcccgta ggcggtagaa ctcaaattga tgttgtactt caggaatcaa
    34381 cccaaatgat tgaggagctc attgtggttg gttacggtgt acaaaagaaa agcttagtgt
    34441 cgtcgtcggt atcaaaagta tcgagcgagg atattgagaa ggctgcacct acacgtgttg
    34501 agcaagttct tcagggtaga actgctggtg tacaggtaat agccaactca gggcaaccgg
    34561 gtgaagggtt cacggttcgc atccgtggtg taggcaccaa cggcacatcc gaccccttat
    34621 atattgtaga tggtatgcca gttggtggaa ttgactactt aaacccaaac gatatccaat
    34681 cggttgaaat acttaaggat gcgtcagcat cggctattta tggtgcacgt ggtgcaaacg
    34741 gtgttgttct tatcacaacc aaacagggtc agaaaggcaa gttctccgtt aactacgatt
    34801 actccatggg ctaccagaac gcatggcgta aggtgtcgct acttaatgcc aaggagtatg
    34861 caattatcat gaatgagagc tacgcaaacg acaaccgccc cattcctttc cccgatgtta
    34921 atgcagttgt tcaggctgta ggtaatggca ccgattggca ggatgaactt ttttacaaga
    34981 atgctccaac atacaatcac cagttctcgg ttagcggtgg aaacgaaaat tcaaccttcc
    35041 tttcatcgct ctcctacact taccaggatg gtatagttgc caagggtaaa tcgaactacg
    35101 aacgctacac ctatcgtcta aactcaaccc ataagattgg caagttcagc atgggcaaca
    35161 acctggcata taccaataag gtaactcgtg gtattgaccc caacgccgaa tttggtggcc
    35221 cgctagctaa ggctgtaaat atggatccca taaccccagt aaaaaacccc gatggaacat
    35281 ggggcatttc aaactatgcc acacaggaag ttgttaaccc cgttgcttac cttagcgttc
    35341 ttaactcaaa ctactctgaa aacaaactgg taggaaacgt ttggggtgaa ctggaaatta
    35401 ttaagggatt aaaggcaaag accaatttta gcattgatta cgctaacggt tcaagccgta
    35461 catttgttcc ggtatacttc cttagcgata ttgtaaaagc cactacatcg gaagcctccg
    35521 gtactgttaa taaatggttc acctggcaga gtgagaacac tcttacctac aacaacacca
    35581 ttggccaaca caactttgct gccatggtag gtatgacagc aaataaatac taccatgagt
    35641 atattggagg taagaaaaac gacctaattt ttaatgattt tgagcatgct tggattaata
    35701 acggtaccga cgaagacagc taccgctcat ggggtggtgc cgatgagcac gccctgcttt
    35761 catactttgg ccgtgtaaac tacgactttg acggtaaata tgtattcgag ggagtattaa
    35821 gggccgacgg ttcctcaaac tttggttcaa ataaccgatt tggatacttc cctgcattct
    35881 cagctggctg ggttatgtcc aaggaagaat ttctgcaagg ttttacacct ttatcgttcc
    35941 taaagctacg ctttggctgg ggccgtaatg gtaacgaagc tattggtgct ttcaagtaca
    36001 cttcgctaat aggttcaggc agcaagtaca cttttggtac cgatgaggta atcacccttg
    36061 gttcaaatcc tgtaggtata tcgaatcctg acctaaaatg ggagacctca gagcaaacca
    36121 acgtgggtgt tgatgcccgt ttcctttccg ataagctaac ttttactctc gacctttaca
    36181 acaagaaaac taaagacctg ttggttgtag ccccaatacc cggatatatt ggtaacgctg
    36241 cacccacagt aaatggtggt tcagtaagta acaagggtat agagtttgaa ctgggttaca
    36301 aaaccctaat caatgattta ggaattaatg ctaacctatc gggtgcttac aataaaaacg
    36361 aagtgctttc cattaacaac agcgagggta aaatttatgg tgcaggactt gctgtaggta
    36421 tgtataatat ttgcgtaatg gaaactggat acccaattgc ctatttctgg ggttataaaa
    36481 ctgcgggagt attccaaaac gagcaacaaa ttcaaaacta taagtcatcg gatggtacag
    36541 taattcagcc caacgctaaa cctggcgacc taatttgggt tgataaaaac gatgatggta
    36601 ctattgacga taaagaccgt accatgatag gtaaccccta ccctgacttc acgcttggta
    36661 tcaatttagg gttaacctac aaggatttcg attttaatat gttctggtat ggtgcatttg
    36721 gacaggagat tttcaaagga acacgccgtt acgacctgcc catgtcgaac tgggatgcct
    36781 cagttcttga acgctggacc ggcgaaggca caagcaattc acacccacgc gtaaccatta
    36841 atgaccccaa ccaaaactac ttcagagttt ccgatttcta tattgaggac ggttcgttcc
    36901 tccgcctgaa gaacataacc ctgggctaca cacttcctca aaagttcagc caaaaggtaa
    36961 aaatctcaaa gttgcgtatt tatgccactg cggttaacct attaacattc accaagtatt
    37021 caggttttga tcccgaaatt ggcgcaaagt gggctcttga cgttggaatc gaccgaaata
    37081 tctacccaca gccaagaact ttcatgtttg gagtaaacct gagtttctaa cctaaaccgg
    37141 tacaatgagt atgagaaaaa ttaaaaatat atcaataatc cttttcatgg cacttggaat
    37201 tgttgcctgt acaaaggatt ttctggagtt acaacctcta actgacaggg ttgaggataa
    37261 cttctataaa accgagaaag acgcctttga agcagttgtt tcaatctacg atgttttgca
    37321 atggcaaaca ggtggtggct accacccatg ggatcttgta agtaatattc tttcggacga
    37381 tgcttttgca ggtggatcag gcccaggcga ccgtcctggc ttagttcgta tgggtaaact
    37441 ctcaaatttt gttactgacg aggaacctct aggcatttgg aaagacagat tttcaggcat
    37501 ttacagagca aatctgttcc tttcaaaggt agacggtgtt gatttcaaaa ccgagggttt
    37561 aaaaacccgc cttactgctg aggctcgttt tctaaggggt tatttctatt ttgaacttgt
    37621 tcagttctac ggaaatgttc ctctaatcct taagcccctt gcaccatcgg agtatcaaca
    37681 atctgctgcc gatcctgctg atgtttacaa tcaaattgcc agcgatttgt attatgctta
    37741 ccaaaaccta cctgctacta ttactgctgc cgaaaaaggc agagcatcaa agtgggctgc
    37801 gggtgcattg ctggcaaggg tttacctgtt ccataaaggt tatggtaaag gtgttctaaa
    37861 tatcacaacc gacctaaaag ccgatgataa aacctttaca gaaacagaca ttgaaaccat
    37921 tattgatgat attgttgaga atagcggaca tggactagta gcaaactatg ctaacctttg
    37981 gggtgtagat aacgagaata acaatgaatc gatttttgag attcagcaca cccaaaaggc
    38041 tgactggggc gactggggat ggcgtaacgg taccgaaggc aactgggctg ttgtaatgac
    38101 tggtttccgc ggtgttgaag atcccattta cgagtccggt tggagttttc agcctgccag
    38161 ccaatcactt gctgatgagt tcgaaccagg tgattcgcgt tactcagtat caatacttga
    38221 tgctgcagcc gaaagtttgg tttacaagcc tgaaaactgc taccagcata ccgggtatgc
    38281 attcaaaaag ttctaccctc gtaaggctga caggcccgcc actaacgatg ccttgaactg
    38341 gccttataac cgtcccgtaa tccgctttgc cgatgtactg cttatgggtg ctgagctata
    38401 cctcgacaac aatcttggta aagcacagga ctactatagc cgtgttcgta aacgggcttt
    38461 tggcgatgca cacgttgcac ctactctaac aaacgatcag gctggtcttg acctcattta
    38521 ccatgaacgc agggttgaat ttgctggcga agggctccgt tactgggatt tgctccgtag
    38581 gggtttaacc tatgctgaaa caaagattaa cgcagcagca ggtgcaagtc ctctggatga
    38641 aacattcagc tcggcaacta agggcttgtt gccaataccc cagacagaaa ttacactttc
    38701 ggggaataaa ctaaaacaaa atgctggtta ctaataaatt aagaagagct atgaataaac
    38761 gacacatcat attttccgct ctggttcttg ttgggcttac ctggtttggt tgcgagccca
    38821 tggaactaga taaaccaaat attgggaatg cccctacagc tgagcagcta gattttacca
    38881 ttacccctgg cgatgatgca tttcactttg taattactaa cacatctaca gtaaaaggta
    38941 ttgcaaactg ggatttgggt aacggtatta aagccgttgg gaattcagtt actgcatact
    39001 accctcttgc taaaacctac aatatcactc taaccctata tgcctcgggt ggtagtacct
    39061 caaaaacaaa agagtttgaa caaactgaaa cggattggtc ttaccttgaa agtcctatca
    39121 ttactgccct aacaggtgga gttgatgcac ctaatgggaa aacatgggta atcgatagcc
    39181 tttccgaagc acatcttggt gttggccctg ccgattcata ttcaccagta tggtgggctg
    39241 ctgctcccct tgctaaatcg ggtcactacc tctacgacga tgaatttacc tttaagcttg
    39301 tgggatttac ctataccatt gacactcatg gcaaaaccca tgtaaaccac gatggtaatg
    39361 ccgatgagga tggcttatcc gctggttatt atgtatcatc gttctggaac gacgcctacg
    39421 acatcgacat gaataccaac gatgccgccc gtggtgaact tacatggtca atcagcgagg
    39481 agaacggtaa atactatata cagatctcga gtcagtcagg caatatctct tacgacgatg
    39541 gcaacccccg caaatacgaa gttcttgaat ggaacgaaaa cttcttccac ttacgttctg
    39601 ttggtggtgc tgcccgttac cataagataa ttcctaaagg gtatgtaaga cctactatat
    39661 catatacact aaatgttaca gcaaccgcta accccaacga gtacacattt gctcttgagg
    39721 atttaacagt accctcatca tttaccattt cgaatattac ttacgacttt ggcgatggct
    39781 ccagctacca aaccaccaat ggagccgagg tggtaactca cacctacatg cgtaagggaa
    39841 attacaacac caaggttaca gtaacagcta acgggcagga ttttgttaaa acctcaacgg
    39901 ttgccgttgc cgctaatcac ccaacctacc aggagtacct gctcgatgct atggtaatgt
    39961 acaacgactt tggcgaaacc acccttgttc ccatgcaggt tgatcagtcc gatgggggag
    40021 ccacacttga aatagtaacc aaccccaaca ataccctcta cccgaaccgt agtgcacatt
    40081 gcgctctatt cacaaaatac aattcccagt gggcaaacgc atttactcgc ctacccgatg
    40141 gctaccgctt tgaccttacc aaacaaaccg tatttaaggt tttggtttat ggtaaggctg
    40201 gcgaccaggt acttttgaaa ctcgaaaata ctgaccgagg cggtaatgct tggcaaactg
    40261 gaacccatga tttgatctac accatccaga atgataataa atgggaaatt gccgagttta
    40321 actttgccgg agttggtgca ggttgggatt ggacaggtga cattttcacc ggcgatgtaa
    40381 ccactgactc aaggtttaac aaggacttct acaacgtggt tcgcataatg atcaaccccg
    40441 gtaacaacgg aggaaccttt agcgttcacc tcgacgattt agctgggcct cacatcgaag
    40501 ggcttaagta aactattctg ttcacagggt aacccttaac gggttaccct gtttaaaccc
    40561 gaaaaacgga aaatgatgag attgaaatta ataattgcag ttgtaacgtt ggcagcatgg
    40621 tcgtgtagtg gcaataaagc cacaatggac cattcggggg gcaaactatc tatcgatcca
    40681 gcaattgaac ggaaagttga ctcggttcta aagcttatga ccattgacga gaagataggc
    40741 cagctaaacc agctaaccgg taatggagag accactggcc ctatcacctt tgccactgaa
    40801 taccaggatg ctattcggaa aggtgaagtg ggaagcatgc tcaacgttaa tggcgcagcc
    40861 tacacctata aggtgcaaaa aattgctgta gaggaaagcc gtttgggtat cccgctcatt
    40921 tttggctacg atgtaataca tggctataaa acaatattcc ccattccgct gggcgaggca
    40981 gcctgttggg atttagatgc catcgaaaaa tcggcacgta ttgcagccat agaggctgct
    41041 gctgcagggc agcactggac ttttgcacca atggtagata ttgcccgtga cccacgctgg
    41101 gggcgtgtaa tggaaggagc cggagaggat cctttctacg gttcgcttgt ggcgaaagca
    41161 cgggtaaaag gttttcaggg ctcatcgttg gctgacccat caactattgt tgcttgcgcc
    41221 aaacattttg tagcctatgg agcagcaatg ggaggccgcg attacaatac ggtagatatt
    41281 tcaaacagaa cactgcacga agtttaccta cccccattca aggctgccat tgatgctggt
    41341 gttggaacat ttatgtcgtc gttcaacgag ctaaacgggg tacccgttac tggtaacaag
    41401 gaaatggtta aggatatcct aaaggacgaa tggcagttta atggatttat tgtttcggat
    41461 tggggttcaa tacgcgagat gctggttcat gggattgccg ctgacgacta ccaagcagcc
    41521 gacctagcca tgaatgccgg aatagacatg gatatggagg gtcatatcta catacatcag
    41581 cttaagaagt tacttgccga tggcaaaatt accgaaaagc agattgatga tgccgtaagg
    41641 cgtattcttc gcattaaatt catgcttggc ctgtttgatg acccttaccg ctactgcgat
    41701 acaatccggg aaaaagagtt aatacttaac cctcgccacc tggagattgc acgtgaggtt
    41761 gcacgtaaat cgatggttct gctaaaaaac gatggggtgt tgccacttag caaaaaactt
    41821 aacaccgttg cagttattgg cccattagcc gataataccg aggatatcat tggaacctgg
    41881 tcagcaaggg gtgaaggcaa ggatgctgta tcggttttga ccggtattcg tcaaaaatta
    41941 cccaacacaa aggtaattca cgcaaagggt tgcgatatta caggcgattc aaaagctgga
    42001 cttaccgaag caatagccac agctcgcaaa gccgatgctg ttgttttggt agtaggcgag
    42061 gcggccatga tgtcgggcga ggcgctcagc agggctttcc tcgatttacc tggagttcaa
    42121 aaggatctgg ttttggcaat caatgaactt aaaaaaccca tggtggttgt tctcatgaat
    42181 ggtcgtccgt taaccattga gtggatggat aatgcagttc ctgctattct ggaagcatgg
    42241 ttcccgggca caatgggagg tcctgccgtt gccgatgtac tttttggcga ctacaaccca
    42301 tcgggcaaac tgccaataac cttcccccgt aatgttggac aaattccatt attctattac
    42361 cataaaaaca ccggcagacc aagggtagaa accgaaaggt acacctcaaa gtatattgac
    42421 agtccaaata caccgcttta cccatttggc tacggactta gctacacctc tttcgattac
    42481 agtaattttg gtgttagcac caatgaaatt ggcatgaccg atacccttaa agtttgggta
    42541 gatgtgacca ataccggaaa ttacgatggt gaagaggttg tacagctcta cattcgcgac
    42601 atggtgggat ctgttacccg accagttaag gagctaaagg gttttgagaa ggtgttcatc
    42661 aaaaaaggag aaaccgtaaa ggtaatgttt aaacttaccg caagcgacct ggctttttat
    42721 acacgcacca tggaatttaa agctgaaccc ggcgatttca aggtgtttgt aggtggcaac
    42781 agccgcgact tgcaagaatt gagctttagg ttgaagtaac tttgggtaaa aggctttcgg
    42841 attaattgtc caaaaaaagt tttctttcgt ataagtatgg ttaggtgaga tggacaattc
    42901 ccgaaagcct ttttcttaaa aacaaaaaaa aagaatgagc agaaagttta tcaatagcat
    42961 catagcacta ctactagtag ttccctttac aagccttgct caggttaagg tcctggtatg
    43021 gaacgatgag tttaactaca caggattgcc tgccactgat aaatggggtt acgatgtagg
    43081 tggaggaggt tggggaaaca atgagctaca gtactacact aatgcacgaa ctgaaaatgc
    43141 tcgcgttgag aatgggaacc tagttatcga ggccagaaag gaacagtacg agggcagcga
    43201 gtacacctcg gcccgattag ttacccgcta taaaggcgat tggctttacg gtagggttga
    43261 aacttacgcc aaactgcctt cgggtactgg gacatggcct gccatttgga tgctacccac
    43321 caactgggtt tatggcagtt ggcctaagag tggtgaaatt gatatcatgg aatatgttgg
    43381 ctacgacccc ggggtagtgc acggaagcat tcacaccgaa gcctacaacc atgttcttgg
    43441 cactcaaaaa accgcaacaa tctcggtacc cgatgccgaa accgctttcc atctttacgc
    43501 tcttgaatgg acccccgaaa aaatcgacat ctatgttgat aataataagt acttcacctt
    43561 cgataatgag cacaaggatt acaaaacctg gccattcgat caggttttcc atcttattct
    43621 aaacattgcc gtaggcggca actggggtgg cgctcaagga gttgacccca acatttggcc
    43681 acaaaaaatg tatgttgatt atgttcgggt ttaccaatac atcgatgtat cggccttgaa
    43741 ggtttcgggt cccgaatacg tttctccaaa ccaaacgggc gttacattca gcattcagaa
    43801 tgttgccgat gcaacgttca gctggagtgt ccctgcaggt gcaaccattg ttagcggtca
    43861 gggaactaac tcaattgttg taaactgggg agatgtgcag ggtgatgtat ctgtagaaat
    43921 tacacacccg caagctggtg gaacatacac caagacagta aaaacagcgg tcattccaac
    43981 cggcgatagg tttagcctgt taaacgtaaa agaaaatgga cttattggtt gggctggttt
    44041 aaataccagc cccaacacca tcacgctttc agctgtcgat acgcttttga aggttaagta
    44101 caatatcact aaacctgaag actttccgta tttcacctat acatttcaaa accctgtgga
    44161 catgagcaac cttacagtcc tcaatgtgga gatgatgacc tacaaccaaa gcaaatcggt
    44221 agtaatgcgc gccgaccttt tcgacaccga tggccggcta accgatgtat cgcctgtttt
    44281 ccgcttttac cccgttgaac cccatggtgt attccactac tactcattcg actttaacaa
    44341 caactggggg tcgaacactc ctgaatatgg aaagcaagta aactaccatc aaattgcagg
    44401 tgttcgattt tatgtaaatt atggcgttta cggcaaagcc gcatctgatt cattgtggat
    44461 ttcagatgtt gttttgagca atacactgct aaccatcttc tcaccaagtg agaataaaat
    44521 aagcagattt gacatttacc caaatcctgc atcaacctat attaaaatca actcaagcgc
    44581 tttaaaggga aatgtaaaaa ttcaagtaat taacattgca ggtgttcttg taaaagaaat
    44641 cattgaaaat agtgatggtc aaattaatat ctcagacctt ccaaatgggt tttacagcgt
    44701 aagaataatt acacatacaa acttgctaca aactggcagt tttattaagc tttagcctta
    44761 caggttcagc ataaaatcaa ccaggtttgt atcgctttca attttgagct ttttccgtag
    44821 cctgtatcgg cttatttcca ccccgcgaac gctaatatta agtatctggg caatatcctt
    44881 tgtggtcatg ttaaggcgaa ggtaagcaca caacctaagt tcgctgggtg ttagctccgg
    44941 atacatctcc ttaagcttct taataaaccc gctatgaacc tcatcgaaat ggtgctcaaa
    45001 gcgctgccat tccttgtcca tcttcaagtc gttgtcaata tttcggatga ggtcgagcac
    45061 ttctttctga gaaacagggt taattgattt agcaatcact tcaagcctca accttagcct
    45121 gttaaggaag tcgttcttgt gcgcaatttg cattgcagtt gcggctagtt cacgattttt
    45181 gtgttcaatc tgtacctgaa gattctctgt tttaagcctt atgatctccc gttccgcctc
    45241 aagcacttga cgcttatgct cctcctcctt tacttttagc tccaaattct tttgctcttt
    45301 tacccttgca gtggcacgtt gaaccttcct ccgaacaaga atgcgaagcg tataaaaggc
    45361 agctaccaaa agtaccaagt aaagaataaa agcataccag gtaaagtacc agggcagacc
    45421 aacctgaata tgcacaacag tgggttcaga tattctgccc attgcatcgc gtacacgaac
    45481 cattaggttg tactcaccat agggaatttg gctaaaatcc ttagcgttat cacgctgcca
    45541 cggactccaa tcgttatcgt agcccttaag caaaaagcta aatgtaattt cgcctgcatt
    45601 gtaaacaggg gcagccgctg taacccgaat tgaattttgg ttgaatggaa tatacaaccc
    45661 tccattgcct gttacagcat aatcgttgaa aatcacactt tcatgcttac cagttgagct
    45721 gatacttttt acagcacaat acaactcgtt tttttgcagt tcaggattat aaaggctgtt
    45781 gtaatacacc aacccatttt cagtaccaat gaatatatta ctatgattgt aaacatagat
    45841 attttcaaac gaagctacga ggttcgcgcc aaatcggcta acgggggttt gataaaccct
    45901 tttagctcca tctgcttcgc ggactaactt tagaagacca tttgacttta atatccatat
    45961 atttcctttt tcatcctcaa ccatacgccg aacgcctgta gcatcaccaa taatctgatt
    46021 cagctcctgg tatggctcca tattatcggt aagggagtta tacctataca caccttgtga
    46081 ggtaaggaat acaacctgat ttccaatctt ttcaacattt atggcaaact tacccggaaa
    46141 cccccttgta ttatcgtaaa gggttgaaaa cctaatgcta tccttgcttt ggttaagcat
    46201 caacttaaaa atacctttat agccgtgggc catccaaata ctctcgttat catcctcaac
    46261 caacaacctt gacgactcgt tgaatcctgc aatcttttta aggtacctcc attcaccatt
    46321 agcttttgag tacagatgaa ggcccttata cttaccaaca atcataaggt caggaacgct
    46381 acgaatgggt aaaaacatcc acgagccctc ctcgggtgaa atcagcctaa cattcaaccc
    46441 cttatcaata ataaaagtac caaagttatg gccacagtag agcttgccat cgctctcaaa
    46501 aagcgaccat acctgtcctt tggttaaagg aaccatcctg aaaggcttgg tgccatctac
    46561 aaaaacgcca ataggccatg aggccacgta aagcccctgg ttggtaccta caaatatttt
    46621 cccatcaaaa atttttgtgg catacacagc cccttcaagc cctaacccct caccaataaa
    46681 gctgaggggt gagttaagct caatgtagtc gatcccgtta tctaaaccca accaaagatt
    46741 attggcatta tcaacaaaag tgcttaaaat ggtattattc tgtaacccgg ttgatttatt
    46801 aataaactta accagatttc cctgatcgtc ggtaatgatt aacccgttct gaatggtacc
    46861 aaaggctatg taaccaccac ttaccctcga tgccgaaaac acctggtacg ctttaagcaa
    46921 atcgttaacc tgccctttcc agggtgtaaa ggtaattcca tcgaaaatcc aaacgccgtg
    46981 gtttatggta gcaagcaagg ttttaccgcc cgatatttcg aagcatgccc aaatttgctt
    47041 tccgttgaaa ataccgttat cgttaaatct atcaaagcta ccattctggt aacggtaaat
    47101 atttccctcc ttaccctgca cgtagtagtt gccccgcatc acaaacgaga actgaagcag
    47161 ttcaggggct ctaaagacgc gcatttcccc tttgctgtat tctagaatgt ggctaaaagt
    47221 ttgaaaaatg tattttggac cgatcttaaa tatccgccaa gcctcatcaa aatttacatc
    47281 ggttttaggg actaggctga ttaatgagtg ataaaccata tcggcctgac ggttatatac
    47341 aaagtagcct atctcctcaa aggcccccac aaaaattgta tcgccgctac agtaaacaga
    47401 tcggactagc tggttattag gcaatgggaa taaatcccag ctattgccat cgtaacggag
    47461 caacccgttg ttgttggcaa aatataaaaa tccacgacta tcctggcaaa tagaccagtt
    47521 ttgggttcct gcgccatata ccgatttggg gtaatgcttt atgatgggta accctgcagt
    47581 attgatctga gaaaaagcat ttgttgcaaa aatcgcaaaa gcgatgaata tcagctttct
    47641 catctatcct tcattaatga attagcacag aaagttatga aattaaatct aaatattaaa
    47701 aatcgcaaac aagtatcaat atataaacat ttgtttatta gttacttgaa ttcagaaatc
    47761 gatttcggaa ttacaacact tgttaacctt tcaccacggg tcaatatcgc caaaaaagag
    47821 taaatttgtg ctccactcag taaacaaact tgaacttgta aacgtttttt gaataaggaa
    47881 ttttcagaaa gtttttaacc ttttaacagc acactatagt atgattaaaa gtaaagtcct
    47941 tttaatgatt ctcgatggat ggggaattgg gaacaaagac aaatcggatg taatttatag
    48001 cactccaaca cctaacatcg ataggctcac cagtaaatac cctaacgctc agctactaac
    48061 cagcggggag aatgttggat tgcccgatgg tcaaatggga aattccgagg tgggacacct
    48121 caacataggc gccggacgaa ttgtttacca ggatttggtt cgcatcaata aagatatcaa
    48181 ggagggtaaa ttggcccaaa tgccagttct ggttaatgcg ctacaatatg ccaagcagaa
    48241 taacaaggct gttcacttta taggattggt ttccgatggt ggcgttcact cgctggacaa
    48301 gcatctgtat aagctatgcg acattaccaa ggaatacgga ttaagtaagg tttacataca
    48361 tgcccttacc gatggtcgcg ataccgatcc acaaagcggt cttggctttg tgaaaagcct
    48421 tgaagatcac ctaaaatcaa gtaatggcgt tattgcatcg ttggttggta ggtactacac
    48481 catggaccgt gataagcgtt gggaacgcgt aaaggagggt tacgacctga tggttcatgg
    48541 taagggtaaa cccaccaccg atatagttag ggcaattgaa gagtcctacg ccgagggtgt
    48601 aacggatgag ttcatcaagc ccatagtgcg ggttgatgct gaaggtaagc ccgttggcct
    48661 tatccgtgaa ggcgatgtgg taatctgctt caatttccgc accgaccgac tccgtgaaat
    48721 aaccattgtg cttacccaaa aggatatgcc cgagtttggt atgcaaacca tgccactgca
    48781 ttacgttacc atgacccgct acgacgacac gttcaagaat gttaatgtta tttacgaaaa
    48841 ggataaccta ctgaacaccc ttggcgaggt ggttagcaat caaaacctga agcaactgcg
    48901 tattgccgaa accgaaaagt atgcccatgt aacctttttc ttttcgggtg gtagggagca
    48961 agagttccct ggcgaaagtc gcattcttat cccctcgccc aaggtagcca catacgacct
    49021 acaacccgag atgagcgcgc ccctggtaaa agatgccatt gttaccgaac tccacaagaa
    49081 aacacacgac tttatctgcc ttaactttgc caatggcgac atggtagggc acaccggtgt
    49141 ttacaccgcc attcaaaagg cagttcaaac agttgatgca tgtgttggtg aggtggttga
    49201 tgccgcaacc gctaacggtt acgaggtggt aatcattgca gaccatggca atgccgatta
    49261 tgccttaaac cccgatggct cacctaatac agcacactca cttaaccctg ttcccatcat
    49321 cctggtttcc gacaggttca aaaaggtaga taatggtatt ctggccgacg tagctccaac
    49381 tatccttacc atgatgggcc tgccaatacc ccaggaaatg accggaacgg ttctctgcca
    49441 gtaaaaagtt aggtaaatga tgatagaaat agacaacaag gtggtaagca gcgagatcct
    49501 ggaacaacgg ttttgctgca acctgccgcg ttgtttgggg aaatgctgtg ttcacggcga
    49561 ctcaggagca cccttaacac ccaacgaggc tcttaccctt gagcaaatcc tcgataaggt
    49621 taaaccctac atgacacccc agggtattgc taccgttcag gagcaaggag ttgcccttac
    49681 cgatagcgat ggcgatttgg ttaccccgct cattgacggc aaggaatgtg cttttactgt
    49741 atttgaaaat ggtatagcaa cctgtgccat tgaaaaagca tggaacaatg gtatagttga
    49801 tttccgtaag cctatatcgt gccaccttta cccaattagg gttaaagaat actcaacctt
    49861 tactgcaatc aactaccatc agtgggatgt ttgcgctccc gcacgtgagt tgggtgagca
    49921 aatagacctg cctgtttaca ggttccttaa ggatgctata atccgcgctt acggcaaaga
    49981 gttttatggc cagcttgagg acgctgcaaa attgttatct ttacgaacag aatagttaaa
    50041 tactaaaaca aatcttatgg atacaatcaa agcctacatc gaggagaaca aacaacgttt
    50101 tctcgacgag cttttcgggc tactccgaat tccatccatc agctcaatct cggaacacaa
    50161 acccgacatg gttaggggag cagaatactg gcgcgaaacg ctactcaaag ccggagccga
    50221 cagggctgag gtaatgccaa ctccgggcaa tccggtggtg tacggcgaaa aaattatcga
    50281 tccaaaactc ccaaccgttt tggtttatgc ccattacgat gtaatgccgg ttgacccaat
    50341 agagctttgg aaatcgcaac cctttgaacc cgaaattcgt gatggcaaaa tatatgcccg
    50401 tggtgccgat gacgataagg gacaaggctt tatgcatgct aaagcctttg agctgatggt
    50461 taaaaccaac accctgccct gtaacgttaa attcatgatt gagggtgagg aagagattgg
    50521 ttcaccaaac cttggtaagt ggtgccagga taacaaggag atgctaaaag ccgatgttat
    50581 tctggtttcc gatacatcaa tgatagggaa agacgtccca tcaattacaa ccgggcttag
    50641 gggattagcg tatgttgagg ttgaggttac cggaccaaac cgcgaccttc actcggggct
    50701 ctttggcgga gccgttgcca accccgcaaa catccttgcc aaaatgatag cctcgctcca
    50761 cgacgagaat aatcgcgtta ccattcccgg tttttacgac gatgtgttgg aggtaagccc
    50821 tgaagaacgc gccgatatgg ctcgtgctcc ttttaatctt gatgattaca agaaagctct
    50881 ggatattgat gatatccatg gcgaagccgg atatactacc atggagcgca caggaattcg
    50941 ccccacgctt gatgtaaatg gcatttgggg aggatatatt ggcgagggag ctaaaaccgt
    51001 tcttccctcc aaggcttacg ctaaaatatc gatgcgactg gtacccaacc agaaccacga
    51061 gaaaattgct caacttttca aaaagcattt tgagtcaatt gctccaaaat cagtaaaagt
    51121 aaaggttacc ccacaccatg gcggacaggg gtatgtagct cccacaaaca ctccagccta
    51181 tagggctgcc agtagggcta ttgaggatgt ttacggcatt aaaccggttc ctttccgcag
    51241 cgggggcagc attcccatta taagcacttt tgagcaagtg cttggaatca aatcaatcct
    51301 tcttggtttt gggcttgagt ccgatgccat ccactcacca aacgagaact atccacttga
    51361 acaatttttc aaaggaattg aagttatacc ccgattctac aaatatttta gcgaaaagta
    51421 aaaaattgaa aaaggggctg agcccctttt tttattttga tccattccgt ttcctttaaa
    51481 attattgtaa atttactgac agcttttact ctttttcagt aagttttttt ttaattcagt
    51541 aaaaattata acaactatac tacccatgga aaaattgaaa atgatatttg cactgcttat
    51601 gggattagca gtaattgttt cctgcaagca ggaaactaaa aagcaggctg ccactaccga
    51661 tgaaattgtt cagcaatcca taaaaagttg gatggaaaac cggaaagagg aataccccaa
    51721 ctacgtgcca ttagactttg gcaagctaac cccacgctac cggttcaaca gccgaaccca
    51781 tagcatagag attgctctaa atactgagaa atcaaaggat aacccaaacc agaatactct
    51841 cgatagccta aaaaggctac taaatgaaaa tagcaccgac tttttgggtt ataccataac
    51901 acaccaattc accacaaagg gaattgatgg tgaggtgaaa aaggatgaaa aacttttctt
    51961 tattgacccg caaaaccgtg taataacagt tctaaactcc gatgcatggg atttgataat
    52021 ggataaggaa ctattcttta ggcccgaaag tcccgatagt gtgaaataaa aaagccccca
    52081 tttaccgggg gctgtggtgc cacgtggaat cgaaccacgg acacaaggat tttcagtcct
    52141 ttgctctacc aactgagcta tggcaccagc gctcaattgc ggtgcaaata tatattcaat
    52201 tttttattcg tgcaaaacat caactaaaaa aaaatcaaaa acaccctaaa tggctatgaa
    52261 acagaatatt ttttccatcc taaaaagtgt aacactactt gttgttatca cctacagtaa
    52321 tcaaggggct ctttatgctc aaaatcagga ttgcaaagtg cttctccctg aaatatcggc
    52381 agcttacaca ggaaagtgta aaaatggttt ggctcacgga aaaggcaagg catggggtac
    52441 tgatagctac gagggaaagt tcaagaatgg actacctcat ggttttggta tatacacctg
    52501 ggcaaatggc gataagtacg agggtaactt ttataatggc caaatggacg gaaagggtat
    52561 ctttaagggc aaaatcaacg ggaaagactc agtatataca ggcttttggc aaaaaggagt
    52621 actacaccat aaaatcttac ctcctaagta ccaaattata aatgcaagga atgtgcagcg
    52681 ttataccata actaaaaccg gttcggaaaa ccgtattcta attgcattta cccagaacgg
    52741 aactacaaac aaccttgtga ccaaccttca aattgtttgc gatacaggct ccccatttaa
    52801 attaggcgag aaatccggct ttgaaaacat ccttttccca gtaaactgta aaataacata
    52861 tcaaacaccc aatgcactca gaaccgtttg gtacgatgta acctttgaat ttacaataaa
    52921 cgaagccggt gaatgggcaa taaatctatt taattaggcg atatcgccaa ctggctcaac
    52981 gcctgcccca ctcctcaggg ttttgtcgcc aacttttaag cagctcaagt tctgttgggg
    53041 taatgctacc ctgttctaat actgcctcaa ttaacactga gtagttcgat agtgttgcaa
    53101 actcaacacc agctttagca aagctactta ctgcctgatc gaactgatac gaaaaaatgg
    53161 ccagcaagcc aagaacctca caatgggcat cgcgaagagc atcaacagcc gcaaggctgc
    53221 tcattccggt tgatattaag tcttcaatta caactaccct ttggccgggt ttataatcgc
    53281 cttcgatttg atttgccaaa ccatgcgatt tgggcgctga acggacgtaa ataaaaggta
    53341 gatttagcct gtcggctaca agcattccat gcgcaatggc tccggtggca accccagcaa
    53401 taacctcaac ctgaggatac ctctccttta ttgccttaac aaagccatcg caaatggcat
    53461 tgcgaacctc tggaaacgat aatgtttttc ggttatcgca gtaaataggc gatttccatc
    53521 ccgatgccca agtaaacgga tttgcaggat taagctttat tgcgtttatt tgcagtaatt
    53581 tattggctat aactttgcta tccatgactt tgaattatac cattttcttt tacaatcgca
    53641 aatttattat cacttcggac ccccaagaag tgctcatagc cgactctaca tttttctgtt
    53701 ggggcgacac taagcccgaa gacatttcaa agttaatcga ttttttccaa agccataccg
    53761 aggtgtccac actttttgcc tgcacctccg accccaaaac aacaatgaaa catttcacta
    53821 acagctttaa ccacattgag gcagccggtg gagttgtatg caatgacaag aatgagattt
    53881 tactcattaa gcgtttgggc cgctgggatt tgcccaaagg caaggtagag ccaaacgaac
    53941 ctattcacga tgccgcttta cgcgaagtta gtgaggaaac cggggtaaaa ggcattactc
    54001 ttggaaagca aattacaaca acctaccaca cctatacttt aaatggcaag caaatgctaa
    54061 aatccacgca ttggtttaat atgtataccc ctaaatgcga taccctaaaa ccccaaaccg
    54121 aagagggaat agaacaggta aagtgggtta agcgttccga tatctttgta taccttgccg
    54181 atagctactc aagcctgctc tctgttctca aggagttgga ctaaatttcc cctttgcttc
    54241 ggagcaattg aaagaatcgg cgttcaaagc cctcctcggg cgaaggcgct ggtgaaagaa
    54301 tcattttccg gtcaggccca atcaaaaaat acgttggata tacacgtata tcgtaatcct
    54361 tgagtatttc cggtttattc ccaaagtgta ggaatgtcca atcgtactta ctgttttcca
    54421 aaaagctttt taaatcatca atattatcgt cggctgaaat ggtgacaacc tccagatgct
    54481 tcttgtactg cttgcttatt ttttcaagct gaacaaactc ctttaaacaa gtgtagcttg
    54541 tggtggtgca aaagttcagg taaacatact tgcccttgaa tctatcaagc gaaacctcac
    54601 gaccattaac atcgttgagc gaaaaaggtg caggaacaaa acccggtaaa agcttggtaa
    54661 tcctgttcct gatattctcc gcaactatca catgttccgg tatggtggtg gttctgtaaa
    54721 tggagtcaag aatggtaagc agagccgatc gggagaaatt atcgctgaaa aactcatcgt
    54781 gcaacgattt aagaattacc atctccatca gggtatcgtt actaagcaca tcgtcggtgg
    54841 caagggtacg cttaagcctg cccaaactct tgtagcgcga aatatcatca aaaatggcat
    54901 tcccctgcga tgttcttgaa aagaacaaaa agtacttgtc gtaaaccaga ttgaacaatt
    54961 ccatgtatgc cgggttacgg tacaatatag gcctattcag gaacagctct tttgatgtgg
    55021 cagtagattt ttttatgaag gtgatttgcc tgagcaaacc gtacctgtac tcacggtaag
    55081 catcgaaaaa ctggttgccg gttccggcaa acttttcctc aattctggtt attaacgaat
    55141 cgatattatg ctgacggcca cggtatgcat cctgcacaat ctgatcaaaa tcttggttga
    55201 agcgcaagtc gaatgcattt atcaggaagt tgatatcgtt cctatcaaca ttttttatgc
    55261 ccacctgcaa atcggtttca cggaaaaaag gattgagtcg ttgtgcctcg gttttatcct
    55321 cgcgaggggg aagcactagc tcataccgtt taccaggctc ggcaaagaaa taaatcctat
    55381 aaactccaag gtgggcaaaa acaaagctgg tttcaagcac tttaagcttt gcgttaaact
    55441 tgccatcgcg ctcaacctta cacctaccaa cctcgtattc ggtttcagtt atgtaatcgg
    55501 aataggtata aaatattata tcggagccct tgtattcagc agcattgcca aaaatcatgg
    55561 cctcctgggc gccgatttta cactcaacgc caataaacag cagtaagaat aataaaagaa
    55621 acttaaattt agccataagt ttaaagataa tccttgatgt taatggctga tgggataaac
    55681 tgcgatgggt cgccattatg caaaagaata tcgcggataa tggttgagtt aacgggtgtg
    55741 tgctggggtg cagtaagcat gaataccgtt tcaatattag ggtgcattag cttatttacc
    55801 tgcgcaatgg cacgttcaaa ctcaaagtcg gctgaagtac gtagtccccg taggatgtac
    55861 cgaacgccaa gcctattgca aagatctacg gtaaggccct cgtagctaat cacctgaaca
    55921 cgtggttctt gagcaaacac cttttggatc atggcaatcc ttttctcaat gctgaaaaaa
    55981 gcctttttat tggcgttata gccaatagca actatcacct tatcgaaaag ggataatgat
    56041 cggtaaacaa ttgattcgtg accgactgta ataggatcga acgaacccgg aaacagggca
    56101 actttttcca tggctaagtt tatttagctc cagcaatttt catacaatta taggtaaaat
    56161 tgacgactta atagttaata aacaaaaaaa gacccttaat tcagggtctt ttcatgcatt
    56221 atattcacgg ctaatcaata ttcataggtt tatcgacctt ctcgtttagc aaagctaaat
    56281 caagcacctc gtggatgttg ctaacgtaat ggaatttcaa ccctttaaca tatgtagcat
    56341 caatctcgga aacatccttg cgattatcgg ccgaaagaat gatatcggta atgttggcac
    56401 gtttagctgc aagtattttc tccttgatac cacctactgg cagtaccttg cctctaagtg
    56461 ttatctcccc agtcattgct acgtttgccc taactttacg ctgggtaaat gccgatgcta
    56521 gtgaggtcac catcgtaata ccagccgaag gcccatcctt tgggattgcc ccttcgggta
    56581 catggatgtg tacattccat ttctcaaaaa cctcgtgttt aattccaaga taatcggcgt
    56641 gcgattttat atattcaagg gccagaatgg ccgactcttt cattacctcg cccagattac
    56701 ccgtaagggt gagattacct ttgccgcggc taagactggt ttccacaaaa agtatttcgc
    56761 caccaacttc agtccaagcc agaccggtaa caacaccagc aatatcgtta ccttggtaaa
    56821 gttcctttgt atagcgcgga actccaagaa tcttttcaat atccttattg gttagcttgg
    56881 gattatactt ttgctccgag gcaatcatac gggcagcata gcgaaccaac tttgcaattt
    56941 tctggttcag gtttcgaacg ccgctctccc gggtgtattc ctggattacc ttggccaata
    57001 ctttatcgct caactccaag gcatcactat tcaagccatg ctccttgagt tggttgggca
    57061 gcaaatggcg cttagcaatc tcgacctttt cctccaagct gtacccgcta acctcaatca
    57121 tttccatacg gtcgcgcagc gcaggattta tggtagccac ggtattggca gtggtaataa
    57181 acagcacctt tgaaagatca tattccacct ccacaaagtt atcgtggaat gtgctattct
    57241 gctcggggtc gagcacttcc agcaaagccg aggcgggatc gccatggaaa tctttgccca
    57301 ccttatcaat ctcatcgagg atgatgacag gatttgacga ttttaccttt ttcaggcttt
    57361 gaattatgcg gccgggcatg gcgccaatgt acgttttgcg gtgaccacga atctcagcct
    57421 catcgtgcat acctcctaac gagatacgag catactttcg acccaatgct ctggcaaccg
    57481 acttgccaag cgaggtttta cccactcccg gaggtccgta taagcatagg attggcgcct
    57541 ttaggtcgcc tttaagtttg agcacagcca aatgctccag gatacgctcc tttacctgct
    57601 ctaggccaaa gtggtcttca tcgaggatat ttttggcatg atcaaggttg aaattgtcat
    57661 tggtgtactc attccacggc aagtcgagca gtagctgaac gtagttcagc tgaaccgaat
    57721 actccccggc catggggttc atacggttga gtttgttgac ctccttatca aaatgttcag
    57781 caacttcctt actccacttt ttggccttag cgcgctcctt gagctcattt atctcctgct
    57841 caatggggtt tcctcccagc tcatcctgaa tggttttcat ctgctgctga aggaaatact
    57901 cacgctgctg ctggtcaaga tcgtgcttaa ccttgttctg aatatcgtgt ttcaactcaa
    57961 gcatttgcag ctcacgggtt aatattcgca gcagctgctc agcccgtctc ttaaggtcgt
    58021 gctcttcaag caatgcttgt ttttcatcag tcttaatatc gctatttgcc gatataaagt
    58081 tcacaagcat tttggggtta tcaatattct ttatggcaaa cgaagcctcc ttgggtatat
    58141 ttggcgaaag atgtattatt cgaatggtga gatcccttat tgattcggtt aaagctatga
    58201 actctgtatc gtcctcgttt ggtaaaagct cagccagttc cagaacgcga gccataaagt
    58261 atggctcctc acttacaaat tggtcgacac ggaatcgcga taatccctga agtataactg
    58321 acgagttgcc atcgggtagc tcaagcacct taataacctg agcaatggtg ccaaccttaa
    58381 aaagatcgcc tgatgaggga tcctcaatac gggcatcgac ttgcgagagc accccaatta
    58441 ttttcttctt tggtggtaga cttgcaatta ggtttagcga tttctcccgc ccaacggtaa
    58501 ttggcatcaa tacaccggga aagagaacag tgttacgcaa tgccattatg ggtagcacat
    58561 cggggatatc tgcctttttg acctcgggca cttcgccctc tgccaaaatg ggtataaagt
    58621 ttacttctga atcgttagta tcgtccattt gctgatttag tattgatagt ccaaactttt
    58681 tcttcatttt atctcctctc aattttctga caatatgaca cattaaaggg taaaactaac
    58741 ccagcttact atatggtcaa ttgctatgcc aaaaagtagt tgatggttgt tggtattaaa
    58801 accttgaacc cctcagagcg gaacaggcca ttaaaccctg aatatctacc ctttatcctt
    58861 tatcctttaa cctttaccct ttatccttta ccctttatcc ttaatacatc ctgtattctc
    58921 gggttagctc aaaaacctta agtaggattt ccttgtcgat atcggtaaat ggcaaatcgc
    58981 gacgcgacat cataatccgg gcggtttcaa aagctttttg gggttgatag gttgtaaagc
    59041 cggctgcccc tccccacgaa aagcaaggga taaaatttcg ggggaaacca tcgccaaaaa
    59101 tgttggcact tacaccaacc actgtgccgg tattaaacat agtatttatg ccacattttg
    59161 aatggtcgcc cattattagc ccacagaact gcaatccggt atcaacaaat cccttttttc
    59221 ggtagctcca aagctttaca gaagcgtaat tgtttttgag gtttgagtta ttggtatcgg
    59281 cacccagatt acaccattcg cctattaccg aattaccgat aaaaccatcg tgagccttgt
    59341 ttgaaaaagc aaaaaacacg ctgttattaa cctcgccgcc aaccttgcag tatggaccta
    59401 cagtggtagg gccatatatt tttgcaccca ttttcagcgc tgaatgctca ccaagggcaa
    59461 acgggccacg cactatggaa ccctccatta cttcggcatc cttccctatg tatatcggac
    59521 cagtttcggc attaagaatt gcgcactcca cgcgagcacc ctcctcaata aaaacattat
    59581 cagggtttaa aacagtattg gtagctgaaa ttggttgcga cttccgcccc ttagtgataa
    59641 ggctataatc gcgaacaatg gcttcgccat tgtttcggaa aatatcccag ggatacttga
    59701 cctcgagaat atcatccgga tactcaaacg tcttctcaaa caagcccgct gcgggtaaag
    59761 tgtccactgt gctattaagc attgccgcta taagcgtgtc gcccttaaca attgcctggt
    59821 taggtttgag cgctcttatt ttatcaacca aattgctatc ggggcaaata cctccattta
    59881 ccaacaaaac aggttcccct ttgggggcat tgggatattt ggtctgaagg taatcctgcg
    59941 ttaaatagtg aaccgatgcc ccggttaaca ttgaccactt ttgggatatg gtaagaatac
    60001 ccatgcggat ttcagccacc ggacgggtaa aggttaaagg cagtaaatcg tcgcgccttt
    60061 ggtcatcaaa aagaactatg gtgttaatca tataaatcga tttattttga acaaagttac
    60121 caaaaaaagt aaccctttta aaacaaaaaa agctcttata cctaagagct tttcctgaat
    60181 tatttgagca acattacttt ttgttatcca tgtgcttttt gtagcggttc atgaacttat
    60241 ccacacgacc ggcggtgtca accagcttaa ccttaccggt gtagaatggg tgtgaagtgt
    60301 tcgaaatttc aacctttacc aaaggatact caactccatc gatagtaatg gtgtccttgg
    60361 tgttaacagc cgacttggtg ataaatacat gctcattgga tatgtccttg aatgccacca
    60421 gacggtaatt ttccggatgt attccctttt tcattgtatt ataaatttgt ttacttccaa
    60481 cttcggttta atgggctgca aagttatcaa cttatattta aatatcaaac tattgcagct
    60541 aaaaaatgat ttatccggat aagttttaat ttcgcctgtt cagtttggca agcagtttaa
    60601 gaatctcaat gtaaagccag ataagggtaa ccatcaagcc aaatgctcca taccactcca
    60661 tgaacttggg tgcacccgat gctgaagccc tatcaataaa atcgagatcg ataatgatat
    60721 taagcgctgc aacaccaact actattaggc taaagatgat tccaaaggtt ccgctaccat
    60781 gaataaaacc caagcttatg ccaaagaaac tgagtacaaa ggaaataaag taggttaatg
    60841 caatagcgcc agtggctgct aaaaccatca ccttaaattt tcctgtagct ctgattgttc
    60901 cgctacggta agccaatagc atcaggaaga aaacggctag ggttaaccca attgcctgca
    60961 ttattatacc agcaccatac gatgatgcaa agtaggccga tattccacca aggaaaatcc
    61021 cctcaagcaa ggcataaacc ggagcagtgt aaggtgaagt tttgggcgca aatatcacca
    61081 ccaacgatgt aataaaaccg cctattcctc caacaaacat ccacggaaca accgatgcag
    61141 ggttgacact ctcgaaaaag atacgccagg tataagctgc tcccagaatt accagcgcaa
    61201 gcatcagcaa tgccttattc aaagttccct gaagtgtcat aacctcagca cccgaagcat
    61261 aaccagcttt tgaaaatatc ctgtccgata gggcaggatt tgacgattta gttaagttca
    61321 tacaaatacc ttgtttaaaa ttcaatacgc attattcaaa aattatgccg tgcaaaagta
    61381 gcagattatt cggttaattc aaaaagtagc tcattaccaa cccatgcagc cagctcgttc
    61441 tcggactcgg tcatcctact ggctgtttcc gaaacccttg cagccagttc aggactgcgt
    61501 ttagcaattt cgcgtaaggc gaactgaact gccgactggg ttaaatcggt catctcctcg
    61561 gccatattct caatgatgcc aatataaggt tcaagcaaac tctctttaat ggctgtatcg
    61621 gatgccttac gtgcaataat tagcaaacct gctttacgca aaagctcatc gttgcttagg
    61681 caccattcaa tgctacgcga aagcgcaaat tccgatttcc agaacaagtt accgcaaacc
    61741 tgttccacaa tttcggggtt ggtaaactcc gcagcccact catcaatctg ctcacctgtc
    61801 aatctttcag ggtcggcaat caggcttgcc agtattttac actccctgat atcttcggca
    61861 taaagttcta acgccaggtc atggtcgccc ttatagggct ttgccaactc cttgagttca
    61921 ggaataggaa cgccatagtt tactttgtaa ataataccgg ccatacgcat gttctctgcc
    61981 acttcgccac tagccctgtg tattatctgc gattttatct ccaagacctt ttccaacgtt
    62041 gccataagtt ttccaatttt gctcaaaatt acactgaaaa agtgcagatt caaagctttg
    62101 ggggaaatag ttcagcctaa gtgccatgaa ttttgaggaa tgggtcggaa tgaatcggaa
    62161 tgagtcggat tagacgaata tgatgaagtt agaggaagtt taagttcgtt aaacgtgaaa
    62221 agtgaaaagt taaaagaaga attgagcaga taaataaata catcaggagt ccgacaaaag
    62281 tttttaggaa ccaaaaactc gtacttattc aattaccgcg tagcggttag actgttgtaa
    62341 tagaatagaa aggagcatac tttatattcc tcttataaga taaaggtttt taatagaaaa
    62401 tcaatgtcgt ttagttaaca aaaatattca tgcctgtctt cctgagcggc ctgtcccgcc
    62461 ctagcgggga gcgaaggatc tacaatcgac ccattgagac gtttcgcttg cgctcaacat
    62521 ggcaatagtt ccacgaacaa cgaacaacgc ttccagcccc gtaggggcgt aatatttgta
    62581 actccatggc tactccctca tgtgttgcga tgcacgcaat acattttcca aagagcaagg
    62641 gggaagcgtt gcatcgcaac ggaaaagcat tacaaagagt tatttaaacg ttctttcttg
    62701 tcttgataca agaaagaacc aaagaaaatc aaggctgaaa agcccgaccc gatgggtacc
    62761 ccgcgggtgg aactgccacg cgatactatt cgccacgcct gcggcgtgac tcatgtggta
    62821 tcgcttacaa aacccatccc cacgttccac ccccgaccca ttgccgggtc aggcttttat
    62881 gccgctgctt acgccattcc ttgctggtta aggaatctca ttgattaata aaggggatga
    62941 agggacaaag agatgcttcg gatgggctca gcatgacaac caaatcatga aacgagcaac
    63001 aacaggtatc acagagttac acagtgtaac acagtattac acagaatagc attcacgaat
    63061 aacgatacaa gaacaaccaa cgccgtcacc agccccgtag gggtgtaata tttgtaacac
    63121 catgattatt ccaacatgta aagcgatgca cgtaatgcat atgccaaaaa gcaagggtga
    63181 ggcgttgcat cgcaacggaa aagcattaca aagagttatt taaacgttct ttcttgtctt
    63241 gatacaagaa agaaccaaag aaaatcaagg ctgaaaagcc cgacccgatg ggtaccccgc
    63301 gggtggaact gccacgcgat actattcgcc acgcctgcgg cgtgactcat gtggtatcgc
    63361 ttacaaaacc catccccacg ttccaccccc gacccattgc cgggtcaggc ttttatgccg
    63421 ctgcttacgc cattccttgc tggttaagga atctcattga ttaataaagg ggatgaaggg
    63481 acaaagagat gcttcgacaa agtctattcc tataaatcgg gactcagcac gattgcgtta
    63541 tttttcgctt gcgcgcaaca ttacaaataa ccttaactaa acaacattga atcccagttc
    63601 aatatttaag aacgcaacct taacaaggtg cttgattggt tactcccact taattgtttt
    63661 tacccttctg ccagctttac aggaaaaacc aagaaaacgc aaaaacggaa agcgttaaca
    63721 gggaataggc caaaaatatt tttactggca catatgaaac taaagaaatt tttaactggt
    63781 aggagttgta tgccataagc aacacttttt caacggcata aacaattacc gtagctgctg
    63841 ctactccatt tattccaaaa tacttaacca gtaaaaggct cagaatgata ttcatgatca
    63901 tttcagcaat tgaaatggat agtacagcca cgtttttacg aaatccctgg gtaaccgttt
    63961 gcgggaatat aaccctgctt aatacaatta agcagtatat cataaaaaca ttagcacttt
    64021 cctggaactc agcaccaaaa acaaggggga atagccatcg ggatagaagg attgcagcaa
    64081 ttgaggtagg gaaaagaatg tgcatcaaca ttgaactttc cagtttaaga ttcaagagag
    64141 tatttcccag cctttgcctg tcggcaaaat cggccagcat ggagttgctt aacgatgctg
    64201 caagaattgt agccaatggg atttcgcgcg ctccaaaacg gtaaaccgca aaggttgatg
    64261 aatcgtaata gtatccaata attaattgat cgatatataa gcctgagctg ctaataagga
    64321 atttcaagga aagcgggatg ccatcgctta ggtaatcctt taaaaaatcg acattaactc
    64381 tgaattttga attacggact aaaagcgata ttaaccatat aaaacgaact gccgatacca
    64441 ctatcaaacc caatacagca aatgccacat ctaaaccaaa caatacaggc aaaaccacaa
    64501 ggattatctg aatgccatag gtaatgacgc catacgatag catggcctta taatttccgt
    64561 gcagaaagta aatgtattcg gccattgttg aaggggtgct cagaagcatg taaagtagca
    64621 tcaaggggaa aaggctatga ttaatggctg caaaaccagt aatagttgcg gtaaaaactg
    64681 tagcaaacag gcaagccaaa acagtaagcc caaaaatgac cataaaggca ttaaacaaac
    64741 ggtcatcttt tgaaatgtcg ttactacggg tataaagagg aagcaatgct tgaattagcc
    64801 ctgtaaccca aaatgtagta agtagcccgc taataaagag taccgcctca tagtttccta
    64861 ttgcagtttt tccaaagcca agccttgcca tgactatccc aatggtgagc atggctacaa
    64921 aacgcattac ctgaaaaact tgcaatgcgc gtgtagaatt tgatacaagg cctaacataa
    64981 ttataactta ctaatgcgaa ggtattaaaa ttgatgtcaa tacagcgttt aatcaaatta
    65041 cttttgaaca tttttgcatt tagaaaaaat gtttatacct ttgcagccgc aattgacatg
    65101 gtggatgtag ctcagctggt tagagcacca gattgtggat ctgggggtcg tgggttcaag
    65161 tcccatcttc cacccaaacc cggccaaaag ccggtttttt tattttaata gatttaaaat
    65221 tgcatgtaaa ttattttcaa attttctgcc agtcatttta tcctcttcaa aacttctgaa
    65281 catactccaa gaggaaccgc ttcagctgaa aacgctattc cattctagct taaacatttc
    65341 tttcatttcc ccttgacttt tccctatcct aaaggtaaat ttggggaaat accaaaccgt
    65401 tagcataatg tataatctgc ttaaccgtag taaaaagctt tgtgctttta cgctaacagt
    65461 tttaatagta accgaaacat cgtgtaacgg acagaatatg aataacccta acgatttcga
    65521 tagacaacca gctgtagcag gacaatttta tcccggcaca aaggctcagc tggaaaagga
    65581 ggtgaaatct tacttcgata gtgcacaaaa ggttttggat gctcagcccc tagccctaat
    65641 tgtacctcat gccggttacg ttttttccgg tcaggtggct gcaagcgggt ataggcaaat
    65701 tgacagggat aaaaagttca agcatatctt cctgatagga tcgagccaca ccatgttttt
    65761 tacaggagca gccgtttaca ccaagggtgc atttattacg ccactaggca gggttcctat
    65821 tgaccctttg gcaaacgaac tggttcaaaa ccataaggtg tttacggatg atgtaaaacc
    65881 acatgtcaag gagcatagcc ttgaggttca attgccattt ctgcaatact ggctaaaaaa
    65941 cgatttttcc attatcccca taattattgg tggcgaatcg caagccactg ttaccctttt
    66001 ggccgaagca ttagaaccat accttaaccc cgataatctt ttcataatca gttccgactt
    66061 ctcacactac ccctcatacg aggatgcccg ccacaccgac gacgatatgg cccatgccat
    66121 tgtctctaac tcgcctgtag agttcatgaa agccaaaatc cgaaacgaga atgctggtat
    66181 cgacgacctg gcaacagcca tgtgtggatg gacatcgggg cttacgcttt tgaaaatcac
    66241 tgaggggaaa cctggcatca cctacaaaac aattatgtat agaaactccg gcgattcgcc
    66301 ttacggcggc aaggataggg ttgtaggcta ctgggctatt gctgccattc aacagcaagc
    66361 caaaactgta gacttcaacc taaccgacaa cgataaacgc gagctactca aacttgcacg
    66421 gaaaaccatc accgaatacc tttccgggaa aacccccaca ccacccgatg agtccaaact
    66481 atcatcaact ctaaaaacca atgccggtgc ttttgtaacc ctgcacaacc acggtaagtt
    66541 acgtggatgc attggaaatt tttccaccac aaccccactc taccgtgtag taatggccat
    66601 ggccatatcg gcagcaactg aggattaccg ttttgaatcg gtaacagccg acgagttgaa
    66661 cgatattgat attgagattt cggttcttac cccaatgaaa cgaataagcg acattaagga
    66721 gatagaactg ggtaaacacg gaatatatat caaaaaagga ggtcgctccg gaacgttttt
    66781 gccacaggtg gcaaaggaaa cccgatggtc gcttgaagaa ttccttggcc actgcgcacg
    66841 cgacaaggct ggtattgggt gggatggctg gcgcgatgcc gaaatattta cctacgaggc
    66901 cattgttttc gatgagaagg aactgggctt aaagtagatt gctgtccatg agttcaccct
    66961 acctcctgat tcccatttcg ttagccatca ccatatatta tctacttggc agcctaatgg
    67021 tgcaagcagg cattatatct ttttcaaccc atcggaagtt ctggaacttt gctctgctat
    67081 ttgtattcct tggctgtgcc attcttggaa ttctactaat aatcaatata aactataagc
    67141 tggggtggag ttttgttaag gtcatgctac agtggcacgt tcagcttggg attgccatga
    67201 gcgtggtggc attcattcat accctttggc acaggggata cttcagtaaa taccttttgt
    67261 ttaggggtag gcaaaccaaa acgacaacgg ttaatacaaa gcagcaactt gtaaagtcag
    67321 cttttgcgct tggactactc tcggtatcgc ttcaggtact gctgattagg cagttcacta
    67381 aggtattcca ggggaacgag ttcctaataa catggataat aggtatatgg atgctactgt
    67441 cggctgcggg tgccattgcc ggaagccgaa cggctaacgg gataaacccc caaaagttaa
    67501 cccgaatggc actagggttt atgtttcttt cagccataat ttttattttc ttatcgggcg
    67561 aaatcaggca aacctttttc ccgtcgggag tgcttattcc accttaccat gttatagtga
    67621 tgattgtagt aatgatgact cccactgctt tcccatcggg catggtttat gccttgctca
    67681 ccaaacaaat aaaatcggaa tacccatcca tttatgctta cgaagcatta ggaagtttgg
    67741 cagggggact tttgctatcg cttgtaatta tctggatttt taatacatac cttgcagcaa
    67801 ttgcggttgg ttttttagct agtttaatca tagcctaccc ccttaaaaat attgccagac
    67861 ttgctattcc aatgtcgctt ttaacaattg gcatatcagt aaaaacatta aattttgaca
    67921 ttaaagcgga gtcgatgctt tttccggggc agaaggtact atcggtaacc gattcgccct
    67981 atggaagtat aaccgtgacc ggttcggagg atcagttcaa ttttttcgaa aatggaacaa
    68041 tgctttttgg cacgcaaaat accatttact gcgaggaagt agtgcattac accatatcgc
    68101 aacgctacat gcccagtaag gttttactgg tatcgggagg ttatgcgggt ctgattgatg
    68161 agctgaaaaa gtataagcaa gtaaaaaaag tagattatgt agaacctaat ccccatcttt
    68221 taaggatgag tggaaactat tgtaaggttg atttcccgga caatactaaa attatcaata
    68281 gtgacattcg tggttttctg agaaaatcat cggaaaagta cgacattgca attatcacaa
    68341 cgcccgagcc aacatcgctt gaacaaaacc gtttctttac cattgaattc cttgaacttt
    68401 taaagaaaca cttaacagca gatggggttt taagtgttag cctttcaggt attgggaact
    68461 acccatcggc accaaggcaa aaggcctaca cgtcgatagc agctacgttt aagagcgttt
    68521 tccgaaatgt agaggtaata agcggcgagc gcgattacct gcttgcatcg gatagcacta
    68581 tacgtattga tatggctcaa ttactaagcg agcaaaatat tggcgaagcc aacctatacg
    68641 tacgccccga ttacatcaac gatgagcaca ttgctcagcg taaccatttc tttcatgagc
    68701 aaacccaatc aaccctgaaa ataaacaccg ataatcatcc ttggccggta ttgcaaaaca
    68761 cgctagggta cctatcgatg tttggcgata agatttggct acttatggtc gttggagccg
    68821 tccttttact tatccctttc tttgttacta actaccacct gcgcgcgatg tacgctgtag
    68881 gatttgccgg atcggctatc caaacccttt acctattaac tctacaaatt gcagcaggct
    68941 tactttacgg cacgcttggc gcaatgattg cccttttcat gggaggacta gcgctaggct
    69001 caatggcata cgggaaggta atcagtataa aacctaaaca cataaaaatt tcattaatcg
    69061 gttcatttat tattcttttt gcccttttta taactatgat gcatgtggaa atttggctat
    69121 taatagccat tgtaagtgca ggaatactca ctgcttcgtt tagtgttggc tacctgtata
    69181 ttagtattac cgagaattca aactctaacc ccaaccaacc tgccaaaacc tatgctgccg
    69241 accttatggg atcggctgct ggcattgtaa ttgttacact tttgctaata ccatcaattg
    69301 gatttctgtc aagcattgcg gtattattgg ttttcattgg catttaccta tttcttaaag
    69361 tttaaaaaac tcacaccatg gaaaagatat cgcgccgcga attcctgaag cggagtcttt
    69421 tagcaaccgg aggttttatg gccctgcgat acttaagcct atcggcattt gggttggacg
    69481 ataaaccctg gaagtggagc cgcgaggcaa tgttttacac acccactccg cgcggtatgc
    69541 gttgcaacct ttgccccaat gagtgcaata ttcgtcccgg agaaaccagc cagtgccgca
    69601 accgggttaa ctacaacgga aagctataca ccattgccta cggtaatccg tgcgccgtgc
    69661 atatcgatcc cattgagaag aaacctcttt accattacta cccgggaagc aaatcgtttt
    69721 ccattgcaac ggctggttgt aactttggtt gcctaaactg tcagaactgg gagatctcgc
    69781 aaagcagtcc caaggatacc cgaaatgcag agctattccc cgaacaggtg gttgaacagg
    69841 ccaaacgcaa tggatgcaaa agcatagcat acacctactc agagccaatt actttttacg
    69901 aatacatgta tgaaaccgct ggaattgctc agaaaaaggg cataggcaat gtactagtat
    69961 cgaacggtta cataaacgag gaaccgctac gcgaactcgc aaaaaggatt gacgctgcca
    70021 acatcgacct gaaatcgttt agtaatgaaa tctacctgaa gcttaatgca ggaaccctgg
    70081 agcctattct taacacctta cgcatactac tcgagatgaa cgtttgggtt gaaataacca
    70141 acctggttat acccacatgg accgatgatt atgccatgat acgtaagatg tgccagtggc
    70201 tggcaagtaa tggattttcc gaagtgccgt tgcattttag ccggttcttc ccgttataca
    70261 aacttaccca actcccacaa acaccaaaat caacgctaat taaggcaaga gagattgccc
    70321 aatcgtgtgg gttgaaatat gtttacatag gcaatacagc tctgcccgga tcgggcgata
    70381 ccatttgccc atcatgcggt aaggtggttg tggaacgcaa gggatacaat gtagcacttg
    70441 ttaacattaa caatgggaaa tgcggctggt gtggagcaaa aattaatggg cgatggtcat
    70501 agctatttca acgcactgct ccccttgctc actcgcgtct gcagacgcgt acggcatacg
    70561 caactcattg ctggtgcggt cttatagccc gtgataaata catactacac ttttacttat
    70621 tcaagctgtg cctcattgaa aacaccttta aaataaccgc acgaattggc tttgccgaac
    70681 tacaaccgca cggattacaa ccgaccgaag ggagctcacc gaaggtaaat ccgcgcgagc
    70741 ggccgaggaa tcgtgcggta gcggggctat tttgtgccat tattcccaaa caacttctcg
    70801 tactcctgtt tgctataaag tagaccattt acatagtaac cataatcatt acacttatta
    70861 aactcctcct ttgttgggta tggcttgtat attattgaaa gtttactaca ccctaccggg
    70921 taaattatta gaagactatc gccctgttta aattttttga cactaaaaga tttcttttca
    70981 agggttgttc gttttgttac cctcatataa aatgattttt tatcactttt tttagtctta
    71041 agatataaac cagcacctga cgaaccccta aaagtattat gaacatatac tttacggact
    71101 tggcatgcat ttcgcttaat gtattctata ccatttagtt cattaaggat atcgcaagga
    71161 ataaacaata agaacggaat aagccccaca aatacaaaac gatttttctt gatttttaaa
    71221 ataagagaaa ataaaaatcc aactatcgat atgataaaaa ttaccattaa agtacctata
    71281 ctgaataaaa agcaaggatt ccatccaggg tagtaatagc cactgcatac taatacgaga
    71341 taaattaaaa caataaaata tactgaaata aataaaatac gaattttact gttcatctga
    71401 aataattata agttttgagt cattatttac ttcataatat ttttctgctt tatctttaaa
    71461 ataatcaccg cacggattac aaatccgagc aagcggagtt ttctttctta taaaaattcc
    71521 aatttttata aaaatagaaa aatattcaaa cagatacaag aaatttagta aaaaaaataa
    71581 tcttcaaatt taaacaattt ttttctaata atttcatagt ttttaaaaca aagacacatg
    71641 gttttaaatt caccattatc ttttgctact tgatatatat atttttcacc cgcttttatt
    71701 attttctgta attcattaat atttatttcc aattcattcc gttcttcttc tgtagtatta
    71761 tttaaaataa aaaaataccc atcacaatat ctatcgtact gtgatatcgg gtatgttctt
    71821 tttaataatt cgaatttatg cattaattga gatagtattt cataccatat ctaataaaat
    71881 ggacaccgtc ttttttatgc aagtctttgt atacattcaa tacgactaaa ctgtccattt
    71941 tgtattgcag ttcagttcta tcgttactca ttgaatatat tatactatca ttatctttat
    72001 ctttcacatg tacttttgca taatacgtaa tagtaccatc aggattcttt ttttcatcaa
    72061 gagtaccata ataacgccta ctaattgctt ctaatgtaaa tcctgctttt gcaacaaata
    72121 tttgtattat tttaaatttt ttctcaaatt cttctcttgt catatgcaat atttttttat
    72181 atatattacg aattgttgaa atttttgtta caaaaaacac aatttttttt tacaattaat
    72241 ttaaatattg acatgcggag agtgagagat tcgaactctc gatacgtttt cacgtactgt
    72301 aatttagcaa actactggtt taaaccactc acccaactct ccccgctcgc gcggatttgc
    72361 aatccgtggg gtaacattaa catttcaccc ccactgctgg cgcggacttg tagtacgtga
    72421 caattacttc ctacacttta ttaccctcgc tcccgctcgt ttttaaacgc gtgcggcata
    72481 cgccgctccg cccggtagcc ttcagtaaat ttagtaaaaa atcccttgtt cagtcacgta
    72541 cacaaattct tttgtccggc tgaagtgctg cctttggctt ggactccgcc caactccctc
    72601 gcttgcacgc gtccgcaggc gcgcgccaaa gagggggaat aactcgggat gattaggaat
    72661 tttcgaaata aacagaattc gatggagtta gaaggagtta gggtaagttt gaagaattgt
    72721 ggaaaagtga gcgattaaac tcaatcttaa gggtttttgt aaaaccatat aaatgtaata
    72781 ggtttgcaga ttattttgcg ggaatggaat ttgaactcta tacactacaa aacgggatac
    72841 gacttgccca caaacgaacc gattcgccag tggcatactg ctgcataatg gtaaataccg
    72901 gcacacgcga cgagttcgat catgagcatg gcctagccca ttttattgag catgtaattt
    72961 ttaagggaac aaaaaaacgc aaggcatttc acatcatgag ccgcatggag gatgtgggtg
    73021 gcgacctgaa tgcttacaca tccaaggagg aaacagtaat tcatgccacg ttcctcaagc
    73081 aagatttcgc ccgcgcagta gagctactgg ccgacatcat gttccatagc actttccctg
    73141 agaaagaact caagcgggaa aaggaagtaa ttgttgatga gataaactca taccacgatt
    73201 cgccttccga attaattttt gatgattttg aggagttaat tttccccaac cacccctttg
    73261 ggcgaaatat tttaggtaca aaaaagcagc ttgggaagtt taccagaaaa cacattgtcg
    73321 attttatggg acgaacctat aacaccgatg aaatggtttt ctgctcaatt ggaaacatcc
    73381 cttttaatcg aatagtaaag cttgccgaaa agtattttgg tggcatacac ccaaacaccc
    73441 gtaactacaa aaggcttcct attaccgaat acttaccaca aagaaaaacc gttaagaaat
    73501 caacctacca atcgcactgc attgttggca ctactgcgta cgacctgcat aacccaaacc
    73561 gtattgccat gcacctggta aacaacatcc ttggaggccc gggaatgaac tcaaggctca
    73621 acctggcgct ccgtgagcgg catggcttag cctacaatgt ggaatcgtcg tacacgccat
    73681 acagcgatac cggtatattt accatttact ttggcaccga taaggctgat accgataagg
    73741 caattgacct tattcttcag gaactggatg gcctacgaaa taaacggatg ggtatactgc
    73801 agctctccaa agctaaaaaa cagcttatag gccaactggc cattggtgct gaaagcgggg
    73861 aaaacctcat gatttccacg gccaaaagca ttttggttta cgaacgcccc gataacattc
    73921 agaacgtatt taaaaggatt gaggagataa cttcgttgga ggttatggag attgccaatg
    73981 aaatacttag ccccaatagg ctatcgatgc taatatacaa gtagccatgt ataccacaaa
    74041 ccatgagatt gacaaatacc tcgaaagcca caccaccccg gtggatgagg tgctccacga
    74101 gctaacccgc catacatacc ttaccaccta taacccacgc atgataagcg gccccgttca
    74161 gggtaaattc attgagatga tttgccagat ggtacgccca aatcgtatac ttgagattgg
    74221 tacatttaca ggttactcaa ccatttgcat ggcaaaagcg cttccagcag gcaaatatat
    74281 cgacaccatt gaggtaaacg atgagctgca ggaaaccatt gaacattata ctgctaaagc
    74341 aggggtctcc agcaaggtta accttatcat tggcaacgcc cttacagtaa tccccggcct
    74401 cccccacaca tacgatttga tatacattga tggtgagaaa aaggagtatc cacagtactt
    74461 gaacttatgt acagaaaaat taaacgctgg tggattcctg atagccgata atgttttatg
    74521 gaatggcaag gtagctgacc caacatgcac cgacgagcaa acaaaagcaa tccgacagtt
    74581 caacaaaatg gttcaggaaa acaccaccct tgaaaatgta ttgctgccaa ttcgtgatgg
    74641 aatgatgatt gttagaaaaa aattctgata attttgttaa accttttaac cattttagcg
    74701 ttaaactgat aaacactttt agcagttagc aaaatggctc aatacactat taggcaaatg
    74761 gagttgctct caggaataag agcatctacc ataaggatgt gggaaaagcg ctacaatatt
    74821 ttttcacccc aacgaaccga caccaatatc cgccgctacg acgataacga tttaaggttt
    74881 atcattaacc tatcgttact gcttaagcgc ggctttagga ttagcaagct ggcaagcatg
    74941 tcgcttgaag agctttcgga cctagcctcc aagtttgtat cggaccaccg catgggcaat
    75001 atcaaccttg aacccatgat tacagccacc atccagtaca atgaaaccga attgctaaaa
    75061 cacatcagcg aatggtccga gaagcatgga attgagtaca catttgagaa tctggttctg
    75121 ccctatctcc aaaggcttgg cgacctatgg caaaccggag caataactac aacacacgaa
    75181 cactttgcca gtaatgtaat tcggaattta cttagccacc tttaccgcac ctgcaaacaa
    75241 cccgaaattg ggaaatcaac accaatcatc ttcttcctac ccgaaggtga attccatgag
    75301 attggactac tatattttgc ctatatagcc aaaaatgcag gctataagac catttacctt
    75361 ggccaatcga caccaatcga cgatgttatt aggtgctcca aggacttagg ctgcaaaatg
    75421 gtatttacat cgctttcatc gtttgttagt gccgatatgg acgaggcctt tgaacccatg
    75481 aagaaggcct tacccgatgt ggagattatt gcaacaggac atgcttttac ggaaaaaagc
    75541 ggtccatctt accttaccta tgtttcatcg gctcaggacc tgatgaagat aattgcccga
    75601 ctcgacccaa caataattaa gtaaccaccc caaataagct aattaacgtt ataaatcccg
    75661 cccccggcgg gattttttct ttacatgcta tttagacaaa ttcattcaac gccaaccaaa
    75721 acaatcaagt tgccataaac ttatttagaa cccataaaaa gttttgtatc aattgataaa
    75781 tatcatcttg atcatcttgc acactgttta gattaattct aaatttgttt caacaaatta
    75841 attttaacta gtctaaataa aaaccaaatc atatgtcaca gcaagaattc aaccaagcac
    75901 ttatagagct ggagccaaat ctggagcgat ttgcatacag tctcacagca aaccgcgagg
    75961 atgctcgcga tttgttacag gaaacttacc ttaaagcttt aacctacaag gataagtttg
    76021 aggataacac aaatatcaag gcgtggacat ttactattat gaagaacacc ttcattaaca
    76081 actaccgcaa atcggtaaag cagagcacca cctttgatgg cagcaccaac cagttcctaa
    76141 tcaacagcaa acccgacagc tttggccccg atgccatgta ctcgcacagc gagattagta
    76201 aaaagattaa tgagctcgac gatgagttcc gcatcccctt ccagatgcac acatcgggct
    76261 acaagtacaa ggagatagcc gaaaagctaa accttaaaat tggtaccgtt aaaagccgaa
    76321 tcttcttctc caggcaaaaa ctgatgaatg ttttaaagga cttcgagtag gataattcgg
    76381 tatccgtccc gaaaagagcg ctacacaagt agcgctcttt cattttataa atgagtttac
    76441 ttacacgaac ccaaaaatat cttaaagtgg aaataatgga attgctatat ttgcagtaaa
    76501 ccataaggtg caatgcaact aaaacttatt gtaatcacat tcttactttt cccgctcaca
    76561 ttggcttcgc agccggtaat tgacgtatac tttagttccc ttgcacccga atcgaaaggc
    76621 ccatcgttta gcaaggtgga ggaagtacat gagttgctta ttcccaaaag cggaactccg
    76681 agttacttta aaatagtaaa acagtacaac tcatacggaa agattgttag cgaaaaacgt
    76741 tacaacaaag caggtggaat tatgggtgaa agccaatggg agtacaatgc aaaaggcgaa
    76801 ctcaccaaaa aggtaaacag gcagttcatg aattttaagg gatggattaa tgagcaaatc
    76861 ctgctaacct ataacgacac ctctggagta cttgaagaga tatcattgtt ttttgagaat
    76921 caacttaaac aacgggcaca agtaatcccc gacaaagacc ttaaaattac cgaagtactg
    76981 gtttttaacg aaaaaaacgt gctaaccgga aaggaacgga ttgtttatct cccccaaaac
    77041 aatactataa gggtacttag ctacaaatca aacgaacagt ttataggagc aaccacctac
    77101 ccactcgacc ctaaactgcc tgagccacct tcagccataa agcgcgagtt taactcacac
    77161 ggcgatattg tgctggaagc actccccaac agtataatgg agcaaggtta cttttacgag
    77221 tatacatacg acagctacgg gaattggact gaaaaactca cctaccaatg cagggtaaca
    77281 aagaataata aggttaggga taaaaagcta gagtacaaaa tcacacgaaa aataagctac
    77341 taacccctaa aaccttggcc ttaccccatt tgctcgccta ttgcaaatcc aaaatatatc
    77401 ttttgatgaa ttttatcaat accagtttct agcacactgc ccgatattgc aagtgcaaaa
    77461 cttgtcactt atcaatcttc tcaacaaaca tcccttaatc tattacattt taaaaacctt
    77521 aactgaattt tccgagttaa caaactgtga cttttcccta aaatggccaa agttttctgg
    77581 catcctaaca tgaaagccaa cgggtagttt caccaaaagg gattgattta taaacaagac
    77641 ccataaaaag ttcaaaaaaa atcgtttcgt atttttacga aatacaaaat atttgtatac
    77701 ttgccaagaa ttttttggag aaagatgaaa agtaattata ccgaggaaca agtaaaactg
    77761 gcgcgttacg ccaaagcgct tagccatcca gtacgggtgc agatactcca gatactctcg
    77821 cagcaaagct gctgttacag cggcgacatt gttaacgacc taccaatagc ccgcagtacg
    77881 ctatcgcagc acttaactga gctgcgcgag gcaggcctga tacaagggga gttcaatccg
    77941 cccaaaatca agtactgtat ccgccccgag gcatggaatg aagcccgtga aatattcgac
    78001 cgttttttta atagtaatat ctgggaggcg gtaaatacaa attcatgtag cacaaatgct
    78061 ggaaaatcaa aatagtaatg ttaaaccatt aaaacttcaa acaatggaaa ttaaggtttt
    78121 aggaacagga tgccccaagt gcaaggcact tgaacaggct acccagaatg caattaatga
    78181 acttggagtt caggcaacca tcacaaaggt tgaggacatt gtggatataa tgaactacgg
    78241 agttatgcgt accccagcgc ttgttattaa cgagaaggtt gttctatcgg gccgagttcc
    78301 cgatgcaaag gaactaaagg aaattataac caaaagccag cagtaagtat gaaagcgcta
    78361 aaatactttt tgcttgcagt ggcaagttta accattgtaa gctgcatggg aaactctggc
    78421 gaaaaaaaca gcattgctag cactgatggt agcggaaacc ctgttaccgt ttactacttc
    78481 catggcgaac atcgctgtcc aacttgcata gccattgaaa acgaaaccaa agccaccttt
    78541 gaaataaacc ttaaaaaaga ggcagaagaa ggaaaggtag agctacaggt tattaatgcc
    78601 gacgaaggac gaaacaagca gatttgcgaa aagtatggag tatatggttc atcgctgctg
    78661 cttgtaaagg gcgataaggt gatcaacctt actaatatgg catttggaac agcacgtcgc
    78721 aaccccgata aattcaggga ggaactatcc aacgaggtta aaaaactcat caagggttag
    78781 ccatggattt tattctggat ttcatttaca gctcaaacat tccaatacta accgctttcc
    78841 tgctagggtt ggcaacggcc ataagtcctt gccccctggc aaccaatatc accgctatag
    78901 catatatcag taaggatatt gagaaccaac acagggtatt ctacagcggc ctggtttaca
    78961 ccttaggcag agccattacc tatacagcca ttgggttgtt attctttttt ggtgcgagcc
    79021 agtttaaaat agccggcttt ttaaataagt acggtgaacg tataattgga cccgcactta
    79081 ttattattgg tctgttcatg ctcgatatca taaagattaa gctgggcggg gcaggaaaac
    79141 ttactgaaaa ctttggagaa aaagccaaaa tcgggaactt tcttagtgta ctaattatgg
    79201 gaatggcttt tgctttagcc ttttgcccct atagcggagt gctctacttt ggaatgctaa
    79261 tacctataac aatatcaaac gctaaagggt tatacttacc ggttatatat gcgcttggca
    79321 caggtttacc agttatcata tttgcatact ttattgcatt tacagtacat ggcattggga
    79381 acctatacaa taaggtaaaa acctttgagc tatggtttag gcgtgtggta gccgttgtct
    79441 ttataggcgt tggaatttac tacctaagca tttttctttt atagcaattt taaacgaacc
    79501 ttgaaatgga aaacaaaaag gaattacaag tactgttttg gataatattc ttctttgcat
    79561 ttgccttttt catgccgatt gataatgcaa ccttcagaac agccattgat gccacactag
    79621 atttaacgcg ttggtatgcc cgtgaacacg ttgtgctatg cctgctacca gctttcttta
    79681 ttgcaggggt tatttcggtt tttgtgagcc aaggctcagt attaaggtac tttggggcta
    79741 atgccaagaa atggcttgcc tacactgttg cagcagtatc gggtaccata ctggctgttt
    79801 gcagctgcac aatcctaccc cttttctcca gtatacacaa acgcggagcg ggtctagggc
    79861 cagccattgc ctttctgtac tccggccctg caatcaacat actagccatt attctcacag
    79921 cccgcatttt gggcattgaa atgggtattg cccgaactgt tggggctata gcattcagtg
    79981 taattattgg catagccatg tcactaatct accggaagga ggaacaagct aaacgcgagg
    80041 gacaaatgaa catagttcta cctcccgaaa aacgccccat gtggcaaact tcgttccact
    80101 tcttcacgct ggtactaata cttgtttttg ccaactgggg aaaacctact gccgacgata
    80161 catcgagtgc atggtattac ctatacacct ataagtggta cattaccggg tttttcggcc
    80221 tattgctcgc ttactcttta atctacatcc ttaaaataaa atggcaatgg gtagctggag
    80281 ctattggtgc cacattgttg tcagtaatac tggctaatat gtacatcgcc gatcccaaag
    80341 tactcccgct tgtacccatg ctggtaggga ttattgcttt agcctttatc accctaaagg
    80401 ataaaaatga tgacgagaat aaggaatgga caatagcctc gtggggattt gcaaagcaaa
    80461 tcatgccact gctggcagta ggggtggtta ctgccggatt tctacttgga tcgacacacg
    80521 atgggcaaac catagcaggg gttataccca acgagtggat tgcctacctg gtaggtggca
    80581 actcaatatt tgccaacttt tttgcatcca ttgtaggggc atttatgtac tttgccaccc
    80641 taaccgaggt tcccatactt caagggctac ttgcttccgg tatgggtaaa gggcctgctc
    80701 tggctctact ccttgcaggg ccatcgctat cgctacccaa catgcttgta attaggggcg
    80761 tgcttggcac aagaaaagca gtggcttatg tttcgcttgt tgtgattatg gcaaccattt
    80821 cgggccttat atatggctat ttcttttagc catgaaagat tttgataact tcatacgtaa
    80881 agtatgtatt gcaccatcac cggatgcgga tagcatctgg tatggttgca attgatgttc
    80941 taaagccaat caatttcaca tataacccga atcttcatgg ggaaatgcaa aatcagaatt
    81001 gagttttttt gatttcaatt tataaccctc aaacacaata taatgattgg gttaaccata
    81061 gtagtttctg tactcctagt ggtatcgttg cttttcgata ctcaaaaaac ctttaaagga
    81121 gttactcggg gagtaaagat gtttcttaaa ttgttaccaa ccctggttac aatgctagca
    81181 ctggttagta ttatgttctt tatcattcca aacgatactc tggtaagcct aatagggaag
    81241 gattcaggca ttaaaggcta ccttattgcg gcagtcattg gctcggtatc gcttatccca
    81301 ggatttattg cattcccact ttgcaagatc cttattgtta acggagttga atactccacg
    81361 gtcgcaatat tcatcaccac tcttacaatg gtagggatat taaccctacc agtggaagcc
    81421 aagttttttg gatggaaggt aagtatccta cggaatgttt taagttttgt agcagctata
    81481 gcaattggta ttacaatgat atttctttta tgaaaaatat aataaaatac ttccttccta
    81541 ttctttccct attatttata ggattttcat atttgtatga caatagagct ggggaacaaa
    81601 ttgccttaaa tttctggatg ttttttaagg agatgatact cttccttcca ttgatgttca
    81661 ttcttatagg actttttgat gtttgggtgc ccagagaaaa aatagaaaaa catattggtg
    81721 aggactcggg ctggaaggga acactcctgg ttattctttt ggcatcgctt caggctggcc
    81781 cactttacgg agcctttccc tttgcctatc tactctggat taagggctgt aatatacgaa
    81841 acatatttat ttaccttggg gcatactcca ccattaaaat tccgatgata accttcgaga
    81901 taggattttt gggactcaaa ttctcgctcc tgaggactct aataaccctg ccaatattca
    81961 tactcattgg ctttgccatg gaaaagtact taaaagggaa aaagtttgaa gttaaacaac
    82021 cataacaata aatttagtta tgaaaccagc actccttgca gcacttgtat ttttttcaaa
    82081 atgcattgta actgctcaaa catccgattc cacatttaaa ccaaatggca aaccaataat
    82141 tcaggttttt ggcaatttcg attacaacgc caccaacaat gcaaaaaaga cctacggatt
    82201 ttggtttggc cgtgcacact ttggctacga gtaccagttc agcaagcaaa tctcgggtaa
    82261 aattattatt gatgcaggtc gccccaccac ggttggacag attgaagtgc gggatacatc
    82321 gggcaatagc cttgatgtta ccgataaatc gaaggagggc agctactaca caatgaccct
    82381 taagttcgca tccattgagt ggaaacccaa tagcttcgtt agaatacagg ctggcggcgt
    82441 tttgcagaac cattacatta ctcaggagaa gttttggggt taccgctacg ttgcacctac
    82501 ctttcaggat aagtattacg gaatacccag tagcgattta gggataatcg gatattttac
    82561 ccttaacgag atgctcggtt ttgatattgc aataaccaac ggtgaaggct ttcggttcga
    82621 ccaggatgaa tacggcgatg ttaagatagc cggaggcatt gattttaacc cgataaaagg
    82681 gctgcaaaca cgcttttaca ccgattatac gaaatcggat aacccgctaa aaccatcaga
    82741 gcaacagcta ttctctattt ttgctgggta taaattcgat gataaattca ggattggtgc
    82801 agagtataac taccgcaaga atcatttaaa catcaataaa cataaccttt acggattatc
    82861 ggtttacggt ggcgtttccg ttgcaaagaa tgtggagcta tttggccgtt tcgaccagct
    82921 acaggccaac accataagcg gcgatgcaca aagttggtat tacagcggaa ttggaaatgc
    82981 ttacattgcg ggtattcact acaacccggt gaagggggta aacatttcac taaactatca
    83041 gggttggaaa cccctagata gtagtgttga ttttcagaat catatagtgt taagttttga
    83101 gtataaactt taattttttc tatttatgga agataacaaa aaatcaggat gcctttgtgg
    83161 cgttgacgag tacatggttt tagcctgttc gggcgcttgc gatttaggac aaataaccga
    83221 tttggttgcc cgcaaactca gagataacgg tgtacgtaag atgaattgcc ttgcagtggt
    83281 tggagcggga atagagcagt ctatcgaggc cttcaaatca agaaatattc ttatgctcga
    83341 tggctgcccc accgattgcg gtaaacgtat attggataga catggtttca ccgattacaa
    83401 atactttagg gtaaccgatt tgggttacaa aaaaggtcaa acccctgtaa ccgaagaggt
    83461 tgtaaataaa gtgtacgaaa aggttgaagt tatatactga tgcaaggtta tgaatagctc
    83521 tagatgccaa ctattaatca gggaataata ttggttttca acaccattac cagtagaaca
    83581 caacaaatca aatcaacaac tcattattga taacccactg gaattaacaa cattaattat
    83641 tcaaacgcac aagaaaatgc ttcgcccgta ttttaagaat aaagtaagaa taattttttc
    83701 gctaacttgt ttagcttgcc agcttgtgct accaactttt tcaaatgcca acacaggagg
    83761 agattcaacc cttttagaat tcaatattac cctacgcgag cgttttgagg tgtgggacgg
    83821 tatgaatgcc aaaaattacg gcgatcccaa tggagtaggc tcactaaacg ataaaatact
    83881 gtttcaacga attgttgcag gcttcacgtt caaacccaca agcaaaatcg attttgcggc
    83941 tcatattcag gactcaaggg catttggctg gtcgttaagg gactcggagt atccagagct
    84001 gttcagagta aagcagaaga atacgcaaga accctactac accatgaacc ccaacgagga
    84061 gttttttgag atttatgatt tgtatttctc ggtaaaagag atttttaagg gggtaagcct
    84121 gaaaattggc cgacaaaaga tttcgtttgg cgataaacgg atttttggcc ctggggaatg
    84181 gggaaatact ggtcgttgga cttgggatgc agttagacta tcctatagaa acaagaataa
    84241 ttcgttggat gtgtttggcg gaggcaccaa aactcacgac cccgaaaagg tggctatacc
    84301 atttaccgaa accgaacatt ggggtggcgg agcctatgcg catatccaat ttggtaaact
    84361 tgcagtggtt gagccattct acgcaattaa aacgcaaggt tcagccgatt acatccggaa
    84421 acaaagtttt tctaaacact ggattggtac aagggttttt agcgatgaag taaatcgctt
    84481 taaatacgat tttaccctaa ctcaggagtt tggacacgaa aacggaaaga ccattaacgc
    84541 ttttggcata attggtttgt tggggtatca gtttcatggc ctaccagcaa agcctactct
    84601 gtgcttgagg gaaacttatg caagtggtgg aaataatgac gatacgaggg ttcggacttt
    84661 cgaccctgct tttggggcaa aggatagcta ctacggacga atgaacatca catcttggtc
    84721 taatctcgac gaccgcgagg ttgtattgta tcttacccct tcaaagaaat ttaaaattga
    84781 aatgaactac cactttttct acctcccatc acctgacaat aaaacgctgc ttggcacaat
    84841 gaaacttcaa gccggcaaac atcaccttgg caatgagttt aacttttttg ccagttacaa
    84901 tgctggcaaa caatttcagt tcgttggggt tttcggatat ttttgggctg gcgaccttca
    84961 acccatcaat ggaacaactg ctcaaaactc taattggttt gccatacaag cctttattaa
    85021 actcgataaa gaaatacatg caaagcatta aatgaaatta taaatttagt aacctaaagt
    85081 gattgataac ctaaaaaacc aaactatgaa aatcaaacta acccaaaatg gcgaatggca
    85141 ctggcttact ggtgggatag tggtctcact actatccatt gccacatatg tgctattcga
    85201 aaagttaagc tacaaggatt acccctttgg cattactgct ggtgtagcct acattgctgc
    85261 aagttttaag tatgtttttg tatcgcttgc cgataatcct gtaattcaga aaaattggac
    85321 cagtccagca gcttttgttg agtttataat gctgtttggg ataattctgg gcgcatacat
    85381 tggtgcaaag cttagtaaaa actactcccc agaggtaatt cctgctgttt gggagaaata
    85441 ccatggcaca aacgtatgga aacgttttgg ggtagtgttg cttggaggtt tctgcttagg
    85501 attagggacc atgctggctg ttggttgcac aacaggtaat atcctgcagg gctgggctca
    85561 cctatcgctt ggtagtatgg tggctggagc ctgtttcttt attgccggaa ttattgttgc
    85621 aaagttactt taccctaaag ctggaggacg ttaatcatgt tactacaaat tttaattgga
    85681 ttggcaatag gcctaatatt cggctatgcg ctacaacgta ccggggttac aaagtaccca
    85741 agggtaacag ggatgcttct gctaaaggat ttcaaaatcc taaagttcat gctaacagca
    85801 gtaacctttt cgatgattgg tttttacatg cttggaaact tgggtgttat tgaactacat
    85861 cctaaagcgc tagattgggg caaagtggtt ggcggattaa tttttggaac agggatgggt
    85921 cttcttggtt actgtccagg aacgctgttt gcccgtattg gcgaagccaa aaaagacgca
    85981 tgggtagcac tgcttggaat gcttctgggg attctattct ttgcagtgaa tgtgaagttt
    86041 ttcaagtcaa acctgatgac caaaaacatt aatggcgata tatcggcagt gctgggcata
    86101 aaccaatgga taattgttcc tatagccgca ttggtttttg ttgccattat ctaccttgcc
    86161 aacaaggttg acgattttaa gaaaaacttt taagtggtaa gccattaatc caaaatgttt
    86221 acaccgtgta tatgttgcct ccagggaagt tcccgacgag gaaattgtgc gacaggccat
    86281 tgttaaggaa ttaaacaacc cttaggatac acctattctt agagaaaaaa tttatgctta
    86341 acactaaaaa aaacagttca atgaaacgac tagtaattta tttaaccatt accgcatttt
    86401 ttatagcggc tctttcatca aatacgtttg cgcaaaccaa aagccaaacc agtaaaacag
    86461 aaaggaaata caaggtaacc tttattgagt tgggttcagt tagatgcatc ccttgccaac
    86521 aaatgcagcc tatactaaaa tcggtagaag aaaagtatgg caaacaggta aaggtaatct
    86581 tttacgatgt ttggactgac gatggaaagc aatacgccaa aaagtataat atcaacctta
    86641 tacccaccca aattttttta gatgagaatg gtaaggaata ctttaggcac gaaggttttt
    86701 tccccgaaaa cgaacttatt gaagtgctca agaaaaaagg ggtaaaataa aatcctccat
    86761 tctttttaac tcattttaac atcgattctt gtggtattta tggaaggaat tgtatgtttg
    86821 caagtgttta cttacttgat atatgaggca acccactgag gttcggcagg aacagattaa
    86881 gcaggcagtt ctcggtatta tttataccga tggattgaaa aacctatcca ctcgtatgct
    86941 ggctcagcgt attggcatga gtgaaggtgc aatttttagg catttcccaa caaagcaaag
    87001 catcattcat tccattataa ctgacgtaca aagagatttt attgaacact tacgaaagat
    87061 cgccacttct aacgaactgc cacaaaaacg cttggaacac ttactttgct ttaccgttaa
    87121 gtacctaacc gataacaagg gtattaccct actactcttt tcagaagcat cacataataa
    87181 cgatgaagag ctaaaacagg ccttgatgca gatatttaat acacaaaaaa aaatggtaag
    87241 caaggttgtt cttgacggta ttgctgaagg gatatgggac gaaaacatac ccgttgaaaa
    87301 cgtagccatg ctatacatgg gcatacctgt ttcacttaac gttgaactaa tactcaacaa
    87361 gggtgtttta gacacgtcga acttttgtag taggatgatg tttctcataa acaaaatgct
    87421 caccaggtaa tttttttaca catcatgtaa gtatatactc actttaattt taaaaatatg
    87481 cttttcataa ttgtatttat agtaatttca atagcatcgt tcattttttc catgctagga
    87541 ttaggcggag gaatggttta cgtaccagta ctaaactggg ctgggtttga tatggttacg
    87601 gttgcaattc ctttgggttt gcttttgaat ggtttaaaca cagcattggt actcatcccg
    87661 tttgcccgta aaaagatggt tgattggaag ggtggtgccg taatggctgc tactgcatta
    87721 gtagcttccc cgctaggcgc aatgaccagc gaacatgttc cagttcaatt gcttaaattc
    87781 ttatttgctg taatggttgt tgctgctgcc ctccgcacgc tttgggcgtc aaaacaggag
    87841 gagcccgaaa ccatgatgtc gttaaagaaa cgaagtatca taggtttctt tgtgggtggc
    87901 tttgccggtt ttatcggtgg catgctgggt ttaggcggcg gctttatcat tgcaccaatt
    87961 ctcatgatga tgggctacaa aacaaaggag gctgcagcca caaccgcatt tgtggtgaca
    88021 ttctcatcct tttcgggtta cctggggcac gtatcccagg gccatataaa ttggccactc
    88081 accatagtag ttgtccttgc ggtaatcata gggtcacaac tgggcggttt atacatgacc
    88141 caaaaagcta aatcgaaaca ggtaaaagtt gtatatgctg ttgttcttct actcatagca
    88201 actaagatgg tttacggaat tttatcttaa aaattgtacc catgagccat gatcataacc
    88261 accataaggt aagcgggaaa aacttaggca ttacaatact tctaaacctt tttattacag
    88321 ttgccgaatt ccttggcggc atattttcag gaagcatatc gcttcttagc gatgcagccc
    88381 ataacttcag cgatgtgcta tcgctaatta taagttacac agcaaatagg ttatcaggac
    88441 aaaaagccac tcttactcgt acctacgggt taaaacgctc cgaaatcctg gcagccctga
    88501 taaactctgt tacactacta ggtttagcta ttgctattct tttggagagc gtcaaacgcc
    88561 tttataaacc cattccaatt gattccggtt gggtgatatg gctatcgtta ttaagtattt
    88621 tggttaatgg cttaagcgtt cttttcataa aagctgattc acgcaatagc atcaatatac
    88681 ggtcggcata ccttcatctt ttcagcgata tgcttacctc tgtagccgtt ctgattggtg
    88741 gactggctat gaaatacctc caatggtatc gggttgatgc cctctttagc atactaattg
    88801 ccatatactt actttttaca tcgtggaaaa taataaaagc ttccttgaga atttttatgc
    88861 aattttcccc tgagaatata aacatcaatg aaattactaa acaggttgaa tcacttgagg
    88921 gggtaaaaaa cttacaccat gtgcacatct ggcaaattga tgagcacgaa atcatgttcg
    88981 aggcccattt ggatgtaact aacaatatca caatatcgga tttcgagaag attcttgagc
    89041 aagtaaaaat tttgcttgca aagcaaggca taaatcatgt aactattcaa ccggagtatt
    89101 cggttactga taataaacaa ctaattcatt aacaattaaa tttttacact atgattaatc
    89161 ctaaagattg gttggaattt ggtcaaaaat tccatggaca taaatgtcct gcaatgccta
    89221 tggggcttag ggtaggtgct gctgcaatga atgcactggg tgtggaacgt gctaaggatg
    89281 gccagctaat agcccttgtt gaccttggcg atgaccattg tgccacctgc tatgccgatg
    89341 gtttacaggt tatactgggc gcaacctttg gcaaaggcaa tatcaagaaa acccataaag
    89401 ggaaatgggc cgttactgtt attgacaaag ctaccggccg cgctgtaagg gttacaccca
    89461 aagcagaagc aatgcttgcc aataagcaaa catcattctt taaggattac cgcgaaaagg
    89521 gaatacccgc aagtaaagtt cccgatttgg tggttgaccc tttggttaat aatgtaatga
    89581 acgcccctga tgaaaagtta atcaatattt cagaggtctt ccagtatgac cttaaagaac
    89641 acccacacag ttttaatagc tttgtatgcg aggagtgtgg cgaaatgacg gttatggagt
    89701 acggacgcat taaaggagat aaaaaggttt gcatcgattg tgctaacaag tactaaaact
    89761 acctataatg caaaaacggt tggctaaaac accagccgtt tattatttca ttccaactaa
    89821 ataaacatta acaggctgat tgacatgaca atcatgccac caataaggcc gtaaatggaa
    89881 aggtggtgct cgccatactc gcgggcagca gggagtaact catcaacaga gataaatacc
    89941 attatacctg caactgctgc aaacagaata ccaaatgtaa caccattaat aaacggcata
    90001 agcagcaaat atccaactat agcaccaata ggttcagcaa ggcccgagag gaacgatagc
    90061 ctaaacgcct ttcgtttatc gcctgtagca tagaaaattg gtaccgaaac agcaatacct
    90121 tcaggaatat tgtgtattgc aatggctaca gcaatggcaa tccccaggtt agggttcgat
    90181 aaagccgcgg taaacgtggc caagccctcg gggaagttat ggatggcgat ggctaaggct
    90241 gtaaagagtc ccatgcgcat cagtttccct ttacgctttc ggccactctc agcatcctca
    90301 accagcataa cctcgtgggg gttttcctgc tcgggtataa acttatcaat cagcgcaata
    90361 accgccatgc ctccaaaaaa ggaggcaacc gtaatccatg aacctaaatg caagccaatt
    90421 gcatcgacca gcgactcacg cgccttggaa aaaatttcaa taaacgacac ataaatcatt
    90481 acgccagccg aaaaccctag cgcaagcgac agaaacttag tattggtatg ctttgaaaaa
    90541 aaagcaatag cacttccaat accggttgaa agaccagcaa aaagtgttaa accaaatgct
    90601 ataagtatat ttgacatctc atactccata tctcacggta ttaaatgaac cttagtaaac
    90661 aaagttaacc aaaataagtt tagtaggccc ctatcctaac ttccaactag ttcattatag
    90721 cttttcacct ccatttggat attatcggta ttcagaaacg cctcaataaa agcggctgca
    90781 agtcgagcat tggtaattag cgggatgttg taatcaacag ctgaacggcg aatggtataa
    90841 tcgttatcga gctcgctgcg cgagagattt ttagggatgt tgattaccaa atcaaccagt
    90901 cgattctgaa taagattcag gctattggga gcattttctg gctcatcggg ccaggaaaca
    90961 gcaatggcag gtatgccatg gtcgttaagg aacgatgcag ttccccttgt tgcatagatt
    91021 acaaaaccgc gcctggccaa acgggcaatg ggatcgataa gggagagttt tgaccttaaa
    91081 ggcccagatg aaaccaagat tgaaccctgt ggaattctat ggccaaccga caacatcgct
    91141 ttaagtaagg ccacattaac actttgccca atgcagccaa cctcaccggt tgaggccatc
    91201 tcaacaccca gcactgggtc ggcaaaggta atccttccaa atgagaactg tgaggctttt
    91261 acacccacat gggcgatatc gagcatcgat ggtggattgg ttttaggtgc tacacccatc
    91321 ataaaatcaa cggcaacggc tatcatatca aaattcatca cctttgaaac aaacggaaaa
    91381 cttctacttg ccctgaggtt gcactctatt accttaattt ggttgttctt tgccaaaaac
    91441 tgaatgttaa acgggcctgt aatttttagt tcggctgcaa gagctgcagc aacacgccta
    91501 atacggcgga tggtttccag gtagaggcgc tggggcggaa ataccagggt agcatcgccc
    91561 gagtgaacac cggcaaactc aatatgttcc gatatggcat caacaaatat ccgaccatta
    91621 ttagccacag catcgtattc aatttccttt gccccttcga taaactcaga gataactact
    91681 gggtgggctt tggacacttc ggctgcaaca gttaagtact cgttcaaatc gtcggcgttt
    91741 gaaaccacat tcattgccga acccgaaagg acgtaggaag gacgaattaa aacgggataa
    91801 ccaacacgat ttacgaactc ctgcacctgt tccatggtgg ttaactcctg ccactctggc
    91861 tgatcgatac cgagtttatc gagcaaagcc gagaattttt gtcgatcttc ggccatatca
    91921 attgaaacgg gtgaggttcc cagaaccatc acccctgctt tactcagcct tcctgccaga
    91981 ttattgggta tttgtccacc aaccgatact attacaccta acggacgctc aacctcaacg
    92041 atatccatca ccctctcaaa ggaaagctca tcaaagtaaa gcttatcgca tacgtcgtaa
    92101 tcggtactta cggtttcggg gttgtagtta actacaatcg cctcaaatcc caagcgtcgg
    92161 agctccttta ctgcactaac ggtgcaccaa tcaaattcaa cagaactacc aatacgatat
    92221 gcccctgaac ccaaaacaag aactttgggc ttaggtttta cggatgtatc gtcctcattc
    92281 ccatggtagg tcatgtaaag gtatgcggtt tccgatggga attcggctgc caatgtgtca
    92341 atctgcttta cacaaggaac aatcccacag cttttcctgt agttccgtac ctgctgttgg
    92401 tactgctcaa ttttattgtt ggatggattg taaatgatac gagccaactg gaaatcggaa
    92461 aagccattcc gtttagcttg aacaacaagt tccgatggta cttgctcaat ggctgagtaa
    92521 cctgcaagat gcctggaaag ctcaactata ccctgaagct tatcgagaaa ccaactatca
    92581 attcttgtga ttgcattaat atcctccgca gtccatcctt gctcaaatgc ctgggcaatg
    92641 gcaaaaattc tttgatcagt aggattctca agcatcagtt taaggtccga cgggttaaaa
    92701 tgctgatttg ctacaagccc atgcattccc tgccctatca tcctaaggcc tttttgaatg
    92761 gcttcctcaa atgaacggcc tatggccata acctcaccca cgctcttcat agccgaccca
    92821 atttggcgcg atgccccctt gaacttagct aaatcccatc ggggtatttt taccacaaca
    92881 tagtcgaggg caggctcaaa gaatgccgtt gttttgtggg taacggagtt ttttagctca
    92941 aacaggccat aacctaaacc taacttagca gctacaaatg ctagtgggta accggtagct
    93001 ttggatgcca aagccgatga gcgcgataag cgagcgttta cctcaatcac tctataattc
    93061 tcactactag gatcgagtgc aaactgaaca ttacactccc ctactattcc caggtgacga
    93121 actattttta ttgagagcgc ccgtaattta tggtactcgg agtttgatag tgtttgcgaa
    93181 ggtgcaacta ctatgctttc gccagtatga atgcctaatg gatcgaagtt ttccatattg
    93241 caaactgtta tgcaattatc gaacctatcg cgtaccacct cgtactcaac ctctttccag
    93301 ccctttagcg attcttcaac cagtatttgt ggtgcataag tgagcgcatt ggttgccaaa
    93361 gcctttaact cttgtgggtt tgagcagaat ccgctaccct gccctcccag agtaaaggca
    93421 gcacgaacaa taaccgggta gccaatttga tgagcggcat caaccgcttc agccacagta
    93481 ttcacggcaa tgcttcgtgg agtgctcacc ccgatttggt ttaactcttt aacaaagagt
    93541 tcccgatcct cagtgttgat aatagtgcta actggcgtac caagcacttc aacattgaac
    93601 tcccggagtg tgccattctg gtaaagttcc accccgcagt ttagtgcggt ttgtccacca
    93661 aacgagagga atattccatc gggttgttcc ttctcaatga tttgtcgaac cgtttcgggt
    93721 tttacaggtt caaagtagat ggtatcggcc acatcctgac tggtttgaac cgtggcaatg
    93781 ttgggattta ccagaactgt tgctatcccc tcctccttta gtgccttaag cgcctgtgaa
    93841 ccgctgtagt cgaattcgcc ggcctcgcca atcttaagcg ctcccgaacc aagcactaaa
    93901 actttgctgt atttgcgtac aagtggcata taaatctatt ttacttgttg tcaattaggt
    93961 taataaactc atcgaacagg aactcggtat cgtttggtcc tcccgaagcc tccggatgaa
    94021 actgaactgc aaaaaaaggc ttggctttat gccttatacc ctcgcaggtt ccatcgttca
    94081 ggttttcaaa catcacctcc cattcatcgg aaagggtcga tggctcaatt gcaaaaccat
    94141 ggttttgcga ggtaacaaag cagcggtcag ttccttttag cctcacaggc tgattatgcc
    94201 ccctatggcc atagggaagc ttataggtat ttgcccctga ggccaaagcc aaaagctgat
    94261 tacccaggca aattcccatt accggtttat ccatttcaat tagtcgacga atattctcaa
    94321 tggtttcaat gttcaccttg gggtcgcccg gtccattgga gatgagaaca ccatcgaatt
    94381 gctggctcaa aaagttatag ttgtaaggca cgcgaataac ctgtacatcg cgtcttaaaa
    94441 ggcaacgcag gatgttgttt tttactccgc aatcaaccag aacaattgtt ttctttccat
    94501 tcccataggt aattggtttt gccaccgata catgtgcaac cgggttatcg gcattgggat
    94561 cgtaccaatc cgtatcgcca tcaactataa tttttcccaa catacaaccc ttttcgcgca
    94621 attgtttggt aagcttgcgg gtatcaatac cgcttattgc cggaatcttg ttttcattta
    94681 gccattgccc taggcttttg ttcgcattcc agtgactata attttggctg tactcgcaca
    94741 ctactaagcc cttaatgtga atcctgcccg attcccaatt ctgttcaatt cctcccaaag
    94801 gattaggttc gggtacccca tagttgccaa ccaaggggaa agtggccaca agaatttgtc
    94861 cgaagtacga cggatcagtt aaactctcgg gatatccatt cattgccgta ttaaaaacaa
    94921 cctcacccga tgtgttaccg gcatacccaa acgagtaacc agtataaact gagccatcct
    94981 ccagcacaag ctttgctttt tgttgttgat tcattctgga ataatttaag agatttatag
    95041 tttgaagttc gataaatcaa ctttaaactg aacgtttgca acccttttct gtagggcaaa
    95101 aaaaaacctg ccaaaactgg caggttatca atggagttag ctgaaagtat tgtttgttcg
    95161 aaacctgtat gctttactga cagcaatatt tttttaagtg gaatcatatc ctgaatgttc
    95221 atacaaaact acaacttttt tctaaaaatc aaaactttac aaaaaagaac agaaagagtt
    95281 tgcatattac aacttttgat ttttaaattt gagattcaat ttgaataaaa tggagaacga
    95341 agttaagcag gcgtacctta agtattttga agattatgtt caacgcaagc tcgaagatac
    95401 gcttaatgcg ttttctcctg attttaacat gattggaact ggagtagatg agttcagcgt
    95461 aacatatcag cactccactg aactattccg acgggaattc gcccaggcgc ccgaaccttt
    95521 tacctatcag tttaaaactt taaaggtatg tcccattaca gccgattcgg cctatgtaat
    95581 gggtttagtt gacatgaagt tcctgctccc aaccggcgag tttgaataca caaacaatag
    95641 aacaactgcc attctacgta aagaaaaggg tgagtggaaa attattcacg gtcattggtc
    95701 gcagcccaca gagatgcaac aggagggcga aagtgtaccc ctatcggctg tactcaagca
    95761 aaacagggaa cttaagaatc agcttattat caaacaacag gagctggaaa agcagaacca
    95821 gcagctaaag gaaatgaacc aatcgctttc aaaacttttc tctattgtgt cgcacgacct
    95881 taaaagtcca ttcaacgcct ttctgggaat aactgacctt atgctgatga actttgagga
    95941 cgattttgaa aacaaggagt attttcagct tcgccttcag ctgctaaacg accttgccca
    96001 caacctttat gatttaaccg agaatcttct aaactgggca aagctaaact ccaatgccat
    96061 tgagcccaaa tacagggaaa tcccaattga ctccctgata caaaagcagg taaacgttct
    96121 gcgccccatt tggcaaaaaa aacgaatcac gctaacagaa gaagttccca gtgaacttat
    96181 ggtaaacacc gaccccgata tgctggctgt catcattcgt aatctggttt caaacgctat
    96241 aaagttcact tacccggaag gtgaaataaa aatccaggtt agcaatgagc ccaccatcct
    96301 ttccatcaaa attgttgata atggtattgg tatgacagcc gaacagctta aaaatctttt
    96361 taagaattat caatcaagca gaggaaccga taacgaaaaa ggcactggaa taggtctgtt
    96421 tacctgcaaa gagttaacca acaagctaaa aggcaacctt aaatttatct ccgaaccaaa
    96481 caaagggaca acagtaattg ttgaattacc caaataacca tttccagcaa tggcaccgat
    96541 tccaaaaatt gttcggtgga ttgaactggc tgcccttttt gttgggctac ctgtagcttt
    96601 ctacttcaac aacctgaaat tacccaaaag cattccgctt ttagtagtgt ttttaattgc
    96661 actattttat ttactcacca ccaaatcgtt tagtaaaaaa tcgtttggat ttaatggata
    96721 cagaagttgg agaacgctta gcttgcgtat tctcatttca ctactggcaa taagtttact
    96781 tagttttatc ttccttccta gtgagcaact cttttactta ccattacata ggacagagct
    96841 ttggctacta atcatgtttt tttaccccat ttggtcagcc tttacgcagg aagttatata
    96901 ccgtgggttt ttcttccacc gatacacacc ccttttcaaa aatgataaaa ttgttatcac
    96961 attaaatggg attctttttg gattacttca tataattttt agaaactgga tagctgttgc
    97021 tggtgctacc ataattggtt ttgtttgggc atacagctac tttaaacatc gctcaatact
    97081 agtagtatca ttagaacatg caattgttgg caatttccta ttcaccattg gattaggcta
    97141 cttcttttat gtacccgatt tttagtgcta ttatagaact agccatattt aaaaccatca
    97201 attcaagcct gcactgaata gtttttaccc caatgagcgg attttcccgt ttgccggtaa
    97261 ctgaatatct ttataatgac attgaaaaag ataaagtatt tgctcaggat taaaaagagc
    97321 atttatcgca tagtttacaa aaggatatga actttttttg ttgaggttaa ctaactttca
    97381 ttttttaaaa ccaaacacct tgcagcttgt taataaataa atgcctaagg catttccatt
    97441 taacaaacaa aaaattaact ttacaaacag attactatgg cgacaacaat cttaaaacca
    97501 ggcgatttgg ctcctgaatt cattgggaag gatcaggatg gtaaaaccat taaactatcg
    97561 gattttaagg ggaaaaagtt gatcctttac ttttacccca aggacaacac cagcggttgt
    97621 accgctgaag cttgcagctt acgcgatggt tatgatgatt tacagagctt aggcatggaa
    97681 gtggttggcg ttagccccga ttccgagaaa tcgcatcaga attttattca taaatacaac
    97741 ctgcctttcc gcttaatagc cgacgtagac catgcgattg ccgaaaagta tagagcatgg
    97801 ggtgagaaaa agatgtacgg taaagcatac tttggtattc tccgcacaac atatgtgata
    97861 aatgaaaatg gcactatcac aaatgttttt aataaggttg ataccaagga acacgtaaaa
    97921 caaatactat ccgaaataaa taagtaaatg gaagcaactg aaaacaagga acgcaaggaa
    97981 attaacaagg aaaagctaaa ggcccttcaa tcgacaatcg ataaaattga gaaggatttt
    98041 ggtaagggtt caataatgag gcttggcgat aaagccgtat cggaagtgcc agtcatctca
    98101 tcagggtcaa tagcgctcga tgtagccctt ggggttggtg gatatccccg tgggcgagtg
    98161 gttgaaatct acggccccga atcgtcgggt aagaccactc ttgccatcca cgccattgcc
    98221 gaagctcaaa agcaaggcgg ccttgccgcc atcatcgatg cggaacatgc ttttgaccgc
    98281 acctatgccg agaagcttgg cgttgatgtg gagaatctac tttttgccca gcccgataac
    98341 ggtgagcaag cccttgaaat tgccgataac cttatccgct caggctcaat tgacatcatt
    98401 gtcatcgact cggtggctgc ccttacccct aaagctgaaa ttgaaggcga tatgggcgat
    98461 agcaagatgg gccttcaagc ccgactgatg tcgcaagcgt tgcgtaagct cactgccacc
    98521 atcagcaaaa ccaacacctg ctgtattttc atcaaccagc tacgcgacaa aattggcgta
    98581 atgtttggca accctgaaac caccaccggt gggaatgccc tcaagttcta cgcttcggtt
    98641 cgtgttgata tccgtaagct gaaccagctt aaggatggag aggaatcgac cggtaaccgc
    98701 gttagggtta aaattgttaa gaacaaggtt gcgcccccat tccgcaaagc cgagttcgat
    98761 attatttttg gtgagggaat atccaaaaca ggtgaaatca ttgaccttgg cgttgaactg
    98821 aatatcatca agaaaagcgg ttcatggttc agctatggcg aaactaaact tggtcaaggc
    98881 cgcgatgctg ttcgccagct cctgctcgat aatccagaac tatcaaacga gattgaaaca
    98941 aaaatcaggg aaacgctcaa agcgcagtaa aacaacacat taaccacaca atactgcctc
    99001 aaaaagataa agggcaacgg ttttgaacct atctcagcat tgcgttcaat acatggcctg
    99061 aacaaaaaaa tctttttgag gcagttttct tttattttac cataaaatca caatttaccc
    99121 aacaccttta ggtttgaatc gaaaaattac ttacatttat tttttgatta caaacagctt
    99181 acaacatgaa gaagattgta actatcagcg cattgctgct tataatcctt agctcctgcg
    99241 ataccattct taaccgccac ctcatcaaag gcagcgataa acgcaaagag gttcatgccc
    99301 aattcgagga aagggtcaag ctaattgctg cctacgattc atccatttac aaaataatca
    99361 actcagaact taccactaaa gagcgcgaag ggtttgagtt tctttacgca tacatgccac
    99421 taagcgattt agcaatgcac tcaccagagt atgttctgaa gaacgttcag ctagcgctaa
    99481 aagcgcggaa agaatttaag tgggcaagga aagtgccagc tgatatattt cttcactttg
    99541 tcctacctta cagggttaac aacgagtaca ccgataacgc ccgcgagatt ttcttcgagg
    99601 aactaaagga gcgccttctg ggcctcaata cataccaggc tgcgcttgag gttaattatt
    99661 ggtgccacga gaaggttacc taccaatcga ccgatgagcg cacatgtggg ccgctaacat
    99721 cagttcgttc agccattggc cgttgtggcg aggaatcgac atttgcagta gcagcctatc
    99781 gttcagtagg cataccagca cgtcaggtct acacaccccg ctgggctcac accgacgaca
    99841 atcacgcctg ggtagaggta tgggtcgatg gtaaatggca cttcttaggc gcatgcgaac
    99901 ctgagcctga actcgaccgt ggatggtttg caggacctgc caaacgagcc atgatgacac
    99961 gtacttttgt ttttggcaaa tacaatggcc ctgaggaaca acttaaacaa accaactatt
   100021 acactgagat taaccttatg cctaactatg cccctacccg taaactcacc gttaccgtaa
   100081 ccgatagcac cggaaacccg gttgagggcg caaaggttga ataccagctt tacaactacg
   100141 ccgagtttta ccccatagcc accctgcaaa ccaatgcaaa tggcttgtgt actttaacca
   100201 cagggattgg cgacttgatg gtttgggcac aaaaggataa cacctatgcc ttttccaagg
   100261 ccgacagaaa ccaaaacgat atttacctaa cactcaaaaa ggaaatccct tttactaacc
   100321 tttccttaac ccttacccca cccgacgaac aaactattcc accatacgat gaaagtacag
   100381 ccgatgaaca caaccgcagg gttaaacagg ggaatgaaat tcgtagtaat ttcataagca
   100441 cctttattga ctcggcttca gcagccaatt tagccatgga aaagggtatc agcattgatg
   100501 aaacatggaa atggctttca aaaagccgcg gcaactggaa tgagatttac agctacataa
   100561 agaacttgga cactaaagac atctctgttg gagtggcctt gctttcaact cttcgggaaa
   100621 aggatttacg cgatattacc accgatgtac ttttcgacca cctttcacga atcgattctt
   100681 tccctgccct gcttgaaagc cgcatggtta aagagtttga cagctacatt ctttcgcccc
   100741 ggattggccg agaatttatt acaccctggc gcagtttctt gcaggatgcc tttacctttg
   100801 aggagattgg ttttttccga aacaatcctc aaaacattgc ccaatggatt aaagagtttg
   100861 taacacttga caccgaaagc aattactacc gtgtgccgtt aagccctgaa agcgtttacc
   100921 ggattcgccg cgccgatgaa tattccatgg ctattttctt tgtagcagca tgtcgcagtt
   100981 ttggaatagc tgcccgattg gagccagcat caaaaaaacc tcagtacttc atgaaaggga
   101041 agtggattga tgtaaatatc gccgatgtaa aagagacaca ggccacaaca ccaaccggga
   101101 tgctaattat caacctcgat aaaaactcgg ctgttgaaca gccaaagtac tacacacact
   101161 tcaccattgc acggttcgaa aagggaaagt actcaaccct tgattatgag gaaagtcctt
   101221 tattcaacaa attccctgca gcacttgaac tcccagaggg ttattaccgg atagtaaccg
   101281 gtaacaggca taaatcggga gcagttaact gcaatgttta ctacgtcagg gttgaaagtg
   101341 gcaaaaagat acaatttaca ctaaccatcc catcaaccca aaccgagaaa atggttttgg
   101401 gaaccattag caccgatatc accatacctt ttatccagaa cccccaaaca attgaacaga
   101461 ttaccaagga taaaccagca atagttgtca ttatcgaccc gcttgctgaa cctacaaagc
   101521 atttgcttcg cgatatcagc agcctatgta gcgagtttag tggcatagga aatagtattg
   101581 cccttattct ggcaagtggc aaatcggcaa aaaccgattt gacatccatt ttcgatggaa
   101641 agccctaccc caataccatt accctcggga acgatataaa tggccaattt gaggctaagg
   101701 tagggcaggc agttggcaaa cagctggaat accctgttgt tcttgttgtg aacaacaacg
   101761 gagagatagt atatcattct tcgggttata gcataaacct tggcgaacaa ctactttcag
   101821 cactaaagca gtaaacgatg tttacccttg aggtttgcgc caactctgta gagagtgcca
   101881 ttaacgctca aaaaggcgga gcaaatcgaa tagaactctg tgagaacctt tacgttgggg
   101941 gtaccacccc atcgtttgga tgcattgccg aaacccttaa ccagctaaat attccggttc
   102001 acgttcttat tcgtccccgc cccggcgact ttgtgtactc cgaaatggaa ttccgtcaaa
   102061 tgctccgcga tattgaggca tgcaaaaaac ttggggtaaa aggggttgtt agcggtatac
   102121 tccgggcgaa tggtactctt gataaggaaa gaactaaaga actggtttgg gcagccaaac
   102181 caatgacctt tacctttcac cgcgccttcg acataatgcc cgaacctttc aaagcactaa
   102241 atgaaattat cgaatgtgga tgcaatttcc tactaacgtc agggcaaaaa aacaaggctg
   102301 ttgatggttt agacctgata aggcagttaa ttgccgaggg aggtaacaaa atagccctaa
   102361 ttgccggagg agggattaat cacagcaata tcttagagct agcacagaat ggtgtaaacc
   102421 agttccatat gagcggcagc gaaattgtag cccggtacaa taccaaccat accggacttt
   102481 catttgtttc ggcattgctt ccggaaaata ccgttcagca aaccaatgcc gaaaccattg
   102541 ccagcactgt ggcaaaacta aaacaatact tcaacaaaaa gtaaatacta accaatgact
   102601 agagaagaac ttgtaaagca tgcaaaacta ttccagcgag aggaaattac aggctaccag
   102661 gtttataaga atctggctaa atcaacaaac gacaaaaaca ataaaactgt tttagcaaaa
   102721 attgctggtg aggaacttaa gcactacaat attttcaaac agatatcggg ggttgatgtt
   102781 gcccctcaaa aaactgtagt atggtttttc accatttgcg ggaaaatact tgggcttaca
   102841 ttcgccttaa ggcttatgga gcgacaggag gcaaaaattc aggatgttta cgaacgagta
   102901 ataccctaca tgcccgaagc cgaagccgta aaaaaatccg aagaggaaca tgaagaccag
   102961 ctaattgcat taattcagga agagagactt gattatgctg gctcaatggt tcttgggcta
   103021 aacgatgcgc tggttgaact aaccggcgct ttagctggct ttagccttgc catgcaaaac
   103081 acccgactca ttgcagttgc cggacttatt acgggtattg ccgcttcgct ctcaatggca
   103141 gcatcgcagt acctttccgt taaaactgaa aatgatgacc gacacgcggg taaatcggca
   103201 atttatactg gtttggctta tatatttacc gttgcaatac tgattagccc ttttctactg
   103261 ctcacaagct acatgctagc cctagcaaca acacttctgc ttgcaatagc catcattttc
   103321 gccttcaact tctacattgc agtagctaaa aacctcaact ttaagcatag gttccttgaa
   103381 atggttgtta ttagcttagg agtttcagcg ctaacctttg gtataggctt gctggttcga
   103441 aactacctgg gaattcctat tgactgaatt aaacaaaaat tgatatggaa ccaaaaaatt
   103501 acaagttcat ttcgcctttc gagttaaatg aaaacacctt cgacctgctc gacaatcaat
   103561 ggatgctaat acttgctggc agcaaggata aacataacag catgacagcc tcgtggggag
   103621 gatttggaat actatggaat aaaccggtgg catttgtatt catacgccct cagcgctaca
   103681 cattgcagtt tgtggagcaa aacgattact ttaccctcaa cttttttgaa gagaaatggc
   103741 gttcggcact ggagctatgt ggcagcaaat cgggtcgcga tatcgataaa actaaggcag
   103801 cgggattaac cattgccgaa tcgcttaagg ccatagcctt tcaggaggcc cgactggtga
   103861 ttgaatgccg aaaactttat atcgatgata tcaaacccga gaactttctc gataaggaaa
   103921 tcatcagtaa gcactaccct aaaagcgatt tccataggct atttatcgct gaaatcacat
   103981 cggtattcaa aaaagagggt tgatggctta aaggaaatgt aacaatacag cagcgagtaa
   104041 tagcttgaat caaaagactc cttactcatt ggattcatcg tcggcatcag gctcaaagct
   104101 atcccaatca aagcccccga aaccggaatc gaatagttca tcaatctcgc gacgttcctt
   104161 ttttgtgggg cggcctgttc cgcgttcgcg gtgtatgttg aatgccatga acgatgggtc
   104221 aagtttctgt aactcctcgg gaggagttaa gttttccata tacgattcaa ccaacttggc
   104281 gccaagccgg tttttgggta ggtctttcac cctgaatgta taggtcaccg ggggttttcg
   104341 aacctttaat gtatcaccta cattaatatc ctgcgagggt tttacctcaa tcccattacg
   104401 ggttacccga cctttgcggc aatactcggt agcaagggtt cttgttttaa ataccctcac
   104461 aaaccacaac cacttatcaa ccctcatggt cggcattcta tcggctattg taaaggttca
   104521 tggagcgttc aataccaatt gtgccaaagg caagcacagc ctcgccggcc aacgttagcc
   104581 gttcgggcag taccttcagc tcctcatcgg tccactcacc caaaacgtaa tcgacctggc
   104641 ggccacgact aaatccatta ccaattccaa aacgaagacg ggcataatcc tgtgtgccca
   104701 gcacgtcgtt tatattcctc aacccgttat ggccaccgtc gctaccttta ggtcttaacc
   104761 ttattgcgcc aaggggtaag gcaatatcgt caactaccac caagaggttt tccaatggga
   104821 tattttcctt ttgcaaccag tagtttaccg ctttaccgct aaggttcatg taggttgagg
   104881 gtttaaggag tacaaatgtc cgccctttgt gcttgagctg agcaacaaag ccgtagcggg
   104941 catcggcaaa agtaatgttg gacgccttag caagagcgtc caacaccata aatcctacat
   105001 tatgtcgggt gttggaatac tcacttccaa tattccctaa cccggtaatt aaatacttca
   105061 attctgaact acttatttag cttgttcctg ctgagcttcg cgagcagcac gggtcgattt
   105121 aatggtagca attaccatgc tcttaggctc aaccacagta aacttatcaa aacttagttc
   105181 agctaccata atgctcttgc caattccaag atgggtaaca tcaatggaga tggcatcggg
   105241 caggtcctta gctaaacctt ttaccctaat tttgcgcact acctgcaaaa gttttccacc
   105301 agccttaaca ccctcagcct gtccaaccag gttaactggt aaaccaatgg ttataggttt
   105361 gttctccgaa accttaagga agtcaacgtg tagaatctta tcggtaacag gatggtactg
   105421 aacctcttta ataatagcat cgtgcatttt accatcaacg ttaagcttta caaggtaaac
   105481 aataggtgtg taaatcacct tcttgagttc cttctcgcct aacgaaatca taatgttctc
   105541 accagcaccg taaataacgg cgggtacaag ctccgacttg cggctgtgct tagcagcttt
   105601 tttaccggtt tcggttctag gggctgctga aagttcaatt gttttcattt actcttgata
   105661 atttttgtta ttacttaggt actactcagg gctgtccctc aattgcataa ccagcccccc
   105721 atcgtaccct atcatgaggg aaatgggctg caaagatagt aaaaaaaata tttaaaacta
   105781 aatgatgaac ttttcgctga ttgactggta gttgtaaacc ttattgataa cgtcggcaaa
   105841 aagctcagca accgaaagca ccttaatttt ggaagtatcc ttatcggtac gcaggggtat
   105901 ggtatcggta acaaccagtt cggtaagctt tgagtcgcga atgcgctcat aggcagagcc
   105961 tgaaagaacg ggatgcgtag caaaagctcg taccgatgcc gcccctttac ccatcatcaa
   106021 atcggcagcc atggtaaggg taccggcggt atccaccata tcgtccagaa taattacatg
   106081 cttaccctca atatcgccaa taacagtcat ctcacctacc tggtttgctt tctcgcggtt
   106141 cttgtggcaa acagccagcg atgagtttag gaatcgagag tatgcgttgg ctcgctttgc
   106201 tccacccata tcgggagctg caatcgcaat gttttccaaa ttaagactct tgatataggg
   106261 gacaaatatt gatgacgcgt aaagatgatc aaccggcaca tcgaaaaagc cctgaatctg
   106321 gtcggcatgt aggtccatgg tcattacacg atcggcacct gctgcaacga gcatattggc
   106381 aaccaacttt gcgccaatgg caacacgggg tttatcctta cgatcctgcc ttgcccaacc
   106441 aaagtaaggc atcacagcca ccaccttata cgctgaggct ctatgagcag catcaatcat
   106501 cagtagcaac tcaaaaagat tatcaaccgg tggaaaggtt gactgaatga taaaaacagt
   106561 acatccccta accgactcat cgaatgaggg ttgaaactca ccatcgctaa aaacggttag
   106621 ggttgatttt ccgagtggaa ttccaaaact ttgagctatt ctttcggcaa gataacgcga
   106681 gctacgtccg gtgaagaact ttatttggtg tttgctgtgc attttaggat ttttttccgt
   106741 taatgctcgc aaatttacaa attttatagc tcatgttcta tacctggcaa tgagttattt
   106801 ttgcttcccg aaaaacctgg tcaatgtaat taagcacatc ctcacgtcct gtgcgagtta
   106861 cacacgatgt tgtaaaaatt ggaggtagtt cctcccacga ttcatgtagc acttttttgt
   106921 gcagctcaat gttctgctca agcaccatgc tggaaacctt atcggttttg gtgaaaacaa
   106981 gggcaaatgg aacctggtta actccaagcc agttaataaa ctggatgtcg atatcctgag
   107041 gttcaaggcg ggaatcgata agaacaaaaa gcaagtgcat attctcccgc ttgagtatgt
   107101 agttgagcac tatatccgag aacttttcgc gctcaccctt cgatacttta gcatagccat
   107161 aaccaggcaa atcgaccagg tgccatgagt cgtttatgag gtaatagttg atgagctgtg
   107221 ttttgcccgg tgtttgcgaa actttagcca aggccttggt gcaggtgagc atgttaatga
   107281 gcgacgattt acccacattg gaacgaccga taaatgcgta ctcaggcaaa tcggtttttg
   107341 ggcacttttg cacactagga taactagcca caaacttagc tgttcgaatc accatagctt
   107401 aaaattttag ttaaacaatt tgtcccagat cggtttcaaa gatacaaccc atcggaaaga
   107461 aaatcaactt tttgtgaata aaaaaaattg ttttaaagtt tatatggtaa atcatcaact
   107521 ttatttatca ttaacatatt ttaacattct gaatcatgtt ataaaacata ttaataaatt
   107581 tggcgcattg atgcgggggg ggggtaaatt tgcaacacaa tactacaata ataatgtaaa
   107641 tgatttgcat tatagtataa tgtttgccag atattgataa acaaaagaat aataaaagaa
   107701 catcacttca aatattgtca tgtaaatcta tctatttact agaaatcttt gatacgtatt
   107761 ctctttttaa gcaaaaacct tgataggaat ttaaaaaaca ttgaatatat gacagaatta
   107821 aaaagagttt ttcctggatt tgttatacaa aattctgtag agggttattt acattccatc
   107881 tcgaaaagaa gcaaaaaaat ctattggtta atcataatga taataatagg aactctagca
   107941 tctatgccat ttattttggt agacgttact attcgaacat caggattgat taagccaagc
   108001 attgaaaagc agaatattgt cttaccatat gggggtaagg tcatttacac taaactaaaa
   108061 aataatttaa gggtaagtgc aggcgataca atcctagtaa tagacaccat acccattagt
   108121 tctaaaatta aaactttaga gtttaatctt ggtgagttaa aatcatatca gaacgatttg
   108181 ggtttactta ttgagcataa ttttttacta caaaagttca atccaaagag tttggtaact
   108241 tccaaatata tgcttgaggt aagtagtttt cagcgtcaaa tatcaacaca aagtaatcgg
   108301 attgaaaagt tgaaaaaaga acatagccga aacacccttt tatacaagca aaaaataatt
   108361 gccgaggtcg attatgaaac aagtaagtac aacttggaca atgagatttt acttcgaaat
   108421 caatttataa gccagcagtt agccagctgg caagcaagcc tagccgagat taccaaccaa
   108481 attccaaaga ttgaatccga aatagaaaca gcatataatg aacttagcaa gcattttatt
   108541 acggcgactt tgagcggaac tattcaacaa tgtaatgagt tgcaagtagg tagtattatt
   108601 tatgccaatc aaataattgc agagttgtcg cccgattgcg agttaattgc agtttgctat
   108661 gtgcaaccca gcgacatcgg ttttatacgg gttaatcagg atgtgaaaat acaggtggat
   108721 gctttcaatt ataaccaatg gggtttgctg cctgcaacca taaaggaggt ttctgatgat
   108781 atagtaagtg atgggcagaa taatatgtac tttattgtta agtgcaaatt atcaaaaaac
   108841 tatttaacac ttcaaaacgg ttttaaagga tacataaaaa agggtatgac aatatcatca
   108901 agaataatag taacagagcg tagtgtatat gatttacttt ttgataaagt tgatagatgg
   108961 tttaatccat acactgaaat gaaatagcgg aaatgagagt aaaacaaaga gatattactg
   109021 attgtggtgc agcctgtttg gcttcagttg cagcgcatta taagttgaat ttacctatag
   109081 caaaaattcg tcagttcgct ggtaccgata gaaaaggcac caatgtttta ggaatgatag
   109141 aggcggctca aaaaatgggt ttccatgcaa aaggcgttag aggcgatttc gatagccttt
   109201 tcaagatacc aaaaccttca atcgcacatg tggttgttaa agaagttcta caacattttg
   109261 ttgtaataac taaaacaacc aaaaaataca ttgaagtaat ggatcccggc gatggtaggt
   109321 tccataaaat accgcacgaa gaattcaaaa agtcatggac aggtgtatta atccttcttg
   109381 tgccaggtga aacatttaaa cccaaatccg aaaaagtttc atcatctttt agattctgga
   109441 atcttataaa gccttacaag ggcgtattaa cccaagcaat tattggagca gttctatatg
   109501 cggttttggg cttatcaact tcaatttatg tggggaaaat agttgattat gctattccaa
   109561 gtaacaatgc aaatttactg aatcttttag ggattgcaat gctaataata attggggtaa
   109621 ggttcttgct aaacatattc cagtcggttt ttgttttaaa aattgggcag aggatagatg
   109681 ctacgctaat tctgggatac taccagcatt tgctaaggct gcctcagtcc tttttcgaca
   109741 caatgagagt tggcgaaata atatcaagaa ttaatgatgc agtgaaaatt cggttatttg
   109801 tgaacgaggt aaccgttaat ctaattgtaa acgttttaat tctaattgca tcgtttatgc
   109861 tgatgttcac attttactgg aagttggctg taattatgct tttggtaatg ccgctatata
   109921 tcattaccta tatgataatt aataggctaa accgtaaggt acaaaggagc attatggaaa
   109981 attcagcaga gttagaatcg cacttggttg aatccctaaa ttctatatca actataaaga
   110041 ggtttgggct tgaaggttac tccaacttaa aaaccgaaat gcgatttgtt aatttgctaa
   110101 aaccattata taaatccgga attaactcaa tatattccaa caatgtttca ggtttaatat
   110161 cacaggtaat taccattgtt ctgctttggg ttggtgctgg ttttgttcta gaaagcacaa
   110221 ttacccctgg cgaacttctt tcattttact ccttaatagg ctatcttact tctccagtaa
   110281 ccagtattat tggttttaat aaaacttacc aagatgctaa aattgcatcg gataggttgt
   110341 tcgaaatttt tgacttagaa gtggaggatg aaaaaaacaa aatagaattc accgctgagc
   110401 acgttggcga tattaccttt aaaaatgttg atttccgcta cggaacaagg gtggaggttt
   110461 ttaaggcgtt aaacctgcat ataccaaagg gaaaagttac cgctgtagtt ggcgagagtg
   110521 gttcagggaa aactacccta acatcgctgt tgcagaatat atatccatta caaagcggaa
   110581 atatttacat aggaaagttt gatttacggt actttaccaa cgaatcgtta cgcgatgcag
   110641 tggtggctgt accgcaaaat atcgacttat ttgcaggaac aatagttgag aatattgcag
   110701 tgggggagtt taacccaaac attcagagga ttattgagat ttgcacccag ctaggtatga
   110761 ttgagtttat agagagccta cccaataggt ttaataccta cattggcgag cacggtacat
   110821 cattgtcggg cgggcaaaag cagcgcattg ccattgcccg tgcactatat aaaaatcctg
   110881 agatactaat actcgacgaa gcaacctcat cgctggactc cattgccgaa agttttgtgc
   110941 agcataccgt tgagttgatg aggcaaaaag ggaaaaccgt aattgttatt gcccataggc
   111001 tatcaaccat tgctatggcc gataatatta tagttcttga caatggcaag gtcgtagagc
   111061 aaggagtatt taaggaacta atacatagta atgggcagtt ttgccgcatg tggcaacatc
   111121 aaacagttga aaaacatata attgaaaatt aaaaaagtta tgaaattttt tgagtactta
   111181 gtgcgtatcg aaaaaatgca ctgcttaatt aaggaacagc gaaccggcac tcctgatgag
   111241 tttgctgcaa aactaggaat aagtcgaaca aggctttacg aagttattga tgagttaaaa
   111301 tcgcgaggag ctccaattgt gtactctaag tcacgtgaaa cttttttcta tgagtactct
   111361 tttgatatat cgttgaaatg caccatgaaa ccccttagcc gaaaagaatt agttgaaaaa
   111421 aatgggggcg cttcacttcc gttcctgttt tccggacgga acgcacctaa ctttgcaact
   111481 gtaacgttac aaaaacacaa ccaaatttgg atggcaaaat tactaacctt ttaaaactaa
   111541 attaaaatgg aacaactatc tttgaacaaa tttggcgtac aacaattaaa tgccaaagaa
   111601 attgttgaga ttgaaggggg taaccctttg ttgtggttat tagcaggagt tattgtaagc
   111661 gaacttctgg acaggaacgc acctgcagat ttttgggaag gatggaatga tgctaaacaa
   111721 aataaaaaat aaataggtat gaacacacaa aagaacccca cattgcaaaa aattaaagca
   111781 agagaaatgc agtatttgga accacttaat gctacagata tgttaaacat taatggtggg
   111841 acatggttat catactggct tggttatata ttatcttcaa tagataacaa tgtaccaaat
   111901 gaagatctaa ccagatatgg aggtgcatat ttagaaacga taagcggtcg aagttcaatt
   111961 atgtaatttt tttcatttca gataattctt cattattttt gaagaattat ctgattttat
   112021 aaaccaaaac ccacattgta aatgaaaaat aaatcacttc aaattatcat catatgcctg
   112081 ctaatagtag caataatatt ttggcaaata tatttttacg aaagaaattt ttactccgta
   112141 atatttttct tcattactat gttaatttac ttaggcgtgt cgctgtttaa cgcaaagaga
   112201 gccctctcct ctgtagtttt tgttgcaatt ggcatagtca ttagtattct gttaagacgg
   112261 aattttgata ttaacgagtt atcgtggtct ttattggcat tatcattagg cgtattggtt
   112321 tcatatttat catataagag cgtaaatcga taaaataaaa actgaaaatg ccacgttaag
   112381 tcactgctgt aaaaaaataa aaaggaacga accctcgctc ggcgaagttt agagtagcgt
   112441 aacttcgacg agcatgggca catatgagtt tactacacta ctaggaataa gtcgaacaag
   112501 gctttacgaa gttattgatg agttaaaatc gaatggtgcg ccaattgcat actccaaaac
   112561 aagcacaacc ttttattacg agtacccttt cgatattttg gtgggttgta ccatgaaacc
   112621 ccttagccga aaagaattag ttgaaaaaaa atggaggtgt ttcacttccg ttcttgtttt
   112681 ccgtacggaa cactcctaat tttgtaactg taacgttact ctgctaacct cggcaggata
   112741 aagaaagagg gtttgatttt ttaaacaata aaaaatattt ttgctatgga acttgaaaag
   112801 tttggagtag ttgaaattaa tgctacggaa caacaagatg aaaatggtgg ctttttatat
   112861 acattagcag gtggtttaat tagcaatgca attggtgttg ccgcttgtgt aggtgtagta
   112921 gttgttgctg gttatgctat ttatgcaggt tttgaatatg gttatcaagt tgtacaaaat
   112981 tcttaaaaac aatggaaaat ttgaataata ttggcatgtc agagatttct atggaagaat
   113041 ctttagaaat caatggagga aatccaattg gtgttctcat agcaattggg ggcgtatata
   113101 ctggactgtt tgcgattgga tttgctgcgg gtgttattta tggtttagtt gaaaaggcaa
   113161 cagagtaaaa aacattatgg ttctcctttt tttaaaagga gaacctctta tatgctttat
   113221 attatgaaga taccattaaa gatatttggg attaagttta ttgatattat tattaatatc
   113281 gtattttttt ttgttggtct ttttgcaatt gggtttgcaa taggcgtagg gtatgcttta
   113341 atagttagtt tttattaact attttaacaa ccatccctca taggtgtttt attacattcg
   113401 tatataaggg aaaagtctac tttctgggat tttcctatct tttttatttc tctctgttta
   113461 cactagtgca ttggacatgg aaatataatt attaaaaagt taatagtttt taattagtta
   113521 aatcaaatta ttactaaaat ttcgattggt aataaagata attcgttaaa tctttctgca
   113581 agtaaaagcc ttagagtgca atattgcatt aatgctgatt ttatttagca cttcattata
   113641 ccttgaggca ttgtgaaata atgtaattac tcagcaaagt tcagtttagc aaaactttat
   113701 tgtaaataat ttttagcttc tgacagtagg aaataaaatt ctgctcgaga tagtttacat
   113761 ataccctaaa cggaaaactt accgattgta aagtatgtgg gcagagtc
//
LOCUS       DAOSAL010000002        76455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_257_length_76455_cov_50.565353, whole genome shotgun sequence.
ACCESSION   DAOSAL010000002 DAOSAL010000000
VERSION     DAOSAL010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 76455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 76455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76455
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_257_length_76455_cov_50.565353"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(322..1614)
                     /locus_tag="PK049_00550"
     CDS             complement(322..1614)
                     /locus_tag="PK049_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOK60075.1"
                     /translation="MYSELVSKKEKLAVIGLGYVGLPIALEFARKLSVIGFDIKPDRV
                     ELMKKGIDPSRELEPEAFKGCDIEFTSNIDDLRKAKFYVVAVPTPIDEHNLPDLKPLL
                     AATRTVGQVLKKGDYVVYESTVYPGCTEEDCVPLLEELSGLKYIQDFKVGFSPERINP
                     GDKEHTLTTIKKITSGCDADSAEEIAKTYELIIKAGIHRASSIKVAEAAKIIENTQRD
                     INIAFMNELSIIFNRMGINTYEVLEAAGTKWNFLKFFPGLVGGHCIGVDPYYLVYKAK
                     ELGYHPQIITAGRFINDSMGGYVAKQTVKKIIAADKNPKESRVLILGVTFKENVSDIR
                     NSKVVDIYKELKSFGIQNIDVVDPYADSHEVKEEYGFDMASAPNGKYDAIIVAVSHNQ
                     YINLNEEWFKNLANPSCVFVDVKGIYRGKIHNLTYWSL"
     gene            complement(1617..2564)
                     /locus_tag="PK049_00555"
     CDS             complement(1617..2564)
                     /locus_tag="PK049_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOK60076.1"
                     /translation="MKNFALIGAAGFIAPRHLKAIKETGNNLLAALDKHDCVGIMDSY
                     FPEADFFVEYERFDRHIDKLKRQGVKVDYVSICTPNYLHDSHIRFALRQGANAICEKP
                     IVLNPWNIDALAEIEKETGKRISTILQLRLHPTIIELKKQIDSAPKDKVFDIDLTYIT
                     SRGRWYFISWKGDTQKSGGVATNIGVHFFDMLSWIFGSVKSSVVHVSHPDKAAGYMEL
                     EKARVRWFLSVDYNDIPDNIKQKGQRTYRSICIADNELEFSEGFTDLHTASYREILAG
                     NSFGLSDARASIETVYTIRNMAPVGLTGDYHPFCKTLNL"
     gene            complement(2568..3146)
                     /locus_tag="PK049_00560"
     CDS             complement(2568..3146)
                     /locus_tag="PK049_00560"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOK60077.1"
                     /translation="MEKTYFAHETAVIDEGCTIGKGTKIWHFSHIMSGCEIGESCNIG
                     QNVVVSPGVKLGRNVKVQNNVSIYTGVICEDDVFLGPSMVFTNVINPRSAINRKSEYL
                     TTLVKRSATIGANATIVCGVTLGEFSFVGAGAVVTKDVKPYALVVGNPARQIGWMSEY
                     GHRLEFNSDGIAVCPESGQKYKLENNQVFRID"
     gene            complement(3168..4301)
                     /locus_tag="PK049_00565"
     CDS             complement(3168..4301)
                     /locus_tag="PK049_00565"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOK60078.1"
                     /translation="MNIQMCDLRGQYLKIKSEIDAAIQEVIDSTAFIKGKEVSLFQEE
                     LASHLGVKHVIGCANGTDALQIALMALDLKPGDEVITPDFTFIATVEVVALLGLTPIL
                     VDVDPSTFTIDTKRLEEAVTPKTKAIIPVHLFGQCANMDEIMRLAQKYNLSVIEDTAQ
                     ALGADYYHSNGRVSKAGTIGTIGTTSFFPSKNLGCYGDGGALFTNDDSLANEIRSIAN
                     HGMKVRYHHDRIGVNSRLDTIQAAILRVKLKYLDFYNKARIEAANAYDSALKPCSKII
                     TPARAKWSSHIFHQYTLKLNNVDRERLQKDLAAKGIPTMVYYPIPLHKQKAFEPYREF
                     NYGRSFQVSDFLSSSVISLPMHTELSADVIKYITETLLETLKD"
     gene            complement(4409..5944)
                     /gene="rny"
                     /locus_tag="PK049_00570"
     CDS             complement(4409..5944)
                     /gene="rny"
                     /locus_tag="PK049_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005790424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOK60079.1"
                     /translation="MVLTIIIAVSAFSLGGLLSYFSWNKLLASKRNKIIQEAEAEAEV
                     IRKEKILQAKEKFLQLKTEHDKVISEKNNRIAQAETKIKQRELQLSQKFEELQRKQKE
                     VDVIRENLSSQMELVEKRGEELERMHKDQIERLEAISGLSAEEAKAQLIESLKAKART
                     QAMSYINEIVDEAKMTATKEAKRIVVQSIQRVATETAIENSVTVFNIDSDEIKGRIIG
                     REGRNIRALEAATGVEIIVDDTPEAIILSAFDPVRREIARLALHQLVTDGRIHPARIE
                     EVVQKVQKQVEEEIIEVGKRTAIDLGIHGLHPELIRLVGKMKYRSSYGQNLLQHSREV
                     ANLCAIMASELGLNPKVAKRAGLLHDIGKVPDDEPELPHAILGMKLAEKYKEKPEVCN
                     AIGSHHDEVEMTTLIAPIVQVCDAISGARPGARREVVESYIKRLKEMEDLALSYPGVL
                     KTYAIQAGRELRVIVGSEKVSDEEANKLSFEIAKKIQDEMTYPGQVKITVIRETRAIS
                     YAK"
     gene            complement(6178..6486)
                     /locus_tag="PK049_00575"
     CDS             complement(6178..6486)
                     /locus_tag="PK049_00575"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOK60080.1"
                     /translation="MDEKLSINVNVAERLYPLKIDRDDEEKIRKAAKLINDKVMLYKQ
                     RYSDKDVQDYLAMAALQFVIKLIECEDKTDTSEFMSELKDLDEWLGSFIGKQNMSSFK
                     "
     gene            complement(6503..6733)
                     /locus_tag="PK049_00580"
     CDS             complement(6503..6733)
                     /locus_tag="PK049_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60081.1"
                     /translation="MYEREKQENDRLKAQVAQLEAKLKAKEQACAELEERYSRLKIAK
                     ALKASDADVHEAKIKVNRIVREIDRCIALLNK"
     gene            7005..8696
                     /locus_tag="PK049_00585"
     CDS             7005..8696
                     /locus_tag="PK049_00585"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOK60082.1"
                     /translation="MRLFRFAGLLFLSSVTIAQTQPFKLPLEMPSAFSGTFGEVRSDH
                     FHSGVDLRTMGQVGYSVFAIEKGFVSRIKVSPVGFGKVIYIDHPNGKTSVYAHLDRFV
                     GKIAEYVENAQYQKQSFEIELFPNPGELNVNKGDIIGLSGNSGSSGGPHLHFEIRETT
                     SQHILDPFEFFNEWRQTDYNPPTINRLLVFEIDSLRYLLDSLTYKAYEAVKKGSDFIV
                     PLPIKASGKVGLALDYYDIVNSNSLRAGIKKAEVRINGNKTFTFRVDRFSFLETRYAN
                     GFNGITDNGWNGYKAIRLWTDPNNRFSGINHVINHGIIDIAKDSSYTIDVTVWDHSGN
                     SSKLIFKILGVENKVNATSVPSLPLFRWNAENTYKTDSYMVRLPEYALFHDILFQMEE
                     NQSDNLPYPTVTIHNPKTKLFKKFTLEYKASKIPENILSKHYIAKLNGKGFEYVGSEL
                     INGSLKATCNCFGKYTVLVDTIPPSIIAQNLMPSAKITSQNNINFKLIDNTGIKTYNG
                     YIDGNWVLFEWDPKTGILSHNLNPQRIKKGQWHSLKIVATDMLNNDSEYAIKFFW"
     gene            8730..9614
                     /locus_tag="PK049_00590"
     CDS             8730..9614
                     /locus_tag="PK049_00590"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOK60083.1"
                     /translation="MITVFYFILFLISIVIVAIIPWGLIYGYSNLMAKLLHNVLKYRV
                     RVTRSNLRLAFPEKSDAELAVIEKQSYQNLADISVEALKGFTMRNSAIRERHKILNPE
                     LLDYYYNQKQSIIGVAGHLNNWEWGALSAGIQLKHHPVAIYKPLSNKLIDWFIKWNRS
                     IRGTELVPTNQTFDTFQKYKHKPCIYILVADQSPSNLSKAYWINFFNQDTACIHGPEK
                     YAKMYNYPVIYIDIKRVKRGYYTVELSVLCDKPNELKDGELTAAYMHRLEQSIRENPE
                     SWLWTHRRWKRKRPQKAQ"
     gene            9671..10366
                     /locus_tag="PK049_00595"
     CDS             9671..10366
                     /locus_tag="PK049_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60084.1"
                     /translation="MKRYTSVLLAITLFFFSTQVFAQKYRNKEVGLIYFKVNIDPGYH
                     AKFSEYAHLFREPVKPQQDRIVGHLTADIWDIIKQQLTNDVGMIILPLDAYGDKFSYD
                     HYGFPNVTINRALQKGNSKFYISIDVEIGPEFMSKYVTKTSSDTSPQVIQLAPNEIKP
                     KITIKMNIYNDKGIIPVANTKGEAIESKVITLNDKYFDGFVNPNINSPNETLYKLVNQ
                     AVKELVISVYSRK"
     gene            10366..11445
                     /locus_tag="PK049_00600"
     CDS             10366..11445
                     /locus_tag="PK049_00600"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF007144.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HOK60085.1"
                     /translation="MHSPNKSSGYRMIGLMSGTSLDGLDICYCFFQNINSKWQYYILA
                     AETIKYPTHLINQLKEAHNYTAEEITALDFSYGKYLGEQVKRFVSEHGIEADYIASHG
                     HTVFHNPTQGYTLQIGKGAAIAAYSGIPCISDFRSGDVSRGGQGAPLVPIGDKMLFQE
                     FDICLNLGGIANISYDDNFSNRVAYDICVCNMMLNHLAGLTGNEYDFNGDLGRTGQVI
                     PGMLQELENIEYYIQPPPKSLGREWFESYIMPILNRYNAYPLTSQLRTAYEHISGRIA
                     HDCNSLKKTTMLATGGGAKNAFLMELIKQKTELKIVIPDSKTIDYKEALIFAFLGVLY
                     LLKEPGAIASVTGANNNSIAGCLYS"
     gene            11528..13282
                     /locus_tag="PK049_00605"
     CDS             11528..13282
                     /locus_tag="PK049_00605"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOK60086.1"
                     /translation="MRKLTIQIFAAIALLINANTGNACTNYLVTKGASVDGSTMITYA
                     ADSHYLFGELYFKPAAVYPAGTLMKIYEWDTGKFLGEIPQTLQTYSVVGNMNEYQVAI
                     GETTYGGREELQDSTGLIDYGSLMYIALQRSKSAREAIKVMAELVEKYGYYSSGESFS
                     VADKNEVWIFEMIGKGTDIKVDKKTKKAYNANRGAVWVAIRIPDGYVSGHANHARITT
                     FPLENMKTSISSKNLDKIFEPDVEVVYAHDVIGFARQKGYFTGKDEEFSFSDVYAPLD
                     FGAARFCEIRVWSFFKAVNKDMWNYFDYAKGHDLKNRMPLYIKPERKLSVRDMMNFMR
                     DHLEGTELDMSKDPGAGPHGLPYRWRPLTWKYEGKSYFNERATATQQTGFSFVAQMRS
                     WLPDAVGGIFWFSVDDAASTVYFPVYCSVTSVPEAYAEGKGDMLTYCDDCAFWVFNKV
                     SNFAYLRYDLMIQDIKRVQDELENRFISQTAVVDQTALKLLETNKEDAVRFLTDYTVN
                     TGNYVVARWEKLFQFLLMKFMDGNVKQEDGKGGLKYNQYDYCPAPVKNPQYPDWWKKN
                     VIDATGDKLAVPEGAASH"
     gene            13467..14180
                     /locus_tag="PK049_00610"
     CDS             13467..14180
                     /locus_tag="PK049_00610"
                     /inference="COORDINATES: protein motif:HMM:NF021443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HOK60087.1"
                     /translation="MEKIENSPKTDYSRLVLGFLLIVLAGLLLIDNFDLYPVEWGKYI
                     FTWQTLLIVIGLIILPGKGNRTTGTVLIAIGAFFLAAKFYELPVSISKLFWPAVILFI
                     GLSLIFGSRRRRWHPYKTGEVKDDLIDDVSIFGGSDQKFTSQQFRGGKITNIFGGSTI
                     DLSSAKLESGLNVVDLLCIFGGSKLIIPSEWKVKIEVTSIFGGVSDKRKIPQALPENA
                     PELVLKGLVLFGGVDIKSY"
     gene            14334..16391
                     /locus_tag="PK049_00615"
     CDS             14334..16391
                     /locus_tag="PK049_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007762185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOK60088.1"
                     /translation="MITNELINPKSIVVVGGSDDIQKPGGKVLKNLIDNHFKGSLYVV
                     NPKADEVQGVKSVRDVADLPQVDLAILAIAAKFCPQTVEVLATQKGTRAFIILSAGFH
                     EESEEGAKLEQQIVDTISRTGGCLIGPNCIGVMNTNYAGVFTTPIPRFDPKGVDFISG
                     SGATAVFIMESSIPRGLTFNSVYSVGNSAQIGVEEVLEYLDNTFDPQSSSRVKLLYVE
                     SINKPLKLLKHAQSLIRKGCRIAAIKAGSSSAGSRAASSHTGALASSDVAVEALFRKA
                     GIVRCYGRDELATVASIFMHPELQGKNIAVITHAGGPAVMLTDSLSNNGLEVPPIEGP
                     KAKELLAKLFPGSSVANPIDFLATGTAEQLGFIIDACENDFHNIDAMIVIFGSPGLFP
                     VYDVYDLLDEKMKVCRKPIFPVLPSVMNVKNEIEHFIAKGRIFFPDEVSLGNALAKIY
                     HTPKPSPEAPVLPKVNEKAISEIISKSNNGYLTPDAVQQLLDAAGIPRAGEAVVTTAD
                     DAARAAEKLGYPVVMKVVGPVHKSDVGGVVLNVKDSETVRKEFDRMIKIKDTTAILIQ
                     PMLKGVELFVGAKREDKFGHMVLCGLGGIFIEVLKDVKAALAPIDTNEALNMIKGLKS
                     YGIIKGARGQEPVNEQIFAEIVSRLSALVTVAPEIFEMDLNPLLGSKDKVVAVDARIR
                     IEK"
     gene            16398..16547
                     /locus_tag="PK049_00620"
     CDS             16398..16547
                     /locus_tag="PK049_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60089.1"
                     /translation="MSLKEKWNSLTANEKMMIGLAIILVILIATRFEYIWDQVKAGFA
                     RYFNR"
     gene            16584..17255
                     /locus_tag="PK049_00625"
     CDS             16584..17255
                     /locus_tag="PK049_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mpv17/PMP22 family protein"
                     /protein_id="HOK60090.1"
                     /translation="MVGTILFLLPFFISETVYNFYVDFNASHGMVTSFIKFALLATFG
                     EAIGLRIRTGNYNQPGFGLLPRAVVWGLLGLTIKLAFVLFASGVPQFLAYMGMENAPQ
                     ALKGELTLAKILVAFSISLFMNIIYAPVMMTLHKITDTHIVNNGGTLSCLLKPIHFGN
                     ILASINWEVQWSFVFAKTIPYFWIPAHTITFLLPADFQVLFAAVLSVALGIFLAVASL
                     KGSGK"
     gene            17303..17821
                     /locus_tag="PK049_00630"
     CDS             17303..17821
                     /locus_tag="PK049_00630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HOK60091.1"
                     /translation="MNKRIGIFYGPTGGSTEKVAQLIAKELGAIADLHPIKESTPSMV
                     NNYDIIVFGCSTIGSETWNGEASRSDWDHFRPELKKIQVQGKLFAFFGTGDSVTYPRN
                     FVDGMGILAKELIAQGAQVIGQWPTDGYSFTDSEALINGKFIGLPIDEDYEADKTIAR
                     VKGWVELIKKSI"
     gene            complement(17879..18667)
                     /locus_tag="PK049_00635"
     CDS             complement(17879..18667)
                     /locus_tag="PK049_00635"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOK60092.1"
                     /translation="MKRLFWVLTSVILLSSCSDDGINFFTLEQDIQFGAQLDSAILAN
                     PNEYPILDRTQYAEAYTHIERVMNAILSSDDLRYTTTFPWQVKIIHDDNVLNAFAAPG
                     GYLYFYTGLIKFLDNEAQLAGVMAHEIAHADRRHSTQMLTRQYGFSMLLSIIMGDNPS
                     QLEQILSQLALGGTMLKYSRDNEYEADKYAVTYLCDTDYHPRELAGFFEKMMGSTQKI
                     PEFLSTHPSDENRIKNIDDVWRSLGSKVGNTYADRYQSFRNSLP"
     gene            18865..19554
                     /gene="mtgA"
                     /locus_tag="PK049_00640"
     CDS             18865..19554
                     /gene="mtgA"
                     /locus_tag="PK049_00640"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HOK60093.1"
                     /translation="MLVRVITYSVVLFFTLSISLTVVYKWVNPHYTWLMILKKHLPET
                     QGGSSNFEQSWVSIEEISPQMVLAVIAAEDNKFMEHNGFDWESIKKARKYNQRGKKLR
                     GASTISQQTAKNVFLWPKRSWVRKGLEAYFTLLIETFWSKERIMEVYLNVAEMGRGIY
                     GVEAAARKYYGKPASRLTRHEAAMIATTLPAPAKRNPSKPSAYMVSYQRKILWNMYNL
                     GEIDINEKEQK"
     gene            19577..21508
                     /locus_tag="PK049_00645"
     CDS             19577..21508
                     /locus_tag="PK049_00645"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOK60094.1"
                     /translation="MQRLLFPFWLIVTFLVCFTPSKAQFNKAYFFYKGEELISRGNYA
                     DAIPVLNTLLNVDSTIHEAWFLRGVARYYQNDLVGAGNDFSKCLNINPLFSQAYQYNA
                     MVLQQLGKSVESIECIQKAIELRPASAQYLFTYGVILFQQEQYHDALYAFNQVLKIDD
                     RIPDAWVNRGTTRLMLSDSSGALTDYSAAINLNPFNANPYLRRGALYAQQSNYPLALQ
                     DLNQAIGIDSTLAQAYFTRALVFYYQKHYSQALSDLNKVLQLEHRNTLALFNRAIISY
                     EVGNPESAIDDLTRLSSIIPTNVLVHYYLASIRYELGYLPMALENINKAIAIFPEFAN
                     AYRLRAEIKTKKGDLKGAEADIAQGNRLAQAYSNRAINDIASVIDSTGKLKRLLSLDE
                     ELDITSSISMEQLRTRILTIVYPIITLKVDKIENVKPRNWQSSNLRQILATQSCRGYS
                     LFFEYDTINQHSQLETIATDSLSEICKAIAHLQGNYYTAAMKEFSETLKADSSNTLYR
                     LAEVLAKIKMTQFVENVKNKSLQSGKSNISQKTIDNKPYITAGNDLKTIALDNDFEGI
                     VPYNIGVMFMHTENNDLAIEWFSKAISINPLIHGAWYNRGLLYLINNETVKGCADLRK
                     AVNIGNNQASEVVARFCKK"
     gene            complement(21558..22985)
                     /gene="hydG"
                     /locus_tag="PK049_00650"
     CDS             complement(21558..22985)
                     /gene="hydG"
                     /locus_tag="PK049_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HOK60095.1"
                     /translation="MKFNPEKYRIPDERMKPFIDADEIWEYINNAKPTKERVREIIAK
                     SLDKNRLNLEEVATLVNTTDPELVEEILQGARTLKERIYGNRIVLFAPLYIGNKCTNN
                     CQYCGFRASNKDAIRMTLSDEEIVQEVEALEDNGQKRLILVYGEHPEYSAEYIAHTVR
                     LVYGIKKNNGEIRRVNINAAPLDIEGFRKVKEAGIGTYQIFQETYHPEAYTWYHPSGK
                     KKDFEWRLTSLDRAQEAGLDDVGIGALFGLYDWRFEVLGLVRHTNHLEACYNVGPHTI
                     SFPRIKSASGLNITDKYFVSDSDFVKLVAILRLAVPYTGMILTAREPADIRRQVIQFG
                     VSQIDGGTKLELGSYHASKNEHQDLNKEQFEINDNRSLGEIIDELIDHNYIPSFCTAC
                     YRLGRTGEHFMEFSVPGFIKRYCTPNALLTLTEYLVDYANPEVQQKGYKLIEKTLGEL
                     DGHQNTLEIRRRIERIKAGERDLYF"
     gene            complement(23069..23317)
                     /locus_tag="PK049_00655"
     CDS             complement(23069..23317)
                     /locus_tag="PK049_00655"
                     /inference="COORDINATES: protein motif:HMM:TIGR03959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HOK60096.1"
                     /translation="MEKRIGAALILVENRESASRLNQILSAHADIIIARQGIPLQQRG
                     ISVISLVLEGTTDEIGALTGPIGKLSGVQVKTLMLKGV"
     gene            complement(23487..24431)
                     /locus_tag="PK049_00660"
     CDS             complement(23487..24431)
                     /locus_tag="PK049_00660"
                     /inference="COORDINATES: protein motif:HMM:NF018299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HOK60097.1"
                     /translation="MKVHYGFDGIGEITNPVVTTGSFDGVHLGHKVIINRINEIAQSI
                     GGESVLITFYPHPRKVLYPETAGKNLMFILSQREKIELLSKTGLDHLIIVKFTLEFSK
                     ISSVQFIRDYLISKLNARYIVVGFNHHFGHNREGDYEELKKLSSEYNFMVEEIPEQDI
                     QQETVSSTTIRKALLEGKIQRANAYLDHYYMIIGALGKGSHFFGEVGFPTLTVQIEEA
                     GKLIPPEGIYAVSLEWNKSSYRAMVIIWSNGDNTDLNPVLTRNVELHILNFDGMLHSS
                     DAYIYFHKQIADRIDISDNRLLFQQLTQAAKQVDELIY"
     gene            complement(24441..25076)
                     /locus_tag="PK049_00665"
     CDS             complement(24441..25076)
                     /locus_tag="PK049_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOK60098.1"
                     /translation="MDKANLPEKTIERLSKYRRTFLNCLTRGKTHIYSHELAGLHNIT
                     AVQVRRDLMLIGYSSMKKKGYDAQELIKVIGDIIDHKVGLNVAVVGMGNLGRAITTYF
                     NGKRPKLNIVAAFDVDPNKVNRVISGVNCYHMKELPDVIKRNDISIAIISSPPDTANQ
                     VAEQLVAAGIKGILNFTTIPLVVPENVYLEEYDMITSMEKVAYFVKQGYSR"
     gene            complement(25156..26304)
                     /locus_tag="PK049_00670"
     CDS             complement(25156..26304)
                     /locus_tag="PK049_00670"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HOK60099.1"
                     /translation="MEVVDILVVDDEPGIRSGVTRILRNFTVSYPFLEDDIGFNVIEA
                     ATGEEAIDIIKRSSPAIVLLDNKLPGIQGVEVLQFINQNHPDILVMMITSYASLELAV
                     RATNIGTYDFVPKPFTPQELKSSIENITKHYFLRRMTRKLHREGKQVRFQFLTVLSHE
                     LKAPLNAVEGYLKMMQEKQAGESIDSYMDFIDRSLARIQSMRNLIMDLLDLTHIESGK
                     RQRTLREIDLTLVARSAIETMTPLAIQRDVNLSLQTTEPVIMLADSDDMEILFNNLIS
                     NAVKYNKPGGWVKCSISKTASEVTLVVSDSGIGIEPEDIPKLFQEFTRIKNPQSKNVT
                     GSGLGLSIVKRVVDLYGARIDVQSEPGAGSTFTITFPLPGSDTLNDGI"
     gene            complement(26307..26702)
                     /locus_tag="PK049_00675"
     CDS             complement(26307..26702)
                     /locus_tag="PK049_00675"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOK60100.1"
                     /translation="MANSRKTILIVDDDTDYLFQLKFHVEKMGFDVVTAESQREAENI
                     LKNIKPDLAILDLMMENEDSGFILSYKLKRMYPDVPVIIATAVSAETGMSFGISSEQE
                     RQWIKADLYLEKGIRPDQLHREILKLLKV"
     gene            complement(26718..28727)
                     /locus_tag="PK049_00680"
     CDS             complement(26718..28727)
                     /locus_tag="PK049_00680"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOK60101.1"
                     /translation="MEMNREKRKLVYTIKELCRVCYTCVRECPAKAIKIVNGQADVIS
                     ERCIGCGNCVKVCSQDAKVFLLTRTEVKSMLQSGERVAALVAPSFPAEFTEIDDYRYF
                     VGMLRALGFSHVFEVAFGADLVASRYKSLLEDRSGKGYISSDCPAIVTYIRYYHPDLV
                     ENLAPVVSPMVAISRVVRQIDSNLKQVFIGPCIAKKDESIEIDEAITFRELRVLFEQA
                     SITPESVEPSDFDPPHAGRGAIFPVSRGLMQTVNIFDDLIEGNITVAEGRIGFQEAIK
                     EWESGNLKGQNLELLCCEGCIMGPGMSPGGKRFERKTYISSYARNKVTPEQLKEWELA
                     ISKFKDLDLTRSFNPDDKRIAKPTDDEINKVLAKMGKFTPRDHLNCGACGYDTCTEHA
                     IAIVEGLAEVEMCLPYAIEELHESINDLAISNEKLAKMQQALKHSEKLAHMGQLSAGI
                     AHELNNPLGVVLMYSNILLDECKPDDPLRKDLELISSQAERCKKIVSGLLNFARKNQV
                     KVEKVNVKKLAEDSIAGVVIPKNVTTNVVCRTTNLMASLDYEQMMQVLTNLNKNAFEA
                     MPNGGDLTIVIDGDDHQISISVIDTGEGIPEENMDKLFTPFFTTKGIGKGTGLGLATT
                     YGIVKMHKGKIDVESNTDPQKGPTGTTFRITLPRNAENELGNDNS"
     gene            complement(28749..30473)
                     /locus_tag="PK049_00685"
     CDS             complement(28749..30473)
                     /locus_tag="PK049_00685"
                     /inference="COORDINATES: protein motif:HMM:TIGR02512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HOK60102.1"
                     /translation="MNITIEVNNRTIQAHKGDTILEALNRNGIRVPTLCNMTDLTPSG
                     ACRLCVVEVEGRDNLVTSCSHVVENGMRIMTHSPRVVRARKTLVELLLANHPDDCLYC
                     ERNGFCELQDLAAELHIRERKVLRKSFTQKLDLSSPSIVRDPAKCILCGRCIRVCDEV
                     QSVSTFEFVNRGCNMYVGTTLSRDLNFSNCIGCGQCVLVCPTGALHERQNVAEVMDAL
                     SNPDLIPVVQIAPSISVSVATEFGLKPSKDVNGLLYNALRKIGFKYVFDTAFAADVAA
                     IEMAAELIERKGNGSNLPMFSSCCPAWVKFVEQWYPDYIPALSAVKSPQQVMGSLIRN
                     YFAAENELNPSKIFSVSVMPCLAKKFEAQREEMTTRGLSDVDTVLSTRELVRLIKLYG
                     IDIQGIESQHPDKPFNIRSSSGKLFGISGGSAEALARVVYHKLTSKELTDSKVNEVKI
                     LSGVKAFSFNAAGQTFTFAVVNGLKNIHSILPVLMGSDVKLDYIEVMACHGGCVNGGG
                     QPYVDEEKYVKLRAKAIIEIDEAEGIKCPTRNPAVQSIYDEFLGETGSEKAKKFLYTR
                     YSKREVLL"
     gene            complement(30487..32436)
                     /locus_tag="PK049_00690"
     CDS             complement(30487..32436)
                     /locus_tag="PK049_00690"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOK60103.1"
                     /translation="MARCDSKAEISFIKDAVIPNLHDGVWLTEVNAKLSALRHERVRQ
                     PIIYVGVTTSSIVAGALKTRSAIEQYLDDYSVDADVICVGSHGLCSLEPIVEVQMPGR
                     TRVAFANVTPERVNSILDGALNGFIQPENAIFQYYSEIHEPWHGIPFRNELPFFKRQN
                     RVLLKNAGIICPDSIAEYIAHGGYSALAKSIRKYTYDDICRIVEESGLRGRGGGAFPT
                     GTKWRKALQVATSDRYLICNADESDPGGFMDRLLIESDPHRVVEGIILSSYAVSASKA
                     IIYIRDRYKITIQRIQNAIDQAYEVGLLGYDILGSGYSLDIEVRKGPGAYVCGEETAL
                     IKSLEGKRGMPSIKPPYPAEFGLNGKPTVINNVETLANIPEIISNGPQWFASLGTEGS
                     KGTKIFSINGQASQTCMVELTFGEPLGALLDLAGGVKDGRTFKALHLGGPSGVSVTAD
                     ELDAPIDFDELPKRGISLGSGGVQVFDNTVCMVDLVKYFMHFIRNESCGKCIPCREGS
                     NRMYQILDNISKRPVSNEGHNTLERFKGVIQLETLAEVMRDTSLCGLGQTAPKPILKA
                     LKSFRDEFEEHIFDRKCRASVCRDLRTFYIDIERCTGCTACAKKCPTQAIYGTPRSPY
                     FIVEERCIGCGICQDTCKFGAVFFK"
     gene            complement(32439..32903)
                     /locus_tag="PK049_00695"
     CDS             complement(32439..32903)
                     /locus_tag="PK049_00695"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOK60104.1"
                     /translation="MSNAEKIEEILERYSYLGSDSLIPILQDIQNELGSLNEEAIVLV
                     GRKLNMPTSKIYGLATFYNQFKFEAKGKNHICLCNGTSCRLKGAKWNMVYLEDNLKLK
                     NGQTTRNGQFSLEVVTCMGACEHGPVISVNDEYYREVDPEKLKNILNDLTQE"
     gene            33205..33450
                     /locus_tag="PK049_00700"
     CDS             33205..33450
                     /locus_tag="PK049_00700"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOK60105.1"
                     /translation="MSKRAELIICLGSSCFARGNKATLKAIMKFLDDHDLKDKVNFHG
                     GHCFGNCAEGPNISINGKIYTGVDENGIIDILTNELL"
     gene            33459..35330
                     /locus_tag="PK049_00705"
     CDS             33459..35330
                     /locus_tag="PK049_00705"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOK60106.1"
                     /translation="MSQLFRIDEQTCINCYACVRVCPVKAIEVKAGQNYARIIDSRCV
                     GCGSCLNICPVNAVSYRSSKKEVLELLKGDEPVAAICSPSISGEFVDITDYRKFVQMI
                     KQLGFTYVCEVTFGVDLVAREYKKLFEDFKGKYFITSMCPVVVSMVEKYHPEIAQNLA
                     PIISPVVATAMAVRKEYGSNVRVVLIGPCIQSKEEIRLYDDERRIDENLTFVELRELF
                     DEFNIKESNLEYSEFDEPIGYKGSLFPIGRGILQAVDISEDLLTGTVISTNGPNNTLQ
                     ALEQFETSIDLINRHFNLFYCEGCLMGPGTSRGGKKFIRRTMVVNYANKRLKTFDREK
                     WEKNIAKNSLGNFARGFTIDDQRLPTPNEHKLEEVLKVIDKEFVGRDSGCEACGYSSC
                     YDFASAVASGLAHTDMCLNFTLKKRQEYIKTLQATNEKLAKTQEALKESEKKARTEQE
                     AAREATETITAMLKKLPSAVVLIDKKMKILQSNQSFIDLLGDDALEINEIIPGLVGAD
                     LKTLLPYNIHNLFTSVLQNNTDILNRDVMYKDKLLNLSVFTIKPNSIVGAVIRDMYAP
                     EVQKEEVIKRITEVIDKNLAMVQNIGFLLGEGASETEKMLNSIIKTYREGKNNDDKA"
     gene            35343..36524
                     /locus_tag="PK049_00710"
     CDS             35343..36524
                     /locus_tag="PK049_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOK60107.1"
                     /translation="MSSKFFIEVNCQQKNHDEERICGDVFYSRRLQDEERTIIVLSDG
                     MGHGVKANVLATLTSTMALNFTIEHKEVNTIAEIIMNTLPVCSVRKMSYSTFSIVDID
                     HNGETRILEYDNPQCLIMRGTEELEPEWQCIILNSEKNAGKELKFCSFVPQKEDRIIL
                     WTDGVAQSGLGSADYPFGWGDEAREFVKRMVKNEPDISARKLSTKVVNMASLNDGYHP
                     KDDTSCVTIYFRNPRKLLICTGPPYEKENDTKLAKIFDTFEGKKAICGATTVDIIARE
                     LKRSVEDSLEFTDPDLPPISQMEGADLVTEGILTLSKVNTILESYNENTNLGRGPADE
                     LVKLILESDSIEFVIGTCINVAHQDPNLPVELEIRRTVVKRIANILQDKFLKEVKLQF
                     L"
     gene            36658..38937
                     /locus_tag="PK049_00715"
     CDS             36658..38937
                     /locus_tag="PK049_00715"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOK60108.1"
                     /translation="MRSFRFFLLNMLIATYFSAVSYGQDWNSIFTLETDAEYYMAEGN
                     FSKAADTYVKALKKFPESANLKFKIGYCLLKTPDRKSEAISYLEEAVMKISDKYDAKS
                     LKEPNAPPEALYQLGMSYMYQNDFDNALKSFREYQGYIKPKDEHAKLMAEHAIASCEN
                     AKKFQKSPIGIKYNRLGPDFNNENDNFNAVVSGDGSTLAYTTRTSSGNKIFVAKKGED
                     GLWAKPVDITRNLGSKNLTTSYLSYEGTELYLIENDPRNSEIVVSFLQKRKWSSPVKA
                     AKPINSKFNETHVCVTRDGNTVYFTSDRKGGQGGYDIWASTINNGRWSDPINLGPNVN
                     TPFDEATPFLSPDEKYLFFSSQGHNTMGGFDVFFTNLQGSPNVKNAGYPLNTTDDEIF
                     YFPTGLSSGYIAKFSPDGFGKLDIAEVEIIPMVDVKLNLMLADNAPLDKYYNVNVTNN
                     STGEIVEKQSVKGKTQLSLKVKPGDYTVTAQGEGFEETKTPLSIPAKPDKSEYQASLM
                     LNPRVVQQPVAQLEPPKEEPKPEVKPEVKPEPKPEVKPEARATDKPKAVEPEPKKEPV
                     VKQAPKKEPKPEVEKPKPEPKPAKALPPTPPPTAAPTPRMDFNKTSSTRTLAKPTYSV
                     QLMASLYPVDYNYFKLDSISVTISPDGYYRYSVGNTDNIKEIEELQQKVRAHGYSNVW
                     VRINREHPGYTIQFLALQKPKSIKQFTELSDVMVYRGNDGVYRYCVGKYSTPEEASSD
                     LAKITGLGYSGAFVRELGK"
     gene            complement(38990..41224)
                     /locus_tag="PK049_00720"
     CDS             complement(38990..41224)
                     /locus_tag="PK049_00720"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOK60109.1"
                     /translation="MRLIKLFIITVFVLGNNVLAQNIIRGIVYNENKIPLEGANVFIP
                     MLVKGTSTNSSGEFELTGLPNGKFDIQISHIGYASQIVPVTLGKTQFELDVVLIPASV
                     EAERVVVSTGYSTTQHQNAVKIEVLRLNEHHLIKSPNFMELLTTVPGIDMISKGNGVA
                     KPVIRGLSMNDVLVLNNGVRFENYQYSNHHPLGIDEFGIETVEVIKGPASLLFGSDAI
                     GGVLNFIKERPATVGKLEGDYNLQLFSNSLGMVNNLGFKVSDNQFHGGIRVGQKTHSD
                     YLQGGGEFVPNSRFYEHSVKLNGGFNGKMLSTNIYYDYNMQKLGLVEEEAIDLIASRG
                     RKPSLYYQQFGTHLVSQQSKIFLGKTKVDFNTSLQSTELAHLGNPNEYELQMRLTTLI
                     YEAKVLFPSNPGNDYILGAQGVNQWNININNRETILLPNAFSSGYSIFGLAQRSFNRF
                     RFQAGLRFDAKQIKSNEVGIPSEPEYRASVDRKYNNITGSVGATYRLNEYFLIRANGA
                     AAYRNPNLAELTSNGPHEGRYEVGNISLKPEKSVEYDVSAHYHNDNFLADVAGFVNSI
                     GNYIYIAPTGALNGGLPEYRYSQDDAYLYGGELGLHFHPKSVQWLHFKTNYSLVIGKK
                     RNGEYLPFIPANKLTFETGFMGKKFALGSKPYLLIAINYAFAQNNIDPGETVTPDYIL
                     VNLSMGSSFRLGNQPLEVNLTVQNLLDKKYVDHLSTLKEVDLYNPGRNISLSLRIPFR
                     ASIR"
     gene            complement(41428..41751)
                     /locus_tag="PK049_00725"
     CDS             complement(41428..41751)
                     /locus_tag="PK049_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60110.1"
                     /translation="MKKGKTKHSLILLTLFLLPIVSKIGDSLFHHHNHKNEHHNSSIN
                     ITHYHDKCLVFQYELIVFDPKVVLPKVEIYLNIISEISDLITLNVGIDSFELIKIRAP
                     PFNIF"
     gene            complement(41803..42918)
                     /locus_tag="PK049_00730"
     CDS             complement(41803..42918)
                     /locus_tag="PK049_00730"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOK60111.1"
                     /translation="MMKINILLIITFGVLLMSCSGKNNKSDENQPTDSLVVISVEQFK
                     AMNMAIGNPTMVKFASEIAVQGVIEPSPNAKAYITSPIGGVIKSVKVVPSSVVKKGQA
                     LIAIDGPEILNTQTLFVETYNQHRIAKANFERISQLSKEGIASQKELLQAESEYRILD
                     AKLHSHRILLSKLGLNPDEIIKGEFSNEAYLVSPIEGTVARVETSIGKPVTTYEPLAE
                     VINTQSLLLKFFIFLNQVNSIKPGQKVEISLPGGGKVYGSVISVGLDANTENKAVECF
                     ASIILPANLVLISGTRVSAKVFTNFVEGWALPRTALHEIETGHMVYTLNGSDSLSYFF
                     KKVPIQVGLIQDTIVQVFDSTLSNVLVKGGYELTVEE"
     gene            complement(42959..46105)
                     /locus_tag="PK049_00735"
     CDS             complement(42959..46105)
                     /locus_tag="PK049_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HOK60112.1"
                     /translation="MIERIIRFSINSKLIIILLTLTIALFGVYALTQIPVGAVPDITN
                     NQVQVITTSRNLSTQDVEQFITYPVELEMSNLPGVKEIRSISKFGLSVVTIVFEENLG
                     TYLPRQLIAEKLKAAAANIPEGFGEPDMGPISTGLGEIYQYVLDVKPGYETRYTTTDL
                     RTIQDWFVKRQLSGIPGVVEVNTWGGYLKQYEVAVDPQKLAQYGVSIIDVLNALEQNN
                     GVAGGAYIERDNQSFFVRGEGLLKSLADIEQIPISTINGISVRIADVAKVQEGHANRF
                     GAITANGQGEKVMGQIMMLKNANSNKVIEAVKQRVAEVQKHLPEGVYINPIVERSELI
                     SKTTNTILENLIFGCLIVFLVVLLILGNLRAALVISSLIPLALLFTISAMYIFGIDAN
                     LMSLGALDFGIIIDGAVIIVEFIVMKLTLRSNEITGIENTERKKIFDTISFQGASKMM
                     NSAIFGQIIILIVFIPILSLAGVEGKMFRPMALSFSFALLGAMFFGFTWLPVAASLFL
                     RPQKSNWTVTRWVMWLLHSSYKPVIEWAYNNKRWVLGLAFAMLLFSGWVFTRLGAEFV
                     PTLDEGDFVIQPVLKTGTSLTRTTELTTMMEKILIEKFPEVDKIVSRIGAAEVPTDPM
                     SMEEIDMIIKLKPKKYWVSAKSKEELANRFKEALSVIPNVEYEFTQPIEMRFNELITG
                     VRADLAIKIFGEDLGVLNAKAIEVTKLIDGVPGAADIIMDKTAGLPQISIRYNRDKLS
                     VYGISVTELNRVVSAAFAGATAGNIFEGEKRFDLVVRLSDSYRHDIDNVKNLLVSVPG
                     GGLVPLGELAQVEYSKGPAKISRDNTHRRVVVSVNVRNRDLESVVKDIQKILDSKLRL
                     PAGYYVEYGGQFENLRNARKRLLVAVPVALALIFIFLHFAFHSLKDAILVYTAVPLSV
                     IGGILMLWFRGMPFSISAAVGFIALFGVAVLNGIVLIEHLKELKDEGVENLRERIMRA
                     TRDRLRPVLLTASAAAMGFLPMAISTSAGAEVQRPLASVVIGGLITSTLLTMIALPLL
                     YAVFERFNGLKFWNLFRK"
     gene            complement(46173..47783)
                     /locus_tag="PK049_00740"
     CDS             complement(46173..47783)
                     /locus_tag="PK049_00740"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOK60113.1"
                     /translation="MEHGIAGVFHYDGSNIKQIKETDGVSSNLIYVLYADSDYLWIGT
                     NLGLDRLSLAEYSKGKIDLRHYGSSEGLPDLEINLNGVLADGYDGFWIATNGGLAHYQ
                     KSLDKINNIPPKVSITNLLLRSQPTPWADYASDIDELTGLPSQLKLSYWQNHLTFEFA
                     GVSFKNPQGVKYAWFLEGLDNSWVESKNRQAVYSNLQPGKTYRLHLKAANSDGIWSGE
                     VISMPIYIAPPFWATWWFRLLSIIAIGLLIYWYVNRRIQYLKERQEELEAMVEQRTVE
                     LREQLDIVDDKNRQIMDSIMYAKFLQTSMLPSVDDFKKYFNDAFIFYRPKDFVSGDFY
                     WFCHHKNVSVFAVADCTGHGVPGAIVSVICENALRNAVKECDYQNPAQILTITNAHVV
                     EFFTQSQKSIHDGMEIALAIFNHHTLELDFSGAKLPLYYISGGEVVKLKPDIYRIGWD
                     KEKVLYNNQRVKLSKNDLIFAFTDGFCDQFGAETGQKFSSARLKSLLVDNNQQPHTYI
                     EQLLSITFDQWRGFTEQTDDVLVMGIKV"
     gene            complement(47749..49347)
                     /locus_tag="PK049_00745"
     CDS             complement(47749..49347)
                     /locus_tag="PK049_00745"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOK60114.1"
                     /translation="MRFTIVLSLLSLSVVSFAQRYNIEIIGLKTGLLHSLVTGIDQDT
                     QGKVWLSTGGGLCNYNGFEFKYLTTRDGLNYTRLTDVAVDAEGNIWASSSLGLNMVRG
                     NRILTIPKDTIGEVASLGKSSNGVWVLSNRGVFRVAFSKDKFDIIQYSLPVNLNVALS
                     PIFQDRPLTDFVFQSTLNKVYVGHKGTLYKLSNESFSPLTFEPSVYVNACSENANGDV
                     FVATSDGLYRLVDDKPIKVNHPYASRIDILKFVCIGSKIWCNGKLKGSDEVSIHAFDL
                     EDESFNRTLGVKNGLPDIPSKLYVDHEHNIWILTNNGIAILKGEAFTCLTTNDGLIGN
                     KIWGICRVSDGTLWAGTIGEGLSVISPTRIYQYSTKNGLPDNYVGKIFESSSRKIYVG
                     TSNAGLNLATYINSTLGYSFNRLPLLPNDRVRIDDIAEDRQKRIWVATSKGLFYATDG
                     IHFNHFPLFDSDTGGVFVQKLQIDTLRKRMWVGTRFNGIFFIENDKPFKFTGIDKNAE
                     ISTIVQDFKGDIWFGTRNSGGFSL"
     gene            49471..50646
                     /gene="mnmA"
                     /locus_tag="PK049_00750"
     CDS             49471..50646
                     /gene="mnmA"
                     /locus_tag="PK049_00750"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOK60115.1"
                     /translation="MERVVVGLSGGVDSSVAAYLLKQQGYEVIGMFMKNWHDTTGVLK
                     GDCPFDDDKQFAEMVARKLNIPFKFVDLSDQYRQRVVDYMFSEYSKGRTPNPDVLCNR
                     EIKFDAFLEAAIKLGAKYVATGHYCRKETITKDNTTLYRLLAGVDPNKDQSYFLCQLN
                     QQQLSKSLFPIGHLHKPEVRKIAAELGLATAERKDSQGICFVGKVDLPVFLQQKLKAK
                     KGKIVEIPADFYTQLSSINSNDLASLSKPYTYKLSNGKVVGEHQGAHFFTIGQRKGLN
                     VGGKAEPLFVIATDIENNIVYVGMGQSHQGLYRSGLSMNPNEVHWVRPDLALSAGQSL
                     QVLARIRYRQPLQQATLHHTNNAYYLIFDTPQRGVTAGQFAAWYIDDELIGSGVIAE"
     gene            50677..53547
                     /locus_tag="PK049_00755"
     CDS             50677..53547
                     /locus_tag="PK049_00755"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HOK60116.1"
                     /translation="MFKDYHNLIVKALNRQLSFLIVNILLTLSSYTYCQSSLLINNFE
                     KKDYRAGNQNWSICLGMQNHILFGNNNGLLCFDGASWKIKPHPNNTTIRSVLTAKNRI
                     YAGSFEDFGYYEILNNSIGNYVSLAKPFRSSIHNEEFWRIVSIKDIIFFQSFGTIFYL
                     SKDRIGKIPLDFSVLLLMQAGENIFTQAINGPIYRVDTLGLKQIKGSEIFKNTEVKSV
                     VVLNSDTLFLTSTMGIFRFDGNAFTPWKTSIDIQLIKSNPNIAQSQGSKLAIGTILEG
                     VFIFDNKGKLLHHIDSKLIHNNTILSMQFDSIGNLWLGKDKGISYVAFNSPISTYVNE
                     KESFACYSGALSQNKLFLATNQGVYTYSLNPRGEFENQGVIDGTQGQCWFTKQIGNKL
                     YFGLNNGTYSYSNGNLNKVCYISGGYNLERFGIEPSLFIQSTYRNLVTYSYENGSLRV
                     NKIIPTYSSPTRFLQIDFQNYLWLGHTVTGIYRAQISNDLSEIVKIDTIGIKNNLNIN
                     TNRIYKIENRIVVPTQKNILRWDDISEQFIPFNEINYQLQGFEKCKAIISLPANHYWF
                     IKDDEWGLFEIRYGEAKLKYRILPQAYNIEMVDGYENIVSLNDSLQIICLDNGFSILN
                     LKMFDRIPIDIHPPIITEVVIWNGEQNVKSHPRLNRNNELTIPWRKNNVSFSFTSKDI
                     IGVKHFYQYMLDGVDREWSEWTPNTSIDYKRLSPGTYRFKVRTLDSKGHITPNATVTI
                     KVLPPLYASWKAIGFYILLGFAITAYTRARYKKKVKKHYEEKRKLELEKINKEKEEKE
                     KIIIKLQNEKLQADIENKNSQLALSTMAIVRKNQLLQDIAQELEGLKNELGYRIPNKY
                     YSKISRLITQDIESDHDWEIFEKLFDQAHQNFFRRLKENYPSLTPSDLRLCAYLRMNL
                     SSKEIAPLLNISIRGVEERRYRLRKRLGLDTNDNLNDFILKF"
     gene            53980..57006
                     /locus_tag="PK049_00760"
     CDS             53980..57006
                     /locus_tag="PK049_00760"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOK60117.1"
                     /translation="MHSLTQTKPKTLIRIAGTLLLVLLFSSSFAQQIIKGTVKAGDTG
                     EKLPGANVTVKGTTRGTITDLEGNYQIEVKPSDKILVFSFVGFEDTEVEIANQKVIDV
                     TLKLKSTQLQEVVVVGYGTQKAKDLTAPIVTVKGNELSKQVASNPMNALQGKVSGVQI
                     INSGEPGSGAAVKIRGVGSIGDYAKPLYVVDGVFVDNIDFLSSSDVEDLTVLKDASAA
                     AIYGVRAANGVIIVTTRKGKTGIPTVSYDSYVGFQVPVNILKMASKDQYITLLNEANE
                     NVPGYVPKNPADYPTSTDWYQELVRPAGMNNHNLDISGSTDKTSYSIGGNYIYQNGIM
                     DAKNNYQRYNIRGRLDQQVNKNLKIGINTIISNYSRFNPNTYAFFGAYVNPPVYSVYD
                     PTNSDAYPVKFGAPQKYGFGNEYGNPVASAYYTDNFEKGHKEVFSIYAELDIIKDKLT
                     YRAAYNQDQDNWNQRSYTPQFYVGGSQGVTKSSLTKTFGGASKQIIDNLLTYKNSAGK
                     TSYSILLGHSIRMEKWETLSGFANSVPGYDEQSKYLINGSFRDRNAYDGAARYNGLSF
                     FTRGTLNHNDKYLATLTFRADASSKYQQKWGYFPSLGIAWNITQENFSRINEKFANLK
                     LRLSWGMLGNDNVPANSSMILGQTGVGSSGVFGDNLVDGMGAQTVLQNYLKWETVTEF
                     DMGFDFTLKNQKVSGELDYYRRVTNNVVFYAPIATGGGIAELLGNNGKVLNTGVEFSL
                     NYKNEISKDLNYNISFNATFNHNEVLELNGREYIPSGYVRGNYTTRTAVGHPIGSFYG
                     YEIDGVYKSELEALRDPVSQAIKDKGYFKYKDQNGDKVVDANDKVYLGSAIPWLITGF
                     DFGLNYKKFDVSLSITGQYGNKILNAKRMNRDVFSDGNYDEDFYNNRWTPSNKSTDYP
                     SAEAYNSSFIQQANDFFVENGFYIRIQNVQIGYTTDKIPYVPKFRVFFTAQRPYTFFT
                     YKGFTPEVGGSPISSGIDFNVYPMQAIYTLGIKLIF"
     gene            57024..58511
                     /locus_tag="PK049_00765"
     CDS             57024..58511
                     /locus_tag="PK049_00765"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOK60118.1"
                     /translation="MKTLKYIIITLASVLLLWNCEDFLDFRQEGTLPTTGIDYTKSEN
                     IFLSVSAAYAKLRNWGAHVFPYVGAFEIASDNADKGSTPEDNPEMRDIDNLNFQPNNG
                     LINALWTGYYDIVSGANYAIHQMPLFVQNLQNKSDRDYAIQCQGEAKAIRAYAYFNLT
                     RLFGRVPIVDTVLSSEQLASLSQASTSELYAFIEKDLEEAIAVLPDGYTKEWGGRISK
                     YTAMAIKAKVHLYQSEWDSVASLTDKIIASGRFRLLDNFREVFSMDGENSKESLFEIQ
                     SSTLGKSSGDAAYLEYGYVQGPRGNSPANMQGWGFCTPSQNLIDFYTSRGEIIRPATT
                     LLYRGTKTPEGDSIKMSCANPVYNGKVYTPSSQNRWVYNGYGFDHNIRVIRYSDILLM
                     YAEALVQGATVPLTCGLSADNAINMVRNRAGLPSISGATLQDIWDERRAELAMEEDRY
                     FDLVRTNQAATALASKGFVVGKHEHYPIPAAQLQLNPNLTQNSGY"
     gene            58556..59431
                     /locus_tag="PK049_00770"
     CDS             58556..59431
                     /locus_tag="PK049_00770"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60119.1"
                     /translation="MKNKFRIFGALLFGAVVMGGLLFTACSKDDDNNDNGKVDPSTIA
                     TANLVAYMPFDDNALDKVGNLTPSQQPNVTYVVGRRGKAYQGADGAYLRYNLPDNSKL
                     KTLKSYTVAMWIKSPKVDGTPVPKVFEIGKSDDLFWGNLTIMIERLTAPGADSLFFKL
                     HFKKVGVPWEGQWIEAKKSIFQVNKWMHLVFQYDATTSKFMIYKDGAKVEFPAAVENR
                     YANDQQEPLGDLNFVNADVLNIGAWRPKTDSQATDEWMGWFMGNLDELRVYDKALSAT
                     EVKALYDAEVSQITE"
     gene            59483..61132
                     /locus_tag="PK049_00775"
     CDS             59483..61132
                     /locus_tag="PK049_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HOK60120.1"
                     /translation="MKYVFSLIALTGILITVTFCKEEETVKPIGNIMVDSITIDGIKV
                     YDNGQINHIGFKPNIKVYFDKPVDTTKIDTYNIYFSGDTYNNYTHQFNTDSKVLSIIP
                     NENLKPLTLYRFTVSQGKNLGGNIINGYTAKFFTKLDSTDKFQQISDDSLLTLIQHRT
                     FKYFWDFAHPTSGLARERNTSGDIVTIGGSGFGIMAIIVGIERGFITRAEGISRFSTI
                     VNFLKDADRFHGAWGHWYNGVTGKVYPFSTNDNGGDLVETAFLVQGLITLRQYLNPNE
                     SSENNLINQINELINGVEWTWYTKDGEDVLYWHWSPTVGWAINMQIRGYNEALIVYVL
                     AASSTTYPINPSVYHSGWARNGAITNGKTFYGISLPVGFDYGGPLFFAHYSFMGLDPR
                     NLSDNYANYWQQNVNHTLINRQHCIVNPGRYPLYSADCWGLTASDDNTGYGVHEPTRD
                     IGVISPTAALSSMPYTPEESKKVARFLYYKLGNKVWGEYGFYDAFNVTANWWGTSYLA
                     IDQGPIVVMIENYRSGLCWNLFMSAPEVKQGLSRLGFNTSR"
     gene            61151..62554
                     /locus_tag="PK049_00780"
     CDS             61151..62554
                     /locus_tag="PK049_00780"
                     /inference="COORDINATES: protein motif:HMM:NF021596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HOK60121.1"
                     /translation="MAINGKIGMFWKVSMVLTFLMLSCSKTNNISFPSKGKINYSLTA
                     EEEKMLDSIQYKTFQYFMNEHHPHWGAVKDRAAKWSPISIAATGFAIPCFAIGAERKW
                     ISRDEAAQITLNILKFFKNSEQSPDPLSTGYKGFYYHFLNMETGKREWKCELSSVDTG
                     LLLMGIIFARNYYNANNSVERQIRQIADFLIKRVDWDFMQMPPQGQYAYTISMGWYPE
                     SSIHNMGWKGYNEALFLYILAAGSGMKNIEKAYNSWLESYNWYTPYKDFSHVAFPPLF
                     GHQFSHAFIDFRGIQDSYMSKKGIDYFINSRIATYAQREYCIDNPHGWVGYDSLCWGI
                     TACDGPTDKYNFDNKVFLGYAGRGASGRELNYFDDGTIAPYGHLSSLPFAPEIVLPTT
                     KAMLKRYGDKIWGKYGFYDSFNPTAKWVDNDFIGIDQGPMLIMIENFRTGMVWNYIMP
                     DPIIQSGLKKLGFQNIN"
     gene            62609..65800
                     /locus_tag="PK049_00785"
     CDS             62609..65800
                     /locus_tag="PK049_00785"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HOK60122.1"
                     /translation="MNHHLKATITAALLSVLTFHIGVKAQTVTLDQFESTEGWNYIKS
                     DGVNITAQTDNGLSGKSICITYNFVKGTGYCGIQKLFPINLPENYEITFYIKAESPSN
                     NFEIKFIDSTGNNVWWVNNRNYNFPTSWQKVRVKPRHISFAWGPTTDTRFRRIDRIEF
                     TVASFVGGSGKIWIDSLTFKALPPISNEYPAPVAYEISGTQSKPIAEILDQQPHSVWI
                     AKNKSIQEILIDFKALREIGGLKIDWMQGKQAQKFDVLLSYDSINWEKTFTVNLNINS
                     TSYIRLPEFETRFLKLKLSQSNNSKGLGIKDIEIIKVEESNTLNNFFRYIAKNSPKGY
                     YPRYFLDQASYWTISGVSGDTKEALINEDGMVEVEKASFSIEPVVKVGNKIYDWSNVK
                     TSQSLTYFIDGKTYDYTPTVNWHTDDIDLQVGITSYGNANNSSKLLIGYTLKNNTNKN
                     QTIDFYLLIRPFQVNPYYQTLNLAGGVGTIKKISLDASKDRVEVDNTSLYFTQKFESF
                     FAGSFYSANPTELIRSNHKEVVMAETDNLGLNGGFVKYTIKLKAGQSHTLFAIAPYHS
                     KNINDSELKTKNIVDTIAKVSDYWRGKTEYVKFNLPSTAEHLLKTYRANLMYILINRD
                     IHGIQPGSRSYERSWMRDGSLTSSALLKSGITDEVKEFIEWYAQNLYENGKVPCVVDF
                     RGPDPVAEHDSHGQFIYLIHEYFSFTHDTAFLRKMNPYILRTIGYIESLIAERSTDYF
                     RYGNDSLRAFYGLVPESISHEGYSAKPMHSYWDNFFILKGLKDACDIQIALGNHEKYT
                     RIKQVTDTFRKNLYKSINLTIKNHNINYIPGCAELGDFDATSTTIALTPCNEFENLPK
                     PEIYNTFERYYTYFSQRKTGQINWVNFTPYENRIIGSFIMLNQPERAHELIDYFLSQQ
                     RPQGWYHWAEVVWNDYRTPAYIGDMPHTWVGSDFINAFRNIFVYEDIDHNTLCIGSAL
                     KQDWIDSPQGISVQNLPTYYGNLSYSITKGNDHYKIKIGGNIRVPANGICIRNFNGSK
                     LPKGVEVNGTKLNTYNVNEIRVNEIPCEIKIYY"
     gene            65813..67816
                     /locus_tag="PK049_00790"
     CDS             65813..67816
                     /locus_tag="PK049_00790"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOK60123.1"
                     /translation="MENNNLQAKSEYRIKISQLLAYGAGGIIPIALFNIAGILVGLMG
                     NISLGLSAFWLGTILIIPRLWDAIADPIIGHLSDNTRTRWGRRRPFLLVGGILVALFF
                     VAMWWIPKGETIRIWFPSESGYQWFQLSYILVTLLLFYTSCAIFEIPHGALGMEMTVD
                     PHERTRLFSAKSFVGNLFAMSTPWLFVLANLETFKGPGGNEADGMRYVSLMIAALLIP
                     LSFWWTAKLREPSFVRVQKQEKKPFWTDMKHTTKNRNFIRLTLAIFTLATGFNFVTLL
                     GSYIPIFYVFSGDKVAGAYLLGINGTIWAVTGLLAVFPLNWISPKLGKRNTLILSIAL
                     MATAQLSKIVCYNPEYPYLIIIPTILLSAGMLFFFTLGSSMVGDICDEDDLNTGHRKE
                     GSFYSVFWWFIKMGFAVASFVAGILIEFTQFDQNQVTKVDYLEGSIKEIKVNLTDKNV
                     LNYTDYIKKIKDINTDILGNAIKYDAFLERVNQHENPQTDSLKIQAKVALRQNLSITI
                     DSLKYWDSVLNTSSPDSVNLSIIAQKLNKQRMNFNLIKAQNNVYDLMNHFESIIAGKG
                     DGNHYKELLRDIAQINCVLQEYNIYDIDSVNIHKLDEITQKIPSLKRQSPFTLLMMRV
                     VEIGLPVIMSILSLIFIFRYNLTEKRLQEIKQTLTTRNKLQNQ"
     gene            67831..70269
                     /locus_tag="PK049_00795"
     CDS             67831..70269
                     /locus_tag="PK049_00795"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOK60124.1"
                     /translation="MNKFGIEGGYFSLNGRKVFLNSGEIHYFRIKRELWEEHLKKAKE
                     AGLKTISSYVPWVWHEVNEGEFDFSGTTQPERDLIGWIELCQKHGFTCIVKPGPFILA
                     EYRGAGLPDWFLERYGDTIKMHNSKGEAVLSDGVSLFSPAFLSKVEQWYNHVMPVICQ
                     KQIQNGGPIIMVQVCNEIGVFSWLAHQADYNEQVKEQFISFIKTRYSTIDELNTLWET
                     NYTSFQEIELPPDGNTPYKSKGDRSRDYEWHCFWRKYYANYLRLLSSWIRKHNISIPL
                     YHNLPGWIYGHGYEFPLNITMYEDLYKDKSELIFGVDHIPEFVSYRNLHDDRIINDIV
                     SAMQGEKPLFAAEFQSGSREYHVVTNPREMELFYKASIANGLKGWNYYMFSQGKNPPA
                     KGYSGDTFYWFTPLDNQAKPSSAFPVVQKMSRFIETHEELILTTKRKAEVCVLFYSPY
                     YATELERPEVGASELKFVPARIRRSAYFDGLLKALQVLNVDYDMVDLASSSVNDLLAY
                     KQVWLFSTDEMNAPDQQTIASFVTNGGNLVIFPYLPDREMNQKPCTVLRDTFKIKSSQ
                     PEIIDSPLIKLYNLTDIKCSNPLIAYTTEAIDGATPIAFTLRGSICGFEKNVGKGKVV
                     HLGTWLGFDTEGHLQAYTTILERSKAQLANASSTNNFITVRQKFNDTSESLLFVGNYY
                     NENQSGKITYKHPNTNERVQLPLIDGEIEIPPLYAFISPISKEIGKGINILHTTSDVI
                     GINSNKNIITIHLTGDPGLIGEMVLEGINITKIKSAEISEKPIMLIWHNNRIVLNYLH
                     SNKEILLTLKMLDK"
     gene            70266..73604
                     /locus_tag="PK049_00800"
     CDS             70266..73604
                     /locus_tag="PK049_00800"
                     /inference="COORDINATES: protein motif:HMM:NF017930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60125.1"
                     /translation="MKLKNNSGLEFEFHSNGGIKRISSNGIRIGLRDTNPFSMFGTSI
                     FLRERNTPKKYIMLFGTGSNSVFTFEDNCYFAKGKWNGIEYLISLQLHNTDSCWLWSI
                     ELTNGNSSPSNYDIIIIQDVGLKQLSVGPVNEYYVSQYTERLILDDPQYGAVVCCRQN
                     MKEPTGNPWLMAACKNGAASATTDGLYVYGNSYRKTKIPLALELDTLPGECAAESSVI
                     AIQSIPFDFSSGNNQQISFVFRFLPDHPSATSSTDLAILPNLFEQFKSKYPTTSNWEK
                     GKHNLFISSEFFPSQDLSENEINHFYSSEKRFPDFSNGKLLSFFYGKGKHVVLGEKDI
                     LAYRPHGHILQSNINLNQDDNIVSLTTWSFGVFNSHLKQGNTNFNTLLSINTSQFNLI
                     PETGQRIFVEINNRYYQLGVPSAYEMGLNYARWIYKLDSNIFQVTTWASPNTPVVNFD
                     FKVISGHPVNIIITNNLDELNGWQVKSSGFPNGLTFIPSPESLLSKNYPKAQYRLVIN
                     KSDCANLKFDNGNCIDIESSYANSFVVIKSDSTKQLSISFICELQNLFDQSTINNPDQ
                     QFQADLEDALAFREKSGLEITLSGNSDKIEIINEIIPWFESNALIHYLTPYGLEQFGG
                     AAWGTRDVSQGPVEMLLSLGKYNEVKKILQTIFSNQNPDGWWPQWWMFDRYKTIRAHE
                     AHGDIAYWCIIALSNYIINSGDVDILNSPLPYYTENNGDSREVTPLIEHVERLIDRIV
                     KSFIPGTALVPFGGGDWNDSLQPVNSQLAQRLVSSWTVEMNYQAFKLFETVYRLMGLT
                     DKAEALGEISTKIKTDFNKYLVTNGVVAGYGHLENDNTFTLLLHPSDSKTGVSYSILP
                     MNRGILSGIFDLNQAFNHQKIITNHLLGPDGARLMDKPLKYSGGLQKIFQRAESSTFF
                     GREIGLMYTHEHIRYAETLAVTGKAEELIRAILQAVPISYTNIVKQGDIRQSNCYYSS
                     SDVLFKNRYEADKLYPEVIKGNIKLHGGWRVYSSGPGIFIGIILGRLLGFRFSNEFVV
                     IDPVLPKEFDGLRVNFSFSNIPFSVTYHITKSTFSPLSLKVNHVEPPFTYEENPYRKG
                     GIRVERNLFFGLLNSKENRIEIVL"
     gene            74588..74788
                     /locus_tag="PK049_00805"
     CDS             74588..74788
                     /locus_tag="PK049_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60126.1"
                     /translation="MVNFESFSVHEMNTSEMTDINGGFSWEDYLIGKGIDAALECMKE
                     LFIAYVNYSASTGGKYVIHHAQ"
     gene            74841..75365
                     /locus_tag="PK049_00810"
     CDS             74841..75365
                     /locus_tag="PK049_00810"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOK60127.1"
                     /translation="MIKPYKLKYSIQSVNPLLVIIITTITLLLIESFYHYLFLAFDIH
                     HTSNLPKLPVETDPESHVKIAIRLVKVCIIAPLVETFIFQYLLFELFWRKLRWSLNVF
                     YGLSAFTFGLSHFYSIPTIIVTFTVCLVFNYVYAALAEKKLNLNAYFVIVAIHSLYNT
                     SVFVNHIFSYLGSL"
     misc_feature    complement(75676..76303)
                     /note="23S ribosomal RNA rRNA prediction is too short"
BASE COUNT        23194 a        16469 c        15287 g        21505 t
ORIGIN      
        1 ttaaaggata aaggttaaag gataaaggtt aaaggataaa ggttaaagga taaaggataa
       61 agatttaaga tttaagattc aagacttatg attaaaaaaa tgatgtgaag agatgtaaag
      121 gattatagta tttaactttt aacggttcac gtttaacgac tttacagctt cgcccagtca
      181 gtcacaccca agggggatga cctaccagga attattccac gaaaataata taaataaagg
      241 aaacaagacc ataaaaaaag tcactaaata taaacaaagc cccatttagg ggcttaattt
      301 tctgataatc aaaagtaagt tttagaggct ccagtatgtt aaattatgta tcttccccct
      361 gtaaatgcct ttaacatcaa caaaaacaca tgaagggttt gcaaggtttt taaaccattc
      421 ctcgttaagg ttgatgtact ggttatggct aacggcaaca attattgcat cgtacttacc
      481 atttggggct gaagccatat cgaaaccgta ttcttccttc acctcgtggc tgtcggcata
      541 ggggtcaacc acatcgatat tctgaatacc gaacgatttc agctctttgt aaatgtcaac
      601 cacctttgag ttacggatgt cgcttacatt ttccttgaat gtaacaccta aaatgagcac
      661 gcgcgactcc tttgggtttt tatcggcagc aattattttc tttacggtct gctttgccac
      721 gtaaccgccc atggagtcgt taatgaaacg gccagcagta atgatttgag ggtgatagcc
      781 aagctcttta gccttgtaaa ccaagtagta agggtcaaca ccaatacaat gaccacccac
      841 caatcccggg aaaaacttta gaaaattcca ttttgtacca gccgcttcaa gtacttcgta
      901 ggtattaatt cccatacggt taaaaatgat agaaagctca ttcataaagg ctatattgat
      961 atcgcgttgg gtgttctcaa taatctttgc agcctcagca accttaatgg aactggcacg
     1021 gtgaatacct gccttaataa taagctcata ggttttagca atctcctcgg ccgagtcggc
     1081 atcgcaacca gaagttatct ttttaatggt tgtcaaagta tgctccttgt caccggggtt
     1141 aatacgctca ggcgaaaagc caaccttaaa atcctgaatg tactttaaac ccgatagctc
     1201 ctcaagcaag ggtacgcaat cctcctcggt acagcccggg taaacggttg actcgtaaac
     1261 cacgtaatcg ccctttttta gtacttgacc aacagtgcgg gttgctgcaa gaagcggttt
     1321 taaatcgggc aggttgtgtt catcaattgg ggttggaact gcaaccacgt agaacttggc
     1381 cttgcgcaaa tcatcgatat ttgaggtaaa ctcaatatcg caacctttaa atgcttcagg
     1441 ttccagctca cggctggggt caataccttt tttcatcagt tctaccctgt cgggtttaat
     1501 atcgaaaccg ataactgaga gtttgcgggc aaattccaat gctataggta aacccacgta
     1561 acccaaacca ataactgcaa gtttttcctt ttttgaaaca agttcgctat acatttttaa
     1621 aggtttaaag ttttacaaaa tgggtgataa tcgccggtta aacctaccgg tgccatatta
     1681 cggatggtgt aaacagtttc aattgaagca cgggcatcgc ttaacccaaa gctattacct
     1741 gcgagaattt cgcggtagct tgcagtatgc aaatcggtaa acccctcgct gaactccaac
     1801 tcattatcgg caatgcaaat ggaacggtag gttcgctgcc ccttttgctt tatattatca
     1861 ggaatatcgt tatagtcaac acttaggaac cagcgtacgc gagccttttc gagttccatg
     1921 tatccagcag ccttgtcggg gtgggaaaca tgcaccacgc tcgacttaac acttccaaaa
     1981 atccatgaaa gcatatcgaa aaagtgcaca ccaatattgg tggcaacccc acccgatttc
     2041 tgagtatcgc ccttccatga aataaagtac caacgtccac ggcttgtaat gtaggttaaa
     2101 tcgatatcaa aaaccttatc cttaggagcg ctatcgatct gcttttttaa ctcaatgata
     2161 gtagggtgca atctgagctg aagtatagtg ctgatacgtt taccggtttc cttttcaatt
     2221 tcagcaaggg catcaatatt ccaaggattc agcacgattg gtttctcgca aatagcgtta
     2281 gcaccctgtc ttaaagcaaa acgaatatgc gaatcgtgca ggtagtttgg agtgcaaatg
     2341 ctaacgtagt caaccttaac gccctgtcgt ttaagcttat cgatatgcct atcgaatcgt
     2401 tcatattcca caaagaagtc agcctcaggg aagtagctat ccataattcc tacacagtcg
     2461 tgcttatcga gtgctgcaag caaattattg ccggtttcct taattgcttt aaggtgacgg
     2521 ggagcaataa atccggctgc tccaataagt gcaaaattct tcattgccta atctattcga
     2581 aaaacctgat tattctcaag cttatacttt tgtccactct cagggcaaac tgcaatgcca
     2641 tcgctattaa actcaagacg atggccatac tcgctcatcc aacctatttg ccgggctgga
     2701 ttaccaacaa ccagagcgta aggctttacg tccttagtta ccactgctcc ggcaccaaca
     2761 aaggaaaact cgccaagggt aacgccgcat actatggttg catttgctcc aatggtggcg
     2821 ctacgcttta ccagcgttgt taaatactca ctcttgcggt taatggcgct acgcggattt
     2881 attacattgg taaacaccat tgatgggccc aaaaaaacat catcctcgca aattacaccc
     2941 gtataaatgg aaacattgtt ctgaaccttt acatttcggc ctaattttac tcccggggaa
     3001 acaaccacat tttgcccaat attgcaactt tcacctatct cgcagccaga cattatatgg
     3061 ctgaagtgcc atatttttgt acccttgcct attgtgcaac cttcatcgat tactgctgtt
     3121 tcgtgggcga agtaggtttt ctccatgaca ataaaaattt atcaaaatta atcttttaat
     3181 gtctccagta gagtttcggt gatatatttt attacatcgg ctgatagctc ggtatgcatg
     3241 ggaagcgaga ttacagaact tgatagaaaa tcggaaacct gaaacgaacg cccataatta
     3301 aactccctgt agggctcaaa agctttttgc ttatgcaacg gtattgggta gtaaaccatg
     3361 gtaggaatac ctttggcagc caaatctttt tgtagccttt cgcgatcaac attgttaagc
     3421 tttaatgtgt actgatgaaa gatatggcta ctccacttag ctcgtgccgg cgtaataatc
     3481 ttgctacaag gttttaacgc ggaatcgtag gcatttgcag cctcaatacg agccttatta
     3541 taaaaatcga ggtacttcaa cttaaccctt aaaatggcag cctgaatagt atcgagacgc
     3601 gagttaactc ctattcggtc gtggtgataa cgcaccttca tcccatggtt ggcaattgaa
     3661 cgtatctcgt tagccaagct atcatcattg gtaaaaagag cacctccatc gccatagcaa
     3721 cctaagtttt ttgaagggaa aaacgatgtt gtgccaatgg ttcctatggt tcctgctttt
     3781 gaaacccggc cattggagtg gtaataatcg gcacccaatg cctgtgcggt gtcctcaata
     3841 acggataggt tatacttttg agctaaacgc attatctcat ccatgttggc gcattgacca
     3901 aaaagatgaa caggaataat ggctttggtt tttggggtta cggcttcctc taacctttta
     3961 gtatcgatag taaaggttga tggatcaaca tcgacaagaa ttggggtaag gccaagaagt
     4021 gcaactactt caaccgtggc aataaaagta aagtcgggcg tgataacttc atcgcccggt
     4081 tttaaatcga gtgccattag tgctatttga agggcatcgg ttccgttggc acagccaatt
     4141 acatgcttaa caccaaggtg cgaagccaat tcttcctgaa acaaactaac ctctttccct
     4201 ttaatgaaag ctgtactgtc gataacttcc tgaatggcag catcaatctc actttttatc
     4261 tttaagtact gcccccttag gtcgcacatc tggatgttca tagatttaag gttaaaggtt
     4321 caaagttcaa ggataaagga taaaggataa aggataaaag ttaaaggttc acatttaata
     4381 ctgtacactg cacactttac actaaacact acttagcgta gctgatggct cgggtttcgc
     4441 gaatcaccgt aattttaacc tgacctggat atgtcatctc gtcctgaatc tttttggcaa
     4501 tctcgaacga gagcttattg gcttcttcgt ccgacacctt ttcgctacca acaattacac
     4561 gcaactccct accagcctga attgcatatg ttttaagcac tccggggtat gaaagcgcaa
     4621 ggtcttccat ctccttaagt cgcttaatat acgattcaac aacctcgcga cgtgcacctg
     4681 ggcgtgcacc tgaaatggcg tcgcaaacct gaacaatagg agcaatgagg gtggtcatct
     4741 ccacctcatc gtggtgcgag ccaatagcat tacaaacctc gggtttctcc ttgtacttct
     4801 cggcgagttt cataccaaga attgcgtgtg gtagttcggg ttcatcatcg ggtaccttgc
     4861 ctatatcgtg cagtaagcca gcacgcttgg caaccttggg gttaagccca agctctgaag
     4921 ccataattgc acaaagattt gcaacctcac gcgagtgctg gagcaggttt tgcccatagg
     4981 atgagcgata cttcatttta ccaaccaggc ggatgagctc aggatgtaag ccatggatac
     5041 ccaaatcgat agcggtgcgt ttgcccacct caataatttc ctcctcaacc tgtttttgaa
     5101 ccttttgcac tacctcctcg atacgcgcgg gatggatgcg gccgtcggtt accagctgat
     5161 gaagggcaag tcgggcaatt tcacgacgca cagggtcgaa agctgaaagg atgattgctt
     5221 caggggtatc gtccacaatg atttccacac cggtagcagc ctccagggct ctaatgttac
     5281 ggccttcgcg gccaatgatg cgacccttga tttcatcgga atcgatattg aacacagtaa
     5341 ctgagttctc aatggccgtt tcggttgcaa cgcgctgaat gctttgtacc acaattcgtt
     5401 ttgcctcctt ggtggcagtc attttggcct catcaacaat ctcgttgatg tacgacatgg
     5461 cctgggtgcg agccttggct ttaagcgact caataagctg agctttggcc tcctcggccg
     5521 aaaggcccga aattgcttca aggcgctcaa tttgatcctt atgcatacgc tcaagctcct
     5581 cgccacgttt ttcaaccagc tccatttggc tcgagaggtt ttcgcgaata acatcaacct
     5641 ccttctgctt acgctgaagc tcctcaaact tttgcgataa ctgcagttcg cgctgcttga
     5701 ttttggtttc agcttgagca atccggttgt ttttttccga aattacctta tcgtgttcag
     5761 ttttaagctg taagaacttt tcctttgcct gcaaaatctt ctccttacgg ataacttcgg
     5821 cctcggcttc ggcctcctga ataatcttat tgcgcttgct ggccaaaagc ttattccagc
     5881 taaagtacga cagtaatccc cctagggaaa aagccgaaac tgctattatt attgttaaca
     5941 ccatactaaa tgaattttta agaattatat atataaaaaa cccgcattat gccttaattt
     6001 tatgtagaaa ccccagctaa acatgggtgg agaggccatc cgattcagac taccagcgtc
     6061 tcccggagga gatcccgaac aagcggtaaa caggcctgtc ttgtgcggag aaagtccttc
     6121 tccaattttt gtagtgttga gtttcgcaaa aacgttaagg cgataatgcg ggcaatgcta
     6181 tttgaaagaa ctcatgtttt gcttaccaat gaaagaaccc agccactcgt ccagatcttt
     6241 caactcgctc ataaactccg aggtgtcagt tttatcctcg cactctatca gctttataac
     6301 gaactgtagt gctgccatag ccaaataatc ctgtacgtcc ttgtcgctat acctttgctt
     6361 gtacagcatt accttgtcgt ttatcaattt agcagcctta cgtatctttt cctcatcgtc
     6421 tctgtcgatt ttgaggggat ataaccgttc ggccacattc acgttaattg ataacttttc
     6481 gtccattcgg cagcgctagg ttctatttat taagtagagc aatacacctg tcaatttccc
     6541 gcacaatccg gttcacctta atctttgcct cgtgcacatc ggcatcgctt gccttcagcg
     6601 ccttggctat ctttaaccgg gaatatcgtt cttcaagctc ggcgcaggct tgctctttag
     6661 ctttcagttt agcttcaagc tgagcaacct gagcctttaa acggtcgttc tcctgttttt
     6721 ctctttcata cataaaaaca agagtttgaa ccttttgttt aatgttgtcg attatatggc
     6781 tcgaagaatc gttcatatcc cagataaagt tttacaaagt taattaaacc tacttcaaaa
     6841 aacaaatctt taaacaattt ttaaactcag cagagtgggg ttttccataa atctaataat
     6901 gaaatgctta cgctcaaaac taaaatattt ctatcggttc atttcaattt taagcaccac
     6961 tgaaggtagg cttttatata tttgggcata aaaagataat aacaatgagg cttttccgct
     7021 ttgcaggttt gctatttctt tcaagtgtta caattgctca aacacaaccg tttaagttac
     7081 cactcgaaat gccatccgcc ttttcgggca catttggtga agttcgatcg gatcatttcc
     7141 actcaggggt cgatcttcgt accatggggc aggtaggtta tagcgttttt gccattgaaa
     7201 aaggttttgt ttcacgaatc aaggtaagcc ctgttggttt tggtaaagta atatacatcg
     7261 atcaccccaa tggcaaaaca tcggtatatg ctcacctcga taggtttgtg ggtaaaattg
     7321 cagagtatgt tgagaatgcc caataccaaa aacagtcgtt cgagattgag ctcttcccta
     7381 acccaggaga actaaatgtg aataagggcg atattattgg gctatcgggc aacagtggta
     7441 gttccggggg gccacacctg cacttcgaga ttcgtgaaac cacctcgcag catattttag
     7501 atccgtttga gttttttaat gaatggaggc aaaccgatta caatccacca accattaacc
     7561 gtttgcttgt ttttgaaatt gacagcttaa gatacctgct cgacagctta acctataaag
     7621 cctatgaagc cgtaaaaaag gggagcgatt ttattgttcc attgccaatt aaagcatcgg
     7681 gtaaagttgg cctagcactc gattactacg atattgtaaa ttcaaacagc ctgagagccg
     7741 gtatcaagaa agccgaagtg agaataaatg gtaataaaac ttttaccttc cgtgttgacc
     7801 gattctcttt tctggaaacc cggtatgcaa atggttttaa tggtattacc gataatggat
     7861 ggaatggcta taaagcaatt cggctatgga ccgatcctaa caacaggttt tcgggcatca
     7921 atcatgttat caaccatggt attattgata tagctaaaga tagttcttac actattgatg
     7981 ttacggtttg ggatcattca ggaaacagta gcaaactaat attcaagata ttaggcgtgg
     8041 aaaataaggt aaacgctaca agcgtacctt cccttcctct tttcagatgg aatgctgaaa
     8101 atacatacaa aaccgatagt tacatggtta ggttgcccga atatgctcta ttccacgata
     8161 tactattcca aatggaagag aaccagtccg ataatctacc atacccaaca gtcactattc
     8221 ataatccaaa aacaaagctc ttcaaaaagt tcacattgga atacaaagcc agcaaaatac
     8281 ctgaaaacat tttgagtaaa cactacattg caaaattaaa cgggaaagga tttgaatacg
     8341 ttggaagcga acttattaat ggtagcctaa aggcaacctg caattgcttt gggaaatata
     8401 ccgttctggt tgatacaatt cctccgtcaa ttatcgccca aaacttaatg cctagtgcaa
     8461 aaatcaccag tcaaaacaac ataaacttta agctaataga caatacgggg atcaaaacgt
     8521 ataatggcta tattgatggt aactgggtgt tatttgagtg ggatccaaaa accggtatac
     8581 tttcgcataa tcttaatccc caaaggatta aaaaaggtca atggcatagc cttaaaattg
     8641 ttgctaccga tatgttaaac aatgattcgg aatatgctat caagtttttc tggtaatagc
     8701 taactttgac atagtaaact gattgagttt tgataaccgt tttttacttc atactattcc
     8761 tcatctcaat agtcattgta gccataattc catggggctt aatctacggc tacagtaatc
     8821 ttatggctaa actcctgcac aatgttttaa aataccgtgt aagggttacc cgaagcaatt
     8881 tacgattggc attccctgaa aaaagcgatg cggaattagc agtaattgag aaacaatcgt
     8941 accagaacct agccgatatt tctgttgagg cgttaaaagg gttcaccatg cgcaactcag
     9001 ccataagaga acgtcataaa attcttaacc ctgaactact cgattactac tacaatcaga
     9061 aacagagtat tattggagtt gcagggcacc taaacaactg ggaatgggga gccctctcgg
     9121 ccggtataca gctaaaacat cacccggttg ccatatacaa accgctaagc aacaagctta
     9181 tcgattggtt catcaagtgg aaccgatcga ttaggggaac cgaattggtt ccaaccaatc
     9241 agacctttga tacctttcag aaatataaac acaaaccttg catatacatt ctggttgctg
     9301 accaaagccc gtcaaaccta tcaaaagcat actggataaa ctttttcaac caggataccg
     9361 cctgcattca tggtcccgaa aagtatgcaa aaatgtataa ttaccctgtt atatacatcg
     9421 atataaaaag ggtaaagagg ggttactata ctgtagaact aagcgttttg tgcgataaac
     9481 caaacgaact gaaggacggt gagttaaccg ctgcctacat gcacaggctt gaacaaagta
     9541 taagagaaaa ccctgaaagc tggctatgga cgcataggcg atggaaaaga aaaaggccac
     9601 aaaaggcaca atagaactaa tttggttcac tttttgataa gttttattac atttgaaaaa
     9661 aattcaagcc atgaaaagat atacttcagt tctgctggca ataactttat ttttctttag
     9721 cactcaggtt tttgcccaaa aatacagaaa taaagaggta gggctgattt atttcaaggt
     9781 caatattgat cccggatacc atgcaaagtt tagcgagtat gctcaccttt tccgcgagcc
     9841 ggttaaacca cagcaggata gaattgttgg acaccttact gccgatatct gggatattat
     9901 taagcaacag cttaccaacg atgttggtat gattatattg cccctggatg cctacggcga
     9961 taaattttcg tacgatcact atggatttcc aaatgtgaca ataaacagag ctcttcagaa
    10021 agggaactca aagttttata tcagcattga tgtggaaatt ggccctgagt ttatgtcgaa
    10081 atatgtaacc aaaacatcca gcgacacctc accacaggta atccaactgg ctcccaatga
    10141 gataaagcca aagattacca ttaaaatgaa catctataac gataaaggaa taataccagt
    10201 agccaacact aaaggtgaag ctattgaaag taaagtaata acgctgaacg ataagtattt
    10261 tgacggtttt gtaaacccaa acataaactc accaaacgaa accctttata aactggtcaa
    10321 ccaagctgtg aaggaactag taataagcgt gtattcgcgt aagtaatgca ttccccaaac
    10381 aagtcttcag gttataggat gataggctta atgtcgggca catcgctcga tgggctcgat
    10441 atttgttact gcttttttca gaacataaat agcaagtggc aatactacat tttagctgcc
    10501 gaaaccatta agtatccaac acatctcata aatcagctaa aggaagccca taattacact
    10561 gctgaggaga ttaccgcttt agactttagc tacgggaagt atttaggtga acaggttaaa
    10621 cggtttgtat cggaacatgg aattgaggcc gattacattg catcgcatgg acacaccgtt
    10681 tttcataatc cgactcaagg atacacccta caaattggca aaggtgcagc aattgctgcg
    10741 tattcaggaa ttccttgcat ttccgatttt cgttcgggcg atgtttcacg gggggggcaa
    10801 ggtgcaccgc tagttccaat tggagacaaa atgctttttc aagagtttga catctgcctt
    10861 aatttgggag gcatagcaaa tattagctac gatgataatt ttagcaaccg ggtggcttac
    10921 gatatttgtg tttgcaacat gatgcttaac cacctagcag ggttaacagg taatgaatac
    10981 gattttaatg gtgaccttgg aagaaccggt caggttattc cgggaatgct ccaggaactt
    11041 gaaaatattg aatactacat tcagccaccg ccaaaatcgt tgggtaggga gtggttcgag
    11101 agctatataa tgcctatact caacaggtat aatgcatatc cgcttaccag tcagttaaga
    11161 accgcatacg agcatatttc aggtagaata gcccacgatt gtaacagctt gaaaaaaaca
    11221 accatgctgg caaccggtgg aggtgctaag aatgcttttt taatggaact aatcaagcag
    11281 aaaaccgagt tgaaaattgt aattcccgat agtaaaacta ttgattataa ggaagccctg
    11341 atatttgcat tccttggggt gctttactta ctaaaggaac ccggcgccat agcctcagta
    11401 acaggagcaa acaacaactc catagccggg tgtctataca gctaaaaccc cgaaccatta
    11461 aaccatcaac ctggtttata atcaaaaaaa tattaggttt gtaaacttta attaaactaa
    11521 aacgattatg agaaaactca caatccagat ttttgctgct attgcattac tgattaatgc
    11581 caataccggc aatgcttgca ccaattacct tgtaaccaaa ggtgcatcgg ttgacggctc
    11641 aaccatgata acctacgccg ccgactcaca ttacctgttt ggcgaacttt atttcaaacc
    11701 agctgctgtt tatccggctg gtacgctaat gaagatttac gaatgggata cagggaaatt
    11761 ccttggcgaa attccccaga ccttacagac atatagcgtg gtaggcaaca tgaacgaata
    11821 tcaggtagca attggtgaaa ccacctatgg cggacgtgag gagctacagg atagtactgg
    11881 gttaattgac tacggttcat taatgtatat cgccctacag cgctcaaaga gcgcacgtga
    11941 ggccattaaa gtgatggctg aactggttga aaagtatggc tactacagtt ccggggaatc
    12001 gttctcggtt gccgacaaga acgaggtttg gatttttgaa atgattggaa agggaaccga
    12061 tattaaggtg gacaagaaaa ctaaaaaggc ttacaacgca aatcggggag cagtatgggt
    12121 agccattcgt attcccgatg ggtacgtatc gggtcatgct aatcatgctc gtatcaccac
    12181 cttccccctt gagaacatga aaacctcaat cagctcaaaa aatttagata aaatttttga
    12241 acccgatgtt gaggttgttt acgctcatga tgtaatcggc tttgctcgtc aaaaaggtta
    12301 ctttaccggt aaagatgagg aatttagctt ttccgatgta tatgccccac tcgattttgg
    12361 tgcagctcgt ttctgtgaga ttagggtttg gtcgtttttc aaggctgtaa acaaggatat
    12421 gtggaactac tttgactacg ccaaggggca cgaccttaaa aaccgcatgc ctttatatat
    12481 caagcctgag cgtaaactct cggttaggga tatgatgaac tttatgcgcg atcaccttga
    12541 gggaacagaa ctcgacatga gtaaggatcc aggcgcaggt cctcatggtc tcccataccg
    12601 ttggcgtcca ctaacctgga aatacgaagg gaaatcgtac tttaacgaac gcgccactgc
    12661 aacccagcaa accggattct cgtttgttgc acaaatgcgc agctggctac ccgatgctgt
    12721 tggcggtatt ttctggttca gtgttgacga tgctgcctca accgtttact tccctgtata
    12781 ttgcagcgtt acctctgtgc cagaggctta cgccgagggc aagggcgata tgctcaccta
    12841 ctgcgacgat tgtgcattct gggtattcaa taaggtatcg aactttgctt acctccgcta
    12901 cgaccttatg attcaggata tcaaaagggt acaggatgag ttggaaaacc gtttcatctc
    12961 acaaacagcc gtagttgacc aaaccgctct taagctattg gaaaccaaca aggaggatgc
    13021 tgtacgattc cttaccgatt ataccgttaa taccggaaac tatgttgttg ctcgctggga
    13081 aaaactattc cagttccttt taatgaaatt tatggatgga aatgttaagc aggaggatgg
    13141 aaagggtggg cttaaataca accagtacga ttactgccct gccccagtta aaaatccaca
    13201 ataccccgac tggtggaaga aaaacgtgat tgatgctacc ggcgataagc tagctgttcc
    13261 tgaaggtgct gctagtcact aaggcatcaa gcttaaagtg ataaaaaaga gcaatcgtta
    13321 tccgattgct cttttttctt tagccaaccc attttatggg tttatgtaca aaaaaacagc
    13381 aataccctaa ataaatttta tgaccataca gcaaatgata catataaatc gtatttttag
    13441 attgttgaaa accaaattca aatcaaatgg aaaaaattga gaacagcccg aaaaccgact
    13501 actcaagact agtacttgga tttttgctaa ttgtgttggc aggtttactc cttatcgata
    13561 acttcgattt gtatcctgtt gaatggggca aatacatttt cacctggcaa acgttactaa
    13621 ttgtaatagg attaattatt ctgcccggta aagggaatag aactacagga actgttttaa
    13681 ttgccatagg ggcatttttc ctggctgcaa agttttacga attgcccgtt tcaattagta
    13741 aacttttttg gcctgcagta attcttttta taggcctttc attaatattt ggtagcaggc
    13801 gacgcaggtg gcacccatac aaaacaggtg aagttaagga cgacttgatt gatgatgttt
    13861 ccatttttgg tggttcggac caaaaattta cttcgcagca attcagaggg ggaaaaatta
    13921 ccaacatttt tggaggctca accattgacc ttagcagcgc aaagctggaa tcaggcttaa
    13981 atgttgttga tttgctatgc atttttggtg gctcaaaact cattattcct tccgaatgga
    14041 aagtaaaaat tgaggtaacc tcaatttttg gaggcgtttc cgataaacga aaaatacctc
    14101 aggctctacc agaaaatgcc ccagagcttg ttcttaaagg attagtgctt ttcggtggag
    14161 tagatatcaa aagttactaa gatgtaataa tagttgaaaa ggctgacagc aacctgtcgg
    14221 cctttttttg tcaaaaaaag tagctctcta acacattgta caggcttgtt acttttacat
    14281 aattcattta cgtttgtgta aattgtaaac tgtaaaactc aaaacgcatt aaaatgatta
    14341 ccaacgagtt aatcaatccc aaaagtatag tggtggttgg cggttctgac gatattcaga
    14401 aacccggcgg aaaggtttta aaaaatctta tcgacaacca ttttaaaggg agcctttacg
    14461 tagtaaaccc aaaagccgat gaggttcagg gagtgaaatc ggttcgcgat gttgctgatt
    14521 taccacaggt ggatttggca atactagcca ttgcagctaa gttctgccct caaaccgttg
    14581 aggttctggc cacacaaaaa ggaaccagag ctttcattat tctctcggcc ggttttcatg
    14641 aagagagtga ggaaggcgca aaacttgaac aacaaatagt tgacaccata agccgtaccg
    14701 gtgggtgcct aattggccca aactgcattg gtgtaatgaa cactaactat gccggcgttt
    14761 ttaccacccc aattcccagg ttcgacccca aaggagttga tttcatttcg ggttcaggag
    14821 ccactgcggt gtttatcatg gaatcgagca ttccgcgtgg cttaacattt aacagcgtat
    14881 actcagtggg caatagcgcc caaattggcg ttgaggaagt acttgaatac ctcgataata
    14941 cattcgaccc gcaaagcagc tcaagggtaa agctgctcta cgttgaaagt atcaacaaac
    15001 ctctaaaact cttaaaacat gcccagtcgc tcataaggaa agggtgcagg attgcggcaa
    15061 ttaaggctgg cagttcatcg gcaggaagcc gagcagcatc gagccacacc ggagcgcttg
    15121 ccagtagcga tgtagctgtt gaagcgctgt tccgcaaagc gggaattgtt cgctgctatg
    15181 ggcgcgatga gctggctacc gtggcatcaa tttttatgca tcccgagctt caagggaaaa
    15241 acattgcagt aatcacccat gcaggtggcc ctgctgttat gctcactgac tctttatcga
    15301 acaacggact tgaagtacct ccaattgagg ggccaaaggc aaaggaactg cttgccaagc
    15361 tatttccggg ctcatcggtg gctaacccca ttgacttttt ggcaacagga acagccgagc
    15421 agcttggatt tatcattgat gcctgcgaaa acgacttcca taatatcgat gccatgatag
    15481 ttatttttgg aagtcccggc ctattccccg tttacgatgt atacgacctg cttgatgaaa
    15541 agatgaaggt ttgtcgcaaa cccatattcc ctgttctccc ctcggttatg aacgtaaaga
    15601 atgagattga acactttatt gctaaaggac gaattttctt ccccgatgag gtatcgcttg
    15661 gcaacgccct tgcaaaaatt taccatacac caaaaccatc acctgaagca cccgttttac
    15721 caaaggtaaa tgaaaaagcc ataagtgaga ttattagtaa aagcaataac ggttacctga
    15781 cacccgatgc tgttcaacag ctgctcgatg ctgccggtat accccgtgct ggcgaagcgg
    15841 ttgttacaac tgccgatgat gctgccagag cagcagagaa attaggctat ccggtagtaa
    15901 tgaaagtagt tggccccgtc cacaaatcgg atgttggggg tgttgtactt aatgtgaaag
    15961 attccgaaac ggttcgcaaa gagtttgacc gtatgattaa gattaaggat actacagcca
    16021 tccttattca gccaatgctt aaaggggttg aactttttgt tggggcaaaa cgcgaggata
    16081 aattcggtca catggtacta tgcggacttg gaggtatttt tattgaggtt ctaaaggatg
    16141 taaaagctgc ccttgcacct attgatacaa acgaggcatt aaacatgata aaagggctta
    16201 aaagctatgg aataatcaag ggagcaagag ggcaggaacc tgttaatgaa cagatttttg
    16261 ccgaaattgt tagccgttta tcggcactcg taacagttgc tcctgaaatc tttgagatgg
    16321 acctaaaccc gcttctgggt tcaaaagata aggttgttgc ggtagatgcc cgaatcagaa
    16381 ttgaaaaata gtgcgccatg agccttaagg agaaatggaa tagccttaca gctaacgaaa
    16441 aaatgatgat tggccttgcc attattcttg taatacttat tgcaacacgc tttgagtata
    16501 tatgggatca ggttaaggct ggttttgcta gatattttaa ccgttaaatg ttgattaaag
    16561 tgaaacgagt tgacttgtac gtaatggtag gaactatttt gttccttcta ccctttttta
    16621 tatcggaaac ggtttacaac ttttatgttg attttaacgc cagccatgga atggttacaa
    16681 gtttcataaa gtttgccctc cttgccacct tcggcgaagc cattgggtta aggattagaa
    16741 caggaaacta taatcaaccc ggatttggat tgttgcccag agcggttgtt tggggcttgc
    16801 ttggtttaac cataaaatta gcttttgtgc tatttgcatc gggggttcca caatttcttg
    16861 catatatggg catggaaaat gcgccccagg ccttaaaagg cgaacttact ttagccaaaa
    16921 tactcgtagc cttcagcatt agcctgttta tgaacattat ttacgctcct gtaatgatga
    16981 cactccataa gattactgac acccacatag tgaacaatgg tgggacactt tcgtgccttt
    17041 taaagcccat acattttggt aatattcttg catcgataaa ttgggaggtt caatggagtt
    17101 ttgtttttgc caaaacaata ccctacttct ggatcccagc tcataccatt acatttcttt
    17161 tgcctgccga ttttcaggtt ctttttgcag ctgtactgag cgtggcgcta ggaatatttc
    17221 ttgctgtggc aagcctgaaa ggtagtggta aatagaaatc gtagaaaaat tatatattta
    17281 catcaaaagt aaagaatgaa atatgaataa acgtattgga atattttacg gacccaccgg
    17341 aggttctact gagaaggtgg ctcagctgat agccaaagaa cttggagcga tagctgactt
    17401 acaccccata aaagaatcaa cgccttcaat ggtaaacaat tacgacatta tcgtttttgg
    17461 atgctcaacc attggtagcg aaacatggaa tggtgaggct tcgcgttcgg attgggacca
    17521 tttcagacca gagctgaaaa aaatccaagt tcagggcaag ctctttgcct tttttggaac
    17581 cggcgattca gttacctacc cccgcaattt tgtcgacgga atgggaattc ttgccaagga
    17641 attaattgct caaggggctc aggtgatagg ccaatggcct accgatggct acagttttac
    17701 cgactccgaa gctctaatta atggaaaatt cattggctta cccattgatg aggattacga
    17761 ggccgacaaa actattgccc gcgtaaaagg ttgggttgaa ctaataaaga aatcaatcta
    17821 ggtattaggc attctaaaaa gaaagagcca caatctgtgg ctctttcttt tctcccttct
    17881 acggcagtga attcctaaaa ctttggtatc ggtcagcata ggtattaccg accttactgc
    17941 ctaaacttct ccacacatca tcaatgttct taatcctatt ctcatccgat ggatgagtgc
    18001 taaggaattc agggattttt tgtgtagaac ccatcatctt ctcaaaaaag ccagcaagtt
    18061 cgcgtgggtg ataatcggta tcgcaaaggt aagtaacagc atacttatcg gcctcatact
    18121 cgttatcgcg actgtatttg agcattgttc caccaagggc taactgtgat agtatctgct
    18181 caagctgtga gggattatcg cccataataa ttgagagcaa catgctaaaa ccatactgac
    18241 gggtaagcat ttgagtgctg tgacgcctat cggcatgggc tatttcatgc gccataacgc
    18301 cggctagctg agcttcgtta tccagaaact taattaatcc ggtgtaaaaa tataggtaac
    18361 caccaggtgc cgcaaaagcg tttaacacat tgtcgtcatg gatgattttt acctgccagg
    18421 gaaatgtagt tgtatacctc agatcatccg aacttaaaat ggcattcatt accctctcaa
    18481 tatgtgtata tgcctcagcg tattgagtcc tgtcaagaat aggatattca ttggggtttg
    18541 ccaaaattgc ggaatcgagc tgcgcaccaa attgtatatc ctgttcgaga gtaaaaaagt
    18601 ttatgccatc gtcggaacag ctgcttagca gtatcacact cgttaaaacc caaaataatc
    18661 tcttcatttt atcacttttt atcttgattt aatttattga atttcaactc aatacacaat
    18721 tcaatgcaaa tcaaatcttt tttgtcaatg caaatttaat atattagcaa acattatcga
    18781 taaaataaat tattgattgg tagagcaaca agtcaacaat tgatgacgga agcaggaaac
    18841 attgcaaaat taagttttaa gaaattgtta gtaagagtaa taacctactc ggtagtacta
    18901 ttctttacac ttagtattag tttgactgta gtgtacaaat gggttaaccc acattacaca
    18961 tggttaatga tactaaaaaa gcatttaccg gaaacccaag gaggaagtag caattttgaa
    19021 cagagttggg taagtatcga agagatatcg ccacagatgg tacttgctgt aattgcagcc
    19081 gaagacaata agttcatgga acacaacgga tttgactggg aatcgataaa aaaggcccga
    19141 aagtataatc aaagaggtaa aaagttgcgc ggagccagta caatttcgca gcaaaccgca
    19201 aaaaatgtat ttctttggcc caaacgttcc tgggtacgaa aaggattaga agcctacttt
    19261 accctgctca ttgaaacttt ctggtcaaag gaaaggataa tggaagttta cctgaacgta
    19321 gccgagatgg gaaggggtat ttacggagtg gaagccgcag caaggaaata ctacggtaaa
    19381 cctgcatcgc gccttacccg tcatgaggct gcaatgattg ccactaccct acccgcacca
    19441 gctaagcgaa atccatcaaa accaagtgca tacatggtga gctaccaacg aaaaattctt
    19501 tggaatatgt ataatttagg tgaaattgac attaatgaaa aagaacagaa atagacacga
    19561 taattaatac tttaaaatgc aaagattatt attccccttt tggctaattg tgacttttct
    19621 tgtttgtttt accccttcaa aggcccaatt caacaaggcg tacttttttt acaaaggcga
    19681 ggagttaatt tcaagaggaa actatgctga tgccatacca gtactgaaca ccctgctaaa
    19741 tgttgactca accatacacg aggcatggtt tctgcggggc gtagcccgct actaccaaaa
    19801 cgatttggtt ggtgcaggaa acgattttag taagtgccta aatattaatc ctttattttc
    19861 ccaagcctac cagtataatg caatggttct gcagcaattg gggaaaagcg ttgaatccat
    19921 agagtgtatc caaaaagcca tcgagctgcg accagcatcg gcgcaatacc tttttaccta
    19981 tggtgttatt ctattccagc aggaacagta ccacgatgct ctttatgcct tcaaccaggt
    20041 gcttaaaatt gatgaccgta tcccggatgc gtgggttaat aggggaacca ccaggttaat
    20101 gctctcggat agttccggag cgttgacgga ttattctgcg gccataaacc ttaatccctt
    20161 taatgcaaac ccttacttac ggaggggtgc gctttatgcc cagcaatcaa actacccact
    20221 tgccttgcaa gatcttaatc aagccattgg gattgatagc acactcgcac aggcatattt
    20281 tacccgtgca ctcgttttct actaccaaaa acattatagc caggcattat cagacttaaa
    20341 taaggttttg cagcttgaac accgtaatac ccttgccctt tttaacagag cgattataag
    20401 ctatgaggtt ggcaaccccg aaagcgcaat tgatgaccta accagactat catcaattat
    20461 tcctaccaac gtgctggttc attactacct ggcctcaata cgttatgaac tcggatattt
    20521 gcctatggca ctggagaata ttaataaagc catagctatt tttccagagt ttgccaatgc
    20581 ttaccggctt agagccgaga ttaaaaccaa aaaaggcgat ttgaaaggtg ccgaagctga
    20641 cattgctcag ggcaatagat tagcgcaagc atacagtaat agggcaatca atgatatagc
    20701 ctcagtaatc gattccacag gaaaactaaa acggttacta tcacttgacg aggaactcga
    20761 catcacctca tcaattagta tggaacagct cagaacccga attctaacca ttgtttaccc
    20821 tataatcacc ctaaaagtag ataaaatcga aaatgtaaag cctaggaact ggcaaagttc
    20881 gaacctcagg cagattcttg ccacacagag ttgccgcggt tacagcctat tctttgaata
    20941 cgacaccatt aaccagcatt cacaattgga aaccatcgcc accgattcat tgtcggaaat
    21001 ttgtaaagcg atagcccatt tgcaaggcaa ctactatacg gccgcaatga aagaattttc
    21061 agaaacctta aaggctgata gttcaaacac tctttatagg cttgccgagg tgttggcaaa
    21121 aataaaaatg acccaatttg ttgaaaacgt taagaataaa agcttacaaa gcgggaaaag
    21181 taatatatcc caaaaaacaa ttgataacaa accctacatt actgctggta atgatctaaa
    21241 aactatagca ttggacaacg actttgaggg catagttcct tacaacatag gagtaatgtt
    21301 tatgcatact gaaaataatg accttgccat tgaatggttt tccaaagcta tcagcatcaa
    21361 cccccttata catggcgcgt ggtataaccg tggattactc taccttatca ataatgaaac
    21421 tgtaaaaggg tgtgccgatc tcagaaaggc agttaacata ggcaataatc aggcctctga
    21481 ggtggtagct agattctgta aaaagtaaaa gccctgctaa tttttatgca gggctttaat
    21541 aacagctaat actcaaatta aaagtataaa tcgcgctctc cagctttgat acgctcaatt
    21601 cttcgacgaa tctccagggt gttctgatgc ccgtcgagct ctccaagggt tttttcaata
    21661 agcttgtagc ctttctgctg aacctctggg ttggcatagt caaccaggta ctcagtaagg
    21721 gttaacaggg cgtttggggt gcagtaacgc tttataaagc caggcactga gaactccatg
    21781 aaatgttcac cggttcttcc caggcggtag caagccgtac agaacgaagg gatgtagtta
    21841 tggtcaataa gctcatcaat aatttcacca agcgagcggt tatcgttaat ctcaaattgc
    21901 tccttattaa ggtcctgatg ttcattctta gatgcatggt acgaacccaa ctcaagctta
    21961 gttcccccat caatttgcga tacaccaaac tgaataacct gcctacgaat atcggccggt
    22021 tcacgcgctg taagtatcat accggtgtat ggcacagcca agcggagtat tgcaactaac
    22081 tttacgaaat cgctatcgga aacaaaatac ttatcggtga tatttaaacc ggatgcagac
    22141 ttaatgcggg ggaacgagat ggtatgcggc cctacattat agcatgcctc aaggtgattg
    22201 gtatgcctaa ccaggccaag aacctcaaag cgccaatcgt agaggccaaa gagtgcccca
    22261 attccaacat cgtccagacc agcttcctgg gcgcggtcga gcgaggttaa tcgccactca
    22321 aaatctttct ttttgcccga ggggtggtac caggtatagg cctcagggtg ataggtttcc
    22381 tgaaagatct ggtaagtgcc tataccagcc tcctttacct tccgaaatcc ttcgatgtca
    22441 agcggagcgg cattgatatt aacccttcgg atctcaccgt tgttcttctt aattccataa
    22501 accaacctaa cggtgtgggc aatgtactca gccgagtact ctggatgttc accgtaaaca
    22561 agtatcaatc gcttttgccc gttatcctca agtgcttcaa cttcctgtac aatctcttca
    22621 tcggaaaggg tcatacgtat agcatccttg ttcgatgccc taaaaccaca gtactggcag
    22681 ttgtttgtac acttattccc tatgtaaagc ggagcaaaca gtactattct gttcccgtaa
    22741 atacgctcct tgagcgtacg agcgccctgt agtatctcct caaccaattc agggtcggtg
    22801 gtattaacca gagtagcaac ctcctcaagg ttaagcctat tcttatcgag cgactttgca
    22861 attatctcac gaaccctttc cttggtgggc tttgcgttgt ttatgtattc ccatatttca
    22921 tcggcatcga tgaaaggctt catgcgctca tcgggaattc ggtacttttc cggattaaat
    22981 ttcatggctc tatatcggtt taaaatagtt aacactatca aacttacctg agcctgatac
    23041 cagagggaaa agagatttta aaaaaaaact acaccccttt gagcattaag gttttaacct
    23101 gaacaccgct gagcttacca atgggcccgg taagggctcc aatttcatcg gttgttcctt
    23161 caagcacaag cgatattacg ctaatacccc tctgctggag cggtatgccc tgacgggcaa
    23221 taataatatc ggcatgggcc gaaaggatct ggttcagcct tgaggcgctc tcgcggttct
    23281 caacaagaat tagtgcagct ccgattctct tttccatcag actatctcct ttggatcaga
    23341 ctcattttct gaatccttgg tggcaaacat ctaccaacct cagatgcagg aattacattt
    23401 caaaaaacct aaatacttat acgattaact atgccccaaa gatacgttaa aatacaattg
    23461 gttaacaatg ataaatatca ttactgttaa taaattaact catcaacctg cttggctgcc
    23521 tgtgtaagct gctggaataa caacctgtta tcggagatat caattctatc agctatctgc
    23581 ttatgaaagt aaatataggc atcgctactg tgaagcatac catcaaaatt gaggatatga
    23641 agttcaacat tccttgtcaa aactgggttc aaatcggtat tatcgccatt tgaccaaatg
    23701 ataaccatgg cacgatacga tgatttattc cattccagcg aaacggcata aatgccctcc
    23761 ggtggaatga gcttgcctgc ctcctcaatt tgaaccgtta gggttgggaa acccacctcg
    23821 ccaaaaaagt gcgaaccctt acccaaagca ccaataatca tgtagtaatg gtcaaggtaa
    23881 gcatttgccc gttgtatttt tccctcaagt aaagccttac gaatagtggt ggaactaacc
    23941 gtttcctgct gtatatcctg ctcgggtatc tcctcaacca taaaattata ctccgaactc
    24001 aattttttga gctcctcata atcgccttcg cggttgtgtc caaaatgatg attgaaacca
    24061 actacgatgt atcgagcgtt cagcttacta atcaagtaat ccctgataaa ttgaaccgat
    24121 gaaatttttg aaaattcgag ggtaaatttt actatgatta gatgatcgag gccggttttg
    24181 ctaagcagct caattttttc gcgttgcgac aggataaaca tcaggttttt accagcagtt
    24241 tcagggtaaa gcactttgcg cggatgaggg taaaaggtaa taagaaccga ctcgccgcct
    24301 atgctttgag caatctcatt tatcctgtta ataattacct tatggcctaa gtgaacccca
    24361 tcaaacgacc cagtggtgac caccggatta gttatttccc ctataccatc aaaaccataa
    24421 tgtactttca ttactatcgt ttaccgggaa tagccctgtt taacaaagta agccactttc
    24481 tccatggagg taatcatatc gtactcctca aggtaaacat tctcaggtac aactaaagga
    24541 attgtggtga agttaaggat accctttata ccagctgcaa caagttgctc ggcaacttga
    24601 ttggcagtat cgggtggcga tgagataatg gcaatggaaa tatcgttacg ttttataaca
    24661 tcgggcaact ccttcatgtg gtagcaattt acaccagaaa ttaccctgtt aaccttgttg
    24721 ggatctacat caaaagcggc aactatatta agtttagggc gtttgccatt gaaataggta
    24781 gtgatagcgc gtcctaggtt acccatacca acaacagcta catttaatcc tactttgtgg
    24841 tcgatgatat cgccaataac cttgataagt tcctgtgcat cgtacccttt ctttttcatg
    24901 ctagagtagc caataagcat caggtcgcgc cttacctgaa ccgctgtgat attatgcaaa
    24961 ccggcgagct catgtgagta gatatgagtt ttccctcgtg ttaagcaatt cagaaaggtt
    25021 ctgcgatatt tgctcaatcg ctcaatggtt ttttcgggta ggtttgcctt atccatagct
    25081 caactattac atttttcaac acaaaactat ttaacttttt tcagttaaca aacggctgtt
    25141 taaaataaag tggatttaaa taccgtcgtt aagggtatcg gaaccgggta aagggaatgt
    25201 aatggtaaag gttgatcctg caccaggctc gctttgcaca tcaattctag cgccatacaa
    25261 atccactact cgcttaacaa ttgaaagtcc taagccgctt ccggttacat ttttgctctg
    25321 cggatttttg atgcgagtaa actcctggaa aagtttaggt atatcctcgg gttcgatgcc
    25381 aattcctgaa tcggatacaa ctagcgtaac ctctgatgct gttttgctga tactacactt
    25441 aacccaccca ccgggtttat tgtatttcac ggcattggaa ataaggttgt tgaacaaaat
    25501 ttccatgtcg tcggaatcgg caagcatgat aacgggttct gtggtttgca ggctaaggtt
    25561 gacgtcgcgc tgaatggcaa ggggagtcat ggtctcaatg gccgaacggg ccacgagggt
    25621 taaatcgatt tcacgaaggg ttcgctgccg tttgcctgac tcaatgtgcg tgaggtcgag
    25681 taggtccatt attagattac gcatggactg gattcgtgca agcgaacggt cgataaaatc
    25741 catgtaactg tcaatactct cgccagcctg cttctcctgc atcattttaa ggtagccctc
    25801 aacggcgtta agaggtgctt ttagctcgtg cgagagcacg gtaaggaact gaaaccgaac
    25861 ctgcttgccc tcacgatgga gcttgcgggt catacgccgc aggaaataat gcttggtgat
    25921 attctcaatg gacgacttaa gctcctgtgg agtaaagggc ttgggaacaa aatcgtaggt
    25981 accaatgttt gtagccctaa cagccaactc cagcgaagca tacgatgtaa tcatcattac
    26041 cagtatatcg gggtgattct ggttgatgaa ctgcaaaacc tcaaccccct gaatgcctgg
    26101 tagtttatta tcgagtaata caatggctgg actcgaacgc ttgataatat cgattgcctc
    26161 ctcccctgtg gccgcttcaa taacattaaa gcctatatca tcctcaagga atgggtagct
    26221 aaccgtgaag ttcctaagaa tacgtgttac accggaacga attcccggtt catcgtcaac
    26281 aacgagtata tcaacaactt ccatttttac accttaagca atttgagaat ttcacgatgc
    26341 agctggtcgg ggcgaattcc cttttcgagg tataaatcag ccttaatcca ctggcgttcc
    26401 tgttcgcttg aaatgccaaa cgacatgcct gtctcagccg atacagcagt ggctattatt
    26461 acgggaacat cgggatacat tcgttttagc ttgtagctaa gaataaaacc gctatcctcg
    26521 ttctccatca ttaggtcaag tatggctaaa tcgggtttga tattctttag gatattctcc
    26581 gcctcacgct gactttcagc ggtaaccaca tcgaagccca ttttttcaac atgaaactta
    26641 agctgaaaca ggtagtcggt atcatcgtca actattagaa tggtttttcg tgaattagcc
    26701 atagctattg cgtttagtta actattatcg tttcctaatt cgttttccgc attacggggc
    26761 agggttatcc taaaggttgt tccggtaggt cccttttggg ggtcggtgtt ggactcaaca
    26821 tctattttgc ccttgtgcat ctttacaatt ccgtaggtag tggcaagccc taatccggtt
    26881 cccttgccaa ttcccttagt ggtgaaaaat ggggtgaaga gcttgtccat attctcctcg
    26941 ggtatccctt cgccagtatc gattacgctg attgaaatct gatggtcatc gccgtcgata
    27001 acaattgtaa ggtcgccacc attaggcatg gcttcaaagg catttttgtt caggtttgta
    27061 agcacctgca tcatttgctc gtaatcgaga ctcgccatca ggtttgtggt tcggcaaacc
    27121 acattggtgg taacattctt tgggataaca acccctgcaa tgctatcctc agccaacttc
    27181 ttaacattaa ccttttccac cttaacctga ttcttgcggg caaagtttag caaaccgcta
    27241 acaattttct tgcaccgttc cgcctgcgac gatatcaact ctaagtcctt acgaagggga
    27301 tcgtccggtt tacactcatc gagcaggata ttgctgtaca tcaaaacaac acctaaagga
    27361 ttgttaagct cgtgagcaat accggccgaa agctgcccca tatgggcgag cttttcggaa
    27421 tgcttgagag cctgctgcat ttttgccagt ttctcattgg aaatggctag atcgttgatt
    27481 gactcatgca gttcctcaat agcgtaaggt aaacacattt caacctcggc aaggccttca
    27541 acaatagcta tagcgtgctc ggtgcaagta tcgtaaccac atgcgccgca gttcaggtga
    27601 tcgcgagggg taaacttacc catcttggcc aacactttgt ttatctcatc atcggtaggc
    27661 ttagctattc gtttatcatc gggattaaag ctacgcgtaa ggtcaagatc tttaaatttt
    27721 gatatggcta gctcccactc ttttagctgc tcaggggtta ccttattacg tgcataggaa
    27781 ctgatatagg ttttgcgctc aaaacgtttg cctccgggcg acatgccggg ccccattatg
    27841 caaccctcac agcaaagtag ttcaaggttt tgtcccttaa ggttacccga ttcccattcc
    27901 tttatggctt cctgaaagcc tattcgtcct tcagctactg tgatgttgcc ttcaattagg
    27961 tcatcaaata tgttaacggt ttgcattaac cccctactaa ccgggaatat cgccccacgg
    28021 ccagcatggg gtggatcaaa atccgatggt tcaacgcttt cgggcgttat actggcctgc
    28081 tcaaaaagaa cccgcaactc cctaaaggtt attgcctcgt caatctcaat ggattcatcc
    28141 tttttggcta tgcaaggacc aataaaaacc tgtttcaggt tactgtcaat ttgccttacc
    28201 accctgctta tggccaccat aggcgacacc acgggtgcta aattttctac taaatcggga
    28261 tggtagtaac ggatgtaggt aacaatagcc ggacaatcgc ttgagatata accctttcct
    28321 gacctatcct ccagcaacga tttgtaacgg ctagctacca aatcggcacc aaaagcaacc
    28381 tcaaaaacat gactaaaccc caaggccctg agcattccaa caaagtagcg ataatcatca
    28441 atctcggtaa attcagcggg aaaacttgga gctacaagag cagcaaccct ttcgccgctc
    28501 tgcagcatgc tttttacctc ggtgcgggtt agtaaaaaca cctttgcatc ctgactgcat
    28561 accttaacgc agttgccaca accaatgcac ctttccgata ttacatcggc ttggccattt
    28621 acaattttga ttgccttagc cggacactcc ctaacacagg tataacacac cctgcataac
    28681 tccttaatgg tgtataccaa tttcctcttt tcccgattca tctccatgct atagccgaat
    28741 tgttccattt aaagtaaaac ttcccgcttt gaatatcggg tgtatagaaa ttttttggct
    28801 ttttcactac cggtttcccc taaaaactca tcgtatatgg attgtacggc agggttacgg
    28861 gttgggcact taattccttc cgcctcatca atttcaataa ttgctttggc cctcaactta
    28921 acatactttt cctcgtcaac atagggttgg cctcctccat ttacacaacc accgtggcaa
    28981 gccattacct cgatgtagtc cagtttaaca tcgcttccca tcaaaaccgg gagaatagaa
    29041 tgtatgtttt ttaacccatt aacaactgca aaggtgaatg tttgcccagc ggcattgaaa
    29101 gagaatgcct ttaccccaga tagtatttta acctcgttga ccttactatc ggtaagctct
    29161 tttgaggtta gtttatggta aaccacccgg gcaagcgcct cggctgaacc tcctgatatt
    29221 ccaaatagct ttccggacga gctacgaata ttaaaaggtt tatcgggatg ttgcgattca
    29281 atcccctgaa tatcgatacc gtaaagttta atcagcctaa ccaattcacg tgttgataat
    29341 accgtatcca catcagatag gccacgggta gtcatctcct ccctttgagc ttcaaacttt
    29401 ttggccaaac aaggcattac ggaaacggag aaaatttttg atggatttaa ttcattttcg
    29461 gcagcaaagt agttccttat taacgatccc attacctgct gaggcgattt aacggcactc
    29521 agtgccggaa tataatccgg ataccactgt tcaacaaact taacccatgc cgggcagcat
    29581 gatgagaaca tggggagatt actgccattt ccctttcgtt caatgagctc agctgccatt
    29641 tcaattgcag cgacatcggc agcaaaagca gtatcgaaaa catacttgaa cccaattttt
    29701 cgcaatgcat tgtacagcaa accattaaca tccttgctgg gtttaagccc gaattcagta
    29761 gctaccgaaa ccgaaattga aggggcaatc tgcacaacag ggattaaatc aggattgctt
    29821 agggcatcca ttacttcggc aacattttga cgctcatgta aagcgccagt agggcaaacc
    29881 aatacacact gtccgcaacc aatacagttt gagaagttta aatcgcgcga caaagtggta
    29941 cccacataca tgttgcatcc cctgttgaca aactcaaagg ttgataccga ttgaacctca
    30001 tcgcaaaccc ttatacatct tccacaaaga atacacttag ccggatcgcg gacaatactt
    30061 gggctggaaa gatcaagctt ttgagtaaac gactttcgta aaaccttacg ctcacgaatg
    30121 tgtagctctg ctgctaaatc ctgtagctcg caaaagccgt tccgttcaca gtataagcag
    30181 tcgtcgggat ggttggcaag gagcaactca accagggttt tacgggcgcg caccactcgg
    30241 ggtgaatggg tcataattcg cataccattc tccaccacat gcgagcatga ggtaacaagg
    30301 ttatctcgac cctcaacctc aacaacgcat agcctacatg cccctgatgg ggttaaatcg
    30361 gtcatgttgc aaagagtggg caccctgata ccgttacggt ttaaggcctc aagaatggta
    30421 tcgcctttat gggcttgtat tgttctatta tttacttcga tggtaatgtt catagcagca
    30481 aaaactttac ttgaagaaaa cagctccaaa cttgcatgta tcctggcata taccgcatcc
    30541 tatacaacgc tcctccacaa taaagtaggg agaacgtggt gtcccatata tggcctgtgt
    30601 tggacacttc ttggcacagg cagtgcaacc cgtacaacgc tcaatatcga tgtaaaatgt
    30661 ccttaaatcg cgacaaacgc ttgccctgca cttccgatca aagatatgct cctcaaactc
    30721 atcgcggaac gattttagag ccttaaggat tggtttgggt gccgtttgcc ccaatccgca
    30781 tagcgatgtg tcgcgcataa cctcagccag ggtttcgagc tgaataacac ccttaaaccg
    30841 ttcaagggta ttatgccctt cgttactaac tggtcgtttc gatatgttat cgagtatttg
    30901 gtacatccgg tttgatcctt cacggcaagg aatacattta ccgcagctct cgttacgaat
    30961 aaagtgcatg aagtatttca ccaggtcaac catgcacacg gtattgtcaa aaacctgaac
    31021 accacccgat cctaaactta tccctcgttt cggaagttca tcaaagtcaa taggggcatc
    31081 aagttcatcg gctgtaacac tcaccccaga agggccgccc aggtgcaatg ccttgaaagt
    31141 ccttccgtcc tttacccctc ctgcaaggtc aagcaaagcc ccaaggggtt caccaaaggt
    31201 taactcaacc atacaggttt gagacgcctg accattaatg gagaaaattt tcgttccctt
    31261 tgacccctca gttcctaaag atgcaaacca ctgaggccca ttactgataa tttcggggat
    31321 attggctaaa gtctcaacat tattgataac agtgggcttt ccatttaatc caaattcagc
    31381 agggtaaggc ggttttatgc tgggcattcc acgtttaccc tcaaggcttt taatcaaggc
    31441 agtttcctca ccgcatacgt aagccccggg gcctttacgc acctctatat cgagcgaata
    31501 gccactccct aaaatatcat atccaagtag tcccacctca tatgcctggt caatagcatt
    31561 ctgaattctt tgaatggtaa tcttatacct atcgcgtatg taaataattg ctttacttgc
    31621 cgacacggca tacgagctca aaataattcc ttcaacaacc ctatgcggat cgctttcaat
    31681 cagcagtcgg tccatgaatc cccccgggtc actctcgtcg gcattgcaaa tcaggtatcg
    31741 atcgctggtt gcaacctgta acgctttgcg ccacttggtg cctgtgggga aggctccacc
    31801 gccccttccg cgtaatccgc tctcctcaac tatgcggcaa atatcgtcgt aggtgtactt
    31861 cctgatagac ttagccaaag cgctatatcc gccatgggca atatactcgg caatgctatc
    31921 ggggcaaatg atacctgcat ttttaagcaa cacacggttt tgccttttaa aaaagggaag
    31981 ttcgtttcta aaaggaattc catgccaagg ctcatgaatc tcggaatagt actggaaaat
    32041 ggcattctca ggctgaataa aaccattaag agctccatcg agtatggagt taaccctttc
    32101 aggagttaca ttggcaaaag ccactctagt cctaccgggc atctgtactt caacaatggg
    32161 ctcaagagag cataaaccat ggctaccaac acatattaca tcggcatcaa cagaataatc
    32221 gtctaagtac tgctcaatag ctgacctagt tttaagcgct cctgcaacaa tggacgatgt
    32281 agttacgcca acatatatta ttggctgcct aacccgttca tgccttaatg cgctaagctt
    32341 agcgtttacc tcggtaagcc ataccccatc gtgaaggttt ggaataacag catcttttat
    32401 aaaagatatt tcagcttttg aatcgcaccg agccatgttt actcttgggt taaatcgttc
    32461 aaaatatttt taagcttttc ggggtcaacc tcacggtaat actcatcgtt gacggaaatc
    32521 actgggccat gttcacatgc acccatgcag gttaccacct caaggctaaa ctgcccattc
    32581 cgggttgttt gaccattttt cagcttcaaa ttatcctcaa ggtaaaccat attccacttg
    32641 gcacccttta gcctacacga ggtgccatta caaaggcaga tatgattttt ccccttagct
    32701 tcaaacttaa actggttata gaatgtggct aagccataaa ttttggaagt tggcatgttt
    32761 aactttcgcc ctaccagaac aattgcttcc tcgttcagac ttcccaactc gttttgaata
    32821 tcctgtagaa ttgggataag gctatcggaa cctaagtagc tgtagcgttc tagaatctcc
    32881 tcaattttct cggcgtttga catagcttta aagtaaggta gtaattagaa ccatagttaa
    32941 cccaccattc taattcatct gttaaataac taacagagac atatattttt agtaatcaat
    33001 tagttacaac aaataaaatc ttgttaatca cggaacaatt taataaaaca ttacaaaaat
    33061 tgttcgccaa acaccaatat tttatctatt taaaacgatt acagataagt gatttgattt
    33121 aaaaaatgat taaatttcag gcataatttg taaaccaaaa cctatcttag cattttgtaa
    33181 tagtgttata ccgataacat taagatgagt aaacgagccg aattaattat atgtttgggt
    33241 agttcctgct ttgcaagggg taataaggcc acactcaagg caataatgaa atttttggac
    33301 gatcacgatt tgaaagacaa ggtaaacttc cacgggggac attgttttgg taattgcgcc
    33361 gaaggtccta acatctctat taacggtaaa atttataccg gggttgatga aaatggaatt
    33421 attgacattc ttacaaatga gctactttaa ggagacatat gtcacaactg tttagaatag
    33481 acgagcaaac ctgcattaac tgctacgcat gcgttagggt ttgtcctgta aaagccattg
    33541 aggtgaaggc aggccaaaac tacgctcgaa taattgatag ccgatgtgta ggatgtggga
    33601 gctgtttaaa tatttgtccg gttaatgccg taagctatag atcctcaaaa aaggaggttt
    33661 tggagctgct aaaaggcgac gaacccgttg cggctatttg ttccccaagt atttcgggtg
    33721 aatttgtgga tattaccgac tatcgtaagt ttgtacagat gattaagcag ctaggtttta
    33781 cctacgtttg cgaggtaacc tttggggttg acctggttgc gcgggaatac aaaaagcttt
    33841 tcgaagattt taaaggcaag tactttatta ccagcatgtg tccggtggtg gtgtcgatgg
    33901 ttgagaaata ccaccccgag attgctcaaa accttgcccc cataatctcc ccagttgtgg
    33961 ctacagccat ggcggtcagg aaagagtatg gctctaacgt tagagtagtt cttattggcc
    34021 catgcatcca aagcaaagaa gaaattagac tctacgacga cgaacgaaga attgatgaaa
    34081 acctcacatt tgttgagctc agggaacttt ttgatgagtt taacatcaag gagagcaatt
    34141 tggaatactc tgaatttgac gagccaattg gatataaggg ttcattgttc ccaattgggc
    34201 gaggtatact gcaagctgta gatattagcg aggatctact caccggtaca gtaatcagca
    34261 ccaatggtcc aaacaacacc ttacaagccc ttgagcaatt tgaaaccagc atcgatctga
    34321 taaacaggca tttcaatctt ttctactgtg aaggttgctt gatggggcct ggaacctcac
    34381 gaggtggaaa aaagttcatc aggcgaacaa tggtggttaa ctacgctaac aagcgattaa
    34441 aaacctttga tcgcgaaaaa tgggaaaaaa atatcgcaaa aaatagtctt gggaatttcg
    34501 caaggggttt cacaattgat gaccaacgcc ttcctacacc caacgagcat aaacttgaag
    34561 aggttttaaa ggtgattgat aaagagtttg ttgggcgcga ttcggggtgc gaggcatgcg
    34621 gatactcctc gtgctacgat tttgcctcgg ctgtagcatc ggggcttgcc catactgata
    34681 tgtgcttgaa ctttaccctt aaaaagcgcc aggaatacat aaaaaccctt caggccacta
    34741 atgaaaaact cgctaaaact caggaagccc ttaaggagtc agaaaagaaa gctagaaccg
    34801 agcaagaggc agcccgggaa gctactgaaa ccattaccgc catgcttaaa aaactgcctt
    34861 cggcagtagt gcttatcgac aaaaaaatga aaatcctcca gagtaaccaa agttttattg
    34921 atctgttggg cgacgatgct ttggagatta atgagataat ccctggactt gttggtgccg
    34981 accttaaaac cctgctccca tacaacatcc acaacctttt taccagcgtg ctccaaaata
    35041 acactgatat cctgaaccgc gatgtgatgt acaaggataa gctgcttaac ctttcagtat
    35101 tcaccattaa acccaatagc attgttgggg ctgttatccg cgatatgtat gcccccgagg
    35161 ttcagaagga ggaggttatt aaacggatta ccgaagttat tgataaaaac ctagccatgg
    35221 tacaaaacat cggattcctg ctgggcgaag gtgcatcgga aaccgaaaag atgctcaact
    35281 caatcattaa aacataccgc gagggaaaaa ataacgacga taaagcatag tagcctaaaa
    35341 ccatgtcgag taagtttttc atagaggtaa actgccaaca aaagaatcac gacgaggagc
    35401 gtatttgtgg cgacgttttt tactcgcgca ggttacagga cgaagagcgc accataattg
    35461 tgctatccga tggcatgggg catggtgtta aggcaaatgt actggccacc ctcacatcca
    35521 ctatggcact taacttcacc atagagcata aggaggttaa cactattgct gaaattatta
    35581 tgaataccct tccggtttgc tcagtccgca aaatgagtta ctctacattt tccattgttg
    35641 atattgacca caacggcgaa actcgcatac tggagtacga caaccctcaa tgcctgatca
    35701 tgcggggaac cgaggaactt gaaccagagt ggcaatgcat aatccttaac agcgaaaaaa
    35761 atgcgggtaa agagttaaag ttctgctcgt ttgttccgca aaaggaagac cgaataatcc
    35821 tttggaccga tggagttgct caatccggac tgggctcagc cgattaccct ttcggctggg
    35881 gcgacgaggc acgcgaattt gtgaaacgaa tggttaaaaa tgaacccgat atttcggccc
    35941 ggaagctaag caccaaggtg gtaaatatgg ccagcctcaa cgatggatat catcccaagg
    36001 atgatacctc gtgtgtaaca atatacttcc gtaaccccag aaaactcctt atttgcactg
    36061 gccctcccta cgaaaaggag aacgacacga agcttgccaa gatatttgac acctttgagg
    36121 ggaaaaaggc aatttgcgga gctactacag ttgatatcat tgcccgggag ctaaaacgaa
    36181 gtgttgagga tagcttagaa tttaccgatc ctgatttgcc tccaattagc caaatggagg
    36241 gagccgatct tgttaccgaa ggtattttaa cccttagcaa ggttaacacc attcttgaat
    36301 cgtataacga gaacacaaac cttggtagag gccctgccga tgaacttgtt aaacttattt
    36361 tggaaagcga tagcattgaa tttgtgattg gaacctgtat aaacgttgca caccaggacc
    36421 caaacttacc tgttgaactg gaaatacgaa ggactgtggt aaagcgaatt gccaatatac
    36481 ttcaggataa gttcttaaag gaggttaaac tccagttcct atagaaggct taggctgctt
    36541 ttaccatttt tggaatatca atattttaac ctaacaagta aataattagt tcaactgttg
    36601 acttttacca acatttttac aatatttacg ttaaaatcat tgttaatcct taaaactatg
    36661 cgcagcttta ggtttttcct tttgaacatg cttattgcta cttacttttc agctgtaagt
    36721 tacggccagg attggaattc aattttcact ctggaaaccg acgctgaata ctacatggct
    36781 gagggaaact tttctaaggc agccgacacc tatgtaaaag ccctaaaaaa atttcctgaa
    36841 agtgccaacc ttaaatttaa aattggatac tgcttgctca aaacccccga cagaaaatcg
    36901 gaagccatta gctaccttga agaggctgta atgaaaattt cggataaata tgatgcaaaa
    36961 tcattaaagg agcccaacgc cccccccgaa gccctttatc agcttggtat gtcatacatg
    37021 tatcaaaatg attttgacaa tgcgctaaaa tcgttcagag agtatcaggg ttacatcaaa
    37081 cctaaagatg aacatgctaa actcatggcc gagcatgcaa tagcgagttg tgaaaatgca
    37141 aagaaatttc aaaaaagccc aataggcatt aaatacaacc ggttaggtcc tgattttaat
    37201 aatgaaaacg acaactttaa tgctgtggta tcgggcgatg gaagtacgct tgcttacact
    37261 acccgcacct cctcaggcaa taaaatattt gttgccaaaa aaggggagga tggcttgtgg
    37321 gctaaaccag ttgatataac ccgcaacctg ggtagtaaaa acctgaccac atcataccta
    37381 tcgtatgagg gaactgaact ttacctcata gagaatgacc ctagaaattc ggaaattgta
    37441 gtaagttttt tacagaaacg aaaatggtca tcgcccgtaa aggcagccaa acccattaac
    37501 tcaaaattca acgaaaccca tgtatgcgtt acccgcgatg ggaataccgt ttacttcact
    37561 agcgatcgca agggcggtca gggcggatac gacatatggg cttctacaat caataacggt
    37621 agatggagcg atcccataaa ccttggcccc aacgttaaca cccctttcga tgaggcaacc
    37681 ccattccttt cacccgatga aaagtatctt tttttcagct cacaggggca caataccatg
    37741 ggcggattcg atgtgttttt taccaaccta caaggaagtc ctaatgttaa gaatgcaggg
    37801 tatcctttaa atactaccga cgacgaaatc ttttactttc caaccggctt atcatcgggt
    37861 tatattgcaa aattcagtcc cgatggtttt gggaaacttg acattgccga ggttgaaata
    37921 atccccatgg ttgatgttaa gcttaacctc atgcttgccg acaatgctcc tctggataaa
    37981 tactacaacg ttaacgttac aaataattct acaggtgaga tagtagaaaa acagtcagta
    38041 aaaggtaaaa cacagcttag cctaaaggta aaacccggcg attatactgt aactgctcag
    38101 ggcgaaggct ttgaggaaac taaaacccca cttagcattc cagccaagcc cgataaatcg
    38161 gaataccagg caagcctaat gttaaacccg agagtagttc agcagcccgt tgcccagctt
    38221 gaacccccca aggaagaacc taagcccgaa gttaaacctg aggtaaaacc tgaacccaag
    38281 cctgaagtaa aacctgaggc tagagctacc gataaaccta aggctgttga acccgaaccc
    38341 aaaaaagaac cggttgtaaa acaagcccca aaaaaggaac caaaacctga agttgaaaag
    38401 ccaaagcctg aaccaaagcc tgctaaggca ttgcccccaa cccctccccc aacagcagca
    38461 ccaacaccca gaatggactt caataaaacc tcatcaacaa gaactcttgc taaaccaaca
    38521 tactctgtgc agctaatggc ctcactatat cctgttgact ataattactt taagctcgac
    38581 agcattagcg taaccatttc gcccgatggt tactataggt atagtgtagg aaatactgat
    38641 aatatcaagg agattgaaga gctacagcag aaggttcgtg ctcatgggta tagcaacgta
    38701 tgggtaagaa taaatcggga acacccggga tataccattc aattcctagc attacagaag
    38761 cctaaaagca taaagcagtt tactgaactt tccgatgtaa tggtttaccg tggaaacgat
    38821 ggagtgtatc gttactgtgt tggaaagtat tccacacccg aagaagcctc ttcggaccta
    38881 gccaagatta ctggattagg ctattcgggg gcatttgtcc gtgaactagg gaaataaagt
    38941 ttagaaagaa agtataaagg ctgcctaaat attaggcagc cttaaatatt taacgaattg
    39001 aggcccgaaa tggaattctc aatgataatg aaatatttcg accaggatta taaagatcaa
    39061 cctcctttaa tgttgacaag tgatcaacat actttttatc gagtaaattt tgaactgtta
    39121 aatttacttc aagaggttga ttgcccaacc taaaactact ccccattgat aagttaacta
    39181 atatataatc tggcgttact gtttcacctg gatctatatt attttgggca aacgcatagt
    39241 taatcgctat cagcaagtat ggtttacttc ctaaagcaaa cttctttccc ataaaaccgg
    39301 tctcaaaagt tagcttatta gctggaataa aagggaggta ttctccatta cgtttcttgc
    39361 caatcaccaa agaataatta gttttaaaat ggagccactg aaccgatttg gggtgaaaat
    39421 gaagacctaa ttcacctcca tacaagtaag cgtcatcctg cgaataccga tactctggta
    39481 agcctccgtt caatgcacca gtgggagcaa tgtaaatgta atttcctatt gaattaacaa
    39541 aaccggcaac atcggctaaa aagttatcat tgtgataatg agcactgaca tcgtactcaa
    39601 cagatttctc aggtttaagg gaaatgttgc caacttcgta gcgaccctca tgggggccat
    39661 ttgatgtaag ttctgctagg tttggatttc ggtaagcagc agcgccattt gctcgtatta
    39721 aaaagtattc atttaatcta taggttgcgc caacagaacc agtaatatta ttatacttcc
    39781 tgtctacact tgctctatac tcaggttcac ttggaatacc aacctcattg gatttaattt
    39841 gctttgcatc aaacctcaat ccagcttgaa atcgaaacct attaaaggaa cgttgtgcta
    39901 atccaaagat tgagtatcct gaactaaatg cattgggaag aagtatggtt tcgcggttat
    39961 tgatgtttat attccattgg ttcacaccct gtgcacctaa aatgtaatcg ttgcctggat
    40021 ttgaagggaa caatacctta gcctcgtaaa ttaaagttgt tagacgcatt tgtaattcgt
    40081 actcatttgg gttacctaaa tgggcaagtt cagttgattg caacgaagta ttaaaatcga
    40141 cctttgtttt acccaaaaag attttggatt gctgggaaac cagatgggtg ccaaactgct
    40201 ggtaataaag tgagggtttt cgtccccgac tagcaattaa atcaatggct tcctcttcca
    40261 ccagtcctaa cttttgcatg ttgtagtcat aataaatgtt tgttgacaac atttttccat
    40321 taaaaccccc attgagttta accgaatgct cataaaatct ggagtttgga acaaactctc
    40381 ccccaccctg caggtaatcg gaatgagttt tttgacctac ccgtattcct ccatgaaatt
    40441 gattatctga aactttgaat cctaaattat tgaccatacc caaagaattt gagaatagtt
    40501 gcaagttgta gtccccctcc agcttaccaa cagtagccgg tctctcttta ataaagttaa
    40561 gtaccccacc tatggcatcg gaaccaaaaa gtagcgaggc aggtcctttt atgacctcaa
    40621 ccgtctcaat accaaactca tcgattccta atgggtgatg gtttgaatac tgataatttt
    40681 cgaatcgaac gccattgttg agcaccaaca catcgttcat ggatagaccc ctgataacgg
    40741 gtttagcaac accattccct ttacttatca tgtcgattcc aggcaccgta gtaagcagct
    40801 ccataaaatt cggcgatttt atcaagtgat gctcatttaa cctcagaact tcaatcttta
    40861 ctgcattctg gtgctgtgtt gtactatatc cagtggatac aaccaccctt tcggcctcaa
    40921 cacttgcagg aataagaaca acatctaatt cgaattgcgt cttccctaaa gtaaccggta
    40981 caatttgact agcgtatcca atatgcgaaa tctgaatatc aaacttaccg tttggtaaac
    41041 ccgtaagttc aaattccccg cttgaatttg ttgaggtacc ttttaccaac attggaataa
    41101 aaacattagc tccctcaagg ggaatcttgt tctcattgta tactattcct cttataatat
    41161 tttgcgccaa aacattattt ccaaggacaa atacagttat tatgaataac tttattaatc
    41221 tcatgtcaaa atatttttac tagtttattt actttttaac taaacaactt ttaaactggc
    41281 atgataccag catatcaatt agattaaaaa aaaacttcat atgcgattac aacataacaa
    41341 tcaatgttat atcataatac atccaataca gttaatcatt gattacttga gtaaattata
    41401 accccaatat gttaatatga aagattacta gaatatatta aatggtggcg cacgtatttt
    41461 tattaattca aaactatcaa taccaacatt gagagttatt aaatccgata tttctgaaat
    41521 aatatttaaa taaatctcaa ccttaggtaa aacaacttta gggtcaaaaa ctattaactc
    41581 atactgaaaa actaaacatt tatcgtggta atgagtaata tttattgatg aattatggtg
    41641 ctcattttta tggttatgat gatgaaatag tgaatcgcct atctttgaaa caataggtag
    41701 cagaaaaagt gtcagaagaa taagggaatg ttttgttttg ccctttttca ttaaatagat
    41761 atatttatct taaaacctac caaaaatcga tttgttcaat tgctattctt caacagtaag
    41821 ctcataaccc ccttttacaa gcacatttga aagggttgaa tcaaatactt gaacaatggt
    41881 gtcctgaatt aacccaacct gtataggaac ttttttaaaa aagtagctca gtgaatcgct
    41941 tccattcagg gtgtaaacca tatgcccagt ctcaatttca tgcaatgctg ttcttggcag
    42001 tgcccaacct tcaacaaaat tggtgaaaac cttagcagaa acccttgtgc cacttatcaa
    42061 aactaaattt gcgggtaaaa ttatagatgc aaaacattca acagccttat tttcagtgtt
    42121 ggcatcgagc ccaacagaaa taacgcttcc ataaacttta ccacctccag gcaaactaat
    42181 ttcaactttt tgtccgggtt ttattgagtt cacttggttc agaaaaataa aaaactttaa
    42241 tagtaggctt tgggtattaa ttacttcggc caagggctca taggttgtta cgggcttacc
    42301 aattgatgtt tctaccctag caactgtgcc ctcaataggg gaaaccaggt atgcctcgtt
    42361 tgaaaattct cctttaatta tttcatcggg atttagccct aacttactaa gcaaaattcg
    42421 gtgggagtgt agcttggcat caagaattcg gtattcgctt tcggcttgta aaagttcttt
    42481 ttgcgaggct attccctcct ttgatagttg tgatatcctt tcaaagttag cctttgctat
    42541 tctatgctga ttgtaggttt caacaaacaa ggtttgagta tttaaaatct cgggtccatc
    42601 tattgcaata agtgcttgcc ccttctttac tactgacgat gggactactt tcacagactt
    42661 aataactcca ccaatgggtg aggtaatgta agctttggca ttggggcttg gttcaataac
    42721 gccttgtacg gcaatttcgc tggcaaattt taccattgta ggattgccaa ttgccatatt
    42781 cattgcttta aactgctcaa cagaaattac aaccaagcta tcggttggct gattttcatc
    42841 gcttttatta ttttttccag agcaagacat tagcaatact ccaaaagtta taattaataa
    42901 aatattaatc ttcatcatac ctcaacttaa aaatatttaa cttttaaacc ttacatagtt
    42961 acttgcgaaa aagattccaa aactttaacc cattaaaacg ttcaaatact gcataaagca
    43021 acggaagcgc tatcatggta agtaaagtag aagtaataag cccacctatt acaaccgatg
    43081 caagcgggcg ttgcacctcg gcgccagccg atgtagatat agccatgggt aaaaagccca
    43141 tggcagcagc cgaagcggta agtaaaacgg gtcttaacct atcgcgggtt gctctcatta
    43201 tgcgttccct caaattttct actccctcat cctttagctc cttaagatgt tcaataagca
    43261 ctattccgtt aagaactgca accccaaaga gagcaataaa accaaccgcc gccgaaatgc
    43321 taaagggcat tcccctaaac catagcatca atattccgcc tattacagaa aggggcaccg
    43381 cggtatatac caggatagca tccttaagcg aatgaaaagc aaagtgaagg aaaataaaaa
    43441 ttaaagccaa ggctaccgga acagccacta acaatcgctt gcgagcattt cgaagattct
    43501 caaactgacc gccatactca acataataac cagcaggcaa cctaagctta ctgtcaagaa
    43561 ttttctgtat gtccttaaca accgattcca ggtcgcgatt gcgaacatta acacttacta
    43621 caaccctgcg atgagtatta tcgcgggaaa tttttgcggg gcctttggaa tattcaactt
    43681 gagccagttc acccaaaggt actaatcccc caccaggaac cgatactaaa agatttttta
    43741 cattgtcaat atcatgtcta tagctatcgg ataatcggac taccaaatca aaacgctttt
    43801 cgccctcaaa aatattacca gctgttgccc ctgcaaatgc tgccgagacc actctgttaa
    43861 gctctgtaac cgatatacca taaaccgaaa gtttatcgcg gttatacctg atgctaattt
    43921 ggggtaatcc tgccgtttta tccattatga tatcagcagc cccgggtaca ccatcaataa
    43981 gtttagtaac ctcaattgct ttggcattga gcacacccaa atcctcgcca aaaattttta
    44041 tagccaagtc agctctaacc cctgttatta actcattaaa gcgcatctca atgggttggg
    44101 taaactcata ctctacattt gggattaccg agagtgcctc cttaaaccgg ttagcaagtt
    44161 cctccttgct tttagcgcta acccaatact ttttgggttt aagtttgatt atcatatcaa
    44221 tttcttccat cgacatgggg tcggtgggca cctcggctgc accaattcta ctcacaatct
    44281 tatcgacctc aggaaatttc tcaatcagaa tcttttccat catggtggtt agctctgtgg
    44341 ttcgggttaa tgaggttccc gtttttagca caggctgaat aacaaaatcg ccctcatcaa
    44401 gtgttggaac aaactctgca cctaggcggg tgaaaaccca accggaaaaa agcaacatgg
    44461 caaaggctaa acccaacacc catcgtttat tattatatgc ccattcaatt acaggtttat
    44521 aggaggaatg cagcaaccac ataacccatc gggtaacagt ccaattactt ttctgcggac
    44581 gcagaaaaag cgatgctgcc acgggtaacc aagtaaaccc aaaaaacata gctcctagca
    44641 acgcaaagct aaacgacaag gccattggcc tgaacatctt tccttcaact cctgcaagcg
    44701 aaaggattgg gataaatact ataagtatga tgatttgacc aaatattgcc gagttcatca
    44761 tttttgatgc gccctggaac gagatggtat caaaaatttt cttacgctct gtgttttcaa
    44821 taccagtaat ttcattggac ctcagcgtga gtttcattac aatgaattca acaataatta
    44881 cagccccatc tataataatt ccaaagtcaa gcgcgccaag gctcatcagg ttggcatcga
    44941 taccaaagat atacatagct gaaatggtga atagcaatgc cagtggaata agcgatgaaa
    45001 tcaccagtgc ggcccttaaa ttcccgagta tcagtaaaac caccaggaaa actatcaagc
    45061 agccaaatat caggttctca agtatagtgt tagtagtttt tgagattaac tcgctgcgtt
    45121 ccacaatggg gttaatataa accccctcgg gtaaatgctt ttgtacctcg gcaacacgct
    45181 gcttaaccgc ttcaatcact ttattagagt tagcgttttt aagcatcatt atttgcccca
    45241 taaccttttc gccctgacca tttgcagtaa tggcaccaaa cctgttggca tgcccctcct
    45301 gcaccttagc cacatcggca atacgaactg aaataccatt aattgtggaa ataggtattt
    45361 gctcaatatc agcaagtgac ttcaacaacc cctcgccgcg aacaaagaaa ctttgattat
    45421 cgcgctcaat gtaagcacca ccggctacgc cgttgttttg ttccaaagcg ttaagaacat
    45481 caatgataga cactccatac tgggctagtt tttgcggatc gacagccacc tcatactgtt
    45541 tgaggtatcc accccaggta ttcacctcaa caacaccagg aatgccggat agctgacgtt
    45601 ttacaaacca atcctgaatg gttcttaggt cggtggtggt gtacctggtc tcatatcctg
    45661 gctttacgtc gagcacatac tggtatatct cccccaaacc ggttgagata ggccccatgt
    45721 caggttcacc aaaaccttcc ggaatattgg ctgctgccgc tttaagcttt tcagctatta
    45781 gctgccgggg caggtaagtt cccagattct cctcgaacac aatggttaca acagataagc
    45841 caaattttga aatcgatcgt atctctttca ctcccggaag atttgacatc tcgagctcaa
    45901 ccgggtaggt aatgaactgc tcaacgtcct gggtcgatag gttgcggctg gtggttataa
    45961 cctgaacctg gttattggta atatctggaa ccgcaccaac agggatttgg gttaaagcat
    46021 atacaccaaa tagcgctatg gttagtgtta gtaagattat aattagcttg ctgttaatac
    46081 taaatcttat aatcctttca atcatcttaa tttatatatt aacacatcta cattaaacaa
    46141 tatttctttt taaatggttc aaaaaggcat tattaaacct tgattcccat aactaaaaca
    46201 tcgtcagttt gctcagtaaa acctcgccac tgatcaaagg taatggatag caactgctca
    46261 atgtatgtat gcggttgctg gttgttatca accagtaaac tcttaagacg tgccgacgag
    46321 aatttttgtc ccgtttcagc cccaaactga tcgcaaaagc catcggtgaa tgcaaaaatc
    46381 agatcgttct ttgagagttt tacccgttga ttattgtaaa gtaccttttc cttatcccac
    46441 ccaatgcggt agatatcagg ttttaatttt accacctccc caccgctgat gtagtaaagg
    46501 ggtagcttag ctccgctaaa atctaactcc aatgtatgat ggttgaaaat agccaaggct
    46561 atctccatgc catcgtgaat gcttttttgg gattgggtaa agaactcaac aacgtgagcg
    46621 ttggtaatgg taagaatttg tgcaggattt tgataatcac actcctttac tgcattccta
    46681 agagcattct cgcaaataac gctgacaatg gcaccgggaa caccatgccc agtacaatcg
    46741 gccacagcaa aaaccgatac gtttttatgg tgacaaaacc agtaaaaatc gccactaaca
    46801 aaatccttgg gacggtaaaa gataaaggca tcgttaaaat atttcttaaa atcatctaca
    46861 ctaggcaaca ttgatgtttg caaaaacttt gcatacataa tactatccat tatctgcctg
    46921 ttcttatcat caacaatatc gagttgttcc ctgagctcca cggtgcgctg ttcaaccata
    46981 gcttcaagtt cctcttgcct ttcctttaaa tattgtatcc tacgattcac ataccaataa
    47041 attaacaacc ctatggcaat gatggataaa agcctaaacc accatgttgc ccagaacggg
    47101 ggagcaatat atattggcat cgatataacc tcgcccgacc aaatgccatc gctattggct
    47161 gctttcaggt gtaaacgata agttttcccg ggctgtaggt tactataaac cgcctgccgg
    47221 ttcttgctct caacccaact gttatcaagt ccttccaaaa accaggcata ctttacaccc
    47281 tgtgggtttt taaaactaac acccgcaaat tcaaaggtta aatggttttg ccagtaacta
    47341 agttttagct gggaagggag tccggtaagc tcatcaatgt cagaggcata atcggcccaa
    47401 ggggtgggtt gcgagcgtag gagtagattg gtaatgctca cctttggggg aatgttatta
    47461 attttatcga ggctcttctg gtaatgggca agtccgccgt ttgtggctat ccaaaagcca
    47521 tcatatccat cggctaacac gccatttagg ttgatttcta aatcgggtaa accctcgctg
    47581 gagccataat gcctcaaatc tattttgccc ttgctatact cagcgaggct aagcctatcc
    47641 aatcccaggt tggttccaat ccaaaggtaa tcgctatcgg catagagtac gtaaatcaga
    47701 tttgatgaaa caccatcggt ctccttaatc tgctttatgt ttgaaccatc atagtgaaaa
    47761 acccccgcta ttccgtgttc caaaccatat gtcaccttta aaatcttgta ctattgttga
    47821 tatttcagcg tttttatcga taccagtaaa cttaaagggt ttgtcgtttt caataaagaa
    47881 aataccgttg aaccgggttc ctacccacat tcgctttctt aaagtatcta tctgtaactt
    47941 ttggacaaaa acgcctcctg tatcgctatc aaaaaggggg aaatggttga aatgaattcc
    48001 atcagtggca taaaacaagc ccttagatgt ggcaacccaa atcctttttt gccggtcctc
    48061 ggctatatca tcaattctaa ccctatcatt tggaagcaat ggaagcctat taaaggaata
    48121 gcctaaagtg gaatttatat aagttgcaag gtttaacccg gcattgcttg taccaacata
    48181 aattttcctg ctggacgatt cgaatatctt gcctacatag ttatcaggaa gtccattttt
    48241 agtggaatac tggtaaatcc tggtagggct aataactgat aaaccctcac cgatggttcc
    48301 tgcccataaa gtaccatcac ttaccctgca aatcccccat attttattgc caattaaacc
    48361 atcgttggta gtaaggcagg taaatgcttc tccttttagg attgctatgc cattattagt
    48421 taaaatccaa atgttatgtt catggtcaac atatagcttt gaggggatat cgggcaagcc
    48481 attcttaaca cctaacgttc ggttaaaact ttcatcttca aggtcaaaag catggattga
    48541 aacctcatca gaccctttaa gctttccgtt acaccaaatc ttactgccaa tacaaacaaa
    48601 ctttaatata tcgatcctgc tggcatacgg gtgatttact tttataggtt tatcatcaac
    48661 tagcctatat agtccatcac tggtagctac aaaaacatcg ccatttgcat tttcactaca
    48721 tgcattaaca taaaccgagg gttcaaacgt taaagggctg aaggattcgt tactcaactt
    48781 gtaaagagtt cccttatgcc ctacatatac cttgtttaaa gtggattgaa aaacaaaatc
    48841 ggttaaaggc ctgtcctgaa agataggact taatgcaacg tttaagttga ccggtagtga
    48901 gtactgaata atatcaaatt tatccttgct aaaagcaacc ctgaacacac ccctattgct
    48961 taaaacccaa accccattgc ttgatttacc aagtgaggct acttcgccaa ttgtatcttt
    49021 gggaattgtt aaaattctat tacccctaac catgtttaat cccaacgatg agcttgccca
    49081 aatattgcct tcggcatcaa cagcaacatc ggttaagcgg gtatagttga gcccgtccct
    49141 tgtagtcagg tatttaaact caaatccatt gtagttacaa agccctcctc cggtagacaa
    49201 ccatactttc ccctgggtat cctgatcaat accagtaacc aaggaatgca gcaaaccagt
    49261 ttttagacca atgatttcaa tattgtatcg ctgtgcaaac gacacaaccg acaggctgag
    49321 tagagataaa actattgtaa aacgcattaa atgataggct taaaatttaa tccattaaat
    49381 ttataaaatt gaagaattta ttcctattac ttacataaaa atctgactaa aaaatagtaa
    49441 ttgctaaatt tgcagcacta aactaaggaa atggaaagag ttgtagtagg cctttcgggt
    49501 ggtgtagatt caagcgttgc tgcttatcta ttaaagcagc aaggctacga ggttattggg
    49561 atgttcatga aaaactggca cgataccacg ggtgtactta agggcgattg cccttttgac
    49621 gatgataaac aatttgctga gatggttgcc cgtaaactta atatcccttt taagtttgtt
    49681 gacctcagcg accagtaccg ccagcgcgta gtagactaca tgttcagcga gtattccaag
    49741 ggtcgcacac caaaccccga tgtactttgc aacagggaaa taaagtttga tgcttttttg
    49801 gaagcagcca taaaacttgg tgcaaagtat gtggcaaccg ggcattactg cagaaaagaa
    49861 actatcacaa aagataacac tactctgtac cgcttacttg ccggagttga tcccaataag
    49921 gatcaaagtt actttctgtg ccagctaaac cagcaacagc tgagtaaatc gctctttccg
    49981 attggtcatt tgcataagcc cgaggttagg aaaattgcag ctgaacttgg cctagcaacg
    50041 gcagagcgga aggattcgca gggaatatgc tttgtaggga aggttgattt accagtgttc
    50101 ctgcaacaaa agcttaaagc taaaaagggg aaaatagttg aaatacctgc cgatttttac
    50161 acccagttgt cgagtattaa tagtaacgat ttagcatcgc tttcaaaacc atacacctat
    50221 aagctttcca acggaaaggt agttggcgag catcagggcg ctcacttttt tactattggt
    50281 cagcggaaag gcttaaatgt tggcggtaag gccgaacccc tatttgttat agcaactgat
    50341 attgagaata acattgttta cgttggtatg gggcaaagcc accaggggct ataccgctcc
    50401 ggactttcca tgaaccccaa tgaggttcat tgggtccgcc ccgatttagc attgtctgca
    50461 gggcaatcgt tgcaggttct ggctcgaata cgataccgcc aacccctgca gcaagccacc
    50521 ctacatcaca ccaataatgc atactacctg atttttgata cccctcaacg aggagtaaca
    50581 gcagggcaat ttgcagcatg gtacattgac gatgagctga ttggttctgg tgtaatagca
    50641 gaatagtggt tgagtatttg acgaaatttg atatatttgt tcaaggatta ccacaaccta
    50701 atagtaaaag ccttgaaccg tcagcttagt ttcctgatag taaatatttt gctgacatta
    50761 agtagttata cttactgtca aagttcgctt cttataaata actttgagaa aaaggactat
    50821 cgggcaggaa atcagaactg gtctatctgc cttggcatgc aaaatcatat tctgtttggt
    50881 aacaataatg gactgctttg ctttgatggt gctagttgga agattaaacc tcatccgaac
    50941 aatacaacaa tacgctcagt tttgacagct aaaaatagaa tttacgcggg aagctttgag
    51001 gactttggct actatgaaat attgaacaat agtattggca attacgtttc gcttgctaag
    51061 ccctttagat cgagtataca caatgaggag ttctggcgta ttgtatcaat caaggatatt
    51121 attttttttc aaagctttgg aacaattttt tacctgtcaa aggatagaat tggtaaaata
    51181 cccctggatt tctcagttct tctgctaatg caagccggtg aaaatatttt tactcaagcc
    51241 attaacggac caatttaccg tgttgataca ctaggcctca aacagattaa agggagtgaa
    51301 attttcaaaa acaccgaggt taaatcggtt gtagtgctta atagcgatac gctttttctg
    51361 acttcaacaa tgggaatttt taggtttgat ggtaacgctt tcactccatg gaaaacctcc
    51421 attgatattc aacttataaa atcaaatcca aacattgccc aaagccaagg aagtaaactt
    51481 gccataggta caatacttga aggtgttttt atttttgata acaaggggaa actattacat
    51541 cacatagact cgaagctaat tcataacaat acaatcctct ccatgcaatt cgactcaatt
    51601 ggaaatcttt ggttaggaaa agacaagggt ataagctacg tggcctttaa ctcaccaatt
    51661 agcacatatg ttaatgaaaa agaaagcttt gcgtgctact ctggagccct ttcacagaat
    51721 aaactattcc ttgcaacaaa ccaaggagta tatacctact ctctcaaccc aagaggcgaa
    51781 tttgagaacc agggtgtgat tgacggaacg caaggtcaat gttggtttac caagcaaatt
    51841 ggtaacaagc tttactttgg tttaaataat ggtacctact catattccaa tggcaatctg
    51901 aacaaagtat gctatatttc aggcggttat aatcttgaac gatttggcat agaacctagt
    51961 ttattcattc aaagcaccta cagaaattta gttacttact catacgagaa tggttcgctc
    52021 agggtaaata aaataatacc cacatactca tcgcctacac gctttttgca gatcgatttt
    52081 caaaactact tgtggctagg acacacagtt acaggaattt atcgtgctca aatcagtaac
    52141 gatctttcag aaattgtgaa aattgatact ataggaataa aaaacaatct gaatatcaac
    52201 acaaacagaa tatataaaat tgaaaacaga attgttgtac caactcaaaa aaatatctta
    52261 agatgggacg atatttccga gcagttcata ccctttaacg aaatcaatta ccagctacag
    52321 ggttttgaaa aatgtaaagc aataatttcg ctccctgcaa accattactg gtttataaaa
    52381 gatgatgaat ggggtttatt tgaaatacgc tacggtgaag ctaaacttaa gtatagaata
    52441 ttgccccaag cctacaatat tgaaatggtt gatggatacg aaaatattgt tagccttaat
    52501 gatagccttc aaattatttg cttagataat ggtttctcca ttcttaactt aaaaatgttc
    52561 gatagaattc ctatcgacat tcaccctcca attattacag aggttgtaat ttggaacggc
    52621 gaacaaaatg taaaaagcca cccccgctta aatagaaata atgaactcac aatcccatgg
    52681 cgaaaaaata acgtttcatt ctcatttact tcaaaggata ttattggtgt taaacacttc
    52741 taccagtata tgcttgatgg tgttgaccgt gagtggagcg agtggacccc aaatacatca
    52801 attgactaca aacgactttc gcccggtacg tacaggttca aggttagaac gctagattca
    52861 aagggtcata ttaccccaaa cgccaccgtt acgataaagg ttttacctcc cctttacgca
    52921 tcatggaagg caattggttt ttacatactt cttggttttg caattacagc ttacacaaga
    52981 gcaaggtata aaaagaaagt gaaaaagcat tacgaagaga aacgtaaact tgaacttgag
    53041 aaaattaaca aggaaaagga agaaaaagaa aagattatca ttaaacttca gaacgaaaag
    53101 ttgcaagccg atattgaaaa taaaaactcg caattagcgt tgagcacaat ggcaattgtc
    53161 cgcaaaaacc aactcttaca ggatattgcc caagaacttg aaggcctaaa gaacgaactg
    53221 gggtaccgaa tcccaaataa gtactacagc aaaataagca ggttaatcac ccaagacatt
    53281 gaaagcgatc acgactggga aatctttgaa aaactattcg atcaggccca tcaaaacttc
    53341 tttagacgat taaaagagaa ttacccatcg cttacaccaa gcgacttgcg gctttgcgca
    53401 taccttcgca tgaatctatc gtcaaaagag attgcacccc tgcttaacat tagtatccgt
    53461 ggggtagagg aacgaaggta ccgattaaga aaacgattag gactcgacac taacgataat
    53521 cttaacgact tcatacttaa gttttagtag ggccaaacta tatttcattt caatctttca
    53581 atagttctaa caaaatattt agggctaact attttctttt tctttaactt ctctaaccct
    53641 atcatattcc atctattccg actcatttat ttatgcttca ggctttccgt tttattagtc
    53701 ctgtttaatt tgttaaatca tggcacttgg gtgattatta gattaatttc ttcaaatcat
    53761 gctttgtaaa attttacaaa acattctgtt gtatttattt gtttaccaat tatttaacat
    53821 tcctttaatt taaagtgatg tagtattgag caacggaatc caggatagat tttcgacccc
    53881 cgtaattaat gatgatgtta aatagtggac tttagctaac ccaaactata attttggttg
    53941 cgcaaacgtt ttaaactaat tgttcaaaaa acaaaagcta tgcacagcct aactcaaacc
    54001 aaaccaaaaa ctttaatacg catagcggga actttattgc ttgttctact tttcagttca
    54061 tcatttgcac agcaaataat taaaggaact gtaaaagcgg gcgatacagg cgaaaaactt
    54121 cccggtgcaa acgttactgt taagggtaca acccgaggta ctattaccga tttggaaggg
    54181 aactaccaaa ttgaagtgaa acccagcgat aagatcttag tgttctcatt tgtaggattt
    54241 gaggatacag aggttgaaat tgcaaatcag aaggtaatag atgttacact aaaattgaag
    54301 tcaacccaac ttcaggaagt tgttgtggtt ggctatggaa cccagaaagc caaggacctt
    54361 actgccccaa ttgtgacagt taaaggtaac gagctatcaa aacaggttgc atcgaaccca
    54421 atgaatgcct tgcaaggcaa agtctccggt gttcaaatta ttaacagcgg tgaaccgggt
    54481 agcggagcag cagtaaaaat aaggggagtt ggctcaattg gcgattatgc aaaacccctt
    54541 tacgtggtgg atggagtttt tgttgacaac atcgactttt taagctcgag cgatgtggaa
    54601 gatcttacag tgctcaaaga tgcctcggcc gctgctattt acggtgttag agccgcaaac
    54661 ggtgtaatta ttgttaccac caggaaaggc aaaacaggta tacctactgt aagctacgat
    54721 agctacgttg gattccaagt tccagtaaac atccttaaaa tggcttcaaa ggatcaatat
    54781 attactctcc ttaacgaggc aaatgagaat gtcccgggtt atgttcccaa aaatcctgcc
    54841 gattacccta ctagcaccga ttggtatcag gaacttgttc gcccagctgg tatgaataat
    54901 cataacctgg acatttctgg atcaaccgat aagacttcat actcaattgg gggcaactat
    54961 atataccaga atggtattat ggatgccaag aacaattacc agcgttataa catcagaggc
    55021 agacttgacc agcaagtaaa taaaaacctt aaaataggca ttaatactat tatatccaat
    55081 tactccaggt tcaacccaaa tacgtatgct ttttttggtg catatgttaa cccacctgtt
    55141 tactcagttt atgacccaac aaacagcgat gcctaccctg taaagtttgg agctccgcaa
    55201 aaatacggat ttggtaacga gtacgggaat cctgttgcgt ctgcctacta taccgataac
    55261 tttgaaaaag gacataaaga ggttttcagt atatatgctg aactcgatat tattaaagat
    55321 aaactaacct accgtgcagc ctacaatcag gatcaggata attggaacca acgcagctat
    55381 acccctcagt tctatgttgg tggctcgcag ggagtaacta agtcaagcct gacaaaaaca
    55441 tttggaggtg cctccaaaca aattattgat aacctgttaa cctataagaa tagtgctgga
    55501 aaaacatcat actccatact cctagggcat tcgatcagaa tggagaaatg ggaaaccctt
    55561 tcaggctttg ccaatagcgt tcccggatat gatgaacaat cgaaatacct tataaatggc
    55621 tcattccgcg accgtaatgc atacgacggg gcagcaaggt acaacggact gtcatttttt
    55681 actcgaggta cactgaacca caatgataaa tatcttgcaa cccttacatt ccgtgccgat
    55741 gcaagttcaa aatatcaaca aaaatggggt tattttccct cgttgggtat tgcatggaat
    55801 atcacccagg aaaactttag tagaattaat gaaaagtttg ccaacctgaa gttacgctta
    55861 agctggggaa tgcttggtaa tgacaatgta cccgccaact cctcgatgat tcttggtcaa
    55921 acaggtgttg gaagttcagg agtgtttggc gataatttgg tggatggtat gggtgctcaa
    55981 actgttctgc agaattacct gaagtgggaa acagttacag agtttgatat gggttttgac
    56041 tttacactta aaaatcagaa agtatcgggt gaactcgatt attaccgacg tgttaccaac
    56101 aatgtagtgt tttatgctcc cattgccacc ggaggtggta ttgctgagct tttaggtaat
    56161 aatggaaaag ttttgaacac cggtgtggag ttctcgctga actacaaaaa cgagatatca
    56221 aaggacctta attacaacat ttcgttcaac gctacattca accataacga ggttcttgaa
    56281 cttaatggta gggagtacat ccccagtggt tatgtaaggg ggaactatac aacccgaact
    56341 gctgttgggc accccatagg ctcattctac gggtatgaga ttgacggtgt atacaagagt
    56401 gaacttgaag ctttacgcga cccagtaagt caagccatta aggataaagg atatttcaag
    56461 tataaggatc aaaacggcga taaggttgtg gatgcaaatg ataaagttta cctgggtagt
    56521 gcaatacctt ggctaattac ggggtttgat tttggtttaa actacaaaaa atttgatgta
    56581 agcctttcca ttaccggaca gtacggcaac aaaattttga atgccaaaag gatgaacagg
    56641 gatgtatttt ccgatggaaa ctacgatgaa gatttttaca ataaccgatg gactccctca
    56701 aataaatcga ccgattaccc atcggctgaa gcctataact cttcatttat tcagcaagct
    56761 aacgatttct ttgttgaaaa tggtttttac attcggattc agaatgtgca aataggctac
    56821 acaaccgata aaattcctta cgtacccaaa tttcgggtat tcttcacagc ccaacgccca
    56881 tacacattct ttacatacaa aggttttacc cctgaggttg gaggatcgcc tatcagtagc
    56941 ggcatcgact tcaatgtcta ccccatgcaa gccatttata cactgggtat taagctgata
    57001 ttttaactca aaatctaagc gccatgaaaa cattaaaata catcataata actcttgcat
    57061 cggttctgtt actttggaac tgtgaagatt ttctcgactt tcggcaggag ggaaccttac
    57121 ccacaaccgg aattgattac accaagtccg aaaatatatt tctttcagta agcgctgcct
    57181 acgccaagtt gagaaactgg ggagcacatg tttttccgta cgttggggct tttgaaattg
    57241 cctcggacaa tgccgataaa ggtagtacac ctgaagataa cccagaaatg cgcgacattg
    57301 acaacctgaa ctttcagcct aacaacggtc ttataaatgc cttgtggact ggatactacg
    57361 atatagttag cggtgccaac tacgccattc atcaaatgcc ccttttcgtt caaaacttac
    57421 agaataaatc ggatcgcgat tatgccattc aatgccaagg tgaggctaaa gccattaggg
    57481 cttacgcata ctttaacctg acccgtttat ttgggcgagt acctatagta gatacggttc
    57541 tttcctctga acagttagcc tcacttagtc aggcgagtac aagtgaatta tacgctttca
    57601 ttgagaaaga tctggaagag gccattgctg ttctacccga tggttacacc aaggaatggg
    57661 gtggccgaat atccaaatac actgcaatgg caataaaggc taaagttcac ctataccaat
    57721 cggaatggga ctccgtagcg tcacttaccg ataaaattat tgcatcgggc aggttcagac
    57781 ttctcgataa cttccgcgaa gtatttagca tggatggaga gaattcaaag gaatcgcttt
    57841 ttgaaattca aagttctaca cttggaaagt catcgggcga tgctgcttat cttgagtatg
    57901 ggtatgtaca ggggccgcgc ggaaactcac ctgccaatat gcaaggctgg ggtttctgta
    57961 caccaagtca aaacctaatc gatttttata cttcgcgtgg tgaaataata agacccgcaa
    58021 ctacactgct ttacagggga actaaaacac cggaaggcga cagcattaaa atgagttgcg
    58081 ccaaccctgt ttacaacgga aaggtatata caccctcatc gcaaaataga tgggtgtaca
    58141 atgggtatgg ttttgaccat aacatcaggg taatcaggta ttccgatatc cttttgatgt
    58201 atgccgaggc attggtgcaa ggcgctaccg ttcccttaac ctgtggctta agtgccgata
    58261 atgctattaa catggttcga aacagagctg gtttacccag tatcagcgga gctactctac
    58321 aggacatttg ggacgaacgc cgtgccgaac tggccatgga ggaggacagg tattttgatc
    58381 tggtgcggac aaatcaagca gcaaccgcgc ttgcatcgaa gggatttgtg gtaggaaagc
    58441 atgagcatta cccaatccct gctgcccaat tgcaactgaa tccgaattta actcaaaata
    58501 gcggatatta atttcatttt taaataaacc tatgtctaac taaattttaa gtcctatgaa
    58561 gaacaaattc agaatttttg gcgcgcttct ttttggcgct gtagtaatgg gtggattgct
    58621 attcaccgca tgcagtaaag atgatgataa caatgacaat gggaaggttg atccaagtac
    58681 cattgcaacg gcaaacctgg ttgcttacat gccatttgac gacaatgccc ttgataaagt
    58741 tggtaacctc acaccatcgc aacaacctaa tgttacttat gttgtaggac gtagaggtaa
    58801 ggcttaccag ggtgcagatg gcgcatatct acgctacaat ctacctgaca acagcaaact
    58861 taaaacccta aagtcgtaca ctgttgccat gtggattaaa tcccctaaag tagatggtac
    58921 acctgtacct aaggttttcg aaatcggaaa atccgatgat ttattctggg gaaacctcac
    58981 aatcatgatt gagaggctta ctgctcctgg tgccgattca ctattcttta aactacactt
    59041 taaaaaagta ggtgttccct gggaaggtca atggattgaa gcaaagaaat cgattttcca
    59101 ggtaaataaa tggatgcacc ttgtgttcca gtacgatgct actacctcca agtttatgat
    59161 ttacaaggat ggtgcaaaag ttgaattccc tgcagcagtt gaaaatcgct acgccaacga
    59221 tcagcaggaa ccccttggcg atctgaactt tgttaacgcc gacgtgctta acattggcgc
    59281 atggagacca aaaaccgatt ctcaagccac tgatgaatgg atggggtggt tcatgggcaa
    59341 cctggatgaa ctccgtgttt acgataaagc actatcagct acagaagtta aggctttgta
    59401 cgatgccgaa gtatcacaaa ttactgaata accgaatttt tagggtgggg gaaacacccc
    59461 accctatttt ttattaaacc ctatgaagta cgttttttcg ctaatcgcac taactggaat
    59521 tttaattacc gtaacctttt gcaaagaaga ggaaacggta aaacccattg gaaatataat
    59581 ggtcgactca attaccattg acgggattaa agtttatgac aatggtcaaa tcaaccatat
    59641 tggttttaaa ccaaacataa aagtctattt cgataaacct gttgatacaa caaaaatcga
    59701 cacctacaat atttactttt ctggagatac atacaataat tacacccatc aattcaatac
    59761 tgattcaaaa gttttaagta ttatacccaa cgaaaatctg aaacctctca cgctttatcg
    59821 gttcacggta tcgcagggca aaaacttggg tggcaatatc ataaatgggt atacagccaa
    59881 gttttttacc aagctcgatt caaccgataa gtttcagcaa atttcggacg atagcctatt
    59941 aacgttaata caacatcgaa cctttaagta cttttgggat tttgcccacc caacctcagg
    60001 tttagcaagg gaaaggaata catcgggcga tatagttaca attggaggta gtggttttgg
    60061 cataatggca atcattgttg gcattgaacg aggtttcata accagagccg aaggcataag
    60121 ccgtttctca accattgtaa atttccttaa agatgccgat aggtttcatg gcgcatgggg
    60181 ccattggtat aacggtgtta caggtaaagt atatcctttc agtacaaacg ataatggagg
    60241 cgacttggtt gaaaccgcgt ttctggttca ggggcttatc acactccggc agtaccttaa
    60301 ccctaatgag agttctgaaa acaacttaat aaaccaaata aatgagttga ttaatggagt
    60361 agaatggaca tggtacacta aggatggcga ggatgttctt tactggcact ggtcgcctac
    60421 cgttggctgg gcaataaata tgcaaattag aggctacaat gaggcattaa tcgtttatgt
    60481 attagccgct tcttcaacta cctaccccat taacccttcg gtttaccatt ccggttgggc
    60541 aaggaatgga gctattacca acgggaagac tttttatgga atatccctac cggttggttt
    60601 tgactacggg gggccccttt tctttgccca ttactcattt atgggccttg accccagaaa
    60661 cttaagtgat aactatgcaa actactggca gcaaaacgta aaccacaccc ttattaaccg
    60721 tcaacattgc attgttaatc caggacgata ccccttgtac agcgctgatt gctgggggct
    60781 aacagcatcg gacgacaata caggctatgg tgtgcatgag cccacccgcg atattggagt
    60841 tattagccca actgctgctc tatcgtccat gccttatacc cctgaggaat cgaaaaaagt
    60901 ggcacgcttc ctctactata aacttggaaa caaggtttgg ggtgagtacg gcttttacga
    60961 tgcatttaat gtaactgcaa attggtgggg tacaagctat ttggcaattg accagggacc
    61021 aatagtagta atgattgaga actatcgtag cggtttatgc tggaatcttt tcatgtcggc
    61081 acccgaggtt aagcaaggac tttcccgtct tggtttcaat actagcaggt aacctaaaaa
    61141 acgcatggct atggctatca atggtaaaat tggaatgttt tggaaggtat caatggtgct
    61201 taccttccta atgctctcat gctcaaaaac taataatatc agctttccat caaagggcaa
    61261 aataaattac tccttaactg ccgaggaaga aaaaatgctc gattcaattc agtataagac
    61321 ttttcagtac tttatgaatg agcatcaccc acattggggt gctgtaaaag acagagcagc
    61381 caaatggtcg ccaattagca ttgccgcaac aggttttgct attccttgct ttgcaatagg
    61441 tgctgagcgt aaatggatta gtcgcgatga ggcagctcaa ataaccctaa atatccttaa
    61501 attctttaaa aattctgagc aaagtcctga tcctctttct acgggctata aaggctttta
    61561 ctaccatttc ctaaacatgg aaactggaaa aagggaatgg aagtgtgagt tatcatcggt
    61621 agatacaggc ctactgctca tggggataat ttttgcccgc aactactata atgcaaacaa
    61681 tagcgtggaa cgtcagatcc gccaaattgc tgacttttta ataaaaaggg ttgactggga
    61741 tttcatgcaa atgccacctc aagggcaata cgcttacacc atttcaatgg gatggtatcc
    61801 ggaatcgagt atacacaata tgggctggaa agggtataac gaagcgctat tcctatacat
    61861 actggcggca ggatcaggaa tgaaaaatat tgaaaaggca tacaattcat ggctcgaatc
    61921 ctacaactgg tacactcctt acaaggattt ttcacatgtt gcattcccgc cactttttgg
    61981 ccatcaattt tcacatgctt ttatcgattt tcgtggtata caagactcat acatgagcaa
    62041 aaaaggaatc gattacttca ttaactcccg tattgccact tacgctcagc gagagtattg
    62101 catcgataat ccccatgggt gggttggtta cgattcactt tgctggggaa taactgcatg
    62161 cgatgggcca accgataagt ataattttga caataaagtt tttctcggat acgccggcag
    62221 gggggccagc ggaagggaac ttaactactt tgatgatggt actatagccc cctatgggca
    62281 tctatcatcg ctaccttttg ctcctgaaat tgtgctccca acaactaaag caatgttgaa
    62341 gcgctatggc gataaaatat ggggtaaata tggattttac gactccttta atccaactgc
    62401 taaatgggtt gataacgact ttattggaat tgatcaaggc ccaatgctta ttatgattga
    62461 aaacttcaga acaggcatgg tttggaacta tataatgccc gatccaataa ttcagtcagg
    62521 gttaaagaaa cttggttttc agaatataaa ttgattgttt tctgaaatta cgaatcgtca
    62581 gtttttttct tattaaaaca ctcttggtat gaaccatcac ttaaaggcaa caattactgc
    62641 tgctttgcta tcagttttaa catttcacat cggagtaaag gctcaaacag taactctcga
    62701 tcagttcgaa tcaactgaag gctggaacta tattaagtcg gatggggtaa atattaccgc
    62761 tcaaaccgat aatggtcttt ccggcaaatc catttgcata acatataact ttgttaaggg
    62821 tacaggttac tgtggtattc aaaagctttt tcccattaat ttgccagaga actatgagat
    62881 aaccttttac ataaaagccg aatcgccctc aaataatttt gaaattaagt ttattgacag
    62941 tactggtaac aatgtttggt gggtaaacaa ccgcaattac aatttcccta caagctggca
    63001 aaaggttaga gtaaaacctc gccatatcag ttttgcttgg gggccaacca ccgatacacg
    63061 ttttagaaga attgatagaa tagagtttac cgtggcatcg ttcgttggag gaagtggaaa
    63121 aatatggatt gacagtctaa cctttaaagc cctaccccca atttccaatg agtaccctgc
    63181 cccagttgcc tatgaaattt cgggtacaca atcaaaacca attgccgaaa tacttgacca
    63241 gcagccccac agcgtttgga ttgccaaaaa caaaagtatt caggagatac tgattgattt
    63301 taaagcgctg cgtgagattg gcgggttaaa aattgattgg atgcaaggca aacaagccca
    63361 aaaattcgat gtattgttat cctatgattc cataaattgg gaaaaaactt ttactgtcaa
    63421 tttaaatatt aacagcacaa gctacatccg cctgcctgag tttgaaaccc gattcttaaa
    63481 actaaaacta agccaaagta ataattccaa gggtttagga attaaggata ttgaaatcat
    63541 aaaggttgag gaatcgaaca cacttaacaa cttttttagg tacatagcaa agaattcgcc
    63601 aaagggctat taccctcggt actttcttga tcaggcttcc tactggacaa tttcaggtgt
    63661 aagtggcgat acaaaggagg cactgattaa cgaagatggc atggttgaag ttgaaaaagc
    63721 aagtttttcc attgagcccg ttgtaaaggt tggcaataaa atttatgatt ggtcgaatgt
    63781 aaaaacctct caatcgctta cctattttat tgatggtaaa acctatgatt acacacctac
    63841 ggttaattgg cataccgatg atattgactt acaggtaggc attacctcct acggaaatgc
    63901 aaataactca tcaaaactac tgattggtta caccctaaaa aacaatacca acaaaaacca
    63961 aacaatagat ttttacctgc taataagacc ctttcaggtt aacccatact accagacctt
    64021 aaaccttgcc ggtggagttg gaacaatcaa aaaaatatca ttagacgcat caaaagatag
    64081 ggttgaagtt gataacacct cactatactt tacccaaaag tttgagtcgt tctttgccgg
    64141 ttcattctac agcgctaatc ccaccgaatt aatacggagt aaccacaaag aagtagtaat
    64201 ggcagaaact gacaatttgg gtttaaatgg tggatttgtt aaatacacca taaagctaaa
    64261 ggccggacaa tcccatactt tatttgctat tgctccatat cattcaaaaa atatcaatga
    64321 ctcagaatta aaaacaaaaa atatcgtcga tacaattgca aaagtgtcgg actactggcg
    64381 cggcaaaacc gaatacgtaa aattcaattt acccagcact gccgaacatt tattaaaaac
    64441 ctaccgtgcc aacctaatgt atattttaat taaccgcgat attcatggca tacaacccgg
    64501 ttcccgttca tatgagagaa gttggatgcg tgatggttcg cttacttcat cggcattact
    64561 gaaatcgggc attactgatg aagttaagga atttattgag tggtatgctc aaaatcttta
    64621 tgaaaacggc aaggttccct gtgttgtaga ctttagaggt cccgaccctg ttgcagagca
    64681 tgacagtcac ggtcaattta tctacttaat acatgaatac ttcagtttta cacacgatac
    64741 cgcatttctg cgaaagatga atccttatat cctcagaaca ataggttaca tcgaatcgtt
    64801 gatagccgaa cgatccactg attatttcag gtatggaaac gatagtctga gagcctttta
    64861 tggtttagtt cccgaatcaa ttagccacga gggatactca gcaaaaccta tgcactcata
    64921 ctgggataat ttcttcatcc ttaaagggct caaggatgcc tgcgatattc aaatcgcttt
    64981 gggaaatcat gaaaaatata cgcgcattaa gcaagtaacc gatactttca ggaaaaacct
    65041 atataaatcg ataaacctga ccattaagaa ccataatatt aactatatac caggatgtgc
    65101 cgagctggga gattttgatg ccacatcaac aacaattgca cttactcctt gcaatgagtt
    65161 cgaaaatctt cctaaacctg aaatttacaa tacctttgaa agatactata catactttag
    65221 ccagagaaaa acgggccaaa taaactgggt caactttaca ccctatgaga acagaattat
    65281 tggctcgttc ataatgctca atcaacccga aagagcccat gagttaattg attacttcct
    65341 aagccagcaa cggccccaag gatggtatca ctgggctgaa gtggtttgga atgattatag
    65401 aactccagct tacataggcg atatgcctca cacctgggta ggtagcgact tcattaatgc
    65461 cttcagaaat atttttgttt acgaagatat tgaccacaac accttatgta ttggctcagc
    65521 tcttaaacag gattggattg acagccctca aggaatttcg gttcaaaacc ttccaacata
    65581 ctacgggaac ttatcttact ctattacaaa aggaaacgac cactacaaaa taaaaattgg
    65641 cggaaatatt agagttccag ccaatggaat ctgtatcaga aactttaatg gttctaagct
    65701 tcccaaaggg gtagaagtga atggtactaa gcttaatacc tacaacgtaa atgagatacg
    65761 agttaatgaa atcccctgcg aaataaaaat ttactactaa taacttaatg taatggaaaa
    65821 caataattta caggcaaaaa gtgaatatag gataaagata tcacagctat tagcttatgg
    65881 ggctggcggc ataataccca ttgccctttt taacattgct ggcatccttg ttgggctcat
    65941 ggggaacata agcttaggct taagcgcttt ttggttggga acaatactaa ttataccacg
    66001 actttgggat gcaatagccg accctattat tgggcatcta tcggataata ctcgcactcg
    66061 ctggggccgc cgacgaccct ttttactggt aggaggaata ctggtagcat tgttctttgt
    66121 ggccatgtgg tggataccca aaggcgaaac catacgaatt tggttcccct ctgagtcggg
    66181 ctatcagtgg tttcagctat cctacatact tgtaacactt ctgcttttct acacctcgtg
    66241 cgcaatattt gaaatccctc atggagcatt agggatggaa atgaccgttg acccccatga
    66301 gcgtacacgg ctgtttagcg caaaaagttt tgtgggaaat ctttttgcca tgagcacccc
    66361 atggctcttt gtgctcgcca acctggaaac atttaaaggc cccggaggga acgaagccga
    66421 tggaatgcgc tatgtttcat taatgattgc agctttgctt atacccctct ccttttggtg
    66481 gactgcaaaa ctacgagaac ctagctttgt gagggtgcag aagcaggaga aaaaaccttt
    66541 ctggaccgac atgaagcata ccacaaaaaa caggaatttt attaggctta ctcttgccat
    66601 ttttaccctt gcaaccggtt tcaactttgt aacgctgttg ggctcataca tacctatttt
    66661 ttacgttttt tcaggcgata aggtagctgg agcatatctg cttggcatta atggaaccat
    66721 atgggctgtt acagggcttt tagcagtttt tccattgaac tggataagcc ctaaactcgg
    66781 caaacgcaat acattaatcc tatccattgc attaatggcc accgcacagc tttcaaaaat
    66841 agtttgttat aaccctgaat acccgtacct gataattatt ccaactattc tgctctcagc
    66901 aggaatgcta ttcttcttca cccttggctc ctcaatggta ggtgatattt gcgatgagga
    66961 tgacttaaat actgggcata gaaaggaagg aagcttttac tcggttttct ggtggtttat
    67021 taaaatggga tttgccgtgg ccagctttgt tgccggaatc cttattgagt tcactcagtt
    67081 cgaccagaac caggtaacaa aagttgacta ccttgaaggt agcataaagg aaatcaaagt
    67141 aaatctaacc gataaaaacg ttttgaacta caccgattac ataaaaaaaa taaaagatat
    67201 taacactgat atacttggta atgcaattaa atacgatgct tttttggaaa gagtaaatca
    67261 gcacgaaaat cctcaaaccg acagcctaaa aattcaagcc aaagtagctt tacgacagaa
    67321 cctaagcatt actatcgaca gcctaaaata ttgggattct gttctaaata catcgagtcc
    67381 cgactctgtc aacctatcaa tcattgcaca aaaattaaat aaacaaagaa tgaactttaa
    67441 tttaattaaa gctcaaaaca atgtttacga tctaatgaac cattttgaat caataattgc
    67501 cggtaaaggt gacggaaacc actataaaga gttactaagg gacattgcac aaataaattg
    67561 cgtgctccaa gagtataaca tttatgatat cgattccgtc aacatccata agctggacga
    67621 aataacccaa aaaattcctt ccctaaaacg tcagagcccg ttcactttac ttatgatgag
    67681 agtagttgaa attggattgc cagttattat gagtatactt tcgttgatat tcatatttag
    67741 atataacctt accgaaaagc ggcttcaaga aataaagcaa acattaacaa cccgaaacaa
    67801 actacagaac cagtaaaaaa tagaaatact atgaacaagt ttggaattga aggcggatat
    67861 ttcagtttga atggtagaaa agtatttctg aactccggtg aaattcatta tttcagaatt
    67921 aaacgtgagc tatgggaaga acatctgaaa aaagccaaag aggcagggct aaaaaccatt
    67981 agcagctatg ttccttgggt ttggcacgag gttaacgaag gtgagtttga ctttagtgga
    68041 acaactcaac ctgaacgcga tcttatagga tggatagagc tttgccaaaa acatggtttt
    68101 acctgtattg ttaagcctgg tccttttata cttgctgaat acagaggcgc tggtttgccc
    68161 gattggtttc tggaaaggta tggcgacact attaaaatgc ataacagcaa aggcgaggcc
    68221 gttctgagcg atggcgttag cctgtttagc cccgcatttc tttccaaagt agaacaatgg
    68281 tataaccatg taatgcccgt tatttgccag aaacagatac aaaatggggg acctataata
    68341 atggtgcagg tttgcaacga aattggagtt ttctcatggc ttgcccacca agccgattac
    68401 aatgaacagg taaaagagca atttatcagt ttcatcaaaa caaggtattc aacgattgat
    68461 gagttgaaca ccctttggga aactaattac acctcgtttc aggaaattga gttacccccc
    68521 gatgggaata ccccttataa atccaaaggc gatcgtagcc gcgattacga atggcactgt
    68581 ttctggagaa agtattacgc taactactta cgattactat ccagttggat tcgtaagcac
    68641 aatatttcaa ttcccttata ccataacctt cccggatgga tttacggtca tgggtacgag
    68701 ttccccctca atattaccat gtacgaggac ctatataagg ataaaagtga gttgatattt
    68761 ggagttgacc acattcctga atttgtctcg tataggaacc tccacgacga tagaatcatc
    68821 aacgacattg ttagtgcaat gcagggtgaa aaacctctgt ttgccgctga gtttcagagt
    68881 ggatcaaggg aataccatgt ggttaccaat ccgcgtgaaa tggaactctt ttacaaggca
    68941 agtattgcca atggcctaaa aggatggaat tactacatgt tttcacaggg taaaaaccca
    69001 cccgcaaagg gatattccgg tgatactttt tactggttta cacccttaga taaccaagct
    69061 aaaccatcga gtgcattccc tgttgttcag aaaatgagta ggtttattga aacccatgaa
    69121 gaactcatcc taaccactaa aagaaaagcc gaggtatgtg tccttttcta ctcaccatac
    69181 tacgctactg agctagagcg tcctgaagtc ggagcttcag aactaaaatt tgttccagct
    69241 cggatccgtc ggtcggcata tttcgatgga ttactcaaag ccctccaggt tctcaatgtt
    69301 gactacgata tggttgacct agccagctca tcggtgaacg atttgttggc ctataagcag
    69361 gtttggctct ttagtaccga tgagatgaat gctcccgatc agcaaaccat tgcaagtttt
    69421 gtgactaatg gtggtaattt ggttattttc ccctacttac ccgataggga gatgaaccaa
    69481 aagccttgca cagtactcag ggatactttt aaaataaaat catcacaacc ggaaataatt
    69541 gattccccct taatcaaact ttataatttg accgatatta aatgctcaaa cccactgatt
    69601 gcctatacaa cagaggctat agatggtgct actcctatag cctttaccct gaggggaagt
    69661 atttgcggat ttgaaaagaa tgttggaaag ggcaaagtag ttcacctggg tacctggcta
    69721 ggatttgata ccgaaggcca tttgcaagcc tacacaacca tactagagcg ttcaaaagcc
    69781 caattggcaa acgcatcgag cactaacaat tttattaccg tacgtcagaa gtttaatgat
    69841 acctctgaat cgcttttatt tgttggaaac tattacaacg aaaatcagag cggaaaaatt
    69901 acctataagc atcccaatac aaacgaaagg gttcaactcc cactaatcga tggagaaatt
    69961 gagattccac cgctttacgc ctttatttca ccaattagta aagaaattgg taaaggcatt
    70021 aatatacttc atactacctc cgatgttatt gggattaact caaacaaaaa tatcattact
    70081 atacacctta cgggcgaccc aggattgatt ggtgaaatgg ttcttgaagg gataaacatc
    70141 accaaaatta agtcggccga aatctccgaa aaacccatta tgctcatttg gcataacaat
    70201 cggatagtat tgaactactt acacagcaac aaggaaattt tgttaacact aaaaatgctt
    70261 gacaaatgaa gctgaaaaac aattcaggtt tagaatttga atttcacagt aatggcggaa
    70321 taaagcgtat ttcatctaat ggtattcgta taggtttacg cgataccaac ccattctcaa
    70381 tgtttgggac aagtatattt cttagagaaa gaaatacccc caagaaatac ataatgcttt
    70441 tcgggacagg cagtaattct gtttttactt ttgaggataa ctgttacttt gcaaaaggga
    70501 agtggaacgg tattgaatac ttaatcagcc ttcagcttca taacacagat agttgctggc
    70561 tatggtcaat tgaattaacc aatgggaatt cttcaccttc caattacgac ataataataa
    70621 ttcaggatgt tggcttaaag cagctatcag ttggcccagt gaatgaatac tacgtaagtc
    70681 aatacactga aaggctaatc ctggatgatc ctcagtatgg tgctgtggtt tgctgccggc
    70741 aaaacatgaa agagcctacc ggtaaccctt ggttaatggc ggcatgtaaa aatggtgcag
    70801 cctcggccac taccgatggg ctttatgttt acggcaactc ataccgaaaa actaaaatcc
    70861 cccttgcttt agaactcgat acattgcccg gcgaatgtgc tgctgaatcg tcagttattg
    70921 ctatccaatc aataccattc gatttcagca gtggtaataa ccaacaaatc agtttcgtct
    70981 ttaggttttt gcccgatcat ccctcagcaa cctcatcaac cgatttagcc attctaccta
    71041 acctttttga acaattcaaa agcaaatacc ctaccacaag caattgggaa aagggaaagc
    71101 ataatctttt tattagctct gaatttttcc catcccagga tttgagtgaa aatgaaatta
    71161 accattttta tagttctgag aaacgattcc ctgactttag taacggtaaa ttgctttctt
    71221 ttttttatgg caagggcaag catgttgtac ttggagaaaa agatattctg gcctatcgtc
    71281 ctcacgggca cattctccag tccaatatca acctaaatca ggatgataat attgtttccc
    71341 ttacaacatg gtcgtttggt gtttttaatt ctcatctaaa acaaggaaac actaatttca
    71401 atacactact ttcaataaat acaagtcaat tcaaccttat acccgaaaca ggtcaaagaa
    71461 ttttcgtaga aatcaacaat aggtattacc agctaggtgt accatcggcc tacgaaatgg
    71521 gattgaatta tgctcgatgg atttataagc tcgatagtaa tatttttcaa gtaacaacat
    71581 gggcttcacc taatacacca gttgtaaatt ttgattttaa ggtaatatct ggccatccag
    71641 taaacattat cattaccaat aacctcgatg agctgaatgg ttggcaagta aaatcgtcag
    71701 gctttccgaa cggtttaacc tttatcccta gcccagaaag tcttctttct aaaaattacc
    71761 ctaaagccca ataccgtttg gttataaaca aatcggattg tgctaattta aaatttgata
    71821 acggcaattg tattgatatt gaaagcagct atgccaattc gtttgtcgtt atcaaatccg
    71881 attcaacaaa acagttaagc ataagtttta tttgtgagct tcaaaacctt tttgaccagt
    71941 caaccatcaa caaccccgac caacagttcc aggcagattt agaagatgct ctggcattta
    72001 gagaaaaatc gggattagaa ataacccttt caggaaattc cgataaaatt gaaatcatta
    72061 acgagattat accctggttt gagtcaaacg ctctaatcca ttacctcact ccctatgggt
    72121 tagaacaatt tggaggtgca gcatggggta cacgcgatgt ttcccagggt cctgttgaaa
    72181 tgttgttaag tttaggtaag tacaatgagg taaaaaaaat tcttcaaaca atcttttcca
    72241 accagaaccc cgatgggtgg tggccacaat ggtggatgtt tgataggtat aaaaccatca
    72301 gggctcacga ggcccatggc gatattgcct actggtgtat tatagccctc tccaactaca
    72361 tcataaatag cggagatgtt gatattctga actcaccttt accctactac accgaaaata
    72421 atggtgacag cagggaggtt actccactaa ttgaacatgt tgaacggtta attgatagga
    72481 ttgttaaatc gttcattcct ggcactgcac tagtgccatt tgggggtggc gattggaacg
    72541 attcgcttca accggttaac agccaattgg cacaacgttt ggtatcgagc tggacagttg
    72601 aaatgaatta tcaggccttt aagctgttcg aaacagtcta taggttaatg gggttaaccg
    72661 ataaagccga agcattaggt gaaataagta ctaaaattaa aacagacttt aataaatatt
    72721 tagttaccaa tggtgttgta gccgggtacg gacatttaga aaacgataac acttttacac
    72781 tccttcttca tccctccgat agtaaaacag gagttagtta tagcatcctc ccaatgaaca
    72841 ggggtattct aagcgggatt tttgatttaa atcaggcttt caatcaccaa aaaataataa
    72901 caaatcacct tttaggcccc gatggtgccc gacttatgga taagcccctt aagtatagcg
    72961 gaggtttaca aaaaattttc caacgtgccg aaagcagtac attttttgga agggaaattg
    73021 gcttaatgta cactcacgaa catattagat atgcggaaac tcttgccgta actggcaaag
    73081 ccgaagaact tattcgtgcc attctgcaag ccgtaccaat tagttacact aacatagtaa
    73141 aacagggcga tattcgacaa tcaaactgct attacagcag ctctgatgtg ctatttaaaa
    73201 ataggtacga ggccgataag ctataccccg aggttattaa aggaaacatc aaattacatg
    73261 gtgggtggcg agtttactcc agtggacccg gcattttcat aggtattatc ttgggcaggt
    73321 tactcggatt ccgattcagc aatgagttcg ttgttattga cccagttcta ccaaaagaat
    73381 ttgatggctt aagggtaaac tttagcttta gtaacatacc atttagtgtt acctaccaca
    73441 ttaccaaatc aacctttagt cccctctctc ttaaagtaaa tcatgtagaa ccgcccttta
    73501 cctacgagga aaacccatac cgaaaaggcg gaataagagt tgaaagaaac ttattttttg
    73561 gtttgctgaa cagcaaggaa aacaggatcg agatcgtact ttagaaaaat attgtttaag
    73621 acaagggatt taccataacc cccattcacc tgcctctgga cgtgtacggc atacttttcg
    73681 ctacattcgc tcgcgcgcgt ttttaaacgc gtgcggcata cgccactcat tgctggcgcg
    73741 gacttgtagt ccgtgacaaa tacatactac acttttactt attcgagctt tgcctcattg
    73801 aaaccacctt ataaaataac cgcacggatt ggctttgccg aactacaaac gcacggatta
    73861 caaccgaccg aagggagctc accgaaggta aatccgcgcg agcgggattg caaatccgcg
    73921 cgagcggcct ttgctgaact ttgcattatg aaataaccgc acggattggc tttgccgaac
    73981 taaaccgcac ggattacaac cgaccgaagg gagctcaccg aaggtaaatc cgcgcgagcg
    74041 gggggaatag taggcgataa catcagtatc atgctggcag ccgcagcatt taacttcaaa
    74101 agggttatga acaggtggaa gaaaaatatt taccatttct tccaaacact atttttcttt
    74161 ttacatgcac tcttttcccc tttccatgtt tttttattct caagaaaaaa ttttaaactg
    74221 actttttaag ggacgactag ttaaggaaca gcgaaccggc actcctgagg agtttgcgac
    74281 caaactagga ataagccgta ctaggcttta cgaaatgatt gaggaactaa gatcgcgagg
    74341 tgcacccatt gtgtactcca agtcacgtga aacttttttc tatgaataca cctttgacat
    74401 ttcattaagt ttttccaaga aacccctaag ccgaaaagaa ttagtagaaa aaaatggggg
    74461 tgtttcactt ccgttcttgt tttccgtacg gaacactcct aattttgcaa ctgtaacgtt
    74521 acactgctaa cctcggcagg ataaagaaag agggtttgat tatttaacaa taaaaagtat
    74581 ttttgctatg gttaattttg aatcattctc tgttcacgaa atgaatacaa gtgaaatgac
    74641 tgatatcaac ggtggtttta gttgggagga ttatttaatt ggcaagggaa ttgatgctgc
    74701 tctagagtgt atgaaagaat tatttatagc atatgtcaac tactcagcta gtacaggtgg
    74761 aaaatatgtt atacatcatg cacaatagtt attaataggg ttataaatat tataacccta
    74821 ttttttgtaa tttttttaat atgattaaac catataaatt aaaatacagt atccaatcag
    74881 ttaatccttt attggttatt attatcacga caataacttt attattaatt gaatcattct
    74941 atcactattt attcttagca tttgacattc accatacaag caacctgcca aagctaccag
    75001 ttgaaaccga tcctgagtct catgtcaaaa ttgctattcg tcttgttaag gtatgtatta
    75061 ttgctccttt agtagaaact tttattttcc aatacttgct atttgaattg ttctggcgca
    75121 aattacgttg gagcttgaat gtgttttacg gtttgagtgc ctttaccttt ggactctcgc
    75181 acttctattc aattccaaca ataattgtaa catttacagt ttgcttagtg tttaattatg
    75241 tttatgcggc attagctgag aaaaagctaa atttaaatgc atactttgtt attgttgcta
    75301 tacattcgct atacaataca tcagtttttg ttaatcatat ttttagttat cttggcagcc
    75361 tttgatccaa aaatattttg acaatgaaag cataatttaa agacaaccca ttgcaaagag
    75421 ttattatttc ttcaatgttt ctatagatca gcaagcatca tattcaaatt actttcttgc
    75481 tctttccttt tgaacaaccc ttttttgcaa gttaactctg ttcagtttgg cttcgtttga
    75541 gtttctgctc tccttgtttg ccaattgatt gtaggtccat tttggttcta ttccccttgc
    75601 tcggaagttt tcagcataat aatcttcatc gtatataaat ttccgtctca aacattagga
    75661 aattcaactc taattgaaat aatttacata taccctaaac ggaaaaccta ccgattgtaa
    75721 tgtatgtggg cagagtccgc cacttatgga ttaaaaaaat ccccgctgct cgcggtgctt
    75781 ttcacctttc cctcacggta ctggtccgct atcggtcttc cgggagtatt tagccttgcc
    75841 ggatggtccc ggctgtttcg gacagggttt cccgtgcccc gccctactca gggtcccgcc
    75901 cggtcgctcg ccgctttcgc gtacggggct ctcacccgct acggccggcc gttccaggcc
    75961 gttccgcctc gccgctcgca acctcctggc ggccctacta ccccgccgcg gccgaaaccg
    76021 ctgcggtttg ggctcttccc ctttcgctcg ccactactcg gggaatcact gttgttttct
    76081 cctcccctcc cctgctcgcg cggatttgca accgaccaaa gggagttcgg caaagccaat
    76141 ccgtgcggta acattaacat ttcacccgcc attgccggcg cggacttgta gtccgtgaca
    76201 aatacctctt acgctttact accctcgctc ccgcgcgttt ttaaacgcgt gcggcatacg
    76261 ccgctccgcc cggaagcctt cagtaaatgt agtaaaaatt cctttgcccg cgctcacaga
    76321 ttttttatac atccatcgca cgcgtatgga cacgcgcgcc aacatacggg gctgatggga
    76381 ttcggacggt aaggattacc tgggccggta ccaaaataaa aaagccccgc ggggtaatcc
    76441 cgcggggcgt aaggg
//
LOCUS       DAOSAL010000003        75423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_261_length_75423_cov_50.364452, whole genome shotgun sequence.
ACCESSION   DAOSAL010000003 DAOSAL010000000
VERSION     DAOSAL010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 75423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 75423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75423
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_261_length_75423_cov_50.364452"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(544..1224)
                     /locus_tag="PK049_00815"
     CDS             complement(544..1224)
                     /locus_tag="PK049_00815"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOK60128.1"
                     /translation="MKPDCKLCKMKSPAAELLRPNEIDTLEQNCAQVKFAKGETIFRE
                     GTFATNIVYLRSGLVKIHMKGPIGEKILRLNKAPAYLGLPTTFGDKVNHYSATTLVDS
                     EVCFISLDAFKKFIFSNGDFAYEIIMDLCRSELADFQRFTNLSQKQLPGRIAELLLCF
                     SNHLFKSNTFELPLNITELANFVSASRESVSRQFSELIKLGIIEITSGKKLIILRKEM
                     LEKISTNG"
     gene            1378..1923
                     /locus_tag="PK049_00820"
     CDS             1378..1923
                     /locus_tag="PK049_00820"
                     /inference="COORDINATES: protein motif:HMM:NF025066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HOK60129.1"
                     /translation="MEEEMLKQLEDLKKRVSKLEKNRKDQLSIAIVSGDLDKILATMI
                     ISLAAAASDTKVKLFFSFWALSALRDQKKKAKGKSFISKMFGLMLPKGRNKLKLSKMN
                     MMGMGPAMIKFLMRKQNVMSLDEMFKQAAELGIEIVVCEMSMNLMGFKKEEIIDYPHL
                     SYAGAATFVAEASQSSIQFFI"
     gene            1938..2303
                     /locus_tag="PK049_00825"
     CDS             1938..2303
                     /locus_tag="PK049_00825"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HOK60130.1"
                     /translation="MSQKIVIIVTHGPDNQEKATLPFVMATAAQAMDVEVTIILQSAG
                     VLLAKKGGCIENIHAPGLMPLKELLDTFLSLNGNLLLCTPCVKERAIKEDQLIDGSKL
                     IAAGTVITETLSADAVLTY"
     gene            2329..2574
                     /locus_tag="PK049_00830"
     CDS             2329..2574
                     /locus_tag="PK049_00830"
                     /inference="COORDINATES: protein motif:HMM:NF013379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HOK60131.1"
                     /translation="MEELKKLSVAQTVDARGTSCPGPLLAAKKAVGEIESGEILEVLS
                     SDEGTKNDIPKWCEKMEHEFLGIIEEDSYFRLFLKKA"
     gene            2634..3062
                     /locus_tag="PK049_00835"
     CDS             2634..3062
                     /locus_tag="PK049_00835"
                     /inference="COORDINATES: protein motif:HMM:NF014692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOK60132.1"
                     /translation="MKTEGYAPRILVFSTDKISDPGIDQAGLRKLHYSPSVYVISLPC
                     SSGVKPRWIMKAFEEGFDGVFIAADGHECSYSPKCAEHTNNIIVEAQRLMRERNINTK
                     RIRMAAICSVCAEPFVNHMGTFSKILAELGSVKNEIAGNS"
     gene            3101..5851
                     /locus_tag="PK049_00840"
     CDS             3101..5851
                     /locus_tag="PK049_00840"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOK60133.1"
                     /translation="MTTNNKYDALVIGGGIAGQEAALNLADMDYKVLLVEKELSIGGK
                     MIQLSKVFPTLDCAACITTPKMSETARHGNIKVMLNSTVKEIQKHNGAFTVKVNHGAR
                     YVKPEACTGCQQCEFACPEVRPDDYNTHLAGRKVAYIPFSLANPRIAAIDRNGTSAPC
                     ISACPGGVKPYGYVSLVRNGQYEEAMNLHLEDIPLPGSLGRACYAPCQGECTRGNLEG
                     AVDIRRIKRFFAEQYYNKFPEPPTREINANTNKRVAIIGSGPAGLTAAYHLALKGHKV
                     KIFEAAPKAGGMLMLTLPEYRLPKTVVERDIKNITSLGVEIECNKRVENITELKQQGF
                     DAVFVAVGTHQSSKMHIEGENLEGVEGCLDFLRNVNIGERYNLTGKRVIVVGGGNTAI
                     DASRTALRLGAEQVTVVYRRSREEMPCFAPEIKEAEEEGVKIDILCNPIRFIGQNGKV
                     SKVELVRMKLGEPDASGRRSPIPIEGSNYLVNADLVIEAVGLQPSTQPFANELELAKG
                     GTIKVNGKTFQTNIPYIFAGGDSVSGASTIIEAAGQGKKAAFYIDKYLNGLDIDTFEF
                     DEKLPAADKNEVIAKRHPALKKPVNDTYRKPAERVKDFNDVEHTLTEQQAKESAERCL
                     DCSNCRECHQCKTACPADAIDFGQKDELITVDAKTVIVSTGFKLFKPEHKPQYGYGIY
                     PNVIDAMQMDRLIAPTRPFNNVLRPGDGKIPDKIAYILCTGSRDSSLENAMACSGECS
                     NNPICSQICCMYSIKQAQLLMGALPMADITIYYMDIRAFGKGYDEFFQEAKSMGVNFV
                     KGRIAKIFPKDQPNGDLILRYEDVTTGKLKESKHDLVVLSVGVLPNSSISRVFSNATL
                     ELDEQKFIKQIDELVNPAQTSIPGVFVAGTASGPKDIPDSILSAGTAAAEAASYINTL
                     TH"
     gene            5880..7853
                     /locus_tag="PK049_00845"
     CDS             5880..7853
                     /locus_tag="PK049_00845"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HOK60134.1"
                     /translation="MKQRIGVYICHCGGNISDYVDVEQLSKLIANENNVVISKDVMFA
                     CSDSNQKEMIQDIQEKQLDAIVVASCSPKLHTHTFRNVALRAGINPYNYVQVNVREQC
                     SWAHSDTPLDATVKAYGLIRAGINRVAYSEALDNIEIKAQKAVAVIGAGVAGMRAAIE
                     LARLGNNVYLIEKEEQVGGRVAQNGKLFMTGEDGKALTQRLLDNVKSYSQINLFTKAT
                     LEKVSGSVGNFNIDVNVEGNLLKLNVGAILVTTGFDYYQPSENEYGFGQTNRVITLQE
                     FNKLVENKSKELSFDDKPVNSIAYIYCVGNRQSKGPNRYCSRYCCTAAIHSSITAHEK
                     FSGIKAFHFYRDIRTYGKQELLYRQSSLNGDVYLKFEEKEPPVIEVENGYPVVKVKDY
                     LTSRKEIALKADLVVLVTGMVARSDSNSVSDKLKIPVGNDKFFNEIHPKLRPVETVIN
                     GVYIGGACQGPKNITESVQSALAGVSKINALLKKEVIELEPIVARVNKETCLWCGKCA
                     EVCEYDAIKPLEVNGKMVAEVNVATCKGCGICAPVCPNDAIEIAEYSNHGIESMIAGF
                     AAQAEINQTETQHEEKDENALVGMKEYPKEWKTILESFNGTPLSIPQISNMSGLAKEL
                     VTYHLMTMNRYGIVEPAGLNDEESYYLYKPKNH"
     gene            7856..8923
                     /locus_tag="PK049_00850"
     CDS             7856..8923
                     /locus_tag="PK049_00850"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOK60135.1"
                     /translation="MPTINPNFAEEIKKFGAKDFNLCFNCGNCTAICSLSTNDETFPR
                     ELIRYSTLGLEDDLKSSLKPWLCYYCGECSTDCPRQANPGELMMSLRRWLTAQYDWTG
                     LSGLLYKNLWAYILAMIAIFGAVVGISYFSEFTHSELMHIGHLFEMVAIAGVFAIILL
                     PNIIRMWYFTIIKPGIKVNLKAYFLSLKDLLIHMFTQKRFLGCEPTRENKMRWLLHLI
                     LVIGYLSLLFTTVFLNWFSTTSNFIVWLGYIESAAIFLITILFMQKRMRKVDAMHKHS
                     QPSDWFFVIWLFLMGFTAFMVRLLIDTQQIDSFFWVYVIHITVLAQWALIIVPFGKWT
                     HFLYRSFAMYFDALIMAKEKK"
     gene            complement(8967..10004)
                     /locus_tag="PK049_00855"
     CDS             complement(8967..10004)
                     /locus_tag="PK049_00855"
                     /inference="COORDINATES: protein motif:HMM:NF016159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HOK60136.1"
                     /translation="MLDFSNAELTEVIVHSVGVDTDDIILSDTPLDIDDNLLNSLLRE
                     YFLGSFRNQGFYSFEHSSSLELNELYSYCSDFFEGNISFIEFSKNVANHLQHVSVHPN
                     IKGGELYIASFTDMVVDGELSDGIGIFKAESKERFLHVSRDAKGLKVTCQFGTTPRKL
                     DKACLVFNTEKDFGYKLCVIDNTNRDEAKYWLNDFLRAKLRNDDFYQTKQAINLCREF
                     VEEVVKPENNFVKVDQADILCKTRDFFKANEVFDLKEFEEVVLEEPKMAETFRDFKQK
                     YEEAIGCNIPDSFKISTDATRQSQNFFKSVVKLDKNFHIYIHGGRDRVERGFDPDTGL
                     NYYKLFFDKEV"
     gene            10224..10943
                     /locus_tag="PK049_00860"
     CDS             10224..10943
                     /locus_tag="PK049_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOK60137.1"
                     /translation="MGKLFDRLNSDVRFVEGINGCINCGTCTAICPAAEVYDYEPRTI
                     ANLVQMRDDNVIEELLKSDTIWYCGECMSCKTRCPRNNAPGLLIQALRGLSIETGLFV
                     ESEKGRQQLSLKRMLGNSILETGYCVHFDHAELSMFPELGPVWQWVRDNREKVLAKVG
                     ANYNGSGPGAMRKISKENLDELEKIFEVTGGLDWFDKIEDVSTQKAKELGMEKGDATD
                     KKSAYFTHVYRYNNAEKHRRK"
     gene            10965..12038
                     /locus_tag="PK049_00865"
     CDS             10965..12038
                     /locus_tag="PK049_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HOK60138.1"
                     /translation="MKQEGKKRIWSDYQKEIADDKFYFVRSCIRQTFFPGSDSTFIRI
                     LRDVLGKDIYENPDHTTCTGIGYHTEVVPFPTIQTVVARNFALMREKGYVNYTPSCVT
                     SFGIYTEILETWHHFPQEKEKTREYLRKATGREFEEPDNLAHTSDVLYKFRNEIAAKA
                     KYRLINKHTGKPLKVVDHIGCHYAKMFPSKGVGGAEYPQVLAGLVEAFGGEVIDYPER
                     RHCCGFGFRQYFVQANRGYSISASKKKFESMEPFQPDFIITNCPGCNYFMDRWQYALG
                     EMEGKTYGQNGEGIPVLTYEEVAGLVLGFDPWEMGMQMHQTDVEPLLKKMGVEYDPNQ
                     KYKGPNGEDLGVPMSPRFVNEKA"
     gene            12066..13088
                     /locus_tag="PK049_00870"
     CDS             12066..13088
                     /locus_tag="PK049_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOK60139.1"
                     /translation="MKKVAVIGGGVTGLEAANQLAQMGYSVTVFEKKNAPGGQVSKWD
                     RLFPNRRHSIDVVNELKSHLNGNPAIYLSTAINKITKKDERFQLEAYNRIFEADAVLI
                     ATGFEPFPAERKEEYGYGIYDNVITSAELEGMFKEHGHPIMRNGNAPKRIAFIHCVGS
                     RDEKVKRPYCSKVCCVTAVKQAIEVKEAIPEAEIFCLYMDLRMFGPGYEDLYKEAQQI
                     GINFIRGRLSEASELDGGRVLIKTEDTLSSKPLKMSVDMVVLMVGMSPAKGTDELIAA
                     LGLQTNKDGFVEITDYHLNPVKTKVSGVFTAGACTGPNNITDSVNAARSAAIEIHNYL
                     NSKQYD"
     gene            13081..13416
                     /locus_tag="PK049_00875"
     CDS             13081..13416
                     /locus_tag="PK049_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOK60140.1"
                     /translation="MIDFGFAPMPISIHNLDTANLSLAQWINQHDETFNHCIACGSCT
                     ATCTAGQFTTFSFRELCHAIRRGEVKNAIDESEKCMLCGKCTLVCPRNVNTRNIIMLI
                     RKANLTFRP"
     gene            13413..15251
                     /locus_tag="PK049_00880"
     CDS             13413..15251
                     /locus_tag="PK049_00880"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOK60141.1"
                     /translation="MRYHPFTLPFSIGLGFIVLYIVVMWTIWIIRLSRREQKIVWKRL
                     FSLRTVSAIWESIREGLLHRNIFKRNILLGYMHMSLAFGWFMLIVLGNLEARLYPHGV
                     INPPFYPIFFEFFRHDLTPLHEYEEIFTFLMDFFLLMVISGVSLAFFKRFRSRTLGMK
                     RTTRHILIDRIAITALWFIFPLRLFAESVTAGLYNTGHFFTNFLGQWVIYNLPLDYLY
                     YPAWWAYSIALGVFFITMPFSRYMHIPAEILLIFLRKYGVKEKDTHSTFTKIELSACS
                     RCGICIDACQLSTSLNISDTQPAYFIRDLRYSQPSERLRETCMMCMRCLEACPVGIEI
                     TRIRENERREYPLFTSTSYSYINGYKYPKAKVAFFAGCMGHLTPSVIKSTLALLDAAG
                     VDYTFIDKNGSICCGRPLQMNGQADAAKQLIAKNNHLIVESGAEVLVTSCPICAREFA
                     SSYHLTIPVLHHSQYLLQLTKQNRLRFNQSGLKVTYHDPCELSRGLNITREPRELLQN
                     AAILSNGSKEIESKSLCCGGSLGITNISMTDRYKIANNTLDEIVTESSSYLVTACPMC
                     RKTFTNAQEKIKVLDIAEILEKNLNFAPEKASDKVQTQAKHQAVTT"
     gene            15263..16444
                     /locus_tag="PK049_00885"
     CDS             15263..16444
                     /locus_tag="PK049_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004331988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOK60142.1"
                     /translation="MHNEKLGFNSLLIHGGMEPDPMGSATVPIYQTSTFAFENVDEGA
                     RCFAGESDGFIYTRIGNPTISALERQVAILENGFGGIATASGMGAVSSIYMAFLKAGD
                     HIVSTDAVYGPSRGIMETYFKRFGVESTYIDTSKLENIEAAIKPNTRMLYLESPTNPT
                     MVITDIKAASELAHKHGLVVVVDNTFCSPYLQRPLDLGADVVFHSMTKFLNGHADIVA
                     GIIVTKTQELYKTVRPAMVNMGCNMDPHQAYMVLRGIKTLSLRIERAQQNAQKVAEYL
                     EKHPKIEWVKFPGLKSHPQFELAQKQMKGPGAMISFELKGGIEAGKILMNNVRLAMLA
                     VSLGGVETLIQHPASMTHSKMSPEARIKANITDGLVRFSVGIEDVEDIIADLDQALAK
                     I"
     gene            16568..19282
                     /locus_tag="PK049_00890"
     CDS             16568..19282
                     /locus_tag="PK049_00890"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOK60143.1"
                     /translation="MKKYIALVSYVLMCVSVYAQVERMPFGDKMSYTANKLTIGNSKG
                     PSTPGSALFYEDFEGTTYQLTDKWEVLRSSTLDFQQTTAAANPSWFRCTPQSFNGNGS
                     TYIKHGEASGAISFTAPDFTWLITHDTIAIPSDPEAFLKFWLWYYSNYDQGYITHFYV
                     LAYDVDGQTLDTLLYYGNDQATSVPNQYKSLINLSLSKVAGKNVRLAFVYENRNNQNK
                     GTQLAIDDILVTNTNIPDIELKAIPYKYSKVPHITFDSLFIDLKANIYNLGTVLNDTL
                     TISVSCDKIPNLQSSAVITDTIESGEQRVITLSPKVKIMPTAESYTFNLNASTPPDTL
                     TQNNTDNTTLEVSSSIFATDRGAKGGISFNPNTQIGNLYEFQRDGFIEGVEIGWAQNT
                     TIPETEYPIDFVVYILEINPASPSEAKTLTQEYFTKEYSANGGSIIYFLPKPAYCHKG
                     FSYYVLVKQTSDTPLGIGYDGNPYGAFWKVDEFFPLNATYMANQSIGNIAVRALVSEP
                     VENPTVFFNIKNEQKEPAQNVKVRIVELDSTITTNEQGQASIELNNGKYTYSIDSTGY
                     SSLRKEFTVFSQNLIINDSLVKAYNVKFRIVDTDSVPLPGARINVYPITLTSNENGED
                     STMLPSGNYSLKVMLSNYMMKYPIDLLVEDKDTLYIVVMEQATTYNLTVNVKNTKGES
                     LPNADVSLMGYGTLKTNNEGNCAFNGLLPGNIMGSVKYYNYIWGSIFVDLTSDSTINV
                     TLIPEHYTATFYVTSKGNPIINAEIIIEGLDTLHTGLNGYAQSTYIPFGNNIPYTVKV
                     KDYYTYTGTFDIWDTDVLVTVNLTPLGVDDNYDDLNPTIYPNPTTDFITVTGINNYSI
                     SIFDINGRVVYQNSNPDSKIDIGFLPKGIYIVKLNTSKGFSIHKVLKQ"
     gene            19535..20851
                     /locus_tag="PK049_00895"
     CDS             19535..20851
                     /locus_tag="PK049_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOK60144.1"
                     /translation="MKNTPIPYEVVKRKIQESKLPSVGKASIRELVKLVNEIEKETGI
                     KYVRMEMGVPGLEPSQIGIDAEIEALKRGVASKYPMIDGVPELKKEIARFVKNFINID
                     VDEQGCIPTVGSMQGAMAAFLVVNRCNATKDTVLFIDPGFPVQKQQLRVLGMNYETFD
                     VYNFRGDKLRDKLESYLKKGNISAILYSNPNNPSWICFTEKELQIIGELATTYDTIVI
                     EDLAYFAMDFRKDLSKPGQPPFQPTVANYTQNWLMLISSSKAFSYAGQRIGTMVISNS
                     LYKRRYPDLKRYFTSDEFGYAMLYGAVYSLSSGVCHSTQFGFAAMLKAANDGTFNFVE
                     SVREYGEKAKIMKKIFTENGFTIVYDMDEDKPLADGFYFTISYPGLTGEQLIEELLYY
                     GVSAISLAITGSERTEGLRACVSQVQRNQFSDLEERLKRFNQDHRN"
     gene            20892..21128
                     /locus_tag="PK049_00900"
     CDS             20892..21128
                     /locus_tag="PK049_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOK60145.1"
                     /translation="MAKVRGAIVVDVEKCKGCGLCVVACPSKVIELAREVNGKGYNYA
                     YMANPEACTGCANCALVCPDTVITVYRVKTEAEA"
     gene            21168..22253
                     /locus_tag="PK049_00905"
     CDS             21168..22253
                     /locus_tag="PK049_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOK60146.1"
                     /translation="MAELKLMKGNEAIAEAAIRCGCDGYFGYPITPQSEVLETLMARK
                     PWEETGMVVLQAESEVAAINMVYGGAGCGKKVMTSSSSPGISLKQEGITYIAGAELPC
                     LIVNVVRGGPGLGTIQPAQSDYFQAVKGGGHGDYKLIVLAPASVQEMYDFVDLGFELA
                     FKYRNPAMILSDGIIGQMMEKVQIGPYKPRMTDEEIVAKYGSWATTGKTPNRQRNIIT
                     SLDLDAGRQEQFNLKLQRKYKEIEEKEVRFEKIACDDAEYLLVAYGSSSRICQKVVDM
                     AREKGIKVGLLRPITLFPYPTKAIQAMLGQLKGILAVEMSAGQMVEDVRLAVEGKVKV
                     EHYGRMGGMIPTPNDVLKALEEKIIKG"
     gene            22259..23020
                     /locus_tag="PK049_00910"
     CDS             22259..23020
                     /locus_tag="PK049_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOK60147.1"
                     /translation="MDVKDIIKEENLVYSKTKLLTDNIMHYCPGCTHGVVHKVLAEVI
                     DELGIQDKTIGVAPVGCSVLAYNYIDIDWHEAAHGRAPALATAINRLYPNKYVFTYQG
                     DGDLASIGTAEIIHACNRGENIVVIFINNGIYGMTGGQMAPTSLLDMPTSTTPYGRKA
                     ELNGYPLKITELIAQLPGTCYVTRQSGHTPAAVRKLKKAITKAFENTGKKKGTSFIEV
                     VSTCNSGWKLSPVKANEWMVEHMFPYYPLGDLKDE"
     gene            23048..23593
                     /locus_tag="PK049_00915"
     CDS             23048..23593
                     /locus_tag="PK049_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOK60148.1"
                     /translation="MTEEIIIAGFGGQGVLSMGKILAYSGIMQDQEVAWMPSYGPEMR
                     GGTANVTVILSDSRISSPILQEFDTAIILNQQSLDKFEKMVKPGGTLIYDPNGITRHP
                     QRTDINIYRVEAAEEAARMKNAKTFNMIVLGAFLKVKPIVKLENVIKGLEKSLPQRHH
                     HLIPMNQEAISRGMEIVKKIN"
     gene            complement(23641..24801)
                     /locus_tag="PK049_00920"
     CDS             complement(23641..24801)
                     /locus_tag="PK049_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005923341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOK60149.1"
                     /translation="MKNFEFFNPVRILFGEGQIDMITQELLPYQTILLTYGGGSIKRN
                     GVYDKVISALKGKKVLEFSGIEPNPRYETLIKAVKLARENNVDFVLAVGGGSVIDGTK
                     FIVASIPFEGEPWDILTKGIKVEKAVPFGTVLTLPATGSEMNAGAVISREATKEKYAF
                     GSPAVYPKFSVLDPTTTYSLPQKQVANGVVDAFAHVLEQYLTYPSNSPIQDRFAEGIL
                     QTLIEVGPKTLENPTDYDLRANFMWSCTMALNGLISTGVPTDWATHMIGHELTAFFGL
                     DHGVTLAIIYPALLTEMVEFKKEKLLQYGSRVWNLNGKNLTPERAIEETEKFFRGLGV
                     KTRLPEHGISFADTKPIVDRFAARGWKMGENKNITSDIVEKILKRAAELYRQ"
     gene            complement(24880..25185)
                     /locus_tag="PK049_00925"
     CDS             complement(24880..25185)
                     /locus_tag="PK049_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60150.1"
                     /translation="MKKVQVSKNKVKNYLSERLARSIVDADENALITVLRYNAIGGFE
                     YLSDEDLFEFLSTSIPELDFVRLAGADDDNLQLEVKKEYKDEEDAIIIDIQRAIQVI"
     gene            25361..26032
                     /gene="ung"
                     /locus_tag="PK049_00930"
     CDS             25361..26032
                     /gene="ung"
                     /locus_tag="PK049_00930"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOK60151.1"
                     /translation="MKPQIEETWKNVLMDEFKKDYFIKLKEFLVEEKKKYTVYPPGSQ
                     IFAAFNHTPFNSVKVVILGQDPYHGPGQAHGLCFSVPKGTPAPPSLQNIFKEINSDLG
                     LPIPNHGNLEKWAKQGVLLLNATLTVRANQAGSHQNKGWETFTDAAIKALSDQHKGLV
                     FILWGNYAQAKTCIIDTNKHFILTAPHPSPLSASRGFFGCKHFSKTNRLLTSISKEPI
                     DWSLD"
     gene            complement(26033..26758)
                     /locus_tag="PK049_00935"
     CDS             complement(26033..26758)
                     /locus_tag="PK049_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60152.1"
                     /translation="MTAVAKIIETRGVMVKEEKVKSLSERIYSNTMVLENYEPYPGYY
                     GKNVPTDVIPRSIFLILVREYDHMFLARTMQKLSREAKHACMGTYGYIDLEDHRYYCI
                     RIKNLRCFEEIPIIQQKLAEHGIDFRRSKPVNSMALVTIEKTFLVERFDEGIYLDLAG
                     DNKYYIEIPQELKWDDFKLITSHVKNNLQNSFFDAALGYFWKVDGPQDVIRIYDLKAN
                     LERISEIRSKYLQEIERFNKTHS"
     gene            complement(27015..28913)
                     /locus_tag="PK049_00940"
     CDS             complement(27015..28913)
                     /locus_tag="PK049_00940"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HOK60153.1"
                     /translation="MNLNNLPIGKRLALGFSLIIGLILVVAIAVFISNRKVRLESNKQ
                     VMLNNLLASNAALSSHLLDFIQSGNQVELDSAKFYFTKFKNQLEILKPVVTKKNYGLI
                     TIIEGNIKVVEDQFAILENSNQFIAQTATVLESSSNTALTIAARVMGGEVSRGVQQFL
                     NIRIMEKNYLISGNDELYRKWLTEIDDNTRLASRLGLSEIVRALEQYKGSFIKHVEAK
                     KVIFDAKSKSLEAVTHTQSLLQKGVILSAGLLNDSAKHGLTVIIFVVLSIVILSSLIA
                     YRVTRSITEPVNSGVELAHNLSKGNLVIHASESAKRGDELGKLSRALNDMSEKLKEVI
                     STIYISSNHIAAASEQLSTTSQQLSQGASEQASSAEEVSASMEQMVANIQNNTENAKQ
                     AELIAFKSVEGIRKGYDSTTYALNSMKKIAEKVTIIGDIAFQTNILALNAAVEAARAG
                     EHGKGFAVVAAEVRKLAERSRLAAEEINRITHDGVKIADDAGRLLSEIVPDIEKTARL
                     VQEIAAASMEQNAGADQINSAIQQLNQVTQQNAAASEEMASGAEELNSQAQQLIDIVS
                     FFDTGGKETLTTLSNLKNVTKKIAQTSITPKTEFRESKRTKSKPKLSDGIDIKLSNNS
                     DDTNYEKF"
     gene            complement(28926..29165)
                     /locus_tag="PK049_00945"
     CDS             complement(28926..29165)
                     /locus_tag="PK049_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60154.1"
                     /translation="METISNCPKLEKCPIYLKNVFFNPNAGETYRKIYCTAGKEKYTS
                     CKRYLVSEKVGKPVPESVMPNCSLTVDEIIAKYNL"
     gene            complement(29764..29837)
                     /locus_tag="PK049_00950"
     tRNA            complement(29764..29837)
                     /locus_tag="PK049_00950"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29801..29803),aa:Asp,seq:gtc)
     gene            complement(29912..30454)
                     /locus_tag="PK049_00955"
     CDS             complement(29912..30454)
                     /locus_tag="PK049_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HOK60155.1"
                     /translation="MRIVSGSLRGRLINPPKNFRARPTTDFAKESLFNIIANHFDFEG
                     LRVLDLFSGTGSISYEFISRGAALVDSVEKDFHHWQFIRNTANQLKLIGLNPIKGDSF
                     SYIKSCNRQYDIIFADPPYDLQGIDTLPQIIFNHPVIVDGGWFILEHSNKYNFTDNQH
                     FLEHRVYGSVNFSIFQKKNF"
     gene            complement(30445..31293)
                     /locus_tag="PK049_00960"
     CDS             complement(30445..31293)
                     /locus_tag="PK049_00960"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HOK60156.1"
                     /translation="MQEIDIIDETFDRDRTSTYELSIQVSLNGFSYAVKDTIRNTFIA
                     LFNTGWQGFSIGSVDFRNFIGKLTEAKPWLKGEFKKVIVAFNLPYFTIVPNEFFEPSK
                     SKLLLEQVHSIPDGFELGFTELPSLSLVIIFAAPHEFINEWKSLHGGSLFTHSLLSFI
                     QVPYSYTAKPFLQVDIDQQHLAIILHNNGQLLAANSYLCKSTTDVVYYTNALARSVID
                     NTDNLQLNLTGDGELMAGLPEEISKYFNSITHQANFHKPVFFSYRLLRYRAEYFRLFN
                     LGVTCE"
     gene            complement(31304..31903)
                     /locus_tag="PK049_00965"
     CDS             complement(31304..31903)
                     /locus_tag="PK049_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60157.1"
                     /translation="MKTVYQIALAVIIAILGYFLYESIMEPIRFEKEKNRRYNATIER
                     LKSIRTAQDAYRSRFGKYTGSFDTLINFMKEGQIKVVKQIGSEDDSLAVIQKKVFRDT
                     ILIPVRDTILKGYPYDSLRFVPFTGGEQFELAAGELKTQSGVSVKVFECKAPNKVILK
                     NLDRQEIINMDDLAKKLNKYPGLKVGSLTEATNNAGNWE"
     gene            31991..33400
                     /locus_tag="PK049_00970"
     CDS             31991..33400
                     /locus_tag="PK049_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOK60158.1"
                     /translation="MIKTHVIKEIVERLGFAPTVGQLEAINTLSEFTVQPTGNDCMLL
                     TGYAGTGKTSLIAAYVKALDKFGINYQLLAPTGRAAKVLAEYSGCNAYTIHKIIYRQQ
                     SNTDGFGRFTLNFNKSKNTIFIVDEASMISNQSFDDSAFGSGKLLTDLIEYVRMGKEC
                     KLMLVGDTAQLPPVGLDISPALNPAELESFGLNVRFAQLSEVIRQQFDSGILQNATWL
                     RELVDSSKNVFPKFNTEYADVKRISGGELLEYLSDEYSKNGREGNIVICYSNKRANRY
                     NQGIRAKILFHEEELSVGDYLMVARNNYYWVNDRPNIGFIANGEIVRVKRIGKRTELY
                     GFRFADVTFELLDYNNEEFDAKLILDSLYMDGPSLGSESMKMLYQEVLADYMHLKTKK
                     ARMKQIQNDPYFNAIQAKFAYAVTCHKAQGGQWPTVFVDQGFFNDDMLTREYLRWLYT
                     AFTRATQKVYLVNFDDKFF"
     gene            33500..34222
                     /locus_tag="PK049_00975"
     CDS             33500..34222
                     /locus_tag="PK049_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60159.1"
                     /translation="MKRVFYVLIAVLATFNAEVKSQESQSIKTQLKFDFVSRHLWRGM
                     RNNTTPAVQPTIRFDGNMFFGGFWASYSLGSENVQEIDIYTGLKYKNVDFTIIDYYNP
                     IDTAGWKGDFFEFRNSKTRHTLDAIITINPTSHFPLSLTFATMFYGYDKDSLSKKNLY
                     STYLEAEYTFFKNDNTRISFHIGGTPYKSYYASKTAITNTGITVTRNIHFNSNITIPV
                     KGSFILNPYTHQVFLLAAFTIL"
     gene            34474..36573
                     /locus_tag="PK049_00980"
     CDS             34474..36573
                     /locus_tag="PK049_00980"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOK60160.1"
                     /translation="MRYRSLRTEMMVNILGITIVIMAVTLTVVAFKSINTAKKTARET
                     SSLIASDVTRSVASYLEKPFETLYNINVTFSSLKLSGVKNREVYKDVLKKTLDLNKNY
                     LALWAMYEPNMLDENDSKYAGKDGYDEEGRFDISFYKANNTIKSEAGEVSMYSEDYYQ
                     IPFTSGNETVLEPYFYTYTGDETGHSYFETSVAIPVKESGKTIGVIGLDIDLLELSEN
                     IKNQKVYQTGYIFIASYDGIIAAHPNKELTGDTLSNILKIEIADYKKIVDSNSFSTVT
                     LGKGSKQSFVFINPIKIGKTLKPWSVVVVVPVNEVYSESRKLIITIVIVGIIAVALLI
                     MIILYQAGNITMPIFKAVRFANNIANGDLTHKLEHNRSDELGTLQSSLLQMQQKLIEM
                     VIEFKSSSGSIADASSHLNSTAQQVSQSAEELASSSEELSSTMEEMVSNIEQNSHHAI
                     EVEKISLELVTDAQKVKDASERSMASIGSIAEKIQIINDIAFQTNLLALNAAVEAARA
                     GEHGKGFAVVAAEVRRLAERSRMAADEINIQSGESVQITAKATELLNQIIPKIQKTTE
                     LIQEIAHASKEQLTGSEQVNNTTQQLSGVTQQNATISEELAASAEELAAQAEKLLEFA
                     ESFKIETAHGVKKDDSKHQTIESEKTTPKLEPKPKTSEKKVIKPLTKKKAEEPKQPHK
                     GIDIKLKPSDDSEYESF"
     gene            complement(36609..37295)
                     /locus_tag="PK049_00985"
     CDS             complement(36609..37295)
                     /locus_tag="PK049_00985"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HOK60161.1"
                     /translation="MTNRIMDPIGRLMGLRYKSHPWHGVDVGPNAPSIVTTFIEMVTT
                     DTVKYEVDKVSGYLRIDRPQKYSNVLPALYGFIPQTLCDENVAQIARDKTERNDLAGD
                     GDPLDICVLTEKNIAHGNLLAYAIPIGGLRMIDGNQADDKIIAVLKDDAVYGHIRNID
                     ECPEAIITRLRHYFLTYKDLPGTHRDCEITHVYGVDEAHQIINAALDDYRKKFENLDM
                     ILSQYGMMKP"
     gene            complement(37340..38353)
                     /gene="murB"
                     /locus_tag="PK049_00990"
     CDS             complement(37340..38353)
                     /gene="murB"
                     /locus_tag="PK049_00990"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOK60162.1"
                     /translation="MFVIKKDISLKPYNTFGIDVKASYYARANTVEKILYAINFASYN
                     KLPIFVLGGGSNILFTRNIEGVVINPAIQGVQLQTDDEQYYVFRVGAGVVWDKFVEFA
                     AENNLGGIENLSHIPGLIGASPIQNIGAYGVEVKDTIVKVEAIEVSSRKLIEFNASEC
                     RFGYRDSIFKQELKGKVIITHVWFRLSRNPSFILNYGNLAEEVEKLGEVTVQNVRKAV
                     INIRKSKLPDPAELGNAGSFFKNPVVDADKYNALKSEHPDLNGFQVSESFYKIPAGWL
                     IEKAGWKGKQVGRCGVHHKQALVLVNHGGATGTEILELSSSIQKSVKELFGIELEREV
                     NVF"
     gene            38471..39562
                     /locus_tag="PK049_00995"
     CDS             38471..39562
                     /locus_tag="PK049_00995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOK60163.1"
                     /translation="MRIQIFSTSYIVTDQRLQRVANSLAAYGYSVKVFCRKHKNLSYE
                     NNKFNVKYINPLFEKGPLFYFFYNLRIFLRILFSRSDIIYSNDLDTLPGCALGSIVRC
                     KKLIYDSHELFTEVPELIGRPITKFLWHIQEKIFVKRAKAVITVSEGVANELKKRYNL
                     KNIEVIRNVPIRTSTEVFKDKQPTIIYQGALNMGRGIELAIEMMKQLPCYKLLIVGKG
                     DIEIKLRRQMLESDLSGRVQFLGQLTPENLRLVTPTAWLGLSLEEDMGLNYRYALPNK
                     LFDYLAAHVPVLVSDLPEMKRVVEEYGIGIVAQSRKPIDLANQIADFFEDKEGYENTL
                     KNVKKAAEKLCWQNEEPRLLNLINSLKKG"
     gene            complement(39559..40119)
                     /locus_tag="PK049_01000"
     CDS             complement(39559..40119)
                     /locus_tag="PK049_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOK60164.1"
                     /translation="MFTNHSDYFYNLVQNRQSDRAYDTRPVGREVILRIIEAARLAPS
                     ACNAQPWHFIVVDEPELKNKLADAAASRVLGINHFTKQAPVHIVIVMEPANLNSNFGS
                     LVKGKTFPLIDIGIAAEHICLAAKAEGLGSCMLGWFDEPAVKKLLDIPKSRRVPLIIT
                     LGYPASDEVRQKRRKDFNEIVSFNKY"
     gene            complement(40103..40762)
                     /locus_tag="PK049_01005"
     CDS             complement(40103..40762)
                     /locus_tag="PK049_01005"
                     /inference="COORDINATES: protein motif:HMM:NF024852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HOK60165.1"
                     /translation="MERKAAVFGSTGLVGYNLLRVLDENRNYHEIVAYRRNLNQSNPF
                     QKVRFVEFGANISIEPDTYDVFICLGTTMKKAGSKEAFKAVDLDMVIEIVHKASQAGV
                     KRIVAISSIGANQSSRNFYLRTKGQMEEELKKFDFELVAIVRPSILLGKRNEYRLGES
                     IGIWLFRAFRFIFVGPLRRYRGIDAGDVAKAMVQLALTAKGKVTVESEVLKQIADVYK
                     P"
     gene            complement(40794..43184)
                     /locus_tag="PK049_01010"
     CDS             complement(40794..43184)
                     /locus_tag="PK049_01010"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOK60166.1"
                     /translation="MKKLFLITFVLIGFEAFSTNILTQTVRGVVIDKNTKMTLPGANV
                     ILLDSDPLRGVMTDEQGRFKFESVEIGRVSLKVSFLGYNDVVLSNLNLQVGKELVLNI
                     EMEELAVNVEAVVVTAKSDKSTPMNKLATVSARTFTVEETERYAGSRNDVARMAANYA
                     GVMGVDDSRNDIIIRGNSPMGLLWRLEGVDIPNPNHWGATGTTGGPVCMLNSNLLENS
                     DFISGAFSAEYGNAISGAFDLKMRSGNNEKYEFLGQIGFNGFEFGAEGPIAKSNGSSF
                     IVNYRYSTLGVFDKLGMDLGTVGVPYYQDISFKINLPRTKLGNISLFGLGGLSDIEIW
                     DSRRDTLKDKIDFYGGEGFDLTNGSSMGVVGLNHSFMVNQSTYTKLSVAVSAHQFKTV
                     MDSLSTDLLQKFPYYRNNFIESTISGNLSFNKRFSTKSQIKIGVIAKLLAFNYYDSVF
                     KKNDNAFLILRNDEGDALLTQPYINWQYRPTNTLTFNTGLHAIYFFLNREWSVEPRIA
                     IKWAISSNSSINLGYGLHSQVVSLSIVLGRVRLSDDSYIQPNKDLGFVRSHHFVAGYD
                     WLLASHTRLKAEVYYQYIFDAAVDAAESNSYSLLNQGASFVLELPDYVKNGGNGQNYG
                     VELTLERFLNKGYYYLLTASLFNSQYQGSDGITHSTAFNHHYITNALFGKEFNLSNSK
                     KSKKTFSFDAKVSYSGGKRATPWSAVYNPTENRYSREWDTSKAYELQLRDYFKTDLTI
                     RYKLNKLGFTQEWAIEVTNLFNNKNIYGDRFNTRTGESQWVYQLGIMVIPQYRITF"
     gene            complement(43332..45026)
                     /gene="ggt"
                     /locus_tag="PK049_01015"
     CDS             complement(43332..45026)
                     /gene="ggt"
                     /locus_tag="PK049_01015"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HOK60167.1"
                     /translation="MGKKILSSILLLTGITLTLFSQDRMTGLNFATRSEVIARNGMAC
                     TSQPLATQVAIDILKQGGTAVDAAIAANAMLGLVEPTGSGIGGDLFAIVWDAKTKKLY
                     GLNASGRSPMSLTLEYFKKNGIAHIPALGPLPVSVPGCVDGWFELHKRFGRLPMQKIL
                     SPAISYAREGFPVSELIAYYWQRNAVRLKQYEGFADVFMPNGKAPSKGEIFRNPYLAN
                     TYELIALKGRDEFYKGSIAKAIDEYMKRNGGFLSYEDLATHKSEWVEPVSSSYRGYDV
                     WELPPNGQGIATLQILNILEQFDIKSMGFGSAEYMHLFIEAKKLAFEDRARYYADPDF
                     VKIPYEILISKEYARERAKLIDLNRAARTYDAGMLQTSNTVYLTVADREGNMVSLIQS
                     NYRGMGSGMTPTGLGFVLQDRGELFSLEEGHPNVYAPGKRPFHTIIPGFITKDGKPWV
                     SFGVMGGGMQPQGHAQIIVNLIDFGMNLQEAGDAPRIQHEGSSEPTGERMTDGGMVYL
                     ESGFDYQEIRKLLAKGHRIGFSLDGYGGYQAIMFDEKNGVYIGASESRKDGQAAGY"
     gene            complement(45080..47992)
                     /locus_tag="PK049_01020"
     CDS             complement(45080..47992)
                     /locus_tag="PK049_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOK60168.1"
                     /translation="MKQILKKIQLLLFAVTVFTANALAQQYKYETVPNDPMGVRIYTL
                     DNGLKVLMSVTKDEPRIQTFVAVRVGSKNDPKETTGLAHYFEHMMFKGTPDFGTLNWE
                     KEKEMINTIEQLFEVYRNETDSLKRAAIYHKIDSISFEASKLAIPNEYDKLMSAIGSR
                     GTNAATSNDYTIYIENIPSNQLENWAMIQANRFSHPVLRLFHTELETVYEEKNMSLTN
                     DARKVGEALLRGLFPNHPYGQQTTLGEAEHLKNPSMKNIREFFDKYYVPNNMAVILAG
                     DFNPDEAIKVVDKYFGTLKAKPLPEFSIVPEEPIIQPKEYEVVGLEAENVSIGFRFAG
                     ATSADADMLSLINSILYNTKAGLIDLNINKKQLALRAYASARVMTDYSILQLGGRNKK
                     GQSLEEVKDLLLKQVELLKKGEFPDWMLEAAINNYKLNRMNALESASSRARLMMNAFL
                     NGIEWENVVNSIERLQKISKDDVVKFANDYLGNNYVVVYKRQGKPQDVAKVAKPPITP
                     IHINRDVESDFLKKVKETKVTPVEPVFIDYNKDFTKIKINKNIEVLYKQNTENGTFNL
                     VYYYPFGSLSNPALTHAADYIQLLGTSKLSAEEVSQEFYRLACTFNINVNDEDTYITL
                     SGLSENAAKALNLMESLLNDCQPDESALKAYIANQIKARNDAKRNQSAVFSALVSYAT
                     YGDNNPQKFILSEKELNELKANDLIGVIKSLSSYRHTVLYYGPLASKDLKVMLGKEHR
                     LPKKFIPTPENKRFEPLETTSDRVLFAHYDAKQSYLQTLSRGQKYDDNLYPIITLYNE
                     YFGGGMNAIVFQEMREKRGLAYTARSRYTIPSRPDDYFINMSYIATQNDKVVDAFNAF
                     NELFNAMPENTIGFNLSKEALISDIRNNRIRNMRVLYSYLNAKQMGWNEDIRKKLYSQ
                     IPTYTFDDVKKFQEQFVKGKPKTYIILGKEADMNFQELEKLYGPVTKVTLEQIFGY"
     gene            48122..50425
                     /locus_tag="PK049_01025"
     CDS             48122..50425
                     /locus_tag="PK049_01025"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOK60169.1"
                     /translation="MKRKLKIRQKILLYILTVTAILYVASVGYILWQSRANIYSDAIE
                     KTQLTAKVSAKEVGAIFEKELSLVRTLSQAFTVYKNMPHEQWKKLFLDMYIPVLRENP
                     EIYSIWDSWEYKSFVPGYDKDYGRFVITTWREGNEIKWLYDERSINGDPPIYGKYKKL
                     GQEAIWEPYVDQVTKGKADIKLMITYYSPIYINNVFSGIVASDVSLESLQEMVSKIKP
                     VKGSFAYLISNEGIIAAHPSKDFINKKIDEAFKIDNEKEKIAERIAKGEEFYYTKTDE
                     TGKQLLICHSPIIAGKTKTPWSLALSVPVDVIMANAKETLYVSLFVSIAGLLVMVLVL
                     LFVSDNLTKPIKRITDILKRMARGEVSDELKVDLNTGDEIEEMALALNTTIDGIGHKT
                     TFANEIGKGNYTTYLELLSDKDVLGKSLIHMRDSLKKAAEDEEKRKVDDQKRSWANEG
                     YAKFGEILRQNNNDLQKLCDSVIAALVKYVGANQGGIFLWNDEDKTNQYFELVSAFAW
                     DRKKYLTKQIEKGEGLVGACAMEKETIFMTDVPDDYVNITSGLGDANPRCVILVPLKH
                     EDEVLGVIEMASFKVLEKHEIEFIEKIAESIASTILSVKINARTKMLLEQSQMQAEEM
                     KAQEEEMRQNMEELQATQEEVERKSAEIEEFIKSIQSSAYVIEYDLNGNILNVNDSFI
                     QFVGVPREQLIGSHHSDNLELSDVQKKEYGTFWDNLKAGISKKVKSTITWKGKTYHFL
                     ETYIPVKDSEGRVYKIMKLAFEDGEFK"
     gene            complement(50457..>51131)
                     /locus_tag="PK049_01030"
     CDS             complement(50457..>51131)
                     /locus_tag="PK049_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HOK60170.1"
                     /translation="LVTAKNTTAMLTTFNEVDMSRVQEIRARYQTDFQQKFGVKLGLM
                     SFFVRAAVEALRLHPMTNSMIDGEDIVTPSYIDMGIAVQTPKGLMVPVLRNAQTMSFA
                     EIEKEILRLATKAREGRITLDELNGGTFTITNGGIFGSMLSTPILNYPQSAILGMHNI
                     VDRPVAVNGKVEIRPIMYVALSYDHRIIDGKDSAAFLMDIKRMIENPEKLLFGGDDPI
                     MKLIGL"
     assembly_gap    51132..51231
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(51755..51880)
                     /locus_tag="PK049_01035"
                     /pseudo
     CDS             complement(51755..51880)
                     /locus_tag="PK049_01035"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(51903..54701)
                     /locus_tag="PK049_01040"
     CDS             complement(51903..54701)
                     /locus_tag="PK049_01040"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HOK60171.1"
                     /translation="MENILGNIDPKTIDELYRMWKSNPNSVDEGWQKFFLGFDLAISG
                     SYSAQDCIADSEFKVIKLIEAYRMRGHLFTKTNPVRTRRSYSPTLDIENFGLGTKDLD
                     NRFKAGELIGLPNATLSEIIERLNRIYCRSIGVEYMYLREPKLVDWIQSRVEGTQNHT
                     QFSPDQQKHILYHLTAAVGFEQFIHRKFVGQKRFSLEGLEALIPALDTTIEHGAEHGV
                     KEFVIGMAHRGRLNVITNIMRKPYEEIFAEFIGEAYDDESTLGDVKYHLGYSNTIETD
                     KGKMVRLHLVPNPSHLETVGPVAEGIARARIDDAYQGDVNSLIPIIIHGDAAVAAQGV
                     VYETIQMSRLSGYSTGGTIHIVLNNQVGFTTNYIDARSSTYSTDVAKVTLSPVFHVNA
                     DDPEALLHVIRLAVEFRQTFHRDVFIDLLGYRKYGHNEGDEPRFTQPVLYDLIAKHPN
                     VRDIYTSKLVETKVISVEDAATIRKNYDTMLEERFVLAQANPKIRIGRFIPEKSSSYR
                     YSSSNDFEKSPDTGVSKEMLNMVSNALLELPSGIDFFKKLVKIIDERRENYTQQKVDW
                     AMAELLAYGTLIAEGHPVRLSGQDSERGTFSHRHSVYSIQGSEEKYYPLQNIPNTRAK
                     FSVYNSLLSEYGVLGFEYGYSVALPNGLTIWEAQFGDFHNVAQVIIDQYISSAEDKWG
                     LQSGLVLLLPHGFEGQGPEHSSARIERFLTLSARNNMQIVNATTPANFFHVLRRQLKR
                     DFRTPLVVFTPKSILRHPKNISTLQELESGSFLEVIDDSNVDEKAVSRVVFCSGKIYY
                     DLLQRKEELDVKDIALVRIEQLYPFPSRQVEKILDRYPNAKKWLWVQEEPQNMGAWNF
                     VKDRITGVPLEVISRAPSGSPAVGLSKIHNLEQKEIITKVFRPCTCELKNKYCGLQCE
                     EGSRRFDRLNQYEYFERK"
     gene            complement(54787..56193)
                     /locus_tag="PK049_01045"
     CDS             complement(54787..56193)
                     /locus_tag="PK049_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60172.1"
                     /translation="MERDILTTKRKALAINLDPTIYGTIAEIGGGQEVARAFFQAGGA
                     SGTIAKTISAYDKVFSDYLYSDGDSGRYVCLKRLQQMLDAEYKQLIDVLNSERSPDKR
                     FFVFANTVETLNFRKDNQGHGWLGVRFELTKLGTANEVVIHVRLNENDAILQQYTLGT
                     LGVNLIYACFYNHKSPTLFLQSLMDNLSTDRVEITMVRMGGPDLDYVDNRLLGVQLVK
                     NGMTPAIFFDRYGNVQEPDDMLYKKNALVLRGRFRPITYVGFDILKTSYAHFKKNALF
                     KPDNTLQICEITLNNLLEEGELKERDFLDRVDLLNGMGQNVLISNFREFYRLVDYFGR
                     FKHKNLRIVMGIPTFMKVLDKSYYANLPGGLLEAVGKMFPARTKFYIYPALDRNTGNI
                     RYITDITVPDDVMHIFHYLMQNDLIVSLTGVNEQWLKITSDEVLSLIQSNDKRWESMV
                     PKYVVKTIKQKKLFGYTG"
     gene            complement(56426..59089)
                     /locus_tag="PK049_01050"
     CDS             complement(56426..59089)
                     /locus_tag="PK049_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOK60173.1"
                     /translation="MKKLLFLFAVFLFSKVSAQTIIEGYVSDFKGKPIDHAQIVLYST
                     SGSGILSYAFVNTSGYYKITTSKQGDFRIEYLALGFNKIVKFIKIPLDSALVNPIRID
                     VTLEEQAYEISEVIKHGDKPITIKTDTIVYNVKSFTNGTERVVEDVLKKLPGINVDDN
                     GRVTFKGKEVEKVMVEQTDFFGKGYRMITQRVHANAIEKVEAISNYSENPILKEISNS
                     DKVALNLKLTENSYKKIYTNFDLGYNTINKHDINVSLMNFNRVVNSLIIGNSNSIGDN
                     PTVGSSELFKTLSETSDDKLTKADSPDFILTQDAYRPILKRQRVNDIDLILGSMNEAV
                     NLSKKTKLTVTTVYSNINDKFIRQTLKNYSYDTLSIINTENYNFYMNNKNAFIKSKIE
                     SSPSNKSFIQYNFLFSSTYKDANTLTDFNQTAVGEEGTGSSFKLINHLNFSQKINNRT
                     IAYLIGYTLNGNLKEELMINPIPITHLSTIGSIDGRGKQKLDNDYCYFLGVTGIKHRI
                     SNMLLIEGEVGANIISYDLKSSYVIIDSLNNNTPDPSFLGNTNINSNEAFLQSKLIWG
                     SDSLNISLGYKICLNKIYEKQNNYHYKRIILLPNITFKWLLNRINTLSLFYNSDIKPF
                     PYTSIINHYYFNSYNSINRGTFPSLMNMHNLGASYLIGKLNTRFFMNSSLMYIYESKF
                     ITQNIEPNINYVVSKDTITKNRNTIIGNIEANYFIKLIRSNIKFSLTSLYFDYDNFLF
                     GISNRNRNNSTTLAFEIRSSFNSFLNFHSGYSITQGRIVNKKTKQNNIERIFCNLYIK
                     PSQNLSLDLLSELYSNQSTVLTYSNNSYFLINSKLTYNFPNTKWSMSFSTFNILNDNN
                     YVVSSISEYYYSKVSYNLIPRYFMFSVSFKY"
     gene            complement(59165..60043)
                     /locus_tag="PK049_01055"
     CDS             complement(59165..60043)
                     /locus_tag="PK049_01055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLPGLI family protein"
                     /protein_id="HOK60174.1"
                     /translation="MRNRVFAFVGSVLFISNIAISQTERLSGSVVYESVWTTTYPIQA
                     KGVLCFNQYGSSFMAISDFTAVESNGKGNDSRNENLSMVETAPGQFSVVRRRDPNTLK
                     ESKSIPFNIFTVKENSKIYQLVNETYFANKKKQTVPYFYLEEEVGRIEWIIESETKTI
                     GSFQCQKATCKFRGRDYIAWFTPEIPITYGPWKLHGLPGLIVQVADIKNEVIFSAVEI
                     NIGNVTSCNIAYKKEYPVYTLREYITSQRERVQNESKRLESNIQVIQARQPEDIGFNV
                     SSFKVHNVGLELEYEF"
     gene            complement(60134..60469)
                     /locus_tag="PK049_01060"
     CDS             complement(60134..60469)
                     /locus_tag="PK049_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60175.1"
                     /translation="MKRLILLFCLTLTYGTLFAENKIGAMEPSAKAVVTVSINLNVDL
                     SDQMDNSQNSNPEDKTCYATFTFSTDCGNGTVYHYSVFVWGKCTSDFYRRAAMAALDV
                     AEAYDQILC"
     gene            complement(61192..63246)
                     /gene="htpG"
                     /locus_tag="PK049_01065"
     CDS             complement(61192..63246)
                     /gene="htpG"
                     /locus_tag="PK049_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HOK60176.1"
                     /translation="MQTGKIGVTTENIFPIIKKFLYSDHEIFLRELISNAVDATQKLK
                     TLASVGDFKGELGDLTIRVKVDRKKGTLTISDSGVGMTADEIDRYINQIAFSGAEEFL
                     EKYKAQANGIIGHFGLGFYSSFMVSDKVEIITRSWQDGAKPVRWSCDGTPEYKIEDAD
                     REARGTDIVLHISDDSKEFLEDHRIEELLRKYCRFLPIPIAFGTEKEWKDGKEVDTGK
                     DRIINETNPLWTRKPAELKDEDYQKFYRDLYPAQEEPLFYIHLNVDYPFNLTGILYFP
                     RIKTNFDIQKNKIQLYCNQVYVTDSVEGIVPEFLTLLHGVIDSPDIPLNVSRSYLQSD
                     SNVKKISSHITKKVADRLQEIFKNDREQFEKKWDDLKLFITFGMITDEKFYERAEKFA
                     LLKNVDGKYFTFEEYEKLVKENQTDKNKTLVYLYASDKDDQHSYIEEAKARGYDVLLM
                     DGQLDPHFINTLEAKLKDTRFARVDSDVIDKLIVKEDRKEVSLPADEQADLRLVFEGA
                     LPAQDHYWVSFEELGEGANPVVITQSEFMRRMKDMSAMSPTSSFYGSMPDSRNLVVNV
                     SHPLVKSIIEAKSKQLSERLNEVNEKIKPVKAKVEELEKAQKGKKDDEIPQADKDALA
                     DAEKQLDKLEEERSTVLKDFGKNYKLVKQLIDLALLANNMLKGEDLSRFVKRSIELIK
                     PE"
     gene            complement(63436..63966)
                     /locus_tag="PK049_01070"
     CDS             complement(63436..63966)
                     /locus_tag="PK049_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60177.1"
                     /translation="MDTLDKIRRKIAIYIVDYSIEWSDILFNGLECSEPFYIKKFANG
                     EEFINYLKTEKLSKRITHVALIGYTFYDEKNQTLMNGIEILESLKKIKPFVNAIMLAN
                     EDEQEYGGYVKKLGAVAVINKDDSALLRINNHIALLFSKQALAQRKKDFKIALYIWIG
                     TIIIMASYLLIGYYLI"
     gene            complement(64642..66663)
                     /locus_tag="PK049_01075"
     CDS             complement(64642..66663)
                     /locus_tag="PK049_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOK60178.1"
                     /translation="MTLICFATAGFAISSCDSGKKNDDFKWQIDQFADIRIMRYQIPE
                     FEKLTPQQRVLVYYLSEAAKCGRDIIWDQNYKHNLAVRSILEALYKGYPGERNGEQWN
                     QFMVYLKRVWFSNGIHHHYSNDKIMPGFSAGYFTQLVNQTPDSLFPSELGGKVQIIEK
                     YLPIIFDPNVAPKRVNQEAGVDMVAQSACNYYEGVTQAEAEKFYASLANPADSTPISY
                     GLNSKLVKENGKIVEKVWHLGGMYGKAIERIVYWLEKAATVAENDHQRQVIEKLIDYY
                     KTGDLKKFDEYNVLWVNDLQSRVDFVNGFIENYGDPLGYKASWESVVNFRNDEATRRT
                     QTISGNAQWFEDHSPIDDQFKKKEVKGVTAKVITVAQLGGDCYPSTPIGINLPNADWI
                     RKVHGSKSVTMDNITYAYDQAALGNGFLEEFAWSAQEVELNKKYGYLASSLHTDLHEC
                     LGHGSGQLADGVKGDELKNYGSPLEEARADLFALYYIMDPKMVELGLIPNLDVAKVQY
                     SNYIRNGLMTQLTRVELGKNIEQAHMRNRQLISKWCYEQGKADNVIERKERDGKVYFV
                     VNDFEKLRQLFGQLLKEIQRIKSTGDFEAGKALVENYAVKVDFDLHKQVLDRFAKLNI
                     APYGGFINPRIIPVTQGDSVVDALVEYPDSYVEQMLEYSKRYSYLPLYN"
     gene            complement(66746..67486)
                     /locus_tag="PK049_01080"
     CDS             complement(66746..67486)
                     /locus_tag="PK049_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60179.1"
                     /translation="MLRSTLEVQSSYTYPFDSILIGKLKSPDGRFRIFTWNIPQPGGY
                     QKYFGLLQVKKADGNVALIELKDDRKSITDVKNEKLPAERWMAALYYQLAQFPTSNGN
                     AYVMLGYDFNNLFTSKKIIDVLTFDSQGTPIFGSPVFNVDGKYRLSRIVFEFNARVNM
                     VLRYNDDSKTIVFDHLSPSSPEFAGDFQYYGPDGSFDGFKLEGTEWVYVRDLDLRNPR
                     RSKPKPVNAPVDIKEPGFLYKPDSSPKK"
     gene            complement(67665..68855)
                     /locus_tag="PK049_01085"
     CDS             complement(67665..68855)
                     /locus_tag="PK049_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOK60180.1"
                     /translation="MNVLRQLFLEHLAQTSESPLMLEINKADGVFLFGPDGKKYFDLI
                     SGISVSNLGHGNPMVVKAVQSQAADFMHLMVYGEYIQKPQVELTAQLTRLLPENLNSV
                     YLVNSGSEAVEGALKLAKRFTGRSEIVAFKNAYHGSTHGALSVMGCEEQKRNYRPLLP
                     DVRLLNFNSIADLDHITTRTACVIIEPIQGEGGIRLPNVDYLRKLKQRCSETGALLIF
                     DEIQTGMGRTGSMFAFQRFEVIPDILLLAKAFGGGMPLGAFISSKEIMRVLTHNPILG
                     HITTFGGHPVSCAAAIASLNFLMHEKVVEQVEHKGSILAEALAKHPKVREVRRAGLMM
                     AVELGTFDRVQALIKYGLDHGFILDWFLFCNTAVRIAPPLIITEDECRIVANLIVEGL
                     NAIQ"
     gene            complement(68862..69575)
                     /locus_tag="PK049_01090"
     CDS             complement(68862..69575)
                     /locus_tag="PK049_01090"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOK60181.1"
                     /translation="MNCILVDDDEMSRRLIEDYASRVDFINLVRSFSNPIEALSFLQN
                     QNDDVHLVFLDIEMPEMSGIDFLETSSAKPQVVIISSKEKYALKAFDYSVTDYLLKPI
                     TFPRFFKAVSRSYERYNQLRSPFDDDREIFIKKNNSLVRVRYGDILWIEAMENYVIIV
                     TSHERFTIHLTMKSIENQLPSVRFKRVHRSFIVNVGKIETIEDNVISIRSNEGRKTIP
                     IGKSYRESLINELNLIANK"
     gene            complement(69638..70348)
                     /locus_tag="PK049_01095"
     CDS             complement(69638..70348)
                     /locus_tag="PK049_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOK60182.1"
                     /translation="MKLNIDPTDKELRKRLIDYLSQFIYDRRYQLFRSIISNRTRYIT
                     VVLEDIYQAQNASATLRTCDCLGVQDIHIIENSNKFSVDSEVDMGASKWLTLHRYNKL
                     ENNTLEAINALREKGYRIVATTPHDNDCTISELDLSKGKVALLFGTELTGLSDLALKN
                     ADEYVRIPMFGFTESYNISVSVAITLYELTNRIRNSEIDYRLSSDEQDELMLQWLRAS
                     IRSSSLIEKRFLNELKMH"
     gene            complement(70410..70682)
                     /locus_tag="PK049_01100"
     CDS             complement(70410..70682)
                     /locus_tag="PK049_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOK60183.1"
                     /translation="MNKAQLIDAIAAEAKITKADAKKALDAFIKTTGTALKKGDRVAL
                     VGFGSFSVVKRNARTGRNPQTGKPINIKAKKVVKFKAGSELSGKVN"
     gene            70937..71026
                     /locus_tag="PK049_01105"
     tRNA            70937..71026
                     /locus_tag="PK049_01105"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:70971..70973,aa:Ser,seq:tga)
     gene            complement(71057..71272)
                     /locus_tag="PK049_01110"
     CDS             complement(71057..71272)
                     /locus_tag="PK049_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6132 family protein"
                     /protein_id="HOK60184.1"
                     /translation="MNLKTKVDHFYQWFKEKKRVTYPVLAVAIGIVAGYLYYYYIGCN
                     SGSCPITSSPWSSMGIGVLMGWLLVSK"
     gene            71390..71481
                     /locus_tag="PK049_01115"
     tRNA            71390..71481
                     /locus_tag="PK049_01115"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:71424..71426,aa:Ser,seq:cga)
     assembly_gap    71814..71913
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(72160..>73127)
                     /locus_tag="PK049_01120"
     CDS             complement(72160..>73127)
                     /locus_tag="PK049_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOK60185.1"
                     /translation="VVVLVLWYGGNLIFAGKGTLGPESLIAYIGIFYMIINPAKSFSN
                     AYYNVLKGLASADRVDTILNAVNPIKVSAGAKIIESFNHDIEFRNVSFKYQDDWVLKN
                     INLTIKKGMTVALVGQSGSGKSTMVDLLPRFWDVTEGEIFIDGVNIKDYNITSLRNLM
                     GNVNQDPILFNDTFYNNIAFGVDGAKMEDVVNAAKIANAHDFIIATPNGYHTNVGDRG
                     SKLSGGQRQRVSIARAILKNPPIMILDEATSALDTESERLVQDAIDNLMKHRTSIVIA
                     HRLSTIRKADLICVLHEGEIVERGKHDELLKLNGYYARLHSMQLL"
     assembly_gap    73128..73227
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<73228..73577)
                     /locus_tag="PK049_01125"
     CDS             complement(<73228..73577)
                     /locus_tag="PK049_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60186.1"
                     /translation="MKKFIRILKYLYPFKWYVVINVFTNVLAAVFSLFSLAMLIPFLQ
                     LLFGTVPLVEVKPEFVISAKGLAQYLNYYLSEIIKTHDRASALLFVIVLVLVASFLKN
                     LFTYLSLYYLAPIRT"
     gene            complement(73661..75007)
                     /gene="purB"
                     /locus_tag="PK049_01130"
     CDS             complement(73661..75007)
                     /gene="purB"
                     /locus_tag="PK049_01130"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOK60187.1"
                     /translation="MLNHSLHSISPIDGRYAKKTESLSGYFSEFALIKYRVWVELEYF
                     IALCEIPLPQLQGFDKSKFTILREIYKSFGESDALRVKEIEKVTNHDVKAVEYFLRER
                     FDAYGLESYKEFIHFGLTSQDVNNTAFPAMLRDSLKTEYLPLLEKLLAKLKELSTQWA
                     DIPMLAHTHGQPASPTRLGKEFRVFVERIEVQLEQLKHIPYSAKFGGATGNFNAHIAA
                     YPSIDWVGFANQFVNYKLGLHRSQTTTQIEHYDNLAAMFDCLRRINSILIDFSRDVWM
                     YISMEYFKQKIKEGEVGSSAMPHKVNPIDFENAEGNLGLANALFDHMANKLPISRLQR
                     DLTDSTVIRNIGVPIAHSFIALKSILNGLDKLIVNTDAINRDLQSNWMVIAEGIQTIL
                     RREGYPNPYDALKLLTRTNQQVTSDTFTNFVNSLNVKDSVKRELLELTPFTYLGIK"
     gene            75131..>75423
                     /locus_tag="PK049_01135"
     CDS             75131..>75423
                     /locus_tag="PK049_01135"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOK60188.1"
                     /translation="MKKYKVSAISYLNTAPFAYGLKHSPVFNKIELLYDYPAECARKL
                     ANNQVDLSLLPVGALSDFSSFDIVSNYCIGSIGKVRSVALMSNSNLSDIKRIYL"
BASE COUNT        22851 a        15628 c        14905 g        21739 t
ORIGIN      
        1 ttatttttgt ccatacacat aagtttgtgt agacattttt caggacgggt catttgatgc
       61 gggaatgagt cggaatgaat cggtatgatt cggaataggc agaatatgat ggagctagag
      121 gaatttagag tccgtgaaac gtgaaccgtg agtcgtgaaa cgaaagattt gatgcgggaa
      181 tgagtcggaa tgaatcggta tgattcggaa taggcagaat atgatggagc tagaggaatt
      241 tagagtccgt gaaacgtgaa ccgtgagtcg tgaaacgaaa gatttgatgc gggaatgagt
      301 cggaatgaat cggtatgatt cggaataggc agaatatgat agagttagag gaagttagag
      361 gaagttagag gaatagcaaa aagtgaacgg ctaaaccaag ttatgatgaa ttaacaaata
      421 caaccgggct aataaaaatt gtggatacat aaaatttatt agaggaaatt tatcaattga
      481 aacaagctgt ttaaatgttt tattgtgttt ctattgatta atctgggttt aaaattggaa
      541 tggttaaccg ttagtgctta ttttttcaag catctccttt ctaagaatga ttaacttttt
      601 accggaagtt atttcaataa tacctaattt gattagctcg gaaaattgtc ggctaacgct
      661 ttcgcgcgaa gcgctaacaa agttggctaa ctccgttatg tttaagggga gttcaaaggt
      721 atttgattta aaaagatggt ttgagaaaca gaggagcaac tcggcaattc ggccaggtaa
      781 ttgcttctgc gacagattag taaatcgctg aaaatcggct aattcgctcc ggcataaatc
      841 catgattatt tcataagcaa agtcgccatt ggagaaaata aattttttaa aggcatcgag
      901 ggaaataaag caaacctctg aatcaactaa ggttgtggca gagtaatggt taaccttatc
      961 gccaaaggtt gtaggtaagc caaggtatgc aggagctttg ttcaacctta atatcttttc
     1021 gccaataggg cctttcatat gaatttttac taaccccgat cgcaagtaaa caatattggt
     1081 tgcaaaggtt ccctcgcgaa aaattgtttc gccttttgca aattttacct gggcacagtt
     1141 ctgttctagg gtatcaatct cattgggcct aagcagttca gccgccggag atttcatctt
     1201 acaaagcttg caatcgggtt tcataaaaac attttttcaa atatagtcag aaagcgtgac
     1261 ttatatcaca tggttataaa atatatctca caccattttt tatttaatcc attgcattac
     1321 aacctacttt tggattgtta cttcgataac agataattaa ccaaatacct actcaatatg
     1381 gaagaagaaa tgctcaaaca gcttgaagac ctaaaaaaaa gggttagtaa gctagagaaa
     1441 aatcgtaaag atcagctgtc gatagcgata gtatcgggcg acctggacaa gattttagcc
     1501 actatgatta tttcgcttgc ggctgccgcc agcgatacta aagttaagct gtttttctct
     1561 ttctgggcgc tttcagccct cagagatcaa aagaaaaaag ccaaagggaa gagtttcatt
     1621 tccaaaatgt ttggacttat gctgcccaaa gggcgcaata agcttaaact ctcaaaaatg
     1681 aatatgatgg gaatgggacc tgccatgatc aagttcctta tgcggaaaca gaatgttatg
     1741 tcgctcgacg agatgttcaa acaggcagct gaactcggta tagagattgt tgtttgcgaa
     1801 atgtcaatga acctaatggg tttcaaaaag gaagaaataa ttgattaccc tcacctaagc
     1861 tatgccggag cggcaacatt tgtagcagag gcaagtcaaa gtagcattca atttttcatt
     1921 taaaatacca tcctattatg agccagaaaa tagttataat agtaacccat ggccccgaca
     1981 accaagaaaa agcaacattg ccatttgtaa tggcaactgc tgcacaagcc atggatgttg
     2041 aagtaacaat aatactccaa agtgccggtg ttttacttgc caagaaaggg ggatgcattg
     2101 aaaatattca tgcaccagga cttatgcctc ttaaggaatt attagatacc ttcttgagct
     2161 taaatggcaa tttactccta tgcacaccct gcgtaaagga aagagccatt aaggaagatc
     2221 aactcattga tggatcaaag ttaatagcag cgggaacggt aattactgaa acgttatcgg
     2281 cagatgccgt attaacatat taataaccaa atataaattg ttatgaacgt ggaagaacta
     2341 aagaaactta gtgtggcaca aacagttgat gcacgtggaa cttcgtgtcc tgggccactg
     2401 cttgctgcaa aaaaggcagt aggtgaaatt gaatcgggcg aaattcttga agtgctatca
     2461 tccgatgagg gtaccaaaaa cgatatcccc aaatggtgcg aaaaaatgga acatgagttt
     2521 ttaggtatca ttgaagagga tagctacttc cgcctatttt taaaaaaggc ttaaaacaaa
     2581 ttggcgcaaa aggtatgtaa tccttttgcg cctttacctt taaaaaaaat aatatgaaaa
     2641 ccgaaggcta cgcaccaaga atactggtat tctcaaccga taaaatttcg gacccgggaa
     2701 ttgatcaggc tgggttgaga aaactacact attcaccaag cgtctatgtt attagtcttc
     2761 cctgctcttc tggggttaaa ccacgctgga taatgaaagc ctttgaggaa gggttcgatg
     2821 gtgtttttat agctgccgac gggcatgaat gctcttacag cccgaaatgt gccgaacaca
     2881 ccaacaatat tatagttgag gcccagcggc ttatgaggga acgaaatatc aacaccaaac
     2941 gaatccgtat ggcagccatt tgctcagttt gtgctgaacc atttgtaaac catatgggaa
     3001 cattctcaaa aattttagcg gaactaggta gtgttaaaaa tgaaattgca ggtaattctt
     3061 agcatttgct aagccaaata ataaatcaaa taaggcaaaa atgacaacca ataataaata
     3121 cgatgcattg gtaattgggg gcggaattgc cggccaggag gccgctctta atcttgccga
     3181 catggattac aaggttctgc tggttgaaaa agaactttcc attggcggta aaatgataca
     3241 gctgagcaag gtattcccca ccctcgactg cgcagcctgc atcaccacgc caaaaatgtc
     3301 cgaaacggct cggcatggta atataaaggt gatgttaaac agcaccgtaa aagaaatcca
     3361 aaagcacaac ggtgctttta cggtgaaagt aaatcatggt gcccgttatg ttaaaccgga
     3421 agcctgcaca ggttgtcagc aatgcgaatt tgcatgtcct gaggttagac ccgatgatta
     3481 caatacccat ttggcaggcc gaaaggttgc ttacattcca tttagcttag caaatccccg
     3541 tattgcagca attgaccgga atggaacatc ggcaccttgc ataagtgcat gcccgggcgg
     3601 tgtaaaacca tatgggtatg tgtcgttggt gcgaaatggt cagtatgaag aagccatgaa
     3661 tcttcacctt gaagatattc ctctaccagg gagccttggc cgtgcctgct atgctccctg
     3721 ccagggtgaa tgcaccagag gcaaccttga aggtgctgtt gatatccgac gtatcaagcg
     3781 tttctttgcc gagcaatact acaacaaatt ccccgaaccc ccgactcgtg aaattaatgc
     3841 aaacacaaac aaaagggtag ccattattgg ttcgggacca gcaggattaa ctgcagcata
     3901 ccatcttgcc ctgaaaggcc ataaagttaa aatttttgag gcagcaccta aggctggagg
     3961 tatgcttatg ctcactcttc ctgagtatcg attacccaaa acagtagttg aaagggatat
     4021 taagaatatt acatcgcttg gcgttgaaat tgagtgcaat aaaagggttg agaatattac
     4081 cgaattgaaa cagcaaggtt tcgatgcagt atttgttgca gttggaactc accaaagtag
     4141 caaaatgcat attgagggtg aaaaccttga aggagttgaa gggtgtttag actttcttag
     4201 aaatgtaaat attggcgaaa ggtataacct taccggaaaa agggtaatag tagttggggg
     4261 aggaaacact gccattgatg catcgcgaac tgccctgagg cttggagcag aacaggtaac
     4321 ggttgtttac cgccgtagcc gtgaggaaat gccctgcttt gcccccgaaa taaaagaggc
     4381 agaagaagaa ggcgtcaaaa ttgatattct ttgcaaccca attcggttta taggacaaaa
     4441 tgggaaggta agcaaggttg aactggttag gatgaaactt ggcgaacccg acgcaagcgg
     4501 ccgtaggagt cccattccta ttgaaggttc caactatttg gttaatgccg atttggttat
     4561 tgaggcagtt gggttacaac catcaaccca accatttgca aacgaattgg agctggccaa
     4621 aggaggaacc ataaaagtaa acggcaaaac ctttcaaact aacatcccct atatttttgc
     4681 cggaggcgac agcgtaagcg gtgcttcaac cataattgaa gcagcaggac agggtaaaaa
     4741 agcagcattc tatatcgaca agtacttaaa tggacttgat atcgatacct ttgaatttga
     4801 tgagaaactt cctgccgctg acaagaacga ggttattgcc aagcgacacc ccgcactaaa
     4861 aaaacctgta aacgatactt ataggaaacc ggctgaaagg gttaaggatt ttaatgatgt
     4921 tgagcatacc cttacagagc agcaagccaa ggagagcgca gagcgctgtt tggattgtag
     4981 taattgtcgc gaatgccacc aatgtaaaac ggcatgccct gccgatgcaa ttgattttgg
     5041 ccaaaaggat gaactgatta cagttgatgc caaaacggta attgtttcaa ccgggtttaa
     5101 gctctttaaa cccgagcaca aaccgcaata cgggtatggc atttatccta acgtgattga
     5161 tgctatgcaa atggataggc tcatagcgcc tacccgcccc tttaacaatg ttttgcgccc
     5221 gggcgacggt aaaatacccg acaagatagc ctatattctg tgcaccggtt caagggattc
     5281 cagccttgag aatgctatgg cttgtagtgg cgaatgctcc aataacccaa tatgctccca
     5341 aatatgctgc atgtattcca taaaacaagc ccaactcctt atgggtgctt tgccaatggc
     5401 cgatatcacc atatactaca tggatatccg agcatttgga aaaggttacg atgaattctt
     5461 ccaggaagcc aaatcgatgg gagtaaactt tgtcaagggt cgaattgcaa aaatattccc
     5521 aaaagaccaa cccaatggcg atttaatact ccgctatgaa gatgtaacaa ccggaaagtt
     5581 aaaggaaagc aaacacgacc ttgttgtcct ttccgttggt gttctgccca atagttccat
     5641 ttccagggta ttcagcaatg caacccttga gctcgacgag caaaaattca tcaagcagat
     5701 agatgaattg gtaaacccag cccaaacaag cattccgggt gtatttgttg ccggaaccgc
     5761 ttcagggcct aaggatatcc ccgattctat tctatcggca ggaactgctg ctgctgaagc
     5821 cgcaagctat ataaacacgt taacacatta aaccaaccac atcgaaacat cagcaaacca
     5881 tgaagcaacg aataggagtt tacatatgcc actgcggcgg aaatatatcc gattatgtgg
     5941 acgttgaaca gctgagcaaa cttatagcaa acgagaataa cgttgttatc tccaaggatg
     6001 ttatgtttgc ctgttccgac tccaaccaaa aggagatgat acaggacata caggaaaaac
     6061 aactcgatgc aattgtggtt gcatcatgtt caccaaaact tcatacccat actttccgga
     6121 atgtggcgct aagagcagga ataaacccct acaactatgt tcaggtaaat gttcgcgagc
     6181 agtgctcatg ggctcactcc gataccccac ttgacgcaac cgttaaggct tacgggctaa
     6241 tcagagctgg tatcaatagg gttgcctact ctgaagcact tgataatatt gaaataaaag
     6301 cgcaaaaagc tgttgctgta ataggtgcag gagtagctgg catgcgcgcc gctattgagc
     6361 ttgcacggtt agggaacaac gtttatctta ttgaaaagga agaacaagtg ggtggaaggg
     6421 ttgcccaaaa tggtaaactt tttatgactg gcgaagatgg caaagctctt actcaaagac
     6481 tattagacaa tgtaaagagc tatagccaga taaacctctt cacaaaggca acactcgaaa
     6541 aagtttcggg aagcgtaggt aattttaaca tagatgttaa tgttgaaggg aatcttttaa
     6601 aacttaacgt tggagcaata ctcgtaacaa ccggtttcga ttactatcaa ccctccgaaa
     6661 atgaatacgg ttttggacaa accaataggg ttatcaccct tcaggaattt aacaaacttg
     6721 ttgaaaataa atctaaagag ttaagctttg atgataaacc agtaaatagc attgcctata
     6781 tatactgtgt tgggaacaga caatcaaaag gtccaaacag gtattgttcc cgttactgct
     6841 gcacagccgc cattcactca tcaattacag cccacgaaaa gttttccgga ataaaagcct
     6901 tccattttta tcgcgatatc agaacgtatg gtaaacagga attactttac cggcaatcat
     6961 cgttaaacgg ggatgtatac cttaaattcg aggaaaaaga accgcctgta attgaagttg
     7021 aaaatggtta cccagtagta aaagtaaagg attaccttac atcaaggaaa gaaattgcgt
     7081 taaaagctga tttggtagtc ctagtaacag gcatggttgc acgttccgat agcaatagtg
     7141 tttcggacaa gctaaaaatt ccggtaggaa acgataagtt tttcaatgag attcatccta
     7201 agctccgtcc ggtagaaacc gtaataaatg gtgtgtacat cggtggcgcc tgtcaggggc
     7261 caaaaaatat taccgagtct gtgcaatcag cacttgcagg tgtttcaaaa attaacgctt
     7321 tgctcaaaaa agaggtaatt gaactggagc caattgtggc aagagtaaat aaagaaacct
     7381 gcttgtggtg cggtaaatgt gccgaggttt gcgagtatga tgccataaaa ccccttgagg
     7441 taaatggtaa aatggttgct gaagtaaatg tagctacctg caagggttgt ggcatttgtg
     7501 cacccgtttg cccaaacgat gccattgaga tagcagagta ttcaaatcac ggtattgaat
     7561 cgatgattgc aggttttgca gctcaagctg aaatcaatca gactgaaacc caacacgagg
     7621 aaaaagatga aaacgccctt gtgggcatga aggaataccc aaaggaatgg aaaaccatac
     7681 ttgaaagttt taatggcacc ccactatcaa ttccacaaat atccaatatg tcgggtcttg
     7741 ccaaggaatt ggtaacctat cacctaatga ccatgaacag gtatggcata gttgaacctg
     7801 ctgggttaaa cgatgaagaa tcgtactatt tgtataaacc taaaaatcac tagttatgcc
     7861 cactataaac ccaaactttg ctgaggaaat taaaaaattc ggagcaaaag attttaatct
     7921 ttgttttaac tgtggaaact gcacagccat ttgctcactt tcaacaaacg atgaaacttt
     7981 tcctcgtgaa ctaattcgtt acagcacgct tggacttgag gatgacctaa aatcatcgct
     8041 caaaccttgg ctatgttact attgtggcga gtgtagcacc gattgcccca ggcaagccaa
     8101 tcctggagaa ctgatgatgt cgcttaggcg ttggctaact gcccagtacg attggacagg
     8161 gttatcaggt ttgctttata aaaacctctg ggcctacatt ctagcaatga tagccatttt
     8221 tggcgcagtt gtgggaattt catacttttc agaatttacc cattccgagc tgatgcacat
     8281 tggccatctt tttgagatgg ttgccatagc cggagttttc gcaattatac tcctgccaaa
     8341 cataatcagg atgtggtatt ttaccattat taaacctgga ataaaagtaa atctaaaagc
     8401 ttattttctt tccttaaagg acctattaat tcacatgttc acccaaaaac gattcctggg
     8461 atgtgagccc acccgcgaga ataaaatgcg ctggttgctt catcttattc ttgttattgg
     8521 ttacctctca ctacttttca caacggtctt tctaaactgg ttttccacta caagtaattt
     8581 catagtatgg ctgggttaca ttgagagtgc agcaatattt ttaattacaa ttctgttcat
     8641 gcaaaaacga atgcgtaagg ttgatgccat gcacaagcac tcccaaccat cggattggtt
     8701 ctttgtaatt tggttattcc tcatgggttt tacagcattt atggttcgtc ttttaattga
     8761 cacccagcaa atagatagtt tcttttgggt ttatgtcatt catattactg tgcttgctca
     8821 gtgggcatta ataatagtgc catttggtaa atggactcac tttttatata ggtcctttgc
     8881 aatgtatttt gatgcactta ttatggccaa ggagaaaaaa tagtgttact atgaacgaaa
     8941 aaggccggca acgtccggcc tttttattat acctctttgt caaagaaaag cttgtagtag
     9001 tttagtcccg tatcggggtc aaagccgcgc tccacgcggt cgcgcccccc atgaatgtat
     9061 atgtgaaaat tcttatcgag tttcactacg cttttaaaaa aattttgcga ttgccgggtg
     9121 gcatcggttg atatcttgaa actatcaggg atattacagc caattgcttc ctcatacttt
     9181 tgcttaaaat cgcggaaggt ctcagccatc ttgggctcct ccagaaccac ctcttcaaac
     9241 tcctttaggt caaaaacctc gtttgcctta aaaaaatcac gcgttttaca aagaatatca
     9301 gcctggtcaa cttttacaaa gttgttctca ggtttaacta cctcctccac aaactcacgg
     9361 caaagattaa ttgcctgctt ggtttggtaa aaatcgtcgt tacgaagttt ggcacgcaga
     9421 aaatcattta gccagtactt ggcttcgtcg cggttggtat tatcaattac gcaaagttta
     9481 tagccaaaat ccttttcggt gttaaaaact aagcaagcct tatcgagctt gcggggtgta
     9541 gtgccaaact gacaagttac cttcaatcct ttggcatcgc ggcttacatg taaaaaacgc
     9601 tccttgctct cggccttaaa aattccaatg ccatcagaaa gttcaccatc aacaaccata
     9661 tcggtaaaag aggcaatgta aagctcccct cccttaatgt taggatgaac tgatacatgc
     9721 tgaaggtgat tggccacatt ttttgaaaac tcaataaagc taatattccc ctcaaaaaaa
     9781 tcgctgcagt aactatacaa ttcattaagt tcaaggcttg aactgtgctc aaatgagtaa
     9841 aatccttgat tcctaaaact ccctagaaag tactccctaa gcaggctatt caataggtta
     9901 tcatcaatat ctaaaggtgt atctgataaa ataatatcat cagtatctac tccaactgag
     9961 tggactataa cctcggttag ttcagcattt gaaaagtcaa gcatggtaat tttaaattaa
    10021 tttcacgaaa ttatggattt ggaatcacct atgcaaaagc tataacaact aataatttag
    10081 acaccataaa aattactttt ataagtaatt aacattttac cacttagcta atagggtaat
    10141 tgggaaattg taaaaaattg ctaagggttc taaaaaaaat ataactttgc ttgttaacaa
    10201 actaacaatc aacccagaaa gccatgggta aactttttga caggttaaac tccgatgttc
    10261 gctttgttga gggaataaac ggatgcataa actgtggtac ctgcacagca atatgtcctg
    10321 cagctgaggt ttacgactat gaaccccgaa ccatagcaaa cctcgtccaa atgcgcgacg
    10381 ataatgttat tgaggaatta cttaaaagcg ataccatttg gtattgcggc gagtgcatga
    10441 gctgtaaaac acgatgtcca cgaaacaatg cacccggctt actgatacaa gcgctaagag
    10501 gattatccat agaaacaggg ctttttgttg aatcggaaaa gggacgtcaa cagctttcgc
    10561 taaagcggat gttgggcaac tccattctgg aaacaggcta ctgtgttcat tttgaccacg
    10621 cagaattgag catgttccca gaactgggcc ccgtttggca atgggttagg gataaccgtg
    10681 aaaaggtttt agcaaaagta ggggctaact acaatggttc ggggcctggc gccatgcgca
    10741 aaatctcaaa ggaaaacctc gacgagcttg aaaagatatt tgaagttacc ggcggcttgg
    10801 attggtttga taaaattgag gacgtatcga cacaaaaagc caaagaacta ggcatggaaa
    10861 agggtgatgc taccgataaa aaatcggcct actttaccca tgtttatagg tataacaatg
    10921 ctgaaaagca tcgccgtaag tagtttcaaa aaaggataaa ttcaatgaaa caggaaggta
    10981 aaaaacgtat atggagcgat taccaaaagg aaattgctga tgataaattc tactttgtca
    11041 ggagttgtat aaggcaaacc ttcttcccgg gtagcgattc cacctttatc agaattttac
    11101 gggatgtgct tggtaaagat atttatgaga accctgacca taccacctgt accggcattg
    11161 gttaccatac ggaggtagta ccatttccaa ccattcaaac tgttgttgcc cgtaattttg
    11221 ctctgatgcg tgaaaagggt tacgttaact ataccccatc gtgcgtaacc tcctttggta
    11281 tttacaccga gatacttgaa acatggcatc actttccaca ggaaaaggaa aaaaccagag
    11341 agtatttaag aaaagcaaca ggacgggagt ttgaagagcc tgacaattta gctcatacaa
    11401 gtgatgtgct gtataaattc cgcaacgaga ttgcagcaaa agctaagtat agattaataa
    11461 acaagcacac tggaaaaccg cttaaagttg ttgaccacat cggctgtcac tatgctaaaa
    11521 tgtttccaag caagggggtt ggtggagctg aatatccaca ggtacttgcc ggactggtcg
    11581 aagcctttgg tggtgaggta attgactacc cggaacgtag gcactgctgt ggttttggct
    11641 ttaggcaata ctttgtgcag gccaatcgag gttattccat atcggcatcg aaaaagaaat
    11701 ttgagtccat ggaacccttt cagcccgatt ttattataac taattgccct ggctgcaact
    11761 actttatgga taggtggcag tacgcccttg gcgaaatgga aggaaaaacc tacgggcaaa
    11821 acggcgaagg tattccagtg ctcacctatg aagaggttgc cggtttggtg cttggcttcg
    11881 acccctggga aatgggcatg caaatgcacc agaccgatgt agaacccttg ttaaagaaaa
    11941 tgggtgttga gtacgatccc aaccaaaagt ataaggggcc caacggcgaa gatcttggcg
    12001 taccaatgag tccccgtttt gtgaatgaaa aggcataatt aaaatatgaa aatatcagta
    12061 ctcccatgaa aaaagtagca gtaataggag gtggcgttac agggcttgaa gcagcaaacc
    12121 agcttgctca aatgggttat tctgtaacag tttttgagaa aaaaaacgct cctggaggcc
    12181 aggtatcaaa gtgggacaga cttttcccaa accgtaggca ctctattgat gtggtgaacg
    12241 aactaaaatc gcatcttaat ggaaacccgg ccatctacct atcaaccgca ataaataaaa
    12301 ttaccaaaaa agatgaacgc tttcagcttg aagcctataa ccggattttt gaagccgatg
    12361 ctgtactcat agcaacagga ttcgaaccat tccctgctga gcgcaaggag gaatacggct
    12421 acggaattta cgacaacgtg ataacctcgg cagagctgga aggtatgttc aaggagcatg
    12481 ggcaccctat tatgcgaaat ggaaatgctc ctaaaagaat tgctttcatt cactgcgtgg
    12541 gcagtcgcga cgaaaaagtg aagcgccctt actgctcaaa ggtttgttgc gttactgctg
    12601 taaagcaggc aattgaggtg aaagaggcca ttcccgaagc ggaaatattt tgcctttaca
    12661 tggatttgcg gatgtttggt ccgggttacg aggatttata caaggaagcg caacagatag
    12721 gcataaactt tatccgggga cgcttatcgg aggctagtga gctcgatggg ggtagagtcc
    12781 tcataaaaac agaggatacg ctctcctcta aacccctaaa aatgtcggtc gacatggtag
    12841 tgctaatggt agggatgtcg ccagctaagg gaactgatga actaattgca gccctgggac
    12901 ttcaaaccaa caaggacgga tttgttgaaa taactgacta tcacctaaac cctgttaaaa
    12961 ccaaggttag cggcgtattt acagcaggtg cttgcacggg gccaaacaat ataaccgatt
    13021 ccgttaatgc tgcccgttcg gctgccatcg aaattcataa ttacctaaac agcaagcagt
    13081 atgattgatt ttggttttgc cccaatgcca atcagtatcc acaaccttga tactgcaaat
    13141 ttatcgttgg cacaatggat aaaccaacac gatgaaacct ttaaccactg cattgcctgc
    13201 gggagttgta ctgcaacctg taccgccgga caatttacaa ccttcagctt tcgggaatta
    13261 tgccatgcca ttcggagagg cgaggtaaag aacgctattg atgaatcgga aaagtgcatg
    13321 ctttgtggca aatgcactct ggtttgcccg cgaaatgtaa atacccgaaa catcatcatg
    13381 ctaattcgta aagctaacct aacattcagg ccatgaggta ccatccgttc acattaccct
    13441 tttcaattgg gcttggtttt atagttctct acatcgtagt gatgtggact atttggatca
    13501 taaggcttag ccggcgtgag caaaaaatag tttggaaacg gttattctcc ctccgaacag
    13561 tctctgccat ttgggaatcg atacgcgagg ggttactcca tcggaatata ttcaagcgga
    13621 atatactttt gggttacatg cacatgagct tagcttttgg ctggttcatg cttattgtgc
    13681 tcggaaacct tgaggctcgt ttgtaccccc atggggtaat aaatccgcct ttctacccta
    13741 tcttttttga gtttttcagg cacgacctaa ccccactcca tgagtatgaa gagattttca
    13801 cttttctgat ggacttcttc ctgcttatgg tgatatcggg ggttagctta gcctttttta
    13861 aaaggttccg gagtcgcacc ctcggcatga aacgtaccac aaggcatatt ctaatcgata
    13921 ggattgcaat tacggccctt tggttcatct ttccgctaag gctatttgct gaaagcgtaa
    13981 ctgcgggact ttataatacc gggcactttt ttacaaactt tcttggtcag tgggtcatat
    14041 acaatcttcc gctcgattac ctgtactacc ctgcatggtg ggcatactct atagctttag
    14101 gtgttttctt tattactatg cccttttcac ggtacatgca cattcccgct gaaatcctac
    14161 taattttcct tagaaaatat ggcgttaagg aaaaggatac ccactcaacc ttcaccaaga
    14221 tagaattaag tgcatgttcg cgctgcggaa tttgcattga tgcgtgtcag cttagcacct
    14281 cgttgaatat ctccgatact caaccagcat atttcattcg cgatttgcgt tacagccaac
    14341 caagcgaaag gctgagggaa acttgcatga tgtgcatgcg atgccttgag gcatgcccag
    14401 ttggtatcga aataacccga atacgtgaga acgaacgcag ggagtatcca ttattcacat
    14461 cgacgagcta cagctacatc aatggatata aatacccaaa agcaaaggta gcattctttg
    14521 caggatgcat ggggcattta actccatctg taataaagtc gaccctggcc ctgctcgatg
    14581 ccgcaggtgt tgattacacc tttattgaca aaaacggaag tatttgctgt ggtaggccac
    14641 tacaaatgaa cggccaggcc gatgctgcca aacaacttat agccaaaaac aaccatttaa
    14701 ttgttgaaag tggtgccgag gtgctagtaa cctcctgtcc aatatgcgcc cgagagtttg
    14761 ccagtagcta tcaccttaca atacctgttt tgcaccatag ccagtaccta cttcagctaa
    14821 ccaaacaaaa caggttgagg ttcaaccagt cggggttaaa agttacctac cacgacccct
    14881 gtgagctgag tcgtggacta aatattaccc gagagcctcg cgaactcctg caaaatgcag
    14941 cgatcttatc aaacggaagc aaagaaattg aaagcaaatc gttatgctgt ggtggcagcc
    15001 ttggcataac caatatctcc atgaccgacc gttacaaaat tgctaacaac actcttgatg
    15061 aaattgttac tgagagtagt agttacctag taacagcctg tccgatgtgt cgcaaaactt
    15121 ttaccaacgc acaagaaaaa ataaaagtgc ttgatattgc tgaaatattg gaaaagaatt
    15181 taaactttgc acccgaaaaa gcgtcagata aagttcagac gcaagctaaa catcaagcag
    15241 taacaacata aagaaaaaag caatgcataa cgagaaactt ggatttaaca gccttctcat
    15301 tcacgggggc atggagcccg acccaatggg aagcgcaaca gtacccattt accaaacatc
    15361 aacctttgcc tttgagaatg ttgacgaagg agcaaggtgc tttgcaggcg aaagcgatgg
    15421 atttatctac acccgaatag gcaacccaac catttcggca cttgagcgcc aggtggctat
    15481 tctggaaaat ggttttggtg gaattgctac agcctcgggc atgggcgcag tgtcgtcaat
    15541 ttacatggct tttctgaaag caggtgacca cattgttagc accgatgcgg tatacgggcc
    15601 ctcacgcggt attatggaaa cctacttcaa gcggtttggg gttgaaagta cgtatatcga
    15661 cacatcgaaa ctcgaaaaca ttgaggctgc cattaagcca aacacccgaa tgttatacct
    15721 tgagagtccc actaacccca ccatggttat caccgatata aaagcagcct cggagctagc
    15781 ccataagcat gggttagtag tggtggttga taataccttt tgtagccctt acctgcagcg
    15841 tcccctcgat cttggcgccg atgtagtatt tcattcaatg accaagttcc ttaacggtca
    15901 tgccgatatt gttgctggta ttattgtaac taaaacccag gaactctata aaactgttcg
    15961 cccagccatg gtgaatatgg gatgtaatat ggatccgcac caggcctaca tggtacttag
    16021 gggtatcaaa accctttcgc tccgtattga acgcgcccag caaaacgctc aaaaagttgc
    16081 cgaatatctt gagaaacacc ccaaaattga atgggttaag ttcccggggc taaaatcgca
    16141 ccctcagttt gaactggctc aaaagcagat gaagggaccg ggtgccatga taagctttga
    16201 gctaaaaggc ggcattgaag ccggtaaaat actcatgaat aatgtacggt tggccatgct
    16261 ggcggtttca cttggtggag ttgaaacatt aatacaacac ccagcctcta tgactcactc
    16321 aaagatgtcg cctgaagccc gaataaaagc caacatcacc gatggtctag tccgtttttc
    16381 agttggtatt gaggatgttg aagatattat tgctgacctg gatcaagctt tagccaaaat
    16441 ttaatggaaa acccaaaaca atattttacc cgcaattgaa cattgcgggt tttttaatac
    16501 caaaaaaata tataccttta ttttcaattt tcgtaaaaaa atgtatttga acaaaatttt
    16561 acccaatatg aaaaagtaca ttgcgctggt aagttatgtt ctaatgtgtg tttccgttta
    16621 cgcacaggta gagagaatgc cctttggtga taaaatgagc tatactgcca ataagttaac
    16681 cattggaaat tctaaaggcc catcaactcc aggttctgcc ctattttatg aagattttga
    16741 gggtaccacc tatcagctaa ctgataagtg ggaagtacta aggagttcaa cattagactt
    16801 tcagcaaacc acagctgctg caaacccctc atggttcaga tgtacccctc aaagcttcaa
    16861 cggcaatgga tcaacctata tcaagcacgg cgaagcctca ggggcaatat catttactgc
    16921 tcccgatttt acttggctca ttactcacga tactattgct attccatccg atccggaagc
    16981 atttctgaag ttttggcttt ggtattacag caactatgat cagggttaca taacccattt
    17041 ttatgtattg gcatacgatg tagatggcca aacccttgat actctattgt actatggtaa
    17101 tgatcaggcc acatcggtac ccaaccagta taaatcgcta atcaacctat cactttcaaa
    17161 agtagcgggt aaaaatgttc ggttagcctt tgtatatgag aatcggaata accagaataa
    17221 aggaacacaa cttgcaatcg atgatattct agtaaccaat acaaacattc ctgatattga
    17281 attaaaagca attccctaca agtactcaaa agtacctcac ataacttttg actccctttt
    17341 tatcgatctt aaagcaaata tttataacct gggaacagtt ttaaacgata cattgacaat
    17401 ttcagtatcg tgcgataaaa tccccaactt acaatcatct gctgtaatta ccgataccat
    17461 tgaaagtggc gagcagagag tgataactct cagccctaaa gtgaagatta tgcccaccgc
    17521 tgaaagttac actttcaacc ttaatgcttc taccccaccc gataccttaa ctcaaaacaa
    17581 taccgataat acaaccctgg aggtaagtag ttcaatcttt gctaccgata ggggcgcaaa
    17641 aggtggcata tcatttaatc caaacaccca aataggtaac ctctatgagt ttcaacgtga
    17701 tggttttatt gaaggtgttg aaattggttg ggctcaaaac actacaatcc ctgaaaccga
    17761 atatccgatc gattttgtag tgtatatact tgagatcaac ccagctagtc caagtgaagc
    17821 caaaacttta acccaagaat atttcacaaa ggaatactcc gctaatggcg gttcaattat
    17881 ttacttctta cctaagccag cttattgtca taaagggttc tcgtattatg tacttgtaaa
    17941 gcaaacctcc gatactcctc ttggtattgg atacgatggg aatccttacg gagccttttg
    18001 gaaggttgat gaatttttcc cgctgaacgc cacatatatg gccaaccaat ccattggcaa
    18061 catagcggta agagcactcg taagcgagcc tgttgaaaat ccaactgttt tcttcaacat
    18121 taagaatgag caaaaggaac ccgctcaaaa tgttaaggta cgaattgttg agctcgattc
    18181 aacaattaca acaaacgaac aggggcaagc ctccattgag ctaaacaatg gtaaatacac
    18241 ctatagcatc gattccacgg gctattcatc gctcaggaaa gaatttaccg tgttctcgca
    18301 gaatcttatt attaacgatt cgcttgtgaa agcctacaat gtgaagttta ggattgttga
    18361 cactgactcc gtaccactac ctggagctag aattaatgtt taccccatta ccctcacaag
    18421 taatgagaat ggcgaagata gtaccatgtt accttctgga aactactcac ttaaagtaat
    18481 gctttccaat tatatgatga aatacccgat tgatttactg gttgaagata aggataccct
    18541 ttacattgtt gtaatggagc aagctacaac ctacaacctg actgtaaatg taaaaaacac
    18601 taaaggtgaa agcttaccca atgctgatgt tagtttaatg ggctatggaa cccttaaaac
    18661 aaataacgag ggtaactgtg ccttcaacgg actacttccc ggaaacataa tgggttctgt
    18721 taagtattac aattatattt ggggatcaat ttttgtggat cttacctccg atagcactat
    18781 caacgtaacc ctaatccctg agcactatac tgccactttt tacgttacat ccaagggaaa
    18841 tcctattatc aatgccgaaa ttattattga gggtttagac accttacata ccggactaaa
    18901 tggttacgct caatccacat acattccctt cgggaacaac ataccataca cagtaaaagt
    18961 gaaagactac tacacttaca ctggtacctt tgatatttgg gataccgacg ttcttgttac
    19021 tgttaacctg acgcctcttg gagtagatga caattatgat gatctaaatc ctacaattta
    19081 cccaaatccg accaccgatt ttattacagt taccggcatt aataattatt ccatctctat
    19141 ttttgatata aacgggaggg tagtttacca gaatagtaat cctgacagca agattgatat
    19201 tggtttctta ccaaagggga tatacatagt taagcttaat acatcaaaag gttttagtat
    19261 tcataaagtt ctaaagcagt aattctaaaa taatgtagtt aaaaaaggag tgcaaagcac
    19321 tccttttttg ttacattcta taagcataaa atcatttttt atcaatatta ttttaattat
    19381 caatttaaat tgataacaaa ttatcacttt tttttacgtt tttctgaaag actaaaagaa
    19441 atattttgta aacttgtagt gcttaattcg tgaagtgtga actgttgata tgtatctgta
    19501 ataagcaaat tgtcaataca ttataaaaca taccatgaag aatacgccca tcccctacga
    19561 agtagtaaag cgaaagattc aggaatcaaa gttaccaagt gtaggtaagg cctcgattcg
    19621 tgagctggtt aaacttgtca atgagattga aaaggaaacc ggtatcaagt acgtaagaat
    19681 ggaaatggga gtgcccgggc tggagccatc acaaattggc attgatgctg agattgaagc
    19741 attgaagcga ggggttgcct ccaagtaccc tatgattgac ggcgtgccag agctaaaaaa
    19801 ggaaattgca cggtttgtaa aaaacttcat caatattgat gttgatgaac agggatgtat
    19861 tcctacagta ggttccatgc agggtgctat ggctgctttc ctggtggtaa accgttgcaa
    19921 tgcaacaaaa gataccgttc tattcatcga tcccggattc cctgttcaaa aacagcaact
    19981 tagggttctg ggaatgaact acgaaacctt tgatgtatat aacttccgtg gcgataagct
    20041 ccgcgataag cttgaatcgt acctcaaaaa gggcaatatt tcagcaatac tttactcaaa
    20101 tcccaataat ccctcgtgga tatgcttcac cgagaaggaa ttgcaaatta ttggagaact
    20161 agccacaacc tacgacacca ttgttattga ggaccttgct tactttgcca tggacttccg
    20221 caaggatcta tccaaacccg gacagcctcc gttccagcct actgtggcta actatactca
    20281 aaactggtta atgcttattt ccagctccaa ggcattctcc tatgcaggtc agcgcattgg
    20341 cactatggtt attagcaata gtctttacaa gcgtcgttac cctgacctga aacgctactt
    20401 cacttccgat gagtttggct acgccatgct ttacggagct gtttactccc tatcgtcggg
    20461 ggtttgccat tctacacagt tcggttttgc agccatgcta aaagcagcca acgacggaac
    20521 cttcaacttt gtagaatcgg ttcgtgagta cggtgaaaag gccaaaataa tgaaaaagat
    20581 ttttaccgaa aacggtttta caatagttta cgatatggat gaagacaaac ccttggccga
    20641 tggattttat ttcaccatat cgtacccggg tttaaccgga gagcagctaa ttgaagagct
    20701 tctttactac ggagttagcg ctatttcatt agccataacc ggtagtgaga ggactgaggg
    20761 cttacgtgcc tgtgtatcgc aggtgcagcg caaccagttc tccgacctgg aggagcgttt
    20821 aaaacgattt aatcaggatc acaggaacta aacaaccaaa tagtaaaaca acaaaaataa
    20881 atttgaccaa tatggcaaaa gtaagaggag caattgtggt cgatgtagag aaatgtaaag
    20941 gatgtggcct ttgtgtggtt gcctgccctt caaaagttat tgaactggca agggaggtaa
    21001 atggcaaagg atacaactac gcctacatgg ccaaccccga agcctgcaca ggctgcgcaa
    21061 actgcgcatt ggtttgtccc gatacggtta tcactgttta cagggttaaa actgaagctg
    21121 aggcttaaaa accgacattt tgtataaaca aaaactcatt aaagaaaatg gcagagttaa
    21181 aattaatgaa agggaatgag gccatagccg aggcagccat acgttgtggt tgcgatggtt
    21241 actttggcta ccccataacc ccacaatccg aggtgcttga aaccctaatg gctcgtaagc
    21301 cttgggaaga aaccggtatg gttgtcctac aagctgagag cgaagttgcc gctattaaca
    21361 tggtttacgg cggtgctggt tgcggtaaaa aagtcatgac atcatcatca agccccggga
    21421 taagcttgaa acaggaaggt attacctaca ttgccggagc tgaactacct tgcttaattg
    21481 ttaacgtagt acgtggaggt cccgggctgg gaaccattca gcctgcccag agcgactact
    21541 tccaggcagt taagggtggc ggccatggcg actataagct aattgttctt gctccagcat
    21601 cggttcagga gatgtacgat tttgttgacc ttggatttga actcgctttc aagtaccgta
    21661 atccagccat gattctttcc gatggtatca tcggccagat gatggaaaag gttcaaattg
    21721 gcccctataa accccgcatg accgacgagg aaatagtagc caagtatggt tcatgggcca
    21781 caaccggtaa aacccctaac cgccaacgca atatcatcac ctcgcttgac ctcgatgcag
    21841 gccgtcagga gcagttcaac ctaaaacttc agcgcaagta caaagagatt gaggaaaaag
    21901 aggttaggtt tgaaaaaatt gcatgcgacg atgcagagta cctgttagtt gcctatggtt
    21961 catcgagccg tatttgccag aaagttgttg atatggcccg cgaaaaggga ataaaagtag
    22021 gattactacg tcccataacc ctattcccct atcctaccaa ggcaattcaa gctatgctgg
    22081 ggcagctcaa aggaatcctt gcagtagaaa tgagtgccgg ccaaatggtt gaggatgtac
    22141 gcctggctgt tgaaggtaag gttaaagttg aacactatgg ccgcatggga ggcatgattc
    22201 ctactccaaa tgatgtttta aaggctttag aagaaaaaat tattaaaggc taaaaaagat
    22261 ggacgtaaaa gatataatca aggaagagaa tttggtttac tccaaaacca agctcctaac
    22321 cgacaatatt atgcactact gcccgggttg tacccatggt gtagttcata aggttctggc
    22381 tgaagttatt gatgagctgg gcatacagga caagaccatt ggtgttgcac cggttgggtg
    22441 ctctgtgctt gcatacaact acattgatat tgactggcat gaggcagccc acggtcgtgc
    22501 acctgcgctt gctaccgcta tcaaccgttt ataccccaac aagtatgttt ttacctacca
    22561 aggcgatggc gacctagcct caattggtac tgctgaaatc atacacgcct gcaaccgtgg
    22621 cgaaaatatt gtggttattt tcatcaataa tggtatttac gggatgactg gaggtcaaat
    22681 ggctccaact tcattattgg acatgcccac ctcaaccact ccatacggac gaaaagccga
    22741 gcttaacggt taccccttaa agattaccga gctaatagcc caactccccg gaacatgcta
    22801 cgttacacgc cagtcgggac acacacccgc tgccgttcgt aaactcaaaa aggccattac
    22861 taaggccttt gagaataccg gaaagaaaaa aggcacatcg ttcattgagg tagtatcgac
    22921 ctgcaactcc ggttggaagc tatcgcccgt taaagcaaac gagtggatgg ttgagcacat
    22981 gttcccatat tacccgcttg gcgatttgaa agacgagtag tagttaatca atttaaaaac
    23041 taaaaaaatg accgaagaaa tcatcatagc cggttttggt gggcaaggtg ttctatcaat
    23101 gggtaaaatc cttgcttact caggcataat gcaggaccag gaagtagcct ggatgccctc
    23161 atatggtccc gaaatgcgtg gcggtactgc aaacgtaact gtaattctta gcgattcacg
    23221 tattagttcc ccaattcttc aggaatttga taccgcaatc attcttaacc agcagtcgct
    23281 cgataagttc gaaaagatgg taaaaccggg tggcacgctg atatatgacc ccaacggtat
    23341 tactcgtcac ccgcagcgta ccgatatcaa catatatagg gttgaggctg ccgaggaagc
    23401 tgctagaatg aagaacgcta agaccttcaa catgattgtt ttgggagctt tccttaaagt
    23461 gaaacccatt gttaagcttg aaaatgtaat aaaagggctt gagaaatcgt tgccccaacg
    23521 tcaccatcac ctaattccca tgaaccagga agcaataagc aggggaatgg agattgtgaa
    23581 aaaaatcaac tagaagatga atgataaaat aaaagcctga cctaaacgtc aggctttttt
    23641 ttattgtctg tatagctccg cggcccgttt tagtattttt tcaacaatat cggatgttat
    23701 gtttttattt tcacccatct tccatcctct tgcagcaaat cggtcaacaa ttggtttagt
    23761 gtcggcaaag ctgattccat gctctggcaa acgtgttttt acgcccaaac ccctaaagaa
    23821 tttctcggtt tcctcaattg cccgctcagg ggttaaattt tttccgttta ggttccaaac
    23881 cctactgcca tactgtagta gcttctcttt tttgaattca accatttcgg ttagtaaagc
    23941 cggataaatt atagcaagag taactccgtg atcaagtcca aagaatgcgg taagctcatg
    24001 acctatcatg tgggtagccc aatcggtagg cacgccagtg gaaatcaatc cattgagagc
    24061 catggtacaa ctccacataa agttggctct taggtcgtaa tcggttggat tttccaaggt
    24121 ttttggacca acctcaatga gcgtttgtag aataccttcg gcaaatcgat cctgaatggg
    24181 cgaattagat gggtatgtaa ggtactgctc aagaacatgg gcaaatgcat caacaacccc
    24241 attggcaact tgtttctgag gaagcgaata tgtagttgta ggatcaagaa ccgagaactt
    24301 tgggtataca gcaggactac caaaagcata cttttccttg gttgcctccc tactaataac
    24361 tgcgccagca ttcatctccg aaccagtcgc agggagtgtt aacactgttc caaagggtac
    24421 agctttctca acctttatgc ctttagtaag aatatcccag ggttcaccct caaaaggaat
    24481 tgatgccaca ataaactttg ttccatcaat aactgaaccg ccgccaacag ccagcacaaa
    24541 atcgacatta ttttcacggg caagcttaac agcttttatg agggtctcgt accgtggatt
    24601 aggctcaatt ccgctaaatt ctaatacttt tttccctttt aaggcgctaa taaccttatc
    24661 gtaaacgcca ttccgtttaa tacttccacc cccataggtt agcagaatcg tttgataagg
    24721 taacaattcc tgtgtaatca tatcaatttg gccttcgcca aatagaattc ttaccggatt
    24781 gaaaaactca aaattcttca tgggcttaac atttttatta aaataaacaa cccatcgttc
    24841 aaaatgttac gatgggttaa acaaattcgt tcggattgat tagattactt gaatagctcg
    24901 ttgtatatcg ataatgatag catcttcctc atctttgtac tcttttttca cctccaattg
    24961 caagttatca tcatcggccc cggcaagcct aacaaaatca agctctggta ttgatgtgct
    25021 caaaaactca aacaaatcct catcggaaag gtattcaaat ccacctattg cattgtatct
    25081 gagcactgta atcaaagcat tctcatctgc atccacaata cttcgtgcta gacgttctga
    25141 gaggtaattc ttaaccttgt tttttgaaac ctgaactttc ttcatggctt tgactttttg
    25201 ccgattaaac tacttttgta ttggtgcatt caattaactt ttcccaatgt tctaaataca
    25261 aatttaattt tttttacgtg tgatttatta aagtggttac tatttacttt actttgtttt
    25321 aatttttaat ttcaggaata tgaacatagc aacaggtgaa atcaagcctc aaattgagga
    25381 gacctggaag aatgttttaa tggatgagtt taaaaaggat tactttatca aacttaaaga
    25441 gtttttggta gaggagaaga aaaaatacac tgtttaccct ccaggttcac agatttttgc
    25501 agcatttaat catactccat tcaatagcgt taaggtggtt atcctaggtc aagatcctta
    25561 ccatggacca gggcaagccc acgggctctg cttttcggtt cctaaaggta ctcctgcccc
    25621 accctcgctt cagaatattt ttaaagagat aaacagcgat ttaggacttc ccataccaaa
    25681 tcacggtaac ttggaaaaat gggcaaaaca gggcgtgctg cttttgaatg caacgctcac
    25741 ggtcagggca aatcaggctg gttcacatca gaacaagggc tgggaaacct ttaccgatgc
    25801 tgccatcaaa gcgctttccg atcaacataa ggggttagtg tttatcctct gggggaacta
    25861 tgctcaggca aaaacgtgta ttatagatac caacaagcat tttattttaa cggctccgca
    25921 cccatcgcca ctttcggcta gcagaggttt ttttgggtgt aaacattttt caaaaacaaa
    25981 taggctcctt acctccatta gtaaagagcc tattgattgg tcgttagatt gattacgaat
    26041 gggttttatt gaaccgttca atttcctgca ggtatttgga acgtatttcc gatattcgtt
    26101 ccaggttggc ctttaaatcg taaattctta ttacatcctg tgggccgtca accttccaga
    26161 aataccctaa ggcagcatca aaaaaactat tctgtaggtt attcttaaca tgactggtaa
    26221 tcagcttaaa gtcgtcccat ttcagttcct ggggaatttc aatgtagtac ttgttatcgc
    26281 cagctaaatc gagataaata ccctcgtcga atcgttcaac aagaaatgtt ttttctattg
    26341 taacaagggc catactattt acaggcttgc tacgtctaaa atcgatgcca tgctcagcaa
    26401 gcttttgttg gattattgga atctcctcaa aacacctgag gtttttaatc cttatacagt
    26461 agtaacgatg atcttccagg tcaatatatc catatgtacc catgcaagca tgtttagcct
    26521 cacgggataa tttttgcata gtccgagcta aaaacatatg gtcgtactcc ctaactagga
    26581 ttaaaaagat tgacctaggt attacatcgg ttggtacatt cttaccgtag tagcccgggt
    26641 atggttcata gttttcaaga accatggtgt tggagtatat tcgctccgaa agcgatttaa
    26701 ccttttcctc cttaaccatt actccacggg tttcaattat cttagcaacg gctgtcatag
    26761 tgttgaattt ttctcaattt acaaaataaa ccgaggttag tcaatagatg caccataaaa
    26821 catttaaata acgtgattct attggcatat cgaggtaatc caggcttatc aaagtctttc
    26881 attccgaggt ttcagaatat cagattatgt tagtcgattc ctcaagtgta aaattcaatt
    26941 gacgaatttg gaatagagta agtcatgtcc agtaaaatcc caagacaaaa aaagaggctt
    27001 atcgcctctt tttgctaaaa cttttcgtaa ttggtgtcgt cgctattatt tgagagcttt
    27061 atatcgatac catcggaaag cttaggtttt gactttgttc ttttggactc cctgaattcg
    27121 gttttaggag taattgaagt ttgagcaatc tttttagtaa cattcttaag gttactcaac
    27181 gttgttaggg tttcttttcc accggtgtca aaaaatgata caatatcaat tagttgctgg
    27241 gcctggctgt tgagttcctc agcacctgaa gccatttcct cgctagcagc agcattctgc
    27301 tgtgtaacct ggttaagctg ctgaatggca gagtttatct gatcggctcc agcattctgc
    27361 tccatacttg ctgcagcaat ctcctgaact agccttgcgg tcttttcaat gtcgggaact
    27421 atctctgaga gcaaccgacc agcatcgtcg gcaattttaa cgccatcgtg ggtaattctg
    27481 ttaatttcct cggcggccaa acggctgcgt tcggcaagct tacgaacttc ggcagccacc
    27541 acggcaaaac ccttgccatg ctcacctgcc ctggctgctt caactgcagc attaagggca
    27601 agtatgttgg tttggaaagc aatatcgcca ataatggtaa ccttttctgc aatctttttc
    27661 atgctattca gggcatatgt agtagaatca tatcccttac ggatgccctc tacgctcttg
    27721 aaggcaatga gctcggcttg cttagcattt tcggtgttat tttgaatatt tgcaaccatt
    27781 tgctccattg aggcagaaac ctcctcagca gaacttgcct gctccgatgc tccctgcgat
    27841 agctgttggc tagtggtact cagttgttcg cttgcagctg caatgtgatt tgaggagata
    27901 tatattgtcg atataacctc cttcagcttc tcgctcatgt cgttaagggc acgtgaaagc
    27961 tttcccagct catcgcctct tttagctgac tcacttgcat gaataaccag gttccctttg
    28021 gataggttat gggctagctc cacacctgag tttacaggtt cagtaatact tcgggtaacc
    28081 ctgtatgcaa ttagtgaact caagatgaca atagacaaaa ccacaaaaat tatcaccgtt
    28141 aatccatgtt tagctgaatc gttcagtaat ccggccgata aaatcactcc cttttggagc
    28201 aaactttggg tatgggttac cgcttcaagg gatttacttt tggcatcaaa aataactttt
    28261 ttggcctcga catgtttaat gaaacttcct ttatactgct caagtgccct aactatctca
    28321 ctaagcccta atctggatgc tagtctggta ttatcatcaa tttccgttag ccatttccta
    28381 tacagttcat cattacctga aatcaggtaa ttcttttcca ttatccgtat attcaaaaac
    28441 tgctgtacgc cacgtgaaac ctctcctccc ataactcggg cggctatggt aagagcagta
    28501 ttcgatgagc tctccagcac tgtagcggtt tgagctataa actgattgct attttcaagt
    28561 attgcgaatt gatcttcaac taccttaata tttccttcaa taattgtaat tagcccatag
    28621 ttttttttag taacaacggg cttaagaatt tcgagttggt ttttaaattt ggtaaagtag
    28681 aacttggcgg agtcgagttc aacctgatta cctgattgaa taaaatcgag caagtgtgaa
    28741 cttaaagcag cgttactagc taataaatta ttcagcataa cctgcttgtt gctctccaac
    28801 cttactttac gattcgaaat gaaaactgca atggcaacaa ctaatattaa accaataatt
    28861 aatgaaaaac ccaatgccaa tcgtttccca attggtaaat tgtttaagtt cataaatagt
    28921 ttgatttaaa gattatactt agctataatt tcatcaactg ttaaggagca attaggcata
    28981 acggattcag gtactggttt gccaaccttc tccgacacaa ggtagcgttt acagcttgta
    29041 tacttctctt taccagctgt gcagtaaatt tttcggtagg tttcgcccgc attcggattg
    29101 aaaaaaacgt ttttcaggta aatggggcac ttttccagtt ttggacagtt tgatattgtt
    29161 tccatacttt ttttttagat atactaaatt taatcaattc ttaatcagaa aaaaaatcat
    29221 ttgtcaaaaa aaatatttta gaaaatcaat ttaaatgtta tctaaataac aacataaata
    29281 ttgctgaaaa tgagttagga acctaattat tgatttgtgg tatatattaa actgatgtac
    29341 catgaaatta caaattcaac agggtaaatt tttaggggaa tgagtcggaa tgaaacggaa
    29401 tgaaacggaa taaatggaat gaatcggaat aggaggaata tgttggagtt agaagaagtt
    29461 agaggaagtt tgagcccgtg aaacgtgagc cgtgaaacgc caagcttgag gcgggaatga
    29521 gttagaatga ataggaatag aaggaatatg aaggagttag atgaaggtag agggaaaggg
    29581 aacagttaaa tcttaccttg gattttcaag cttttacgtt ctaataataa taaggatgta
    29641 gtaagacttt aagaggagat tcgtcaattg aaaaattcaa ttgaaaagtt tggattaaaa
    29701 tgaggtacat tgacttgtgt atcgaaaaat aaaaaagccc cggagtaacc cgaggctttt
    29761 ttgcggtccg gacgggactc gaacccgcga ccccatgcgt gacaggcatg tattctaacc
    29821 agctgaacta ccggaccaat cttttgcttt aacttttccc ttgaaaagcg atgcaaaggt
    29881 aataccttgt ttttaatctt gcaatagtca gctaaaaatt ttttttctga aaaatactga
    29941 aattaacact tccgtaaact cggtgttcca aaaaatgctg attatctgta aagttatact
    30001 tattggaatg ttcaagaata aaccatcctc catcgacaat tactggatga ttaaagatga
    30061 tttgcggtaa ggtatcaatt ccctgcaaat cgtagggggg gtcggcaaaa ataatatcgt
    30121 actgcctgtt acaagactta atatagctaa aagaatctcc cttaatagga tttaatccaa
    30181 taagttttag ctggtttgca gtgttacgga tgaattgcca atgatggaaa tccttttcaa
    30241 ctgaatccac taaggctgct ccgcgtgaaa tgaattcgta gcttatacta cctgtgcccg
    30301 aaaacaaatc aagaaccctt aacccctcaa aatcgaaatg attggcaatt atgttgaaga
    30361 ggctctcctt ggcaaaatcg gtagtaggac gagcacgaaa gttctttgga ggattaataa
    30421 gccgtccacg taaacttccg gatactattc gcatgtaaca cccaaattga atagcctaaa
    30481 gtactctgca cggtaacgta gaagtcggta tgagaaaaat accggtttat gaaaattagc
    30541 ctgatgggta atagagttaa agtatttact tatctcttcg ggcaaccctg ccattaactc
    30601 tccatcacca gtcaggttaa gctgtaagtt atcagtgtta tcaataactg acctagccaa
    30661 tgcattggtg taataaacaa catcggttgt tgatttacac aaatagctat tggccgcaag
    30721 aagctgaccg ttgttgtgaa gaattatggc taaatgctgt tggtcaatat caacctgtaa
    30781 aaagggtttt gcggtgtaac tgtagggaac ctgaataaat gacagtaatg aatgagtaaa
    30841 taaagagcca ccatgtaacg atttccactc gttaataaac tcatggggcg cagcgaatat
    30901 aattactagc gataggcttg gcaattcagt aaaacccaat tcaaagccat cgggtatgga
    30961 atgaacctgc tcaaggagta atttcgattt actaggttca aagaattcat tgggaacaat
    31021 tgtaaagtaa ggtaggttaa atgcaactat aacctttttg aactccccct taagccaggg
    31081 tttagcctca gttagcttac caataaaatt acggaaatca acactaccga tactaaagcc
    31141 ttgccaaccc gtattaaata gtgctatgaa agtgttgcgt atggtatcct tcaccgcata
    31201 cgaaaatcca ttcagggata cctgaatgga tagttcgtat gtagatgtcc gatccctatc
    31261 aaaggtttcg tctatgatgt caatttcttg cattgccaat atactactcc cagttaccgg
    31321 cattattggt ggcctcggtt aaggaaccaa cttttaagcc aggatactta ttaagttttt
    31381 tagcaaggtc gtccatatta ataatttcct gacgatcaag gttctttagg ataaccttgt
    31441 ttggtgcttt acactcaaaa accttaacgc ttacacccga ttgtgtttta agctcacctg
    31501 ctgccagctc aaactgttcg ccaccggtaa acggcacaaa acgtagcgaa tcgtaaggat
    31561 atcccttaag aatggtatcg cgaaccggaa tgagaatggt atcgcggaaa acttttttct
    31621 gaataacagc aagggagtcg tcctccgaac caatctgctt aacaacctta atctgacctt
    31681 ccttcatgaa gttgataagg gtatcgaaac taccggtgta cttaccaaaa cgcgagcggt
    31741 aagcatcctg agcagttcta atacttttta aacgctcaat ggtagcattg tacctacgat
    31801 ttttttcctt ttcaaaacgg attggctcca ttatgctctc atacaggaaa taaccaagta
    31861 tcgcaatgat aactgcaagt gcaatttggt aaactgtttt catttttcgt gttttggttt
    31921 atgtttgcgt gcaaatttaa aaacatctgg ttaaattcta acttaaattt acaaaaaata
    31981 ttgcattgct gtgattaaaa cacatgttat taaggaaata gttgagaggt tgggttttgc
    32041 ccctacagtt ggtcaactgg aagcaattaa caccttatcg gagtttactg ttcagcctac
    32101 cggtaatgat tgtatgttgc ttacaggtta tgccggaacc ggtaaaacat cgctaattgc
    32161 ggcatacgtt aaggctttgg ataagtttgg tataaactac cagctccttg ctcctaccgg
    32221 tagagcagca aaagtattag ctgaatactc cggatgtaat gcttacacaa tccataaaat
    32281 aatttatagg caacaatcca ataccgatgg ttttggacgc tttaccctaa actttaacaa
    32341 atcaaaaaac accatattta ttgttgatga ggcttcgatg atatcgaacc agtcgttcga
    32401 cgatagcgca tttggatcgg gcaagctact tactgacctt attgagtatg tcagaatggg
    32461 caaggagtgc aagctgatgc ttgtgggcga taccgcacag ctacctccgg taggccttga
    32521 tattagcccg gcattgaacc ctgctgagct ggaatcgttt ggtttaaatg ttaggtttgc
    32581 acagctctcc gaggtaatta gacagcagtt cgattcagga attttgcaaa atgctacttg
    32641 gctgagggaa ctggttgact catcaaaaaa tgtattccct aaattcaaca ctgaatatgc
    32701 cgatgtaaaa agaatttcag gaggggaact cctggaatac ctctccgatg agtacagtaa
    32761 aaacgggcgc gaagggaata ttgtaatttg ctactctaac aaacgagcta atagatataa
    32821 tcagggaata cgagcaaaaa ttctattcca cgaggaggaa ctcagcgtgg gtgattacct
    32881 aatggttgcc cggaacaact actactgggt gaacgatagg cccaatattg gctttatagc
    32941 caatggcgaa atagtaaggg ttaagcggat aggtaaaaga actgaacttt atggtttccg
    33001 ttttgctgat gttacatttg agctgcttga ctataataac gaagagtttg atgctaaact
    33061 aatactcgac tctctataca tggatggccc atcgctgggg agcgaaagca tgaaaatgct
    33121 ttaccaggaa gtattggccg attacatgca ccttaaaact aaaaaggctc gcatgaaaca
    33181 aattcaaaat gatccctact ttaacgccat tcaagcaaaa ttcgcatatg cggttacctg
    33241 ccataaagct cagggtggac aatggccgac cgttttcgtt gaccaggggt ttttcaacga
    33301 tgacatgtta actcgcgagt acttgcgctg gctctacact gcttttacaa gggccaccca
    33361 aaaagtttac ctggtaaact ttgacgataa atttttttag cgagttaaaa tttcataaaa
    33421 cagctctaca acattaacat aaccttactc cctttcgttt tgctgttgaa tctttgcagt
    33481 caattttagg gaaagaggta tgaagcgtgt tttttatgtt ttaattgccg tactggcgac
    33541 ttttaatgct gaggttaaga gtcaagaaag tcaaagtatt aaaactcaac taaaattcga
    33601 ttttgtaagc cgccatctat ggcgtggcat gcgaaataat acaacccccg ccgtacagcc
    33661 aactatccga ttcgatggca atatgttttt tggtggcttt tgggcatcgt attccttagg
    33721 ttccgaaaac gttcaggaaa ttgatatata caccggttta aagtacaaga acgttgattt
    33781 tacaattatt gattactaca accctattga tactgctggt tggaagggtg actttttcga
    33841 attcaggaac agtaagaccc gccacaccct cgatgctata atcaccatta acccaacaag
    33901 ccatttcccg ctaagtttaa cctttgcaac catgttttat ggttatgata aggactcatt
    33961 aagcaaaaag aacctttact ccacctacct tgaggcggag tacactttct ttaaaaacga
    34021 caatactcgt atttcatttc atattggtgg aacgccctat aaaagttact acgccagcaa
    34081 gactgcaatt acaaatactg gaataacagt tactaggaat attcacttta actcaaacat
    34141 aaccattccg gttaagggaa gctttattct taacccctac acacaccaag tgtttttact
    34201 agctgcgttt accatactgt aaggataaag gttaaaggtt aaaggttaaa ggttaaagga
    34261 taaaggataa aggttgtcta gtcctgaaat aagtctacag gttagttaat agatttaagc
    34321 gacacactga cccttcccga aaagcatcta ccaagcattg ttatttaatt aacaaatgtt
    34381 taaaacgaac atttaattaa ataaaatttg acttttattt gcatcaaccg tatattgcag
    34441 gaagcacagt gcataatatc aaaaatcaag agtatgagat acaggagctt aaggacagaa
    34501 atgatggtaa acatcctggg tattaccatt gtaataatgg ctgtgacctt aaccgttgtg
    34561 gcattcaaaa gtataaacac agcaaagaaa acggcaaggg aaacaagctc gttgatagca
    34621 tcggatgtta cacggagtgt tgccagctac cttgaaaaac cctttgaaac gctatacaat
    34681 atcaatgtta catttagctc tcttaaattg agcggtgtaa aaaacagaga agtttataag
    34741 gatgtactca aaaaaacgct tgacttaaat aagaattatc ttgccctttg ggcaatgtat
    34801 gaaccaaaca tgcttgatga gaatgactca aaatatgcag gtaaagatgg ttatgatgaa
    34861 gagggtagat ttgatatctc attttacaaa gcaaacaaca ccataaaaag cgaagcgggc
    34921 gaggtttcaa tgtacagcga ggattactac cagataccct tcacatcagg caatgaaact
    34981 gtacttgagc cgtactttta tacatacacc ggcgacgaaa ctggccatag ttactttgaa
    35041 acctcggtgg ctatccctgt aaaagagtcc ggaaaaacaa taggggtaat tggtttggat
    35101 attgaccttt tagaactttc tgagaatatc aagaatcaaa aggtttacca aacaggctat
    35161 atatttattg catcatacga tgggattata gccgcacatc ctaataaaga attaactggc
    35221 gatacgcttt caaatattct gaaaattgaa attgctgatt acaaaaaaat tgttgatagc
    35281 aattcattta gcacggtgac attgggtaag ggaagcaagc aatcgtttgt atttataaac
    35341 cctataaaga ttgggaaaac ccttaagcct tggtcggtag ttgtggttgt gcccgttaat
    35401 gaggtttact cggagagtcg aaaattaata atcacgatcg ttattgttgg cataattgca
    35461 gttgcgcttc ttattatgat aatactatac caagccggca atattactat gccaatattt
    35521 aaagccgtaa gatttgccaa caatatagcc aatggcgatt taacccacaa gctggagcac
    35581 aaccgatccg atgagcttgg aacactccaa tcatcgctcc ttcaaatgca gcaaaagcta
    35641 attgaaatgg ttattgaatt caagagcagt agtggaagta tagccgatgc cagctcacac
    35701 ttaaactcta cagcccagca ggtatcgcaa agtgctgagg agttagcttc atcgtcggaa
    35761 gaactgtcga gcaccatgga ggaaatggtt tcaaatattg agcaaaactc ccaccatgcc
    35821 attgaggttg aaaagatatc gcttgaactg gtaactgatg cccaaaaggt gaaggatgcc
    35881 tccgaaagaa gtatggcatc aattggttca attgccgaaa aaattcaaat tattaacgat
    35941 atagcattcc aaacaaacct gttagcccta aacgccgctg ttgaggctgc acgtgcaggt
    36001 gaacatggaa aagggtttgc agttgtggcc gctgaagtgc gaaggttagc cgaacgtagc
    36061 cgaatggcag ccgacgaaat aaatatacaa tcgggtgaga gcgttcaaat tacagctaaa
    36121 gccacagagt tactgaatca aattattcca aaaattcaga aaacaaccga actgattcag
    36181 gaaattgccc acgccagcaa ggaacagcta acgggctcag agcaggttaa caataccacc
    36241 caacagctta gcggagttac tcaacaaaat gccactattt cagaggaatt agctgctagc
    36301 gcagaggaac ttgctgcaca ggctgagaaa ttgttggaat ttgctgagtc gtttaagatt
    36361 gaaaccgctc atggtgttaa aaaggatgac tctaaacacc aaactatcga aagcgaaaaa
    36421 acaaccccaa agcttgaacc caaaccaaaa acatccgaaa agaaggttat aaaacccctt
    36481 acaaaaaaga aagctgagga gcccaagcaa ccccacaagg ggattgatat taagttaaaa
    36541 ccaagcgatg attctgaata cgagagtttc taacaattga gggctgcaaa tggcagccct
    36601 caattacttt acggtttcat catcccgtac tgcgatagaa tcatatccag attttcaaac
    36661 ttcttccggt aatcgtcaag ggctgcattt atgatttggt gtgcctcatc aactccatat
    36721 acatgggtaa tctcgcaatc gcggtgagtt cccggtaaat ccttgtaggt taggaagtaa
    36781 tgcctcagtc gggtaatgat tgcctcaggg cattcatcaa tattgcggat atgcccataa
    36841 actgcatcat cctttaaaac ggcaattatt ttatcgtcgg cctggttacc gtcaatcatc
    36901 ctcaatcccc caatggggat tgcgtaggct agtaaattcc catgtgcaat gtttttttcg
    36961 gttaacacgc aaatgtcaag gggatcgcca tcgccggcta aatcgttacg ttcggtttta
    37021 tcgcgggcaa tttgtgccac attctcgtcg cagagggttt gtggaataaa accataaagt
    37081 gctggaagca catttgagta cttttgcgga cgatcgatcc ggaggtaacc cgatacctta
    37141 tcaacctcat acttaaccgt atcggttgta accatctcaa tgaaagttgt aactatgctg
    37201 ggggcattag gcccaacgtc aaccccatgc caggggtgcg atttatacct caatcccatt
    37261 agcctgccaa tgggatccat aatcctattt gtcattgctt aaaaaatttt agcccgcaaa
    37321 ggtaataaat gaaacgagat tagaaaacgt tcacctcccg ctcaagctct ataccgaaaa
    37381 gttccttcac ggatttttgg attgaagagg acaattctaa aatttcagta ccagttgcac
    37441 ctccatggtt tacaagcacc aaggcctgct tatgatgcac accgcaccta cctacttgct
    37501 tacccttcca tccggctttt tcaataagcc aaccagcagg aattttatag aaactctctg
    37561 aaacctgaaa accatttaaa tcgggatgtt ccgatttaag cgcattgtat ttatcagcat
    37621 caacaacggg atttttgaaa aaactaccgg cattacctaa ctcagccggg tcgggcaatt
    37681 tacttttccg gatgttgata acggctttgc gtacattttg tactgtaacc tcaccaagct
    37741 tttcaacttc ctcagcaagg ttcccatagt tcaatataaa actcgggttc cttgaaagcc
    37801 gaaaccatac gtgggtaatt attaccttcc cttttagctc ctgcttaaaa atactatcgc
    37861 gatatccaaa acggcactca gaagcattga actcaatgag ttttcgagaa gatacctcga
    37921 tggcttcaac cttaactatg gtatccttta cctcaacccc atatgcacca atgttctgta
    37981 tgggcgatgc gccaattaac ccaggtatat gggataggtt ttcaattcca ccaagattgt
    38041 tctctgcagc aaattcaaca aatttatccc acacaacacc tgcaccaacc ctaaatacgt
    38101 agtattgttc gtcgtcggtt tggagttgaa ctccttgaat agccggatta attactacgc
    38161 cctcaatatt ccttgtaaat aaaatgttac tacctcctcc tagaacaaag attggcaact
    38221 tattatacga agcaaaattt atggcatata gaatcttttc aacggtgttt gccctggcgt
    38281 agtaacttgc ctttacatct attccaaagg tgttgtaagg tttaagcgat atatctttct
    38341 taataacaaa catttggctt ttagtttgcg ataacaaata taactttttt atgtaaaatc
    38401 gtaaattttt atagctttac taatactaaa ccttcaaagc cattcatttg ttattaagta
    38461 aacttcactt atgcgaatcc aaatatttag cacatcatat atagtaaccg accagcggtt
    38521 acaacgcgtt gccaattcac tagcagctta tggatactca gttaaggtat tctgccgcaa
    38581 gcataaaaac ttgagctatg aaaacaataa attcaacgtc aaatacataa accctctctt
    38641 tgaaaagggg cctctttttt atttctttta caacctaaga atttttttac gaatactttt
    38701 ttcgcgctct gacattattt actcaaacga tcttgatacc ttgccgggat gcgcattagg
    38761 ctcaatagta aggtgtaaaa agttgattta tgatagtcac gaacttttta ccgaagtgcc
    38821 agagctcatt ggaagaccta tcacaaaatt cctctggcat attcaggaaa aaatctttgt
    38881 taaacgtgca aaggcagtaa ttacagtttc agagggtgta gctaatgagc taaagaaaag
    38941 gtacaacctg aaaaacattg aggtaatcag gaatgttccc atacgaactt ccacggaagt
    39001 tttcaaagat aaacaaccaa caattatata tcaaggtgcc ctgaatatgg gaagaggtat
    39061 tgaactggcc attgaaatga tgaaacagtt accttgctac aagctgctca ttgttggtaa
    39121 aggagatatt gaaataaaac tgcgccggca gatgcttgaa agtgatcttt ctggcagggt
    39181 tcagttttta gggcaactaa caccagaaaa tttaaggctt gttaccccta ctgcatggtt
    39241 aggtctatcg ctcgaggagg atatgggact gaactacagg tacgctttgc ccaataaact
    39301 atttgattat ttagctgcac atgtgccagt gcttgtaagt gatttacctg aaatgaaaag
    39361 ggtagttgag gaatatggga ttggcattgt tgctcaaagc cggaaaccaa tagaccttgc
    39421 caaccagatt gccgactttt ttgaggataa ggagggctat gaaaatactc tgaaaaatgt
    39481 aaagaaagcg gcagaaaaac tatgctggca gaacgaggaa cccaggttgc tcaacctgat
    39541 taactcgtta aaaaagggtt agtacttgtt gaacgatact atttcgttaa agtccttacg
    39601 ccgtttctgg cgaacttcgt cacttgcagg gtatccaagg gtaataatta ggggaaccct
    39661 acgcgactta ggaatatcca gcaacttttt cacagcgggc tcatcaaacc aacccagcat
    39721 gcagctacca agtccctctg ccttggcagc aagacaaata tgctcagcag caattccaat
    39781 atcaatgaga gggaatgttt tgccctttac taaactcccg aagttagaat ttagattagc
    39841 gggttccatc acaataacga tgtgaacggg agcctgtttg gtaaagtggt tgatgcccaa
    39901 tactctgctt gcagcagcat cggcaagttt attttttagt tctggttcat caactacaat
    39961 aaaatgccag ggttgggcat tacatgccga tggtgccaaa cgggctgcct caataattcg
    40021 aagaattact tccctaccaa caggacgtgt atcgtaagcc ctatcgctct gccggttttg
    40081 aacaaggttg taaaagtaat cgctatggtt tgtaaacatc tgctatttgc tttaaaactt
    40141 cggactctac agttactttg cccttggcag ttaaagcaag ctgaaccatg gctttagcca
    40201 catcgcctgc atcaatgcct cggtatcgcc ttaatgggcc tacaaatata aacctaaacg
    40261 ctcgaaataa ccaaatccct atactctcac ctagtctata ttcattccgc ttaccaagta
    40321 ggattgatgg acgaacaata gcaaccagct caaaatcgaa ctttttgagt tcctcctcca
    40381 tttgtccctt tgtccgcagg taaaagttac gtgaactttg atttgcacct atggatgaaa
    40441 tagcaacaat tcgtttaact cctgcctggg aagctttgtg aacaatttca ataaccatat
    40501 cgagatctac agctttaaat gcctctttgc tcccagcctt cttcatggta gttcccaagc
    40561 atatgaaaac atcataagta tcaggctcaa ttgagatatt cgctccaaac tcaacaaatc
    40621 tgaccttttg gaatggattt gattggttta aattccttcg gtaggcaact atctcatgat
    40681 aatttctatt ctcatcgagc acacggagca ggttataacc aactagacca gtgcttccaa
    40741 atacagcggc ttttcgttcc ataacagttt tttaaaaagt aacaaacaaa ccactaaaag
    40801 gttattctgt attggggtat aaccattatt ccaagctgat agacccactg cgattcgcct
    40861 gtgcgtgtat taaacctatc gccatagata ttcttattgt taaacaaatt tgtaacctca
    40921 attgcccatt cctgggtaaa tcctaacttg ttcaacttgt acctaatagt caagtcggtt
    40981 ttaaaataat cgcgaagctg taattcataa gccttacttg tatcccattc cctggaatac
    41041 ctgttctcag tagggttgta aacagctgac caaggagtgg ctcgctttcc accactgtat
    41101 gacaccttgg catcaaaact aaaagtcttc tttgactttt tgctattgga cagattaaac
    41161 tccttaccaa atagagcatt tgtaatgtag tggtggttaa atgcagtgct gtgggtaata
    41221 ccatcgctac cctgatactg tgaattaaaa agtgatgcag ttaataggta atagtagccc
    41281 ttgtttagga aacgctccaa tgttaactca acaccatagt tttgtccatt gccaccgttc
    41341 ttaacgtagt ccggtaactc cagaacaaaa cttgcaccct ggtttaacag cgagtaagaa
    41401 ttagattcag ctgcatctac agcagcatca aaaatatact ggtagtaaac ctccgcttta
    41461 agccgggtat gcgaggctag taaccaatcg taacccgcaa caaaatggtg gctacgaaca
    41521 aagcccaaat ccttgtttgg ctggatataa gagtcgtcgc taagtcgtac acgcccaagc
    41581 acaatggata acgacacaac ctgactatgt aacccatacc ctagattaat tgaactattg
    41641 gaagatattg cccacttaat ggcaatgcga ggctcaacag accattccct gttcaggaaa
    41701 aagtaaatgg catgtaaacc ggtattgaac gtaagcgtat tggttggacg atactgccaa
    41761 ttaatataag gctgtgttaa caatgcatca ccctcatcat tccttagaat aaggaatgcg
    41821 ttatcgttct ttttaaaaac gctatcataa tagttaaatg ctagcagctt ggctattaca
    41881 cctattttta tttggctttt agttgagaac cgtttattaa atgacaggtt tcctgatatt
    41941 gtgctctcaa taaagttatt gcggtagtat ggaaattttt gtaataaatc ggttgaaagc
    42001 gagtccatta ccgttttaaa ctggtgagcg ctaacagcaa ccgaaagttt tgtgtaagta
    42061 ctctgattta ccatgaatga atggtttaac cccacaacac ccatactgga accattagta
    42121 aggtcaaacc cttcgccacc ataaaaatcg attttatctt taagggtatc cctacggcta
    42181 tcccaaattt caatatcgct taatccgcct aatccaaaca aggagatatt tccaagctta
    42241 gttcgaggta aatttatttt aaaagatatg tcctggtaat aaggcacacc cactgttccc
    42301 agatccattc caagcttatc aaaaacacct agggttgagt acctgtaatt aacaatgaac
    42361 gaggaaccat tacttttagc aatcggcccc tcagctccaa actcaaaacc attaaagcct
    42421 atttgaccca aaaactcata tttttcgttg tttccgctac gcattttcaa gtcaaatgca
    42481 cccgagatgg cattaccata ctctgccgaa aatgcccctg aaataaagtc ggagttttcc
    42541 aaaaggttac tgttaagcat acaaacaggc ccacctgtgg ttccggttgc tccccaatga
    42601 tttgggttgg gaatatcaac accctctaag cgccaaagta aacccattgg gctatttcca
    42661 cgaataataa tatcgttccg ggaatcgtca acacccataa cacctgcata gtttgcagcc
    42721 attctggcaa catcgtttcg actaccagca taacgctcgg tttcctcaac agtaaaggtt
    42781 cttgcgctta ctgtggcaag tttattcatt ggagttgatt tatcggactt tgcagttaca
    42841 accaccgcct caacattaac agccaactcc tccatctcga tatttaaaac caactcctta
    42901 ccaacctgca ggttaaggtt tgatagcaca acatcattat accctaaaaa agaaactttt
    42961 aggctcacac gaccaatttc aacagattcg aacttaaagc gtccttgctc atcggtcata
    43021 actcctctta aaggatcaga atctagtaga attacatttg caccaggcaa tgtcattttg
    43081 gtgttcttat ctattacaac acctctaact gtttgtgtta agatattagt tgagaaagcc
    43141 tcgaaaccaa ttagcacaaa agtaattaaa aataactttt tcatctttta tttagtatta
    43201 acattacgat gcaaagttga tatttggaat accccattac aaataagata ggatgaactg
    43261 ggataaaacg gggatgaaac agaaagggtt gacacaaatg tcaaccccta atcttttatc
    43321 aatatcgatt tttagtaccc tgccgcttgc ccatcctttc gggattcaga ggctccaata
    43381 taaaccccat ttttttcatc gaacataatt gcctggtatc ccccgtaacc atccaagcta
    43441 aacccaattc tgtgtccctt ggccaataat tttctaattt cctggtaatc gaacccggat
    43501 tcaaggtaaa ccattcctcc atcggtcatt cgttcgccag tgggctctga actgccctcg
    43561 tgctgtattc gtggggcgtc tcctgcttcc tgtaaattca ttccaaagtc tattaaattt
    43621 acaataatct gggcatgccc ttggggttgc attccgccac ccatcacgcc aaaactaacc
    43681 catggtttac catccttggt aatgaagcca ggtataatgg tatgaaacgg ccttttgccg
    43741 ggagcataaa catttgggtg tccctcctcc aaactgaaaa gttctcccct atcctgaagt
    43801 acaaaaccta aaccagttgg tgtcattccg gaacccattc cacggtaatt actctgaatg
    43861 agcgaaacca tatttccctc cctatcggca acagtcaaat aaactgtatt gcttgtttgt
    43921 aacatgcccg catcgtaggt tctggcagca cggttcaaat cgattagctt tgccctttcc
    43981 cttgcatact cttttgaaat caatatttca taagggattt taacaaaatc agggtcagca
    44041 tagtatcgag ccctatcctc aaaggcaagt tttttagcct caatgaacag atgcatgtat
    44101 tctgcgctgc caaaccccat cgattttata tcgaactgtt ccagtatgtt tagaatttgt
    44161 agagtagcta taccctggcc attgggaggt aattcccaca catcgtaacc cctatacgac
    44221 gatgacacag gctcaaccca ctccgattta tgagttgcca aatcctcgta actcagaaaa
    44281 ccaccatttc gtttcatgta ttcatcaatg gcttttgcta ttgagccttt gtaaaactca
    44341 tctcgtccct ttagtgcaat cagttcataa gtatttgcta ggtaagggtt tcgaaatatt
    44401 tcacctttgg aaggggcttt gccgttgggc ataaaaacat cagcaaaacc ctcgtattgt
    44461 tttaagcgca ctgcattgcg ctgccagtag tatgcaataa gttcggaaac cggaaaaccc
    44521 tcgcgggcgt atgaaatggc aggagataga attttttgca taggcaaccg gccaaacctc
    44581 ttatgtagct caaaccagcc atccacacag ccgggaaccg ataccggaag cggtcctaaa
    44641 gctggaatgt gagcgattcc attcttttta aagtattcaa gggttagcga cattggtgaa
    44701 cggccactgg cattaaggcc ataaagtttt tttgtttttg catcccatac aatggcaaat
    44761 aaatcgccac caattccact gccagtaggt tccaccaatc ctagcatagc attggctgca
    44821 atggcagcat caacagcagt gcccccctgc ttcagaatat caatggcaac ctgtgttgcc
    44881 aagggctggc ttgtacaagc cataccatta cgggctataa cctcggaacg tgttgcaaag
    44941 tttaacccag tcattctatc ctgagaaaac agggttaagg ttatacccgt gagcaataaa
    45001 attgagctaa gaattttttt tcccatatca aaatgattta aaaaaggcca accgtttttt
    45061 ttcgattggc ctttaaatat tagtaaccga atatttgctc caatgttacc ttagttacag
    45121 gcccataaag tttttccagt tcctggaaat tcatgtcggc ctctttgcct aaaataatgt
    45181 aagtttttgg tttccctttt acgaattgtt cctggaattt cttaacatcg tcaaaggtat
    45241 aagtaggtat ttgtgaatac agtttcttac gaatatcttc gttccaaccc atttgtttgg
    45301 cattcaggta gctgtaaagt actcgcatgt ttcttatccg gttgtttcgg atatcggaaa
    45361 tcaaagcctc cttggaaaga ttgaagccta ttgtattttc cggcatagcg ttgaacagtt
    45421 cgttaaaggc gttgaaggca tcaactacct tgtcgttttg agttgcaatg taactcatgt
    45481 ttatgaagta atcgtcaggt cttgagggaa tggtgtacct tgagcgggcc gtgtaggcta
    45541 ggccacgctt ttcgcgcatt tcttggaaaa caatggcatt catgcctcca ccaaaatact
    45601 cgttgtacaa agtaataatg ggataaaggt tatcgtcgta cttttgaccc ctgcttaaag
    45661 tttgtaggta cgattgcttt gcatcgtaat gggcaaaaag cactctatcg gaggttgttt
    45721 caaggggttc aaatctttta ttttccggtg ttggaatgaa ttttttgggt aaacggtgct
    45781 ccttgccaag cataaccttt aaatctttcg atgctaatgg gccataataa agcactgtat
    45841 ggcggtagga acttaaactc ttaattacac caattaggtc gttggctttt agctcattaa
    45901 gttctttttc gctcagtata aatttttggg gattgttatc tccataggta gcgtagctaa
    45961 ctaatgcact aaatactgcc gattggttac gtttagcatc gttacgggct tttatctggt
    46021 tggcaatata agcctttagg gctgactcgt cgggttgaca atcgttaagt agtgattcca
    46081 ttaggtttaa ggccttggcg gcattttcgc ttaaaccaga aagagtaatg taagtatctt
    46141 catcgttaac attaatgttg aaggtgcatg ccaaacgata aaactcctgg ctaacctcct
    46201 ccgcactcaa ctttgaggtt cccaggagct gtatgtaatc agctgcatgg gtcagtgccg
    46261 gattacttaa actaccaaac ggatagtagt aaaccaggtt aaatgtccca ttctcagtgt
    46321 tctgtttgta aaggacttcg atatttttat taatcttaat tttggtaaaa tccttattat
    46381 agtcaataaa cactggttca acaggtgtta ccttagtttc ctttactttc tttagaaaat
    46441 cggattcaac atcgcggtta atatgaattg gagtaattgg aggcttagca acctttgcca
    46501 catcctgggg tttaccctga cgcttataaa ccacaacata gttattccct aggtaatcgt
    46561 tggcaaattt tacaacatca tccttgctaa ttttttgtaa ccgttcaatg ctattaacaa
    46621 cattttccca ttcaatacca ttaaggaaag cattcatcat caaccgggca cggctcgatg
    46681 ctgattcaag agcgttcatc ctgttcaact tatagttgtt gattgctgcc tcaagcatcc
    46741 agtcaggaaa ttctcccttt tttaataact caacctgctt taatagtaga tcctttacct
    46801 cttccagtga ctgtcccttc ttatttcggc caccaagctg tagaattgaa taatcagtca
    46861 tcactcttgc tgatgcataa gccctaagtg caagctgttt tttattgata tttaaatcga
    46921 taaggcccgc tttagtgtta taaagaatgg agttaataag cgatagcata tcggcatcgg
    46981 ccgaggttgc gccggcaaaa cggaatccaa tggatacgtt ctcagcctcc aagccaacaa
    47041 cctcatattc cttgggttga ataattggct cctcaggaac aatactaaac tcaggcaagg
    47101 gttttgcctt aagggttcca aagtacttat ccactacctt tattgcctcg tcggggttaa
    47161 aatcgccggc aaggataact gccatgttat tgggaacata atacttgtca aagaactcac
    47221 ggatattttt cattgaggga ttcttgaggt gctcagcctc accaagggta gtttgctgcc
    47281 cataggggtg attggggaat agaccacgca acagagcctc accaacttta cgggcatcgt
    47341 tggttaacga catgtttttt tcctcgtaaa cagtttcaag ttcagtgtgg aaaagcctga
    47401 gcaccggatg gctgaaacgg ttagcctgaa tcattgccca attttcaagc tgattgcttg
    47461 ggatattctc aatataaatg gtgtagtcgt ttgatgttgc agcatttgtc ccccttgagc
    47521 caatggcact cataagcttg tcgtactcat ttggaatggc aagctttgat gcttcaaagg
    47581 agatactatc aattttatgg taaatcgcag cacgcttgag cgaatcggtc tcgttacggt
    47641 aaacctcaaa caattgctca atggtattga tcatctcttt ctctttttcc cagttgagcg
    47701 taccaaaatc gggagttccc ttgaacatca tatgctcaaa gtaatgagct aaacctgtgg
    47761 tttccttggg atcgttctta ctccccactc taacggcaac gaatgtttgg atgcggggct
    47821 catccttggt tactgacatt aacaccttta acccgttatc aagggtataa attcttacac
    47881 ccattgggtc gtttggaaca gtttcgtact tatactgctg tgcaagggcg ttagcagtaa
    47941 aaaccgtaac tgcaaagagc agtaactgaa tttttttcag tatttgtttc ataacttttg
    48001 ttgatttagg ttttgatttt gactaataat aaaattcaaa gcttaacacc cctgtttttt
    48061 aaacttagaa attgtatttt tatcaaaaaa gatatatgtt tatcaaaaaa atttaagtcc
    48121 catgaaacgt aagctaaaga taaggcagaa aattttactc tacatcctaa cggtaactgc
    48181 aattctttac gttgcctccg taggttacat cctttggcag tcgagggcaa atatttatag
    48241 cgatgccatt gaaaaaaccc aactaaccgc aaaagtatcg gcaaaagagg taggagcaat
    48301 atttgaaaag gagttatcgc ttgtcagaac cctttctcag gcctttacag tgtataaaaa
    48361 tatgccacat gaacagtgga aaaagctttt tcttgacatg tacatccctg ttttaaggga
    48421 aaaccctgaa atttactcaa tatgggatag ctgggagtac aaatcgtttg tgccgggtta
    48481 cgataaggat tacggaaggt ttgttatcac cacttggcgt gaaggaaacg agataaaatg
    48541 gctatacgat gagcgtagca taaatggcga tcctcctatt tatggtaaat acaaaaagct
    48601 ggggcaggaa gcaatttggg aaccctacgt tgaccaggtt actaagggaa aagccgatat
    48661 taaactgatg attacctact actcccccat ttacatcaac aacgttttca gcgggatagt
    48721 tgccagtgat gttagcctgg aaagtctcca ggagatggta tcgaaaatta agccggtaaa
    48781 aggaagcttt gcttacctaa tttcaaacga aggaataatt gccgcacatc ccagcaagga
    48841 ctttatcaat aaaaaaattg atgaagcatt caaaattgat aacgaaaaag aaaagattgc
    48901 agaaagaata gccaagggcg aagaattcta ctacacaaaa accgacgaaa caggcaaaca
    48961 actacttatt tgccattcac caataattgc tggcaaaaca aaaactccat ggtcattagc
    49021 cctatcggta cctgttgatg taataatggc gaatgccaag gaaacccttt acgtttcgct
    49081 atttgttagc attgcaggct tacttgtaat ggtgctggta ttgctttttg tgtccgacaa
    49141 cctaactaaa cccataaagc gaattaccga tatcctgaaa cgaatggcca ggggcgaagt
    49201 atcggatgag ctaaaggttg acctcaatac tggtgatgaa attgaagaga tggcgttagc
    49261 gcttaacacc acaattgatg gtattggtca taaaacaacc tttgccaacg agataggtaa
    49321 aggaaactac acaacctacc tagagctact cagcgataag gatgtgcttg gaaaatcgtt
    49381 aatacacatg cgcgacagcc ttaaaaaggc agctgaggat gaagagaagc gtaaggttga
    49441 cgatcaaaaa cgatcctggg caaatgaggg ttacgcaaaa tttggtgaga tactccgcca
    49501 aaataacaac gacctgcaaa aactttgcga tagcgtaatt gccgcattgg taaagtatgt
    49561 tggagctaac caggggggaa ttttcctttg gaacgatgag gacaaaacca accaatactt
    49621 tgagctggtt tcagcttttg catgggatag gaaaaagtat ttaaccaaac aaattgagaa
    49681 aggcgaaggg cttgttggcg cctgcgccat ggaaaaggaa accatcttca tgacagatgt
    49741 acctgacgat tacgttaaca tcacatcagg tttgggcgac gcaaatcccc gatgcgtgat
    49801 tcttgtaccc cttaaacatg aagatgaagt tctaggcgtg attgaaatgg cctcgttcaa
    49861 ggtgctagaa aagcacgaaa ttgaatttat tgaaaagatt gcagaaagca ttgcctcaac
    49921 catcctttcg gttaaaataa atgcacgtac taagatgtta ctggagcaat cgcaaatgca
    49981 agccgaagag atgaaagctc aggaagagga aatgcgccag aatatggaag agctacaggc
    50041 cacccaggag gaagttgaac gcaaatcggc tgaaattgag gagtttatca aatcaattca
    50101 aagctctgca tacgttattg agtacgacct taacggcaat attctgaatg taaacgattc
    50161 ctttatccag tttgttgggg ttccccgtga acagctaatt ggtagccacc atagcgataa
    50221 ccttgagctt tccgatgtac aaaagaagga gtatggaaca ttctgggata accttaaagc
    50281 cggtattagc aaaaaggtta aatcgacaat tacctggaaa ggcaaaacat accacttcct
    50341 tgaaacctat atccctgtaa aggatagcga aggaagggtt tacaagatta tgaaacttgc
    50401 ctttgaggat ggtgagttca agtagaaaaa aaagcggcca caaaggccgc ttttttttat
    50461 aagcctatta gcttcataat tggatcgtcg ccaccaaaca acagcttttc agggttctca
    50521 atcatccgct tgatatccat caggaatgct gctgaatcct taccatcaat aattctatgg
    50581 tcatacgata gggcaacata cataataggc ctaatttcca ctttgccgtt tacggctacc
    50641 ggcctatcaa caatgttatg catgcccagt attgccgatt gtgggtagtt aagaatgggc
    50701 gtggaaagca ttgacccaaa aataccacca ttggtaatgg taaatgttcc tccattaagc
    50761 tcatcgaggg taattcgccc ttcccttgcc ttggtggcaa gcctgagaat ttctttttca
    50821 atttcagcaa aactcatggt ttgtgcatta cgaagcacag gaaccattag ccccttaggg
    50881 gtttgaactg caatccccat gtcaatgtaa cttggggtta ctatatcttc gccatcaatc
    50941 atagagttgg tcatggggtg taacctcagc gcttcaactg cagcccgcac aaagaacgac
    51001 atcaacccta acttaacacc aaacttttgc tgaaaatcgg tttggtatct tgctctaatc
    51061 tcctgcacac ggctcatatc aacctcgtta aaggttgtaa gcattgccgt ggtgttttta
    51121 gcggttacca annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    51181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntatcaacct
    51241 cgttaaaggt tgtaagcatt gccgtggtgt ttttagcggt taccaaacgc tcgctaacct
    51301 ttttcctaag ctgcgacatt ctaatgcgct cctcggaacg taccaaggtt ggtgcttcat
    51361 tgcgcttgtc agaggtaact acaaggttat ttaatgtatc cttaaccatt tgtacatcac
    51421 cagccgttat cctcctgagg ccattaagaa catcatcaat attcatatta tactcatgca
    51481 tgagttctcg tgccagcgga gtaaccttta ctttatcagt aatcttatct tcatgtgtag
    51541 gaaaatctgt ttttccctcg gttgttactt taggcttttc aactaattct acttttggtt
    51601 ggctcttact gttggcagtg ggcatggctt tgggagattt ccctgcaaag gatgtatcta
    51661 ttctgcagca aacctcattg acctttatca tgccagctga aacaactagg gcgatttttc
    51721 ctgcttctgg agcaatgatt ggtagtgttg ctttctccga ttcaacttcg gctatttcct
    51781 gatctttttc aacaatatca ccatcggata caagccagcg cgtgagttca acctccgata
    51841 tcgactcgcc gggcgaggga attcttacat ctacgatcat aaatgcaata ttttatagtt
    51901 aactactttc tttcgaaata ttcgtactga ttgagcctat cgaatcgacg tgacccttct
    51961 tcgcactgca atccgcaata cttgttcttt aactcgcagg tgcagggtct aaatacctta
    52021 gtgattatct ccttttgctc caggttatga atcttactta aaccaacagc aggacttccg
    52081 cttggcgccc ttgagataac ctcaagaggt acaccagtta tcctatcctt tacaaagttc
    52141 catgccccca tattttgggg ctcttcctga acccagagcc atttctttgc gtttggatat
    52201 ctatcgagta ttttttcaac ttgacgagat gggaaaggat atagctgctc aatccgaacc
    52261 aatgcaatat cttttacatc aagttcttcc ttacgctgca gtagatcata gtaaattttt
    52321 ccactgcaga atacaactcg cgatacagct ttttcgtcaa cattactgtc gtcaatcact
    52381 tctaggaaac tgccactctc gagttcctga agggttgaaa tatttttagg gtgacgaagt
    52441 atacttttgg gtgtgaatac aaccaatggg gttctgaaat cgcgctttag ctgcctccgt
    52501 aatacatgga aaaagttggc aggtgttgta gcatttacaa tttgcatgtt gttgcgcgca
    52561 gaaagggtaa gaaaccgctc tattcgagcg ctggaatgct caggcccttg cccttcaaaa
    52621 ccatggggta aaagaagcac aagccctgat tgcaaccccc acttatcttc ggctgaacta
    52681 atatactgat caataataac ctgagcaaca ttatgaaaat cgccgaactg tgcctcccat
    52741 atggttaacc cattaggtaa agccacagaa tagccatact caaaacctaa aaccccatac
    52801 tccgagagga gactattgta aactgaaaac tttgccctag tatttggaat attttgaagg
    52861 gggtaatact tttcctcgct tccctgaatt gagtacactg agtgacgatg cgaaaaagtt
    52921 cccctctcag aatcttgccc acttaagcgc accgggtgcc cctcggcaat aagcgtcccg
    52981 taagctaaaa gttcagccat tgcccaatca accttttgct gggtatagtt ctcccgacgc
    53041 tcatcaatta tcttaactaa ctttttgaaa aaatcaattc cagagggtaa ctcaagtaaa
    53101 gcgttggaga ccatattaag catttccttg cttacacctg tatcgggcga tttttcgaaa
    53161 tcattactgg atgagtacct ataactactt gatttttcag gaataaacct accaatcctg
    53221 atttttggat ttgcctgcgc taacacaaaa cgttcctcga gcatggtatc gtaattcttc
    53281 ctgatggtag cggcatcctc aaccgatatc accttagttt caaccaactt ggaagtataa
    53341 atatcccgca cattgggatg ctttgcaatt aaatcgtaaa gtacaggctg ggtaaagcgg
    53401 ggctcatcgc cctcattgtg cccatacttg cggtagccca gcaaatcaat aaaaacatcg
    53461 cggtgaaagg tttgacgaaa ctcaacagct aaacgaatta cgtgcaataa tgcctcaggg
    53521 tcatcggcat tgacgtggaa aacgggcgat agggttacct ttgccacatc ggttgagtag
    53581 gtactgctac gggcatcaat atagtttgta gtaaagccaa cctggttatt gaggacaata
    53641 tgtatagttc caccagtgga gtaaccactt agccgcgaca tctgaatggt ttcgtatacc
    53701 acaccctggg cagcaacagc cgcatcgcca tgaatgatta tgggaatcaa actattaaca
    53761 tcgccctggt aggcatcgtc aattcgggca cgagcaatac cttcggcaac cgggccaaca
    53821 gtttcaaggt gcgaagggtt tggaacaagg tgtaacctta ccatttttcc cttatcggtt
    53881 tcaatggtat tgctgtagcc taagtggtac ttaacatcgc caagggttga ttcatcgtcg
    53941 tatgcctcac caataaactc ggcaaaaatt tcctcgtagg gtttacgcat tatgttggtt
    54001 atcacgttta aacgtcctct gtgagccata ccaatcacaa attcctttac cccatgctca
    54061 gcaccatgtt caatagtagt atcaagtgca ggtataagcg cctcaagccc ttccagcgag
    54121 aatcgctttt gtccaacaaa cttcctatgt ataaactgct caaatcctac agctgcagtt
    54181 aggtggtaaa gaatatgctt ctgttggtcg gggctaaact gggtatggtt ttgggttccc
    54241 tctaccctac tctgtatcca atcaaccagt tttggttccc gcaagtacat gtattcaaca
    54301 ccaatactac ggcagtatat cctgtttagc ctttcaatga tttcagaaag agtagcgtta
    54361 ggcaaaccta taagttcacc agctttaaat cggttgtcta aatcttttgt acctaatcca
    54421 aagttttcga tatcaagggt tggagagtag ctacgacggg tccgtaccgg gttggtcttt
    54481 gtgaacaggt gtccccgcat acggtatgct tcaattagct ttatcacctt gaattctgag
    54541 tcagcaatac aatcctgtgc gctgtaagag ccagaaatag ccaggtcgaa acccaaaaaa
    54601 aacttttgcc agccctcatc tactgaattt ggattactct tccacatccg gtaaagttca
    54661 tcaatagtct taggatcgat attaccaaga atgttttcca tacttaaaaa ttttcaataa
    54721 ccaacactaa gcagatacta aagttcaaaa taaacctcaa ttttgagtaa aaagatttgt
    54781 gcaaagtcaa ccggtgtaac caaacagctt tttctgcttt atagttttaa ccacatactt
    54841 gggcaccatg ctttcccatc gcttatcgtt gctttgtatg aggcttaaaa cctcatccga
    54901 ggttatttta agccactgct catttacgcc tgttaggcta acaatcaaat cgttctgcat
    54961 caggtagtgg aaaatgtgca tcacatcatc gggaactgta atatcggtga tgtacctaat
    55021 attgccagtg tttcggtcaa gtgcaggata aatatagaac tttgtacggg caggaaacat
    55081 tttgcctaca gcctcaagta agcctccggg taaatttgca tagtaggatt tatcaagtac
    55141 cttcataaaa gttggtatac ccataactat tctaaggttt ttatgcttaa accttccaaa
    55201 gtaatcaacc aatctataaa actccctaaa gtttgatatt aaaacgtttt gccccatccc
    55261 atttagcaaa tcaacccgat ccaggaaatc gcgttctttc agctcgccct cttcaagaag
    55321 attattaagt gtgatttcac agatctgaag ggtattatcg ggtttaaaca atgcattttt
    55381 cttaaaatga gcataagatg tctttaagat atcgaagccc acgtaggtaa ttggtctgaa
    55441 gcgtccccta agtaccaatg cgttcttctt atacagcata tcgtcgggct cctgaacatt
    55501 gccataccga tcgaaaaaaa tcgccggagt cataccattc ttaaccagtt gaacccccaa
    55561 gagcctattg tccacgtaat ccagatcagg cccgcccatt cgaaccatgg ttatctcaac
    55621 cctatcggtt gaaagattat ccataagcga ctgtaggaat agtgtggggc ttttatggtt
    55681 gtaaaaacag gcataaataa ggtttacgcc aagagttcct aaggtatact gctgcaaaat
    55741 agcatcgttc tcattcaggc gaacgtgaat aaccacctcg ttagccgtgc ctagtttggt
    55801 aagctcaaaa cgtacaccaa gccaaccatg tccctggtta tcctttcgga agttaagcgt
    55861 ttcaaccgtg tttgcaaaaa caaagaaacg tttatcggga cttctttcag agttaagcac
    55921 atcaattagc tgtttgtact cagcatcaag catttgctgt aagcgtttta ggcaaacgta
    55981 ccgtccgcta tcgccatcgc tatacaggta atcgctaaac accttatcgt atgcggagat
    56041 ggttttagca atagttcccg atgccccacc tgcctggaag aatgcacgtg ctacctcctg
    56101 tcctccgcca atttcagcaa tcgtaccata aattgttgga tcaaggttta tggctaaagc
    56161 cttacgcttg gtggttagta tatcgcgttc catgtgatgg ttttgtataa aattacaaaa
    56221 aaaagggtag tttgttcaaa gagttaacca actaattagt ataggcaaaa tattgtttat
    56281 tattcaagtt actacaaatt ccaagtatta agaaaattaa tactaatttt tagttagtca
    56341 attgcatgga taatattttt atgagttata tctcagatga tactcaaaat ggattataac
    56401 atttcgatta tctcaaattt tagatttaat atttaaagga aacgctaaac ataaaataac
    56461 gaggaattaa attatagctt actttactat agtaatattc cgagatacta cttacaacgt
    56521 agttattatc atttaaaata ttaaaagttg agaaagacat tgaccatttt gtatttggga
    56581 agttatatgt aagttttgag ttaattagaa aataactgtt gttactgtac gttagaacgg
    56641 tactttggtt actatataat tctgatagta aatctaaact taaattctga gaaggtttta
    56701 tatacaaatt acaaaatatc ctttcaatgt tattttgctt ggttttctta tttactattc
    56761 gtccttgtgt aattgaataa cctgaatgaa aatttaaaaa tgaattgaac gatgaacgta
    56821 tttcaaaagc tagagtagtt gaattatttc tgttcctgtt tgaaatcccg aacaagaaat
    56881 tatcataatc aaagtaaagg cttgttaatg aaaattttat gtttgaacgt ataagtttaa
    56941 taaaataatt tgcctcaata ttacctatta ttgtatttct attcttagta attgtatctt
    57001 tagaaactac atagtttatg ttaggttcaa tattttgagt aataaatttg gattcataaa
    57061 tatacatcaa actgctattc ataaaaaaac gtgtatttaa tttaccgatt aaatagctgg
    57121 ctcctaaatt atgcatattc ataagagatg gaaatgtacc acgatttatt gaattgtaac
    57181 tattaaaata gtaatgatta ataatactag tatagggaaa aggtttaata tctgaattat
    57241 aaaataaact taatgtattt attctattta ataaccattt gaaagtaata tttggtaata
    57301 atatgattct cttataatga taattatttt gtttttcata aattttattt aaacaaattt
    57361 tataacctaa acttatattc aaactatcag atccccaaat tagttttgat tgtaaaaatg
    57421 cttcattact gttaatattt gtattcccta aaaaagatgg atcgggtgta ttgttattca
    57481 aagaatcaat tatgacataa gagctcttta aatcataact aataatattt gctccaactt
    57541 ccccttcaat cagtaacata ttggatatcc ggtgtttaat accagtaact cccaaaaaat
    57601 aacagtaatc attgtcaagt ttttgtttcc ctcttccatc aatggaacca attgtggata
    57661 gatgagttat aggtattggg ttaatcatta attcctcttt taaattgcca ttcaatgtgt
    57721 atccaattaa ataagctatt gttctattat ttattttttg gctaaaatta aggtgattga
    57781 ttagcttaaa agaactacct gtcccttcct caccaacagc agtttgattg aaatctgtta
    57841 gtgtatttgc atccttgtaa gtgcttgaaa aaagaaaatt atattgaatg aatgatttat
    57901 tactggggct actttcaatc tttgatttga taaatgcatt tttattgttc atgtaaaaat
    57961 tgtaattctc agtatttatt atgcttagtg tatcataact atagtttttt aatgtttgcc
    58021 ttatgaactt atcgtttata ttactataca ccgttgtaac agttaatttt gttttttttg
    58081 ataggttaac tgcctcattc atagacccta aaattaaatc aatatcattt accctttgac
    58141 gcttgagaat aggtcggtag gcatcttggg ttaaaataaa atctggagaa tctgccttag
    58201 tcaacttatc gtccgaagtt tcagacaatg ttttgaatag ttctgatgat ccaacagttg
    58261 ggttatcgcc aatgctattt gaatttccaa ttatcaaact gttaaccact ctattaaaat
    58321 tcattaaact cacatttata tcgtgcttgt taatagtatt ataacccaaa tcaaaatttg
    58381 tataaatttt tttatatgaa ttttcagtta gttttaggtt tagggcaact ttatcggaat
    58441 ttgaaatctc ttttaatatt gggttttcgg aatagttact aatagcctct accttttcaa
    58501 ttgcatttgc atgtaccctt tgtgttatca ttctatatcc cttaccaaaa aaatcggttt
    58561 gctcaaccat tactttctca acttctttcc ctttgaaagt aacccttccg ttatcatcaa
    58621 catttatacc cggaagtttc ttgagaacat cttcaaccac cctttctgta ccatttgtaa
    58681 acgattttac attataaact atggtatcag ttttaatagt tattggctta tcgccgtgtt
    58741 ttataacttc acttatttca taagcctgtt cttcaagagt tacatcaatt cttatagggt
    58801 ttactaatgc tgaatctaat gggattttta taaatttaac aatcttgtta aaccccaaag
    58861 ccaaatactc tatcctaaaa tcgccttgtt tcgaagtagt aattttatag tagccacttg
    58921 tgtttacaaa tgcatacgaa agtatacctg aacctgaagt tgagtataaa actatttgag
    58981 catgatcaat cggctttcct ttaaaatcac ttacataacc ttcaataata gtttgagcac
    59041 tgactttaga aaacaaaaac actgcaaaaa ggaatagcaa ttttttcatt tttataaatc
    59101 taaattgttc aattaaagct ttaatggatg tgtatatgtg aagtcaataa ttcaagttta
    59161 gatgctaaaa ctcatattcc aactccaaac ctacattatg gactttaaaa gagcttacat
    59221 taaagccaat atcctcaggc tgcctagctt gtataacctg aatattcgat tctaaacgtt
    59281 tactttcatt ctgaactctt tccctttggg atgttatata ttccctcaaa gtgtatactg
    59341 gatactcttt tttgtaagct atgttgcagg aggtaacatt accaatattt atttcaacag
    59401 ctgaaaagat cacttcattc ttaatatcag ctacctgaac aattaatcct ggtaagccat
    59461 gtagtttcca tggcccataa gttattggta tttctggtgt gaaccaggca atataatctc
    59521 tacccctgaa tttacatgtg gctttttggc attggaaact gcctatcgtt tttgtttcgg
    59581 attctataat ccactctatt cttcctactt cctcctccaa atagaaataa ggcacagttt
    59641 gttttttctt atttgcaaaa taggtttcat tcacaagctg atatatttta ctgttttcct
    59701 taaccgtaaa aatgttaaaa ggtatgcttt tggactcttt caaagtattg ggatctcggc
    59761 gtcttaccac tgagaattga cctggggcag tctcaaccat cgaaaggttt tcatttctac
    59821 tatcatttcc ctttccattg ctctcaaccg cagtaaaatc tgaaatagcc ataaatgaag
    59881 agccatactg gttaaaacaa agcactcctt ttgcctgaat aggataagta gtggtccata
    59941 cgctttcgta aaccacacta cccgaaagtc tctctgtttg agaaatcgca atattgctta
    60001 tgaataatac agaaccaaca aatgcaaata ctctgtttct catcttttta gtaatattaa
    60061 ttagacatct actggaaatt taataaataa atattaaaga gggaatactg caaattgcag
    60121 tattccctta aagttagcat aaaatttgat cgtatgcttc tgcaacatct aatgcagcca
    60181 tagcagctct tctataaaaa tcagaggtac atttccccca aacaaaaact gagtaatgat
    60241 acactgtgcc attaccacaa tcggttgaga aagtaaatgt tgcatagcac gttttatcct
    60301 caggatttga attttgagaa ttgtccattt gatccgataa atcaacattt aaatttatac
    60361 taactgtaac aacagccttt gctgaaggct ccatggcacc aatcttattt tctgcaaaca
    60421 gagtgccata ggttagggtt aaacaaaaca acaaaatcaa tcttttcata gtcatttggt
    60481 ttagtttgat ctactctctt taggcttttc gattccgcct gctatccttt tgttggccaa
    60541 ctatttgaac agcagaacaa aaaaaaaaaa aaaaaaatga aaatgtcaag actttttgtt
    60601 tatttataat gcatttatta tcaataaaac ctaatttatc atacatctat ttaattataa
    60661 atatcaattt tactaatacc cattcataga ttcattgtcg tttagttagc aaaaatttac
    60721 atatctgtca tactgagcgg cctgtcccgc cctagcgggg tgcgaaggat ctctaatcaa
    60781 cccaataaga tgtttcgctt agctcaacta gacaatagct tcacgaacaa cgattcacga
    60841 aaaacgaaca acgcttccag ccctgtaggg gctaagcatt tgtaattcca tggctaaccc
    60901 taaatgtata gcgatgcaca ccattcatat gccaaagaac atgtgtcaaa cgttgcatcg
    60961 caacagaaaa gcaaggcaaa gagcggttta caaaaaaaca acaaggatta agggtaaaga
    61021 gatgcttcgg cgaagtctat tcctataaat cgggactcag aacgacagcg tgatgtttcg
    61081 catgcgcgca acatgacaaa taacatttat tcattgattt attaaaatgc aaaaacgagg
    61141 ttggattata tccaaacctc gttatacaat atacaaagaa ttaatatacc tctactcagg
    61201 ttttatcaat tcaatgcttc gcttcacaaa ccggcttaag tcctcaccct taagcatgtt
    61261 gttagccagt aaagctaaat cgataagctg ctttaccagc ttgtaatttt taccaaagtc
    61321 ctttaggact gtgctacgtt cctcctccag cttatcgagc tgcttttcgg catcggcaag
    61381 ggcatcctta tcagcttggg gtatctcatc gtccttttta cccttctgag ctttttccag
    61441 ctcctcaacc ttagccttaa caggctttat tttttcgtta acctcgttga gcctttcgct
    61501 aagctgcttg cttttagcct caataatgga tttaaccaag gggtgcgaca cgttaaccac
    61561 aaggttacgg ctatcgggca tgctaccata aaagcttgaa gttgggctca tggccgacat
    61621 atccttcatg cggcgcatga actcggactg ggtgataacc accggatttg ccccctcgcc
    61681 aagttcctca aacgataccc agtagtggtc ctgagcgggt agcgcaccct caaaaactaa
    61741 ccttaaatcg gcttgttcgt cggcaggtaa gctaacctcc tttcggtcct ccttaacaat
    61801 gagcttgtca atcacatcgg aatcgacacg ggcaaatcgg gtatccttca gcttggcctc
    61861 caaagtgttt atgaaatgag gatcaagttg tccgtccatc agcagcacat cataaccacg
    61921 cgcttttgcc tcctcaatgt aactgtgctg atcgtccttg tcgctggcat acaggtaaac
    61981 tagggttttg ttcttatcgg tctggttttc cttaaccagc ttttcgtact cctcaaaggt
    62041 aaagtattta ccatcaacat ttttgagtaa agcgaacttc tcggcacgtt catagaactt
    62101 ttcgtcggta atcatgccaa atgtgatgaa cagcttaagg tcgtcccatt tcttctcaaa
    62161 ctgttcacgg tcattcttga atatttcctg aagcctatcg gcaacttttt tggtaatatg
    62221 cgatgatatt ttctttacat tcgaatcgct ttgcaggtag ctacgggaaa cgttcaaggg
    62281 aatatcgggc gagtcgataa cgccatgaag caatgtcaaa aattcaggca caataccttc
    62341 aaccgaatcg gttacgtaaa cctgattaca gtacagctga attttattct tttgaatgtc
    62401 gaagttagtt tttattcttg ggaagtaaag aatacctgtt agattgaaag ggtaatccac
    62461 attcaggtga atgtaaaaaa ggggctcctc ctgggcggga tataaatcgc ggtaaaactt
    62521 ttggtagtcc tcatccttca actcggcggg tttgcgagtc cagagcggat tggtttcgtt
    62581 aattattcta tccttcccag tatcaacctc cttgccatcc ttccattcct tttcggttcc
    62641 aaaggctatg ggaatgggca ggaaccggca atacttgcga agcaattcct caatcctgtg
    62701 atcttcaagg aattcctttg aatcgtcgct tatatgtaaa acaatatcgg ttccgcgagc
    62761 ttcacggtca gcgtcttcaa ttttatactc aggtgtaccg tcgcaactcc agcgaactgg
    62821 tttggcgcca tcctgccatg aacgggtaat aatctccacc ttatcgctaa ccatgaacga
    62881 tgagtagaat ccaagcccga aatggccaat aataccgtta gcctgagctt tatacttttc
    62941 caaaaactcc tcggcacctg aaaaagcaat ttgattgata taacggtcaa tttcatcggc
    63001 ggtcatgccc acaccactat cgctaattgt gagtgttccc tttttcctat caaccttcac
    63061 cctgatggta aggtcgccaa gctctccctt aaaatcgcca accgatgcta aagtttttag
    63121 cttttgggtg gcatcaacgg cgttggaaat taactccctt aaaaatatct catggtcaga
    63181 atacaaaaac ttttttatta ttgggaaaat gttctcagtg gttacgccaa tttttcctgt
    63241 ttgcattgta catcaatatt ttaggttaaa cattatcgat acatacctta cccaactttt
    63301 caattccaat gccaacaagg gcgttgataa agatatcaaa atgaattttt gagtatcaac
    63361 ctgatagtca atataatgac aaagcgcaat ggagttgaca agaaaatgtc aattagacaa
    63421 gcgtatgcaa ttttgtcaga ttaaatagta accaataagt agatatgacg ccatgataat
    63481 gattgttcca atccagatat acaacgctat tttaaaatct ttcttacgtt gtgccagggc
    63541 ttgtttgctg aatagcaatg cgatatggtt gtttattcgg agtagtgcgc tatcgtcctt
    63601 attgataaca gccacagcac caagtttttt aacgtaacca ccatactcct gttcgtcctc
    63661 attagcaagc ataatagcat ttacaaaggg tttaatcttt ttgagcgatt caagaatctc
    63721 tataccattc atcagggttt gattcttttc atcgtaaaat gtatagccaa taagggcaac
    63781 atgagttatg cgcttgctaa gcttctcggt ttttaaatag ttaataaact cctcgccatt
    63841 tgcaaatttt ttgatataaa aaggctcaga gcattccaag ccgttaaaaa ggatgtcgga
    63901 ccactcaatg ctgtagtcaa caatgtagat tgctattttc cttcttattt tatcaagggt
    63961 atccattcct gccatgtgtt gaccacagtt attttggtta tatatactaa atcgatgcgg
    64021 taaaagtatt attcatttca atatgagtaa aggctaaact acattgtaat acaaatttat
    64081 aaaattttaa accgaagtgc catgaattaa caaattcaac cgggctaatt ttagaggaat
    64141 gagtcggaat agacagaata tgatggagtt agaggaagtt agaggaagtt tgagtccgtt
    64201 aaacgtgaat cgtgagccgt gaaacgcaag atttgaggcg gaatgaatcg gaatgagtca
    64261 gaatgagtcg gaatagatag aatattatgg agttagaaga agttagaaga agttagaaga
    64321 agtttgagga agttatagga agttagagga agttaaagga agttaaagga agttaaagga
    64381 agttaaagga agttaaatga agtcagagga agttagagga atagtaaaaa gtgaacggct
    64441 aaaccaaatt gccatgaatt aacaaataca accgggctaa taaaaatgtg aatacagaaa
    64501 catttttaga ggaatttagt caatagaaac acccctaaaa aacaataaat aacttgtttc
    64561 tattgactta tctaaggaaa ttccaaactt aaatttgata aaaaaagccg ggattacccg
    64621 gctttaaaaa ttaaagttat gttagttata caggggtaga tatgaatatc tcttggaata
    64681 ttctaacatc tgctcaacat agctatcggg gtattccacc aaggcatcta ccacactgtc
    64741 gccctgagta accgggataa tccgggggtt aataaaacca ccatagggtg caatgttaag
    64801 ctttgcaaac ctatcgagaa cctgcttatg taaatcgaaa tcaaccttaa cggcgtagtt
    64861 ctcaaccaaa gctttaccgg cctcaaaatc gccagttgat ttaattcgct ggatttcttt
    64921 aagaagctgc ccaaaaagtt gacggagctt ctcaaaatcg ttaaccacaa agtaaacctt
    64981 gccatcgcgc tcttttcgct caattacatt atcggctttt ccctgctcat agcaccactt
    65041 tgaaattaac tgacgattac gcatatgggc ctgttctatg ttcttaccca gctcaacacg
    65101 ggtaagctga gtcataagac catttcgtat ataattagaa tactgaacct tagccacatc
    65161 cagattggga attaggccaa gttcaaccat tttggggtcc attatgtagt aaagagcaaa
    65221 cagatcggca cgggcctcct ccaacggcga accgtaattt ttaagctcat cgcctttaac
    65281 tccatcggct agctggcctg atccgtggcc aaggcactca tgcaaatcgg tatgtaggct
    65341 gcttgccagg tatccatact ttttgtttag ctcaacctcc tgtgccgacc aggcaaactc
    65401 ctccaggaaa ccatttccca gagccgcctg atcgtaggcg tatgtaatgt tatccatggt
    65461 aaccgatttt gaaccatgaa ctttacggat ccaatcggca ttgggcaaat taatacctat
    65521 aggggttgaa gggtaacaat cgcctcccag ctgagcaacg gtaattacct tagccgttac
    65581 tccctttacc tctttcttct tgaactgatc atcaattggc gagtgatcct caaaccattg
    65641 agcgtttccg ctaattgttt gagtgcggcg ggttgcctca tcgtttctga aatttacaac
    65701 cgactcccag cttgccttgt aacctaaggg atcgccgtag ttctcaataa atccattcac
    65761 aaaatcaacc ctggattgta aatcgttaac ccaaagtaca ttgtactcat caaacttttt
    65821 aaggtcgccg gttttgtagt aatctatcag tttctctatc acctggcgct ggtggtcgtt
    65881 ttcagcaacc gtagcggctt tctccagcca gtaaacaatt cgttcaattg ctttaccata
    65941 cataccgcct aaatgccaaa ccttttcaac aatctttccg ttctccttta ccaactttga
    66001 atttagcccg taagatatag gggttgaatc ggcagggttt gccaagctgg cataaaactt
    66061 ttcggcttca gcttgggtaa ctccctcata gtaattacag gcagactgag ccaccatatc
    66121 aacaccagct tcctggttaa cccttttggg agcaacatta gggtcaaaaa tgattggcaa
    66181 atacttttcg atgatttgaa ccttacctcc caactccgaa ggaaagagtg aatcaggggt
    66241 ttgatttaca agctgtgtaa agtacccagc gctaaaacca ggcataattt tatcgttcga
    66301 gtagtggtga tggattccgt ttgagaacca aacccgtttc aaataaacca taaactggtt
    66361 ccactgctca ccattccgtt cgccaggata ccctttatat aatgcctcca gtattgagcg
    66421 cacggctaga ttgtgcttat aattctgatc ccaaataata tcgcgcccac acttggcagc
    66481 ctcgcttaag tagtaaacca acacgcgctg ttggggtgtg agtttttcaa actcaggaat
    66541 ttggtaacgc attatgcgta tgtcggcaaa ctgatcgatt tgccacttga aatcatcgtt
    66601 cttttttcct gagtcgcaac tggaaattgc aaatccggca gttgcaaaac aaattaaagt
    66661 cattagcctt ttcatttatt taacggttag tttgattgtt tttagctatt atactaaaga
    66721 taattagcaa aaaagtaaac cataatcatt ttttgggtga tgagtcaggc ttgtaaagga
    66781 atcccggctc tttaatgtca accggagcgt ttaccggctt aggttttgat cgacgtggat
    66841 ttcgtaaatc caaatctctt acatacaccc attcagtacc ctcaagctta aaaccatcga
    66901 atgagccatc ggggccgtaa tactgaaagt cgcctgcaaa ttcagggctt gaaggtgaaa
    66961 gatgatcgaa aacaatggtt ttgctatcat cgttgtatcg taacaccatg tttactcggg
    67021 cattgaattc aaatacaatc cgcgatagcc tatacttacc atcaacattg aaaaccggag
    67081 agccaaaaat tggagtacct tggctatcga aagttagaac atcaataatc ttttttgatg
    67141 taaaaagatt attgaaatcg tatcccaaca taacgtaagc atttccgtta gacgttggga
    67201 actgagccaa ttgatagtaa agcgcggcca tccatcgttc agcaggtagc ttttcgtttt
    67261 ttacatcggt aatacttttt cgatcatcct taagctcaat caaagccaca tttccatcgg
    67321 cctttttaac ctgcaataac ccaaaatatt tttggtaacc acccggctgt gggatattcc
    67381 aggtaaaaat acgaaatctg ccatcgggcg attttaattt ccctattagg attgaatcaa
    67441 aaggataagt atacgagctt tgaacctcca aggtactcct gagcatacct aaaaatattg
    67501 tattcaacga atcgcgacga gagtaggagg tttctgctga aatactgtta aaaactaact
    67561 ttaatgaatc ctcagtttta acaaaatcga atgctggctg agaaaaaagt gaaagcggca
    67621 aaaaaaacaa tcccaaaaaa actacactgc gcataacaga aactttattg aatagcattt
    67681 aaaccctcaa ctatcagatt tgcaacgatt cggcattcat cctctgtaat tattagaggt
    67741 ggagcaatgc gaactgctgt attacagaac aaaaaccagt ccaaaatgaa tccatggtcg
    67801 aggccatact taatcaacgc ctgaaccctg tcaaacgttc ccagctcaac agccatcata
    67861 agtccagctc gacgcacctc cctaaccttt ggatgctttg ccaaagcctc ggcaaggatt
    67921 gagcctttat gttcaacctg ttcaacaact ttctcatgca tcaaaaagtt gagcgatgca
    67981 atggctgcag cgcacgaaac cggatgcccc ccaaaagtag ttatatgtcc gagaattggg
    68041 ttgtgggtta ataccctcat aatttctttt gatgaaataa aggcccccaa tggcattcca
    68101 ccaccaaagg cttttgccag taacagaatg tcagggataa cctcaaatct ttgaaaagca
    68161 aacatgcttc cagtacgccc cattccagtc tgtatctcat caaatataag taatgcaccc
    68221 gtttcactgc atcgttgttt caacttacga aggtaatcaa cgttaggtaa tcgaatacct
    68281 ccttcgccct gtatgggctc aatgatcacg caggctgttc gggttgttat atgatctaaa
    68341 tcggctatac tattaaagtt taataaacgt acatcgggga gtaaggggcg gtagtttcgc
    68401 ttctgctcct cgcaacccat tacgcttaaa gcgccatgtg tgcttccatg gtatgcattt
    68461 ttgaatgcta caatctcact cctaccggta aatcgttttg ctagcttaag cgcaccctca
    68521 acggcctcgc taccagagtt aacaaggtaa accgaattta aattctcagg cagtaacctg
    68581 gtaagctgag cggtgagctc aacctgaggt ttttggatgt actccccgta aaccataagg
    68641 tgcatgaaat cggctgcttg actctgaaca gctttaacca ccatggggtt tccatgacca
    68701 aggttactta ctgagatacc ggaaattaaa tcgaaatact ttttaccatc tggcccaaat
    68761 agaaaaaccc cgtcagcttt atttatctca agcattaggg gcgattccga tgtttgggct
    68821 aaatgctcca gaaagagctg cctcaacaca ttcatttcgt cctacttatt tgcaattaga
    68881 tttagctcgt ttatcaatga ctcgcgatac gatttcccaa tgggaatggt tttacgacct
    68941 tcgttgctcc gtatcgatat tacgttatcc tcaatggttt caattttacc aacattaact
    69001 ataaaggagc gatgaacacg cttaaacctc acgctgggta gctgattttc aatagatttc
    69061 atggttaaat gaatggtaaa ccgttcatgg ctggtcacaa taatcacgta gttctccata
    69121 gcctcaatcc aaaggatatc gccataccta acccttacaa gcgagttatt tttcttgata
    69181 aaaatttccc tatcatcgtc gaatggcgaa cgtaactggt tgtaacgctc ataggatcgg
    69241 gatactgctt taaaaaatcg agggaaagta atgggtttta aaaggtaatc ggttacactg
    69301 tagtcaaatg cctttagggc atacttttcc tttgacgata taataacaac ttggggttta
    69361 gctgaactgg tctcgagaaa atcgattccc gacatctcgg gcatttcgat atcaagaaaa
    69421 accaaatgaa catcgtcgtt ttggttttga aggaaactaa gtgcctcaat aggattggag
    69481 aaagagcgaa ccagattgat aaagtcaacc cttgaagcat aatcctcaat taaccttctc
    69541 gacatctcat cgtcatctac aagtatacag ttcataacca attgttttgc tgtgattttt
    69601 ttacgaattt acaaaaattt gatagaaaaa attcaggcta atgcatttta agttcattta
    69661 aaaaacgttt ttctatcaaa cttgagctac ggattgaagc ccttagccac tgaagcatca
    69721 gctcatcctg ttcatcagac gataacctat aatcaatttc agaattccga atccggtttg
    69781 ttagctcata aagggttatg gcaactgaaa ctgaaatatt gtagctctca gtgaagccaa
    69841 acatgggtat tctaacatac tcatcggcat tctttagcgc taaatccgat aaaccggtaa
    69901 gctcagtacc aaaaagcaaa gcgacctttc cctttgataa atcgagttca ctaatggtac
    69961 aatcattatc gtgcggagta gtagcaacaa ttctataccc cttttctctt agggcgttaa
    70021 tcgcctcaag ggtgttattc tcaagtttgt tgtaccggtg tagagtaagc catttggaag
    70081 cccccatatc aacctcgcta tcaactgaga atttgttgct attttcaatt atgtgtatgt
    70141 cctgaacgcc taaacaatcg caagttctaa gggtagcgct tgcattctga gcctggtaga
    70201 tatcttcaag aacaacggtg atgtagcgtg ttctattact aatgatgctc ctgaatagct
    70261 gataccttct atcgtaaata aattgcgaca ggtaatcaat cagcctttta cgcagttctt
    70321 tatcggtggg gtcaatattc agcttcattg aacaaaaata aaaaaggaga accaatgcag
    70381 gctctccttt ttgaaaaaaa ggcaaataat tagttaactt ttccgctaag ttcgctacca
    70441 gctttgaact taacaacctt tttagcctta atgttgatgg gctttccggt ttgagggttg
    70501 cggccagttc tagcatttct cttaacaacg gagaaagagc caaaacctac tagagcaacg
    70561 cggtcacctt tcttaagagc ggtaccggta gtcttgatga aagcgtcaag tgctttctta
    70621 gcatcagcct tggtaatttt ggcttcagca gcaattgcat cgattaattg tgctttgttc
    70681 ataacaaata gaatttaggg ttaataatag ttagttgact atcaaagaca aaattattac
    70741 atttattaat aaatgcaagt tttttgaaat ttttttttgc cccgatagcc atttttgttg
    70801 atttttaaaa aacattctct ttatatgatg ctaatttata gcgttttttt gatacaatgc
    70861 aagtaaaagt atactttttt caacattttt tcaacaagct atttgtcaaa aaggaaaaag
    70921 caactatttt tgcagcggag aaatggcaga gtggtcgaat gcggcggtct tgaaaaccgt
    70981 tgtccgttta cgcggaccgg gggttcgaat ccctctttct ccgccaaaaa aaacgaaggg
    71041 taacaccctt cgttttttat tttgatacca acaaccaccc cataagcaca cctatcccca
    71101 tactactcca cggcgatgag gttattgggc aactaccact gttacaccct atataataat
    71161 agtacaggta acccgctact attccaatgg caacggcaag aactggatag gtaacccttt
    71221 tcttttcctt gaaccattgg taaaagtgat ccactttggt ctttaaattc atattcatct
    71281 atatatatat ttatgtttat tgcaaaagta acaacatttg caaaaacttg ttctatatgt
    71341 ttgcatatag atataaagta attatttttg caagcccaaa cggtttaatg gagagatgcc
    71401 ggagcggtcg aacggggcgg tctcgaaaac cgttgacccg ggtaaccggg tccgagggtt
    71461 cgaatccctc tctctccgcc acaaagtagg aatgttgtgt tcctactttt ttttatttat
    71521 accgggccga agggttcgaa ccgagaacgg ctctgccgtt cgagctcacg agcattgcga
    71581 gtaatccctc tctctccgcc acaaagtagg aatgttgtgt tcctactttt ttttatttat
    71641 accgggccga agggttcgaa ccgagaacgg ctttgccgtt cgagctcacg agcattgcga
    71701 gtaatccctc tctctccgct agaaagtagg aatgttgtgt tcctactttt ttttattcat
    71761 accgggccga agggttcgaa ccgagaacgg ctttgccgtt cgagctcacg agcnnnnnnn
    71821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    71881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnataccgg gccgaagggt tcgaaccgag
    71941 aacggctttg ccgttcgagc tcacgagcat tgcgagtaat ccctctctct ccgccacaaa
    72001 gtaggaatgt tgttttccta ctttttttta ttcataccgt gccgaagggt tcgaaccgag
    72061 aacggctttg ccgaatattt caattcttta ctctcaaaaa atcaacataa aaaaagccgc
    72121 tcctgaatag aacggcaaaa aaactaatat ctaacacctt tacagcaact gcatactgtg
    72181 aagccttgca tagtaaccgt taagtttaag cagctcgtcg tgttttccgc gctcaacaat
    72241 ttcgccttca tgaaggacac aaattaagtc agccttgcgg atagtagaaa gcctgtgtgc
    72301 aataacaatg gaggtacggt gtttcatgag gttatcaatg gcatcctgaa ccaacctttc
    72361 cgactcggtg tcaagagcac tggttgcctc atcgagtatc atgataggtg gatttttcag
    72421 aattgctctg gctatgctaa cccgttggcg ctgtccaccg gaaagtttac ttcccctatc
    72481 gcctacattg gtatggtatc cattgggggt ggcaataata aaatcgtgag catttgcaat
    72541 ttttgccgca tttaccacat cctccatttt ggctccatca actccgaacg ctatattatt
    72601 ataaaaggta tcgttgaaca gaattggatc ctggttaaca ttacccatca ggttcctcaa
    72661 actggttatg ttgtaatcct tgatatttac accatctata aaaatttcac cctcagtaac
    72721 atcccagaaa cggggcaaaa ggtcaaccat ggtactttta cccgaccccg attgccctac
    72781 aagggcaaca gtcatacctt tcttgatggt aaggttaatg tttttaagga cccaatcgtc
    72841 ctggtactta aagcttacat tgcggaattc aatatcatga ttgaacgatt caatgatttt
    72901 agcaccagcg ctaaccttga ttggatttac ggcattaagt atggtatcga ccctatccgc
    72961 tgaagcaagt cccttaagta cgttgtagta tgcatttgag aacgacttgg cagggttaat
    73021 aatcatataa aatattccaa tgtaagcaat taatgactcg ggccccaaag tgcccttgcc
    73081 tgcaaatata agattgccgc cataccacag aacaagaaca accacaannn nnnnnnnnnn
    73141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    73201 nnnnnnnnnn nnnnnnnnnn nnnnnnngtt ctgatgggag ccaaatagta taatgaaagg
    73261 tatgtaaaga ggtttttaag gaatgaggct actaaaacca atacaataac aaatagtaac
    73321 gccgatgctc tgtcgtgtgt tttaattatt tcacttaagt agtagttaag gtattgagca
    73381 aggccctttg cggaaatcac gaactctggt tttacctcaa caagaggaac agttccaaaa
    73441 agaagctgta aaaagggtat taacatggcc aatgagaaca gcgaaaagac agctgctagc
    73501 acattggtaa aaacattaat cacaacatac catttgaatg gataaaggta tttgagtatt
    73561 cggataaatt tcttcatagt atgtgtttat aataattgca aaagtaacct atttattcag
    73621 tgaaattaat ttgaatagcc tttattgtct ataattaaaa ctatttaata ccaaggtaag
    73681 tgaaaggagt cagctctaat agttcccttt taaccgaatc ttttacattc aggctattaa
    73741 caaagtttgt aaaggtgtca gatgtaacct gctgatttgt tcgggtaagc agttttaaag
    73801 catcgtaagg gtttgggtaa ccctcgcggc gaagaatagt ttgaattccc tcagcaatta
    73861 ccatccagtt actttgtagg tctctattaa ttgcatcggt gttaacaatt agcttgtcaa
    73921 gtccattaag tattgatttt aatgcgataa aggaatgtgc aatgggaaca ccaatgttcc
    73981 tgataacggt tgaatcggtt aaatcgcgct gaaggcgcga gattggaagt ttgttggcca
    74041 tgtggtcaaa taaagcattc gccaatccta aattgccttc ggcattctca aagtctatcg
    74101 gatttacctt atggggcatg gccgatgagc ccacctcgcc ttccttaatt ttttgcttga
    74161 agtattccat ggagatgtac atccaaacat cgcggctaaa gtcaataaga atgctgttga
    74221 tacgccgcaa gcaatcgaac atggcggcaa ggttatcgta atgttctatt tgggttgtag
    74281 tttggctccg gtgcaatccc aacttgtagt tcacaaattg atttgcaaat cctacccagt
    74341 caatacttgg ataggctgca atatgtgcat tgaaatttcc agttgctccc ccaaattttg
    74401 ctgagtatgg tatgtgcttg agctgctcta gctgaacctc gatgcgttcc acaaaaaccc
    74461 gaaactcttt gcccaatcga gttggcgatg ctggttgacc atgagtatgg gctagcatgg
    74521 gaatatcggc ccactgagta ctcagctctt taagctttgc caataacttt tcaagcagtg
    74581 ggagatattc agtcttaagg gaatcgcgga gcattgcagg gaatgcagtg ttatttacat
    74641 cctgcgatgt taaaccaaag tgtataaatt ccttatacga ctctaaacca taggcatcaa
    74701 acctctctcg cagaaagtat tctactgcct ttacatcgtg gttggtaacc ttttctattt
    74761 ccttaaccct aagcgcatcg gattcaccaa agcttttgta gatttcgcgt agtatggtga
    74821 attttgattt atcaaacccc tggagttggg gcaaaggaat ttcacaaagg gcaataaaat
    74881 attcaagttc aacccaaacc ctatacttaa ttagggcaaa ctcggaaaag taacccgaca
    74941 gcgattctgt tttttttgca taacggccat cgattggaga aatggaatgc aaactatggt
    75001 tcaacatgct ttacaaaatt tgcccaaatt tactaaacat aactcaatgt taaaggttta
    75061 tgtaagcagg ttttttagca atgaacaata acttttcaaa attgctacct tagcaaacta
    75121 atatttagcc atgaaaaagt acaaggtatc ggccatatcg tatttaaata cagcgccttt
    75181 tgcttatgga ttaaagcatt caccggtatt caacaaaatt gaacttttat acgattaccc
    75241 ggcagaatgc gcccgaaaac ttgccaacaa tcaggttgat ttgagtttac tgcctgttgg
    75301 tgcattgagc gattttagtt cgtttgatat tgtaagcaat tattgcattg gctcaattgg
    75361 aaaagtacgt tcagtggcac ttatgagtaa tagtaattta agcgatatta aaagaatata
    75421 cct
//
LOCUS       DAOSAL010000004        68285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_313_length_68285_cov_49.806244, whole genome shotgun sequence.
ACCESSION   DAOSAL010000004 DAOSAL010000000
VERSION     DAOSAL010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 68285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 68285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68285
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_313_length_68285_cov_49.806244"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="PK049_01140"
     CDS             <1..723
                     /locus_tag="PK049_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60189.1"
                     /translation="ILLRHDVDLKPQNSLATAIIERKLGIQGTYYFRVVPESYNEDII
                     KQIADLGHEIGYHYENMDSVITRFRGKGIKFKNAEEERESIIDAAYEEFCENLERFRK
                     IYPVKTICMHGSPRSKWDNKEIWKKYDYRKLGIIGEPYIDIDFNEVFYLTDTGRRWDG
                     FRYSVRDRVPQQQKWIEQGLVFRTTNDIIKAVNENRFPSKAMITIHPQRWTDNYLEWT
                     KELVTQRAKNIVKWGLMRLRDR"
     gene            complement(954..2099)
                     /gene="wecB"
                     /locus_tag="PK049_01145"
     CDS             complement(954..2099)
                     /gene="wecB"
                     /locus_tag="PK049_01145"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOK60190.1"
                     /translation="MKKLKVLTVVGTRPEIIRLSRVMAKLDQSPAIEHITVHTGQNYD
                     YELNQIFYDDMGIRPPDHYLDAAGDSATATIGQILIKIDPLLETLKPDAMLVLGDTNS
                     CLCAIPAKKRHIPIFHMEAGNRCFDQRVPEETNRKIVDHIADINLTYSDIAREYLLRE
                     GLPADRVIKTGSPMFEVLNHYLPRIQQSDVLSRLNLKRGEYFVISAHREENINSERNF
                     TNLIHSLNLIANTYGYPIIVSTHPRTRKMIEAKGVQVNPLVNFLKPLGFTDYNALQLN
                     AYAVLSDSGTISEESSILNFRALNIREAHERPEAMEEASVMMVGLNPDRIIQGLAQLQ
                     QQKIGTERNFRPVADYSMPNVSDKVVRIILSYTDYVKRVVWGEECSS"
     gene            complement(2184..3326)
                     /locus_tag="PK049_01150"
     CDS             complement(2184..3326)
                     /locus_tag="PK049_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOK60191.1"
                     /translation="MIKVGITGQSGFIGTHLYNTLGLYPDRFQRIAFEDSYFDDPIKL
                     NGFVQSCDAIVHLAAMNRHNDPQVIYQTNINLCQKLINACEATRSKPHIIFSSSTQEE
                     RDNLYGKSKREGRLLFDQWARRNHTAFSGFIIPNVFGPFGKPYYNSVVATFCHQLTHN
                     EEPKIDVDGELRLIYVSELVEHFITKIDSVAQSRNAVSHVETVQIPHTATIKVSALLA
                     RLNDFKSNYFDKGIFPNLTDPFERNLFNTFVCYIDHEKFFPRMLELKTDDRGSFVETV
                     KLNSGGQVSFSTTKPGVTRGNHFHTRKAERFAVIKGKAVIELRRIGTDKKLTFELDGD
                     RNPSVVDMPIWFTHNIKNVGNDDVYTIFWISEHFDPSDTDTFFETV"
     gene            complement(3392..3763)
                     /locus_tag="PK049_01155"
     CDS             complement(3392..3763)
                     /locus_tag="PK049_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005784536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HOK60192.1"
                     /translation="MEINQITHEIIDSAYKVHSTLGPGLLESTYQACLAYELQKKGFQ
                     VEIEKPVPLIYEEIKLECGYRLDLLVENQVVVELKSVDAFTDVHIAQVLTYLKLANKH
                     IGLLINFNTKSLKDGIKRLVL"
     gene            complement(3821..4870)
                     /locus_tag="PK049_01160"
     CDS             complement(3821..4870)
                     /locus_tag="PK049_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HOK60193.1"
                     /translation="MPKSIFDNKSLLITGGSGSFGNAVLRRFLKTNISEIRIFSRDEK
                     KQDDMRRFYNDKRIKFYIGDVRNEGSVRSAMVGVDFVFHAAALKQVPSCEFFPVEALK
                     TNALGTDNVLDAAEKYHVKRVVTLSTDKAVYPINAMGITKALMEKIMIARSRNNDPSK
                     QVFCGTRYGNVMASRGSVIPLFVEQIKAGQPLTITDPNMTRYMMTLDDAVDLVLFAFE
                     HGQPGDIFVQKAPAATIETLAKALLELYHAKNPIKVIGTRHGEKLYETLLTREEMVKA
                     YDMGKYFRIPADNRDLNYGLYFSEGQTDITQIEEYNSHNTRRLDVEGMKELLLKLPLI
                     RKDILNEDNGHIYEP"
     gene            complement(5140..5970)
                     /gene="lgt"
                     /locus_tag="PK049_01165"
     CDS             complement(5140..5970)
                     /gene="lgt"
                     /locus_tag="PK049_01165"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOK60194.1"
                     /translation="MLAYITWNVNPEIFSIGPFSVRYYGLMWAMAFYIGYVIFNRFVK
                     REHLPEGFLDSLTMYMIVGTVIGARLGHCLFYEPEFYLSNPLEILKIWHGGLASHGAA
                     IGILIALYLFARKHKVPMIYVIDRVVITVAIGGAFIRLGNLFNSEIYGVETTLPWGFI
                     FVRNGEVHPKHPTQLYEALSYTAIFILLYWLYLKADGKLRNGFIFGIFLVLLFGARFL
                     IEYVKEPQVEFEKHMMLNMGQWLSLPFIAAGIGFLIYSYLKPSVFIPQDNVKPKKGNK
                     "
     gene            6679..6867
                     /locus_tag="PK049_01170"
     CDS             6679..6867
                     /locus_tag="PK049_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60195.1"
                     /translation="MQSFTVALWKNIACIAIHMGVAMELQMLRPYGAGKVVRGIDLTP
                     APLLTGEGRCNKETLLQG"
     gene            complement(7516..8784)
                     /gene="metK"
                     /locus_tag="PK049_01175"
     CDS             complement(7516..8784)
                     /gene="metK"
                     /locus_tag="PK049_01175"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOK60196.1"
                     /translation="MGYLFTSESVSEGHPDKLADQISDALLDEFLRRDPNSKVACETL
                     VTTGLVVLSGEVKTHAYVDVQEIARRVIRRIGYTKHELRFDAESCGVISSIHEQSPDI
                     NRGVERGDEFSQGAGDQGMMFGYACRETDDYMPLALVLSHVLLMELAKIRREGKQMAY
                     LRPDAKSQVTIEYDDNNQPLRIHTIVVSTQHDEFDTDDVRMLNKIKEDVRTILIPRAK
                     ARFPERVQKLFKDDYILHVNPTGKFVIGGPHGDTGLTGRKIIVDTYGGKGAHGGGAFS
                     GKDSSKVDRSGAYAARHIAKNMVAAGIADEVLIQVAYAIGVAEPVGLYVNTYGTSHVK
                     LTDGQIAEKIKQIFDLRPGAIVKRLGLKNPIFEETAAYGHFGRDYYKREVEFIVNGNC
                     EGEKVVKREVEFFTWEKLDYVDILRKEFNL"
     gene            complement(8965..9969)
                     /locus_tag="PK049_01180"
     CDS             complement(8965..9969)
                     /locus_tag="PK049_01180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOK60197.1"
                     /translation="MKSIVKSRLFNFAVFFSLAIVLLYYAFRSVDFTHIAQGFREVNY
                     FWIAISLLIALIAHALRAIRWGYLMESLGHKPPFSNLFAAVMFGYLANLALPRFGEVA
                     KCGSVSRVSRIRFESLVGTVVVERAADLAMLLIATVIVVLVKIDSFGQYIYTKIVHPI
                     EQKAIHIEGYKIVILLVMAIAFLLLIRFILKSDFLGQKLNGKIKSAVIGVIDGLKSIY
                     RTPRVIQFALLTVLIWVSYWLMTWALLCSTPITSNLTGWDALFIMVIGSYGMTVPVQG
                     GFGAYHIITASALGIFGITYENGLIFAIISHESQTIMVVIAGIVSMVYLYIKQRKPLA
                     "
     gene            complement(9987..10337)
                     /locus_tag="PK049_01185"
     CDS             complement(9987..10337)
                     /locus_tag="PK049_01185"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOK60198.1"
                     /translation="MMIEVCKSKIHRVTITEANLNYIGSITIDEDLMDAANIIENEKV
                     QIVNINNGERLETYVIKGERGSGQVCLNGPAARKCVVGDVVIIISYATMDFEEAKKFK
                     PTVIFPDTATNKLV"
     gene            complement(10359..11204)
                     /gene="panC"
                     /locus_tag="PK049_01190"
     CDS             complement(10359..11204)
                     /gene="panC"
                     /locus_tag="PK049_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOK60199.1"
                     /translation="MCVVVDKISDLKTMLQGFRSEGKSIGFVPTMGALHKGHVSLVER
                     SVAENDITVVSIFVNPTQFNDKNDLKNYPRMPEKDIAMLEAAGVNVVFMPTESEIYPE
                     PDTRVFDFGMLDKVMEGKFRPGHFNGVAQVVSKLFDIVEPHRAYFGQKDYQQLAIIRA
                     MVRMLGYRIEIVGCPIVREPDGLAMSSRNLLLTPEHRKSAPLIYQTLAEARNKTNEFS
                     VKDMIDWVVNRINSDPNLKVEYFELADADSLLPVQGWDHPNGIVGCIAVWAGNIRLID
                     NMMFK"
     gene            11501..12340
                     /locus_tag="PK049_01195"
     CDS             11501..12340
                     /locus_tag="PK049_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOK60200.1"
                     /translation="MKEKKAKILFISQEILPYLPESEVSKISRFLPQGIQEKGREIRT
                     FMPRYGCVNERRNQLHEVIRLSGMNLIINDMDHPLIIKVASIQSARMQVYFIDNEDYF
                     QRKSTLTDENGETFDDNDERAIFFSKGVIETVKKLRWSPDLIHCHGWFTAFAPLMIKK
                     AYADDPLFAHSKVVFSVYNDGFSKPLNENARKKVLMEGISKKDVEILREPTYENLLKL
                     AVNFSDGVILGSPTVNGQVLEHINTAKKPLLPYQDSENYIDIYDEFYNQILNGNSNVS
                     KKK"
     gene            12321..13625
                     /locus_tag="PK049_01200"
     CDS             12321..13625
                     /locus_tag="PK049_01200"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 family protein"
                     /protein_id="HOK60201.1"
                     /translation="MFQKRSSIGIRHLGVLISGLFLSIFSLTSCYNEPGFLGNNLLPD
                     EDIYKVKIDSSFKVSAFTLTKDSLNSFLASEGVMGYVNSEIFGSTKASFVGRYLPGKS
                     TDGYGGPTATADSIFFYFTASGFYGDSTTELNLRIHEITDTSLFWDKQNALSPINVNT
                     YNPTPFVTATLKGKGSLKLPLPLAFGQSLMDSLALTDSKIFYTKFKGFYVTFDELPGY
                     GGVAYNVSTSNMYLRLYYHYVKQIDGKDSTIKTSKTYTFGSRFYEYLHDYTKADPAKK
                     IQHLNDTITQDTVFYTQSLGGVYGKITFPDLTQWRDSMPIVIHRAELIIGKADATTSL
                     PDSTISQLLFYYKDGNEWIGLIKDQITSTNTLSSNGSYRTFRNGYSVNITYHFQRMLK
                     GEYSDNSLYVFPNSNTTIRHGVLKSGQNSTPIKLKITYTKLK"
     gene            13627..15462
                     /gene="glmS"
                     /locus_tag="PK049_01205"
     CDS             13627..15462
                     /gene="glmS"
                     /locus_tag="PK049_01205"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOK60202.1"
                     /translation="MCGIVGYIGYRQAFPIVINGLKRLEYRGYDSAGICIVNGDPFLA
                     KQKGKVSDLEAFIGDRPLPGTIGIGHTRWATHGEPNDINAHPHESMNGKFILIHNGII
                     ENYSLLRSKLEKRGYIFKSETDTEVLVNLIEYIYLKGNVTPEIAVRLALTKVVGAYGI
                     VVINRDEPDTLIAARKGSPLVIGVGEGEYFIASDATPIVEFTKSVIFLNDNDVAVLQR
                     NQMVLKTIYNQALEPNIQQIDIDIEAIEKGGYEHFMLKEIFEQPRSIHDTYRGRISSD
                     PFEIHLGGLYSVLPNLTEAKRIILIGCGTSWHAALVGEYLIEDLARIPVEVEYASEFR
                     YRNPIINKDDVVIAISQSGETADTLAAIRLAKEAGALALGICNVVGSSIPRETHAGVY
                     THAGPEIGVASTKAFTAQVTVLIMMAILLGRRRGTLTEERYRELIREVTLVPDKIEKI
                     LQSEELVKVISERFKDSRNFLYLGRGYYFPVALEGALKLKEISYIHAEGYPAAEMKHG
                     PIALIDDTMPVVVIAIKDSSYEKIVSNVQEVKARKGVVIAVVTEGDTTIKEMADYTIE
                     IPEAGEIVGALLTVIPLQLLAYHIAVMRGCNVDQPRNLAKSVTVE"
     gene            complement(15510..16394)
                     /locus_tag="PK049_01210"
     CDS             complement(15510..16394)
                     /locus_tag="PK049_01210"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOK60203.1"
                     /translation="MIKQLKFPLTFVLIGTLVLSCAVSKKNKQLSDEAKRAFEMNDYQ
                     AALASYEQLIATGKGVTGDVWNKAGIAAWEVGQTEKAIDYLEKAKKENSADAQGLFAL
                     AKAYRKIDNLSREIVNLKLCVDLNQPELLSQARATLFDAYIRSENWNLADSLWNALAP
                     QYQSDVNLKTGYLIVNRKLKNNQKAEMLAKELLKHDKNNTEALEFLGEYHFNRADELY
                     VSEMNAYEKNKTTKQYKQLTEALKKVNADYKISRDYFETLYRLKPDKRYARYLGNIYT
                     RFENKQKADYWYKLAKYK"
     gene            16539..18125
                     /locus_tag="PK049_01215"
     CDS             16539..18125
                     /locus_tag="PK049_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60204.1"
                     /translation="MKKLIIAIAITLASPYLYAQETYHHISNIGIYEFIDELNTLGIV
                     NVNTSVKPYSRDFIKTLLTQANEQKEKLNNRQRKELEFYLADFDKDNAAKGKKRFDIL
                     YRSDSLYSITINPLLGVNTIKNDSAFAYNRWVGAEVHGHAGRFGFYASLRDYAQNYRL
                     AMPTYLTREQGGNYKVDGDGGEFSEMRGGITYGWKWGSIGMIKDHVQWGSGYNGTNIL
                     GGRTPSFAMLKLNVKPARWIELNYIHGWLVSQVIDSARTYISFGETRKVYHNKYIAAN
                     LITFKPYRGLNLSVGNSIVYSDLEVQPAYLIPFFFYKSIDHHLNGMSNQVGQNSQMFF
                     DISSYNVKHLHLYATLFLDELSMKNMFNPDKHSNFYSLKAGFRVWDLPLSNLSLTFEY
                     TRTNPLAFKHIIETTTYESNRFNLGHYLTDNADEIHFALSYHPTRGLQLFGSFTKSRK
                     GPDYTKITTERLGLPFMEQVEWEQTFWQVGARYQIINDGYIFIAGGVNNIKNYTSYKY
                     TPNFMLGKQMQVEAGICWGF"
     gene            complement(18122..19639)
                     /locus_tag="PK049_01220"
     CDS             complement(18122..19639)
                     /locus_tag="PK049_01220"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOK60205.1"
                     /translation="MNRRVSILILLDIFLIGVSFWLTTVIKGVSFQTYLGNYGNGVLI
                     FTLTWILTSLIYNKYSFTSYSIRHLSKNIITSNLIVLAIISILIFLTRNDYYSRFIVF
                     STIAFTTLAELFIYNLWGVLKKTQVLPDDVLGKVERSLRRPRPAPTISEEPIDPTRVK
                     TVQKAILSDFSRDVYCFLEQHTNLFSDKTLIIATTTSFNLLNQPSNTFKTIINLKRVN
                     DIRRINKFFEAANEKIPVGGVFVCMAETQEQRKKRILSKFPPILNYIYYLFDFILKRV
                     FPKFSFTKGIYFLLTRGENRVISRAELLGRLYSCGFDVIDEQEIDKMHYVVSQKIRKP
                     YFDMEPTYGPLIKLRRIGKGGKFIKVYKLRTMHPYSEYLQEYIYRKHNLQEGGKFKDD
                     FRVSTLGRFMRKLWIDELPMIINLLRGELKIVGVRPLSQHYFNLYSKELQERRIKYKP
                     GLIPPFYVDNPKTLEEIMASEIKYLDAYDKHPLFTDLKYFFVAVYNIVFKRYRSN"
     gene            19786..20991
                     /locus_tag="PK049_01225"
     CDS             19786..20991
                     /locus_tag="PK049_01225"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005924263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HOK60206.1"
                     /translation="MLSIREIYKEGYGPSSSHTIGPARAAEIMKQRHPEANYFRVSLY
                     GSLALTGKGHHTDSALRKSFLPKQVDIIWRPEESLPIHPNGMIIEAFSEEDGQLIDFW
                     EVYSIGGGDLKDKTGVLNNNPVYRLTTMTEILAWCKSEGKSLWEFVEDNEGKEIWDFL
                     AKVWHTMRETVKRGIDNEGVLPGPIKLARRASAQYTKALNAHGTMKHIGLLCAFALAV
                     AEENAAGGKIITAPTCGSSGVLPGVLFYLNSEEHITEQKMLRALATAGLIGNLVKHNA
                     SISGAEVGCQGEIGTACAMAAAAMTQIMGGSPTQIEYAAEMAMEHNLGLTCDPVMGYV
                     QIPCIERNAIAATKAYSCAVYALSSDGGHKVSFDKAVETMAETGRDIQVAYRETGIGG
                     LAKNWEAFG"
     gene            complement(20988..21956)
                     /locus_tag="PK049_01230"
     CDS             complement(20988..21956)
                     /locus_tag="PK049_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOK60207.1"
                     /translation="MAEVVAGIDIGGTNTVIGIVNRNGDILAESTLPTRCNATFDVFV
                     KNLTGAIEQLLMELGHEHRLLGVGVGSPNGSYNLGAIVDAPNLEWKGILPLCKEITLL
                     TGVPSVVTNDANAAALGELLFGAAKGMKNFVVITLGTGLGSGIVVDGKLVVGHDGFAG
                     EFGHVVAKANGRQCGCGKKGCLETYASATGLRRTAFKMIADSNQPSMLRNVTYDKLTA
                     KMITEAAKTGDPLARAAFEYTGLILGTRLADLVAILNPEAIFFFGGLANAGELLMDPV
                     RRYMEDYMFPVFKGKVKLLLSGLQDKNAAVLGAAALIWEELDKSAH"
     gene            complement(21981..22214)
                     /locus_tag="PK049_01235"
     CDS             complement(21981..22214)
                     /locus_tag="PK049_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60208.1"
                     /translation="MIAFLKFIFYFFLTLFLLGLIGRIILRLWLRRLYKKINQETGQQ
                     SSDNSKGRNFSFTRKKKIIDKNQGEYVDYEELD"
     gene            complement(22248..24935)
                     /locus_tag="PK049_01240"
     CDS             complement(22248..24935)
                     /locus_tag="PK049_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60209.1"
                     /translation="MKRIIATIVFLTLAIAGLVFLFYHWVNKQDYRQFNPIDAIPLNA
                     SLILNIDDFELFTNSVKSNGWWKTLERGDVVKSFADMLNYLDSAAKSDNTVRTAIKGT
                     NTAVAFYNNHPQGSEFLLAHKVPKEINQSDILELIKLQSLGYAKAEEIQIAGNSAIYV
                     EVPEGNYFSLTFTVFGNLLLVSNSQELLVESVKKLKEDAAVESDEQFQSLYQTAATGV
                     AANVFVNINELSKTLSLENNSLALKGVASWIGLDVQVSPTMVIANGLIVSSATNGDYY
                     KIFTRQSPVPFSLHGYMPASTSFFTWYGLPNIPNFMADYSDYIDNLGLTEPYNRNLSG
                     FLAHTGVSIDEFLVKNLDNEVAVLSAKLQNDSTDWFILANTKSGSTTLQQLESFAGTK
                     TIVKHEFKPDQQKSFPIVENPIKWFIPTLLGNSFLKVNDEFVTVIDNVLVFGSSISSL
                     EFIINEYLRNNTLTRTEGYRSIQQRISTSSNLILYSKGMDSYPLSELHLHGKPTQLYK
                     GKTLSSHNFIWQVVGGSQKLFAMLAIKSSQADLTETPNSLKNVNWVCKLSTEPVGKPH
                     LLVNHLNGQPEVLVQDSENSIYLISNDGHILWKRKIGAPILGSVSQVDIYRNKKLQMA
                     FAAGNRIYLVDRNGNDVEGFPVTLPARATSPLSAFDYDSNRNYRFIVACSDKKIYCYD
                     TNGKPVSGWLSFKTETVVSSRIGYVSNQGKDYILIFDQNRPYLLNRRGEERLKPQSFF
                     AKAKNSEFYLVGNSGKGSYLLTTDTLGIIKKLFFDGRVESQVLEPYSPNHTFIMAGKN
                     YFILDRNRVSIYSNEGKLKNAILSSIGDFDTGNLQVMPNNNLVLTNRDESIFGYDFSG
                     KPLSFFPISGNIPIVEVNSRNKQFVTLSKQNGVICFAIK"
     gene            25197..28640
                     /gene="ileS"
                     /locus_tag="PK049_01245"
     CDS             25197..28640
                     /gene="ileS"
                     /locus_tag="PK049_01245"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOK60210.1"
                     /translation="MGNLKFKEYKGLDLPQVNKDVLKTWEQQNTFKKSIETRKGHPKF
                     IFYEGPPSANGIPGIHHVMARTIKDIFCRYKTQKGFLVERKAGWDTHGLPVELGVEKM
                     LGITKEDIGTKISVEEYNNTCRKEVMKYTDLWEDLTQKMGYWVDMDDPYITYDNRYIE
                     SLWYLLQKLFEKGLLYKGKTIQPYSPAAGTGLSTHELNQPGCYRDVKDTTVVAQFNVI
                     RNESSQFLFDLTDTPDVYILAWTTTPWTLPSNTALAVNASIKYSIVRTFNPYSGEPLT
                     VVLAKDRQDYYFPAANAELSFNDYQPGDKKVPFKVLSEVEGSKLVGIRYHQLFPWVKP
                     EGDAFRVIAGDFVTTEDGTGVVHIAPTFGADDDRVAKASGIAPLILRDKYRNPEPMVD
                     RRGRFYPIEDLDPDFVKSNVNVDLYREFAGRFVKNEYDSSLPEDAPTLDVDLAVALKK
                     ENKAFRIEKHVHNYPHCWRTDKPVLYYPLDSWFIRVTALKDRMIELNKTINWKPASTG
                     TGRFGKWLENLLDWNLSRSRFWGTPLPIWVSEDRSEMICIGSVAQLKDEIEKAIKAGF
                     MDKNPLANYKEGDFSKENYNSFDLHRPFVDNIVLVSPSGKRMFREPDLIDVWFDSGAM
                     PYAQIHYPFENKDNLNELFPADFIAEGVDQTRGWFYTLHALSTLLFDSVAYKNIVSNG
                     LVLDKNGNKMSKRLGNAVDPWEVIEKYGSDPLRWYMITNAQPWDNLKFDIDGVDEVKR
                     KFFGTLYNTYSFFALYANVDGFSYAEDEIPVSQRPEIDRWILSLLNSLVKEVEEAYED
                     YEPTRAGRAIQDFVIEQLSNWYVRLNRKRFWGGEYDTDKIAAFQTLYTCLETVAMLAA
                     PIAPFYMDQLFTDLNRVTHRHSAESVHLANFPKCNDELIDKNLEEKMEMAQSICSLVL
                     GLRRKVNIKVRQPLQKIMIPILDERFREQLAAVENLILSEVNVKELEYLSDSSGILVK
                     KIKPNFKTLGPRYGKAMKAISEAIAKMTQEDIAAFERNGKFTIKAAEGDINLTLEDVE
                     ILSEDIPGWLVANEGRYTVALDITITEELRMEGIARELINRVQNMRKDSGFEVTDKIR
                     IKIQKHDKLNRAIEVHKSYISTQTLAREIILVDEELNGNSNVIELDDELSTAISVEKI
                     E"
     gene            28696..29073
                     /locus_tag="PK049_01250"
     CDS             28696..29073
                     /locus_tag="PK049_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOK60211.1"
                     /translation="MGEKTRYSDEELAEFKEIILQKLEKARRDYEILKSAITMDESND
                     TEDTSPTFKVLEEGASTLSKEEAGRLAQRQLKFIQHLEAALVRIENKTYGICRETGKL
                     ISKERLRAVPHATLSIEAKNSQR"
     gene            29163..29822
                     /locus_tag="PK049_01255"
     CDS             29163..29822
                     /locus_tag="PK049_01255"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005865261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HOK60212.1"
                     /translation="MNFNKKHLAFLIILLILVIDQALKVWIKTNFAIGDEMRVIGNWF
                     ILHFTENPGMAFGLKFWGIWGKIGLSIFRIAAIVAIAWYLNHLIKQQVKTGIIVGLSL
                     ILAGAIGNIIDSAFYGYLFDSGTTYSSEFNQWVSYYGISKLNFGGYAGFLQGCVVDMF
                     YFPIIDTVLPTWVPIIGGEHFVFFRPVFNIADSSITIGVIYLLLFHRNTLFGHQPNQK
                     P"
     gene            29829..30299
                     /locus_tag="PK049_01260"
     CDS             29829..30299
                     /locus_tag="PK049_01260"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034023 - 5-(carboxyamino)imidazole
                     ribonucleotide mutase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOK60213.1"
                     /translation="MNRKVVIIMGSKADLDWANQIVNVLKGFGIESVTRIASAHKVPL
                     KCYELIKHYEKENVVFITIAGMSNALSGFTDAQTHCPVIACPPYSDKFGGADVYSSLR
                     MPSGVAPLTILSTENAALAAAKIIGLSNPDIQKKIAEYQEKKRIEIEEADKNIQ"
     gene            30309..31256
                     /locus_tag="PK049_01265"
     CDS             30309..31256
                     /locus_tag="PK049_01265"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOK60214.1"
                     /translation="MGLVGVDIDHAASIIANGGVVAFPTETVYGLGANALNPNAVAKV
                     FALKERPSFDPLIVHIASAESINLLSSSNDPRVFQLAERFWPGPLTIVVPKSTVVPGI
                     VTSDLDTVGIRMPNHPIALELIKKSGCVIAAPSANKFGRVSPTRAEHVLKQLPEVDYI
                     LDGGRTTVGIESTVITLDEEGFIILRHGAITHNHLAQVLKPSGAHRPLKGLASPGLLK
                     SHYSPNKPLYIAGETSKPIDPSRAGYIAFGKKPEGNYKRVEYLSETGDMVEAAAHLFE
                     KIHAFEDSDVEYIVADPVPEVGIGIAIMDRLRKAAYRYK"
     gene            complement(31362..31952)
                     /locus_tag="PK049_01270"
     CDS             complement(31362..31952)
                     /locus_tag="PK049_01270"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOK60215.1"
                     /translation="MYHIPRTRFWNWDDNPVSQIFWGRVNVEHACSFFYFRKGSRYRK
                     LLHMLKYQGREDIGVFLGRQFGLELKSSELYKPITAIIPVPLHPKRFKERGYNQAEAI
                     ARGLSEAMGVPVVTDVLLRNIYTQTQTKKTRMERMQNIAGAFTICNPEKIEGGHVLLV
                     DDVITTGATLETCTQTLIDSVNVKVSVGTLAYATNT"
     gene            complement(32067..32504)
                     /locus_tag="PK049_01275"
     CDS             complement(32067..32504)
                     /locus_tag="PK049_01275"
                     /inference="COORDINATES: protein motif:HMM:NF021438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HOK60216.1"
                     /translation="MKKLFLLSLAIMLIVPAMAQHQDKVLGTWLTQYGDSKVTIKKDA
                     KGKFYGEITWLKEPNKDGKPKVDDKNPEPKLQNRPIMGLRMLDGFVYKDNQWVDGTIY
                     NPKEGKTYKCYMWFDDGNYNLLHVKGYIGFSFIGKEVEWTRSN"
     gene            complement(32553..33305)
                     /locus_tag="PK049_01280"
     CDS             complement(32553..33305)
                     /locus_tag="PK049_01280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HOK60217.1"
                     /translation="MRSLFTAIIALLPICINAQVGYVQEGNASYYGDNFEGRVTASGE
                     RYSHLKATCAHVSIPFGSLVKITNLDNNQTAIARVNDRGPFVPDRIIDVSKSVAERLG
                     MIGTGIARVRIEVVDNQGIKLNSQEEVSKTAVTDGDKKTTNLQTTEIKTNQQAAPSEA
                     IEYFSVEVQKQTKKGFYVQLGSFREQTNIFLLLADAQEQFGKKTCVQVVAVNNDRLFK
                     LLIGPFTSRSDAEKVKQSAQTKYPGCFIVEAK"
     gene            complement(33307..33969)
                     /locus_tag="PK049_01285"
     CDS             complement(33307..33969)
                     /locus_tag="PK049_01285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HOK60218.1"
                     /translation="MPGLQKETVNFLVNLSNNNNREWFQVNRNYYKLAQTDFQRLVFT
                     LIAGISHFDKSIGALEPRDCIFRIYRDVRFSTNKNPYKENFGAFIAPGGRKSSGCGYY
                     IHIQPGGSFASGGLYMAPAPVMKAVRTAMVENRSEFLSIVNNPKFKSTFTWEGVEQAK
                     RTPAGFEFNPELDLFLRLKHITPSRDFTDDELFHPDFDLRAIEVFETLSPLVKFLNQA
                     IP"
     gene            complement(34051..34123)
                     /locus_tag="PK049_01290"
     tRNA            complement(34051..34123)
                     /locus_tag="PK049_01290"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34088..34090),aa:Lys,seq:ctt)
     gene            34239..35297
                     /gene="rfbB"
                     /locus_tag="PK049_01295"
     CDS             34239..35297
                     /gene="rfbB"
                     /locus_tag="PK049_01295"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOK60219.1"
                     /translation="MERRTILITGGAGFIGSHVVRRFVNSYPNYLIINFDKLTYAGNL
                     QNLTDIEGKSNYKFIKGDIASEAHVDKVFSEYAIDGVIHLAAESHVDRSITDPMAFIR
                     TNILGTTILLNAARKFWNGNWEGKLFYHISTDEVYGSLGAEGFFTEETPYDPRSPYSA
                     SKASSDHLVRAYNHTYGLPAVISNCSNNYGPNQFPEKLIPLSIHNIKNSKPIPIYGKG
                     ENVRDWLYVEDHAAAIDLIFHKAKPGTTYNIGGNNEWKNIDLIRLLCQIMDRKLGRAQ
                     GESEKLITFVKDRAGHDLRYAIDSSKIQRELGWKPSLTFDVGLEKTVEWYLANEQWLN
                     NVVSGDYLKYYEQQYGGR"
     gene            35625..36071
                     /locus_tag="PK049_01300"
     CDS             35625..36071
                     /locus_tag="PK049_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOK60220.1"
                     /translation="MAEKPDSRIVEFINEHHVLTLATSFNEEPWCANCFYVYMDDENS
                     LVFTSDFDTKHIQQASHNIYVAGTIVLETSIIGKIQGVQFQGIISQPQGELHERAKKA
                     YLKRFPIAMLMETHLWVVDLTYLKMTDNRLGFGKKLIWSKDLSFQK"
     gene            36086..37075
                     /locus_tag="PK049_01305"
     CDS             36086..37075
                     /locus_tag="PK049_01305"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOK60221.1"
                     /translation="MILVTGATGLVGAHLLYELCSGGHKVRATRRANSKTHFVEWVFS
                     LYTANSSDLLNLIEWVDAEVLDYQSLVEATKGVSTVFHTAATVSFSPSQEQNILATNV
                     RGTANVVDACLENGVKTLCHVSSIAALGERNDLGVIDESCKWQKSKGQSAYARSKFLG
                     ENEAWRGFEQGLRVVIVNPSVILGPGRWNSGSGQLFSIVSKGMPFYTDGVTGYVDVRD
                     VAKAMVFLTLNTEVSGERFVLNSENLGFRELFTLIANVSNNRKPFIKIPLALIHVLYP
                     LIFILTAGRISKSNLKSAFTKSYYSSDKIKALGFTFTPITQTVQLMAEAKKKS"
     assembly_gap    37265..37364
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            37504..37983
                     /locus_tag="PK049_01310"
     CDS             37504..37983
                     /locus_tag="PK049_01310"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HOK60222.1"
                     /translation="MDKREILSKKILSGVEYDRMVAFWNFKSRSVVALYGTFDVFHPS
                     VFDLITFASELGQELAVGVKPDTDVKAEKGEKFPIYNQDQRAAMVSAHQLVSAVHICN
                     EGVEDFLRRTKPAVAVCCAHASETDKKALEVVKEWGGRVEIFTGSTTSIDDIISKYK"
     gene            37984..39363
                     /gene="radA"
                     /locus_tag="PK049_01315"
     CDS             37984..39363
                     /gene="radA"
                     /locus_tag="PK049_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOK60223.1"
                     /translation="MAKTKSVYVCQNCGAESVKWLGRCPSCNEWNTFVEERITKESKS
                     RPGLVEVSRNAIPKPLKEIETSNEKRIDTRIGELNRILGGGIVPGSIVLLGGEPGIGK
                     STLALQLALGMDHQKVLYVSGEESARQVKIRADRINPANQDCLILNETLLENILTQIE
                     NVQPDLIVIDSIQTLYTDTIESSPGSVSQIRETAATLLRYAKTSGVPVILIGHITKDG
                     SIAGPKVLEHIVDVVLQFEGDQNYLYRILRSSKNRFGSTSEIGIFEMQNSGLVEVTNP
                     SEILLSHRDEQLSGIAVAATIDGARPFLIETQALVSTAVYGTPQRSTTGFDQRRLNML
                     LAVLERRAGFRLANKDVFLNIAGGLKVSDPAIDLAVIAAILSSTLDVAIPGTTCFAAE
                     VGLSGEIRPVNRLDQRVAEANKLGMTRMFVSRYNTKGLDNGNKNCKIIPIGRIEELIR
                     LLFGRESVD"
     gene            39375..40643
                     /locus_tag="PK049_01320"
     CDS             39375..40643
                     /locus_tag="PK049_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60224.1"
                     /translation="MRIAVIGFGAAAIGLLEKLKNSNHEVHVFEKSNDIYSSSISGIR
                     ADGKLFVSTEMGGEIYIDPLLQHRFVEYYINFTDKREVETGNSFASEDYYRKFYEKGF
                     LPISSQFFHIGTDQLKEVLFNIYEQFCKSANIHFHFSTEVTEVKEQPDGVIVNGTERF
                     DVAVIAVGRSGHKLINRTISEYPQLIKNNTKVDLGIRYELPNHIVDEINREMYEFKVK
                     YKSRTGYMVRTFCNNPSGFVVTENYGDFTTVNGHAKMREKSGNTNFAILASLVLTEPF
                     NDPIGYGSYIAKLSNLLAGEGKVILQTYSHFKESKRTKNLYRVHPTLEPEKYILGDIN
                     LAFPRRIIESIIDFIENLNTVIPGVANNDNLVYAPEIKFYSNRLNNQISARLKFIGDC
                     SGATRSIIYATAHGYLMAEAIQSNNFNVDI"
     gene            40643..42235
                     /locus_tag="PK049_01325"
     CDS             40643..42235
                     /locus_tag="PK049_01325"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK60225.1"
                     /translation="MLKVSDLTVSFSGNPVVSGISFSLMPGEILGFVGESGSGKSVTS
                     LALMGLLPPNAKVEGGSASFSTEKGQVDLLKVDSKTHRQIRGQGIAMIFQEPQTSLNP
                     SMICGKQISEAVELHSNLRGAKAKERCIQLLAEMQLTDPAKVYSSYPHQLSGGQKQRV
                     MIAMALAGNPRILIADEPTTALDVTVQKSILQLLKDIRQRHQIGIIFITHDLSVVAEV
                     ADRIMVMQKGQIVEQGAVTEIFNNPTHPYTRRLIEFRKRINQPVTTTASVTNEPEPIF
                     RVNSINVEYGSNSWFKKGFKAISDVSFTLFKGETLGLVGESGSGKSTIGRTLLKLIET
                     QSGTIEYNREPLSSLKGSNLLNFRREVQLIFQDPYSSLNPRLTIGQAIMEPFIYHGLG
                     GKREARKAVEELLQMVSLPADSINRYPHEFSGGQRQRIVIARALVLKPKILVCDEILS
                     ALDVSTQAQMLDLLQELKKGMGLTYLFISHDLSVVRSICSRVIVLNRGSIEEQGPAEE
                     IFSRPQSVYTQLLIDAIPGKSL"
     gene            complement(42232..>44076)
                     /locus_tag="PK049_01330"
     CDS             complement(42232..>44076)
                     /locus_tag="PK049_01330"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOK60226.1"
                     /translation="TAVKLERNYTKEEIAAMYLNVVEFGSNSFGIKAAAKTFFNTTPD
                     SLTIDQAALLVGVVNAPTRYSPVRNPENAKQRRNVVLGQMLKYGYINQEQFDSISKLP
                     IVLKYQAQDHNQGTATYFREMLRLFMTASKPNPKRYWSQQLYREDSLLWETNPLYGWC
                     NKNTKPDGTPYNVYTDGLKIYTTVNSRMQRYAEEALAEHLKNDLQPALDKEIKARGGR
                     IFYDITPEQADHIIYLAMRQTQRYRGLVASGASRDSIMANFNEKIPMRVFSWKGERDT
                     LMSPLDSIRYYKRFLRSSFMAMDPHNGHVKAYVGGPDFKHFKYDMVRQGKRQVGSTIK
                     PFLYTLAMQEGYSPCTKVPNVTQTFVVGDTIWAPKNSGSTKYDGQMVTLKWGLSNSVN
                     NISAWIMKQFAPSAVVEMIHQLGIKSYIEPVPSIILGTFEFSLYEMVGAYGTYVNKGV
                     QVEPIFVTRIEDKNGNVLATFNTKKNEAISEQTAYLMVNLLESVVNQGTGVRLRLKYQ
                     LPGKIGGKTGTTQHHSDGWFMGVTPNLVAGVWTGAEDRSVHFQNLALGQGANMALPIF
                     GLFMQKVYADPSLGISPNDDWQRPILPGNISINCENETNYEINEIEIY"
     assembly_gap    44077..44176
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<44177..44421)
                     /locus_tag="PK049_01335"
     CDS             complement(<44177..44421)
                     /locus_tag="PK049_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HOK60227.1"
                     /translation="MKLNDPATIQKFKTWFWGALAFGFLLVVTIFILIGLEFFGPLPT
                     FKELENPKSNVASEVISDDNIVLGNIYKEYRSFVDYN"
     gene            44725..47052
                     /gene="topA"
                     /locus_tag="PK049_01340"
     CDS             44725..47052
                     /gene="topA"
                     /locus_tag="PK049_01340"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009016940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOK60228.1"
                     /translation="MVPNLVIVESPAKAKTIEKFLGKDFVVKSSFGHIRDLSKNKLGV
                     DIKKNYKPQYIVSDDKKKVVEELKKAAEQAQTVWLASDEDREGEAIAWHLAEVLKLDP
                     QKTKRIVFHEITKNAIQQAIQNPRSIDENLVNSQQARRVLDRLVGFEISPILWKKVKP
                     ALSAGRVQSVALRLIVEREREIINFKSTSFYKVIADFDAQTPFKAELSDKLNTIDEAR
                     KFLEHCKDATFKVLDVVKKPAKKSPPPPFTTSTLQQEASRKYGFSVSQTMTLAQKLYE
                     AGYITYMRTDSVNLSDLALATSREVVLKEFGEKYSHTRQYKTRTKGAQEAHEAIRPTY
                     IDKEKVPGTTAEQKLYDLIRKRTLASQMSDAQLERTTITIEVSGSKYQFVASGEVIIF
                     DGFLKAYSESSDDDSEEASRDLLPLVKVGQALQPKQINALERFTQRPARYTEASLVKK
                     LEELGIGRPSTYAPTISTIIARGYVIKEDRPGIERPYQSITLKQGKITQTQKVEVTGA
                     EKAKLFPSDIGIVVNDFLVEYFPNIVNYNFTAQVEEKFDEIAMGKMEWTKMIDEFYKP
                     FHDTVKKTEKESDYTKGERVLGTDPATGKTVSVRIGRYGPVVQLGDNDPNSKEKPQYA
                     SLLKGQLVSTITLEEALSLFKLPRTLGSFEGKEVVVAVGRFGPYVRHEGKFYSLKKDD
                     NPLAIDLSRAIEIIEEGRLKEKQRVIKTFSEDPELMVLNGRWGPYISKAKSNYKIPKG
                     TNAAELTYNDCLKIIEESEKKETTGKKTTKTTKKK"
     gene            47061..48218
                     /locus_tag="PK049_01345"
     CDS             47061..48218
                     /locus_tag="PK049_01345"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HOK60229.1"
                     /translation="MLANELTAYLVPSDVTGFYPFAEKKYCFGNQVINLHDHPDSPIR
                     PGDIFIVGIESLDSESVSNDIRQMLWGLSSIHYSKGKVFDAGNISATLTLAQQYQVVE
                     SISAIIAESKAHLILLGGSQEFLSKVYAGWKQIQSFIRMAIIDSKIDWDGNNNDFHQD
                     NFVNTLIDEPYEKLLDLSFIGIQGYFTPQHTLQKVLRRKHDYIRLGNLRGNIREAEPT
                     LSDANLVSIDMAAIRASDAPGKAFDNPNGLYAEEICMLSRYAGIGQSSKFLGICGLKT
                     NAPEQTIMLAAQVLWHYIEGVSHRRVENPIKNLPHNKKYVVSTEVQNVEMIFFRSELS
                     GNWWFELPVNSKKKLLRGKLLVRCNASDYSQASNGEIPQRWLRWYQKAQKI"
     gene            48444..49391
                     /locus_tag="PK049_01350"
     CDS             48444..49391
                     /locus_tag="PK049_01350"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HOK60230.1"
                     /translation="MRVFGNLVFATTLSVLLSSHAFGQQDPQFNQILFNPLTINPGYA
                     GSQNNMIGVGAISRIQWAGFGDGAPLSNTLGINAPISPFGFKSGVGLSILNDSYGFNN
                     DLGINIAYAARFKFKLINGDLGFGVGAGFVNNKLDPKWNYPVGGGDEAIPDQKESSIN
                     FDLSAGLYYSTEYMFFGFSALHLNETKTNKAALPARYTRQYYLTGGYIVNFPNPTWQF
                     EPSVIVHSDLKSSKLSLNTIVRYNKKVWGGVSYRVGEAITGLVGFELFNGIKFGYAYE
                     FSTSKISKYNNGSHEFYMGYNFQLNKEKPPQQYKSLRFL"
     gene            49449..50777
                     /locus_tag="PK049_01355"
     CDS             49449..50777
                     /locus_tag="PK049_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOK60231.1"
                     /translation="MKKFLSIGILASLLYGCGPGNTGELTGVPGRRSFSEPTPLGMVF
                     IPMGSFTLGSNDQDVAWAINAPTKTVSVDAFWMDQTEITNNQYRQFVYYVRDSIARRL
                     LGEINDEFLITEDQFGNPLDPPVLNWEVPIDMEDEEQREALNALYYSKEDEFFGRKEV
                     DTRKLFFEYYWIDIKQAAQKRNRYNYETLSYDQGEVINSLGEKVKVDNRQSFIMRDKV
                     NVYPDTLCWVADFTYAFNEPYTNSYFWHPAFDNYPVVGVTWKQATAFCIWRTQTLNKF
                     LAPEGITIQDYRLPTEAEWEYAARGYLNHSMYPWGGPYTRNMEGCFLANFKPLRGNYV
                     DDGGLIPIPVGTYEPNDYGLYDMAGNVAEWTANAYDESAYSFMHDMNPDYKYNSKPSD
                     LPALKRKVVRGGSWKDISFFLQVGSRTYEYQDSAKSYIGFRCVRSYLGAK"
     gene            50814..52055
                     /gene="gldL"
                     /locus_tag="PK049_01360"
     CDS             50814..52055
                     /gene="gldL"
                     /locus_tag="PK049_01360"
                     /inference="COORDINATES: protein motif:HMM:TIGR03513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HOK60232.1"
                     /translation="MKISIEEIVTSKKWKTFMKYLYGWGASVVILGALFKILHLKGAG
                     TMLFLGMGTESIIFFFSAFEPIHEEVDWTLVYPELTGMSDEEEISGYRRSRNQGLSAE
                     DVQQIITSVLASLPAGSAGVAAAAPASAAPASQPAAVGVPGALIFTEKFNKMLENAEI
                     SPALFDKVSAGLNKLGDASSKIADITNSATVVKEFNEKLTKASESVGTFTQNYSQSGQ
                     ALNESINVLNQNIQQAAGVMSESGKNFMDGVNQSVKNLEAELTNAGQKVSERIVGSAE
                     SVADNLKVSADGLANTYKQTASQVATTYQQLAEAMQLNGNVITNGSTSYKEQLERLNK
                     NMAALNAAHELHLQETSQRLKEAEKVYQGVDGMVKKLNFTIEETEKFRLAVEKLNSNV
                     AALNNVYGNMLTAINALSNGK"
     gene            52088..53728
                     /gene="gldM"
                     /locus_tag="PK049_01365"
     CDS             52088..53728
                     /gene="gldM"
                     /locus_tag="PK049_01365"
                     /inference="COORDINATES: protein motif:HMM:TIGR03517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldM"
                     /protein_id="HOK60233.1"
                     /translation="MAHGKETPRQKMIGMMYLVLTAMLALNVSSEVLEAFVLVDNGLS
                     RTTESYRLKNKKLYDEFAAAYSINPVKVGPYKQKADEIQKKATEILQYVQDLKIEVVK
                     ASEGEETPAIQNGEIIAELIQGKSDLDKAATILIGFEGNGKAKVLKQKLEEFREYIVS
                     IIDKDKAPELVESIKKILNTDDPPVKEDGTHNTWESARFEHIPLIAVIPQLTKVQVDI
                     LNVEAEIINYLMGSIGAAEFKFNKLSATVIPNSNIIFQGSEFRAEIFLAASDTTQVPK
                     VYLCRYDSVFNSAINGYDYRPIGNTEEVPVNKTGRAVLTRKASSTGVVNWQGLIEMKA
                     PDGTIVRRPFKHSYTVMPPNVVIAPTKMNVLYLGVENPIDISVPGFAPDKVSATISRG
                     SIKPVGMGSYIAQPGAGAPTVEITVFAETEGKRKVSMGTKTFRVKRVPAPIAKVIGVS
                     GKTVSRSELAAAPGVMAEMPQDFDFDLKFKVVGFKISATVGGFLEEKASTSARITDEQ
                     KIMIRSLSSGTQVAITEIKAVGPSGDVVDLNDLVLKIR"
     gene            53738..54631
                     /gene="gldN"
                     /locus_tag="PK049_01370"
     CDS             53738..54631
                     /gene="gldN"
                     /locus_tag="PK049_01370"
                     /inference="COORDINATES: protein motif:HMM:TIGR03523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HOK60234.1"
                     /translation="MKRILLFSVVILLASSALRAQDRKESLERDGIYTRETTPARVPI
                     PYQFVREADVIWSKRVWRVIDLRQKMNYPLYYPTKLMQDRESLVQRLVRAIKYNEINA
                     YKDEEFTERLSYQQVIEERLGAKDKVEDSLDLEGNIVYGQDGIPLKKIIPGEINWRDI
                     KELLVKEDWFFDKQRSVMEVRIIGICPILHRTDYRKTGSDEEQEGTLSRIPTFWIYFP
                     EARSVLANTDAYNNFNDAQRITYDDLFWKRNFDSYIIRISNVWDNRAIDEYTFTGLQT
                     LLESEKIKTELFNFEHDLWEY"
     gene            complement(54703..55791)
                     /locus_tag="PK049_01375"
     CDS             complement(54703..55791)
                     /locus_tag="PK049_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019149507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HOK60235.1"
                     /translation="MIERKNLYCVIMAGGIGSRFWPLSRVDKPKQFIDILGTGKSLLQ
                     QTFDRMSRVCPRENILIVTSSAYSSLVNEQIPNLSPDQVLLEPLRRNTAPCIAYATYK
                     IKQQNPNAIAIVAPSDHLILNEDIFLDTILKAAEFASNSNALITLGIRPSRPETGYGY
                     IQIGKPVKEVPSLFKVKTFTEKPNLELAQKFLESGEFYWNSGLFIWSIASISAALESH
                     LPEVNSLFKELLPIYGTEKETETIATAYSECRNISIDYGVMEKADNVYVFCSEFGWSD
                     LGTWGSLYTHLPHDPNGNAAIAQQLMLYQTSNSIFNIPKNKLAVIQGLDDYIVVDTKD
                     VLLICKKQDEQQIRNFVNDVLMNNKDFA"
     gene            56077..56826
                     /locus_tag="PK049_01380"
     CDS             56077..56826
                     /locus_tag="PK049_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOK60236.1"
                     /translation="MKLLFAIDMFGEYILLLKKVFSKPEKRRVYLQQTIKEIDKLGLQ
                     SIGIVTIISVFMGAVITLQTAYNMENPFIPNYLIGLGTRDSMLLEFSSTIIGLILAGK
                     VGSNIASEIGTMKVTEQIDALEIMGINSASFLILPKIIATILFNPFLTVLSMIVGIAG
                     GWLVGVFTGVVSTQEYIDGVQYAFIPYYITYSLIKTVVFAFLITTISAFYGYRVQGGS
                     LEVGRASTQAVVMSSIFILLFNLILTQLLLS"
     gene            56823..57572
                     /locus_tag="PK049_01385"
     CDS             56823..57572
                     /locus_tag="PK049_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOK60237.1"
                     /translation="MITVKNLNKSFDGRLVLEGINATFEKGKTNLIIGQSGSGKTVLL
                     KCIVGLHEVDSGEIYYNDTRFDNLNFRDRKKIRQKIGMLFQGSALFDSATVEENVMFP
                     LDMFTTMSHEEKLDRVNFCLNRVNIINSNHLYPAEISGGMKKRVAIARAIALNPEFLF
                     CDEPNSGLDPKTAVVIDQLIKEITEEFNITTIINTHDMNSVMGIGDKVIFIHNGKKWW
                     EGSKENILDSDNKELNDFVFSTELTRKIKGL"
     gene            complement(57574..58272)
                     /locus_tag="PK049_01390"
     CDS             complement(57574..58272)
                     /locus_tag="PK049_01390"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOK60238.1"
                     /translation="MSQNFCPGNICAECGFRSSVFSKLTDEQLNQLTANKTFHFAKKG
                     ETIAKQDSAIKGFIFLRVGLAKLTRVNPDGREQIIGIATPNDFVGLLSIFSSKNYQYN
                     IIAIEDCEYCCVDYNLVINLISTNGDFAKTLLEKISQVADLMMGTRLDLELRQLRGRV
                     AFIICYFGNEVYKSHKFSLPISRREIAELIDMRVENVVRILSEFRRDNIIRIEGTTIE
                     IVDPEKLRWIKMHG"
     gene            58401..59630
                     /locus_tag="PK049_01395"
     CDS             58401..59630
                     /locus_tag="PK049_01395"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HOK60239.1"
                     /translation="MKKIVILGAGTGGTIMANKLRKVLERDEWSITVVDKDPNHYYQP
                     GFLFIPFGIYSKHDVVKPKASFIPAGVKFIVNDIDRVEPDKNTVYLTNGQILEYDFLI
                     VATGTHINPAETPGLLGREWRKSIFDFYTHEGAVALSTFLKTWEGGDMVINIAELPFK
                     CPVAPLEFAFLADEFFVKKGIRDKVNIYYTTPLSGAFTKPKSSKMLGELLKEKNIQII
                     PDFYLERVDDENKKIISYDGREVKFDLLTIIPVNMGADWVARSGMGDDMNYIATDKYT
                     LRSEKWENIFVLGDASNIPTSKAGSVVHFASDVLFENLMAAIEGRPLIAKFDGHSNCY
                     IETGFGKGSLIDFNYETEPLPGTYPLPGIGPFGLLKNTRINHYGKVMFRWIYWHLLLK
                     GKELPLESQMTMAGKEL"
     gene            59643..60317
                     /locus_tag="PK049_01400"
     CDS             59643..60317
                     /locus_tag="PK049_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60240.1"
                     /translation="MENNLQQQIDDLNRKMDLVLELLAKQQQRSEVVEDLVEDLNIVV
                     KDAFQTAVDELAVQNIKIDGEDVRYLVFKLLRNVKTFGELMDMLESLMDFLHDIGPVV
                     HDAGIAFTKALDDLDRKGYFDYFRQLGLLAAEFKEHVTEEDIIRLRESMPAVGLMLRS
                     LTQPKVMNALAEVTQTLSSMEMDEKIDNKSLIGIARQLSKPEVRRTLSFMLRVMGELG
                     KTKKIN"
     gene            60339..60650
                     /locus_tag="PK049_01405"
     CDS             60339..60650
                     /locus_tag="PK049_01405"
                     /inference="COORDINATES: protein motif:HMM:TIGR03342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HOK60241.1"
                     /translation="MAEKVYAGKSVSVTDDGYLTDPNQWTKEVAIEIAKEEGIELTDK
                     HFAVIEFIREKTLKGEALTIRTIGKSGLVDTKGFYELFPGAPLKKASRIAGIAKPSSC
                     V"
     gene            60669..61172
                     /locus_tag="PK049_01410"
     CDS             60669..61172
                     /locus_tag="PK049_01410"
                     /inference="COORDINATES: protein motif:HMM:NF025066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HOK60242.1"
                     /translation="MANAQDHPIKKVMIICAKANLEDVYAALVMANGAVMEGIEAKVF
                     FTFFGLDAITKKQMKRLHTATVGNPGLRMPGGLPFPTLLGMLPGVEAGVSAMMKSQME
                     KLDIPPVDEFLELITAGGGEIYACKLAVEMFGLKKDDFSEHVKDIITIGQFYELAAGE
                     GTQIIFV"
     gene            complement(61225..65460)
                     /locus_tag="PK049_01415"
     CDS             complement(61225..65460)
                     /locus_tag="PK049_01415"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOK60243.1"
                     /translation="MTLGRNFLLALFFTLFQFKTLYSQSEFSKVETDYYTSFINYYQS
                     DGICSYEFTDIIQDGMGLMWFATQDGLMRFDGNNFKTYRGGKTKGQLPHSVVSSLAVD
                     KKKNLWVGTKGGLCVYHPESDTFERVDSLFGEPLQGHRWVRDIYVDSKGRLWVDRHNG
                     TLNCIDFDAKTVETFQHEYVYNEIYYFHAITELGEGVMLGGGSAHLTWLTRNPDKWNT
                     YPSVETIINNEIITIAGTANFYDDGSDYIWVANYVDNAYRINKKTMERTPLPLPSVYT
                     ICPNGDGKVWMGGYHFGVVLYNPSTNTATRYIRMEGNPQSLAGNMILVIYRDNQGNTW
                     FGTNNGLSLLTTFARKSTHLRKIADVNSLPSNQITCMFPDYPKGLWLGTQNKGLVFID
                     FNTKNVTRFGYFNHPDSLASNWVTSIARTTDNSMLVTLWNGYGGALNRFWPEKKRFKR
                     YNPDGYYWYSNVITTSDGKALFSPWGGRVNEFDPRTENVTSRIFGCFENQCSNVSGYI
                     NKITTSNNGWILFEFATLLNVLNSDTLNIVPSNFTNLDIGIPKQKQVTFNIQAKKMLL
                     FNSNLMILGNDNKIYPFSLPTRKIEPPLSTNINFTNIFVDKYLYALTDSVLFRWNQER
                     GRFDQLTSIKQFPLIKSIADGNDTIFLGTSEGVYVMTEHTKTLTKLFDADVQALTFYK
                     GFAFGASNKGLLMFNGKGLVKRYLSEQSITNFTIDSSGNVWATNSEELFRLSLKTNSI
                     ETIGAYPQNKDSLPSVQVKSIATDALGNVWILSDVCISKYLPKQKRYESYYVPGKSAL
                     QSTLVYRMYEDSKGYVWVGYTRNGVGVDRIDRRTHTVDHFPYLPYDSTSYPATSMTHC
                     IFEDANGNMLFGTDAGLAILQPESKAFQMIDTRHGLPSSVVTAIYQDRLGNYWIGTDK
                     GIARFSKKLKRVDITGKNLNFSEGLVTAIEQWGFDSLVVSGNFGLYIFNPYVQGNEVP
                     MQDIKILSCETDYDTLARFPANNSKIFVKANHKWLKIHFTASDYTSPGALEYTYWLQG
                     FEDSTRAKKTHSPLAEYTNLPYGKYTFKVYYTRPDGTKSKNVFTINVVVGMPLYLKPW
                     FFLIFLLLLALSVYIHEILRVQRVKRKKDELEQAVMEKTKAILAQNEELKAQADLIKQ
                     QSNNLKLKTYQINESLEFSKLLQSNLMPSPEILSTILGEHLIFYKPKDVVSGDFYWLK
                     GNSDEFIIAVADCTGHGVHGGFVSMMDITLLNEVYTHYKINNPVELIIETHNHFNQTI
                     GAQLQLNASSAPVEMDIAVCYVNRINKTALFASTHNPLYHIQRNFESPELKIYKSGGK
                     AVGNRWFTPNIPLVELKLESGDILVLTTDGLFDQLSKSEKKRFGRKQFESILLENANG
                     NLDTINKKIEQCFDDWRGDSQQTDDITIIGFRI"
     gene            complement(65465..66625)
                     /locus_tag="PK049_01420"
     CDS             complement(65465..66625)
                     /locus_tag="PK049_01420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HOK60244.1"
                     /translation="MISKSDTENILVEYRRHLHKHPELSFQEVKTSSYIKQIFATEGV
                     RVEDFGTHHSFAAIVEGKLPGKTYGLRAELDALPITETSGVAFASVNPNVMHACGHDM
                     HMAIAIGVTLSLYRQRDKLKGRFVAIFESGEEVLPGGAQAIIQTKLYNELKPDAMFGV
                     HVLPELEVGKIGVCPGKYMASGDEVYITVKGKGGHAALPHLLIDPITCAAEIVLGLQT
                     LVSRKAPTLIPTVLSFGRIEANGATNIIPDNVSISGTFRTMDEQWREEAHKLIQQTAT
                     GIAASHGAKCEVEIRKGYPSIFNNPELTQLVKQTATELLGNDKVVDLEPRMTTDDFAY
                     FSTLTPSVFIRLGAGFPGSDNPQLHRPNFNPSEGAIGIAHSLLTRILLTLMK"
     gene            complement(66622..66990)
                     /locus_tag="PK049_01425"
     CDS             complement(66622..66990)
                     /locus_tag="PK049_01425"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOK60245.1"
                     /translation="MSIAHLVLLLLIGLTAGFVGGALGLGGGIIIVPALIYLLNFSQH
                     EAQGTSLMVLLFPVGILAVVNYYRAGFIDWKLALILMVGVVIGSYFGSKVSINIPAET
                     LRKIFGFFIVLVGIKMILGR"
     gene            67129..68220
                     /locus_tag="PK049_01430"
     CDS             67129..68220
                     /locus_tag="PK049_01430"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOK60246.1"
                     /translation="MKKILTISVLAAFVFSVAAQNAVPKINFKTSTYDYGKIKEDDGV
                     ANYSFEFTNTGNAPLIIQRVATSCGCTTPEWPKEPIAPGKGGKITVGYNPAGRPGPFA
                     KTVTIYSNAETPTVILQIKGEVIPHVKTIDEIYVTPIGDELKFEKIHFALGRIFLGST
                     YNDTIRFINKSSAPVTVKANTQGMNFMQVKVVPEVVKPNQFGAIVLTYNPDKREEWGF
                     VVDRFTISVNDKAMNTSPITITASIEEDFSKLTQKDLENAPKAEFNTLQYDFGTVKSG
                     DPVNYDFVLKNTGKTDLIIRKIKASCGCTTATPSKSVLKPGESTEISASFRTAGYSGR
                     QGKTITVITNDPRNSTLILRLTGNVDKIE"
BASE COUNT        19474 a        14437 c        15228 g        18946 t
ORIGIN      
        1 attcttcttc gccacgacgt agacctaaaa ccccaaaatt cgcttgcaac agcaataata
       61 gaaagaaaac ttggaattca aggaacgtat tatttccgtg tggttcctga aagctataac
      121 gaggatatta ttaaacagat tgctgatttg ggccatgaaa ttggctacca ctatgagaat
      181 atggattcgg ttattacaag atttagaggt aagggtatta agtttaaaaa tgctgaagag
      241 gagcgtgaaa gtattattga tgcagcatac gaagagttct gtgagaatct agaacggttt
      301 agaaaaattt atcctgtaaa aaccatttgt atgcatggta gtccaaggtc aaagtgggat
      361 aataaggaaa tatggaaaaa gtatgattac cggaaattgg gcatcattgg agagccatac
      421 atcgatatag attttaatga agtgttctac ctaaccgata caggcaggcg ctgggatggt
      481 ttcaggtact cggttcgcga tagggttccg cagcaacaaa aatggattga gcagggtctg
      541 gttttcagaa ccaccaacga tattattaag gctgtaaacg agaatcggtt tccaagtaaa
      601 gccatgataa ccattcaccc ccagcgctgg accgacaact accttgaatg gactaaggaa
      661 ctagttacac aaagagcaaa aaatatagtt aagtggggat taatgcgttt gagagatagg
      721 taatggaacg cagatgacac cgataatgca ggttttcact gtgtaacacc gtgtaaactc
      781 tgtgaaaatc agtgatactg atttggttga tagttgacag ttgttggttg ttagtattag
      841 aactctaatc cttgaaccct gaacctttat tcccgcatcg cgggacaggc tcttgaaccc
      901 taaactttgc actttgaacc ttgaaccttt atccctgaac ccgttcctgc acactaacta
      961 ctacattcct ctccccacac cacacgtttc acataatcgg tatagcttag gataatccga
     1021 accaccttat cggatacgtt gggcatggag taatcggcaa ccgggcggaa gtttcgctca
     1081 gtgccgattt tttgttgctg tagctgagcc aatccctgaa tgatgcggtc gggattaagg
     1141 cctaccatca ttactgaggc ctcctccatg gcctcaggcc gctcatgcgc ctcacggata
     1201 ttcagcgccc taaagttgag tatgctcgat tcctccgaga tggtgccact atccgatagc
     1261 acggcgtagg cattcagctg aagggcgtta tagtcggtaa atccgagcgg tttaaggaag
     1321 tttaccagcg ggtttacctg cactcctttt gcctcaatca tcttgcgggt gcgcggatgc
     1381 gtgctaacaa taatggggta accgtaggta ttggctatca ggttgaggga gtggataagg
     1441 tttgtgaagt tccgctcgga gttaatattc tcctcgcggt gagccgatat cacaaagtac
     1501 tcgccccgtt tcaggttcag gcggctaaga acatccgatt gctgaatacg gggtaggtag
     1561 tggttcagca cctcaaacat gggcgaaccg gttttaatca ccctatcggc agggagccct
     1621 tcgcgtagca ggtactcgcg ggcaatgtcg ctgtaggtga ggttgatatc ggcaatgtgg
     1681 tcaacaatct tgcggttggt ctcctcgggc acccgctggt cgaagcaacg gttaccagcc
     1741 tccatgtgga atatggggat gtgccgtttt ttggcaggga tggcgcacag gcagctgttg
     1801 gtatcgccca gcaccagcat cgcatcgggc ttgagggtct cgagcagggg gtcaatctta
     1861 atgagtatct ggccaatggt ggccgtggcg ctatcgccgg cggcatcgag gtagtggtcg
     1921 ggtgggcgaa tgcccatgtc gtcgtagaat atctggttta gctcgtagtc gtagttctgc
     1981 ccggtatgga cagttatatg ttcaatggca gggctctggt cgagcttggc cattacacgc
     2041 gacaggcgga taatctcggg acgcgtacca acaacggtaa ggactttaag tttcttcata
     2101 gttctggtta cacagtgttt cacaatgttt tcacagtgtt acacagtgca ttactctgtg
     2161 atactctgtg aaacggctat tcattaaaca gtttcaaaaa acgtatccgt atcgctaggg
     2221 tcgaagtgct cactgatcca gaaaatggtg taaacatcat cgttccccac gtttttgatg
     2281 ttatgggtaa accaaatggg catatccacc accgatgggt ttctatcgcc atcgagctca
     2341 aaggtaagct ttttatcggt tccaatgcgc cgtaactcaa ttactgcttt gccctttatc
     2401 acggcaaacc tttctgcctt gcgggtatgg aagtggttgc cgcgggtaac gccgggcttg
     2461 gtggtggaga acgaaacctg tccgccgctg ttcagcttca cggtctccac aaatgagccc
     2521 ctgtcgtcgg ttttcagctc aagcatacgg gggaagaact tttcgtggtc gatgtagcaa
     2581 acaaaggtgt tgaataggtt gcgttcaaag ggatcggtta ggttggggaa aataccctta
     2641 tcgaagtagt tggatttgaa atcgtttagc cttgccaaaa gggctgaaac cttgatggtt
     2701 gctgtatgtg ggatttgaac cgtttcaacg tgggacactg catttctgct ttgggctacg
     2761 ctatcgattt tggtaataaa atgctctacc agctccgaaa cgtaaatcaa ccttaactcg
     2821 ccatccacat caattttggg ctcctcgtta tgcgtaagct ggtggcagaa tgttgccacc
     2881 acggagttgt agtagggttt gccaaagggt ccaaacacgt tgggtatgat aaagccgctg
     2941 aaggcagtat ggtttcgcct ggcccactga tcaaaaagca atcgcccttc gcgcttcgat
     3001 ttaccgtaga ggttatcgcg ctcctcctgg gttgatgagg agaagatgat gtggggtttt
     3061 gagcgggttg cctcgcaggc gttaatgagc ttttggcaaa ggttgatgtt ggtttggtaa
     3121 atgacctggg ggtcgttgtg gcggttcatg gcggccaggt gtacaatggc atcgcacgac
     3181 tgaacaaagc cgttgagctt aatgggatcg tcgaagtagc tatcctcaaa ggcgatgcgc
     3241 tggaacctgt cggggtatag tcccagggtg ttgtacaggt gtgtgccaat aaaacctgac
     3301 tgtccggtaa tgcctacttt gatcatgatg ttttttaata gttaagttcc acagtgttcc
     3361 acagtgttag cacagagtta cacggtgttt attacaaaac taatcgctta attccatctt
     3421 ttagactttt tgtgttaaaa ttaatcagaa gtccaatgtg tttattagca agtttaaggt
     3481 aggttaaaac ctgtgcaata tgtacatcag taaatgcatc tacgcttttt agttctacaa
     3541 caacctggtt ctcgaccagc aagtcgagtc gatacccaca ctcaagctta atctcctcgt
     3601 aaattaaggg aacaggtttt tctatctcaa cctgaaatcc ttttttctgt agttcataag
     3661 cgaggcaagc ctgataggta gattcaagaa ggccaggacc taaagtagaa tgcaccttgt
     3721 aggctgaatc gattatttcg tgtgtaatct ggttgatttc catttttaca ctgtgctaca
     3781 ctgtgttaaa ctcagtgaaa ccctgtgcta ctaattatat ttaaggttcg tatatatgcc
     3841 cattgtcctc gttcagtata tcctttcgga taaggggtag ttttagcagc agttccttca
     3901 ttccctccac atccagcctt cgggtgttgt gcgagttgta ctcctcaatc tgtgtgatat
     3961 cggtttgtcc ctcgctgaag tacagaccat agttcaggtc gcggttatcg gcggggatgc
     4021 ggaagtactt gcccatgtcg taagccttta ccatctcctc gcgggtgagc agggtttcgt
     4081 aaagcttttc gccgtggcgg gttccaatta ccttaatggg attcttggca tggtaaagct
     4141 ccagcagagc ctttgccagg gtctcgattg tagccgcggg tgccttttgc acaaagatat
     4201 cgccgggctg gccatgctca aaggcaaaca gtaccaggtc aacggcatcg tcgagggtca
     4261 tcatgtagcg ggtcatgttg gggtcggtaa tggtgagcgg ttgcccggcc ttgatttgct
     4321 ccacaaagag tgggatcacc gacccgcgcg aggccattac attgccgtaa cgtgttccgc
     4381 aaaaaacctg tttgcttggg tcgttattcc tggaacgggc aatcataatc ttttccatta
     4441 gggccttggt aatgcccatg gcatttatgg ggtaaacggc cttatcggtg ctaagggtaa
     4501 ccacccgttt aacatggtac ttttcggcag catcgagcac gttgtcggtg ccaagcgcat
     4561 tggttttaag ggcttccacc gggaagaact cgcacgatgg aacctgctta agcgcggcag
     4621 catggaacac aaaatcgacc ccaaccatgg ctgaacgaac acttccctcg tttcgaacat
     4681 ctcctatgta aaacttgata cgcttatcgt tgtaaaaacg gcgcatatcg tcctgctttt
     4741 tctcgtcgcg cgagaaaata cggatttcgg aaatatttgt tttaaggaat cgcctcagca
     4801 ccgcgttacc aaacgatccg ctaccaccgg taattagcaa cgatttgtta tcgaaaatac
     4861 ttttgggcat atcggtaggg tttattcatt caaagttaac aattttagtt gatagttaat
     4921 ggtgaattta attgaacaca gatgacgcag ataagctagt tgttggttga tagttgatgg
     4981 ttgtaagtga attggaatgg aacacagatg gcgcagactg ggcggattta cactgtgaaa
     5041 cactgtgtaa accctgtaaa acactgtgat acttatttgg ttgaaagtat tacgaaaaac
     5101 aaaaacgtcg ggctttggct ccgacgtttt ttaatgtttc tacttattcc cttttttagg
     5161 ctttacgttg tcctgtggga taaaaaccga aggtttaagg tatgagtaaa tcaaaaatcc
     5221 aattcctgct gcaataaagg gtaagcttag ccactgcccc atgtttagca tcatgtgttt
     5281 ttcaaactca acctgtggtt cttttacgta ctcaattagg aatcgtgcgc caaagagcag
     5341 gacgaggaag atgccaaaaa tgaaaccatt acgcagcttg ccatcggcct ttaggtaaag
     5401 ccagtaaagc aggataaata ttgcggtgta tgatagcgcc tcgtaaagct gtgtggggtg
     5461 ttttgggtgt acctcgccat tccgcacaaa aataaaaccc cagggcagtg tggtttcaac
     5521 cccgtagatt tcagaattaa acaggtttcc taggcgaata aaggctccac caattgctac
     5581 ggttattaca accctatcaa taacgtaaat catggggacc ttatgcttgc gggcaaaaag
     5641 gtaaagggca attagtatcc ctatggccgc gccatggctg gccaagcctc cgtgccagat
     5701 cttaaggatt tcgagcgggt tgctgaggta gaattccggt tcgtagaaaa ggcaatggcc
     5761 caagcgggct ccaattactg tgcctacaat catgtacatg gttagcgagt cgaggaaacc
     5821 ctcgggcagg tgctcacgct taacaaagcg gttaaaaatg acatagccaa tgtaaaaggc
     5881 catggcccac attaggccgt agtatcgtac tgagaacggg ccaatactaa atatctcagg
     5941 attgacattc caggttatgt atgcaagcat agcttaaaat ttttgtaaat gtaagccaaa
     6001 aattgttttt gaagaaaatt tgtcccttat attgatgtat agtatgggtt aacaggttta
     6061 gttgtaaggt gatggttgat ggttgatggt tgacagttga tggttgttag ttaaggtgtg
     6121 tttagtgttt tgtgtatagt gtttagtttg gtaatggaac gcagacgaca cagattaggc
     6181 cgattttcac tgtgaaacac cttgaaaact ctgagtaacc ctgtgatacc gatttagttg
     6241 atggttgatg gcataagagt ttacctaaaa tcttgaatct gaaatctgaa attttaaatc
     6301 aatgttgttt agttaaggtt attagtcatg ttgcgcgcga gcgaaacatc acgctgtcgt
     6361 gctgagttcc aatttatagg gatagacttc gccgaagcat ctcataatac cttcatccat
     6421 tatttaatga gattcctcgg ctacgctcgg gaagacaaaa acccaatctt ctcttctact
     6481 cctttgtcat gctgagcttg tcgaagcatt gctttgaacc ctcattccct tttaaatgag
     6541 attcctcgac tacgctcggg atgacaacat gatgggctcc ctcgctcact ttgtcatgct
     6601 gagcccagcc gaagcatctc tttgtacctt catgccctaa atgcctttta ttgttcttcg
     6661 tcatactctt cagttgcgat gcaaagcttc accgttgctc tttggaaaaa tattgcgtgc
     6721 atcgcaatac acatgggagt agccatggag ttacaaatgt tacgccccta cggggctgga
     6781 aaggttgttc gtggaattga cctcacccca gcccctctcc tgacaggaga ggggagatgc
     6841 aataaggaaa ccttattgca gggctaaagt ctcccctctc aggggagatt tagaggggtc
     6901 atctaggttg aatgtggaaa acggcctcac cccagcactt taccctgaga aggaatggag
     6961 cggaaaaagc aagcagaggc ataaaagccc gacccggtaa tgggtcgggc ttttatgcct
     7021 tgattttctt tggttctttc ttgtatcaag acaagaaaga acatgtaaaa ggctctttgc
     7081 aatgatttac ttttgcgatg caacgtttca gttttattct ttggagtatc attgcgtgca
     7141 tcgcaataca taggggagtg gccatggagt tacaaatatt ccgcccctac ggggctggga
     7201 gcgttgttcg ttaattccat tctgtgtaac accgtgttac actgtgtaac cttgtgatac
     7261 ctatttagtt gatggttgtt ggttgacagt tgatagtaaa ttcaatagaa cacagatagc
     7321 acagattggg gtgatttacg cagattttca ctaaacacga aacaccaaaa aacgaaacac
     7381 taccccgctg tacactgcat actgaacact gcacactaat aatgatatcc tttgctccca
     7441 gctatggcgg gacaggacgc tcaggatgac aggtagcgtt gctctaatga aattaaatac
     7501 tacagtgaac cccctttaca ggttaaactc cttgcggaga atatcaacat agtcgagctt
     7561 ttcccaggta aagaattcca cctcgcgttt aaccaccttt tcgccctcac agttgccatt
     7621 cacaataaat tccacctcgc gcttgtagta atcgcgacca aaatggccgt aggcagcggt
     7681 ttcctcaaaa atgggatttt taaggcccaa acgtttaaca atggcacccg gacgcaaatc
     7741 gaagatctgc ttaatttttt cagcaatctg gccatcggta agcttaacgt gcgaagttcc
     7801 ataggtatta acgtaaagcc ctacgggctc ggcaacgcca atggcgtatg ctacctgaat
     7861 gagaacctca tcggcaatgc cggcagcaac catgtttttg gcaatatggc gggctgcgta
     7921 tgcaccggag cggtcaacct tgctggaatc cttgcccgaa aaagcgcctc caccgtgggc
     7981 ccctttaccg ccataggtat caacaataat tttacgtccg gttaagccgg tatcgccatg
     8041 cggaccgcct attacgaact taccggttgg gttaacgtgt aaaatgtaat cgtccttgaa
     8101 cagcttttga actctttcag ggaaacgggc tttagccctt ggaataagga tagtgcgaac
     8161 atcctccttg atcttgttca gcatccgaac atcgtcggta tcaaactcat cgtgctgggt
     8221 ggatacaaca atggtatgga ttcggagagg ctggttatta tcgtcgtact caatggttac
     8281 ctgcgacttg gcatcgggac ggaggtaggc catctgctta ccctccctgc ggattttagc
     8341 aagctccatg agcaacacgt gcgaaagcac aagggcaagt ggcatatagt cgtcggtttc
     8401 gcggcaggca tagccaaaca tcataccctg atcgcccgca ccctgtgaga actcatcgcc
     8461 acgttcaaca ccacggttaa tatcgggcga ttgttcatgg attgatgaga taacgccgca
     8521 cgactcggca tcgaacctca gctcgtgctt ggtgtaacca atccggcgga taacccggcg
     8581 ggcaatctcc tgaacatcaa catatgcatg ggttttaacc tcgccactta acacaaccaa
     8641 accggtagta accagtgttt cgcatgccac ctttgagttg gggtcgcgac gtaaaaactc
     8701 atcgagcaga gcatcggaaa tttgatcggc cagcttatcg ggatgccctt ccgatacgct
     8761 ctccgaagta aataaatatc ccatagtaaa aaacttttag gttaaacaaa tatctatggg
     8821 gaaggtttga tcgattgctg gaataatcgc tgttttagca tttatttttg tggttgcaat
     8881 caaccaaatc tttccacatt cagctcacaa atgtatactt ttaacgtgta tgtaacaact
     8941 ttcaatgctg taaattggaa ttttctacgc caatggcttg cgctgcttaa tgtagaggta
     9001 aaccatcgat acaatacctg caataaccac cattatggtt tgcgattcgt gcgagatgat
     9061 tgcaaatatc aacccgttct cgtaagtgat tccaaaaatg ccaagggccg aggcagtaat
     9121 aatgtggtaa gccccaaaac cgccctgtac tggaacagtc atgccatagg aaccaataac
     9181 catgataaac agggcatccc agccagtaag gttcgatgta attggggtag agcaaagtaa
     9241 agcccaggtc attagccagt agcttaccca aattaaaacc gtgagcagtg cgaattggat
     9301 taccctgggt gttcggtaaa tactctttag tccatcgata acacctatta cagcactttt
     9361 gattttcccg ttcagcttct ggcccaggaa atcgcttttt aaaatgaaac ggataaggag
     9421 taaaaatgcg attgccatca ccagtagaat aacaatcttg tatccctcaa tatgtatggc
     9481 cttttgctca atgggatgaa caatttttgt gtagatatac tggccaaagg aatcgatttt
     9541 gacaagaacc acaataacag ttgcaatcag gagcattgca aggtcggcag cgcgctcaac
     9601 cacaacggtt ccaacaagcg actcaaaacg gattcgggaa accctactta cgctaccaca
     9661 ttttgccacc tcgccaaagc gggggagggc taagttagcc aggtaaccga acattactgc
     9721 tgcaaacaag ttgctgaatg gaggtttgtg gccaagggat tccataaggt aaccccagcg
     9781 aatagcccta agagcatggg caataagggc aattaaaagc gaaatggcta tccagaaata
     9841 gtttacctcc ctaaaaccct gagcaatatg ggtaaaatcg acactcctga aagcgtagta
     9901 gagcagtaca atggcaagag aaaagaatac ggcaaagtta aatagccttg atttcactat
     9961 gcttttcaat gcagtaaacg ggtttgttat acaagcttgt tggtggcggt gtcggggaaa
    10021 attacggtgg gcttaaactt tttggcctcc tcaaaatcca tggtagcata ggaaatgata
    10081 attaccacat cgcccaccac gcacttgcgt gcagcaggtc cattgaggca aacctgacca
    10141 ctaccacgct caccctttat cacgtaggtt tcaaggcgtt cgccgttgtt tatattgaca
    10201 atctgaacct tttcgttttc aataatgttt gctgcatcca tcaggtcctc gtcaatggta
    10261 atgctaccaa tgtagttaag gttagcctcg gtgatagtaa ccctgtggat tttcgacttg
    10321 caaacttcaa tcatcatcgc cttatactgt tttcggaact atttaaacat catgttatcg
    10381 ataagcctta tattaccagc ccaaaccgca atgcaaccaa caattccgtt ggggtgatcc
    10441 cagccctgaa caggcagcag ggaatcggca tcggccagct caaagtactc aacctttaga
    10501 tttgggtcag agttgattct atttacaacc caatcaatca tatctttaac ggaaaactca
    10561 ttggttttgt ttcgtgcttc agctaatgtt tggtaaatga gcggggcgct tttcctatgc
    10621 tcaggggtaa gcagcaggtt acggctgctc atggccaacc catcgggttc acgcacaata
    10681 gggcagccaa ctatttcaat ccggtagccc agcatacgaa ccattgccct aataatggcc
    10741 agctgctggt aatccttttg gccaaagtat gcccggtgag gttcaacaat atcgaaaagc
    10801 ttgcttacca cctgggcaac gccgttgaag tgtcccggac ggaacttccc ctccatcacc
    10861 ttgtcgagca tcccaaaatc gaacacacgg gtatcgggct caggataaat ctcgctctcg
    10921 gtgggcatga aaactacatt aacccctgcg gcctccagca tggcaatgtc cttttcgggc
    10981 attcggggat agttctttag gtcgttttta tcgttgaatt gtgtgggatt tacaaaaatg
    11041 ctcacaaccg tgatatcgtt ctcagcaaca ctgcgctcaa ccagcgagac atgaccttta
    11101 tgcaatgctc ccatggttgg aacaaagcca atgcttttgc cctcgctcct gaagccctga
    11161 agcatcgttt ttagatccga tatcttatca acaaccacac acataggtaa ttatagttta
    11221 ctgaggtatc gactttgaac ttgcaaaggt tgtaaatttt tgaataacat gtatgttgat
    11281 ttttactgtt gatgccctaa aacatatcaa aaagctatat aattaacttt aaatggacac
    11341 cgataacaac ttaaatttga catgtatact ggtattcaat tacataacaa attaatcaat
    11401 atcaagaaaa aagttgtaac tttgtagcct attataaaaa tgcgccttgg tgattgatgt
    11461 tttagctatt taatcaaatt cggcaaaagg ataatccaaa atgaaagaaa aaaaggcgaa
    11521 gatactcttt atctctcagg agatacttcc atacctgcct gagagtgagg tttcaaaaat
    11581 cagtaggttt ttacctcaag ggatacagga aaagggtcga gagataagaa catttatgcc
    11641 aaggtatgga tgtgtgaatg aacgccgaaa ccagctacac gaggtgatac gcctttcggg
    11701 gatgaaccta atcatcaacg acatggatca cccccttatc ataaaagtgg catccattca
    11761 aagtgcacgt atgcaggtgt acttcattga taacgaggac tacttccaaa ggaaatccac
    11821 cttaaccgat gaaaacggcg aaaccttcga cgataacgac gaaagggcta tctttttcag
    11881 caagggagta attgaaaccg ttaaaaagct acgctggtcg cccgatttaa tccactgcca
    11941 tggctggttc accgcttttg ccccactgat gatcaaaaag gcctacgccg acgaccctct
    12001 gtttgcccac tctaaggttg ttttctctgt gtataacgat ggttttagca aacccttgaa
    12061 cgagaatgcc aggaaaaaag tgttaatgga gggtatatct aagaaagatg ttgagatttt
    12121 acgagaacca acctacgaaa accttctgaa gcttgccgta aacttttccg atggggttat
    12181 tttgggtagc cctactgtta acggtcaggt gcttgaacat ataaatacag ctaaaaaacc
    12241 tttactcccc taccaggata gtgaaaatta tattgatatt tacgacgaat tttataacca
    12301 gatactaaat ggcaattcaa atgtttcaaa aaagaagtag tattggaatc aggcatttgg
    12361 gtgttttaat ttcaggcctt tttctttcga tattttcgct cacgtcatgc tataatgaac
    12421 ccggtttttt aggcaacaac cttttacccg acgaggatat ttacaaggta aagatagatt
    12481 cctcgttcaa ggttagcgct tttaccctta caaaggattc gctaaactca ttccttgcct
    12541 ccgagggagt aatggggtat gttaacagcg agatttttgg atcgaccaag gctagctttg
    12601 ttggccgcta cctcccggga aaatcaaccg atggttacgg cgggcctact gcaactgccg
    12661 attcaatatt tttctacttt accgcaagcg gtttttacgg cgattccacc actgaactta
    12721 acctgaggat acacgagatt actgatacct cgctattctg ggacaagcag aacgccctca
    12781 gccctattaa tgttaataca tacaacccaa ccccttttgt taccgcaacc ctcaaaggaa
    12841 aaggaagttt gaaactacca ctcccacttg cgtttggtca atcgttaatg gactcgctgg
    12901 cgttaaccga ttcaaaaata ttttacacca agtttaaagg gttctatgta accttcgacg
    12961 agctacccgg ttatggcggt gtagcctata acgtatcaac ctcaaacatg taccttaggc
    13021 tatactacca ctacgttaaa cagattgacg gcaaggactc aaccattaaa accagcaaga
    13081 cctatacttt tggttcaagg ttttacgagt acctgcatga ttacacaaag gccgatcctg
    13141 ccaaaaaaat acaacacctt aacgatacca ttactcagga tacagttttc tatacccaga
    13201 gccttggcgg ggtttatggg aaaataactt tcccggacct gacacaatgg cgcgactcca
    13261 tgcccattgt aatacatcgt gccgagctca taattggtaa ggccgatgcc accacatcgc
    13321 taccagactc aaccattagt cagctcctat tttactacaa ggatggtaac gagtggattg
    13381 gactgatcaa ggatcagata actagcacaa atacccttag ctcaaacggc tcgtaccgga
    13441 cattcaggaa tggttacagt gtcaatataa cctaccattt ccaaagaatg ctaaagggtg
    13501 agtatagcga caatagcctg tatgtattcc caaactcgaa tactaccatt aggcatgggg
    13561 tgcttaaaag cggacagaac agtaccccaa ttaaactaaa aattacatac accaagctca
    13621 aatagtatgt gcggaattgt tggatatatt gggtataggc aagcttttcc tattgtgata
    13681 aacggtttaa aacgcctgga gtaccggggt tacgattcgg ccggtatttg tatagtaaat
    13741 ggcgatccgt ttcttgcaaa acagaaggga aaggtaagcg atctggaagc ctttattggc
    13801 gatagaccgc tacccggcac aattgggata ggccataccc gctgggctac ccacggcgag
    13861 cctaacgaca taaatgccca cccccatgag tcgatgaacg gcaagttcat ccttatccat
    13921 aatgggatta ttgaaaacta cagcctactc cgtagcaagc tggagaagag gggttacatc
    13981 tttaaatcgg aaaccgatac tgaggttctg gttaacctga ttgagtacat ctacctgaaa
    14041 ggaaatgtta cccccgaaat tgcagtccgc ttagcgctga caaaggttgt aggagcgtac
    14101 ggtatagttg taattaaccg cgatgaaccc gatacgctta ttgcagcccg caagggcagc
    14161 ccgctggtaa taggggttgg cgaaggcgag tactttattg cttccgatgc cactccaatt
    14221 gttgagttta ccaaaagtgt aattttcctt aacgataacg atgttgctgt actccagcgc
    14281 aaccaaatgg ttctgaaaac catttacaac caagcgcttg aacccaacat ccagcagatc
    14341 gatattgata ttgaggcaat agagaaaggc ggttacgagc acttcatgct caaagagata
    14401 ttcgagcaac cgcgtagcat acacgacacc tatcgtggcc gtatctcgag cgacccattt
    14461 gaaattcacc ttggcggcct gtatagcgta ctaccaaacc ttacagaagc caaacgtata
    14521 attctaatag gctgtggtac atcgtggcac gctgcgcttg tgggtgagta cctgattgag
    14581 gatttggccc gcatacccgt agaggttgag tacgcctcgg agttccgcta ccgcaacccc
    14641 attatcaata aggatgatgt ggtgatagcc attagccaga gcggcgaaac cgccgatacc
    14701 ctggcagcaa taagacttgc caaggaagcc ggagcgcttg cgctaggaat ttgtaatgtg
    14761 gttggatcaa gtatcccccg cgaaacacac gccggcgttt atacccatgc aggccctgaa
    14821 attggggttg catctaccaa agcatttacc gctcaggtaa ccgttctaat catgatggcc
    14881 atactgcttg gccgcaggcg tggtacactt accgaggagc gttaccgcga acttattcgc
    14941 gaggttaccc ttgtaccgga taaaattgag aaaatactac aaagcgagga gctagtcaaa
    15001 gttatttcag agcgttttaa ggattcacgt aacttccttt accttggtag gggctactat
    15061 ttcccggttg cgcttgaggg cgcacttaaa ctcaaggaga tatcgtatat ccacgctgaa
    15121 ggttatcccg ctgccgagat gaaacatggg cccattgccc tgattgatga taccatgccg
    15181 gtagtggtta ttgcaattaa ggacagcagc tacgagaaaa ttgtaagcaa cgttcaggag
    15241 gtgaaagcac gcaagggtgt tgtaattgcc gttgtaaccg aaggcgatac caccattaag
    15301 gaaatggccg actataccat tgagataccc gaagcaggcg agattgttgg tgctttgctt
    15361 accgttatcc cattacagct actggcttac cacatagccg ttatgcgtgg atgcaacgtt
    15421 gatcagccac gaaacttagc aaaatcggtt acagtggagt aaatcaagca tattctataa
    15481 ataaaaaaag cgcgtttaac gcgctttttt tatttatact tagcaagttt ataccagtaa
    15541 tcggcctttt gcttattctc aaaacgggta tagatgttac ccaggtaacg ggcatacctt
    15601 ttatcgggct tcagcctgta aagggtttca aagtaatcgc gcgagatttt ataatcggca
    15661 ttcaccttct ttaaagcctc ggtaagctgc ttatactgtt tggtggtctt gttcttctcg
    15721 taagcgttca tttcgctcac gtaaagctca tcggcccggt taaagtgata ctctccaagg
    15781 aattccagag cttcggtatt atttttatcg tgctttagta actcctttgc aagcatttcc
    15841 gctttctggt tgtttttcag ctttcggtta acaatcaggt agcctgtttt aaggtttaca
    15901 tcggactgat actgtggggc taatgcattc cataatgaat cggccaagtt ccagttctcg
    15961 ctccttatgt atgcatcaaa aagcgtagcc cttgcctgag atagcaattc cggttggtta
    16021 aggtcaacgc aaagtttaag gttaacaatc tcacgcgaaa gattatcaat tttacggtag
    16081 gcctttgcaa gtgcaaataa gccctgagca tcggcactgt tttccttctt ggctttttcc
    16141 aggtaatcaa ttgccttttc ggtttgtcca acctcccatg cggcaatacc ggctttgttc
    16201 caaacgtcac cggtaacacc tttacctgtc gcaattagct gttcatacga agccaatgca
    16261 gcctgatagt cgttcatttc aaaagctcgc ttggcctcat cggatagctg cttatttttt
    16321 ttcgaaaccg cacaagaaag tactagcgtt cctattagga caaatgttag cgggaatttt
    16381 agttgtttta tcattatagg ctattttaat cgctgcaaat gtaacaataa ggtaacaaat
    16441 ttgttaccca aaacaaatca agtagctaag ttcacactta aacccaaatg aaaatattgg
    16501 gttaatgttg taactttact aaaaaaattg acgccacaat gaagaaacta ataattgcaa
    16561 ttgcaataac acttgcttcg ccctatttgt atgcacagga aacatatcac catatctcaa
    16621 atatcggtat ctacgagttt attgatgagc taaatacgct tggcattgta aatgtaaata
    16681 ccagcgttaa gccttacagt cgtgatttta tcaaaacgtt actaacacag gcaaacgaac
    16741 agaaagaaaa gctgaataac cgtcaaagga aggagcttga attttacttg gccgattttg
    16801 ataaggataa tgctgctaag gggaaaaaac gatttgatat cctgtatcga tccgactcac
    16861 tttacagtat tacaataaat cccttgctgg gtgtaaacac cattaaaaat gatagtgcat
    16921 ttgcctataa ccgttgggtt ggagcagagg tgcatgggca tgccggaaga tttggttttt
    16981 atgcaagtct ccgcgattat gcccagaatt ataggcttgc gatgccaacc tatttgaccc
    17041 gagagcaagg cggtaactac aaggtagatg gagatggggg tgaatttagc gaaatgagag
    17101 gcggtataac ctatggatgg aaatggggca gcataggaat gattaaggat catgttcagt
    17161 ggggctccgg ttataacggc acaaatattc tgggggggcg cacaccatcg tttgccatgc
    17221 taaaacttaa tgtaaaacct gctaggtgga tagagctgaa ctacattcac ggttggcttg
    17281 tatcgcaggt aattgattcc gcaagaacat acataagctt tggagaaacc cgaaaggttt
    17341 accacaacaa gtacatcgcc gctaacctta taacctttaa accctacagg gggttaaacc
    17401 tttccgtggg gaactcaatc gtttactctg accttgaggt tcaacccgcc taccttatac
    17461 catttttctt ttacaaatca attgaccatc acctgaatgg catgagcaac caggttgggc
    17521 aaaactcgca gatgtttttt gatatctcat cgtacaacgt taagcacctt cacctttacg
    17581 ccactctttt cctggatgag ctttcgatga aaaatatgtt taaccccgat aagcacagca
    17641 atttttacag cctgaaagcg ggcttcaggg tgtgggacct ccctttgagc aatttatcat
    17701 taacctttga gtacacccgt actaatccgc tagcatttaa gcatattatt gaaacaacca
    17761 catacgagag taacaggttt aacctaggcc attaccttac cgataatgcc gatgaaatac
    17821 attttgccct aagttatcac cctacccggg gattacagct attcggaagt tttaccaaaa
    17881 gccgaaaagg tcccgattat acaaagatta caaccgaacg actaggactg cccttcatgg
    17941 agcaagtaga gtgggagcaa acgttctggc aagtgggtgc tcgataccag ataattaacg
    18001 atgggtatat ctttatcgcc ggaggggtta acaacatcaa gaactacact agctataagt
    18061 acaccccaaa ctttatgctg ggcaagcaga tgcaagttga agctggcatt tgctggggct
    18121 tttagttgct cctataccgc ttgaatacaa tattataaac ggcaacaaaa aagtacttca
    18181 ggtcagtaaa cagtggatgc ttatcgtaag catcaaggta tttgatttcc gaagccatta
    18241 tctcctccag ggttttggga ttatcaacgt aaaatggtgg aattaggcct ggtttgtact
    18301 tgattctcct ttcctggagt tccttggagt ataggttaaa gtaatgctgg cttaaaggcc
    18361 ttacccccac tatttttagt tccccccgta ggaggttaat aatcataggt agctcatcaa
    18421 tccatagttt ccgcatgaag cgtccaaggg tcgaaaccct aaaatcgtcc ttaaacttgc
    18481 caccctcctg taggttatgt ttgcggtaaa tgtattcctg taggtactcc gagtatgggt
    18541 gcatggttcg gagcttgtaa accttgataa atttgccacc cttcccaata cggcgaagct
    18601 ttatgagagg gccataggta ggctccatgt caaagtatgg cttgcggatt ttttgggaaa
    18661 ccacatagtg cattttgtca atttcctgct catcaataac atcgaaaccg catgagtaaa
    18721 gccttcctaa aagctcggcc ctgcttatca ccctattctc accacgtgta agcaaaaagt
    18781 atattccctt ggtaaatgaa aacttaggga aaactcgttt taggatgaaa tcaaagagat
    18841 agtatatgta gttaagtatt ggtgggaatt tactaagtat tcgtttctta cgctgttcct
    18901 gtgtttcggc catgcaaaca aacacaccac caacaggaat tttctcgtta gcggcctcaa
    18961 agaacttgtt aattcgccta atatcgttaa cccgcttaag gtttataatg gttttaaagg
    19021 tattgctggg ctgatttaaa aggttgaagc tggttgttgt ggcaataata agcgttttat
    19081 cgctaaacag gttggtatgt tgctccagaa aacagtacac atcgcggcta aaatcggaga
    19141 gtattgcctt ttgaactgtt ttaacacggg taggatcaat gggctcctcg ctgatagtgg
    19201 gtgctggccg aggcctacgg agcgagcgct ccaccttgcc caatacatcg tcgggcaata
    19261 cctgggtttt ctttagaact ccccataagt tgtaaataaa cagttcagcc agagtggtga
    19321 atgctattgt actaaacaca atgaaacgtg agtagtaatc gttgcgggtc aaaaagatta
    19381 aaatcgaaat aattgcaagc actatgaggt tagaagtaat aatattcttg gataagtgcc
    19441 ttattgagta agaggtaaag gagtatttat tgtagatgag cgaggttaat atccaggtta
    19501 acgtaaaaat taaaacgcca ttcccatagt tacccaaata cgtttggaac gaaaccccct
    19561 ttattacagt ggttagccaa aaggaaacgc caattaggaa gatatccagc aaaatcagga
    19621 ttgatactcg cctattcatt tactaaaatt gctaattatt gattaaaggt aacacatttt
    19681 tagcaaacta ctaattattc taattaaaaa atggggttgt taatttactc taaacccttg
    19741 caacagatgt aaaaatgatg ttgctttgcc aaaaaattaa agtgcatgct atccattagg
    19801 gaaatataca aggagggata tgggccatcg agcagtcata ccatagggcc tgcccgtgct
    19861 gcagagataa tgaaacagcg ccatccggag gccaactact ttagggtatc gctttatggt
    19921 agtttggctc taaccggcaa agggcaccat accgatagtg cactacgtaa aagtttttta
    19981 ccaaagcagg tagatattat ttggcgaccc gaggaaagcc ttccgattca tcctaacgga
    20041 atgattattg aggctttttc ggaagaggac ggtcagctta tcgacttttg ggaggtttac
    20101 agcataggcg gtggcgattt aaaagataag acaggagttc taaataataa ccctgtttac
    20161 cggctgacaa ccatgaccga gattttagcc tggtgcaaaa gcgaggggaa aagcctatgg
    20221 gaatttgttg aggataacga gggtaaagag atctgggatt ttttagcaaa ggtatggcat
    20281 accatgcgcg aaaccgttaa gcgtgggatt gataatgagg gtgttctacc agggcctata
    20341 aagctggctc gcagggcttc agcccagtac accaaagcat taaacgccca tggaacaatg
    20401 aaacatatag ggttgctctg tgcttttgcc cttgcagttg ctgaggagaa cgcggcaggg
    20461 gggaaaatca ttaccgcacc aacctgtggt tcgtcggggg tgctacccgg tgtgcttttt
    20521 tacctcaaca gcgaggagca tattacggag cagaaaatgc ttagagcgtt ggccactgca
    20581 gggttaatag ggaatctggt aaagcataat gcatcgattt cgggggctga ggtgggttgc
    20641 cagggggaaa taggcacagc ctgcgctatg gctgctgcag ccatgaccca aatcatgggc
    20701 gggtcgccca cccaaattga gtatgcagcc gagatggcca tggagcataa ccttggacta
    20761 acctgcgacc cggtaatggg atatgttcaa atcccttgta ttgagcgcaa tgccattgct
    20821 gccactaaag catactcatg cgcagtttac gccctatcgt ccgatggggg gcataaagta
    20881 tcgttcgata aggcggttga aaccatggcc gaaacaggcc gcgatattca ggtggcctac
    20941 cgcgaaaccg gtattggtgg acttgctaaa aattgggagg cttttggcta gtgtgccgat
    21001 ttatcgagct cttcccaaat aagggcagca gctccgagca cagcagcatt tttatcctgc
    21061 aaacctgaaa gcaaaagttt taccttgccc ttaaaaaccg ggaacatgta atcctccatg
    21121 taacggcgaa caggatccat tagcagctca ccggcattgg ctaaacctcc aaagaagaaa
    21181 atggcctcag ggttaagtat agccactaaa tcggcaaggc gagttcctaa aataagacca
    21241 gtatactcaa atgctgccct agcaagcgga tcgcctgttt tggccgcctc agtgatcatc
    21301 ttggcagtga gcttatcgta ggtaacgttg cggagcatgc taggctgatt gctatcggca
    21361 atcatcttga aggcggttcg gcggaggcct gttgctgagg cgtaggtctc aaggcatccc
    21421 ttctttccgc atccacactg ccggccattt gccttggcaa ccacatgtcc aaactcgccg
    21481 gcaaaaccat cgtgacctac aaccagcttg ccatcaacaa caattccgct acctaaccct
    21541 gtacccaaag tgataacaac aaagtttttc attccctttg ctgccccaaa tagcaactca
    21601 cccagtgcag cagcattggc atcgttggta accacactag gaactccagt taataaagta
    21661 atctccttac ataaagggag tatccctttc cactccaaat ttggagcatc aactatagcg
    21721 cccagattat atgaaccatt aggagaacca acaccaacgc caagcaacct gtgttcatga
    21781 ccaagctcca tgagcagctg ctcaatagcc ccggttaagt tttttacaaa aacatcaaag
    21841 gtagcattac agcgtgtggg aagggtactc tcggcaagta tatcgccatt acggttaact
    21901 attcctatta ctgtgtttgt accaccaata tcgatacctg ctacaacctc agccatacat
    21961 ttgatcaaat taaactatag ctaatctaac tcttcgtaat ccacgtattc accctgattc
    22021 ttatcgataa tctttttttt ccgggtgaat gaaaaatttc gccccttgct gttatcggac
    22081 gattgttgac ctgtttcctg gttaattttt ttatacaacc gccttaacca aagccttagg
    22141 atgatgcgtc ctatcaaacc cagtaaaaaa agggtcaaaa agaagtaaaa aatgaatttt
    22201 aaaaatgcta tcattattct gttgaaacgg tgcgcaaaga tagctattta tttaatggca
    22261 aagcatataa ccccattctg ttttgataaa gtaacaaatt gcttgttcct agaatttacc
    22321 tcaacaattg gaatattgcc cgatataggg aaaaagctaa gaggtttgcc actaaaatcg
    22381 taaccaaaaa tactttcatc cctgtttgtc aaaaccaggt tattgtttgg catgacctgt
    22441 aggtttccgg tatcgaaatc gcctattgac gatagtatcg cattttttaa tttgccttcg
    22501 tttgaatata tgcttaccct attcctatcg aggataaaat aatttttccc tgccatgata
    22561 aaggtatggt tgggtgagta tggttcaagc acttgacttt cgaccctgcc atcaaaaaat
    22621 agtttcttga ttatgcccag cgtatcggtg gttagcaaat atgacccttt ccccgaattt
    22681 cctaccaggt aaaattcact gttttttgct ttggcaaaaa atgattgagg ctttaaccgt
    22741 tcctcgccac gacggttcag taaatacggt cggttttggt caaaaatcaa tatataatcc
    22801 ttaccctgat tgctaacgta tcctattcgg ctactaacaa ccgtttctgt tttaaagcta
    22861 agccaaccgc ttacgggttt tccgttagtg tcgtagcagt aaatcttttt atcggagcaa
    22921 gccacaataa accggtagtt acggttactg tcgtaatcaa atgccgataa tggggaggta
    22981 gctctagcag gcaaggtcac cggaaaacct tcaacatcat ttccattgcg gtcaaccagg
    23041 tatatcctgt tgcctgctgc aaaggccatc tgtaactttt tgtttcgata aatatctact
    23101 tggctcacac tgccaagaat tggagcccca atctttcgtt tccagagtat atgcccatca
    23161 ttactaataa gatagattga gttttcagag tcctgaacca aaacctccgg ctgtccgtta
    23221 agatggttaa ccaacaaatg tggtttgcca acaggttcag tgctgagctt gcaaacccag
    23281 ttcacatttt taagcgagtt gggggtttcg gtcaaatcgg cctggctgct ttttatggca
    23341 agcatagcaa aaagcttttg gctaccgccc actacctgcc aaataaagtt atggctggag
    23401 agagtttttc ctttgtatag ctgggttggc ttaccatgca agtgtaattc gcttaagggg
    23461 tatgaatcca ttcccttgct ataaagaatc agattcgatg atgtggaaat acgttgctga
    23521 atgctcctat agccttctgt tctggtaagt gtgttattac gcaaatactc attaattatg
    23581 aattcaagcg aactaatgct tgagccgaaa accaaaacgt tatcgattac cgtcacaaac
    23641 tcatcgttta ctttcaaaaa gctattaccc aatagggtag ggataaacca tttaataggg
    23701 ttttcaacaa tagggaaact tttttgttga tcgggcttaa actcatgttt gacaatggtt
    23761 ttggtacctg caaacgattc aagctgctgt agggtagttg aaccgctctt ggtgtttgca
    23821 agaataaacc aatctgtgct atcgttttgt aatttggccg atagtacagc aacttcgtta
    23881 tcgaggtttt tcactaaaaa ctcatcgatt gaaacccctg tatgggctaa gaaacctgaa
    23941 aggtttctat tgtatggttc agttaggcca aggttatcaa tataatcgct atagtcggcc
    24001 ataaagttag ggatattggg caggccatac caggtaaaaa atgatgttga agcaggcatg
    24061 tagccatgca aagaaaaagg aacagggctt tgacgggtaa atattttata gtaatcgcca
    24121 ttggtggctg aactcacaat aagcccattg gcaattacca tggtaggcga tacttgaaca
    24181 tcgagcccaa tccagcttgc aaccccctta agagcaagcg aattattttc aagcgataaa
    24241 gttttggaga gctcattgat gtttacaaag acatttgctg caacgccagt tgcggctgtt
    24301 tggtaaagcg actgaaactg ctcatcggat tcaacggcag catcttcctt cagctttttg
    24361 accgattcaa caagcaattc ctggctattg ctaaccagta gaagattgcc gaacacggta
    24421 aaagttaagc tgaaatagtt cccttcagga acctcaacat aaattgcgct gttaccggca
    24481 atttgtatct cctcggcctt agcataccct aaactttgaa gtttgataag ctctagaata
    24541 tcgctttggt taatctcctt gggaacttta tgggccagta aaaactcgct gccctgcgga
    24601 tggttgttgt aaaaagctac tgcagtatta gtgcctttaa tggctgttct aactgtattg
    24661 tccgattttg ctgcgctatc caaataatta agcatgtctg caaaactttt aacgacatcg
    24721 ccccgttcaa gggttttcca ccaaccattt gactttacag agttagtaaa caattcaaaa
    24781 tcatcaatat tcaaaatcaa cgaggcattt agagggatag catcaattgg gttgaattgc
    24841 cggtaatcct gtttgtttac ccaatggtaa aacagaaata ctaatccagc aatggcaagt
    24901 gttagaaata ctatggtagc tatgatgcgc ttcatcggtg taactgtttg gcgtgctaaa
    24961 gttacaacta atttaggaag gataagctga tatttggtgt ttagtgagtg gggaagtggt
    25021 tgatagttgg ttgttgatag tgaatatgaa acgccaaaca ctaaacacca aacacgaata
    25081 atcctcccct gaataactga acttaaactt ttgattgtct gaaaaaaact ctaattttgt
    25141 gagatttttt tggaagcgat tatctacaca gattttagta tttaacagcg ttttatatgg
    25201 gtaacctaaa atttaaagag tacaagggac ttgatttgcc tcaagtgaac aaggatgttc
    25261 ttaagacctg ggagcagcag aacaccttta agaaaagtat tgaaactcgt aaagggcacc
    25321 ccaagtttat tttttacgaa gggccaccat cagccaatgg aatacccggc atccatcacg
    25381 taatggcacg caccattaag gatatttttt gtcgatataa aacccaaaaa ggttttttag
    25441 ttgagcgtaa agcaggatgg gacacccatg gtttgcccgt tgaacttggc gttgaaaaaa
    25501 tgcttggtat aaccaaggag gatattggca ccaaaatatc agttgaggag tacaacaata
    25561 cctgccgtaa ggaggtaatg aaatataccg acctatggga ggaccttacc caaaaaatgg
    25621 gctactgggt cgatatggac gacccctata tcacgtacga caaccgttac attgagtcgc
    25681 tttggtatct tttacaaaag ctttttgaga aaggtttact ctacaagggg aaaaccatcc
    25741 aaccatactc tccggctgcc ggaaccggtt taagcaccca cgagcttaac cagcccggat
    25801 gctaccgcga tgttaaggat accactgtgg tagcacagtt caatgttatc aggaacgaga
    25861 gtagccagtt cctttttgac ctaaccgata ccccagatgt atacatactg gcttggacaa
    25921 ccacaccctg gactctacca tcgaacaccg cactggcggt aaatgccagc atcaagtata
    25981 gcatcgttag aacctttaac ccatacagcg gtgagccatt gactgttgta ctggccaagg
    26041 acaggcagga ttattacttt ccggcagcaa atgccgagct gtcgttcaac gattatcaac
    26101 caggcgacaa aaaggttccc tttaaggttt tatcggaggt tgagggttca aagcttgtag
    26161 gtatccgata ccatcaacta ttcccctggg tgaaacccga gggcgacgcc ttcagggtga
    26221 ttgccggcga ttttgttact accgaggatg gtaccggtgt ggttcatatt gcaccaacct
    26281 ttggtgccga cgacgaccga gtggcaaagg catcgggtat tgctccgcta atacttcgcg
    26341 acaagtaccg caatcctgaa cccatggtcg ataggcgcgg aagattttac ccaatagagg
    26401 acctcgaccc tgactttgtg aaaagcaacg taaatgttga cctttacagg gagtttgccg
    26461 gtagatttgt taaaaacgag tacgattcaa gcctgcctga ggatgcacca acacttgatg
    26521 ttgaccttgc ggttgcccta aaaaaggaaa ataaggcatt caggattgaa aaacacgttc
    26581 acaactaccc gcactgctgg cgcaccgata agcctgtact ctactaccca ctcgatagct
    26641 ggtttatccg tgttacagcc cttaaggata ggatgattga actgaacaaa accattaact
    26701 ggaaaccggc cagtacaggt accgggcgat ttggcaaatg gctcgaaaac cttttggatt
    26761 ggaatctttc ccgttcgcgt ttctggggta caccactgcc catctgggtt tcagaagatc
    26821 gcagcgaaat gatttgtatt ggttccgttg cccagcttaa ggatgaaata gaaaaggcta
    26881 ttaaggctgg gttcatggat aagaacccgt tggccaacta caaggagggt gactttagca
    26941 aggaaaacta taacagcttc gacctgcatc gcccatttgt tgataatatt gtgcttgttt
    27001 cgccaagcgg gaagcgtatg ttccgtgagc ccgacctaat tgacgtttgg tttgactctg
    27061 gagcaatgcc atacgctcaa attcactacc cctttgagaa caaggataac cttaacgagt
    27121 tgttcccagc cgattttatt gctgagggtg ttgatcaaac ccgtggctgg ttctatacac
    27181 tccatgcgct gtcaactcta cttttcgatt ctgtagccta caagaatatc gtttccaacg
    27241 gattggtgct tgacaagaac ggtaacaaga tgagtaagcg ccttggcaat gccgttgacc
    27301 cgtgggaggt aattgaaaag tacggttctg atcccttacg ctggtacatg attaccaacg
    27361 cacaaccctg ggataacctt aagttcgata ttgatggtgt tgacgaggtt aagcgtaagt
    27421 tctttggaac tctttacaac acctactcgt tctttgctct ttacgccaat gtggatggat
    27481 tttcatatgc tgaggacgaa attccggtta gtcagcggcc cgaaattgac cgctggatac
    27541 tttcgctgct taacagcttg gtgaaagagg ttgaggaggc ttacgaggac tatgaaccta
    27601 cacgtgctgg ccgagcaatt caggattttg taattgagca gctcagcaac tggtatgtaa
    27661 ggcttaatcg taaacgcttc tggggtggcg agtacgatac cgacaagatt gcagctttcc
    27721 aaacccttta tacctgtttg gaaacagtag ccatgctggc tgcccccatt gccccattct
    27781 acatggatca gctgttcact gaccttaacc gtgttacaca tcgccattca gccgaaagcg
    27841 ttcaccttgc gaacttccca aaatgcaacg atgaattgat tgacaagaac cttgaggaga
    27901 agatggaaat ggcccaatca atttgctcac tggtacttgg attgcgacgt aaggttaaca
    27961 tcaaggttcg tcaaccacta caaaagatta tgattccaat ccttgatgaa aggttcaggg
    28021 agcaacttgc agcagttgaa aacctcattc ttagcgaggt taacgttaag gaacttgagt
    28081 accttagcga ctcatcgggg attctggtta aaaagataaa accgaacttt aaaacccttg
    28141 gccctcgcta cggaaaggct atgaaagcaa tttccgaagc cattgccaaa atgacacagg
    28201 aagacattgc tgcttttgag cgtaacggaa agttcaccat aaaggcagcc gagggagata
    28261 tcaatctaac ccttgaggat gttgaaattc tctcggaaga tattccagga tggcttgttg
    28321 caaacgaagg acgctatacg gttgcccttg atattaccat tactgaggag cttcgcatgg
    28381 agggtattgc ccgtgagctt atcaataggg ttcaaaacat gcgtaaggat tcaggctttg
    28441 aggtaaccga taaaatccgc atcaaaatac agaaacacga caagctaaac agggctattg
    28501 aggttcacaa gagctatatt tcaacgcaaa cccttgcccg tgaaattatt ttagttgatg
    28561 aggaactaaa tggaaattct aacgtaatag agttagacga tgaactttca actgctattt
    28621 ctgttgaaaa gattgaatag atgagttggt tttttactac aattttttta tatctttagc
    28681 aaaatttaaa aagccatggg agagaagacc agatattcgg atgaggagtt agcagaattc
    28741 aaggaaatta ttctgcagaa actcgaaaag gcacgtaggg attacgaaat ccttaagagt
    28801 gccattacca tggatgagag taacgatacc gaggatacct cgccaacctt taaggttttg
    28861 gaggagggtg cttcaaccct atcgaaggaa gaggctggcc gcttggctca acgccagctc
    28921 aagtttatcc aacatcttga ggctgccctg gtacgtatcg agaacaaaac ctatggtatt
    28981 tgccgcgaaa ccggcaaact gatctcaaaa gaacgtctac gtgctgttcc acacgcaacg
    29041 ctcagcattg aagccaaaaa tagtcaacga tagctgtaag tgttaatatg tattactcag
    29101 gaaagcgacc atttggtcgc ttttactttt tatattagtt ttacgccata aacagttaac
    29161 ctatgaattt caataagaaa catttagcct ttctaataat ccttcttatc cttgtaattg
    29221 atcaggccct caaggtttgg ataaaaacca actttgccat aggcgatgag atgagggtaa
    29281 ttgggaactg gttcattctt cactttactg aaaatcccgg tatggccttc gggttaaagt
    29341 tttggggtat ttggggtaaa attggcttaa gcatattccg tattgcagca attgttgcca
    29401 ttgcatggta tttaaatcat cttattaagc aacaggtaaa aacgggaatt atagtaggcc
    29461 tttcgctcat ccttgccggt gccattggaa atattatcga tagcgcattc tacggttatc
    29521 tgttcgatag tggtaccacc tatagctcgg agtttaacca gtgggtatcg tactatggca
    29581 tatcaaagct aaactttgga ggatatgccg gattccttca gggatgtgta gttgatatgt
    29641 tctacttccc aattatcgat actgtgctac ccacatgggt tcccattatt gggggtgagc
    29701 acttcgtttt cttcaggcct gtttttaaca ttgccgattc atccataacc atcggggtga
    29761 tatacctgct gctgtttcat aggaatactc tgtttgggca tcaacctaac caaaaaccat
    29821 aatccatcat gaaccgcaaa gttgtaatca ttatgggctc aaaggctgac cttgattggg
    29881 ccaaccaaat tgttaacgtg ctaaagggtt ttggaattga atccgtaaca cgcattgcat
    29941 cggcccataa ggtgccgcta aaatgctatg agctgattaa acattacgaa aaggaaaacg
    30001 tagtgtttat aaccattgca ggcatgagca acgccttaag cggtttcacc gatgctcaaa
    30061 cccactgccc agtaattgcc tgcccaccat acagcgataa gtttggtggt gccgatgtgt
    30121 attcgagcct tcgtatgcca tcgggtgttg caccacttac aattcttagc actgaaaacg
    30181 cagcccttgc tgccgcaaaa ataattgggc tttccaatcc cgatattcag aaaaagattg
    30241 ctgaatacca ggaaaagaag cgaattgaga ttgaagaggc cgataagaat attcagtaat
    30301 tgaagctgat gggactagtt ggggttgata tcgaccacgc tgcatcaatt attgccaacg
    30361 ggggagttgt agcttttcct accgaaacgg tttatgggtt gggggctaat gcccttaacc
    30421 ccaatgccgt agcaaaagtt tttgcactta aggagcgccc ctcgttcgat cccttaattg
    30481 ttcatattgc ttcagctgaa agcataaacc ttttatcatc aagcaacgac cccagagtat
    30541 ttcagctagc agaacggttc tggccaggcc cactaaccat agttgttccc aaaagtaccg
    30601 ttgtccccgg cattgtgacc tccgacctcg atacagttgg catacgcatg ccaaaccatc
    30661 ccattgccct ggagcttatt aaaaaatcgg gatgcgtaat tgcagccccc agtgccaata
    30721 agtttggaag ggttagcccc acacgcgcgg agcatgtgct aaagcagtta cctgaggtgg
    30781 actatattct tgatggaggg agaacaaccg ttggaataga atcgacagta ataacgctcg
    30841 acgaggaagg ttttattatt ctgcgtcatg gtgcaataac ccataatcat ttggcacagg
    30901 tgctgaaacc ttcaggtgcc cataggcctt taaaaggctt agcatcgccc ggactgctta
    30961 aatcgcacta cagcccaaac aaaccactat acattgctgg tgaaaccagc aaacccattg
    31021 acccatcaag agcgggatat attgcctttg ggaaaaaacc agaaggtaac tacaagcgtg
    31081 tagagtacct atcggaaacg ggcgatatgg ttgaagcggc agcgcacctt tttgaaaaaa
    31141 tccacgcctt tgaggattcc gatgtggaat acatagttgc cgatcctgtt cctgaggtag
    31201 gcatcggaat tgccattatg gacaggctcc gtaaagcggc ttacaggtat aagtaaagtt
    31261 gttcgtttat agttgatagt tgttggttga tggtgaacct aaacctttac tctttatcct
    31321 atatccttaa accttgaacc tttatccttt atccttaaaa cttaagtatt agttgcatag
    31381 gcaagagtgc caacgctaac tttaacatta acagaatcga taagggtttg ggtgcaggtc
    31441 tcaagtgtgg cccctgtggt aataacatcg tcaaccagca ggacatgccc tccttctatt
    31501 ttttcggggt tgcagatagt aaatgccccg gcaatattct gcatgcgctc catgcgcgtt
    31561 ttcttggttt gggtttgggt ataaatgttt ctgagcaaaa catcggtcac caccggaacg
    31621 cccatggctt ccgataatcc ccttgcaatg gcctcggcct gattataacc gcgttcctta
    31681 aatcgtttag ggtgtagtgg cacagggatt attgcagtaa ttggcttata tagctccgag
    31741 cttttcagct caaggccaaa ctgcctacca agaaacacgc caatatcctc tcgcccttgg
    31801 tattttaaca tatgcaatag cttacggtag cggctaccct tgcggaagta aaagaatgag
    31861 caagcatgct ccacattcac cctaccccaa aagatttgcg atactgggtt gtcgtcccag
    31921 ttccagaatc gggtacgggg aatatgatac aaacaggaag tgcaaagcac ctcctcgtat
    31981 agcgaaagac tatcgtggca aatggtgcaa acgttagggt aaaaaagatt tataaaatcg
    32041 tatgctacac gttttacccc atgtgtttag ttgcttcggg tccactccac ctctttccca
    32101 atgaaagaga aacctatata acccttaacg tggagcaagt tatagttacc atcgtcaaac
    32161 cacatgtagc acttgtaggt ttttccctct ttggggttat agatagtgcc atcaacccac
    32221 tggttgtcct tgtaaacaaa gccatcgagc attctcaaac ccataattgg cctattctgt
    32281 agcttaggtt caggattctt atcgtcaacc ttgggtttcc catccttatt gggttcctta
    32341 agccaagtaa tctcgccata aaattttccc ttggcatcct tcttaatggt taccttggag
    32401 tcgccatact gtgtaagcca tgtaccaaga accttgtcct ggtgttgtgc cattgccggc
    32461 actattagca taatagccaa tgatagcaaa aaaagctttt tcatagtaat ttaggttggt
    32521 tatgaatttc aatattagta attttttatc aattatttag cctcaacaat aaaacagccc
    32581 ggatatttgg tttgagcgga ttgttttacc ttttcggcat cggaacggga tgtaaacggt
    32641 cctataagta acttgaaaag gcggtcgttg tttaccgcaa caacctgaac gcaagttttc
    32701 tttccaaact gctcctgcgc atcggctagc agcaaaaaga tattggtttg ctcccggaaa
    32761 ctgcccagct ggacatagaa tccttttttg gtttgctttt gaacttcgac cgagaaatac
    32821 tctatcgctt cggaaggtgc agcttgttgg ttggttttaa tctcagtagt ttgtaaattg
    32881 gtagttttct tatccccatc agttactgcc gttttcgata cctcctcttg ggaattaagc
    32941 ttaatcccct ggttatctac aacctcaatt cttacccgtg ctatacctgt accaatcatc
    33001 cctaaccttt ccgctaccga tttggaaaca tcaattattc tatcgggtac aaaagggccc
    33061 ctatcgttta cgcgtgctat tgccgtttgg ttattatcca ggttggttat tttaaccagc
    33121 gaaccaaacg gtattgacac gtgtgcacag gtagctttca ggtgactgta gcgttccccc
    33181 gatgcggtaa cccttccctc aaaattatcg ccgtaatacg atgcgttgcc ctcctgaaca
    33241 tatcctacct gagcattgat gcatatgggt aatagggcga ttattgctgt aaataggctg
    33301 cgcattttat ggtatggctt gatttagaaa tttaaccagt ggactaagcg tttcaaacac
    33361 ttcaattgcc ctaaggtcga aatcgggatg aaagagttca tcatcggtaa aatcgcgtga
    33421 aggggttatg tgtttcaacc tgagaaatag atcgagttcg ggattgaact caaaacctgc
    33481 aggtgtgcgc ttagcctgct caactccctc ccaagtaaag gttgatttga acttagggtt
    33541 gttaactatg gaaaggaact cgctcctatt ttcaaccatt gccgtgcgta ccgctttcat
    33601 aacaggcgca ggggccatgt acaaacctcc cgaagcaaat gaacccccgg gctggatatg
    33661 gatgtaatac ccacaaccac tactttttcg gccacccgga gctataaagg ccccaaagtt
    33721 ctccttgtaa ggatttttgt tagtggaaaa gcgaacatcg cggtaaatac gaaagatgca
    33781 atcgcgtggt tcaagagcac ctatcgattt atcgaaatgg ctaatacctg caatcagtgt
    33841 gaacacaagc cgttgaaaat cggtttgggc cagcttataa taattgcggt tcacctgaaa
    33901 ccactcacga ttattgttgt ttgagaggtt tacaagaaaa ttaacggttt ctttttgtag
    33961 tccgggcatg ggggagatgg tttactaaca aaattagcaa aatattttat gacccaaata
    34021 aaaaagccac tcctgtggag tggctttctg tcggctcggt ggggatcgaa cccacgaccc
    34081 catgattaag agtcacgtgc tctaccagct gagctacgag ccgttatttt tgtggctgca
    34141 aatttagtat cttttttatc caacacaaaa tgtcatccaa aaatttactt tctttacaaa
    34201 aaatttttgt taaacacttg taaattcagg aaagcggaat ggaacgcaga actattctaa
    34261 ttaccggtgg cgcaggattc attggctcgc atgtggttcg ccgatttgta aacagttacc
    34321 ccaactacct gattatcaat tttgataagc taacctatgc tggtaacctg caaaacctta
    34381 ccgacattga gggcaagtcc aattacaaat ttattaaagg cgatatagcc agcgaggccc
    34441 atgttgacaa ggtttttagc gagtatgcca ttgacggtgt tatccacctg gcagccgagt
    34501 cgcacgtgga caggtcgata accgacccca tggcatttat ccgtacaaac atacttggaa
    34561 ccaccattct tctgaacgca gcccgaaagt tttggaatgg caactgggaa ggaaagcttt
    34621 tctaccacat ttccaccgat gaggtttacg gcagccttgg ggctgaaggg ttcttcactg
    34681 aggaaacccc ttatgacccc cgcagtccat actccgcttc aaaggcatcg tcggatcatt
    34741 tggtaagggc ctacaatcac acctatggcc tacccgctgt aatatcgaat tgctccaaca
    34801 actacgggcc aaaccagttt cctgagaagc ttataccact atctatccac aacataaaga
    34861 acagtaaacc catcccaatt tacggtaaag gggaaaatgt tcgcgactgg ctgtacgtgg
    34921 aagatcatgc agccgctatc gatctgattt tccataaggc aaagcctggc acaacctaca
    34981 atattggggg taacaacgag tggaagaata ttgaccttat cagactgcta tgtcaaataa
    35041 tggacaggaa actcggtaga gcccaggggg aatcggaaaa actgataacc tttgtaaagg
    35101 acagggctgg tcatgactta cgctacgcca ttgactccag caaaattcag cgcgagctgg
    35161 gctggaagcc atcgctaact tttgatgtgg gcctggaaaa aaccgttgaa tggtatctgg
    35221 ccaatgagca atggcttaac aatgtggtaa gcggcgatta cctgaaatac tacgagcagc
    35281 agtacggagg gcgctaggtt agtgtatagt aataggggca ttggagaatg tgaggaagtt
    35341 agaggagtat agagcgttaa acgtaagccg taagccgtta aacgaaatcg agcaacccct
    35401 attgtcatta ggagcctagc gaggaatctc atttgaaagt ggaattgaga agaagttata
    35461 aagaagcttg aggaagttag agaaatgtag agagagccgt ttaaataaac ccgaacaaca
    35521 aataactaca cccagagggg cagggcaaag tgtattttca ctaccaacta tcaactacca
    35581 actatcaact aaatttttta cctttgcttg attaaaagtt tatcatggcc gaaaaacccg
    35641 atagcaggat tgttgagttt atcaacgagc atcatgtttt aacgctggct acaagtttca
    35701 acgaggaacc ctggtgcgct aactgtttct atgtttacat ggacgatgaa aacagtcttg
    35761 tgtttacttc cgatttcgac accaaacaca tccaacaggc cagtcataat atctacgttg
    35821 cggggactat tgtgcttgaa acaagcatca ttggcaaaat ccagggagtg cagtttcagg
    35881 gaataatatc acaaccacaa ggcgaactgc atgagagagc caagaaggct taccttaagc
    35941 gttttccaat tgccatgcta atggaaaccc acctatgggt tgttgacctt acctacttaa
    36001 agatgaccga taaccggtta gggtttggta aaaagctaat ttggtcaaag gacctatcgt
    36061 tccagaaata aattcaccca acagtatgat acttgtaact ggtgctactg gactcgtagg
    36121 tgctcatttg ctttatgagc tgtgttcagg ggggcacaag gttagagcta cccgaagggc
    36181 aaactcaaaa acgcattttg tagagtgggt tttctcacta tacaccgcta attcttcgga
    36241 cttactaaat ctgattgaat gggttgatgc tgaggtgtta gattaccaat cgttggttga
    36301 ggctactaag ggcgtatcaa ccgtttttca tacagccgct acggtctcgt ttagcccatc
    36361 gcaggagcag aacatacttg ccaccaatgt tcgtgggact gctaatgttg tggatgcctg
    36421 tcttgagaat ggggttaaaa cgttgtgcca cgttagctcc attgcagccc taggcgaaag
    36481 gaacgacttg ggagtaattg atgagagctg caagtggcaa aaaagtaagg gacagagtgc
    36541 ctatgcccga agcaagtttt taggtgaaaa cgaggcttgg cgtggttttg aacaggggtt
    36601 aagagtggta atagttaatc cttcagtaat tctcggtccc ggacgatgga attcgggtag
    36661 tggacagctg ttcagcatag tatcgaaagg gatgccattt tataccgatg gcgtaaccgg
    36721 ttatgttgat gttcgcgatg tggcaaaagc catggtcttt cttactctaa acaccgaggt
    36781 tagcggagag cgatttgttc ttaattcaga aaaccttggc ttcagggaac tttttacttt
    36841 aatcgctaat gtttccaaca atagaaaacc ttttataaag atacctttgg cattaataca
    36901 tgttctttac ccgctgattt tcattcttac agccggaaga atatcaaaaa gtaacctcaa
    36961 atcggcattt acaaaatcgt actactcatc cgataaaatt aaagcacttg ggtttacatt
    37021 cacacccatt acccaaacgg ttcaactaat ggctgaggcg aaaaagaaaa gttaagaaag
    37081 agaatgctgg ggtcatgaat tcaccatcaa cgaacagcta aaactttcat cctgagcgct
    37141 ctgtctcgcc ttggcgggga gcgaaggatt tcagtaaagg gtaaaagcga taaaggagca
    37201 aagagatgct tcggcgagct cagcatgaca ggatgtttca gagtagtcta tcccaatatt
    37261 tcagnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    37321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnatcctt ccttcccgct
    37381 ttccgtcaaa ccctcttttt gtctgcaatc aaacgctaaa cgctaaatac caaacttgat
    37441 tttcacccat tatctctgaa aatttgtatt tttacttgct taaatcttta aacgttttgc
    37501 tttatggata aaagagaaat tctttcgaaa aaaatactta gtggtgtaga gtacgaccga
    37561 atggtagcat tttggaactt taaatcgcgt tcggtagtag ccctttatgg cacctttgat
    37621 gtttttcacc cgtcggtttt tgacctaatc acctttgcct ctgagttagg tcaggagttg
    37681 gcagtaggcg ttaagcccga taccgatgtt aaggccgaaa aaggcgaaaa atttccaatt
    37741 tataatcagg atcaacgggc tgcaatggtt tcggcgcatc agctggtttc agcagtgcat
    37801 atttgcaatg aaggggttga agatttcctc cgcaggacaa aacctgctgt ggcagtttgc
    37861 tgtgcgcatg catccgaaac cgataagaaa gcccttgagg tggttaagga gtggggaggc
    37921 cgtgtcgaga ttttcacggg tagcaccacc agtatcgacg atatcatttc aaagtataag
    37981 taggtggcta aaactaagag cgtttacgtt tgccagaatt gcggggccga gtcggtaaaa
    38041 tggctggggc gctgcccctc gtgcaatgag tggaacacct ttgtagagga gcggataacc
    38101 aaggagtcca aaagccgacc aggactggta gaggtttccc gcaatgcaat accaaagccc
    38161 ttaaaggaga ttgaaaccag caatgagaag cgcatcgata cccgcatagg cgagctaaac
    38221 cgaatactag gagggggaat tgttcctggt tcaattgttt tgcttggagg tgaaccgggc
    38281 ataggaaaat caactttagc tttgcagctt gccctgggta tggatcacca aaaggtgctt
    38341 tacgtttcgg gcgaggagag tgcccgtcag gtgaaaatcc gtgccgatag gattaaccct
    38401 gccaatcagg attgcctaat cctgaacgaa acgcttttgg agaatatact cacccagatt
    38461 gagaatgttc agcccgattt aattgttata gactccattc aaaccctgta caccgataca
    38521 attgagtcgt cgccgggaag cgtttcgcag attcgcgaaa cggctgccac gctattgcgt
    38581 tacgcaaaaa catcgggtgt tcccgtcata cttattggtc acataaccaa ggatggtagc
    38641 atagctggcc ccaaggtgct ggagcatatt gttgatgtgg tattacagtt tgagggcgat
    38701 cagaactacc tttaccgaat cttacgttca tcaaagaatc ggtttggctc aacatcggag
    38761 attgggattt ttgagatgca aaacagtggc ttggttgagg taaccaatcc atcggagata
    38821 ttactatcgc accgcgatga gcagcttagc ggcattgcag ttgccgctac aatcgatggt
    38881 gcaagaccat ttctaattga gactcaggcg ctggttagca cagcggttta cggtacacct
    38941 cagcgatcca ccacaggctt tgaccagcga aggcttaaca tgcttttggc tgttcttgag
    39001 cgtcgcgcag gttttaggct tgccaacaag gatgttttcc tgaacattgc cggagggctt
    39061 aaggttagcg atccggctat tgaccttgct gttattgcag ccatactatc gtcaacgctc
    39121 gatgttgcaa tacctggcac aacatgcttt gctgcggagg ttggcctttc tggcgaaatt
    39181 cgccctgtga accgtctcga ccaacgtgtg gctgaggcca ataaactagg gatgacccgc
    39241 atgtttgtat cgcgctacaa taccaaaggg ctcgataacg gaaataaaaa ctgtaagatt
    39301 attccaatcg ggagaattga ggagttaatt cgactcttat ttggacggga atccgtggac
    39361 taaaaataat tgatatgaga attgcggtta tcggttttgg agcagcagcc atcgggctgc
    39421 tcgagaagtt aaagaatagc aaccacgagg tgcacgtttt tgagaaaagc aacgatatct
    39481 actcgtccag catatcgggc atacgtgccg atggaaagct atttgtatca accgagatgg
    39541 gaggtgaaat ttatattgat cccttgctgc agcataggtt tgttgagtac tacatcaatt
    39601 ttacggataa gcgtgaggtt gaaaccggca actcattcgc atcggaggat tactaccgta
    39661 aattctacga aaaaggattt ctacccatat catcgcaatt cttccacatt ggaaccgatc
    39721 agctcaagga ggttctcttc aatatctatg agcaattttg caaatcggct aatatccact
    39781 ttcattttag cactgaggta actgaggtaa aagagcaacc tgatggtgta atagttaacg
    39841 gaaccgaaag gttcgatgtg gcggttatag cggttggtag gagtgggcat aagctgatta
    39901 acagaaccat ctcggaatat ccacagctta taaagaataa tactaaggtt gatttgggta
    39961 ttcgctacga gttacccaac cacattgtgg atgaaattaa ccgtgagatg tatgaattca
    40021 aggtgaaata caaaagccgt accggttaca tggttcgtac gttctgcaac aacccatcgg
    40081 ggtttgtggt tactgagaac tatggcgatt tcaccacggt taacggacat gccaagatgc
    40141 gcgaaaaaag cgggaatacc aactttgcca ttctggcatc gcttgtgcta accgaaccgt
    40201 tcaacgatcc cattggctat ggctcataca ttgctaagct atcgaacctt ttggcgggtg
    40261 aggggaaagt aattctacaa acctacagcc actttaagga atccaaacgc actaaaaacc
    40321 tttacagggt tcatccaacc cttgagcccg aaaaatacat tttaggtgat ataaaccttg
    40381 cattcccacg taggattatt gagagtataa ttgatttcat tgaaaatctt aacacggtta
    40441 ttcccggggt ggccaataac gacaatctag tatatgctcc cgaaataaag ttctactcca
    40501 ataggctaaa taatcagatt tcggcaaggt tgaaatttat tggcgattgc tcaggggcaa
    40561 cccgttccat aatctatgca acagcacatg gttacctgat ggccgaagcc atacagtcca
    40621 acaattttaa tgttgatatc tgatgctaaa ggtatccgat ctcacagtat cgttttctgg
    40681 caacccggtg gtttcaggga taagttttag cttgatgccc ggcgaaatac ttggatttgt
    40741 tggcgaatcg ggatcgggaa aatcggtaac atcgttagcc cttatggggt tgttgccacc
    40801 caatgcaaaa gtggaaggtg gttcagcatc tttcagcact gaaaaagggc aggtcgatct
    40861 tttaaaagtt gatagtaaaa cgcatcggca aatcagggga caaggcattg caatgatttt
    40921 tcaggaaccc caaaccagcc ttaacccttc catgatatgt ggtaagcaaa tatcggaggc
    40981 tgttgagcta cattccaatc ttaggggcgc aaaggctaaa gagcgatgca tacagttgct
    41041 tgcggaaatg caattaaccg atcctgctaa ggtttatagc agctatccgc accagcttag
    41101 cggaggtcaa aagcaaaggg ttatgatagc catggcactt gcgggtaacc cccgtatcct
    41161 tattgccgac gagccaacca ccgccctcga cgttaccgtt caaaagagca ttttacagct
    41221 actgaaggat attcgccaaa ggcatcaaat aggcattatc ttcatcactc atgatttgtc
    41281 agtagttgct gaggttgccg atagaattat ggttatgcag aagggacaaa ttgttgagca
    41341 gggcgctgta accgaaattt ttaacaatcc tacacatcca tacacccgtc ggcttattga
    41401 gttccgtaaa cggataaatc aacctgttac tacaacagca agtgtcacaa atgagcctga
    41461 acccattttc cgtgtaaatt caatcaatgt agaatacggt agcaatagct ggtttaaaaa
    41521 gggctttaag gcaattagcg atgtttcatt tactttattt aaaggcgaaa ctttaggtct
    41581 ggtaggcgag tcgggttcag gtaaaagtac cattggtcgt actttgctta aattaatcga
    41641 aacccaaagc ggaaccattg aatataatag ggaaccattg agcagcctaa aagggagcaa
    41701 tctgcttaat tttcgacgcg aggttcagct tatttttcag gacccatact catcgctaaa
    41761 tccgaggcta accataggcc aggccataat ggagcctttt atctatcatg gtttgggagg
    41821 taaaagggag gcccggaagg cggttgagga attacttcaa atggttagct tgccagctga
    41881 cagtattaat cgctaccctc acgagttctc cgggggacaa aggcaaagga ttgttattgc
    41941 tcgtgcactg gttctcaaac caaaaatttt agtttgcgat gagatccttt cagcgcttga
    42001 tgtatcaact caggcccaga tgcttgattt gcttcaggag ctaaaaaagg gaatggggtt
    42061 aacctacctt tttatctccc acgacttatc ggtagtgcgg agtatttgtt caagggtaat
    42121 agttttaaat agaggcagta tcgaagaaca agggcctgcc gaggagatat ttagcaggcc
    42181 ccaaagtgta tatacgcagt tgcttatcga tgcaattcct ggtaaatcgc tttagtagat
    42241 ttctatttcg tttatttcgt aattagtttc attttcacag ttaatgctga tattgcccgg
    42301 cagtattggt ctttgccaat cgtcgttggg cgatatacct agcgaggggt cagcataaac
    42361 cttttgcata aacaaaccaa agatagggag agccatgtta gccccttgtc ctaaggcgag
    42421 gttctggaag tgaaccgaac ggtcctcggc accagtccaa actcctgcaa caagatttgg
    42481 ggttacgccc ataaaccaac cgtcagagtg atgctgggtt gttccggttt ttccaccaat
    42541 ctttccgggc agctgatact tcaaccgcaa gcgaacccct gtaccttggt ttactacact
    42601 ttcaagcagg tttaccatga ggtatgctgt ttgctcactt atggcttcgt ttttcttggt
    42661 gttaaaggtt gccagtacat tcccgttttt atcctcaatg cgcgtaacaa atattggttc
    42721 cacctgaaca cctttattca cataagttcc atatgcacct accatttcat aaaggctgaa
    42781 ctcaaatgta ccaagtatta ttgagggaac aggttcaatg tagctcttta taccaagttg
    42841 atgaatcatt tccaccacag cgcttggcgc aaactgcttc atgatccatg cagagatgtt
    42901 gttaaccgag ttggacaagc cccattttag tgtgaccatt tggccatcgt acttggtgct
    42961 acccgagttt ttgggcgccc aaatggtatc gcccaccaca aaggtttgtg taacattggg
    43021 caccttggtg cagggcgaat atccttcctg catggctaag gtataaagga atggtttgat
    43081 ggttgaacca acctgtcgtt tcccctgccg aaccatatcg tacttaaagt gcttaaaatc
    43141 aggaccaccg acgtaggctt ttacatgacc gttatgggga tccattgcca taaaacttga
    43201 acgcaggaat cgcttgtagt accttatgga gtcaaggggc gacataagag tatcgcgctc
    43261 acctttccat gaaaacaccc gcatgggtat tttctcatta aagttagcca ttatgctatc
    43321 gcgcgaagca cctgaggcaa caagacccct atacctttgc gtttgccgca ttgcaaggta
    43381 aattatatgg tcggcctgtt cgggagtaat atcgtaaaaa attcttcctc cgcgagcctt
    43441 aatctccttg tcaagtgctg gttgaaggtc gtttttaaga tgctcggcaa gtgcctcttc
    43501 ggcatacctt tgcatgcgag agttaacggt ggtgtatatc ttcagcccat cggtgtaaac
    43561 attgtaagga gtgccatcgg gtttggtgtt cttattgcac cacccgtaaa ggggattagt
    43621 ttcccacagc agggaatctt ccctataaag ctgctgcgac cagtagcgct tgggattggg
    43681 ttttgatgca gtcatgaaca atcgcaacat ctccctgaag taggtagcag ttccctggtt
    43741 atgatcctgt gcttgatatt taagtacaat gggcagtttt gatatggaat cgaactgctc
    43801 ttgatttatg taaccgtatt taagcatttg cccaagcaca acatttctgc gttgttttgc
    43861 attttctggg ttacgcactg ggctgtaccg agtaggtgca ttaaccacac ccacaagtag
    43921 ggcagcctga tcaatggtta gtgagtcggg agttgtatta aaaaatgttt tagctgctgc
    43981 tttaatgcca aacgaattgc taccaaattc caccacgttg aggtacatgg cggcaatctc
    44041 ctccttggtg tagttccgtt ctagttttac agcagtnnnn nnnnnnnnnn nnnnnnnnnn
    44101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    44161 nnnnnnnnnn nnnnnntcat tgtaatcaac aaaacttcgg tattccttgt aaatgttgcc
    44221 aagcactatg ttatcatcgg atattacttc cgaggctaca ttgcttttgg ggttttcaag
    44281 ttccttaaag gttggcaatg gcccaaagaa ttccaggcca atcagaataa atattgtaac
    44341 aacaagaagg aagccgaatg ccaatgctcc ccaaaaccag gttttgaact tttgaattgt
    44401 tgcaggatcg tttagcttca tccttacaaa atattttgag ttaataatga taaagttttt
    44461 aggcgattaa ccttgagttc aatataatcg ggaactttat gggtttcggt ttgtgtcagt
    44521 caaacatata gcaattcggt gtaaagttag taaaatatga atgcaaaact gctgtttaaa
    44581 tttttgagga agttataaga gcctcacaag taaacagtaa atcaataaaa aacaaaaaaa
    44641 ttaaagcact attgattaac ataattttgt tcaaaccgta tcaatttctt tactttgtgc
    44701 caacctaact taataatata aggtatggta cctaacctgg taatagttga gtcgcccgcc
    44761 aaagctaaaa ctatcgaaaa gtttttgggg aaagattttg tggtaaaatc gagttttggc
    44821 cacattcgcg acctttcaaa aaataagctt ggtgttgata ttaagaagaa ctataagcct
    44881 cagtacatag ttagcgacga taagaagaag gtggttgagg agctgaagaa agccgcggaa
    44941 caagcccaaa cagtttggtt agcttccgat gaagaccgtg aaggggaggc cattgcctgg
    45001 catctggccg aagtgctaaa gctcgatccc caaaaaacca aacgtattgt ttttcacgaa
    45061 atcaccaaga atgccattca gcaggccatt caaaaccccc gttccattga cgagaacctt
    45121 gtcaactccc agcaagcgcg tagggttttg gatagacttg ttgggtttga gatatcgcct
    45181 atactttgga aaaaggtgaa acctgcactc tcggcaggcc gcgtacaatc ggttgcgctt
    45241 aggctcattg tggagcgtga gagggaaatc atcaatttta agagtacttc attctataaa
    45301 gttattgccg atttcgatgc ccaaactccc tttaaggcag agctatcgga taagttaaac
    45361 accattgatg aggcaagaaa attcctggag cattgtaagg atgcaacttt taaagtactc
    45421 gatgtagtta aaaaacctgc caagaaatcg cctcctcctc catttaccac atccacgctt
    45481 cagcaggaag caagccgcaa atacggcttt tcggtttccc aaaccatgac ccttgcccag
    45541 aaactctatg aggccggtta cattacctac atgcgtaccg actcagtaaa cctttccgac
    45601 ctggccttag ccacctcccg cgaggttgta cttaaagagt tcggcgaaaa atactctcat
    45661 acccgccagt acaaaaccag aaccaaggga gcacaggaag ctcatgaggc aatccgaccc
    45721 acatatatcg ataaggaaaa ggtacctgga actactgcag agcaaaagct gtacgatttg
    45781 atcaggaaac gaacacttgc ctcgcaaatg agcgatgctc aacttgagcg caccaccata
    45841 accattgaag tttccgggtc aaagtaccaa tttgttgcct ctggcgaggt tatcattttc
    45901 gatggtttcc ttaaagctta cagcgaatca tcggacgatg atagcgagga ggcaagcagg
    45961 gatcttttac cccttgtaaa ggttggccaa gccctacaac ctaagcagat taacgccctg
    46021 gaacgattca cacaacgtcc tgctaggtat actgaggcta gtttggtaaa gaaacttgaa
    46081 gagcttggaa ttgggcgtcc ttcaacctat gctcctacca tttcaactat aattgccaga
    46141 ggttacgtga ttaaggagga taggccaggg attgaacgtc cataccaaag cattaccctt
    46201 aaacagggta aaattaccca aacccaaaag gttgaggtta cgggagctga aaaggcaaag
    46261 ctttttccca gcgatattgg aattgtggtt aacgattttc tggtggagta ctttcccaat
    46321 attgttaact ataacttcac tgcccaggtt gaggagaagt tcgatgagat tgcgatgggg
    46381 aagatggaat ggaccaaaat gatagatgag ttctataagc cgttccacga taccgttaaa
    46441 aaaaccgaaa aggagtcgga ttacaccaaa ggggagaggg ttttaggaac cgacccggca
    46501 actggaaaaa ctgttagcgt tcggattggc cggtatggcc ctgttgttca gcttggcgac
    46561 aacgacccca acagtaagga aaaacctcag tatgcaagct tgctaaaggg gcaattggtt
    46621 tcaacaataa cccttgagga ggctttgtcc ttatttaaac tcccgcgtac acttggtagc
    46681 tttgagggta aagaggtggt agttgcagtt ggccggtttg gcccttacgt tcggcacgag
    46741 ggaaaatttt actccctcaa aaaggatgat aaccctttag ctatcgattt aagtagagcc
    46801 attgaaatta ttgaagaggg taggttgaaa gaaaaacaaa gggtaatcaa gactttttct
    46861 gaggaccccg aacttatggt tcttaatggt cgttgggggc catatatttc aaaagctaaa
    46921 agcaactata aaatacctaa aggaactaac gcagcggaat taacctataa cgactgtctt
    46981 aaaataattg aagaatctga aaagaaggaa actacgggca agaaaactac aaaaaccact
    47041 aaaaagaaat agagtaccat atgctagcaa atgagcttac agcataccta gttccttccg
    47101 atgtaacggg attttaccct tttgctgaaa aaaagtattg ttttggcaac caggtgataa
    47161 acctacatga ccaccccgat tccccaatcc gccctggcga tatatttatt gtagggatag
    47221 agtcgttgga ttcagagtca gtatccaacg atattcgtca aatgctttgg gggcttagct
    47281 ccattcatta ctcaaagggg aaagtgtttg atgctggtaa tatttctgca actcttacgt
    47341 tagcccagca gtatcaagtt gtggagtcaa tttcggcaat tattgccgaa tcaaaggcgc
    47401 atctcattct tttaggtggc tcacaggaat tcctatcaaa ggtttatgct ggttggaagc
    47461 aaatccagag cttcatccga atggccatta ttgatagtaa aattgattgg gatggaaaca
    47521 acaacgattt tcatcaggat aactttgtta acactttaat tgatgagccc tacgagaaat
    47581 tgctcgattt aagttttatt ggcattcagg gatactttac tccacagcat accctacaaa
    47641 aggttttgcg ccgcaagcat gattatatca ggttaggtaa cctgcgcgga aatatccgtg
    47701 aggcagaacc cacactgtcc gatgcaaatc tggttagcat tgatatggca gccattcgcg
    47761 cttccgatgc ccctggaaaa gcatttgata atcccaatgg actctatgct gaggagattt
    47821 gtatgctttc ccgttatgcc ggcattggac agagcagtaa atttcttgga atttgcggat
    47881 taaaaactaa tgcacccgaa caaaccatta tgcttgccgc acaggtactt tggcactata
    47941 ttgagggtgt ttcccatcgc agggttgaaa atcccattaa aaacttaccc cataataaaa
    48001 agtatgtagt aagtaccgag gttcaaaatg ttgaaatgat cttttttaga agtgaacttt
    48061 ccgggaattg gtggtttgag ttacctgtaa atagtaaaaa gaaacttttg cgaggaaaat
    48121 tattagtacg gtgcaatgca tcggattatt cacaggcaag taatggcgag atccctcaac
    48181 gttggttgcg ttggtatcaa aaagctcaaa aaatctaatt taaccaggaa ctcattggtt
    48241 aacatttttc aacactaaat ccaatatttt ggaacgttca aaaaaaaatc tgcttaaaag
    48301 gtaaccccta caggcaaaag caggtataaa gcattactgt tacttttgtt aacatatttt
    48361 gttaataaat ttgtatgaat ttaaaatttt tacttacttt acttttcggt tcatataggc
    48421 aaactaattt ttaaaagcta ccaatgagag tatttggaaa ccttgtattt gccactacac
    48481 taagcgtttt gctgtcgtcc catgctttcg gacagcaaga ccctcagttc aatcaaatac
    48541 tctttaaccc gttaactata aatccaggat acgctggaag tcagaacaac atgatagggg
    48601 ttggggccat cagccgcatc cagtgggcag gttttggcga tggtgcaccc ttaagcaata
    48661 cgttaggcat aaatgctcca atatcgccct ttggattcaa gagcggagtt gggttgagta
    48721 tcctgaacga tagctatgga tttaataatg accttggcat taacattgcc tatgctgccc
    48781 gttttaaatt taaattgatt aatggagatt taggatttgg ggttggtgct gggtttgtaa
    48841 ataataagct cgacccaaaa tggaattacc ccgttggtgg tggcgatgag gcaattcctg
    48901 atcaaaagga aagttcaatc aactttgacc ttagcgcagg gttatactac agtacggaat
    48961 acatgttttt tgggttctca gcgctacacc tgaacgaaac caaaactaat aaggcagcat
    49021 tacctgcacg gtatacccgt cagtactacc taactggtgg atatattgtt aattttccaa
    49081 accccacatg gcagtttgag ccttcagtaa ttgttcacag cgatttaaaa tcaagtaaat
    49141 tatcgctaaa tacaattgtt cggtacaata aaaaggtatg gggtggcgtt tcttaccgtg
    49201 tgggcgaggc gattactggc ttggttggtt ttgaactttt taatggtatt aagtttgggt
    49261 atgcttatga atttagtacc agtaaaatta gtaagtacaa caacggttct cacgagtttt
    49321 acatggggta taacttccag ctaaataagg aaaagccacc ccaacagtat aaaagtttga
    49381 gatttttgta acatattttt acaattgaac gaaaaaaatt acgttatttg cttaataaac
    49441 gatgccttat gaaaaaattt ctctccatag gtattttggc gtccctactt tatggttgtg
    49501 gacctggtaa tactggcgag ttaacgggtg ttccaggacg tagaagtttc agcgagccaa
    49561 caccgctggg aatggtgttc attcccatgg gttcatttac tctgggtagc aatgatcagg
    49621 atgttgcctg ggcaattaat gctcccacta aaacggtttc ggttgatgct ttctggatgg
    49681 atcaaactga aattactaat aaccagtacc ggcagtttgt ttactatgtt cgcgactcca
    49741 ttgcacgtcg cctacttggt gaaataaatg atgagttcct aatcacagag gaccagttcg
    49801 gtaaccctct ggatcctccc gttctcaact gggaagttcc aattgatatg gaagatgagg
    49861 agcaacgcga agccctcaat gccctatact attccaagga ggatgaattc tttggacgga
    49921 aagaggttga tacccgtaaa ctattctttg agtattactg gattgatatt aagcaagcag
    49981 ctcaaaaacg caaccgctat aactacgaaa ccctcagtta cgaccagggc gaggttataa
    50041 acagcctagg agagaaggtt aaggttgata accgccagag ctttatcatg cgcgataagg
    50101 taaacgttta ccccgatact ctttgttggg tagccgattt tacctatgcg ttcaatgaac
    50161 cttacaccaa tagttacttc tggcatcctg cattcgacaa ttaccctgtt gttggcgtta
    50221 catggaaaca ggcaacagcc ttctgtattt ggagaaccca aacactcaat aagtttcttg
    50281 cccctgaagg tattaccatt caagattacc gcttacctac cgaagctgag tgggagtatg
    50341 ctgcccgtgg ttatttgaac cacagcatgt atccttgggg cggcccttac acccgtaaca
    50401 tggagggatg cttcctggcc aacttcaaac ctctccgtgg taactatgtt gacgacggtg
    50461 gtttaatacc aatccccgtt ggcacttatg aaccaaatga ttatggacta tacgatatgg
    50521 ctggaaacgt agctgagtgg acagccaacg cctacgatga aagtgcatac tcttttatgc
    50581 acgatatgaa tcccgattat aagtacaatt caaaaccatc cgacttacca gcgctaaagc
    50641 gtaaggtggt tcgtggcggt tcgtggaaag atattagctt cttccttcag gttggttcac
    50701 gtacctacga gtatcaggac tcagccaagt cttatattgg tttccgttgc gttcgttcat
    50761 acctcggagc taagtagaag tgatgaattt tactaattga aaacattaca gttatgaaga
    50821 tcagcataga agaaattgta acatccaaga aatggaaaac cttcatgaag tacctttacg
    50881 gatggggtgc ttcagtggta attcttggtg cacttttcaa aattcttcac ctgaaaggtg
    50941 ctggtaccat gcttttccta ggtatgggta cggaatcaat aatattcttc ttctccgctt
    51001 ttgagccaat tcacgaagag gtagactgga ctctagtata tcctgaatta accggaatgt
    51061 ccgatgaaga ggaaatttca ggataccgcc gctccaggaa ccaaggtctt tctgctgagg
    51121 atgttcaaca aattattact tctgtgctgg catccttgcc cgcagggtct gctggagttg
    51181 ctgcagccgc tcctgcttcc gcagcaccgg cttcacaacc tgctgctgta ggagttcccg
    51241 gtgccctgat ctttaccgag aagttcaaca agatgcttga aaacgcagag attagtcctg
    51301 cccttttcga taaggtcagc gctgggctta acaagctggg tgatgcatcg agcaaaattg
    51361 ccgacattac caattcagca acagttgtaa aggagttcaa cgagaaattg acaaaagcat
    51421 cggagagtgt tggtactttt acccaaaatt actctcagag cggacaagca ctcaatgagt
    51481 caataaatgt tttaaaccaa aacatacagc aggctgcagg tgtaatgtcc gaaagtggca
    51541 aaaacttcat ggatggtgtt aaccaatcag ttaagaacct ggaggccgaa cttaccaatg
    51601 ctggacaaaa ggtaagcgaa cgaatagttg gttctgccga atcggttgcc gataatctaa
    51661 aagtctcagc cgatggactt gcaaacacct acaagcaaac agcgtcgcag gttgctacaa
    51721 cctaccagca gttagctgaa gctatgcagc ttaacggaaa tgttattacc aacggaagta
    51781 caagctataa ggaacagctt gagcggttga acaagaatat ggctgcactg aatgcagcac
    51841 atgaacttca tctgcaggaa accagccaaa ggcttaagga agccgaaaag gtttaccagg
    51901 gggttgatgg aatggttaag aaacttaact tcactattga agaaaccgag aagttcaggt
    51961 tggcagttga gaagttgaat agcaatgtgg ctgctctcaa taatgtttac ggcaacatgc
    52021 ttacggcaat caatgcccta tcaaacggaa agtagcagtt ttgcatatta cagcttaaga
    52081 tagctttatg gcacacggaa aagagactcc caggcaaaag atgatcggca tgatgtacct
    52141 cgtgctgaca gctatgcttg cgcttaatgt atcgtctgag gttcttgaag cttttgtttt
    52201 agttgacaat ggcctatcaa gaactacaga aagttaccgg cttaaaaaca agaaacttta
    52261 cgatgagttt gctgctgctt acagcataaa tcctgtaaag gttgggccct ataaacaaaa
    52321 agccgacgaa attcaaaaga aagccactga aattctacag tacgttcagg accttaaaat
    52381 agaggtggta aaagcatcgg agggtgagga aaccccagca atccagaatg gtgaaattat
    52441 tgctgaactc attcagggta aaagcgacct cgacaaagct gctaccatcc tgatagggtt
    52501 tgagggtaat ggcaaggcaa aggtcttaaa acaaaaatta gaagaattca gggagtatat
    52561 agttagcata attgacaagg ataaggctcc ggaacttgtt gaatcaatca agaaaattct
    52621 taatactgac gatcccccag taaaggaaga tggcacacac aacacctggg agagcgcacg
    52681 atttgagcat attcctttaa ttgctgtaat accccagctt acaaaggttc aggttgatat
    52741 tcttaacgtt gaagctgaaa ttattaacta tctgatgggt agcattggtg cggcagagtt
    52801 taaatttaat aagctttcag ccacagtaat ccctaactct aacattattt tccagggtag
    52861 cgagttccgt gcagagattt tccttgccgc ttccgatacc actcaggtac ccaaagttta
    52921 cctttgtcgt tacgactctg ttttcaatag cgctatcaat ggatatgatt accgtcctat
    52981 tggaaacact gaggaagtac cagtaaacaa aactggtaga gccgtattaa cccgtaaagc
    53041 ttcatcaact ggtgttgtaa actggcaagg tttaattgag atgaaagcac cagatggaac
    53101 aattgttcgc cgtccattca agcattccta cacagtaatg ccacccaacg tggttattgc
    53161 tccaaccaag atgaatgtgc tttacctggg tgtagaaaac cccatagata tttcagttcc
    53221 tggatttgcc cccgataagg tatcggcaac cataagccgt ggaagcatta aacccgttgg
    53281 tatgggtagc tacatagccc aaccaggtgc tggtgctcct accgtagaaa ttactgtttt
    53341 tgctgaaact gaaggaaaac gtaaggtttc catgggaacc aaaaccttta gggtaaagag
    53401 ggttccagca ccaattgcaa aagttattgg tgtttcagga aaaactgtta gtagaagtga
    53461 gttagcagca gctccaggtg ttatggctga aatgcctcag gactttgatt tcgaccttaa
    53521 gtttaaagta gttgggttta agatttcagc tacagttggg gggtttttgg aggagaaggc
    53581 atcgacaagt gctcgtatta ccgatgagca aaaaataatg attcgatcac tttcctctgg
    53641 cactcaggtt gcaattactg agataaaagc cgtaggccca agcggagatg tggttgattt
    53701 gaacgattta gttttaaaga ttagataata tactgccatg aaaaggattt tattgttctc
    53761 agtcgtgata ctccttgcat cctctgcatt gagagcacag gatagaaagg aaagtttgga
    53821 acgcgatgga atttataccc gtgaaactac accggcccgt gttccaatac cctatcaatt
    53881 tgtaagagaa gcggatgtta tatggtcaaa gcgggtgtgg agagttattg accttcggca
    53941 gaaaatgaac taccctcttt actacccaac taaattaatg caggataggg aaagtttagt
    54001 tcaacgactg gttagagcaa ttaagtacaa cgagattaat gcatataagg atgaggaatt
    54061 tactgaacgt ttatcatacc aacaggttat agaagagagg cttggcgcaa aagataaagt
    54121 tgaagatagt cttgaccttg aaggaaatat agtttatggt caagatggga taccattaaa
    54181 aaaaataata ccaggggaaa taaattggag ggatataaaa gaactattgg taaaggaaga
    54241 ctggttcttt gataagcagc gctcggttat ggaggttcgg attattggaa tttgtcccat
    54301 tcttcatcgt acggattacc gtaaaacagg aagtgatgag gaacaagaag gaaccctaag
    54361 cagaattcca acgttttgga tttatttccc tgaagccaga agtgtattgg ccaataccga
    54421 tgcctataac aacttcaacg atgctcagcg aattacctac gacgaccttt tctggaaacg
    54481 taattttgat tcatacatta tcagaatatc gaacgtatgg gataacaggg caattgatga
    54541 gtacactttc actggtttgc aaacattact tgagtccgaa aagattaaaa cagagctctt
    54601 caactttgag cacgatttgt gggagtacta gggatattat ctaaaatact taaggccgtg
    54661 ggtttaccct acggcctttt tcgtatgcaa acaagtattg gtttaagcaa aatccttatt
    54721 gttcattaaa acatcgttca caaaatttcg tatctgctgc tcatcctgct ttttgcatat
    54781 aagcagaacg tccttggtat caaccacaat atagtcgtcc aaaccctgaa taactgcaag
    54841 cttatttttg gggatattga aaatggaatt gctggtttgg taaagcataa gctgctgggc
    54901 aatggctgca tttccatttg ggtcatgggg taggtgagtg tatagagagc cccaggttcc
    54961 caaatcggac catccgaact cggaacaaaa aacgtaaaca ttatcagctt tttccataac
    55021 tccatagtca atggaaatat tcctgcactc ggagtatgct gtagcaattg tttcggtttc
    55081 tttttcggta ccataaatgg gtagtaactc cttaaataag ctgtttacct caggtaggtg
    55141 gctttcgagt gccgctgaga tagaggcaat gctccaaata aacaagcctg aattccagta
    55201 aaactcacca ctttccaaaa acttttgtgc aagctccagg tttggtttct cggtaaatgt
    55261 tttgaccttg aatagcgagg gaacttcctt tacaggttta cctatctgaa tatatccgta
    55321 accggtttca ggcctacttg gcctaatacc cagagtaatc agggcattac tgttcgaggc
    55381 aaattcagcg gcttttaaaa tggtatcgag aaaaatatct tcattgagaa ttagatgatc
    55441 gctaggagct acaatggcta tggcatttgg gttttgttgc ttaatcttgt atgtggcata
    55501 tgcaatgcag ggtgctgtgt ttcttctaag gggttcaagc aaaacctgat caggtgatag
    55561 attgggtatt tgctcgttaa ccaatgaact gtatgcacta ctggtaacaa tcagtatatt
    55621 ttcgcggggg caaacccggc tcatcctatc aaaagtctgc tgtaggagcg atttcccagt
    55681 gcctagtata tcgataaact gttttggttt atcaactcgg ctaagcggcc aaaagcgact
    55741 tccaatgcca cctgccatga taacacagta taggtttttc ctttcaatca ttgtttttac
    55801 aaattaaatt gcaaaagtag ttcatttttt ggcacaaaaa aactgctaag aatttgaatg
    55861 caacccgata tggcagagag tcattaacgt aatccgtaaa gtttaacaaa tattttctgc
    55921 gttgcttaat atttaagaaa atcatttgtt aaatggttca aatcacaata ttttgagtta
    55981 ttgcctgaaa tattagttac taaatgctac ttatttttgg atataccatt aactttgttg
    56041 gaggagtttc agtttgttta aataaaattt ccacctatga aattgctttt tgcaattgat
    56101 atgtttgggg agtatatact ccttctgaaa aaggtttttt caaaacccga aaaacgtaga
    56161 gtttaccttc agcaaactat taaggaaatt gataagctag ggctacagtc aattggtata
    56221 gttacaatta tttcggtttt tatgggtgct gtaattaccc ttcaaaccgc ctacaacatg
    56281 gagaaccctt ttattcctaa ttaccttata ggacttggta cccgcgatag catgttgctt
    56341 gagttctcat caacaattat tgggttgata ctagccggaa aggttggttc taatattgct
    56401 tcggaaatag gaacaatgaa ggttacagag cagattgatg ccctggagat tatgggtatc
    56461 aactcagcaa gctttcttat acttcctaag attattgcca caattctatt caaccccttc
    56521 cttaccgttt taagtatgat agttgggatt gccggtggat ggttggtagg ggtatttacg
    56581 ggcgttgttt caacccagga atatatcgat ggggttcagt acgcctttat tccgtattac
    56641 atcacctatt cgcttattaa aacagtagta tttgcttttc tgattaccac gatctctgcc
    56701 ttttatggct atagggttca aggcggctcg ctggaggtag gtagggcaag tacccaggct
    56761 gtagtaatga gtagtatttt tattcttctg tttaacctaa ttctaaccca actcttactc
    56821 tcgtgataac cgttaaaaat cttaataaat cgttcgatgg gcgtttggtt ttagaaggta
    56881 taaacgccac ctttgagaaa gggaaaacca acctgattat tggtcagagc ggatcgggta
    56941 aaaccgtttt gctaaaatgt attgttgggc tgcatgaggt tgattctggc gaaatctact
    57001 ataacgacac ccgattcgac aatctgaatt ttagagacag gaaaaagata cgccaaaaaa
    57061 ttggtatgct ttttcagggt tcagcccttt tcgattccgc tactgtggag gaaaatgtta
    57121 tgttcccctt ggacatgttc acaaccatga gccacgagga aaagctcgat agggtaaact
    57181 tttgccttaa ccgtgttaat attatcaatt caaatcacct ttaccctgct gagattagcg
    57241 gaggaatgaa gaaacgagtt gctattgctc gagccattgc tctcaaccct gaatttcttt
    57301 tttgcgacga gcctaactca ggtttagatc ctaaaacagc cgtggttatc gatcagctca
    57361 taaaggaaat tactgaggag tttaatatta ctaccatcat aaacacccac gatatgaact
    57421 cggttatggg tattggcgat aaagtaattt ttatccacaa tgggaaaaag tggtgggagg
    57481 gtagcaagga aaatattctt gatagcgata ataaggagct gaacgatttt gtcttttcca
    57541 cagagctgac ccgaaaaatc aaagggttat agcttaaccg tgcattttta tccagcgtaa
    57601 cttttcgggg tcaacaattt caatggtggt tccctcaata cgaataatgt tatcgcgcct
    57661 aaactccgac agaatcctca caacattctc aacccgcata tcaattagtt cagcaatctc
    57721 gcgtcgggag atgggaaggc taaatttatg cgatttgtaa acctcattcc caaagtagca
    57781 aataataaag gcaaccctgc cacggagctg acgaagctcc aaatccaatc gggttcccat
    57841 catcagatcg gccacctgcg atatcttttc cagaagtgtt tttgcaaaat cgccattggt
    57901 gctaattaag ttaatgacca ggttgtagtc aacacagcaa tactcgcaat cctcaatggc
    57961 tattatgttg tactggtagt tttttgatga aaaaatgcta agtaagccca caaagtcgtt
    58021 tggagtggca atacctatga tttgctcacg tccatcgggg tttacacggg ttagttttgc
    58081 tagccctaca cgcaagaaaa tgaatccttt tatcgcgcta tcctgcttgg ctattgtttc
    58141 gccctttttg gcaaagtgaa aggttttatt tgcggtaagc tgattgagct gctcatcggt
    58201 cagttttgaa aaaaccgaag aacggaaacc acattcagca catatattgc ccggacaaaa
    58261 attttggctc atatctacat atattgatat atatcagaat tgatttttat caacagctgt
    58321 aaatctaaaa cttttgttac tattaacgta ctttgaattg ataaaatttt aacaattaag
    58381 caaaatttaa taattaacat atgaagaaga ttgtgattct aggagcaggt acaggtggaa
    58441 ccattatggc caataagctc cgtaaggttt tggaacgcga tgaatggagt attactgtgg
    58501 tggataagga cccgaatcat tactaccagc ccggtttcct gtttatacct tttgggatat
    58561 acagcaagca cgacgtggtt aaacctaagg ctagctttat tcctgccggc gtaaagttta
    58621 ttgtaaacga tattgaccgt gttgaacccg acaagaacac agtttaccta accaacgggc
    58681 aaattctgga gtacgatttt ttaatagttg ccacaggtac gcatattaat cccgcagaga
    58741 cacccggttt gcttggtagg gaatggcgta agagtatttt cgacttttat acccacgagg
    58801 gagcagttgc cttgagcaca ttcctaaaaa catgggaagg tggcgatatg gtaatcaata
    58861 ttgccgaatt gccctttaag tgtccggtgg caccgcttga gtttgcattc cttgccgatg
    58921 agttctttgt gaagaaagga attcgcgata aggtaaacat ttactatacc acaccccttt
    58981 caggtgcttt taccaagcct aaatcttcaa aaatgcttgg ggaactcctt aaggagaaaa
    59041 acattcaaat catacccgac ttttaccttg aaagggttga tgatgaaaac aaaaagatta
    59101 tttcatacga tggccgtgag gttaaatttg acctgctaac cataatccca gttaacatgg
    59161 gtgccgattg ggtggctcgc agcggtatgg gcgatgacat gaactacatt gccaccgaca
    59221 agtacaccct acgttccgag aaatgggaga atatttttgt gcttggcgat gccagcaaca
    59281 tcccaacatc aaaggccgga tcagtggttc actttgcttc ggatgtgcta ttcgagaatc
    59341 ttatggctgc cattgagggt cgtcccctaa ttgccaagtt cgatggtcac tccaactgtt
    59401 acatcgaaac tggattcggt aagggttcgc tgatcgactt taactacgaa acagagccgc
    59461 ttcccggaac atacccctta cccggaattg gcccgtttgg cttacttaaa aacacccgga
    59521 ttaatcatta cggaaaggtt atgttccgtt ggatatactg gcatctgctc ttgaaaggta
    59581 aagagctacc gcttgaatcg caaatgacta tggctggtaa ggaactttaa aactgtttgg
    59641 ccatggagaa taatttgcaa caacaaattg atgaccttaa ccgcaagatg gatcttgtgc
    59701 tggagttgct ggccaagcag cagcagcgca gcgaggtggt tgaggatttg gtggaggatc
    59761 tgaacattgt agtaaaggac gctttccaga ccgctgtgga tgagctggct gttcagaata
    59821 ttaagattga tggggaagat gttcggtatt tggttttcaa attgcttcgc aatgtgaaga
    59881 cctttggcga gctcatggat atgcttgaga gcttaatgga tttcctccac gatattgggc
    59941 ctgtggttca cgatgccggt attgccttta caaaggcgct ggatgaccta gacaggaagg
    60001 gatacttcga ctactttaga cagttaggcc tactcgctgc cgagttcaag gagcatgtta
    60061 ccgaggagga cattattcgc cttcgtgaga gcatgccagc agtagggttg atgttacgta
    60121 gtttaactca acccaaggta atgaatgcct tggctgaggt aacccaaacc ctttccagca
    60181 tggaaatgga cgaaaagatt gacaataaat cgttgattgg cattgcccgt caactaagca
    60241 aacctgaagt tcgtcgtact ctctcgttta tgttaagggt aatgggcgaa cttgggaaaa
    60301 ctaaaaaaat taattaatca ccaaaaacaa ataaagctat ggcagaaaaa gtttatgcag
    60361 gcaaatccgt tagcgtaacc gacgatggtt acctgaccga tccaaatcaa tggacaaaag
    60421 aggttgctat tgaaattgca aaggaggaag gtatagaact taccgataag cactttgctg
    60481 taattgagtt cattagggag aaaaccctca agggcgaagc tctcaccatt cgcacgattg
    60541 gcaaatcggg gttggttgat acaaaaggtt tttatgaact ctttccaggt gcacccctga
    60601 aaaaggccag ccgtattgca ggaattgcaa aaccatctag ttgtgtataa ccctaaaaac
    60661 agttaaatat ggcaaatgca caggatcacc ccattaagaa ggtgatgata atatgcgcca
    60721 aggcaaacct ggaggatgtt tatgcagcct tggttatggc aaacggcgca gttatggagg
    60781 gtattgaggc taaggtgttt ttcacctttt tcggtctcga tgctatcacc aaaaagcaaa
    60841 tgaagaggtt acacaccgct actgtgggta atccgggttt acgaatgccc ggcggtttac
    60901 ccttccctac tttactggga atgcttcccg gagttgaagc tggtgtttcg gctatgatga
    60961 agagccaaat ggaaaaactg gacatccctc cagtagatga gtttcttgaa ctcattaccg
    61021 ctggcggtgg cgagatatat gcctgtaaac ttgctgttga aatgtttggt cttaagaagg
    61081 acgatttcag cgagcatgtt aaggatataa ttaccattgg ccaattctac gagttggcag
    61141 ctggcgaagg aacgcaaata atttttgtgt aagaatatct gattccatca aaagttaaga
    61201 gcctccaaca agggggctct tatgctatat cctaaaacca attattgtaa tatcatcggt
    61261 ttgctggcta tcgcctcgcc agtcatcaaa gcattgttca atctttttgt tgattgtatc
    61321 caagttgcca ttggcatttt ccaggagtat gctttcaaac tgtttacgac caaatcgctt
    61381 cttttcggat tttgatagct gatcgaaaag gccatcggtg gtaagaacta aaatatcgcc
    61441 actttctaat tttaattcaa caagtggaat atttggggtg aaccatcggt ttcctactgc
    61501 tttgcccccc gacttgtaaa ttttcagctc aggactttcg aaattcctct gaatgtgata
    61561 cagggggtta tgagtgctag cgaagagtgc ggttttattt attctattta catagcacac
    61621 agctatatcc atttcaacgg gagcgctgga agcgtttagc tgcagctggg caccaatggt
    61681 ctggttaaag tggttgtggg tttcaataat cagttcaacc ggattattga ttttatagtg
    61741 ggtataaacc tcgtttaaaa gtgttatatc catcatgctt acaaacccac catgtactcc
    61801 atgaccagta caatcggcaa cagctataat gaattcatcg gagtttcctt ttagccagta
    61861 aaaatcgccg cttaccacat ccttaggttt ataaaagatt aggtgttcac ctaaaatggt
    61921 tgacaaaatc tcggggcttg gcattaagtt ggattgcagt aactttgaaa actcaagact
    61981 ctcgtttatc tggtatgttt ttagttttaa attgttgctt tgctgtttaa tcagatcggc
    62041 ctgcgctttt agctcttcgt tttgtgctaa aatcgccttt gttttctcca ttacggcttg
    62101 ctcaagttcg tcctttttcc ttttcaccct ttgaacccgg agtatttcat gaatgtaaac
    62161 cgataaggct aatagtaata aaaatatcag gaagaaccat ggtttgaggt ataaaggcat
    62221 tccaacaacc acattgattg taaaaacgtt ttttgattta gtgccgtcgg gccgggtata
    62281 gtaaaccttg aaggtgtatt taccataggg gagatttgtg tattcagcca aaggcgaatg
    62341 agttttcttg gctcttgttg aatcctcaaa gccctgaagc cagtatgtgt actctaatgc
    62401 tccgggtgat gtgtagtccg atgcagtaaa atggatttta agccatttgt ggtttgcttt
    62461 tacaaaaatt tttgaattgt tagcaggaaa gcgagctaat gtatcgtagt cagtttcgca
    62521 cgataaaatt ttaatatcct gcatgggtac ttcgtttcct tgtacataag gattaaaaat
    62581 atacaggcca aagttaccgc tcactactaa cgagtcgaac ccccattgtt caatggcagt
    62641 aactaaaccc tcactgaaat taaggttttt acctgttatg tcgacccgtt tgagtttctt
    62701 gctaaaacgg gcaatgccct tatcggttcc aatccagtaa ttcccgagtc tatcctggta
    62761 aatggctgta acaactgatg atgggagccc atgtcgggtg tcaatcatct gaaaagcctt
    62821 gctttccggt tgaagaatgg ctaatccggc atcggttcca aagagcatat ttccattggc
    62881 atcttcaaat atacagtggg tcatgcttgt ggctgggtac gatgttgaat cgtagggtag
    62941 gtatgggaaa tgatcaacgg tatgtgttct tcggtcaatc ctatcgactc ctacaccatt
    63001 ccttgtgtat ccaacccaaa cgtaaccttt ggagtcctca tacatcctgt aaacaagggt
    63061 cgactgaagt gcgcttttgc ccggaacata gtagctctcg tatcgcttct gtttaggtag
    63121 atattttgat atacaaacat ccgatagaat ccaaacattg cccaatgcgt cggtagcaat
    63181 tgatttcact tgaaccgatg gcaagctatc cttattttgt ggatacgccc caatggtttc
    63241 aatgctattg gttttaagcg atagtctgaa caactcctca gagttggttg cccaaacatt
    63301 tccgctacta tcaattgtga agttggttat actctgttcg ctcaggtatc tttttactaa
    63361 tcctttgcca ttaaacatca aaagcccctt gttacttgct ccaaaagcaa atcctttata
    63421 gaaagtaagc gcctgaacat cggcatcgaa tagtttggta agagttttag tatgttctgt
    63481 cattacatat acaccttccg atgtgccaag aaaaattgta tcgttgccat cggcaatcga
    63541 ttttattagc ggaaattgct tgatagaggt taactggtca aatctccctc gctcttggtt
    63601 ccaccggaat aatacactat cggttagggc atataaatac ttatcgacaa aaatatttgt
    63661 aaagttgata ttagtggaaa gagggggctc aattttacgg gtaggtaagc taaaagggta
    63721 tattttatta tcgttaccta aaatcattaa attgctattg aataatagca ttttctttgc
    63781 ctgaatattg aacgtaactt gcttttgctt aggaatacca atatctagat tagtaaagtt
    63841 actgggaact atgttgagtg tatcggagtt taagacattt aatagtgttg caaactcaaa
    63901 taaaatccat ccgttgttag aagttgttat tttattgata taacctgaaa cgttagagca
    63961 ttggttttca aaacaaccaa aaattctaga agtaacgttt tcggttcttg ggtcaaattc
    64021 attaactctt cctccccatg gtgaaaaaag cgctttgcca tcggatgttg ttattacgtt
    64081 ggagtaccag tagtaaccat ctggattgta tcgtttaaac cgtttttttt cgggccaaaa
    64141 ccgatttaac gccccaccat atccattcca aagggtaaca agcattgagt tgtcagttgt
    64201 cctggcaatg gatgtaaccc agtttgatgc aaggctatcg gggtggttaa aatacccgaa
    64261 tcgcgtaaca tttttggtgt taaaatcaat gaatacaagt cccttgtttt gggtacccaa
    64321 ccataaccct tttgggtagt ctggaaacat acaggtaatt tggtttgatg gcaggctgtt
    64381 aacgtcggca atttttcgga gatgtgtcga ttttcgggca aaggttgtta gcagcgataa
    64441 accattgttg gtgccaaacc atgtgttccc ctgattatcg cggtaaatta ccaggatcat
    64501 gttacctgcc agcgattgcg gattaccctc catcctaatg tagcgggtag ccgtgtttgt
    64561 cgatgggttg taaagtacaa ccccaaagtg gtagccaccc atccatacct ttccatcgcc
    64621 gttcgggcaa atggtataaa ccgatggtag gggaagtggg gttcgctcca tggttttctt
    64681 gttgattcgg tatgcattat caacatagtt tgctacccag atataatcgg agccatcgtc
    64741 gtaaaaattt gcagttcctg caatagtaat tatttcgtta tttataatgg tttcaacact
    64801 agggtaagta ttccatttat cgggatttcg ggttaaccag gtaagatgag cagaaccacc
    64861 tcccagcatt acaccctcgc caagttcggt tatggcgtgg aagtagtata tttcattgta
    64921 gacatattcg tgctgaaagg tttcaacagt tttagcgtca aagtcaatgc aattgagcgt
    64981 tccattgtgc ctatctaccc ataaccttcc tttactatcc acgtagatat ctcttaccca
    65041 acggtgtccc tgcagcggtt ccccaaatag ggaatcaacc cgttcaaagg tgtcggattc
    65101 tggatggtaa acacatagtc cgcctttagt gccaacccaa aggtttttct tcttatccac
    65161 agcaagtgaa cttacaacgc tgtgcggtaa ttgacctttt gtttttccac cccggtaggt
    65221 tttaaaattg ttaccatcaa atcgcattag tccatcctgt gtggcaaacc acatcagccc
    65281 cattccgtcc tggataatat cagtaaattc gtacgagcat attccatcgg actggtagta
    65341 gttgataaaa ctggtgtagt agtctgtctc taccttgctg aattccgatt gtgagtaaag
    65401 cgttttaaat tgaaagagag taaaaaataa ggctaacaaa aaattcctac ctaaagtcat
    65461 agatctactt cattagggtt agtaaaattc ttgtgagcaa gctatgggct atgccaatgg
    65521 ccccctcgga gggattaaag ttgggtctat gaagctgtgg attatcagaa cctggaaagc
    65581 cagcaccaag cctaatgaat accgatggtg taagggtgct gaagtatgca aaatcgtcgg
    65641 tggtcatgcg gggctcaagg tcaaccactt tatcatttcc aagcagttca gtagcagtct
    65701 gcttaacaag ttgtgttagt tcggggttgt tgaatattga tggatagccc ttacggattt
    65761 caacctcgca ctttgcacca tgtgatgctg ctattccggt tgcggtttgt tgtataagtt
    65821 tatgagcctc ctcgcgccat tgctcatcca ttgtgcggaa ggttccggaa atgctaacat
    65881 tgtcgggtat aatatttgta gcgccattgg cctcaatacg gccaaacgat agcacggtgg
    65941 gtattagcgt tggagctttt cggctaacca gtgtttgcaa gccaaggaca atttctgcag
    66001 cacatgtaat tgggtcgata agcaggtggg gtagggcagc atgccctccc ttccctttta
    66061 cagtaatgta cacctcgtcg cccgaggcca tgtattttcc ggggcaaaca ccaattttcc
    66121 caacttcaag ttctggaagt acatgaacgc cgaacatggc atcgggttta agttcattgt
    66181 atagcttagt ttgaattatg gcttgcgctc caccgggcaa aacctcctcg cccgattcaa
    66241 atattgcaac aaaacgtcct tttaatttat ccctttgcct ataaaggctt aacgtaacac
    66301 ctatggcaat agccatatgc atatcgtggc cgcaagcatg cattacgttg gggttaaccg
    66361 aagcaaaagc aacaccgctg gtctctgtaa ttggtagcgc atcgagttcc gcccttagcc
    66421 cgtatgtttt cccaggcaat ttgccttcaa ctattgctgc aaagctgtga tgtgtaccaa
    66481 aatcctcaac cctcactcct tcggttgcaa aaatctgttt gatgtagctt gatgttttta
    66541 cctcctgaaa cgagagttcg gggtgtttgt gaaggtggcg gcggtattcc accagtatgt
    66601 tttcagtatc gctttttgaa atcatcgccc taaaatcatc ttaataccaa ctaaaacaat
    66661 gaaaaacccg aaaatcttcc ggagtgtttc ggccgggatg ttaatggata cctttgagcc
    66721 aaagtagctg ccaattacaa cacctaccat tagaatcagt gcaagtttcc aatcgataaa
    66781 tccagcacgg tagtagttta caacagccaa aattcccacg ggaaagagta ataccataag
    66841 gctggtgccc tgcgcctcgt gctgggaaaa atttagcaaa tagattaaag ctggaacaat
    66901 aattatccct ccgccaaggc ctaaagcacc acccacaaaa ccggcggtta gcccaataag
    66961 caaaagcaaa acaaggtgtg caatactcat tttaaaacat ttttaacaaa tttagtaatg
    67021 tttttggaat atatgttgat gataaaacat ttacatttaa ggcgatttta gaaacccgtt
    67081 ttataacttt gtacgtagaa aattgtaaac gtttcaaaca tattgatcat gaaaaagata
    67141 ttgacaattt cagttttagc tgcttttgta ttttcagttg cagcgcagaa tgcagttcca
    67201 aaaatcaact ttaaaacaag tacctacgat tacggtaaaa taaaggaaga tgatggggta
    67261 gccaactatt cctttgagtt taccaataca ggtaatgccc cactaatcat tcagcgtgtg
    67321 gctacatcgt gtggatgcac aacccctgaa tggcctaagg aacccatagc acccgggaag
    67381 ggaggcaaaa taactgtagg atacaatcca gccggacgcc cagggccatt tgcaaaaacc
    67441 gtaaccatat actccaatgc cgaaacgcca accgtaattc tccagattaa gggcgaggtt
    67501 attccgcatg ttaaaaccat tgacgaaatc tatgtaaccc ctattggcga cgaacttaag
    67561 ttcgaaaaaa ttcattttgc tttgggtaga atattcttag gcagtaccta taacgatacc
    67621 attaggttca ttaataaatc aagtgcacct gtaacagtta aggccaacac acagggaatg
    67681 aactttatgc aggtgaaagt ggtacccgag gtggttaaac caaaccagtt tggcgctatt
    67741 gtgctcacct ataaccccga taagcgcgaa gagtggggct ttgttgttga taggttcacc
    67801 atttctgtta acgataaagc tatgaacacc agcccaataa ccattaccgc aagcattgag
    67861 gaggattttt caaagcttac gcagaaggat ttggaaaatg cgcccaaggc tgagttcaat
    67921 accctacagt acgattttgg aacggttaaa tcgggcgacc ccgtaaacta cgattttgtt
    67981 ctcaagaata caggtaaaac cgatttaatt atccgcaaga ttaaggcaag ctgtggttgt
    68041 acaactgcaa ccccatccaa gagtgttctt aagcctggtg aatccactga gatatcagct
    68101 tcgttccgaa cagctggata ttccggccga cagggtaaaa ccattacagt tattaccaac
    68161 gatccccgta actcaacatt aatactccgc ctaacaggaa atgttgataa gatagaatag
    68221 caataaagcc ggtgttaagc cggctttttt ttgggggaga tgcagaggtt tgtttatggt
    68281 ttacc
//
LOCUS       DAOSAL010000005        64001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_361_length_64001_cov_50.624073, whole genome shotgun sequence.
ACCESSION   DAOSAL010000005 DAOSAL010000000
VERSION     DAOSAL010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 64001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 64001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64001
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_361_length_64001_cov_50.624073"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            513..857
                     /locus_tag="PK049_01435"
     CDS             513..857
                     /locus_tag="PK049_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60247.1"
                     /translation="MKIGELAQLVDGNVVCGHAMLDREIEYAFASDLMSDVLTVRADK
                     LLLITGLANMQAVRTAEMADIPAIVFVRNKTISEDMIQLAEDNGIVLIESPFTMFRAS
                     GILYSKGVKPVY"
     gene            864..1280
                     /locus_tag="PK049_01440"
     CDS             864..1280
                     /locus_tag="PK049_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOK60248.1"
                     /translation="MHFEYRVQGGDFVRAGQASSQVKKILKQLNVSPNIIKRVVVALY
                     EGEVNIVAHAFQGKIYVDIETDKIRVVLDDVGPGIPDIDLAMKPGYSTASPIVREMGF
                     GAGMGLPNIKENTDKMKIASVVGVGTRLEFEVNLKE"
     gene            1293..2660
                     /locus_tag="PK049_01445"
     CDS             1293..2660
                     /locus_tag="PK049_01445"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOK60249.1"
                     /translation="MADLEFYHALKILPDVCIGCSHCMRACPTEALRVVNGKAKLINN
                     RCIDCGECYRVCPTHAITVQQDDFSKIFSYKHRIALIPSVLLGQFSKEISHDDVAQAL
                     LSLGFTSIVEVEDSVEYLIGKLNSYIASTEVKPVISSFCPAIVRLIQVKFPSLVENIA
                     LIRPPLDLTATYIKTSLLEQGVPEKEIGIFYITPCAAKIVAVRHPEGEEKSEIDGIIN
                     MDFIYNRILREIKGKLPTGNEPKIISDLSPRSYSWALTNGEASCIDGRCLAIDGIWNV
                     IEFLEKVENDEIQDVDYLELRACDESCAGGILAPGNRFLAAELIRKNASKVSVWSINK
                     TNDKIVKYDACISGRTALSPLSPRSMLKLDEDMAKAMLKMQRVNQLMCFLPGIDCGVC
                     GAPSCIALAEDVAQGSASVSQCVFLQKIMEQNKLDPEHSFRIMKRIWGENRFRKDCDK
                     PIRNK"
     gene            2673..3011
                     /locus_tag="PK049_01450"
     CDS             2673..3011
                     /locus_tag="PK049_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005643247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine kinase"
                     /protein_id="HOK60250.1"
                     /translation="MTVKDIVDALNLKVYSGEAGLNKEVVGGYTSDLLSDVMGNAPAG
                     SVWITLQTHVNVMAVATLKDISAVILVKGLEPEANTMAQSNNEAIPVLGTNLQAFEIS
                     GQLYNLINKK"
     gene            3008..3748
                     /locus_tag="PK049_01455"
     CDS             3008..3748
                     /locus_tag="PK049_01455"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOK60251.1"
                     /translation="MIVKADLHIHSVISPCADLEMSPVNIVMRAAEMGLKLIAVTDHN
                     STLHGPLIRTLAEQHGIYCLYGAEVNTREEIHCLCLVDTEDQRQLLQAFIEQNITRIP
                     NNPDIFGYQVVVNEHDEIVQEIDYLLHAALAKSIDEIAEFVHGIGGVFIPAHVDRPKY
                     SVTSQLGFVPPSLSYDALEISRNTTVDSFKRNNPIKPNTQFIRNSDSHFLSQIGTSYT
                     EYNLENLSFKCFKDSILNTGSSTVLPVI"
     gene            3793..4347
                     /locus_tag="PK049_01460"
     CDS             3793..4347
                     /locus_tag="PK049_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008157727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOK60252.1"
                     /translation="MKDLSLHILDIVQNSVVAGAKVIQIILDINTANDYIELTVKDNG
                     KGMEQEFLKRVVDPFTTTRTTRKVGLGIPLLKQNAELTGGAFSIDSEPGVGTALKARF
                     VKSHIDCLPLGDLGGTIALLLCSYPDIEFKVSVVVDTTEFAVSTSELRQALDGVPLNE
                     PSVYPLVKEYVYSNLDELQITNLS"
     gene            4455..4853
                     /locus_tag="PK049_01465"
     CDS             4455..4853
                     /locus_tag="PK049_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOK60253.1"
                     /translation="MTKVKSLADLKKMKETLQAKIDLREKGEAAENLVQIKVAMATCG
                     IASGAKNVMDFLLEELEKRGINAVVTQTGCMGYCYAEPTIEVKLPNADPVVFGYVDTK
                     KADEIIEKYIKNGELVDGIIPVNYESIDNK"
     gene            4874..6667
                     /locus_tag="PK049_01470"
     CDS             4874..6667
                     /locus_tag="PK049_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010803102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOK60254.1"
                     /translation="MSKYKMHLLICGGTGCKASSSDAIAEKLKLELAANNLQDEVQVI
                     MTGCFGFCEKGPIVKVLPDNTFYTQVKPEDATEIVKEHVVKGRKVERLLYVDPTKKAA
                     IEDSKHMGFYRKQMRIALRNCGFINPENIDEYIAREGYQALGKVLTEYTPQQTIDIIK
                     KSGLRGRGGGGFPTGLKWEIASKNHADQKYVVCNADEGDPGAFMDRSILEGDPHSVLE
                     AMAICGYCIGATKGLIYIRAEYPLAIKRLKVAIDQAREYGLLGSEIFGTSFSFDIELR
                     YGAGAFVCGEETALIHSMEGLRGEPSNKPPFPAQSGYMGKPTNVNNVETFANVPPIIL
                     KGADWYSSIGTEKSKGTKVFALAGKINNVGLIEVPMGITLREVIFEIGGGIKNGKKFK
                     AVQTGGPSGGCLTEKHLDTPIDFDNLIAAGSMMGSGGMIVMDEDDCMVSVAKFYLDFT
                     VEESCGKCSPCRIGNKRLYELLERITKGEGTMEDLERLRNLSQVIKDTSLCGLGQTTP
                     NPVLSTLDNFYDEYVAHVVDKKCPSGQCKSLMQYVIDPEACVGCTACARNCPVNAISG
                     ERKKPHEINQSICIKCGACMEKCKFDAISIK"
     gene            6682..8457
                     /locus_tag="PK049_01475"
     CDS             6682..8457
                     /locus_tag="PK049_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOK60255.1"
                     /translation="METVKLTIDNKQVEVPKGTTIYKAARKLGIDIPVLCYMELHDLG
                     VENKPAGCRICVVEVEGRRNLAPSCATECAEGMVVKTHTTRVINARRTVMELILSDHP
                     KDCLTCPKSGRCDLQDMAIKLGIREIPGQEYAEMSTYKKDFSPSIIRDVDKCIMCRRC
                     ETMCNEVQTVGALSAVNRGFMAVVAPAFEQDLEKSPCTYCGQCVAVCPTGALTEVDHT
                     TKVIRAIADPTKTVLVQTAPAVRAALGEEFGMKPGTLVTGKMVAALRQIGFDYVFDTD
                     FAADLTIMEEGTELLDRITRFLNGDKSVKLPILTSCCPGWVNFFEHNFTDMLDVPSSA
                     KSPQQMFGAIAKSYFAEKLGKKREELVVVSVMPCLAKKYECQRDEFKVDGNQDVDFSI
                     STRELAHLIKEFNINFEELPDDDFDRPLGESTGAGVIFGATGGVIEAAVRTAYEAYTG
                     KPLPKIDFTELRGMEGIRSATIDFNGLPINIGIAHGLGNARKLLEDVRSGKANYHAIE
                     VMACPGGCIGGGGQPLHHGNSAIIKARMEAIYREDAGKPIRKSHENPYIVKLYEEFLG
                     KPMSEKAHHLLHTHYFSKKKDIYIK"
     gene            8471..8959
                     /gene="nuoE"
                     /locus_tag="PK049_01480"
     CDS             8471..8959
                     /gene="nuoE"
                     /locus_tag="PK049_01480"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005836594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOK60256.1"
                     /translation="MSHIKLELKPEQVAKIKEICKSFNNDPGELINVLHKTQETFGYL
                     PAEVQEVVAAELNISVAKVYGVVTFYSFFTMIPKGKHPISICTGTACYVRGAEKVLDE
                     FKRVLNVKVGETTPDGKFSLNCLRCVGACGLAPVVLIGEKVYGRVSPDGVKDIIKEYE
                     DQ"
     gene            9121..11622
                     /locus_tag="PK049_01485"
     CDS             9121..11622
                     /locus_tag="PK049_01485"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOK60257.1"
                     /translation="MMKKVLLLWAIAVVTVTSMAQDRMQGRSGQNFDMSNFNGVITGK
                     VIDSLDRNGVEFANIALYKQKDSSLVTGTLTDASGNFMMEKLMPGRYYVDVKFIGYQS
                     RRISGIMVSPRSPKVDLGTFSLLAASENIEAVVVTGEKKMLLHNLDKKVFNVDKDIAV
                     EGGTAADVLQNIPSVEVDTDGNVSLRGSSNVNILIDGRPSMLNSMEELPAQMIDRVEV
                     ITNPSAKYDPDGITGIINIVLKKRKEPGYNGMVSLNAGTGNKYNATVNMNWRQDKVNV
                     FANYSFRRGQMDRFSIGDRVTLFNSGADSSFLSQNSEGQSNMFFHNIRGGADYFIDSR
                     TTLSFTGNLNFRSFDMDNNVLSNSYSNFNSNTLQNTRISLNSNDGLGHEYSLNFKRTY
                     DTPGKEWTADAFFSRNTNNNLNNINQQEIFNTVPTTALERAETDGWMNTFTFQTDYVT
                     PFGNGGRIETGIKAQIRRSDADYVYKIFNSLGSTWDFDANRSNHFVYNEEIYSAYGIY
                     SNTFANGKFSYQLGLRVEDQHSKSDQRTTNEVADVNRLNLFPSAHIRWEPNPINSLQL
                     SYSRRVNRPSVMVLNPFLNTSDNFNWSKGNPYLEPEFTSSIDLSHNLNFPRTKITTSV
                     YYRDTRNGFSRRMIVIDTVATQPTLTTFINLSHYENIGAEAVVTQNIAKWWRINASYS
                     YYYSKLFGDVVSGANEGSSWNAKLASFFTIGKNVDIQLTGNYRAPSITVGGSGRGFYM
                     AGGAQGETKEMYWVDLGARINVLNKKGTITVRVSDIFNTQKMKYSSWDTNFYSYNESW
                     RDSRVVFVGFSYRINNYKMRPEKKVDSDDSFDMVE"
     gene            complement(11675..13003)
                     /locus_tag="PK049_01490"
     CDS             complement(11675..13003)
                     /locus_tag="PK049_01490"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOK60258.1"
                     /translation="MKALTNTARNFFLPLLLIFTINCYSQGSEDRMQWFSDAKFGIFI
                     HWGIYSLGNTSESWAFYNKQVSHADYMAQINLFSAERYDPAQWARLVMQSGARYVVIT
                     AKHHDGVALWDTKLSNLSMATQSPAARDVLSPFIKALREQGLKVGIYFSLIDWTHPDY
                     PGFTRDSNRYNVSDDTLRWQQYLNFCHGQIEEIMQLYAPDLIWFDGDWEHSAKEWQAE
                     KIREIIHRHNPNTIINGRLQGYGDYDTPEQNFPVTRPNLKYWELCMTMNSSWGYRVND
                     TIYKTPFEIVSILTDAISMGGNLLLDITPKADGSIPEEQVKTLSTIGNWIGKHSDAIY
                     GTLPGLPMGHFYGATTLSKDSTSLFLFLPAKTYGQIMLKGINSPIKKATVLGTNHELL
                     HKVVGKISWSPVPGLIFIDVPKELTDALMTVVRIDFDEPIKLYRGKGGLN"
     gene            13128..14540
                     /gene="nhaD"
                     /locus_tag="PK049_01495"
     CDS             13128..14540
                     /gene="nhaD"
                     /locus_tag="PK049_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter NhaD"
                     /protein_id="HOK60259.1"
                     /translation="MFLLMVVLFVLGYAAIALEHNIKVNKTASALFLGVALWVLLMLD
                     AGGILGTDIGGRFGEFINSHPELASLPKWEQYVNYIADNQILGFLGEISEILFFLMGA
                     MTIVEIVDQHGGFKIITNRIKTRNKVKLLWIISFVTFFLSAVLDNLTTSIVMVALLRK
                     LIDGKHDRWLYAGMVILAANAGGAWSPIGDVTTIMLWIKGNVTTAGIIPKLFFASLVS
                     LIVPLVALSFTLKGDFAQSLDSSSEKVHSVTPATRRERHLIFILGVGALLFTPVFKVL
                     THLPPFMGMLLGLSVLWIFTEVMYHRKHNLDEREKRTVARVIKNVDVPTILFFLGILM
                     AVDALQTAGHLTLLSQWLDRSFTSPYPPNILIGLLSSVVDNVPLVAAAMGMHPAVSPD
                     LVNTASAISSAYVADGDFWNLLAYCAGTGGSILIIGSAAGVAVMGLEKIDFMWYLKHI
                     SLLALLGYFAGILTYFLMFG"
     gene            14650..15774
                     /locus_tag="PK049_01500"
     CDS             14650..15774
                     /locus_tag="PK049_01500"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOK60260.1"
                     /translation="MSIQIVKVENKTDLRKFIHLPAIIHKGHKNWVPPIYSDEWDFYN
                     PRKNRAMSYSDTVLYLALNNGRPVGRIMGIINRRYNQIHNEKTARFFNLETFQDYDVA
                     QALLGEVEKWAKEKGMDRLIGPMGFSDKDPQGLLVEGFHEPIVLASNCNYPYLVEFVT
                     RYGFEKDIDLVVYKVPVPHELPDFYLRILQRVKSRANGFRMLSFSKRSDIKPFIKPVL
                     SLVNETFVDIYGFNPMDSDEMEELAAKYLSFIDPRFLKVIVNTHNQPLAFILGIPDIC
                     PGIQRSKGYLFPLGFIHILRAMRTSKTLSLLLGAIHPQYRNLGFDTWMGAEMLHEAQK
                     AGMTIIDSHLELETNRKMRAEMEKMGGVVYKRYRIFQKKL"
     gene            complement(15812..18394)
                     /locus_tag="PK049_01505"
     CDS             complement(15812..18394)
                     /locus_tag="PK049_01505"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HOK60261.1"
                     /translation="MKRAITLLLSFTIGILFSHAQVITSPEEHFGFTPGADRMLFKYD
                     QVVEYLRKVETQSPMVKLTPIGQTAMGKNLYAVFVSSKENIANLNRLKEINKQLAINY
                     SLNNAEREQMVAQGKVFVMATLSMHSTEVGPTQALPTIIYRLISGNTFSNALNNVVYM
                     AVVCHNPDGMDMVVDYYNQFKGSKYEGGPMPFLYQKYVGHDNNRDFITLTQPETKAIS
                     RLTSQEWFPQVMVEKHQMGLTGPRYFVPPYHDPIAQNVDAELYAWSNLFGQNMLNDLT
                     SKGLQGVSQQNLFDNYWPGSTETCLWKNVISMLTEAASARVATPVYIEPTELTGTSKG
                     LAEYKKGVNMLLPWPGGWWHLSDIVALEVESTISMLQTASKYKERILMFRNDICKKQV
                     SLGKTIAPYHYILPANQQDPSELVSLVNLLKEHGVNVYRLKQNITIQNQNFLSGDIVV
                     PLAQPFRMFIKEVMEPQEFPERRLEEGGKVLEPYDITSWSLPLHKGLTCFTINNNAID
                     NSIIEPIQSSFTLNQKTLPEIGFAILPSTVNQSYKLAVKCLKSGVQVERTNEHTVFDG
                     IELPAGSFCIPLNEKTRQILNDANFIIMTSDVKPNNTSISKIRRIGLLESQVQSMDAG
                     WTKYILDTYEIPYTVINPSQVAEGNLLSKFDLVIIPNTNATLLKDGKYKRNGQVIPSV
                     YPEIYTKGMGQKGIEALTEFIQNGGTVLAWEQATELFEGTLSLKQGNVSEEFMLPFKN
                     NAKEVQKQGFSCPGSLVRLKIKPVYPFTFGLADELGIFMRDSYLFETQVPSLDMDRRV
                     IGYFEGKSKLLSGYLKGEEIMDGMTAAIWLRKGKGQVILLGFSPIFRASVPSNYKILF
                     NTIL"
     gene            complement(18506..18838)
                     /locus_tag="PK049_01510"
     CDS             complement(18506..18838)
                     /locus_tag="PK049_01510"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOK60262.1"
                     /translation="MIDVEKINDVMVISFSENSKLNVTVTQKIKTEVIKVITPNAKVA
                     LNLSGVSYIDSTGFGMLLSILRHCKNSNATLKLCNISPEVMELIKLLQLQTVFDIRES
                     VDDCIKSF"
     gene            complement(18864..19226)
                     /locus_tag="PK049_01515"
     CDS             complement(18864..19226)
                     /locus_tag="PK049_01515"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOK60263.1"
                     /translation="MATDQATHHVNLDFLKEISEGSTDLMRDLINLFITQVPTFSEQM
                     EIYLKNQDYCTLAKLAHKIKNSVAMMGIDELTSDMKKLENICTSKPNDDTVKVYVERF
                     KKISGEASVELQHILESL"
     gene            19387..20496
                     /gene="gcvT"
                     /locus_tag="PK049_01520"
     CDS             19387..20496
                     /gene="gcvT"
                     /locus_tag="PK049_01520"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOK60264.1"
                     /translation="MKNTPFTKYHIELGARMAPFAGYNMPIEYSGINDEHITVREKVG
                     VFDVSHMGEIWVKGPKALDFLQTVTSNDVSALFDGKVQYSCFPNDKGGIVDDLLVYRV
                     NAETYLLVVNAANIEKDWNWLCEQGKKFGLTPGKELYNASDEFAQLAIQGPLALKAMQ
                     KLTTENVVDMEYHTFKKIPFAGIKEVIFSTTGYTGAGGCEIYCANEDADKLWKAVFEA
                     GAEYGIKPIGLGARDTLRLEAGFCLYGNDINDSTSAIEAGLGWITKFVPGKNFISRDV
                     WEKHKNEGTDRRLKGFVMIDRGIPRQHYLIYDAQGNEIGEVTSGTMSPMSKQGVGMGY
                     IKKGHWNDGNEIYIGIRDKKLKAKIVKPPFHLAKV"
     gene            20543..21256
                     /locus_tag="PK049_01525"
     CDS             20543..21256
                     /locus_tag="PK049_01525"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HOK60265.1"
                     /translation="MPRIEVAFSPTIFQHYDKEGKIVVVVDILRATSVICTMFHNGVK
                     EIIPVRSIEEAHEYKRNGYLVVAERNGQKLDFADFGNSPFYFTPETVNGKTIVYSTTN
                     GTNAITLGRDAQEVVIASFLNISAVESYLNARSSDILIMCAGWKGKFCLEDAIFAGAL
                     AQRLLTTGRFETICDSTNAAIDLWNVAKVDLTSYVEKVAQRHRLKKLGLDDVIGYCFT
                     SDVTDTIPVLKEDKLVPLI"
     gene            21388..22458
                     /gene="serC"
                     /locus_tag="PK049_01530"
     CDS             21388..22458
                     /gene="serC"
                     /locus_tag="PK049_01530"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HOK60266.1"
                     /translation="MKKHNFNAGPCILPEVALENTAKAVLDLDGIGMSILTISHRSKE
                     FEAIMEETKALWRELLHIPENYHILFLGGGASLQFCMVPYNLLQSKAAYLDTGVWANK
                     ALKEAKLFGEVVTVASSSDKNYTYIPKGFTIPTDVDYFHITTNNTIYGTEIKHDMDSP
                     VTLVADMSSDIMSRPVDVKKYGLIYGGAQKNVGPAGVTFVIIREDILGKVERKIPTML
                     DYRTHIKDGSMFNTPPCGAIYTMRETLKWLKGIGGVEKINELNKKKAQLLYDAIDNSK
                     MFVGTAEKDSRSIMNICFVMKPEYKDKEDAFMDFAKKAGMVGIKGHRSVGGFRASTYN
                     ALPIESVQALVDCMKEFEKQNA"
     gene            22877..23800
                     /locus_tag="PK049_01535"
     CDS             22877..23800
                     /locus_tag="PK049_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HOK60267.1"
                     /translation="MTKVLVATEKPFAKVAVEGIRKIVEDAGFELVLLEKYTDPNDLV
                     KAVADVDALIVRSDKVTKEVVDAGKNLKIVVRAGAGYDNLDLPACTAKGVVAMNTPGQ
                     NSNAVAELAIGMMIYMARNLFQPGTGTEIAGKKLGIHAYGNVGKLVAKYGKGFGMEVY
                     AFDPFVDKAVMEKDGIKVVDSIEMLYSSCDYVSLHIPANDKTKKSIGYDLLSKMPKGA
                     TLVNTARKEVIDEDGLKKVLAERTDLKYATDIMADCHAELVEKFPGRVFATPKKMGAE
                     TAEANINAGLAAARQIVAFIKNGDKTFQVNK"
     gene            23850..24197
                     /locus_tag="PK049_01540"
     CDS             23850..24197
                     /locus_tag="PK049_01540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOK60268.1"
                     /translation="MDHKELDAWKVAVDLSVEIYSLTQSFPKEEIFGLVQQIRRSAIS
                     IPSNIAEGCARGSSKETIHFLYVSLGSLAELETQIIIASKLGFVNGIDELLKKLNKLK
                     QVILGLIRYLKTV"
     gene            24229..25470
                     /locus_tag="PK049_01545"
     CDS             24229..25470
                     /locus_tag="PK049_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HOK60269.1"
                     /translation="MVKIKAFKGIRPPREIAKEVAARPCGVLNSQEAKAEAGEKSLLH
                     ITKPEIDFDPIIDEHTQQAYDKAVENFRKWKERGWLVQDPKDMYYVYAQTMDGRTQYG
                     LVVACHFEDYMEGRIKKHELTRPDKEEDSMIHIRIQNANIEPVFFAYPAHKEMDEIVT
                     NVVTSKKPEYDFVADDGFGHHFWVIDDEATIKRITEIFASIPALYVADGHHRTAAAAR
                     VGLEKKNANPNHRGDEEYNYFLAVIFPDNQLKIIDYNRVVKDLNGLTPEQFLQKLNEG
                     FVVEEKGSEIYKPQQLHNFSMYLEGKWYSLTAKPGTYNDNDPIGVLDVTILSNLVLDP
                     ILDIKDLRTSKRIDYVGGIRGLGELKKRVDSGEMKVAFALYPVSMKQLIDIADTGNIM
                     PPKTTWFEPKLRSGLVIHALE"
     gene            25613..26284
                     /locus_tag="PK049_01550"
     CDS             25613..26284
                     /locus_tag="PK049_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOK60270.1"
                     /translation="MSIPEQLEKIKNELPQHVKLIAVSKTHPVSVIMEAYNAGHKFFG
                     ENRVQELISKYDEMPKDIEWHLIGHLQTNKVKYIAPFVSLIHSVDSLKLLSVIDKEAQ
                     KCNRVIDCLLQVYIASEETKFGLSADELHELLQNQEFEQLQNVRVCGLMGMATFTDNM
                     EQVRMEFRFLKKLFNEIKQTYFQNQPWFNELSMGMSSDYRIAIEEGSTMVRIGSNIFG
                     HRSYT"
     gene            26329..27306
                     /locus_tag="PK049_01555"
     CDS             26329..27306
                     /locus_tag="PK049_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007485907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HOK60271.1"
                     /translation="MAKLETSYLGLSLRSPLIVSSSGLNSSVDRIKKYQEFGAGAIVL
                     KSLFEEQILHEVNHLEKYSDYTEATDYLKHYVSANNVQEYINLIKNAKQSVDIPVIAS
                     INCLSDNQWLSFASRIQEAGADALEVNIYYLPLDPKRKAEEYEQVYNSIAEKLQKVIT
                     IPVAYKLGSHFTNPLNVVEHLRYRGAKGVVLFNRFFEPDIDIHAMKVVPADIFSNPSD
                     LRYTLRWVAIASHLIPEIDISASTGVHDGEALVKMLLAGAKSVQVCSAIYRNGPKHIE
                     AMLNTLTEWMNQKGFKTISDFRGKLNVGSIADPTQYERSQFMKYFSNYE"
     gene            complement(27339..27845)
                     /locus_tag="PK049_01560"
     CDS             complement(27339..27845)
                     /locus_tag="PK049_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60272.1"
                     /translation="MQPNKAEQFQKTIEASSDEAIIGLLTELRETGEDYMLPTILSLL
                     FSTRSEKLKNEVVNFLVDLKNKSSIDTIVEAIRQNKRSKDIHLLVSVCWQSRLDFTPY
                     VDTFIDIMEHGNYQACLEAFTVIENMTENLSGEQLNDLKAKIKSVKGDNAETKPLIEE
                     LVKLLDEL"
     gene            28002..28919
                     /locus_tag="PK049_01565"
     CDS             28002..28919
                     /locus_tag="PK049_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60273.1"
                     /translation="MHKGSKRILLLSAMAAMAVLTISCKCNRESEQMLQVSEEQGAAD
                     SALVSSIESVKQIFYSLPSPLETAMILKRSGATYNEELLNPIENASKYNTTKSMALNL
                     GIYSTDLSYASLFDQTQATIKYMTVSKKMAEGLGILNAIDNSVIEKLEENINNRDVIL
                     ETISETFLNTNSILEEDDRVAVGSIILVGGWIEGLYIATSLVEDINKVDNEMVDRIID
                     QKLSLQTVLKLLEQSNSNADVRELYSDMLELEKVYNDVKITVSDVKVIQDGKSKVATL
                     KSKNVTTVSPEAFKKLKTKVIELRNKYTA"
     gene            28930..29373
                     /locus_tag="PK049_01570"
     CDS             28930..29373
                     /locus_tag="PK049_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60274.1"
                     /translation="MRSSIYILVSAVVLFLALPSSSNAQCKNFAKKICRLELSPYVHD
                     GNYNAAILTEGEDAELFKTFYAGQQYRISVCGSETLNKIEFMVMDVDRKVLYTNAKNN
                     FATSWDFKVENTQQLIILVKVPVTSGKNEELKSGCVAIMFGLKTD"
     gene            complement(29432..32227)
                     /gene="uvrA"
                     /locus_tag="PK049_01575"
     CDS             complement(29432..32227)
                     /gene="uvrA"
                     /locus_tag="PK049_01575"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOK60275.1"
                     /translation="MQKHEKVISVKGARVHNLKNINVDIPRNKLVVITGVSGSGKSSL
                     AFDTIYAEGQRRYVESLSAYARQFLGRMSKPEVDLISGIPPAIAIEQKVNTRNPRSTV
                     GTSTEIYDYLKLLFARIGKTYSPISGHEVKRHSVTDVVNFISAHQQGTRMTLLAPIAV
                     NGNIKLKLEELNREGFTRIDINGKVIRIDDAQNRELVDLAAANTRLIIDRFAVSVDED
                     FISRIADSVQTAYAEGNGFCILSIETDEGIREEQFSNLYELDGIKFEQPTEHLFNFNN
                     PLGACPKCEGYGKVIGIDEDLVVPNKSLSIYEDAIACWRGESMGWYKDRVIEYASAYN
                     LPIHTPYHKLTEEQKALLWNGTRHFKGIYDFFAELEEKRYKIQYRVLLSRYMGKTTCP
                     VCKGGRLRPEASYVKVAGYTIQELVNIQIEELYEFFMNIELDEHDVTVAKRLLTEIRN
                     RLGYMVNVGLGYLTLNRLSATLSGGESQRINLATSLGSSLVGSLYILDEPSIGLHPRD
                     THLLIDVLKNLRDLGNTVLVVEHDEDIMRNADYIIDIGPHAGLLGGEVVFAGEPSLIN
                     SASTSLTAKYLNGTEKIEVPFKRRKWNSYIEVKGARENNLKNIDVKFPLNTLTVVTGV
                     SGSGKSSLVKGILYPALLKEITGSGERAGQHDCIAGDIRLISGVEMVDQNPIGKSSRS
                     NPVTYLKAWDEIRKLLSEQPYSKSNGYGPSHFSFNIDGGRCEECQGEGVIHVEMQFMA
                     DLTLTCESCHGKRFKDDILEVRYRNANVYDLLEMTVNQAIEFFEKGKGSLEKKIVEKL
                     KPLQDVGLGYIKLGQSSSTLSGGESQRVKLASFLLKENTDKPILFIFDEPTTGLHFND
                     IKKLLSAFDALIAKGHSIVLVEHNLEVIKCADWVIDLGPEGGDNGGHLIFSGTPEQLA
                     LMDNSYTGKFLSVKLNN"
     gene            complement(32483..33124)
                     /locus_tag="PK049_01580"
     CDS             complement(32483..33124)
                     /locus_tag="PK049_01580"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HOK60276.1"
                     /translation="MSSKTILLSTAYLPPVHYMSALLSADKIIIEAHETYLKQTYRNR
                     CVILSANGPLPLVIPVIKPKGNHTGITEVIIDYSTGWNRNHWRAIESAYRTSAYFEFI
                     ADVLKPFYSNPFNTLWEFNNELLKAILDFLELPVNINFTSTFHKVEPEGVLDLRYAIS
                     PKAKMPETSLHATLPYFQVFEHRFGFTPNLSVIDLLFNEGLMARDILRIKDKG"
     gene            complement(33204..34541)
                     /gene="lepB"
                     /locus_tag="PK049_01585"
     CDS             complement(33204..34541)
                     /gene="lepB"
                     /locus_tag="PK049_01585"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOK60277.1"
                     /translation="MFQKVKDFLKNKYFRFTFAAIIFILWVVWIGNYWLLLGLPIIFD
                     FYITQKVNWTFWKKRGQKKTVLIEWVDAIIFAVVAATLIRMFFIEAYTIPTSSMEKSL
                     LVGDYLFVSKVAYGPKLPNTPISFPFVHHTLPFTKYTKSFIEWPRWPYKRIAGFGQIK
                     RNDVVVFNFPEGDTVCLQDQNSSYYTLARIYGRDYIRQNYDIIYRPVDKRENYIKRCV
                     GIPGDTIQVVHGSLFVNGKEQAKIGKRQYNYQIRTNGSTINPIRFEEMGIARSDINYN
                     AQESVYILPLTEEMLERFKTFSNVTEITRNENTDSTVMWKLIFPHDPRYKWNEDNFGP
                     LWIPKKGATVTLTTDNLPLYQRIIDVYENNDLRVENGTIYINGEPASSYTFKMDYYFM
                     MGDNRHNSADSRFWGFVPEDHVVGKASFIWLSLDKDKRFPANIRFKRMFTTIK"
     gene            complement(34553..35275)
                     /gene="dapB"
                     /locus_tag="PK049_01590"
     CDS             complement(34553..35275)
                     /gene="dapB"
                     /locus_tag="PK049_01590"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOK60278.1"
                     /translation="MLRIALIGYGKMGHEVEAIALERGHTIVLRIDKDNINDLNTENL
                     KKSDVAIEFTSPSTAMQNIQACFDAGIPVVSGTTGWNNQLEIMVNKARNGVGTLFYAS
                     NYSIGVNIFFKLNNYIASILDRIGMYQPSITEIHHTQKLDAPSGTAITIANTLCNQMK
                     AYEGWSLIPDSAERKIPIEAIREGQVPGTHIVTFTSEQDIITLKHEAKSRRGFALGAV
                     LAAEFLSSRKGFYSMDDLLKLD"
     gene            complement(35406..35750)
                     /gene="rplT"
                     /locus_tag="PK049_01595"
     CDS             complement(35406..35750)
                     /gene="rplT"
                     /locus_tag="PK049_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOK60279.1"
                     /translation="MPRSVNAVASRKRRKKILKQTKGNFLARRNVWTVAKNTLEKGLT
                     YAYRDRKKKKIEFRALWIQRINAAVREYGMTYSEFMGKINQKGIAINRKVLADLAMNH
                     PEAFKAIVDSVK"
     gene            complement(35862..36059)
                     /gene="rpmI"
                     /locus_tag="PK049_01600"
     CDS             complement(35862..36059)
                     /gene="rpmI"
                     /locus_tag="PK049_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOK60280.1"
                     /translation="MPKTKTHSGSKKRFALTGTGKIKRKHAYKSHILTKKETKQKRNL
                     THVALVDKADEGHVKAMLGLK"
     gene            complement(36094..36630)
                     /gene="infC"
                     /locus_tag="PK049_01605"
     CDS             complement(36094..36630)
                     /gene="infC"
                     /locus_tag="PK049_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOK60281.1"
                     /translation="MNKKSDIDTYPINEEIRAKTVRLVGENIENPGIYPISQARQMAE
                     EMELDLVMISDKADPPVCKILDYQKFLYQLKKKQKEIKANAQKIVVKEIRFGPNTDDH
                     DYNFKLKHAINFLQEGAKVKAYVFFKGRSILFKEQGEILLLRFAQDLEEYGKVEQLPQ
                     LEGKRMIMIVSPKGTKKK"
     gene            complement(36675..38615)
                     /gene="thrS"
                     /locus_tag="PK049_01610"
     CDS             complement(36675..38615)
                     /gene="thrS"
                     /locus_tag="PK049_01610"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOK60282.1"
                     /translation="MIKITFPDGNVREYPKGVTGLEIAKSISERLAADVLSVSVNGEV
                     WDLTRGIETDATVKLHKWDDPEGKHAFWHSSAHLMAEAVEALYPGVKLGIGPSIENGF
                     YYDIDFNGHTISEEDLPKIEQKMMEFAQQKQPIVRREVSKAEALKTYQEKGDPYKVEL
                     ISDLQDGTISFYTQGEFTDLCRGPHLPNTGYIKAIKLLSIAGAYWRGDEKNKMLTRIY
                     GITFPQKKQLDEYLTLLEEAKKRDHRKIGKDLELFMFSPSVGPGLPLWLPKGAQLRER
                     LEMFLKRVQKKYGYEQVITPHIGQKELYETSGHWAKYGKDSFRPITTPQEGEEFLLKP
                     MNCPHHCEIYKSKPRSYKDLPLRLAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFCR
                     PDQLKEEFVKVIDIVLYIFKTLDFKDYTAQISLRDPNNKEKYIGSDENWEKAEKAIVE
                     AAAERGLKTVTELGEAAFYGPKLDFMVRDAIGRKWQLGTIQVDYNLPERFQLEYIGAD
                     DKRYRPIMIHRAPFGSMERFVAVLIEHTGGKFPLWLTPDQVVVLPISEKFNDYAKSVS
                     KFLNNYDIRSLVDDRNEKIGKKIRDNELRRIPYLLIVGEKEQEANLVAVRVQGEGDKG
                     QMKLEEFVNHIKAEIDNQLKEI"
     gene            complement(39128..39310)
                     /locus_tag="PK049_01615"
     CDS             complement(39128..39310)
                     /locus_tag="PK049_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60283.1"
                     /translation="MGTPRVELPRDTIRHAYGVTHVVSLTKPTATFHPRPIAGSGFYA
                     SACFFRSIPSQGKGMR"
     gene            complement(39662..40312)
                     /gene="plsY"
                     /locus_tag="PK049_01620"
     CDS             complement(39662..40312)
                     /gene="plsY"
                     /locus_tag="PK049_01620"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HOK60284.1"
                     /translation="MLKIAVIFAAYLLGSIPTSVWVGKIFYGIDVREHGSGNAGATNT
                     IRVLGLIPGISVFIVDVLKGYLAVRLLYLTDFYIPKTGMFINFQLFLGIAALLGHIFP
                     IFAQFRGGKGVAVLSGVVFALHPYATLIVFGIWIVSVLITRYVSLSSMIAGFSFPLVL
                     IFVYRTTNPSLITFAFILAILMLFTHQKNIERLVKGEESKFSLKKKGKTNQVNPKQ"
     gene            complement(40496..43381)
                     /locus_tag="PK049_01625"
     CDS             complement(40496..43381)
                     /locus_tag="PK049_01625"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOK60285.1"
                     /translation="MDSTDTQYIFAIALHRHRELGLVAGAYLISPISDDTYKIEKNVI
                     VDSAVIHSDRITTDQSEIIRSIQEISEKSLLKLFGRDAKTFNELLQKVQSDENLNSYI
                     RNYIDRRICKAVEKAASANIPIYIRERNANNIYKEKQLTYHTDVASPIFRFELDELGM
                     KYSLKIQHQTSILTLLPGVFELLTVEPCLIRVANGIYKVEGIDGRKFVPYMTKQFVTI
                     PQQSVQKYMETFVLNAVRSNQVEAVGFEINEQKNRPKPILTLDERLSGGACLTLSFQY
                     GNKVISANTPAVNEVKLSFRNNSYTFTRLTRNPEKEEKIKQQLIKTWRLTKVGENEYF
                     PGKPENGEFDHYQVIEWLNTNYKLLQDEGFEIHQKFRQTNYYLSGFDLNLRLTESIDW
                     FDLYGMVRIGEFEIPFVYFRKHIVKNIREFTLPNGQVFILPDEWFAKYRTILMLGKEE
                     GESIKLSKASAAIIAGSSLETQSISSFAEKLKNINAKGVTLPHSLQAKLREYQTLGFA
                     WLKTLVENGLNGCLADDMGLGKTLQTITLLLSELEANDFKTGQEHCSLVVAPTSIIYN
                     WQKEFEKFAPQVKVGIYHGTQRDKSHKIFQQNHVVITSYGTIRNDIDLFKDFAFRYVV
                     LDESQTIKNPASKIYRCVLLLKTKKSLCLSGTPIENNLFDLWSQMNFLNRGMLGGLKV
                     FREEFAIPIERRNDAEKQKQLKKLIEPLIMRRTKEQVAKELPPLTEQIIYCEMTDEQG
                     KAYEAEKSKARRLILDSLEQSKPESSTINVLSALTRLRQLANHPAMLDEYSNLSSGKF
                     EEVTQMIESVIAENHKILIFSSFVKHLVQVETYLQNNGIGYEMLTGSTTDRSDVVEEF
                     QNNPEKKIFLISLKAGGVGLNLTAADYIFILDPWWNPAAEMQAASRAHRIGQTKSVFV
                     YRFITRNTVEEKIVKLQAHKENLAHEFVNSNNPMILLNKEQLISLVE"
     gene            43564..45414
                     /locus_tag="PK049_01630"
     CDS             43564..45414
                     /locus_tag="PK049_01630"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOK60286.1"
                     /translation="MIKRILALLTLVSLSYILFSQEVEVKISKDKVRVDGKTYFVHIV
                     QQGETLYSISKAYGVSQSDIAINNPDIYAGLKVGQALKIPAKVKETQTDEDYIYHIVK
                     RKETLFGISRMYNVSMDDILRLNPEAREGLKLSQTLRIPKKQISDIEQQPAVDSVEFI
                     LHEVQPREGLFAISRKYGVSPKEIEYYNYDLIKDGLKLGTTLRIPKPKSVPVPEAVEA
                     KVDTDGQEKASPCSNSYTYDGHPFNVSLLLPFTQSGSQTQAIVDDVDPKDVQDPTGQL
                     TQITQVSLEFYEGFLLALDTLKKLGISVNLNVRDTKRSPQEVAKILNSADFARSELVV
                     GPYMFDEVKPVAQFAAKNNINFVSPIYSIKEGLADKQNIISVSQTLQNQLEIFVKNLK
                     VDPSKNYLVIYDSTIWYSPSLKHFDSLLTLSFTNAAIPISRYNHKTAAYKSVDVQERL
                     LKMLKRDAVNVVIVPSDDEPFVAEILGSLYAVKSYYNLNTEVYGPSRWRKLKNLPPDY
                     LFALNTHVFSPFFVDYSQSDVKTFVEKYRVQFRSEPTQFSFLGYDVAFYFINALKEFG
                     PGFTPCLPNFRLRTLQTNFKLNTSKEGYTLNSNQFIVRYAPDFTITCSCY"
     gene            45438..46340
                     /locus_tag="PK049_01635"
     CDS             45438..46340
                     /locus_tag="PK049_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOK60287.1"
                     /translation="MNYRFNSGNTRLVNFEGWLSVLINTLLFVLKYWAGVVTGSLALI
                     ADAWHTLSDSISSVVLLLGIKFSQKPADKDHPFGHGRSELVVSLLIGALLAFVAFYFA
                     MEGIAKIRDRVSTSYGTLAIVATVVSIVVKEGLAQVAFIIGKRTGSISVTADGWHHRS
                     DALSSVIVLVGIFVGKYFWWIDGVLSIIVALLILYSGIKVITDSAVVILGEVPSDDLV
                     QKVMEIVSRQGYDRMEPHHFHIHNYVMHQELTFHVRLPNQMTIEQAHRIISLIEDAIH
                     LELGIDATIHIEPQTTDKHCKHID"
     gene            46490..48106
                     /gene="pckA"
                     /locus_tag="PK049_01640"
     CDS             46490..48106
                     /gene="pckA"
                     /locus_tag="PK049_01640"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018361136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HOK60288.1"
                     /translation="MEMIDLSQYGINSLRELYYNPSYDLLYEHETDPNLEGYERGFVT
                     KLGAVNVDTGVFTGRSPKDKYVVREPNTENDVWWAESGKKGSDNKPISQEVWEKLKEV
                     SVKQLNGKKLYVVDAYAGANEDTRLSVRFIMEVAWQAHFVKNMFIRPSDEELKNFTPD
                     FVVLVASKTTNPYWKEQNLNSEVYVAFHLTERMALIGGTWYGGEMKKGIFSVMNFYLP
                     LKGIASMHCSANVGKAGDVAIFFGLSGTGKTTLSADPNRALIGDDEHGWDDSGVFNFE
                     GGCYAKCIDLDPQKEPDIFAAIRRDALLENLDVDAEGNIDFHSKRKTENTRVSYPIYH
                     IKNIVKPISRAGHAKNVIFLTADAFGVLPPVSKLTPNQTQYHFLSGFTAKLAGTERGV
                     LEPQPTFSSCFGQAFLMLHPTVYARELVKKMQQHNSKAYLVNTGWIGGAYGVGRRIDL
                     PSTRAIINAILDGSLDNAEFDVLPIFNLQIPKALPGVDSKLLNPRNHWSNPEDWDAAA
                     RNLAQKFIENFKTFTDNEWGQRLVAAGPML"
     gene            complement(48190..49137)
                     /locus_tag="PK049_01645"
     CDS             complement(48190..49137)
                     /locus_tag="PK049_01645"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOK60289.1"
                     /translation="MKAITRTDFKFPGQVSVYHGKVRDVYNINNQFLVMVVTDRISAF
                     DVILPKGIPYKGQVLNQIANKFLDATADIVPNWKIASPDPMVTVGRFCEPFKVEMVVR
                     GYLTGHAWREYRSGKRSLCGVPLPEGMKEHQKFPKPIITPTTKAEEGHDEDISREEII
                     AKGLVSEADYNLLEKYTLALFERGTQIAEKMGLILVDTKYEFGKKDGKIYLIDEIHTP
                     DSSRYFYLDGYKERFEKGEEQRQLSKEFVREWLMANGFQGKEGQTVPEMTPEFIEQVS
                     NRYIELYEKITGDRFQKAHSDEDILNRIEKNVKAFLQNM"
     gene            complement(49149..50150)
                     /locus_tag="PK049_01650"
     CDS             complement(49149..50150)
                     /locus_tag="PK049_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018695335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOK60290.1"
                     /translation="MAAGERTIIIEGIDPLVLYGVNNTRFDLITKHYPKLKIIGRGNT
                     IKAVGDEQLIEDLDQKVHAMIDFHIKYGYLSETNIKDILQNNPESIEKAEEDITDVLV
                     YGNHGKIVKARTVNQRKLVEMYESNDLIFAIGPAGSGKTYTAIALAVRALRNKEVKRI
                     ILTRPAVEAGERLGFLPGDMKEKLDPYLQPLYDALHDMIPPKKLQEFMEDGTVQIAPL
                     AYMRGRTLDNAFVILDEAQNTTLSQLKMFLTRMGSNAKFIVTGDITQIDLPNKDDSGL
                     VKAIKLLEGIKDIAIIYFDTRDIIRHKLVKYIVNAFEEAKGKTDIQTDAKSDDNQTK"
     gene            50324..51127
                     /locus_tag="PK049_01655"
     CDS             50324..51127
                     /locus_tag="PK049_01655"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HOK60291.1"
                     /translation="MAATNTRFVTLTTDWGIQDYYLGMLCGKLYSISPNLKVVELSHK
                     VPTYNYMHAAFLIKHSFSHFPKGSIHICMVNSDTSDNMRMLFFEYQGHYFITPDNGTI
                     SLIADVLPEVVRAIPVDDTRSFGSIDSVTLALEALLSENGQTDFAIEASGIKRFTPIL
                     PVPENDGITGSVIYIDSYFNAITNITRDDFEAIRKSRKYSIYVHRFSHGISKLSNSYK
                     DVEKGDLLALFNSLGLLEIAVREGFAAQMYNLSVGSNIMVRFYDDKILR"
     gene            51127..51921
                     /gene="mazG"
                     /locus_tag="PK049_01660"
     CDS             51127..51921
                     /gene="mazG"
                     /locus_tag="PK049_01660"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOK60292.1"
                     /translation="MNDSVRKQRASEAFQRLIGIMDDLRAKCPWDREQTFESIRPLTI
                     EETYELSEGILEKDYNLIRKELGDLLLHIVFYSKIAEEDNRFDIVDVINSLCDKLIYR
                     HPHIYGNVKVNDASEVVENWEQLKIKEKDGNKTVLSGVPTALPALIKANRIQEKVRAV
                     GFDWDERSQVWDKVQEELNELRHEVEVTKSTDDIEKEFGDLLFSVVNAARLYGVDPET
                     ALERTNRKFMKRFTYLEQQTIQMGRSLKDMTLDEMNEIWEEAKRFD"
     gene            51934..53016
                     /locus_tag="PK049_01665"
     CDS             51934..53016
                     /locus_tag="PK049_01665"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOK60293.1"
                     /translation="MFLYLVRKLFYTFLVIFGVASLVFILFNLIPGDPARMVLGQRTD
                     SLSVKAARTDLGLDLPVWKQYLKYLNDISPLSVHNPSNSKSSIYLDTLIYGKPIVLGK
                     MASNRVLVAKQPYLRRSYQSRQPVSTVVMSTLPNTFYLALVAIVFATIVGIAMGITAA
                     IYKDSVWDRLLLVNSALGMALPSFFAAILIGWLFAIVLGKYTGLNLTGNLWEVDDLGE
                     GIHLKWQNIILPALTLGLRPLGIITQLMRSSLLETLSQDYIRTAKAKGLPNRKVIFKH
                     ALRNSLNPVVTSISGWFASMMAGVVFVEYIFSWKGLGYVMVNALNSYDVPLVIGCVLT
                     VAVIFSIVNLLVDILYGILDPRVRIG"
     gene            53098..53853
                     /gene="tpiA"
                     /locus_tag="PK049_01670"
     CDS             53098..53853
                     /gene="tpiA"
                     /locus_tag="PK049_01670"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012457684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOK60294.1"
                     /translation="MRRKIVAGNWKMNTLPSEGLALAKDVIAKSAEVSSGVELVVIPP
                     FTHLNEVTKLASGTRVAVGAQNCARWQSGAYTGEVSAAMLGAMGVEYVIIGHSERREY
                     FGETNSQLYDKIQQALSSNLMPIFCCGEKLDEREANQHFQVVKQQVEESLFALDEQSI
                     KQVVIAYEPVWAIGTGRTATAEQAQEMHAYIRKLLAGKYGSQIADSISILYGGSCKPS
                     NAAEIFAKPDVDGGLIGGASLVAADFIAIAKSF"
     gene            53855..54691
                     /gene="prmA"
                     /locus_tag="PK049_01675"
     CDS             53855..54691
                     /gene="prmA"
                     /locus_tag="PK049_01675"
                     /inference="COORDINATES: protein motif:HMM:NF001785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOK60295.1"
                     /translation="MGYTELKIRIKPYTAETAEILIAQLAELGFESFMENEPELLAYI
                     NTATLKKLPADFIGLIELPEGVRIDYELNSIADKNWNEVWESNFEPIIVDNRCLVKAS
                     FHTDTPKTDYEIVIDPKMSFGTGHHQTTHLMIEALLNHQVEGLTVLDMGCGTGVLAIL
                     AEMRGAQNIVAIDNDEWAYRNTLENIDNNSCKRITAYLGDAELLKGRSFDLILANINL
                     NILLADMAAYVNSLKSGGVIFMSGILRDDVETLRNAAERLGLSFSEVNYRDNWAMVAF
                     RK"
     gene            54716..59302
                     /locus_tag="PK049_01680"
     CDS             54716..59302
                     /locus_tag="PK049_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60296.1"
                     /translation="MAKPYVYRRILIIALLLTAFSLLYAVFISQDKKFSGQVPLFPAE
                     LNSLVSDKVKEQEEFFLKGFPAFWLSDTIEISKKEAIIGTANQILEVLPDDKSYILSY
                     VKLIADFYRNQYAKNQFETWFSYLNMALADGFIKSDNLRTLIRFSGSLIDSSFISIST
                     SHTWKVLPANSYTFTLKNNDLLVSFQNVNLICRNNRTDSIFIRGTSGNLDIIKQSWEG
                     KNGKVTWIRSKFDESKIFVALNRYRIELRQSEYTADSVLLNYPEYFKKPILGRIVDKV
                     TPIYQSGIVDYPEFNPYQKWFEVKNIFKDVDYSGNFRINGSKLVGIGPDGSLAKVTIH
                     RKGKPFMVAEGRMVLIEQSHLSSDKAKVVFYIDKDSIYHNGLSFAYHNPTRTVMVNPT
                     DKLTTQSPFYSSYHKINLWSNQLTWSIEKDEITFGSSLGASISKAEFESENFFNQDMF
                     DSMMDASEFHPVLTVWNYTRRIKSNTFLASDLAVHVRRAPEDVKIAMMRLAKLGYVLY
                     NFETDEVTITDKLKYNVMARFGRTDFDVIRFQSTTPGTQPNARLDLNNLNLAIEGVNY
                     ISVSDSQNVFISPYKKSITFQKNRNFEFGGSIRAGLFTFYGKAFRFDYSQFKVELNKV
                     DSLIIDYQTDYRDNYGRRILQGVANALHIISGDILIDKPYNKSGREYNPQYPIFNCTS
                     KSYVYYDASYIYGGIYKRDSFYFEIQPFVYQSMDNFEKADMNFKGILYSGNILAPIEE
                     TLRIRPDNSLGFITTTPSEGMAVYKGKGRVFNKIDLSNQGLFVNGSIDYITSTTQSDK
                     MLLFPDSMVTVSTGFSIAKRETGIEFPNVKGNKHSIKWLPKADKMFISKGEKPFVMYD
                     SLATFSGNLLLQPLGLTGAGLLNVPNGKLSSSLFEYSSNALAANESTLTILKTNTDSV
                     ALETSKVKATINFANMLGEFSRVDKSIYASLDALRYSTYHDRFKWDLKGYKVYFETLM
                     AQEDVLAGKFRVSNMIDRDSIPRGNLFFSTKPAEDSLYFFAPKAVYNTDRIHLVADSV
                     KYIVTADATVFPKNKRVEVDVPNRMYKFSNAKVRANNTTAYHVLHDAEIKINGRKSYG
                     GSAYYDYVDERDSIQTIYFPKVYSDNNMNTYAEGTLTEPDSFRLSPNFAFIGGVSLSA
                     PEKFLFFKGGAKPIYNCPGTIGQWVRFESQINPKRVLIPIGPDPRNLNLHRLISGTAI
                     AEDSLKLYGGLLRSRKFWEDSSLVTATGFMEFNNKNRRFTIAPLYKLDNPDSSGNSVS
                     LQKDYCWAFGEGNVRLPLKLGHVTSQVYGSVIHKLDKNEATIDAIIRLNFHFNQKALE
                     AMAAQLSGAITLDKVDLSRKLYRKMLYDWLTPKELPEALNQLSLFGSFTQMPKSLEST
                     ITLSDVKLRWDPQNKSFVSVGKIGVGTIGNIQVNRFVNGFIEYYKGRFNDRLILYIHI
                     GDNEYYSFYYANGVMFVSATNPEFYNPIASQKSSERRVKPSKGMMGYRYMIGSKRELN
                     RAQLRYKELAFGIKSDIPVDSDDDKPNKEGKEDD"
     gene            complement(59299..59796)
                     /locus_tag="PK049_01685"
     CDS             complement(59299..59796)
                     /locus_tag="PK049_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOK60297.1"
                     /translation="MKAIGILSDTHGFWDKGLTDFFETCDEIWHAGDIGSLELADNMV
                     KLKPLRAVHGNIDDYKTRLVYPEKQLFTTENVNILITHIAGTPGHYDQKVLEILKGNK
                     VNILVCGHSHILRVKYDKQWDMLYINPGAAGNSGFHQVKTAIRLKIDGSNISDLEVWE
                     KKRGF"
     gene            60065..60772
                     /locus_tag="PK049_01690"
     CDS             60065..60772
                     /locus_tag="PK049_01690"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HOK60298.1"
                     /translation="MQIFYAPQIEGDEYRLPEDESKHCIRVLRMQQGDELYLTDGCGN
                     LHTARIADENPKRCLVQIVSTQHEYGKRPFNLHIAIAPTKNIERFEWFLEKATEIGVD
                     TITPLLCERSERKDIKVERLQKIVISAMKQSVKAYLPIINSIQPLRNFVKYTHQADLK
                     AIAHCNSWDLTPFQQVIHRGRSVLVLIGPEGDFTPTEVNLALNSGFTGVSLSTSRLRT
                     ETAGVVACHTANLINGV"
     gene            complement(60977..61177)
                     /locus_tag="PK049_01695"
     CDS             complement(60977..61177)
                     /locus_tag="PK049_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008764408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60299.1"
                     /translation="MGQKKTFVLRIDPEKFAALEKWAADEFRSTNGQIEWIIDQALRK
                     AGRLKKSTLKEKGNQSNENQEG"
     gene            complement(61184..62032)
                     /locus_tag="PK049_01700"
     CDS             complement(61184..62032)
                     /locus_tag="PK049_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOK60300.1"
                     /translation="MEKEFHPKSGYLMLVAWFILVAATISLLITTESPWYALGFLPIV
                     LIMPGFIVVNPNESAVLILFGKYMGTIKRNGFFWTNPFYLKKRISLRARNFNSEPIKV
                     NDKVGNPIMIGLVLVWRVKDTYKASFEVDEYEHFVVVQSDAALRKLAGMFPYDNFEHE
                     EEEITLRAGGEVVNHKLENELIERLTIAGIQVIEARINYIAYASEIAGAMLRRQQATA
                     VVAARSKIVEGAVGMVQMALDQLSQKQIVDLDEEKKAAMVSNLMVVLCSDESARPVVN
                     AGTLHQ"
     gene            complement(62125..62313)
                     /locus_tag="PK049_01705"
     CDS             complement(62125..62313)
                     /locus_tag="PK049_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60301.1"
                     /translation="MKNNNVDSKSRNRPSRKDFEGFNVLEQSNIERYFTDYLEKLERA
                     TPPAPEKQFYQLLAKIKD"
     assembly_gap    62497..62596
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<62597..>64001)
                     /locus_tag="PK049_01710"
     CDS             complement(<62597..>64001)
                     /locus_tag="PK049_01710"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOK60302.1"
                     /translation="GKSKLKPKELHFLKTKGNFDIAAICREANIILRSQLVTKGELLQ
                     YICNALETPEVIDTDNRFKTLTQHLKAKNITSVDSKSNELHGEVSLHKPLSYTIYGAD
                     GIEPGAIEQMENAMSLPVTLAGSLMADAHEGYGLPIGGVLATTGNVIIPYAVGVDIAC
                     RMCMSVFPIKPEILGTKSETLSQILLRTTIFGVGSKNKNHIDTSLFDKKEWSATKVIK
                     QLRDLAYSQLGTSGGGNHFVEWGELHITEFDPLLGFEPGKYLALLSHSGSRGFGSEIA
                     LYYSKLAMEKVRLPGKARHLAWLDLNSQEGQEYWLAMNLAGEYASANHHEIHAKVARE
                     LNVRPIRMIENHHNFAWFESLPDGRKAIVHRKGATPAHPNSLGIIPGSMVHPAFVIKG
                     KGNPASLSSASHGAGRKMSRSKALQTFTQADLNYILEKWGVELIGAGLDEVPMAYKDI
                     DAVMAYQSDLVGVLAKF"
BASE COUNT        17692 a        13226 c        14107 g        18876 t
ORIGIN      
        1 ctccttcaat atcctcttga taatgagatt cctcgcattt gctcggaatg acagggggcg
       61 ttgttccttg ttcatgggtt gaaattgaac gcagatggca cagatttaac tgattacact
      121 gtgaaacact gtgtttaacc ctgtgttact ctgtgaaaca aatacaattg ttcgttgatt
      181 gttgctggtt gttcgatttc gttaaacggt tcacggttca cggttcacgg ttcacgcttt
      241 gtactcattt aacttcctct aacttcctct aacatcatcg cattctgtat actccgttta
      301 acggttcacg cttcacggtt cacggttcca ccattccccg gctaccgatt tctttttctc
      361 ccctttttca gctatcttgc ataggtggta gcgttttgat gttttgtgtt cttaagcatc
      421 tgttataaat gtaacaatgt taatagaata acgtaaattt gtaacggaat tgctattgca
      481 aggatttggt gtaaacacaa aatacggtag gaatgaaaat aggggaatta gcccagctgg
      541 ttgatggtaa tgtggtttgc ggccacgcaa tgctcgatag ggaaattgag tatgcttttg
      601 cctcggacct gatgagcgat gtgcttactg tccgcgctga caaattgttg ctaattacag
      661 gtttggccaa catgcaggcg gttagaaccg ccgagatggc cgatatacca gccattgtat
      721 ttgttcgcaa taaaaccatt agcgaggata tgattcagct ggccgaggat aatggcattg
      781 ttcttatcga aagccctttc accatgttca gggcttccgg cattctttac agtaagggtg
      841 taaaaccggt ttattaatag catatgcact ttgagtacag ggtccaggga ggcgattttg
      901 ttagggccgg gcaagcatcg agccaggtga aaaagatttt aaagcagctc aatgtttccc
      961 ctaacattat taaaagggtg gtggttgccc tgtacgaggg ggaggttaac attgttgctc
     1021 atgcctttca gggtaaaata tatgttgata ttgagaccga taagattcgt gtagtgctcg
     1081 atgatgttgg accaggaatc cccgatattg atttggccat gaaacccgga tactctaccg
     1141 catcgccaat tgtaagggaa atgggttttg gagctggtat gggacttcca aacatcaaag
     1201 agaataccga taaaatgaag attgctagcg ttgttggcgt aggaacacgg ttggagtttg
     1261 aggttaacct taaggagtag cggaggatag ctgtggctga tttggaattc taccatgcgt
     1321 taaaaattct tcccgatgtg tgcatcggct gctcgcactg tatgagagcc tgccccaccg
     1381 aggccttaag ggttgtaaac ggaaaagcta agctaataaa caaccgctgt atcgactgcg
     1441 gtgagtgtta cagggtttgc ccaacccatg ccattacggt tcaacaggac gatttcagta
     1501 aaattttttc ctataaacat cgcattgccc ttattccttc cgttctacta gggcagtttt
     1561 caaaggagat atcacacgat gatgttgcgc aagcattact gagtcttggc tttacgtcca
     1621 ttgttgaggt tgaggattcc gtggaatatt taattggcaa gctgaacagc tacattgctt
     1681 caaccgaggt taaaccggta atatcatcat tttgcccggc aatagtccgg ctaattcagg
     1741 ttaagtttcc ctcgcttgtt gagaatattg ctctgataag accgccattg gaccttaccg
     1801 caacatatat aaagacaagc cttttggaac aaggtgttcc tgaaaaggaa attggaatat
     1861 tctacattac cccttgtgct gccaaaatag ttgctgtgcg acatcccgag ggagaagaaa
     1921 agtcggagat agatgggatt atcaacatgg attttatcta taaccgaatt ctgcgtgaga
     1981 taaaaggtaa attacctacg ggaaatgagc caaaaatcat ttcggatctt agcccccgga
     2041 gttactcttg ggcgctaacc aacggcgaag catcgtgtat cgatggccga tgcctggcta
     2101 ttgatggaat atggaatgtt attgagtttc tggagaaggt ggagaacgat gaaattcaag
     2161 atgtggatta ccttgagctc cgcgcatgcg atgagagctg tgcgggtggt atccttgcgc
     2221 cagggaatcg gtttttagct gccgagctaa ttcgcaaaaa cgcaagtaag gtatcggttt
     2281 ggagtatcaa taaaaccaac gataagatag taaaatacga tgcttgcatt agcggaagaa
     2341 ctgcgctgag cccgcttagc ccaagatcga tgctgaagct cgatgaggat atggcaaagg
     2401 ccatgcttaa gatgcagcgt gttaaccagc tgatgtgctt cctgcccggt atcgactgcg
     2461 gggtttgcgg cgcaccttcg tgcattgcgc ttgccgagga tgttgcacag ggtagcgcat
     2521 cggtttcgca atgcgttttc ctacaaaaaa tcatggagca aaacaagctt gaccctgaac
     2581 actcattccg gatcatgaaa agaatttggg gtgagaaccg gttccgcaag gattgcgaca
     2641 aacccattag gaacaagtaa aaatatcaat atatgacggt aaaagatata gttgatgcat
     2701 taaacctgaa ggtttactcc ggtgaggccg gtttaaacaa ggaggtagtt ggagggtata
     2761 catccgattt gctgagcgat gttatgggga atgcccctgc cggttccgta tggattacgc
     2821 ttcaaactca tgttaatgta atggccgttg ccacgcttaa agatatttcg gctgttattc
     2881 ttgtcaaagg tcttgagcct gaagctaaca ccatggcaca gagtaacaac gaggccattc
     2941 cagtcctggg aaccaacctg caagcctttg agataagcgg ccagctgtat aatctcatca
     3001 acaaaaaatg atagttaagg ccgatttaca tattcactcg gttatatcgc catgtgccga
     3061 cctggaaatg agcccggtga atattgtaat gcgagccgct gaaatgggtc tgaaacttat
     3121 tgcggttacc gatcataaca gcaccctcca tggccctcta atccgaaccc tggctgaaca
     3181 gcatggcatt tactgcctat acggtgccga ggttaatacc cgtgaagaaa tacactgcct
     3241 ttgcctggtc gacactgagg atcagcgcca gttactgcag gcattcatag agcaaaacat
     3301 aacccgcatc cctaacaacc ccgatatttt tggctatcag gtagtggtaa atgaacacga
     3361 cgaaatagtt caggagattg actaccttct acatgcagcc ctggcaaaaa gtatcgatga
     3421 gattgccgag tttgttcatg ggataggtgg ggtttttatc cctgctcatg ttgatagacc
     3481 taagtatagt gttactagtc agcttggctt tgtgccgcca tcgctaagtt acgatgcact
     3541 tgagatttca agaaatacca cagtagattc tttcaagcga aacaacccca taaaacccaa
     3601 cacgcaattc atccgaaatt ccgactcgca ttttcttagc caaataggaa cttcctacac
     3661 agagtataat ctggaaaact tatcgtttaa gtgttttaag gactccatac taaatacagg
     3721 cagttcaacg gtattgcctg tgatatagct ttgtcacttt gtttaaaatc gatataaatt
     3781 aacagttgga aaatgaagga cttatcgctt cacatccttg atattgttca gaactcggtt
     3841 gttgccggtg ccaaggtgat acaaatcatc cttgatatta acaccgcaaa tgattatatt
     3901 gagctcactg tcaaggataa tggcaagggt atggagcaag aatttttgaa gcgggtagta
     3961 gaccccttta ccacaacccg aacaacccgt aaggtgggct taggaatccc attgctaaag
     4021 cagaatgctg aacttaccgg aggagcattc agtatcgatt cagaaccggg agtaggcact
     4081 gcccttaaag cccgtttcgt taagtcgcac atcgattgtt tacccttagg cgatttgggc
     4141 ggcaccattg cgctgctcct ttgttcctac cctgatattg agttcaaggt aagcgtggtt
     4201 gttgatacaa ccgaatttgc ggtgtctaca tcggagttac gccaagcgct tgatggggtt
     4261 ccgttgaacg agccatcggt ttacccgctg gttaaggagt atgtctattc gaatttagac
     4321 gaattacaaa taacgaattt aagctgaaaa acatattttc aattgttaat cgcttaacaa
     4381 ttgaatttaa ttttgcagaa aattattagg ttaatattac gaaaccaacc aaatacacac
     4441 aaggagaaca agttatgaca aaagtaaagt cgcttgccga tctgaaaaag atgaaggaga
     4501 ccctgcaagc caaaatcgac cttagggaaa agggcgaagc tgcagagaac cttgttcaga
     4561 ttaaggttgc catggctacc tgcggtattg cttcaggagc aaaaaatgta atggattttc
     4621 tgcttgagga actggaaaag cgtggaataa acgctgttgt aacacaaacc ggttgcatgg
     4681 gttactgcta tgccgaacct accattgagg taaaattacc caatgccgac cctgttgttt
     4741 tcggctatgt tgacaccaaa aaagccgatg agattattga gaaatacatt aagaacggtg
     4801 aactggttga tggtattatt cccgttaact acgagtcaat tgataacaaa taaccaactg
     4861 ggaggtaata gtaatgtcaa agtataaaat gcacctgcta atttgtggag gcacaggttg
     4921 taaagcttcc tcaagcgatg caatagcaga gaaactaaaa ctagagctgg cagccaataa
     4981 ccttcaggac gaagttcagg ttattatgac cggttgcttt ggtttttgtg agaaaggtcc
     5041 aattgtaaaa gtattgcccg ataatacatt ttacacacag gttaagcctg aggatgccac
     5101 cgaaattgta aaggaacatg tagtaaaggg tcgtaaggtg gagcgtttgc tctatgttga
     5161 tcccactaag aaggctgcca ttgaggattc aaagcatatg ggtttttacc gcaagcaaat
     5221 gcgtattgcg cttcgcaact gcggattcat taaccctgaa aatattgatg aatatattgc
     5281 tcgtgagggt tatcaagcat taggtaaagt gcttactgaa tataccccac agcaaactat
     5341 tgatattatc aaaaaatctg gcttgcgtgg tcgtggaggc gggggattcc caaccggttt
     5401 aaagtgggaa attgccagca aaaaccatgc tgatcaaaag tatgtagtat gtaatgctga
     5461 cgagggcgac cccggtgctt tcatggaccg ctcaattctt gaaggcgatc cacactcagt
     5521 gctggaggcc atggccattt gcggttactg tattggcgct acaaagggtt tgatctacat
     5581 acgtgctgag tatccacttg ccattaaacg cctaaaggtt gccatcgatc aggctcgtga
     5641 gtatgggttg ctgggaagcg aaatctttgg aaccagtttt agcttcgata tcgaattacg
     5701 ctacggagcc ggcgcttttg tttgtggcga ggaaactgcc cttatccact ccatggaggg
     5761 cttacgtggt gaaccaagca acaaaccccc attccctgca caaagtggtt acatgggcaa
     5821 gccaaccaac gtgaataacg ttgaaacctt tgcaaacgta cctccaatta ttttgaaggg
     5881 tgccgactgg tactcatcaa ttggtacgga aaagagcaag ggtactaagg tatttgcgct
     5941 tgcaggtaaa atcaacaacg taggacttat tgaggttcca atgggtatca ccctgcgcga
     6001 ggtaattttt gagataggtg gtggtatcaa gaatggaaag aaatttaagg ctgttcaaac
     6061 cggtggccct tcgggcggtt gcttaaccga gaaacacctc gataccccaa ttgatttcga
     6121 caacctgatt gctgcaggct caatgatggg ttcgggcggt atgattgtta tggacgagga
     6181 cgactgtatg gtatcggttg ccaagttcta cctcgatttc accgttgagg aaagctgcgg
     6241 aaaatgttct ccctgccgta ttggtaacaa gcgtctgtac gagctactgg aacgcattac
     6301 caagggcgag ggtaccatgg aggacctaga gcgtttacgc aacttatcgc aagtaatcaa
     6361 ggatacctcg ctttgcggtt taggacaaac tacgccaaac cccgttctat caaccctgga
     6421 taacttctac gatgagtatg tggctcacgt ggttgataag aaatgtccat cgggtcagtg
     6481 taaatcgctc atgcagtatg ttattgatcc agaggcttgc gtgggatgta cggcttgtgc
     6541 ccgcaactgc cctgtaaatg caatcagtgg cgaacgcaaa aaaccacacg agattaacca
     6601 gtcaatctgt atcaagtgtg gtgcatgtat ggagaagtgt aaattcgatg ctatcagcat
     6661 taaataatta gggtgataac aatggaaaca gtaaaactta caatagataa caaacaggtt
     6721 gaagtaccca agggtactac aatttacaag gcagccagga agttgggtat tgatattcct
     6781 gttctttgct acatggaact ccacgatttg ggagttgaaa acaagcctgc cggatgccgt
     6841 atttgcgtgg ttgaggttga aggacgcaga aaccttgctc ccagctgtgc aactgagtgc
     6901 gctgagggta tggttgttaa aacccatacc acccgtgtta ttaacgcacg tcgcactgta
     6961 atggagttaa tcctttccga ccatccaaag gattgtttaa cctgtcctaa gagtggtcgt
     7021 tgcgacttgc aggatatggc tattaaactc ggaattcgcg agatccccgg tcaggaatac
     7081 gctgagatgt caacatacaa aaaggacttt tctccatcta tcatccgtga tgttgataag
     7141 tgtatcatgt gccgccgctg cgagaccatg tgtaatgagg tacaaaccgt tggtgcactt
     7201 agcgctgtaa accgtggttt tatggctgtt gttgcaccag cttttgagca ggatttggaa
     7261 aaatcgccct gtacctactg tggtcagtgc gtggctgtgt gccccaccgg cgcattaacc
     7321 gaggttgacc ataccacaaa ggttattcgt gctattgccg atcctacaaa gaccgtactt
     7381 gtacaaactg cacctgcagt tcgtgcggct ctaggggagg aatttggtat gaagcccgga
     7441 accttagtta ctggtaagat ggttgctgct ttgcgccaga ttggttttga ttacgttttc
     7501 gataccgatt ttgcagccga cctaaccatt atggaggaag gtaccgagct gctcgacagg
     7561 ataacccgat tcctgaacgg tgacaagagt gtgaagttac ccattcttac cagctgctgc
     7621 ccgggttggg ttaatttctt tgaacacaac tttaccgata tgctcgatgt accatcgagc
     7681 gccaagtcgc cacagcagat gtttggcgca attgccaaat catactttgc tgagaagttg
     7741 ggcaaaaaac gcgaagaatt agtggttgta tcggtaatgc cctgcttggc taagaagtat
     7801 gagtgccaac gcgatgagtt taaggttgat ggcaatcagg atgttgactt ctcaatatca
     7861 acccgtgagt tagcacacct tatcaaggag tttaatatca actttgagga gttgcccgat
     7921 gatgattttg accgtccgtt aggagagtca acaggtgctg gtgtaatatt tggagcaaca
     7981 ggtggtgtaa ttgaagctgc tgttcgtaca gcatatgagg cttatactgg caaacctctg
     8041 cccaagatcg attttacaga gctccgtggt atggagggta tccgttcagc caccatcgat
     8101 ttcaatggat tgccaataaa cattggaatt gctcatggtt tgggtaatgc ccgcaagcta
     8161 ctggaggatg tacgctcggg taaggcaaat taccatgcca ttgaggtaat ggcctgcccc
     8221 ggtggatgta ttggtggtgg cggtcaacca ttgcaccacg gtaactctgc cattatcaaa
     8281 gctcgtatgg aggctattta ccgtgaggat gctggtaaac caattcgcaa atcgcatgag
     8341 aacccctaca tagtaaaact ttatgaggag ttcctaggca agcccatgag cgaaaaggca
     8401 caccacctac tacatactca ttatttcagc aagaaaaagg atatctacat taaataggga
     8461 ggaaacacaa atgtcacata taaagcttga gcttaaaccc gaacaggtag ccaagattaa
     8521 ggaaatatgc aagtcgttta acaacgaccc gggcgaactg ataaatgtat tacataagac
     8581 tcaggaaacc tttggttacc tacccgctga ggttcaggaa gtagtggctg ccgagttaaa
     8641 catttcggtt gcaaaggtgt atggtgttgt aacgttctac tcgttcttca ctatgatacc
     8701 taagggtaag cacccgatta gcatttgtac aggtacagca tgctacgttc gtggggccga
     8761 aaaggtgctc gatgaattca aacgggtact taacgttaag gtgggtgaaa ccacacccga
     8821 tggcaagttt tcattaaact gcctgcgctg tgttggtgct tgtggacttg ctccggtagt
     8881 gctgattggt gaaaaggtgt atggccgtgt ttcacccgat ggagttaagg atatcattaa
     8941 ggagtacgaa gatcaataaa cagcataatg cctgatttca ggtattagtt taggttggtt
     9001 atgtttaggg gtttacattt tttgtaagcc cctaactttt tttgtatttc ttaacgcaag
     9061 tttaattaaa cctgtgtgtc aaatcggtgt ttaattaatg ttgaatttta ggaataaact
     9121 atgatgaaaa aggttttact gctttgggct attgcagttg ttacagtaac ctccatggct
     9181 caggatagaa tgcagggccg tagtggacag aattttgaca tgagcaactt taacggggtg
     9241 attaccggaa aggttatcga ctcgctagat agaaatggtg ttgagtttgc aaacattgcg
     9301 ctctataaac agaaggattc ctcacttgta accggaaccc ttaccgatgc atcgggtaat
     9361 tttatgatgg aaaagctgat gcccggccgg tactatgttg acgtaaaatt tataggctac
     9421 cagagcagaa ggataagcgg aattatggtt tcgccacgta gccctaaggt cgatttggga
     9481 actttctcat tactggcagc cagtgagaat atcgaagcgg ttgttgttac cggcgaaaag
     9541 aaaatgctgc tccataacct tgataaaaag gttttcaatg ttgataagga tatagcggtt
     9601 gagggaggaa ctgcagccga tgtactacag aatatcccat cggttgaggt cgataccgat
     9661 ggtaatgtaa gcttaagggg cagctctaac gtcaatattc tcattgatgg ccgtccatcc
     9721 atgttaaaca gcatggagga attacctgct caaatgattg atagggttga ggttattaca
     9781 aatccctcgg ccaagtacga tcccgatgga ataaccggta ttatcaacat agtgcttaaa
     9841 aaacgtaagg aacctggtta taatggaatg gtaagcttaa atgctggaac cggcaataag
     9901 tataacgcaa ccgtaaacat gaattggcgt caggataagg taaacgtgtt tgccaattat
     9961 agttttcgta ggggtcaaat ggataggttt tccattggcg atagggtaac cctctttaac
    10021 agtggtgccg actcctcatt cctttcgcaa aactccgagg gacaatcgaa catgtttttt
    10081 cacaacatac gtggcggtgc cgactatttt attgactcca ggacaaccct ttcgttcacc
    10141 ggaaacttaa atttccgttc gttcgacatg gataataatg tattatcgaa tagctactct
    10201 aacttcaata gtaacaccct acagaataca agaatttcac taaactccaa cgatggcctt
    10261 gggcatgagt actcgctcaa cttcaagcga acatacgata ctcctggtaa ggagtggacc
    10321 gccgatgcgt tcttttcgcg taatactaac aacaacctaa ataatatcaa ccagcaggag
    10381 atttttaata ccgtacccac tactgctctt gaacgcgccg aaaccgacgg gtggatgaac
    10441 acttttacct ttcaaaccga ctatgttaca ccatttggga atggaggaag gatagagaca
    10501 ggaataaaag cacaaataag acgaagcgat gctgactacg tgtataaaat cttcaattca
    10561 ttaggcagta catgggattt tgacgctaac cgctccaatc actttgttta caacgaggag
    10621 atttattccg catatggaat ttattcaaac acctttgcta acggaaagtt tagctaccag
    10681 ttaggtttgc gggttgagga ccaacactca aaatcagacc agagaacaac caatgaggtt
    10741 gccgatgtaa ataggttgaa cctgtttccc agtgcgcata tccgttggga accaaacccc
    10801 atcaacagcc ttcagctatc atacagccgc agggtaaaca gaccaagcgt gatggtgtta
    10861 aacccatttc taaatacttc cgataatttt aactggtcga agggaaatcc ctaccttgag
    10921 cctgagttta ccagctcaat tgatttaagc cataacctca acttcccaag gacaaagatt
    10981 accacatcgg tttactaccg cgatacacga aatggcttct ccaggcgaat gattgttatt
    11041 gataccgtgg caacacaacc tacacttact accttcatta acctgtcgca ctatgagaat
    11101 attggtgccg aggcagttgt aacacaaaac attgctaaat ggtggagaat taacgctagt
    11161 tacagctact actactctaa gctgtttggc gatgtggtta gcggagctaa cgagggaagt
    11221 tcctggaacg ctaagctggc ttcgtttttt accattggaa agaatgttga tattcagttg
    11281 accggaaact accgtgcacc ctcaattact gttggcggtt caggccgagg tttctatatg
    11341 gcaggtggcg cacagggtga aaccaaggag atgtactggg ttgatttagg tgcacgcatc
    11401 aatgtgctaa ataaaaaggg aactatcaca gttagggtga gcgatatttt taatacccaa
    11461 aaaatgaagt atagttcatg ggatacaaac ttttactcgt ataacgaaag ctggcgcgac
    11521 agcagggtag tgtttgttgg attctcatac agaattaaca actataagat gagacctgag
    11581 aaaaaggtcg attccgatga ttcttttgat atggttgaat agtttattct ggtttaggtt
    11641 ggatgaaggc cgcttgtttt gagcggcttt ccttttagtt taaaccacct ttcccacggt
    11701 aaagctttat tggctcatca aaatcaatcc taaccacggt cattaaggcg tcagttagct
    11761 ccttgggaac atcaatgaag atgagtcctg gaaccggact ccatgaaatt ttaccaacaa
    11821 ctttatgaag tagttcatgg tttgttccca aaactgttgc cttttttatt gggctgttaa
    11881 ttcctttcag catgatttgg ccataagttt tggccgggag gaatagaaat agcgatgttg
    11941 aatcctttga aagggtagta gcgccataga agtgccccat aggcaaaccc gggagtgtgc
    12001 cataaattgc atcggaatgt ttaccaatcc agtttccaat ggtgctaagg gttttaacct
    12061 gttcttccgg aattgagcca tcggcttttg gggtgatatc gagcaacagg tttccaccca
    12121 tgctgatagc atcggtaagg atgctaacta tttcaaaggg agttttatag attgtatcgt
    12181 ttacccggta tccccaggag ctattcatgg tcatgcaaag ttcccagtat ttcaggttag
    12241 gccttgttac cggaaagttt tgctctggcg tatcgtaatc gccataaccc tgtaatcggc
    12301 cattaataat ggtatttggg ttgtgcctat gaataatttc tctaattttt tcggcttgcc
    12361 actccttggc cgagtgctcc cagtcgccgt cgaaccagat taaatcaggg gcgtagagct
    12421 gcataatctc ttcaatttgc ccgtggcaaa aatttaggta ttgttgccac cttagggtgt
    12481 catcggatac attgtagcgg ttcgagtctc tagtaaatcc ggggtaatcg gggtgtgtcc
    12541 aatcaattaa cgagaaataa attccaacct tcaatccttg ttcccgtaag gctttaataa
    12601 atggcgataa aacatcgcgg gctgctggcg attgcgttgc catggagagg ttgctgagtt
    12661 tggtatccca caatgcaact ccatcgtgat gcttagctgt gataacaacg taccttgctc
    12721 ccgactgcat aactagcctt gcccactggg caggatcgta gcgctccgcg ctgaacaggt
    12781 ttatttgtgc catatagtcg gcatgcgata cctgtttgtt gtaaaaagcc caggattcag
    12841 atgtatttcc caacgagtat atgccccagt gtatgaagat tccaaatttt gcatcggaaa
    12901 accattgcat acgatcttcg ctcccctggg agtagcaatt aattgtaaaa atcaaaagga
    12961 gtggtaagaa aaagtttctt gctgtatttg tcagagcttt cattttaaac gctttatgtg
    13021 atacgttaga atctgttaat cgattaacaa gtaaataaaa gtatctatat ttttcaatat
    13081 gaaatttctt ttattcattt gcacccaaat ttaaaaagta taatgctatg tttttgttaa
    13141 tggtagttct ttttgttcta ggctatgcag ccatagcact tgagcacaat atcaaggtaa
    13201 acaaaacagc atcggcgctt tttcttggtg ttgccttgtg ggttttgctt atgctcgatg
    13261 ctggaggaat tttaggtact gatattggtg gaaggtttgg ggagtttatt aacagtcatc
    13321 ccgaattggc atcgttgcca aaatgggaac agtatgttaa ttatattgca gataatcaga
    13381 tacttgggtt tttgggagag atctctgaga ttctattctt cctaatggga gcaatgacta
    13441 ttgttgagat tgttgaccag catggtggat ttaaaataat aaccaatagg attaagaccc
    13501 gcaataaagt taagctcttg tggataatat cgtttgttac ctttttcctc tcggcagttc
    13561 tggataatct tacaacctca attgttatgg tggccttgct tcgcaagctt attgatggca
    13621 agcacgatag atggttgtac gctggaatgg ttatacttgc tgcaaacgca ggtggggctt
    13681 ggtcgcctat tggcgatgta acaaccataa tgctttggat aaagggtaat gtaacaacgg
    13741 ctggtattat tccaaagctt ttctttgcta gtttagttag cttaatagtc ccacttgtag
    13801 cactttcatt tacacttaaa ggcgattttg cccagagcct cgattctagc tcagaaaagg
    13861 ttcattcagt aactcctgca acccgtcggg agcgccactt aatctttatt ctgggtgttg
    13921 gtgctttgct gttcacacca gtatttaagg tgttaaccca tttgcctcca ttcatgggaa
    13981 tgcttttagg tttatcggtt ctttggatat tcactgaggt gatgtatcac cgtaagcata
    14041 accttgacga aagggagaaa cgcacagtag ctcgtgttat aaagaatgta gatgttccta
    14101 ccatactgtt ctttttgggt atccttatgg ctgttgacgc cttacaaaca gccggacatt
    14161 taaccttgct ctcgcaatgg ctcgataggt cattcacaag tccctatcca ccaaacattc
    14221 ttataggtct tctttcatcg gttgtagata atgttccact tgtagctgct gccatgggca
    14281 tgcaccctgc ggtttcgccg gatttggtaa acactgcaag tgcaataagt tcagcctatg
    14341 tggctgatgg tgatttttgg aatcttttgg cttattgtgc tggtactgga ggtagcattc
    14401 tgattattgg ttctgccgcc ggggtggctg taatgggact tgaaaaaatc gattttatgt
    14461 ggtatttaaa acacatcagt ttactagcat tacttggata ctttgctgga atacttacat
    14521 attttttgat gtttgggtaa tactatgtat tgactaagca aagtccgtag tgagaaattt
    14581 cactgcggat tttttgttta tcactcaaat aatttttaat attgtgggtt gtattctaaa
    14641 attacttaaa tgagcattca aatagttaag gtagagaaca aaacggatct ccgaaaattc
    14701 attcacttac cagcaataat ccataagggg cataagaatt gggtacctcc catttactct
    14761 gatgagtggg atttttacaa tcccaggaaa aacagagcca tgtcgtattc cgataccgtg
    14821 ctatacctcg ctttaaataa tggtaggcct gtaggacgaa taatggggat aattaacagg
    14881 cgctataacc aaatccataa cgaaaaaaca gcccgctttt tcaatctgga aacatttcag
    14941 gattacgatg ttgcgcaggc acttttggga gaggttgaga agtgggcaaa ggaaaaaggt
    15001 atggataggt taattggtcc aatgggtttt tccgataaag accctcaggg tttactagtt
    15061 gagggtttcc atgaaccaat tgttctggca tcgaactgta actaccccta ccttgttgaa
    15121 tttgttaccc gatatgggtt tgagaaggac attgatttgg ttgtatacaa ggttcctgtt
    15181 ccccatgaac tacccgactt ttaccttcgc atacttcaac gggttaaatc ccgagcaaac
    15241 ggctttagaa tgcttagttt ctccaaaaga agcgatatca aaccattcat taaacccgtt
    15301 ctttcactgg ttaacgaaac ctttgttgat atctatggtt ttaaccccat ggattccgat
    15361 gaaatggagg agcttgctgc aaaatacctt tcatttatcg atccacgctt tttgaaggtt
    15421 atagttaata cccacaatca acctttggct ttcatacttg gtattcccga tatatgtccc
    15481 ggtattcaaa ggagtaaggg ataccttttt ccattagggt ttattcatat cctaagagca
    15541 atgcgaacct caaaaacgct aagtcttttg cttggtgcca ttcatccgca gtataggaat
    15601 ttaggatttg atacctggat gggggccgaa atgctacacg aggctcagaa agcaggtatg
    15661 actattattg atagccacct tgagctagaa acaaaccgta agatgcgtgc cgaaatggaa
    15721 aaaatgggcg gtgttgttta caaacgctat aggattttcc agaaaaaact ataaaaaaca
    15781 aggggttgca aaatgcaacc ccttggctat tttacaagat tgtgttgaaa agaattttat
    15841 agtttgatgg aaccgatgcc ctgaatattg ggctaaaacc aagcaaaatt acttgtcctt
    15901 tccctttcct gagccatatt gctgcggtca tgccatccat tatctcctcg cctttaaggt
    15961 aaccgcttag gagtttactc tttccttcaa agtatccaat tactcttcga tccatgtcga
    16021 gcgaaggaac ttgggtttca aacaagtagc tatcgcgcat gaaaattccc aactcgtcag
    16081 caagtccaaa agtgaatggg taaacaggtt ttatctttaa tctgactaag gagccaggac
    16141 aggaaaagcc ttgcttttga acttcctttg cgttattctt aaagggtaac ataaactcct
    16201 cggaaacatt accctgtttg agggataacg ttccctcaaa cagttcagtt gcttgctccc
    16261 aagctaaaac ggttcctcca ttttgaatga attcggttaa ggcctcaatc cccttctgtc
    16321 ccattccctt ggtataaatt tcggggtaaa ccgatggaat aacttgccca ttcctcttgt
    16381 atttgccatc cttgagcagg gttgcgttgg tgtttggaat aataaccagg tcgaatttcg
    16441 atagtaagtt gccttcggca acctgcgaag gatttataac agtgtatgga atttcgtagg
    16501 tgtcgagaat atacttagtc caacctgcat ccatgctttg aacctgcgat tcaagtaaac
    16561 ctatccgacg tatcttgctg atgctggtat tgttgggttt gacatctgaa gtcattatga
    16621 taaaattcgc atcgttaagt atttgtctgg ttttttcatt caggggaatg cagaaacttc
    16681 ctgctggtaa ttcaatgcca tcgaacacag tatgttcgtt agttcgctcc acctgaacgc
    16741 cactctttag gcatttaact gccaatttat aggactggtt tactgttgaa ggaagtatag
    16801 caaagcctat ttcgggcaac gttttttggt ttagtgtgaa gcttgattgt atgggttcaa
    16861 taatgctgtt gtcaatagcg ttgttattta tggtaaaaca ggttaaaccc ttatgcaatg
    16921 gtaacgacca actggttata tcgtatggtt ccaatacttt tccgccctcc tctaaccgcc
    16981 gttcagggaa ctcctggggt tccatcacct ctttgataaa catccggaag ggctgtgcaa
    17041 gtggaacaac aatatcgcca gaaaggaaat tttgattctg tatagtgata ttttgcttta
    17101 accggtaaac atttacgccg tgctctttga gcaagtttac taacgaaaca agctcgctgg
    17161 gatcctgctg attggctgga agtatataat ggtagggagc gattgttttg cccaggctta
    17221 cctgtttttt gcagatatcg tttcggaaca ttagaatacg ttccttgtac ttgctggcag
    17281 tttgtagcat tgagatggtt gactcaactt caagcgcaac aatgtccgat aaatgccacc
    17341 agccgccagg ccatggtaaa agcatgttta cacctttttt atactcagcc aaccctttcg
    17401 aggtgccggt tagctcagtt ggttcaatat atacgggagt ggcaactctg gcactggctg
    17461 cctcggttaa catgcttatt acatttttcc acaaacaggt ttcagttgag cccggccagt
    17521 agttgtcaaa aagattttgt tggctaacgc cctgcaaacc cttggaggta aggtcgttta
    17581 gcatgttttg cccaaaaagg ttcgaccaag cgtaaagttc tgcatcaaca ttttgggcga
    17641 ttgggtcgtg gtagggcggt acaaagtacc ggggacccgt tagtcccatc tggtgcttct
    17701 caaccataac ttgagggaac cattcctggc tggttagcct ggatatggct tttgtttccg
    17761 gctgggttag ggtaataaaa tcgcggttgt tgtcgtgtcc aacatacttt tggtaaagaa
    17821 atggcattgg gccaccctcg tattttgatc ctttgaattg gttatagtaa tcaacaacca
    17881 tgtccatccc gtcggggttg tggcaaacaa ctgccatgta aacaacgttg ttaagagcat
    17941 tgctgaacgt atttccagaa atcaacctgt aaattattgt tggaagggcc tgggtaggcc
    18001 caacctcagt ggaatgcatg gagagggtag ccatcacaaa aaccttgcct tgagctacca
    18061 tttgttccct ttcagcattg tttagggaat agttaatggc taactgtttg ttaatctcct
    18121 taagcctatt taggttagcg atattctctt tactgcttac aaataccgca taaagatttt
    18181 ttcccatagc tgtttgacca attggtgtta gcttaaccat tggactttgg gtttcaacct
    18241 ttctaaggta ttcaacaact tgatcgtact tgaaaagcat acgatcggct ccgggcgtga
    18301 aaccgaaatg ctcttccggc gatgttatta cctgtgcatg tgaaaacaat atcccaatag
    18361 taaaggataa aagcaatgtg attgcacgtt tcatactatt ttgatttagg ttggttactt
    18421 ccctttgact atttggtttt caaatggttt acaagtaaaa aagccacgag ttttatcgtg
    18481 gcttttaaaa gtattatgct tgcgattaaa aactcttaat acaatcgtca acgctttccc
    18541 ttatatcgaa tacggtttga agttgtaata gcttgatgag ttccattact tcagggctga
    18601 tgttgcatag ctttaaggtg gcgtttgaat ttttacaatg acggagtatt gatagcaaca
    18661 tgccaaaacc tgtgctatcg atatagctaa caccgcttag gtttaatgct acctttgcat
    18721 ttggagtaat cacttttata acttcagttt taatcttttg tgtaacggtt acattcagct
    18781 tgctattttc gctgaacgag atcaccatta catcgttaat tttttcaaca tcaatcatat
    18841 atgtaagttt ttaaagttta tacttataac gattcaagaa tatgttgtag ttcaacagaa
    18901 gcctcgcctg atattttttt aaacctttcc acgtaaacct ttacagtgtc atcgttgggt
    18961 ttgctggtgc aaatgttttc aagttttttc atatcggatg taagctcatc aattcccatc
    19021 atggctaccg agttcttaat tttatgggcc agttttgcca gggtgcagta atcctgattt
    19081 ttaaggtaga tttccatttg ctcagagaag gtgggtacct gggtaatgaa aaggttaatc
    19141 aaatcgcgca ttaaatcggt acttccttcc gaaatctctt tcagaaaatc gaggtttaca
    19201 tggtgggttg cctgatcggt agccatcttt taaaaagatt gagaatacta ctactttcaa
    19261 atttactaaa aaaatatgat atgttgaaaa acataagcat aaaagtttac atcgaaatcg
    19321 gtttaaataa caagaaacaa ataaaaatca tagatttgcg gtactgtaaa taaatacata
    19381 aaaccaatga aaaatacacc atttacaaag taccatatag agcttggagc ccgtatggca
    19441 ccatttgcag gctataatat gcccattgag tattctggta ttaacgacga gcacataact
    19501 gttcgtgaga aagttggcgt ttttgatgtt tcgcacatgg gcgaaatttg ggtaaaaggc
    19561 cctaaagcgc ttgattttct tcaaaccgtt accagtaacg atgtttcagc attattcgac
    19621 ggtaaagttc agtatagctg tttcccgaat gataagggcg gaatagttga cgacctttta
    19681 gtttacaggg ttaatgctga aacctacctt ttagtggtga atgctgctaa tattgaaaag
    19741 gactggaact ggctttgcga gcagggtaaa aagtttggtt taactcccgg taaggagtta
    19801 tataatgctt ccgacgaatt tgcgcagctt gccattcagg gtcctttagc cttgaaggcc
    19861 atgcaaaagt taactacaga gaatgtggtt gatatggaat accacacctt taagaaaatc
    19921 ccatttgcag ggattaagga ggtaatattc tctactacag gttacaccgg cgctggtgga
    19981 tgcgaaatat actgcgccaa cgaggatgcc gataaactat ggaaggcagt ttttgaggca
    20041 ggcgctgagt atggcattaa gccaattgga ttaggcgccc gcgatacgct ccgtctggaa
    20101 gccggtttct gcttgtatgg aaacgatatt aacgattcta cttcggccat tgaggcaggc
    20161 ttaggctgga taaccaagtt tgtccccggc aagaacttca ttagccgcga tgtatgggaa
    20221 aagcataaaa acgaaggaac cgatcgccgc ctgaagggct ttgtgatgat tgatcgcgga
    20281 ataccccgcc aacactactt gatttacgat gctcaaggta acgaaattgg cgaggttacc
    20341 tcaggaacca tgtcgcctat gagtaagcag ggtgtaggta tggggtatat caaaaaaggc
    20401 cactggaacg atggcaatga gatttatatt ggtatccgcg ataagaaatt aaaggctaaa
    20461 attgttaagc ctccctttca cttagcaaaa gtataaaccg taaaaggggg cacagccccc
    20521 tttttttaaa tcaaactata ccatgccaag gattgaagtt gcattttcgc ctaccatctt
    20581 tcagcactat gataaagaag gtaaaatagt tgtggtggtt gatatcctca gggcaacctc
    20641 agtaatttgc accatgtttc acaatggggt taaggagata attcctgtgc gaagtattga
    20701 ggaggctcat gagtataaac gcaatggcta tcttgtggtt gctgaacgca atggccaaaa
    20761 gctcgacttt gccgattttg gaaattctcc attttacttt accccagaaa ctgttaacgg
    20821 gaaaactatt gtatatagca caaccaacgg aacaaatgca attactttag gacgtgatgc
    20881 ccaggaggtg gttatagcct cgtttttaaa tatctctgcc gttgaaagtt accttaacgc
    20941 ccgctcaagc gatatactta tcatgtgtgc cggttggaaa ggcaagttct gtcttgagga
    21001 tgccattttt gcaggagcat tggcccaaag gcttttaacc actggccgtt tcgagaccat
    21061 atgcgattca accaatgctg ctattgatct ttggaatgtg gctaaagtag atttaaccag
    21121 ctacgttgaa aaagttgctc agcgacatcg ccttaaaaag ttaggcttgg atgatgttat
    21181 tggttactgc tttacctccg atgttaccga tacaattcca gttctcaaag aggataagtt
    21241 ggtgccttta atctagcatt gcaaacgatt gttaaaaaac gattcaaact tcctgtttga
    21301 taacatttat ttaggaaatt tgaaaaaaat ctttgaaatt tgacctattc ttgcaataac
    21361 agttgaaaac ttaaaccatt taacgccatg aaaaaacata atttcaatgc aggtccctgt
    21421 atcctgcccg aagtagcact tgaaaacact gccaaagcag ttctcgacct cgatggtatt
    21481 ggcatgtcaa tcctgaccat ctcgcaccgt tcaaaggagt ttgaggcaat tatggaagaa
    21541 accaaagcac tctggcgtga attgcttcac attccagaaa actaccatat tcttttcctg
    21601 ggaggcggtg caagcttgca gttctgcatg gttccataca acctactcca aagcaaagcc
    21661 gcttaccttg ataccggggt atgggctaac aaggctctga aagaggccaa gctttttggt
    21721 gaggttgtta ccgttgcttc ctcatccgat aagaattaca cttatattcc aaaagggttt
    21781 accattccaa ccgatgtgga ttacttccac attaccacca acaacaccat atacggaact
    21841 gagattaagc atgatatgga ttcgccggta accctggttg ccgatatgag ttccgatatc
    21901 atgagccgtc cggttgatgt taaaaagtat gggttgattt acggtggcgc tcaaaagaat
    21961 gttggccctg ccggcgttac ctttgttatt atccgtgagg atatcctggg caaagttgag
    22021 cgcaagattc ctacaatgct cgactaccgc acccatatta aggatggttc aatgtttaac
    22081 acacctcctt gcggtgctat atacaccatg cgcgaaaccc ttaaatggct taagggaatt
    22141 ggaggcgttg aaaaaatcaa cgagctaaac aagaagaagg ctcaactact ctacgatgct
    22201 attgacaata gcaaaatgtt tgttggaact gccgaaaagg attcgcgttc tatcatgaac
    22261 atttgctttg ttatgaaacc tgagtataag gataaagagg atgctttcat ggactttgca
    22321 aagaaggcag gaatggttgg tattaaaggt caccgctctg tgggtggctt ccgcgcttca
    22381 acctacaacg ctttgcctat tgaaagtgtt caggctctgg ttgactgcat gaaggaattt
    22441 gagaagcaaa atgcctaatt taattcagat gggttgaatg ggttcattcc tgttcagccc
    22501 atttttttag aattaaggtt tcatcatttt ctctctttaa gtatttttca aattaataag
    22561 ccattttcca tgagccttca tcaaactttg ctggaaggaa ttgtgaaaaa tggattttgg
    22621 agcaaaaggt acccaggtat ctatagggct ctatcgcaaa atgctaggat atttcggttt
    22681 ttatcgatgg gtttaggttg gataggtata gttctgtgta taacttatta attagaagaa
    22741 agcaaaatgc ttgattcatt tatgaattcg acgcgaaacc gaagtaacct aaagaattaa
    22801 ggagattcat gagtttgata tcctaattct gatttctgac caatgaacca ataaagtaac
    22861 tcaaaactta aaatctatga caaaagtact agtggccact gagaaacctt ttgcaaaggt
    22921 tgctgtggaa ggaatacgaa aaattgttga ggatgccggg tttgaattgg ttttgcttga
    22981 aaagtacacc gatcctaacg atttggttaa ggctgtagcc gatgttgatg cacttatcgt
    23041 acgcagcgat aaggttacca aggaagttgt tgatgcaggt aagaacctga agattgtggt
    23101 tcgtgccggt gccggttacg acaatctcga tttacctgct tgcactgcta agggagttgt
    23161 ggcaatgaat actcctggtc aaaactcaaa tgcagttgct gagctagcta ttggcatgat
    23221 gatctatatg gctcgtaatc ttttccagcc cggaacaggc actgagattg ctggtaaaaa
    23281 attaggtatt cacgcctatg gcaacgtggg caaactggtt gccaagtacg gaaagggatt
    23341 tggcatggag gtttatgcct ttgatccttt tgttgataag gcagtaatgg aaaaagacgg
    23401 tattaaggtg gttgatagca tcgagatgct ttactcctcg tgcgactatg tttcgcttca
    23461 cattccggcc aacgataaaa caaaaaaatc aattggctac gacttactga gcaaaatgcc
    23521 caagggtgca acccttgtga acactgcccg taaggaggta atagacgagg atggcctcaa
    23581 aaaggttctt gctgagcgta ccgatttaaa gtatgccact gatattatgg ccgactgcca
    23641 tgctgagctg gttgagaagt tcccgggcag ggtatttgcc acacccaaga agatgggtgc
    23701 tgaaaccgcc gaggcaaaca ttaacgcagg cttagctgct gcccgtcaaa ttgtggcatt
    23761 catcaagaat ggtgataaaa cttttcaggt aaataagtag ttggtacgtt aggcgttaaa
    23821 cgttaaacgt tatccgaatt aattaaggga tggaccataa ggaattagat gcttggaaag
    23881 ttgccgttga tttatcagtt gagatatatt ccttaactca gagttttcca aaagaggaaa
    23941 tttttgggtt ggtccaacag ataagacgtt cagctatctc gatcccatct aatatcgctg
    24001 agggatgcgc taggggtagt tcaaaggaaa ccatacattt cctttatgtc tctcttggct
    24061 cattggctga attggaaacg caaataataa ttgcttcaaa attgggcttt gttaatggaa
    24121 tcgatgaact tttaaaaaag ttgaacaaat taaagcaagt tattttagga ttaattcgat
    24181 atttgaaaac cgtttaacgt ctcacgttta acatttaacg tttaaattat ggtaaaaatt
    24241 aaagcattta agggtattcg tccaccaagg gagattgcaa aggaggtggc tgcacgtccg
    24301 tgcggcgtgc ttaactcaca ggaagcaaaa gctgaagcag gggaaaaatc gttgttgcat
    24361 atcactaaac ccgaaatcga ttttgatcct ataatcgatg agcacaccca acaggcttac
    24421 gataaggcgg tggagaactt ccgcaaatgg aaggaacgtg gctggttggt tcaggacccc
    24481 aaggatatgt actacgtgta tgcccaaacc atggatggta gaactcagta tggcctggta
    24541 gttgcttgcc attttgagga ttacatggag ggacgtatca aaaagcacga gttaacccgt
    24601 cccgacaagg aagaggatag catgattcac atccgaatcc aaaacgccaa cattgagccc
    24661 gttttctttg cttaccccgc tcataaggag atggacgaga ttgtaacaaa cgtagtgaca
    24721 agcaaaaaac cggaatacga ttttgttgcc gacgatggct ttggccacca tttctgggta
    24781 attgacgatg aggcaaccat taagcgtatc actgaaatat ttgcatcaat ccctgccctt
    24841 tacgttgccg atggtcacca ccgtactgct gctgcagctc gtgtgggact tgagaaaaag
    24901 aatgccaatc ctaaccaccg tggcgacgag gagtataatt acttccttgc cgtaatattc
    24961 cccgataatc agcttaagat tattgactat aaccgtgtag ttaaggattt gaatggtctt
    25021 actcctgaac agttccttca aaaacttaac gaaggtttcg tggttgagga aaaaggctca
    25081 gagatttaca agccgcagca actacacaat ttcagcatgt acctggaagg taagtggtat
    25141 tccctaaccg ctaaacctgg cacctacaac gataacgatc ccattggcgt tctggatgtt
    25201 accatcctgt cgaaccttgt gcttgatcct atcctcgata tcaaggatct aagaacctca
    25261 aagcgtatcg attatgttgg cggcatccgt ggtttaggtg agcttaaaaa gagggtggat
    25321 agcggtgaga tgaaagtggc ttttgcactt taccctgttt ccatgaagca acttattgat
    25381 attgccgata caggcaatat catgcctcct aaaaccacct ggtttgagcc taagctccgc
    25441 agcgggttgg taatccacgc tttggaatag tgtaaaaatg aaatttttaa aagactcagc
    25501 atttgctgag tcttttttaa tcgccatagt tcaacaatcg ttttcaaaat ggtataacct
    25561 gtagtggagc taagccaaaa gtgtttattt ttgcattaaa cactatgtaa tcatgagcat
    25621 tcctgagcaa cttgaaaaaa tcaagaatga attaccacag catgttaagt tgattgctgt
    25681 aagtaaaact caccccgtga gtgtgattat ggaggcgtat aatgccgggc ataaattttt
    25741 tggcgaaaac agggtgcagg aacttatttc aaagtatgat gaaatgccta aagatattga
    25801 gtggcattta atcgggcacc tacaaaccaa taaggtaaaa tacattgctc cctttgtgtc
    25861 gcttattcat tcagtcgata gcttaaagct gctctctgta attgataagg aagcgcaaaa
    25921 gtgcaatagg gttattgact gcttgttaca ggtttacatt gcatcggaag agacaaagtt
    25981 cggcctatcg gccgatgaac tccatgaact acttcaaaac caagagtttg aacagttaca
    26041 aaatgttagg gtatgcggcc ttatgggaat ggcaacattt actgacaata tggaacaggt
    26101 tcggatggag tttaggtttc ttaagaagct gttcaacgag attaaacaaa cctacttcca
    26161 gaatcaaccc tggtttaatg agctatccat gggtatgtcc agcgattacc gcattgcaat
    26221 tgaggagggc agcaccatgg tacgaatagg aagtaatatt tttggacacc gaagctatac
    26281 ctaaactaaa tttttgtaaa tttcaatcgt attcaaaaac ctttaaccat ggctaaacta
    26341 gaaacatcct acttggggct atcgctacgt tcaccgttaa ttgtcagtag ttctgggtta
    26401 aactcatcgg tcgatcgcat taaaaaatac caagaatttg gtgctggtgc tatagtgctt
    26461 aagtcgttat ttgaggagca aatcctccat gaggtaaatc acctggaaaa gtattccgat
    26521 tataccgaag caaccgatta cctgaagcac tacgtatcgg ccaacaatgt tcaggagtat
    26581 ataaacctaa tcaaaaatgc aaagcaatcg gttgatatcc cagtaattgc tagcataaac
    26641 tgcctgagcg ataaccaatg gctatcgttt gcctcacgta ttcaggaggc tggagccgat
    26701 gcgcttgaag taaatattta ctacttgccg ctcgacccta agcgtaaggc tgaggagtat
    26761 gagcaggttt acaatagcat tgcagagaag ctgcaaaagg ttataacaat ccctgttgca
    26821 tataagttag gatcgcactt taccaatccc ttaaatgtgg ttgagcacct tcgctaccgg
    26881 ggagccaaag gggttgtact ttttaaccgg ttctttgagc ccgatatcga cattcatgca
    26941 atgaaggtgg tacccgccga cattttcagc aatccatcgg atttgcgata taccctaagg
    27001 tgggtagcca tagccagcca tttgattcct gaaattgaca tatcggcttc aacaggtgtt
    27061 cacgatggcg aggccttggt aaagatgcta cttgcaggcg ccaagtcggt tcaggtttgc
    27121 tctgctattt accgaaacgg gcctaagcat atagaagcta tgctcaatac ccttacagaa
    27181 tggatgaacc agaaaggctt taaaacgata tccgatttcc ggggtaagct taatgtaggc
    27241 tccattgccg acccaacgca gtacgagcga agccagttta tgaagtattt ttcaaactac
    27301 gaataaaaat gaaagaaccg ccaactggcg gttctttttt atagttcatc cagtaatttc
    27361 accaactctt caattagggg tttagtttca gcgttatccc ctttgacgga tttgattttg
    27421 gctttcaaat cgttaagttg ctctccgctc aaattctcgg tcatgttttc aatcacggta
    27481 aaagcctcga ggcaagcctg gtaattgccg tgttccatta tgtcgataaa agtatcgacg
    27541 taaggagtaa agtccaggcg gctttgccag caaaccgaaa ccaacaggtg aatatctttc
    27601 gacctcttgt tttgccttat tgcctcaact attgtatcaa tactcgattt gttttttagg
    27661 tcaaccagga agtttacaac ctcgttcttc aacttttccg aacgcgtgct gaaaagcaag
    27721 ctgagtattg ttggaagcat atagtcctca ccggtttccc tcaattcagt aagcaaacca
    27781 ataattgcct cgtcggaact cgcttcaata gtcttttgga actgttctgc cttgttgggc
    27841 tgcatagggt ttaaattttt ttacaaaggt attgcttttg gttttacttt ggttttaaaa
    27901 tcatttgttt acctaaaaaa ttgagactgg atattgattt tgataaaatt atagtatatt
    27961 tacttgtcga ttgttgaaaa ttttaaccca aaggttccat tatgcataaa ggatcgaaaa
    28021 gaatacttct cctatcggct atggctgcta tggccgtgct taccatttcg tgtaaatgca
    28081 atagggaatc ggagcagatg ctccaggtat ccgaagagca gggtgctgcc gatagcgctt
    28141 tggttagcag tattgaatcg gttaaacaga ttttttactc gttgccatcg ccgctggaaa
    28201 ctgcaatgat acttaagcgc tcaggggcaa catacaatga ggagttgctt aatcctattg
    28261 agaatgcatc aaaatacaat actaccaaga gcatggcgct taacctgggc atttatagca
    28321 ccgatttgag ctatgcaagc ctttttgacc aaacccaggc caccattaag tatatgactg
    28381 tttcaaagaa aatggcagag ggtttaggaa ttttaaatgc aattgacaat tcggtaattg
    28441 agaagctgga ggagaatatc aataaccgcg atgttatcct agagaccata tcggaaacat
    28501 ttctcaacac caactccatc ctggaggagg acgatagggt agctgttggt tctattattc
    28561 ttgttggtgg atggattgag ggtctttaca ttgccacctc gctggttgag gatatcaata
    28621 aggttgataa cgaaatggtc gatcgcatta tcgatcagaa gttatcgcta caaaccgttc
    28681 ttaaacttct ggagcaaagc aatagcaatg ccgatgttag ggagctgtat tccgatatgc
    28741 ttgaacttga aaaggtatac aacgatgtaa aaatcacagt atcggatgta aaagttatac
    28801 aggatggaaa atcgaaggtt gctacactga aatcgaagaa tgttacaacc gtaagccctg
    28861 aagcttttaa aaagcttaaa acaaaagtga ttgaattgcg taataaatac acagcataaa
    28921 atggttcgta tgagatcatc gatttatata cttgtttcgg cagttgttct atttcttgca
    28981 ctcccttcgt cgtccaatgc tcagtgtaag aactttgcca aaaagatatg taggcttgaa
    29041 ctttcgccct atgttcacga tggtaactac aatgcagcaa tactaaccga gggtgaagat
    29101 gctgaacttt tcaagacctt ctatgctggt cagcagtatc gcatatccgt ttgcggctct
    29161 gaaactctta ataagattga gtttatggta atggatgttg accgtaaggt gttatacaca
    29221 aatgctaaaa acaattttgc cacctcatgg gattttaagg ttgaaaatac tcaacagctc
    29281 ataatccttg ttaaggtgcc ggttacatca ggtaaaaatg aggagttgaa gagtgggtgt
    29341 gtggctatta tgtttggtct taaaactgac taatcaatta cttacataaa taataaaagc
    29401 cgctttaagc ggcttttttt tattgctata cctaattgtt aagcttaaca gagaggaact
    29461 tacctgtgta ggaattatcc atcaaggcaa gctgttctgg cgtgcccgaa aaaattaagt
    29521 gtcccccatt atcgccaccc tctgggccta aatcaataac ccagtcggcg cacttaataa
    29581 cctcaaggtt gtgttccacc agaacaatgc tatgtccctt tgcaattagg gcatcaaagg
    29641 cacttaacag ctttttaata tcgttaaaat gcaggccagt ggtaggctcg tcaaaaatga
    29701 aaaggatagg tttatcggta ttctctttga gcaggaacga tgctagctta accctttggc
    29761 tctcgccacc gctaagggtg ctcgacgatt gccctagttt gatgtagcct aaacctacat
    29821 cctgcagagg ctttagcttt tcaacgattt tcttttcaag gcttcctttg cctttctcaa
    29881 agaactctat ggcctggtta acagtcattt ccagtaagtc atacacattg gcattcctat
    29941 accttacctc aaggatgtcg tccttaaaac gtttaccgtg gcagctctcg caggttaggg
    30001 ttaggtcggc catgaactgc atctccacgt gaataacccc ttcgccctgg cactcctcgc
    30061 aacggccacc atcaatgtta aaggagaaat gcgatgggcc gtaaccgtta cttttggaat
    30121 agggctgttc tgaaagcagc ttgcggattt cgtcccacgc tttgaggtag gtaacagggt
    30181 tgctgcgcga tgatttgcct atggggtttt ggtcaaccat ttccacaccg gaaataagac
    30241 ggatatcgcc agctatgcag tcgtgctggc ctgcccgctc gccactccca gtaatttcct
    30301 tgagtagggc agggtatagt attcccttaa ccaagctcga tttgcccgaa ccgctcaccc
    30361 ctgtaaccac tgtgagggtg tttaacggaa actttacatc aatattcttc aggttattct
    30421 cgcgtgcccc ttttacctca atatagctat tccacttgcg acgcttaaac ggaacttcaa
    30481 ttttttcggt tccgttcagg tatttagcgg taagactagt ggatgcagag ttgattaggc
    30541 ttggctctcc ggcaaaaacc acctcacctc caagtaggcc tgcatgcggc ccaatatcga
    30601 taatgtaatc ggcattgcgc attatgtcct catcgtgctc taccactagc acagtatttc
    30661 ccagatcacg cagattttta agaacatcga taagtagatg ggtgtcgcgg ggatgcagac
    30721 caatactagg ctcatcaagt atatagagcg accccactaa actgctgcca agcgatgtgg
    30781 caaggttaat gcgttgcgac tcacccccgg aaagggtagc ggacaaacgg ttaagggtta
    30841 aataacctaa cccaacatta accatgtatc ctaaacggtt tcgaatctct gttaacaaac
    30901 gttttgccac tgtgacatcg tgctcatcca actcgatgtt cataaagaac tcgtaaagct
    30961 cttcaatctg gatgttaact aactcctgaa tggtgtaacc agccaccttt acatacgatg
    31021 cttcaggtct aagacgtcca cccttacaaa ccgggcatgt ggttttgccc atgtagcgtg
    31081 agagtaaaac tcggtattgt attttgtatc gtttttcctc aagctccgcg aagaaatcgt
    31141 aaataccctt gaagtgcctt gtgccattcc aaagcaatgc tttctgctcc tcagttaatt
    31201 tgtggtatgg ggtgtggata ggtaggttat acgccgatgc atactcaatt actctgtcct
    31261 tataccaacc cattgactcg cctcgccagc acgcaatagc atcctcgtaa atggaaaggc
    31321 ttttgttggg gactaccaaa tcctcgtcta tgccaattac cttgccgtaa ccctcgcatt
    31381 ttgggcaagc accaagtggg ttgttgaagt tgaaaagatg ctcggtgggc tgttcgaatt
    31441 ttatcccatc gagttcatat aggttcgaga attgctcctc ccgtattcct tcgtcggttt
    31501 cgatagaaag aatgcaaaaa ccatttcctt ctgcgtaagc ggtttgaacc gaatcggcta
    31561 tccggctaat aaaatcttca tctaccgaaa cggcaaatct atctattatc aaccttgtgt
    31621 tggcagcagc caaatcgaca agttcacggt tttgagcatc atcaatccta atcactttac
    31681 cgttaatatc aattcgggta aacccttcgc ggtttagttc ctcaagcttc agcttgatat
    31741 tgccgttaac ggcaataggt gctaaaaggg tcatgcgtgt tccctgctga tgggcactga
    31801 taaagtttac aacatcggta accgagtgac gcttaacctc gtggcccgaa atgggagagt
    31861 atgtttttcc gatacgggcg aaaagtaact taaggtaatc gtaaatctca gtggatgtgc
    31921 ctacggtaga gcggggattt cgggtgttaa ctttttgctc aatggcaatg gccggcggaa
    31981 taccgctaat aaggtcaacc tcaggcttgc tcatcctgcc aaggaattga cgggcgtaag
    32041 ccgataggct ctctacgtat cgcctttgcc cttcggcata aatggtatcg aaagctaacg
    32101 atgattttcc cgaacccgat acacccgtaa ttactaccag tttgttacgg ggaatatcta
    32161 cattgatatt cttaagatta tgaacccttg cgccttttac cgatattacc ttttcgtgct
    32221 tttgcattga tgaaatggaa aaatgtaacc tacaaaggta ttacttcaaa accatttttt
    32281 aatgatgctt tttcaaattc tctgaaatta tactttcctt cccaagcccc cttgttttga
    32341 aaatttctac taaccctttc atcataactt ttaaaaatgg tgtaactaaa tggaaatcta
    32401 atttgtgtct ttttatcctt taaccttaaa actttaactt caaaccttca acctcgaacc
    32461 atgaaccatg aactttgaac ctttatcctt tatcctttat ccttaatata tctcttgcca
    32521 ttagcccctc gttaaaaagc aagtcaataa cgctgaggtt gggagtaaac ccaaagcgat
    32581 gttcaaaaac ctgaaagtat ggtagggtag cgtgcaagct ggtttcaggc attttagcct
    32641 ttgggctaat tgcgtaacgt aaatccaata ctccttcggg ttcaacttta tgaaaggtac
    32701 ttgtaaaatt gatattaacg ggtagctcaa ggaagtccaa aattgccttt aggagttcat
    32761 tgttgaactc ccacagggta ttgaaagggt tgctgtaaaa gggctttagt acatcggcaa
    32821 taaattcgaa atatgccgaa gttcggtaag ccgattcgat ggcacgccaa tgattcctgt
    32881 tccaaccggt agagtaatca ataataactt cggttatacc ggtatggttg ccctttggtt
    32941 ttattactgg gattacaagg ggtaatgggc cgtttgcgct gagtattacg catcggttac
    33001 ggtaggtttg tttaaggtaa gtttcatgtg cctcaataat aattttatcg gcactaagta
    33061 aggcggacat gtaatgaacc ggcggtaaat acgctgtgct aagtagtatg gttttactgg
    33121 acattgtatg ctttcattga actaaaaaac ctgttgcggg ggccattccc ctccaacagg
    33181 tttttatgca gccattgtta atcctattta atggttgtaa acatacgctt aaagcgtata
    33241 ttggcaggaa aacgtttatc cttatcgagc gatagccaaa taaacgaagc ttttcctacc
    33301 acatggtctt cgggtacaaa tccccaaaaa cggctgtcgg ccgagttatg tcggttatca
    33361 cccatcatga aatagtaatc catcttaaat gtataagagc ttgctggttc accattaata
    33421 taaatagtgc cattttcaac cctcaggtcg ttgttctcgt aaacatcaat aatgcgttgg
    33481 taaagtggca ggttatcggt ggtgagggta acggttgcac cttttttggg tatccatagg
    33541 ggcccaaagt tatcctcgtt ccatttatag cggggatcgt gggggaaaat taacttccac
    33601 ataactgtgg agtcagtatt ctcgtttcgg gttatttcag ttacattgct aaaggtttta
    33661 aaacgttcca gcatttcctc ggttaacgga aggatgtaaa ccgattcctg cgcattgtag
    33721 ttgatatcgg aacgggcaat acccatttcc tcaaaccgta ttgggttaat ggtagagcca
    33781 ttagttctta tctggtagtt atactgtctt ttaccaatct ttgcctgctc tttgccatta
    33841 acaaaaaggc taccatggac aacctgaatg gtgtcgccgg gaatgccaac gcaacgcttg
    33901 atatagttct cgcgtttatc aacagggcgg taaataatat cataattttg acggatgtag
    33961 tcgcgtccat aaattcttgc cagggtgtag taacttgaat tctgatcctg caggcagacc
    34021 gtatcgcctt cggggaagtt gaatacaacc acatcgttgc gttttatctg accaaaacca
    34081 gcaatacgtt tataaggcca acggggccac tcaatgaacg acttggtgta ttttgtaaaa
    34141 ggcagggtgt gatgaacaaa tgggaatgaa attggcgtgt tgggtagttt aggcccgtag
    34201 gcaaccttac tcacaaatag gtagtcgccc accagcaacg atttctccat cgatgatgtt
    34261 ggtatggtgt atgcctcaat aaaaaacatt cggattaggg ttgccgcaac aactgcaaat
    34321 attatggcat caacccattc aatcagaacg gtcttctttt gcccgcgctt cttccagaat
    34381 gtccagttta ctttttgggt gatgtagaag tcgaaaatga taggtaatcc cagaagtagc
    34441 cagtagttgc caatccatac tacccagagt atgaaaatta tggctgcaaa tgtaaatcgg
    34501 aaatacttgt tttttaggaa atcttttact ttttgaaaca tggtattgtg gtttagtcaa
    34561 gtttaagcaa atcatccatg ctgtaaaatc ccttgcggga gctgagaaac tcagcggcta
    34621 ataccgctcc aagtgcaaac cctctgcgac tcttagcctc gtgctttaga gtgatgatat
    34681 cctgttcgga tgtaaaggtc acaatgtgag taccgggaac ttgaccctct cgaattgcct
    34741 caattgggat ttttctctct gcactatcgg ggattaagct ccagccttcg tatgccttca
    34801 tttggttgca cagtgtattg gcaatggtta tggcagttcc gcttggagca tcaagctttt
    34861 gggtgtggtg tatttcggtt attgatggtt gatacatccc tatcctgtca agtatggaag
    34921 caatgtaatt attcagcttg aaaaagatgt taacaccaat gctatagttt gatgcgtaaa
    34981 acaacgttcc cacgccattg cgtgccttgt taaccatgat ttcaagttgg ttgttccagc
    35041 cggttgttcc tgataccaca gggattccag cgtcgaaaca ggcttgaatg ttttgcatgg
    35101 ctgtgctcgg cgaggtgaat tcaatggcaa catcggattt ttttaggttt tcagtgttaa
    35161 ggtcattgat gttatccttg tcaattctca ggacaatagt atgtccacgc tcaagggcaa
    35221 tagcctcaac ctcatgcccc atttttccgt agccaattaa tgcaattctt agcattttgt
    35281 taaacacctt atttttgcct gcaatttatt aaaagatgac gagaaatgat ataaacagca
    35341 gcaagttaat caaaattaac aaaaaagggg catttagccc ctttaacctt attctgtttt
    35401 actggttact ttacagaatc aacaatagct ttaaaagcct cagggtggtt catggccaaa
    35461 tcagccaaaa ccttgcggtt aatagcaatc cctttctggt tgattttgcc catgaattcg
    35521 gagtaggtca taccatactc acgaactgca gcgttaatac gctgaatcca tagggcgcgg
    35581 aattcaatct ttttcttctt acggtcgcgg taagcatagg tcaaaccttt ttccaaggtg
    35641 ttctttgcaa ccgtccaaac attacgacgc gcaaggaaat ttcccttcgt ctgtttaaga
    35701 attttttttc tgcgcttgcg cgatgctact gcatttactg aacgtggcat ttttttacga
    35761 tttttgaatg attagcggtt caacgttttt gaactcttag tggctaattc attctgttaa
    35821 acattaaagt aaactaacac acaaactaaa agaaaacaaa actacttcaa gccaagcatt
    35881 gccttaacat gcccttcatc ggccttatca accaaagcaa cgtgagttaa attcctcttt
    35941 tgcttggttt cctttttagt caaaatgtga ctcttgtagg cgtgttttct tttaatctta
    36001 cctgttccgg ttaaagcaaa acgctttttt gaaccagaat gagtctttgt ttttggcatt
    36061 ctacactgta tttatttgat tactaatggt ttgttatttc ttctttgtgc cttttgggga
    36121 tactatcata atcattcgtt tcccctcaag ctggggcaac tgctctactt tgccatactc
    36181 ctcaaggtct tgggcaaaac gcaaaagtag tatttcgcct tgctccttga aaagaatgga
    36241 acgtccctta aagaacacgt aggctttcac cttagcacct tcctgaagga aattgatggc
    36301 atgcttcaac ttgaagttgt aatcgtgatc atcggtattg ggcccaaacc taatttcctt
    36361 aaccacaatt ttttgagcgt tagccttaat ttccttttgc ttcttcttga gctggtaaag
    36421 gaacttctga tagtccagta ttttacaaac cgggggatcg gccttatcag aaatcattac
    36481 caagtctaac tccatctcct cggccatttg acgtgcctgg ctaatggggt aaatgcccgg
    36541 attctcaatg ttttcgccaa ccagtcgaac ggtcttagcc cgaatttctt cgtttatcgg
    36601 gtaagtgtcg atatccgact ttttgttgat attaggtgct gctatagttt tgccctccta
    36661 acgtttagtt aatactaaat ttccttaagc tgattatcaa tttcagcctt tatatggttt
    36721 acaaactctt caagtttcat ttggccttta tcgccctcgc cttgaacacg aacagcaaca
    36781 aggttggctt cctgttcctt ttcgccaaca attaaaaggt aagggattcg gcgtaactcg
    36841 ttgtccctta tctttttacc tatcttctcg ttgcggtcgt caaccaggct gcgaatatcg
    36901 taattattta ggaattttga aacacttttt gcataatcgt taaatttttc gctaatgggt
    36961 agcactacaa cctggtcggg tgttagccat agcgggaatt taccgcctgt atgctcaata
    37021 agtacggcaa caaagcgttc catcgaccca aagggtgcac ggtgtatcat aatggggcgg
    37081 taacgcttat cgtcagcacc aatgtactcc agctggaagc gctcgggtag gttgtagtca
    37141 acctgaatag tgcccaactg ccacttgcga ccaatagcat cgcgaaccat gaagtcgagt
    37201 tttgggccat agaatgcagc ctcaccaagt tcggttacgg tttttaggcc tcgctctgct
    37261 gccgcctcaa ctatagcctt ttcagccttt tcccaattct cgtcagagcc tatgtatttc
    37321 tctttattgt tcgggtcgcg tagcgatatc tgtgcggtgt aatctttaaa atcgagagtt
    37381 ttaaagatgt atagaacaat atcgataacc tttacaaact cttccttgag ctggtcgggc
    37441 ctacagaaaa tatgtgcatc gtcctgggta aatccacgaa cgcgggttag accgtgcaat
    37501 tcaccgctct gttcgtaacg gtaaacagtt ccgaactcag ccaacctgag cgggagatct
    37561 ttgtatgaac gcggtttgga tttgtaaatt tcacagtgat gggggcagtt catgggttta
    37621 agaaggaatt cctcgccttc ctgtggagta gttatgggac ggaagctatc ctttccatat
    37681 tttgcccagt ggcccgatgt ttcgtaaagc tctttttggc caatgtgcgg ggtaattacc
    37741 tgctcatacc cgtatttctt ttgaacacgt ttaaggaaca tctctaagcg ctcgcgaagt
    37801 tgagcaccct tgggtaacca taatggtaaa ccagggccaa cacttggcga gaacataaat
    37861 aactctaaat ccttaccaat tttacggtgg tcgcgttttt ttgcttcctc aagcagggtc
    37921 aggtactcgt cgagctgttt cttttggggg aatgtgatac cgtaaatgcg ggtaagcatt
    37981 ttattttttt catcgccacg ccagtatgcg cctgcaatgc tgagcaactt tatggcttta
    38041 atgtagcccg tattcggaag gtgtggtcca cggcaaagat cagtaaattc gccctgggtg
    38101 tagaacgata tggttccatc ctgtagatcc gatataagtt ccaccttgta aggatcgccc
    38161 ttttcctggt aggtcttaag cgcttcggct tttgaaacct ccctgcgaac gatgggttgc
    38221 ttttgctggg caaactccat cattttttgc tcaatcttag gtaaatcctc ttccgatatg
    38281 gtgtgtccgt taaaatcgat atcgtagtaa aacccattct caatgcttgg gcctattccc
    38341 aacttaacgc cggggtaaag cgcttcaacg gcctcggcca taaggtgtgc cgatgagtgc
    38401 caaaaggcat gttttccctc tgggtcgtcc cacttgtgta gcttaacggt ggcatcagtt
    38461 tcaatgccac gggtaaggtc ccacacttca ccattaaccg ataccgataa aacatcggct
    38521 gctaaacgct ccgaaatgct tttggctatt tcaagtccgg taacaccttt ggggtattcc
    38581 cgcacgttcc catcaggaaa agttatttta atcataacag tagaatatca attcaaaatg
    38641 acttgcaaaa ttataaaaat atttcatacc acattcatgt gtggttgggc ttgtttaata
    38701 aaacaacaat tgttgagttg ttgttgttta gttaaggtta tttgtcatgt tatgcgcaag
    38761 cgaaacatca cgctgccgtg ctgagtcccg atttaaaggg atagacttcg ccgaagcacc
    38821 tcactactca ttttcatcaa tcctatgatg agactcctcg acattgcttg ggatgacaaa
    38881 aaccctatct tcctttctgc tcccatgtca tgctgagcta gccaaagcat ctctttgcct
    38941 tcaatccttg ttgttttttt tggaaatcgc tcttagcaat gctcttcagt tgcgatgcaa
    39001 tttttccact ttgctctttg gaaaatgtat tgcgtgcatc gcaatacaag tgagagcaaa
    39061 catagggtta caaacgattc gcccctacgg ggctggaagc gttgttagtt gttcgtggaa
    39121 gacgacatca cctcatccct tttccctgag agggaatgga gcggaagaag caagcagagg
    39181 cataaaagcc agacccggca atgggtcggg ggtggaacgt ggcggtgggc ttagtaagcg
    39241 ataccacatg agtcacgcca taggcgtggc gaatagtatc gcgtggcagt tccacccgcg
    39301 gggtacccat cgggtcgggc ttttcagcct tgattttctt tggttctttc ttgtatcaag
    39361 acaagaaaga acgtgaaacc gctctttgta atgcttttcc tcagcgatgc aatgcctcac
    39421 ccttgctttt tggcatacgc actgcgagca tcgcaataca agtgggagca aacataggga
    39481 tacaaacgtt tcgcccattc ggggctggaa gcgttgttcg ttgttcgtga attgttgttc
    39541 gtgaatctat tatcatattg ggctaagcga aacgtctcat ggggtcgatt agagaacctt
    39601 cgctgcgctc aggatgactg acatgtatat ttttgttaac taaacgacat tgattgttga
    39661 gttattgttt ggggttaacc tgattggttt tacccttttt ctttaatgaa aactttgatt
    39721 cttcgccttt taccagacgc tcgatattct tttgatgggt gaatagcatt aggattgcca
    39781 gtataaaggc aaaagtaatt agcgatgggt tagtagtgcg gtaaacaaaa atcagaacca
    39841 gtgggaacga aaatcctgca atcatggaac ttaacgaaac gtatcgggtg attagcaccg
    39901 atactatcca aattccaaat actatcaaag tggcgtaggg gtgaagggca aaaacaacac
    39961 ccgatagcac tgccacaccc tttcccccac gaaactgagc aaaaatgggg aatatatggc
    40021 caagcaatgc tgcaattccc aaaaacagct gaaagttgat aaacattcct gttttaggga
    40081 tatagaaatc ggttaggtat agcagacgaa ctgcaaggta acctttaagc acatcgacta
    40141 taaagaccga tattccagga atcagaccaa gcaccctgat ggtgtttgtt gcaccggcat
    40201 taccactgcc atgctcccta acatcgattc cgtaaaaaat ttttcctacc caaaccgatg
    40261 taggtataga gcctaaaaga tatgccgcaa atattacggc aattttgagc atagtttcca
    40321 tgctgcaaag atacaaactt tgatggcgaa agaatatcta tttatatcta tttttaaatt
    40381 tcaaattctg taaatgattg attagtagtt ggtaataata tatgctttag gttacgatta
    40441 atatttttta accgcaatgc aggttgattt aaatattttg ggtagggaca tttccctact
    40501 caacaaggct aataagctgc tccttgttaa ggagtatcat cggattattg gagttgacaa
    40561 attcgtgggc aaggttttcc ttgtgggctt gcagcttaac aattttctcc tccaccgtgt
    40621 ttcgtgtaat aaaacggtaa acaaacacac tcttggtttg cccaattcga tgtgcgcgcg
    40681 atgctgcctg catctcggcg gcaggattcc accatgggtc aagaataaaa atgtaatcgg
    40741 cagcggttag gtttagtcca acaccgccgg ctttaagcga aatcaaaaag attttctttt
    40801 caggattgtt ctggaactct tccactacat cgcttctgtc ggttgttgaa ccggttagca
    40861 tttcgtaacc aataccgttg ttttgtaagt aggtttcaac ctgtacaagg tgtttaacaa
    40921 acgatgagaa tatgagtatc ttatggtttt ccgcaataac actttcaatc atttgcgtaa
    40981 cttcctcgaa tttacccgac gacaggtttg agtattcatc gagcatggcc gggtggttgg
    41041 ccagctggcg caaacgggta agcgctgaaa gaacattgat ggttgacgat tcaggtttac
    41101 tctgttccag ggagtcaagt atcaacctgc gggcttttga tttctccgcc tcataggctt
    41161 tgccttgctc gtcggtcatc tcacagtaga tgatttgctc ggttaggggt ggtaactcct
    41221 tggcaacctg ctccttggtg cggcgcatta tcaggggttc aatgagcttt tttagctgtt
    41281 tctgcttttc ggcgtcattc ctgcgctcaa tgggaatggc aaattcctca cggaatacct
    41341 taaggccgcc gagcatcccg cggtttagga agttcatttg cgaccatagg tcaaataggt
    41401 tattctcaat gggtgttccg ctaaggcaca agctcttctt tgtcttaagc aatagaacgc
    41461 acctgtatat tttagatgct gggtttttaa tggtttggct ctcgtccaac accacgtagc
    41521 ggaatgcaaa gtccttgaaa aggtcaatgt cgttccgtat ggtgccatac gatgtaatca
    41581 caacatggtt ttgctgaaat attttgtggc tcttgtcgcg ttgggttcca tggtaaatcc
    41641 caactttaac ctgtggcgca aacttttcga actccttttg ccagttgtaa attattgaag
    41701 tgggagcaac aaccagcgaa cagtgttcct gtccggtttt aaaatcgtta gcctcaagtt
    41761 ccgagagtaa aagtgtaatg gtttgtagtg tttttccaag gcccatgtcg tcggcaagac
    41821 agccattcaa gccgttttca accaatgttt ttagccaggc aaaaccaagg gtttggtact
    41881 cgcgtagttt tgcctgaagg ctatggggta gggttacccc tttggcgtta atatttttta
    41941 gtttttcagc gaaacttgaa atactctggg tttctaggct actgcctgca ataattgctg
    42001 ccgatgcttt ggacagctta atgctttcgc cttcctcctt gccaagcata aggatggttc
    42061 tgtacttggc aaaccactca tcgggcaaaa tgaaaacctg accgttgggg agggtgaact
    42121 cgcgaatgtt cttaactata tgcttgcgga agtaaacaaa ggggatttca aattcgccaa
    42181 tgcgaaccat gccgtaaagg tcgaaccaat cgatactttc ggtaagcctc aaattaaggt
    42241 caaagccact taggtagtag tttgtttgcc taaacttttg atgaatctca aaaccttcgt
    42301 cctgtaaaag cttatagttg gtgttcagcc attctatcac ctggtagtgg tcaaactcgc
    42361 cattttcggg tttgccggga aagtactcgt tttctcccac ctttgttagg cgccaggttt
    42421 taataagctg ttgtttgatt ttttcttcct tctcggggtt gcgggtaagc cgggtaaatg
    42481 tgtatgagtt attacggaac gatagcttta cttcattaac agcaggcgtg tttgccgaaa
    42541 taaccttatt cccatactga aagctaaggg ttaggcaggc tccgcctgat aggcgctcat
    42601 cgagcgtgag tatgggttta ggcctgtttt tttgttcgtt gatttcaaat cccacagctt
    42661 ccacctggtt gcttctaaca gcattcagca caaaggtttc catatacttt tgtaccgatt
    42721 gttggggtat tgtaacaaat tgcttggtca tgtagggtac aaactttcgg ccatcaatac
    42781 cttcaacctt atatatgcca ttggcaaccc gaattaagca tggttcaacc gtaagtaatt
    42841 caaacacacc gggcaataaa gttaaaatgg aagtttgatg ttgaatcttc agggaatact
    42901 tcatgccaag ctcatcaagc tcgaacctga atatgggcga cgcaacatcg gtatggtatg
    42961 ttaattgctt ttccttgtaa atgttattgg cgttccgctc tctaatatat atcgggatat
    43021 tggccgatgc ggctttttca acggccttac atattcttcg gtcaatgtag ttcctgatgt
    43081 agctgtttag gttttcgtcg ctttgaactt tttgtaaaag ttcgttgaaa gttttcgcat
    43141 cgcgtccaaa tagcttcaaa aggctttttt ctgatatttc ctgaatgctg cgtattattt
    43201 ccgattggtc ggttgtaatc ctgtcggaat gaataactgc tgaatcgaca ataacattct
    43261 tttcaatttt ataggtatca tcgctgattg gactgataag ataagctcct gccaccaaac
    43321 ccaattccct gtgcctatgc aaagcaatcg caaagatgta ttgtgtatca gttgaatcca
    43381 tttaggctat tttactgcaa ttttaacaaa aatatgcgta gcttaatgtt ttttatgtat
    43441 caatttttac tttcttcgtt ttaaaacagc attaggcata gggtttgaat tgattgattt
    43501 gtgtattttt gtactttgta ttcgggccca tttcggaata acattttcat aaaactactt
    43561 gcaatgatta aaagaatact tgcactccta actctggtat cgctttccta cattttgttt
    43621 tcgcaggaag tagaggtaaa aatatcaaag gataaggtac gtgtcgatgg gaaaacgtac
    43681 tttgttcata tagtccagca gggtgaaacg ctatactcta tcagtaaggc ttacggcgta
    43741 agccagtccg acattgcgat aaacaacccc gatatatacg cagggttaaa ggtgggacag
    43801 gctctaaaaa taccggctaa agtgaaggaa acccaaactg acgaggatta tatctaccac
    43861 attgttaaac gcaaggaaac cctttttggc atttcaagga tgtacaatgt gagtatggat
    43921 gatatactac ggttgaaccc tgaagcaagg gaagggttaa agctaagtca aacccttcgc
    43981 attcccaaaa aacagataag cgatattgag cagcaacccg ccgttgattc agtagaattt
    44041 attttgcacg aggtgcaacc ccgcgaaggc ttattcgcaa tttcccggaa atacggtgtt
    44101 tcacccaagg agattgagta ttataactac gatttaatta aggatggttt aaaacttgga
    44161 acaacccttc ggataccaaa acccaaatcg gtacctgttc ctgaggctgt tgaagccaag
    44221 gttgataccg atgggcagga gaaagcatcg ccctgcagta atagctacac ctacgatggt
    44281 catccgttta atgtttctct actacttccc tttactcaat ccggcagcca gactcaggcc
    44341 atagttgatg atgttgaccc aaaagatgtt caggacccca cagggcaatt aactcaaata
    44401 acacaggttt cgcttgaatt ctacgaaggt ttcctgttag ccctagatac tcttaaaaag
    44461 ttgggcatct cggtaaacct aaacgtgcgc gacacaaagc gctcaccaca ggaagttgct
    44521 aaaattctga atagcgctga ttttgcaaga tctgaattag tggttgggcc ttatatgttc
    44581 gatgaggtta agcctgtagc tcaatttgcc gccaaaaata atatcaactt tgtatcgccc
    44641 atttacagca ttaaagaggg gttggctgat aagcagaata ttatttcggt tagccaaaca
    44701 cttcaaaacc agttggaaat atttgtgaag aacttgaagg ttgatccaag taagaactac
    44761 ctggtgattt atgactcaac catctggtac tcgcccagtt tgaagcattt cgattcgctt
    44821 ctaaccctaa gcttcacaaa cgcagctatt cctatttctc ggtacaacca taaaactgct
    44881 gcttacaaat cggttgatgt tcaggaaagg ttgctgaaga tgctaaaacg cgatgccgta
    44941 aacgttgtga ttgtgccttc cgacgatgaa ccgtttgtag ccgaaatact tggcagttta
    45001 tatgctgtaa aatcgtacta taacctcaac actgaggtgt atggcccatc gcgttggcgt
    45061 aaacttaaaa accttccccc cgattacctt tttgcattga atactcatgt attctcacca
    45121 ttttttgtcg actactctca aagtgatgta aaaacttttg tagagaaata cagggtacaa
    45181 ttccggtcgg agccaacaca gttctcgttt ttaggttacg atgtggcctt ttactttatt
    45241 aatgcactca aggagtttgg tcctggtttt actccctgtc tgccaaattt caggttaaga
    45301 acgcttcaaa ctaatttcaa gctcaatacg agtaaagagg ggtatacact taattcaaat
    45361 cagtttatag taaggtatgc ccccgatttt acaatcacat gcagctgcta ttaatggatt
    45421 taaattaaaa ttttatcatg aattatcggt ttaattccgg aaatacccgc ctggttaatt
    45481 ttgagggttg gctctctgtc ttaatcaaca ctttgctttt tgtactaaag tactgggcag
    45541 gggtggttac aggatcgtta gcgcttattg ccgatgcctg gcataccctt tccgattcaa
    45601 tcagctcagt tgttttgctg cttggcataa aattttcgca aaagcctgcc gataaggatc
    45661 atccttttgg acatggacgt tcggagctag tggtttcttt gcttattggt gcactgcttg
    45721 cctttgtggc tttttacttt gccatggaag gtattgcaaa aatcagggat agggtgagta
    45781 ctagctatgg gactctggca attgtagcaa ctgtggtatc aatagttgta aaggaaggac
    45841 ttgctcaggt agctttcatc ataggtaaac gcacaggctc aatttcagtt acagccgatg
    45901 gttggcacca ccgcagtgat gccctttcat cggtcattgt gcttgtgggt atttttgtag
    45961 gcaagtactt ttggtggatc gacggggttt tgagtatcat tgttgctctg ctcattcttt
    46021 actcaggtat taaggtgatt accgatagtg cagtggtaat tcttggcgag gtaccatccg
    46081 atgatcttgt tcaaaaagta atggagattg tctcaaggca gggatatgat agaatggagc
    46141 cccatcactt ccatatccat aactatgtaa tgcatcaaga acttaccttt catgttaggc
    46201 tgcccaatca aatgaccata gaacaggccc atagaattat ttcattaatt gaagatgcca
    46261 tacacttgga gcttggcatt gatgccacta tacatataga accccaaact accgataagc
    46321 attgcaagca tatcgattaa tctttttggg gatttaatgg gtgtacgatt atctacatta
    46381 ttccccttaa aagtaacaat atgatacaaa acatgctgaa aaaatcgttt gcataaacta
    46441 aaatatttac atttgcttat aattctcaat tgtttattct taaaaaattg tggaaatgat
    46501 tgacttatcg cagtacggta ttaattccct tagggagtta tactataatc catcgtacga
    46561 tttactttat gagcacgaaa ccgacccaaa tcttgagggt tatgagcgag gttttgtaac
    46621 caaacttgga gcagtaaatg ttgacactgg tgtatttaca ggccgttcac ccaaggataa
    46681 gtatgtggtt cgagagccaa ataccgaaaa tgacgtttgg tgggccgagt ctggcaaaaa
    46741 gggttccgat aataagccca tatcgcaaga ggtatgggaa aagctcaagg aggtaagcgt
    46801 taagcagctt aatggcaaaa agctatacgt ggttgatgct tacgctgggg ctaatgagga
    46861 tacccgattg agcgttcgct ttatcatgga ggttgcctgg caggcacatt ttgttaaaaa
    46921 tatgtttatt cggccttccg atgaggaact caaaaacttt acccccgact ttgttgtgct
    46981 tgttgcatcc aaaaccacca atccatactg gaaggaacaa aacctgaaca gcgaggtata
    47041 tgtggctttt catcttactg agcgaatggc cctgattggc ggcacatggt atggtggaga
    47101 gatgaaaaag ggtatctttt ccgtaatgaa cttttaccta ccccttaagg gtattgcatc
    47161 gatgcattgc agcgccaatg ttggcaaggc tggcgatgtg gcaattttct ttgggctgtc
    47221 aggcacaggc aaaactaccc tttcggctga tcctaatcga gcccttatag gcgacgatga
    47281 gcatggctgg gacgattcgg gtgtatttaa ctttgaggga ggttgttatg ccaagtgcat
    47341 cgaccttgac ccacaaaaag aacccgatat ctttgcagcc atccggcgcg atgcacttct
    47401 tgagaacctc gatgtggatg ccgaaggcaa tatcgatttc cattcaaaac gtaaaactga
    47461 gaatacgcga gtatcatacc caatttacca tattaagaat attgttaaac ccatttcgcg
    47521 tgctggacat gcaaagaatg taatattcct aaccgccgat gcgtttggtg ttttaccccc
    47581 ggtttcaaag ctaactccta accaaaccca gtaccacttt ttaagtggct ttactgcaaa
    47641 gctggcagga actgagaggg gagtgcttga gccacagcca acctttagta gctgttttgg
    47701 gcaggccttt ttgatgttac accctacggt ttatgctcgg gagcttgtta aaaaaatgca
    47761 gcaacacaac tctaaggctt accttgtaaa caccggctgg attggtgggg cttatggtgt
    47821 tgggcgtaga attgatttgc cctcaacccg tgccattatc aacgcaattc tggatggtag
    47881 cctcgataac gctgagttcg atgttcttcc aatttttaac cttcaaatac ctaaggcatt
    47941 gcctggtgtc gattccaagc ttttaaaccc acgtaaccac tggtcgaatc ctgaggattg
    48001 ggatgcagct gcccggaatc ttgcccaaaa atttatcgaa aactttaaga cctttactga
    48061 taacgagtgg gggcaacgat tggttgctgc tgggcctatg ctgtaactgt tctccgaaaa
    48121 aatcaaaacc taaaattact atacaagaat ggcaaccatt ttggtagcca ttcttattct
    48181 tgttcaatac tacatgtttt gtaagaaagc cttaacgttt ttctcaatcc tattcagaat
    48241 atcctcgtcg ctgtgagcct tttggaatct gtcgccagta attttctcgt acagctcaat
    48301 gtagcggttt gatacctgct caataaattc aggggtcatt tcgggtacgg tttgcccctc
    48361 cttaccttgg aaaccattgg ccatgagcca ttcacgaaca aattccttcg agagttgacg
    48421 ttgctcctcg cccttctcaa accgttcctt gtagccatca aggtaaaagt accgcgatga
    48481 gtcaggcgta tggatttcat caatcaggta tattttccca tcctttttgc caaactcata
    48541 cttggtgtca accaaaataa gacccatttt ctcagcaatc tgggttcccc gttcgaacaa
    48601 tgccagggta tatttttcta gaaggttgta atcggcttcg ctcactaaac ctttcgcaat
    48661 tatctcttcg cgtgaaatgt cctcatcgtg cccctcttcg gctttggtgg tgggagtgat
    48721 aataggtttc gggaatttct gatgctcctt catgccttca ggtaggggaa caccgcaaag
    48781 tgagcgcttg ccggatctgt actcacgcca agcatgacct gtcaggtagc cgcgcaccac
    48841 catttcaacc ttgaagggtt cgcagaagcg tccaactgtt accattggat cgggcgaggc
    48901 aattttccag ttaggaacaa tatcggcagt tgcgtcaaga aacttgttgg caatctggtt
    48961 aagaacttga cccttgtaag gtatcccttt gggaaggatt acatcaaagg ccgatatcct
    49021 gtcagttact accataacaa gaaactggtt gttgatgttg tacacatcgc gtactttacc
    49081 atggtacact gaaacctgtc ctggaaactt gaaatcggtg cgggtgattg ctttcatctt
    49141 ttttgttttt atttggtttg attatcatca cttttcgcat cggtttgaat atcggttttg
    49201 cccttggcct cctcaaatgc gtttactatg tattttacca gcttatggcg aataatgtcg
    49261 cgggtatcaa agtatattat tgctatatct tttattccct ccaggagttt gattgcttta
    49321 accaagcctg agtcatcctt gtttggtagg tcaatttgtg taatatcgcc cgttacaatg
    49381 aactttgcat tacttcccat acgggtaaga aacattttta gctggctaag agtggtgttt
    49441 tgagcttcgt caagtattac aaaggcattg tcgagtgtac ggccgcgcat gtaggcaagt
    49501 ggggcaatct gaacagtacc atcttccata aactcctgga gtttctttgg gggaatcata
    49561 tcgtgtaggg catcgtaaag gggttgcagg tatgggtcaa gcttttcttt catatcgcca
    49621 ggtaaaaagc ccaagcgctc cccggcttca acggctgggc gggtcaatat tatgcgtttt
    49681 acctccttgt tgcgtagcgc ccttactgca agggcaatgg ctgtatatgt tttccccgat
    49741 ccagccggac caatggcaaa aatcaggtcg ttactttcat acatctccac aagcttgcgc
    49801 tgatttaccg ttcgagcctt aacaatttta ccatggttgc cgtaaaccag cacatcggtt
    49861 atgtcctctt ccgccttttc aatggattca gggttgtttt gcaggatatc tttgatattg
    49921 gtttcactta agtacccata cttgatgtga aagtcaatca tggcatgaac tttctgatcc
    49981 aagtcctcta ttagctgctc atcgccaaca gccttaatag tgttccctct tccgatgatt
    50041 tttagctttg ggtagtgctt ggttattaag tcgaagcggg tattgttaac cccatataac
    50101 accaaggggt caatcccttc aataatgata gttctctcgc ctgctgccat caatacctgt
    50161 tgggttatgt atgcaaagat aatctaaaac cagccaactt ttgtgtttat agtcgcaaga
    50221 tatcaaaaaa tactaacagc taaatggtta gtaagtttag caggctaaat gcagaaatta
    50281 ttaattttct agtttacttt tgtattgatt tttggtttct taaatggcag caaccaatac
    50341 ccgttttgta actcttacca ccgattgggg catccaggat tactaccttg ggatgctctg
    50401 cggaaagctt tattccattt cacccaactt gaaggtggtg gagttaagcc ataaagtacc
    50461 tacttataat tacatgcatg cggcatttct gataaagcat tcctttagcc atttccctaa
    50521 agggtcaatt catatttgca tggtgaatag cgatacctct gacaacatgc gcatgctttt
    50581 ttttgaatac cagggccatt actttattac acctgataac ggaaccattt cgttaattgc
    50641 tgatgttttg cccgaagtgg ttcgtgcaat tcctgttgat gacacccgta gttttggttc
    50701 aatcgattcg gtaaccctgg ctttggaggc gctactttcc gaaaatggcc aaaccgattt
    50761 cgccattgaa gcatcgggta ttaagcggtt tacgccaatc ctaccagtcc ctgaaaacga
    50821 tgggattact ggaagcgtta tatatattga ttcctacttt aatgcaataa ccaacatcac
    50881 tcgcgacgat ttcgaagcaa ttcggaagtc ccgaaagtac tccatatatg ttcatcggtt
    50941 ctcgcatgga ataagcaagt taagtaattc atacaaggat gttgaaaagg gcgatttgct
    51001 tgcgcttttc aactccttgg gtttgcttga gatagctgtc cgtgaagggt ttgccgctca
    51061 aatgtacaac ctctcagttg gctcaaatat aatggttcgt ttttacgacg ataaaatttt
    51121 aaggtgatga atgatagcgt tagaaaacaa cgtgcaagcg aagcttttca aagattaatc
    51181 ggaatcatgg acgacttgcg cgccaaatgt ccctgggata gggaacaaac atttgaatca
    51241 atccgcccat taaccattga ggaaacctat gaactttccg agggaattct ggaaaaggat
    51301 tacaatctaa tccgcaagga acttggcgat ttgctgctgc atattgtttt ctactcgaaa
    51361 attgcagagg aggataaccg gtttgatatt gttgatgtta taaactcgct gtgcgacaag
    51421 cttatatatc gtcatcctca tatttacggc aatgttaagg ttaacgatgc gagtgaggtt
    51481 gttgagaact gggagcagct caaaattaag gagaaggatg gcaataaaac tgttctttca
    51541 ggggttccaa cggctttacc tgcactcata aaggccaatc gaattcagga aaaagtacgg
    51601 gccgttgggt tcgattggga tgagcgttcc caggtctggg ataaggttca ggaagaactc
    51661 aatgagctca ggcatgaggt tgaggtaacc aagtccaccg atgacattga gaaagaattt
    51721 ggcgatttac ttttctccgt ggtgaatgcc gctaggcttt atggggttga ccccgaaacc
    51781 gcgctggaac gaacaaaccg aaaatttatg aaaagattca cctacctgga acaacaaacc
    51841 atccagatgg gacgctcgct taaggatatg acccttgatg agatgaacga gatttgggaa
    51901 gaggctaaaa ggttcgatta acatataaag taaatgttcc tataccttgt tcgaaaattg
    51961 ttttacacgt tcctggtgat ttttggcgtt gccagtctgg ttttcattct ttttaacctt
    52021 attcctggcg atcccgctcg catggttctt ggccaacgaa ccgattcgct atcggtaaag
    52081 gcagcacgta ccgatttagg cctcgacctt ccggtttgga agcagtactt gaagtattta
    52141 aacgacattt cgccgctatc ggtgcataat ccaagtaata gtaaaagttc catttacctc
    52201 gacacactta tttatgggaa acccatagtg cttgggaaaa tggcatcgaa cagggtattg
    52261 gtagcaaaac agccatacct tcggcgttca taccaaagtc gtcaacctgt ttccacagtt
    52321 gttatgtcaa ctctgcccaa cacattctat cttgcactgg tggcaatagt ctttgcaaca
    52381 attgttggaa ttgcaatggg gataactgcc gcaatttaca aggatagcgt ttgggatagg
    52441 ttgctgcttg ttaattctgc gctaggtatg gctttgccat ctttctttgc tgccatactt
    52501 ataggttggc tttttgccat agtgcttggc aagtatactg gacttaacct taccggtaat
    52561 ctttgggagg ttgatgattt aggggaagga attcatctta aatggcaaaa catcattttg
    52621 cctgcattaa ctcttggctt acgtccactt ggcataatca cccagctaat gcgcagttca
    52681 ttgctggaaa cattaagcca ggactatatc agaacagcta aagcaaaggg attgccgaat
    52741 aggaaggtga tctttaaaca tgctctccgt aattccctta accctgttgt aacatccatt
    52801 tcgggttggt ttgcctcaat gatggctggt gttgtttttg ttgagtatat ctttagctgg
    52861 aaaggacttg gttatgttat ggtaaacgcg cttaatagtt acgatgtgcc ccttgttatt
    52921 ggctgcgttc ttacggttgc tgtaattttt agcatagtaa acctgttggt cgatatacta
    52981 tatggtattc tagacccgag ggtcagaatc ggctgatgta tcggaatttt gttgtaaatt
    53041 tgatagcgaa aggttaatta atgcttgaat gtttatactt aatttaaatc gattgcgatg
    53101 agaagaaaaa ttgttgctgg gaactggaag atgaatacat tgccttccga aggtttggct
    53161 cttgccaagg atgttatagc aaaatcagct gaggtttcaa gcggtgttga gctggtagta
    53221 atcccaccat tcactcacct gaacgaggta acaaaactag ccagcggaac ccgtgttgca
    53281 gtgggagccc agaattgtgc acgctggcaa tcgggtgctt acaccggcga ggtgtcagct
    53341 gccatgcttg gcgctatggg agttgagtat gtaattatcg gacattcaga aaggcgcgaa
    53401 tactttggtg aaaccaattc ccagctttac gataagattc agcaagccct ttcaagcaat
    53461 ttgatgccca ttttttgctg tggcgaaaaa ctggatgaac gcgaagccaa tcaacatttt
    53521 caggttgtta agcaacaggt tgaggaatcg ctatttgcgc tcgatgagca aagcattaag
    53581 caggttgtta ttgcttacga acccgtttgg gcaattggca ctggtagaac tgccactgcc
    53641 gaacaggcac aggaaatgca tgcctacatc cgtaaactgc ttgctggcaa gtatggctct
    53701 caaattgctg actctattag catcctttac ggaggtagct gtaaaccgtc caatgccgct
    53761 gaaatttttg ctaaacccga tgttgatggc ggattaattg ggggcgcctc attggttgca
    53821 gctgacttta ttgctattgc aaaatcgttc taggatgggt tacaccgaac tcaaaataag
    53881 gatcaaacca tacacagccg aaacagctga aattctcatt gcccaacttg ccgagttggg
    53941 ctttgagagc ttcatggaaa atgaacccga actactggcg tacattaata ctgcaactct
    54001 aaaaaaatta cctgccgatt ttattggcct tattgagttg cctgagggtg ttagaattga
    54061 ttatgagctg aacagtattg ccgacaagaa ttggaacgag gtgtgggagt cgaattttga
    54121 gccaatcatt gtcgataaca gatgtttggt taaggcatcg ttccataccg ataccccaaa
    54181 aaccgattac gaaatagtaa tcgacccaaa gatgtcgttt ggaacgggtc accatcaaac
    54241 cacacacttg atgattgagg cgcttctcaa ccatcaggtt gaggggctta ccgttctcga
    54301 catgggttgt ggaacaggtg tgcttgccat acttgccgaa atgcgcggtg cgcaaaacat
    54361 tgtggctatc gataacgatg aatgggctta ccgcaacacg ctggaaaaca ttgacaataa
    54421 tagctgcaaa cgcatcacgg cctatctagg cgatgcagag ctgcttaaag gaagatcatt
    54481 cgatttgata cttgccaata tcaaccttaa tatcctttta gccgatatgg ctgcatacgt
    54541 taacagcctt aaaagtggtg gagttatatt catgagtggc attttaaggg atgatgttga
    54601 aacgctcagg aatgctgccg aaaggttagg cctatcgttt agtgaggtta attataggga
    54661 taattgggcc atggttgctt tccgtaagta gtttctatta tgtatcggct ttcatatggc
    54721 taaaccttat gtatatagaa gaatattgat aattgcgctg ctgcttaccg catttagcct
    54781 tctgtatgct gtgttcatta gccaggacaa gaagttttcc ggtcaggtac ccctttttcc
    54841 tgcggaactt aattcgcttg taagcgataa agttaaggag caggaagagt tttttttaaa
    54901 ggggttcccg gcattttggc tttccgatac tatcgaaata tcaaaaaaag aggcaattat
    54961 tggtacggcc aatcagattc tcgaagtttt gcccgacgat aagtcttata tactaagcta
    55021 tgtaaagctc atagcagatt tttaccgcaa ccagtatgca aaaaatcagt tcgaaacatg
    55081 gtttagctac ttaaacatgg cattggctga tggatttatt aaatccgata atctccgaac
    55141 attgattcgt ttttctggtt cgcttatcga tagttcgttt atttccataa gtacttccca
    55201 tacatggaag gtattacctg caaattcgta taccttcacc ttaaaaaata atgatttgct
    55261 ggttagtttc cagaatgtca acctaatttg caggaacaac cgcaccgata gtatttttat
    55321 aagaggaaca tccggtaatc tggatataat taagcagtcg tgggagggga aaaacggtaa
    55381 ggttacatgg attcgttcaa aattcgacga atcaaaaatt tttgtggcac tcaataggta
    55441 cagaattgag cttaggcaaa gtgaatatac agccgattct gtattgttaa attatcctga
    55501 gtacttcaag aaacctattc ttggcagaat agtggataag gttacaccaa tataccagtc
    55561 gggaatagtt gattaccctg agtttaaccc ctaccagaaa tggtttgagg ttaagaatat
    55621 ctttaaggat gttgactatt caggtaattt ccgtattaac ggttccaaac tagttggaat
    55681 tggccccgat ggtagtttgg ccaaggtaac aattcaccga aagggaaaac cctttatggt
    55741 ggccgagggg cgaatggttt taattgagca gtcgcactta agctcggata aagccaaggt
    55801 tgttttttac attgataaag actcgattta ccataacggt ttgtcgtttg cataccataa
    55861 tcctacaaga actgtaatgg taaatccaac cgataaacta accactcaaa gtcctttcta
    55921 cagctcatac cataaaataa acctttggtc aaaccagcta acatggagta ttgaaaagga
    55981 tgaaattacc tttggtagca gcttgggcgc ttcaatatca aaggctgagt ttgagtcgga
    56041 gaactttttt aatcaagaca tgttcgatag catgatggat gccagcgagt tccatcctgt
    56101 gcttaccgtt tggaactata ctcgtaggat taaaagtaac acttttttgg catccgattt
    56161 ggctgtgcat gtgcgcagag ctcccgagga cgttaaaata gccatgatgc gcttagccaa
    56221 gttagggtat gtgctttaca attttgaaac cgacgaggtt accatcaccg ataagttaaa
    56281 gtataatgtg atggcccgtt ttggccgaac cgattttgat gtcatcaggt ttcaatccac
    56341 tactcccggt acacagccca atgctcgcct cgatttaaat aacttaaatc ttgccattga
    56401 gggggtaaac tacatttctg tgagcgattc gcaaaacgta tttatttccc cgtacaagaa
    56461 aagtattacc ttccagaaaa acaggaattt tgagtttgga ggtagcatta gggctggttt
    56521 atttaccttt tacggtaagg cattcaggtt tgactattcg cagtttaagg ttgagttgaa
    56581 taaagttgat tcgctgatta ttgattacca aaccgattac cgcgataact acggtcgaag
    56641 gatattgcag ggagttgcca atgcgcttca cataatctca ggcgatatac ttattgataa
    56701 accctacaat aagtcgggta gggagtacaa cccgcagtac cccatattca actgtaccag
    56761 caagtcttac gtttactacg atgcatctta catatacggg ggaatttata aacgtgattc
    56821 cttttacttt gaaattcagc cttttgttta ccaaagcatg gacaactttg aaaaggccga
    56881 catgaatttc aagggaattc tgtactccgg taatattctc gcccccattg aggaaacgct
    56941 ccgtatacgt cccgataact cccttggttt tatcactact actccttccg agggtatggc
    57001 tgtctataaa ggtaagggga gggttttcaa taaaatcgac ttaagcaatc aggggctttt
    57061 tgtaaatggt agtattgatt atattacctc aactacacag tccgataaaa tgcttctttt
    57121 ccccgactca atggttacgg tatcaacggg cttttccatt gcaaaaaggg aaactggcat
    57181 tgagttccca aatgtcaagg gcaataaaca tagcatcaaa tggctaccta aggctgataa
    57241 aatgtttatt tccaaaggtg aaaaaccctt tgtgatgtac gatagcttag caacttttag
    57301 tggaaacctt ctgcttcaac ctttaggcct tactggagca ggtttgctga atgtccccaa
    57361 tggtaaactt tcctcatcgc tattcgagta ctcctcaaat gcattagccg ctaatgagtc
    57421 aacactaact attcttaaaa ctaataccga tagtgttgcc cttgaaacct caaaggttaa
    57481 agctactata aatttcgcaa acatgcttgg cgagttcagc cgagttgaca aaagcattta
    57541 tgcctcgctc gatgctttaa ggtacagcac ctatcacgat cgttttaagt gggatctcaa
    57601 aggatacaag gtttactttg aaacattaat ggctcaggaa gatgttttgg ccggaaagtt
    57661 tagggttagt aatatgatag atagggacag cataccccgt ggaaatctgt ttttcagtac
    57721 caagccggcc gaggattcac tatacttctt cgcaccaaag gctgtatata atactgatag
    57781 aatacatttg gtagccgata gtgttaagta tattgtaact gccgatgcca ctgttttccc
    57841 aaaaaataaa agggttgagg tagatgttcc caaccgtatg tataagttct ccaatgcaaa
    57901 ggtaagggct aacaatacaa cggcctacca tgtattgcat gacgctgaga tcaagattaa
    57961 cggacggaaa agctacggtg gttcggcata ctacgattac gttgatgaac gcgattcaat
    58021 ccaaaccatc tactttccaa aagtttactc tgataacaat atgaatacct atgccgaggg
    58081 taccctaaca gagcccgata gttttaggtt aagtcccaat tttgctttca ttggaggcgt
    58141 tagccttagt gctcctgaaa agttcctttt ctttaagggt ggggctaaac caatttacaa
    58201 ctgcccagga actataggcc agtgggtacg atttgaatcg caaatcaacc ccaagcgggt
    58261 tttaatccca attggtcctg atcctcgcaa cttgaacctc catcgcttaa ttagcggaac
    58321 tgccattgcc gaggattctt taaaattgta tggtggtctt ttgcgcagtc gtaaattctg
    58381 ggaggattca tcgctggtaa cagcaaccgg attcatggag ttcaacaata aaaaccgccg
    58441 gtttaccatt gcacccctat acaagttaga taatcccgat tcttctggga atagtgttag
    58501 cctccaaaag gactactgct gggcttttgg tgagggcaac gtgcggttac ccctaaagtt
    58561 aggccatgtt acctcgcagg tttacggtag tgtaatacat aaactggaca aaaatgaggc
    58621 tacaatcgat gcgattatca ggttgaactt tcatttcaac caaaaggccc ttgaggccat
    58681 ggctgctcag ctaagtggcg ccattactct cgataaggtt gatttaagcc gtaagcttta
    58741 ccgtaaaatg ctttacgatt ggctcacacc taaggagttg cccgaggcgt taaaccaact
    58801 ttcgcttttt ggttcattca cacagatgcc caaaagcctg gagagtacca ttacactttc
    58861 cgatgtaaag ttgaggtggg atcctcagaa taaatcgttt gtgtcagtcg ggaaaattgg
    58921 agtgggtact attggtaata ttcaggtaaa ccgttttgtg aatgggttca ttgagtatta
    58981 caagggacgc tttaacgata ggttaatcct ttacatccat attggcgaca acgagtatta
    59041 ctcgttctac tatgccaatg gggtaatgtt tgtcagtgca accaatcctg aattttataa
    59101 ccctattgcc tcccaaaaat cgagtgagcg cagggtaaaa ccatcaaaag gaatgatggg
    59161 ctataggtat atgattggat caaaacgtga actaaacaga gctcagctcc gctataagga
    59221 gttagccttt ggcattaagt ccgatattcc ggttgattcc gatgacgaca aaccaaataa
    59281 ggagggcaaa gaagacgatt aaaagcccct ctttttttcc caaacctcca gatcagaaat
    59341 attagaacca tcaattttta gtctaatcgc tgtttttacc tgatggaagc cactgtttcc
    59401 agccgctccc ggatttatgt atagcatatc ccattgctta tcatacttaa cacgcagtat
    59461 gtggctgtga ccacatacta agatgtttac cttgtttccc ttaaggattt ccagaacttt
    59521 ttgatcataa tgtcctggtg tacccgcaat atgggtaatc agtatgttaa cattttctgt
    59581 tgtaaacagt tgtttttcgg gataaaccag tcgggttttg taatcgtcaa tgttgccatg
    59641 aactgcacgc aagggtttta gctttaccat attatcggct agctcaagcg atccaatatc
    59701 acctgcatgc caaatctcat cgcatgtttc aaaaaaatcg gtaagccctt tgtcccaaaa
    59761 tccgtgtgta tccgatagga tgccaatagc tttcatcttt ttattctgaa attcactgac
    59821 tcaaaattaa aaacttcagt ctaaattgta aatttgcatc aacgctgatt aaataatcta
    59881 gcacttgttc tatttatgtt tttttatcaa tctgtacacc ctttgcagat ggcaatttaa
    59941 ctattaaagg ttttatacga ttttttgtta tgtttaactt gacaaaaaca attgggttca
    60001 ttaatctcaa atctcaaatc ttgaatctga aatctgaaat ctgaaatctt gaatctctaa
    60061 tcccatgcag atattttatg ccccacaaat tgaaggcgat gagtataggc tacctgagga
    60121 cgagtccaag cattgtattc gggtgctaag gatgcagcaa ggcgacgaac tttaccttac
    60181 cgatggttgc ggaaatctgc atacagcgcg tattgccgat gaaaacccca agcgatgctt
    60241 agttcaaatt gtttcaactc agcatgagta cggtaaaagg ccattcaatc tgcatatcgc
    60301 catcgcccca actaagaaca tagaaaggtt tgaatggttt ttggaaaagg ccaccgagat
    60361 tggtgttgat accattaccc ctctcctatg cgagaggtcg gagcgtaaag atattaaggt
    60421 tgaaaggttg caaaaaattg tgatttcagc catgaagcaa tcggtaaagg cctacctgcc
    60481 aataattaat agtattcaac cgttaagaaa cttcgttaaa tatacgcatc aagccgattt
    60541 aaaagcaata gctcattgta atagctggga tttgactcca tttcagcagg taatccatag
    60601 gggtaggagt gttctagttc tcattggccc cgaaggcgat ttcacaccaa ccgaagtaaa
    60661 ccttgcgctt aatagtggat tcacgggtgt aagcctaagc acatcaaggc tacgaacgga
    60721 aacggcaggc gttgtagcct gccataccgc aaacctaatc aatggcgtat aaccttatcc
    60781 cacaaataag tcgagcacat taagtgcttt actcatcgga acctgaaaaa attccgtgta
    60841 tttgcagctt aaacaggtaa aggttgtgaa ttttcgattc tgaatgttaa aaagtttagt
    60901 ggcaacactc ccagccataa aaacttcgcc catttcaaag tctttacagc cacattttgg
    60961 gcatggttgc ttggctctaa ccctcttggt tttcattgct ttggttcccc ttttctttta
    61021 aggttgattt tttaagcctt ccggctttcc ttagtgcttg gtcaataatc cattctattt
    61081 gcccattggt gctacgaaat tcgtctgctg cccatttttc cagggcagca aacttttcgg
    61141 gatcgatccg taacacaaat gttttctttt ggcccatggt ttcttactgg tgaagtgttc
    61201 ccgcatttac aactggccta gccgattcat cggagcatag cacaaccatc aagttgctaa
    61261 ccattgctgc tttcttctcc tcgtcaaggt caacaatttg tttctgcgat agctggtcaa
    61321 gcgccatttg aaccattcct acagcaccct caacaatttt gcttcgggct gccactactg
    61381 ccgttgcttg ctggcggcga agcattgccc ctgcaatctc cgatgcgtaa gcaatatagt
    61441 tgattctggc ctcaatcacc tgtatacctg ctatggttaa cctttctatt agctcgtttt
    61501 cgagcttatg gttaacaact tcgccgcctg cacgtagggt aatttcttcc tcctcgtgtt
    61561 caaagttgtc gtaggggaac attcctgcca gcttacgaag tgcagcatcg ctctgcacta
    61621 ccacaaaatg ctcatactca tcaacctcaa atgaggcttt gtatgtatcc tttacgcgcc
    61681 aaacaagcac caatccaatc attatggggt taccaacttt gtcgttaacc ttaatgggct
    61741 cactgttaaa gttgcgtgcc ctaagcgata ttcgtttttt cagatagaat gggtttgtcc
    61801 agaaaaaacc gttacgcttt atggtgccca tgtacttccc gaaaagtatc agcactgccg
    61861 attcgtttgg gtttaccact ataaacccag gcataatcag cactattggc aaaaagccaa
    61921 gagcatacca tggcgattct gttgtaatca acaggctaat ggttgctgca accagaataa
    61981 accaagccac cagcatcagg tagcccgatt tgggatgaaa ttctttttcc ataactgata
    62041 ttattaaagt gatattaaaa tgatattaca aatatattac agaattatat aaaagtcaat
    62101 aaaaaaggcc gatttttttc ggccttaatc tttaattttt gctaaaagtt gatagaattg
    62161 tttttcgggt gctggtggtg ttgccctttc gagtttttcc aggtaatcag taaaatacct
    62221 ttcaatgtta ctttgctcaa gtacattaaa tccttcaaag tcttttcggg atggacggtt
    62281 cctggattta ctatcaacat tattattttt cactgcgtag atatttacca taggcaaatt
    62341 ggttgaatgg tttcaacatc aaaacgtata tatatataag gtattgtatt aaatcgggaa
    62401 aaaattatgc aaaatgattc aatgtcgttt agctaacgaa acagaccctc cttatgtcat
    62461 cctaagggta gcgaaggatc aattcaatag tgtgcannnn nnnnnnnnnn nnnnnnnnnn
    62521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    62581 nnnnnnnnnn nnnnnntaga acttggcaag cacacctaca aggtcagatt ggtatgccat
    62641 aacagcatca atatccttgt atgccattgg cacctcatcg agccctgcac ctataagttc
    62701 cactccccat ttctccagaa tgtagttaag atcagcctga gtaaacgttt gtagcgcttt
    62761 gcttctggac atttttcggc ctgccccatg cgatgccgat gaaagcgatg ccggattacc
    62821 cttgcctttt atcacaaagg ccggatgaac cattgaacct ggaataattc ccagtgaatt
    62881 tgggtgtgcg ggcgtagcgc ctttccggtg aacaatggcc ttacggccat cagggaggct
    62941 ctcaaaccat gcaaagttat ggtgattttc aatcatcctt atgggacgta cattcagctc
    63001 acgggcaacc tttgcatgga tctcatggtg gttagccgat gcatattcgc cggctaggtt
    63061 catggcaagc cagtattcct gaccttcctg gctgttcaaa tcgagccagg ccaaatggcg
    63121 ggctttgccg ggtagcctca ccttttccat ggcaagtttt gaatagtaaa gagctatttc
    63181 gctaccaaat ccccgcgacc ctgaatgtga tagcaatgcc aggtactttc cgggttcaaa
    63241 tccaagcaac gggtcgaact cggtaatgtg caattcaccc cattccacaa agtggttacc
    63301 gccacccgat gttcctagct ggctgtaagc cagatcgcgg agctgcttta ttacctttgt
    63361 tgccgaccat tcctttttgt caaaaagcga agtgtcaata tggtttttat tcttactacc
    63421 cacgccaaaa atggtagttc ggaggaggat ttggcttaac gtttcacttt tagtacctaa
    63481 aatctcaggt ttaatcggaa aaaccgacat acacatccgg caggcaatat caactccaac
    63541 agcgtaagga ataataacgt ttccggttgt ggctaaaaca ccgccaatgg gcaaaccgta
    63601 tccttcatgg gcgtcggcca ttagtgagcc cgcaagtgta acgggcaagc tcatggcgtt
    63661 ttccatttgt tcaattgccc cgggttctat accatcggct ccataaatgg tgtagctaag
    63721 gggcttatgc aatgaaacct caccatgaag ttcattgctt tttgaatcga cactggtaat
    63781 attcttagcc ttaaggtgct gggtaagtgt tttaaaccga ttatcggtat caatcacttc
    63841 aggagtttca agagcattac agatgtattg caacagttca ccctttgtaa caagctggct
    63901 acggagtatg atgttggctt cacgacatat agcggcaata tcgaagttcc ctttcgtttt
    63961 tagaaagtga agttccttgg gttttagctt gctcttgccc a
//
LOCUS       DAOSAL010000006        60604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_394_length_60604_cov_48.057705, whole genome shotgun sequence.
ACCESSION   DAOSAL010000006 DAOSAL010000000
VERSION     DAOSAL010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 60604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 60604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60604
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_394_length_60604_cov_48.057705"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..1569)
                     /locus_tag="PK049_01715"
     CDS             complement(109..1569)
                     /locus_tag="PK049_01715"
                     /inference="COORDINATES: protein motif:HMM:NF015882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HOK60303.1"
                     /translation="MSKVNIKLERLGEMFPENWTEEQRAKGKTLFLKYLSEKAHKHYG
                     GKIQTAPKAPVLGFNWFNVWYTPGVSKISTTIRDNNDASFELSNRGNLVAVVSDSTRV
                     LGDGDCTPPGGLGVMEGKAFLMKYLGGVDAVALCIDSKGKDGKNDPEKIIDFVKMCQH
                     SFGAVNLEDISQPNCYRVLDVLREECVIPVWHDDAQGTACVTLAGLLNALKLVNKKLK
                     DVRIVFFGAGASNTTIARLMIADGGDPKKMIMFDSHGALHRKRDDIKADSRFYRKWEI
                     CEQTNPDCINTMEEAMKGADVLISLSTPGPDTIKQDWIRLMADKAIVFACANPVPEIY
                     PYAAKAAGAYIVATGRGDFPNQVNNSLGFPGILKGALLVRASKITDNMAIAAAHSLAN
                     YAEKRGINPDNIVPTMDEADVFPVEAADVAVQAVKDGVARRPMSWDEAYSIAKKDIEY
                     SRSMVKSLHDNGFITDPPTEMLEEALEWTIKQIEGK"
     gene            1768..2640
                     /gene="sucD"
                     /locus_tag="PK049_01720"
     CDS             1768..2640
                     /gene="sucD"
                     /locus_tag="PK049_01720"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HOK60304.1"
                     /translation="MSVLVNKSSRVIVQGFTGGEGTFHATQMIEYGTNVVGGVTPGKG
                     GQTHLDRPVFNTVSEAVKETGADVSVIFVPPTFAADGIMEAAAAGIKVIVCITEGIPT
                     NDMVKVKHFLADYPETRLVGPNCPGVITPEEAKVGIMPGFIHKKGHVGVVSRSGTLTY
                     EAVDQLTKLGLGQSTCIGIGGDPVIGTTTLEAVKLLMADPDTHGIVMIGEIGGSMETD
                     AARWIKEHGTKPVVGFIAGQTAPKGRRMGHAGAIIGGADDTAAAKMKIMRECGIHVVE
                     SPANIGQTMKDALK"
     assembly_gap    2754..2853
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2891..3490)
                     /locus_tag="PK049_01725"
     CDS             complement(2891..3490)
                     /locus_tag="PK049_01725"
                     /inference="COORDINATES: protein motif:HMM:NF025691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HOK60305.1"
                     /translation="MKLKVQHQERYSRLELLLRTFFGWLYILLPHGFLLFFVFIWALI
                     LQIVAFWAILFTGKYPKSLFDFQVGLFRWKLRVNARVLNISDGYPSFGINGTDEYTSL
                     EVEYPERVSRALMLVRFFFGWLYVYIPHLFILFFRQIFVGVIIILAWLIVLITGKYPR
                     EFHSWVVGQLRWMTRVGLYMGYMTDVYPAFTGDELDSEK"
     gene            3671..5062
                     /locus_tag="PK049_01730"
     CDS             3671..5062
                     /locus_tag="PK049_01730"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOK60306.1"
                     /translation="MRQKNLFSLRNILFAALIAATMVFASCKQQPKEAEPFRVEHANW
                     VYNATIYEVNVRQYTPEGTFKAFEQHLPRLKDLGVDILWFMPIHPIGVENRKGTLGSY
                     YSVKDYYGINPEFGTLDDFKSVVAKAHELGMKVIIDIVANHTSHDAVLLTEHPDWYVR
                     DSSGRVVSPFDWTDVAKLDYSKPELRRYMIDMLKYWIKDIDLDGFRCDVAAEVPTDFW
                     NEARAELNQLGKPIFMLAEAETPELQKYAFDADYAWEFHHIMNGIAQGKKSVVELEQY
                     LLKKAATYPKNTIKMNFITNHDENSWNGTEFERMGDAVKTMAALTFVIDGMPLIYTGQ
                     EVGLNHRLQFFEKDQVDWTDSKGLTDFYEKLTALKKSAKVLNAAEQGADIFRITTTAD
                     SSIFAFIRENETQKLFAVFNLSAKEQTFNFTGDAHLNTSYVEYFSNSSKEFTKDERVT
                     LKPWEFSIYLNNK"
     gene            5278..6465
                     /locus_tag="PK049_01735"
     CDS             5278..6465
                     /locus_tag="PK049_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005657367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOK60307.1"
                     /translation="MYYNIPVVGKVHWIGVNDRRKRLFENIWPLDRGVSYNSYLIVDE
                     KTALIDTIEDRAAGNYIERIKALLNGRTLDYLIINHMEPDHSGEIKDIFEQFKGVKLV
                     GNAKTFKMVEAYWGINENLIQVDDGDTLDLGFHKLKFVMTPWVHWPETMMTFDQTEQI
                     LFSGDAFGSFGALDGGIFDNEINFDFFEDEMRRYYSNIVGKYSNMVQKAFAKLKDVPV
                     KVIAPTHGPVWRSNPNKVIELYDRWSRYEAEEGVVIIFASMYGNTEAIADYIGRKVAE
                     NGIKNIRIHDISRTHISHLINEIWKYRGLVLGSCAYNSEMFPLMEQLTRELTHMGIKN
                     KKLALFGSYSWNGGGVKNLLKFAEEAKLELVAEPAEIYGKPNAEKLKQFDLLAQKIAE
                     SVK"
     gene            complement(6563..6832)
                     /locus_tag="PK049_01740"
     CDS             complement(6563..6832)
                     /locus_tag="PK049_01740"
                     /inference="COORDINATES: protein motif:HMM:NF017690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOK60308.1"
                     /translation="MSKVIIEKLTDEQISKMGIKNWPIWTKEISTFDWYYDTDEECLI
                     LDGEVDVKTDEGTYTVRTGDFVTFKKGLKCVWTVKKPIRKHYNFK"
     gene            6928..7317
                     /locus_tag="PK049_01745"
     CDS             6928..7317
                     /locus_tag="PK049_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOK60309.1"
                     /translation="MEFNIPLGIEFTATEMVTKDNTASKYGSGLVDVFATPAMVALME
                     KAALNAVLPHLPYGFNTVGTEICVKHTKATPLGWEVHSKATLKEVDGKKLVFDVVAWD
                     SEGEIGRGTHTRYIIDSKRFMEKFSSK"
     gene            7327..8085
                     /locus_tag="PK049_01750"
     CDS             7327..8085
                     /locus_tag="PK049_01750"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HOK60310.1"
                     /translation="MNFDTYQFPKYHRHKLKVHSYEVDFNQRLTVTALFNYLQEIAWE
                     HAHLLGYGWEHLLQKGWFWALSRVEAEIYRLPAWTDEVVLLTWPRGVDGIFALRDFEV
                     YDHLGNTIIAASSSWLVLNIQTRRPVRAEWYSDFDYATRSSLGRNATKLVEQSVSPEF
                     SEKFDVRIGDIDMNQHVNNVRYIDWAYNTFSIEHFKSYFPKRVVVNFNAEGKANDSII
                     ASRFQNSQDESIVEIANASNGKNLCRLMFEWGKI"
     gene            8158..9699
                     /locus_tag="PK049_01755"
     CDS             8158..9699
                     /locus_tag="PK049_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HOK60311.1"
                     /translation="MYSQQDIVQMQRKGITPEMVEQQIQSFKKGFPYMKLVKPATVGN
                     GIIRLDGEELDSLIEMYRDFEGSRVKFVPASGAATRMFKHLYEFQSDYPVKGLECFND
                     KGFNTVFTFFERIKEFPFFDKLYNELWMQGYKMDELIDSKDYMPIVNTLLGSEGLNYG
                     NLPKALILFHKYGDIARTAMEEHLVEGAQYARDQHGNVNIHLTVSPEHIAGFEQLIER
                     VKSYYENHYEVTLNITYSVQKPSTDTIAVDMNNNPFRNDDGTLLFRPGGHGALIENLN
                     DLGHELVFIKNIDNVAPDHLKPLTVRYKKALAGMLLSYRGIIYKYLQELDSQNLSVDE
                     LLAILDFTRDELCVIPPASLNTSDPAELNKYLFSVLNRPIRVCGMVKNEGEPGGGPFW
                     AVNPDGTTSLQIVESSQIDMNDPTSKAILNASTHFNPVDIVCSFIDFKGNKFNLLNYR
                     DPQTGFISVKSKDGKQLKALELPGLWNGAMSNWNTIFVEVPLATFSPVKTVNDLLREE
                     HQPCR"
     gene            complement(9705..10460)
                     /gene="truA"
                     /locus_tag="PK049_01760"
     CDS             complement(9705..10460)
                     /gene="truA"
                     /locus_tag="PK049_01760"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOK60312.1"
                     /translation="MAINRYFIYLSYRGTNYHGWQIQENATSVQNLVNNALSILLKEN
                     IQTIGAGRTDTGVHARFYVAHFDSVASNLESDGKFLHSLNRILPNDIAIHQIKKVNPS
                     ANARFDATLRGYEYVICRNKDPFWIGLSWFYDAQLNIDNMQLAAQQLMNYTDFTSFSK
                     VGSETKTNNCQIYKAEWNESGNLLIFTIEANRFLRNMVRAIVGTLIDVGRGKITPEEF
                     AQVIEKRDRKLAGTSAPAEGLYLTRIEYPDNLI"
     assembly_gap    10758..10857
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11099..12100
                     /locus_tag="PK049_01765"
     CDS             11099..12100
                     /locus_tag="PK049_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOK60313.1"
                     /translation="MSVQSTLDIKELNERIRLESSFVDIINIEMGKVIVGQKHLVESL
                     LVGLLADGHILLEGVPGLAKTLAINTLANIIDVKFNRIQFTPDLLPADLIGTLIYSPK
                     SEQFTVKKGPIFANFILADEINRAPAKVQSALLEAMQERQVTIGDETYKLEEPFLVLA
                     TQNPIEQEGTYPLPEAQVDRFMLKVKIGYPKLEEERLIMRENLAMQFPKPEKVLKPTD
                     IVKARQVVREVYMDEKIEKYILDIVFATRYPAQYNLQRFEPMISYGASPRASISLGLA
                     SKAYAFIKRRGYVIPEDVRAVCFDVLRHRIGLTYEAEAENITSEDIITEILNTIEVP"
     gene            12106..12525
                     /locus_tag="PK049_01770"
     CDS             12106..12525
                     /locus_tag="PK049_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60314.1"
                     /translation="MKPLAIIFLSVAVAFLSLSVSAQNYISLHKDEIAKRMPQENKGF
                     YFEKEVNVDDRGFLKYVNTLDEQTILFMIDTDGFCTAVSRMYNTWLYDQVVNELNSKY
                     QQVSTNKWLEVKEGKSYDLTLIKGKWFLTLTIRPHSK"
     gene            12534..13424
                     /locus_tag="PK049_01775"
     CDS             12534..13424
                     /locus_tag="PK049_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOK60315.1"
                     /translation="METAELLKKVRRIEIKTRGLTRQIFAGQYHSAFKGRGMAFSEVR
                     EYRVGDDIRSIDWNVTARYNQPYVKVFEEERELTVMLLIDVSGSQNFGSTQHFKRHVI
                     AEVAAVLAFSAIQNNDKIGVILFSDKVEKFIPPQKGSKHILRIISELLNFEPSGKETN
                     IAEALRFLTNAIKKRSTAFVLSDFIDFNEQNNQPRFTDAISIANNKHDVVAIRIYDDR
                     ETQLPSVGLVRFTDAETGNDIWIDTANKLVQNHYSQWWNITTENLKQTFTKAKVDWVS
                     VRTDEDYVKPLMLLFKRRSK"
     gene            13421..14425
                     /locus_tag="PK049_01780"
     CDS             13421..14425
                     /locus_tag="PK049_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60316.1"
                     /translation="MKLIRFVFIVFLLLTLNVDAQIEQLEIKAVLKADTILIGDRVEF
                     AIIANGKGNFSVSLPVLADTLPGGIYIIGKPIIDSTIKDNTRSYSFKLPVTAFSPGNV
                     SVSKFPVLVKHGGRVDTTWITTDSLFVKLVPRDTTLKDIKDIKPPIKEPIRFSEVAPW
                     AGLGLLFAAIIVLLVMYLRSRKQNKPFLNIFKPKEPAHVVALRELMLLEENRDWASDN
                     PKLFYTKLIDILRNYLEGRFQINAPEQTTSEILSEIDKLDFDFSPFIETLRELLFTSD
                     LVKFAKHVTTIDENRSFLSFAINFVNSTKPIESSNDGENTTDEIKSADNTDLLTSDSN
                     "
     gene            14432..15421
                     /locus_tag="PK049_01785"
     CDS             14432..15421
                     /locus_tag="PK049_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOK60317.1"
                     /translation="MLLVVYANPKLLYLLLLIPVLVGWYIYRLHKSRAALIISTAKHL
                     DNTGKSVKVYLRHFPFALRMLALTLAIIALARPQSTNIQRNVTTEGIDIVLSLDVSGS
                     MLARDFKPDRLEAAKSLGINFISGRANDRIGLVIFAGESFTLCPLTTDRATLINQFKA
                     VDIGVLEDGTAIGSGLATAISRIKDSDAKSKVIILLTDGVNNRGEIAPMTAAEMAKTF
                     GIRVYTIGVGTYGTAPYPVQTPFGTRYQDVQVEIDEDLLKGIAQMTGGEYFRATNNKA
                     LEQVYQKIDQLEKSKIEVNEYAKREENYRGWLLAAFILLLLEFVLRRTYLLGI"
     gene            15435..16478
                     /locus_tag="PK049_01790"
     CDS             15435..16478
                     /locus_tag="PK049_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOK60318.1"
                     /translation="MFRFSNPEYLYLLLLIPVIIGLFWYSVRKQKQWINSFGIYKTIL
                     KLMPGHSFNRYWIKFIIATAAYIFIIIALAGPQVGAKLTEVKRKGMEMIIALDVSNSM
                     LAQDIKPNRIERAKQAISQLIDRFNNDRIGLIVFAGDAYIQLPVTNDYASAKLFLSSI
                     NPGIVPKQGTAIGRAIELAVNSFSPNANTGKVIALISDGENHTDDPIEAAAMAAENGI
                     SIYTIGIGSPEGAPIPLGNNQEFLKDADGKVVITRLDEETLTKIAVTANGKYVRASNT
                     QFGLLPIYESLKKVDRKAIKDKVYSEYNEQYQYILAIAIALLFAELIILDRKTSLLSK
                     VNLFGTDKTREEL"
     gene            16475..17206
                     /locus_tag="PK049_01795"
     CDS             16475..17206
                     /locus_tag="PK049_01795"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOK60319.1"
                     /translation="MRKDIVLIILLAGLTVNTFAQSEHKLIRRGNRAYSKNDYLESEV
                     QYREALERNKHSFKANFNLGDALYKQNKFSDSDSIFNGINTSALSDADKSMIYYNKGN
                     SLFKQNKFKESADAYKMALKYNPNDIDAKYNLSEALRMMQNQQNQNNNQQQNNSNDSK
                     DNKDQKDDKKQDKKEDKKDNNNNKQDNQQNNQNKNNQQQQQPKISKEDAERMLQALQQ
                     REKDIQDKLNKKQAKPVSGASIKNW"
     gene            17212..>17477
                     /locus_tag="PK049_01800"
     CDS             17212..>17477
                     /locus_tag="PK049_01800"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HOK60320.1"
                     /translation="MKRYLSGILLLFISGYLQSQEVTLQAYAPNLVEVGEQFSLSFTL
                     NAKPSEFKAPSIVDFDILAGPSTSSSTSIEVINGKVTQSQSYTYT"
     assembly_gap    17478..17577
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17578..18463
                     /locus_tag="PK049_01805"
     CDS             <17578..18463
                     /locus_tag="PK049_01805"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HOK60321.1"
                     /translation="LKANDAFTYRLKISGKGNLKLIGNPKLNFPSTFEVFDPKISENI
                     SVDEGNAMGSKTYEYVCIPRAGGNFSIEPAVFSYFDPDKESYITLKTSAISVAVSADS
                     SSTGNVMVASFGKEDIKFIGKDIRFIKTSSSAFKSKSAFWVTSGYYQLLYILLIALFI
                     TFSYLFRRYRQRMQDVAFVKTRRASKVAQKRLKMASQLLEQNNNAQFFEEIHKAIWGY
                     VSDKLNIPLASLSLDNASEKLTQQGVDADRISTFRSIVETCEYARFAPMAEHSQMTDI
                     YEKTFSLIEDLEDTLKRK"
     gene            18489..19253
                     /locus_tag="PK049_01810"
     CDS             18489..19253
                     /locus_tag="PK049_01810"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOK60322.1"
                     /translation="MKRILYLALMIVPVSLFGKVTMQNPDSLFALANKYYTEGKYSEA
                     VTHYEAIVANGKVSAELYFNLGNAYFKLNDIAHAILNFERAYLLNPGDDDISFNLELA
                     RAYTTDKIEAIPDFFVVKWVKSISNLFSSNTWAFLAISSFIIALILFMIFQFSGKYLI
                     KKYAFIISWFSAFIFIVSLAFSVSQKNRIVNSNHAIVTSSAVSVKSSPSESSNDLFIL
                     HAGTKVETLRNVGEWCEIRIADGNKGWLPKSTFERI"
     gene            19483..20025
                     /locus_tag="PK049_01815"
     CDS             19483..20025
                     /locus_tag="PK049_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HOK60323.1"
                     /translation="MKILGMIILLATTLSTTNAQNVSFHSFVVTDIDGNEFPLSKLKG
                     KKVMVVNTASKCGFTPQYEDLQALYDKYKDKNFVIIGFPANNFMGQEPGTNQEIKEFC
                     TTRFSVSFPMMAKISVKGNDTHPLYQWLTQKSMNGVMDSEVKWNFQKYLIDENGKLVD
                     MIPPKEKPNSDKVIKWIEGN"
     gene            complement(20041..21528)
                     /locus_tag="PK049_01820"
     CDS             complement(20041..21528)
                     /locus_tag="PK049_01820"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOK60324.1"
                     /translation="MWKYLVFLVGILVTHDLAISQTKIRQSPIDSKDLMNTVKFLSSE
                     RFKGRLPGSVEYEQASRYVATRFKNAGLNPLLGNTMLQEFDEEVNYILDARVFLLDQN
                     NRPTQPMYLGEDFVCRGFTGSGEVRGETVFAGFGIKNDEYNDYRSIDVRNKIVIVFKS
                     TPPWQASSGSWGDVSPRGKARIAKELGAKAIIFIGEPHMTPNTMVYGSIACGPKPHLA
                     DFPMIHAGSRFTDSLFSKLPYKPADFYEMIKRDKAPHSVETDKSIYINVRAVYQEAKK
                     TFNVVGYIEGSDPKLKHEYIILGAHLDHVGYQGDKLYFPGANDNASGVAGLIAIAEAI
                     NKSTTKPLRSVVFVAFSSEESGLKGSKYFVANMPINADKVVAMLNFDCIGQGDSIAIG
                     GRLSYPKLWKKAKKIDKRNTKLLSPKTFGGGGADAQPFYEAGIPTLYFNTSGGYKYLH
                     QATDKPETLNPEVFEKVVHLGYLTTMELANKNYKREKDRQKKKAS"
     gene            21617..22204
                     /locus_tag="PK049_01825"
     CDS             21617..22204
                     /locus_tag="PK049_01825"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HOK60325.1"
                     /translation="MAPDFLQSDLYLYGVLPLLIFFARICDVTIGTLRIIYVSKGQRR
                     IAPILGFFEVFIWIVAISQIMKNLNNFACYFGYAAGFATGNYIGMLVEERLAFGKVLV
                     RVFSTDNGADLVQILNASGFGATSFQAQGAQGEVSIIYSVINRKNLPEVQSLLLNFNP
                     NLFYVVEDIRKVNKGIFQATAQPKISIFERWRKGK"
     gene            complement(22206..22550)
                     /locus_tag="PK049_01830"
     CDS             complement(22206..22550)
                     /locus_tag="PK049_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018669580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOK60326.1"
                     /translation="MNTDLLSWPNGVDFAITICDTAGTILYMNEKSQKVFERWGGKEL
                     IGKNLFDCHSDNSVRIIKELMATGKSNTYTIEKNGVKKLIHQTPWYSNGRVAGMVEIS
                     IETPEGMPHRVR"
     gene            23083..24252
                     /locus_tag="PK049_01835"
     CDS             23083..24252
                     /locus_tag="PK049_01835"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOK60327.1"
                     /translation="MKKVNSLRTPIYRDSGFWLKDIPTMKKAFADEVNHPADPDLYIY
                     SRYRNPTVVQTEQELAKIEGSAWALLAQSGLAAIDIALSIFQVNAQPVKWLFFSEIYG
                     GTNTFIDKVLVARRGFNIVRFTPTNDSYSLSQFERLMEEQKPDVVFLEAISNPMLIVA
                     DVPEIVKISKRFGAKVIVDNTFATPYILKPLDFGADLVIHSATKYLAGHGNLSAGVVC
                     GNDIDLLKQAIEYRKLVGHMISPDDAYRLSDYLKSFHLRISQHFSNAQRIAEFLSKHP
                     AVEKVLYPSMPYHPTSNIASKIFGNGYGGIVTFDIKGNSFTHKQENCNTFIQLVSHNI
                     HLIPTLGDADTILMPVEPVWGDKYPFPGMVRLSIGIELPESLINVLKNALDKISC"
     gene            24512..25129
                     /locus_tag="PK049_01840"
     CDS             24512..25129
                     /locus_tag="PK049_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60328.1"
                     /translation="MQNKLQELTEKIYREGLEKGQAEANQLVTKAKDEAARIVANAKA
                     EAERIIAAARKEADDYRKNIDTEVALSAKQVISGVKQQIVSLIETKLYEAPLVDALKD
                     ADFVKKVIETAINRWEPNSAESVSLRVLVPEEMAKTITDHFTTKTHALLNKEVEIQVD
                     RAIKYGFRIGPKDGSYVVSFTETDFENLFKDFMRPKIVAMLFGGK"
     gene            25116..25994
                     /locus_tag="PK049_01845"
     CDS             25116..25994
                     /locus_tag="PK049_01845"
                     /inference="COORDINATES: protein motif:HMM:NF022415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2764 family protein"
                     /protein_id="HOK60329.1"
                     /translation="MEVSRMMRNYYYLVAGLPELIMDQDRKDFSVNALKQEVKEQVSS
                     SDYRLVEFLHLPYDNDNFLNKLLNRGLEFNDLGSYEKFVFDELDENINQLPQYMQDFY
                     YRFTGKQRSADDAVDQDEQPKDIFEVEKLPEIRFLESFYDLALSQNNDFIRYWFSFTR
                     DFNNVLTALNCRRLGVDPATQLVGNNWLTEVLVKSQAADFGLKREVDYIDRLIQATDI
                     SDVLERERKLDLIKWDMSVEFTTWDYFNINFILGFFVRAGIVHRWLKLDAKTGEELFR
                     KLFDDLKSSYNLSEKF"
     gene            26022..27779
                     /locus_tag="PK049_01850"
     CDS             26022..27779
                     /locus_tag="PK049_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOK60330.1"
                     /translation="MKTQGKVTGIISNLVVVEVDGPVAQNEICYINLAGTKLMAEVIK
                     VIGKLAYVQVFESTRGLRVGTPVEFQGHMLEVTLGPGLLSKNYDGLQHDLDKMKGIFL
                     QRGDYTDALDFDSEWDFTPLVEPGAQVSAADWLGEVPENWVKHKIMVPFTFTDKYTVK
                     SIVPAGKYKVSDSIAVLVDSKGEEHKVTMYQKWPVKKAITAYKEKPRPFKILETGVRV
                     IDTLNPIAEGGTGFIPGPFGCGKTVLQHALAKQANADMIIMAACGERANEVVEIFTEF
                     PHLDDPRTGRKLMERTTIICNTSNMPVAAREASVYTAMTIAEYYRSMGLKVLLLADST
                     SRWAQALREMSNRLEELPGPDAFPMDLPAVIANFYARAGFVYLNNGSTGSVTFIGTVS
                     PAGGNLKEPVTESTKKAARCFYALSQQRADSKRYPAIDPMDSYSKYLEYPEIQEFLNK
                     NVNETWVDMVMKTKNIIQRGREAFEQINILGDDGVPLDYHDRYWKAEIIDFVVLQQDA
                     FDKIDCNTPLKRQRYMLEKVMQICDTNFHFDGFEQCSSYYKRIINALKQMNYSEFQSE
                     EFYNYEKELETILNERRVQ"
     gene            27779..29101
                     /locus_tag="PK049_01855"
     CDS             27779..29101
                     /locus_tag="PK049_01855"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOK60331.1"
                     /translation="METKAFQRIYTQLTGITKATVSLRAQGVANEELAMVHGRLAQVV
                     KIMGDMVTLQVFSGTEGIPTNAEVVFFGEPPKLKVGDDLAGRFFDAYGRPMFGDSEIE
                     GEPREIGGPSVNPLRRKQPSELIATGIAGIDLNNTLVTGQKIPFFADPDQPFNQVMAM
                     VALRAQADKIILGGMGLSNDDFLFFKNVFENAGALDRIISFVNTTEQPPVERLLVPDM
                     ALTAAEYFAVEKKEKVLVLLTDMTLYADALSIVSNRMDQIPSKDSMPGSLYSDLAKIY
                     EKAVQFPDGGSITIIAVTTLSGGDITHAIPDNTGYITEGQLFLRTDSDTGKVIVDPFR
                     SLSRLKQLVIGKKTREDHPQVMNAAVRLYADAANAKTKLENGFDLTEYDERALKFAHD
                     YSEKLLAIDVNVEVDTMLNTAWQLFAKYFSKTEVAIKNELVEKYWPAN"
     gene            29117..29719
                     /locus_tag="PK049_01860"
     CDS             29117..29719
                     /locus_tag="PK049_01860"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOK60332.1"
                     /translation="MAIKFQFNKTSLNELNKQLKIRQRALPTLKNKESALRMEVKRAK
                     NRMQELEEHLAKRIKDYEYMVSLWGEFERNLIQIDDVRLGTIKIAGVKIPVLQEVIFS
                     VKEYSVFARPYWYTDGVKILKELAEIGIERDFYHEKMRLLDHARKKTTQKVNLYEKVQ
                     IPGYENAIRKIKRFLEDEENLSKSAMKIVKKRYENVEVES"
     gene            29716..31527
                     /locus_tag="PK049_01865"
     CDS             29716..31527
                     /locus_tag="PK049_01865"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HOK60333.1"
                     /translation="MITPMYKYNFLVYHRDFDDFLLKLQDLGVVDISMENRNVDEDEK
                     EILARINRIATAIKFLEGRKAPDEKITLSLSADEIVSRVELLQREREQFENAIKKAQK
                     ELAEVEPWGNFDLKLLNSLAEKGISIRFFIAPEKVFNEDWVNEYPIEIVNHTNGLYYF
                     VMVNYTGEAISPDVQEVRTPQFSVKQKDEEIHRYHKRIDEINTELDFLALYIDLLKKE
                     QIELTNQLDFKMVKSSATAEAGNTIMVLEGWVPEDLNEKVKEVLDAESIIYISSKADK
                     NDNAPILLRNSKFAKLFEPISKLFAFPTYAELDLTPFFAPFFMLFFGFCFGDAGYGLI
                     FVLGATIYKFKAKPELKPLLSLIQILGISTVLFGALTGTVFGASLVELDIPALESFKS
                     YFFDQDKMFKLSLILGLVQIVFGMCVKAANLWVQFSWKHAISTISWVVLILSMAVFYL
                     LGQNPESSLKLFSPIHMVIVGVAGVGIMFFNSPGKNPLFNFGLGLWDTYNMATGLLGD
                     TLSYIRLFALGLSSGILGSVFNSLAFGMAPDTPVLKHIVIILILLIGHSINIFMSALG
                     SLVHPMRLTFVEFYKNAGFTGGGRGYNPFRKNTAEAQ"
     gene            31574..32020
                     /locus_tag="PK049_01870"
     CDS             31574..32020
                     /locus_tag="PK049_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HOK60334.1"
                     /translation="MEPIIFAYLGIGIMIILSGVGSAYGVTIAGNASVGALKKEPGAF
                     GNFLVLSALAGTQGLYGFTGYFMLKKFLIPEITWAQAAAIFGAGLAMGFVGLFSAIRQ
                     GQVCANGITAIGNGHKVFVNTLILAVFPELYAIISLAAVFLISTSL"
     gene            complement(32035..33816)
                     /locus_tag="PK049_01875"
     CDS             complement(32035..33816)
                     /locus_tag="PK049_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOK60335.1"
                     /translation="MGFLPTSVKEIKALGWEQPDIIIFTGDAYVDHPAFGAAVIGRVL
                     ESEGFKVAIVPQPNWRDDLRDFKKLGEPRLFFAVTAGNMDSMVNHYTAAKRLRSDDAY
                     TPDGKAGFRPDYATVVYSRIIKKLYASTPLIIGGVEASLRRLTHYDYWSDTLKPSILV
                     DSQADILVYGMGEKAIIEIAKKLDQGMAIESLRGIPQVAYIDHNIDKYKRGEDILILS
                     SYEECLADKLKFANNFKIIEQESNRINSKILIEPIEDRCVVVNPPFNPATTHEIDRWY
                     DLPYTRLPHPRYKGKTIPAWEMIKFSVNIHRGCFGGCSFCTISAHQGRFISSRSEQSI
                     LKEIEEIKKIPGFAGYLSDLGGPSANMYGMGPKDLSICAKCKRPSCIYPNICKNLNSS
                     HEPINNLYNQVRKVEGIKKAFVGSGVRYDLFITRKDSKAEREYFENLFLHHVSGRLKV
                     APEHTSEKVLKIMRKPSFELFKQLKQWFDALNEKFGQKQQLIPYFISSHPGSTEQDMK
                     QLAEETRKLNFKLEQVQDFTPTPMTLASTIYYTGINPYTGEKVYVARSVEEKRKQQSY
                     FFWYKNESKSLFTQSKKRPPKRKPPIR"
     gene            complement(33818..35032)
                     /locus_tag="PK049_01880"
     CDS             complement(33818..35032)
                     /locus_tag="PK049_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013619142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HOK60336.1"
                     /translation="MKVKKISITDYDYELPDSRIAKHPLAERDCSKFLIYQNGEITND
                     TFRNINQYITPKSLLILNNTKVVRARLRFKKESGATIEVFLIEPIEPSDYALSFSSKK
                     KVVWKCIVGNLKRWKQGELQMEIEGTGGRLVAKQLKRLSEGVEVEFLWNNSSMSFADI
                     VEKCGTMPIPPYLNRESEDIDGYRYQTIYAEPEGSVAAPTAGLHFTPKVFESLSKIHV
                     KPHYITLHVGAGTFKPVTSDTIEGHEMHTEHFFVSLELVKRLYQNSEPVVCVGTTTLR
                     TLESLYWLGVKLKLGKLNGNFNISQWEPYEFDFQIDVGEAYKALYEHMKNNGIETLSA
                     STQIIIAPGYEIKTCDALITNFHQPRSTLLLLVAAAVGNDWKKIYQFALDNNFRFLSY
                     GDSSLLHINKST"
     gene            35116..36177
                     /locus_tag="PK049_01885"
     CDS             35116..36177
                     /locus_tag="PK049_01885"
                     /inference="COORDINATES: protein motif:HMM:NF021179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2027 domain-containing protein"
                     /protein_id="HOK60337.1"
                     /translation="MNVAVGCKVRFLNDVGGGEVIRIIDKRTALVKTFDGFEMPVLLS
                     ELVVVEPAADELLQKKTVQEKETADANRNKKPKSEVINLTDDTPEVADSSGDELSLDI
                     AFVPSNISNLQQSTFDMYLVNDSTYRVFYVLSNVIDDNHTPISSGILQPDTKVLVKRL
                     TPEDVSKLILMNLQAIYYKNIPYKPFQPEFYDIELDPIKLYKRGNFKRNDFFDIDAYI
                     ISIASTKREAFIRHLTDKAIVEAIKQKEKPVQVKEVKKPNPDTEEVDLHIHELIDNWK
                     DLTPGEILKIQLARFETALEGGIKSTTTKRMVFIHGVGNGKLKYEITKLLSSKYPKLR
                     YQDASFKEYGFGATMVFLK"
     gene            36219..36598
                     /gene="rnpB"
                     /locus_tag="PK049_01890"
     ncRNA           36219..36598
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PK049_01890"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            37758..38183
                     /locus_tag="PK049_01895"
     CDS             37758..38183
                     /locus_tag="PK049_01895"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOK60338.1"
                     /translation="MVNERLLKLMEQEKVTAAKLADILEVQPSAISHILSGRNKPSFD
                     FIQKLATNFPKLNIDWLITGKGNMYKVAVQQSLFDPPSPLIPKTDNPSSKTNDKSEDL
                     QKSSKVTDVNISVSHKAVKQIVFVYSDNTFEIYSPSKSD"
     gene            38210..39259
                     /locus_tag="PK049_01900"
     CDS             38210..39259
                     /locus_tag="PK049_01900"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HOK60339.1"
                     /translation="MLYKKLIRPIFFILNPELAHKLVVATLKVAFTIPGVRFLCIKMF
                     TVSHPSLKRDVFGYHFSNPVGLAAGFDKNADYFPELACFGFSFIEVGTVTPKPQPGNP
                     KPRLFRLIPDNALVNRMGFNNKGVDYVKLNLSRKRRPNNLIIGGNIGKNTLTPNQNAI
                     DDYLTVFHGLYPHVDYIVVNVSCPNVANLRDLQDDDSLKSLLKAINAERMNYDFYKPI
                     LLKVSPDLTNEHLTATIKIAEEEGIDGYIATNTTTSRDNLQTPEAKVKTIGNGGLSGK
                     PLAKRSTEVIKLIHTLTQGAKPIIGVGGIHSPDDAIEKLKAGATLIQIYTGFIYEGPA
                     IVKRINKALINSDIV"
     gene            39275..39892
                     /gene="yedF"
                     /locus_tag="PK049_01905"
     CDS             39275..39892
                     /gene="yedF"
                     /locus_tag="PK049_01905"
                     /inference="COORDINATES: protein motif:HMM:TIGR03527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HOK60340.1"
                     /translation="MTKIIDCRGQLCPQPLINTKKALKESVKDETIQVLIDNATSCQN
                     VSRFLADNAIPFTVDEQNGLFTLTINPTGSEFNPKKEAEEYCEISFPPRGKKYIVVLT
                     SHLMGNGDNDLGKILMKGFLNTLPSLEGLPEEIICYNSAVTLAVKGSDTAQSLVKLME
                     MGVKITLCGTCVDFFSIKDKIEVGTISNMLYIAERLSSGIHTVKP"
     gene            39895..41016
                     /locus_tag="PK049_01910"
     CDS             39895..41016
                     /locus_tag="PK049_01910"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOK60341.1"
                     /translation="MANYYFDNSATSHPKPPDVAQYINEYLKLGGTYGRSAYDRVINV
                     SRIVEHTRILLARLLGTSLTEHIVFTYNATHALNIAIKGFPYRKKRVLISPLEHNAVT
                     RPVFQLQNTIGLEFDIMPYNPDGSINLEGLRQLDLKSYDLCIVNHVSNVNGVVQPIAE
                     LKALLGEIPLLVDASQSAGHIPIEVDTWNIDMLALTGHKGLMGPTGTGCLFVRTPEII
                     NPLIHGGTGSRSDSFLMPDFLPDRFEAGTPNVLGIFGLYGALSANVTNSYERKHFMDL
                     LERVEHLRSFKVFRSLDVKHQASLFSITSNKYSPSELSDQLFKRYSIETRSGLHCAPL
                     AHQTLRTFPMGTVRFSLSKYHNSTDLNYLYSALKELDHG"
     gene            41242..42282
                     /gene="selD"
                     /locus_tag="PK049_01915"
     CDS             41242..42282
                     /gene="selD"
                     /locus_tag="PK049_01915"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013816104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HOK60342.1"
                     /translation="MSTNFDLLTTVEYGGCSAKIPASVLESILLNLPKVTDPNVLVDI
                     DTHDDAGVFKINEDLALIFTTDFFPPVCSDPYEYGQIAATNSLSDVYAMGGEPFLALN
                     IAMFPSTKIPLDAYRQILEGGASVAQKANTIIIGGHTIDDYPPKYGLAVVGRVHPDKV
                     ITNAGLQPGDALILTKPLGTGVAVAAKRLGIVSEAAYSEALFWMKQLNNTAAQVMQTV
                     GVKGATDITGFGLIGHVYKMAKASRVGVKLYTSKIPLLPEVYQLVDEGCIPGAAFRNL
                     EFVGSHLTGDPNVDYNIKMLMCDAQTSGGILMGVKPERVNEALSLLKEAGLTYSAIVG
                     EVFEGDNILITS"
     gene            42394..43287
                     /locus_tag="PK049_01920"
     CDS             42394..43287
                     /locus_tag="PK049_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60343.1"
                     /translation="MIFREGEWYNFKITKVIDVPGGGKQFVLSHESGKKLLLPAQYYD
                     KYNIAENSTIQCRIDKINCTGQIFIEPAHPVYKTGGTYTFKIKHIGDTAIEEVQSIIV
                     CDIFGNEIEVLAPKGTIDDRQEEVKLKVLSVKKGVPILSAIAKWDICPDTLSNGQEIL
                     LFLSKVVVIQNEEYYLLKSKSNCIALLKVKHYKHYGFKEGIPIVARYRGTDNRGFLMI
                     DPEHPYYKVGGVYQFEIAAIEEDYSNELGSSRVAVVYDKFGMKCGLQIENASSFSVGK
                     SVSCKVIGYKKGRPLLEIVRM"
     gene            complement(43303..43632)
                     /locus_tag="PK049_01925"
     CDS             complement(43303..43632)
                     /locus_tag="PK049_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60344.1"
                     /translation="MSKKRAAVSLSNLSPELLKIFKERYPHGYQDFVFKVEKPNGDFF
                     HAVTLETEDAIYLVKVPVKIDTKIKDEDEEKEFFGSLTDDTIGTEGDEFPDDISDEEP
                     AEEIADE"
     gene            43811..44275
                     /locus_tag="PK049_01930"
     CDS             43811..44275
                     /locus_tag="PK049_01930"
                     /inference="COORDINATES: protein motif:HMM:NF025268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOK60345.1"
                     /translation="MKRLLLLNLLILLGLMANAQVNIYNPNADAKAEIAAAINKAKQE
                     NKHVFIQIGGNWCPWCVKLHNFMNADSTISEILTKNYVVVKVNYSKENKNLDVLEKLE
                     KPQRFGFPVLVILNSNGNRIHTQDSGLLESGDGYDRKKVLNFLKNWTPEALK"
     gene            complement(44356..45663)
                     /locus_tag="PK049_01935"
     CDS             complement(44356..45663)
                     /locus_tag="PK049_01935"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOK60346.1"
                     /translation="MKRLTILISMLFVAVMVTAQTSKEINFDQYAKKVEKSNAEIQDP
                     KKNTKDATWIKRGELMIDLFDAQILRAYVGMDPNTFMLVVGKPIQQEKVEVDGVQLDK
                     YVMERVNFYFNNGQLERWEVTKPVIDKPLDLALESFLKAIEINTKGKSTKKLQENLVK
                     LKGLYVNEGSNQYTLKDYKGAFESFKKVIEIGTLPILNQKDTAIYYYAGLSAQLAGLL
                     EESIPYYQKSIELGFTNDGAVYFNIFDAYKNLNKGDEGLKYLEEGFIKYPKNTNVLFS
                     LISYYIDKQEDPTKILVYIDKAIETEPNNSSLYFAKGTLYDRLGDGENALEAYKKAIE
                     IDPNFFDAYYNIGALYFNKGVKYIEEANKVPARELDKYDALMEKANAEFKNSMPYMEQ
                     AYKVNPNSKEAVEALKNIYFRFRNESQEMLDKYNEFNEKAKQM"
     gene            complement(45684..48182)
                     /gene="gyrA"
                     /locus_tag="PK049_01940"
     CDS             complement(45684..48182)
                     /gene="gyrA"
                     /locus_tag="PK049_01940"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOK60347.1"
                     /translation="MSEGERIIQINIEEEMKSSYIDYSMSVIVSRALPDVRDGLKPVH
                     RRVLFGMADLGLNAGKPFKKSARIVGEVLGKYHPHGDSSVYEAMVRMAQDWAMRYPLV
                     DGQGNFGSVDGDSPAAMRYTEARLTRIAEETLADIDKNTVDFKLNFDDTLEEPTVLPT
                     KIPLLLVNGTSGIAVGMATNMPPHNLCEIVDAICATVDNPEITIDELLKYVKGPDFPT
                     GGIIYGYQGVKEAYLSGKGRVVIRSRTEIEQTESGRSKIIVTEIPYMVNKAEMIKKIA
                     DLINDKKIEGISYINDESDRQGTRVVIILKKEAVANVVLNNLFKHTQLQSSFAINNIA
                     LVDGRPKQLNLKDLIVQFIKHRHSIILRRTQYELDEAQKRAHILEGLLIAIDHIDEVI
                     NLIRAASSPNEAKEKLMERFGLSEVQAQAIIDMRLRSLTGLEREKVKQEYEELLKQIA
                     YLKQVLEDENLQKKIIKDEMIEMKEKYGDNRKTEIVPDAEEFNPEDFYADEEVVITIS
                     HMGYIKRTPLYEYKLQSRGGVGSKGSTTRDEDFIEHIYVASMHNTMLFFTEKGKCYWL
                     KVYEIPEGNKASKGRAIQNLINIENDDTIRAYINVKTLTDPEYLNNNYIVLCTRNGII
                     KKTSLEAYSRPRQSGVMAITIKKGDQLIEACLTNGNKQIILAAREGKAIRFHEKLVRP
                     IGRTGSGVRGMNLAQGDSIIGMICVEENENKDILVVAENGYGKRSKLDDYRVTGRGGK
                     GVKTITITPKTGKLISIKAVDDNNDIMIINKSGLTIRLSAKELRVIGRTAQGVKLINL
                     RKNDSIAAIANVEASDEENIDQQETIDNDESVNE"
     gene            48371..50914
                     /locus_tag="PK049_01945"
     CDS             48371..50914
                     /locus_tag="PK049_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOK60348.1"
                     /translation="MEQKFSQKIKDVIAYSREEAIRLGNTAITPDHLFLGILRDGEGE
                     AINILLSEGLNLNDLKKSIDSRLLQPADNNSGIDESEDISIYKSTERVLKIVILEARS
                     LKSDTINTAHLLLAILKDETSYITQLLLEHNVDYETVRKHLIVEDSGDKDDFSGGGSE
                     DDEGEGGIFGNMGRSGQSSSKSSDTPVLDNFGIDLTKAAEEGRLDPIIGREREIERIA
                     QILSRRKKNNPILIGEPGVGKSAIAEGLALRIVKKKVSRVLFNKRVIALDLASIVAGT
                     KYRGQFEERMKAILNELTRSPNIILFIDEIHTIVGAGGATGSLDAANMLKPALARGEL
                     QCIGATTLDEYREHIEKDGALERRFQKVMIEPTTPEETIEILNNIKERYEEHHNVTYT
                     PDAIEACVKLTMRYISDRHLPDKAIDALDEAGSRVHISNIKVPESILKLEKLIEETKK
                     EKIKAVKNQMFELAAKYRDNEKKLIEQLEAEQARWEKELTKHRETVDAEKVAEVVAMM
                     TGVPVQRIAQAEGARLLKMADELKRSVIGQDEAIDKIVKAIQRNRAGLKDPNRPIGSF
                     IFLGPTGVGKTQLAKVLAKYLFDTTDNLIRIDMSEYMEKFSVSRLVGAPPGYIGYEEG
                     GQLTEKVRRKPYSVVLLDEVEKAHPDVFHILLQVLDEGQLTDSLGRRVDFKNTIIIMT
                     SNIGTRDLKEFGQGVGFATKAKSEAKSDYEKSIIQKALKKAFAPEFLNRIDDIVMFNS
                     LTKSDIHKIIDIELKGLFDRVAGLGYTIKISTAAKDFITEKGYDVQYGARPLKRAIQK
                     YLEDPLAEALIKSDAKPGAVFLVGYSKKNDEITIRTAKAEEEVTNADEE"
     gene            51025..51531
                     /gene="tpx"
                     /locus_tag="PK049_01950"
     CDS             51025..51531
                     /gene="tpx"
                     /locus_tag="PK049_01950"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HOK60349.1"
                     /translation="MKTVTFGGNPVTLTGEQVKVGDKALEFTALDNSLKPVKMSDFKG
                     KVKLISIFPSIDTSVCSVQNHKFNKTASEFGDKVVFIAISNDLPFALKRFCGAEGITN
                     LVTLSDHRDLEFGTNYGFLIKELRLLARGVVIIDKDDTIKYVEYVPEIAQEPNYDAAI
                     KVLKELVK"
     gene            complement(51581..52876)
                     /gene="rimO"
                     /locus_tag="PK049_01955"
     CDS             complement(51581..52876)
                     /gene="rimO"
                     /locus_tag="PK049_01955"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOK60350.1"
                     /translation="MTKKINVVTLGCSKNVVDSEVLMHQLDRGGWKIVHNSNDPSARV
                     VVINTCGFIGDAKEESVETILGFVDAKNRGLIDKVYVMGCLSQRYRNELETEIPEVDG
                     YFGVSDLPQIVAELNVKYNEAELNKRWLSTPRHYAYLKISEGCNWGCSFCAIPLIRGK
                     HVSRPIESLVNEAKELAKQGVKELIVIAQDTTYYGFDIYGERRIAQLLNSLSQIDGFE
                     WIRLHYAYPSGFPDDLIDVLKSNPKICKYIDIPLQHINTQVLKMMRRGISREQTIDFI
                     EKIRKNIPEIVIRTTFLVGHPGETPEAFNELIDFVKDYRFDRVGVFAYSEEEGTYAAQ
                     NFTDSIPDEEKTRRVEKLMEVQQRVSFELNQLRVGHTYKVVIDAIEDHYAVCRTEFDS
                     PEVDQEVLVKLNAINVKPGEFHTVRIVEAQEYDLIGELI"
     gene            complement(52883..53851)
                     /gene="ftsY"
                     /locus_tag="PK049_01960"
     CDS             complement(52883..53851)
                     /gene="ftsY"
                     /locus_tag="PK049_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019035538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOK60351.1"
                     /translation="MGILNIFNPDSKDNNNSSSLDQGLAKTKENLFKRISRAVAGKSR
                     VDDEVLDELEEGLISADVGVETTLRIIERIQKRAAAEKYLNTAELNTILKTEILSLLA
                     ENSENSILNNSGEIPKPYVIMVVGVNGVGKTTTIGKLAYMFKNQGKRVLLGAADTFRA
                     AAVEQLTIWANRVDVPIVKQQMGADPAAVAFDTLNSAKSNDTDVVIIDTAGRLHNKVN
                     LMNELSKIRRVMQKVIPDAPHEVLLVLDGSTGQNAFAQAREFTSATDVTAIALTKLDG
                     TAKGGVVLGISDQFKIPVKYIGVGEKMTDLQIFDRVAFVESLFRNV"
     gene            complement(53941..54093)
                     /locus_tag="PK049_01965"
     CDS             complement(53941..54093)
                     /locus_tag="PK049_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 family protein"
                     /protein_id="HOK60352.1"
                     /translation="MAKKVVASLQKATKDRVKCIKMVRSPKTGAYMFKEETMDKSESA
                     DFFKQK"
     gene            complement(54105..54287)
                     /gene="rpmG"
                     /locus_tag="PK049_01970"
     CDS             complement(54105..54287)
                     /gene="rpmG"
                     /locus_tag="PK049_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOK60353.1"
                     /translation="MAKKGNRVQVILECTEHKESGLPGTSRYITTKNKKNTPDRLERR
                     KYNPILKKVTLHREIK"
     gene            complement(54307..54540)
                     /gene="rpmB"
                     /locus_tag="PK049_01975"
     CDS             complement(54307..54540)
                     /gene="rpmB"
                     /locus_tag="PK049_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOK60354.1"
                     /translation="MSRVCQLTGKRVMVGNNVSHSKRRTKRRFLPNLKTKRLFLESEN
                     RWVTLRLSTSAMRTIDKVGLDNALKKFAKKGHI"
     gene            complement(54740..55984)
                     /locus_tag="PK049_01980"
     CDS             complement(54740..55984)
                     /locus_tag="PK049_01980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HOK60355.1"
                     /translation="MRASILTIGDELLIGQVIDTNSAWLADSLTELGFEVTKILSISD
                     SSQAIAEAVNELMGQSDLVISTGGLGPTSDDRTKPTLCELFGGQLMLHAPSLSLIEQI
                     FHKRGLPLTETNKNQALIPSSCTPILNYHGTAPGMIFRKGNCLLISLPGVPFEMKNMF
                     TEGVRPFIEQNFTLPAIVRVTVNTFGIPESFLSDKLKDFEIYLADRSIALAYLPSPSG
                     IRLRLTATGENKGRLSNLLDMAVNELKSLIGENIFGFNDETLPSVVSKRLREKNATLA
                     VAESCTGGYISHLITQLPGSSDIYKGGIVAYSNEIKQNVLGVSHDLLISHGAVSQPVV
                     EAMLDGTLRVFQSEYAIAVSGIAGPTGATAGKPVGTTWIGVASRETRLVELYTFGNLR
                     EVNIQRAAYTALHQLLKLMDKD"
     gene            complement(56034..56107)
                     /locus_tag="PK049_01985"
     tRNA            complement(56034..56107)
                     /locus_tag="PK049_01985"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56071..56073),aa:Thr,seq:cgt)
     gene            complement(56247..57230)
                     /locus_tag="PK049_01990"
     CDS             complement(56247..57230)
                     /locus_tag="PK049_01990"
                     /inference="COORDINATES: protein motif:HMM:NF040069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6340 family protein"
                     /protein_id="HOK60356.1"
                     /translation="MWIKKFKWLVLLFSLTSCISYRYIDIEFYDSPQKSIVVPGENLL
                     VVENLHLRNTDTPIRELDWALDSVASNEATDALISLLKTSPWYSGITLFKHIYYRNDS
                     SRVVLPLSWQVIDSLSNVYNSKMVVSLEYLKVRPYYDTYPLWRGDFKEYYGFIQVSSY
                     TYWRIYNVETRKINLGQLLTDTLLWEKNDWIEVTPGNQLPGIFEACAFAGADAGEQFA
                     QLIAPQWQKDSRAIYVPSTNKEMSRAYQLAMHGNWLDAAAVWQRLSSSTSKELAAQAA
                     FNMALANEMLGKFQLSIEWLDFAKSKYPHLKFLKEYKVILQHRIENNKPQK"
     gene            complement(57336..58082)
                     /gene="fabG"
                     /locus_tag="PK049_01995"
     CDS             complement(57336..58082)
                     /gene="fabG"
                     /locus_tag="PK049_01995"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOK60357.1"
                     /translation="MKLLEGKTGLVTGAARGIGRAIAIAFAQHGANVAFTDIAYDDNM
                     KSLEQELTALGVKAKGYASDASDYDNSQKIVDEIAKDFGQIDILVNNAGITRDTLLMR
                     MTEEQWDLVIKVNLKSVFNLTKAVQKYMLKQKNGSIINMSSVVGVSGNAGQANYAASK
                     AGIIGFTKAVARELGSRNIRCNAIAPGFIITEMTHKLPDEVREDWIKKIPLQRGGTPD
                     DVANVCVFLGSDLSSYVSGQVINVCGAMNT"
     gene            58252..60579
                     /locus_tag="PK049_02000"
     CDS             58252..60579
                     /locus_tag="PK049_02000"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOK60358.1"
                     /translation="MKVKIHIRVKLIIAVLTVTAVVFWASIAFLNKRVDRYTRTNIYE
                     YIDATSKTYTTQIKGEFDKQFALARTITYSFYDLEQRNPEDVKQNTLRLLKGIAENTP
                     DLLSVWASWELWALDRKWDKPYGRDRYTYLRENNELKQIFERLNETGDNPSSLYYKLK
                     TEKKEALVDPYWYIYAGSQKRVLETSVCVPLVVNNRFAALFGFDIELSVYQEMIKNLK
                     PYEGSYAILISNNGTIVAHPDSTLLGALADTVLTNGKQIINSVITNGKYSETVLEGKS
                     KYYLTFNSFTAGQAAERWSLGIFVPEKVINEQISTITSQVRIVMVIGLIVLAIVLWII
                     AYSITSPLVRATKVLGELSLGKIDPAKKIEIKTGDEIEDIASSINILIDSLFKTSQFA
                     REIGKGNLQAKYSKLSDDDVLGDALLEMRKSLEYAKKIDEERKAEENKNRWYNEGMAK
                     FAEILRHNNSNLNDFAYETIRNLTKYVDATIGAVFLINNDNPNDIFLELMATYAYERR
                     KYEEKVIRMGEGLVGRCAQEAETIYMTEIPNGYIKIASGLGQDEPTELLLVPMKINDE
                     VHGVIEIASFEPIEDYKIKFIEKIGESFAATISSVKINIRTAKLLEESRIKSEELASQ
                     EEEMRQNMEELQATQEESARKSAEMESLINALHSSSYVIEYDLNGNIITVNEAYLKLT
                     GQTEKDIVGTHHADNLEMTSEQKNAYQKFWQDLRNGMIKKETSKVKIGGKTYTFIETY
                     SPILDENRRVVKILKIAHNITDFIDENPEGKTKKK"
BASE COUNT        17590 a        12218 c        12486 g        18010 t
ORIGIN      
        1 acttttaacc tttaaccttt aacctttatc ctttaccctt tacccattat cctttaccct
       61 ttatccttta tcctttatcc tttatccttt atcctttatc ctttatcctt acttcccctc
      121 aatctgttta atagtccatt caagggcttc ctccagcatt tcggttggtg ggtcggttat
      181 gaagccatta tcgtgtaacg actttaccat ggaacgagag tattcaatat cctttttggc
      241 aatgctgtac gcctcatccc agctcattgg cctacgggca acgccatctt taacagcctg
      301 tacagccaca tcggctgctt caacggggaa tacgtcggct tcatccatgg ttgggacaat
      361 attatcggga ttaattccac gtttctcggc gtagtttgcc agtgagtgag cagcggcaat
      421 ggccatgtta tcggttattt ttgaagctct aaccagcaag gctcctttaa gtattccagg
      481 gaatcctaac gagttattta cctggtttgg gaaatcgcca cggcccgtag ccacaatgta
      541 tgcccctgct gcttttgctg catatgggta aatttcaggt acggggttgg cgcaggcaaa
      601 aacaatggct ttatcggcca taagcctaat ccaatcctgc ttaatggtgt cagggcctgg
      661 ggtggaaagc gatattagca catcggcgcc tttcatggcc tcctccatgg tgttaatgca
      721 atcgggattg gtttgctcgc aaatctccca tttgcggtaa aaacggctat cggctttaat
      781 gtcgtcacgt ttgcgatgaa gtgcaccatg tgagtcgaac ataatcattt ttttaggatc
      841 accaccatca gctatcatca ggcgggcaat ggtggtattg gaggcaccag ctccaaaaaa
      901 tacaatgcga acatctttta gctttttatt taccagcttc aatgcattga gtaacccagc
      961 aagggtaacg catgcagttc cctgggcatc atcgtgccaa accggaatca cacattcctc
     1021 cctcagtaca tcgagaactc tatagcagtt aggttgtgag atatcctcaa ggtttacagc
     1081 gccaaagctg tgctggcaca tctttacaaa atcgattatc ttttctgggt cgtttttgcc
     1141 atctttcccc ttgctgtcaa tacaaagagc aactgcatcc acgccaccaa ggtacttcat
     1201 caggaatgct ttcccttcca tcactcccag accacccgga ggggtgcaat cgccatcgcc
     1261 aagtacacgg gtcgagtcgc taacaacggc aactaaattg cccctgttcg aaagttcaaa
     1321 tgacgcatca ttattatcgc gtatagttgt tgatattttg gagactcctg gggtgtacca
     1381 aacattgaac cagttaaagc caagcacagg tgctttgggt gctgtttgga ttttacctcc
     1441 gtaatgctta tgggccttct cgctgaggta ttttaagaat aaagttttac cctttgcgcg
     1501 ttgctcctca gtccaatttt ctgggaacat ttcgcctagg cgttctaatt tgatgtttac
     1561 tttactcata tgtttaattt ttaattgatt agatttcgat ttgctgatat aatttttacc
     1621 caagatacaa aattcaaact aaacaatcta gttttttcaa tatgttatac agtatgtttt
     1681 agttgctttt tttgacaaaa taatgtaggt ttgaggtcgc aatacgtgtt gtatttttac
     1741 gattaacgat tcaacaaaat ccatgatatg agtgtattag tcaataaaag ttcacgtgta
     1801 attgttcagg gctttacagg aggtgaaggc acttttcatg ctacccagat gattgagtat
     1861 ggaaccaacg ttgtgggtgg agtaactccg ggcaagggag gacaaacaca tctcgatagg
     1921 cctgtgttca atactgtttc cgaagcagtg aaagaaaccg gtgccgatgt atcggtaata
     1981 tttgtacccc cgacttttgc tgccgatggc attatggaag ctgccgctgc aggaataaag
     2041 gtaatcgttt gtattactga aggtattccc acaaacgata tggttaaggt taagcacttc
     2101 cttgctgatt accccgagac ccgcttagtt ggacctaact gtcctggagt aataactccc
     2161 gaagaggcta aggttgggat aatgccaggg tttatccata aaaagggaca tgttggcgtt
     2221 gtatcgcgtt ctggcacatt aacctacgag gccgttgatc agcttaccaa gttggggttg
     2281 ggtcaaagca cttgcattgg cattggtggc gatccggtaa ttggaacaac cacgctcgaa
     2341 gctgttaagc tactaatggc cgatcccgat acacatggta ttgtgatgat aggtgaaata
     2401 ggcggtagca tggaaaccga tgctgctcga tggattaagg agcatggaac taagcctgtt
     2461 gtgggcttta ttgctggaca aactgctccc aaaggtcgcc gtatgggtca tgctggggct
     2521 attattggtg gtgccgacga taccgctgct gctaaaatga agatcatgcg tgagtgtggc
     2581 attcatgtag tggaatcgcc tgctaacatt ggtcaaacca tgaaggatgc gctgaaataa
     2641 tattctacca aaaacaataa aggccggcaa atattgagcc ggcctttttt attcccgtat
     2701 tggttaatcc tttactttta tgtctattgc ctcagttttc ccttttatta gcannnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngatgtct tggcaaaaat acaaaaaaga
     2881 tgtttgtaag ctacttctct gaatctaatt cgtcgccggt aaaggcgggg taaacatctg
     2941 tcatgtaacc catgtataat ccaacacgtg tcatccatct aagttgtccc acaacccaac
     3001 tgtgaaactc tctggggtat ttacctgtaa ttagtactat caaccatgcc aaaatgataa
     3061 taacgcctac aaatatttgc ctaaagaaca aaataaagag gtgtggaatg taaacataaa
     3121 gccaaccaaa aaagaaacga accagcatta atgcacggct taccctttca gggtattcaa
     3181 cctctaatga ggtatactca tcggttccat taatgccaaa cgaagggtat ccatcggata
     3241 tgtttaatac cctggcgttc acacgtaatt tccatcggaa tagtcctacc tgaaagtcaa
     3301 ataaactttt tgggtattta ccagtaaata gtattgccca aaatgctaca atctgcagga
     3361 ttagagccca aataaataca aaaaatagga ggaaaccgtg tggcagtaga atgtacagcc
     3421 agccgaaaaa tgttctcagg agcaattcta acctagagta tcgttcctga tgttgaactt
     3481 ttagtttcat tttatttaag ttttaggtta gggagatttt gaactcaaac aagttacaga
     3541 tttttttatt tcaaatagta gaaatcattt aaaattattc ctaaattagc aaaggcctgt
     3601 aattacgggc atttcctatt cgcaaacgtg tgaaagctga taaatcaatt tcaaataaac
     3661 caatagcaat atgagacaaa agaatctatt tagcttgaga aacattttgt ttgcagccct
     3721 aattgctgca acaatggtat ttgcctcgtg taagcagcaa cctaaggagg ctgagccatt
     3781 ccgcgtagag catgccaatt gggtttacaa tgctaccatt tacgaggtaa atgttaggca
     3841 atacacaccg gaaggaacat tcaaagcctt tgagcaacac ctgcctcgcc taaaagattt
     3901 aggggttgat atactttggt tcatgccaat tcatcccatt ggagttgaga accgtaaagg
     3961 aacgctagga agctactact ccgtaaagga ttactacggt attaaccccg agttcggaac
     4021 actcgatgac tttaagtcag ttgttgccaa ggctcatgag ttgggtatga aggtgataat
     4081 tgacattgtg gcaaaccaca cctcgcacga tgctgttctc ttaactgaac atcccgattg
     4141 gtacgttcgt gattcctcgg gtagggttgt ttcaccattc gattggaccg acgtggctaa
     4201 gttggactac tcaaaacctg aactacgccg ttacatgatt gacatgctta agtattggat
     4261 taaggatatt gacctcgatg ggtttaggtg cgatgtggcc gctgaggttc caaccgattt
     4321 ctggaacgag gctcgtgctg agctcaacca gcttggcaaa cccattttca tgcttgccga
     4381 ggccgaaacc cctgaactcc aaaaatatgc gtttgatgct gattatgcct gggagttcca
     4441 tcatattatg aatggtattg ctcagggcaa gaaatctgtt gttgagctgg agcaatacct
     4501 gctcaaaaaa gcagccactt atcctaagaa taccattaaa atgaatttta tcaccaacca
     4561 cgatgagaat tcatggaatg gaactgagtt tgagcgtatg ggcgatgcgg ttaaaaccat
     4621 ggctgcactc acatttgtta ttgatgggat gcctcttatt tataccggcc aggaggttgg
     4681 tctaaaccat aggctacagt tttttgaaaa ggaccaggtt gactggaccg atagtaaagg
     4741 tttgactgat ttttacgaaa aactcactgc gttaaagaag tccgcaaagg ttcttaatgc
     4801 cgcagagcag ggagccgaca ttttccgaat aacaaccact gccgacagct caattttcgc
     4861 atttatacgc gaaaatgaaa ctcaaaagct atttgctgtt ttcaacctat cggccaagga
     4921 acagaccttt aactttactg gcgatgcgca tttgaatacc agctatgttg agtatttcag
     4981 caatagctct aaagagttca caaaggatga gagagtaaca ctaaaaccat gggagttctc
     5041 aatctactta aacaataagt aaagttttca agagtaaaaa agaggctgtc ctcatagggc
     5101 agcctctttt gattttatag aatttaaaag gttttaccta ttacttgatg atgctaactt
     5161 attcttaaga tactattttt atttagattg atttttaaca actggttagt tgtttaacaa
     5221 atcactttaa ctttctaatt ttgccataag attaaaaaca aaaaatactt aagcattatg
     5281 tattacaata tccctgttgt tggaaaggtt cactggatag gggtgaacga tcgacgtaag
     5341 aggctttttg agaacatctg gcctttggat aggggcgttt cctacaattc atacctgatt
     5401 gttgatgaaa aaaccgcact gatcgatacc atagaggatc gggcggccgg taattacatt
     5461 gagcgtataa aggcattact taatggacgt acgcttgact acctgattat taaccatatg
     5521 gagcccgacc attccgggga gattaaagac atttttgagc aatttaaagg ggttaagctg
     5581 gttggaaatg ccaaaacttt taagatggtt gaagcttact ggggtattaa tgaaaaccta
     5641 atacaggtag atgatggcga tacccttgac ctcggtttcc acaagctgaa atttgtgatg
     5701 acaccatggg tgcattggcc cgaaaccatg atgaccttcg atcaaaccga acaaatcctt
     5761 ttctccggcg atgcctttgg cagctttggc gcgttagatg gcggtatttt tgataatgaa
     5821 atcaactttg atttctttga ggatgagatg cgccgctatt attcaaatat agtagggaaa
     5881 tacagcaata tggtgcaaaa agcttttgca aagcttaagg atgtacctgt aaaggtgatt
     5941 gcacctactc atggccccgt ttggcggtca aaccccaata aagtaattga actgtatgac
     6001 cggtggagcc gttacgaggc tgaggaaggc gttgttatca tttttgcatc aatgtacggt
     6061 aatacggaag ctattgccga ttacataggc cgtaaagtgg cagagaatgg tattaagaac
     6121 attcgcatac acgatatttc tcgcacccac attagccatt tgattaacga aatttggaaa
     6181 tatcgtggac ttgttttggg gtcatgtgca tataactcgg aaatgtttcc tcttatggag
     6241 cagcttaccc gtgagctaac acatatgggt ataaaaaata aaaagttggc tctttttggc
     6301 agctacagct ggaatggcgg tggagtgaaa aacctactta agtttgctga ggaagccaaa
     6361 cttgagctag ttgctgaacc tgccgagata tatggcaaac ctaatgccga aaagcttaag
     6421 cagttcgatt tgttagctca gaagattgca gagagcgtta agtaggaaaa tacctgatga
     6481 aaaaatgaaa aagagacgtt taaacgtctc ttttttattt aaaaaccatt gcaagtaata
     6541 agctttcctg aactggaagg cattacttaa agttgtaatg tttccgaata ggctttttaa
     6601 ccgtccaaac acattttaac cctttcttaa aggtaacaaa gtcgcctgtc ctaacagtgt
     6661 aggtaccctc gtcggttttt acatctacct caccatcaag aatcaggcat tcctcatcgg
     6721 tatcgtaata ccaatcaaaa gtagatattt ccttagtcca aataggccaa ttctttatgc
     6781 ccattttgct tatttgttca tccgttagct tttcaattat caccttgctc atggttgctt
     6841 aacttggttt acatgcaaaa atagaaataa atgggctaag ttgaaaattg ttcatatctt
     6901 tgtaaaagca ctaaaaacac taatggtatg gagtttaaca ttcctcttgg cattgaattt
     6961 actgcaaccg aaatggttac aaaggataac actgcatcaa aatacggctc aggccttgtt
     7021 gatgtttttg ccacacccgc tatggtagca ctcatggaga aggctgctct taatgctgta
     7081 ttacctcatt tgccttatgg gttcaatacc gttggtactg aaatttgtgt taaacacacc
     7141 aaggctacac ctttaggctg ggaagttcac agcaaggcca cccttaaaga ggtagatggc
     7201 aaaaaacttg tttttgatgt ggtggcatgg gatagtgaag gagaaattgg caggggtacc
     7261 catacccgct acattatcga cagtaaacgt tttatggaaa agttttcatc caaatagtct
     7321 actttgatga attttgatac ctatcagttc ccaaaatacc acaggcataa gctaaaggtt
     7381 cattcgtacg aggttgattt taaccaaagg ttaacagtta cggccctgtt taattacttg
     7441 caggagattg cctgggagca tgcccattta ctgggttatg gctgggagca tctgttacag
     7501 aagggttggt tttgggctct gtcgcgtgtt gaagctgaaa tataccggtt acctgcatgg
     7561 accgatgagg tagttctgct aacctggcct aggggagttg atggaatttt tgccttacgc
     7621 gattttgagg tatatgacca tttaggaaat acaataattg ctgcttcatc gagctggttg
     7681 gttcttaaca ttcaaacccg tcggccagta agggctgagt ggtactccga tttcgattat
     7741 gcaactagaa gttcattagg gcgcaatgca accaaactgg ttgaacaaag cgttagccct
     7801 gaattttcgg aaaaatttga tgttcgcatt ggcgatatag atatgaatca gcacgttaat
     7861 aatgttaggt atatcgattg ggcctacaat acctttagca ttgagcattt taaaagctat
     7921 tttcccaaaa gggtagtggt taactttaat gctgaaggta aagcaaacga tagcataatt
     7981 gcatcacgtt ttcaaaattc gcaagatgag agcattgttg aaattgcgaa cgccagcaac
     8041 ggaaaaaact tgtgccggtt gatgtttgaa tggggaaaaa tttaataaat ttaaaattaa
     8101 ttttctttcc caatcattat gaaagtttta ataaaataga acccaaaaaa taacactatg
     8161 tatagccagc aggatattgt gcagatgcag cgaaagggaa taactcccga aatggttgag
     8221 cagcaaattc aatcatttaa aaagggcttc ccttacatga agttggttaa acccgctacc
     8281 gtgggcaacg ggataattcg gttggatgga gaggaacttg atagcctcat tgaaatgtac
     8341 cgcgattttg agggtagtag ggttaagttt gttcctgcat cgggtgcggc aacccgtatg
     8401 tttaaacatc tctatgagtt tcagtccgat tatccggtaa aagggttgga gtgttttaac
     8461 gataaaggtt ttaataccgt tttcaccttt ttcgagagaa taaaagagtt cccatttttc
     8521 gacaagcttt acaatgaact ctggatgcag ggttataaga tggatgaact tattgatagt
     8581 aaggattata tgcccatagt aaatactctg ctaggttccg aggggctaaa ttacggaaat
     8641 ctgccaaaag cgcttatcct attccacaag tatggcgata ttgctcgtac tgccatggaa
     8701 gagcatctgg ttgagggggc acagtatgct cgcgaccagc atggtaatgt aaatattcac
     8761 cttacggtgt cgcccgaaca cattgctggc tttgagcagc tgattgaacg cgtaaagtca
     8821 tactacgaaa accactatga agtaaccctt aacattacct actcggttca aaagccatca
     8881 accgacacca ttgcggttga tatgaacaat aatcctttcc gtaacgatga tggcactcta
     8941 ctttttcgcc ccggtgggca tggtgcatta attgagaatc tcaacgattt aggccacgaa
     9001 ttggtgttca ttaagaatat cgacaacgtt gctccagacc atcttaaacc gctaaccgtt
     9061 cggtataaaa aagcacttgc gggaatgctg cttagctacc gaggtattat ttataaatac
     9121 ctgcaagagc tcgacagcca aaacctttcg gttgacgaac ttttggcaat tcttgacttt
     9181 acccgcgatg agctctgtgt tataccaccg gcatcgttaa atacctcaga ccctgcggag
     9241 ctgaacaagt atctgtttag cgtgcttaat aggccaatta gggtttgcgg tatggttaaa
     9301 aatgagggag agcccggtgg tggcccattc tgggcagtta atcccgacgg cacaacatcg
     9361 cttcagattg ttgaaagttc acagatagat atgaacgacc ctacttctaa agctatcctg
     9421 aatgcatcaa cccatttcaa ccctgttgat atagtatgtt cattcattga ttttaaaggg
     9481 aataagttta acctgcttaa ctaccgcgat ccgcaaaccg gctttatttc agtaaagagc
     9541 aaggatggta aacagctcaa ggcgttagag cttccggggc tttggaatgg ggccatgagc
     9601 aactggaata ccatttttgt tgaagtaccc ttggcaacat tctcgccagt taaaactgta
     9661 aacgaccttt tgcgggagga acatcaaccc tgcaggtaag caagctatat tagattatca
     9721 ggatactcta tccttgtaag gtataacccc tcggccggag cagaggttcc ggccaatttt
     9781 ctatcacgct tctcaattac ttgggcaaac tcttctggcg ttatttttcc tcttcccaca
     9841 tcaattagag tgcctacaat ggcacgtacc atgttgcgta aaaaccggtt agcctcaatg
     9901 gtaaatatga gcaggttacc actctcgttc cattccgctt tatatatctg gcagttattg
     9961 gtttttgttt cggagccaac cttgctaaag ctggtaaaat cggtatagtt cattaactgt
    10021 tgtgctgcca gctgcatatt atcaatgttt agctgggcat cgtaaaacca cgataggcca
    10081 atccagaagg gatctttgtt acggcaaatc acatactcat aaccccttaa ggtggcatcg
    10141 aaacgggcat tcgcgctcgg gtttaccttt ttaatttgat gaatggcaat atcgttgggt
    10201 aaaattctgt ttaagctgtg caaaaactta ccgtctgatt ccaaattgct ggctacagaa
    10261 tcgaaatgcg ccacatagaa cctggcatga acgcccgtat cggttctacc tgcacctatg
    10321 gtttgaatgt tttcctttag cagtatactc aaagcattgt ttactaagtt ctggacactg
    10381 gtggcgtttt cctgaatctg ccagccatga tagtttgtgc ccctgtacga taggtaaatg
    10441 aaatatctat ttattgccat tatacttgct tttgatcttc ctttatgaca tcgtttgctc
    10501 actatgtatt taaaagaaac atgaaaaaaa aaaggtcccc tgattcctgt tcaataatcc
    10561 attttgcatt aaattacaca acaaatatac tcgtatataa agctattttg aaatatttcc
    10621 gagattcgtc catagaattt ttctattgac gaatttcctc taaaaatgtt tctgtatcca
    10681 catttttatt agcccggttg aacttgttaa ttcatggtcg tttggtttag ctgttcactt
    10741 tttactattc ctctaacnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnctc
    10861 catcatattc tctctattcc gactcattcc gttcattccg aatcattcct caaaaattta
    10921 attcatggta cttggattta tttaaaaatc aattataaaa gtgtctgaaa gcctttaact
    10981 acctttatta tagctaaaat ttcatttaac gaaaattaac atcgcaggca tctatttatt
    11041 atcatatttg caattgaggt ttcatataag gttaaacaaa ttttaaaaag atatttttat
    11101 gagtgttcaa tcgacccttg acattaagga gctaaatgag aggataagac ttgaaagttc
    11161 ctttgttgac attatcaaca ttgaaatggg caaggtaatt gttggccaaa aacaccttgt
    11221 tgagtcgtta ctggttggat tacttgccga tggacacatt ctacttgaag gtgttcctgg
    11281 cttagccaaa acgcttgcca taaatacttt ggcaaacatc atcgatgtaa agtttaaccg
    11341 tattcagttc acccccgatt tgcttcctgc cgaccttatt ggaacactta tttacagccc
    11401 aaaatccgaa caatttaccg ttaaaaaagg gccaattttt gcaaacttta ttcttgccga
    11461 tgaaattaac cgtgctccag ctaaggttca gagcgctttg cttgaggcca tgcaggaacg
    11521 tcaggtaacc attggcgacg aaacctacaa gctcgaagaa cctttcctgg ttttggcaac
    11581 tcagaatcca attgaacagg aaggaaccta tccgcttcct gaggctcagg tcgaccgttt
    11641 catgcttaag gtcaaaattg gttatccaaa gctggaggag gaacgcctta tcatgcgtga
    11701 aaatctggcc atgcagttcc caaaaccaga aaaagtccta aaacccaccg atattgtgaa
    11761 ggcgcgtcag gttgttcgtg aggtttacat ggatgagaaa attgaaaagt atattctgga
    11821 tatagtgttc gcaactcgct atccagcaca gtataacctg cagcgatttg aacctatgat
    11881 ttcctatgga gcctcacctc gtgcatccat tagccttggg ttggcttcaa aggcttacgc
    11941 ctttattaaa cgtcgtggat acgttattcc tgaggatgtt cgcgctgtat gtttcgatgt
    12001 tttgcgtcat cgtattggat taacctacga ggccgaggcc gaaaacatta catcggagga
    12061 tataattact gaaatactta acacgataga ggttccatag caaccatgaa gccattggct
    12121 atcatattcc tttcagtagc agtcgctttc ttgagcctat cagttagcgc tcagaattac
    12181 attagcttgc acaaggatga aatagccaag cggatgccgc aggagaataa aggtttctac
    12241 tttgaaaagg aggtgaatgt tgacgaccga ggctttttga agtatgttaa caccctcgat
    12301 gagcagacta tacttttcat gattgatact gatggctttt gcactgcggt atctagaatg
    12361 tataacacgt ggctttacga ccaggttgtg aatgaactga atagcaaata tcaacaggta
    12421 tcaaccaata agtggcttga agttaaggag ggcaagagtt acgatttaac cctgataaaa
    12481 gggaaatggt ttcttaccct taccatcagg cctcactcta aataggtata gccgtggaaa
    12541 cagcagaact tctgaaaaaa gttcgacgta ttgagattaa aacaagggga ttaacccgcc
    12601 aaatatttgc cggtcaatac catagcgctt ttaaggggcg cggtatggcc tttagcgagg
    12661 ttcgcgagta tagggtaggt gacgatattc gcagtatcga ctggaacgtt actgctcgtt
    12721 acaaccaacc ctacgttaag gtttttgagg aggagcgtga acttactgtt atgcttctta
    12781 tcgatgttag cggttcgcag aactttggct ccacccaaca ctttaaaagg catgtaatag
    12841 ctgaggttgc tgctgttctt gccttttcgg ccattcaaaa taacgataaa attggggtta
    12901 ttttattttc cgataaggtg gaaaaattta ttccacccca gaagggtagc aaacacatac
    12961 tcagaataat aagcgaattg ctcaattttg agccgtcggg caaggaaaca aatattgctg
    13021 aggcgcttag gttcttgaca aatgccataa aaaagcgcag cactgctttt gtgctgagcg
    13081 atttcatcga ttttaatgaa cagaataacc aaccaaggtt taccgatgcc attagcattg
    13141 ctaacaataa gcacgatgtt gtggctatac ggatttacga tgatcgcgaa actcaacttc
    13201 catccgttgg ccttgtccgt ttcaccgatg ccgaaacagg taatgacatt tggattgaca
    13261 cagcaaacaa actggtgcag aaccattaca gccaatggtg gaatatcact actgaaaatc
    13321 tgaaacagac ttttaccaaa gctaaggtgg attgggtatc ggtgcgaacc gacgaggatt
    13381 acgtaaaacc cctaatgttg cttttcaaaa ggaggtcgaa atgaagttaa taaggttcgt
    13441 ttttatagtt tttttgctac taacccttaa cgtcgatgct cagattgagc aacttgaaat
    13501 aaaggctgta ctaaaagccg atacaatact gataggtgat agggtggagt ttgcgattat
    13561 agctaatggg aaaggaaatt tttcggtatc cttaccagta ctagccgata ctttacccgg
    13621 ggggatatac attattggta aacctattat cgattctact attaaagata atacccgtag
    13681 ctacagcttt aaacttcctg ttacggcatt ttctcctgga aatgttagtg tttctaagtt
    13741 cccggttttg gtcaagcatg gtggtagggt tgatactacg tggataacca ccgatagctt
    13801 atttgtaaag cttgttccac gcgacaccac ccttaaggat atcaaggata ttaagccccc
    13861 aataaaggaa ccaatccgat tttcggaggt ggctccttgg gccggattag gcttactctt
    13921 tgctgcaatt attgttctgc ttgttatgta cttgcgtagt cgcaagcaaa ataagccatt
    13981 cctcaatatc tttaagccaa aagaacccgc tcatgttgtt gctcttaggg agttgatgct
    14041 gctcgaggag aatagggatt gggcttcgga taatcccaaa ctgttctata ccaagctgat
    14101 cgatattctt cggaattacc tggaaggacg ttttcagatt aatgctcctg agcaaactac
    14161 aagcgagatt ctttctgaaa tagataagct tgacttcgat ttttctccct tcattgaaac
    14221 ccttcgcgag ttgctcttta cttccgacct ggtaaaattt gctaagcatg taactaccat
    14281 cgatgagaac cgtagctttt tgagctttgc aatcaatttt gtaaacagca ctaaacctat
    14341 tgaaagttca aacgatggtg aaaacacaac cgatgagata aagagtgccg ataataccga
    14401 tttattaacc agtgattcaa actaaccggt tatgctttta gtagtttatg caaacccaaa
    14461 gcttttatac ctgttactgc taattccagt tttagtgggg tggtatattt acaggttaca
    14521 taaatcgagg gctgcgctta taatttcaac agcaaaacac ctagataata ctggcaagtc
    14581 agtaaaggta tatttgcggc attttccatt tgcattaaga atgttagcat tgacattggc
    14641 aattattgca ttggccaggc cacaatcaac taatatccaa cggaatgtta ccaccgaagg
    14701 tattgatatt gtgttatcac tcgatgtatc gggtagtatg ctcgcccgtg attttaagcc
    14761 cgataggctt gaggctgcaa agagtttggg tattaatttt ataagtggcc gagctaacga
    14821 ccgcattgga ctcgtaattt ttgcaggtga aagttttacc ctttgcccat taactaccga
    14881 cagagcaact ctgattaatc agtttaaagc tgtagatatt ggtgtacttg aggatggcac
    14941 agctataggc tctggtttag ccaccgcaat ttctcgcata aaggatagcg atgctaaaag
    15001 caaggtgata atcctcctca ccgatggggt taataaccgt ggcgagatag ccccaatgac
    15061 ggcagccgaa atggcaaaaa cctttggtat tagggtttac accattgggg tagggacata
    15121 tggaacggct ccttatcccg ttcaaacccc ctttggaacc cgttaccagg acgtgcaggt
    15181 tgaaattgat gaagatttgc tgaaaggtat tgcccaaatg actggcggcg aatacttcag
    15241 agcaacaaac aataaggcac ttgaacaggt ttaccaaaag attgaccagc tggaaaaatc
    15301 aaaaatagag gtcaatgagt atgctaagcg tgaggaaaac tatcgtggct ggttattggc
    15361 agcctttatt ttgcttttac tggaatttgt tctacgtaga acttatttat taggaattta
    15421 attttaggat tgccatgttt cgtttttcta acccagaata tctttatctc ttactgctta
    15481 taccagtaat tattggatta ttctggtatt ccgttcgtaa gcaaaagcag tggattaata
    15541 gttttggcat atacaaaacc atcctaaaat taatgccagg gcattcgttc aatcgctact
    15601 ggattaagtt tatcattgcc actgctgcat acatttttat cattattgca cttgctggcc
    15661 cacaggtagg tgccaaactt actgaggtaa aacgcaaggg tatggagatg attattgccc
    15721 ttgatgtttc caacagcatg ttagcacagg atatcaaacc aaatcgcatt gagcgcgcca
    15781 agcaagccat ttcacaactt atcgataggt ttaacaacga ccgtattggg ttgatagttt
    15841 ttgctggcga tgcctacatt cagctacccg ttactaacga ctatgcatcg gctaaactct
    15901 ttttgtcttc aataaatcca ggaatagttc ctaagcaggg aactgccata ggcagggcta
    15961 ttgagctggc tgtaaattcc ttttcgccca atgcaaatac cggaaaggtg atagctttaa
    16021 tttccgatgg cgaaaaccat accgacgatc ctattgaagc tgcagcaatg gctgccgaaa
    16081 acggcatttc tatctacacc attggcattg gttcccctga aggggcgcct attccattag
    16141 ggaataatca ggagttcctt aaagatgccg atggaaaggt agttattacc cgactggatg
    16201 aagaaaccct aacaaaaatt gctgttaccg ctaacgggaa gtatgttagg gcttcaaata
    16261 cccagtttgg tttattgcca atttatgaat cacttaaaaa ggttgaccgt aaagcaatta
    16321 aggataaggt ttactccgag tacaatgagc agtatcagta tatattggct atcgccattg
    16381 cgcttttatt tgctgaatta atcatccttg accgtaaaac ttcgctttta tcaaaggtaa
    16441 acctttttgg taccgataaa acaagggagg aactatgagg aaagatattg tacttatcat
    16501 tcttttagct ggactcacgg ttaacacttt tgctcaaagt gagcataagc taatacgacg
    16561 tggtaatagg gcatatagta aaaacgatta tttggagtcc gaagtgcaat accgtgaagc
    16621 acttgagagg aataagcatt ccttcaaggc aaacttcaac cttggcgatg ccctctacaa
    16681 gcaaaacaaa ttctctgatt ccgattcaat ctttaatggt ataaacacat ccgctctttc
    16741 cgatgctgat aagtcgatga tttattacaa taaagggaat tcgcttttca agcaaaataa
    16801 gtttaaggaa agtgccgatg cctataaaat ggccctcaag tataatccta acgatattga
    16861 cgctaagtat aacctttccg aggcgcttag aatgatgcag aaccagcaga accagaacaa
    16921 caatcagcag caaaacaata gcaacgatag taaggataac aaggatcaga aagacgataa
    16981 aaagcaagac aaaaaggaag ataaaaagga taataacaac aataaacagg acaatcaaca
    17041 gaataaccag aataaaaata accaacagca acaacaacca aagatttcga aagaggatgc
    17101 agagcgaatg ctacaggcat tacagcaacg cgaaaaggac attcaggata aactaaataa
    17161 aaaacaagcc aagccggttt cgggggcttc aatcaaaaac tggtaaaccc tatgaaacga
    17221 tacctaagcg gaatactatt gcttttcatc agtggttact tacagtccca ggaagtaacc
    17281 ttacaggcat acgcaccaaa tctggttgaa gtgggtgagc agttcagctt atcgtttact
    17341 ttaaatgcta aaccatcaga gtttaaggct ccctctatag ttgatttcga tattttagct
    17401 ggccctagca catccagcag cacaagtatt gaggtgatta acggtaaagt tacccaaagt
    17461 caatcctata cctacacnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncct
    17581 taaagctaac gatgctttta cctacaggct taaaatatca gggaaaggaa accttaagct
    17641 aattgggaac ccaaaactta atttcccctc aacatttgaa gtttttgacc caaaaatttc
    17701 cgagaacatt tcagttgatg agggtaatgc aatgggtagc aaaacttacg agtatgtttg
    17761 cataccaaga gctggcggta atttcagcat agaaccggct gtgttttctt attttgaccc
    17821 tgataaggaa tcttatatta cccttaaaac atcagctatc tctgttgccg taagtgccga
    17881 ttcgtcaagc actggcaatg ttatggttgc aagttttggc aaggaagata tcaagtttat
    17941 cgggaaagat attcgtttta ttaaaacatc aagctctgct ttcaaatcaa aatctgcatt
    18001 ttgggttacg tcgggttatt atcaacttct atacatatta ctaattgcat tattcattac
    18061 attttcgtat ctattccgtc gctatcgcca gcgaatgcaa gatgtagcct ttgttaaaac
    18121 ccggagggca agcaaggtgg cacagaaacg tctcaaaatg gcgtcgcaac tacttgagca
    18181 gaacaataat gcccagttct ttgaggagat ccataaagct atttgggggt atgtgtccga
    18241 taaactcaat attcctttgg catcgctaag cctcgataat gcatcggaga agctaaccca
    18301 acagggtgtt gatgccgaca gaatttctac tttccgttcc atagttgaga cttgcgaata
    18361 tgcacgtttt gccccaatgg cagagcattc gcaaatgact gatatctacg agaagacatt
    18421 ttcgctaatt gaggatctgg aagatactct aaaacgtaag taaatgtgac attaaaaact
    18481 ttcaggaaat gaaacgcatt ctatacttag cattaatgat agttcctgtt agcctgtttg
    18541 gaaaggtaac aatgcaaaat cccgattcac ttttcgcatt ggctaataaa tactacactg
    18601 aggggaaata ttccgaagct gttacccatt atgaagctat tgtggcaaac ggaaaggtga
    18661 gcgctgagct ttacttcaac cttgggaatg cctatttcaa gctaaacgat atagcacacg
    18721 ccattctcaa tttcgagagg gcttacctgc taaaccctgg cgatgacgat atctctttta
    18781 acctcgaact agcccgtgcc tacactactg acaagattga ggctatccct gattttttcg
    18841 tggttaagtg ggtaaagtcc atttccaacc tatttagctc taacacttgg gcttttcttg
    18901 ctatttcatc atttataatt gcactgattt tatttatgat ttttcagttc tctggtaaat
    18961 atttaataaa gaaatacgct tttatcattt catggttttc tgcttttatt ttcattgttt
    19021 cacttgcttt tagtgtttcg caaaaaaata gaattgtaaa ttccaaccac gctattgtaa
    19081 cttcctccgc cgtttcggta aaaagttctc cttcggagag ttcaaatgac cttttcattc
    19141 tccacgcagg aacaaaggtc gagaccttac gcaatgttgg cgagtggtgc gagattcgaa
    19201 ttgccgatgg caataaggga tggttaccca aatcaacatt tgaaagaata taattctggc
    19261 aataggtaag gaaaaggtgg atgcctgttt taattattag cgcgctcttt ggggtgctct
    19321 gaaaaaagtt ttgcaaaacc ttaaaattta ttatcaacct tattataagg gttgatggtt
    19381 ctaaaatcgc ttggaatttt acatttcttt tcctttttcg gttttcatta aaacattttg
    19441 tcccattttg tgtttacctt ttacaactaa aaagtaaaca tcatgaaaat tttaggtatg
    19501 ataattctgt tggctacaac acttagcacc acaaatgcac aaaatgtaag tttccatagt
    19561 tttgtggtaa ccgatatcga tggcaacgag tttcctctct caaagctgaa gggaaaaaag
    19621 gtaatggttg ttaatactgc atcaaaatgt gggtttacac cacagtacga ggatttacaa
    19681 gcgctttatg ataagtacaa ggataaaaac tttgttatta ttggatttcc ggccaataat
    19741 tttatggggc aggaacctgg aaccaatcag gaaattaagg agttttgcac tactcgtttt
    19801 agtgtatcct tccctatgat ggcaaaaata tcggttaagg ggaatgacac ccatcccctg
    19861 taccagtggc ttacccaaaa aagtatgaat ggtgtaatgg atagcgaggt taaatggaat
    19921 ttccagaagt acctgataga cgaaaatgga aaacttgtcg atatgatacc accaaaggaa
    19981 aaaccaaaca gcgataaggt gattaaatgg attgaaggga attaagtaaa aaggcctcac
    20041 ttacgaggcc tttttctttt gtctgtcctt ctctcgttta taatttttat tggcaagctc
    20101 catagtggtt aggtacccta aatgtacaac cttttcaaaa acttcagggt taagggtttc
    20161 tggcttatca gtagcttgat gcagatactt gtatccacca ctggtgttga agtaaagcgt
    20221 aggaatacct gcttcgtaaa aaggttgggc atcggcacca ccgccaccaa atgtttttgg
    20281 gcttagtagt ttggtgtttc ttttatcaat ctttttggct tttttccaaa gcttcggata
    20341 gctcaacctg ccaccaatgg caatgctatc gccttgccca atacagtcaa agttgagcat
    20401 ggctaccacc ttatcggcat taatgggcat atttgcaaca aaatatttcg atccttttaa
    20461 gccggattct tctgacgaaa aggccacaaa aacaactgaa cggaggggct tcgtagttga
    20521 tttgtttatt gcttcggcaa ttgcaattag cccggcaacg cccgatgcat tatcatttgc
    20581 cccaggaaag taaagcttat cgccctggta gccaacatgg tcaaggtgcg ctcccaggat
    20641 aatatattca tgcttaagct tagggtcaga gccttcaata tagcctacaa cattaaaagt
    20701 cttttttgct tcctggtaaa cggctcgtac gttgatatag atcgatttat cggtttcaac
    20761 ggagtggggg gctttgtcgc gttttatcat ttcatagaaa tcggcgggtt tgtagggtaa
    20821 cttgctaaaa aggctatcgg taaacctact gccggcatgt atcattggga aatcagctag
    20881 atgtggctta gggccacagg ctatgctccc atataccatg gtgtttggtg tcatgtgggg
    20941 ttcaccgata aagattatgg ctttagcacc taattctttt gctattctag cctttccccg
    21001 tggcgataca tcgccccaac taccggaact ggcttgccag ggtggggtcg atttgaatac
    21061 tataacaatt ttattgcgga catcaatact tcggtaatcg ttgtattcat cattttttat
    21121 accaaatccg gcaaaaacag tttcacctcg tacttcccct gaaccggtaa atcctctaca
    21181 tacaaaatcc tctcctaaat acatgggttg tgtagggcga ttgttctgat ctaatagaaa
    21241 tacccttgca tccagtatgt agttaacttc ttcatcaaac tcctgcaaca tggtgttccc
    21301 caacaatgga tttaaaccgg catttttaaa ccttgtagca acgtaacgtg aggcctgctc
    21361 atattctacc gaaccaggca gccgcccttt aaatcgctct gacgataaga atttaacggt
    21421 gttcatcaaa tccttgctgt cgataggact ttgccgaatt tttgtttggc ttatagccaa
    21481 atcatgggtt actagtattc ctaccagaaa aacaagatat ttccacatcg cattcatggt
    21541 tttagagcct aaatttagaa attttatggt aatattttat ttgcgtaaat ttgcagagca
    21601 atagtaatat tttcaaatgg ctcccgactt tcttcaatcc gatttatatc tgtatggtgt
    21661 tttaccattg ctcatctttt ttgctcgaat atgcgatgta accataggga cattgcgtat
    21721 tatttacgtt tcaaagggtc aacgccgcat tgctcccatt ttaggatttt ttgaggtgtt
    21781 tatctggatt gttgccataa gccaaatcat gaagaacctc aacaattttg cctgttactt
    21841 tggctatgcc gctggttttg ctacaggcaa ctatattggc atgctggtgg aggagcgatt
    21901 ggcttttgga aaggtattgg tcagggtgtt ttcaaccgat aatggagccg atcttgttca
    21961 gatcctcaat gccagcggat ttggtgcaac atcctttcag gctcagggcg cacagggtga
    22021 ggtaagtatt atttactccg ttattaaccg gaagaatctc cctgaggttc agagtcttct
    22081 tttaaacttt aacccaaacc ttttctatgt ggtagaggat ataagaaagg taaataaggg
    22141 aatttttcag gcaacagctc agccaaaaat atccattttt gagcgttggc gcaagggaaa
    22201 gtagattacc ttaccctatg aggcatccct tccggtgttt caattgaaat ttctaccatt
    22261 cctgccactc taccgttgct gtaccaagga gtttggtgta ttagtttttt aactccattc
    22321 ttttcaatgg tatatgtgtt tgattttcct gtggccataa gctctttgat tattcttact
    22381 gaattgtcgg aatggcagtc aaaaagattt tttccaataa gttcctttcc tccccagcgt
    22441 tcaaaaacct tttgcgattt ttcgttcatg taaagaatag ttcctgctgt atcgcaaatt
    22501 gttatggcaa agtctacacc atttggccag cttaataaat ctgtattcat tgaagtgctt
    22561 tttttacaag gcaaaggtaa tcttaaaccg agattagtca atagtaacac aataaaacat
    22621 ttaaacagct tgtttctact ttacttatag gggtaatccc gacttatcaa aacctttcat
    22681 tccgagatct cggaatgatt agattttgtt agtagattcc tcaattgaaa attaaattgt
    22741 gaatattctc taaaaatgtt tacttatccc catttttttt caatccattt taatttttta
    22801 tttcatggta cttaggtttt gctgttcact tctcactttt catttttcac atttcgcttt
    22861 ttactattcc tctaacttcc tctaacttcc tctaacttcc tctaacttcc tctaactcca
    22921 tcatattctg tctattccga ctcattcccc taaaattagc ctggttgaat ttgtttattc
    22981 atggtacttg ggttaaattc taatggtgat tgagtttaat gatagaaaca aacgagcggc
    23041 tcaaattttt tactatcttg catggagtaa aaaaaatgat agttgaaaaa ggtaaattct
    23101 ttacgtacac ccatttacag ggattcagga ttctggctta aggacatccc aactatgaaa
    23161 aaggcgtttg ccgatgaggt aaaccaccct gctgacccag acctatatat ctactcccgc
    23221 taccgtaatc ccaccgttgt tcaaactgaa caggaacttg ccaaaatcga aggttctgct
    23281 tgggctctcc tagctcaatc aggattagca gcaattgata ttgccctttc catttttcag
    23341 gtaaatgctc aaccggtgaa gtggctgttc ttctctgaaa tttacggggg tactaatacc
    23401 tttatcgata aggttttagt tgcgcgtcgc ggattcaata ttgttaggtt tacaccaacc
    23461 aacgattcat attcgctcag ccagtttgaa aggttgatgg aagagcaaaa gcctgatgtg
    23521 gtgttccttg aagctatttc aaaccccatg ctcattgttg ccgatgtgcc tgaaatagtt
    23581 aagatttcaa agcgatttgg ggcaaaggtt attgtcgata acacatttgc aacaccatac
    23641 atcctaaaac cgttagattt tggtgccgat ttggttatcc atagtgcaac caaatacctg
    23701 gcagggcacg gcaacctctc agccggagtg gtttgtggta acgatatcga tttgcttaaa
    23761 caagcaattg agtaccgcaa gctggttggc catatgattt cccctgacga tgcctatcgg
    23821 cttagcgatt acctgaaatc gtttcacttg cgtatttcac agcatttttc gaatgcacag
    23881 cgtatcgcag agtttctttc aaagcaccct gcggtggaaa aagttctcta tccctcaatg
    23941 ccctatcatc caacaagcaa cattgctagc aaaatatttg gcaatgggta tggtggaatt
    24001 gttacctttg acattaaagg caattccttt acccataaac aagagaattg caacactttt
    24061 atccagttgg tttcgcacaa tattcatctc attccaaccc ttggagatgc tgacacaata
    24121 ctgatgcccg tggaaccagt gtggggcgac aagtatcctt tccctggaat ggttaggttg
    24181 tcaattggca ttgaactccc cgagagctta atcaatgtgt taaaaaatgc actggacaaa
    24241 ataagttgtt aacatgctaa cgattgcaaa aagttaaaat attttaatgt tatattgata
    24301 acaagtattt tgcttatgat tattttattg attattaggt caatgcgcta taaaaacatt
    24361 gatttgattg ctaattttta ggtaaaatca taacaattgt catatttttg cctataacct
    24421 aaacattatt tttgccgtaa caggttgcag taatttgaac caacataatc accgaaaacc
    24481 ctaaaaacac ataatacagg aggaataagc tatgcagaat aagcttcaag agttaaccga
    24541 gaagatatat cgcgaaggtc tggaaaaggg acaggctgaa gctaatcagc ttgtgaccaa
    24601 ggctaaggat gaggcagcac gtatagtggc caatgcaaaa gctgaagccg aaagaattat
    24661 agccgctgcc cgcaaggaag ccgacgatta ccgcaaaaat atcgataccg aggttgctct
    24721 ttccgccaag caggttatca gcggggtcaa acagcaaata gtttccttaa ttgaaaccaa
    24781 actttacgaa gccccactgg ttgacgccct aaaggatgcc gattttgtga aaaaggtaat
    24841 tgaaactgct attaaccgtt gggaacctaa ctcagctgag agcgttagcc tgcgtgtact
    24901 tgttcccgaa gaaatggcta aaaccattac cgaccatttc accaccaaaa cccatgcatt
    24961 gctcaacaaa gaggtcgaaa ttcaggttga tagggctata aagtatggtt tccgcattgg
    25021 tccaaaggat ggtagctacg tggttagctt taccgaaacc gattttgaga acctctttaa
    25081 ggacttcatg cgtccaaaaa tagttgcaat gctttttgga ggtaagtaga atgatgcgaa
    25141 actactacta cctggtggct ggcctcccgg aactgattat ggatcaggac cgaaaggact
    25201 tcagcgtaaa cgctttgaaa caggaagtta aggaacaggt ttcctcttcc gattaccgcc
    25261 tggtagagtt cctgcacttg ccctacgata acgacaactt tctcaataag ctgctcaacc
    25321 gtggacttga gttcaacgac ttgggctcct acgagaaatt tgtttttgat gagctcgatg
    25381 agaatattaa tcagctccca caatacatgc aggattttta ctaccgcttt acaggcaaac
    25441 agcgctctgc cgatgatgcg gttgaccagg atgagcagcc aaaggatatt tttgaggttg
    25501 aaaaacttcc tgagattagg ttcctggaat ctttttacga ccttgccctt agccaaaaca
    25561 acgattttat aaggtactgg ttcagcttca ctcgcgattt taacaatgtg cttaccgcgc
    25621 taaactgtcg caggcttggg gtcgatcctg ccacccaact ggttggtaat aactggctta
    25681 ctgaggttct ggttaaaagc caggccgctg attttggcct aaagcgcgaa gtcgactata
    25741 tcgataggct gatccaggct accgatatct ctgatgtttt ggagcgtgaa cgtaagcttg
    25801 acctaattaa gtgggacatg tctgttgagt ttactacttg ggactacttt aatatcaact
    25861 ttatacttgg tttctttgtt cgtgccggta ttgtccatcg ttggctaaag ctcgatgcca
    25921 agaccggtga ggaacttttc aggaaacttt tcgatgatct taagtcatcc tacaatctct
    25981 ccgagaaatt ttaaagcgat tattaattaa caattaacat aatgaaaact caaggaaaag
    26041 taaccggaat aatttcgaac cttgtggttg ttgaagtcga tggcccggtt gcccagaacg
    26101 agatttgtta tatcaatctc gctggcacaa agcttatggc cgaggttatc aaggttattg
    26161 ggaaactggc ttacgtgcag gtgttcgaga gcactcgcgg ccttagagta ggaactcctg
    26221 ttgagttcca ggggcacatg ctcgaagtta ctcttggccc tggcctcctc tccaaaaact
    26281 acgatgggct tcagcacgac ttagataaaa tgaagggcat tttccttcaa cgaggcgatt
    26341 atactgacgc cctcgatttt gactcggaat gggattttac tccacttgtt gagcccggtg
    26401 ctcaggtaag cgccgccgat tggcttggcg aagtccccga aaactgggtt aaacacaaga
    26461 ttatggtgcc tttcaccttt accgacaagt acaccgtaaa atcaattgtt cctgctggta
    26521 agtataaagt atccgattcc attgcagtcc ttgttgattc taaaggtgaa gagcataagg
    26581 tgaccatgta ccaaaaatgg cctgttaaaa aagctatcac agcttacaag gaaaaacccc
    26641 gtccattcaa aatacttgaa acgggagtta gggttattga tacccttaac cctattgccg
    26701 agggcggtac tggctttatt ccaggtccat ttggctgcgg aaaaactgtg ctacagcacg
    26761 ccttggctaa acaggctaat gccgatatga ttattatggc agcctgcggt gagcgtgcca
    26821 atgaggtggt tgaaattttt actgagtttc ctcacctcga tgacccacgt acaggtcgta
    26881 agctaatgga acgtactacc ataatttgta atacatctaa catgccggta gctgcccgtg
    26941 aggcatcggt ttacaccgca atgactattg ctgagtacta ccgctccatg ggcttaaagg
    27001 ttctgctttt ggccgactct acatcccgct gggctcaggc tctgcgtgaa atgagtaacc
    27061 gtcttgagga acttcctggt cccgatgctt tcccaatgga ccttccagct gtaattgcca
    27121 acttctacgc ccgtgctggt ttcgtttacc tcaataatgg cagcactggt tcagttactt
    27181 tcatcggtac cgtttcgccc gctggtggta accttaagga gcctgttacc gaatcaacca
    27241 aaaaggctgc ccgctgtttc tatgcgctct cgcagcaacg cgccgatagt aaacgttatc
    27301 cagccatcga cccaatggat agttactcta agtaccttga gtaccctgaa attcaggaat
    27361 tcctgaataa gaatgtcaat gaaacctggg ttgatatggt aatgaagaca aagaatatca
    27421 tacagcgagg tagggaagcc tttgaacaaa tcaacatact tggtgacgat ggcgttcctc
    27481 tcgactatca cgatcgttac tggaaggccg agatcattga ctttgttgta ttgcaacagg
    27541 atgctttcga taaaattgat tgtaatacgc cattgaagcg ccagcgatat atgcttgaaa
    27601 aggttatgca gatatgtgat actaacttcc atttcgatgg ttttgagcaa tgctcatcgt
    27661 attacaaacg cattatcaac gcgcttaagc aaatgaacta ctctgagttc cagtccgaag
    27721 agttctataa ctacgagaag gaacttgaaa caattcttaa cgaaagaagg gtacagtaat
    27781 ggaaactaaa gcatttcaga gaatatacac ccagctaacg ggtatcacaa aggcaaccgt
    27841 atcgcttcgg gcccaaggtg tggctaacga ggaattagct atggttcacg gtcggttagc
    27901 ccaggttgta aaaattatgg gtgatatggt taccctacag gtgtttagcg gcactgaggg
    27961 tatccctacc aatgccgagg ttgttttctt tggcgaaccc ccaaaactta aagtaggcga
    28021 cgatttggca gggcgctttt ttgacgccta tggccgtccc atgtttggcg attcggagat
    28081 cgagggtgaa ccccgtgaga ttggtggccc ttcggttaac ccccttcgcc gtaaacaacc
    28141 ctcggagctc attgctaccg gtattgcagg tatcgaccta aacaatactc ttgttactgg
    28201 tcaaaagata cctttctttg ctgaccccga ccagcctttt aaccaggtta tggctatggt
    28261 agcactccgg gctcaggccg ataaaatcat tcttggcggc atgggactct caaacgacga
    28321 cttccttttc ttcaagaatg tgttcgaaaa tgctggtgcg ctcgaccgta tcattagctt
    28381 tgttaatact actgagcagc cccctgttga acgcttgctt gtgcccgaca tggcattaac
    28441 cgcagccgag tactttgcgg ttgagaaaaa ggaaaaggtg cttgttcttc taaccgatat
    28501 gaccctctac gccgatgcgt tgagtattgt atcgaaccgt atggatcaaa tcccttcaaa
    28561 ggactccatg ccaggatcgc tttattccga cctagcaaag atatacgaga aagccgttca
    28621 attccccgat ggtggttcaa tcaccattat tgcagtaact acactatcgg gtggtgatat
    28681 tacacatgct atccccgata acactggtta cattaccgag ggacagctct tcctccgtac
    28741 cgatagcgat acaggtaagg ttatcgttga cccgttccga agcctctcgc gtttgaaaca
    28801 gcttgtaatt ggtaagaaaa ctcgcgagga tcatcctcag gttatgaacg ctgcagttcg
    28861 tctgtatgcc gatgctgcaa atgcaaaaac caagctggaa aacggcttcg accttactga
    28921 gtacgatgag cgtgccctta agtttgccca cgactattcc gaaaagttgc tggcaattga
    28981 tgttaacgta gaggttgata ccatgctcaa tactgcatgg cagctctttg ctaagtattt
    29041 ctctaaaacc gaagtggcca ttaagaacga gctggttgag aaatactggc ctgcaaacta
    29101 ataatgtgct aaaaacatgg caattaagtt tcagttcaat aaaacatcgc ttaacgagct
    29161 caataagcag cttaaaatcc gccaaagagc tttgcctacc ctaaagaata aggagtcggc
    29221 tttgcgtatg gaggttaagc gcgctaagaa taggatgcag gaactcgagg aacatctggc
    29281 taaacgcatc aaggattatg agtatatggt tagcctttgg ggagagtttg agcgtaacct
    29341 gattcagatt gacgatgtta ggcttggaac tatcaaaatt gccggggtaa aaatacctgt
    29401 tctacaagag gtaattttta gtgttaaaga gtatagcgtt tttgcgcgcc cctactggta
    29461 caccgatgga gtaaaaattc taaaggagct agccgaaatt ggtattgaac gcgatttcta
    29521 ccacgaaaaa atgcggctcc ttgaccatgc gcgtaagaaa actacacaaa aggtaaacct
    29581 ttacgaaaag gttcaaattc ccggctatga gaacgccatt cggaagatta agcgattcct
    29641 cgaagatgag gaaaaccttt cgaagtcggc tatgaagata gtgaagaaac gatacgaaaa
    29701 cgtggaggtt gagtcatgat aacaccaatg tataaataca acttcctggt ttatcaccgc
    29761 gatttcgacg atttcctgct taaacttcag gatttgggcg tggttgatat ttccatggaa
    29821 aaccgaaacg ttgacgagga tgaaaaggag attctggccc gaatcaatag gatagctact
    29881 gctatcaagt ttcttgaagg ccgaaaagcc cctgacgaaa aaatcacact cagcctttca
    29941 gccgatgaaa ttgtttcgcg tgttgagttg cttcaacgtg aacgggagca atttgaaaat
    30001 gctattaaaa aagcccaaaa ggaattggca gaggttgaac cgtggggtaa ttttgacttg
    30061 aaattgctta acagccttgc cgaaaaaggt atttcaattc gatttttcat tgcacccgag
    30121 aaagttttta atgaggactg ggtaaatgag tatcctattg aaatagttaa ccacaccaat
    30181 ggactatact actttgtaat ggttaattac acaggcgagg ccatttcgcc cgatgttcag
    30241 gaggttcgaa cacctcagtt ctcagttaag caaaaagatg aagaaataca tcgctaccat
    30301 aaacgtatcg atgaaattaa caccgaacta gattttctgg ctctttacat cgatttgctt
    30361 aaaaaagagc agattgaact taccaatcag ctcgatttta aaatggttaa gtcaagcgca
    30421 acggccgaag caggaaatac cataatggta cttgagggat gggtgcctga ggatttgaat
    30481 gaaaaggtaa aagaagttct tgatgccgaa agtattatct acatttcgtc taaggccgat
    30541 aagaacgata acgcgcctat tctacttcga aacagtaaat tcgcaaagct gtttgagccc
    30601 attagtaaac tttttgcctt cccaacctat gcagaattgg acttaacccc attctttgca
    30661 ccattcttca tgctcttctt tgggttctgc tttggcgatg cgggttacgg tttaatcttt
    30721 gtcttaggtg ccacaatcta taagtttaag gcaaaaccag aacttaaacc tcttctttct
    30781 ttaattcaga ttcttggaat atctactgtg ttgtttggag cactaaccgg tactgtgttt
    30841 ggcgctagcc tggttgagtt agatattcct gctttggaga gctttaaatc ctacttcttt
    30901 gaccaggata agatgtttaa gttatccctt atacttgggc ttgtgcagat tgtttttggc
    30961 atgtgcgtta aagccgctaa cctttgggta cagttttcat ggaagcatgc catttctact
    31021 attagttggg ttgtattaat cctcagcatg gctgttttct accttttagg ccaaaacccc
    31081 gaaagttcat tgaaactttt cagcccaatc catatggtta ttgttggtgt tgcgggcgtt
    31141 gggataatgt tttttaactc acccggtaaa aatcccctat tcaactttgg attaggcctt
    31201 tgggatactt acaacatggc tacaggcctt ttaggcgata ccttgtcata cattcgtctc
    31261 tttgcccttg gtctgagtag cggtatccta ggtagtgtgt ttaatagcct tgcctttggt
    31321 atggcccccg atactccggt gctcaagcat attgttatta ttctgatatt gcttattggc
    31381 cattccatta atattttcat gtctgcatta ggttcacttg tacatcctat gcgtttgaca
    31441 tttgttgagt tttacaagaa tgctggcttt acaggcggtg gtagagggta caatcctttc
    31501 cgtaagaata cggctgaagc ccaataaatt tgaaatcgat ttataattta aataacctaa
    31561 taaattttaa actatggaac caatcatttt tgcttactta ggaattggga taatgattat
    31621 cctttctggt gttggaagtg cttatggtgt taccattgct ggtaacgcat cggttggcgc
    31681 tttgaaaaag gaacctggtg catttggtaa cttccttgtt cttagcgcgc ttgcaggaac
    31741 acagggtctt tatggcttta ccggttactt tatgctcaag aaattcctta tccctgaaat
    31801 cacatgggct caagctgctg caatttttgg tgcaggtttg gccatgggat ttgtagggct
    31861 tttctcagca attcgccagg gacaggtttg tgctaacggt attactgcca ttggtaatgg
    31921 gcacaaggtg ttcgtaaata cccttatcct tgctgtattc ccagaacttt atgcaattat
    31981 ctctttggct gctgtatttc taatcagcac ctcattgtag ttgaaattct tcacttatct
    32041 aataggcggc ttccttttgg gtggccgctt tttactttgg gtaaaaaggg atttactttc
    32101 gtttttatac caaaagaaat agctttgctg tttgcgtttt tcttcaacgc ttcgggctac
    32161 gtaaaccttt tcgccagtat agggatttat acctgtatag taaattgttg atgctagggt
    32221 catgggagtg ggcgtgaagt cctgaacctg ttcaagcttg aagtttagct tacgggtttc
    32281 ttcggctaac tgcttcatgt cttgttctgt tgaccctgga tggctcgata taaagtatgg
    32341 aattaattgc tgtttttggc caaatttttc gttcaaagca tcaaaccact gcttaagctg
    32401 tttaaacagt tcaaacgatg gttttcgcat tatttttagt actttttccg atgtatgttc
    32461 gggtgccacc tttaaccgcc ccgatacatg atgtagaaat aggttttcaa aatattcccg
    32521 ctcggcttta ctatccttcc gagttataaa taaatcgtag cgtaccccgc tgcccacaaa
    32581 cgcttttttt attccttcta ctttacggac ttgattgtat aggttgttta taggctcatg
    32641 gctcgaattt agatttttac agatattcgg gtagatacac gaaggccgtt tgcatttggc
    32701 acaaatgctt aaatcctttg gacccatccc atacatatta gccgatggcc caccaaggtc
    32761 actgaggtag cctgcaaaac cagggatttt ttttatttcc tcaatctctt tcaggataga
    32821 ttgctctgaa cgcgacgaaa taaaacgtcc ctgatgggcc gatattgtgc agaaactaca
    32881 ccctccaaaa cacccccgat gtatgtttac tgagaattta atcatctccc acgcaggaat
    32941 ggttttccct ttgtatcgcg gatgtggtaa tcgggtataa ggtaaatcat accatctatc
    33001 aatttcatgg gttgtggcag gattaaaggg cggatttaca actacacacc tatcctctat
    33061 gggttctata agaattttac tgtttatcct gttcgattcc tgctcaatta tcttaaagtt
    33121 attggcaaat ttgagtttgt cggctaaaca ctcctcgtat gaacttagta ttagaatatc
    33181 ctccccacgt ttatacttat caatattgtg gtcaatgtaa gcaacttggg gtatcccgcg
    33241 tagcgattca attgccatac cctggtcaag ctttttagca atttcaataa tggctttttc
    33301 acccattcca taaaccagta tatcggcttg gctatcaact aaaatacttg gtttgagggt
    33361 atctgaccag taatcataat gagttaatct acgcagcgaa gcctctacac caccaataat
    33421 caaaggggtg cttgcatata gttttttgat tattcgggag tatacaactg tggcataatc
    33481 gggtctaaaa cctgctttac catctggggt ataggcatca tcgctcctta atcttttagc
    33541 ggccgtatag tggtttacca tggagtccat attaccggca gtaacggcaa agaaaagcct
    33601 tggctctcct aattttttaa agtcacgcag gtcgtcgcgc cagttgggtt ggggtacaat
    33661 agcaacctta aatccctcgc tttccagtac tcgtccaata acagcagcgc caaatgcagg
    33721 atgatcaaca taggcatcac ctgtaaatat tatgatatcg ggctgttccc agcccaatgc
    33781 ttttatttct tttaccgatg tgggcaaaaa tcccatttta tgttgatttg tttatatgaa
    33841 gtaaactact atcgccatag cttaagaacc taaaattgtt gtctaaggca aattggtata
    33901 tttttttcca atcgttgcca actgcagcag caacaagtaa tagaagggta gagcgtggtt
    33961 ggtgaaaatt ggttattagg gcatcgcagg tttttatttc ataaccggga gcaataataa
    34021 tttgtgttga agccgataaa gtctcaatgc cattgttttt catgtgttcg taaagagcct
    34081 tataggcttc tccaacgtct atttgaaaat caaactcata tggttcccat tgcgaaatgt
    34141 taaagttccc atttaacttg ccaagtttta atttaacccc aagccaatac aacgattcca
    34201 aggttcttaa ggtagttgtt cctacgcata caaccggctc gctattttga tatagtcttt
    34261 ttactagttc tagactaaca aaaaagtgtt cggtatgcat ctcgtgaccc tcaatggtat
    34321 cggatgttac aggtttaaaa gtgcctgcac ctacgtgtag ggttatgtag tggggtttaa
    34381 catgaatttt acttaaactt tcaaaaacct ttggtgtaaa atgaagaccg gcagttggcg
    34441 ctgctaccga tccttcgggc tctgcatata tggtctgata acggtagccg tcaatatcct
    34501 cgctttcacg gtttaggtaa gggggaatgg gcatagtacc gcatttttcc acaatatcgg
    34561 caaatgacat agaactgttg ttccacaaga actcaacttc tacgccttcg ctaagccttt
    34621 ttagctgttt tgccacaagc cttcctccag taccttctat ttccatttga agttcgcctt
    34681 gcttccaacg cttaaggttg cccacaatgc atttccatac cacctttttc ttgctcgaga
    34741 acgaaagagc gtagtcactt ggctcaatgg gttcaattag aaatacttca attgttgccc
    34801 cactttcctt tttaaacctt aaccttgctc taaccacctt agtattgttc agtataagta
    34861 agctttttgg ggtaatatat tggttaatat tacggaaggt atcatttgtt atttcaccgt
    34921 tctggtaaat aagaaattta gagcagtccc tctcagcaag aggatgtttg gctatccttg
    34981 aatcgggcaa ctcataatca tagtctgtaa tacttatttt cttaactttc atatcccttt
    35041 tttgactcta aggccacaaa attagttatt tttgtgggtt aaaaagattt gtgagtaatt
    35101 ctgaggagtt ttattatgaa tgtagctgta ggatgtaagg ttcgtttttt aaacgatgtt
    35161 ggagggggtg aggttattcg gatcatcgat aagcgaacgg ctttggtcaa aactttcgat
    35221 ggttttgaga tgccagttct tttatctgag ctggtagtgg ttgaaccggc tgccgatgaa
    35281 ctcctgcaga aaaaaacagt tcaggagaag gaaacggctg atgcaaatcg caacaaaaag
    35341 ccaaagtctg aagttatcaa tctaaccgat gacacaccag aggttgctga tagttcaggt
    35401 gatgaactct cgttggatat agcttttgtt cctagtaata tatccaacct acagcaatcc
    35461 actttcgata tgtatttggt taacgatagc acctataggg tattctatgt actttcaaat
    35521 gtgattgacg ataatcatac cccaattagc tcaggcatac ttcagcccga tacaaaggta
    35581 ttggttaaaa ggctaacccc cgaagatgta agtaaactga ttttaatgaa cctacaagca
    35641 atatactata agaacatccc atataagcca tttcagccag aattctacga tattgagctt
    35701 gaccctataa aactatacaa aaggggcaat tttaaacgca atgatttttt tgatatcgac
    35761 gcatacataa tttccattgc aagcactaag cgcgaggctt ttattcgtca cttaaccgat
    35821 aaggcaatag tagaggcaat aaagcaaaag gaaaaaccgg ttcaagtaaa agaggttaag
    35881 aaaccaaatc ctgatactga agaggtagat cttcacattc atgagcttat agacaattgg
    35941 aaggatttaa caccaggtga aatcttgaaa attcagcttg ctcgtttcga aacggctctt
    36001 gagggaggca ttaaaagcac aactactaaa cgtatggttt ttattcatgg tgtaggcaat
    36061 gggaagttaa agtacgaaat aaccaagctg ttaagcagca aatacccaaa gttaaggtat
    36121 caggatgcct cgtttaaaga atacggcttt ggtgcaacca tggtattcct taagtagtat
    36181 aaagggaaaa gagttttttg aaaccataaa ataggcaagc agatagccgt cctatcgcgc
    36241 cgtgcaatat cggtgaggaa agtccgggca gcgcagggca ccatacttcc taaccggaag
    36301 atgtccgtga gggcatagca gtgcaacaga aaataaccgc catgcctatg gcatggtaag
    36361 ggtgaaaagg tgaggtaaga gctcaccgga ttccatagcg atatggaatg ccgtgcacct
    36421 tatgggctgc aagaccatgt atactccgac ttcttacgaa acagggctgc ccgttctgtt
    36481 cccgcttgtc gggtgtcgga gggggtaggt cgcttgaggt tgtaggtgac tacaatccca
    36541 gataaatgat aggagcgttt ttaacgcaca gaacccggct tacaggttat ctgcttgcct
    36601 ttattatttt aatttgctat taattatgtt aagttttagc aagttctttt tagataagta
    36661 aaatgaagaa gggaatatct tgtatatact ttttactatc atcttaaaat cccccaaaaa
    36721 aaaggataaa tactaaaaat taacagcccc cacacctaga tttacaaatg taaacatggg
    36781 tgctttcaga aaaagatcat ttttaaaaac aatagaaatt aatggttgat acatcaaata
    36841 tatattaata tattatttat ataactgtat atcaataatt aatatgtttt atattattta
    36901 attatttaaa atatattggt ctatacacat attatagaat aaatattagt taaaatattg
    36961 aaatatactg ttcaatatca attatataca atatttattt caagtaaata tttttaacca
    37021 taatgttagc tggtttagta ccagtaattt tttcataaac gtaatagcaa gaggagtgtt
    37081 gccccatacg ttaatcagca atacctgtaa aatcaactat tactaaacaa aacaagccaa
    37141 agcattaaac cgtagtgggg tagtagttat gacttttact tatgatggtt acttaattac
    37201 gtatataaga gccttaggaa atctttttga gtttgttgat tacagcgata gtaaataatg
    37261 aagggttttt gtgccttaac atctatatga aataccgata attatatacc gttcagttct
    37321 gttggttaag gttaattatc ctgttgatag caaaatttta gttcacagga ttaatcttgt
    37381 ttcaactttt atacgaatgc ctaatggtat ggcctgtatg tcgtttaatg ctaaatcatt
    37441 tctttgttga tgtcttaaat ttggcgttta atttaaaggt atacttaacc tatgggtgaa
    37501 acacctctaa ggggattact tcattgaaaa tatagccacg ttaagaagtt gataatattc
    37561 attcacctcc ttttaaaatt aatttgtttc gcaatcggat tgctttacca aaaagcaaat
    37621 acacaacgct gcgtaacctt gggttacatg tgtgaatgtg cttattttta caatatttac
    37681 atttgtattt tgacaatact ttacgaataa ttacatttgt gaataattca agtggtacaa
    37741 tcaaaaacat ttcaattatg gttaacgaaa gattgcttaa gcttatggag caggaaaagg
    37801 taactgctgc caagctagcc gatatattag aggttcaacc cagcgctatt tcccatatac
    37861 tttcagggcg aaataagcca agttttgact ttattcaaaa gctagccaca aatttcccaa
    37921 agttgaatat tgattggctt ataaccggca agggtaatat gtacaaggtt gcagttcaac
    37981 agtcactttt cgatccgcca tcacctttaa ttcctaaaac cgataatccc agctctaaaa
    38041 ccaacgataa aagtgaagat ttgcaaaaat catcgaaagt tacagatgta aatatttcgg
    38101 tttcacacaa agctgtaaag caaattgttt ttgtttattc agataatact tttgaaatat
    38161 attccccctc aaagtccgat taactatttt tgttagtttt gcaaaaaaaa tgctttacaa
    38221 gaagttaatc agaccaatat ttttcattct aaatcctgaa ttagctcata agttagtggt
    38281 cgcaacgctt aaagttgcct ttaccattcc cggggtgcgt tttctttgca taaaaatgtt
    38341 cacggtatct cacccttcgc ttaagcgcga cgtctttggc tatcattttt ccaatcccgt
    38401 agggcttgcc gctggattcg ataagaatgc tgattacttt cccgagttag cctgttttgg
    38461 cttttctttt atcgaggttg gtactgttac accaaaacct caacctggaa atcccaaacc
    38521 acgtttattc cgtttaattc ccgacaatgc tttggtaaat cgaatgggat ttaataataa
    38581 aggtgttgat tatgttaagt tgaacctgtc aagaaagcgt cgtccgaata atttaataat
    38641 aggtggtaac attggaaaga atacgttaac gccaaaccaa aatgcaatcg atgactatct
    38701 tactgttttc catggtttat acccccatgt ggactacatt gtggtaaatg ttagttgtcc
    38761 caacgtagca aatctgcgcg atttgcagga cgatgatagt ctgaaaagct tacttaaggc
    38821 aattaatgcc gaacgaatga actacgattt ctataaacca atacttctta aggtttcacc
    38881 ggatctcacc aatgaacatc tgactgccac aattaaaatt gctgaagaag aaggaattga
    38941 tgggtatatt gcaaccaata ctacaacatc acgcgataac cttcaaaccc ctgaagcaaa
    39001 agttaaaacc attgggaatg gaggtttaag cggtaagcca ttggctaaac gttctacaga
    39061 agtaattaag cttattcata cccttaccca gggggcaaaa cccattattg gggttggtgg
    39121 cattcattct cctgacgatg caatagagaa actgaaagcc ggtgctacac tcattcagat
    39181 ttacacaggg ttcatctacg agggaccggc tattgttaag cgcattaata aagcattgat
    39241 aaatagcgat atagtttaaa ctaaagaaac caatatgaca aaaattattg attgtcgtgg
    39301 acagctatgc cctcagccac ttatcaatac taaaaaggct ctgaaagaat cggttaagga
    39361 tgaaacgatt caggttttaa tagacaacgc aacatcgtgc cagaatgttt cccgtttttt
    39421 agccgataac gcaatcccat ttaccgttga tgaacagaat ggacttttta ccctaacaat
    39481 caatccaact ggttcggaat ttaatcccaa gaaagaggca gaggaatact gcgagatcag
    39541 ttttccaccc agaggcaaaa aatacattgt tgttcttacc agccatttaa tggggaatgg
    39601 tgacaatgat ttgggaaaaa ttctcatgaa aggtttttta aacactctac ccagtctcga
    39661 agggcttccg gaggagatca tctgttacaa cagcgcagtt acacttgctg taaagggtag
    39721 cgatactgct cagagcctag taaaactaat ggaaatgggt gttaaaatca cactttgcgg
    39781 aacatgtgtc gatttcttta gtataaagga taaaattgag gtgggaacaa tctcaaatat
    39841 gctctatatt gctgaaaggc ttagctcagg aatccatact gttaaaccgt agtaatggcg
    39901 aactattact tcgataattc ggctaccagt caccctaagc cacctgatgt tgcccaatac
    39961 attaatgagt acctgaagct tggtggcacc tatggacgta gtgcatacga tagggttatt
    40021 aatgtttctc ggatagttga acatacccga atcttactcg ccaggctatt aggcacaagc
    40081 cttaccgaac atatagtgtt tacttataac gcaacccatg cactcaatat tgccataaag
    40141 ggttttccct accggaaaaa acgagtactt atttccccct tggagcataa tgcggtaaca
    40201 cgccctgtgt ttcagctgca gaatactatt ggacttgagt tcgatatcat gccatacaac
    40261 cccgatggtt caataaatct tgaaggcctc aggcaattgg atctaaaatc atacgatctg
    40321 tgtattgtta accatgttag taatgtgaat ggggttgtac agcctattgc tgagctaaaa
    40381 gctctactgg gtgaaattcc attgcttgtc gatgcaagtc agtcggcagg gcatattcct
    40441 attgaggtgg atacctggaa catcgatatg ctggcattaa ctgggcataa ggggttgatg
    40501 ggaccaaccg gaacaggttg cctgtttgtt cgcacccccg aaataatcaa cccgttaata
    40561 catggaggta ccggttcccg ttccgatagc tttctgatgc ccgacttttt acccgatagg
    40621 tttgaggccg gaaccccaaa tgttttgggt attttcggtt tatatggagc cctatcggca
    40681 aatgtaacca atagctacga gcgaaaacat tttatggacc tactcgaaag ggttgagcat
    40741 ttaagatcat ttaaagtgtt ccgatccctt gatgttaagc atcaggcatc gttattctca
    40801 ataacttcca ataaatacag cccttcagaa ctgtcggatc agcttttcaa gcgctactcc
    40861 attgaaaccc gaagtggtct tcactgtgca ccgcttgcgc accaaaccct aaggacgttc
    40921 cctatgggta ctgtcaggtt ttcgctttcc aaataccata attcaaccga tttgaattac
    40981 ctttactctg ccctgaaaga gctcgatcat ggatgaacgg aaaggtaaat gtttactcct
    41041 ttttcagagt attcataagg taatgcaagc tgagcatatt attgtaggct taggcgtgca
    41101 gtatcaaata attccagtac ccaaggagta cagctctgaa tgtggtatgt gtattgaaat
    41161 tgattgttct gatagtaata atctcttagc tgaattacaa ctaaatagat tatcgtttaa
    41221 catagtaata gaagtagatt catgagtacc aatttcgact tgttaaccac tgtggagtac
    41281 ggtggctgtt cggctaagat tccagcttcg gtgctcgagt ccattctgct taacctgccc
    41341 aaagtgaccg atcccaatgt attggtagat attgataccc acgacgatgc tggggttttc
    41401 aagataaatg aggatttagc cctaatattc accaccgatt ttttcccacc tgtatgtagc
    41461 gatccgtatg agtatggaca aatagccgca accaattcat tgagcgatgt ttacgccatg
    41521 gggggcgaac ctttccttgc gctaaacata gccatgttcc cttctactaa aattccttta
    41581 gatgcctaca ggcaaatcct tgagggtggg gcaagtgtag ctcaaaaagc aaatacaata
    41641 attattggtg gtcacaccat cgacgactat cccccaaagt atggtttagc cgtggtgggt
    41701 agagttcatc ctgataaggt gattaccaat gccggcttac aacccggcga tgctttgatc
    41761 ctgaccaaac ctttgggcac tggtgtagcg gtagccgcta agcgcttagg aattgtgtcc
    41821 gaagcggctt acagcgaggc ccttttctgg atgaaacagc ttaacaatac tgccgcgcag
    41881 gtcatgcaaa ccgttggtgt taaaggtgct accgatatta ccgggtttgg gcttattggc
    41941 cacgtataca aaatggctaa ggcaagccgc gttggtgtta agctttacac aagcaaaata
    42001 cccctgcttc ctgaggtata tcagcttgtg gatgagggct gcattcccgg tgctgcattc
    42061 cgtaatctcg agtttgtagg cagccatctg actggtgacc caaatgttga ctacaatatt
    42121 aaaatgctta tgtgcgatgc tcaaacgtcg ggtggaatac taatgggtgt taagcctgaa
    42181 agggttaatg aggccctaag tcttttaaaa gaggctggtt taacttattc ggcaatagta
    42241 ggagaggttt ttgaaggcga taatattttg attactagtt agatattgtt gtttctggaa
    42301 ttgatatgtt aatatttttg aatcaaactt taattttatc attcttatca attattttac
    42361 gataattcat tgtaaaacaa catactgttt tttatgattt ttagggaagg ggagtggtat
    42421 aattttaaga ttactaaagt aattgatgtg cctggaggcg gcaagcaatt cgttctttcc
    42481 catgaatcgg gcaaaaaatt gctattgccg gctcaatatt atgataagta caatatagca
    42541 gaaaactcta ccattcaatg caggatagat aagataaact gcacgggtca aatttttatt
    42601 gagccagcgc atccggttta caaaaccggg ggtacatata cttttaagat taaacatatt
    42661 ggcgatactg caattgagga ggtgcagagt ataattgtat gcgatatttt tggtaatgaa
    42721 atagaagttt tagctcctaa agggaccatt gatgatagac aagaggaggt aaagcttaaa
    42781 gtgcttagtg ttaaaaaggg tgttcctatc ctttcagcaa ttgctaaatg ggacatttgc
    42841 cccgatacct taagtaatgg acaggagata ttgctttttc taagtaaggt agtagttatt
    42901 cagaacgagg agtattacct gctaaaatca aaatcgaatt gtatagcctt gctaaaggta
    42961 aagcactata aacactatgg tttcaaagaa gggattccta ttgttgccag atatagaggt
    43021 actgataaca gaggattttt gatgattgac ccagaacatc catactataa ggtgggtgga
    43081 gtatatcaat ttgagattgc agcaattgaa gaggattata gtaatgaact aggtagtagt
    43141 agggttgcgg tagtgtatga taaatttggc atgaagtgtg gcttacaaat tgagaatgcc
    43201 tcatcgttca gtgtaggaaa aagcgttagt tgcaaagtta tagggtacaa aaaaggacga
    43261 cctctactcg aaatcgtccg catgtagctt aaattaagaa aattactcgt cggctatctc
    43321 ctcagcaggc tcctcatcgc taatatcatc agggaattca tcgccctcgg ttccgatggt
    43381 atcatcggtc aagctgccga aaaattcctt ctcctcatcc tcatccttta tctttgtgtc
    43441 aatttttact ggtaccttta ccaggtaaat ggcatcctcg gtttcaaggg taacggcatg
    43501 gaaaaaatcg ccatttggct tctcaacctt gaaaacaaag tcctgatacc catgggggta
    43561 tctttccttg aatattttta aaagctcagg agacaaattg ctaagactta ctgctgctct
    43621 tttcttactc ataaatcgct taaaattttc cgcaattata gtgcaaaaaa agaaattgca
    43681 aggggagttg tgaatatttt tttcaaaaaa tattatataa cacaatatca gagtgttaaa
    43741 ataggatttt ttgcttttga atatccgatt ccaatatctt aatatctttg agtgttctaa
    43801 attaaatact atgaaacgat tgttgctact aaatctatta attttattgg gattgatggc
    43861 taacgcccag gtaaacatat ataatcccaa tgccgacgct aaggctgaaa ttgcagccgc
    43921 aattaacaaa gcaaagcagg aaaataagca tgttttcatt caaattggag gcaattggtg
    43981 tccatggtgc gttaagctcc ataattttat gaacgcagat tcaactatat ccgaaatcct
    44041 aaccaaaaac tacgttgtgg ttaaggttaa ctacagcaag gaaaataaaa atttagatgt
    44101 tcttgaaaag cttgaaaagc cacagcgttt tggttttcct gttcttgtga ttttaaattc
    44161 caatggaaac agaatacaca ctcaggattc gggtttgcta gagtccggcg atggttacga
    44221 ccgaaagaaa gttctgaatt tccttaagaa ctggactcca gaagcactta aataaaagtc
    44281 atataattaa tattatgtta agtaatatgc tttaaataaa agccccactt cggggctttt
    44341 attaatttat cagaattaca tctgttttgc cttttcgttg aattcattgt acttatccaa
    44401 catttcctgg ctctcgttac ggaaacggaa atagatattt ttcaaagctt caacagcttc
    44461 cttgctattg ggatttactt tatatgcttg ttccatgtaa ggcattgagt tcttaaattc
    44521 tgcattagcc ttttccataa gagcatcgta tttatcaagt tcacgagcag gaaccttatt
    44581 ggcttcctct atgtacttta cacctttatt gaagtaaagc gcaccaatat tgtagtaagc
    44641 atcaaagaaa ttaggatcga tctcaatagc ttttttgtag gcttccagag cattttcgcc
    44701 atctcccagc ctatcgtaca atgtaccctt tgcaaagtaa agcgatgagt tgtttggctc
    44761 ggtttcaatt gccttatcaa tgtatacaag aatcttagta ggatcttcct gtttatcgat
    44821 atagtaggaa attagagaga ataatacgtt tgtgtttttg ggatacttta tgaaaccttc
    44881 ctctaggtat tttagacctt catcgccttt attcaggttc ttgtaggcat caaatatatt
    44941 aaagtaaaca gcaccatcat tggtaaaacc aagttcaatg gatttctggt agtatggaat
    45001 tgattcctca agtaatccgg caagttgagc tgataaacct gcatagtaat aaattgcagt
    45061 gtctttctgg ttgagaatag gaagtgtacc aatctctata acctttttga aggattcaaa
    45121 ggcacccttg taatctttta gagtatactg gtttgaacct tcgttaacgt atagaccttt
    45181 tagttttaca aggttttctt gcaatttctt ggtgctctta cccttggtgt tgatctcaat
    45241 tgctttcagg aaactttcaa gggccaaatc caaaggttta tcaataacgg gcttagtaac
    45301 ctcccagcgt tcaagttgac cgttgttaaa gtagaagtta accctttcca taacatactt
    45361 atcgagttga accccatcaa cttctacttt ttcctgctgt atgggttttc ctacaacaag
    45421 cataaatgta tttggatcca ttccaacgta tgccctgagg atttgagcat caaaaaggtc
    45481 tatcataagc tcaccccttt taatccatgt ggcatccttg gtattctttt tgggatcctg
    45541 gatttcggcg ttgctttttt caaccttttt ggcatactga tcgaaattga tttccttgct
    45601 ggtttgcgct gttaccatta cagcaacaaa gagcattgaa attaaaattg ttagtcgttt
    45661 cataagataa ttgttaaatt aagttactcg tttactgatt cgtcgttatc aatggtttct
    45721 tgttgatcaa tattttcttc atcgcttgcc tccacattgg ctatggccgc aatggaatcg
    45781 tttttcctga ggttaattag tttaacaccc tgtgctgttc tgccaataac tcgaagttct
    45841 ttggcactca atcggatggt taaaccgctc ttgttaataa tcattatatc gttattgtcg
    45901 tcaacggctt taatgcttat aagtttacct gtttttggtg taatagttat tgttttaaca
    45961 ccttttccac ctctaccggt aactcggtaa tcgtcaagtt tagaacgttt accataaccg
    46021 ttttctgcta caaccagtat atccttgttt tcattttctt caacacaaat catgccaata
    46081 atggaatcgc cctgtgctag gttcattccc ctaacgccac ttccggttct acctattgga
    46141 cgtacaagtt tttcgtggaa acgaatggct tttccttcgc gagcagctag gatgatttgc
    46201 ttgttaccat tggttaggca tgcctctata agctgatcgc cctttttgat ggtaattgcc
    46261 attactccac tctgtcgtgg acggctatac gcctctagcg atgtcttttt aattatgcca
    46321 ttccttgtgc aaagaacaat atagttatta ttcaggtact cagggtcggt taatgtttta
    46381 acattaatgt atgcacgtat tgtgtcgtcg ttctcaatgt taataaggtt ctggatagcc
    46441 ctgccctttg aagccttgtt accttcaggt atttcgtaca cttttaacca gtaacacttg
    46501 cctttttcag tgaagaaaag catggtgtta tgcatggatg ctacatagat atgctcaatg
    46561 aaatcctcat cgcgggttgt gctacctttt gagcctactc cccccctgga ttgaagcttg
    46621 tattcataca gtggagtacg cttgatataa cccatgtggg aaatggtgat aaccacttcc
    46681 tcgtcggcat aaaaatcttc ggggttgaac tcctcagcat caggcacaat ctcagtttta
    46741 cgattatcgc catacttttc cttcatctcg atcatctcat ccttgatgat ctttttctga
    46801 aggttttcat cctctagaac ctgtttgaga taagcaattt gctttagtag ttcctcatat
    46861 tcctgcttaa ccttttcacg ctcaagcccg gtaaggctcc ttaagcgcat atcgatgata
    46921 gcttgggctt gaacttcgct aagaccaaac ctttccataa gcttttcctt ggcctcgtta
    46981 ggcgaagaag cagcacgtat aaggttaatc acctcatcga tatggtcaat agctattagt
    47041 aatccttcca ggatatgggc ccgtttttga gcttcatcta gctcatattg agtacggcgc
    47101 aggataatgc tatgcctgtg cttaatgaac tgtactatta aatccttcag gttaagctgc
    47161 ttggggcgtc catccaccaa agcaatattg ttgatagcaa aagaggattg cagctgtgtg
    47221 tgcttaaaca aattgtttaa cacaacattg gcaacagcct cttttttaag gattattaca
    47281 actcgagttc cttgcctgtc ggattcatcg ttaatatagc tgataccctc aattttctta
    47341 tcgttaataa ggtcggcaat ctttttaatc atttcggcct tattcaccat atagggtatt
    47401 tccgtaacaa taatcttgga gcgcccggat tcggtttgtt caatttccgt tctggagcgg
    47461 atcaccaccc taccctttcc ggaaaggtat gcttccttaa ctccctgata accatatatt
    47521 ataccacctg ttgggaaatc gggtccttta acatatttaa gcagttcatc tatggtgatc
    47581 tcggggttat ccacagtggc acaaatggcg tcaacaatct cgcagaggtt gtggggaggc
    47641 atgttggtgg ccatccctac ggctatacct gatgtgccgt tgaccagaag aaggggtatt
    47701 ttggtaggaa gcaccgtggg ttcttccagg gtatcgtcga agtttagctt aaagtcaacg
    47761 gtattcttgt cgatatcggc aagtgtctcc tctgcaattc tggttagcct agcctccgtg
    47821 taacgcatag ctgcgggact atcgccgtca actgatccaa agttaccctg tccatcaacc
    47881 agcgggtagc gcattgccca atcctgcgcc atgcgaacca ttgcctcata aaccgaggag
    47941 tcgccatgag ggtggtactt acccaggacc tcacccacga tacgtgccga tttcttgaat
    48001 ggtttacctg cattgagccc cagatcggcc attccgaaaa gaacccttct gtgaacgggt
    48061 tttaaaccgt cacgcacatc gggtaatgct ctcgatacta tcaccgacat tgaatagtca
    48121 atgtacgatg atttcatttc ctcctcaatg ttgatctgta taatcctttc accctcagac
    48181 atataataaa agcttaaaaa gtcatttttt acttcagaaa taacgtgcta agatagctat
    48241 ttaagcgtaa aaaaaagaat ggttaatgat gttgttcatt atttattaaa aaaatactaa
    48301 atttattaac aaaagcattg ttgttacgtt tatgtctatt actttagtaa aagtttttgg
    48361 agaagtattt atggaacaaa agttttcaca aaaaatcaaa gatgttatag cttacagtcg
    48421 cgaggaggct atccggttgg gtaatacagc cattaccccc gatcatctct ttttaggtat
    48481 tcttcgtgat ggtgagggcg aagcaatcaa tattctgctt tcggagggtt taaatttaaa
    48541 cgatctaaaa aagtcgattg acagccggtt gttacaaccc gctgataata attcgggtat
    48601 tgatgagtct gaggacatta gtatttacaa atcaactgaa agggttttaa aaatcgtaat
    48661 acttgaagcc cgttcgctta aaagcgatac tatcaataca gcccatcttt tactcgcaat
    48721 tctaaaggat gaaaccagct atatcaccca gttgctacta gagcataatg ttgattacga
    48781 aacggttcgt aaacatttga ttgtggagga ttcgggtgat aaagatgact tttccggcgg
    48841 tggcagcgag gatgatgaag gcgaaggtgg tatttttggc aatatggggc gttcaggtca
    48901 atcatcctct aagtcgtccg ataccccagt attggataac tttgggattg atcttaccaa
    48961 agccgcagag gagggtcgtt tagacccaat aatcggacga gaaagggaaa tagagcgaat
    49021 agcccaaata ctgagccgcc gaaaaaagaa taatccaatc ctcattggtg agcctggcgt
    49081 tggtaaatcg gctattgccg aagggttagc cctacgaatt gttaaaaaga aagtgtcgcg
    49141 tgtgctattc aataaacggg taatcgcact cgatttagca tcaatagttg ccggcacaaa
    49201 gtatcgggga cagtttgagg agcgcatgaa agccatacta aatgagctta cccgtagccc
    49261 aaacattatt cttttcattg acgagattca tactattgta ggagcaggtg gagccaccgg
    49321 ctcgctcgat gccgccaaca tgctcaaacc tgcacttgcc cgtggcgaac tccaatgcat
    49381 tggggctact acccttgacg agtaccgcga gcacattgag aaggatggag cgttggagcg
    49441 acgcttccaa aaggtaatga ttgaacccac aacgcctgag gagacgattg aaatactcaa
    49501 caacattaag gagagatacg aggaacacca taacgtaacc tatacccccg acgccattga
    49561 ggcatgcgtg aaacttacca tgcgatacat cagcgacagg catttgcccg ataaggcaat
    49621 agatgccctg gatgaggctg gttcgcgcgt tcatatctca aacattaagg ttcccgagag
    49681 tattctcaag ctggaaaaac ttattgagga gaccaaaaag gaaaagatca aagcggttaa
    49741 gaaccagatg tttgagctag cggcaaagta tcgtgataac gaaaaaaaac ttattgaaca
    49801 gttagaagca gagcaagcgc ggtgggaaaa agagctaact aagcatcgcg aaactgtaga
    49861 tgccgaaaag gtagccgaag ttgttgccat gatgactgga gttcccgttc agcgtatagc
    49921 tcaggctgaa ggcgcacgtc tactcaaaat ggccgatgag ctgaagcgct cagttattgg
    49981 tcaggacgag gcaattgata agattgttaa ggctattcaa cgtaaccgcg ccgggctaaa
    50041 ggatccaaat cgtcctattg gttcgtttat ttttcttggg ccaaccggtg ttggaaaaac
    50101 acagcttgca aaggttctgg caaaatacct tttcgatact accgataacc ttattcgtat
    50161 cgatatgagc gagtacatgg agaaattctc cgtgtcgcga cttgttggtg cgcctccggg
    50221 atacataggt tacgaagagg gagggcagct caccgagaaa gtacgtcgaa aaccctactc
    50281 ggttgttttg ctcgatgaag ttgaaaaagc acaccccgat gtttttcaca ttctactcca
    50341 ggttttggac gaaggccagc taaccgacag cttaggacgt agggttgatt ttaagaatac
    50401 cataataata atgacctcca acattggaac ccgtgatttg aaggagtttg gacaaggtgt
    50461 aggttttgcc acaaaagcca agtcggaggc aaaaagcgat tacgagaaaa gtataatcca
    50521 aaaggcgctg aaaaaagcct ttgcccccga attcttaaac cgtattgatg acattgttat
    50581 gttcaactcg cttaccaaga gcgatataca caaaatcatc gatattgagc ttaaaggatt
    50641 attcgacagg gttgctggtt taggctatac cattaagatt tcgactgcag ccaaggattt
    50701 catcacggaa aagggatatg atgttcagta tggcgcgcgt ccactaaaac gagccattca
    50761 aaagtatctg gaagatcctc tagctgaagc cttaataaag agcgatgcaa aacctggtgc
    50821 agtattcctg gttggctata gtaaaaagaa tgacgaaatt accattcgaa cagcaaaagc
    50881 tgaagaggag gttaccaacg ccgatgaaga gtaataagtt gtaatacaat gagaaagaga
    50941 cgcacaacaa aaggtgtgtc tctttttttt gaacattccc ctagcaaagt tgttatatta
    51001 gtagcaaact aaccaaatag aattatgaaa acagtaacat ttggcggaaa tcccgtaacc
    51061 ctgacaggcg aacaggttaa ggtaggcgat aaagctttag aatttacagc tcttgataac
    51121 tctctaaagc ctgtaaaaat gagcgatttt aagggaaaag taaaactaat atccattttc
    51181 ccatccattg acaccagcgt atgttcggtt cagaaccaca agttcaacaa gaccgcatcg
    51241 gaatttggcg ataaggttgt atttatagcg atttcgaacg atttgccttt tgccctaaag
    51301 cgtttttgtg gagctgaggg aattaccaac ttagttaccc tttcggatca tagagaccta
    51361 gagtttggta caaactatgg ttttctgata aaggaattaa ggctacttgc ccgtggtgtt
    51421 gtgataatag ataaggatga taccattaaa tacgtggagt atgtacctga aattgcccaa
    51481 gaaccaaact acgatgctgc aattaaagta ttaaaggaat tagttaagta gcatgatgca
    51541 ttaaattgaa ataaaggtcg ttaacaacga cctttatttt ttatataagc tcaccgatta
    51601 aatcgtattc ctgggcctct acaatacgta ctgtatgaaa ctcgccgggt tttacattaa
    51661 tagcgttaag ctttaccaga acctcctgat ccacctcggg tgaatcgaac tccgttcggc
    51721 aaacagcgta atggtcttca attgcatcaa ttacaacctt atacgtatga ccaactctca
    51781 gctggtttaa ctcaaaagat accctttgtt gaacctccat cagcttttca actctacgtg
    51841 ttttttcttc atcgggtatg gagtcggtaa agttttgtgc agcataggtt ccttcctcct
    51901 cagagtatgc aaaaacacct accctgtcga accggtaatc cttaacaaaa tcaatcagtt
    51961 cattgaaagc ctcaggggtt tcccctgggt ggccaaccag aaaagtggtt cggataacaa
    52021 tttcggggat attctttctt atcttctcaa tgaaatcaat ggtttgttct cggcttatcc
    52081 ccctacgcat cattttaagt acctgagtat ttatatgctg taatggaata tcgatatact
    52141 tgcaaatttt cgggttcgac tttaggacat caattaggtc atcgggaaaa ccagatgggt
    52201 atgcatagtg aagcctaatc cattcaaaac cgtcaatctg gctgaggcta ttgagtagct
    52261 gagcaattcg gcgttcacca tatatatcaa atccgtaata ggtagtgtcc tgtgcaatta
    52321 caattagttc cttaacgcct tgtttggcga gttcttttgc ctcgttaacc aatgattcaa
    52381 tggggcggga aacatgctta cctctaataa ggggtattgc acagaatgaa catccccagt
    52441 tgcaaccctc ggatattttc aggtaagcgt aatgccttgg ggttgaaagc cagcgtttat
    52501 tcagctcggc ttcgttatac ttcacattga gctcagcaac aatttgtggt aaatcggaaa
    52561 ccccaaaata tccatctact tcggggattt cagtttccag ttcattgcgg tagcgttgcg
    52621 aaaggcatcc cataacataa accttgtcga tcagccccct atttttggca tcgacaaagc
    52681 caagtatagt ttcaaccgat tcttccttag catcgccaat aaaaccgcaa gtgttaatca
    52741 caacaaccct tgccgatgga tcgtttgaat tgtgaacaat tttccatccc cctctatcga
    52801 gctgatgcat aagcacctcg gagtcaacaa cattttttga gcaccccaag gtaacaacat
    52861 tgattttttt cgtcatgttt ggctaaacgt tacgaaatag cgattcaaca aacgcaaccc
    52921 tgtcaaatat ctgcaaatca gtcatttttt cacccactcc gatatacttc acgggtatct
    52981 tgaattgatc ggaaatgcca agcacaacac ccccttttgc cgtaccgtcg agcttggtga
    53041 gagcaatggc tgtaacatcg gttgccgaag tgaactcgcg tgcctgtgca aaagcatttt
    53101 gacctgttga accatccaga acgagcaata cctcatgtgg tgcatcggga ataacctttt
    53161 gcattaccct acgtattttg gaaagctcgt tcattaaatt gactttattg tgaagacgac
    53221 cagcggtatc gataattact acgtcggtgt cgttagattt ggcagagttt agggtgtcga
    53281 aagcaacggc agctggatcg gctcccattt gctgcttaac aatgggaaca tctaccctat
    53341 tagcccaaat ggtaagctgc tcaacggctg ccgctctgaa ggtatcggca gcacccaaca
    53401 ataccctttt cccctggttc ttaaacatgt atgccagttt gccaatagta gtagtttttc
    53461 caaccccgtt aacgccaact accataatta catagggttt aggaatttca ccactattat
    53521 taaggataga attttcggaa ttctcggcaa gtaacgatag tatttcggtt ttaagtatag
    53581 tatttaattc tgctgtattc aggtactttt cggcagcagc acgcttttgg atgcgttcaa
    53641 taatgcggag agtggtttca acgccaacat cggcggagat aagtccctcc tcaagctcgt
    53701 ccaagacctc gtcgtcaacc ctggacttac cggctacagc ccttgaaatt cttttgaaaa
    53761 ggttctcctt tgttttggcc aaaccctggt caagggaact ggagttatta ttgtccttac
    53821 tatcgggatt aaaaatgtta agaataccca tatacaatat tattttcggg cgctaaaata
    53881 acaataaaag cttttcgaat aaaccgaaaa gcttttattt gtaagatata atgcagaaaa
    53941 ctatttctgt ttgaagaaat cggccgattc ggatttatcc attgtttctt ccttgaacat
    54001 gtatgcgccc gttttgggtg aacgcaccat tttaatgcac ttcacacgat ctttcgtggc
    54061 tttttgaagc gaagcaacta ctttctttgc catatctaaa agtgttattt aatttcacga
    54121 tgaagtgtaa ccttcttgag aatcgggttg tacttacgac gctcaagcct atcgggtgta
    54181 ttcttcttat tcttagtggt aatatacctt gaagtaccag gtaaaccact ttccttatgt
    54241 tcggtacatt ccagaataac ctgaactcta ttacctttct ttgccattgc tatacttttt
    54301 taaaaattaa atgtgacctt tcttagcaaa tttcttcagg gcattatcta aaccaacctt
    54361 atcgatggtt ctcatagctg aagtgctaag cctaagggta acccaacggt tttcactttc
    54421 aaggaacaac cttttggttt taaggttagg caggaatctg cgtttggttc ttctttttga
    54481 atgcgaaacg ttgtttccca ccattactct ttttccggtt aactgacaaa ctcttgacat
    54541 cgctgtatat tttaccgttt ttactttttt cgaaacagga cgcaaattac gtcattattt
    54601 atgaattatt caaatatttt gataaaaaaa attgctgaaa attagcttaa tttagatacc
    54661 ttctcattta atgatgaaat tacaattacc atatttatat tatatacatt tatttacgaa
    54721 gaatatttaa actgatacac taatccttat ccataagttt tagcaattgg tgcaaagccg
    54781 tatatgctgc gcgttgaata ttaacctccc tgaggtttcc aaaagtatag agttctacca
    54841 gtcgcgtttc ccttgatgca acaccaatcc aggtggtgcc tacaggctta ccggcggttg
    54901 ctcctgttgg tccggcgatg ccggacactg caatggcata ctcactctgg aaaactctta
    54961 gtgtaccatc gagcatggcc tcaactactg gttggctaac tgcgccatga cttatgagca
    55021 aatcatggct tacccctagc acgttttgtt taatctcgtt tgagtatgct acaatacctc
    55081 ccttatatat atcggagctg ccgggcagct gagttattaa atgcgaaata tagccacctg
    55141 tacagctttc ggccactgct agggttgcat ttttttccct aagtctttta gacactaccg
    55201 agggaagtgt ttcgtcattg aaaccaaaaa tattttcacc aatcaaactc ttaagttcat
    55261 ttactgccat atccaacaag ttgcttagcc tacccttatt ttcaccagtt gcagtaagac
    55321 gtaacctgat tcccgaaggg cttggcaggt aggctagcgc aatacttcta tcagctaaat
    55381 atatttcaaa gtcttttaac ttatcggaaa ggaacgattc agggattcca aaggtgttga
    55441 cggttacccg aactattgcg ggaagggtaa aattttgctc aataaatggc cgaaccccct
    55501 cagtaaacat attcttcatt tcaaatggaa ctcccgggag cgaaatcaga aggcaattgc
    55561 ctttcctgaa tatcattcca ggagccgttc catgatagtt gagaattggt gtgcacgaag
    55621 agggaattaa tgcttggttt ttattggtct cagttagagg aagaccgcgt ttgtgaaaaa
    55681 tctgttcaat taggcttagg gagggtgcat ggagcataag ctgaccacca aatagctcac
    55741 atagcgttgg ctttgtccgg tcatcgctag tagggcctaa cccgcctgtg gaaataacaa
    55801 gatccgactg gcccattagt tcgttaacag cttcggcaat tgcttgtgag ctatcggaaa
    55861 tgctaaggat tttggttacc tcaaatccaa gctcggtaag gctatcggcc aaccaggccg
    55921 aattggtatc gataacctga ccgataagta actcatcgcc aatggttagg attgatgctc
    55981 tcatttgctt gcaccactaa atttaaaaag gacaggttgc cctgtcctac cagagcctct
    56041 cacgggactt gaacccgcga cctgcggttt acgaaaccgc cgctctaacc aactgagcta
    56101 aagaggcatt attaccgctg caaaaatatc aattagataa caattcgcaa aacaggctat
    56161 aggatagaaa taaaaataat ttataattaa ttgatataat gaattttgcc actttctact
    56221 ttacgagtaa ttatagaata ttaagcttac ttttggggtt tattgttctc tattcggtgc
    56281 tgcaaaataa ccttatactc ctttagaaac ttaaggtgag ggtatttaga ttttgcaaag
    56341 tcgagccatt caatggatag ctggaatttg cccagcatct cgttggctag ggccatattg
    56401 aatgctgctt gtgcagccaa ctccttactg gttgatgaag ataacctttg ccaaaccgcg
    56461 gcggcatcta accagtttcc atgcatggca agttggtatg ctctgctcat ctccttgtta
    56521 gtgctgggaa catatattgc tctgctgtcc ttttgccatt gcggagcaat aagctgtgcg
    56581 aattgctcgc cggcatcggc tccagcaaat gcacaagcct cgaatatacc cggaagctgg
    56641 ttgccaggtg taacctcaat ccaatcgttt ttttcccata aaagggtatc ggtaagtagc
    56701 tggcctaaat taatctttct tgtttcaaca ttgtaaatgc gccaataggt atagcttgat
    56761 acctgaatga atccgtaata ttccttaaaa tcgccacgcc acaaagggta tgtatcatag
    56821 tatggacgta cttttaagta ctcaagcgag acaaccattt tactattgta tacattgctg
    56881 aggctatcga ttacttgcca ggaaagggga agaacaaccc ttgaagaatc gttacggtaa
    56941 taaatatgtt taaaaagggt tataccggag taccatggag aagtttttaa taggcttatg
    57001 agggcatcgg ttgcttcatt tgaagcaacg ctatccaaag cccaatcaag ttcccgaatg
    57061 ggagtatcgg tatttcgtag gtgaagattt tcaaccacta gcaggttttc acccggaaca
    57121 acaatcgatt tttggggtga atcgtaaaac tcaatatcaa tatacctata tgatatacaa
    57181 gatgttaatg agaaaagtaa tactaaccat ttaaactttt ttatccacat aaacttttaa
    57241 tagggttttg gcatttcata aaataaaaaa gttgaaaaca agcagcttaa gtattagtat
    57301 gcctgctttc aacttaaatt ataatgctac aagtactagg tattcattgc accacaaaca
    57361 ttaataacct gtccgctaac gtacgacgat agatcggaac ccaggaatac acaaacattg
    57421 gctacatcgt cgggggtacc accacgctga aggggtattt tctttatcca atcctcgcga
    57481 acttcatcgg gtaacttgtg ggtcatttcg gtgataatga atccgggagc aattgcgttg
    57541 caacggatgt tacgtgagcc cagctcgcgg gcaacagctt tggtaaatcc aattattcca
    57601 gcctttgacg ctgcatagtt ggcctgtccg gcattacctg aaactccaac caccgagctc
    57661 atgttaataa ttgaaccgtt tttctgctta agcatatact tttgaacggc cttggtaagg
    57721 ttaaaaaccg actttaggtt aaccttaatc actaaatccc attgctcctc ggtcatacgc
    57781 ataagcaatg tatcgcgagt gataccggca ttgttgacca ggatatcgat ttggccaaaa
    57841 tctttggcta tttcatcaac aattttctgg gaattatcgt aatcgctagc atcggaagcg
    57901 tatcctttgg cttttacgcc taatgcggtt agctcctgct caagcgattt catgttgtca
    57961 tcgtaagcaa tgtcggtaaa agctacattt gctccatgtt gagcaaaggc aattgcaatg
    58021 gcgcgcccta taccccgagc cgcacctgta accaagccag tctttccttc aagtaacttc
    58081 ataggtttaa tttttgaatg attttcaaac gcaaatgtaa taaatattgg taattaaact
    58141 tctgttttaa gtatcaaagc tcacttattg ctcctattat tgcttgttta tttcaaaagt
    58201 gctatattca cacattaaaa gttgattgtt tttcttcaaa aaacatttac catgaaggtg
    58261 aaaattcata ttagagtaaa gttgattatc gctgtactta cggttacagc agttgttttt
    58321 tgggcaagca ttgctttttt aaacaaaagg gttgataggt atactcggac aaacatttat
    58381 gaatacattg atgccacgtc aaaaacctac accacccaaa ttaagggtga atttgataag
    58441 cagtttgcct tagcaagaac cataacctat agcttttatg accttgaaca acgaaatccc
    58501 gaagatgtta agcaaaatac tttaaggcta ctaaaaggaa ttgcagaaaa tacccctgat
    58561 ttactgtcgg tttgggcaag ctgggaactt tgggccttag ataggaaatg ggataaacct
    58621 tacggcagag ataggtacac ctacctgagg gaaaacaatg aacttaaaca aatatttgaa
    58681 agattaaacg agaccggtga taacccaagt agtttgtact ataaacttaa aaccgaaaaa
    58741 aaagaagcac tggttgatcc atactggtac atctatgccg gaagtcaaaa aagggtactg
    58801 gagaccagcg tatgcgtgcc gttggtagtc aacaacagat ttgctgcgct ctttggcttt
    58861 gacattgagc tctcagttta tcaggaaatg ataaagaatc taaaacccta cgaagggagt
    58921 tacgccattc taatttcgaa taacggtacc attgttgcgc atcccgattc cacattgctt
    58981 ggtgcactgg ccgatacggt tttgactaac ggaaaacaaa taattaacag cgtaattacc
    59041 aatgggaaat actcagaaac agtgttagag ggaaaaagta agtactacct tacctttaac
    59101 tcttttactg caggtcaagc tgctgaaagg tggagccttg gtatattcgt acccgagaaa
    59161 gtaattaatg aacaaataag caccataaca agccaggtta ggattgtaat ggtaatagga
    59221 ttaatagtgc tagcaattgt gctttggatt attgcctaca gcattacaag cccattagta
    59281 agagcaacaa aagttctagg tgaactctcc cttggtaaga ttgacccagc caaaaagatt
    59341 gagataaaga ctggcgatga gattgaggat attgcaagtt caattaacat tttaattgac
    59401 agtttgttta aaacctcgca gtttgccagg gaaataggta aaggtaattt acaagcaaaa
    59461 tactcaaaac tatccgatga cgacgtttta ggtgatgcgc tacttgaaat gcgtaagagc
    59521 ctggagtatg ctaaaaagat tgatgaggaa aggaaagctg aggaaaacaa gaatagatgg
    59581 tacaacgagg gcatggctaa gtttgctgaa atattgaggc acaataacag caacctaaac
    59641 gactttgctt acgaaaccat tcgaaaccta acaaagtatg ttgatgccac aattggtgct
    59701 gtatttctta tcaataatga taatccaaat gatatcttcc ttgagttgat ggcaacctat
    59761 gcctacgaac gccgaaaata cgaggagaaa gtcattagaa tgggtgaagg ccttgttgga
    59821 agatgcgcac aggaagcaga aaccatttac atgactgaaa tacctaacgg gtatataaaa
    59881 attgcctctg ggcttgggca ggatgaaccc accgaattac tccttgtccc tatgaagatt
    59941 aacgatgaag ttcacggggt tattgagatt gcctcgtttg agcctattga ggattataaa
    60001 attaaattta ttgaaaagat aggcgaaagt tttgctgcaa ccatatcgag tgttaaaata
    60061 aacattagga ctgcaaaatt acttgaagaa tcaaggataa aatcggagga gctcgcatcg
    60121 caggaggaag agatgcgaca aaacatggaa gagcttcagg caacccagga ggaatcggct
    60181 cgcaagtcgg ccgagatgga aagcctaatc aatgctttgc attcttccag ctacgttatt
    60241 gagtacgatt tgaatggcaa cattatcact gtaaacgaag cctaccttaa actaactggg
    60301 caaaccgaga aagatattgt tggaactcac catgccgaca acctggagat gacctccgaa
    60361 cagaaaaatg cctaccaaaa gttctggcag gacctacgca atggaatgat taaaaaggaa
    60421 acatctaagg ttaagattgg cggtaaaacc tatacattca ttgagaccta ctcacctatt
    60481 cttgatgaaa acagaagggt tgtgaagatt ctgaaaatag ctcacaacat aaccgatttt
    60541 attgatgaaa atccagaggg gaaaacaaag aaaaaataaa aaaaaggccg gggtaatccc
    60601 ggcc
//
LOCUS       DAOSAL010000007        53636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_478_length_53636_cov_49.956925, whole genome shotgun sequence.
ACCESSION   DAOSAL010000007 DAOSAL010000000
VERSION     DAOSAL010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 53636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 53636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53636
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_478_length_53636_cov_49.956925"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="PK049_02005"
     CDS             complement(<1..230)
                     /locus_tag="PK049_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60359.1"
                     /translation="MIVYADIKEFYLIWQYHFIQKISMLNLFKKHRDDELEKRIDCLR
                     SILLFKEVDHSTLRKIASVLESCEVKKDERLF"
     gene            276..2567
                     /locus_tag="PK049_02010"
     CDS             276..2567
                     /locus_tag="PK049_02010"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOK60360.1"
                     /translation="MRLKLTIRQKILYYILGSAFIIFSIVFYFISSSSRRLAYEQSIK
                     LTNSYARQYALNIESWINQDFAVTRTLAAAFMEYKQMPFDQWQKLIYPMYNRVILTTP
                     HIDAYWDSWELSNLDPNWTLPYGRYFYIVYKQDGVYKTKAEMRSLTGDPPTYDSMKSA
                     AMEIVVEPYISELQQGQMMTTLSSPLLENGKFIGLIGADLILTRFQQLVNNIKPYPNS
                     YAFLLSNKGVFIAHPDSAIFKKNITEVLPEFNNQHNILARVQKGKAFSFIHQDSKGER
                     IYYTFEPIRIGNTQTPWSIGIAAPVTDIMAQTNRSYNTSLLIALIGLLVIIFMGYFVS
                     NNITLPIKKITQILNEFAKGKIVSSIDISTSSNDEISQMVEAVSASLAGVNSKTTFAR
                     EIGAGNLNVNLSLLSDEDQLGKSLIEMRDNLRRARDEEEKRKIEEEKKRWTNEGMAKF
                     GEILRQNNNNLSLLGQELIKNLVWYLNAQVGGLFVLSENKETGDKTFDMVAMFAYDRN
                     RVMQRSYHFAEGLIGACAAERDAIILKEVPQDYIEITSGLGGINPNYIIVVPLIFEEE
                     VMGVIEIASLQELKEHEVQFLKDIAKSIASTLHTVKVNTLTAELLMKSKEQAEMMAAQ
                     EEEMRQNMEELQATQEEAARKTLELEGLVNALNAAAFVMEYDTKGYVININDSYLNFL
                     GANRDDIIGLHHSENIILSDTEKASYDKMWSDILRGNIRKHKTRIRVNGQEHLLLETY
                     TPIFDHGGQLLKILKIATDITNI"
     gene            complement(2564..3370)
                     /locus_tag="PK049_02015"
     CDS             complement(2564..3370)
                     /locus_tag="PK049_02015"
                     /inference="COORDINATES: protein motif:HMM:NF022430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2797 domain-containing protein"
                     /protein_id="HOK60361.1"
                     /translation="MKGVLTKMGFTNTGNDVSISPEYFLDLDSGRVNVSELIGKGIGL
                     KYLNINRCIHCGRETKKLFGQGFCYPCFIKVPEAEECVLRPELCRAHEGIARDLEFAK
                     ANCLVQHYVYLAWTGGLKVGVTRYHQIPTRWVDQGATFAVKICHTPNRYTAGLAEVEL
                     KKIFADKTAWQRMLTDSSELPPDFLTHKAKALEFINGKNLSYIPESDVVYRVSYPVIQ
                     LSDKVRSVNLEKVNSIEGKLVGIKGQYLIFESGIVINIRNHSGYLVDITF"
     gene            3645..4178
                     /locus_tag="PK049_02020"
     CDS             3645..4178
                     /locus_tag="PK049_02020"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOK60362.1"
                     /translation="MKRTISVSLNGKAYMLEDDAYHALDRYLKGLEAHFANDPNKKEI
                     VGDIESRIAEHFDEFIKVPGQVVNLEEVNRVVHLMGNINDFDPTNTKASSENDRVYPK
                     LYRDVEDRILGGICTGMGHYWKVDPVLFRLVFFLLTLWGGLGVLIYIVLWVVVPPAIT
                     PTQKLEMKGEKINVNNM"
     gene            4252..4593
                     /locus_tag="PK049_02025"
     CDS             4252..4593
                     /locus_tag="PK049_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOK60363.1"
                     /translation="MNQEIENAKAQMRKGVLELCVLTVLSRGDAYANDLINQLKQAKM
                     IVVEGTIYPLLTRQKNAGLLTYRWEESPQGPPRKYYSLTEEGKEYLAELLNSWDELVE
                     MVSAIRNQKND"
     gene            4636..6198
                     /locus_tag="PK049_02030"
     CDS             4636..6198
                     /locus_tag="PK049_02030"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOK60364.1"
                     /translation="MNTTVNVSIGGYSFTLEENAYNTLQEYLNTVKVKLGNDKVAQET
                     VNDIEFRIAELLTEKITLGQVVTNEMVTPIIEQIGKPEDIGENNDSTSNKQYRPKRLY
                     RNPDDSIIAGVCGGLAAYFSVDPLVFRILFFALLFLKGFGLLLYILLWIAMPKAKTPF
                     QKMEMMGDTENIDEISRRVRKEISNASDNLKKSKAGNTVNKLINLFGQVMLYLLKAML
                     VIVKVISITIGVLLIVSMLLIFIALVGAVFFASYSPDFSGVIGGVGMTLNEIIASVLD
                     ISSSYWITIPLFLTLAIPIVALIYAGIRILFRFRAKDGTIGIIATVIWAAAVVTLSLT
                     FFFQMRGLSVSEKVVKELEISPVNSNSKIVLCKSYNYMTDSTSSIEEKVQIFDWKIVT
                     LKGKHQLAGSPTLIIDKGFEAKPRFEVIRKARGVNSYTAADNAKDIIYSLTLNDTLAI
                     LDPLYIIPEKTKWKNQSVLIKLYLPEGYGVYLDETLTEMLEKYQPYSNYWPDEMVGKT
                     WIMTANGLRIAR"
     gene            6274..6347
                     /locus_tag="PK049_02035"
     tRNA            6274..6347
                     /locus_tag="PK049_02035"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6306..6308,aa:Gln,seq:ttg)
     gene            complement(6376..7905)
                     /locus_tag="PK049_02040"
     CDS             complement(6376..7905)
                     /locus_tag="PK049_02040"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOK60365.1"
                     /translation="MINIKEIVLNFLHYTEGDSLIFTQLSFWVFFGVLLAGYTFVYNR
                     PVIRSAYLLVFSLFFYFKSSGLFFSLLLFSTLVDYTIGLAIAKSGSKINRKLLVALSV
                     LVNLLVLSYFKYAYFIVDLLNNLLGINLTVVNHLALWGNALLNTKFDTTTIVLPVGIS
                     FYTFQTISYTVDVYRGKVAPVRNIIDFAFYVSFFPQLVAGPIVRAAEFIPQIYRRYEL
                     SKNEFGHALFLVLAGLVKKMVISDYLSVNFVDRVFSNPLGYTGFENLLATYGYAMQIY
                     CDFSGYTDIAIGVALLLGFKLPINFNSPYKATSLTDFWHRWHISLSLWLRDYLYIPLG
                     GNRKGRIRTYINLLITMLLGGLWHGAHLKFVVWGGIHGVGLAIEKLLSNLRLVNINSE
                     RHSTRLIWGIITFNVVCLSWIFFRANDFNAATDMLSQIFTSFGTKTIPDIALAFSDVF
                     LVLALGFIVHLLPYNAKEYLRGRFIAMPLSLKLFAALLVVVVLIQFQTMELKPFIYFR
                     F"
     gene            complement(7915..9102)
                     /locus_tag="PK049_02045"
     CDS             complement(7915..9102)
                     /locus_tag="PK049_02045"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOK60366.1"
                     /translation="MNRLVLHITGIAIFLILTLLPYQVSLLVPTKSPAILLFNHSANT
                     IKNFFGQKIVVKPSKPLTIVHIGDSHVQAGILSRTVKMFLAREFHSQYVSPGLVFPYS
                     VMGSNNPVEYTSYHTGRWEYQKVNGAKAPVDAGIFGVAAKTTDSLATLSFKVKPNALF
                     KTQVNRVGVFYQSASSYLTPILVEPKNVNPILHENFVEFELESEVDSVVIGFRAKNDF
                     ECTVYGVNLWNTKSKFSLSSVGLNGATVHGFSLAKQLLPNIKLIKPDIVIVSLGTNDA
                     YSNLFDSLNFARDQENLINTIKSALPNPFIILTTPNDHLLNRKYMNIRVQVASNVIKE
                     ISTKHQLPVWDFYSLMGGKGSIQGWIRYGFAAPDGVHLTAKGYQLQGELFYHALYNSG
                     FIE"
     gene            complement(9095..10564)
                     /locus_tag="PK049_02050"
     CDS             complement(9095..10564)
                     /locus_tag="PK049_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60367.1"
                     /translation="MNKPYHTLGFLLLVAILYTLLSIIVPGSTFRVDDFLFRFPKPFE
                     QLAGDNIKIASSKTDSIQPDVNSKIETIAEESIPNLDSLYVEPVRASSDSAIAPPKSI
                     VTAGTLHLEMPQGINALGSFISAIYRNSDEVTTVRVLHYGDSQIEGDRITSQLRAALQ
                     EKFGGKGLGLIPIWNTEYLPYGFRVSLSEHVNVNSTVKAKPTQKPDCVLGSIAEVAPS
                     NLYREIVSIKVNSKQSFNTLSIISGNSDSSYAFKIIVDGTDTLETQVVPYSKVKLVKV
                     NLPSRFERISIQLRNPSTVKLYGLLAESGSGVSVCNIALRGTSGNFFNHFDSEVASEI
                     YRILNVRLVIMQFGINVVPSNLSSYAYYQKLMEQQIKAVKCFAPQADILVVGVTDMAN
                     RFDGELRSNPSVGKVLQAQRYAALGNGALFWDGYSAMGGEGSIIEWVNSKPPLATKDY
                     THFTNYGAKRFGQKMANALLMALGNNNQTTAFPDSVSHE"
     gene            10690..11037
                     /locus_tag="PK049_02055"
     CDS             10690..11037
                     /locus_tag="PK049_02055"
                     /inference="COORDINATES: protein motif:HMM:NF015893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HOK60368.1"
                     /translation="MAMIRVLHNPRCATSRKGLEYLNSKNINFEVINYLSTGLSRDIV
                     KEILLKSNLKPIELVRTQEEYYKKFLKGKNFNDEEWIEILTQNPKLLKRPVVIGKYKA
                     VIGIPVENIDKVL"
     gene            11050..11901
                     /locus_tag="PK049_02060"
     CDS             11050..11901
                     /locus_tag="PK049_02060"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOK60369.1"
                     /translation="MKLGNLILAALTPLLFSPTAFSQNADLVSMENEILKIVNEHRKS
                     IGKSELVFNDYIRKEAYIHSQNMASGKVTFSHKGFDERFNRLSGFLEITTGAENIANG
                     PLNAKHIVNGWLASPPHRKNIEDDFNLTGIGIAKAPNGSYFYTQIFVKSAEKPKVVPR
                     EYEAELLQLINNHRKGLGLPAIKNDEGIHTEALGYSQQMATGKVPIGPPSFDNPIKSL
                     MRRYKASKMVELIGYDYLKPKELFDAWMSSTSQRDIIEGNFNLTGIGVYQSVNGKVFV
                     TQVFLLQ"
     gene            11922..13541
                     /locus_tag="PK049_02065"
     CDS             11922..13541
                     /locus_tag="PK049_02065"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOK60370.1"
                     /translation="MATPEFFYQERFPLEKDDTDYYLLTKDFVSSAKFEGEEILKVDP
                     EGLAFLARTAMHDISFFYRAEHVKQIAAILEDPEASKNDKFVALVLLRNAEIAAKGVL
                     PLCQDTGTATVFAKKGQRVWTGVNDYEALSRGIYKTYTEENLRYSQTVPLDMYTEKNS
                     GTNLPAQIDIYSTHGNEYEFLFIAKGGGSANKSMLFQETKALLNPEKLEAFLVEKIKN
                     LGTAACPPYHIAIVIGGTSAEDTLKTVKLASAKYLDNLPTSGNIHGRAFRDKELEEKI
                     LKATNSIGIGAQFGGKYLALDIRIIRLPRHGASCPVGIGVSCIADRNIKAKINSQGIW
                     IEKLEKNPGKYIPDQWRKPEENDAVGIDLNQPMQAILSELTKYPVGTRLSLTGPIIVA
                     RDIAHAKLKERLDKGEGLPEYVKNHPIYYAGPAKTPAGMPSGSFGPTTAGRMDSYVET
                     FQSQGASLVMIAKGNRSQAVTDACKKYGGFYLGSIGGPAAILAQENIKKVELIEFPEL
                     GMEAVYRIEVVDFPAFILVDDKGNDFYKQIK"
     gene            complement(13608..14660)
                     /gene="queA"
                     /locus_tag="PK049_02070"
     CDS             complement(13608..14660)
                     /gene="queA"
                     /locus_tag="PK049_02070"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOK60371.1"
                     /translation="MKLSQFKYNLPKDLIAKFPVENRDESRLMVLNRKTGKIEHRIFK
                     DIIEYLNEHDVLVFNNTKVFPARLYGNKEKTGAEIEVFLLRELNREQRLWDVLVDPAR
                     KIRIGNKLYFGEDDVLVAEVIDNTTSRGRTLRFLFDGSYEEFKETLYRLGEMPVPKHI
                     LQREAVPEDRERYQTVYAKHEGAVAAPTAGLHFSKHLLKRLEIKGINFAEITLHVGLG
                     NFRTVDVEDLTKHKMDSEQIWISDDAANIINKAKREQHNVCAVGTTVLRTLESSVTTD
                     GFLKPYSGWINKFIFPPYEVAVPNMLVTNFHLPLSTLLMMVCAFGGFDQVMDAYKVAI
                     KEKYRFGTYGDAMLII"
     gene            complement(14795..15508)
                     /gene="truB"
                     /locus_tag="PK049_02075"
     CDS             complement(14795..15508)
                     /gene="truB"
                     /locus_tag="PK049_02075"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008627911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOK60372.1"
                     /translation="MEVNRFESIKEGTILLIDKPYDWTSFDVVGKLRSLIKKYTGDKS
                     TKIGHAGTLDPLATGLLVICTGKATKRVMELTADNKEYIAKIKLGETTPSFDLETDVD
                     AVYPFDHVNRELVETVLRTFSGWQLQEPPAFSAKYVNGKRAYEYARKGVDPKLEPVPI
                     EIHSIELIGFDLPFLTIKIVCSKGTYIRALARDIGKALSTGAHLVELRRTRSGIFSIE
                     QALSIAEVEKKLSDLGNQS"
     gene            complement(15512..16288)
                     /locus_tag="PK049_02080"
     CDS             complement(15512..16288)
                     /locus_tag="PK049_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOK60373.1"
                     /translation="MTIFEAIIIGIIQGLTEFLPVSSSGHIELSKALLGVQLENNLEF
                     SIAVHVATVLSTLLVFRKEIIDLFSGFFSLKLNSQTHYVFKLIISAVPVGIIGVLFKD
                     QIETLFEGNLLVVGSMLLVTAILLALTRIVKPKNTKPVGYFDSVVIGLAQAVAVMPGL
                     SRSGATISTGLLLGKDRDEVARFSFLMVIIPVLGAAFLDIIKGDISNTITAPVVAGFV
                     AAFVSGFLACSFMLRIVRKGNLYWFALYCALVGIVALFLA"
     gene            complement(16315..16584)
                     /locus_tag="PK049_02085"
     CDS             complement(16315..16584)
                     /locus_tag="PK049_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HOK60374.1"
                     /translation="MSAKNTPKKEIQEKENLFPLSWMNYKLMIIGFAIIVLGFILMAG
                     GGSTDPNVFNPDIFSFRRITLAPVVVLFGFAFVGYAIMKREKKDS"
     gene            complement(16604..17482)
                     /locus_tag="PK049_02090"
     CDS             complement(16604..17482)
                     /locus_tag="PK049_02090"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HOK60375.1"
                     /translation="MANSVNRATKGRLRSSYISSIISISLVLFTLGLLGVLVISAKKL
                     SAYVKENIGFDIYLNEGVTDADALYLKKQLDASIYIKSTQYFTQQDAAKMMANELGED
                     FIAYLGYNPLNAYIEARLKADYANNDSIARIEGWLKRFPQVKEVDYQRSLINLVNDNV
                     SRISLVILTFAGLMLFISLVLINNTIRLSVYSKRFIINTMKLVGASWGFIRRPFLLKS
                     ILHALYASFIAIALLIGLLYAINKEVADLLAILDPPSIVLVFAAITAIGIVINFLATL
                     MAVNKFLRSSTDDLYY"
     gene            complement(17483..19897)
                     /locus_tag="PK049_02095"
     CDS             complement(17483..19897)
                     /locus_tag="PK049_02095"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HOK60376.1"
                     /translation="MNSKFSKIKPHIVALLLFMAIPLAYFYPQLQGKRLAQHDIAMWK
                     GSAKEILDYKAKTGEESLWTNSMFGGMPAYLISANYKGNLLKHVDSFLNVVGRPASYI
                     FLTMLGFYILLLVFGVNPWLAIVGSIAYGLSTYFLIVIGAGHNAKIHAIAYVAPTIAG
                     MILALRGQLFAGLAVFGLAFGLNLNAGHPQITYYGALVMASVYLYYFVEAFKAKALRM
                     FTKATGILLLAAVLAFGANFTYLWFTNDYGKDTIRGKSELTHDRDNRTSGLDKDYATQ
                     WSYGIAETFNLLIPNLMGGSSTMDVGEKSETFRFLRQNGVPYGQASSIAQNLPTYWGP
                     QPSTSGPVYLGAIVVFLFVFGMFILKGRFKWFLFGITVLAIMLSWGKNFNFLTDLFLN
                     YFPFYNKFRTVSMILVIVELTIPLLGILALKEIVDGKVTKDDFWKSFKWSVGLVGGIC
                     LLFLLLGKGMLSFSGSIDNQLGWPKEIVEVMRKDRQALMWNDAFRSLFFVLAGAVTIA
                     LYYMKKLKPTLFFALLGLLITVDLWVVNKRFMDNKNFELPQQVEAPFTPTDADKQILA
                     DNDPNFRVFNLTVSPFNDASTSYFHKSIGGYHGAKLRRYQDLIDFHLSKGNIAVFNML
                     NTKYIIQPGKEGPVAVYNPDALGHAWFVDSVKVVNIADEEIEALNGFNPKSIAVVDKR
                     FEQYLRGFEQGKSGQGKIELTEYRPNRLKYRSDSPVSQLAVFSEIYYPKGWQAYLDGT
                     PADHFRVNYVLRAMQIPAGNHEIEFVFDPPMWKKGMYIDLASSLLLILTFVGWVSFSL
                     YKTFKE"
     gene            complement(19914..21464)
                     /locus_tag="PK049_02100"
     CDS             complement(19914..21464)
                     /locus_tag="PK049_02100"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019131137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOK60377.1"
                     /translation="MTQEELFKKLIAHSKEYGFVFPSSEIYDGLSAVYDYGQNGVELK
                     NNIRRYWWEAMTRLNENIVGIDAAIFMHPTTWVASGHVGAFNDPLIDNKDSKKRYRAD
                     VLIEEWMEKQREKIDKEVEKARKRFGDAFNEELFRQTNPRVVELSESITTVNHRMVEA
                     LEQNNLAEVKKIIEDCEIVCPISGTRNWTEVRQFNLMFETKLGSVSDEANTIYLRPET
                     AQGIFVNYINVQKTGRMKIPFGIAQVGKAFRNEIVARQFIFRMREFEQMEMQYFVRPG
                     EEMKWYEFWMEKRMRWHRAMGFGDNKYRFHKHEKLAHYANAASDIEFEFPFGFKEVEG
                     IHSRTDFDLANHQKHSGKKLQYFDPDINQSYIPYVIETSIGLDRTFLLVLSAAYAEEK
                     VAKEDGTSDERVVLRIPPALAPVKLAVFPLVKKDGLPEMARKIMDDLKFDFACQYEEK
                     DTIGKRYRRHDAIGTPFCITVDHQTLDDETVTIRYRDTMEQERVPASRLREIMNDKVS
                     LTNLLRQI"
     gene            21600..24209
                     /locus_tag="PK049_02105"
     CDS             21600..24209
                     /locus_tag="PK049_02105"
                     /inference="COORDINATES: protein motif:HMM:NF025069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR/HmsC family protein"
                     /protein_id="HOK60378.1"
                     /translation="MVHLKPFITTITKYLVLLLAFTSAVHAQYLANFTNNKSTKIDEV
                     KPLSQIPEYKIKAALILNIASNIEWPNERNIKEFGILIVDSDTLIYNEIRSVETSYKL
                     KGKPIRVNLSSPPVSKPTEYSIIYLSHSYSPLVNSIYQQIVGKGVLIVTDEGTERLLT
                     MVNLFLNQEKQSVSFEINRHTLEENGFTLSPKLVALGGSFIDIKELYLKTYHQLIEEN
                     KKLQQLQTDLTRYQQEKTEYESEIRTLNERVNRLNQNTAVLEKQYANASVSLKQKDSL
                     LLIATKELAKKVAESQKLQIAINKQVALINSSRSQLDSLNKQIVQTQKVLDQKQKEIE
                     EQDIVISEKESIILKQQRRFFIMLIALVGISFTLVFAFWAYSIKRKLNIKLEKQVEIR
                     TNELNISRQHYQRLFENSPVAMLELDLSLLLNYVTSIGIPLDTMYEVDRSFSIAKVRE
                     GLKYIQVVNVNNAALKLLGFESKDDAIENYIRTYSEESLETFREVYKALIERRQFSEY
                     ESIRNTYDGKKLFVVLKWIVLPGYSHDYSRVLLSITDITKLKEYEKELTQHRDHLEEI
                     VQERTQQIIQLNKMLQESNEELLLKTEELEQTIHLLEDAQKQLVQQEKMASLGMLTAG
                     IAHEINNPINFISGSQQAIESLLDELWNSLNIYREKALKHSPKNITYELETNNSIQVE
                     ELYSSIKLMLRNIEIGIERTTGIIRSLKAFVRSDSKEYVPVSIPETIADVLNMLRGNY
                     KDRIEIIQHYDQDLPKVKCYPNQLHQVVMNLLANAIDAIPGEGKIYISGQYSSSDNNV
                     TIRIKDTGTGIPSDIQEKIFDPFFTTKDAGKGTGLGLYITYNIIKSLNGDIKVNSAPN
                     QGAEFTVTLTACE"
     gene            complement(24220..24594)
                     /locus_tag="PK049_02110"
     CDS             complement(24220..24594)
                     /locus_tag="PK049_02110"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOK60379.1"
                     /translation="MEKKVLYVDDEVINLELFKINFRNDFTILVADSAQKGLDILKNE
                     NINVIISDLKMPHMNGIDFIERIKHENPEKVCILLTAFMESEVMLRAINEELVYRYIM
                     KPWKKDELREIIESAFKRFENS"
     gene            complement(24670..26295)
                     /locus_tag="PK049_02115"
     CDS             complement(24670..26295)
                     /locus_tag="PK049_02115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrobacter succinogenes major paralogous
                     domain-containing protein"
                     /protein_id="HOK60380.1"
                     /translation="MLHRTTATGDVLYSETHTKTTTNQGLVAFEIGAGTVNAGTFSGI
                     SWSDPIFLELKVRKQDQQSFTDLGTSQIVAVPYAFLAEKSLNASNGISSLGPLGNTVF
                     SNGVEWAPTARVSVLDSTVIVSPGEGHDPDKPIFAVTNSQGQVVMAVYETGVRFNVEG
                     SSGKGAKGGFAVGGLTNGKAPVPTYFQLEPSYAQFLFDQTNIIKGAKGGFAVGGLTNG
                     KGDTLTYMTIHPDSTRFFIDDSPVVKGAKGGFAVGGLTNGKSGTNVYMSLLPDSIRFA
                     FDDSNTKGAKGGFAVGGLTNAKSSAANYLEVTPDSTYIVNTMVSYSDMVVAGNVTTNV
                     GLPEPPVADADGNTYQTVKIGTQVWMAENLKTTKYSDGTPIEPSNIYVLPTPDSTTVF
                     GNYYSDGAILLSGKNVCPDGWHVPTKPEWEAMFTFVSGAEWAIDSLINGKKLIEPLRD
                     VFPEIFNWYYDLHGPLSVVIATNETGFSARGAGRASWDTSWSATDVGEYAYFWVNTIP
                     GSVQIVTIDSYSGNVSIGEGSPGTAYSVRCVKN"
     gene            complement(26299..26445)
                     /locus_tag="PK049_02120"
     CDS             complement(26299..26445)
                     /locus_tag="PK049_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60381.1"
                     /translation="MKCLGKAMVVLLVLVGYVISAQVPQRIAYQAVLRNSDGTVMANQ
                     QVEL"
     gene            complement(26790..27914)
                     /locus_tag="PK049_02125"
     CDS             complement(26790..27914)
                     /locus_tag="PK049_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOK60382.1"
                     /translation="MNKGILELIVIMLIALPVGILTIRYFFKNSILFLMGALTVTWGV
                     LIDVMVNLKYIYPEIFPAYITTPVVIVAGIWVMWFIARKVRKPLDQAIDDLHRISDGD
                     LTVKVNPEFVQHNDELGRLSKSVGNLSDKLREVIDGISQAAGELESSASQLSMSATSL
                     SEVTSEQASSLEEISSAMEEILASIQQNSENAMQTERIASSTSKKLEEGVESTNVALD
                     SMNEIAQKIGIINDIAFQTNLLALNAAVEAARAGEHGKGFAVVAAEVRRLAERSRQAA
                     NEIIAVSQRGAEISGKAKDIMNQNVHEIIRTTDLIREITAATHEQRTGTEQVNTSVQE
                     LNNTTQQNASLSEEVAASAEELNARAKALTDLIGYFQIKK"
     gene            complement(27991..28812)
                     /locus_tag="PK049_02130"
     CDS             complement(27991..28812)
                     /locus_tag="PK049_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60383.1"
                     /translation="MIYIATVHWETDKWIDIQLKFFKRNINQPYRVFAFLSGDACKHK
                     DKFYFACCEPITEHAIKLNLLARFISLQANDDDILIFIDGDAFPVKPIDSFVTLTLKQ
                     YPLAAIRRTENIGDIQPHPSFCFTTVGFWKSIEGDWKEGYEWETTAGIHRTDVGGNLF
                     KILIDLKIDWLPLERTSQLTNHGLMFGIYADLVYHHGAGFREPLSMYDRTKSLVGIVK
                     PRFIAKLLDLLLCNVSVKNRFRIHRLLGIGDRINYQNQRLSQSIHEKIAMDEFFV"
     gene            28875..30047
                     /locus_tag="PK049_02135"
     CDS             28875..30047
                     /locus_tag="PK049_02135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOK60384.1"
                     /translation="MANPIQSPAVLIFTAETFPAMAGDGRNALLVASKLNENGYCVKL
                     ICLNPNGKLPKKEKLNGVEISRINYWYRTIIGRFALRLSLFRYLTINCNSSSKWIIFG
                     AMPGYKLIALVSTLKGVDCVFRSTLWGFDDAESVAGSLHRFYTRYLLSRLYGYYALNS
                     AFANSWRNVFGSFNILLSVQGVDLKKFDIDHKNGIRHEHRGKLQIAEGKFVILMVGHL
                     IERKGFPEIADWLSRIDEDFLLLHIGSTEAPEWDTMSRYNVGMKNLKKYVEEKLGDRV
                     RFMGRQNVTTQFYLLSDILLVASHAEGFPPNSVNEALAAGLPVLTLRMPGVADVITEG
                     VNGFVFTDESEFARKLSILINNPVRRNELGRNARVFALDKLDINKIIPTLQNFLGL"
     gene            31060..31764
                     /locus_tag="PK049_02140"
     CDS             31060..31764
                     /locus_tag="PK049_02140"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HOK60385.1"
                     /translation="MRLGILALLLIAATVTSTTISAQETRNFDAKGFDGIRNSTSAEV
                     QISQSPNYGIEVTAKSEVFNELEIVVKENVLHIKSKDNLRFTSNWGKVLVKVSCPDIK
                     LLAQNGSGNMIALTPIKGADEFTTSVNGSGDILLKEIQANSVQVSINGSGNIKTSAQG
                     KTYTLKANSNGSGNIDLETLTSEIAQLKLNGSGDATVRCNKEFKGSINGSGSITLYGN
                     PLIDAKVNGSGRLVRK"
     gene            31965..32384
                     /locus_tag="PK049_02145"
     CDS             31965..32384
                     /locus_tag="PK049_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60386.1"
                     /translation="MLRVAVDFDGTIVENEYPQLGKPMLFAFETLKELQKRGFVLILW
                     TVRTGKLLEEAVEFCRQNGVEFYAVNRNHPEEILDPETPRKLDVDVFIDDRNLGGFPG
                     WDKVFSMLCPNEPYEVSQKKVLKQYKTKSFVRRIFGI"
     gene            32395..33471
                     /locus_tag="PK049_02150"
     CDS             32395..33471
                     /locus_tag="PK049_02150"
                     /inference="COORDINATES: protein motif:HMM:NF016952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HOK60387.1"
                     /translation="MLQRISILLLVLAVACTNGRKDQLPDTTSMPAENPLHLIRFDKP
                     TSGSLFTAGESIDITIKLTDTIQPDSIVLYRNSERIQKVSNLSFTLQTQAGKPGTIQL
                     NATAWKDGRRQTASISIKLKSNTKPASYSYRIVKAYPHDPEAYTQGLFYHNGYLYEGT
                     GQQGASSLRKVELETGKILQSVNLSREYFGEGIAILDDKIYQLTWTNGTGFVYDAATF
                     KPLNTFSYTTQGWGLTTNGNELIMSDGSNTIYFREPSGFSELRRIEVFDNNGPVKMLN
                     ELEYIDGKIYANVYLTDRIVIINPETGVVEGNIDMQGLLTTTQRTGKEDVLNGIAYDP
                     NGKRIFVTGKLWGKLFQVEFIKKK"
     gene            33472..34914
                     /locus_tag="PK049_02155"
     CDS             33472..34914
                     /locus_tag="PK049_02155"
                     /inference="COORDINATES: protein motif:HMM:NF012432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HOK60388.1"
                     /translation="MSTRTETDYLGSLQIPADALYGIHSVRAKDNFPDTTQFHVEWYQ
                     AMGLVKQACYLTYAEMVKALQEENLFRDSKGFIPPNVVNALIAVAGEVHEGKYFDSFI
                     VPAIQGGAGTSINMNVNEIIANATLIRLGHNPGNYTVIDPIEQANVYQSTNDTVPTAL
                     RVAVVRLLTLLEELINNTRQAVETIEKSYRNSLRIGYTQLQAAVPSSYGQLFSTYSEA
                     LSRDWWRVSKCFERIKVVNLGGGAIGTAVGTPRYFVMEVVKHLQQLTGFPFTRSENLN
                     DATANLDSFVEVHAILKAHAVNIEKMASDIRLLASDIAGNAINIPKRQVGSSIMPGKV
                     NPVIPEFVVSIAHRVYTNDMLISNLAGQGALDLNAYLPTIGHAMIDSIKLLIAANQAL
                     AGHLLNGIEIDSEKSAQLLMFNPSITTALLPIIGFNRAAMLAQEMRLKKIDIFSANQN
                     LKLIPEEKLVELVKPNNLLKLGFSFREVID"
     gene            34996..37269
                     /locus_tag="PK049_02160"
     CDS             34996..37269
                     /locus_tag="PK049_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60389.1"
                     /translation="MKKFTEYIVVVAVGLLFTYLFAGDVVKNINHRYFAQSGDGAKDY
                     YATFYHIKHDKSYIHSNSMNYPFGESIFFAGSQPIVSNTVKFISQNIYDVSPYTVAIH
                     NLLILISIALTVLVIYLIFKHLNLPIWYSLLVAIGITFLSPQIDRIGGHFALSYTIVL
                     PLFILLLLKFDRQKSIGLSVLILFAGMWASITHGYFFTFYMVMFLAYWLWQKPWRKEF
                     EWRKQWLHVVLQIAIPLIVVPTLILYIEPVEDRTTWPWGFLFYRAFPESVFLSPGRPY
                     WGWLNSLVKIRNLQWEGIAYVGLVSTIIFLIGLFSFIKNAVNRRWGKTLTITSQPLMD
                     RLFWLSFLLLLFSFGIPFIFKMEFLLEYLGPIRQFRGIGRFAWLFYYTMNIVAFYLIW
                     QKAKGYKPVYRILLVIPITILLYDAWFHSKSQNHFINNSLPELDYAFHSNSKPNINID
                     EFQAIMPIPFFSVGSENYWHESACFGSMVKMYALSQLTGLPLNATYTSRSSVWQCVEN
                     LNLIMEPLQEYPVLKYYNHGKDILLFVDPHCDQINQNERRIITISTPIDTLWGYELRR
                     LKVADLAKLPEVYRNEYLNATPGMADTISGNYYFQEFESTPTPLAFTGNGALKINRRG
                     YTNLYWGRLPNNSSSEITVSFWVNRMKADIVPRNRIEIVTGKEEGKWETWNGFGFMEK
                     TKAFWNDWALVEYNLKLNNPSDFICISVAPLLAKSGDVYIDNVLIKPSNFNVKFVMGD
                     FVLLNNRLFNNISIGSD"
     gene            complement(37266..39314)
                     /locus_tag="PK049_02165"
     CDS             complement(37266..39314)
                     /locus_tag="PK049_02165"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOK60390.1"
                     /translation="MKAKHLLLSLFVFVALVSNAQLVTDPQQVVADILEEMAANSDTE
                     QDYSELVEDLLQLAESPLNLNAARKSDLQKLFFLTDFQIESLLSYRDSTGKILSVYEL
                     QLVPGFDLTDVERLAPFVVAGEVVKPLKVSDFTMGRHDIFTRLKTTLQTPVGYRSDYG
                     GASSYLGSKYAIYTRYRYRANRLQIGVTAENDAGEPLLDGTFPSGFDYLAGYAMVSDV
                     GKVKRLVVGDFRAEFGQGLTFWNSLTFGKSANVMGLHKRGRGIVPHSSAYESLFLRGA
                     GFTLNFKHIDFSLLASYQKIDGNLVDTVSSGELAFSSLPETGYHRTASEIASRRNIPE
                     LVTGSNITLTFNKLRLGTTAVYSRIYGENLNNQPIYSLTPASTEKLAMGINADYYYRQ
                     HLFFGEIAIDIFSQKMAALAGGQLKLSNRVHLSVLGRSYSTTFNPRYTAALAEGSGAT
                     NEKGLLMGLSLLAAKGWKISGYIDLYQFPWMRYGVYSPSAGRDFLFLSEHSLNTYFQL
                     SFRYRYKQDEKNMTGANIPVTSTLTQTRQGFRTQLTYQPNPLVNLRSTLEISNYRTDS
                     LSGGEYGYLLAQDININLQKTPLSIRLRFAIFDTESWNTRLYSYESDLLYSFTVPAYY
                     SNGTRIMAMLKYSPSKGVDLWLRYAQTYFPGSATIGSGPDLIMGNTRSEVKCMVRVKF
                     "
     gene            complement(39311..40153)
                     /locus_tag="PK049_02170"
     CDS             complement(39311..40153)
                     /locus_tag="PK049_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60391.1"
                     /translation="MELFRQLSIVLLLLFQALSVYSQADIRPISGAAAAMGNCSLSMQ
                     GNWDGFSNQASLAYQRQFWVGAHHENRFMLSELGLSMLGVAIPVKPGTFGVDISHLGF
                     SGFSTSRAGLGYGMFLGKRFAAGVGLALTRMQLPADYRNATAFTVEGGVHYMPTSKLT
                     FGFYVFNPTGSTFDDLGDLPTLMGLGVTYLPSQNILLAFQIDDDTQRAPTFRFGLEYR
                     VMERTALRLGYSAASPEGITAGVGLPVKGFDIDFSLSHHQQLGFTPSLSVAYLFGKKD
                     NKTQ"
     gene            40307..40846
                     /gene="hpt"
                     /locus_tag="PK049_02175"
     CDS             40307..40846
                     /gene="hpt"
                     /locus_tag="PK049_02175"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005657966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOK60392.1"
                     /translation="MKQVKLKDKEFRLSYPEAEIQNDIDLVASKINHDFAKSGEVPFF
                     VSVLNGSFMFTADLLKRITVPCEVSFVKLASYQGTSSTGKVNELIGLNENVTGRTLVV
                     LEDIVDTGITLSKLYEELRKLNPKEIRVATLLFKPEAYKGNIKLDYIGRSIPNDFIVG
                     YGLDYDGLGRNLPDIYTLI"
     gene            40996..42036
                     /locus_tag="PK049_02180"
     CDS             40996..42036
                     /locus_tag="PK049_02180"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOK60393.1"
                     /translation="MDSLVNSHFIIPFLISMFLAINMGGSGTGPAFSVAFGANVLKKT
                     SVPALFGLMVFLGAIIAGKATSATVGKGLVNPELMNYAQVSIILFAVSISLLVANLFG
                     IPQSTSQSTVLAVIAPGIYFHELNSRKVFFEVIPTWFILPIIAFILSLIIGKYIYTPL
                     RRRGYTISSRINDHYMLRWAIIFASLYVAFSIGANNVANASGPLTSMTMNELGLGNQG
                     GRLLILIMIMSTMIVAPSFGIGSSLFGEKILRNTGKELFLFGRIEAVIIAIISATLLL
                     AASLFKGIPTSLVQLNVGAILGIGVARLGPRNIFRKTAVRKFFLMWLIAPIIAFLISW
                     YLTFLADKYGYL"
     gene            complement(42132..43289)
                     /gene="dnaJ"
                     /locus_tag="PK049_02185"
     CDS             complement(42132..43289)
                     /gene="dnaJ"
                     /locus_tag="PK049_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOK60394.1"
                     /translation="MTTKRDYYEILGVPRNASKDEIKKAYRKMALKYHPDKNPGDKDA
                     EEKFKEAAEAYEVLSDDNKRARYDQFGHAGMSNGAGGGGFSHDWTIEDIFAQFGDIFG
                     GHFGSFGGFGGFGGGRSSRRTNRGTDLRVKVKLSLSDIANGVEKKIKVNKYVSCQSCG
                     GTGAAGGSSYSTCSTCRGSGYVTRVTNTLLGQMQTTSPCPTCNGEGKVITNKCVACNG
                     EGVVREDEVITVKIPAGVAEGMQLNVQGKGNAARRGGVPGDLLVMIEEEKHPNLVRDG
                     NDLIYNLNISIPDAVLGAPAEIPTVDGKVKIKIEPGTQPGKILRLRGKGLPEVNSYQR
                     GDLLVAVNVYIPKNLTREEKAIFEKFAKSPSFTPNPDEGGSFFERMRGMFE"
     gene            complement(43302..43895)
                     /gene="grpE"
                     /locus_tag="PK049_02190"
     CDS             complement(43302..43895)
                     /gene="grpE"
                     /locus_tag="PK049_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004297892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOK60395.1"
                     /translation="MSKKHKKEDIEQEQVNQQTTSTESNVTDSNTQAPQGDAQQTEAS
                     QANDDKVAELTTQLEEMKDKYLRLTAEFDNYRKRTLKEKAELIKFASEEVLKDLLPVI
                     DDLDRALKAIETANDINAVKEGISLIVNKFNDFLKAKGVKEIDAIGKELDTDLHEAIT
                     KIPVQDDAQKGKIVDVIQKGYMLHEKVMRFSKVVVGE"
     gene            complement(44124..45842)
                     /gene="recJ"
                     /locus_tag="PK049_02195"
     CDS             complement(44124..45842)
                     /gene="recJ"
                     /locus_tag="PK049_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOK60396.1"
                     /translation="MEKRWVLKDPGDPVLVSSLAKQINVNETIASLLVQRGVDTYEKA
                     KRFFRPSLADLYDPFLMKDMAEAVERIEQAIRRGESILFYGDYDVDGTTAVALMYSFF
                     RKRYSKIGYYIPDRYNEGYGISFKGIDFAFENGYSLIIALDCGIKAVEKVKYAKLRNI
                     DFIICDHHLPGERIPEAVAVLDPKRSDCMYPFKELSGCGVGFKLLQGYCKKEGISEEE
                     LYQHLDLLAVSIASDIVPIVDENRVLAFYGLEKLNTNPSRGLKSIIKIAGIENQRIAI
                     DDIVFRIGPRINAAGRMESGKTAVDLLCASDDSSAFEVGALIDSCNNDRKNVDRTITH
                     EALRMIAQDKRLQHSYSTVLFNPSWHKGVVGIVASRLIETYYRPTIILTQSGDLATGS
                     ARSVQGYDLYQAIEACSDLLENFGGHMYAAGLTMKVDRVEEFKRRFEEHVSKTITPEM
                     LSPQIDIDTRIRLDDITERFFNVLKQFQPFGPGNMSPVFMAENVYDNGEARLVGSEKE
                     HLKLTLVQEGSTQKFDAIAFQMAEYYKPIHYGQPFDACFAIHENNYKGNITLQLRVKD
                     IKLRAK"
     gene            complement(45861..46553)
                     /gene="lipB"
                     /locus_tag="PK049_02200"
     CDS             complement(45861..46553)
                     /gene="lipB"
                     /locus_tag="PK049_02200"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HOK60397.1"
                     /translation="MQNVKFHDLGTVDYKEAWDYQEKLFDEVTKRKLNGDGKLHYLLF
                     CEHPHVYTLGKSGENANLLIPEEMLKRINASFYHINRGGDITYHGPGQIVAYPIFNLE
                     AFGLTLKSYIHLLEQVVIDCLQEYGIVADRLEGATGVWLDPGVKGRERKICAIGVRAS
                     RYVTMHGLAFNVNTNLDYFRYINPCGFIDKGVTSMQVELNRQLDMEEVKAKLLNHFAH
                     RFHFACNRVGTV"
     gene            complement(46603..47469)
                     /gene="lipA"
                     /locus_tag="PK049_02205"
     CDS             complement(46603..47469)
                     /gene="lipA"
                     /locus_tag="PK049_02205"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOK60398.1"
                     /translation="MGNERKPEWLKIKLPGGEGYAQVNSIVKKHGLHTICSSGMCPNI
                     GECWGNGVATLMILGDICTRSCKFCATATGKPLPPDPNEPTKVARTVSLMGLSYCVIT
                     SVDRDDLPDHGAGHWRSTVMEIRRVNPNTTIEVLIPDFDGRPELIDLFLESEPDVVGH
                     NLETVERLTPLVRTKASYRRSLKVLEHIAKRGFRAKSGIMLGLGETRDELIQTLDDLL
                     AVGCKMVTIGQYLQPRPTNLPVQRYITPEEFEEYKQIALAKGFEFAESGPLVRSSYHA
                     DKAQHLVKGCCK"
     gene            complement(47812..48447)
                     /locus_tag="PK049_02210"
     CDS             complement(47812..48447)
                     /locus_tag="PK049_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005865390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HOK60399.1"
                     /translation="MSRYIAGLISQGEGLTLDFKHSITDSRKIARSLVAFANTAGGTL
                     LIGVKDNGNITGVNTDEEYYMVEAASQLYCKPEVPFEVVKWEINGKTVLEVKVKPSRR
                     KPHKAPDKTGVYKAYIRVNDENVVASPVQIKIWKAEKYRKPINILFGEPENALIDYLK
                     SHQTITIDHFSRLGFISVSEAEKILINLTAMGLIEYSNLNNEDHFSLRMAE"
     gene            complement(48489..49046)
                     /locus_tag="PK049_02215"
     CDS             complement(48489..49046)
                     /locus_tag="PK049_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HOK60400.1"
                     /translation="MQKVIVAVTGASGAVYAARVIEKLIELSARVDEVAVVFSENGKR
                     VWEYELNRDITFGFPVKLYDNNDMFAPMASGSAGYNAMVVVPCTMGTLGRIASGTSND
                     LIVRAADVMLKERRKLVLVPRELPYNLIHLQNMVTVTQAGAVVLPASPSFYSRPSNMD
                     ELIDTVSDRIVEMLGFAIEHYRWGH"
     gene            49341..51623
                     /gene="rnr"
                     /locus_tag="PK049_02220"
     CDS             49341..51623
                     /gene="rnr"
                     /locus_tag="PK049_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HOK60401.1"
                     /translation="MTKKKNKTDAGSKPNRIAKKELKKLVTDLLLANPTNIYNYKQVS
                     NKIGVSDDYTRRMVNEILYELALDGFAEELQRGKFRAVMQNALAVGILEMNPDGSADV
                     ITDDNREIFVPDFRLNHALHGDKVQISIYRQRRRGLLEGEVVRVIERAKRNFVGTINF
                     VGKNAFVVPDNKLMPYDIFIPKVELKDVRNGQKVIVQITDWPAREKNPNGKIVEVLGD
                     PGVHEVEMHAILAEYELPYRFPEELDILAEKISDKITEADYRERRDFRNVPTFTIDPF
                     DAKDFDDALSVQTLPNGNFEVGVHIADVTHYVKPNTPIDNEAIERGTSVYLVDRCVPM
                     LPERLSNYICSLRPNEEKLCFSAVFELNENAEVLNQWFGRTVILSKRRFNYEEAQQVI
                     ETGKGDMSEQILLLHKLAQKLRAERFKKGAIAFERVEVKFDLDTKGKPLGVYFKENKE
                     SNQLIEEFMLLANRRVAEFIGMRKDGRNELPFVYRIHDKPNEEKLNAFRSFITRFGYS
                     ISGTTDKQVSKSLNQLMEKVKGRPEQNLIETLALRSMAKARYSTDNIGHYGLAFRYYT
                     HFTSPIRRYPDMMVHRLLAHYLNNGKPKDREQIEMLCKHSTNMEIKATEAERASIKYK
                     QVEFMQDKLGETFTGVISGVTNFGIFVELTDSQCEGLVSIRDLDDDYYRFDEEEYALV
                     GQRTGRKFTLGDEVEVEVWRTNLAKKQLDFKLVGMKGRSSKPLKPRRSQTKSSPKNPK
                     VASSRKGQQKAKKKEKRRKR"
     gene            51637..52863
                     /locus_tag="PK049_02225"
     CDS             51637..52863
                     /locus_tag="PK049_02225"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOK60402.1"
                     /translation="MKRFIVIALAWAAFACSTKNELTPGSWLGVIQMDSIPGRLDVPF
                     NFEVKVDNDKVQLTVRNADELIEITEVERIGDSLYAKFPTFTSELVMAVNDSLGGYYY
                     PKGVKDGRRYKFYGIKGETDRFPWFTEQPKFDITGRWWFIENPGTPDSTVMVAELKQE
                     GSRVTGTILSTTGDYRYLEGKVAGNMFYFSKCDGAQSIIVRGKITDSATMVNGLISGS
                     PRWASAWRALRDDKAQLPEAQSLVWVRKGYTTFEFAGTDLNGSRVTSNDERFKGKVLA
                     VLAGGSWCPNCLDEGRFYKTMYEKYRDKGFEVVSLCFEDKTFEAAQPKIKRFAQSIGA
                     SYTFLYVAPRGREQRDSVLYALEGQMAYPTSMILDRNGKIRRVETGFSGPGTGEHYQK
                     FAHETEELIVKLLEEK"
     gene            52926..53561
                     /locus_tag="PK049_02230"
     CDS             52926..53561
                     /locus_tag="PK049_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008774483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HOK60403.1"
                     /translation="MNDLLAFGLLGFTSFFTLINPLGAMPIFMTMTAELNPAERIKTA
                     RKASIVAFLTILFFAFTGQLLFRFFGISVNSLRVVGGVIFFMMGMDMLQARLTRVKIA
                     ENEVKKYVNDISITPLAIPMICGPGAITNAIILMQDATTIDKKMVLVFSIFLVMLLTF
                     LILYSSSKIIKALGETGNNVLMRLMGLIVMVIAVEFFFAGIKPVLIEIFGK"
BASE COUNT        15820 a        11821 c        11231 g        14764 t
ORIGIN      
        1 ttgaataacc gttcgtcttt tttaacctca caagactcaa gtaccgatgc aatttttcga
       61 agagttgaat gatcaacctc cttgaaaaga agaattgacc ttaaacagtc aatccttttt
      121 tcgagctcat catccctatg ttttttaaat aaatttagca tggaaatttt ttgaataaaa
      181 tggtactgcc aaattaaata aaattcctta atatctgcat aaacaatcat ttttttgtaa
      241 tattgtgaaa cgtaaatggc ataattaaaa cggctatgag attaaaactt accatccggc
      301 agaaaatact atactacatc ctgggtagtg catttattat tttttcaata gttttttact
      361 tcataagttc atcgtcgcgc cgtttagcat acgagcaatc cattaagcta accaatagtt
      421 atgcccgaca gtacgccctt aacattgaaa gttggataaa ccaagatttt gctgtaaccc
      481 ggacgcttgc tgcagcattc atggagtaca agcagatgcc ctttgatcaa tggcaaaaac
      541 taatctaccc catgtacaat agggttattc taaccactcc acatatagat gcttactggg
      601 atagctggga gttaagcaat cttgacccca actggacact cccttatggc cgatacttct
      661 acattgttta taagcaagac ggagtttaca aaacaaaagc tgaaatgcgc agcttaaccg
      721 gcgacccccc aacctacgat agcatgaaaa gtgctgccat ggagattgtt gttgaaccat
      781 acatatcgga gctacaacag ggacaaatga tgacaaccct ctcatcgcca cttctcgaaa
      841 atggtaagtt tattggttta attggtgctg acctaatact tacccgcttt cagcaactgg
      901 taaataacat taaaccttac cccaacagtt acgccttttt actatccaat aagggtgttt
      961 ttatagcaca ccccgattcc gcaattttca aaaagaacat caccgaagtt ttaccagagt
     1021 tcaataatca acacaacata cttgcccgtg ttcagaaagg gaaagccttt agtttcatcc
     1081 atcaagatag caagggcgag aggatatact acacctttga accaattaga ataggcaata
     1141 cgcaaacacc atggtcaatt ggtattgctg ctccggttac tgatatcatg gctcaaacaa
     1201 acaggagcta caacacaagc cttctcatcg ccctgatagg tttactggta attattttta
     1261 tgggttactt cgtttccaac aacattacat tgcccattaa aaaaatcacc caaattctaa
     1321 atgagtttgc aaagggaaaa attgtaagca gcattgacat tagtacctca tcgaacgatg
     1381 aaataagcca aatggttgag gcggtaagtg cctcgcttgc aggagtaaac tctaaaacca
     1441 catttgcccg cgagattggt gcaggaaatc tgaatgtgaa cctttcgttg ctaagcgatg
     1501 aagatcagct aggcaagtcg ctaattgaaa tgcgtgataa ccttcgtaga gcacgtgatg
     1561 aggaagaaaa gcgaaagatc gaagaggaaa agaagcggtg gactaatgag ggaatggcaa
     1621 agtttggcga gatacttcga caaaacaaca acaaccttag cctgctaggc caggaactaa
     1681 ttaaaaacct tgtttggtac cttaacgctc aggttggtgg gttattcgtt ttgagcgaaa
     1741 acaaggaaac tggcgataaa accttcgata tggttgccat gtttgcctac gacaggaaca
     1801 gggttatgca acgaagctac cattttgccg aaggtcttat tggtgcttgc gctgctgaac
     1861 gcgatgctat cattttgaaa gaagttccac aggattatat agagattacc tcagggcttg
     1921 gtggtattaa ccccaattac ataatagttg ttcccttgat atttgaagag gaggttatgg
     1981 gggttattga aattgcctcg cttcaggagc ttaaagaaca cgaggttcag ttcctcaagg
     2041 atattgccaa aagcattgca tcgaccctgc ataccgtaaa ggttaatacc ctgactgctg
     2101 aacttttaat gaaatccaaa gagcaagcgg aaatgatggc tgcccaggag gaagagatgc
     2161 gtcagaacat ggaggaacta caagccaccc aggaagaggc tgcccgtaaa acccttgaac
     2221 tcgaagggtt agttaatgcc ctgaatgctg ctgcttttgt tatggagtac gataccaagg
     2281 gttacgtgat taacatcaat gatagttacc tcaacttcct tggagccaat cgtgacgaca
     2341 taattgggtt acaccattct gaaaatataa ttttatccga tactgaaaaa gcctcgtacg
     2401 ataagatgtg gagcgatatt ctcaggggaa atatccgtaa gcataaaacc cgcattaggg
     2461 ttaatggaca ggaacatcta cttcttgaga cctacactcc aatttttgac catggcggtc
     2521 aactgttgaa aattctaaaa attgcaacgg atataaccaa catttaaaag gtaatatcaa
     2581 ccaaatatcc tgaatgattc cgaatattga ttacaatacc tgattcaaaa atcaggtatt
     2641 gtccttttat accaaccagt ttaccctcaa ttgaattaac tttttccagg ttaacggatc
     2701 ttaccttatc tgaaagttga attactggat agcttacacg gtaaaccaca tcgctttcag
     2761 gaatgtaact taaattttta ccatttataa attcaagcgc ttttgcttta tgggtcaaaa
     2821 aatcgggcgg tagctcgctt gaatcggtga gcatacgttg ccaagccgtt ttatcggcaa
     2881 aaatcttttt tagctctacc tcagccaatc ctgcagtata acggttagga gtatgacaga
     2941 tctttacggc aaaggttgct ccctgatcaa cccatcgggt gggtatttgg tgataccttg
     3001 taacaccaac ctttaagcca ccagtccatg ccaggtaaac gtagtgttgc acaagacaat
     3061 ttgcctttgc aaattccaaa tcgcgggcaa taccctcgtg tgctctgcac agttcaggcc
     3121 ttaaaacgca ctcctccgct tccggtacct ttataaaaca tgggtaacaa aacccctgac
     3181 caaacagctt ctttgtttca cgcccacaat gaatacagcg attaatattt aggtacttca
     3241 gccctattcc ttttccaatt agttctgaaa cgtttaccct acctgaatca agatcaagaa
     3301 aatactctgg actaatacta acatcattgc cagtattagt gaaccccatt tttgtaagca
     3361 cacctttcat ggcagattta ttttaatgag taagttgaat tttatatatc ctcgcccgct
     3421 gaaattgcta aaacacagaa taaagaacat agctcaacca atgaattata aagataaaca
     3481 tttaaaaaga aagcttaagg ttctacaaaa tgactgcaat gttaaaccat gttaaatata
     3541 ctactatgta ttgcatagta ttattttatc gcttatattt gtaataaata aatactagtt
     3601 attgcattgc aacaaataaa aagtcaaacc aaacagcatg agctatgaaa cgtacaattt
     3661 cagtgagttt aaacggtaaa gcctatatgc ttgaagatga tgcctaccat gccctagatc
     3721 gatatcttaa gggtctggag gctcactttg caaacgaccc caacaaaaag gaaatagttg
     3781 gcgatattga aagtaggatt gccgaacact tcgatgaatt cataaaggtt ccagggcaag
     3841 tggtaaacct tgaagaggtt aatcgggtag ttcacctaat gggaaacatt aacgactttg
     3901 accccacaaa cactaaagcg tcttccgaaa atgacagggt gtacccaaaa ctttaccgcg
     3961 atgtggaaga cagaatttta ggggggatat gcacgggtat gggacactac tggaaagttg
     4021 atcccgttct tttccgtttg gtatttttcc tgctaaccct ttggggaggt ctaggcgtac
     4081 taatctacat tgttttatgg gtagttgttc cacccgccat taccccaaca caaaaacttg
     4141 agatgaaggg tgaaaaaata aatgttaaca atatgtaacc catttttcat agcaaagaat
     4201 gggttgaatc agaaaatatg cacaacataa cagaaaccct aagttgaagc catgaatcag
     4261 gaaatagaga atgctaaagc acaaatgcga aagggcgtac ttgagctatg tgttttaaca
     4321 gtactatcga gaggcgatgc ttacgcaaat gaccttataa accagcttaa acaggctaaa
     4381 atgatagtag ttgaaggtac tatttacccc ctacttactc ggcaaaagaa tgctggttta
     4441 ctcacctatc gatgggaaga gtcgccgcaa ggccctccaa ggaagtacta ttcgctaacc
     4501 gaagaaggca aagagtatct tgctgaactg ctaaatagct gggatgaact ggttgaaatg
     4561 gttagcgcta ttcgcaatca gaaaaatgat taatttttag gtactattac catttttacc
     4621 attaataaaa aacaaatgaa tacaactgta aatgtaagta ttggaggcta ctcttttaca
     4681 ctggaagaaa atgcatacaa tacgctccag gagtacctta acacggttaa ggtaaaactt
     4741 ggcaacgata aggtagccca ggaaactgtg aacgatattg aattccgaat agcagaattg
     4801 cttaccgaaa aaattacctt aggtcaagtg gttaccaatg aaatggtaac gccaataatt
     4861 gagcaaatag gtaaacctga agatattggt gaaaataacg actcaaccag caataaacag
     4921 taccgtccaa aacgtttata tcgtaacccc gacgattcaa ttattgctgg cgtttgcggt
     4981 ggactggcag cctacttcag tgtcgacccc ctagttttta ggattttatt ttttgctctt
     5041 ttattcctaa aaggatttgg cttgctactt tacattctac tatggattgc aatgccaaaa
     5101 gcaaagaccc cattccaaaa aatggaaatg atgggcgata ctgagaacat tgatgaaatt
     5161 agcaggcgtg ttcgaaaaga gattagcaat gcaagtgata atctaaaaaa atcaaaggca
     5221 ggtaacactg ttaataaatt aataaacctt ttcggacagg ttatgctcta cctgttaaaa
     5281 gccatgcttg ttattgttaa ggtaatttct attaccatag gagttctttt aatcgttagc
     5341 atgctactta tttttatagc ccttgttggc gctgtgtttt ttgcatcgta tagtcccgat
     5401 ttttccggag ttattggtgg cgttggaatg acactcaatg aaattattgc atcggttctt
     5461 gatattagta gtagctactg gattacaatc ccactatttt taactctggc cataccaatt
     5521 gttgcactaa tttatgctgg aatacgcatt cttttcaggt tcagggctaa agatggcaca
     5581 ataggcatta ttgctactgt aatttgggcc gctgctgttg taaccctttc actcacattt
     5641 ttcttccaga tgcgaggcct ttccgttagc gaaaaggtgg taaaggaatt ggaaatttcg
     5701 ccagtaaata gcaactctaa gattgtattg tgtaaatcat acaactacat gaccgatagt
     5761 acatcatcga tagaggaaaa ggttcaaatt ttcgattgga aaatagttac actaaaaggg
     5821 aaacatcagc ttgcaggctc acccacgctt attatagaca agggctttga agcgaaacca
     5881 agatttgagg taatacgcaa agcaagaggg gttaacagct atacagcagc agataatgca
     5941 aaggacatta tttactcctt aacattaaac gataccctgg ccattctgga tccgttatac
     6001 attatccctg aaaaaacaaa gtggaaaaac cagagcgtac tcattaagct gtatttgcct
     6061 gaagggtacg gtgtttacct ggacgaaacg ctaaccgaaa tgcttgaaaa atatcaaccc
     6121 tacagtaact actggcccga tgaaatggtt gggaaaacat ggattatgac cgcaaacggt
     6181 ttgcgtattg cgaggtaaat aaatttttaa aaaatatttt gaggtttact acattagtat
     6241 taattttgca ctgctttaaa aaagcgatag gattgaccta tggtgtaatg gtagcacagc
     6301 agattttggt tctgttagtc taggttcgag tcctggtagg tcaaccaaag cccctgaata
     6361 aggggctttt ttattttaga accggaaata gataaacggc ttaagctcca tggtttgaaa
     6421 ttgaatgaga acaactacta ccaacagagc tgcaaaaagt tttaaactca aaggcatagc
     6481 aataaaccga ccccgcagat actccttggc attatagggc aataagtgga caataaagcc
     6541 caacgcaaga accaggaata catcggaaaa agccagagca atatccggga tagttttagt
     6601 cccgaaactg gtaaatattt gacttagcat atcggttgcc gcattaaaat cgtttgccct
     6661 gaaaaaaatc cacgacaagc aaaccacatt aaaggtgata atgccccaga tcaatcgggt
     6721 tgaatgtctc tcgctattaa tgtttactaa tcttaagttg gatagcaact tttcaattgc
     6781 caatccaacc ccatgaattc caccccaaac aacaaatttc agatgtgcgc catgccataa
     6841 accaccaagt agcatggtta ttaacaggtt tatgtatgtt cttatccttc cttttcggtt
     6901 tcctcctaag ggaatgtaaa ggtaatccct caaccacaac gatagcgata tatgccaacg
     6961 gtgccagaaa tcggttagac tggttgcttt gtagggagag ttaaaattga ttggaagctt
     7021 aaagccaagc aaaagggcta ctcctatcgc aatatcggtg tagcctgaaa aatcgcaata
     7081 aatttgcatg gcatagccat aggtagccag caggttttca aaaccagtat agccgagggg
     7141 attactaaaa accctatcga caaagtttac cgacaggtaa tcggaaatta ccattttttt
     7201 caccaaccca gcaagtacta gaaaaagtgc atggccaaac tcatttttgc ttaattcata
     7261 ccgccggtaa atttgcggaa taaactctgc agcacgcaca attgggcctg caaccagctg
     7321 agggaaaaac gaaacgtaaa acgcaaaatc aataatgttt ctgaccgggg cgactttccc
     7381 ccggtaaaca tcaaccgtat agcttatggt ttgaaaggta tagaatgaaa ttccaacagg
     7441 aagcacaatg gtggtagtgt caaatttcgt attaaggagg gcgttgcccc aaagggcaag
     7501 atgattaaca actgtaaggt tgatgcctaa aaggttattg agcaaatcaa caataaagta
     7561 tgcgtactta aagtacgaca atactaatag gtttaccaaa acacttaacg ctacaagcag
     7621 ctttctattt atcttgctac ccgatttagc tattgctaat ccaattgtgt aatcgaccaa
     7681 ggttgaaaac agaagaagtg agaaaaataa cccgcttgat ttaaagtaga agaaaaggct
     7741 aaataccaaa aggtaagcac ttcgtataac cggacggttg tatacaaacg tgtaaccagc
     7801 cagcaaaacg ccaaagaaaa cccaaaagga aagctgggtg aatatcaggg agtcaccctc
     7861 ggtgtagtga agaaagttca gaactatttc cttaatatta atcataacaa ttggctactc
     7921 aataaaacct gaattataca gcgcatggta aaacaactcg ccttggagct gataaccttt
     7981 tgctgtaaga tgaaccccat cgggggctgc aaaaccatac ctaatccatc cctgaatact
     8041 tcccttgcca cccataaggc tatagaaatc ccacaccggt agttgatgtt tggttgaaat
     8101 ctctttaata acattggacg ccacctgcac cctgatgttc atgtacttac gatttaatag
     8161 atgatcgttt ggcgttgtaa gtattataaa tgggttaggt aaagcgcttt ttattgtgtt
     8221 aattaaattt tcctgatcac gtgcaaaatt gagactatcg aataagtttg aatatgcatc
     8281 attggtacca agtgaaacta ttactatatc gggtttaatc aatttgatat tggggagcaa
     8341 ttgttttgcc aatgaaaatc cgtggacagt tgcaccattt aagcctacag aactaagaga
     8401 aaatttcgat tttgtgttcc aaagattcac accataaact gtacactcaa aatcattttt
     8461 tgccctaaac cctattacaa cactatcaac ttcgctctca agttcaaact caacaaaatt
     8521 ttcatgtaaa ataggattta catttttggg ttcaaccaga ataggagtca agtaggatga
     8581 agctgattgg tagaatacac ctaccctgtt tacctgtgtt ttgaagaggg cgttaggctt
     8641 tactttaaac gatagggttg caaggctatc ggtggtttta gctgcaaccc caaaaatacc
     8701 tgcatcaacc ggagctttgg ctccgtttac cttttggtac tcccatcggc cagtatggta
     8761 tgaggtgtac tcaacaggat tatttgaacc cattactgag taagggaaaa ccaaaccagg
     8821 cgaaacatac tggctatgga actcgcgtgc aagaaacatt tttactgttc tcgataatat
     8881 tccagcctgc acatgtgaat cgccaatatg aacaattgtt agcggcttac tgggtttaac
     8941 tactattttt tggccaaaga aattttttat tgtgttagcg gaatggttga ataataaaat
     9001 ggcaggtgat tttgtgggta ccagtaggct tacctgatat ggcaagagtg tcaagattaa
     9061 aaaaatggca atgccggtaa tatgaagtac taacctattc atggcttact gaatcaggga
     9121 aagcagtggt ctggttgtta ttacctaagg ccattagcaa tgcattagcc attttttggc
     9181 caaagcgctt agccccataa tttgtaaagt gggtataatc ctttgttgca aggggaggtt
     9241 tagagttaac ccattcaatt attgaacctt ctccacccat agcggaatac ccatcccaaa
     9301 acaaagcccc attacctaaa gccgcatatc tttgagcctg cagcactttt cccacgcttg
     9361 ggttcgatct cagctcgcca tcgaaccggt tggccatatc ggtaacccca acaactaaaa
     9421 tatcggcttg tggagcaaaa cacttaactg cttttatttg ttgctccata agcttttggt
     9481 agtaggcata gcttgatagg ttcgagggaa ccacattgat tccaaactgc attataacca
     9541 atcggacatt tagtattctg taaatttccg aggcaacctc tgaatcgaaa tggttaaaaa
     9601 agttaccgga tgttcccctt aatgcaatat tgcaaacact aacaccgctg cccgattcgg
     9661 ccagtaagcc ataaagctta acggtagatg gattccttaa ttgaattgag attctctcaa
     9721 aacggctggg aaggtttact tttaccaatt tcactttgct gtaaggcaca acctgcgtct
     9781 ctagggtatc ggttccatca actataattt taaatgcata ggaagaatca gagttacctg
     9841 aaattatgct taaggtgtta aagctttgct tagagttaac ctttatggaa acaatctcgc
     9901 gatacaaatt cgatggggca acctcagcaa ttgacccaag cacacaatcg ggcttttgtg
     9961 ttggtttggc cttaaccgtt gaatttacat ttacatgctc tgataagcta accctaaagc
    10021 cgtatggcaa atactcagta ttccaaattg gaattagacc caaacccttc ccaccgaact
    10081 tttcctgaag ggcagctctc agctggctgg tgatcctatc gccctcaatt tgcgaatcgc
    10141 cataatgcag cactcgaacc gttgtaactt catcgctatt cctatatata gcgctaataa
    10201 aggagcctaa cgcattaata ccctgtggca tttcaaggtg taaggttccg gctgtaacaa
    10261 tactttttgg gggggctatt gctgaatcgc tactcgctct tacaggttct acatacaatg
    10321 aatccaaatt cgggattgac tcttctgcta ttgtctctat ttttgaattt acatcgggtt
    10381 gaatggaatc ggttttgcta gatgctattt ttatattgtc cccagctaat tgctcaaagg
    10441 gttttggaaa acgaaacaaa aaatcatcta ccctaaaggt gctacctggc acaattatgg
    10501 atagtaatgt gtaaagaatt gctaccaaca gcaggaaacc taacgtatgg taaggcttgt
    10561 tcatgaaatg tttaatgcta acggatttta aatgtaattg tttttgttaa atggtgtata
    10621 ttaggtagca ataattggaa agtaattttg cttttaaatt tgataaatag taacttacag
    10681 gatgttataa tggcaatgat aagagtgtta cataatccac gatgcgcaac cagccgaaag
    10741 gggttggagt acctgaactc aaaaaatatc aattttgaag taataaacta tttgtccacc
    10801 ggcctaagtc gcgatattgt gaaagagatt ttgcttaaat caaacctaaa acccattgaa
    10861 ctggtaagga ctcaggagga atactacaag aaatttctga aaggcaagaa ttttaacgat
    10921 gaggaatgga tagagatact tactcaaaac ccaaaacttt taaaacgccc agtagtgatt
    10981 ggtaaatata aagctgtaat aggtatacct gtagaaaata tcgataaagt tttataaact
    11041 tccatagcca tgaaattagg aaatttaatt ttagccgcac ttacaccgct attattctcc
    11101 ccaacagcat tttcgcaaaa tgccgatttg gtaagcatgg agaatgaaat tcttaaaata
    11161 gtgaacgagc ataggaaatc gattggtaaa tcggaattgg tgtttaacga ttatattcgc
    11221 aaagaagcat acattcattc ccagaacatg gcatcgggta aggtaacatt tagccacaag
    11281 ggattcgatg aacgctttaa tcggttaagc ggttttctgg aaattacaac cggagccgaa
    11341 aacattgcaa atggccccct aaacgcaaaa catattgtaa acggatggct tgccagtcct
    11401 ccccatagaa aaaatattga agatgatttt aatcttacag gaataggaat agcaaaagcc
    11461 ccaaatggct catactttta tacccagata tttgtaaaaa gtgctgagaa acctaaggtt
    11521 gtacccaggg agtacgaggc agaactttta cagctgataa ataaccaccg taagggtctt
    11581 gggctacctg ccataaaaaa cgacgaaggt attcataccg aggcactcgg gtactcccag
    11641 caaatggcta caggcaaagt tcctattggc ccaccgagtt tcgacaatcc aattaaatcg
    11701 ctaatgcgtc ggtataaagc atcaaaaatg gtagagctta ttggatatga ttacctcaaa
    11761 cccaaggaac tatttgatgc ctggatgagc agcactagcc agcgcgatat aatagagggt
    11821 aatttcaacc taacgggaat tggtgtttac cagtcagtta acggcaaagt gtttgttacc
    11881 caggtgtttc tgcttcagta accaacctaa aactaaatac catggcaaca ccggaattct
    11941 tttaccagga aagatttcct ttggagaagg atgatactga ttactatctc ctaacaaagg
    12001 attttgtttc atcagcaaag tttgagggcg aagagatttt aaaggtcgac ccggagggtt
    12061 tggcatttct tgcccgcacc gcaatgcacg acatctcttt cttttacagg gctgaacatg
    12121 taaagcaaat tgctgcaatt ctggaagatc cggaagccag taaaaacgat aagtttgtag
    12181 cgttggtatt gctccgcaac gccgagattg cagccaaagg ggttttaccg ctatgccagg
    12241 atacaggtac tgctactgtg tttgccaaaa aggggcaaag ggtttggact ggcgtcaacg
    12301 actacgaggc actttcgcgt ggtatctaca aaacctacac cgaggaaaat ttgcgttact
    12361 cccaaaccgt accccttgac atgtataccg agaagaactc ggggacaaac ctacctgccc
    12421 aaatcgatat ttactcaact cacggcaatg agtacgagtt cctctttata gcaaaagggg
    12481 gaggttccgc caataaaagt atgcttttcc aggaaaccaa ggcactactg aaccccgaaa
    12541 agctggaagc atttttagtt gaaaaaatta agaatttggg tacagccgct tgtccaccct
    12601 accatattgc catagtaatt ggaggaacat cggccgagga tacacttaaa acagtaaagt
    12661 tggcttcggc aaaatacctc gataacctgc caacctccgg gaacattcac ggtagagcat
    12721 tcagggataa agagctggaa gagaaaattc taaaagccac caacagtatt ggaattggtg
    12781 ctcagtttgg cggtaaatac cttgcccttg acattcgaat tatacgcctt cccaggcatg
    12841 gtgcatcatg tccggtagga ataggggtat catgtattgc cgatagaaac ataaaggcaa
    12901 aaattaacag ccaaggtatt tggattgaga agctggaaaa gaatccgggc aaatatatcc
    12961 ccgaccaatg gcgtaagcct gaagaaaacg atgctgtagg aatcgacttg aatcaaccca
    13021 tgcaagcaat tctttcggaa ctcaccaagt accctgtagg cacaaggctt tcgctcaccg
    13081 ggccaataat tgtagcccgc gatattgccc atgcaaagct aaaagagcgt ttagataagg
    13141 gtgaggggtt gcccgaatac gttaaaaatc accctattta ctatgctggt ccagcaaaaa
    13201 caccagccgg aatgccctcg ggttcgtttg gtccaactac tgcagggcga atggattcat
    13261 atgttgaaac attccaatcg cagggtgcaa gcctggtgat gattgccaag ggtaaccgca
    13321 gccaggctgt aaccgatgcc tgcaagaaat atggaggttt ctacctcggg agcattggcg
    13381 gtcctgcagc catactagca caggaaaaca ttaaaaaggt tgaactaatt gagttccctg
    13441 aattgggaat ggaggcggtg taccgaattg aggtggttga tttccctgca tttatactgg
    13501 ttgatgacaa aggaaacgac ttctacaagc aaataaagta gctccttaag gttttcgaaa
    13561 taaagaaggc ccgataaccg ggcctttagt tttaagtatt tttcttttta aatgatcagc
    13621 atggcatcgc cgtaagtgcc aaagcggtat ttctccttaa tggcaacctt gtaagcatcc
    13681 ataacctgat cgaacccgcc aaaggcacaa accatcatta ggagggttga caaaggcaag
    13741 tggaagtttg taaccagcat gttgggtaca gcaacctcgt atggcgggaa tataaacttg
    13801 ttaatccacc cgctgtatgg ctttaggaag ccatcggttg tcacagagct ctcaagggta
    13861 cgtagtacgg ttgtaccaac tgcgcaaacg ttgtgctgct cgcgtttggc cttgttaatg
    13921 atgttggcgg catcgtcgct aatccaaatc tgctccgaat ccattttgtg cttggtcaaa
    13981 tcctcaacat caacagttct gaagttgcct aaaccaacat gaagggtaat ctcggcaaag
    14041 tttattcctt taatctcaag ccgtttgagc agatgcttgc tgaaatgcag acctgcggtg
    14101 ggagctgcaa ctgcaccctc gtgcttggca taaacggttt ggtaacgctc acgatcttcg
    14161 ggtacagcct cgcgctgtag gatatgcttt ggaacgggca tctcgcctaa cctgtaaagg
    14221 gtttccttaa actcctcata ggaaccatcg aaaaggaaac gcagggtacg tccacggctg
    14281 gtggtattgt caataacctc agctaccaga acatcatctt cgccaaaata gagcttattg
    14341 cctattcgga ttttacgggc agggtcaact aaaacatccc atagcctttg ctcccggttc
    14401 agctcacgga gaaggaacac ctctatttca gccccagtct tttccttatt gccgtatagg
    14461 cgtgcgggaa aaacctttgt gttgttgaat actaaaacgt cgtgttcgtt cagatactca
    14521 ataatatcct taaaaatccg atgttcaatt ttcccggttt tcctgttcaa caccattagc
    14581 ctcgattcat ccctgttctc aacaggaaat tttgcaatta aatcttttgg cagattgtac
    14641 ttaaattgcg atagcttcat atgtatagtt ttacttcaat ttacacaaag atataacttg
    14701 ccaccaggtg ttgtcaacag ttttaccctt aatttatagg aatttgcaaa gataatctat
    14761 tattttagag catgtgcacc ttatacggca atatttagct ttggttacct aaatcagata
    14821 gttttttttc cacctcagca attgataagg cttgctcaat tgagaaaata ccactacgcg
    14881 tacgccgtaa ctcaaccaga tgtgctcctg tgcttagggc cttaccaata tcgcgggcta
    14941 aagctctgat gtaggtaccc ttgctacaaa ctatttttat tgttagaaac ggtaaatcaa
    15001 aaccgataag ctcaatgcta tgaatctcga ttggaaccgg ttcaagctta ggatcgacac
    15061 ccttacgagc atactcgtag gcacgttttc cgttgacata ctttgctgag aacgcagggg
    15121 gttcctgcaa ctgccatcct gaaaatgtcc ttaaaacagt ttcaaccagt tcacgattca
    15181 cgtggtcaaa ggggtaaaca gcatcaacat cggtctctaa gtcgaacgat ggggttgttt
    15241 cgccaagctt gattttagca atgtactcct tattgtcggc cgtaagctcc ataacccttt
    15301 tggtagcttt tccggtacaa attaccaata ggcccgtagc caatgggtca agggttcctg
    15361 catggccaat tttagttgat ttgtcgccag tgtatttttt aatcaacgat cgtaacttcc
    15421 ccacaacatc gaacgaagtc caatcgtatg gcttatcgat aagtaatatg gtaccctcct
    15481 taatgctctc gaaacggttt acctccatgg gctaggctaa aaacagtgca actataccaa
    15541 ccagcgcaca gtaaagtgca aaccagtaaa gattcccttt ccgaacaatg cgaagcatga
    15601 atgagcaggc caaaaagccc gatacaaatg cagcaacaaa tcctgcaact acgggcgcag
    15661 taatggtgtt tgaaatatca cctttgataa tatcgagaaa agcagctcca agtacgggaa
    15721 taataaccat aaggaacgaa aagcgagcta cctcatcgcg gtccttgcca agcaaaagtc
    15781 cggtagagat ggtggcaccc gaacgactta agccgggcat aacggcaaca gcctgggcta
    15841 gacctatgac aacagagtcg aaatatccta ctggttttgt gtttttgggt tttactattc
    15901 gggttaaagc aagaagaatg gctgtaacaa gcaacatgga acccactacc aataaatttc
    15961 cctcaaaaag agtctcaatt tggtccttga ataaaacacc tataatacca acaggaaccg
    16021 ctgatattat aagcttaaat acgtaatggg tttggctgtt cagctttaag ctgaagaatc
    16081 ccgaaaataa atcgattatc tccttacgga aaacaagtag ggtgcttaaa accgtagcca
    16141 catgaactgc aatgctaaac tccaagttgt tttcgagttg aactccaagc agagcctttg
    16201 atagttcaat gtgtccgcta ctacttacag gtaagaactc ggttaaaccc tgtataatgc
    16261 caataattat ggcctcaaaa atggtcattt caaatacttt ttaatgaaaa aaaattagct
    16321 atcctttttc tcgcgtttca ttatggcgta acccacaaat gcgaacccaa acagtacaac
    16381 aacgggggca agcgtaatac gtcggaaact gaaaatgtcg gggttgaaca cattggggtc
    16441 agttgatcca ccgcctgcca tgagaatgaa acccagaact attatggcaa agccaattat
    16501 catcagctta tagttcatcc agcttaaggg aaaaagattc tctttttcct gtatctcctt
    16561 tttgggggta ttttttgcag acataactgt taattttatc gggttaatag taaagatcat
    16621 cggtgcttga gcgtaaaaat ttattcactg ccatcagggt tgctaaaaag ttaataacta
    16681 ttccaattgc agtaattgct gcaaatacaa gcacaatgct tggcggatcg aggatggcaa
    16741 gcaaatcggc aacctccttg tttatagcat acagcaatcc tattaacaaa gcaatggcta
    16801 taaaggaagc gtatagggca tgtaaaatgc ttttcagcag gaaaggtcgt cgtataaaac
    16861 cccagcttgc cccaactaat ttcatagtgt taatgataaa tcgcttggag taaacggaca
    16921 acctaatagt attgttaatg agcacaaggg aaatgaaaag catcagtcct gcaaatgtca
    16981 gtatcaccaa gctaattcta ctaacattat cgttaaccag attgatgagt gagcgctggt
    17041 agtcaacctc ctttacctgg gggaaacgct ttaaccaccc ctcaatgcgt gcaatgctat
    17101 cgttattggc gtaatcggct ttaagccttg cctcaatgta tgcatttaat gggttgtatc
    17161 ccaggtaggc aataaaatcc tcgccaagct cattagccat cattttagcg gcatcttgtt
    17221 gtgtgaagta ctgggttgat tttatgtaga tactagcatc gagctgcttt ttcaggtaaa
    17281 gggcatcggc atcggtaact ccctcgttca ggtagatatc gaacccaatg ttttccttaa
    17341 cgtacgctga aagtttcttt gcgctaataa ccaaaacccc gagcaaaccc aaggtgaaaa
    17401 gcactaagga gatgcttatt attgatgaga tataggaact cctcaacctc cctttagttg
    17461 ccctgtttac tgaattagcc atttactcct taaatgtttt atacaaacta aagcttaccc
    17521 aaccaacaaa ggtaagtatt aatagcaacg atgaagccaa atcgatatac attccttttt
    17581 tccacatagg cgggtcaaat acaaactcaa tttcatggtt accagcaggt atttgcatgg
    17641 ctcttaacac gtaatttacc ctgaaatgat cggctggtgt gccatcgagg tacgcttgcc
    17701 aacctttggg atagtaaatc tcagagaaaa cagctagctg agaaactggc gaatcgctcc
    17761 tatacttcag cctattagga cggtattcag taagttcaat cttaccctga cccgatttac
    17821 cctgctcaaa acctctaagg tattgctcaa atcgtttatc gactactgct attgattttg
    17881 ggttgaagcc attcagggcc tcaatctcct catcggcaat attaacaact ttaactgagt
    17941 ccacaaacca tgcatggcca agtgcatcgg ggttataaac tgcaacggga ccttccttgc
    18001 cgggttgtat aatatacttt gtgtttaaca tgttaaacac agcaatatta cccttggata
    18061 gatgaaaatc aattaaatcc tgatagcgac gcagcttggc tccatggtat ccgccaattg
    18121 atttatggaa ataggatgtg gatgcatcgt tgaatgggct aacggtaagg ttaaacacac
    18181 ggaaatttgg atcgttatcg gctagtatct gcttatcggc atcggttggg gtaaaggggg
    18241 cttcaacctg ctggggtaac tcaaagttct tgttatccat aaagcgcttg tttactaccc
    18301 acaaatccac agtaatgagc aggcccagca aggcaaagaa tagtgttggc ttcaactttt
    18361 tcatgtagta aagcgcaatg gtaactgcac cggcaagtac aaagaatagt gagcggaaag
    18421 catcgttcca catcagcgct tgcctatcct tgcgcattac ctcaacaatc tccttgggcc
    18481 aacccagctg attgtcaatt gaaccgctaa aggataacat tcccttgcca agcagcagga
    18541 acagcaggca aattcctcca accaatccca ctgaccattt aaatgatttc caaaaatcat
    18601 cctttgtaac cttgccatca actatttcct taagggcaag tattccaagc aaaggaatgg
    18661 ttaactcaac tataaccagt atcatcgata ccgttcggaa cttgttgtaa aaggggaagt
    18721 aattcaggaa taaatcggta agaaaattaa aatttttacc ccacgagagc attattgcca
    18781 gaacagtaat cccaaacaaa aaccacttaa atctaccttt aaggataaac atcccgaata
    18841 caaaaagaaa aactacaatt gcaccaaggt aaacaggacc agaggttgat ggctgtgggc
    18901 cccagtatgt gggtaggttt tgtgctatgg aactagcttg accataggga acaccattct
    18961 gcctgagaaa acggaatgtt tccgattttt cgccaacatc catggtgctt gaaccgccca
    19021 tcaaattagg gataagcagg ttaaaagttt cagctattcc gtaactccac tgggttgcat
    19081 agtccttgtc cagccccgac gttctattat ccctatcatg ggttaactca gatttgcctc
    19141 tgattgtatc cttgccatag tcgttagtga accataggta ggtaaagtta gcgccaaaag
    19201 ccagaactgc tgccagaagc aaaatcccgg tagccttggt aaacatcctt aaggcttttg
    19261 ccttaaatgc ctcaacaaaa tagtagagat agacagaggc cattacaagt gcaccgtagt
    19321 aggtgatttg cgggtgccct gcattgagat tgagtccaaa agctagtcca aaaactgcca
    19381 aaccagcaaa aagctgtcca cgtaaagcca aaatcattcc agcaatggtt ggggcaacat
    19441 aagctatggc atgaatctta gcgttatgcc ctgctccaat aactatgaga aaataggttg
    19501 ataggccata ggcaattgaa cccacaatgg caagccaagg gtttacccca aaaacaagaa
    19561 gtagaatata aaatcctaac atggttagga agatatagct ggcaggccgt cccaccacat
    19621 ttaggaagct atcgacatgc tttagcaggt ttcccttgta gttagccgaa atgagatatg
    19681 ccggcattcc tccaaacata ctgtttgtcc aaagcgactc ctcgcctgtt tttgccttgt
    19741 aatccaaaat ttctttcgct gagcctttcc acatagcaat atcgtgctga gctagccttt
    19801 taccctgtag ctgtgggtag aaataagcta acggaatggc catgaaaagc agcaaggcta
    19861 ctatatgggg ctttatctta ctaaattttg aattcatgcc ggcaagtgtt tatttatatt
    19921 tgacgtagta ggttagttag cgaaacctta tcgttcatta tctcgcgcag gcgcgatgcg
    19981 ggaacacgct cttgctccat ggtatcgcgg tagcgaatgg ttactgtttc atcgtcgagc
    20041 gtttggtggt cgacggtgat acagaatgga gtgcctattg catcgtggcg gcggtaacgc
    20101 ttgccaatag tatccttttc ctcgtactgg catgcaaaat cgaattttag gtcgtccatt
    20161 attttgcgcg ccatctcggg taatccatcc tttttaacca gtgggaatac agcaagctta
    20221 acaggagcca gggctggcgg tatgcgaagc actacgcgct catcgctggt accatcctcc
    20281 ttagccactt tttcctcagc gtaagcagcc gaaagaacaa gcaggaaagt acggtctaag
    20341 ccaattgatg tttcaattac gtaaggaata tagctttggt tgatgtcggg atcaaagtac
    20401 tgcagctttt tacccgagtg cttttggtgg ttagccaaat cgaaatcggt gcgggaatga
    20461 atcccctcca cctctttaaa cccgaatggg aattcaaact cgatatcgga agcggcattg
    20521 gcataatggg caagtttctc gtgcttatgg aagcggtact tgttgtcgcc aaaccccatg
    20581 gcacgatgcc aacgcatgcg tttctccatc cagaactcat accatttcat ttcctcgcca
    20641 gggcgaacaa aatactgcat ttccatttgt tcaaactcac gcatgcggaa tatgaactgc
    20701 cgggcaacaa tctcgttgcg aaaagccttt cccacctgag caattccaaa aggaattttc
    20761 atacgcccgg ttttctgaac gttgatgtag ttaacaaaaa taccctgagc ggtttcgggc
    20821 ctaaggtaga ttgtgtttgc ctcatcgcta accgagccta gcttggtttc aaacatcagg
    20881 ttaaactggc gtacctcagt ccagttgcgg gtaccggaaa tagggcaaac tatctcgcaa
    20941 tcctcaatta tctttttaac ctcagccagg ttgttctgtt ccagagcctc aaccatccta
    21001 tggttaacgg tggtaatact ttcggaaagt tcaactaccc ttgggttggt ttggcggaaa
    21061 agctcctcat taaaagcatc gccaaaacgc ttgcgggctt tttcaacctc tttatctatc
    21121 ttttcgcgct gcttctccat ccattcctca atgagtacat cggcacggta acgtttctta
    21181 ctatccttgt tgtcgataag agggtcattg aaagcgccca catgccccga tgccacccat
    21241 gttgtagggt gcatgaagat agccgcatca ataccaacaa tgttttcgtt caggcgtgtc
    21301 atagcctccc accagtaacg gcgtatgtta ttcttcagct caacaccgtt ctgcccgtaa
    21361 tcgtaaacag cgctaagccc gtcgtaaatc tcgctcgatg ggaatacaaa tccatactcc
    21421 ttgctatgtg caatcagctt tttgaatagt tcctcctgtg tcataaatgc tttaaatgaa
    21481 tttccagtaa ctcacaaaaa tagttcaatt tctttacatc caaaaaaaat gagttttggc
    21541 aaatatgttt tgactttgta aatggcattt catatattac caaaaatttt gatactgcaa
    21601 tggttcactt aaagcctttc ataacaacca ttacaaaata tcttgtacta cttttagcat
    21661 tcactagcgc ggttcatgca cagtatcttg caaatttcac aaataacaag tcaacaaaaa
    21721 ttgatgaggt aaaaccttta tcacaaatcc ctgaatataa aattaaagca gcccttatcc
    21781 ttaatattgc cagtaacatt gaatggccaa acgaaaggaa cattaaagaa ttcggaatac
    21841 taattgttga cagcgataca cttatataca atgaaatcag aagtgttgaa accagctaca
    21901 agcttaaagg taaacccata agagtaaatc tttcgtcgcc tccagtaagt aaaccaacag
    21961 agtattcaat tatttaccta agccacagct acagcccttt agttaactca atttaccagc
    22021 aaatagttgg taaaggtgtg ctcattgtta ccgatgaagg aactgaacgg ctacttacca
    22081 tggtaaacct ttttctcaac caggaaaaac aaagtgtatc attcgagatt aaccggcaca
    22141 cccttgaaga gaatggattt accctcagcc caaaacttgt tgcccttggt gggagcttta
    22201 ttgatattaa agagctttac ctaaaaacct accaccagct aattgaagag aataaaaaac
    22261 tgcagcaact acaaaccgac ctaacccgct accagcaaga aaaaacagaa tacgaaagcg
    22321 agatcagaac gctaaacgaa agggttaata gattaaatca aaatactgca gttcttgaga
    22381 agcagtatgc caatgcaagt gtttccctta agcaaaaaga tagcctgctc ctaattgcaa
    22441 caaaggagct tgccaaaaaa gttgcagaga gtcaaaaact gcagatagct atcaataaac
    22501 aggtggcatt gataaactcc tcgcgtagcc aactggactc actgaacaaa caaattgttc
    22561 aaacccaaaa agttttagac cagaaacaaa aggagattga ggaacaggac atagttatat
    22621 cggaaaagga gagtatcatt cttaagcagc aaagacgatt ctttattatg cttattgccc
    22681 ttgtaggaat ttcatttact ctggtatttg cgttttgggc ttacagcatt aaacgaaaac
    22741 tcaatatcaa acttgaaaaa caggtagaga tcagaacaaa cgagttaaac ataagccggc
    22801 aacactacca aaggcttttc gaaaattcac ctgtagctat gcttgagctt gacctgtcgc
    22861 tccttttaaa ctatgtaacc tctatcggaa ttcctctgga tacgatgtac gaagttgatc
    22921 gtagtttctc cattgcaaaa gtacgtgagg gcctaaagta catacaagtt gtaaatgtga
    22981 ataatgcagc attaaagctg cttgggtttg aaagtaaaga tgatgctatt gaaaattaca
    23041 taagaaccta ctccgaagaa tcgcttgaga catttcggga agtttacaaa gcattaattg
    23101 aacgacgaca gtttagcgaa tacgaaagta taaggaatac atacgatggc aagaaacttt
    23161 ttgtggtttt aaaatggata gtactacccg gatactccca cgattactcc agagtgttgc
    23221 tatcaattac tgacataaca aagctcaagg aatacgagaa agagttgacc caacaccgtg
    23281 atcatctgga ggaaattgtt caagaacgca cccaacagat tatccagctg aacaaaatgc
    23341 tccaggagtc aaatgaagaa ctactgctta aaaccgagga gttagaacaa accatccatt
    23401 tactggaaga tgcccaaaaa caacttgtac agcaggaaaa aatggcctca cttggtatgc
    23461 tcaccgctgg tattgcgcat gaaatcaaca accccattaa cttcattagc ggtagccaac
    23521 aggctattga atcgctgctc gatgagcttt ggaatagtct gaacatttac cgcgaaaagg
    23581 ccctaaagca ctcgcccaaa aacattacct atgaacttga aaccaataat agcattcagg
    23641 ttgaggaact ttacagctcc ataaagctaa tgcttcggaa tattgaaatt ggtattgaga
    23701 gaactactgg cattatcagg agcctaaaag ccttcgttcg gtccgatagt aaagagtacg
    23761 ttccggtttc aatacccgaa accattgccg atgtgctcaa catgctgcgg ggtaactata
    23821 aggataggat tgagattatt cagcactacg accaggacct tcccaaagtc aaatgttacc
    23881 ctaaccagct acatcaggtt gtaatgaatt tgttagccaa tgccattgat gccataccgg
    23941 gcgaaggaaa aatttacata agcggtcagt actccagctc cgataacaac gttaccattc
    24001 gtataaaaga cactggcact ggaatcccaa gcgatatcca ggaaaagatc ttcgacccgt
    24061 tcttcaccac taaggatgct ggtaaaggaa ctggtttggg gctttacatc acctacaata
    24121 ttataaagtc attaaatggc gatataaaag taaactcagc accaaatcaa ggggctgagt
    24181 ttacagtaac actaacagct tgcgagtaaa tatagtttct caactatttt caaacctttt
    24241 aaaagctgac tcaattatct cacgaagctc atccttcttc cagggcttca tgatgtagcg
    24301 gtacaccaac tcctcattta ttgccctaag catgacttcc gattccataa aagcagtaag
    24361 aagaatgcaa accttttcag gattctcatg tttaatcctc tcaataaaat caatgccgtt
    24421 catgtggggc attttcagat ccgatataat cacattgata ttttcgttct taaggatatc
    24481 gagccccttt tgagccgaat cggctacaag tatggtaaaa tcgttccgga agttaatctt
    24541 aaatagctca aggttaatta cctcatcatc aacgtataat accttctttt ccatggttaa
    24601 cagggttgat tacccgcaaa agtataggta tttgggaaat atgcaagtat ttcccaaata
    24661 ccataaaaac taattcttaa cgcagcgaac agagtatgct gtaccaggag acccttcacc
    24721 aattgaaaca tttcctgaat aactgtcaat tgttacaatc tgaacgcttc ctggtattgt
    24781 atttacccaa aaataggcat actcgccaac atcggtggca gaccaagatg tatcccaact
    24841 agctcttcca gcaccacggg ctgaaaatcc tgtctcattg gttgcaatta caactgaaag
    24901 aggaccatgc agatcataat accaattgaa aatttcagga aatacatctc taaggggttc
    24961 aattagcttt ttcccattta ttaaagaatc aattgcccat tcagcacccg atacaaaagt
    25021 aaacatggcc tcccattcag gctttgttgg tacatgccaa ccgtcggggc aaacattttt
    25081 accgctaagt aatattgcgc catcagaata gtagtttcca aagacagtgg tggaatcagg
    25141 agtaggcaat acataaatat tactaggttc tattggtgta ccatcggagt acttggtggt
    25201 ttttaggttt tcggccatcc atacctgcgt gcctatctta accgtttggt aggtattacc
    25261 gtcggcatcg gcaaccgggg gctctggcaa tccaacattg gttgtcacat tacctgcaac
    25321 caccatatcg ctgtacgata ccatggtgtt tacaatgtat gtgctatccg gggttacctc
    25381 taagtaattc gcagcgctgg acttagcgtt tgttaacccc cctacagcaa agccgccctt
    25441 ggctcccttg gtgttgctgt cgtcaaaggc aaaccggatg ctgtcgggta gtaacgacat
    25501 gtaaacattg gttcccgact tcccgttggt gagaccgcca accgcaaagc ctcccttggc
    25561 cccctttacc actgggctgt catcgataaa gaaccttgtg ctgtcggggt gtatggtcat
    25621 gtaggtgagt gtatcgccct tgccattggt taggccaccc acggcaaagc cgcccttggc
    25681 tccctttatg atatttgtct ggtcaaacag gaactgggca taggagggtt ccagctggaa
    25741 gtaggtgggt actggggctt taccgttggt tagtccgccc acggcaaatc cacccttggc
    25801 ccccttgccg ctgcttccct caacattaaa tcgtaccccg gtttcgtaaa ccgccattac
    25861 aacctgaccc tgcgagttgg taaccgcaaa aatcggcttg tcggggtcat gtccttctcc
    25921 gggtgatact attaccgtac tgtccagaac gcttaccctt gcggtgggcg cccattccac
    25981 tccattggaa aatacggtat tccccaatgg ccccaggctg gaaataccgt tgctggcgtt
    26041 caacgatttt tccgccagaa aggcgtaggg tacagcaaca atctggcttg tacccaggtc
    26101 agtaaaactt tgctgatcct gctttcttac cttcagctcc aggaagatgg gatcgctcca
    26161 gctgataccg ctaaaagtgc ctgcgttaac cgtgcccgca ccaatctcaa aggcaaccaa
    26221 accctgattg gtggtggttt tggtatgggt ctcggagtat aaaacgtcgc cggtggcggt
    26281 tgttctatgc agcaaaacct atagctcaac ctgctggttt gccattacgg ttccatcgga
    26341 attccgcagg actgcctgat aggctatccg ttggggtacc tgtgcgctaa ttacatagcc
    26401 tactagcacc agtagtacca ccattgcctt tcctaaacat ttcatctttg tatagttttt
    26461 agtttatgag taattaaaga acacaagtta tattttaaat tactacttgt caattatttt
    26521 atgataattt gatattaatt cttctgtctt aattattaag cttaatcagc cattaaaaac
    26581 gtgaaagatt ttatcctgtg tatgcattac ttacccaatt atagttttga tttcaaaatg
    26641 atgccattta tttagaatgg ctcttaaaat atcaaaacaa ggcaactgaa cacatagcag
    26701 atacagctaa actctttata ttcggctgtt ctctattaaa aatctatttt tgacgaatgg
    26761 cggttataga ttgatgaatg aaattggttt tacttcttta tctggaaata ccctatcaaa
    26821 tcggtaagtg ctttagctct agcattcagt tcctcagcgc tggctgcaac ttcctccgag
    26881 agcgaggcat tttgttgcgt ggtgttgtta agttcctgca ccgaggtgtt cacctgctcc
    26941 gttccagttc gctgctcgtg ggtggccgcg gttatctccc tgattaaatc agtggttcgt
    27001 attatctcgt gcacgttctg attcataata tccttagcct tgcccgaaat ctccgctccc
    27061 cgttgcgata ccgcaataat ctcgttggcg gcctggcggc ttcgctcggc caagcgcctt
    27121 acctcggccg ccaccacggc aaagcccttc ccatgctcac cagcgcgggc tgcctctacg
    27181 gctgcgttca acgccagcag gttggtctgg aatgcaatat cattgatgat tcctatcttt
    27241 tgggctatct cgttcatgga gtccagcgct acgttggtgg actcaacccc ctcctccaac
    27301 ttcttggaag tggacgaggc tatacgttct gtctgcatgg cattctcgga gttctgctgt
    27361 atgctggcca ggatctcctc catggcgctg gatatctcct ccaacgatga ggcctgctcc
    27421 gaggtaacct ccgataggct ggtggcactc atgctcagct ggcttgccga cgactccagc
    27481 tctcctgcgg cctgtgaaat gccatcaatc acctcgcgta acttatcgga tagattacct
    27541 accgatttac ttaacctccc aagctcatcg ttgtgctgga caaactcagg gtttaccttt
    27601 acggtcaaat ctccatcact tatcctatgt aaatcgtcaa tagcctggtc caaaggcttc
    27661 cgaaccttgc gggctataaa ccacatcacc caaatacctg ctactataac tacaggtgta
    27721 gttatatatg caggaaaaat ttctgggtag atgtacttta agttcaccat aacatcaatt
    27781 agaactcccc aggttacagt tagagctccc attaaaaaga gtatggagtt cttgaaaaag
    27841 taccttatgg tgagtatgcc cacaggtaat gcaattagca ttattacaat gagttcaagt
    27901 atgcccttat tcatggctga aaggttttat gttcaacatt tgctatttag tgggcaatat
    27961 aaaataattg atgggttaag tcaaggaaaa ctacacgaaa aattcatcca ttgcaatctt
    28021 ttcatgtatt gattggctta aacgttggtt ttgatagtta attcgatccc caatacccag
    28081 taatctgtgt atcctgaacc ggtttttaac cgaaacatta catagtagca aatcgagaag
    28141 ttttgctata aaccttggtt taactattcc taccaatgat ttagtcctat cgtacatgga
    28201 caagggttcc ctaaaaccag caccatggtg ataaaccaaa tcggcgtaaa taccaaacat
    28261 taagccatgg ttagttagct gcgatgtgcg ttcaagtggt agccagtcaa tttttaggtc
    28321 gattagaatt ttaaagagat ttccccctac atccgttcgg tgtataccag cagtggtttc
    28381 ccactcgtaa ccctccttcc agtcgccttc aattgatttc cagaacccta ccgtggtaaa
    28441 acagaacgaa gggtgtggtt gaatatcacc aatattttcg gttcgcctta tggcggcaag
    28501 cgggtactgt tttaacgtta aggtgacgaa actatcaatg ggtttaacag gaaaggcatc
    28561 gccatcaata aaaataagga tatcgtcatc gttagcttga agtgaaataa accgggctag
    28621 aagatttaac ttaatggcat gttcggtaat gggttcgcag caggcaaagt agaacttatc
    28681 cttatgttta caggcatcgc cactcagaaa cgcaaaaact ctatatggct ggttaatgtt
    28741 ccttttaaaa aatttgagtt gaatgtcgat ccatttatcg gtttcccagt ggacagttgc
    28801 aatataaatc atacctactg ctttcttata tcacaaaaat aaccaaattt gctttaacta
    28861 tccataatgg taaaatggca aatcccattc aaagcccagc ggttttaata tttactgccg
    28921 aaacgttccc ggcgatggcc ggcgatggcc gtaatgccct gcttgtagca tcaaaactca
    28981 atgagaatgg ctactgtgta aagttaatct gccttaaccc taatggaaaa ctacccaaga
    29041 aggaaaaatt gaatggggtc gaaatatccc gaataaatta ttggtaccgt acaattattg
    29101 gaagatttgc cttaaggtta agccttttcc gatacttaac catcaattgt aatagtagtt
    29161 ccaaatggat aatttttggg gcgatgcccg ggtataaact cattgcctta gtctccaccc
    29221 taaaaggggt tgattgtgtt ttccgctcta cactatgggg ttttgatgat gccgagagtg
    29281 ttgcaggaag tttacatcgg ttctacaccc gatatttatt gagtaggctt tacggctact
    29341 atgcccttaa ctcggcattt gcaaattcat ggagaaacgt ttttggtagt tttaacattt
    29401 tgctgtcagt tcagggtgta gatttaaaaa agttcgatat cgatcataaa aatgggattc
    29461 gccatgagca tcgtggcaaa cttcaaattg ccgagggtaa gtttgtaata ctaatggtgg
    29521 gacatctgat tgaacgaaaa ggctttcctg aaattgcgga ttggcttagt agaattgatg
    29581 aggattttct tctattgcac attggcagca ctgaggcccc cgaatgggat accatgagca
    29641 ggtataacgt ggggatgaaa aatctaaaaa aatacgttga agaaaaatta ggcgataggg
    29701 ttcggttcat gggccgacaa aacgttacaa cacaattcta cctattgtcc gatatccttt
    29761 tggtggcatc gcatgcggaa ggattcccgc caaactcggt taacgaggca ttagcagcag
    29821 gccttccagt gcttaccctg cgaatgccag gcgttgccga tgttattact gaaggtgtta
    29881 atggctttgt atttaccgat gaatctgagt ttgcccgtaa actatcaatt ctcattaata
    29941 accccgttag gcgcaatgaa cttgggcgga atgcccgcgt ttttgctctg gataagcttg
    30001 atataaataa aataattcca acactacaaa attttcttgg cctttaacta gaattcaatg
    30061 tcgtttattt aaaaaaaaat acacgcctgt catcctgagc gtagcaaagg atctctaatc
    30121 gaccccgtga gatgattcgc ttgcgctcaa catgacaacc ctctcatgaa acgagcaaca
    30181 gcaagtatca cagagttaca cgatgtaaca cggtgttaca cagagcgtgt gacgtgatat
    30241 tcacgaacaa cgattcacga atagcgaaga acgctacccg ccccgtaggg gcgtaatatt
    30301 tgtaacacca tgattatacc taaaatgtat agcgatgcac gcaatacatt ttccaaagag
    30361 taaaggtgaa gcgttgcatc gcaacagaaa agcactgcaa agagcgggtt acatgttctt
    30421 tcttgtcttg atacaagaaa gaaccaaaga aaatcaaggc tgaaaagtcc gacccattgc
    30481 cgggtctggc ttttatgcct ctgcttgctt cttccgcttc attccctctc agggaaaagg
    30541 tctggggtga ggtcgtatcc tacattcaac ctatatgacc cctctaaatc tcccctgaga
    30601 ggggagactt tagccctgca ataaggtttc cttattgctt ctcccctctc ctgtcaggag
    30661 aggggctggg gtgaggtcgt tttccacgaa caaccaacga cgcttcccgc cccgtggggg
    30721 cgtaatattt gtaacaccat gattattcct acatgtatag cgatgcacgc aatacatttt
    30781 ccaatgagca aaggtgaagc gttgcatcgc aacagaaaag caccgcaaag agcgatttcc
    30841 caaaaaacaa caagggttaa aggtaaagag acgcttcggc taactcagca tgacagcgtg
    30901 atgtttcgct tgcactcaac atcacaaata accatacctc aacaacattt aattaagatt
    30961 tctgctcatc tatatgttta acaaaatttt atggtattca tgcagcatta tcgttttttc
    31021 ctctgtcttt tgtataaatc attttaaaaa tatactgcca tgagattagg aattttagct
    31081 ttgctgctta ttgcagcaac cgtaaccagc acaactataa gtgctcagga aacccgaaac
    31141 tttgatgcta agggatttga cggaatacga aacagcacat cggctgaggt gcaaatttca
    31201 caatcgccaa actatggtat tgaggtaacc gcaaagagtg aggttttcaa tgaactagag
    31261 atagttgtaa aggagaatgt tttgcatatc aaaagcaagg ataacctaag atttaccagt
    31321 aactggggta aagtattggt caaggtttca tgtccggata ttaaactttt ggctcagaat
    31381 ggctcgggaa atatgattgc actaacgccc ataaaaggtg ccgacgaatt tacaacttcg
    31441 gtaaatggga gtggcgacat attgctaaaa gagatacagg ccaattcggt acaggttagc
    31501 ataaacggtt cgggaaatat taaaacatca gcgcaaggga aaacctatac gctaaaggcc
    31561 aactctaacg ggtcagggaa tatcgatctt gagacattaa cctcggagat tgctcaacta
    31621 aagttgaacg ggtctggcga tgcaaccgta aggtgcaaca aggaatttaa aggatcaata
    31681 aacgggagcg gaagcatcac gctttatggt aatcctctta ttgatgccaa ggttaacggt
    31741 tcaggacgat tagtccgtaa gtaaagattg gataacggtg cacaaaaaat aacggctgag
    31801 gttattcctc ggccgtacat attttaatat ttgggatatg tgaggttcac aatttgatga
    31861 attggtatag gtttgggatg cataaactaa attccccctc ccagaatatg caaaacttgc
    31921 atataaattt gtagttttga tttttattct aagcttttag tatcatgctc agagtggcag
    31981 ttgattttga tggtacaatt gtggagaatg aatatcccca gttgggaaag cccatgctat
    32041 ttgcctttga aacccttaag gaacttcaaa aaagaggctt tgtccttatc ctttggactg
    32101 tgcgaaccgg taagctgctc gaggaggctg tggagttctg ccgccagaat ggggttgaat
    32161 tctacgcagt taatcgcaac caccctgaag agattcttga cccggagact cctcgtaagc
    32221 tcgatgttga tgtttttatt gacgatcgca acctgggtgg atttcccggt tgggataagg
    32281 tgttcagcat gctttgcccc aacgagccct atgaggtttc gcagaaaaag gttttaaaac
    32341 agtataaaac gaaaagtttt gtacgacgta tctttggtat ttaacatcta ttttatgcta
    32401 caaagaattt caattttgct attagttcta gccgttgctt gcacaaatgg ccgaaaggat
    32461 caactgcccg ataccactag tatgccagca gagaatcctt tgcatcttat ccggttcgac
    32521 aagcccacaa gtggttcatt attcacagcc ggcgaaagca ttgatattac aattaaacta
    32581 accgatacta ttcagcccga ttccattgtg ctttaccgta acagcgaacg aattcagaaa
    32641 gttagcaatt tatcgttcac cttacaaacg caagctggta aacccggcac aattcagctg
    32701 aatgctacag catggaaaga tgggcgcagg caaacagcat caatttccat aaagctcaaa
    32761 tcgaatacaa aacctgccag ctacagttac cgaatagtaa aagcctatcc gcacgacccc
    32821 gaagcctata cccagggctt attctaccat aacggctacc tgtacgaggg tactggtcag
    32881 caaggcgcat cgagtttaag aaaggttgag ctggaaactg gaaaaatatt acagagcgta
    32941 aacctaagca gggaatactt tggcgaaggc attgccattc ttgatgataa aatttaccaa
    33001 cttacctgga caaacggtac aggctttgtt tacgatgctg ccacttttaa acccttaaac
    33061 actttcagct ataccacgca gggctggggg ctaaccacca atggaaacga acttataatg
    33121 agcgatggct caaatactat ctactttaga gagccaagtg gttttagtga gcttaggaga
    33181 attgaagtat ttgacaacaa tgggcccgtt aaaatgctta acgagctaga gtacattgat
    33241 ggtaaaattt acgctaatgt ttacctgacc gataggatag taataattaa ccccgaaacc
    33301 ggagtggtag aaggaaatat cgacatgcag ggacttttaa ctactacaca aagaaccggc
    33361 aaggaagatg tattaaacgg tatagcatac gaccccaatg gaaagcgaat ttttgtaacc
    33421 ggaaagctat ggggtaaact atttcaggtt gaattcataa agaaaaagta gatgtcaacc
    33481 cgcactgaaa ccgactacct tggaagttta caaatccctg ccgatgcact ttacggaata
    33541 cactcggtaa gggcaaaaga taattttcct gataccacac aattccatgt tgagtggtac
    33601 caggctatgg gactggtaaa acaggcatgt taccttacct atgctgaaat ggttaaggca
    33661 ctccaggaag aaaatttatt ccgcgatagc aaaggtttta ttccgcctaa cgtagtaaat
    33721 gccctaattg cagttgcagg cgaagtgcat gagggcaagt acttcgacag tttcattgtc
    33781 ccggccatac agggaggtgc tggtacaagt atcaacatga atgttaacga gataatcgct
    33841 aacgcaacac tcatcaggct tggccataat cccggcaact acacggttat cgatcccatt
    33901 gagcaagcca atgtttacca aagcacaaac gatacggtac caacagcact tagggttgcc
    33961 gtggtaaggt tgttaaccct gctcgaggag ctaatcaata acacccgcca ggcagttgaa
    34021 accattgaga aaagttaccg aaactcattg cgtataggat acacgcagct acaggcagcg
    34081 gtaccatcgt cgtacggaca gctgttcagc acttacagcg aagcgctatc gcgcgattgg
    34141 tggagggttt caaaatgctt tgagcgtata aaggttgtta accttggcgg aggagccatt
    34201 ggtacagcag tgggaacacc ccgctacttt gtaatggagg tggttaagca cctgcaacag
    34261 ctaaccggat tcccgtttac ccgcagcgaa aaccttaacg atgccacagc aaacctcgat
    34321 tcttttgttg aggtgcatgc cattctcaaa gcccatgcag tgaatattga gaaaatggca
    34381 tcggatatac gcctgctggc gtcggatatt gcgggcaatg caattaacat tcccaaacga
    34441 caggtgggca gctcaattat gcctggcaag gtaaaccctg taatccctga atttgtagta
    34501 agcattgccc atcgggttta caccaacgat atgctcatct caaaccttgc cggacagggt
    34561 gcgcttgacc taaacgccta cctgccaacc attggacacg ccatgattga ttccataaag
    34621 ctactgattg ctgccaacca agccctagca ggccatttac taaatggcat tgagatcgac
    34681 tctgagaaat cggctcagct actgatgttt aatccatcca taaccacagc cctgcttcct
    34741 attattggat ttaaccgggc tgcaatgctt gcgcaggaaa tgcgtctgaa aaaaattgat
    34801 attttcagcg ccaaccaaaa cctgaaacta attccggaag aaaagctggt tgaattggta
    34861 aaaccaaaca acctgcttaa gctgggattc tcgtttcgcg aggtgattga ttaattctca
    34921 atatctattt aagttttcac aaaacataag cgacggttga aaaatttgtt tgtaaatgat
    34981 aattcaattg gagtaatgaa aaaatttact gaatacatag ttgttgttgc tgttgggttg
    35041 ctttttacct acctgtttgc tggcgatgta gttaaaaata taaatcacag atactttgcc
    35101 caaagcggcg atggtgcaaa ggactactat gccacattct accacattaa gcacgataaa
    35161 agctacatac attccaacag catgaattat ccctttggcg agagtatctt ctttgcgggt
    35221 tcgcagccaa ttgtaagcaa taccgttaag ttcatttcac aaaacatata cgatgttagc
    35281 ccttacacag tagccataca taaccttctg attctgatta gcattgccct cacggttctt
    35341 gtaatctacc tgatttttaa acatcttaat ctacccattt ggtactcgtt acttgtggct
    35401 atagggatta ctttcctttc ccctcaaatt gacaggattg gagggcattt tgccttgagc
    35461 tacaccattg tgcttccgtt attcattctg cttttgctaa agttcgaccg ccaaaaaagt
    35521 attggtctat cagtactcat tctttttgct ggaatgtggg catcaataac ccatggttac
    35581 tttttcactt tctacatggt gatgtttttg gcctactggc tctggcaaaa accttggcgt
    35641 aaggagtttg aatggcgcaa acagtggtta cacgtggtgc tgcaaattgc tattcctctt
    35701 attgttgtcc ctaccctaat tctgtatata gagcccgttg aggatcgaac aacatggcca
    35761 tggggttttc tgttttaccg ggctttccct gagtccgtgt ttctttcgcc gggtcgccct
    35821 tactggggct ggctaaatag ccttgtaaaa atacgtaacc tccagtggga gggaattgcc
    35881 tatgttggat tggtgtctac aatcattttt ttaataggcc ttttcagctt tattaaaaat
    35941 gctgttaata gacgttgggg aaaaaccctg accataacca gtcagccttt aatggatcgg
    36001 cttttctggc tgtcgttttt actcctttta ttctcatttg gaataccgtt tatctttaag
    36061 atggagttcc tgctggaata ccttgggcct attcgtcagt tccgtggtat aggtcgattt
    36121 gcatggcttt tctactacac tatgaacatt gtggcgtttt acctaatttg gcagaaagct
    36181 aaagggtata aacctgttta caggatttta ctggtaatcc caataaccat tttgctctac
    36241 gatgcctggt ttcatagcaa atcgcaaaat cactttatca acaatagcct acctgagctg
    36301 gattatgcct ttcattcaaa cagtaagccc aatatcaata ttgatgagtt tcaggccatc
    36361 atgccaattc ccttttttag cgtgggaagc gaaaactact ggcacgagtc ggcctgcttt
    36421 ggttccatgg taaagatgta tgccttatcg cagctaaccg ggttgcccct aaacgccacc
    36481 tacacaagcc gatcatcggt ttggcaatgc gtggaaaacc tcaacctgat tatggaaccg
    36541 ctccaggaat accccgtact aaaatactac aaccatggca aggatatcct tttatttgtt
    36601 gacccacatt gcgaccaaat aaaccagaat gaacgccgaa ttattaccat atcaacccca
    36661 attgatacac tctgggggta tgagctacga aggttgaaag tggccgacct tgctaagctg
    36721 cccgaggtat accgtaatga gtaccttaac gctacccctg gaatggccga taccatttct
    36781 ggcaattact actttcagga atttgagagc actcccacac ccttagcgtt tacaggaaac
    36841 ggggcgctca agataaaccg acggggttac acaaaccttt actgggggcg cttgccaaac
    36901 aacagctcat cggaaatcac ggtgtcgttc tgggttaacc gcatgaaagc agatattgta
    36961 ccccgcaatc gtattgagat tgttaccggt aaagaggaag gaaagtggga aacctggaat
    37021 gggtttggtt ttatggagaa aacaaaggca ttctggaacg attgggcatt ggtggagtat
    37081 aacctgaaac tcaacaaccc gtccgatttt atttgcattt cggttgctcc attgctggct
    37141 aaaagcggag atgtttacat tgataatgtt cttatcaaac cgtcaaattt caatgtaaaa
    37201 ttcgtcatgg gagattttgt tttactgaat aacaggctgt ttaacaatat ttcaatcggt
    37261 tcagattaga attttaccct aaccatacac ttcacctcgc tacgggtatt ccccataata
    37321 aggtcagggc ctgaccctat agttgccgaa cctgggaagt aggtttgagc atatcgtaac
    37381 cataaatcaa caccctttga gggtgagtat ttaagcatcg ccataattct ggttccatta
    37441 gagtagtagg ctggaaccgt aaaggagtaa agcaaatcgc tctcgtacga gtaaaggcgg
    37501 gtattccatg attctgtatc gaaaatggca aagcgtaacc tgatgctcaa cggtgttttt
    37561 tgcaggttaa tgttgatatc ctgcgctagc aggtatccat attcaccacc ggaaaggcta
    37621 tcggttctat agttcgatat ctccagggtt gacctcaggt ttaccaaagg gttaggctga
    37681 taggtaagct gagtacggaa accttgtcga gtttgggtta gtgttgatgt taccggaata
    37741 ttagcaccag tcatattttt ctcatcctgc ttgtaacggt aacgaaaact taactgaaaa
    37801 tatgtattta gagagtgttc cgaaaggaaa agaaaatctc tccctgccga tggcgagtaa
    37861 accccatagc gcatccatgg gaattggtaa aggtcaatat agcccgatat tttccatcct
    37921 tttgcggcta gcaagcttaa ccccatcagt aatccctttt cattggtagc acctgagccc
    37981 tcagcaaggg cagcagtgta acgggggtta aaggttgtgc tatagctcct ccctaaaacc
    38041 gaaagatgca ctcggttact tagcttcaac tgcccaccgg caagggctgc cattttctgc
    38101 gagaaaatat cgatagcaat ctcaccaaaa aacagatgct gacggtagta gtaatcggca
    38161 tttattccca tagcaagttt ctcggtagaa gcaggcgtta ggctgtaaat gggttgattg
    38221 tttaggtttt caccataaat tctgctgtaa acagcagttg ttcctagcct tagcttatta
    38281 aaggttaggg ttatattact gccggttacc agttctggaa tatttcgtcg gctcgcaatt
    38341 tccgaagccg ttcggtggta accggtttcc ggcaacgatg aaaatgcaag ctcacctgaa
    38401 ctaaccgtat cgacaagatt gccatctatt ttttggtatg aagctagcag gctaaaatcg
    38461 atatgcttga agtttaaagt gaagcctgca cccctcaaaa aaagcgattc gtaagctgag
    38521 gaatgtggca caatgcccct gccccgctta tgtagcccca ttacgttcgc cgattttcca
    38581 aaggttaaac tattccaaaa tgttagccct tgtccaaatt cagcacggaa atcaccaacc
    38641 acaaggcgtt taactttccc aacatccgaa accattgcgt agcctgcaag gtaatcgaaa
    38701 ccggaaggga aggttccgtc gaggagtggc tcaccggcat cgttctcggc tgtaacccca
    38761 atttgcaaac ggtttgccct gtaacggtaa cgggtgtaaa ttgcatactt gctccctaag
    38821 taactgctcg caccgccata atcgctccta tagccaactg gggtttggag ggttgttttg
    38881 aggcgagtga aaatatcgtg cctccccata gtaaaatcgg aaactttcaa tggttttaca
    38941 acctcgccag ccacaacaaa tggagcaagc cgctctacat cagtcaggtc gaatcccgga
    39001 actaactgca actcgtaaac actaagtatt ttgccagtgg aatcgcggta gcttaacaaa
    39061 ctctcaatct gaaaatcggt aagaaaaaat agcttttgca aatcggactt acgggcggca
    39121 ttcaggttca aagggctctc ggcaagttga agtaaatcct caaccagctc cgaataatcc
    39181 tgttcagtat cggagttggc agccatctcc tcaaggatat cggctacaac ctgttgcggg
    39241 tcggttacca gctgagcatt gcttaccaat gccacaaaaa caaaaaggga tagcaaaagg
    39301 tgtttcgcct tcattgggtt ttattatctt ttttgccaaa caaataggca accgatagac
    39361 ttggtgtaaa accaagttgc tgatggtggc ttaagctgaa gtcgatatca aagcctttaa
    39421 cgggtaaccc tactccagcg gtaatcccct cagggcttgc tgctgagtaa cctaatcgca
    39481 aagccgtacg ttccattacc ctatactcaa gtccaaaccg aaaggtgggc gcgcgttggg
    39541 tgtcgtcgtc aatttgaaat gcaagtaaaa tattttgtga gggcaaatag gttacgccta
    39601 agcccattag ggtgggcaaa tcgcctaaat cgtcgaaggt tgagcccgtg ggattaaaaa
    39661 cgtaaaagcc aaaggttagc ttggaggttg gcatataatg aactccaccc tcaacagtaa
    39721 aagcggtggc attgcggtaa tcggcaggta gttgcatacg agtaagagca agccctaccc
    39781 cagcagcaaa acgtttcccc agaaacattc cataccccaa gccagccctg cttgtactaa
    39841 acccgctaaa tcccaaatgt gaaatatcaa caccaaaagt accaggtttt acagggattg
    39901 caacccccag catgcttagg ccaagttctg aaagcataaa ccgattctcg tggtgagctc
    39961 caacccaaaa ctggcgctgg tacgctaacg atgcctgatt ggaaaaacca tcccagttgc
    40021 cctgcatgga aagcgaacag tttcccatag cagccgccgc tccggaaata ggcctgatat
    40081 cggcttgact ataaaccgat agggcttgaa atagtaaaag tagtactata ctaagctgcc
    40141 gaaataattc cataaatccg gtttatacca caagttaaaa agaaattgaa aaacatgcaa
    40201 ttggaatgta aaaaggtatg tgcaacggaa aagcttattg atgttgattg ttgcaactgt
    40261 tcgaaaaaag gagttaactt tgcactttat tttaatttga taaaccatga aacaggtcaa
    40321 gctaaaagat aaggagttcc ggttaagcta tcccgaggca gaaattcaga acgatatcga
    40381 cttggtagca tcaaaaatca accacgattt tgccaagagt ggcgaagttc cattttttgt
    40441 tagcgtgctt aatggttcct ttatgttcac agccgacctg ttaaaacgga ttacagtacc
    40501 ctgtgaggta tcgtttgtaa aacttgcctc gtaccagggc acctcatcaa ccggtaaggt
    40561 aaatgagcta ataggcttaa acgaaaatgt aaccggcagg acccttgttg ttctggagga
    40621 tattgttgat actggcatta ctttaagtaa gctatacgaa gagctccgta agcttaaccc
    40681 aaaagagata agggtagcca ctctgctctt caagcctgaa gcctataagg gcaatattaa
    40741 actcgattac ataggccgta gcatacccaa cgattttata gtaggctatg gtctcgatta
    40801 cgacggactt ggtagaaacc ttccagatat ttacactcta atttagataa tgacagccta
    40861 aacaattgcc cacctgagct ttcaggtggg tttttgtttt tagttaagga ttgatattta
    40921 tcaaaatcgg ctaaataaac aaacacccag agcaaaaaaa ggtaactttg aaatccgtta
    40981 aacagtttgt aaaatatgga ttcattagtc aatagccatt ttattatacc ctttcttata
    41041 tccatgtttc tggccatcaa tatgggaggt agtggaacag ggccagcatt ttcagttgcc
    41101 tttggagcaa acgtactaaa aaagacctcg gtaccagccc tgtttgggct aatggtattt
    41161 ttaggggcaa taattgcagg caaggcaacc tcagcaacag tgggtaaggg acttgtaaac
    41221 cctgagctta tgaactacgc ccaggtgtca atcattcttt ttgcggtatc catttcgcta
    41281 cttgtggcaa acctttttgg gattccacaa tctacaagcc aatcaacagt actagctgtg
    41341 atagctccag gcatttactt ccatgagctt aattcccgca aggtgttttt tgaagttatt
    41401 cccacatggt ttatcctgcc cattattgca ttcatcttaa gtctaataat tggcaagtac
    41461 atttacaccc cattacggag aagggggtat actatatcga gccgtataaa tgaccattac
    41521 atgctgcgtt gggcaataat ttttgcctcg ctttacgtgg ccttttccat tggtgccaac
    41581 aacgtggcaa atgccagtgg gcccttaacc tcaatgacca tgaatgagct gggcttgggg
    41641 aatcagggcg gaaggctgct tatccttatc atgattatgt caaccatgat agttgccccg
    41701 agctttggca taggcagttc gctatttggt gagaaaatcc tcaggaatac aggaaaggag
    41761 ctcttcctat ttgggcgaat tgaggcagta ataattgcta ttatatctgc aacccttttg
    41821 cttgcagcct cgctctttaa gggcataccc acatcgctgg ttcagctcaa tgttggggca
    41881 atactgggta ttggcgttgc ccgccttgga cctcgaaata tctttagaaa aacagccgtt
    41941 cgtaagtttt tcctgatgtg gcttattgca ccgattattg ctttccttat ttcgtggtac
    42001 cttaccttcc tggccgataa gtacggatac ctttaactta agtacgatga gttaataaat
    42061 tcaaccaggc ttcgtacaaa caaaaaaagc gtacccaatt ggtacgctat atgttattgt
    42121 taacgagtat tctactcaaa catcccacgc atcctttcaa agaatgagcc tccctcatcg
    42181 gggtttgggg taaaacttgg cgattttgca aatttctcaa aaattgcctt ttcctcccgg
    42241 gtaaggtttt tgggtatgta aacattaacc gccacaagca aatcgccgcg ctggtagctg
    42301 ttcacctcgg gcaaaccctt accccttaag cgaagaattt tcccgggttg ggtgccaggc
    42361 tctattttaa tcttcacttt accatcaacg gtaggaattt cagccggtgc accaagaact
    42421 gcgtcgggga tgctgatgtt aaggttgtaa attaggtcgt taccgtcgcg caccaggtta
    42481 gggtgttttt cctcctcaat cattacaagc aaatcgccag ggacgccgcc acggcgggca
    42541 gcattaccct tgccctgaac gttgagctgc ataccctcgg caacaccagc aggtatttta
    42601 acggttatta cttcgtcctc gcgaaccaca ccttcgccat tacatgcaac gcacttgttg
    42661 gttattacct taccttcgcc attacaggtt gggcatggcg aagtggtttg catctgcccg
    42721 agcagggtat tagttacacg ggttacatac cctgaaccgc ggcaggtaga gcatgtacta
    42781 tacgatgaac ctcctgccgc accggtacct ccgcatgatt ggcacgatac atacttgttt
    42841 accttaatct ttttctcaac cccgttagca atatcgctta acgacagctt aaccttaacc
    42901 cgtagatcgg taccgcggtt tgtgcgtcgc gatgaacgtc caccaccgaa gccaccaaac
    42961 ccgccaaaac tgccaaaatg gccgccaaaa atatcgccga actgggcaaa aatatcctca
    43021 atggtccagt cgtgcgagaa accaccaccg cctgcaccgt tactcatacc agcatggcca
    43081 aactggtcgt aacgggcacg cttgttatcg tcgctaagga cctcgtaggc ttccgcagcc
    43141 tctttgaact tctcctcggc atccttatcg cccggattct tatcggggtg gtatttgagt
    43201 gccattttgc ggtatgcctt ttttatttca tccttgctgg cattccgcgg cacacctagt
    43261 atttcgtagt aatcgcgctt ggttgtcata ccctacctat tttactctcc aaccactacc
    43321 tttgagaagc gcattacctt ttcgtgcagc atgtaaccct tttggattac gtcaactatt
    43381 ttgcccttct gggcatcgtc ctgcaccgga attttggtaa tggcctcatg caaatcggtg
    43441 tcgagctctt tgccaatggc atcaatctct ttaactcctt ttgccttaag gaaatcgttg
    43501 aatttattta caattaatga tatgccttcc ttaaccgcat taatatcgtt agccgtttca
    43561 atggccttaa gggctctgtc caggtcatcg ataaccggaa gcaggtcctt gagaacttcc
    43621 tccgacgcaa attttatcaa ttcagctttc tccttgagcg ttcgcttacg gtagttatca
    43681 aactcggcag taaggcgcaa atacttgtcc ttcatctcct ccaactgggt agtaagctca
    43741 gcaaccttgt catcattggc ttgactggcc tcggtttgct gagcatctcc ttgaggagct
    43801 tgtgtattac tatcggttac attactttcg gtactggttg tttgctgatt cacctgctct
    43861 tgctcaatat cctccttttt gtgtttcttg ctcatatatg atgatttttt tacgtttcac
    43921 actaattccc aaccttacat tcaaaatcta ttccagctgt tggtatcggt caaattgaca
    43981 tagaattatc atgtattgat gtcattagta ccttcttcaa gctaagcagc atgacaaaat
    44041 tgtaatgctt ccccgtttag agaaaagctt aacccataaa agctcattat aaccattccg
    44101 accctttgct tgattcggtt cggctacttt gccctcagct taatatcttt cacccgaagc
    44161 tgcagggtga tattgccttt gtaattattt tcatgtattg caaagcaagc gtcaaatggc
    44221 tggccatagt gtattggttt atagtactca gccatctgaa atgcaattgc atcgaacttt
    44281 tgggtactac cttcctgaac cagggttaac ttgaggtgct ccttctcgct gccaaccagt
    44341 cgggcttcgc cgttatcgta cacattttca gccatgaaaa ctggcgacat gtttcctggc
    44401 ccgaagggtt gaaactgctt taacacgttg aaaaacctct ctgtaatatc atcgagccta
    44461 atgcgggtgt caatatcaat ttgtggtgag agcatttcgg gggtaatggt cttgctcaca
    44521 tgctcctcga accgcctttt aaactcctca accctatcaa ccttcatggt aaggcctgca
    44581 gcgtacatgt gaccaccaaa gttttcgagt aagtccgaac atgcctcaat ggcctggtat
    44641 aggtcatagc cctgaaccga tcgtgcggag ccggttgcca aatcgcccga ttgggttaga
    44701 ataatggtag gacgatagta ggtttcaatt aaacgtgagg ctacaatgcc caccacgccc
    44761 ttgtgccacg atgggttaaa aagcactgta gagtagctgt gctgcaaccg cttgtcctgc
    44821 gcaatcatcc ttagcgcttc gtgggtaatg gtgcggtcaa cattctttcg gtcgttgtta
    44881 cacgagtcaa ttagtgcacc aacctcaaag gctgaactgt cgtccgatgc gcaaagcagg
    44941 tcaacagccg ttttaccgga ctccatgcgg cctgccgcat taatccgagg gcctatgcgg
    45001 aacacaatat cgtcaatagc aatcctttgg ttctcaatgc cagctatttt gattatcgat
    45061 tttagtcctc ggcttggatt ggtgtttagc ttttccaacc cataaaaagc taatacccta
    45121 ttctcatcca ctataggtac aatatcggag gctatactta ctgcaagtaa atcgagatgt
    45181 tgatacagtt cctcctccga gatcccctcc tttttgcagt aaccctgtag cagcttaaat
    45241 cctaccccac aacccgaaag ctccttaaac gggtacatgc aatcggagcg tttgggatcg
    45301 agcacggcta ctgcttcggg aattcgttcg ccaggtaagt ggtggtcaca gatgataaaa
    45361 tcgatattgc gaagcttagc atacttaacc ttttccaccg cctttattcc acagtccagg
    45421 gcaataatta gcgagtagcc attctcaaag gcaaaatcga tacctttgaa cgagattcca
    45481 tatccctcat tgtacctatc gggaatgtag tatccaattt ttgagtacct cttgcggaaa
    45541 aaagagtaca ttagggcaac ggcggtagtg ccgtcaacgt cgtaatcgcc gtaaaacagt
    45601 attgattcac ccctacggat agcctgctca atacgctcaa ccgcctcggc catatccttc
    45661 atcaggaatg ggtcgtacag gtcggcaagc gatggcctga aaaagcgttt agctttttca
    45721 taggtatcaa caccacgctg aaccagtagc gaagcaatag tttcgttaac gtttatttgc
    45781 ttagcaaggg aacttaccaa tacaggatcg cccggatcct ttagaaccca tcgtttttcc
    45841 atttctgcat gttttaaaag ttaaacagta cctacgcggt tacatgcgaa atgaaaccga
    45901 tgagcaaagt ggttaaggag tttagcctta acctcttcca tatcaagctg gcggttaagt
    45961 tccacctgca tggaggttac ccccttatca ataaacccgc aagggtttat gtaacggaag
    46021 tagtccaagt tggtgttaac gttaaaagct aagccgtgca tggttacata tcggcttgcc
    46081 ctaacaccaa tagcgcagat tttgcgttcg cgacccttta cgccagggtc gagccaaacg
    46141 ccggttgctc cttcgagcct atcggctaca ataccatact cctgaaggca atcgattact
    46201 acctgctcca gcaggtgaat gtaagatttc agcgttaatc caaatgcctc caggttaaat
    46261 atggggtagg caacaatttg tccggggcca tgataggtta tatcgccccc tcggttgatg
    46321 tggtagaacg atgcattaat tcttttaagc atctcttcgg ggatcagcag gttggcgttc
    46381 tcgccacttt tgccaagggt ataaacatgc ggatgttcgc aaaatagcaa gtagtgcaac
    46441 ttgccatcgc cgttcagctt acgtttggtt acctcgtcaa ataatttttc ctgataatcc
    46501 catgcctcct tgtaatcaac cgttccaaga tcatgaaact ttacgttttg cattatatat
    46561 tgtttttcct atttgcatag caagtcaacc cattaaggtt gattacttac aacacccctt
    46621 aaccaggtgt tgtgccttat cggcgtggta cgatgaacgc accagtgggc cgctttcggc
    46681 aaattcaaac cccttagcta gggcaatctg cttatactct tcgaactcct cgggggtaat
    46741 atagcgttga acgggtaaat ttgtggggcg tggttgcaag tactgtccaa tggtaaccat
    46801 tttgcatccc acagcaagca aatcgtcaag ggtttgaatt aattcatcgc gggtttcacc
    46861 caaaccgagc attatacctg attttgccct aaaaccgcgc ttagcaatat gctcaagcac
    46921 tttcaagctc cgcctgtagc ttgcttttgt tctaacaagc ggggtaaggc gctccacggt
    46981 ttcaaggtta tgcccaacca catcgggttc cgattccagg aaaaggtcaa taagttcagg
    47041 cctcccgtca aaatcgggta ttaaaacctc tatggtggtg ttgggattaa cccttcgaat
    47101 ctccataaca gtactacgcc aatgcccagc tccatggtcg ggcaaatcgt cgcggtcaac
    47161 cgatgtaatt acacagtaac taagacccat taggcttacc gtacgggcaa ccttagtggg
    47221 ttcattggga tcgggaggaa gcggttttcc ggttgcagta gcacaaaact tacacgaacg
    47281 ggtgcatata tcgcctagaa tcattagagt tgcaacccca tttccccagc actcgcctat
    47341 attcgggcac attccactgc tgcaaattgt atgcaaccca tgtttcttaa caatggaatt
    47401 cacctgggca tacccttcgc caccgggtaa ctttatcttt agccattccg gcttccgttc
    47461 atttcccatc cccaaaaatt tttacaaaat aactaaaaaa tttgctctta aacgattaat
    47521 ttggagtaaa agtaaatgtg gtgagcagtg aaccgttaaa gttaaaggat aaaaacactt
    47581 cctatcttta accatcaacc gtaaattaaa aaaagtatca cagtgttaca cagagtttac
    47641 actgtgtttc acagtgtaaa tcagcattat ttgcgtaatc tgcgttctat tttggtttaa
    47701 ctaccaacaa tcaaccaaca accatcaact taaaccgttt cacagtgcaa atccgctcaa
    47761 tctgcgtcat ctgtgttcca ttaccttaca caaacaacaa aaatgtattt actattctgc
    47821 catacgaagc gaaaagtgat cctcgttgtt caagttgctg tattcaatta aacccattgc
    47881 ggttaggtta ataagtattt tttccgcttc ggatactgag ataaagccca acctgctgaa
    47941 atggtcaatg gtgattgttt gatgcgattt taggtaatct attaatgcat tttcgggctc
    48001 tccgaatagt atgttaatgg gtttacggta cttttctgcc ttccagatct taatttgaac
    48061 aggcgaagcc accacgttct catcgtttac acgtatataa gctttataaa ctcctgtttt
    48121 atcaggtgct ttatggggct tgcgtcttga gggttttacc tttacctcca acacggtttt
    48181 gccgtttatt tcccacttaa ccacttcaaa aggcacctcg ggtttacagt atagctgcga
    48241 ggccgcctca accatgtagt actcctcatc ggtattcaca ccggttatgt tgccattatc
    48301 cttaacgcca atgagcaatg tacctcccgc agtattagca aaagctacca acgaccgtgc
    48361 aattttgcgc gaatcggtta tagagtgttt aaaatcgagt gtaagcccct cgccctgcga
    48421 tatcaaacct gcaatataac ggctcacaac tttaggtttt ataggttaag atggcaaaat
    48481 taactctttt agtgacccca cctgtaatgt tcgatagcaa agccaagcat ctcaacaatt
    48541 ctgtcggaaa cggtatcaat cagttcatcc atgtttgaag gcctactgta aaacgatggg
    48601 cttgctggca aaactacagc cccggcctgg gtaacggtaa ccatgttttg caggtgtata
    48661 agattgtaag ggagttcgcg cggcaccagt actagctttc gtcgttcctt aagcatcaca
    48721 tcggctgctc gaactattaa atcgttactt gtgcctgagg ctattcgtcc taatgttccc
    48781 atagtacaag gaactacaac catagcattg tagccggctg aacccgatgc catgggggcg
    48841 aacatatcgt tattatcgta tagctttaca ggaaatccga aagtgatatc gcgatttagc
    48901 tcatattccc aaaccctttt cccgttctcg gaaaaaacta cggcaacctc atcgacccta
    48961 gccgataatt caattagctt ttcaataacc ctcgccgcat atacagcacc actggcaccg
    49021 gtaacagcta caatcacctt ttgcatctat tagaataatt agtctaggat agaacaaagg
    49081 taaagaaaaa aggttggaag ttgacattat tcaaatgtat tcaccctttt taaagtagcc
    49141 attttcggct tttattatta tacattcatg gttacatatt ttgccttttc aaattggtgt
    49201 tcatttactt taccattttg cttaaagcta aaaactgagt taatttaaat aaaactaagc
    49261 gattaggact aaacgttttt ctaccggtta aattgggttt aaatttccac tatctttgaa
    49321 agtaaattag gcaataaaca atgacaaaaa agaagaataa aaccgatgcc ggttccaaac
    49381 caaatcgtat agccaaaaag gagttaaaga aactcgttac cgatttactg ctggccaatc
    49441 ctaccaacat ctataactac aagcaggtat cgaataaaat tggggttagt gacgattata
    49501 cccgtcgtat ggtcaatgaa attctctatg agcttgcgct cgatgggttt gctgaggaac
    49561 ttcagcgtgg caagttccgt gctgttatgc agaacgctct ggctgttggc atactggaga
    49621 tgaaccccga cgggtcagct gatgttataa ccgatgacaa ccgtgagata tttgttcccg
    49681 actttaggct gaatcacgca ctgcatggcg ataaggttca aatatcaatt taccgccaac
    49741 gccgtagggg tttacttgag ggtgaggtgg tgcgcgtgat tgagcgtgcc aaacgcaact
    49801 ttgttggaac catcaacttt gtaggtaaaa acgcatttgt tgtacctgat aacaagctta
    49861 tgccctacga tatcttcatc ccaaaggttg agctgaagga tgtccgaaac gggcaaaagg
    49921 tgattgttca gattaccgat tggccggcaa gggaaaagaa tcccaatgga aaaattgtag
    49981 aggttcttgg cgatcctgga gttcacgagg tggagatgca cgccattctg gccgagtatg
    50041 agctaccata ccgtttcccc gaagagctgg acatattagc cgaaaagatt agcgacaaga
    50101 taaccgaagc cgattaccgt gagcgacgcg atttccgaaa cgtacccact tttactatcg
    50161 atccctttga tgccaaggat tttgacgatg ccctatcggt gcaaacccta cccaacggca
    50221 actttgaggt gggggttcac attgccgatg ttacccacta cgttaagcct aataccccaa
    50281 tcgacaacga ggccattgag cgcggcacat cggtttacct ggtggatagg tgtgtgccca
    50341 tgcttcccga aaggctatcg aactatatct gctcattacg tcctaacgag gaaaagcttt
    50401 gcttttcggc tgtgtttgag ctgaacgaga atgccgaggt gcttaaccag tggtttggcc
    50461 gtacggtaat cctgtcgaag cgtcgattca attacgagga ggcccaacag gttattgaaa
    50521 ccggcaaggg cgatatgagt gaacaaatcc tgctgctcca caagttggct caaaaactcc
    50581 gggcagagcg attcaaaaag ggggccatcg catttgaacg cgttgaggta aagttcgatc
    50641 ttgataccaa gggaaaaccc cttggggttt acttcaagga gaataaggaa tcgaatcaac
    50701 tcattgagga gttcatgctg cttgcaaacc gcagggtggc tgaatttatt ggaatgcgaa
    50761 aggatgggcg caatgagctg ccttttgttt accgcattca cgacaaaccc aacgaggaga
    50821 aactgaatgc tttccgttcc tttatcaccc gttttggtta cagcatttcc ggtactaccg
    50881 ataagcaggt tagcaaatcg ctaaaccagc ttatggaaaa ggtgaagggg cgcccagagc
    50941 aaaatcttat tgaaaccctg gccttacgct ccatggccaa agcgcgttac tctaccgata
    51001 acattggcca ctacggattg gcctttaggt attacaccca cttcacatcg ccaatacgcc
    51061 gctatcccga tatgatggtg catcgcctgt tggcacacta cctaaacaat ggtaagccaa
    51121 aggataggga gcaaattgaa atgctttgta agcactccac caacatggag atcaaagcaa
    51181 ctgaggctga aagggcttca attaagtaca agcaggtgga gttcatgcag gataagctgg
    51241 gcgaaacctt tacaggtgtt atatcgggcg taaccaattt tggaatattc gttgagctaa
    51301 ccgattctca gtgcgaggga ttggtttcca ttcgcgattt agacgacgat tactaccgtt
    51361 tcgatgagga ggagtacgcc ctggttggtc aacgtacagg ccgcaagttc accttaggcg
    51421 atgaggtgga ggtggaggtt tggcgcacta accttgccaa aaaacagctc gattttaagc
    51481 tggtgggcat gaagggtaga agcagtaaac ctttgaaacc tcgtaggagt caaacgaaaa
    51541 gtagtcctaa aaatccaaaa gtggcatcaa gcagaaaggg acaacaaaag gccaagaaaa
    51601 aggagaaacg tcgtaaacgc taaaacatca aaatagatga aacgatttat cgttattgca
    51661 ctggcttggg cagcatttgc ctgctcaacc aaaaatgagt taacacctgg ctcatggctt
    51721 ggagtaatcc aaatggactc aattcctggc aggctggatg ttccctttaa ctttgaggtt
    51781 aaggttgata atgacaaggt tcagcttacc gttcgtaatg ccgatgaact tattgaaata
    51841 accgaggttg aacgcattgg cgattcgctt tacgccaagt tcccaacctt taccagcgag
    51901 ctggtaatgg ccgtaaacga ttcgctgggc ggctactact atcccaaagg ggtaaaggat
    51961 ggccgcaggt ataagtttta cggaattaaa ggggaaactg accgtttccc atggtttacc
    52021 gaacaaccta agttcgatat taccggacgt tggtggttta ttgagaatcc gggtacgcct
    52081 gactcaaccg ttatggtcgc cgaactcaaa caggaaggtt caagggttac tggaaccata
    52141 ctcagcacta ccggcgatta ccgctacctt gagggtaagg ttgctgggaa catgttctac
    52201 ttctcaaagt gcgatggcgc tcaatccatt atagtaagag gcaaaattac cgattctgcc
    52261 accatggtaa acggtctcat atcgggtagc ccacgctggg cttcggcctg gcgcgcctta
    52321 cgcgacgata aagcccaact ccccgaggcc caaagcctgg tttgggtgcg caagggttac
    52381 acaacctttg agtttgctgg aaccgaccta aacggcagtc gtgtaacctc caacgatgaa
    52441 cgcttcaaag gcaaagtgtt agcagttctt gccggcggaa gctggtgccc aaactgcctt
    52501 gacgaaggcc gtttctacaa aaccatgtac gaaaagtacc gcgataaggg ctttgaggtg
    52561 gtttcgctct gctttgagga taaaaccttt gaggctgccc aacccaagat taaacgtttt
    52621 gcccagagca ttggagccag ctacaccttc ctatacgttg ccccccgtgg acgcgaacag
    52681 cgcgactcag tactctatgc ccttgagggg caaatggcct accccacaag catgatattg
    52741 gaccgcaacg gcaaaatacg ccgggttgaa accggattct caggccccgg taccggtgag
    52801 cattaccaga aatttgccca cgaaaccgag gaactcatag ttaaactact tgaagaaaaa
    52861 tagttatgaa ctgaaaaggt ggtgggtaaa accccataca actaacccat tttaaacact
    52921 ggaatatgaa tgatttactt gcatttggtt tactaggatt tacctcattt tttacgttga
    52981 ttaatccatt gggcgccatg cccatattta tgacaatgac tgcagagctg aatcctgccg
    53041 agagaataaa aactgcccga aaagcttcaa ttgtagcttt tcttacaata ctcttttttg
    53101 catttactgg tcaattactc ttcaggtttt ttggaatttc agtaaatagt ttacgggttg
    53161 ttggtggagt aatatttttt atgatgggaa tggacatgct ccaggcacga ttaacccgag
    53221 taaagatagc cgaaaacgag gtgaaaaagt acgtaaacga tatttcaatt acaccgctgg
    53281 ccattcccat gatatgcggc cctggagcaa tcactaatgc tattattcta atgcaagatg
    53341 ctacaacaat tgataagaag atggtattag tttttagcat ttttctggtg atgcttttaa
    53401 cctttttaat actctatagt tcctcaaaga ttataaaagc gttgggcgaa acaggtaata
    53461 atgttctaat gaggcttatg ggtttaattg taatggttat tgctgttgaa ttcttttttg
    53521 ctggcatcaa accagtactt atcgaaatat ttgggaaata aaggcttaca accaatttat
    53581 tgccaagcaa caactggaac tcttaactcg tacgtgtctt gagaagcaag tgaacg
//
LOCUS       DAOSAL010000008        51007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_509_length_51007_cov_52.416451, whole genome shotgun sequence.
ACCESSION   DAOSAL010000008 DAOSAL010000000
VERSION     DAOSAL010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 51007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 51007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51007
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_509_length_51007_cov_52.416451"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            329..1435
                     /locus_tag="PK049_02235"
     CDS             329..1435
                     /locus_tag="PK049_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOK60404.1"
                     /translation="MVLSLDSVNEVLSTVLHPETSKDIVSMGMVQGLNVEGSKVSFTL
                     KLTKSNDPFASSLKKAATKAIQQKFGENVEVDILVVADVAPKVSPLKQKASISKVKNI
                     IAIASGKGGVGKSTIAANLAVAVAKTGARVGLIDADIYGPSIPKMFNMEHTQPEIVKE
                     EGMELIVPVENYGVKLLSIGFFVDPNSALVWRGPMATSALKQLIHQGAWGELDYLFID
                     MPPGTSDIHLTLVQEVPVTGAVIVSTPQDVALADAVKGISMFTSPSINVPVLGLVENM
                     AWFTPEELPNNKYYIFGKEGCKKLADRMKIPLLGQIPIVQSIREGGDYGEPVAIHASI
                     TGKAFDELAKNLIERVEWRNRELPPTQIVNVQHK"
     gene            1456..1704
                     /locus_tag="PK049_02240"
     CDS             1456..1704
                     /locus_tag="PK049_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOK60405.1"
                     /translation="MSKEQVVAKVEKAIEVIRPFLQNDGGDISLVEVTDDYIVKVKLH
                     GACGSCPYSIMTLKNGVEQAIIRDVPEIKEVVAVNLNF"
     gene            1935..3383
                     /gene="sufB"
                     /locus_tag="PK049_02245"
     CDS             1935..3383
                     /gene="sufB"
                     /locus_tag="PK049_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HOK60406.1"
                     /translation="MAEEQDKILEEVTGSEYKYGFVTNVETETIPKGLSEDIIRMISE
                     KKGEPDWMLEFRLKAFRHWQTMEMPKWAHLEIPEINYQDIIYYAAPKQNAKLNSLDEI
                     DPEIKATFDKLGIPLDEQKVLAGVAVDAVMDSTSVKTTFRETLAEQGIIFCSMSEAIR
                     EHPDLVRKYLASVVPVTDNYFAALNSAVFSDGSFCYIPKGVRCPMELSTYFRINAANT
                     GQFERTLIVADDDSYVSYLEGCTAPMRDENQLHAAVVEIVALENAEVKYSTVQNWYPG
                     DKDGKGGIYNFVTKRGICKGRNSKISWTQVETGSAITWKYPSCILLGDNSYGEFYSVA
                     VTNNRQQADTGTKMIHIGKNTRSRIVSKGISAGHSNNSYRGLVKVLKGAENARNFSQC
                     DSLLLGDKCGAHTFPYLEVDNPTAVVEHEATTSKIGEDQIFYCNQRGLSTEDAVGLII
                     NGYAKEVLNKLPMEFAVEAQKLLQISLEGSVG"
     gene            3479..3889
                     /locus_tag="PK049_02250"
     CDS             3479..3889
                     /locus_tag="PK049_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004345183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOK60407.1"
                     /translation="MNSSYKSSVFTDLIVWQKAHKLTLLVYSITQKFPHEEIYSLTSQ
                     FRRSVISIPANIAEGYKRKGRLDKLKFLNYAQGSLEETRYYTILSKDLGYIDLSTFEL
                     LDESINEVSFLLNSYSSTIAKNDDKSSTTSSNFL"
     gene            3960..4712
                     /gene="sufC"
                     /locus_tag="PK049_02255"
     CDS             3960..4712
                     /gene="sufC"
                     /locus_tag="PK049_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HOK60408.1"
                     /translation="MLTIKDLHAKINDKEILKGINLEVKAGEVHAIMGPNGSGKSTLA
                     SVLTGRELVEVTHGSVTFNGKDLLEMSPEVRAREGIFLSFQYPVEIPGVSMVNFMKAA
                     VNEHRKYRGLEPLSASDFLKMMRERKELVEIDSALTNRSVNEGFSGGEKKKNEIFQMA
                     MLEPKLAILDETDSGLDIDALRVVANGVNKLKRPDNAVIVITHYQRLLDYIVPDYVHI
                     LYNGRIVKSAGKELALELEEKGYDWIKNGENA"
     gene            4740..6107
                     /gene="sufD"
                     /locus_tag="PK049_02260"
     CDS             4740..6107
                     /gene="sufD"
                     /locus_tag="PK049_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HOK60409.1"
                     /translation="MSDKTIATPILEELVSLYQNNLKLITEGSADILNRHREQAMQHF
                     RELGIPSQKNEKYKYSRIEPLFAHEYQKHFAPKRIAFNVDDIFRCDIPELDTHLALVL
                     NGFYLPKGEKLTTLPNGVIFGSLAEAAIKFPNLVNGRYNSLAENKTDGLVALNTAFAQ
                     DGVFIYIPRNVVESKPIQIINLLMSDVNQLVQYRNLIVVEEGAQANVLICDHTLSPKR
                     FLSNVVTEIFVGNNAQLEFVKMQNEHNDAAQLTHTYAHQQADSRLVTNVLSLHGGFIR
                     NNLAVTLAGEGADAQLYGLYLTDRTQHTDNFTFIDHAVPNCTSNELYKGILDDQATGA
                     FNGKIMVRRDAQKTQAYQSNNNLLLTADARMNTKPQLEIYADDVKCSHGATVGQLDNE
                     ALFYMRARGIGEREAKLLLMFGFAHEVVKRISIDTLRERIDDMVNKRLRGELSRCHNC
                     PMHCC"
     gene            6204..6557
                     /locus_tag="PK049_02265"
     CDS             6204..6557
                     /locus_tag="PK049_02265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOK60410.1"
                     /translation="MDYKELDVWKSSIELAKLVYHFTRQLPSNEEYGLSHQMRRAAVS
                     IPSNIAEGIGRNTSKETIQFLFIARGSIFELETQLILCKEIFKVDTHDYDALFQKLQN
                     CKMLINGFINYYQKK"
     gene            6562..7809
                     /locus_tag="PK049_02270"
     CDS             6562..7809
                     /locus_tag="PK049_02270"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HOK60411.1"
                     /translation="MFNTNNEQRTTNNLLSFRADFPILSREVYGRPLVYFDNGATTQK
                     PLCVLQTIERFYREINANIHRGVHKLSEESTREYENAREVVRKFLNARKTSEIIFTSG
                     TTASINLVAYSFGEVFVRQGDEVLVTEMEHHSNIVPWQLLCERKGATLKVLPFDSNGE
                     LMLNKLPELLTPKTRIVAVTQVSNTLGTVNPIAEIVRIAHSQNIPVLVDGAQGVKHGK
                     IDVQQLDADFYAFSGHKIYGPTGIGVLYGKEEWLERMVPWQGGGDMIATVSFAKTTFN
                     ELPFKFEAGTANYIGAAGLATALNYYSSIGPDIATAYEDELLKYATTKLKEIDGLTIY
                     GNAPEKAAIISFLLNGIHPYDTGMILDKMGIAVRTGNHCTQPVIDHFGIDGTVRASLA
                     FYNTFEEVDRLVEALHKVKEMFG"
     gene            complement(8472..9746)
                     /locus_tag="PK049_02275"
     CDS             complement(8472..9746)
                     /locus_tag="PK049_02275"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HOK60412.1"
                     /translation="MYELENESFRGRSKNGLKGAFTRDRKLTINNLIILVINFKSSIQ
                     RELDSFFKAIAKSDFKIREVTKGAFSQARAKLNPWAFQRLNEVAVNAFYEKANYDVWG
                     GLRVVAVDGTRLVLPNHPSVIEEFGQHQFGPKADSPRSLAMGSMLYDVLNQITIDAQL
                     APYASSERDLLMKHLGHVEEGDLLLLDRGYPCFWLLFLLKARKIEFCVRLKDDWWLQV
                     KDFTRSDEKERLVTFSLPKKDVKKLSEYPEMLGQTITCRLVKVELENGDIEILCTSLL
                     DDQYYTHKDIEELYHYRWSEEEAYKLLKSRVEVEAFSGKTATAVKQDFYAKVFLMTLC
                     AAYAHPIEERVTAEYKADKERKHDQKINRTNAISMTQDILIGVFIRKQFKAALEAFDD
                     IVQNTREIIRPNRKVPRNKKPKRLYYMNYKRL"
     gene            complement(9911..10240)
                     /locus_tag="PK049_02280"
     CDS             complement(9911..10240)
                     /locus_tag="PK049_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60413.1"
                     /translation="MGLGGGGSLTLEHYYNQGLQAKLTCIASKELPKFPSKIKSIHCN
                     VPKKYDSIDDWSNYYIALPDEVKSVFDSNEHFIILVGLGGLTGTLLGKSIFKLLNNRN
                     ANSMSFS"
     gene            complement(10353..11282)
                     /locus_tag="PK049_02285"
     CDS             complement(10353..11282)
                     /locus_tag="PK049_02285"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOK60414.1"
                     /translation="MSKRESIARYNLIIKKLRKQPSSFSEISDYLAFESDLQEYNFNV
                     SKRTFQRDIEDIRSIYNIDIQYDFSKKVYYIDFDEQPEVNERILEAFDTFNALNISDR
                     LSNYIHFEKRRPQGTGNLYSLLHAIKNKVQISFSYQKFWENTSTQRVVEPYALKEFKS
                     RWYLLARDLKDKKVKSFALDRLTQLVITKKQFVYPIDFDVNEYYRHCFGIIGPNAKEP
                     QEVILTFSRFQGNYIKSLPLHETQQIEFENENELRIKLKIFITHDFIMELLSFGENVK
                     VIKPESLVNSIKEVYNKALNLYKSNQNEKGKSD"
     gene            complement(11288..12958)
                     /locus_tag="PK049_02290"
     CDS             complement(11288..12958)
                     /locus_tag="PK049_02290"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOK60415.1"
                     /translation="MEQNIKFRLLESIGKVYEQSKDCKLDSAFFEKVDVELKHLSDYF
                     KVSRNQSLIVAMVFALNYKGDTVDLKDLIEYFDCNPMKLLEFSDDFDVLYSKGIFKKQ
                     KSSHRVRLALSNDQFTINEKITEAILNNKPMPVIEREGFKDVIELLEKFHKLGEQRDS
                     EELSTFELFAQAKELISSNLHFPLIKKINDYQFSIEDTYLLLYLIWETITGNETTDIG
                     RATESIFDNPSERVKYVQKIMSNENKLISQNHLEIVEANFFSYTEMKLSESSVKMLQE
                     LGLKLFANIKKRDNIIEPAKINSKTLYFNEFERKQLDLLKTLLQEDNFKKTQNRLQSK
                     GLPKGITALLHGSPGTGKTETVFQVAKDTNREIVKVDISQSKSMWFGESEKIIKRIFT
                     DYKAYAKDCERMPILLFNEADAIITKRKENSTSNVSQTENAIQNIILEELENFEGIFF
                     ATTNLAKNLDSAFERRFLFKIEFQKPDISVKAKIWNSKLPSLSESECEILANRFDFSG
                     GQIDNIVRKVEIHEIIHGITVDFNSILDFCKTELLSKNRGVRIGFTKA"
     gene            complement(13018..15186)
                     /locus_tag="PK049_02295"
     CDS             complement(13018..15186)
                     /locus_tag="PK049_02295"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HOK60416.1"
                     /translation="MKIERIEKIANNMKDIDGKVLANELVERLNSSSFWELLTEMTKI
                     QIPIIQRDFAQGRENEKTNKIRSLFLESIIEAVESEKGSLELDFVYGNVEKDVFQPLD
                     GQQRLTTLFLLHWYIALKTGELPNTKKHFIKFTYETRISSREFCNELVDNGEFLGIGE
                     KISEKIYDSKWFFLSWKKDPTIKAMLVMLDAIEERLLSKNDEELKIFWQKLTSENPPI
                     LFHFKQLNDIGLTDDLYIKMNARGKGLTDFENFKAKFEKHIKKNVFEKELELSENNKE
                     KWKELTEKTFSHRIDTVWTDLFWKHRGEDNIIDNEMIKFIAGIAINNYAQNLEILENS
                     EDELRTRKILEEKKEKNITDEAVKRERIERRITALFNNPADVSPYDFPTKESFEYLKK
                     CFDVYSKNQNDELLPANVTLWDFCKTKKVKINSDTEIDNNLFIEFIKKNETTYSQRVL
                     FYAQTQYLVNLENFDSESFSNWLRVVRNIVQNATIDSAYAFIGAIGLINELSSGCENI
                     YEFLSSTTLKSGFATKQIEEEKLKAALIKSDNNWKSQIFKAEDDIFLKGEIKFLLDFS
                     KVGEEYKIQEFTSISDKFLLLFESKDDLLRKALLTQDDYPVWDGRTSSLGWAHRYSLL
                     NTENEWKDAFRKKSDKFINAVYKLISNSFNLSDDRITILKNVIQNATPLNDYRSHLIK
                     NDSLLRDCYHKRICLNEKKTILYVLEKTRVVGENYKEIKV"
     gene            complement(15183..16877)
                     /locus_tag="PK049_02300"
     CDS             complement(15183..16877)
                     /locus_tag="PK049_02300"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HOK60417.1"
                     /translation="MSCLTEKTIGEILENKFFIPSYQRGYRWTERQVEDLLNDVWDFI
                     TKTGKKDNEWYCLQPVVVKKTNNHYEVLDGQQRLTTIFLILKHLERFIESEKKSFEIE
                     YETRNTESSNSKLFLQSIDLKSEKESKENIDYFHIFQAYQKIKEWFQTKANQGYNSIS
                     SKFVTPFLESTKVIWYEVSSEKDAIEIFTRINMGKIPLTNAELIKALFLNSSNFPDSD
                     TEEIRLRQLEIASEWDRMEYSLQNDEFWYFINNSDNELPTRIEFLFNIMKNIDGVKNG
                     NDQYSTFRFFSEKFKSKSKDEIDTNWKEIKRIFQTIEEWFTDRELYHKIGFLISANSD
                     ITELLKEVKNKQKQEFRKLLDIKIAEKVKCENLEELEYGKHSDLIRKILLLHNIQTML
                     NNKNETIRFPFERYKKEKWDIEHIHAIATKVKVKFEDQNTWLFYNFVKTEQHTNLELN
                     SQIENAIKGNQIAEDDFQSIVEYVLGDDDNTIRNLCLLDRGTNRSYKNDSFKNKRNKI
                     IENEKNGTFIPVCTRNVFMKYYSKELKDLEIWNENDRADYLDDLKRVVYNFKSQVL"
     gene            complement(16882..17358)
                     /locus_tag="PK049_02305"
     CDS             complement(16882..17358)
                     /locus_tag="PK049_02305"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOK60418.1"
                     /translation="MDCFTAIDFETAQGYRWSICQVGLVRVEHGKITNEVNILVQPPN
                     NYYWDRFIDIHGITPDDTAKAPTFDKVWHKIEPFIKEQTVVAHNGLSFDFPVLNKTLE
                     YYGMQAPDYEIYCTYRIFKDNLASLCNFYKFHLDHHDALSDARACAELFLIHLQNK"
     gene            18234..19010
                     /locus_tag="PK049_02310"
     CDS             18234..19010
                     /locus_tag="PK049_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOK60419.1"
                     /translation="MPFAQKIEDAIERFYSAITVGVNHHKVGILSTIVVHLLLIVVFL
                     FLKIETRKEYYGSTIEMEFEEPKEEAIVQQKLEPTLPPDVLKPEYETEAIRNFAVDAS
                     KTDLNAGLSDEKNTDADELYREANQVYERMQQNRELYEQSQKDIEANIPNTPEKTVPK
                     SKEGQYKGPTVVSYYLLGRKALHLPVPSYKCELGGQVVVNIEVKPDGRVADAVIDRAN
                     SVNDDCINQAAIQAAMASIFTSVSGTARQRGSITYLFVPQ"
     gene            complement(19016..20230)
                     /locus_tag="PK049_02315"
     CDS             complement(19016..20230)
                     /locus_tag="PK049_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HOK60420.1"
                     /translation="MNSPKFSEFPHAKGLLVQEERIEYKKKYKQLTIGIPKEVDPTES
                     RIPLTPEAVEILVQNGHQIIIEKDAGRQANYSDMEYSEKGARIVATASEVYQSDIVLK
                     VAPLTPKEYEYLRENQSVLSSLHLNNHTGDYLKSLIQKRVTAIAFESISDADGEFPFV
                     RAMSSIAGSTSVLVAAEYLSNVNKGKGVMLGGLTGISPTEVVILGAGTAGEYAARAAL
                     GLGATVKIFDNSIKKLMDIQNILGQRLFTSIYHKQVLDKVLKTADVVIGTLAPDETNL
                     SYLVSEDQVRLMKRGAVIIDLGIDRGGCFETSEIRDHSNPSYVKHGVIHYCVPNITAR
                     VARTASIAMSNVFVPLMLQIAQSGGLLSQLKEDSGLRHGVYLFNGILTNEYLGKLYDI
                     PSRDINLLMAAF"
     gene            complement(20344..20769)
                     /gene="tsaE"
                     /locus_tag="PK049_02320"
     CDS             complement(20344..20769)
                     /gene="tsaE"
                     /locus_tag="PK049_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOK60421.1"
                     /translation="MTTIEIKSIQQIDEAAQRVLNAIHGKNIVCFYGTMGAGKTTLIR
                     AICKALGVADVVTSPSFALVNEYTDRNGKPVYHFDFYRIKRIDEVYDFGYEEYFYNPE
                     ALCLIEWPELIEQILPTQGVVNIQIAVNADGSRTLQISD"
     gene            complement(20771..22321)
                     /locus_tag="PK049_02325"
     CDS             complement(20771..22321)
                     /locus_tag="PK049_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004383804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PglZ domain-containing protein"
                     /protein_id="HOK60422.1"
                     /translation="MQPTILWVDDEIDLLRPHIIFLEQKGYHVLTASNGHDALELVSQ
                     NPIDLVFLDENMPGIGGLETIPKIKAIKSALPVVMVTKSEEENIMDMAVGAQISDYLI
                     KPVNPKQVLLVVKKFIHGKELVTEKQLADYRNEFRLISDLISAAKTHLDWTEIYRKLA
                     MWQVKLSGNPDSNLKQMLEMQFEEANQVFGKFVKANYLQWFQTQGDKPLLSPSVLKSK
                     VFPIISSGSKVLLLVIDNLRYDQWKTIEPIIAESWQVSSEDTYFSILPTATQYARNAL
                     FSGLMPLEIHKTYPDLWVFDEEDEGKNLFEKQLLIAQLQRLGLNTNFTYIKTDTLKGI
                     AGGLNIKEFLGSNLAVLVYNFVDVMSHSRTDSEMMRGLAADESAYLSLTRSWFLHSDL
                     KEFLDLASSQGVQVVLTTDHGAIRVKNPVKVVGDRATSTNLRYKLGRSLNYNPKEVFE
                     IRKPEEAHLPKPNISSSFIFALGNDFLAYPNNFNYYASYYRDTFQHGGISLEEMIVPL
                     VTLNPIKR"
     gene            complement(22326..25004)
                     /locus_tag="PK049_02330"
     CDS             complement(22326..25004)
                     /locus_tag="PK049_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60423.1"
                     /translation="MIKSSYTRLLLLLGIVLLLGVWSCSTQKNTFVSRNYHNLTSYYN
                     YYYNAYDSYKSGTYKAEKGINYNYTLPLPVLLIGESQVPGIVSSDMERTLNKCTMLLA
                     RHSINVKPARTKNTLSPKEKEFMSLNEYVKWARESWLLVGKAHIWKQDLTKARSTFEF
                     VIRQFPGLPIWFEAQVLLARVAILEGDFVDAQDRLKSVESNRKRPRNKEFNHLLSSTW
                     AYYYQKQQQPDAMLPHLRKAIELAPKRNDKIRYTYILAQTLQQVGKNGEALVAYQKVL
                     RLNPAYDMAFSARIQMIALSQKQGDELKRELIKLSKDAKNADYLDQLYFTLGNLEKKD
                     GNIDKAIEYYTLSAKYSKSNSNQKGMSYLVLADYYFSKNDYTRAQAYYDSSYNSLDNT
                     YPEIERIEQKAKYLNSLVENITTIKTEDSLLRVAAMPKEERDALIKAIIANIEVEEQR
                     ARAMEQEERNQSLLYQQTQRYRTENTQEGGKWYFYNQASLSYGQSEFQMKWGKRKLED
                     NWRRKNKRVLSSDEVTVAGTGNASGQEPQKQLSPKTPEYYLVNLPFSDSLKQLSVEKI
                     KNAMLRVAEIYEVNLSDYNEAKAAYLKLAQRFAEQDIAANAYYRLYRMASQYNRQDDA
                     ALFRSTLITNYPKSPYAILLSNPAYFEQLSKQQNEGEALYQKAYELYSKGQLSEALSI
                     VQGGLAKTKNTPLEPKFMLLEALCQAKTAELKVFKTSLSNLAANFPKTEEGQLAEVML
                     KQVEQRELQLISGQTDLDIAPVTPQTEIQASIYSATDGEHMFVMLVPKRSNINQTKFN
                     IISFNVDEFLDADLNVANQPFTDYIEMITCTGLKDKQTAIKYFKMISKEPKVFAGLKP
                     TDYQMFVISIDNFARFLSDKSIPDYLNFFKANYKTE"
     gene            complement(25125..26006)
                     /locus_tag="PK049_02335"
     CDS             complement(25125..26006)
                     /locus_tag="PK049_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60424.1"
                     /translation="MKAQGIARVAIVITFAYLLVSCDGSSKKKIELLTKENEALRNEA
                     RIKDSTINQFFAFMGEIEQNLSVIKQKEQTISKNAIAGSELKPDVRQQIDEDIQTINM
                     LMDKNRQTIAALRKKLKEANLKVAEFERMIANVTQQLEEKEREIEQLKENLTQLNFTV
                     TQLNARIDTLMEEKVEMSVKIQSQTEQLNTAWYAIGTRRELIDNKVIDKTGGFLGIGK
                     SSKMKAEFNQNYFVRIDITKTVSIPLSAKKATVVTTHPKESYTLTTNEKGIIEELKIT
                     DTQKFWSVSKYLVIEVE"
     gene            complement(26524..26682)
                     /locus_tag="PK049_02340"
     CDS             complement(26524..26682)
                     /locus_tag="PK049_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60425.1"
                     /translation="MGAPWVELPRDTIRHAYGVTHVVSLSKPTAMFHPRPFAGSGFYA
                     LSYANLCC"
     gene            27325..28266
                     /locus_tag="PK049_02345"
     CDS             27325..28266
                     /locus_tag="PK049_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK60426.1"
                     /translation="MHLLEAKGIEKRYSNHLALDDVSISVPRGSIYGLLGPNGAGKTT
                     LIRIINQITGPDRGEVLFDGRHMQPNDIYRIGYLPEERGLYKKMKVGEQALFLAQLKG
                     LSRAEAMKRLKYWFEKFEIQAWWDKKIEELSKGMAQKVQFMVTVIHEPELLIFDEPFS
                     GFDPINVQLVKDEILELKRKGTTIIFSTHNMASVEELCDHITLINKSRNILSGQIDEV
                     RQSYGSNVFEIAFRGNPDSLRQKVEGRFELLKIDTDNSLPKAQVRLKSQSDANSLLQE
                     LIPAVEIVSFNPIVPSMHDIFIQVVKEYNQKHGLVIS"
     gene            28292..29641
                     /locus_tag="PK049_02350"
     CDS             28292..29641
                     /locus_tag="PK049_02350"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOK60427.1"
                     /translation="MNKIFLIMQREFMTRVRKKSFIITTLLSPIFFAAIAILPSYLAS
                     LEVTEVRTVAVVDNTKQFIDVIPSTEYIKVTYVTDTPAEKVKENLNETGYYALLVIDS
                     TSTPNQAACTIYSQKQPAIDVMQHLENTLEKEIEARKLKTYNIDNLDKILADVKTSIS
                     IKSIKVSQTGEEKESNTLIAMAIAYIMSFLMYMMVLITGNQVMQGVIEEKTNRVVEVI
                     ISSVKPFQMMAGKIFGMASVSLLQILIWVVMTAALAGVGINIISSKMTTKPATEQMQA
                     MAQADPQMAQVQEQATAVANSEGMSFLTALKNQNFPLLIGGFIFYFLFGYLLYAAMYA
                     AVGSAVESITDTQQLTLPITLPLILAIIVMISGIKSPDGPLAFWFSLIPFTSPVIMLT
                     RLPFGVPTWQLALSATLLVGTFILITWLAAKIYRVGILMYGKKYTWKEMIKWISYRG"
     gene            29650..30246
                     /locus_tag="PK049_02355"
     CDS             29650..30246
                     /locus_tag="PK049_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60428.1"
                     /translation="MLTIEQLIESYDKPGTVILLEGKRAVLPTDQHKLIEVGKLLAGS
                     SKHIVFRSGNAKGADYFFSQGVSMVNPSRLQQVTPYRGHRTKETLGRQTVSLDDVDLA
                     SEPNVIFHTKNSSNVSQLIDSFLSLGDNALTVKARYLLRDTIKVVGTSKLQPATLAIF
                     YDDLSKPKTGGTGHTMRVCEKNGIPYFDQRVWFKWVEQ"
     gene            complement(30252..30803)
                     /locus_tag="PK049_02360"
     CDS             complement(30252..30803)
                     /locus_tag="PK049_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60429.1"
                     /translation="MKRNTQFILLFALFFTSCNNIVFNKPQPVDGKIITNLNNAFPGI
                     FISSDNDTLTITRNTISLGSGNKFTVTGTLGKNLDVREYSSGFVVNFSDSVKGRLVWI
                     AYIFKLSNDSLYISFSDFDPNKLDEVEKEIGNIVPFEKIKDENKENSKIILKPSNSRE
                     FDKLVSAGIFNKTISMKMEKPKP"
     gene            complement(30810..31739)
                     /locus_tag="PK049_02365"
     CDS             complement(30810..31739)
                     /locus_tag="PK049_02365"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOK60430.1"
                     /translation="MIKDITSTAATIAIAMLLPCLSFSQNFEADSVFTWQLVETNNIG
                     SQSTLKFIPRAGVTPTGWDFGDSNTSTEVAPTHTYSYTSWDDSVTVTLNYTLNGESLS
                     HSREIPVSPAFFFVFPDRNLGRLASYKRIFLNAYRIPNHPDSLGNLRFTWTVDGSPLA
                     GNAFDDVALDQWPNIYYTFTTAGLHQVKLDVRNSNSGQTAEFTQTIRVLPDFTSGKVP
                     LENIPNVFTPNSDGVNDNFIVPTSGTAWFDFRIFSRSGGLIYKAESSVLVWDGKNNQG
                     TDMPEGVYYYVIEDLSGQYESAKGFVYLFRGKK"
     gene            complement(31787..32890)
                     /locus_tag="PK049_02370"
     CDS             complement(31787..32890)
                     /locus_tag="PK049_02370"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOK60431.1"
                     /translation="MNQLVRYIIIGFVVALVAFLVWYFSSIVAYILISAVLSLMGKPI
                     VDFISSIRIRNWSPPRFLGATIALITIWILFIAFFRVMIPLVIAQFNELGSIDVQSLV
                     ASFAEPINSIQQFIQNYLPASAQGFVLQDFLITKISGIFTVSMITNAFSSTANLLISL
                     VIALFSISFITFFFLKDDTLFFKSVILLFPEKYEMQITRALVSINKLLRRYFVGIVIE
                     STAIMVLDTIGLTIIGVRFQTAVVVGLIAGIMNVVPYVGPLTGTVLGTLIVLATQLHL
                     GFTANMLTFTILVVSVFLITQLIDNVVFQPLIYGNSVKAHPLEIFIVLLVAGSVAGIL
                     GMLLAIPSYTVIRVFAKEFFNNFRVVQKLTEKI"
     gene            33234..34703
                     /gene="cysS"
                     /locus_tag="PK049_02375"
     CDS             33234..34703
                     /gene="cysS"
                     /locus_tag="PK049_02375"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOK60432.1"
                     /translation="MNQIAIYNTLTRKKEVFEPLNPPHVGLYVCGPTVYGDPHLGHAR
                     PAITFDLLFRYLKHLGYKVRYVRNITDVGHLENDADEGEDKIAKKARLEQLEPMEVVQ
                     YYTDRYHSAMKLLNVLPPSIEPRASGHIIEQIELVKKIIDNGFAYERNGSIYFDVVKY
                     NKKHNYGILSGRNIDDLMANTRQLDGQDEKQNPFDFALWKKASPQHIMRWPSPWSEGF
                     PGWHLECSAMSTKYLGEPFDIHGGGMDLLFPHHECEIAQSVAANGKQAVRYWMHNNMI
                     TINGQKMGKSLGNFITLEELFTGTHRLLEQAYSPMTIRFFMLQAQYRSTLDFSNEALQ
                     AADKGLARLMAANRQLDRIKPSATSSVRVDLLKEKVYEALNDDLNSPVAIAVLFDWVR
                     QINLLAEGKETINDSDLHELKSFFRTVIFDILGLVDEHTSGSKHAELTAKLVEMLLSM
                     RMEAKQRKDFATSDRIRDELAKLGVIVRDRKDGFDWELE"
     gene            34751..35140
                     /locus_tag="PK049_02380"
     CDS             34751..35140
                     /locus_tag="PK049_02380"
                     /inference="COORDINATES: protein motif:HMM:NF020360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HOK60433.1"
                     /translation="MDIRWKQRFDNFEKAFLRLKDALRNDSPSELEKAGVIQYFEFTF
                     ELGWKTLKDYLQEMGVEARFPRDTIKEAFTNGLIQDGDLWLDMLEKRNQMSHTYDEFT
                     ANQSFQMVRDEFIHGIEQLYTKLKDLR"
     gene            35140..35454
                     /locus_tag="PK049_02385"
     CDS             35140..35454
                     /locus_tag="PK049_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007660189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOK60434.1"
                     /translation="METFGISEKSYLLIIDVLKSYPEVETAIIFGSRAKGNYKNGSDI
                     DLALIGESLNSKIILDIAGILNEGIPIPYHVDVVNYHSISTPELKEHIDRVGKEFYKK
                     GC"
     gene            complement(35475..36464)
                     /gene="trpS"
                     /locus_tag="PK049_02390"
     CDS             complement(35475..36464)
                     /gene="trpS"
                     /locus_tag="PK049_02390"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOK60435.1"
                     /translation="MDIVLSGIRSTGKLHLGNYFGALRNFVRMQHENKCFFFIADYHS
                     LTTHPTPEDLHGSVKQVLAEYLACGLDPEVATLYIQSDVPQIPELYLLLNMNAYKGEL
                     ERCTSFKEKVRKQPDNINAGLLTYPTLMAADILIHKATKVPVGKDQEQHLEMTRTFAN
                     RFNRMYKVDYFPEPVAFNFGEDLVKIPGLDGSGKMGKSEGNAIYLSDDPETIRKKVMK
                     AVTDAGPTQPNQPKPEAIQNLFTIMKAVSSPDTLQYFEDKYNACEIRYGDMKKQLAED
                     IITFTTPIRERIEAILADNDYLRKVALMGAEKARESASKTIRDVRHIIGFREF"
     gene            complement(36496..37566)
                     /locus_tag="PK049_02395"
     CDS             complement(36496..37566)
                     /locus_tag="PK049_02395"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOK60436.1"
                     /translation="MMTRKRIGIWILLFSVALATLALASRPNPTKESDKEKPAKGTAI
                     IITGAAAKIAQEAALLESLYERGELNDVVFISGASSGALNAVMLNAVLEKKLTWKEYR
                     ELFGDIKNDNVFRTDKKGLPVSNEPLRKLLEEIVVNRLGYKTLADLPFPTAISVVSLR
                     PSPLSEGTYRFCNRKINRESDSTLNLVDVLMASTAYPLVFPPSRINGIKAIPDIPYYD
                     GGIGDDRVPFQALIEFENYRKQPVERVIIISRKRDTDMTLADELKPFGYELKTKLNNG
                     LSPDVLTSLGFWRRLEHIKKRYPELAAKTLVFVPSYRYSFPMFDFNNLKEQYELTYQW
                     AKQNKPIPLNEFLKQKPTLNKP"
     gene            37744..38607
                     /locus_tag="PK049_02400"
     CDS             37744..38607
                     /locus_tag="PK049_02400"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOK60437.1"
                     /translation="MKHIIVPIDFSKESLNGLRLAIIFANRFDSMVQMVHVQKYPTDL
                     TPGQFEEENKKIEKKLEALVEEYSTKMSNSANLSYIIKKGKIYREVVNQAHAFEDSAI
                     ICSTHGASGFEEFFIGSNAFRIISATQCPVITIRHGAMPRDIQKIILPIDVTADTRQK
                     VPITTEIAKVFDAEVHVIAVATMQTDEIDLKLNAYTKQVCSYLKEHEVRFQKASLRGD
                     NITDITIEYALDVNADLISIMTEQNIALSDFVLGSQAQQMLSRSPIPVLSVTPKEIFI
                     MGSFKTYGSGY"
     gene            complement(38610..40097)
                     /locus_tag="PK049_02405"
     CDS             complement(38610..40097)
                     /locus_tag="PK049_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60438.1"
                     /translation="MNEKFFERWGATFVATLLLLIYILGISNLSTIDGWGYAADIVNS
                     SNLLRPHHLLYTITGFYWVKIWHFFLPNAETIYLLKLMNALCASVTAFILFKLLRLVK
                     VDTIQTTIYTIIAGLSWGFLRFSIDNETYTIPVMLAIWASYLFLKAESSPKISNMVYS
                     GLLSASACLYHQIMFFWWIALAIGTIRKLNRRDYKPLLFLAPAVIVPAAYILAMLSMG
                     IDINFTSFFRFIFSDYYTGNASIELNSTILMVGIINLFRTFFQMHGYINYLLAQSWLW
                     WIPLVVAGIAFAFFSFWFVRYTKPTRLEKFHKIHLLALVLQVGFAFMAGGNAEFLAMV
                     PFLTIITATSLSIKRLATVCFAISVSVWNISFGVLPQKIYQADGSDLVIKSITQNTNE
                     RRAYILTDAPKVINRLEYLQIQKPLIVKEVNIEPQKIYATLDSLLLQGYTIYTDCINQ
                     PRVTSRATLITDVSLEWDKLEGYKTTSIDSLEMLGGKVYVTKISR"
     gene            40204..41601
                     /gene="asnS"
                     /locus_tag="PK049_02410"
     CDS             40204..41601
                     /gene="asnS"
                     /locus_tag="PK049_02410"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOK60439.1"
                     /translation="MRNSERTKVKLLLESGVVGAEVAAMGWVRTKRGNKNVAFVALND
                     GSTIHNIQVVFDMTRFTEEQLKGVTTGACIAVRGLLVESPGSGQRVEIQANYLEVLGE
                     ADATTYPLQKKGHSMEFLREIAHLRPRTNTFGAVLRIRHAMAYAIHKYFNDKGFFYIH
                     APIITGSDAEGAGAMFRVTTLDLENLPRTEDGKIDWNEDFFGKEAKLTVSGQLEAELG
                     ALALSEVYTFGPTFRAENSNTPRHLAEFWMIEPEMAFYDIQDNMDLAEDFLKYLIRYA
                     LEHCADDLEFLNKMYDNELIDRLKFVIENPFERLTYTEAIKILEAANHKWEFPVFWGA
                     DLQSEHERYLVEKHFKKPVILTNYPKEIKAFYMKQNDDGKTVRAMDVLFPKIGEIIGG
                     SQREEVYDKLLSRIHELHIPEKDVWWYLETRKFGSAPHSGFGLGFERLLLFVTGMTNI
                     RDVIPFPRTPKNCEF"
     gene            41608..43065
                     /gene="rpoN"
                     /locus_tag="PK049_02415"
     CDS             41608..43065
                     /gene="rpoN"
                     /locus_tag="PK049_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOK60440.1"
                     /translation="MLKQRLQQKLLQKLSPQQIQMIKLLEIPTMLLEQRIKKELEENP
                     ILEEVEQTEPQTEPEIVLPDDNQEGNDDEFSIEDYMNDDDIPSYKLYSSNASPDDKRE
                     EIPFSTGISFHEHLENQLGMADLTEREKQLGFYLIGNIDEDGYIRRKLTAIADDIAFS
                     LGIETNEDELERILYVIQEFDPPGVGARDLQECLLIQIKAKEQQNEITKIAYKILKYH
                     FDEFIKKHYDKIASKLNLSDEKLKLAIDEILKLNPKPGGSFTDPYDQSAQHIIPDFIL
                     DYEDGEFRLNLNSRNVPELRISKEYADMLESYSYNKEFSNSQEREAIAFVKQKIESAK
                     WFIDALKQRQNTLLTTMQAILDYQKEYFRDGDESKLKPMILKDVAEKTGLDISTISRV
                     ANSKYIQTHFGIFPLKHFFSEGMQTDSGEEVSTREIKKILAECVDNEDKRNPLTDEEL
                     MEILKGKGYPIARRTVAKYREQLGIPVARLRKELN"
     gene            43080..43685
                     /locus_tag="PK049_02420"
     CDS             43080..43685
                     /locus_tag="PK049_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60441.1"
                     /translation="MLQRLSLILSIILHPILATTYIFIVIFSSNSFVAFLPVRFKVML
                     TVFVAINTIFLPLLLIVLLHRLRIIHDYTLKASRDRVFPLAISVLPYLFTIFLFSRLQ
                     VPFVLIKILQAGIYTLMISAAISYFWKISLHLTGIGGISGFLLASALQGNQAAVPIFI
                     VSILLSGLLASARLIKGDHNPTQVYTGFLTGFTLVFLIFFK"
     gene            43833..44693
                     /locus_tag="PK049_02425"
     CDS             43833..44693
                     /locus_tag="PK049_02425"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOK60442.1"
                     /translation="MAAKKKVKIFKNLWNKYRLSIFNEENYEEVWQLRLSRMNVIVFF
                     GGFSILLIALVSVLIAFTPLREFIPGYPDSQTRKGYVKNALKVDSLERVVAQWQRYYE
                     DINILLNGGEPLTGRQTVIDTVKPTAKVELSPSEEDSLLRMQIESDEMFNLSVGSSTT
                     KKSNIPFFIPPVKGIITNKYNPSKNHYGVDLVAGPNEGVLAIAKGTVILSTWSLETGY
                     TIAIQHEEGFLSVYKHNSKLLKRQGEFVNSGDVIAIIGNSGELTTGPHLHFELWHHGA
                     TVDPAKYIKF"
     gene            44702..45874
                     /locus_tag="PK049_02430"
     CDS             44702..45874
                     /locus_tag="PK049_02430"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOK60443.1"
                     /translation="MKRIAILGSTGSIGTQALDVIREHPDIFSVELLVAQNSWELLVQ
                     QAIEFQPNTVIIANQSHYQRVKEALANHPIKVYTGKQSIVESVVSSEVDMVLTAMVGF
                     AGLEPTLSAIEAGKAIALANKETLVVAGELVTKAAMQKRVPIIPVDSEHSAIFQCLVG
                     ERSPIEKIILTASGGPFRNHSLDQLMHVSKADALNHPNWCMGQKITIDSASLMNKGLE
                     VIEAKWLFGLNPEQIEVVIHPQSIVHSMVQFIDGSIKAQMGLPDMKLPIQYAFTFPER
                     VSSGFPRMDFSMSHTLTFEKPDTNRFPSLALALESLRAGGNMPCVLNAANEVAVDAFL
                     REQISFMAIPKVVEGAMQAIPFIGQPTFADYIQTDSETRIKSVELINKNIIKWRYS"
     gene            45859..47178
                     /gene="rseP"
                     /locus_tag="PK049_02435"
     CDS             45859..47178
                     /gene="rseP"
                     /locus_tag="PK049_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOK60444.1"
                     /translation="MEILIKAGQFFLSLSILVILHELGHFLFAKLFKTRVEKFYLFFN
                     PWFSLFKIKRGETEYGVGWLPLGGYVKISGMIDESMDKEQLKQPPQPYEFRSKPAWQR
                     LLIMIGGVLVNVILALFIYSAVLYTWGEDYLPTKSLKYGVSCDSLATELGFRNGDKIL
                     YVGGKEIENFQQIAHDILLNENRDVTVLRGTDTLNIKIAEDYVAKLVKSPIIFFPRIP
                     FVVDDVKSGEPAEKAGFIKGDRLLALNGQSAQFFDEFKSEMQRSKGKTVRVTVERNGN
                     PIDLNVDVPETGIIGVYPVADLGKFFELSHREYSFFHSIPAGVKKGFATISSYLKQLK
                     LIFSPKTKAYESVGGFITIGKIFPSTWDWQAFWSLTAFLSIILAIMNILPIPALDGGH
                     VMFLLYEIITGRKPGDKFLEYAQLVGMAILLALLLYANINDIVKLFR"
     gene            47190..47363
                     /locus_tag="PK049_02440"
     CDS             47190..47363
                     /locus_tag="PK049_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004293781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOK60445.1"
                     /translation="MLTLFIGGGELLVIAIVILVIFGASKIPIFMRNLGRGVGEFKKG
                     IKEAENQEDKEKE"
     gene            47469..48578
                     /locus_tag="PK049_02445"
     CDS             47469..48578
                     /locus_tag="PK049_02445"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOK60446.1"
                     /translation="MSFIDAIENLPIFVPTNQTDTARQKPDLYFECQLAEIGRNSPID
                     FDYNPHVRRYIEIFTIERREQISKMIGLANYYFPLFDDVFSKYQLPLELKYLAVVESA
                     LNPLAISPTGAVGLWQFKINTSKMFNLQVNNFVDERMDPLKSTEAAAQYLQYLYRTFN
                     DWSLALAAYNVGPGPIRNAIERANGERNFWKLYPLLPESAQNYVPAFIAAAYVMNNYH
                     EHGITPAPVALAQNQTDTVWVNKPLDLNLMAKAIDIDINIIRFLNPTYRYDYIPEGSS
                     PQVLRLPADKTGLFIRKSKELYLNTSKPKILPALAADSTLTRKVYTVKNGDSLHKLAM
                     QFGCTLQDIYRWNPGLDSTITAGQNIVIWVNPKNL"
     gene            48645..49640
                     /locus_tag="PK049_02450"
     CDS             48645..49640
                     /locus_tag="PK049_02450"
                     /inference="COORDINATES: protein motif:HMM:NF027451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4837 family protein"
                     /protein_id="HOK60447.1"
                     /translation="MKSRWIIILAAAVALSPSCKKGSKGERSLLPNVTGSANELVVIL
                     PKALHSDSLGKLYSDILTEDVPFLPQSEPRFDMVMIPPDAFTDIFKPHRNIIFNDIVK
                     GAKEAKMSLKRDVWAAPQTILYISGPSYKSIYDLLLKERDKVVSIFEQAERDRVIQNA
                     ITYEEKPIREALEKKYGVSMYFPKGYRLNFDHDTIAWVSYETPLTSQGIILYTFPYTA
                     KNTFTVDYLLAKRNEFVKTISGPTQGSYMTTSSVIVPQLTDMMFKNRYYGVMRGLWDV
                     YKHPMGGPFISHITVDEERQRVIVAEAYVYAPSKEKRNYLRQVEALLYTMEIIKK"
     gene            complement(49725..50570)
                     /locus_tag="PK049_02455"
     CDS             complement(49725..50570)
                     /locus_tag="PK049_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60448.1"
                     /translation="MKPVVHLLTVLLLAISVSVQAQESFIVTRINGVVTNNKTGTQLK
                     AGDVLQPSDKVTFDSFDSYVISIGQNMGRYMIKLHEPPAPETIQQLSAMVKDIAVPTK
                     RRSLMAERYRPDETTVSDLRSYFGNDKFTIIGDNIAVPINPQSYPIDENKFVVFYYRV
                     NNNPVSKKIGYENTTLFIERDKLVNTNAGVITQNEIPSVAVYLYERNTKTSEEITKFN
                     LAFVDKETVTNEFMTIIPILVKQKMGKDEIKKYLIEYFYDFYGATDSRSIKAFVDELV
                     DTAIK"
BASE COUNT        14460 a        10475 c        10674 g        15398 t
ORIGIN      
        1 attatttttg tccatacaca taagtttgtg tagacatttt tcaggacggg tcacacattt
       61 aacgtttaac gtttaacggt tcacgtttca cggaataaaa cttcctctaa cttcctttaa
      121 ctccttcaca ttctttctat tctgactcat tcccccctaa agtcacacat ttaactttta
      181 acggttaacg gttcacggtt cacgtttaac ggactaaatc ttaaaactcc ttttcccctt
      241 catcaacctt ttacaacttt agctgtttaa ctttcgatgt ttcaatttaa aagctaactt
      301 tgcggttaat ttaaactaaa taaaaactat ggttttatca ttggattcgg ttaatgaggt
      361 gctttctact gttttgcacc ccgaaaccag taaggacatt gtaagcatgg gaatggtgca
      421 ggggttaaac gttgaaggca gtaaggtatc gtttaccctt aagctaacaa agagtaacga
      481 cccattcgca tcatcgttga aaaaagctgc tactaaagcc atccagcaga agtttggcga
      541 gaatgtagag gtagatattt tggtagttgc cgatgtggca cccaaggtat cgcccttgaa
      601 gcaaaaggca tcaatcagta aggtgaagaa tatcatagcc attgcctctg gtaagggtgg
      661 agtgggtaaa tcgaccattg cggcaaacct tgctgttgca gttgcaaaaa caggagccag
      721 ggttggtttg attgatgccg atatttatgg cccatcaatc cccaagatgt tcaacatgga
      781 gcacacccag cccgagattg ttaaggaaga gggaatggaa ctaattgttc ctgttgaaaa
      841 ttacggtgtt aaattacttt ccattggttt ttttgttgat cccaatagcg ctcttgtttg
      901 gcgcgggcca atggctacca gtgcgcttaa gcagctaatc catcaggggg cctggggtga
      961 gctcgattac cttttcattg atatgcctcc gggaaccagc gatattcacc ttactttagt
     1021 tcaggaagtt ccggtaactg gtgcggttat agttagcacc ccgcaggatg tcgctttggc
     1081 cgatgcggtg aaaggaataa gcatgtttac cagcccaagc atcaatgtac ccgtgcttgg
     1141 tttagttgag aacatggcat ggttcacccc tgaggagcta ccaaacaata aatattacat
     1201 ttttggcaag gagggttgca agaagctggc cgatagaatg aaaatacccc tacttggtca
     1261 aattcccata gttcaaagca tccgcgaggg tggcgattac ggtgaacccg tagcaattca
     1321 tgcctcaatc accggtaagg ccttcgatga gcttgccaaa aacctgattg agcgcgttga
     1381 gtggcgcaac agggagttac cacccactca gatagttaat gttcaacata aataaccaaa
     1441 tcaattatac aagtcatgtc caaagaacaa gttgtagcca aagtagaaaa ggcaattgaa
     1501 gtaatcagac cattcctcca gaacgatggt ggcgatatct cacttgttga ggtaacagac
     1561 gattacatag ttaaagttaa acttcatggt gcttgcggta gttgcccata tagcattatg
     1621 acccttaaga atggtgtgga gcaagctatc attcgcgatg ttcctgaaat taaggaagtg
     1681 gttgccgtga accttaactt ttaatggtat tccagttatt tgattaaagc cccagttggg
     1741 gctttttatt ttactgacat tctgaaatat acaagttgat ataaaacata ccattatttt
     1801 aggtcatttc cttttcatat tcaacttaaa gcaaatatct ttgcacctaa tttagattaa
     1861 atctaattta actgtgaatc gctaaacatt agcctttgta gtttgttgct attttgttaa
     1921 agtgttaaac cgatatggca gaagaacagg ataagatact tgaagaggtt actggttccg
     1981 agtataaata cggttttgta accaacgttg aaaccgaaac catacctaaa ggacttagtg
     2041 aggatataat ccgaatgatt tcggaaaaga aaggggaacc cgattggatg cttgaattca
     2101 ggcttaaggc atttagacat tggcaaacca tggaaatgcc caagtgggca caccttgaga
     2161 tacctgaaat taactaccag gatattattt actacgctgc gccaaagcaa aatgcaaagc
     2221 taaacagcct ggatgagatt gatcctgaaa ttaaggcaac attcgataag cttgggatcc
     2281 cactcgacga gcaaaaggtg cttgccggag ttgctgttga tgcagtaatg gatagcacct
     2341 cggttaaaac tactttccgt gagaccctgg ctgagcaggg aatcattttc tgctcaatga
     2401 gtgaggccat tcgtgagcat cccgatttag tacgcaagta cttggcgtcg gtagttccgg
     2461 tgaccgataa ctactttgcg gctctcaact cggctgtatt cagcgatggg tcgttttgtt
     2521 acattccaaa gggtgtgcgc tgccccatgg agcttagcac ctactttcgt ataaacgcag
     2581 ccaataccgg ccagtttgag cgtacgctca ttgttgccga cgatgatagc tacgtatcgt
     2641 accttgaggg atgtacagca cccatgcgcg atgagaacca gctacacgct gcggttgttg
     2701 aaattgttgc actggaaaat gccgaggtta aatactcaac cgtacagaac tggtaccccg
     2761 gcgataagga tggtaagggc ggtatttaca actttgttac caaacgcggc atatgcaagg
     2821 gcagaaacag taaaatatcg tggacacagg ttgaaaccgg ctcggccata acctggaagt
     2881 accccagctg tattctgctt ggcgataact catacggcga attttactcc gtggccgtta
     2941 ctaacaaccg tcagcaagcc gacacgggaa ccaagatgat tcatattggc aagaataccc
     3001 gtagccgcat agtctcaaag ggtatttcgg cagggcatag caataacagc taccgcggac
     3061 tggttaaggt actcaaaggt gcagaaaacg cccgcaattt ctcgcagtgc gactcgctac
     3121 tgcttggcga taagtgcgga gcgcatactt ttccgtacct tgaggtagat aatccaacgg
     3181 ctgtggtgga acatgaggct acaacctcaa agataggtga ggatcaaata ttctactgca
     3241 accagcgcgg actgtcaacc gaggatgctg ttgggctgat aattaatggc tacgccaagg
     3301 aagttctcaa caagctaccc atggagtttg ccgttgaagc acaaaaactg ctacagataa
     3361 gtttggaggg aagtgttggg taggatggtt atatgagagg aagttaaagg aagttagagg
     3421 aagttagagg aagttagagg gagttgatgg ggttagagga atcaatggct aatagatcat
     3481 gaattcaagt tataaatcga gcgtatttac tgacctgata gtatggcaaa aagcccataa
     3541 acttacgctt ttggtttatt ccattaccca aaagttccct cacgaagaga tttattcatt
     3601 aacatcccag tttagacgat ctgtaatttc aattccagca aatattgctg aaggatataa
     3661 aagaaaaggt agattggata agcttaaatt tctaaattac gctcaaggta gtttagaaga
     3721 aacccgatat tacactattt tgagtaagga tttgggatat atagatttaa gcacatttga
     3781 actattggat gaaagtatta atgaagtaag ttttctgtta aactcttata gttcaacaat
     3841 tgccaaaaac gatgataaat cttcaacaac ttcttctaac ttcctttaac ttcctctaac
     3901 ttcctcaaat tcatttaatt ttaaacaaca attgtagtaa ataatcaaaa gtaaaacata
     3961 tgctaacaat caaggattta cacgctaaaa tcaacgataa agagattctc aagggcatca
     4021 accttgaggt aaaagctggg gaggttcatg ctattatggg gcccaatggc tcgggaaaaa
     4081 gcacattggc atcggtttta accggtcgtg agctggttga ggttactcac ggctccgtta
     4141 ccttcaatgg taaggatttg ttggagatgt cgcccgaggt gcgtgcacgc gaaggaatct
     4201 tcctaagctt ccagtatccg gttgagattc ccggtgtgag catggttaac ttcatgaaag
     4261 ctgccgttaa cgagcaccgc aagtacaggg gattagagcc cctatcggct tctgatttcc
     4321 tgaaaatgat gcgtgagcgt aaggaactgg tcgaaataga ttcggctctt accaaccgct
     4381 cggtgaacga aggcttttcc ggtggcgaaa agaagaaaaa tgaaattttt cagatggcca
     4441 tgctggaacc caagctcgcc atactcgacg aaaccgactc aggcctcgat atcgatgcac
     4501 tacgcgtggt ggcaaatggt gtgaacaagc tcaaacgacc cgataatgca gttattgtaa
     4561 ttacccacta ccagcgcttg ctcgactaca tagttcccga ttatgtacat atcctttaca
     4621 atggtcgcat tgtgaaaagc gccggcaagg aacttgccct tgagctggaa gaaaagggtt
     4681 acgattggat taagaatggt gaaaatgcct aatcaccctg gtgataaaat gtaaacgata
     4741 tgtccgataa aacaatagca acacccatac tcgaggagct ggttagcctt taccagaata
     4801 acctcaagtt aattaccgag ggtagcgccg atatcctgaa taggcatagg gaacaggcca
     4861 tgcagcactt tagggaactt ggaattccat cgcagaaaaa tgagaagtat aagtattccc
     4921 gcattgaacc gctattcgca catgagtatc aaaagcactt tgcccctaaa cgcatagctt
     4981 ttaacgttga tgatatattc cgctgcgata ttcctgaact cgatacccac ttggcattgg
     5041 tgcttaacgg attttacctg ccaaagggcg aaaagctaac cactctgccc aatggtgtta
     5101 ttttcgggag tctggccgaa gctgctatta agtttcccaa tttggtaaac ggaaggtata
     5161 actcactggc tgagaataaa accgatggtt tggtagctct gaatacagca tttgcccagg
     5221 atggtgtttt tatttacatc ccccggaatg tggtggagag caagcccatt cagattatca
     5281 acctgctcat gtccgatgtg aatcagctgg tgcagtatcg caaccttatt gtggttgagg
     5341 aaggcgctca ggcaaatgtc ctaatttgcg accacaccct ctcacccaag cggttcctat
     5401 caaacgtggt tacagagatt tttgtgggca acaatgccca gctcgagttt gtgaagatgc
     5461 agaatgagca taacgatgca gctcagctta cccacaccta tgcacaccag caggccgatt
     5521 ccagactggt taccaacgtt ctttcgcttc acggtggttt tattcgcaat aatcttgctg
     5581 ttacacttgc aggtgagggt gccgatgcac agctctacgg cctttacctt actgaccgca
     5641 cccagcatac cgataacttt actttcattg accatgctgt tcccaattgc accagcaatg
     5701 agctctacaa gggaatactt gacgatcagg ctaccggtgc ctttaacggc aagatcatgg
     5761 ttaggcgcga tgcccaaaaa actcaggcct accagtcgaa caacaacctg ctgctcacag
     5821 ccgatgcccg catgaatacc aagccacagc ttgagatata cgccgatgat gttaaatgct
     5881 cccatggagc cacggttggt cagctcgaca acgaagccct tttctacatg cgggcacgcg
     5941 gaataggcga gcgcgaggca aaactgctgc tgatgttcgg ttttgcccat gaggtagtta
     6001 agcgaatttc cattgacacc ctcagggaac gcatcgacga tatggtcaac aaacgccttc
     6061 gcggtgaact atcgcgctgc cacaactgtc caatgcattg ctgttaggga gggactgaaa
     6121 ggggttgaag aggggaaggt tcgttgatag ttgttcgttg ttcgttgata gttgatggtt
     6181 gttcgaaata acaccttaat attatggact ataaggaact tgatgtgtgg aagtcttcaa
     6241 ttgaattggc aaaattagtt taccatttca ctaggcaatt accatcaaat gaagaatatg
     6301 gtctatccca tcaaatgaga cgcgcagctg tttcaattcc ttcaaatatt gccgagggta
     6361 ttgggcgaaa cacttcaaaa gaaacaatcc agttcctttt tattgcaaga ggttcaattt
     6421 ttgaacttga aactcaactt attttgtgca aggagatttt taaagttgat actcatgatt
     6481 atgacgctct cttccaaaag ctacaaaact gcaaaatgtt aataaacggc ttcattaatt
     6541 actatcaaaa aaagtaaagt gatgtttaac acgaacaacg aacaacgaac aacgaacaac
     6601 ctactaagct ttcgtgctga cttcccaatc ctatcccgcg aggtttacgg gcggcctttg
     6661 gtttactttg acaatggggc aacaacccag aaaccccttt gcgtgctgca aaccattgag
     6721 cgattctaca gggagataaa cgccaatatt caccgtgggg ttcataagct tagcgaggaa
     6781 tcgacacgtg agtatgagaa tgctcgcgag gttgttcgca aattccttaa tgcccgaaaa
     6841 acatccgaga taatatttac ctctgggact accgcatcaa tcaaccttgt ggcatactcg
     6901 tttggcgagg tgtttgtccg ccagggcgat gaggtgttgg taaccgaaat ggagcaccac
     6961 tccaacattg tgccctggca gctgctctgt gagcgtaagg gcgcaaccct aaaggtgctt
     7021 ccctttgata gcaatggcga gctaatgctc aataagctac ccgaattact taccccaaaa
     7081 acccgcatag ttgctgtaac gcaggtgtcg aacaccctgg gaacggttaa tcctatagcc
     7141 gagattgtgc gcatagcgca cagccaaaac atccccgtgc tggttgatgg cgctcagggg
     7201 gtaaagcatg gtaaaattga tgttcagcag ctcgatgccg acttttatgc cttctcgggg
     7261 cataagattt atggcccaac cggaataggt gtgctttacg gtaaggagga atggcttgag
     7321 cgtatggtgc cctggcaggg tggtggcgat atgattgcaa ccgtatcatt tgccaaaacc
     7381 accttcaacg agctgccgtt caagtttgag gcgggaacag ccaactatat cggtgcagcc
     7441 ggattggcta ccgcgcttaa ctactattca agcattggcc ccgatattgc caccgcatac
     7501 gaggatgagc tgctaaaata cgctactaca aagctaaaag agatagatgg gctaaccatt
     7561 tacggaaatg caccggaaaa ggcagccatc atctcgttct tgctcaacgg aattcatccg
     7621 tacgataccg gcatgatact cgataagatg ggcatagccg ttcgtacagg caaccactgc
     7681 acccaacccg ttattgacca cttcggcatc gatggcactg tgcgagcctc gctggcgttc
     7741 tataacacct ttgaggaagt tgaccgactg gtggaagccc tccataaggt aaaggagatg
     7801 tttggctagg ttccacagtg gtatgtgagc gtaagtttca gataaaggca tggaaaaaag
     7861 tccgcgaacc ttcttttggg gatttatatt aaggaaaaat attttctaac cgcacggttt
     7921 aaatttccgc gagatcgatg gatgttaggg tgttctaagg gaaaaattat caaattttcc
     7981 aattaaccag aacaaagtaa aaaccccgcc aaaccttaaa aactccaaca ttagttacta
     8041 taggtatatc aatgtcgttt agataacaaa acagtaactt cttttgtcat cctgagtgta
     8101 gcgaaggatc tccagtcaat gctaaaagtg aaaaagaaat aagagatgct tcggcgagct
     8161 tagcatgaca gagtgatgat tcgcttgcgc tcaacatggc attagtttca ctaacaacga
     8221 gtaacaatac acaaacaact ttatttgtca tcctgagcgc agcgaaggat ctctatttat
     8281 atatagtttt gagatgattc ggcagggcct atcccgatat gtcgggattc aacatgacaa
     8341 ataaccctaa ctgaacgacg ttgatttata atgagttaat ttgaaagaaa cttaataaag
     8401 aaagatgctt taaagtggag catctttctt tgtttttaat tatatttcaa tgtcgtttag
     8461 ttaaggaata ttcataatcg tttgtaattc atatagtaaa gtcgcttcgg ttttttattt
     8521 cgaggtactt ttctatttgg tcggattatt tcacgcgtgt tttgtacaat atcatcaaaa
     8581 gcttctaacg cagctttaaa ctgctttcgg ataaaaactc cgattaaaat atcctgggtc
     8641 attgaaatcg cattagtgcg gttgattttt tgatcgtgct ttctttcttt atctgcttta
     8701 tattcggcag taacccgttc ttctatggga tgagcatatg ccgcacataa agtcataagg
     8761 aatactttgg cataaaagtc ctgtttaact gctgtagctg ttttgccgga gaaggcttca
     8821 acttccacgc ggctttttag caacttgtag gcctcttctt ccgaccagcg ataatggtaa
     8881 agctcctcaa tgtctttatg ggtgtaatac tggtcgtcca gtaacgaggt gcacaatatt
     8941 tctatatcgc cattctcaag ctcaactttc actaatcggc aagttatagt ttgcccaagc
     9001 atttcagggt actccgatag ctttttaaca tcttttttgg gtagtgaaaa ggtcaccaaa
     9061 cgttcttttt cgtcactccg ggtaaagtct tttacctgta accaccaatc atcttttaac
     9121 ctaacacaaa actcaatttt ccttgccttt agcaggaaaa gcaaccaaaa acaaggatag
     9181 ccacggtcca ataagagtaa gtccccttct tctacatggc caaggtgttt catcaacaaa
     9241 tccctttcgc tcgatgcgta aggtgccaat tgggcatcaa tggttatttg gttcaacaca
     9301 tcatatagca tcgaccccat ggccagtgac cttggactat ctgctttggg gccaaactgg
     9361 tgttgcccga actcttcaat cacgctcgga tggtttggaa gtaccaacct tgtgccatca
     9421 acagccacta cccgtaaacc accccataca tcatagtttg ccttttcata aaacgcattg
     9481 acagcaacct catttaatcg ctgaaatgcc catgggttta gtttagccct tgcctgcgaa
     9541 aatgcacctt tggttacttc ccgtatttta aagtcgcttt tcgctatggc tttgaaaaat
     9601 gagtcaagct ccctttgtat ggaactttta aaattgatta ccaatatgat taagttattg
     9661 attgtgagtt tcctatcgcg tgtgaatgcc cctttgaggc catttttaga acggcctcta
     9721 aaactttcat tttcaagttc atacactatg tgttttgata aatactcacg gtaatcgaaa
     9781 ttgcttttaa tactagggat acccccttta tacccctaca aagtaagcgt actcatccta
     9841 aattacaaac ttttcaaaga actattttcc cctatatcct attctgtatg agctattcct
     9901 gtccttttgc ttaactaaac gacattgaat ttgcatttct gttattaaga agtttgaaaa
     9961 tagattttcc tagaagtgtt cctgttaaac ctccaagccc gactaaaatt ataaaatgct
    10021 catttgagtc aaacacagat ttcacttcat cgggcaaagc tatgtagtaa ttcgaccaat
    10081 catctatgct gtcatatttt ttgggaacgt tgcagtgaat agactttatt tttgaaggaa
    10141 actttggaag ttcttttgat gctatacatg tcaatttagc ttgcaacccc tgattgtagt
    10201 aatgctctaa tgtcagggaa cctcctcctc caagtcccac taaatgcaag atctaatgta
    10261 ttccttcctt acagaaatgt gattgatttg gttcttgttg gaaatcaaca gaaagcagat
    10321 ggaaatctgc tgcttccaat gttgaagtaa cattaatcac tcttcccttt ttcattttga
    10381 tttgatttat acaagtttaa cgctttatta tacacttctt taatgctatt aactaaactt
    10441 tctggtttga taaccttaac attctcccca aatgacagta attccataat aaaatcatgg
    10501 gtgatgaata tttttaactt tattctcagt tcattttcat tttcaaactc tatttgttga
    10561 gtttcatgaa gtggtaaaga tttaatataa ttcccctgaa agcgactaaa ggtcaaaata
    10621 acttcttgag gctctttcgc attcggccca attattccaa agcaatgcct ataatattca
    10681 ttcacatcaa aatcaatagg atatacgaat tgtttttttg tgattactaa ttgtgttaaa
    10741 cggtctagag caaagctttt aactttttta tcctttaaat ccctagcaag taaataccat
    10801 cgacttttaa attccttaag agcatagggt tctacaactc gttgggttga cgtattctcc
    10861 cagaattttt gataagaaaa tgatatttga accttatttt ttatagcatg tagcaaacta
    10921 tatagatttc cggtgccttg aggccgccgt ttttcaaagt gaatataatt tgaaagcctg
    10981 tcgctaatat ttaaagcgtt aaaagtatca aaagcctcta aaattctctc gtttacttct
    11041 ggttgctcgt caaagtcaat ataataaacc tttttcgaaa aatcatattg aatatcaata
    11101 ttataaatcg aacgaatatc ttcgatatcc ctttgaaatg tgcgttttga tacattgaaa
    11161 ttgtattcct gcaaatctga ttcaaaagcg aggtagtcag aaatttccga aaatgaagac
    11221 ggctgttttc tgagcttttt aattatcagg ttatatcgtg caattgattc tctcttcgac
    11281 atgattttta tgcttttgtg aaacctattc gaactcctct gttttttgat aataattccg
    11341 ttttgcagaa atcaagaata ctattaaaat ctactgtaat accgtgaatt atttcatgta
    11401 tctcaacctt tctaacaata ttatcaattt ggcctccaga gaaatcaaag cggtttgcaa
    11461 gtatttcaca ttctgattca gaaagacttg gtaattttga attccatatt ttggctttta
    11521 ccgaaatatc aggtttttga aattcaattt tgaataaaaa acgcctttca aatgccgaat
    11581 caagattttt tgcaaggttg gttgtggcaa aaaatatacc ttcaaaattt tcaagttctt
    11641 caagtataat gttttgaatt gcattttcgg tttgtgaaac attcgaggtg ctattctctt
    11701 tccgtttcgt aataattgca tcggcttcgt tgaacaataa aatgggcatt cgctcacagt
    11761 cttttgcata agccttgtaa tcagtgaaaa tacgttttat aattttctca ctttcaccaa
    11821 accacattga ttttgattga ctaatatcaa ctttcacaat ttccctatta gtgtccttag
    11881 caacttgaaa aacagtttct gtttttcctg tgccaggcga accatgcaat aaagcagtaa
    11941 tacctttcgg caaaccttta ctttgtaatc tattttgcgt tttcttgaaa ttatcttcct
    12001 gtaacaatgt ctttaataaa tcgagctgtt tacgctcaaa ttcattaaaa taaagagttt
    12061 tagagtttat ttttgctggt tcaataatat tatcacgttt ctttatatta gcgaaaagtt
    12121 ttaaccctaa ttcttgtagc atcttaaccg aagattcaga tagcttcatt tcggtataac
    12181 taaagaaatt agcttcgact atttctaaat gattttgact gattaactta ttttcattcg
    12241 acataatctt ttgaacatat ttaactcttt ctgaaggatt atcaaatata ctctctgtcg
    12301 cacgtccaat atcagtagtt tcattccctg ttatcgtttc ccaaatcaag taaagtaata
    12361 gatatgtatc ttcgatacta aactggtaat catttatttt cttgataaga gggaaatgta
    12421 aatttgaaga aataagctct ttagcttggg cgaatagctc aaaagtagat agttcttcgc
    12481 tatctcgttg ttcgcctaac ttatgaaatt tttcaagaag ttctatcaca tctttaaatc
    12541 cttcacgctc aattacaggc atcggtttat tgttcaagat tgcttctgta attttctcat
    12601 ttattgtaaa ctggtcgttt gaaagagcta atctaactct gtgtgaagat ttttgctttt
    12661 taaaaatacc ctttgaatat aacacatcga aatcatcact gaattccaat aatttcattg
    12721 gattacagtc aaaatactca atcaggtctt tcaaatcaac agtatcacct ttgtaattga
    12781 gggcgaaaac cattgcgaca attaatgact gatttctaga aaccttaaaa taatcggaaa
    12841 gatgttttaa ttcaacatca actttctcaa aaaatgctga atcaagcttg caatcttttg
    12901 attgctcata tactttgcct atactttcca ataatctaaa ttttatattc tgctccatat
    12961 ctgtaatatt ttgaacaaag atacaggcag agtgtgacaa tatatgtcgc agttgtttta
    13021 tactttaatt tctttgtaat tttcacctac aaccctcgtt ttctcaagta catatagtat
    13081 ggtttttttt tcgtttaaac atattctttt atgatagcag tctctcaaaa gagagtcatt
    13141 tttaattaaa tggcttcgat aatcatttaa aggtgtagcg ttctgaatca cattttttaa
    13201 aattgtgatt ctgtcatcac tcaaattaaa cgaattggaa attaacttat aaactgcatt
    13261 tataaactta tccgattttt ttctgaaagc atctttccat tcattttcag tattcaataa
    13321 tgagtatcta tgagcccaac caagagaaga tgtcctaccg tcccaaactg gatagtcgtc
    13381 ttgtgttagt aaggcttttc gtaataaatc atccttactt tcaaataaca ataaaaattt
    13441 gtcagaaata ctggtaaatt cttgaatctt atattcctcg ccaactttag aaaaatctaa
    13501 tagaaattta atttcacctt taaggaaaat gtcgtcttct gctttgaata tttgtgattt
    13561 ccaattatta tcagatttta tcaatgccgc ttttaatttt tcctcctcga tttgttttgt
    13621 cgcaaatccg gatttaagcg ttgtactgga aagaaattca taaatatttt cgcatccact
    13681 tgaaagttca ttgattaaac caattgcacc aataaaagca taggcactat caattgtagc
    13741 attttggacg atgttcctca ctactcttag ccaatttgaa aatgattcgg aatcaaagtt
    13801 ttctaaattt accaagtatt gtgtttgtgc atagaacaat actctttggg aataagtagt
    13861 ttcatttttt ttaataaatt caatgaacaa gttattatca atttctgtat cactgttgat
    13921 ttttactttt ttggttttac aaaaatccca taaagttaca ttggctggaa gtaattcatc
    13981 attctgattt tttgagtaaa catcaaagca tttttttaaa tattcaaaag attctttcgt
    14041 aggaaaatca taaggactaa catcggcagg attattaaaa agggctgtaa ttctgcgttc
    14101 tattctttct cttttaacag cttcgtcagt aatatttttt tctttctttt cttcgagaat
    14161 ttttcttgtc cttaattcat cttctgaatt ttccaagatt tcaagatttt gagcatagtt
    14221 atttattgct ataccagcaa taaattttat catctcgtta tcaataatat tatcttcacc
    14281 tctatgcttc caaaataagt ctgtccaaac tgtatcaatt ctatgtgaaa aggttttttc
    14341 tgtaagttcc ttccattttt ctttattatt ctcacttaat tcaagctcct tttcaaagac
    14401 attcttcttt atatgttttt caaatttggc tttaaagttc tcaaaatcag ttaatccttt
    14461 gcctctcgca ttcattttta tataaaggtc atcagttaag cctatatcat taagttgctt
    14521 gaaatgaaat aaaattggtg ggttttctga tgtcagtttt tgccaaaaaa tctttaactc
    14581 ctcatcattt ttgcttagta atcgttcttc aattgcatca agcattacta acattgcctt
    14641 tattgtaggg tcttttttcc acgacaaaaa gaaccatttt gaatcgtaaa ttttttcact
    14701 tattttttcc ccgatgccca aaaactctcc attatcaaca agctcattac aaaactctct
    14761 agatgatatt cttgtctcgt aagtaaactt tataaaatgc tttttcgtgt tcggcaattc
    14821 accagttttt agagcaatat accagtgaag caggaataaa gttgttaaac gttgttgccc
    14881 gtctaatggc tgaaaaacat ctttttcaac attcccataa acaaaatcca attcaagcga
    14941 acctttttca gattcaactg cttcaattat gctttctaag aataaagacc ttattttatt
    15001 ggttttctca ttttccctac cttgtgcaaa atcacgttga attatcggaa tctgaatctt
    15061 ggtcatttcg gtaagtaatt cccaaaacga tgaggaattt aaacgttcta ccaattcatt
    15121 ggctaaaacc ttaccgtcaa tatctttcat attatttgca attttttcga ttctttcaat
    15181 tttcatagaa cttggctttt aaaattgtaa acaactcttt ttaaatcatc aagataatca
    15241 gctcggtcgt tttcattcca tatttccaaa tccttcagtt cttttgaata atatttcata
    15301 aacacatttc ttgtacaaac aggaataaac gttccgtttt tttcattctc aattatttta
    15361 ttccttttat ttttgaaaga atcattttta taagaacggt ttgtacctct atcaagcaaa
    15421 cataggttgc gaatggtatt atcatcatca cctaaaacat attcgacaat tgactgaaaa
    15481 tcatcttctg caatttgatt tcctttaatt gcgttctcta tctgtgaatt aagctctaaa
    15541 ttagtatgtt gttctgtttt aacaaagttg taaaataacc acgtattttg gtcttcaaat
    15601 tttactttaa ccttagttgc aattgcatgg atatgttcaa tatcccattt ttctttttta
    15661 tatcgttcaa aaggaaaccg aatagtttca tttttattat tcaacattgt ttgtatatta
    15721 tgcagtaata aaatttttcg aatcaaatcc gaatgctttc catattccag ttcttccaaa
    15781 ttctcacatt taactttttc tgcaattttg atatcaagca gttttctaaa ttcttgtttt
    15841 tgtttgtttt tcacctcttt tagcaattct gttatatctg aatttgctga aattaaaaac
    15901 cctattttat gatatagttc tcggtctgta aaccattctt caatagtttg aaatatcctt
    15961 ttaatttctt tccagtttgt atcaatctca tctttgcttt ttgatttgaa tttttctgaa
    16021 aagaaacgaa atgttgaata ttggtcgttg ccgtttttta caccatcaat atttttcata
    16081 atgttgaaaa ggaactcaat acgagttggt agctcattat ctgaattatt tataaaatac
    16141 caaaactcat cattttgcaa tgagtattcc attctgtccc actcagatgc aatttctaat
    16201 tgtcttaatc ttatttcttc agtatcggaa tcgggaaagt ttgaactatt aagaaaaagg
    16261 gcttttatta attctgcgtt tgttaatggg attttcccca tattaatacg ggtaaagatt
    16321 tcaattgcat ctttttcgga cgaaacttca taccaaataa ctttcgtgct ttctagaaat
    16381 ggagtaacaa acttagaact tattgaatta taaccttgat ttgcctttgt ttgaaaccat
    16441 tctttaattt tttgatatgc ttgaaagata tgaaagtaat caatattttc tttagattcc
    16501 ttttcgctct ttaaatcaat gctttgtaga aatagtttgc tgttagagct ttctgtattc
    16561 cttgtttcat attctatttc aaaacttttc ttttcacttt ctatgaaacg ttccaagtgc
    16621 tttaaaatca aaaatattgt tgtcaatctt tgttgaccgt ctaaaacttc gtaatgatta
    16681 tttgtttttt tcacaaccac aggttgcaag caataccatt cgttatcctt ttttcctgtt
    16741 tttgtaataa aatcccatac atcatttaat aaatcttcaa cctgtctttc agtccatcga
    16801 taacctcttt gatatgaagg aataaagaat ttattttcta aaatttctcc gattgtcttt
    16861 tctgttaaac aactcatata tctatttatt ttgtaaatgg attaaaaaca attccgcaca
    16921 agctcttgca tcactcaaag catcatgatg gtctaaatga aatttataaa aattgcataa
    16981 agatgcaaga ttatctttaa aaatacggta tgtgcaatat atttcataat caggcgcttg
    17041 cattccgtaa tattcaagag ttttatttaa tactggaaaa tcaaaagaca aaccgttatg
    17101 ggcaacaacg gtttgctctt taataaatgg ttctatttta tgccaaactt tatcaaaagt
    17161 cggtgctttg gcggtatcat caggtgtaat tccatgaata tcaataaacc tatcccaata
    17221 gtaattgttg ggtggttgta ccaatatgtt tacttcattt gttattttcc catgctcaac
    17281 tctaacaagt ccaacttgac aaatactcca acggtaacct tgtgcagttt caaaatcaat
    17341 agcagtaaaa caatccatat tatttataat gctggtgcat gttttccttt tcctgctcca
    17401 tttggatgac gagaacaact actagctgtt aagctggaaa tacttgatga ttttgtgcca
    17461 caatacttac aaacatattg tgatttttct ccaccttcat aaagtttgtg ttttccttta
    17521 cctggtccat gggtgtcgtg gacatgaact tgctgttaag cttgaaacgc ttgatgattt
    17581 agttacgcaa tactcgcaat aaaaattagc cataagtttt ttagttttaa tttacgccta
    17641 ctctaaaagt tttcggcatt ccttatttag ggtcaaaatt aaatttcaag tcagccaatc
    17701 catgtcgtac tgttgcaatt ttcttcgtgc ggtggaaaaa acaactcttt tgcaaatttt
    17761 ggtcgtgagt ctgcttgtgc gatttgcaaa tgtgctgttt tgtgcgtcgg ctgtttccat
    17821 tgtcttgtct ttttgcagca aaattactgc tgtgtcgttt tttttcggct tgttgctaac
    17881 tcgtttatat gtatgataag aatcatacta attgctaaat taaagaatca tttcaaatta
    17941 tcaaaaggac attaattttc ccatactatt accaggtata tgtatgtatt tcagttgatt
    18001 tactaccatt gctcaagcaa tagtggtttg ccatactgtg cattcagtga aactttatcg
    18061 ggttaggctg ctcaacatgg caaatcacaa acaatttaat aaatagcata ttcccttact
    18121 agaaccttaa actatcgcta aacgcaaaca tactatctaa atttcattat cgctaaccaa
    18181 ttttcccgct aagacactat atttgtgagg taaatagtat agctgcctcc ataatgccct
    18241 ttgcccagaa aatagaggat gccattgaac ggttctactc ggccataacg gtgggggtta
    18301 accaccataa ggttgggata ctgagcacta ttgttgtgca cctgcttttg attgttgttt
    18361 ttttgtttct aaaaattgaa acccgcaagg agtactacgg ctcaaccatc gagatggagt
    18421 ttgaggagcc taaggaggag gctattgttc agcaaaagct tgagcctacg cttccgcccg
    18481 atgtgctaaa accggagtat gagactgagg ctatacgaaa ctttgctgtt gatgcctcaa
    18541 aaaccgattt gaacgctggc ctgagcgacg aaaagaatac cgatgccgat gagctttacc
    18601 gcgaggccaa tcaggtgtac gagcgcatgc agcagaaccg tgagctttac gagcagtcgc
    18661 aaaaggatat tgaggcaaac atacccaata cacccgaaaa gacagtccca aaatcgaagg
    18721 agggccagta taaggggcca accgtggttt cgtactacct cttaggccgt aaggccttgc
    18781 acctgcctgt cccttcgtat aaatgcgagc ttggcgggca ggttgtggtt aacattgagg
    18841 taaagcccga cggacgggta gccgatgcgg ttatcgacag ggccaactcg gtaaacgacg
    18901 actgcattaa ccaggcggct attcaggcag ccatggcaag tatttttact tccgtttcgg
    18961 gaactgcgcg ccagcggggt agtattacct acctttttgt tccccagtaa tgttcctaaa
    19021 aggcagccat aagcaggttg atatcgcgcg aggggatatc gtaaagcttg cccagatact
    19081 cgtttgtaag aataccgtta aacaggtaaa ctccatggcg taggccgcta tcctccttga
    19141 gctgcgaaag caaaccgcca ctctgtgcaa tttgaagcat taggggaaca aaaacgttac
    19201 tcatggcaat tgaggcggtt cgggccacac gggcagtgat gtttggcaca cagtagtgga
    19261 ttacgccatg cttaacatac gaggggttgc tatggtcgcg tatctccgag gtttcaaagc
    19321 atcccccacg gtcaattcct aggtcaataa ttacagcacc acgtttcatt aacctaacct
    19381 ggtcctccga taccaggtag cttaggttgg tttcatcggg ggctaaggtt ccaattacaa
    19441 catcggcggt tttcagaacc ttgtcaagca cctgcttgtg atatattgag gtgaaaaggc
    19501 gttgtcctag tatattttga atatccatca gttttttgat ggagttgtcg aatattttta
    19561 cagtggctcc tagtccaagc gctgcacgtg cagcatactc cccggcagtg cctgctccca
    19621 gaataaccac ctcagtgggc gatattccgg ttagcccacc tagcataaca cctttccctt
    19681 tattcacgtt gctaaggtac tcggcagcaa ccagcaccga tgtactcccg gcaatggagc
    19741 tcatggcccg aacaaagggg aattcgccgt cagcatcgga tatgctctca aaggcaatgg
    19801 ccgtaacacg tttctggata aggcttttga ggtagtcgcc ggtgtggttg ttcaggtgta
    19861 gcgatgagag taccgattgg ttttcgcgca ggtattcata ctccttaggg gtaaggggtg
    19921 ctaccttgag tacaatatcg ctttggtaaa cctccgatgc cgtggctact atgcgtgcac
    19981 ctttctcgct gtactccatg tcggagtagt tagcctgcct acccgcatcc ttttcaatga
    20041 tgatttgatg cccgttctgt acaagtatct caacggcttc gggcgttaac ggaattcgac
    20101 tctcggtggg gtcaacctct ttgggtattc caatggtaag ctgtttgtac ttcttcttat
    20161 attcaatgcg ttcctcctga actagcaatc cctttgcatg gggaaactcg gaaaatttag
    20221 gactgttcat gcccgttagt ttttaaaaat gcaagttagc acatattaac ttatgcacaa
    20281 acctgttagg gtataaaagc aatatgtatg atttttacga tttacaaagg tttaaggtta
    20341 tagctaatcc gatatttgta atgtgcggct tccgtcggca ttcacagcaa tttggatgtt
    20401 tacaacccct tgggttggga gaatctgctc aatgagttcc ggccattcaa tcagacatag
    20461 ggcttcgggg ttgtagaagt actcctcgta accaaaatcg taaacctcat caatacgttt
    20521 aatgcggtaa aagtcgaaat ggtaaacggg cttgccgttt cggtcggtat actcattaac
    20581 cagcgcaaac gatgggcttg taaccacatc ggcaacgcca agcgccttac agattgccct
    20641 tataagggta gttttgccgg ctcccattgt gccgtaaaag caaactatgt ttttaccatg
    20701 aatagcgttt aatacccttt gggctgcctc gtcaatctgc tgtatgcttt taatttcaat
    20761 ggtggtcatg ttacctcttt atggggttaa gggttacaag cggaactatc atctcctcaa
    20821 gcgatatgcc tccatgctgg aatgtgtcgc ggtagtacga tgcgtagtag ttaaagttat
    20881 tggggtaggc caaaaaatcg tttcccagag caaagataaa cgaggagcta atgttgggtt
    20941 tgggcagatg cgcctcctcg ggcttgcgaa tctcaaaaac ttctttgggg ttgtagttta
    21001 agcttcgtcc cagcttatag cggaggttgg ttgatgtggc tctatcgcca actaccttca
    21061 ccggattttt tacgcgaatt gctccatggt cagttgtgag caccacctgt actccctggc
    21121 ttgaggccag gtccaaaaac tcctttaggt cggagtgcag gaaccacgag cgggttagcg
    21181 ataggtaagc cgattcgtcg gcggccaacc cgcgcatcat ctcggaatcg gttcgggagt
    21241 gcgacattac gtccacaaag ttgtaaacca gcaccgcaag gttgcttcct aaaaactcct
    21301 tgatgtttag gcctccggca atacccttca gggtatcggt tttaatgtac gtgaagtttg
    21361 tgtttagtcc aagccgctga agctgtgcta ttagtagctg tttctcaaaa aggtttttac
    21421 cttcatcctc ctcgtcgaaa acccataaat cggggtaggt tttatggatt tcgagtggca
    21481 tcaaacctga aaaaagcgca ttgcgggcgt actgcgtggc agtagggagt atgctgaaat
    21541 aggtatcctc actgctaacc tgccagcttt cggcaattat gggttcaatg gttttccatt
    21601 ggtcgtagcg gaggttatca attaccagta gtaaaacctt gctaccggaa ctgataatgg
    21661 ggaaaacttt gcttttcaga accgatggcg agagcaaggg tttgtctcct tgagtttgga
    21721 accattgcaa gtagtttgcc ttaacaaatt taccaaaaac ctgattggct tcctcgaact
    21781 gcatctcgag catttgcttg aggtttgagt ccggattacc cgatagcttt acctgccaca
    21841 tggcaagctt tcggtatatc tctgtccagt ccaggtgggt tttagcggcc gaaattaagt
    21901 ccgatatcaa cctaaactca tttcggtagt cggcaagctg cttttcggtt accagctcct
    21961 tgccatgaat aaactttttt acaaccagca gtacctgctt ggggttaacg ggtttaataa
    22021 ggtagtcgga tatttgagca cccaccgcca tgtccatgat attctcctcc tcgcttttgg
    22081 taaccataac cacaggcagc gccgatttta tcgccttaat ttttgggatg gtttcaagcc
    22141 ctccaatacc gggcatattc tcgtccagga acaccaaatc gatagggttc tggcttacca
    22201 gttcaagtgc atcgtgtccg ttgctggcgg taagaacatg gtagcccttt tgctccagaa
    22261 aaatgatatg cggacggagc aaatcaatct catcatctac ccatagaatg gtgggctgca
    22321 ttgttctact ctgttttata gttagcctta aagaagttca ggtagtcggg tatcgattta
    22381 tcgctcaaaa agcgtgcaaa attatcgata cttatcacaa acatttggta atcggtgggt
    22441 ttaaggcctg caaacacttt tggctccttg cttatcatct taaagtactt tatggccgtt
    22501 tgtttatcct tcagacccgt acacgtaatc atctcaatgt aatcggtaaa gggttgattg
    22561 gctacattga ggtcggcatc aaggaattca tcaacattga atgagatgat gttgaacttg
    22621 gtttggttga tgttggagcg tttgggaacc agcatcacaa acatgtgctc gccatcggta
    22681 gctgaataaa ttgatgcttg tatttcggtt tgcggggtta ccggagcaat atccaaatcg
    22741 gtttgcccgg aaatgagctg cagctcgcgc tgctcaacct gtttcagcat cacttcagct
    22801 aactgaccct cctctgtttt ggggaagttt gcagccagat tggaaagaga ggttttgaac
    22861 accttaagtt ccgcagtttt tgcctggcaa agagcttcaa gcagcataaa ctttggctca
    22921 aggggagtgt tcttagtttt tgctaagccc ccttgtacga tgcttagggc ctcggagagt
    22981 tggcccttgg agtaaagctc atacgctttt tggtaaaggg cttcgccctc attctgttgc
    23041 ttgctcagct gctcaaagta ggccgggttt gagaggagta ttgcataagg gcttttgggg
    23101 taatttgtaa taagcgtact ccggaaaagg gctgcatcgt cctgccggtt atactgcgat
    23161 gccatacggt atagcctgta gtatgcattt gcagcaatat cctgttcagc aaatcgttgc
    23221 gcaagtttta ggtatgctgc tttagcctcg ttgtaatcgg aaaggttaac ctcgtaaatt
    23281 tcagcaaccc tgagcatggc gtttttaatt ttctcaacgg agagctgttt cagcgagtcg
    23341 ctgaaaggta ggttaaccag gtagtactcg ggtgttttag gcgatagctg cttttggggc
    23401 tcttgtcctg aggcgttgcc ggttccagcc actgtaacct cgtcgctgct gagcacccgt
    23461 ttgttttttc ggcgccagtt gtcctcaagc ttacgcttgc cccacttcat ctgaaactcc
    23521 gattgtccat agcttagcga tgcctggttg tagaaatacc atttgccacc ctcctgggtg
    23581 ttctcggttc ggtatcgctg ggtttgctgg taaagcaggg attggttcct ttcttcctgc
    23641 tccattgcgc gagctcgctg ttcctcaact tcaatgttgg ctataatggc tttgataagg
    23701 gcatcgcgct cctccttggg catagctgca acccttaata ggctatcctc ggttttaatg
    23761 gtggtaatgt tttcaactag tgagttgagg tattttgctt tctgctcaat tcgttcaatc
    23821 tcgggatagg tattatccaa cgagttgtat gagctatcgt agtaggcctg ggcacgggtg
    23881 tagtcgttct ttgaaaagta gtagtcggcc aacaccaggt acgacatccc cttttggttg
    23941 ctattgcttt tgctatactt tgctgatagg gtatagtact caatagcttt atcgatattg
    24001 ccatcttttt tctccagatt cccaagagtg aaatagagct ggtcaaggta atcggcgttt
    24061 ttagcgtcct tggaaagctt gataagctca cgcttgagct cgtcgccctg tttttggcta
    24121 agtgcaatca tttggatgcg tgcgctaaag gccatatcgt aggcggggtt caacctcaga
    24181 actttttggt atgcaaccag ggcttcgccg tttttcccaa cctgctgtag ggtttgggca
    24241 agaatgtagg tgtacctaat tttatcgttc cttttggggg cgagctcaat agctttccta
    24301 aggtgtggga gcattgcatc gggttgctgc tgcttttggt agtagtaagc ccatgttgat
    24361 gaaagaagat ggttaaactc cttgttacga gggcgcttac ggttgctttc aaccgatttt
    24421 agcctatcct gcgcatcaac aaaatcgccc tccagaattg caactcgtgc caacaaaacc
    24481 tgtgcctcaa accatatggg taatcccgga aactgcctga ttacaaactc aaatgttgag
    24541 cgggctttag tcaagtcctg tttccatata tgagctttac caaccagcag ccacgattcc
    24601 cttgcccatt taacatactc gttaaggctc atgaattcct tttctttagg gcttagggtg
    24661 ttcttggtac gtgccggctt tacgtttatt gagtgccgcg caagcagcat ggtacacttg
    24721 ttgagagtgc gctccatatc gcttgataca attccgggca cttggctttc gcctatcagc
    24781 aaaacaggta atggcagggt gtaattgtag tttatcccct tttcggcttt ataggtaccg
    24841 cttttgtagc tatcgtaggc gttgtagtag taattgtaat atgaggttag attatggtag
    24901 tttcggctca caaacgtatt cttttgtgtt gagcacgacc atacccctaa tagtaaaaca
    24961 atgcccagaa gtagcagcaa tcgggtgtaa cttgatttta tcaactcaaa acaattttgc
    25021 attacaaaaa cggataatag ccccaaaaag tatacagggg cacaatatta caaataattg
    25081 tggactttgg gtttaattcc tttaccttct tcaacggaaa aacgctactc tacctcaatt
    25141 accaaatact ttgaaaccga ccagaatttt tgggtatcgg taattttaag ctcttcaatt
    25201 attcccttct cgttggttgt aagggtatac gactccttgg gatgggttgt aacaacagtc
    25261 gcttttttag ctgatagagg gatgctcacg gttttagtta tatcgatacg tacaaagtag
    25321 ttctggttaa attcagcttt cattttgctc gatttgccaa tccctaggaa acctccggtt
    25381 ttgtcgataa ccttattatc aattaattcc cgccttgttc caatggcata ccaggctgtg
    25441 ttgagctgtt cggtttggct ttgaattttt acactcatct caaccttttc ctccattagc
    25501 gtatcgatgc gcgcatttag ctgggttacc gtaaagttaa gttgggtaag attttcctta
    25561 agctgttcaa tttcacgttc cttctcctct agctgttggg taacattggc aatcattcgt
    25621 tcaaactcag ccaccttaag gttagcttct ttcagctttt ttcggagtgc cgcaatggtt
    25681 tggcggttct tatccatcag catgttgatg gtttggatat cctcatcaat ttgttggcgc
    25741 acatcgggct ttagctcact accagcaata gcattcttgc taatggtttg ctccttctgc
    25801 ttaataacgc tcaggttttg ctcaatctca cccataaagg cgaaaaactg attaatggta
    25861 gaatctttaa ttcttgcctc gttgcgtaag gcttcgtttt ccttggttag tagttcaatc
    25921 ttttttttgc ttgaaccatc gcacgatacc agcaaatagg caaacgtaat tacaatagct
    25981 actcttgcta taccctgtgc tttcatatgt gtaaaataat tggtttgaag tgtgcaattt
    26041 aggtaaaact ttgtttagtt gagttttagg gagcaggaat tttgggaata aactgtatag
    26101 cataaaattt ttatgaatca ctactgttct gctaaagttt tttggtattt gtcatgctga
    26161 gcatcgccga agcatctctt ggaacattca acccttttga ttgtattacg gcaatctctc
    26221 tttgccatgc atttctgttg cgatgcaact tttcagtgtt gttctttgga aaaagtattg
    26281 cttgcatcgc aatacaagtg ggataaacca tggagttaca aatgtttagc ccctacgggg
    26341 cagtaagcgt tgttcgttat tcgtgaatcg ttgttcgtga atatcacgtt acacgctctg
    26401 tgtcacaccg tgttacactg tgtaactctg tgatacttgc ttttgctcgg ttcttgagac
    26461 agttgtcatg ctgaactcgc cgaagcatct ctttgtacct taaactctat taattagatt
    26521 aatctagcaa caaaggttgg cataggaaag ggcataaaag cctgacccgg caaagggtcg
    26581 ggggtggaac atggcggtgg gcttactaag cgataccaca tgagtcacgc cgtaggcgtg
    26641 gcgaatagta tcgcgtggca gttccaccca cggggcaccc atcgggtcag gcttttcagc
    26701 cttgattttc tttggttctt tcttgtatca agacaagaaa gaacatctaa cccactcttt
    26761 gccatgcatt tctgttgcga tgcaacgttt cagtggtgct ctttggaaaa ggtattgcgt
    26821 gcatcgcaat acaagtggga taaaccatgg agttacaaat gtttagcccc tacggggcag
    26881 taagcgttgt tcgttgttcg tgaatcgttg ttcgtgaatg ccattcagtg taacaccgtg
    26941 ttacactgtg taactctgag atacctattt agttgatggt tgatagttga tggttgatag
    27001 ttgatgataa atcctaaacc tatgacattg acccttttac atgaaccttg aaccttgaac
    27061 ctttatcctt tatccttaac cccactgtac actgcatact gtacactgta cactaagaat
    27121 tatatccttc actttgcaca acatgacaaa aggaggggct gttttgtatt ctaaacgaca
    27181 tttcgataaa gaactttgat tggacagtat ataatgcttc attaataaaa aagctatttt
    27241 tccctacatt ttttgcagga atccttctac tttatatctt tacgtgtcaa aatacacagt
    27301 atttacaaaa atcattgaaa agcgatgcat ttacttgaag ctaaaggaat agagaaacgc
    27361 tactccaacc atttggcgct cgacgatgtt agcatatcgg tgccaagggg tagtatctac
    27421 ggccttttgg ggccaaatgg agcgggtaaa actaccctta ttcgtatcat aaaccaaatt
    27481 actggccccg ataggggcga ggtgctattt gatggtcggc acatgcaacc taacgatatt
    27541 taccgtattg gttacctacc cgaggaaagg ggactttaca agaagatgaa ggtgggtgaa
    27601 caggccttat tccttgcaca gctcaagggt cttagccgtg ctgaggccat gaagcggtta
    27661 aaatactggt ttgagaaatt cgaaattcag gcctggtggg ataaaaaaat tgaggagctc
    27721 tcaaagggta tggctcaaaa ggttcagttt atggttaccg ttatccatga gcctgagctg
    27781 ctcatttttg atgagccgtt tagcgggttc gaccccataa acgttcagct cgtaaaggac
    27841 gaaatactgg aactgaaacg caaaggaaca accattatct tctcaaccca caatatggca
    27901 tcggttgagg agctttgcga ccatatcacc ctgatcaaca agtcgcgtaa catcctaagc
    27961 gggcaaattg atgaggtgcg ccagagttac ggtagtaatg ttttcgaaat agctttccgt
    28021 ggaaatcccg attcactaag gcaaaaggtt gaaggtaggt tcgaactgtt gaagattgat
    28081 accgacaact cactacctaa ggcgcaggtt agattaaaat cgcaatccga tgccaatagc
    28141 cttttgcagg aattgattcc agctgttgaa atagtttcct tcaatcccat tgtgcctagc
    28201 atgcacgata tttttataca ggtagtgaag gagtataacc aaaagcacgg tttagtaatc
    28261 agttaggtta aaacttaaac tagaaaatag tatgaacaag atatttctca taatgcaacg
    28321 cgagttcatg accagggtca ggaaaaaatc gtttatcata accaccctgc tctcgccaat
    28381 attttttgca gccatagcca tacttccaag ctacctagct tcgcttgagg ttaccgaggt
    28441 acgaaccgtt gccgtggtcg ataataccaa acagtttatt gatgttattc cctcaactga
    28501 gtatatcaag gttacttacg taaccgatac tccagccgag aaggttaagg aaaacttaaa
    28561 tgaaacaggc tattatgccc tattggtgat tgactctact tccacaccta atcaggcagc
    28621 ttgcaccatt tactcccaga aacagcctgc cattgatgtt atgcagcacc tggagaatac
    28681 tttagaaaag gaaattgagg cgcgcaagct taaaacctat aatatagata atcttgataa
    28741 gatacttgcc gatgttaaaa ccagtatctc aataaaatcg ataaaggtaa gccaaacagg
    28801 ggaggaaaag gagagtaaca ccctaattgc tatggcaata gcttacatca tgagcttttt
    28861 gatgtatatg atggtactta taaccggtaa ccaggttatg cagggggtta ttgaggagaa
    28921 aacaaaccgc gtggttgagg ttatcatttc atcggttaag ccgtttcaga tgatggcagg
    28981 taaaattttt gggatggcct cagtgagcct gctgcaaata ctgatatggg tggtaatgac
    29041 cgctgcgctt gccggggttg gtatcaacat cataagcagt aaaatgacca caaaacccgc
    29101 taccgagcaa atgcaggcca tggcccaggc tgatccccaa atggcacagg ttcaggaaca
    29161 ggctacagca gtagcaaaca gtgagggaat gagtttcctt acggccctca agaaccagaa
    29221 cttcccatta ctcattggag gatttatatt ctacttcctt tttgggtact tactttatgc
    29281 ggctatgtat gcagccgtag gctcagcagt ggaaagtata accgataccc aacagctcac
    29341 actgccaatt accctaccgc tgatactggc aataattgtg atgattagcg gaattaagtc
    29401 gcccgatggg cctttagctt tctggttctc gctaattcct tttacctcgc cggttattat
    29461 gctaacccgt ttgccttttg gcgttccaac ttggcagttg gcactatcgg ctactctgct
    29521 tgtgggtaca tttatcctta ttacctggct agccgccaaa atatacaggg tgggcatact
    29581 aatgtacggt aaaaagtaca cctggaaaga gatgattaag tggattagct acaggggata
    29641 gagtttacaa tgcttaccat tgagcagcta attgaaagtt atgataaacc cggcacggtc
    29701 atcctccttg agggtaaacg tgccgtttta cctaccgacc agcacaagct aattgaggtg
    29761 ggtaagttgc tcgcaggcag cagtaaacat attgttttca ggagcggaaa tgccaaaggc
    29821 gccgattact tcttttcgca gggagtttcg atggttaacc catcgcgctt acagcaagta
    29881 accccgtacc gcggccatag aacgaaagag actttaggca ggcaaacggt ttcgctcgac
    29941 gatgttgacc tggcaagtga acctaacgta atatttcaca ctaagaacag cagcaatgta
    30001 tcgcagctta tcgatagttt tctttcactt ggcgataatg ctctaaccgt taaggcccga
    30061 tatttactcc gcgataccat aaaggtggtg ggtacctcta agttgcagcc tgcaacttta
    30121 gccatttttt acgatgattt atcgaaaccc aaaacgggtg gcactgggca caccatgcgt
    30181 gtgtgtgaaa aaaatggaat cccctatttc gaccaacggg tgtggtttaa gtgggtggaa
    30241 cagtaaaccc tttagggctt gggtttttcc atcttcatcg agatggtttt attgaaaatt
    30301 cctgcgctaa caagtttgtc aaactctcgc gaattactcg gttttaggat aatcttggag
    30361 ttctctttgt tttcgtcttt aattttctcg aatggcacta tgttgcctat ttccttttca
    30421 acctcatcaa gcttgtttgg gtcgaaatcg gaaaagctaa tataaagtga atcgttgctt
    30481 agcttaaaaa tgtaagcaat ccataccaat cgacccttaa cgctatcgga aaagttaaca
    30541 acaaacccac tggagtattc cctaacatca aggttctttc ctaaggttcc ggttacagta
    30601 aatttatttc cactaccaag cgatatggta ttcctggtga tagttagtgt gtcgttatcg
    30661 gaactgataa aaattcctgg aaatgcattg tttaggttag tgatgatttt cccgtcaact
    30721 ggctgaggct tgttaaatac aatgttattg cacgaggtaa aaaaaagtgc aaataacagt
    30781 atgaattgag tatttctttt cattaaactt tactttttcc ctctaaatag gtatacaaaa
    30841 cctttagcgc tttcatattg cccggaaagg tcttcgataa cgtagtagta aaccccttcg
    30901 ggcatatcgg taccttggtt gttcttgcca tcccaaacca aaacactcga ttccgccttg
    30961 taaattaacc cacctgaacg ggaaaatata cgaaaatcga accatgccgt gccgctggtg
    31021 ggtacaataa agttatcgtt taccccgtca gagttagggg taaatacgtt agggatattt
    31081 tcaagcggaa cctttccgct agtaaagtcg ggcaggactc ttattgtttg agtaaactcg
    31141 gctgtttgtc cgctgtttga attacgcaca tcaagcttta cctgatgtaa tcctgcagtg
    31201 gtaaaggtat agtaaatgtt tggccactgg tcgagcgcta catcgtcgaa agcgtttccg
    31261 gccaaagggc taccatcaac cgtccaggtg aagcggaggt ttcccaagct atcggggtgg
    31321 ttgggtattc ggtaagcatt aaggaaaatg cgtttgtacg aggcaagtcg gcctaaattt
    31381 ctatcgggga aaacaaaaaa gaaagcaggg ctaacaggaa tctcgcgtga gtgcgatagg
    31441 ctttcgccat tcagggtata gttcagggta acggttacac tatcgtccca gcttgtatag
    31501 gagtatgtgt gtgtgggcgc aacctcggtc gatgtgttgg agtccccaaa atcccatcca
    31561 gttggtgtta cgcctgctct gggtataaac tttagggttg attggcttcc gatgttgttt
    31621 gtttcaacta gttgccatgt gaaaaccgaa tcggcttcaa aattttgtga aaagcttagg
    31681 catggtaaaa gcattgcaat tgcaatggta gctgcagttg aggtaatatc tttaatcatt
    31741 gccggcatgg tttatcaggt aaaattaagc aaaatcgata ttctgtctat atcttttcgg
    31801 ttaatttttg aacaacccta aagttattaa aaaattcctt agcaaaaaca cgaattactg
    31861 tatagcttgg tatggcaagc aacattccaa gtattccggc aacactacct gcaaccagga
    31921 gaacaataaa aatttcaagg ggatgagctt ttacactgtt tccgtaaatt aatggttgaa
    31981 aaacaacatt gtcgataagc tgggtaatta ggaataccga aaccaccagg attgtaaatg
    32041 taagcatgtt ggctgtaaac cctaggtgaa gctgtgtagc caatactatg agcgtaccaa
    32101 gcactgttcc tgtaagtggc cctacataag gcactacatt cattatacct gcaattaagc
    32161 caaccaccac tgcagtttga aaacgaacgc caataatggt aagcccaatg gtgtcgagca
    32221 ccattatggc agtgctctca attactatac ccacaaagta gcggcgcagg agcttgttta
    32281 tactaaccag cgctcgagtt atttgcattt cgtatttttc agggaatagc agaattacgc
    32341 ttttaaaaaa gagcgtgtcg tccttaagga aaaagaaagt tatgaagctt atggaaaaca
    32401 aggcaataac caggctaatt agcaggttag ccgtggatga aaaggcatta gttatcattg
    32461 aaaccgtaaa aatccccgat atttttgtta tcaaaaaatc ctgcagcaca aatccttggg
    32521 cgcttgccgg taagtagttt tgaatgaatt gttgaatgga attaattggt tcggcaaacg
    32581 atgccactag gctttgtaca tcaattgacc ccaactcgtt gaattgtgca attacaagtg
    32641 gaatcattac cctgaagaat gctatgaaaa gtatccagat tgtaatgagg gcaatggtgg
    32701 ctcccaaaaa acggggtggt gaccagtttc tgattctgat tgaagaaata aaatcgacaa
    32761 taggcttacc cattaggcta agcactgccg atatgagtat gtaggctact attgatgaaa
    32821 agtaccaaac caaaaaagca accagtgcta ctacaaatcc aataatgata taccgcacaa
    32881 gctggttcat actcctaaaa tttatcaaac ctaatacaaa atgctgaaaa atcaaaatgg
    32941 ggggtgatgg ctaaaggtaa atagttaatg ggggtttgta agccgtgaat cgttaaacga
    33001 atatggggaa gttaatggag gttagaaccc gttaaacagg gctatactgg cccttggcct
    33061 cggtctcctg atttctgatt cctgacttct tcatgaaaaa tttaaaaatc attacttttg
    33121 caaatcatga accaatcaaa ttacatacat cactttttcc gatgttgcct ttagtttagg
    33181 gcaattaaat aattctatgc aatttttgtc ttattaaatc ttactagttt gatatgaacc
    33241 aaatagctat ttacaataca cttacccgca aaaaagaagt ttttgagccg cttaacccac
    33301 ctcatgtagg tttgtatgtt tgcggaccta ccgtttatgg cgatccgcat ctgggccatg
    33361 cccgtccggc aatcaccttc gatttgcttt tccgttacct caagcatttg gggtataagg
    33421 ttcgttatgt aaggaatatt accgatgttg ggcatttgga gaacgatgcc gatgagggtg
    33481 aagacaagat agccaaaaaa gctaggcttg agcagcttga gcccatggag gtggttcagt
    33541 actataccga taggtaccac agcgccatga agttgctgaa cgtattgcca ccaagtattg
    33601 aacctagggc atcggggcat ataattgagc agattgagtt ggttaaaaaa atcattgata
    33661 acggttttgc atacgagcgg aatggttcta tttactttga tgtggttaaa tacaacaaaa
    33721 agcataacta tggcattctc tccggccgta atattgacga tttaatggcc aatacccgtc
    33781 agctcgacgg gcaggatgag aagcaaaatc cgtttgattt tgcgctttgg aaaaaagcat
    33841 caccacagca tattatgcgt tggccatcgc cgtggagcga aggctttccc ggttggcatt
    33901 tggagtgctc ggcaatgagt accaagtacc ttggtgaacc tttcgatatt catgggggtg
    33961 gcatggattt gcttttcccg caccatgagt gcgagattgc ccagagcgta gcggccaacg
    34021 gcaagcaagc tgtgaggtat tggatgcaca acaacatgat taccattaac ggtcagaaaa
    34081 tgggtaagtc ccttgggaat tttatcaccc ttgaggagct ttttacgggt acacacaggt
    34141 tgcttgagca ggcctatagc ccaatgacca taaggttttt tatgcttcag gctcagtacc
    34201 gttccaccct cgatttctcg aacgaagcgc tacaggctgc cgacaagggc cttgcccgcc
    34261 tgatggcggc aaaccgccag ctcgatcgca ttaaaccatc ggcaacctca agtgtaaggg
    34321 tcgatttgct aaaggaaaag gtttacgaag ctttaaacga cgacctcaac agcccagttg
    34381 ccattgcggt tcttttcgat tgggttaggc aaattaacct gcttgccgag ggcaaggaaa
    34441 ccattaacga ttccgattta catgagctaa aatcgttctt tagaacagtt atctttgata
    34501 tccttggact tgttgatgag catacgtcgg gctcaaagca tgctgagcta acagcaaagc
    34561 tggttgagat gctgctgagt atgcgcatgg aggcaaaaca gcgaaaggac tttgccacat
    34621 ccgaccggat tcgcgatgag cttgcaaagt taggggtaat tgttcgcgac cgtaaggatg
    34681 ggttcgactg ggagctggag tagagtattg aaaaagaatc tgtaaagagt ttaaaaataa
    34741 attacttaaa atggatatta ggtggaagca acgattcgac aattttgaaa aagcgttttt
    34801 acgtttaaag gacgctttaa gaaatgactc tccatccgaa ctggagaaag ccggagtgat
    34861 acaatacttt gagtttactt ttgaacttgg atggaagaca ctgaaggatt accttcagga
    34921 gatgggtgtt gaagcaagat ttccacgcga taccataaag gaagcattca ctaacggact
    34981 tattcaagat ggagacctat ggctcgacat gcttgaaaaa cggaaccaaa tgtcgcatac
    35041 ctacgatgaa tttactgcaa atcaatcgtt ccaaatggtg cgcgatgaat ttattcatgg
    35101 tattgagcag ctctacacta aattaaagga tcttaggtaa tggaaacatt tggtatatcc
    35161 gagaagagtt atttactgat cattgatgtt ttaaaaagtt atcctgaggt agaaactgca
    35221 attatttttg ggagtagagc aaaaggaaat tataaaaacg ggagcgatat tgaccttgcc
    35281 ttgattggtg agagtttaaa cagcaaaata attttagaca ttgctggtat tctgaatgag
    35341 ggaattccta ttccctacca tgttgatgtg gttaactacc attcaatctc tactcctgaa
    35401 cttaaggagc atatcgatag ggtaggcaag gaattttaca aaaaggggtg ttgaccaagc
    35461 aacacccttt tttattaaaa ctctcggaaa ccaataatgt gccttacatc gcgtatggtt
    35521 ttcgatgcgc tttcgcgagc tttttcagcc cccattaggg ctacttttct taggtaatcg
    35581 ttatcggcaa gtatagcctc aatccgctcg cggattggtg ttgtaaaagt aattatatcc
    35641 tcggccagct gctttttcat atcgccgtag cgaatctcac aggcattata cttatcctca
    35701 aagtactgga gcgtatcggg cgatgaaaca gctttcatga tagtgaaaag gttctggatg
    35761 gcctccggtt taggttgatt tggttgggtt gggcctgcat cggtaaccgc tttcatcact
    35821 ttctttcgga ttgtctcggg atcatcggaa aggtaaatgg cattcccttc ggacttaccc
    35881 attttacctg agccatcgag gccgggaatt ttaactaaat cctcaccaaa gttaaaggca
    35941 accggttcag ggaagtaatc taccttatac attctattga atcggttggc aaaggtgcga
    36001 gtcatttcca gatgctgttc ttggtccttg cccacaggca ccttggttgc cttgtgaata
    36061 agaatatcgg cagccattag ggtgggatag gtgagtagtc ctgcattaat gttgtcgggt
    36121 tgcttgcgaa ccttttcctt aaacgatgtg catcgctcaa gctcgccctt gtatgcgttc
    36181 atgttaagca agaggtaaag ctcaggaatc tggggtacat cgctttgtat gtaaagtgta
    36241 gccacttcag ggtcaaggcc gcaggctaag tattcggcaa gcacctgttt tacagaacca
    36301 tgtaaatcct cgggggtagg atgtgtggta agcgaatggt aatcggcaat aaaaaagaag
    36361 catttattct catgctgcat gcgaacaaag ttgcgcagcg ctccaaagta attgccaagg
    36421 tgaagttttc cggttgagcg tattccgcta agtacaatat ccattttcct aaatttttgg
    36481 caaaaataac attttttagg gtttattaag cgttggtttt tgtttcagga actcgttcag
    36541 ggggatgggt ttgttttgtt ttgcccattg gtaggttagc tcatactgtt ctttgaggtt
    36601 gttaaaatcg aacatcggga agctatacct atagctggga acaaaaacaa gggttttggc
    36661 agcaagttcg gggtagcgtt tctttatgtg ctcaagcctt cgccagaaac ctagcgatgt
    36721 tagcacatcg ggcgataaac cattatttaa tttagttttt agctcatacc caaaaggttt
    36781 tagctcatcg gcaagggtca tgtccgtatc gcgcttgcgc gaaataatga ttacacgttc
    36841 aacgggttgt ttcctgtaat tctcaaactc aatgagtgcc tgaaagggaa cacggtcgtc
    36901 gccaatgcca ccatcatagt aagggatatc gggaatggct tttatgccgt taattcggga
    36961 tgggggaaaa acaagcgggt aggccgttga tgccattaat acgtccacta ggtttagggt
    37021 tgaatcgctt tcacgattga ttttacggtt gcagaaacgg tatgtccctt cactcaatgg
    37081 cgaaggccgt aagctaacca ctgatatggc agtaggaaaa ggcaaatcgg ccaaggtctt
    37141 atatcctagc cggttaacca caatttcttc cagcagtttt cgtaaaggct cgttgctaac
    37201 cggcaagccc tttttatcgg ttctgaaaac attatcgttt ttaatatcgc caaaaagctc
    37261 gcgatactct ttccatgtaa gttttttctc gagcactgcg tttagcataa ctgcattaag
    37321 cgcacctgaa ctcgcccctg aaatgaaaac aacatcgtta agttcgccac gctcgtaaag
    37381 gctttcgagt aaagcggcct cctgtgctat tttagcagca gcacctgtaa tgatgatggc
    37441 cgtacccttt gcgggttttt ctttatcgga ttccttagta gggtttggcc tactggccaa
    37501 tgctaaagtt gctaatgcaa ctgaaaacag caaaatccat atgccaatcc gtttgcgtgt
    37561 catcattcca atgaggttta gttagctacc tgatgttgct aagttaagcg agttaagtgt
    37621 aaacatctgt ttattaaggt taaattgaat aaaaacggtt caataattat tcattttaac
    37681 atactgaact tgaaatggta ttcaattttt agttagattt gcttataacc aaccaccaat
    37741 gccatgaaac atataatagt acccatcgat ttttcaaagg aatcgttaaa cgggttaaga
    37801 ctggcaataa tttttgccaa taggttcgac agcatggtac aaatggtgca tgttcagaaa
    37861 taccctaccg atttaacacc agggcagttc gaggaggaga ataaaaagat tgaaaaaaag
    37921 ctggaagctt tggtggagga atattctacc aagatgtcaa acagcgccaa tctatcctat
    37981 atcattaaaa agggcaaaat ttaccgcgag gttgttaacc aggcgcatgc ttttgaggat
    38041 tcagccatta tttgctctac tcatggagcc tcaggttttg aggagttttt tattggtagc
    38101 aatgctttta ggattatttc ggctacgcaa tgtccggtaa taaccataag gcatggtgcc
    38161 atgccgcgcg atatccaaaa aattatcttg cctatcgatg ttactgccga tactcgtcag
    38221 aaagttccca ttaccactga aattgctaag gtttttgatg ccgaggtgca tgtaattgca
    38281 gtggctacca tgcaaaccga tgaaattgac ctcaaactca atgcctatac caagcaggtt
    38341 tgcagctacc taaaagagca cgaggtgcgt ttccaaaagg caagcctacg tggtgataat
    38401 atcaccgata ttaccattga gtatgccctt gatgtgaatg ccgaccttat tagtattatg
    38461 accgagcaaa acattgccct tagcgatttc gttcttggca gccaagccca acagatgctg
    38521 agtagaagcc caatccctgt tcttagcgtt accccaaaag agatatttat catgggcagc
    38581 tttaaaactt acggtagcgg ctactaggct taccttgata ttttagtgac ataaacttta
    38641 ccgccaagca tttccaacga atctatagag gttgttttgt aaccctctag tttatcccat
    38701 tctaggctaa catctgtaat taacgttgcc cttgaggtaa cacgtggctg gtttatacag
    38761 tcagtgtaaa tggtgtaccc ctgcaatagt aggctatcga gtgtggcata aattttttgc
    38821 ggctcaatgt taacctcctt aacgattaaa ggtttttgaa tttgcagata ttcaagccta
    38881 ttgataactt ttggagcatc ggtaaggata taggctctcc tttcattggt attttgggta
    38941 atcgacttta taaccaaatc ggaaccatcg gcctggtaaa tcttttgtgg aagaacacca
    39001 aaggagatgt tccaaactga aacagagatg gcaaaacaaa cagtagcaag ccgttttatt
    39061 gacaaactgg ttgcagtgat aattgttagg aaaggaacca tggccaaaaa ctcagcgttt
    39121 ccacccgcca taaaggcaaa acctacctgt aatactaaag ccagcaagtg aattttatga
    39181 aatttttcaa gtctggtagg ttttgtatac ctaacaaacc aaaaagaaaa aaatgcaaat
    39241 gcaatacccg ctactactaa aggaatccac cacagccacg attgtgcaag aagatagttg
    39301 atatacccat gcatttggaa aaatgttctg aataggttga ttatgccaac cataagaatt
    39361 gtggagttca attcaattga tgcgtttcca gtgtaatagt cgctaaatat gaaccggaaa
    39421 aagctagtaa aattgatatc gatacccatt gatagcatgg caaggatgta agcggcaggc
    39481 actattactg ctggtgcaag gaaaagcagg ggtttgtaat ccctgcgatt taatttcctt
    39541 atggttccaa ttgcaagggc aatccaccaa aaaaacatta tttggtggta aagacacgcc
    39601 gatgccgata gtagtcctga ataaaccatg tttgagattt tggggctact ctcagcttta
    39661 aggaaaaggt aactagccca aattgccagc atcactggaa tagtgtatgt ttcattgtca
    39721 attgaaaacc gaaggaatcc ccagcttagt cccgcaataa tggtatagat tgtggtttga
    39781 atggtatcaa ccttaacaag tcggagtagc ttgaatagaa tgaaagcggt aaccgatgcg
    39841 caaagggcat tcatcagctt gagcaggtaa atggtttcgg catttggcag aaaaaagtgc
    39901 catattttaa cccagtaaaa tcctgtaatg gtgtatagca ggtgatgtgg acgaagcagg
    39961 ttactgctat ttacaatgtc agcagcatat ccccaaccat caattgtact caggtttgat
    40021 attccaagta tgtatattag taataggagt gtagcaacaa atgtcgcccc ccagcgctca
    40081 aaaaactttt cattcataca aacgaaaact actaagattc atgaaatctg ataaaagtta
    40141 ctatttttgt gtggttaaaa cagtgtataa aagtattgtt tttgagtaag atatcataca
    40201 ggcatgagga acagtgaaag aacaaaagta aaactactac tcgagagtgg agttgttggc
    40261 gctgaggtag cggcaatggg atgggtacgt accaagcgtg ggaataagaa tgtagccttt
    40321 gttgcgttaa acgatggctc aaccatccat aacatccagg ttgtatttga tatgacccgt
    40381 tttaccgaag aacagcttaa gggagttacc actggggctt gtattgcggt gaggggattg
    40441 ctggttgagt cgcccggttc cggtcagcgc gtggagattc aggctaacta ccttgaggta
    40501 ttaggtgaag ccgatgccac tacctatccg ctccagaaaa aaggccatag tatggagttt
    40561 ctgcgcgaga ttgcgcacct ccgccctcgc acaaatactt ttggagcagt tctccgaatc
    40621 cggcatgcca tggcgtatgc cattcacaag tactttaacg acaagggttt cttttacatt
    40681 cacgcaccca tcattaccgg ttccgatgcc gaaggggcag gggctatgtt ccgcgtaacc
    40741 accctcgatt tggagaatct accccgcacc gaggatggaa agattgactg gaacgaggat
    40801 ttttttggca aggaggcaaa gctaaccgtt tcgggacagc ttgaggccga actgggcgct
    40861 ttagccctat cggaggttta cacttttgga ccaaccttcc gtgccgaaaa ttcaaatact
    40921 ccccgacacc tggctgagtt ctggatgatt gagcctgaaa tggctttcta cgacatccag
    40981 gataacatgg atttggccga ggatttccta aaatacctaa ttagatatgc ccttgagcat
    41041 tgtgccgacg atctggaatt cctcaacaag atgtacgaca acgagcttat cgacaggctt
    41101 aagtttgtga ttgaaaaccc atttgaaagg ttaacgtaca cggaggctat taaaatactg
    41161 gaggcagcta accataagtg ggaattccct gtattctggg gtgccgattt gcagtcggag
    41221 catgaacgtt acctggttga gaagcacttc aaaaaacccg ttattcttac caattacccc
    41281 aaggagataa aggcctttta catgaagcaa aacgacgatg gcaaaaccgt tcgtgccatg
    41341 gatgtgttgt tcccaaaaat tggcgagata attggcggtt cccagcgtga ggaggtttac
    41401 gataagttac taagccgtat ccatgagctt catattcctg aaaaggatgt ttggtggtac
    41461 cttgaaaccc gcaagtttgg ctcagctccc catagtggtt ttgggcttgg ctttgagcgc
    41521 ttgctgctct ttgtaacagg aatgaccaat attcgcgatg taataccctt ccccagaact
    41581 cccaagaact gcgaattcta aaagtctatg ctcaagcaac ggttacaaca aaaacttctt
    41641 cagaagctat cgccgcagca aattcagatg ataaagctgc tggagatacc tactatgttg
    41701 cttgagcagc gtattaaaaa ggaactggag gaaaatccca ttctcgagga ggttgaacaa
    41761 acggaacctc aaacggaacc tgaaattgtt ttacccgacg ataaccagga gggtaacgac
    41821 gatgagttct ccattgagga ttacatgaac gatgacgata ttccttcgta caagctatac
    41881 tccagcaacg catcgcccga cgataagcgg gaggaaatcc ctttttccac cggaatctcg
    41941 tttcacgagc atcttgaaaa ccagctcgga atggccgacc tcaccgaacg cgaaaagcag
    42001 cttgggtttt acctaattgg caatatcgat gaggacggtt atatacgtcg taagttaacc
    42061 gcaattgccg atgatattgc cttctcgtta ggtattgaaa ccaatgagga tgagctggaa
    42121 cgaatacttt acgtaattca agaatttgac ccacccggtg tgggtgcacg cgacctccag
    42181 gaatgtttac ttattcagat taaggccaag gagcagcaaa acgagataac taaaattgcc
    42241 tataaaatac tgaagtacca ctttgacgag ttcatcaaga aacattacga taagattgct
    42301 tcaaagctta atctatccga cgaaaagctt aagctggcaa ttgatgaaat tttaaagcta
    42361 aatcccaaac ccggcggcag ttttactgac ccttacgacc aatcggcaca gcatatcatc
    42421 cccgatttta ttctcgatta cgaggatggt gagtttaggt taaacctaaa ctcgcgcaac
    42481 gtgccggagc tacgaataag caaagaatat gccgatatgc tcgagtcgta ttcttacaac
    42541 aaggagttta gcaatagcca ggaacgcgag gccatcgctt ttgtaaagca aaagattgaa
    42601 tcggccaagt ggtttattga tgcactgaaa cagcgccaga ataccctgct gacaaccatg
    42661 caggccatac tcgattacca gaaagagtat tttcgcgatg gcgacgaaag taagcttaag
    42721 cccatgatac tgaaggatgt tgctgaaaaa acagggctcg acatttcaac catatcgcgc
    42781 gttgcaaaca gcaagtacat tcaaacccat ttcgggatat tccctctcaa gcacttcttt
    42841 agcgagggca tgcaaaccga ttccggcgaa gaggtctcaa cccgtgaaat taaaaaaata
    42901 cttgccgagt gtgtcgataa tgaggataag cgcaatcccc ttaccgatga ggagctaatg
    42961 gaaatactaa aagggaaggg ataccctatt gcccgtcgga ccgttgccaa gtaccgtgaa
    43021 cagcttggaa ttccggttgc tcgcctaaga aaagaactta actaatctat ttttttaaaa
    43081 tgctacaacg actttcgctt atactttcaa ttattcttca ccctatactg gctaccacct
    43141 atatttttat agtgattttc tcgtccaact cctttgttgc ctttctacct gtaaggttta
    43201 aggtgatgct tacggtgttt gtagctataa atactatttt tctgccatta ctattaattg
    43261 ttctccttca tcgtcttaga attattcacg attatactct aaaagccagt agggataggg
    43321 ttttccccct agccatatcg gttttacctt accttttcac catctttctt ttttccaggc
    43381 ttcaggttcc atttgtttta atcaaaatac ttcaggcagg aatttataca ctgatgatct
    43441 cggcagccat ttcttacttt tggaagataa gccttcattt aactggaata ggaggtatct
    43501 ctgggtttct tttggcttcg gctctgcaag ggaaccaggc tgcagtacca atttttatag
    43561 tgagtatttt actttcaggt cttttagcct cggcccgtct tattaaaggc gatcataacc
    43621 caactcaagt ttacactggg tttcttacag gttttacatt ggtttttttg attttcttta
    43681 aataggtaag ttgatattcc tattacgcat tgctttgttt accgatgaag attgcttcat
    43741 aatattctga cctttatcat caaaaagaaa caccaaacaa ctaattgctc attttgttat
    43801 ctttgtgatt ttagaatcta acccttgtac ctgtggcagc taagaaaaag gttaaaatat
    43861 ttaagaacct gtggaataag tacaggttga gtatcttcaa tgaggagaac tatgaagagg
    43921 tttggcaact tcgtctttcc agaatgaacg taatagtttt ctttggcgga ttttctattc
    43981 tgcttattgc gcttgtgagt gtcctaattg cctttacacc cctaagggag ttcattcccg
    44041 gttaccccga tagccaaacc cggaaaggtt atgttaaaaa tgccctgaag gtcgattcgc
    44101 ttgagagggt ggtagcccaa tggcagcgtt actatgagga tatcaatatc ctgcttaacg
    44161 gaggagaacc ccttactggt cgccaaacag taatcgatac ggtaaagcct accgcaaagg
    44221 ttgagctatc cccttcagag gaggattcat tgctccgtat gcaaattgaa tcggatgaga
    44281 tgtttaacct gtcggtagga tcaagtacaa ccaagaaaag taatataccc ttttttattc
    44341 cacccgttaa gggtattatc actaacaagt ataacccttc aaaaaatcat tacggagtcg
    44401 atctggttgc cgggccaaac gagggggtac ttgccattgc aaagggaacc gtaatactct
    44461 caacctggtc gctggaaacg ggttacacaa ttgccattca gcacgaggag ggattccttt
    44521 cggtatataa gcataacagt aagctgttaa aaaggcaggg tgaatttgtt aattcaggcg
    44581 atgtgatagc aataattggt aattcaggcg aacttaccac tggaccccac ctacattttg
    44641 aactttggca ccacggagcc accgttgatc ccgctaagta cattaaattt tagtggccgg
    44701 tatgaaaaga attgcaatac taggttctac cggttcaatt ggtacgcaag cgctcgatgt
    44761 tattagggag catcccgata tatttagtgt tgagctactc gtagcccaaa acagctggga
    44821 gctcttagtg cagcaagcca ttgaatttca gccaaacacc gtaattattg caaatcagag
    44881 tcattatcaa agagtaaaag aagccctagc aaatcatccc attaaggtat atacggggaa
    44941 acagtcaata gtggaatcgg tcgtatcgtc cgaggtcgat atggttctta cagccatggt
    45001 tggatttgcc gggcttgaac ccacgcttag cgccattgag gcaggtaagg ccattgcatt
    45061 ggcaaataag gagaccctgg tagttgccgg cgagctggtt actaaagcgg ccatgcaaaa
    45121 aagggtacca ataatcccgg tcgattccga acactccgcc atatttcaat gcctggtagg
    45181 ggagcgcagc cccattgaaa aaataatact tactgcatcg ggtggtccat tccgcaacca
    45241 ttccctggat caactcatgc atgtttccaa ggccgatgcg cttaaccacc ctaactggtg
    45301 tatggggcaa aaaataacta tcgattcagc cagcctgatg aacaagggac ttgaggttat
    45361 tgaggccaag tggcttttcg gccttaaccc ggagcaaatt gaggtggtta ttcatccgca
    45421 atcaattgtc cattcaatgg tacagttcat tgatgggtct ataaaagccc aaatgggctt
    45481 acccgatatg aaactaccca ttcagtacgc attcactttc cctgagagag taagctcagg
    45541 tttcccgcgc atggattttt ccatgagtca cacccttacc tttgaaaaac ccgataccaa
    45601 taggtttcca agccttgccc tggctctcga atcgttaagg gcagggggta atatgccctg
    45661 cgtgcttaac gctgccaacg aggtagctgt tgatgctttt ctgagggagc agattagctt
    45721 tatggcaatc ccaaaggttg ttgaaggtgc tatgcaagcc ataccgttta ttgggcaacc
    45781 tacctttgcc gattacattc aaaccgattc cgaaactcga ataaaatcag ttgaactcat
    45841 taataaaaac atcataaaat ggagatactc ataaaagctg gtcaattctt tttaagcctt
    45901 tcaatcctgg ttattcttca tgagctaggg cactttcttt ttgcaaagct ttttaaaacc
    45961 cgtgttgaaa agttttacct tttcttcaat ccctggttct cgctttttaa aatcaagcgt
    46021 ggtgaaaccg agtacggagt gggttggtta ccactgggtg gttatgtgaa aatttcaggg
    46081 atgattgacg agtccatgga taaggaacag ctcaagcaac ccccacagcc ctatgagttc
    46141 cgctctaaac ctgcatggca acgcttacta ataatgattg gcggagtgct tgtcaatgtt
    46201 attctggccc tgttcattta tagcgcagtt ctatacacat ggggcgaaga ttaccttcct
    46261 acgaaaagtc ttaaatacgg tgtttcctgc gattccttag ccacagagtt aggttttagg
    46321 aatggcgata aaatacttta tgtaggtggt aaggagattg aaaactttca gcaaattgct
    46381 catgatattc tgctaaatga gaatcgtgac gttaccgttc tacgtggcac cgatacattg
    46441 aacattaaaa tagctgagga ttatgtggct aaacttgtta aaagcccaat tatttttttc
    46501 ccgcgcatcc catttgtagt agatgatgtt aagtcaggag aacctgctga aaaggcaggg
    46561 tttatcaaag gcgataggct gcttgcctta aacggacaat ctgcacagtt ttttgatgag
    46621 ttcaaatccg aaatgcagcg tagtaagggg aaaactgtaa gggtaaccgt tgagcgcaac
    46681 ggaaacccta tcgacctaaa tgttgatgta cccgaaactg gcattatagg ggtttaccct
    46741 gttgccgatt taggaaagtt ctttgaactc agccacaggg aatattcgtt ctttcactca
    46801 atacctgccg gtgttaaaaa gggttttgca accatatcga gctacttaaa acagcttaag
    46861 ctaattttct cgcccaaaac caaagcatac gaatcggttg gcgggtttat tacaattggt
    46921 aaaattttcc cctcaacttg ggattggcag gcattctgga gcttaactgc attcctatcc
    46981 attatcctcg ccataatgaa cattcttcca atacctgcgc tcgatggtgg ccatgtaatg
    47041 ttcttgcttt acgagattat tactgggcgg aaacccggcg ataagttcct tgaatacgct
    47101 caacttgttg gcatggcaat ccttttagcc ttgcttttgt atgccaacat caacgatatt
    47161 gttaaacttt ttaggtaaaa gagttctgta tgctaacact atttattggg ggtggagagc
    47221 tgcttgttat tgccatagtt atcctggtaa tttttggcgc aagcaaaata cccattttca
    47281 tgcgtaacct gggtagaggc gtaggtgagt ttaaaaaagg tataaaagaa gccgagaatc
    47341 aagaggataa agaaaaggaa tgatttatgt ttttaggatt tagggggtgt cttttatcac
    47401 ttttctcact tgtcttttta aacgggtttt caaaccctgt taaattaaat aggggacaaa
    47461 accctatagt gtctttcatt gatgctattg aaaatcttcc cattttcgtc cccacaaatc
    47521 aaaccgacac agctaggcaa aaacctgacc tttactttga gtgccagctt gcggagattg
    47581 gccggaactc gcccattgat tttgattaca atccccatgt aaggcgatat atcgaaattt
    47641 ttaccattga gcgtcgtgag caaatttcta aaatgattgg cttagccaat tactacttcc
    47701 cccttttcga tgatgtgttc agcaaatacc agttgccgtt ggagttaaag taccttgcag
    47761 tagttgaatc ggcattaaac ccattggcaa tatccccaac cggagcagta gggttatggc
    47821 agtttaaaat taatacatcc aagatgttta accttcaggt aaacaacttt gttgatgagc
    47881 gaatggaccc cttgaaatct actgaggcag ccgctcagta cctgcaatac ctctacagaa
    47941 cttttaacga ctggagttta gcactggcag cctataacgt tggacctggg cccattagga
    48001 atgccattga gcgagctaac ggcgaaagga atttttggaa gctttatcca ctcctccctg
    48061 aatcggcgca aaactatgtg ccggcattta ttgccgctgc ttatgtaatg aataactacc
    48121 atgaacatgg cattacgcct gctcctgtag ctttagctca aaaccaaacc gatacagttt
    48181 gggtaaataa acccctcgat cttaacctta tggctaaggc cattgatatt gatatcaata
    48241 ttattcgctt tctaaaccca acctatcgtt acgattatat ccctgagggt tcttccccac
    48301 aggtgttacg gttgcctgct gataaaactg gattatttat caggaaatca aaggagcttt
    48361 acctgaacac atcaaaacct aagattttgc ctgcgttagc tgccgatagc actttaaccc
    48421 gtaaggttta caccgttaag aatggcgata gcctgcataa gttagcaatg cagtttggtt
    48481 gcaccctcca ggatatttac aggtggaatc ccggattgga tagcacaata actgcaggcc
    48541 aaaacattgt tatttgggtt aaccctaaaa atctctgaaa ggttttaggc gttagcaata
    48601 aatattgttt ataggcgttt atcttgaaaa tcaaaactaa tattatgaaa agtaggtgga
    48661 taataatact tgcggctgca gttgcactta gcccatcatg caaaaagggg agcaagggtg
    48721 agcggtcgct tttacccaat gtaaccggaa gtgctaatga gcttgtggta atactgccaa
    48781 aagcacttca tagcgattca ctgggtaagc tgtatagcga tattttaact gaggatgttc
    48841 cctttttgcc tcaaagcgag cctaggttcg atatggtgat gattccaccc gatgcgttta
    48901 ccgatatctt taagccacat cgtaacatca tttttaacga tatagttaag ggtgccaagg
    48961 aggcaaaaat gtctcttaaa agagatgttt gggctgcacc tcaaaccatt ctttacatct
    49021 cggggcctag ctataaatca atttacgacc tcctactaaa ggagcgcgat aaagttgtaa
    49081 gcatttttga gcaggctgaa cgcgataggg tgatacagaa tgccattacc tatgaggaaa
    49141 agcccattag ggaagcactt gaaaagaagt atggtgtttc gatgtatttc cctaaaggct
    49201 acaggctgaa ctttgaccac gacactattg cttgggtatc ctatgaaact ccgcttacaa
    49261 gccagggcat tattctttac actttcccct atacagcaaa aaacaccttt acggttgact
    49321 accttttggc taagcgcaat gagtttgtta aaaccattag cggtccaact cagggttcgt
    49381 atatgactac cagtagcgtt attgttccgc agcttaccga tatgatgttc aaaaatcgtt
    49441 attacggagt tatgcgtggt ttgtgggatg tatataaaca tccaatgggt ggaccattca
    49501 tcagccatat tactgttgat gaggaacgcc aaagggttat tgttgctgag gcttacgttt
    49561 atgctccatc aaaggagaaa cgtaattacc tccgacaggt tgaggctttg ctctacacca
    49621 tggagattat taaaaagtaa atcttcttaa ttaaattatt tttgatttaa ctaataaaaa
    49681 aaggggtgca gagaatgcac ccctttcctt tactttgcga gtgtttattt aatagccgta
    49741 tcaaccagtt catcaacaaa ggctttaatg gaacgtgaat cggtagcacc ataaaagtcg
    49801 tagaagtatt caattaagta ttttttgatt tcatccttac ccattttctg tttaacaagg
    49861 attggaataa tggtcataaa ctcattggtt acagtttcct tgtcaacaaa ggccagatta
    49921 aactttgtaa tctcctcgct ggtttttgtg ttcctttcgt aaaggtaaac tgcaaccgaa
    49981 ggtatctcgt tttgggttat tacgccggca tttgtgttaa caagcttatc gcgttcaatg
    50041 aaaagcgttg tgttttcata cccaatcttt tttgaaaccg gattgttatt cacccggtag
    50101 tagaatacca cgaacttatt ttcatcaatg gggtaggatt gggggttaat tggaacagca
    50161 atattatcgc caataatggt gaacttgtca ttgccaaagt aggaacggag gtcggaaacg
    50221 gtggtttcat cggggcggta acgctcagcc ataaggcttc tgcgcttggt gggaacagca
    50281 atatccttaa ccatggccga tagctgttga atggtttcag gagctggtgg ctcatgcagc
    50341 ttaatcatgt aacgccccat gttttgtcca atggaaatta cgtaggagtc aaacgagtcg
    50401 aacgttacct tgtccgatgg ttgaagcaca tcgccagctt taagctgagt tcctgttttg
    50461 ttgttggtta ccacaccgtt tattcttgta acaataaacg attcctgtgc ctgcacacta
    50521 accgatatgg caagcaaaag gactgttaaa aggtgaacta ctggtttcat ggctaaattt
    50581 tttttaatta cataatcaaa taatatacca aagttacact tttcaaatta acccttaccg
    50641 tttttccatt aaatatcact actatccaac taaaatttta aaaattactt taaccctggc
    50701 ttagtctatc ctccatgagg aatagaaaaa gttagagttg gttttattgc aacgcttcat
    50761 cgcgtgtgga tgccagcggt ttgtcattcc gagctagtcg aggaatctcg atttatttaa
    50821 gatttaaaat ttaagattta agaactcacc agttatcaca cgtctttgga cacgcacttt
    50881 aatcgccctg aaagggcaaa tcagctaagc ccagggtttt aaccctgggt gttgttgtat
    50941 tcaaaatttt gggcgatgca cgcaatacat attccaaaga gcaacactga aacgttgcat
    51001 cgcaacg
//
LOCUS       DAOSAL010000009        50738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_513_length_50738_cov_35.636860, whole genome shotgun sequence.
ACCESSION   DAOSAL010000009 DAOSAL010000000
VERSION     DAOSAL010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 50738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50738
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_513_length_50738_cov_35.636860"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            227..946
                     /locus_tag="PK049_02460"
     CDS             227..946
                     /locus_tag="PK049_02460"
                     /inference="COORDINATES: protein motif:HMM:NF024063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HOK60449.1"
                     /translation="MDILIIIIGLFMAALGFLAKAYPNLIAGYNTMPRDKKKNVDIEG
                     LSTLIRNGLAITGLTIIVSYFVFKWLGFTTVANLMIVIVTLAGVTTIAVKAQRFDHNK
                     SKSKLHILVLGAAIIFVASLLTYGFIPTKVIYGNNSVRFSGMYGITVNIDEVASVELA
                     NAIPKIKLRTNGFSVGTVNKGTFIVDSIGKCRLLLNSGSKPYIVLTKTSGEVCIINLK
                     SSSQTHDAYQNILKLVSQGKK"
     gene            1124..4234
                     /locus_tag="PK049_02465"
     CDS             1124..4234
                     /locus_tag="PK049_02465"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOK60450.1"
                     /translation="MIRYICSLMLMLTISAGLYAQRTLTGIVVDAENGEPLPGATVVV
                     DGTTRGTSTDFEGKFSIELGENEMFLMVSFVGYDKKRVNVGNQATIRIELNRESTVLQ
                     DVIVTAIGIKAEKKALGYAAQDVKGDELSTTNQSNIVNALSSKIAGISVTSSSGTPGA
                     SADIVIRGRSSLRADGNSPLFVVDGVPIDNDYAGSAVYDYSNRAIDLNSDDIESVSVL
                     KGAAASALYGIRAANGAIIITTKSGKGKGIRRNITFKSTLGFDRVNKLPEKQYRYAQG
                     TNGSYSSTTNFSWGPLIDTLRYDGDINYPYDKNGRIVGMSDPSATDKRVIPYKNIDDF
                     FETAVNSNTYLSISGGSDNGSYLFSMGYLNQNGVVPLTSFERLNFKLSGDTKLSDKLK
                     ISGSATYSNSQGSYAQKGSNLSAVMVGLMRCTPTFDLTNGSSDPVNDPSAYMFPDGTQ
                     RNYYSKYDNPYWSVNKNRSSDEVNRIIGNSQIDYQILPWLSAMYRAGIDFYSEQRNSF
                     FDNNSNDVTNGYVTASLYNFNSFNSDFILTAEKDITNDLKLNVLLGHNYYNKYTYLNS
                     QRGDSLILPNFYDLSNTAVVTGDDYISRYRIVGAYYDIRLSYKNYLYLNTTGRNDWSS
                     TLAKGKNSFFYPAVNGSFIFTEAFNLKNKIFSFGKVRASWAQVGNDAPIYSLQNYYSA
                     ISGGINGQTSFATQRTIGNNNLKPEETRSWEIGVDLKFLDNRLGLDMAYYSSTTDGQI
                     VEVPVPYSSGYDRMVLNAGIITNRGVEAQLYATPLKMDKFTWDMQVNFTRNRNIVEDL
                     PEGIPLLEFQNTGLSSTRSVAIKGEPYGVLYGTRYLRNDKGQILVSNDGYPLVDPVPG
                     VVGDPNPDFTMGIRNTFTYKEITLTALVDIKKGGDVYNGTKNVMSYLGTHKETENRDE
                     LYVFPGVNVNTGEPNTVAVKRNFDYYSRLGTMAGLSEAGVEDGSYIRLREIAINYSLP
                     TKWLNRTPIKGLMVGISGRNLLLFTKYSGIDPETNLSGTSNSLGRDYFNMPNTKGVEF
                     NIQVNF"
     gene            4247..5626
                     /locus_tag="PK049_02470"
     CDS             4247..5626
                     /locus_tag="PK049_02470"
                     /inference="COORDINATES: protein motif:HMM:NF024181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HOK60451.1"
                     /translation="MKKVNKTIKITAIATLLLMVSCEISEVNINPNSPTQVPENVILP
                     FAEESIARLMAGSGQVMSGIFVQYYEGIDNHPRQVQQYVVNEALYVEWDWNDYYDGPM
                     INLKKMIEVAKGNGNFYYVGIGNTLLALCLGNVTSLWGDIPYSEALDGANNKSPKFDT
                     QKSIYEAIQTLLDTAIVNFNKSYNGRKPTTDDILFGGDIAKWKKAAYALKARYYMHLT
                     KRAAELDFNPAQKALEACQNAISSSSDDMLFPYGYSASEYNPFYSFTRLGYIIPNISF
                     TSLLSALNDPRKDFYYKKKFGLANLENSYFTSPNSPVFMITYHELKFIEAEARLRLDE
                     NDPLAQTALQQGVRANLNKICGDAVPQATIDSYVTANANLTGDFENKLKTIIRQKYIA
                     MFASIESWTDYRRTGYPVLTPNPNGDNPQNPGGAIPRRLAYPQTERLYNKNFPTPLPT
                     LQDRFWWDE"
     gene            complement(5682..7028)
                     /locus_tag="PK049_02475"
     CDS             complement(5682..7028)
                     /locus_tag="PK049_02475"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOK60452.1"
                     /translation="MRKILISILLIPFIACDDWLDIKPKNYVASDEVFTTRENLYAAL
                     TGCYDALQLQHYFGRSFIIAGDLLSDNSVAKGTKIELIALDENNLTPDNIIVEGVWED
                     IYTAINRANYILEGIQDVNFLNETEKNDIVGQLLFLRALHYFNLVRLYGAVPLKLKPT
                     TKNDPSNFLPRSPIGSVYQQIISDLDSAEISIQNIQPQWATVRSSRALKALVYLTLAD
                     YPHALQYANECLLENSYLESDYSKLFGSYTEPSAEIVFYIPFIPSDNNRLAEYHFPNQ
                     LGGRYENAPKSDLVSLIAPSDKRKRWIAESLSTSSGIISYTKKYPNLSTGANNVTVLR
                     NAELYFIRAEALYFTDSVAHFNTILNDINTIRQRAGLNPIDSATALGKLWPILENEKQ
                     LEFAFEGKRWFDLVRTNRALVKIPSITSVNQTLFPIPQTEILYNPQITPADQNPGY"
     gene            complement(7013..10198)
                     /locus_tag="PK049_02480"
     CDS             complement(7013..10198)
                     /locus_tag="PK049_02480"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOK60453.1"
                     /translation="MRPILSTLAILLFFYTLCAQDVAVYSFSFKDQKLGDILKDISSQ
                     TGYKFAFSSSDLNVDTIVTFSARKKAAADVFNLLGKTIECSVEIINNTVVLKPLRLMP
                     PFTLRGTVVDFSTGDFIAGANVITSDMKGTYTDSAGQFSLAVKYGQTLNFSCIGYHPL
                     NYQVTTDTVVKLWLKPMLNPIGEIVVVAFGSEQRNLLTGSVSVVDPQRFSQVNDASVM
                     GAFQTNLTGVLIQNNAGTPGSSVKMNIRGITSISAGSSPLFVIDGVPVITGNYSQLDF
                     SGQTIDAISDIPVNDIESITVLKDASSSALYGTRASNGVVLVATKRGQASANSISVDS
                     YFGLQQANRKLNMLNATQWMNMINEEAIQRGEPPVYSDDFIQNNKVDTRWLDEVFRLA
                     PTYSLQLSFRGGDSNSSYYVSGNYYNQDGIVLGSDFSRYGFRVNYDYSINPKLKLQVG
                     NGFSFSTNNRVEGDQSLNGPLPNAISMPPIYPVYNPDGTFNNDGPYANPISIAKEEKN
                     LAKSHRNISNLTLTYKPSSNFQINAQGGIDYYNLNEQTFAPKQTRQGAKYDGLGIEAT
                     SNVITFYYSSYANFTKKWKTGNLEVLGGLSTERYMEHGSYLRSQSFPGNSIEFLQGGA
                     IPVQASSREVDALNLSVFSRLKFLYLNRYMFTFNFRRDGSSKFGESNRFGNFPAIAFL
                     WNAYDEPFYPKHTLFPRVKLTLSYGLTGNDQISDFRALDLFAPGSNYGGSAGLQPAQI
                     ANPNLKWESTHQFNAGIRVDWLDRVRLSADYYVKNTFDLLYRLPYPTSTGFSHIISNI
                     GKMRNSGFELDLNADLLTELVSWNVGFTITMNRNEVVSLYQNQPIRNIGRASSSIEEG
                     QPLSFFYGYKALGVNPADGMMIYKDLNGDGIINDFDKTRIGSPFPDLFGALTSQLTYR
                     SFSINFNIFYSIGNEIFNATRMYTETLSKSNQTTAVLRRWRQPGDITDIPKESTYNKL
                     ISSRFVEDGSFVRLKSIKLTYTLNQKVIRKTAFAGLQFYIAGKNLFTWTRYSGMDPEV
                     NYNGENSIVMGTDFFTCPQPKTLILGVCAKF"
     gene            complement(10319..11710)
                     /locus_tag="PK049_02485"
     CDS             complement(10319..11710)
                     /locus_tag="PK049_02485"
                     /inference="COORDINATES: protein motif:HMM:NF015563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60454.1"
                     /translation="MSKKIVFPHTIVIIIFIMIGLILLTWILPAGEFEKQLVNGRKVI
                     VPGTYHQVSGSPQGLGSMLTAPIKGFIEAAQVIGFCLIVGGAFGMLARTKAIPAALNR
                     LLERGKDNPRLNSIIVPIVMVFFSLAGATFGMSESTLVFVMITIPLAIALGYDSIVGI
                     SMSFMAAGVGFAAAITNPFNIGVAHGIAELPLFSGWEFRVLIWTVLTSIAIFFVMRYA
                     KRIKRNIQISSVYEIDRDRSVEISEDSSIKIFDIRQKIIIGLLFISLILLVYGANQWK
                     WFINEISALFIALGVLSAVVYRLKTRDAVDAFINGAKDMVTAGLIIGMSRGLLVIASQ
                     GKIIDTILFSVSGLGKSLPPELSVQVIFIFQSLFTFLVPSGSGQAALTVPIVAPLSDL
                     LEVGRQTAVTAFHLGDGLFSMLVPTSGVTMGVLSIAKIPYTTWVKWFIRLLLILFGVS
                     MILLVFALYYFGK"
     gene            complement(11721..13703)
                     /locus_tag="PK049_02490"
     CDS             complement(11721..13703)
                     /locus_tag="PK049_02490"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOK60455.1"
                     /translation="MKTITKSLSFVFLMLVTLNYAKGQGFLMLAGGGGESSGGWSDLP
                     YSWVVTQSANKKVAIISVNDETDWLPNYFKSFGAKWAKNFKIDSKLLANSQWLYDSLI
                     TYGAIFLKGGDQSKYYEYYKGTKTQQALQQVFSSGGVLAGTSAGAMILSPIVFTAQVA
                     SVDPSTALMSAYSSQITLANDFLNTIQGNFIFDTHVAERGRLGRIPAFMASWYKQTGQ
                     LAVGVGIDDHTAICISPNGMATVYGTGAVGIYRVNSATNPFDTQVSMLKSDYIELTQL
                     LHGTTINLNTFEVSGYSNTINPLVNEINSRITVFFSGTDYPSDDACAYFVNQAGGSAD
                     PILIVTGTDQTRANSLKSTFTAKGAAKVDIIQGLTANSSNTEHQALINAAKKFVFVSN
                     DRVELFSYLNAAGNGLLLKTKLKAPEMVSFFVGDNARFAGASVVNNYTTSGASYNGQL
                     QFSSGIGLLENLCIIPNAFINSQYYENCVTGVSYLMLRDKLKNGLLITGSTFACNTFD
                     SQQKSFLRNVMGSFPLVYFSNTGTRGAFANQGPYSQSRNIAGFERVMLRFLGTADTVI
                     LGNNVPTYTPENQYLGMLIKVYPNPAIDYVSIEVLNPGILEIFDVTGKKVMESRLTET
                     STISISNLHDGIYFLRFRDDRNVLVTTQKLLKLSKN"
     gene            complement(13713..14489)
                     /locus_tag="PK049_02495"
     CDS             complement(13713..14489)
                     /locus_tag="PK049_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60456.1"
                     /translation="MPRFWFISLLLLLFSCSQNIEPKNGSITLYYLGSNQFQNIDIPQ
                     KNINLDNSIILYSDSKKQKAEILYSNLNLKNWKVKKYSINQTINSDFSLNQLIFLLID
                     ASKRNDILYISNLLQKKSVKGKIVIADGCADEIFFRKIAHPSNNSVLLTEKVGVNDII
                     ISNGFDLLELPIALVKSNSPNRFSEMLSVAMQNPLDYIVSVDDSAYITIQRNVIKVYG
                     KGQMFMVKHPEAITKVDNGLFGGENLQVSVFLPGDSLVIK"
     gene            complement(14557..15090)
                     /locus_tag="PK049_02500"
     CDS             complement(14557..15090)
                     /locus_tag="PK049_02500"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HOK60457.1"
                     /translation="MSREKQIALKVIEGNHDAFAQLYDLYAERIYYFALRFMISKEDA
                     ENVTQEVFVKLWETRDRIDVNLSLSSYLFTIARNTIFNIHRKRVNEIAYLEHLSTYLE
                     SNHVRLENEIIFRDIQEQLERIINDLPPQRKKVFDLSRKQGLSHKEIAEQLNISEKTI
                     ETHIRLALKTLREGLDQ"
     gene            complement(15110..16282)
                     /gene="iadA"
                     /locus_tag="PK049_02505"
     CDS             complement(15110..16282)
                     /gene="iadA"
                     /locus_tag="PK049_02505"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HOK60458.1"
                     /translation="MSMFTLIANAEVYAPDYMGKKDVLVCNETIIAIDDSINPKILPD
                     NEIDIIDATGLKLIPGLIDAHVHIAGAGGEGGPSTRTPELQLSQLIAGGITTVIGCLG
                     TDGITRSPEAVLMKAKSLRAEGVSAWMYTGSYQVPTPTITGSIARDLALIDEVIGAGE
                     IALSDHRSSWPTTAELIRLAEEARVGGMLGGKAGIVNIHMGDAKNPFQPIHDAVSQSE
                     LSYRQFHPTHCNRNDYIFEDAKKYGKEGYIDITASSYPYFPEYEVKPSRAIVELVRAG
                     VPLEHITMTSDANGSLPLFDKNGELVRLEMGQPKSIYNEMIDTHLKEGLPLEKALTVV
                     TSNVARILKLKSKGKVQVGTDADLVCINNDFAIVHIIAKGKILLREGVARAKGTYE"
     gene            complement(16286..17275)
                     /locus_tag="PK049_02510"
     CDS             complement(16286..17275)
                     /locus_tag="PK049_02510"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOK60459.1"
                     /translation="MSLRLKAKQLKRMMLGIASEKEKQEIYNSHESNSMLEQVWNNTN
                     SELSQPSFDKQKLFQKISNQIDNNNVPTIRWYQKPSFRVAASIAVLVTISLSAIYFVW
                     NSRNFAEKQMLTVFSGSNSMQQVVLPDGSKVLLNKHTTIKYPKVFSKKVRRVELSGEA
                     IFEVTHNPNQAFIVAANNVEVEVLGTVFNVMAYPADGMVTATLISGKVKLKYIDLSTK
                     KEQSVTLAPNHSATYYFNQNRFEVSMVDVSKVTAWEQGKLIFSDEPIESVVNKLNRWY
                     GVEISLSNDLKGKYRLTMTVDNETIDEVLLIISKTIPIGYSKSDKQVSIYPKQ"
     gene            complement(17419..19944)
                     /locus_tag="PK049_02515"
     CDS             complement(17419..19944)
                     /locus_tag="PK049_02515"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5018 domain-containing protein"
                     /protein_id="HOK60460.1"
                     /translation="MKSKILLTTLFVAIVAATFAQPLYWREDLYDPTKTSTITRDNSV
                     KSEGDYSLKYVFTDDGTPYFICDTFAVTGGANYEFKIDYLDNDPACKISARLWFFSAP
                     NTSYLERQTSGATTDSGDWQTITISGTAPAGATLAYVAIRMGTDAAWAGSGTFWADNA
                     RYFENGGTTNLIKNGGFEGWKVVENSTIKNWREDLYDPSKTSTITPELAKSTHGFYSV
                     KYTFTDDGTPYFICDTFAVTGGATYEFNIDYLDNDPACKISARLWFFSAPNTSYLERK
                     TSSATTDNADWQTITISGTAPAGATLAYVAIRMQTDAAWAGSGTFWADNARYFENGGT
                     TNLIKNGSFEEWEAPTEPEFLTYKFEGLNPVVNGAIDKTAKTVTATVPFATDLTNLVA
                     SFTTNGATVKVNDVVQESGVTPNDFTNPITYKLELGSVNTNWVVTVSKAAPATGKDIL
                     SFRFEGLTPPVTGIINAAEHKVTAEVPFSTDLTTLVPTITVSQYATISPASGVAQNFT
                     NPVTYTVTAQDGTTQAWEVTVTKAAEGRTTLLFEDFENKILIPSGWVLINGDGYTQAP
                     GEERWQDSAWVVATTNRQELQGTQVAMASSYTSDMPMDGKAYDWMILPKVDLGNNTTI
                     AWQAMSTTTSGNYPDDYMVVIAPAVDGVTPTISYFETEGNILLEVKPESWSQYVGRPG
                     QGLATRSINLRNAKTVSAPDGWFNRPVWIAFVLITDLYVNPDTGIPNSSPGGSNLAVD
                     NIHIYNDVLSGISDNAKNLLNTLVYPNPASEKATILVPTQLGGKVDVVVTDIVGRMVY
                     STSINATENQTRVNLETKDWGNGIYVVRTTYRGTTGINKLVVR"
     gene            20147..20995
                     /locus_tag="PK049_02520"
     CDS             20147..20995
                     /locus_tag="PK049_02520"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0044265 - cellular macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HOK60461.1"
                     /translation="MTGKNILLYLLLGIVSLSFLKCTQQSASKGKLYIIGGGKRPDAM
                     VQQIINLSKLKEKGYLVVLPMASEEPDSAAYYATKQFTDRGINSALSIIFQKGDTIKQ
                     WWVDTLKKASLIYISGGDQKKFMDIVGGTPIHEALVYAYHNGAVIAGTSAGAAVMSKL
                     MITGRQLKFPDDERYGHLVPNNIEITDGLGLVKNIIVDQHFIKRERLNRLIAVSIENP
                     DYLCAGIDESTALLISGDSATVCGIGQVVLVDGSKSKAISSDSLLGAKGLKIDVLLPG
                     ERFKLP"
     gene            complement(21090..22214)
                     /locus_tag="PK049_02525"
     CDS             complement(21090..22214)
                     /locus_tag="PK049_02525"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOK60462.1"
                     /translation="MTQKVGEITALSDDSLQKLIREYRAIDMTSLTPNEKGFQEFIDS
                     NINDYNLDMDRDESMNPYQKLSKYILKKYFRAINPETEIAFVSGVNLAVFLAIASTVK
                     DGDSVIVFEPFTSNLKTAVELCGARPVYIPLRAPDFRIDWCEVQRAINATTKLMVISS
                     PHLLTGQTLTAEDFEELQRLINGTKIKLILNESMAELGFSSSEGTSVNFYPRLCQVSY
                     RIGSLNIPVALNDSEIAYCIAPEPLMGAYKNIHSAITSNPNLNRAEDFINILSAEIEG
                     HMLADFLDENYKLLLETLKDSKLIPVKQSAGYMVMLGYKGYADIRDVEMVEKFIAEKG
                     VGLLPLSGFYHDRQCHRYLAMNITIPRDEFKEALSRLASL"
     gene            complement(22313..23041)
                     /gene="recO"
                     /locus_tag="PK049_02530"
     CDS             complement(22313..23041)
                     /gene="recO"
                     /locus_tag="PK049_02530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOK60463.1"
                     /translation="MLQHNRVIVLRTVRFKENSLIVSCYAREHGRITLLVNNAFPKGK
                     KAGLNVYFQPLSILDVVYYYRPNAEMYRVKEVTPTYAFTSLHLNPIKLTITIFLSELI
                     YRTVREQETNQHLFAFIESSVQMLDNLNTGVANFHLIFMVQLSRYLGFYPLNRWSPEE
                     PYFDIKNGVFCGVEPAHGFVLEKQTSKLLSNLIDTPMLNPEMLSLNQNQRQILIEAIA
                     SFYRFHLGSGIRFRTLPILIQLFE"
     gene            complement(23046..25346)
                     /locus_tag="PK049_02535"
     CDS             complement(23046..25346)
                     /locus_tag="PK049_02535"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOK60464.1"
                     /translation="MVKRIYILLSFIFVGICKVHTQPAIGQWRDYYAFNDARELSISG
                     SKLYCAVANGYFTYDTENGELNKYTTVSGLSEVDITALSAIPQKQITLVGYKSGNIDL
                     VYENTKRVVSLPFIKDKPMLGSKCINHFYYFSSLVYVSTDFGLVVIDPDRLEVKDTYF
                     IVENAGTLKVNRLVAWNGKFWAATSQGVYSASTNDPLLISYERWSREQFFTDPAAECR
                     GVTADETHLLALEKVGGNDILWMNSGSGWVEVDRPFDETLGVSIGFQKIAAFSSNAIQ
                     KYSLQGEKLELVSSYLFGGTPKVADCIPLENNALAVADEFNGLVIISPTDHSVHTPTG
                     PFSNKFFHIDASANMVVAASGAYDAAFGNFWNPFIAHTFSDGRWSYYADWFNHDAVRV
                     KADPRNPNVFFVASWGGGLYKLNSGTLAEHYNPDNSTLQSVFPGQPYCRIAGMDIDPK
                     GNLWVANSEVPNPISVLTAQGVWKSFPYYSAIGTSRIISLTVSPSGIIWLALARDNGL
                     FVLSPGNDVEAASDDIYLKFRPRDANGNLFSNEVTALAFDRDGYLWLGTNQGVLVSYN
                     PHRVLQGETSFQKIKIPDVVEGLAVYLLETEEVTCIDVDGGNRKWFGTAKSGVYLFSA
                     DGTKLIHHFNTKNSPLPSNTILSIKAHPVTGEVFIATDKGLIAYRSNASEPANTFDKV
                     YAFPNPVRPNYSGVITIAGLMDNSVVKITDIAGNIVYETRSNGGLATWDGKNRNGQRV
                     ATGVYLIFCSDSKGEQAAVTKLLFVK"
     gene            complement(25410..26003)
                     /locus_tag="PK049_02540"
     CDS             complement(25410..26003)
                     /locus_tag="PK049_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP diphosphatase"
                     /protein_id="HOK60465.1"
                     /translation="MAGSSFSLVFATNNPHKLKEVQHALGDRFTLVTLNAVGITDEIP
                     EEYGTLQDNALQKARYVYNKTGQNCFADDTGLEVEALNWEPGVYSARYAGDAKKPKDN
                     IRKLLSNLKGVENRKARFRTVIALILNGEEYLFEGVVWGKIIDQERGADGFGYDPIFV
                     PDGFTETFAEMPLSLKNQISHRGKAVEELCKFLKSRF"
     gene            complement(26011..26805)
                     /locus_tag="PK049_02545"
     CDS             complement(26011..26805)
                     /locus_tag="PK049_02545"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOK60466.1"
                     /translation="MTAKKSILVPTDFTEVAWFALQHAIRVSEVVKEPIHILHCVSEP
                     EKLAEAESKMASLIESVKSKYAVTPKSIVKVGNYMTDIASTADEINASMVIMGSSTVK
                     EMDEHKVSWVLKLITSCKVPFITIQEPPVNKRYDDIVFPVDFTIENREKHEWISYFCD
                     YYLSRFHIIKPNTSDPKLMAQIDINLASAKKFLDERGAKYVEYTVPGIKKYEEEVLDM
                     AVNIRADLIIIMTTPTSKDGKYIVEPEEQYILANARHIPVMSINPR"
     gene            27044..28435
                     /locus_tag="PK049_02550"
     CDS             27044..28435
                     /locus_tag="PK049_02550"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOK60467.1"
                     /translation="MMLYVRYYYTKKSFALQQKLFVRTEELLRERQKTENLLSRVLPR
                     EKAKELIDQGKVDAQKFQMVTVLFADIQGFTKITDEINADSLIDQLDRLFFEFDSIVE
                     KYGIEKIKTIGDAYMCAGGIPHPNRTNPFEVVAAAVEMMGCMQKLNQQLNNGKEIWEL
                     RIGIDTGPVIAGIVGRNKLTYDIWGTTVNIASRMESASEPGKINISENTYLVVRDLFE
                     CKFRGKIPIKNKGDVNMYFVEGFLPKFSESSRYIPNNDFRVELQLVRLADLEEFVLEK
                     LEKGLPKNLYYHNLKHTVDVYTQVELIGRAENIPNEDLLLVRTAALFHDMGHLIDYAT
                     HEEMSVKMAHEILPRYGYTPEQIARVAEIIIATKLPPKPKNILEEIICDADLDYLGRT
                     DFIPVSNALYKELHEHGMVGSLHDWNLIQIDFIQKHQYFTQTARRLRNVNKDVQLKNL
                     KRWMNELENDTSL"
     gene            28531..29370
                     /locus_tag="PK049_02555"
     CDS             28531..29370
                     /locus_tag="PK049_02555"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOK60468.1"
                     /translation="MDNIKKIILVPWDFTEGSEIAFQHALQLAKAGGNEIMLLHIIPR
                     AWIIDSIYRKVNFKKIETYNAKIEKAASELEHKYGFKPQTLVLEGYPKRIIKELILTA
                     HVNLIVAYYAYKFGSKEHRISRYLKNLFFKDTTLPFIVVSKAPIHSHYIEIVIPVDYD
                     RKFKEAVHWIIYLAKYYKCNVNLIKPFLTDDGKKKQLANNIYFTKKMLDGVNIVYGIK
                     TAKKKGTFEEEVFRFARTIEADLVLIMSDKYDDFLDSKQEAKHDIPVMCVNPRIRKYQ
                     SFY"
     gene            29416..32196
                     /locus_tag="PK049_02560"
     CDS             29416..32196
                     /locus_tag="PK049_02560"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOK60469.1"
                     /translation="MKLSLKEKIIFLFTGTSFLILFFLLFLNSARFNRLSIETAKSRI
                     SLLADKEVKKFEATLERDMSVARTIASSMAVTIKEGKTSNIQEYNNLYTNILEQYPDV
                     LSVWDSWELKFVEPGYTYEHGRIKNYAWREGGKILANTIRTEKTESSAEYITLKTNKS
                     ESLEEPYFDNYEGQTENILMTSVIAPCIIDGNFAGVAGLDIPLSNYHQVVKSIVPFKD
                     SYAFIISPQLKFIAHPDKDLIGDDALFSYESIFTSHGVQDKVINGEPIEFEATDINGK
                     LSFFTFRPISVGKCPQHWSFVLITPRETILAEARHVFWTTLTIGLIGILLLSGFIYLM
                     TLKFLINPINKVTQNLERLSKGHVDNSMIVDVDNNDEIGTMITHLNKTVEGLYKKTEF
                     AKQVGMGNYDTSLELLSDEDVLGKSLIEMNENLRKAREEALIRQRDEEKRRWVNEGLA
                     RFGDILRQNNNDIEALSASIVKNLVGILNANQGGLFIYNDENTDEPYFELTAAYAYNR
                     QKFIKKHILLGEGLVGTCALEKQTIYLKEIPENYIAISSGLGEAKPRNLLIVPLKVED
                     KIFGVLELASFNEFEDYQIEFVEKVAQSIGQTLQSVRTNIRTAELLAKTQQQAEEMKA
                     QEEEVRQNLEELETIKEELEKRTAEMEENQKQLEWEKSLLDSLLNYLPDKIYFKDLKS
                     RFIKVSKSTLEFFGMSKQEELIGKSDFDFFTEEHARPAYEDEQRIIRTDTPIIGIVEK
                     EVMNDGRVTWAETSKLPLKNSHGETIGTFGITRDITASKMMEEEISKKSEKEEAFQRD
                     LEKKVIELENLYKAINDSAYVIEYSPEGNVTFINEAYLQLLNLNADDVIGKHHSYQME
                     FTDEQRKNYKQFWDDLNNGIVRKETSKFSINDKTYLFYETYTPIRDAEGNVVKIMKIA
                     FNVSHLLSENQI"
     gene            32232..33545
                     /locus_tag="PK049_02565"
     CDS             32232..33545
                     /locus_tag="PK049_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HOK60470.1"
                     /translation="MLSWKKLLSPIRTGESPSKSTIDYTRTNFQRDYDRLIFSSPFRR
                     LQNKTQVFPLPGSVFVHNRLTHSLEVSSVGRSLGRSILNLLSPERFECPAQLITDIPT
                     IVSAACLAHDLGNPPFGHSGEDAFRHFFSNHHIPELDSWEASDLLRFEGNANALRLLT
                     HQFRGRRTGGFRLNYATLASIVKYPYPSTLKEKKFGYFKSEADVFNEIASQTGLIQIS
                     NGRYCRHPLVYLVEAADDICYQVMDIEDAHKLGILSTSTVTNLYLSFFDDETTSKKKD
                     IHETLSSVNDINERIAYLRAITISKLIEQCSSNFSKSYDQIMSGQPVKPLIDQMEGTS
                     AKAMDEIRKVSVNQVYNHSKVVEIEIAGFTIIGELLDIFTSAVLEPTKPISKKALRLI
                     PEQYLAESSDNYSKVMAVVDYISGMTDIYALETYQLFKGIKLPSL"
     gene            complement(33548..34510)
                     /locus_tag="PK049_02570"
     CDS             complement(33548..34510)
                     /locus_tag="PK049_02570"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOK60471.1"
                     /translation="MLNQIKEPISNHLNDFEPFFRKNMGSDIKLLDLITNYIVRRKGK
                     QLRPMLVFLSAGACGQINQSTYVAAGMIELLHTATLVHDDVVDEAYERRGFFSINALW
                     RSKVAVLVGDFILSRGLLVAIENGQVEMLRVISSAVKEMSEGELLQIERSRKMNIDEE
                     TYYDIIGKKTASLIASCCANGAISANATKDMVDRIYQFGKYLGMAFQIRDDLFDYQPS
                     GLIGKPTGNDIKEHKLTLPLIYALSQSDTHERRRIIRLINSGKKANIAEIVDFAKDKG
                     GIDYASKKMFEFRDNAKQTLEIFPDSEYKKSLLLLADYMVSREK"
     gene            34669..34968
                     /locus_tag="PK049_02575"
     CDS             34669..34968
                     /locus_tag="PK049_02575"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOK60472.1"
                     /translation="MVQVEELKIEQLEQAASMLKAIAHPLRIGIISLLEKSGQLTVTQ
                     IYETLGIEQSTASHHLGILKDRGVLSCKREGKNTVYFLRNENLGKILDCVGRCTV"
     gene            34979..35488
                     /locus_tag="PK049_02580"
     CDS             34979..35488
                     /locus_tag="PK049_02580"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOK60473.1"
                     /translation="MTTFDIIIVILLLVGAVRGFISGLIMQLTSLAALLLGIWVAIRF
                     SDFTAMLLMEKAGLTGQYIPLVSFAITFVVVVIAVHFTGKLIDKFFNLTPLGIVNKIL
                     GLVFGVLKYALIISVIIVFVEKANQRFNFYSEQTKNKSMLFKPLSKLAPSIFPYLHFE
                     SISERLKAK"
     gene            35593..36888
                     /gene="tyrS"
                     /locus_tag="PK049_02585"
     CDS             35593..36888
                     /gene="tyrS"
                     /locus_tag="PK049_02585"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOK60474.1"
                     /translation="MNFVEELKWRGMVHDMMPGTEEQLTKEMTAGYIGFDPTAESLHV
                     GSFAQILLLKRFQLAGHKPIALVGGATGMIGDPSGKSQERNLLDEETLQRNLEGIKRQ
                     LSKFLDFESGKPNSAVLVNNYDWMKGYDFLSFIRDVGKHITVNYMMSKDSVKKRLGEE
                     SKEGMSFTEFSYQLVQGYDFLWLYQNMNCKLQMGGSDQWGNITTGTELIRRKLSGEAF
                     ALTIPLITKADGTKFGKTESGNVWLDARLTSPYKFYQFWLNTSDEDAERYIKIFTLLD
                     KTTIDSLIAEHKKEPHLRKLQKTLAREVTVMVHSEDDYNKAVEASEMLFGNATTDMLN
                     RMDEDTFLSVFEGVPTFEVKKSELAKGIPFSVLAAEITKVFPSKGELRRLVQGGGLSI
                     NKQKVDNPDITIGTETLLNGKYILVQKGKKNYSIILAVD"
     gene            37006..38010
                     /locus_tag="PK049_02590"
     CDS             37006..38010
                     /locus_tag="PK049_02590"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOK60475.1"
                     /translation="MDNNDISMDKSMLSIAQTIWNGRKTIITVGIIAIIISTFASLLV
                     KNQYKATATIFPPVANQVSKELITENKQEGLTTFGETEEMEQFLQVLSSRTLKDKVIS
                     RLNLQQHWGIDSTSKSYRFRSYQKFDEQVKMKPTRYQSINIEVMDRDPEWAAKIANTV
                     VEYSDSLMRAIKAQVAVNALMALEKQFALVEHDLNNLQDSLALAISNGVVDPKRQATI
                     FYKEYINALLKANNAHLIKLKSEMYRFSDHGAKSIRYAAEIKDLAERLNELRKSIVVA
                     RIEANQEIPTQFIIDKADIPDKKAYPKRSIIVVTSTLAAIIFAIFLLFLQEYLKRLKI
                     "
     gene            38014..39543
                     /locus_tag="PK049_02595"
     CDS             38014..39543
                     /locus_tag="PK049_02595"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOK60476.1"
                     /translation="MIPFAGSVYPLMDIIKSILSHIKNKWIIVSSIIFILINAILIGT
                     ERDFFVILPIIFIFLLAYLSFPLKFYLAYLFISPLSIPLSEFFQEISIDLWLPSELML
                     VALAPIALIMLLSNNHQNRWFTWHPVLLTIYFFLGWIALTSITSSMPLVSLKYLILKT
                     LYFIVLLYLPFILLVKGNITFKRFIAFYSIGLFVVILLTLSKQWEYGLFNKFVAHGSC
                     NPFFTDHTSYAAVLALLIPVSIALLISSNTATRRILFLALTIVLIVAFVLSYTRAAWL
                     SLMVTGVVWVAWLLKIRFRYLALLTIIFFGSIFYFQDNILIWLNQHKTASSGDIRKHI
                     SSIANIRTDESNVERLNRWTSAIRMFEEKPLLGWGPGTYMFQYAPYQASYLKTRESSN
                     LGLKGNAHSEYLGLLSEAGLPAALAFILIIGILFYKGFNLALKLKPKSADRVILIGVL
                     HGLITYDVHGFMNNFLDMDKVAVLFWGYAALILAMEHLYSKSNPRIEISSKTSASDSL
                     S"
     gene            complement(39477..>40297)
                     /locus_tag="PK049_02600"
     CDS             complement(39477..>40297)
                     /locus_tag="PK049_02600"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOK60477.1"
                     /translation="TIRHLNFVQTLSIIIFVAIENLRLFHKSLEQEAMRKELELASRM
                     QSMLIPSPADMPKFQGVSIGSYYSPHFEVGGDYYDVIALSPNDMGFCIADVSGKGISA
                     ALLMSNFQANLRALFSKDISLSALVEKLNERVMATAKGEKFITMFIGRYNNQTRKLEY
                     VNAGHNPPFMFVRNSSKIIQLSSGCVGLGMIDDIPIINQGVLNIDEPSTLLCYTDGLV
                     ETIDGDKVVYNTRILEKNFSRNHSVQQIINTIAQERESDADVFDDISILGFDFE"
     assembly_gap    40298..40397
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<40398..40855)
                     /locus_tag="PK049_02605"
     CDS             complement(<40398..40855)
                     /locus_tag="PK049_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60478.1"
                     /translation="MSITASQNRLKLSTFKLNALLGMAQAISAELKTEELIDRFKRIL
                     NDDLGIDRILLYKIEEGKWQLLLNSNCPERVVENINVERDLLHFNEITFVGVSENPIL
                     LELDVIIPVIQNNQPVGYVLIGDTNEYEKGVSATIRHLNFVQTLSIIIFVA"
     gene            complement(40953..41519)
                     /gene="efp"
                     /locus_tag="PK049_02610"
     CDS             complement(40953..41519)
                     /gene="efp"
                     /locus_tag="PK049_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOK60479.1"
                     /translation="MATTADFKNGLFILFNGKIYTIVQFQHVKPGKGGAFVRTKLKSL
                     ENGKVIENTFNAGVKIDVVRVERRPYQYLYKDELGYHFMHQETFEQVSLDEYKIENPD
                     LLKEGQYVEMMVEADNENILTCELPPFVEMEVTYTEPGLKGDTASSTALKPATVETGA
                     VINVPLFINIGDKIKIDTRTREYSERVK"
     gene            complement(41645..>42112)
                     /locus_tag="PK049_02615"
     CDS             complement(41645..>42112)
                     /locus_tag="PK049_02615"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOK60480.1"
                     /translation="YRHYSTQTWDHWSRPDSSIVISSKTGKVVVVKGCQDILSSVYYL
                     RNKMLTMPPLKPDQTITVETYFTDEKYPLTVRFKGYETIKTKFGMVKCMKFMPVVITG
                     RVFKTKDDLTIWLTNDSNFIPIRIKFEIFVGSVYCDLIEYKNLANEFTALENK"
     assembly_gap    42113..42212
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<42213..42599)
                     /gene="tsaB"
                     /locus_tag="PK049_02620"
     CDS             complement(<42213..42599)
                     /gene="tsaB"
                     /locus_tag="PK049_02620"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOK60481.1"
                     /translation="MAKILCVETGTSVCSVAIGDENGILGVRELSDPKAHSTMLPRLI
                     NDVLTDASLTPSQISVVAVSKGPGSYTGLRIGVSMAKGICYTSGIPLIGVCSLQAMAV
                     GLSLSHPNIPADAILCPMIDARRMEVY"
     gene            complement(42613..43917)
                     /locus_tag="PK049_02625"
     CDS             complement(42613..43917)
                     /locus_tag="PK049_02625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOK60482.1"
                     /translation="MKKGLIKYLLGAVVILIVFAVVAKKKGWIGKPLTTPVYVEKPEY
                     RTITEAITANGKIQPEVEVKISSEVSGEIIELPVKEGNWVEKGTLLCRIKPDTYISLK
                     ERAIAAVNSANARLIQAKAQLAQSELSFNRSKQLYEQKAISESEFETARTNYEVAQAE
                     LKAANYSVESAQASLREAEENLRKTTIYAPISGTVSKLNVELGERVVGTIQMAGTEIM
                     RIANLNRMEARVNVNENDIVKVKIADTALIEVDAYLGRKFKGIVTQIANTANVQGITT
                     DQVTNFEVRIFILEKSYKDLLTNSQTSPFRPGMSTTVEILTNTKENVLTIPILAVTTR
                     IDTVKKTGEWEKVENNTQPVESKPTKVEKPKEIVFITKGDTARIVEVKTGIQDNQYIE
                     IIEGLKGDEEVITAPYSAISRKLKDKSLIRKVKNKDELFKTE"
     gene            complement(44008..45348)
                     /locus_tag="PK049_02630"
     CDS             complement(44008..45348)
                     /locus_tag="PK049_02630"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOK60483.1"
                     /translation="MKKTILPIFILLILTSVVFSQEPWTLERCIEYALANNIQVKQQE
                     LNVKVNEVNLLQSKMDALPTININGNHSYSFGLVTNYLTNTKEALNTQATSFSVSTGL
                     PIYNGFQLTNTRKQNHFDLKASVADVEKVKNNIALTIASLYLQILYQQELVEIAKRQV
                     ELSTMQLERTKVLVSAGSLPEGNLYEVEALVASDELQLVNAQNQLNLSYLNLTQLLEL
                     KTTEGFQIAKPVIHQLDSVKLVSESPSQIFGVAEGIMPQIQSARYRLQSSELGLKIAK
                     GGLQPRLSLGANYNSGAQYRIQDNYAFDGDPFMTQIKNNASKSVGLSLSIPIFNGLSA
                     RNRVTTAKIGLMNAQLALESERNALYKDIQQAYADAIAAEKKLQSSQKSKTASEESFR
                     YTQNRFNLGLVTALDFTSAKNKLAQAETDLLQAKYELIFKTKILEFYKGLPLKL"
     gene            complement(45517..46266)
                     /locus_tag="PK049_02635"
     CDS             complement(45517..46266)
                     /locus_tag="PK049_02635"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOK60484.1"
                     /translation="MIILVISDLHIDTGKKLGTFGWKAKAFMSTLDRVIEHYGVEKVI
                     LNGDIFDLYKYSYAEIAEVNAKIIDYFNRRGFIQLRGNHDIWVPHTHDHFHIKNSAGK
                     TIHIEHGHKADFLNGTRFGRFISVTMYSFLKWLVRFRWVERKFYSAVDYFDDVNRIPR
                     KYNTIKYLMYALKLLKKYDMVILGHTHKLESHKVYYLNSKKIYLNCGTCSLGRFQAVV
                     VDTESFDFETIKIPRKTEFDESQLPIFKRKR"
     gene            complement(46301..46828)
                     /locus_tag="PK049_02640"
     CDS             complement(46301..46828)
                     /locus_tag="PK049_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60485.1"
                     /translation="MKRFFSYYAVVAFLIYVLAILVVKLIINGYQEFSVSFSGILIES
                     AMAGFVFVFLFFGALWLNLSKTLGYIESDSFEEPAYGHRLSEQVETPIREFSKLKERF
                     TSNYSYVKVSDDRAAIKFHNGLTWKNWGTGGIAYLNTENGNVRIVLVPFMGYSMHATK
                     QLENEMKRVKEVLMG"
     gene            47146..47658
                     /locus_tag="PK049_02645"
     CDS             47146..47658
                     /locus_tag="PK049_02645"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HOK60486.1"
                     /translation="MKESKDLVRWYAVYTKPRNEKKVYARLVEKGIETFLPLQKKLKQ
                     WSDRKKLVDEPLFRSYIFVHIDLKRYYDVLNTQGVVRYITFEGKAVPIPDRQIDQIKQ
                     LLVQDIELEAVEGQIEPGTRVEVRFGSLQGIEGEMVEHSGKKKVVIRIDHISHSLLVT
                     LPAEYVVVKR"
     gene            complement(47700..48407)
                     /gene="pepE"
                     /locus_tag="PK049_02650"
     CDS             complement(47700..48407)
                     /gene="pepE"
                     /locus_tag="PK049_02650"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HOK60487.1"
                     /translation="MVRLLLISNSTNAGEAYLEHPKKEIEKFLGSIKRVLFIPWAAVT
                     FSYNEYEQKVQERFSEFGVEVDSIHHHRNAKLAVQEAEAIVVGGGNTFKLLKTIQQTD
                     IIEAVRNKVLSGTPYIGWSAGANVACPTICTTNDMPITEPDSFSAFSLVRFQINPHYL
                     DANPEGHAGETREQRIMEFVEMDPHVYVVGLREGTMIKLEDVQFSLIGPKPARIFKKG
                     QPPREVYPGDDLDFLFE"
     gene            complement(48458..50389)
                     /locus_tag="PK049_02655"
     CDS             complement(48458..50389)
                     /locus_tag="PK049_02655"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOK60488.1"
                     /translation="MLRISIISAILTFAILFNNGLAQNHSIDSLNQALQKQESDLNRY
                     KTLTELAIAYSDSNYEKSLNYWKSALNLANRLGKRELVANAYHQIGFSYMKMGEFRLS
                     LENLENAGTIYKYLDSVRLLAGIQNDIGLIYRNWGKYDKALEFYLNALDLYKKIGDIE
                     GTGIASNSIGQIHFYRENYPKAIEFFKEYLEINEAKGNSRAVAGASNNIASAYLELGK
                     YDDALEYFLKSLHIYDSLGISIGVAIIQDNIGSLYFKQNLLDNALLYHQNALEMFQSL
                     KSVTRVCYTQKNMGQVLLAQGKVERAINMFQESLNKAKQIGLKDVESDCNKFLSECYV
                     KTNNFQKAYSYLLDHITIKDSILNSETLQKIEELQAQYERDKKEQEINAMNSKLKAQR
                     LNLFIAMGFSVVFISLFLLVVIENRKKKRALVNLELLRNHLFQNISQNLCNLQMVKNS
                     YEVEGYFSAWWEAKIKKVDEKPNFFHFLYKNYTFCYYVIAAEPNACCDFINFNIYNIV
                     IEYIKNNGELNEGVLEPLNLYLKDDPIVKSLGKNSIKLYPFILYKKQILTLCPDNMAF
                     RQFGSFIVSSTLNWSNLRPGDIVYLFGTSDDKSAMNELRKVVKSIDLMEFAEQKDLAL
                     NFIKSLELKEDAFIAAFKV"
BASE COUNT        14678 a        10803 c        10058 g        14999 t
ORIGIN      
        1 ccatacgcac caaccaaatg cttatggaaa ttgataggtt tttggtgaac taataatggt
       61 ggggctgaaa agactaaagt aatagggtaa gatattatac agctaccatt catttttaca
      121 gatttattca aataagaatt tataagaaaa aaattccatt tgtcgcttaa ttctacctaa
      181 atttgcttca acactcaatg aaactaaagt aaaaactccc ctcacaatgg acattcttat
      241 tatcataatc ggacttttca tggctgccct tggttttctg gctaaagcct accctaacct
      301 gattgcgggc tataacacca tgcctaggga taaaaagaaa aatgtcgaca ttgagggttt
      361 gtcaactctc atacgcaacg gccttgccat tacagggctt accattattg tcagctactt
      421 tgtttttaaa tggctaggct ttaccactgt tgctaacctg atgatagtta ttgtaaccct
      481 tgcaggagtt actactattg ccgttaaggc gcagcgcttt gaccataaca aaagcaaatc
      541 aaaacttcac atcctggttc ttggtgcagc aatcatcttt gtagcgagct tgctcaccta
      601 tggtttcatt cccacaaagg taatttatgg caataattcc gttaggttta gcggtatgta
      661 tggcattaca gtaaatatcg atgaagttgc aagcgttgaa cttgctaatg ccatcccaaa
      721 aataaagcta cgcaccaacg gctttagcgt tggaacagtg aataaaggca cttttattgt
      781 tgacagtatt ggcaaatgcc gactgctgct taactcgggc agtaagcctt acatagttct
      841 cacaaaaacc agtggcgagg tttgtataat caacctgaaa agcagctccc aaacccacga
      901 tgcctaccaa aacattttaa aacttgtatc gcaggggaaa aagtaattat ccgaggtact
      961 aacctctgca aagctttatc ttttccaggt aagcaggtta tggcgcatac caattgattc
     1021 acaaaaaccc gtttacaaca tccgttagca caaaattctt ggcttgggtt taagtgatat
     1081 gccccctatg ggtgtatatc atgaaacaaa ccaactaaag cctatgatac gatatatttg
     1141 ttctttaatg ttgatgttaa ccatatcggc aggtctttat gcacagcgta ccctaacagg
     1201 tattgttgtt gatgctgaga atggtgaacc cctgccaggg gcaaccgttg tagttgatgg
     1261 aacaacccgg ggcacatcaa ccgattttga gggaaagttt tccattgagc ttggtgaaaa
     1321 cgagatgttc ctgatggtaa gctttgtggg ttacgacaaa aagcgggtga atgtgggtaa
     1381 ccaagcaaca attcgcattg agctcaacag ggaatcgacc gtactgcagg atgtaattgt
     1441 aaccgcaata gggataaagg ccgagaaaaa ggcgttgggc tacgccgcgc aggatgtaaa
     1501 gggcgatgag ctcagcacca ccaaccagtc gaatattgta aacgcgctta gctcaaaaat
     1561 tgccggtata agcgtaacca gctcatcggg aacacctggt gcatcggccg atattgtaat
     1621 acgtggtcgc tcgtcgctgc gcgccgatgg caactcaccg ctgtttgtgg ttgatggtgt
     1681 acccattgac aacgattatg ccggaagcgc cgtttacgat tactccaacc gcgccatcga
     1741 cctaaactcc gacgatatcg aatcggtttc ggttctgaag ggtgcagcag catcggcact
     1801 ctacggtatt cgggctgcca atggagcaat aatcatcacc accaaatcgg gtaaaggaaa
     1861 gggtatccgc aggaatatca cctttaaatc aacactgggt ttcgataggg ttaacaagct
     1921 ccccgaaaaa cagtaccgtt acgcacaggg tacaaatggc agctactcca gcacaaccaa
     1981 tttcagctgg ggcccgctga ttgacaccct ccgctacgat ggcgatatca actacccgta
     2041 cgataaaaac ggacgaattg taggcatgag cgacccatca gcaaccgata agagggtaat
     2101 tccctacaaa aacatcgacg atttctttga aactgccgta aacagcaaca cctaccttag
     2161 cataagcgga ggatccgata atggtagcta cctgttctca atgggttacc tcaaccagaa
     2221 tggtgttgta cccctaacca gctttgaacg gttaaacttt aagctctcgg gcgatacaaa
     2281 gctatcggac aagctaaaaa tttccggttc agccacctac tccaattcac agggtagcta
     2341 tgcgcaaaag gggagtaacc tatcggcagt tatggttggt cttatgcgat gcacgccaac
     2401 ctttgatctc actaacggct ccagtgatcc cgttaacgac cccagtgcat atatgttccc
     2461 cgatggaacc caacgcaatt actactcaaa gtacgataac ccttactggt cagtaaataa
     2521 gaaccgctcc agcgatgagg taaaccgtat tattggcaac agccagattg actaccagat
     2581 tttaccatgg cttagcgcca tgtacagggc gggtatcgac ttttactcgg aacagcgcaa
     2641 ctcattcttc gacaataact ccaacgacgt taccaacggc tacgtaaccg ccagcctgta
     2701 taatttcaac agctttaact ccgactttat acttacagcc gaaaaggata tcacaaacga
     2761 tctcaagcta aatgttctgc ttggccataa ctactacaac aagtacacct acctgaactc
     2821 gcagcgcggc gattcgctta ttcttccaaa cttttacgac ctttcgaaca ccgctgtggt
     2881 tactggcgac gattacatct cccggtacag gattgttggc gcatactacg atatcaggtt
     2941 gtcgtacaaa aactacctgt acctaaatac tactggtcga aacgactggt cgtcgacctt
     3001 ggctaaggga aaaaactcgt tcttttaccc cgctgttaat ggtagcttca tcttcaccga
     3061 ggcatttaac ctgaaaaata aaatttttag cttcggtaag gttcgggcat cgtgggcaca
     3121 ggttggtaat gatgctccta tctactcatt acagaactac tactcggcca taagcggcgg
     3181 tataaacgga caaacctcct ttgccaccca gcgaaccatt gggaataaca acctcaaacc
     3241 cgaagagacc cgttcgtggg agattggtgt tgacctgaaa tttttggata atcgcctggg
     3301 gttagatatg gcctactaca gctctacaac cgacgggcaa atagttgaag tgccagtgcc
     3361 ctactccagc ggttacgacc gtatggtgct aaatgctggt ataatcacta acagaggtgt
     3421 tgaggcacaa ctttacgcaa ctccgcttaa gatggacaag tttacctggg atatgcaggt
     3481 taactttacc cgtaatagga acatagttga ggatttacct gaaggtattc ctttactcga
     3541 gttccagaat accggattga gtagcacccg gtctgttgcc attaagggtg aaccctatgg
     3601 ggttctgtat ggaacccgtt acctccggaa cgacaagggt caaatcctgg ttagcaacga
     3661 tggttatccg ctggttgacc ccgttccggg tgtggttggg gatccaaacc ctgattttac
     3721 aatgggcatc cgaaatactt tcacctacaa ggaaattacc ctcaccgctc ttgtcgacat
     3781 taaaaaggga ggcgatgtgt ataatggcac caaaaatgta atgagctacc ttggcaccca
     3841 caaggaaacc gagaataggg atgaacttta cgttttcccc ggtgtaaatg taaataccgg
     3901 cgagcccaac acggtggcag taaagcgtaa ttttgattac tatagtcgtc ttggaacaat
     3961 ggcaggactt tccgaagctg gcgttgaaga tggttcgtac atacgtttac gtgaaattgc
     4021 cattaactac agcttgccca ctaaatggtt gaacagaacc cccattaaag gtttaatggt
     4081 gggcattagc ggacgaaatc tactgctttt cacaaaatac tccggcattg accccgaaac
     4141 aaacctttcg ggtacatcaa acagccttgg tcgcgactac tttaacatgc caaacaccaa
     4201 gggagtagag tttaacattc aggttaactt ttaataccca agcgctatga aaaaggtaaa
     4261 taaaacaata aaaatcactg caattgctac actattgcta atggtatcgt gcgagattag
     4321 cgaagtgaat atcaatccca atagtccaac acaagtaccc gagaacgtaa tactaccatt
     4381 tgccgaggaa agcattgccc ggttaatggc tggctcaggc caggtaatgt caggtatatt
     4441 tgtgcagtac tacgagggta tcgataacca cccccgacag gtgcagcagt atgttgttaa
     4501 cgaggctctt tacgttgaat gggactggaa cgactactac gatgggccca tgattaatct
     4561 gaagaaaatg attgaagtag ccaagggtaa cggcaacttc tactacgttg gcattgggaa
     4621 taccctatta gccctttgcc taggaaatgt tacatcgctt tggggcgata ttccttactc
     4681 cgaagccctt gatggcgcta ataataaatc gcccaaattc gatactcaaa aaagcattta
     4741 tgaagccatt caaacactgc tcgatacagc gattgtaaac ttcaataaat cgtacaacgg
     4801 tcgtaaaccc acaaccgacg acatactatt tggtggtgat attgccaaat ggaaaaaagc
     4861 agcatacgcc cttaaagcaa ggtattacat gcaccttacc aagcgagcag ccgaactcga
     4921 ttttaaccca gcacagaaag cgcttgaggc ttgccaaaat gccatatcat caagcagcga
     4981 cgatatgctg ttcccttatg gctactccgc ctcagagtat aatccattct actcatttac
     5041 tcgtttaggc tatataatac caaacatttc atttacttcg cttttaagcg ccctgaacga
     5101 cccccgcaag gatttctact acaagaaaaa gtttggattg gcaaaccttg aaaacagcta
     5161 ctttacttcg cccaactcgc cggtttttat gattacctac cacgaactta agttcattga
     5221 agctgaagcc cgactcaggc tcgatgaaaa cgacccattg gctcaaactg ccctacagca
     5281 aggtgtaaga gcaaacctca acaagatttg cggcgatgcg gtgccacagg caaccattga
     5341 tagctacgtt actgcaaatg caaaccttac aggcgatttt gaaaataagc tgaaaaccat
     5401 aatcaggcag aagtacattg ccatgtttgc ctccattgaa agctggaccg actaccgcag
     5461 aactggctat ccagttctta ctccaaaccc aaatggcgat aatccacaaa atccgggtgg
     5521 agcaattcca cggaggttag cataccccca aaccgaacgg ctttacaata aaaatttccc
     5581 tactcctctt cctaccctgc aggatagatt ctggtgggac gagtagattt ttttcattgg
     5641 cgttatgttt gttaagaacg gctcctatgg gagccgtttt tttagtaacc cggattttgg
     5701 tcggctgggg taatttgagg attataaaga atttctgtct ggggtattgg gaaaagcgtt
     5761 tggtttactg aggtaatact gggtattttt acaagagccc tgttggttct aaccaaatcg
     5821 aaccaacgct tgccctcaaa ggcaaattct agctgttttt cattctccag gataggccaa
     5881 agctttccta gtgcagtggc cgaatcgata gggttaagac ctgctcgttg tcgaattgta
     5941 tttatatcgt taagtatggt attgaagtgg gctacagaat cggtaaagta aagcgcttcg
     6001 gctctgatga agtaaagctc agcattgcgt aaaacggtaa cgttgtttgc tccggttgaa
     6061 aggtttgggt atttttttgt ataggaaatg atcccagagc ttgttgaaag cgactcggca
     6121 atccacctct tacgtttatc gcttggtgct attaagctta ccaaatccga tttaggagca
     6181 ttctcgtatc ttccacctag ctggtttggg aaatggtatt ctgccaaacg gttattatcg
     6241 ctcggtatga atggtatgta gaaaactatt tcggcactag gctccgtata tgaaccgaat
     6301 agcttgctgt aatccgattc caggtatgag ttttccaaca aacattcatt agcatactgc
     6361 aaggcatgtg gataatcggc tagagttaaa tagaccagag cttttagcgc tcttgacgaa
     6421 cgaaccgttg cccactgagg ctgaatattt tgaattgata tttcagcaga atcaaggtct
     6481 gaaataattt gctgataaac actaccaatg ggtgatcgtg gaagaaaatt tgagggatcg
     6541 tttttggttg tgggtttaag cttaagcggc acagcaccat acaggcgaac aaggttaaag
     6601 tagtgcagag cccttaaaaa tagaagctga cctacgatgt cgttcttttc cgtttcgttt
     6661 aaaaaattta cgtcttgaat tccttcaagg atgtagtttg ccctattaat tgcagtgtaa
     6721 atatcctccc aaaccccttc aacaataata ttatcggggg tcaaattatt ctcatcaagg
     6781 gcaataagct caatttttgt tccctttgcc acgctattgt ccgagagcaa atcgcctgca
     6841 atgataaaac tacgaccgaa gtaatgctga agctggaggg catcgtagca accggtaagg
     6901 gctgcataaa ggttttcgcg ggttgtaaaa acctcatcgg aggcaacata gttcttcggc
     6961 ttaatatcga gccaatcgtc gcaggctata aacggtatga gcaatatgga tattagaatt
     7021 ttgcgcatac tcccagtatt aaagttttag gttgtggaca ggtaaagaaa tcggtgccca
     7081 tcactataga gttttcaccg ttgtagttca cttccgggtc cataccgctg tagcgggtcc
     7141 atgtaaacag gttcttcccg gcaatgtaaa actgcaaacc agcaaatgca gttttcctaa
     7201 tcactttttg gttaagtgta tatgttaact taatgctctt caaccttaca aatgaaccat
     7261 cctcaacaaa cctcgatgaa atgagtttgt tatacgttga ttctttaggt atatcggtaa
     7321 tatcgccggg ttgcctccaa cgccgaagta ctgctgtggt ttgattggat tttgatagcg
     7381 tttcggtgta cattcgggtt gcattgaaaa tctcattacc tatggaataa aaaatgttaa
     7441 agttaatgct aaacgaacgg taagtaagtt gacttgtaag agcgccaaac aggtcaggga
     7501 atggggatcc aatccgtgtt ttatcaaaat cgttgattat accatcgccg ttcaaatctt
     7561 tgtaaatcat cattccatcg gccggattca caccaagagc tttgtagccg taaaaaaacg
     7621 ataggggttg tccctcttca atactactgg aggctcgccc aatattcctt ataggttggt
     7681 tttggtatag gcttactact tcgttcctgt tcatggttat tgtaaatcct acgttccagg
     7741 aaactagctc agtgagcaaa tcggcattta aatcaagctc aaaaccgcta tttcgcattt
     7801 ttcctatatt ggaaatgata tggctgaacc cggtagaggt agggtaaggc aaacggtaaa
     7861 gcaaatcgaa tgtgtttttt acatagtaat cggctgaaag acgaacccta tccaaccagt
     7921 cgacccgaat accagcatta aactgatggg tggactccca ttttaaattt gggttagcaa
     7981 tttgagcagg ttgtaatcct gctgagccgc catagtttga tcctggggcg aataggtcga
     8041 gtgcccggaa gtcgctgatt tggtcgtttc ccgtaaggcc ataactcaac gttaacttaa
     8101 ctcttgggaa tagggtgtgt ttggggtaaa agggttcgtc atatgcattc caaaggaatg
     8161 caatagccgg gaaattccca aaacggttac tctcgccaaa cttggatgat ccatcgcgcc
     8221 ggaagttaaa tgtaaacata tacctgttca ggtacagaaa tttcaaacgt gaaaataccg
     8281 aaaggttcag cgcatccact tcccgtgagc tggcctgaac tggtattgcg cctccctgta
     8341 aaaactctat gctattacct gggaaacttt gcgaccttaa gtaactacca tgctccatgt
     8401 atcgctcagt gcttaaacca cccaaaacct caaggttccc ggttttccat ttcttggtaa
     8461 aattagcata cgatgaatag taaaacgtaa taacattgct ggttgcctca attccaagtc
     8521 catcgtactt tgctccttgt cgggtttgtt ttggagcaaa ggtttgttca ttcaggttat
     8581 agtaatcaat acccccttga gcgtttattt gaaaattgct gcttggttta tacgttaatg
     8641 tcaggtttga aatattccta tgcgatttgg caaggttttt ttcctccttt gctatcgaaa
     8701 tggggtttgc ataggggcca tcgttattga atgtaccatc gggattataa acggggtata
     8761 tgggtggcat tgatatggcg ttgggcaagg gcccattaag cgattggtcg ccctcaaccc
     8821 tattgttagt tgaaaagctg aaaccattac ccacctgcag ctttaacttt gggtttatgc
     8881 tatagtcgta gttaaccctg aacccgtagc gactaaagtc gctacctaaa acaattccat
     8941 cctggttata gtagttaccg ctcacgtagt atgacgagtt gctatcgcct cccctgaacg
     9001 atagctgaag gctgtatgtt ggagcaaggc gaaatacttc atcgagccat cgggtatcaa
     9061 ccttattgtt ttgtataaaa tcatcggaat aaaccggggg ctcgcctcgt tgaatggctt
     9121 cctcgttaat catattcatc cactgggtgg catttagcat gttgagcttc cggttggcct
     9181 gttgaagccc aaagtaggaa tcaactgata tgctgttggc gcttgcttgc cccctttttg
     9241 tagcaaccag cactacccca tttgaagctc gggtgccata tagcgcgctc gatgaggcat
     9301 cttttaaaac agtaatcgat tcaatgtcgt taaccggaat atcggaaatg gcatcaatgg
     9361 tttgcccgct aaaatcgagc tgcgagtagt ttccagtaat tacgggaacc ccatcaatca
     9421 caaaaagagg cgagctacct gctgaaatgg atgtgattcc ccgaatattc atttttaccg
     9481 atgagccggg tgttcctgca ttgttttgaa ttagtactcc agttagattt gtttggaaag
     9541 cacccattac cgaggcatcg tttacctgtg aaaaacgttg gggatcaact accgatactg
     9601 accccgttaa aaggttacgt tgttcacttc caaaagccac aaccacaatt tctccaattg
     9661 ggtttaacat tggttttaac catagcttta caacggtatc ggtggttacc tggtaattca
     9721 aaggatggta tcctatacac gaaaagttta acgtttgtcc atacttaacg gctagggaga
     9781 attgacctgc cgaatcggta taggttcctt tcatgtcgct ggtaataaca tttgctccag
     9841 caataaaatc gcctgtggaa aaatctacaa ctgttcccct tagcgtaaat gggggcatta
     9901 gccgtaaagg ttttagtaca actgtattgt taattatttc aacactacac tcaatggttt
     9961 tcccaagcaa attaaataca tcggctgctg cttttttgcg tgccgagaaa gtaactattg
    10021 tatcgacatt taagtccgat gaagagaatg caaacttata accggtttga gatgaaatat
    10081 cctttaaaat gtcgcccagc ttctggtctt taaatgaaaa actatatacc gctacatcct
    10141 gagcgcaaag tgtgtaaaaa aataacaaaa ttgctaaagt cgatagaatt ggcctcatgc
    10201 tacaggatta gtttaccgaa aaaattccat gaaatgtatc aaaaaaaaag aatctaaaca
    10261 acactaaaca aaaaaaccgc cccaatggag gcggtgtaca atccattatc caaaacattt
    10321 atttcccaaa atagtacaat gcaaaaacta ataaaatcat ggaaacccca aaaagaatca
    10381 gaagtaacct tataaaccat ttaacccatg tagtataggg gattttggca attgaaagga
    10441 ctcccatagt aacgccgcta gttgggacca acatgctgaa tagtccatcg ccaaggtgaa
    10501 aagcggtaac tgcggtttga cgtcccactt caagtaaatc gctcagggga gctacaattg
    10561 gaacggtaag tgcagcctgt cctgaacctg atggaaccag gaaagtaaac aatgattgaa
    10621 aaatgaaaat aacctgcacg gaaagttcag gaggcaagct cttgcctaac ccagaaacgc
    10681 taaaaagtat agtgtcgatt atttttccct gtgatgcaat tacaagcaat cctctggaca
    10741 taccaattat gaggcctgcg gtaaccatgt ccttagcgcc attaatgaaa gcatcaaccg
    10801 catcgcgggt ttttaatctg taaactactg ctgaaagtac acccaaagca ataaatagcg
    10861 ccgaaatctc attaataaac catttccatt ggtttgcccc ataaaccaaa aggattagcg
    10921 atatgaatag caaaccaata atgatttttt gccttatatc aaatattttt atcgatgagt
    10981 cttcagagat ctcaacactt cggtctctat caatttcata aaccgaactg atctgtatgt
    11041 ttcttttaat ccgttttgca tagcgcatta cgaagaaaat ggctattgat gttagcacag
    11101 tccatatcag taccctgaac tcccagccgc taaataaagg caactcagca attccgtgag
    11161 caactccaat gttaaacgga ttagtaattg ctgctgcaaa tccaacgcct gcagccatga
    11221 acgacatcga aatacccaca atagaatcgt agccaagagc aatagccaag ggtatagtta
    11281 tcattacaaa aacaagagtg gattcactca taccaaaggt tgccccggct agcgaaaaaa
    11341 ataccattac aatgggaact attatgctat tcagccgggg attatcctta cctcgctcca
    11401 aaagtctatt cagtgcagcc ggaatggcct tagtacgtgc tagcattcca aaagcacccc
    11461 caacaatcaa acaaaagcct attacttggg cggcctcaat aaatcccttt attggcgccg
    11521 taagcatcga tcctaaacct tgcggcgaac cgctaacctg atggtatgtt ccgggaacaa
    11581 ttacctttcg tccgtttact agctgcttct caaactcacc cgccggcaaa atccaggtta
    11641 acagaataag acctatcatt ataaaaatga tgatgacaat agtgtgggga aaaacaattt
    11701 ttttactcat tggtgaaggt ttagttcttt gacaatttta acagtttctg agtagttact
    11761 aggacgttcc tatcgtctct aaatcttaaa aagtaaatcc catcatgcaa atttgatatt
    11821 gaaattgttg atgtttcagt taacctggat tccattactt ttttaccggt tacatcaaaa
    11881 atttcaagta ttccggggtt caagacctca atactcacat aatcgatagc cggattggga
    11941 taaactttta tcaacatgcc taagtattgg ttttctgggg tatatgtagg cacattattc
    12001 cctagtataa ccgtatcggc agttcccaaa aagcgcaaca taacccgttc aaacccagca
    12061 atatttctcg attgactata aggcccctga ttagcaaatg cacctctagt ccctgtatta
    12121 ctgaaataca ccagcggaaa actgcccatt acgtttctta gaaacgattt ctgctgggag
    12181 tcgaaagtgt tacaggcaaa ggttgatcct gtgattaaaa ggccattctt cagcttatcg
    12241 cgtagcatta aatacgatac accggtaacg caattctcgt agtattggct attgataaat
    12301 gcattaggaa taatgcataa attttctagt aaccctatac cactgctgaa ctgaagttga
    12361 ccattatacg atgctcccga tgtggtgtag ttgtttacta ctgatgctcc tgcaaaacgg
    12421 gcattatcgc ccacaaagaa tgaaaccatt tcgggcgctt ttagcttggt tttaagcaac
    12481 aacccattac ccgctgcatt caggtagcta aaaagttcaa cacgatcgtt cgacacaaaa
    12541 acaaatttct ttgcggcatt tatcaatgcc tgatgctccg tattcgatga gttagcggta
    12601 aggccctgaa tgatatcgac tttagctgct ccttttgccg taaatgttga tttaagggag
    12661 tttgctctcg tctgatcggt tccggttact ataagaattg gatcggcact accgcctgct
    12721 tggttcacaa agtatgcgca agcgtcatcg ctaggataat cagtcccgct aaagaacaca
    12781 gtaattcgag aattaatttc gtttaccagc ggattgattg tgttgctgta gcctgacacc
    12841 tcaaaagtat tcaggttgat tgtggttcca tgaagcaatt gggttagttc aatgtagtcc
    12901 gattttagca ttgaaacctg ggtgtcgaac ggatttgtag cactattaac tctataaatt
    12961 cccacggcac cggttccgta aacagtagcc ataccgttag gcgaaatgca gattgcggta
    13021 tgatcatcta ttccaacacc cactgccaat tgcccggtct gcttatacca gctagccata
    13081 aaggccggta ttcggcctaa tcgcccccgc tcggcaacgt gagtatcaaa tataaaattc
    13141 ccttgaatgg tatttaaaaa gtcatttgca agggtaattt gggatgagta agcgctcatt
    13201 aaagcggttg atggatcaac cgatgctact tgtgcagtaa acacaatggg ggaaagtatc
    13261 attgctccag ctgaagtgcc agcaagtact cctccgctgc tgaaaacttg ctgtaatgcc
    13321 tgttgggttt ttgttccctt gtagtattcg taatatttac tttggtcgcc acctttcaaa
    13381 aaaatagcgc cataggtaat cagactatcg taaagccatt gggaattagc aagcaatttg
    13441 ctatcgatct taaaattttt tgcccactta gccccaaatg acttgaagta attgggtagc
    13501 caatcggttt cgtcgttaac gctaataatg gcaacctttt tgttagccga ctgggttacc
    13561 acccaggagt aaggtaaatc gctccaaccc ccgctacttt ctccaccgcc gcccgcaagc
    13621 atcaaaaaac cttgaccttt tgcataattc agggttacca acatcaaaaa aacaaatgat
    13681 aacgatttag taattgtttt catggttaaa atctatttga taactaaact atcgccgggc
    13741 aaaaaaacgc tcacctgaag attttcacca ccaaaaagcc cattgtcaac ttttgtaatg
    13801 gcttcaggat gcttaaccat aaacatttga cctttgccat aaaccttgat aacatttctt
    13861 tggatggtta tataagcaga atcgtcaaca ctgactatat aatccaatgg attttgcatt
    13921 gccaccgata acatttcgga aaatctatta ggcgaatttg actttactaa agcgattggt
    13981 aattccaaca agtcaaatcc atttgaaatt ataatatcat ttactcctac tttttcggtc
    14041 aataacactg aattgtttga tggatgagca atttttctga aaaatatttc gtcggcacaa
    14101 ccatcagcta taacaatttt tccttttact gatttttttt gtaataaatt ggaaatatat
    14161 aaaatgtcat tacgtttgct cgcatcaatt aaaagaaaaa ttaattgatt taaggaaaaa
    14221 tctgaattaa tagtttggtt tatactatat ttttttactt tccaattttt caagttaaga
    14281 ttactgtata atatttctgc tttttgtttt tttgaatcac tatatagaat tatgctatta
    14341 tctaagttga tattcttttg aggtatatca atattttgga attggtttga gcctaaatag
    14401 taaagagtta ttgaaccatt tttgggttca atgttttgac tacatgaaaa taaaagtagt
    14461 aggagtgata taaaccaaaa cctaggcatc aaaattactt tatagataat acaaataata
    14521 attgtgatac ccttaactac aacttttgaa taaatattat tggtctagtc cctccctaag
    14581 tgttttaagg gcaagtctaa tatgcgtttc tattgttttt tcggagatgt taagctgttc
    14641 cgctatttcc ttatggctta gtccttgttt tcggctaagg tcaaatactt ttttccgctg
    14701 aggcggtaaa tcgtttatta ttctttcaag ctgttcctga atatctcgga aaataatttc
    14761 attctccaat cggacgtgat ttgactcaag gtaggtgctc aaatgctcaa ggtaagctat
    14821 ctcatttacc cgcttacgat ggatattgaa aatggtgttt ctggctatgg tgaaaaggta
    14881 tgaacttaat gaaaggttta catcaatacg gtcgcgggtt tcccaaagct ttacaaacac
    14941 ttcctgtgtt acattttcgg catcctcttt ggaaatcata aagcggaggg caaagtagta
    15001 aatcctttcg gcatataaat cgtagagctg ggcaaaagca tcatggttgc cctcaataac
    15061 cttaagggcg atttgctttt ccctactcaa ctcaactgta acctcttggt tactcgtatg
    15121 ttcccttggc tcttgcaact ccctccctta acaaaatttt tcctttggct attatgtgaa
    15181 caatcgcaaa atcgttatta atacatacca aatcggcatc ggtgccaacc tgcaccttcc
    15241 ctttactttt caacttcagt attcgcgcaa catttgatgt taccacagtc aatgcctttt
    15301 caagtggtaa accctccttt aaatgggtat ctatcatttc gttataaata gatttgggtt
    15361 gacccatttc aagcctaacc agttcaccat ttttatcaaa taaagggagc gatccgtttg
    15421 catcggaggt catagtaatg tgctctaggg gaacacctgc cctcaccagt tccactattg
    15481 ctctggatgg cttaacctca tattcaggga agtaaggata agagctggct gttatatcaa
    15541 tatacccttc cttcccgtat ttttttgcat cctcaaatat gtaatcgtta cggttgcaat
    15601 gggttggatg gaattgcctg taactaagtt cgctttgcga aaccgcatcg tgaatgggtt
    15661 gaaaagggtt tttggcatcg cccatgtgaa tgttcacaat accagcttta cctccgagca
    15721 tacctcctac ccgggcctcc tcggccaacc tgattagctc tgcagttgta ggccacgaac
    15781 tgcgatggtc cgaaagggct atctcgcctg caccaataac ttcatcaata agtgccaaat
    15841 cacgtgcaat agagccagta atggttggtg tgggtacctg atacgatcct gtgtacatcc
    15901 aagccgatac cccttcagcc cttagcgatt ttgctttcat gagaacggcc tcaggggagc
    15961 gggttattcc atcggtaccc aggcagccta ttacggttgt aatccccccg gcaataagtt
    16021 ggctgagctg taactcaggc gttcgggtcg atgggccacc ttcgcctcct gctccagcga
    16081 tatgaacatg ggcatcaatt aaaccgggta tcaatttcaa tccggtggca tctattatat
    16141 caatttcatt atcgggcaga attttggggt ttatgctatc atcaatagct atgattgtct
    16201 cgttacaaac caggacatcc tttttcccca tgtaatcagg ggcatacact tcagcgttag
    16261 ctataagagt aaacatggac atattttact gttttgggta aattgatacc tgcttatcgg
    16321 attttgaata tccaatagga atagttttgg agataataag cagaacttca tcgatagttt
    16381 cgttatcaac agtcatggtt aacctatact tccctttaag gtcattgcta agcgagattt
    16441 caaccccata ccaacggtta agcttattaa caacactttc aataggctca tcgctgaaaa
    16501 ttagtttacc ctgctcccaa gcggttactt tactaacatc aaccattgaa acctcaaagc
    16561 gattctggtt aaaatagtat gtggccgaat ggtttggggc aagggttaca ctttgctcct
    16621 tttttgtact taggtcaatg tatttcaatt taaccttacc ggaaattaag gttgcagtaa
    16681 ccattccatc ggcagggtaa gccataacgt taaaaactgt acccaaaacc tcaacttcaa
    16741 cattgttggc agccacaata aaggcttgat ttggattatg ggttacctca aaaatggctt
    16801 cgcccgaaag ctcaactcgt cgaacctttt tactgaatac tttggggtac ttaatggttg
    16861 tgtgcttatt aagcaacacc ttcgatccat cgggaagaac aacttgctgc atcgaattgc
    16921 ttccgctaaa aacagttaac atttgtttct cagcaaaatt tctactgttc caaacgaagt
    16981 aaattgctga aagcgatatt gtaaccagca cagcaattga tgcagcaacc ctaaaggatg
    17041 gcttttggta ccatcgaata gttggtacat tattgttatc aatctgatta ctaatttttt
    17101 ggaatagctt ttgcttatcg aaagatggtt gtgaaagttc agaattggta ttattccaaa
    17161 cctgctcaag catactattt gattcgtgcg agttataaat ctcttgcttt tccttttcgg
    17221 aagcaatccc cagcatcatc cgcttcaact gtttcgcttt tagccttaag ctcattgatt
    17281 ttaattttat tggtaaaatt agtcatttat cgtaatacgt gtatacatta catttccctt
    17341 aagcggaatt ggttaataag tattaaataa aaagggctac ctatttggta gcccttttat
    17401 ttgggtcaaa gtaaaggtct accttacaac taacttatta attccggttg taccacggta
    17461 agtggttcta accacataaa taccattacc ccaatctttt gtttcaagat ttactcgggt
    17521 ttggttttcg gttgcgttta tggaagtgct gtaaaccatt cgccctacaa tatcggttac
    17581 tacaacatca accttacctc ccaattgggt ggggacaaga atggttgctt tttcggaagc
    17641 agggttgggg taaacaagtg tattaagcag gttcttggca ttatcgctaa tcccgcttag
    17701 aacatcgttg tagatgtgta tattatcaac cgcaaggttt gaaccaccgg gggaactgtt
    17761 gggaattccg gtatcaggat taacgtaaag gtcggtaatc agtacaaacg caatccaaac
    17821 cggacggttg aaccaaccat cgggagcgct tactgttttt gcattacgca agttaataga
    17881 gcgggtagct aaaccctggc caggacgccc aacgtactgc gaccagctct ccggcttaac
    17941 ctcaagcaat atattgcctt ctgtttcaaa gtatgaaatg gtaggagtaa caccatccac
    18001 tgcaggagca attactacca tgtaatcatc gggataattg cctgaggttg tagttgacat
    18061 agcctgccat gcaatggtgg tattattacc taaatcaacc ttaggtaaaa tcatccagtc
    18121 gtatgccttg ccatccatag gcatatcgct ggtgtacgat gaagccattg caacctgggt
    18181 tccttgtagc tcctgacgat tggttgttgc aaccacccat gcgctatcct gccaacgctc
    18241 ttcgcctggt gcttgtgtgt aaccatcgcc attgattaac acccacccac tgggtattaa
    18301 aatcttattc tcaaaatctt cgaacagcag cgttgttctt ccctctgcag ccttggttac
    18361 tgttacttcc catgcttgcg ttgtgccatc ctgagcggta acagtgtagg ttacagggtt
    18421 ggtgaagttt tgagccacac cacttgcagg tgaaatggta gcgtactgtg aaacagtaat
    18481 ggttggtaca agtgttgtta aatcagttga gaaaggaacc tctgcggtaa ccttatgctc
    18541 agcagcattt ataatacctg taacaggtgg tgtaagacct tcaaaccgga acgaaaggat
    18601 atccttaccg gtagcaggtg cagccttgct aaccgttaca acccagttgg tgtttacact
    18661 tcctaactcc agcttgtatg ttatgggatt tgtaaaatcg tttggtgtaa cgccgctctc
    18721 ctgaaccaca tcgtttacct taacggtggc tccattggta gtaaacgaag ctacaagatt
    18781 ggttaaatca gttgcaaaag gaactgtagc cgttacagtc ttggctgttt tatcaatagc
    18841 cccattaacc acagggttta atccttcaaa cttataggtt aagaattctg gttcggttgg
    18901 ggcttcccac tcctcaaaac taccattctt tatcaggttt gtggtaccac cattctcaaa
    18961 gtaacgggca ttatcggccc agaatgtacc actacccgcc caggcagcat cggtttgcat
    19021 tcgtatggcc acataggcta gggttgcacc tgcaggtgca gttcccgata tggttatggt
    19081 ttgccaatcg gcattatcgg ttgttgcgct acttgtttta cgctcaaggt atgaggtatt
    19141 tggtgctgag aagaaccata accttgccga aatcttacag gctggatcgt tatccaggta
    19201 atcaatgttg aactcatagg tggctccacc ggttacagca aaggtatcgc aaatgaagta
    19261 aggtgttcca tcgtcggtaa aggtatactt aaccgagtag aacccatggg tgctcttggc
    19321 taactcaggt gtaatagttg atgttttgga gggatcgtac aaatcctcac gccaattctt
    19381 aatggtagaa ttttcaacca ctttccaacc ctcaaaacca ccattcttaa ttaagttagt
    19441 agttccacca ttctcaaagt aacgggcatt gtcggcccag aatgtaccac tacccgccca
    19501 ggcagcatcg gtacccatgc gtatggccac ataggctagg gttgcacctg caggtgcagt
    19561 tcccgatatg gttatggttt gccaatcgcc tgagtcagtt gttgctccgc tggtttggcg
    19621 ctcaaggtat gaggtatttg gtgctgagaa gaaccacaac cttgctgaaa ttttacatgc
    19681 cggatcgtta tcaaggtaat ctattttaaa ctcataattt gcccctcctg tcactgcaaa
    19741 ggtgtcgcat ataaagtagg gtgttccgtc gtcggtaaaa acatacttga gtgaataatc
    19801 accctcgctt ttaacagaat tatcgcgagt tatggttgaa gtttttgtgg gatcgtacag
    19861 atcctcacgc cagtaaagcg gctgagcaaa agttgccgct actatggcta caaacagagt
    19921 ggtgagtaaa attttcgact tcataggcat tgaattttaa agtttggtta ctatacatac
    19981 acccaacttt aagctatcac ttaatgggtt gattcttttt tagcctggat gtctacttga
    20041 attttgaatc atcttattat tcctagtcta acaacactta gagaaataat ttttttgcga
    20101 ttaagtgcat gcccctgatt aattgtatta ctataaaact ttgaatatga cagggaaaaa
    20161 catcctttta tacctactac ttggtatagt ttcgctttcg ttcctgaaat gcacgcagca
    20221 aagcgctagc aaaggcaagc tttatattat tggaggtggg aaacgccccg atgccatggt
    20281 acagcaaatc ataaatcttt caaaacttaa ggaaaagggt tacctggttg tgctgcccat
    20341 ggcaagtgaa gaacccgata gcgcggctta ctacgccaca aaacagttta ctgaccgggg
    20401 tattaacagt gccctttcaa tcatttttca aaagggcgat accataaaac agtggtgggt
    20461 tgataccctt aaaaaggcct cgcttattta tatctccgga ggcgatcaaa aaaagtttat
    20521 ggatattgta ggtggcactc ccatacatga agcactggta tacgcttacc ataacggagc
    20581 agttattgcc ggaaccagcg ctggtgcagc tgtaatgagt aaactaatga ttactggccg
    20641 acaacttaaa tttcccgatg atgaaaggta tggccattta gttccaaata acattgagat
    20701 aaccgacggg cttggtttag taaaaaatat tattgttgac caacacttta ttaaacgcga
    20761 acggcttaat aggctcattg ctgtcagcat tgaaaacccc gattatctct gtgctggcat
    20821 tgacgaatct accgcactgc tcatttcagg cgatagtgca actgtttgcg gtataggtca
    20881 ggtggttttg gtagatggat ctaaatctaa agccatttcc agcgattcgc tccttggggc
    20941 caaaggactt aaaattgacg ttcttttacc cggtgagagg tttaaactac cttaattggt
    21001 actacttatg atgtaataaa aaaatggcga agaagttaat cttcgccatt tttaaacact
    21061 attcgtatag tataaacttt tagctctact taaaggctag ccaatcggct taaggcctct
    21121 ttaaattcat ctcgcggtat tgtgatattc atcgccaaat acctatgaca ctgcctatca
    21181 tggtaaaatc ctgaaagcgg cagtaaacca acgcctttct ccgctataaa cttttcaacc
    21241 atttcaacat cacggatatc ggcgtagcct ttataaccaa gcattaccat gtaccccgcc
    21301 gattgcttta cgggaataag tttactatcc ttgagtgtct ccagtaagag cttgtagttc
    21361 tcatccagaa aatcggctag catgtgccct tctatctcgg cgctcagaat attgataaaa
    21421 tcttcggctc ggtttaagtt ggggttcgag gtaatagccg aatggatatt tttataggca
    21481 cccattaatg gttctggtgc aatacagtaa gcaatttcac tatcgttaag tgcaacagga
    21541 atattgagcg aacctatgcg gtaagaaacc tgacaaagtc ttgggtaaaa gttaacgctt
    21601 gttccctcag aactgctaaa gcccagttca gccatagatt cgtttaggat aagcttgatt
    21661 ttggtaccat ttatcaacct ttgcagctcc tcaaaatcct cagctgttag tgtttgtccg
    21721 gttaacagat gaggcgatga aataaccatt aacttggttg tagcattaat ggctcgctga
    21781 acctcgcacc aatcaatgcg gaaatcgggg gctcttaaag gtatgtaaac aggacgggca
    21841 ccacaaagct ctactgctgt ttttaggttt gaagtaaatg gctcaaacac aatcacgcta
    21901 tcgccatcct ttacggttga agcaattgct aaaaaaaccg ccaggttaac accgctaaca
    21961 aaggctatct cggtttcagg gtttatcgcc ctaaagtatt tttttagtat gtactttgag
    22021 agtttctggt atggattcat gctttcatca cgatccatat caagattgta atcattaata
    22081 ttactatcaa taaactcttg aaatcccttc tcgttgggtg ttaaagaagt catatcaatg
    22141 gccctgtact ccctgattag cttttgcaag ctgtcgtcgc ttaaagctgt tatttcgcct
    22201 accttctggg tcatagtgaa ttaaaaacaa tgattgtagt ttaaaattac aaatagtatt
    22261 tcaattatca tatcaatttt gatggaatgc agggtttatt tgatgaatgc agctattcga
    22321 atagctggat tagaattggt agggttctga aacgtattcc agaaccaagg tgaaagcggt
    22381 aaaatgaggc aatggcttca atgagtattt gtcgctgatt ttgatttaaa ctcagcattt
    22441 cagggttgag catgggtgta tcaatcaaat tgctaagcag cttgctggtt tgtttctcaa
    22501 gcacaaagcc gtgagcgggt tctaccccac aaaaaacacc gttttttatg tcaaagtaag
    22561 gctcttctgg actccatcgg tttaaggggt aaaaccctaa ataccgcgaa agctgcacca
    22621 tgaaaatcag atgaaagttt gctacaccgg tattgaggtt atcgagcatt tgaactgagc
    22681 tctcaataaa ggcaaagagg tgctggtttg tttcctgttc tcttaccgtt ctgtaaatta
    22741 actcgctgag aaaaatagta attgtgagct taatgggatt taggtgaagg ctggtgaaag
    22801 cgtatgtagg ggtaacctct tttaccctgt acatctcagc gttagggcga tagtagtaaa
    22861 ccacatcgag aatggatagg ggctggaagt aaacattcaa ccccgccttt ttccccttag
    22921 ggaaagcgtt atttactaac agagtaatac gcccatgctc acgtgcgtaa cagctaacta
    22981 tcaggctatt ctccttaaat ctaacagtcc taagtacaat taccctattg tgctgtaaca
    23041 tgggcttatt ttacaaaaag aagtttagta acagcagcct gctcgccttt tgagtcggag
    23101 caaaatatta ggtaaacgcc tgtagcaacc ctctgcccgt tcctattttt cccatcccat
    23161 gtagctaaac ctccgtttga gcgagtctca taaacaatgt ttccagcaat atcggttatt
    23221 ttaaccacgc tattgtccat aagcccggca atggtaataa ccccactata gttagggcga
    23281 accggattgg gaaaggcgta aaccttatca aacgtattag ctggctcact ggcattgcta
    23341 cggtaggcaa tcaagccctt atcggtggca ataaacacct caccggtaac cggatgcgct
    23401 ttaatgctta gtattgtatt tgatggcagg gggctattct tggtgttaaa atgatgtatc
    23461 agttttgtcc catcggctga aaaaaggtaa acccccgatt tagccgttcc aaaccattta
    23521 cggttgcccc catcaacatc aatacacgta acctcctcgg tttccagaag gtaaactgcc
    23581 agtccttcca ccacatcggg tatcttgatt ttttggaaac tggtttcacc ctgtaatacc
    23641 ctatgtgggt tgtaacttac caaaactccc tggttggtcc ctaaccataa ataaccatcc
    23701 ctgtcaaatg caagtgcagt aacctcgttg gaaaataaat tgccattggc gtcgcgcggt
    23761 cggaatttaa ggtagatatc gtcacttgcg gcttctacgt cgttaccggg gctaaggaca
    23821 aacaatccat tatcgcgggc taaagccaac cagattatcc ctgatggcga aacggtaagt
    23881 gaaataatcc ttgaggttcc aattgctgag taatacggaa acgacttcca taccccctgt
    23941 gccgtaagca ccgatatggg atttggaacc tcagaatttg ctacccaaag gtttcccttg
    24001 gggtcaatat ccataccggc tattctacag tatggttgtc ctggaaatac actttgcagg
    24061 gtgctattat cgggattata atgctccgcc agggttccac tattaagttt atacagacct
    24121 ccaccccagc ttgcaacaaa aaaaacattc gggttacggg gatcagcctt caccctaacc
    24181 gcatcgtgat taaaccaatc agcatagtac gaccatcggc catcggaaaa agtatgtgca
    24241 ataaacgggt tccaaaaatt cccgaaagcg gcatcgtatg cacccgatgc cgccacaacc
    24301 atgtttgcac ttgcatcaat gtgaaaaaat ttgttgctaa acgggcccgt tggagtatga
    24361 acactatgat ctgtagggga tataatcact aaaccattga attcatcggc aacagccaaa
    24421 gcattgtttt caagcggtat acaatcggca acttttggag tgccaccaaa aaggtaactg
    24481 cttacaagtt ctaacttctc gccctgcaag ctatatttct gaatagcatt actgctaaag
    24541 gcagcaattt tctgaaagcc tatggataca ccaagggttt cgtcaaaagg cctatcaacc
    24601 tcaacccatc cagaaccaga attcatccat agaatatcgt taccaccaac tttttccaaa
    24661 gccaacaagt gggtttcatc agctgtaacc cctctgcact ctgctgcagg gtcagtaaag
    24721 aattgctccc tgctccatcg ttcataactt attagcaaag ggtcatttgt acttgcgctg
    24781 taaacaccct ggcttgtagc tgcccaaaac tttccattcc aggcaaccaa cctgttgacc
    24841 tttaaggtac cagcgttttc aacaataaag taggtatcct ttacctcaag cctatcggga
    24901 tcaattacca ctaaaccaaa atcggttgaa acgtaaacca atgagctgaa gtagtaaaaa
    24961 tgatttatgc acttgctgcc caacatgggc ttatccttaa tgaatggcaa gctgacgacc
    25021 cgcttggtgt tttcatacac taaatcaatg ttgccagatt tatacccaac taacgttatc
    25081 tgcttttgtg gtattgctga taatgcagta atatcaacct cgcttaatcc ggaaacagtt
    25141 gtgtacttgt tgagctcgcc gttttcggta tcgtaggtaa agtagccatt tgccactgca
    25201 cagtaaagct ttgaaccgct aatgctaagc tcacgggcat cgttaaaggc atagtaatcg
    25261 cgccactgcc ctatggcagg ttgggtgtga accttgcaaa ttcctacaaa tatgaaggat
    25321 aaaaggatat atatcctctt taccatagct aaagatttat gttacccggt gtaactaaag
    25381 aataaacgat taatgattga ttaatattgc taaaacctgc tttttaggaa tttgcataac
    25441 tcctcaacgg cttttcccct atggcttatt tgatttttta gcgataaagg catctcggca
    25501 aacgtttcgg taaagccatc gggtacaaaa attgggtcgt agccaaatcc atcggctccc
    25561 cgttcctggt caatgatttt cccccaaaca actccctcaa aaagatactc ttcgccgtta
    25621 agtattaatg cgataacagt tctaaatcga gccttacggt tttcaactcc tttaagatta
    25681 ctaagcaatt tacgtatgtt atccttcggt ttttttgcat cgccagcata gcgtgcactg
    25741 tacacacctg gctcccagtt taacgcctca acctcaagac cggtatcatc ggcaaaacag
    25801 ttttgaccgg ttttgttgta aacatagcgt gccttttgca gtgcgttatc ctgaagggta
    25861 ccatattcct cgggtatttc gtcggtaata ccaacagcgt ttaacgtaac cagggtaaat
    25921 ctatccccaa gggcatgctg tacctcttta agcttgtgtg gattgttggt ggcaaacaca
    25981 agcgaaaaac tgctaccggc cataagtgaa ttatcgggga ttaatactca ttacagggat
    26041 atgccttgca ttggccagaa tatattgctc ctcgggttca acaatatatt taccatcctt
    26101 actggttgga gtggtcataa taattattag gtcagctcta atattaacgg ccatgtccag
    26161 cacttcctcc tcatacttct taatgccggg aacggtatac tccacgtact tggcgcccct
    26221 ctcatctaaa aatttcttgg ccgaagccag gtttatatca atctgtgcca tcaacttggg
    26281 atccgatgta ttgggcttaa tgatatggaa acgggatagg tagtaatcac agaagtacga
    26341 tatccactca tgtttctctc tgttttcaat ggtaaagtcc acagggaata caatatcatc
    26401 gtaacgcttg ttaaccggtg gttcctgtat ggttataaag ggaaccttgc agctggtaat
    26461 taactttagc acccacgaaa ccttatgctc atccatctcc tttacggttg atgagcccat
    26521 aattaccata cttgcattta tttcatcggc tgttgaagct atgtcggtca tgtagttacc
    26581 tactttgact atcgattttg gagtaacagc gtacttgctc tttaccgatt cgataagtga
    26641 agccatttta ctttcggctt cagcaagttt ttctggctcc gaaacacagt gtaagatgtg
    26701 aattggctcc ttaacaactt cagaaaccct tatggcatgc tgcagggcaa accatgcaac
    26761 ttccgtaaaa tcagttggaa ccaaaattga ttttttagca gtcataattg tcgttatttg
    26821 gcttttcccc taaaacatta cgttcataaa tcaccttcaa atttaatcaa attattattt
    26881 ttattgagtt caaaagaaat ttgtttaaac aaattgttaa aatatttgat aaagattcgc
    26941 aatcgctcta tcttagtatg tgaaattttt tgcgttttac caaatggctt tcctttcaac
    27001 cctaatagcg ttttctttat tggcagtagc aactcttgct gctatgatgc tgtatgtacg
    27061 ttactactac accaaaaaga gttttgccct tcagcagaag ctctttgtta gaactgagga
    27121 attactcagg gaaagacaga aaaccgaaaa cttgctctca cgggttttgc cccgcgaaaa
    27181 ggctaaagaa ctaattgacc agggaaaggt tgatgcacag aaatttcaga tggttacagt
    27241 tctctttgcc gatattcagg gctttaccaa aataaccgat gaaattaatg ccgattcact
    27301 cattgaccag ctcgacaggc ttttctttga attcgatagc atagttgaaa agtatggtat
    27361 tgaaaaaatt aagaccatag gcgatgccta tatgtgtgca ggtggcattc cccatccaaa
    27421 cagaaccaat ccttttgagg tggtagcagc agcagtggaa atgatgggtt gcatgcaaaa
    27481 gttaaaccag cagctcaata acggcaaaga aatctgggag ctaagaattg gtatcgatac
    27541 cgggcctgtt attgccggga ttgttggtcg taataagttg acctatgaca tctggggaac
    27601 cactgtaaat attgctagcc ggatggaatc ggcaagtgag cccgggaaaa tcaatatttc
    27661 agaaaacacc tatctggttg ttcgcgatct attcgaatgc aagttccggg gaaaaattcc
    27721 aatcaagaac aagggcgatg ttaacatgta ctttgttgaa ggattcctac ctaaattttc
    27781 cgagtccagc aggtatatcc caaacaatga ttttagggtt gagcttcagc ttgtaagatt
    27841 agccgatctg gaagaatttg ttcttgagaa gctggaaaaa ggtctcccaa aaaatcttta
    27901 ctaccacaat ttaaaacaca ctgtcgacgt ttatacacaa gttgaactta ttggtcgagc
    27961 cgaaaatata cctaatgaag atttgctctt agttagaacg gctgccttgt ttcacgatat
    28021 gggccactta attgactatg caacgcacga ggaaatgagc gtgaaaatgg cgcatgaaat
    28081 attgccccgc tatggctata ctcccgaaca aattgctcgg gttgctgaaa tcattatagc
    28141 taccaagtta cctccaaaac ccaaaaacat cctggaggaa ataatttgcg atgccgatct
    28201 ggattacctt ggccgaaccg attttatacc cgtatcgaat gctctttaca aggaactcca
    28261 tgagcatgga atggtgggtt ccttacacga ttggaatttg attcagattg atttcatcca
    28321 gaaacaccag tattttactc aaacagcccg tagattaaga aatgtaaaca aggatgttca
    28381 gctcaaaaat cttaagcgat ggatgaacga attagaaaat gatacatctt tgtagtgatt
    28441 tttacatttt tttgtattac ttttactatt ttgttaaata tttgctattt tgcatagtta
    28501 aagttgaaat actgaccaaa agttccggtt atggataata ttaaaaaaat cattctggtt
    28561 ccctgggatt ttaccgaagg ttccgaaatt gcctttcaac atgcccttca gctcgcaaaa
    28621 gccggaggta atgagatcat gttgctacac atcatccccc gtgcctggat tattgactcc
    28681 atataccgta aggtaaattt caagaagata gaaacctata atgccaaaat tgaaaaggct
    28741 gcttcggagc ttgaacataa gtatggtttt aaaccacaaa cccttgtcct ggaaggatac
    28801 cctaaacgta ttatcaaaga gttaattctt acagcccatg ttaaccttat tgtagcctac
    28861 tatgcctata aatttggcag taaggagcac cgcatatcac gataccttaa aaatctattt
    28921 ttcaaggata caacccttcc cttcattgta gtcagcaaag ccccaatcca ctcacactat
    28981 attgaaattg taattcccgt agattacgac cggaagttca aggaagcagt tcactggatt
    29041 atttaccttg ctaagtacta taagtgtaac gttaacctca ttaagccttt cctaactgat
    29101 gatggcaaaa agaaacagtt ggccaacaac atctacttca ccaaaaaaat gctcgatggg
    29161 gttaatattg tttacggtat taaaaccgcc aagaaaaagg gaacttttga agaggaagtt
    29221 ttccgctttg cccgcacaat tgaagccgac ctggttctta tcatgtccga caagtatgac
    29281 gatttcctcg atagcaagca ggaagctaaa catgatatcc ctgtaatgtg tgttaatcct
    29341 cgtattagaa agtatcaaag tttttattaa taggttttta tgaagctacc aagaacgatg
    29401 cttttaactt caatcatgaa gttgtcgtta aaagaaaaaa ttatttttct attcaccgga
    29461 acatcgtttc ttattctttt tttccttctt ttcctaaata gcgctaggtt taaccgccta
    29521 tccattgaaa cagccaaatc gcgcatttcc cttttagccg ataaggaggt aaaaaaattt
    29581 gaggcaacac tggaaaggga tatgtctgtt gcacgcacca ttgcctcgtc aatggcagta
    29641 accataaaag aggggaagac atcaaatata caggaataca acaatctcta caccaacata
    29701 cttgagcaat accccgatgt actttccgta tgggatagct gggaacttaa atttgttgaa
    29761 cccggatata cctacgaaca cggccgcatt aaaaattacg cgtggcgcga aggcggtaag
    29821 atactggcaa atactataag aactgaaaaa actgaatcgt cagcggagta tatcactcta
    29881 aaaacaaaca aatcggaaag tttggaggaa ccctactttg acaactacga gggacaaacc
    29941 gaaaatattc tgatgaccag cgttattgct ccctgcatta tcgatggtaa ttttgccggg
    30001 gtggccgggc tcgacattcc cctttcaaac taccatcagg ttgttaagag cattgtaccc
    30061 ttcaaggata gctacgcttt tatcatttct cctcagctca agtttattgc ccatcccgat
    30121 aaggatttga ttggtgacga tgccctgttt tcctacgagt ccatatttac ttcacatggc
    30181 gttcaggaca aggttataaa tggtgaaccc attgaatttg aagcaacaga tatcaacgga
    30241 aagttgtcat tctttacttt ccgccccatt tcagttggta agtgcccaca acactggtct
    30301 tttgtactga ttacaccgcg cgaaaccatt ttagcagagg ctcgccatgt tttttggaca
    30361 acccttacta ttggtttaat aggcattttg ttgctttctg gctttattta ccttatgacc
    30421 cttaaattcc ttatcaatcc aataaataaa gttacccaaa atttagagcg cctctctaaa
    30481 ggtcatgttg acaacagcat gattgttgat gttgacaaca acgacgaaat aggcaccatg
    30541 attacccacc tgaacaaaac cgttgagggc ctttacaaaa aaaccgagtt tgctaaacag
    30601 gttggcatgg gtaattacga cacctcactt gaactcctca gcgatgagga tgttttgggt
    30661 aagtcgctga ttgaaatgaa tgagaatctc cgcaaggcaa gggaagaggc gctaatcagg
    30721 cagcgtgacg aggaaaaacg tcgttgggtt aatgaaggtt tagccaggtt tggcgatatt
    30781 ctccgacaaa ataataatga cattgaggcc ttaagcgcta gcattgtaaa aaatttggtt
    30841 ggcattctta acgccaatca gggcggactt ttcatttata acgatgagaa caccgatgag
    30901 ccctactttg agctaactgc cgcttatgcc tataaccgtc aaaaatttat taaaaaacac
    30961 atcctacttg gggaggggct agttggcacc tgtgctcttg agaaacaaac catctactta
    31021 aaggaaatcc ccgagaacta catagcaata tcctctgggc ttggtgaggc aaaaccacgc
    31081 aacctgctta ttgtacccct aaaggttgag gataaaatat ttggcgttct tgagctggca
    31141 tcgttcaatg agtttgagga ctaccagatt gagtttgttg aaaaggttgc ccagagtatt
    31201 gggcaaacac ttcagtccgt acgaactaac attcgtacag cggaactcct ggctaaaaca
    31261 cagcaacagg ccgaggaaat gaaggctcag gaggaagagg ttaggcaaaa tcttgaagag
    31321 ctggagacca ttaaggagga gctagagaag cgtactgccg aaatggagga aaaccaaaaa
    31381 cagctggaat gggaaaaatc gctgttggac tccctgctta actaccttcc cgataaaatc
    31441 tactttaagg atctaaaaag tcgattcatt aaggtaagca aatcaaccct cgaattcttt
    31501 ggaatgtcaa agcaagaaga gcttatcggt aaatccgact tcgacttctt tactgaggaa
    31561 cacgcccgcc ctgcttatga agatgagcaa cgaattatta gaaccgatac gcccattatc
    31621 ggtattgttg aaaaggaggt aatgaacgac ggtagggtaa cctgggctga gacctcaaag
    31681 ctccctctaa aaaattccca tggcgaaacc attgggactt ttggcataac ccgcgacatt
    31741 accgcttcca aaatgatgga ggaggaaatt tcaaagaaat ccgaaaagga agaagccttt
    31801 cagcgcgacc ttgagaaaaa ggtgattgaa cttgaaaacc tctataaagc cataaacgat
    31861 tccgcttacg ttattgagta tagccctgaa gggaacgtta ccttcattaa cgaggcttac
    31921 cttcagctgc ttaaccttaa cgccgatgat gttattggga agcaccactc ctaccaaatg
    31981 gaatttactg atgaacagcg taaaaactac aaacagttct gggacgatct taataatggc
    32041 attgttagaa aggaaacctc aaagttcagc attaacgata aaacctacct gttctacgaa
    32101 acctacaccc caataaggga tgctgaaggg aatgtggtca agatcatgaa aattgctttc
    32161 aacgttagcc atttgctttc cgaaaatcaa atataaagcc gggtttaccc ggttttttta
    32221 tcctgtaatc tatgttaagc tggaaaaaac ttttaagccc aataagaacg ggcgaatcgc
    32281 catcaaaatc gactattgac tatacccgaa ccaactttca gcgcgactat gataggctga
    32341 tcttttcctc cccgttccgg cgtttgcaaa ataaaaccca ggtttttcca ctcccgggta
    32401 gcgtttttgt gcataacagg ctaacccata gccttgaggt ctccagtgtt ggccgatcgc
    32461 tagggcggag catactaaac cttttatcac ccgaaaggtt tgagtgccca gcacagctaa
    32521 ttaccgatat tcctactatt gtttcggcgg cttgccttgc ccacgatttg ggaaatcctc
    32581 cctttggcca tagcggcgag gatgccttta ggcatttttt ctccaaccat cacattcctg
    32641 aacttgatag ctgggaagca agcgacctgc tccggttcga aggaaacgca aatgctctcc
    32701 gcctgcttac ccaccagttt aggggccgac gaaccggcgg tttccgtctt aactatgcaa
    32761 cccttgcctc aattgttaag tatccttacc cttcaacatt gaaagaaaaa aagtttggtt
    32821 attttaaatc tgaagccgat gtttttaacg agatagccag ccaaacgggt cttattcaga
    32881 ttagcaatgg aagatattgc cgtcatcctc tggtttacct ggttgaagcc gctgacgata
    32941 tttgttacca ggtgatggat attgaggatg cccacaagct aggaattctt tcaaccagta
    33001 ctgtaactaa cctttacctc tccttttttg acgatgaaac cacaagtaag aagaaagata
    33061 ttcatgaaac cctttcctcg gttaacgata taaacgaacg cattgcttac ctaagagcta
    33121 ttaccatcag taaacttatt gagcaatgtt cttccaactt ttcaaaaagc tatgaccaaa
    33181 ttatgtccgg ccagcccgta aagccgctta ttgaccaaat ggaaggaaca tctgcaaagg
    33241 caatggacga aattcggaaa gtttctgtta accaggttta caaccattcc aaggtggttg
    33301 aaattgaaat tgctggcttt accattattg gggaactact cgatattttc acaagtgcag
    33361 ttcttgagcc taccaagcca atttcaaaaa aagccctcag gctgataccc gaacagtacc
    33421 ttgctgaaag ctccgataat tactccaagg tgatggctgt tgttgattat atttcaggca
    33481 tgaccgatat ttatgccctt gaaacctatc agctttttaa agggattaaa ctaccctcgc
    33541 tttaatctta cttctcgcgg cttaccatat aatcagcaag gagcaatagt gatttcttat
    33601 actccgaatc gggaaatatt tcaagtgttt gtttagcatt atccctaaac tcaaacattt
    33661 ttttggaggc ataatcaatt ccgcccttgt ccttagcaaa atcaactatc tcagcaatgt
    33721 ttgctttctt cccactatta attagtctga taattcgtct cctttcatgg gtatcggatt
    33781 gggaaagtgc ataaattaaa ggaagtgtaa gcttgtgttc cttaatatcg ttaccggtag
    33841 gcttgcctat taaccctgat ggctggtagt caaaaagatc gtcgcggatt tggaaagcca
    33901 tgcccagata tttcccaaat tggtaaattc tgtcaaccat atctttggtc gcattggctg
    33961 atattgcgcc attggcgcag cacgaggcta tcagcgatgc cgttttcttt ccaatgatat
    34021 cgtagtaggt ttcctcatca atattcatct ttctggatcg ttcaatctgc aaaagttcgc
    34081 cctcgctcat ttcctttaca gcactactaa tcaccctgag catctcaacc tgaccatttt
    34141 caatagcaac cagcaaaccc cgcgacagga taaaatcccc cacaagtact gctacctttg
    34201 aacgccatag ggcatttatt gaaaaaaatc cccgccgttc ataagcttca tctaccacat
    34261 catcgtgaac cagtgttgca gtgtgaagca attcaatcat ccctgcagcc acgtaggttg
    34321 actggtttat ttggccacaa gccccagcgc tcagaaatac aagcattggc cgtaactgct
    34381 tgcctttccg cctcacaata tagttagtta tcagatcgag caattttata tcggagccca
    34441 tgttttttct gaaaaatggc tcaaaatcat tcaaatgatt acttattggc tcctttatct
    34501 ggtttagcat aaatctttat ctattaaagg gcaaattaac caaatttcgc ccatactaaa
    34561 cactgtttac cctaacaatg tttaaaaaat tgataaatac caattggtgg taaaacaaaa
    34621 tattaatata tttgcatgtg tttaatatga ttgaaattca gaacaataat ggtacaggtt
    34681 gaagaattga agattgagca gcttgagcag gctgcaagca tgttgaaagc tattgcccat
    34741 ccactccgaa ttggaattat tagcttgttg gaaaaatcgg ggcagttgac cgttacccaa
    34801 atatatgaaa ctcttggtat agagcaatct actgcatcgc accatttggg tattttaaag
    34861 gataggggtg tactttcatg caagcgtgag ggcaaaaaca ctgtatactt ccttcggaat
    34921 gagaatctgg gtaagatact tgactgtgta ggaaggtgta ctgtttaata attaaattat
    34981 gaccactttt gatatcataa ttgtaatact actattggtt ggtgcagtta ggggttttat
    35041 tagtggccta attatgcaac tcacatcgct ggcagcgctt ttacttggca tttgggtagc
    35101 aatccggttc tccgatttta ctgctatgct tttaatggaa aaggctggtt taacaggtca
    35161 gtacattcct ttagtctcat ttgccattac ctttgtggtt gttgttattg ctgttcactt
    35221 taccggaaaa ctaatcgata aattttttaa cctaacccca ctgggtatag ttaataagat
    35281 tcttggcttg gttttcggtg tactgaaata tgcattaata ataagcgtaa ttattgtttt
    35341 tgttgaaaaa gccaatcagc ggttcaactt ttactctgaa caaaccaaaa ataaatcaat
    35401 gctatttaag cccctttcaa agcttgcccc tagcattttc ccttaccttc actttgaatc
    35461 gatttctgag cgcttaaaag cgaaataatg caccatttag tcagattcat ttcggctatt
    35521 tactttaaat gaactatatt tgaaggccaa atcatttggc cttttttgtt taatcactta
    35581 aaatggtttg ccgtgaattt tgtagaggag ttaaaatggc ggggcatggt acacgacatg
    35641 atgcccggaa ccgaagagca gcttactaag gaaatgactg ccggatatat tggctttgac
    35701 cccacagcgg agtcgctcca tgttggtagc tttgcacaaa tattgcttct aaagcggttt
    35761 cagctagcag ggcacaaacc aattgcactg gttggtggtg caaccggtat gattggcgac
    35821 cctagtggaa aatcgcagga acgtaacctg ctcgatgagg aaaccctaca gcgcaacctg
    35881 gagggtatta aacgtcaact ttcaaagttc ttggattttg agagcggtaa acccaactcg
    35941 gctgtcctgg ttaacaacta tgactggatg aagggttacg actttcttag ctttatccgc
    36001 gatgtgggga agcacatcac tgtaaattac atgatgtcca aggactctgt taaaaaacga
    36061 cttggcgagg agtccaagga aggtatgtcg tttactgagt tctcatacca gcttgttcag
    36121 gggtacgatt tcctttggct ataccaaaac atgaactgta agctgcaaat gggtggttcg
    36181 gatcaatggg ggaatatcac taccggaaca gaactcattc gccgtaagct tagcggcgaa
    36241 gcctttgctt taaccattcc gctcatcacc aaagccgatg gcactaaatt tgggaaaacc
    36301 gagagtggta atgtgtggct cgatgcacgc ctcacttcac catacaagtt ctaccagttt
    36361 tggcttaaca cctccgatga ggatgccgaa aggtatatca agatttttac cctgctcgac
    36421 aaaaccacca tcgatagcct tattgctgag cataaaaagg agccacacct acgtaagttg
    36481 caaaaaacac ttgcccgcga ggtaactgtt atggtacatt ccgaggacga ctacaacaaa
    36541 gcagtggaag catcggaaat gctatttggc aatgccacca ccgatatgct taaccggatg
    36601 gatgaggata ctttcctttc cgtttttgag ggtgttccca cctttgaggt taaaaaatcg
    36661 gagcttgcca agggtattcc cttttcagtt cttgctgccg aaatcacaaa agtattccca
    36721 tcaaaagggg aacttcgtcg cctggttcag ggtggagggt taagtatcaa caagcaaaag
    36781 gttgacaatc ccgacatcac aattggtacc gaaacattac tcaacggcaa atacatactt
    36841 gttcaaaaag gaaaaaagaa ttacagcatt atactagctg ttgattaatt ccgttatata
    36901 tttcggaaaa tctttgaagg cttgcagcat tgttttaagg caagcctttg gttttcttta
    36961 ggcaaattat gaaatttgga tatcaagcat tctaattcat cagctatgga caataacgat
    37021 attagcatgg acaaatcaat gctttccatt gctcaaacca tatggaatgg ccgaaaaacg
    37081 ataattactg taggaattat tgcgataata atttccactt ttgcatcgct tttagtaaaa
    37141 aatcaatata aagccactgc aaccatattc cctccggtgg caaatcaggt gtccaaggaa
    37201 ctgattaccg aaaataaaca ggaaggttta acaaccttcg gcgaaaccga ggagatggaa
    37261 cagttccttc aggttttaag ctcacgtacc ttaaaggata aggtaatttc aaggttaaac
    37321 ctccagcaac actggggaat cgactccaca tcaaagagct ataggtttag gagttaccaa
    37381 aaatttgatg aacaagtcaa aatgaaacct actcgctatc aaagtatcaa tattgaggtt
    37441 atggatcgtg accctgaatg ggcagccaaa attgcaaata ctgtggttga gtattccgat
    37501 agcctaatgc gtgccattaa agcacaagtg gctgttaatg ccttaatggc attggaaaag
    37561 caatttgctt tagttgagca tgatttaaat aacttacagg attcgctagc acttgccata
    37621 tctaatgggg ttgttgaccc caaacgacag gcaaccatat tctacaagga atacataaat
    37681 gcattactaa aagctaataa tgctcacctc ataaaattga aaagtgagat gtatcgattt
    37741 agtgatcacg gtgcaaagag tatacgttac gctgcagaaa ttaaagattt ggcagaacgt
    37801 ttaaacgagt tacgaaaaag catagttgtt gctagaattg aggcaaatca ggagataccc
    37861 acccagttca ttatcgacaa agccgatata cccgacaaga aagcctatcc aaagcgcagt
    37921 ataattgttg taacctcaac cttagctgca ataatttttg caattttctt gcttttcctt
    37981 caggagtatc ttaagcgctt aaaaatataa ttgttgatac cttttgcagg atcagtttac
    38041 cccctaatgg atatcattaa atcgatatta agccatatta aaaataagtg gataattgtt
    38101 tccagcataa tcttcatttt aataaatgct attctgatag gaactgagcg cgattttttt
    38161 gtaattctac ccatcatatt tattttttta ctggcttacc tatctttccc cttaaaattc
    38221 tacttggctt acttatttat aagcccgctc tccattcctc tatcggaatt tttccaggaa
    38281 atatccattg atttatggtt accttcggag ttaatgcttg tggcattagc tcctattgcc
    38341 ttaattatgc ttttatcaaa taatcatcaa aaccgttggt ttacttggca tcctgtactg
    38401 ctaactattt actttttttt aggatggatt gctttaactt ccattaccag ttcaatgccc
    38461 ttggtttccc taaagtattt aattctcaaa acactttact tcatcgttct tctttattta
    38521 cccttcatac tacttgttaa ggggaatatt acattcaaac gatttattgc tttctactcc
    38581 ataggtttgt ttgtagtaat tctgttaact ctatcaaagc aatgggaata tggtttgttc
    38641 aataagtttg tagcccacgg ttcatgtaat ccattcttca ctgaccatac atcatatgca
    38701 gcagtattag ctttgctaat tccagttagc attgctctac tcatatcatc caacacggca
    38761 acgcggagaa tacttttcct tgcattaaca attgttctta ttgtagcctt tgtgctatcg
    38821 tacacaagag cggcctggct tagcttaatg gtgacaggtg tagtgtgggt tgcatggttg
    38881 cttaaaattc gattccgata tttagctctg ctgacaataa ttttttttgg gtcgatattc
    38941 tatttccagg acaacatcct aatttggcta aaccagcaca aaaccgcatc gagcggtgat
    39001 attagaaagc atattagctc tatcgccaat attagaaccg atgaatcgaa tgttgaacgc
    39061 cttaaccgtt ggacttcggc aattagaatg tttgaggaaa aaccgctgct tggttggggc
    39121 cccggtacat acatgtttca gtatgccccc taccaggctt cgtatcttaa aacccgggaa
    39181 agctcaaacc ttgggctaaa agggaatgct catagcgaat acctaggact gttatcggag
    39241 gcgggtcttc cagcagcttt ggcatttatc ctcattattg gcatactgtt ttacaaaggt
    39301 tttaacctag ccctaaagtt aaaacccaaa agcgctgaca gggtaatcct aataggcgta
    39361 ttacatgggt taataaccta tgatgtgcat ggttttatga ataattttct ggatatggac
    39421 aaggttgcag ttcttttctg gggttatgct gctttaatcc tggccatgga gcacctttac
    39481 tcaaaatcga atcctagaat cgaaatatca tcaaaaacat ccgcatccga ttccctctcc
    39541 tgagcaatgg tgtttattat ttgctgcaca ctatggtttc gcgaaaagtt cttttctaaa
    39601 atccttgtgt tgtaaaccac cttatcgcca tcaatggttt caactagtcc atcggtgtaa
    39661 cagagcaaag tgcttggctc atctatattt agaacgccct gattgattat tggtatatca
    39721 tcaatcattc ccaacccaac acatcccgat gaaagctgaa ttattttact ggaatttcgg
    39781 acaaacatga agggtggatt gtgaccagca ttaacatatt ccagctttcg ggtttggttg
    39841 ttatagcggc caataaacat ggtaataaac ttttcgcctt tagccgtagc cattactctc
    39901 tcattgagtt tttcaaccaa cgctgaaagg ctaatatcct tgctgaataa cgccctgagg
    39961 tttgcctgga agtttgacat tagcaatgca gccgaaatac ctttacccga gacatcggct
    40021 atacagaaac ccatatcgtt agggctaagg gcaataacat cgtagtaatc gcctcctacc
    40081 tcaaagtgtg ggctatagta agaaccaatg ctaacacctt ggaatttggg catatcggca
    40141 ggcgaaggaa taagcattga ctgcatgcgc gatgcaagtt ccagctcctt acgcattgcc
    40201 tcctgctcca aacttttatg gaataaacgc aggttctcaa tggcaacaaa aatgatgatt
    40261 gatagggttt gaacaaagtt caaatggcgg atggttgnnn nnnnnnnnnn nnnnnnnnnn
    40321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    40381 nnnnnnnnnn nnnnnnngca acaaaaatga tgattgatag ggtttgaaca aagttcaaat
    40441 ggcggatggt tgcgcttact cctttctcat attcattggt atcgcctatc agcacatacc
    40501 ccaccggttg attattctga attaccggta ttattacatc aagctcgagt agtatgggat
    40561 tttccgatac tcctacaaag gtgatctcgt taaagtgaag taaatcgcgc tctacattta
    40621 tattctcaac aaccctttcg gggcaattgc tattaagtag cagttgccac ttgccctcct
    40681 ctatcttgta aagcaatatc ctgtcgattc ctaaatcatc gttgaggata cgcttaaacc
    40741 tgtcgataag ctcctcggtt ttcaactcag cgcttatggc ctgagccata cctaggagcg
    40801 cgtttagctt aaacgtactt agttttaacc tattttgcga ggcggttatc gacataaaat
    40861 agttttaacc taaaaaaata acttattcca aaagaggaat aagttactac aaaattacta
    40921 ataaaacaat acccgactaa attattttga aattacttta ccctttccga gtattccctt
    40981 gtgcgggtat caattttaat tttatcgcct atgtttataa aaagtggaac gttaattacg
    41041 gctccggttt caacagtagc tggtttaagt gctgttgacg atgcagtatc gccttttagt
    41101 ccgggctcgg tataggttac ttccatttct acaaatggtg gcagttcaca ggtaagaata
    41161 ttctcattat cggcttcaac catcatttca acatactgtc cctctttgag taaatcaggg
    41221 ttctcaatct tgtactcatc aagtgatact tgctcaaagg tttcctggtg catgaaatga
    41281 taacccaact cgtccttata taggtattga taaggcctgc gctcaaccct aacaacatct
    41341 atttttaccc ctgcattaaa ggtgttctca attacttttc cgttctcaag acttttaagc
    41401 tttgtgcgaa caaatgctcc ccctttgccg ggttttacat gttgaaattg aacgatggtg
    41461 taaattttcc cgttaaataa aatgaacagt ccgttcttaa aatccgccgt agttgccata
    41521 tatttctatt ttacaatggg ttgcaaatat agtaaaattg aaaataataa aaaaattaat
    41581 agctacctgt taacataaac tttaggtaga ttgttaaaaa tgcaaccttg aattaatttt
    41641 ggtattactt gttctctaag gcagtaaatt cgtttgccag gttcttgtat tctattaaat
    41701 cgcagtaaac tgaaccaaca aatatttcga actttattct gattgggata aagtttgagt
    41761 cgttggtaag ccatatggtc aagtcgtcct tggttttaaa aaccctaccg gttataacaa
    41821 caggcatgaa cttcatgcac tttaccatgc caaattttgt ttttatggtt tcatatcctt
    41881 taaaacggac ggtaagaggg tacttttcat cggtgaaata ggtttcaacc gtaatggttt
    41941 gatcaggctt tagcggtggc atggttagca tcttatttct caggtagtaa accgatgaaa
    42001 gaatatcctg gcacccttta acaaccacca cctttccggt tttggaacta ataacaatcg
    42061 aggagtcggg tcggctccag tgatcccagg tttgggtgct gtaatgccgg tannnnnnnn
    42121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    42181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngtaaactt ccatgcgtcg agcatcaatc
    42241 attgggcaaa gtattgcatc agcaggtata ttgggatggg atagagataa acctaccgcc
    42301 attgcctgta agctacaaac cccaataagc gggattcccg atgtatagca aattcccttt
    42361 gccattgaaa caccaatcct caaaccggta tacgatccgg ggcctttgct tacagcaacc
    42421 acactaattt ggcttggcgt taaactagca tcggttaaca catcgttgat aaggcgtggc
    42481 aacatggttg aatgagcctt ggggtcagaa agctctctga ccccaagtat tccattttca
    42541 tcgcctattg caactgaaca aacagatgtt ccagtttcaa cgcaaagtat ttttgccatt
    42601 ctaaaaaact gactactcgg ttttaaaaag ttcgtctttg tttttaactt ttctgattaa
    42661 cgacttgtcc ttaagcttac gcgatattgc gctatagggt gcggtaatta cctcctcatc
    42721 gccctttagc ccttcaataa tctcaatata ctggttatcc tgtatgcctg tctttacttc
    42781 aactattcga gcggtatcgc ctttggttat aaaaacaatt tccttaggct tctcaacctt
    42841 ggttggtttg gattcaacgg gttgggtgtt attttcaacc ttttcccatt ctcctgtttt
    42901 ttttacagta tcaattcggg ttgttaccgc aagtatcgga atggttagta cgttttcctt
    42961 ggtgtttgta agaatttcaa cagtagtgga cattccgggt cggaagggac tggtttgtga
    43021 attggtgaga aggtccttat atgatttttc aagaataaat attcgcacct cgaagttggt
    43081 aacctgatcg gttgtaattc cctgaacatt agcggtattt gctatctggg ttactattcc
    43141 tttgaattta cgacccaggt atgcatctac ctcaattaat gcagtatcgg caatcttaac
    43201 ctttactata tcgttctcgt taacgttaac ccttgcctcc attcggttta ggttagcaat
    43261 ccgcatgatt tccgtaccag ccatttgtat agtacccacc acacgttcgc caagctcaac
    43321 gttcagcttt gatactgttc ccgatattgg tgcgtatatg gttgttttgc gaagattttc
    43381 ctcggcttcg cgcaacgatg cctgagcgct ttcaacgcta taattagcag cttttagctc
    43441 agcttgtgca acctcgtagt tggttcgtgc agtttcaaac tccgactccg atattgcctt
    43501 ttgttcataa agctgtttgc ttctattgaa cgacaactcc gactgggcaa gttgggcctt
    43561 agcctgaatt aaccttgcgt ttgcagagtt aacagcggca attgctcttt cttttaatga
    43621 aatatatgta tcgggtttga tgcggcaaag cagggttccc ttttcaaccc agttcccttc
    43681 ttttactggt aattcaataa tttcgccaga aacctctgag ctgattttta cttcaacctc
    43741 aggttgaatc ttaccattgg ctgtaatagc ttcggtaata gttctgtact caggtttctc
    43801 aacataaacg ggtgtggtta agggtttacc tatccaaccc tttttcttgg caacaactgc
    43861 aaatactatc aagatgacaa ctgctcccag caagtattta ataagtccct ttttcattat
    43921 ttaagtattt atggtttatt tacttcagtt taacattctc tttgtttagc aagtaacttg
    43981 ctttcattac tttaccaaaa ctattagcta tagcttaagt ggcaatccct tgtaaaattc
    44041 cagtatcttg gttttaaaaa tcagctcata ctttgcctgt agcaaatcgg tttcggcttg
    44101 agctagcttg tttttggccg atgtaaaatc aagagctgtt actagtccaa ggttaaaccg
    44161 gttctgggtg tacctgaacg attcctccga agcagttttg cttttttgcg atgactgcaa
    44221 ttttttttcg gctgcaatag catcggcata ggcctgttga atgtccttgt ataatgcatt
    44281 tcgctcactc tccagcgcaa gctgtgcatt cataagacca attttggcgg tagttactct
    44341 gttacgagcg cttaaaccat tgaatatggg tatgcttaag cttaagccta ccgatttgct
    44401 cgcattattt ttaatctgag tcataaatgg gtcgccgtca aatgcgtaat tatcctgaat
    44461 ccggtactgt gcaccagaat tgtaatttgc ccctaacgat aaccggggtt gtaaaccacc
    44521 tttcgctatt tttaggccaa gctctgagct ttgtaaccta tacctcgcgc tctgtatttg
    44581 aggcattatt ccctcagcaa ctccaaatat ctgactgggc gattctgata ccagctttac
    44641 gctatctaat tgatgtatta caggttttgc aatctgaaat ccttcggttg tttttagctc
    44701 aaggagctgg gttaggttta ggtaggacag gtttagctgg ttttgagcat ttacaagctg
    44761 tagctcatcc gaagccacaa gtgcttctac ctcgtagagg tttccttccg gcaaactacc
    44821 agctgaaact aaaacttttg tcctttcaag ctgcattgtg gaaagttcaa cctgtctttt
    44881 agcaatctca accagctctt gctgataaag gatttgaagg taaagcgatg ctatcgttag
    44941 tgcaatgtta ttcttaacct tttcaacatc ggcaacagat gcctttaggt cgaaatggtt
    45001 ctgcttccgg gtgtttgtta gctgaaaacc attatagatg ggcaaacccg tactaactga
    45061 aaaactggtt gcctgagtat tcaaggcttc cttggtattg gtaaggtaat ttgttaccaa
    45121 gccaaagcta taggaatgat taccattgat attaattgtt ggcaatgcat ccattttgga
    45181 ttgtaaaagg tttacctcat ttacctttac gtttagctcc tgctgcttaa cctggatatt
    45241 attagctagt gcatactcaa tgcaacgctc aagtgtccac ggttcttgag aaaaaactac
    45301 cgaggttagg attaacaaaa tgaagatggg gagaatagtt tttttcatac ttaaaacgtt
    45361 atttaagaac cttacttatg acgtttattt gcggaattag tttaaacaaa aatcaatact
    45421 taaaaaattc cttttccgaa agtaggactt tataaactac catagtaaaa aagaagtgtt
    45481 aaaaaaggtt ggctactagc ttcatatagc tattggttac cttttccttt taaaaatcgg
    45541 caattgcgac tcatcaaatt ccgttttacg agggattttt attgtttcaa aatcaaaact
    45601 ttcagtatct actacaaccg cttgaaatct tccaagtgag cacgtaccac agtttaaata
    45661 tattttttta ctgttcaggt agtaaacctt atgcgattca agcttatggg tatgcccaag
    45721 tataaccata tcgtactttt ttagtagctt aagggcatac atgaggtatt tgatagtatt
    45781 gtactttcgg ggtattctgt tgacatcatc aaagtaatcg accgcgctat aaaatttgcg
    45841 ttcaacccaa cggaaacgga ctagccactt tagaaacgag tacatggtaa cgctaataaa
    45901 tcggccaaag cgtgtgccat ttaaaaaatc ggctttgtgt ccgtgctcaa tgtgtatggt
    45961 ttttcctgct gaattcttaa tatggaaatg atcatgtgta tgagggaccc aaatgtcgtg
    46021 gttgccacgg agttgtataa aacctcttcg gttgaaataa tcaataatct tagcatttac
    46081 ctccgcaatc tccgcgtagg aatacttgta gaggtcaaat atatcgccgt ttaatattac
    46141 cttctcaaca ccataatgct caattaccct atcaagtgtg gacataaagg ctttagcctt
    46201 ccacccaaag gtgccaagct ttttacctgt atcgatatgt aaatcggaaa ttacaagaat
    46261 tatcacctaa actaatttta tgctaattta agcataaaaa tcaacccata agcacctcct
    46321 taacccgttt catttcattt tccaattgct tggtggcatg catggagtac cccatgaatg
    46381 ggactaaaac tatgcggacg tttccatttt cggtatttaa atatgctatt cctcctgtac
    46441 cccaattttt ccaggttaat ccgttatgaa actttatggc cgccctatca tccgatacct
    46501 taacgtatga atagttgctt gtaaatcgct cctttaactt actaaactct ctgattgggg
    46561 tttcaacctg ttcacttaat ctatgaccgt acgcaggttc ttcaaatgag tcggactcta
    46621 tataacccag agtcttagat aggtttaacc atagtgctcc aaaaaagaga aagacaaaaa
    46681 caaaccctgc catagctgac tcgattaata ttccagaaaa ggatacgcta aactcctggt
    46741 atccattaat aataagtttt accaccaaaa tggctaaaac ataaatcaga aaagcaacca
    46801 cagcgtaata tgaaaagaaa cgtttcatct gtacaaaata tttatattat taattataac
    46861 tcgttacttt cctgctttta acaaacatta tcaagcggat ggaacaccca tagtttgact
    46921 tcgtcgagct cgcaaaaaac gctcggttgt tcatctgtgt ttgtgcaagc tgccttacat
    46981 gggtgtttca taaaatttgg tttaagtttg atctgaaaac aaaaataact cctttcactt
    47041 aaaccatcaa ccaatgcttt atttttatta caccagatag tactaacttt atatctcaat
    47101 aaaagccata aaacaattac ctttgcacaa taaaggaaga accagatgaa ggaaagtaag
    47161 gatttagttc gttggtatgc cgtttacacc aagccgcgaa acgagaaaaa ggtttacgcc
    47221 cgtttggtag aaaaggggat tgaaactttc ctccctttac agaaaaaact aaaacagtgg
    47281 agcgatagaa aaaaattggt agatgagccc cttttccgct catacatatt tgtccatatt
    47341 gacttgaaac ggtactatga tgtattaaat acccaagggg tagttcggta cataactttc
    47401 gaaggcaaag cagtccccat ccccgacagg caaattgatc agattaagca gctgcttgtt
    47461 caggatattg agcttgaagc ggtggagggt caaattgagc cgggcacaag ggttgaagtc
    47521 cgatttggct cgcttcaggg aattgaaggc gaaatggttg aacattcggg taaaaagaag
    47581 gtggttatcc ggatcgatca tatcagccac tcgctcctgg ttactctgcc tgctgagtat
    47641 gtggtggtga aaagataata aaaacagggc ttgcaccctg tttcactatc aaagtaattt
    47701 tactcaaaaa gaaaatccaa atcatcgccg gggtaaacct cgcggggtgg ttgtcctttt
    47761 ttgaaaattc gagcaggttt tgggccaatc aggctaaact gcacatcttc cagctttatc
    47821 atggttccct cgcggagtcc tacaacgtat acatgtgggt ccatttcaac gaactccata
    47881 attctttgct cacgggtttc gccagcatgt ccctcggggt tagcatccag gtagtggggg
    47941 tttatttgaa accgaaccaa actgaaagcg ctgaaactgt cgggttccgt aattggcata
    48001 tcgttagtgg tgcaaatggt aggacaggca acatttgctc ctgcactcca accaatgtaa
    48061 ggcgttccac ttaatacttt attccttact gcttcaataa tatcggtctg ctgaatagtt
    48121 tttagcaact taaaggtatt gcctccgcct acaactattg cttcggcctc ctgaaccgct
    48181 agctttgcat tccgatggtg atgtattgag tcaacctcaa ccccaaattc actgaatcgt
    48241 tcctgaacct tttgctcata ctcattgtag ctgaacgtaa cggcggccca tggaataaaa
    48301 agaacccttt taatgctacc taaaaatttt tcaatctcct tcttgggatg ctccaggtaa
    48361 gcctctcctg cattggttga gttgcttatg agtaatagtc gaaccatgat tttgaattca
    48421 ttttgtttgt ggtaaaaata caaaaaaact aataccatca aaccttaaat gcagcaataa
    48481 aagcatcctc cttaagctca agggacttta taaaattgag ggctaaatcc ttttgctctg
    48541 caaactccat caggtcaatg ctttttacca cttttcgtaa ttcattcatg gccgatttat
    48601 cgtccgatgt tccaaatagg tatacaatat cgccgggtcg aaggttcgac caatttaaag
    48661 tgctgcttac aataaaggaa ccaaattgtc gaaatgccat attatcgggg caaagagtca
    48721 atatctgctt tttatagaga ataaaaggat aaagtttgat ggaattttta cctaacgact
    48781 ttacaattgg atcatctttt aaatagagat ttaatggttc aagtacacct tcgttcaatt
    48841 ccccattatt ttttatgtac tcaataacaa tgttgtatat attgaagtta atgaaatcgc
    48901 aacaggcatt aggctcggca gcaattacat aataacagaa ggtgtaattt ttatataaaa
    48961 aatggaaaaa gtttggtttt tcatctactt ttttgatttt ggcctcccac caagcagaaa
    49021 aatatccttc aacctcgtag ctgtttttca ccatctgcag gttacaaagg ttttgggaga
    49081 tattttggaa taaatggttt cggagcagct ccagatttac taaagccctt ttcttttttc
    49141 tattttcaat taccaccaac aggaataggc taataaacac aacactaaac cccattgcta
    49201 taaataggtt taaacgttga gctttcagct tggagttcat agcgttaatt tcctgctcct
    49261 ttttatccct ttcgtattgg gcttgcagtt cttcaatttt ttgaagggtt tcagagttta
    49321 aaatggaatc cttaatggtg atatggtcaa gcaggtagct atatgctttt tgaaagttat
    49381 ttgttttaac ataacactcg gagaggaact tgttgcaatc actttcaaca tcctttaaac
    49441 caatctgctt agccttattc aacgattcct gaaacatatt tattgctcgt tcaacttttc
    49501 cttgtgcaag cagaacctgc cccatatttt tttgggtata gcataccctg gttacgcttt
    49561 taagcgattg aaacatttca agtgcattct ggtggtaaag caaagcatta tccaaaaggt
    49621 tttgtttaaa gtaaagtgaa ccgatattat cctgaattat tgctacacct attgatattc
    49681 ctaatgaatc ataaatatga agcgatttaa gaaagtactc caaggcatca tcgtactttc
    49741 caagttccaa gtatgcacta gctatattat tcgatgcccc agctactgct ctggaattgc
    49801 cctttgcctc gttaatttcc aagtattcct tgaaaaattc tattgctttt ggatagtttt
    49861 ccctgtaaaa gtgaatttgc ccaatactat tcgaagcaat tccagttccc tcaatatcac
    49921 caattttttt atataaatcc aatgcattta agtagaactc aagggcttta tcgtatttgc
    49981 cccaattacg gtaaataaga cctatgtcgt tttgtatgcc ggctaataac ctaacgctgt
    50041 cgaggtattt gtatatagtt cctgcgtttt ccaaattttc aagcgacaac cgaaactcac
    50101 ccattttcat gtagctaaag ccaatttgat gatacgcatt ggctacaagc tcccttttcc
    50161 ccaacctgtt cgctaggttt aaagcacttt tccagtaatt taaacttttc tcgtagttgc
    50221 tatcggaata ggctatggct aactcggtta gcgttttgta cctattcaaa tccgattctt
    50281 gcttttgtaa cgcttggtta agactatcaa tagaatgatt ctgtgccaaa ccattattga
    50341 atagaatggc aaaggtcagt attgccgata tgatgctaat tcttaacact aaaatcaatt
    50401 tatagcaata tacggaaaaa atgaaaacaa gtaaacagtt gtattaaaaa tgatttaata
    50461 aacgttgatg tttttgggag ctaaagaata gcaaaaagta ataagtgtta cctaatagct
    50521 taactaggta gaaaaatagt taccaatgtt gtttagttaa ggttatttgt catgttgcgc
    50581 gctagcgaaa cattatgctg tcgttctgag tcacgattta tcgggataaa cttagctgaa
    50641 gcatctcagt gctcattttt cttcaatcct tagaaaagat cactcaactt tgctcgtgat
    50701 gacaaaagca ctatcttcct ttctgctccc ttgtcatg
//
LOCUS       DAOSAL010000010        50514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_519_length_50514_cov_48.898948, whole genome shotgun sequence.
ACCESSION   DAOSAL010000010 DAOSAL010000000
VERSION     DAOSAL010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 50514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50514
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_519_length_50514_cov_48.898948"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1271)
                     /locus_tag="PK049_02660"
     CDS             complement(<1..1271)
                     /locus_tag="PK049_02660"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HOK60489.1"
                     /translation="MRARTLITILLAIAMASCGKHEPKAKYVFYFIGDGMGVSHVTLA
                     KLYADSVLLDSTAMSFTRFPVTSLSTTYAHNRYVTCSAAAGTALSTGTKTSINTIGLT
                     YDRTDTLYSIAKKFKEAGKKVAILTSVSIDHATPAAFYAHVGSRGSYYDIAKQLLKSN
                     YDFFASGGFLEPYGTKTDSNAVSVYELGKVRGCQFTSSLTEVDSLVAAGAKSIVYSAP
                     NPAPSSTLQYEIDRSESDISLADITRKAIEVVDNPNGFFIMVEGGKIDWAAHDNDAAT
                     VIHEVLAFARAIDVALEFYRKHPHETLIVVTADHETGGLSIGNRENKYDFHVKLLHYQ
                     TASEERLHKLIAEFKEQNPKAKFDDLLKFLADKTGLSKDIELNENDIKMLKDAYNVAF
                     KGLKSNEKTTYSETDPISKVSIQILNSKAAVG"
     gene            complement(1430..1903)
                     /locus_tag="PK049_02665"
     CDS             complement(1430..1903)
                     /locus_tag="PK049_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOK60490.1"
                     /translation="MAKFERKGKNELQAISTASLPDIVFMLLFFFMVSTTMRETTLMV
                     RSQLPEATEVNKLEKKSLVSYIYIGPPMRQFQSLFGTEPRIQLNDSFKSLDDIRDFIA
                     SEREAMKEDEKPLLTTSLKIDKETRMGIVTDVKQELRKASALKIVYAARKADKKK"
     gene            complement(1906..2481)
                     /locus_tag="PK049_02670"
     CDS             complement(1906..2481)
                     /locus_tag="PK049_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOK60491.1"
                     /translation="MARKVPEVNASSTADIAFLLLIFFLMTTTMNVDTGLTRMLPPMP
                     DPNQKQDDVEVKRRNIFSVLINKSDKLLVRGEPMEVGMLKEKTKEFIMNPNSDPNLSE
                     FEEKDIPGIGKFKVSKGVVSLQNDRGTSYEMYMKVQNELVAAYNELRDELSTSVYGKP
                     FSKLAKEEQETISKAIPLKISEAEPKNVGGK"
     gene            complement(2540..2971)
                     /locus_tag="PK049_02675"
     CDS             complement(2540..2971)
                     /locus_tag="PK049_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60492.1"
                     /translation="MNSSLSTYTKVLSWLLMGVSIVITILFFLSNQDIESVEPFIDWA
                     YILFAIAAVSALVFPVVNLVRNPKNVTKSLVGLLAVAVIFVIGYVLADATPIPSPTQN
                     ADLSNPTVLIISDTGLIATYLLFGISMLLLLYTGVRSAFHK"
     gene            complement(2978..3775)
                     /locus_tag="PK049_02680"
     CDS             complement(2978..3775)
                     /locus_tag="PK049_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOK60493.1"
                     /translation="MKKLFTLVAVLGVLSFGASTFVNAQDEPAAKPDSAMVQEQAAQA
                     ATPAAPAVEEQSLHKAIKTKFIEGGPGFMASILLTLIFGLAIVIERIIYLNLSTTNTK
                     KLLNAIEDALKNGGVEAAKEVCRNTRGPVASIFYQGLDRVHEGIEVVEKSIVTYGSVM
                     MGQLEKGLTWISLFIAIAPMLGFMGTVIGMISAFDAIQAAGDISPSLVAGGIKVALIT
                     TVFGLIVAIILQVFYNYLLNKIDSITNEMEDASVSLLDILVKFNLQK"
     gene            complement(3849..3939)
                     /locus_tag="PK049_02685"
     tRNA            complement(3849..3939)
                     /locus_tag="PK049_02685"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3903..3905),aa:Ser,seq:gga)
     gene            complement(4016..5053)
                     /locus_tag="PK049_02690"
     CDS             complement(4016..5053)
                     /locus_tag="PK049_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HOK60494.1"
                     /translation="MKSGNTSILIIYTGGTIGMKQNPETGALAPFNFEQIEQEVPELK
                     KFGFNLSTYSFEPPIDSSDINPDFWRKLAAIIGDNYQSYDGFVILHGTDTMSFTASAL
                     SFMLEGLSKPVVLTGSQLPIGMLRTDGKENLISAIEIAASLQNGQAMVPEVTIFFENR
                     LYRGNRTTKHNSEHFNAFRSDNYPPLAEAGISIKYNYPFIHYPTQEQHLIVHTQLDSS
                     IAVLKLFPGISENVVESILTTKGVKAVILETYGSGNAPMQKWFLKKLEYAINSNIVVL
                     NVTQCKAGGVDMDKYENGLMLKRLGVVSGHDITYEAAVAKLMFLLGKGFYGKALAHEL
                     EKSIAGEITLK"
     gene            complement(5040..5825)
                     /locus_tag="PK049_02695"
     CDS             complement(5040..5825)
                     /locus_tag="PK049_02695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOK60495.1"
                     /translation="MLLIDTHSHIFSDEFDTDREAVLMRAAEIGVHYHILPNIDSSST
                     NRLLDTCNRFPQCFPLIGLHPTSVRNGFEREIYHVEEQLAKTRFWGIGEIGIDLYWDK
                     TYFIQQQEAFRHQLRLAKKLNLPVVIHSRNSFREIFDIVDQEIDNTLKGVFHSFTGTI
                     DDYKHIDEYKTFLVGIGGIVTYKNGGVNGLVNQLDPNKILLETDSPYLSPVPVRGKRN
                     ESYNLIYIADKVAELLGVTVEQVAKQTTQNAVKLFKIKIDEIG"
     gene            complement(5829..7106)
                     /locus_tag="PK049_02700"
     CDS             complement(5829..7106)
                     /locus_tag="PK049_02700"
                     /inference="COORDINATES: protein motif:HMM:NF039309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOK60496.1"
                     /translation="MLKKILPLLVISLLTVFIIDYFIGTAYIKLYSPEKDQKPLVEDV
                     QPILEYGIPIDSFYVTEYTVRSNQTLGKILFSLNLTGNVNEFLLSLTPDIFDVKKVRA
                     GARYKVFARKDSLQTPAYLVYEVSPVEYFVFNLTDSLYVYPFKKEIVPVRKISEAEIK
                     SSLWVDMKANGLNPMLALELSDIFAWTVDFFGLFKGDKFRVMYDELYVDSTSIGIGTV
                     YAAWFEHRGEKFYAFRFEQDSIASYWDEKGNSLRKSFLKAPLRFSRISSRFSGNRFHP
                     VLKIYRPHSGVDYAAPEGTPVMALGDGVVIDMGYDKAAGNYIKIRHNSVYTTGYNHFS
                     RFAKGIARGARVKQGDIIGYVGRTGYATGPHLDMRVWMNGSPIDPLKMKSPPVEPVKQ
                     ENLETFRNVVAKLSAQLDSITPGSRIYYAVQKE"
     gene            7202..8248
                     /gene="thiL"
                     /locus_tag="PK049_02705"
     CDS             7202..8248
                     /gene="thiL"
                     /locus_tag="PK049_02705"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HOK60497.1"
                     /translation="MSETQKRTELYELGEFKLIDRLTEPFAPKHQETLKGVGDDAAVI
                     SCGKGECLVVTTDLLLEGIHFDLTYTPLKHLGYKAIVVNLSDVYAMNAIPKQVTVSIG
                     VSKRFCVEDLDELYSGIKLACDSYNVDLVGGDTSSSMTGMTISVTAIGSAKEDEIVYR
                     SGAKVNDLICVTGNLGGAYMGLQLLEREKRIFLSNKAAKPKLEGYDYILERFLKPEVR
                     RNVIENLRKAGVKPTSMIDVSDGLSSELLHICKNSNVGCRIYMDKIPIDEQTARMAEE
                     FNIDMFTAALNGGEDYELLFTVPLSEYQVVDSMENVRVIGHIVEPHLGAYMIPPSGED
                     IRITAQGWNALQKE"
     gene            complement(8286..8669)
                     /gene="gcvH"
                     /locus_tag="PK049_02710"
     CDS             complement(8286..8669)
                     /gene="gcvH"
                     /locus_tag="PK049_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOK60498.1"
                     /translation="MNIPSNLKYTKDHEWIKDEGNGMATVGVTDFAQTQLGDIVFIEI
                     ETQGETLAREEVFGTIEAVKTVSDMFMPVSGEVVEVNPKVTEQPDIVNKDPYGEGWMI
                     KIKMSNPAEMNDLLNAEQYKGIINE"
     gene            complement(8788..16140)
                     /gene="sprA"
                     /locus_tag="PK049_02715"
     CDS             complement(8788..16140)
                     /gene="sprA"
                     /locus_tag="PK049_02715"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HOK60499.1"
                     /translation="MRVKHLSHTHTIRIIGILTLVTITITRAFSVPIPQQDTLNLPFP
                     IKQPGIWSIDTQYSPLYLSSPSNISEEVVFDPINNQYIIYRKVGNTTIETPRVLSADE
                     YRSYRVEKAMREYWRQKQTGEFVGKGDGILPRIQVGGETFDRIFGSNTIEIIPQGNAE
                     LVFGVSSAKTDNPALPIDQRRNTTFDFQSKIQMNVSGKIGEKLKMEVNYNTEATFDFE
                     NNVKIEYNGFEDEIIQRIEAGNVSLPLPGTLITGSQSLFGIKTQLKFGKLNVTSVVSK
                     QNGQTQVVEIKGGAQTRDFQLKADEYDANRHFFLSHYFRENYNRALMNLPIINSGVQI
                     TKIEVWVTNKQANFENSRNIIAFADLGEAQNNIFASNIFAQTGSGPASNGLNNLYELM
                     TTTYAGIRDVAEVSNVLLPLESQGFTGGRDYEKIEYARKLSPNEFTINQALGYISLSS
                     ALNSDEVLAVAYEYTYNGQTYKVGEFSTDGVEAPKALILKLLKGTNLSPKMPTWRLMM
                     KNIYSMNAYQISKDDFRLDIFYQDDATGTPINYIKEGNIAEVPLIRVLNLDNLNNQLD
                     AFPDGVFDFIEGVTINPANGKIIFPVLEPFGRDLASRIGDPTIAQKYIFQELYDSSLT
                     KARQVADKNKFLIGGSYKSAGGSEIFLNAPNIPKGAVVVTAGGVKLQEDVDFIVDYNL
                     GTVRIINQGLLESGTPIKVAVESNALFSLQTKTLVGTHFDYRFSDNFNVGATIMNLTE
                     RPLTQKVSFGNEAVSNTIWGFNTSYQTESKFLTKMVDFLPFIQTKEKSTITFDAEFAQ
                     LIPGHSRAIGKAGTVYIDDFEGSSTSLDIRSWVEWKLASTPQGQPDMFPEASFSNDLR
                     YGYNRAHLAWYYIDPLFLRNTSLTPSYLRNNKELQGNHFVREIFERELFPNKNTPQGQ
                     PTNISVLNLAFYPTERGQYNFNTTELDANGRLIDPQRRWGGIMRNLSTTDFEASNIEY
                     IEFWLMDPFVYDTLSSGGDFYINLGNISEDILRDGRKSFENGLPTSSEELSKTDQTAW
                     GRVPTKQYLPVGFDNDQAKRPYQDIGLDGLSSSVDLDGDGILDEQFFFSEYINQLRNK
                     ITNSDVLAGFESDPAGDNFRHYLSGVYDQQRATILDRYKYFNGTENNSPVATGTTSQQ
                     SYSTPDVEELGTDNTMNENEAYYQYRISLRPQDMEVGKNFITNVVTGDKDKILNKAVK
                     WYQFKVPIAEYEKVVGPIEDFKSIRFMRMFLRGFQDTVILRFATLHLVRSEWRKYNYS
                     LIQGQEGMPVGDLPSGSLSIATVNIEENDKRTPVNYVLPPGVDRVIDPSQPQLIQLNE
                     QAMEYRVTDLADGDARAAFKNVNIDIRQYKRLKMDVHAEAIEGYPLNDYEVNAFIRIG
                     TDYNTNYYEYEIPLKLTPYGFYYNSRERDREAVWPRENVIDIDLDKLVDVKVARNNAM
                     QKPGSLITLNTVYVVQSGNSKLKVTGNPNLSNVKVIMIGIRNPADDRMPKSVIAWFNE
                     LRLTNFNDQSGWAANARLSAKLADLGMVTVAGTTITPGFGSIEKKVNERSKETVYQYD
                     INSTLELGKFFPASFNIKIPMFIGFGESMANPQYNPLDPDILLDDALRNASERERDSI
                     KRIAQDYTRRRSINFTNVKIDKMGSKPRLWDPANISVSYSYSEIFSRNVRIDHRIQRN
                     IRGAFTYNYNRQPKYISPFSKIKWLNSSYLALIRDFNFNLFPTQLSFRTDLNRNYFEQ
                     QFRNIQNPNQVFLPTFSKDFMWNRNYSLNWDITQSVKFDFVATATARIDEPEGMVDRY
                     RDRDRYEQWKDSVWQNIRRGGRNTMYNHQFNITYSLPLSKIPLLNWISITSRYTGIYR
                     WQAGPILADTSRFDPGNTVQNSNTIQLNGQVNINTLFNKVGYLKRVNQQFDQRARGAA
                     PQTRTVKYEEEKVNLRANATKVITHKLKTEKVTVKVFDPNGKEIKVSSQVRGDTKVAI
                     KADADYKNCKVVVEGKVPEKINPAKFALETTLRLLMSVKSMGASYTETNGTLLPGYKP
                     GVNYLGTENFNGMLAPGWEFVAGWQDPDFAWKAATNGWLTRDTSFNTPVVFTRNENLN
                     FRATLEPLPGFRFDVSATRTLGKSNSKIFKSDGLGSYRVLSPLTTGNFSISVLTIGTA
                     FEGGKRTGSSKAFERMKQNRLEIAYRLRDRRVANPSQGYDPNVIDPVTGFPDGYGPLS
                     QDVLRASFLAAYTNTSPSKVSLSDFPLIPMPNWQLSYDGLAKMKPFKRWFKSITINHG
                     YRSLYTIGAFTSNLTYLPEDDGFSYVRNINGDFVPQNEITNVAINEQFTPLVGLDLGW
                     NNNLTNRIEIKNSRSLAMSFANNQLTEIYGNEFIVGAGYRFEDLPLIFQAEDGGKKAI
                     KSDLRLTVDFSLRDNTTILRKLVEDSNDIIAGQLISTIKTAADYRINEKVTIRLFFDR
                     TLNNPKVSRTFRTTNTSFGFSVRFELVN"
     gene            complement(16163..16747)
                     /gene="ruvA"
                     /locus_tag="PK049_02720"
     CDS             complement(16163..16747)
                     /gene="ruvA"
                     /locus_tag="PK049_02720"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010539156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOK60500.1"
                     /translation="MYEYIQGSLAELTPTHAIVDAGGVGYFVNISLQTYSQLEGKTQV
                     KLWLHQIIREDAHLLYGFTDVREREIFRLLISVSGVGASTARLILSSLTTDEIQAAIT
                     TENVNLIKQVKGIGLKTAQRIVVDLKDKVSIGEATVSQILSTSNNTLKSEALSALVML
                     GFARSAADKVIDQVLKEMGQMPVEALIKHALKRL"
     gene            complement(16760..17812)
                     /gene="pdxA"
                     /locus_tag="PK049_02725"
     CDS             complement(16760..17812)
                     /gene="pdxA"
                     /locus_tag="PK049_02725"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOK60501.1"
                     /translation="MSNEKIRVGITQGDINGIGYEVIIKTLMDPRMDEMCTSIVYGSP
                     KVAAYHRKALNIENFSFNQIKDASEANPKRPNIVNCMDENVRVELGKSTEYGGQGALL
                     ALQAAIADLKAGKIDVLVTAPINKANIQSQSFSFPGHTEFLANEFGVKSALMLMVSDT
                     MKVGVVTGHIPIADVPKNITVDAILNKLRLLNETLIKDFAIRRPRIAVLGLNPHAGDS
                     GVIGNEEETIILPAIKQANEEGIVAMGPYPADGFFGSDRFTRFDAILAMYHDQGLSPF
                     KVINFDTGVNYTAGLPIVRTSPDHGTAYDLAGHNLANPNSFRQAVYLAIDIFRNRQLH
                     KELTSNPLQSVDPETA"
     gene            17945..19099
                     /locus_tag="PK049_02730"
     CDS             17945..19099
                     /locus_tag="PK049_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HOK60502.1"
                     /translation="MQVKDILKKAINLESLTLEEGLYIYTQASTPDLMYVANELRKIH
                     VPGHKVTWQIDRNVNITNVCISGCKFCNFHCGLQSDSAFITSIAEYKVKIEELERYGG
                     DQLLLQGGLHPRLGVEFYCSLFKELKSMFPNIKLHALGPPEIAFIAKKEGMTYRQVLE
                     ELVEAGLDSLPGAGAEILVDRVRKQISPGKPDSQAWLDVMAQAHQMGLVTSATMMIGH
                     VETLEERVEHLIKLRNLQAQKPADAPGFLNFIVWTFQDEGTKLQREGVLNTVTTEEYV
                     RTIALCRIMLNNIPNIQASWLTVGIDTAQLCLHAGANDFGSIMIEENVVSSAGAKYKM
                     DAYGIQKAIVDAGFEPQLRNQRYEYIVLSDCEFSRIYSIEELKQNTSPLL"
     gene            complement(19162..19668)
                     /locus_tag="PK049_02735"
     CDS             complement(19162..19668)
                     /locus_tag="PK049_02735"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOK60503.1"
                     /translation="MRKITAFFPVLLVAAFALGACNGTAAKQNEGEPVTALKPAKEKY
                     AIDITRDFFLTKILDYTKGQENMKYLGDKPAIVDFWASWCGPCRIAGPILEELAKEYD
                     GKIYVYKVNTQNEQQISAELGIQSIPTFIFFPMDGKPFATSGIAQTPEETKKMFKEII
                     EKELLKKK"
     gene            complement(19727..19927)
                     /locus_tag="PK049_02740"
     CDS             complement(19727..19927)
                     /locus_tag="PK049_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOK60504.1"
                     /translation="MSTIVVDEARCPQNHPCPVVRYCPTGAIKQRSWNSAPEIDKENC
                     KNCGLCVRICGYGAFTFADNGR"
     gene            complement(20139..20966)
                     /locus_tag="PK049_02745"
     CDS             complement(20139..20966)
                     /locus_tag="PK049_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60505.1"
                     /translation="MKRTSLIFAAGLMLMAAFTLSSCQKDEEVDSVVTLASEDADVDT
                     YYDDVMTEVDEITLAGDSKDSQLMYATLGGQGTRTRKTSWDGNCRIDSLTYENFVNAN
                     SRYERVKNGLIVIRTCGQYNGAEFERTVTFKNFTINGNLIEGTKQIRKTAEHEYQITL
                     TNGKITFTDGTTYTRTVERTRTWVEGYDTPFYIWDDVYTLEGVATGVNRRGYTYTHQI
                     TSALMFKLSCRWLVQGVIDLTVGDKQATIDYGNGDCDNIAYVTVNGKTYEVKLRGGK"
     gene            complement(21008..21883)
                     /locus_tag="PK049_02750"
     CDS             complement(21008..21883)
                     /locus_tag="PK049_02750"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOK60506.1"
                     /translation="MTRDGKIERLPENIERLLDSYNVPAGKMRSEAWEAIFEKIDHKN
                     FEEDSKGRVIQLTLTRVAVAASVLVALMLTSYIYLYRMGNVHVQLARGEHGVVYLPDS
                     SRVTLNAESKITYNKNRWFFDRSVSLTGEALFEVSKGRRFKVVTTGATTTVLGTVFNV
                     FSRNGLVKVTCFEGKVKVVATNSKSQTLLTKGMEAKTMGYALDVNKTQTTKAESKPAW
                     TNNEFFFQNAPLSAVIEELERQFNVDIFLNTDTSRFYTGYFKRTNLDEALNLVCIPLD
                     LKWDYSGEIIVITSK"
     gene            22161..22964
                     /locus_tag="PK049_02755"
     CDS             22161..22964
                     /locus_tag="PK049_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60507.1"
                     /translation="MENKVLFTDSVVANFCKATVDDNPIHDPVFMYNQNKGVVVPGML
                     LFSTIVNLLHRKTDEYFNYYKLIFGNVICTNEPIDLGFETKGDQNYLYAINGHDMFST
                     KNERSVVLKNIEPKVLDLDGIVRAMPFHKTQLETFRELTNSLNNTLSDFLFTIAYASA
                     ALFKAIREPITEVETEINRLLNKAINPERVSPFYQTLEIFRVNPHAELTPEGTIEYNI
                     RFNREKANKIYVAQVKCMHNSQCFYQSVYRLVAIPDKLIMRMVKDRDKV"
     gene            complement(23057..25708)
                     /locus_tag="PK049_02760"
     CDS             complement(23057..25708)
                     /locus_tag="PK049_02760"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOK60508.1"
                     /translation="MSRIKYLLISLLVGFALQIQQATYAQKVKITESFSNQSLDQILS
                     ALSGKYNIKFAYDPVALNQISFTGQFRNDSPDKVLKQLLKDSGFEFIALNNVYVIKRA
                     EKPIVEEKNKPIQGIVRDRISGEALPYASIRVVDNDLEATTNTDGFFTIPDVKSDSVS
                     IEVRYLGFEPLQMRVARVQYNSKPIVVELDPKKFILADVEVVRNITSMLATGDRPGEM
                     VFDTKKVGDIPGISGIDILAPLQLLPGVDGTTESLSGLMVRHSPADKNLISYDGFTIY
                     HINHLFGAFSSLNSKAIKDVRLYRGGFDSRWGGRASSVIEITGKTGNENSVSVDAGTD
                     QLAGDFTFEGPLGKHASFIVSARRSYTDFYRSPQFLNLFESVASDLMLSKQNFTAFGS
                     DPDAPSYLFYDANAKVSVKPSNNDILSVSGYVGRDRLNFLQMVSSPFINENALWGNSG
                     AGARWAKQWNSWLYQNFTVGISEFTSNYQHYDSTLKRRGIQPVYDTIVRNYATETGLN
                     DLSVNLNAQVTLSNQIILELGYQSNLVNSKSSENYYHAVNSLPVADTLRQYDFNSRVN
                     TGWLQFNFKTNGLKSLVIGGRVSQHNLTDQYYVEPRVQMVISPTSKLDLKFAAGLYNQ
                     FINRIYLTGNGVRYLWVASDEKTFPVVKSKHFVTGLNYTSNGLTLDIEGYAKQTEGLS
                     YVQTVIRRTSTNRFVEQSKIFYVDSRSLGVDVLVRKTWQNAEGWVSYSLSKAFNQSSN
                     LNGGEEYYALDDHLHELKLVGLYKWRKWRVVGTWIFGTPKPWDELLLTPSLTLSPDYE
                     KNSSRLEPYHRLDAGLSYLAKVSVSGELEVGVKVFNLYNRDNMLARPYTLTDTPISDY
                     LQGKSIIEYHDIYGYSITPTFFFNIKF"
     gene            complement(25762..26643)
                     /locus_tag="PK049_02765"
     CDS             complement(25762..26643)
                     /locus_tag="PK049_02765"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HOK60509.1"
                     /translation="MKRDRNINSTDSGFDSNPIEFNISVPPSNRTKEQAWDMLMQTIE
                     GKHAEPARVVTFQPHYKWLLAAASVIILMLVGSLFILNTESRFETMQAQTLEHTLPDG
                     SVVHLNASSWISYKKSYGWFGRNVRMEGEAYFKVKSGKGKFVVTDNQKQKVVVTGTEF
                     SLVTRGDSFEVRCFEGSVNVLISGLKPVGVKKGESVTKTNGMLIHSKQSDKELTKPVW
                     VTHEFEFAEEPLSQVFQQISNFYNIQISAEGFDPASRYFSGTIPAGKATDVLDVISIS
                     MGLSYTVNADSTVYTFR"
     gene            complement(26640..27167)
                     /locus_tag="PK049_02770"
     CDS             complement(26640..27167)
                     /locus_tag="PK049_02770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HOK60510.1"
                     /translation="MTLPSGNYTKEELSWFRSIFDQYYESIRHFAYYKTGDVDLSDDI
                     VQETFLKLWDMRGKVNNDTVKSLLYTIAGNIIKNHYKHLKVVYSFQVAVKGDMTSNPN
                     SADERIRMDQLNKQLQEVLASLPDGQREVFLMNRIDGLTYEEIAARLGLSVKAIEKRM
                     SEALAAIRQKFGYKI"
     gene            complement(27285..29090)
                     /gene="rho"
                     /locus_tag="PK049_02775"
     CDS             complement(27285..29090)
                     /gene="rho"
                     /locus_tag="PK049_02775"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOK60511.1"
                     /translation="MYDILELNKKLLPELREIAKNLNIKKVENFKKQELIYKILDQQA
                     INASLSADDERKKDHRKPFRKPEKQVEVKREETQPVAPKKEEPQAAKPDEATSSQEAI
                     NERNKRKRIIRAKEVRKEFSATPTNSSSEEKERSNKTTIKFNEKAVILPELDTETHSE
                     TILLSNDEEDTVIPMVTEEAQAEALVSEKPEQEARASVTEVVEPVKEENNAQPIPSQE
                     RRPGSERFDFDGIVATTGVLEIMPDGYGFLRSSDYNYLSSPDDVYVSQSQIKLFGLKT
                     GDTVTGTIRPPKEGEKYFPLIKVIEINGRDPEFIRDRVPFDYLTPLFPNEKFKLIGNG
                     MNLLSARIVDMFAPIGKGQRGLIVAQPKTGKTILLKDIANSIAANHPEVYMIVLLIDE
                     RPEEVTDMARSVNAEVIASTFDEPAERHVRVANIVLEKAKRLVECGHDVVILLDSITR
                     LARAFNTVQPASGKVLSGGVDANALQKPKRFFGAARNIENGGSLTILATALTETGSKM
                     DDVIFEEFKGTGNMELQLDRKLANKRVFPAVDIMASSTRREDLLLDKEVLQKIWILRN
                     YLTDMNSVEAMEFLRDRLVKTATNEEFLWSMNSDF"
     gene            29471..30742
                     /locus_tag="PK049_02780"
     CDS             29471..30742
                     /locus_tag="PK049_02780"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOK60512.1"
                     /translation="MKTLNALIGKSLRYSATAVALIISAVLTAQEVDYNLVNGVIKDA
                     KTKERVVFATISVPGTTIGTVANADGEFTLKVPKTVKSNVFEITHLGYQNTQFPIVNG
                     TSGKDKVYLIEPSSLEIQSVVIRPMDARTILEGALKNIDKNYSDKPVSFTAFYRESIK
                     QRREYISIAEAVAEIYKAPYRYTLETDKVKIVKGRRGENVKKADTLMVKMQGGPHVSL
                     YLDVIKNPELILTREDLPYYNYEVVDVVKIDNEANYVISFTPNTKLEYPLYQGKLYIN
                     TENLAITMAEFSIDLSEPEKVAQIFVRKKPAGLIFEPISTNYLVTYKKVNDKYHVNYM
                     RSEVKFRADWRKRIFKTNYTIMSEMAITERNFDNVNKITFKESFKPNLILADRVNEYF
                     DENYWGSYNTIEPDESIQSAISKFNKRFRKQ"
     gene            30824..31351
                     /locus_tag="PK049_02785"
     CDS             30824..31351
                     /locus_tag="PK049_02785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HOK60513.1"
                     /translation="MVLASTNPNEPDLKEFEKVFRKYYEPLCRYAYSIVRDADEAEEI
                     VQEFFYTFWLNRNRISIRLSLKSYLYRSIRNSALRYLQHKNIEDQYRTQDYALNHVES
                     PEQVIQGNEMAGIIDKTLKGLPERTRQIFLLNRINGLKYKEIADLLKISIKTVEADMG
                     KALLELRLQLKRAGF"
     gene            31379..32362
                     /locus_tag="PK049_02790"
     CDS             31379..32362
                     /locus_tag="PK049_02790"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOK60514.1"
                     /translation="MNSKKSYQTHIAKYLSNEMALAAKIWFYLKNQINPKTKHELNTL
                     KVFFNSMKVNEKNKDIEFDTGKAWQKLQERIKTESYTGQSRTAFIPNLNYFAVAATLL
                     VMVALSLSVWFYYNPKTITLANMTQSSTIIRTLPDGTQIFLGENTILEYPVRFAGKTR
                     TVKLSGEAYFDVAKNGSKPFIIKTSHADVKVVGTSFNLKTSNNEVHLKVTEGKVKITL
                     ATNQKSALVQAGEAAIASKNDIVTYTQPAGETIKSAMKLLMFQDETLDNIVRVINSTY
                     GSNIKVLGDDLKDMRISVTFDNDISSIVNILSVSFNLKVTRSPDGTIIISR"
     gene            32337..>33832
                     /locus_tag="PK049_02795"
     CDS             32337..>33832
                     /locus_tag="PK049_02795"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOK60515.1"
                     /translation="MEPSSLVGKNIKVKPFWHVFHWVVLFMLIIYAVKANAYEPDSLL
                     RQNISISIRTTTLFKALNQIGDKAGCFFIYDSRDVESNRRVKSIEWSDKPLNDLLNQV
                     IGDTLVKYRVIGKHILLYRPKLSDTLKAQSQEQNIKYVQLSGRILDKETAQPVAFATV
                     GIPSLAIGTISNLDGVFTLRIPKQNTDSSIAISHIGYIQRKVPAGLLTAKTFEIFLEP
                     SFIPVQEIFIRNIDARNTVREAVNKRSQNYFQQDIYITAFYREGVLRNKRILSYSEAF
                     AKIFKSSYSFDSRYDQVKLIRFRKMEQANRRDTLDVKLQAGVKATLDIDIMKNLPDFL
                     DPEFTQYYDYTRTDIVVYDNKSAYAIGFKQKPEITDPLFAGLLYIDTETLTLLAAEFE
                     INPDMVQKTASQLVVKRGRGIRVQPQKIKYLVKYQPHDGKWYVNHVRGEISLKVQTRF
                     WPFANTFDLFFEYVALQVETLEVKRFPKRETLKTTEVFSNTQAEYDPN"
     assembly_gap    33833..33932
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(34078..34620)
                     /locus_tag="PK049_02800"
     CDS             complement(34078..34620)
                     /locus_tag="PK049_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60516.1"
                     /translation="MKKTILISVFVAINMMSLGQVFNTGQTLKPKTFSVGIEPAVLIN
                     GSNEFMLFLHGGYGLTKGIDVGITAGVLGPENYFGANVEFVLSKNISLAGGAHSFGSF
                     GLDGTLNLTFGLAKGVRLITGLDTDINFPDGGDAQFLLWIPAGVDIGLRKNMSFILEA
                     EIGLTDPAYNLIGGGVVFYF"
     gene            34828..35376
                     /locus_tag="PK049_02805"
     CDS             34828..35376
                     /locus_tag="PK049_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60517.1"
                     /translation="MNVEVKTTAGQAMGIVGIVLAVISLILAFIPCIGFVALLPAALA
                     LIFSIISIIQATNGYGSKGLGIGALIVSVLSIFLAVMWLTIISGGFSVLKDLERHPEK
                     IEIFGKELEKELNDANVQIRIETDSMVKTLRQLEGESDSIKVITIKKNKVSKGDDSQG
                     ARITIDTDSVQIRIEATEKKKK"
     gene            35348..35809
                     /locus_tag="PK049_02810"
     CDS             35348..35809
                     /locus_tag="PK049_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60518.1"
                     /translation="MKPLKKRKSNGFNFYSIAATPYLKVNSAFALILLLVFIYSLVFS
                     QNNHPIPAALTQLTGIVPPSKGLSAAFSQIVRLNFTNAYILNPYSLRVFSFFLIQFIL
                     RVGLIVTELRFPNSVTTSTIAVDILISLTLLIYCFYPLIEFTFKILLQLLH"
     gene            complement(35789..>36354)
                     /locus_tag="PK049_02815"
     CDS             complement(35789..>36354)
                     /locus_tag="PK049_02815"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOK60519.1"
                     /translation="DVVLTHLHYDHCGGGVYRDSDGNLKLTFPNARYHVSRAQWEWAV
                     NPNPREADAYLPENLLPMRDLGALNLIEDEGELLPGIELRLFNGHTRGQIIPIISYGG
                     RKLVFVADLFPFRSHIPLPWIMSYDVEPLVTLKEKETFLKESLEGEYIYIYQHDYFAD
                     CSELQLTPKGIRATDGFTFEELMEKLK"
     assembly_gap    36355..36454
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<36455..36795)
                     /locus_tag="PK049_02820"
     CDS             complement(<36455..36795)
                     /locus_tag="PK049_02820"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOK60520.1"
                     /translation="MKLSLFNISNFKVDGGAMFGVVPKVLWSRVYPPDDNNLIPLSLK
                     SLIVEVNGRVVLFDNGIGNKQDEKFMSHVYAFGGEGLIEGLGQRGYKPTDITDVVLTH
                     LHYDHCGGGVYR"
     gene            36932..38155
                     /gene="clpX"
                     /locus_tag="PK049_02825"
     CDS             36932..38155
                     /gene="clpX"
                     /locus_tag="PK049_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004294102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOK60521.1"
                     /translation="MKNTDKCSFCNRSRKEVGLLIAGLDGFICDLCAEQAYLIVKEEL
                     KKKGEFKLQKNKLLKPLEIKKFIDQYVIGQNEAKKYLSVAVYNHYKRLLQADGGDDVE
                     IEKSNILLVGETGTGKTLLARTIARMLHVPFTIVDATVLTEAGYVGEDVESILTRLLQ
                     VADYNVEAAEKGIVFIDEIDKIARKGDNPSITRDVSGEGVQQALLKLLEGSIVNVPPQ
                     GGRKHPEQKMIAVDTKNILFICGGAFEGIQKKIAQRLNTTVVGYGASKTREVIDKDNL
                     LQYIAPQDLRAYGLIPEIIGRLPVITYLNPLDRKALRDILTEPKNAIIKQYIKLFEYD
                     GIHLKFDAEVLDYIVDKAVEFKLGARGLRSICEAIMIDAMFELPTSEQKEMVVTLEYA
                     QNKLEKSNLKRLKAA"
     gene            complement(38152..38637)
                     /locus_tag="PK049_02830"
     CDS             complement(38152..38637)
                     /locus_tag="PK049_02830"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOK60522.1"
                     /translation="MKISIIVAIAENGVIGGGNELLWHIPDDLKRFKRITSGHSVIMG
                     RKTFESIGKPLPKRRNIVITRNSEFRVDGIEVANSLRTALEMTKSEDEVFVVGGGEVY
                     RQALPLANKLYITRVHDKYEGDTFFPDIDMNDWQPVSSEKGNPTTGPGYTFEEYVRIK
                     H"
     gene            complement(38652..>39070)
                     /gene="thyA"
                     /locus_tag="PK049_02835"
     CDS             complement(38652..>39070)
                     /gene="thyA"
                     /locus_tag="PK049_02835"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008629867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOK60523.1"
                     /translation="RHIVSAWNVGEIENMALPPCHVLFQFYVAHGKLSCQLYQRSADV
                     FLGVPFNIASYALLTMMMAQVCGYEPGEFVHTLGDTHIYLNHIEQVKLQLTREPRKLP
                     RMVINPEVKSIFDFKFADFKLEDYNPHPHIKGDIAV"
     assembly_gap    39071..39170
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<39171..39601)
                     /gene="thyA"
                     /locus_tag="PK049_02840"
     CDS             complement(<39171..39601)
                     /gene="thyA"
                     /locus_tag="PK049_02840"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009229476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOK60524.1"
                     /translation="MVQYLNLLDHILNHGVRKDDRTGTGTISVFGYQMRFNLQDGFPL
                     LTTKKLHLKSIIHELLWFLKGETNIRYLNENGVTIWDEWANANGELGPVYGYQWRSWP
                     TADGRHIDQISKVVDSLKNNPDSRRHIVSAWNVGEIENMALP"
     gene            complement(39664..40644)
                     /locus_tag="PK049_02845"
     CDS             complement(39664..40644)
                     /locus_tag="PK049_02845"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOK60525.1"
                     /translation="MRSKLLAIAVSLRFLIGSISERILECFKRAFPKALKTSFWILKI
                     TLPISFLVSVLSYFGLLNAFSELLTPAFKLIGLRGEAAIAFVTGVFLNIYSVIVVLSQ
                     LTFTLREVTIIAVMSLIAHNLIVETAVQSQTGSKAWHMVILRLGMAFVAAFLLNLTLP
                     PLDQAFGHPANHEQTLSLVAHLHGWLFSAFRMIFKIIVLIFLLMVLQEMLREFNLIEV
                     LTAPVSPVIRFLGLPQSTTFLWIIANTLGLAYGSAVMLDEVSQGRIRKAESDLLNYHI
                     AISHSNLEDLLLFAALGVSISWMLGIRLIIAALAVWARRWYLSAKGEVFI"
     gene            complement(40722..40803)
                     /locus_tag="PK049_02850"
     tRNA            complement(40722..40803)
                     /locus_tag="PK049_02850"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40767..40769),aa:Leu,seq:tag)
     gene            complement(40869..41594)
                     /gene="lptB"
                     /locus_tag="PK049_02855"
     CDS             complement(40869..41594)
                     /gene="lptB"
                     /locus_tag="PK049_02855"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010265847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOK60526.1"
                     /translation="MRLYTQNLVKRYKTRTVVKGVSVEVSQGEIVGLLGPNGAGKTTT
                     FYMIVGLIKPNSGRIFLDDLDLTDEPVFRRAQRGIGYLAQEASVFRQLSVEDNIKAVL
                     EMTNFSKEYQHEKLESLLNEFGLQKIRKSLGIQLSGGERRRTEIARALALDPKFILLD
                     EPFAGVDPIAVEDIQNIVAHLKDRNIGILITDHNVHETLSITDRAYLLYDGEILKAGT
                     AEELADDEQVRKLYLGQNFELRK"
     assembly_gap    41813..41912
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <41913..43845
                     /locus_tag="PK049_02860"
     CDS             <41913..43845
                     /locus_tag="PK049_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HOK60527.1"
                     /translation="VTLHKKFSKMINPPKHTPRWIVFLIDLVICLFSITFSFFVRFEF
                     SISEEYRILLKSVIPLVLFIRAVHFLSFRTFAGLIRYTSTKDAERIFVVTSVSTFVLF
                     VINFINFFISSRFIIPNSILLIDYFLTIILMVGSRLFYKTLYYTIRNSDLNSEKILIV
                     GVEQFAAAVKRAIDRESLTGIQIVGFIDPYNKQEGNKIENIEIYNIGKIEYLIQKHDV
                     AKVILATKNLDPNRKNKIIEKCLNLNVSVQTIPDANAWINGELNVKQIKNIKIEDLLE
                     RPPIVLDKKRIQDYIKGKVVLVTGAAGSIGSEMVRQITRFRPNKIILFDVAETPLYEL
                     ELELKETFQFFDFETVIGSVTNEYRVNRLFEAFKPQVVFHAAAYKHVPMMENNPTEAV
                     FNNVLGTKLVADIAVKFHVEKFVMISTDKAVNPTNVMGASKRIAEIYTQTLNYTSQTK
                     FITTRFGNVLGSNGSVIPRFKKQIENGGPITITHPDIIRYFMTIPEACQLVLEAGAMG
                     QGGEIFIFDMGKPVKILDLAKKMIKLSGLTYGKDIQISFTGLRPGEKLFEELLNNKEN
                     TIPTYHPKIMIAKVNEYKQEEVMPKIMELLNTLPSHNNFRIVSIMKDIVPEFVSRNSI
                     FEEIDQQKKYRNEQQSKTA"
     gene            43820..44260
                     /locus_tag="PK049_02865"
     CDS             43820..44260
                     /locus_tag="PK049_02865"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOK60528.1"
                     /translation="MSSNPKLHKFFHRTPIQIRFNDIDQLSHVTNSVYQQYFDLGRLK
                     YFTEVLQEKMNWEIEGLVLASITIDYLIPIRMWDNIEVLSKVYEIGNKSLKMRQEIFN
                     NTTGEVAAVSKAVMVCFSNAAGKSIPIPHRWRELILSFEKDLQL"
     gene            complement(44255..44776)
                     /gene="rimM"
                     /locus_tag="PK049_02870"
     CDS             complement(44255..44776)
                     /gene="rimM"
                     /locus_tag="PK049_02870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOK60529.1"
                     /translation="MGDAGFVEIGYIQRTHGINGELAVSLNSSVEFNPEELESVFLEI
                     EGIPVPFFITRIRFQNPEKAIVKFDDVDSIDQAQELYGVKMLIPSTSIELEDEVYLSD
                     LVGYKVRNTDKSEVGVIVDYTEYSMNATFELVTPDGKHVLIPAADELIVEVDTSAKLL
                     EMELPEGLIDLNL"
     gene            complement(44788..45309)
                     /locus_tag="PK049_02875"
     CDS             complement(44788..45309)
                     /locus_tag="PK049_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOK60530.1"
                     /translation="MPVKIRLSRQGRKKRPFYHIVVADSRAPRDGRFIERIGSYNPLT
                     NPATIELNFDRALDWLQKGAQPTDTCRAILSYKGVMLKKHLLEGVKKGALTPEQAEAK
                     FQDWVSQKEAKIKAKIDSLAKGEAEEMKKRMEAESKVRDARAQEIAKKRAEMAAKEAD
                     SQAESTSEETENA"
     gene            45498..46874
                     /locus_tag="PK049_02880"
     CDS             45498..46874
                     /locus_tag="PK049_02880"
                     /inference="COORDINATES: protein motif:HMM:NF024981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HOK60531.1"
                     /translation="MKRVSTITQKLLVFLLVILLSSLANAETIAKKIKTIPQGSDSAF
                     VTKTLGSPTEKQVVSRYFYQGNQAVFIDSVIRDIRLAEPHKKLKIKLKRQLKAEQKKE
                     TPISSLRIGMNISEALERAGEPDSIVKGEDWYYTNRHRIEIANGKVIKTEVHLKATLE
                     TLDWIWLNFTKGGLLFMNITLALIMFGIALQIKIEHFKLLLVSPKPVILGFLSQFLAL
                     PLFTFLLVLIIQPTPSVAMGMILVAACPGGNISNFISSLAKGNVALSITLTAIATLAA
                     IFMTPFNFAFWGKLYSAASNLVIPIEIDAWEMIRTVLILLGIPVILGIAFARFFPNTA
                     ERISKPFKIFSIVAFLGFVVAALAANFGYFLKYIHLIALIVIVHNGLGLLVGYTIPSI
                     FKLSKQDRRTIAIETGIQNSGLGLVLIFNPNLFDGLGGMAFIAAFWGIWHIVSGLGIA
                     YYWSRKPL"
     gene            46905..48254
                     /locus_tag="PK049_02885"
     CDS             46905..48254
                     /locus_tag="PK049_02885"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOK60532.1"
                     /translation="MPTNRERIEEWSKAYWLLRFYQNFLFKLYFKTEIVGMEKLPPNT
                     TFIFAPNHQNALIDALAMLTLKHSWQPIFLARADIFKKPLVSKILTFLKIMPVYRIRD
                     GFENLSLNDSIFLKTMDIIRKGNGLVILPEGNHAGFKILRPLKKGIARIALQTEDASD
                     GSLNIHIVPVGLDYSNYVRYRSKLLIRIGEPFPVKKYLDLYRQNKALAYNALIDELSE
                     RIKAEMIHIEDEQNYYEYLFLTQLFPKRFIKENKLPNTLNQAFEISKRLILKLDRLKE
                     TDRSTFDEIIQLAHVLRDRISANRIKPQALPLKVSKAFNLLWMVPLFVVLSPLAIFGF
                     INHIIPIIIPYQLSKKFEDVQFHSSVRHAAGLLLLPLAYLIQTVIVGLILKSLAMALL
                     YLVLLPPAAVILYYWRKLFYKIRGLTSILIFAMRNRNEFELINNHFNRLMNDIKSIF"
     gene            48374..49126
                     /locus_tag="PK049_02890"
     CDS             48374..49126
                     /locus_tag="PK049_02890"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOK60533.1"
                     /translation="MLKALIVDDEIASLRTLELLLGHFEQHIEIIGVAYSASEGIDKV
                     TKYDPDIVFLDIEMPGGSGFDFLEGCPHRNFDVIFITAYNNYAIKAFKYSAIDFLLKP
                     IEVDELHEALEKVIRRRYSNFDGRNKYFALFENIKEIIPQKLVVSTNDKYECLDVGEI
                     VFVESVPNGTQIKLVNGSHFLANNPFGDICTLLEERNFSFINPSCLINLGMIRKISNK
                     PESNIELKNHELITVRGEYLERLNHQLENISK"
     gene            49136..49555
                     /gene="ruvX"
                     /locus_tag="PK049_02895"
     CDS             49136..49555
                     /gene="ruvX"
                     /locus_tag="PK049_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOK60534.1"
                     /translation="MGRIVALDIGRKRTGIAMTDPLRIIASPLETIPTHKVIDYLTEL
                     FLKENIDILVVGNPRQMNNTPSEAVNYIKPVINAIKKRFPEMPIELVDERFTSKMALQ
                     AMIDGGMKKKDRQEKSNVDKISAAIILQSYMEGLKYK"
     gene            49581..50132
                     /gene="def"
                     /locus_tag="PK049_02900"
     CDS             49581..50132
                     /gene="def"
                     /locus_tag="PK049_02900"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOK60535.1"
                     /translation="MIRTVYVYGSPVLRKVAQNITPDYPQLDALIADMFETMEQSDGI
                     GLAAPQIGLSIRLFVVDGTPLADDDPTLKDFRKAFINAQITERFGEKVIYNEGCLSLP
                     GLHEDVERESKVRIRYHDEKFVEHEEVYEGMAARIIQHEYDHLDGILFTDRVSPIRKQ
                     LLRSKLNAIAKGKYSASYKCRIG"
BASE COUNT        14501 a        10967 c        10277 g        14369 t
ORIGIN      
        1 cctactgcag cctttgagtt tagtatctga atggaaacct ttgatatggg atcagtttcg
       61 ctgtatgtgg ttttttcatt tgattttaaa cccttaaagg ctacgttgta tgcatctttc
      121 agcatcttga tatcgttttc gtttagctca atatcttttg aaagccctgt tttatcggcc
      181 aagaacttca gcaaatcgtc aaactttgcc tttgggtttt gttccttaaa ttcagcaata
      241 agcttatgta aacgctcctc ggatgcagtt tggtaatgga gtaactttac gtgaaaatcg
      301 tacttgtttt ccctgttccc aattgaaagt ccaccagttt cgtgatcagc agttaccact
      361 attaacgttt cgtgaggatg tttcctgtaa aattccaagg caacatctat agcacgggca
      421 aaagcaagca cttcatgtat cacagttgct gcatcgttgt catgggcagc ccaatcaatt
      481 ttgccccctt caaccataat aaagaaacca ttggggttat ccactacctc aatggcttta
      541 cgggtaatgt cggcaaggct tatatcggat tccgaacggt caatttcata ctgtaatgtg
      601 ctgctgggtg caggatttgg ggcagagtaa acaatggatt ttgctcctgc tgccaccaaa
      661 gagtctacct ccgttagcga tgatgtaaat tggcaacctc taacctttcc caactcgtat
      721 acgctaacgg cattggaatc ggttttggta ccatagggtt caaggaaacc acccgatgca
      781 aaaaaatcgt aatttgactt cagcagctgt ttagcaatat cgtagtaact gccacgtgag
      841 ccaacatggg cataaaaagc tgccggtgtt gcatggtcaa tgcttacgct ggttaaaatg
      901 gcaacttttt tcccagcttc cttaaacttt tttgcaatac tgtataaagt atctgtccta
      961 tcgtaagtta aaccaatggt gttgattgaa gttttggtac cggttgaaag agcagttccg
     1021 gcagctgctg aacaggtaac atacctgtta tgtgcatagg tagtagaaag cgaagtgacg
     1081 gggaaacggg taaacgacat tgccgtgctg tcgagtaaaa cgctatcggc atacagcttt
     1141 gcaagtgtca cgtgcgatac tcccattcca tcgccaataa agtaaaatac atactttgcc
     1201 ttgggttcat gtttcccgca ggaagccatg gctatagcga gcaaaattgt tattagggtt
     1261 cgtgctctca tctcttgtga tttaaaaaat ttattgaatg tattgataac aaaactacac
     1321 taaaaaagtt ggagcaacat acaagccttt agttcagttt attaaaaggt gttaaaaaac
     1381 aggaagctgc ccaagggcag cttcctgaaa taatggtata aaaagcaaat tactttttct
     1441 tatcggcctt gcgggcagcg taaacaatct tgagtgcact ggctttacgc aactcctgct
     1501 taacatcggt aacaataccc atacgggttt ccttgtcaat ttttagcgat gtggtaagca
     1561 agggcttttc atcttccttc attgcttcac gctccgatgc gataaaatcg cgaatatcgt
     1621 cgagcgactt gaaagaatcg ttaagctgaa tgcggggctc agtaccaaac agagattgga
     1681 actgcctcat aggaggacca atgtagatat acgataccaa cgacttcttt tccagcttgt
     1741 ttacttcagt ggcttcgggc agctggctac gaaccattag cgtggtttca cgcatggttg
     1801 tactaaccat gaagaagaag agaagcatga acacaatatc gggaagcgag gcagttgaaa
     1861 tagcctgcaa ctcgttttta ccttttcgtt caaacttagc cataactact ttcctcctac
     1921 gttcttaggt tcagcttcgg aaatcttcaa tgggatggcc ttagaaattg tttcttgctc
     1981 ttccttggct aacttgctga aaggctttcc gtaaactgaa gttgatagtt cgtcgcgtaa
     2041 ctcattgtat gcagcaacca gctcgttctg aaccttcatg tacatttcgt acgatgtacc
     2101 acggtcgttc tgaagagaaa caacaccctt tgaaacttta aacttaccta ttccaggtat
     2161 atccttttcc tcaaactcgg aaaggttggg gtcgctattc gggttcataa tgaactcctt
     2221 ggtcttctcc ttcaacatgc caacctccat gggttcacca cggacaagta acttatcgct
     2281 cttgttgata agtacagaga aaatattcct gcgcttaacc tcaacatcat cctgtttttg
     2341 attgggatcg ggcattggtg gaagcatcct cgtaagaccc gtatccacgt tcatagtagt
     2401 ggtcatcagg aagaagatga gcaataagaa cgcaatgtcg gcagttgaac tggcatttac
     2461 ttctggaact tttcttgcca tctttatact tagtattttt caggttagcc gaactgagcc
     2521 ggctaaccat ttgtttaaac tacttatgga atgcactgcg aacgccggta tataacaaca
     2581 atagcataga aatgccaaat aacaggtatg ttgctattaa accggtatcg ctaataatca
     2641 gaacggtagg atttgaaagg tcagcgtttt gagttggcga tggaattgga gtagcatcgg
     2701 ccaaaacata gccaattaca aagattacag caactgccag taatcctacc aacgatttag
     2761 tgacattctt ggggtttctt acaaggttta caacagggaa aactaatgcc gaaacggctg
     2821 ctatggcaaa cagaatataa gcccaatcaa tgaatggttc tacagattca atatcctggt
     2881 tgctcaaaaa gaatagtatg gttataacaa tagaaacacc cattaatagc cacgaaagaa
     2941 ccttggtata ggttgataat gagctattca tggccaatta cttttgaagg ttaaacttaa
     3001 caagaatatc gagcagactt actgaagcgt cttccatttc attggtgatg ctatcaatct
     3061 tattgagcag gtagttatag aacacctgaa gaataatagc aacaataaga ccgaacacgg
     3121 tagtgataag agcaacctta ataccaccag caacaagcga cggagaaatg tcaccagcag
     3181 cttggatagc atcaaaagca ctgatcatac cgataacggt acccataaac cctaacatgg
     3241 gagcaatagc gatgaaaagc gatatccatg ttaatccctt ttccagctga cccatcataa
     3301 ccgaaccgta ggtaacaatt gatttctcaa caacttcgat accctcgtga acacggtcaa
     3361 gaccttggta gaaaatgctg gcaacagggc cacgggtatt gcggcacact tcctttgctg
     3421 cctcaacacc accatttttt agagcatcct caatggcatt gagaagcttt ttggtattgg
     3481 tagtagaaag gtttaggtaa ataatacgct caattacaat agctaaacca aaaattaagg
     3541 ttaaaagaat ggaagccata aatccaggac caccctcaat gaatttggtt ttaatagcct
     3601 tgtgtaacga ttgttcttca acagcaggag ctgcaggtgt tgcagcctga gctgcttgct
     3661 cctgaaccat tgctgagtca ggttttgctg caggttcatc ctgtgcatta acaaaggttg
     3721 atgctccaaa cgagagcact cccaatactg ctactagtgt gaatagcttt ttcatggttt
     3781 gattgtcgtt taattattac taaaggtttg ttttgtttgt tctcaaattt agttaaacat
     3841 taaaaggctg gcggagagag agggattcga accctcgata cgcttttggc gtatacacgc
     3901 tctccaggcg tgcgccttag accactcggc catctctcca aagttacgaa atactaaata
     3961 aagaaccttt aaaacgtggc gcaaattaca aaaaaaatat tgaaaaaaac aatgactatt
     4021 tcaatgttat ttcgcctgct atggattttt ctaactcatg ggcaagcgcc ttaccataaa
     4081 accctttgcc aagcaaaaac attagcttag caactgctgc ctcataggtt atatcgtggc
     4141 cacttactac acccaaccgt tttagcataa ggccattctc atacttatcc atgtcaaccc
     4201 cgccagcctt gcattgggta acgtttaaca caactatatt actgtttata gcatattcca
     4261 gtttctttaa aaaccacttc tgcattggtg cgttgcccga gccgtaggtt tccagtataa
     4321 ccgcttttac ccctttggtg gttaaaatgg attccacaac attttccgat attcccggaa
     4381 aaagtttaag cacagcaata gagctatcga gctgtgtgtg gacaataagg tgctgttcct
     4441 gagtagggta atgaataaaa gggtaattgt actttattga tatacctgcc tcggcaaggg
     4501 ggggatagtt gtcggaacgg aaagcattaa aatgttcgga gttatgcttg gttgttctgt
     4561 ttccacggta tagcctattc tcaaaaaaaa tggttacctc cggtaccatt gcctgcccgt
     4621 tttgaaggga ggcggcaatc tcaatggcac tgataaggtt ctccttccca tcggttcgca
     4681 acatgcctat gggtagctga gaaccggtaa gaacaacggg ttttgacagc ccctcgagca
     4741 taaagctcag agcagaagct gtaaacgaca tggtgtcggt tccatggaga atcacaaaac
     4801 catcgtagct ttggtaatta tccccaatta ttgccgctaa cttcctccag aaatcgggat
     4861 tgatatccga tgaatcgatt gggggttcaa aggagtatgt gctaaggttg aagccaaatt
     4921 ttttgagttc aggaacctcc tgttcaattt gctcaaaatt aaagggagcc aatgccccgg
     4981 tttcagggtt ttgcttcatc ccaattgttc caccagtgta aataattaga attgatgtat
     5041 tacccgattt catctatctt aattttgaag agtttaacgg cattttgggt ggtttgtttc
     5101 gcaacctgct ccacagtaac tccgagtaac tcggctacct tgtcagcaat gtagataagg
     5161 ttatagctct cgtttcgctt gcccctgaca ggcactggcg ataggtaagg ggaatcagtt
     5221 tccaataaaa ttttgtttgg atccaactgg tttactaaac cgttaacccc accattcttg
     5281 taggttacaa taccaccaat ccccaccaaa aaggttttgt attcgtcaat gtgtttataa
     5341 tcgtcaatag ttccagtaaa gctgtgaaaa actcccttta gggtgttgtc aatttcctga
     5401 tcgacaatat caaagatctc cctgaaagaa ttgcgggaat gaattacaac cggaaggttg
     5461 agctttttgg ccagcctgag ctgatgcctg aatgcctcct gttgctgtat aaagtatgtt
     5521 ttatcccagt acaaatcaat tcctatttcc ccaatgcccc agaacctggt cttggccaac
     5581 tgctcctcaa cgtgatagat ttccctttca aacccgttcc ggacagaggt aggatgcaaa
     5641 ccgattaaag ggaagcattg cggaaagcga ttgcaggtat cgagcaaacg gttggtagag
     5701 gaggaatcga tatttggcaa aatgtgataa tgcacaccaa tctcggcagc acgcataaga
     5761 acagcttcac gatcggtatc gaactcgtcc gagaatatat gtgagtgagt atcaatcaaa
     5821 agcatggttt actctttttg aactgcatag tagattctgc ttccgggtgt tatgctatcg
     5881 agttgtgctg agagtttggc tactacattc ctgaaagttt caagattttc ctgtttaacg
     5941 ggctccaccg gaggtgattt catttttaga gggtcaatgg gtgagccatt catccaaaca
     6001 cgcatgtcga ggtgtgggcc agtggcatag cctgtacgac caacataccc tataatatcg
     6061 ccctgtttta cacgagcacc ccttgcaatt cccttggcaa atcgtgaaaa atgattgtag
     6121 ccagttgtat atacgctgtt atggcgaatt ttaatgtaat tgcctgcggc cttatcgtaa
     6181 cccatatcaa ttacaacacc atcgcctagg gccattacag gtgttccttc aggagcagca
     6241 taatccacac cggaatgagg gcgataaatt tttagcacag ggtggaagcg gttcccgcta
     6301 aaacggcttg aaatacgtga aaagcggagc ggagctttga ggaacgactt acgaagtgaa
     6361 ttcccttttt cgtcccagta gctggctatg gagtcctgtt caaacctgaa cgcgtaaaat
     6421 ttttcgccac gatgctcgaa ccatgcagca taaacagtcc caatcccaat tgatgtgcta
     6481 tcaacataaa gttcatcgta catcaccctg aacttatcac ccttaaaaag gccaaagaaa
     6541 tcgactgtcc aagcaaaaat atcggaaagc tcaagggcaa gcatgggatt tagaccattt
     6601 gccttcatat cgacccaaag cgacgatttg atttcagcct cggaaatttt cctaacaggt
     6661 acaatctctt ttttaaaggg gtaaacgtaa agggaatcgg taaggttaaa aacgaaatat
     6721 tcaacaggcg aaacctcata aacaagataa gcaggagtct gaagggaatc cttccgggcg
     6781 aaaaccttat accgtgcccc cgccctaacc tttttcacat caaaaatatc gggcgtaagc
     6841 gagagcagaa actcattaac atttccagtt aggtttaagc tgaaaagtat tttccctagt
     6901 gtttggttac tcctaacggt gtattcagta acataaaacg aatcgattgg tattccatac
     6961 tccagtatgg gttgcacatc ctccaccaat ggtttttgat ccttttcagg gctgtatagt
     7021 ttaatgtagg cggtacctat gaaatagtca attatgaaca ctgtgagtaa agaaataact
     7081 aaaaggggta aaatcttttt aagcataaat tttctgaatt ttgccccaaa actaccaaaa
     7141 aagaatgtaa ctgcattttg gttattctat tttgatgtag gtttgcagaa aaattaaaaa
     7201 gatgagcgaa acgcagaaaa gaacagagct ttacgaactg ggtgagttta agctgattga
     7261 taggttaact gagccgtttg ccccaaaaca tcaagagaca ctaaaaggtg tgggcgatga
     7321 tgctgcggta atatcatgcg gaaaaggtga atgccttgtg gttactaccg atttgctact
     7381 tgaaggaatt catttcgact taacctacac accccttaaa cacttaggct acaaagctat
     7441 tgtggttaac ctcagcgatg tttacgcaat gaacgcaatc cctaaacagg taacggtttc
     7501 cataggcgtt tcgaagcgtt tctgtgtgga ggatcttgat gagctataca gcggaattaa
     7561 gcttgcctgc gatagctaca atgttgacct ggtaggtggt gatacatcgt cgtcgatgac
     7621 tggaatgact ataagtgtta ctgctattgg cagcgcaaag gaggatgaaa ttgtttaccg
     7681 gagtggtgcc aaggtaaatg acttaatttg cgtaaccggt aatttaggag gggcttacat
     7741 ggggcttcaa ctgctggaac gcgaaaagag aatcttcctt tcaaataagg cagccaagcc
     7801 aaagctggaa ggttacgact acattcttga gcgattcctg aaacccgaag tccgaaggaa
     7861 tgtaattgag aacctacgaa aggcaggtgt taaacctaca tctatgatag atgtttccga
     7921 tgggctatcg tcggagctgc tgcatatttg caagaactcc aacgtgggat gcaggatata
     7981 catggacaaa attcctattg atgagcaaac cgcacgaatg gccgaggaat ttaatattga
     8041 tatgtttact gctgcactga acggaggcga ggactacgag cttttgttca cggttccgct
     8101 atcggaatac caggtagttg atagcatgga aaatgtcaga gtgatagggc atattgttga
     8161 gccacacctt ggtgcataca tgataccacc atcgggtgaa gacataagga ttactgctca
     8221 gggctggaat gctttacaaa aggaatagca ataaaaaacc ccgcttgagc ggggtttact
     8281 tttttttact cgttgataat tcctttgtac tgctcggcat tgagcaaatc gttcatctca
     8341 gcaggattgg acattttgat tttaatcatc caaccttcac catagggatc cttgttaacg
     8401 atatcgggtt gttcagtaac cttagggtta acttcaacta cctcgccact aacaggcata
     8461 aacatatcgg aaacggtttt aacagcctca atggtgccaa aaacctcctc cctggcaagg
     8521 gtttcaccct gagtttcaat ctcaatgaaa acaatgtcgc caagctgtgt ttgggcaaaa
     8581 tcggttacgc caacggtagc catgccattg ccttcgtcct tgatccactc gtgatccttt
     8641 gtgtacttta ggtttgatgg aatattcata gtattttcag ttaatagttt tacactaaag
     8701 attttgctgt tcaaaagtag tgaattttaa gattgtatgt tcaatcctat cggttttttt
     8761 aattagtgcc gcatgttatt cagcacatta gttaaccaat tcaaacctga cgctaaatcc
     8821 aaagctggta ttggttgttc ggaatgttct tgaaacctta gggttattga gggtgcggtc
     8881 aaagaacagg cgaatggtta ccttctcatt aatccggtag tcagcagctg ttttgatagt
     8941 gctaatcagt tgacctgcaa taatatcgtt cgaatcctca accaactttc gaagaatggt
     9001 agtattatcg cgtagcgaaa aatccacagt taatcgtaaa tcgctcttga ttgccttttt
     9061 accaccatcc tcggcctgga aaataagagg taaatcctca aaccggtaac cagctcctac
     9121 gataaactcg ttgccataaa tctcggtaag ctggttattg gcaaagctca tggctaatga
     9181 acggctattc ttaatctcga tcctgttggt aaggttattg ttccatccca aatcgagccc
     9241 aaccaatggg gtaaattgct cgtttatagc aacgttagtg atctcgttct gcgggacaaa
     9301 atcgccgtta atgttacgaa cgtagctaaa cccatcatcc tcgggtaaat aggtaagatt
     9361 gcttgtaaaa gccccaatgg tatagagcga tcgataccca tgattgatag taattgactt
     9421 aaaccaacgc ttaaaaggtt tcatcttggc caaaccatcg tacgatagct gccagttagg
     9481 cattggtata aggggaaaat cgcttagcga gaccttgctt ggggatgtat tggtgtaagc
     9541 ggccaaaaac gaggccctga gaacatcctg cgataaaggc ccatagccat cgggaaatcc
     9601 tgtaacagga tctatcacat tggggtcata gccctgcgat ggatttgcaa ccctcctatc
     9661 gcgaagccta taggcaattt ccaatcggtt ttgcttcatc ctttcaaaag ccttggagct
     9721 tccagtccgt ttaccaccct cgaatgcagt acctatggta agcacgctaa tactgaaatt
     9781 tcctgttgtg agcggactta gcaccctgta agaacccaaa ccatcggatt taaaaatttt
     9841 gctattgctt ttacccagtg ttcgcgtagc gctaacatcg aaacggaagc ccggtaaagg
     9901 ttccagtgtt gctctgaagt taagattctc attgcgagta aaaactactg gtgtgttaaa
     9961 cgatgtgtcg cgggtgagcc aaccattagt agcagccttc caagcaaagt cggggtcctg
    10021 ccaaccagct acaaattccc agccgggggc aagcataccg ttaaagttct cagttcccaa
    10081 gtagtttacc ccaggtttat aaccaggtag tagtgttccg tttgtttccg tgtacgatgc
    10141 tcccatgctt tttacgctca tcaaaagtcg gagtgtggtt tcaagggcaa acttggcggg
    10201 gttaatcttt tcagggactt tgccttcaac cacaaccttg cagttcttat aatcggcatc
    10261 ggccttaatg gctaccttgg tgtcgccacg tacctgtgaa cttaccttta tctccttgcc
    10321 attggggtcg aaaaccttta cggtaacctt ttccgttttt agcttgtggg taataacctt
    10381 agttgcattt gccctaaggt ttaccttttc ctcctcatac ttaaccgttc gggtttgggg
    10441 tgcagccccc cttgcccgct ggtcaaactg ctggttaacc cttttaaggt atcctacctt
    10501 attaaataag gtattaatgt ttacctgccc attcagctga atggtgtttg agttctgaac
    10561 tgtattcccg ggatcgaacc gtgaagtgtc ggccaaaatt ggcccggcct gccaacggta
    10621 tattccggtg taacgcgatg taatgctaat ccagttgagt aatggaattt tgcttaacgg
    10681 aagggaatag gttatgttaa actgatggtt gtacatggtg tttcgtccac ccctacggat
    10741 attctgccaa accgagtcct tccactgctc atacctgtcc ctatccctat acctgtccac
    10801 catgccctca ggttcatcga tacgagcagt agctgtagcc acaaaatcaa acttaaccga
    10861 ttgggtaata tcccagttca gggagtagtt cctattccac ataaaatcct ttgaaaatgt
    10921 gggtaaaaat acctgattgg ggttctgaat atttcggaat tgctgttcaa agtaattcct
    10981 attcagatcg gtacggaacg agagctgggt tgggaatagg ttaaagttaa aatcgcgtat
    11041 taaagccaaa tagcttgaat ttaaccattt tatcttgcta aatggggata tatacttggg
    11101 ctgtcgatta taattatagg taaatgcacc ccgaatattg cgctgaatcc tatgatcaat
    11161 tctaacgtta cgggagaaaa tctcgctgta agagtagctt accgaaatat tggcgggatc
    11221 ccataggcgg ggcttagagc ccattttatc aatctttaca ttggtgaagt tgatacttcg
    11281 cctgcgagta taatcctggg caatccgttt aatggaatct ctttcgcgtt ccgatgcgtt
    11341 acgtagggca tcgtcgagaa gtatatcggg gtcaagcggg ttgtactggg ggtttgccat
    11401 cgattcgccg aatccaatga acattggaat tttaatgttg aacgaggcgg ggaaaaactt
    11461 acccagctca agggttgagt tgatatcgta ctggtaaact gtctccttgc tccgctcgtt
    11521 aacctttttc tcaatggaac caaaaccggg tgtaatggtg gtgccagcta cggtaaccat
    11581 tcccaaatcg gcaagttttg ccgataagcg ggcattggct gcccatccac tttgatcgtt
    11641 aaagttagta agccttaact cgttgaacca tgctataacc gatttaggca ttctatcatc
    11701 ggcagggttc ctaattccta tcattattac cttaacgttg ctaaggttcg gatttccggt
    11761 aacttttagc ttactgttcc cgctttgaac aacatacaca gtattcaggg tgataagcga
    11821 ccctggtttt tgcatggcat tgtttcgggc tactttaaca tcaaccagct tatcgaggtc
    11881 gatatcaatt acattctccc ttggccatac cgcctcgcga tcgcgttccc ggctattgta
    11941 gtaaaacccg taaggagtca gctttaatgg tatttcgtac tcgtagtagt tagtgttata
    12001 gtcggtacct atacggatga aagcatttac ctcgtagtcg tttaatggat atccctcaat
    12061 tgcctcggca tgaacatcca ttttgagtcg tttgtactgc ctgatatcga tgttaacatt
    12121 cttaaacgct gcacgggcat cgccatcggc caaatcggtt accctgtact ccatggcctg
    12181 ttcgttaagc tggataagct ggggttgact tgggtcaata accctatcca cgcctggtgg
    12241 caatacgtag ttaacgggtg ttcgtttgtc gttctcctca atatttacgg ttgctatcga
    12301 taagctgcct gaaggtaaat cgccaactgg cataccctcc tggccttgaa taagtgagta
    12361 attgtattta cgccactcgc tacgaaccag atgcagcgta gcaaaacgta gtataaccgt
    12421 atcctggaaa ccgcgcagga acatcctcat gaaacggatt gacttgaaat cctcaattgg
    12481 gccaaccacc ttctcgtatt ctgcaatggg aaccttaaat tgataccact ttactgcttt
    12541 atttaaaatt ttatccttat cgcctgtcac aacgttagtt ataaaattct taccaacctc
    12601 catgtcctgc gggcgaagtg aaatgcgata ctgatagtaa gcctcatttt cattcatggt
    12661 attatcggtt cccagctcct ccacatcggg ggttgagtag ctctgctgcg aggtagttcc
    12721 agttgcaact ggcgaattat tctcggttcc gttaaagtac ttatatcggt cgagaatggt
    12781 ggcacgctgt tggtcgtaaa ccccgctaag gtaatgcctg aagttatcgc ctgccggatc
    12841 gctttcaaat ccggcgagca catcgctatt ggttatttta ttcctgagct ggtttatata
    12901 ctccgaaaag aagaactgct catcgagtat cccatcgcca tccaaatcga ccgacgagga
    12961 gagtccatct aatccaatat cctggtaggg gcgcttagct tggtcgttat cgaaaccaac
    13021 aggcagatac tgcttagtag gaaccctacc ccaggcggtt tgatcggttt tggaaagctc
    13081 ctccgatgag gtgggtagtc cattctcaaa cgattttcgg ccatccctta aaatatcctc
    13141 ggagatgtta ccaaggttga tgtaaaaatc gccgccacta ctgagcgtgt cgtacacaaa
    13201 cggatccatc aaccaaaact caatatactc aatatttgat gcttcaaagt cagtggtgct
    13261 caggttgcgc attataccac cccaacggcg ctgcggatct attaaccttc cgtttgcatc
    13321 aagttcagta gtattaaagt tatactggcc acgttcagta ggatagaatg caaggttaag
    13381 aacggagata ttggttggtt gcccctgtgg cgtgttcttg tttggaaaaa gctcgcgctc
    13441 aaatatttcg cgaacaaaat ggttaccctg cagctcctta ttattacgga gatacgatgg
    13501 cgtaagcgac gtgttcctca aaaatagcgg atcgatgtag taccaagcca aatgggcgcg
    13561 gttgtatcca taccgtaaat cgttggagaa cgaggcttcc gggaacatat cgggttgccc
    13621 ctggggagta cttgctaact tccattctac ccatgagcga atatcaagcg atgtgctgct
    13681 cccctcaaaa tcatcgatat atactgtacc ggccttacca atggcacgcg agtgcccagg
    13741 aattagctgt gcaaactcag cgtcaaatgt tattgttgat ttttccttgg tttgaatgaa
    13801 tggaagaaag tcgaccattt tagtcaggaa cttggactcg gtttggtacg aggtattaaa
    13861 tccccatatt gtattcgata ctgcctcgtt tccaaaactt accttttggg ttagggggcg
    13921 ctccgtgagg ttcattatgg ttgccccaac attaaagtta tcggagaaac ggtaatcaaa
    13981 gtgggttcca accaaggttt tggtttgtag gctgaacaag gcgttgcttt ccactgcaac
    14041 ctttatggga gtaccggatt caagaagtcc ctggttgata attcgtacag tacccagatt
    14101 gtaatcaaca ataaaatcaa catcctcctg cagttttaca ccacctgcag ttacaaccac
    14161 ggcaccttta ggaatattgg gcgcattaag aaagatttcc gatcctccgg ccgacttgta
    14221 ggaaccccct attaggaact tgttcttatc ggctacctgt cgtgctttgg ttaacgatga
    14281 atcgtaaagc tcttggaaaa tatacttctg ggcaatagta ggatcgccta tacgcgatgc
    14341 caaatcgcgt ccaaatggtt ccaaaacagg aaaaataatt ttgccgttag cgggattaat
    14401 ggttacaccc tcaataaagt caaacactcc atcggggaat gcatcgagct ggttgttcag
    14461 gttatcaaga ttaagtactc tgattagcgg aacttcggca atattccctt ccttgatata
    14521 gttaatcggg gttccggttg catcgtcctg gtaaaaaata tcgagcctga aatcatcttt
    14581 gctaatctga taggcattca tggagtagat gtttttcatc atcaacctcc atgtgggcat
    14641 tttagggcta aggtttgttc cctttaggag tttaaggatt aaggctttag gagcctctac
    14701 cccatcggta gagaattcac caaccttgta ggtttggcca ttgtatgtgt actcatatgc
    14761 aaccgcaagt acctcatcgc tattgagcgc cgacgatagc gagatatacc ccaacgcctg
    14821 atttatggtg aactcattgg gtgatagctt acgggcatac tcaatcttct cataatcgcg
    14881 cccaccggta aaaccctgcg attccaaggg caacaataca ttgcttactt cggcaacatc
    14941 ccgaataccg gcgtaggttg tggtcataag ctcgtacagg ttgtttaaac cgttgcttgc
    15001 cgggccactt cccgtttggg caaaaatatt ggaagcaaag atgttgttct gggcctcgcc
    15061 caaatcggca aaagcaatta tattgcggga gttctcaaag tttgcctgtt tgtttgttac
    15121 ccacacctca attttggtga tttgtacccc tgagttaata atgggtaaat tcatcagggc
    15181 acgattgtag ttttccctaa agtagtgaga aaggaaaaaa tgcctattgg catcgtactc
    15241 atcggcttta agctgaaaat cgcgcgtttg cgcaccaccc ttaatctcaa ccacctgtgt
    15301 ttggccattc tgtttggata ctacgctagt aacgtttaac tttccgaact taagctgagt
    15361 tttaatacca aaaaggctct gactcccggt tattagagtt ccgggtaaag gcaacgaaac
    15421 gttaccagcc tcaatgcgct gaatgatttc atcctcaaat ccgttgtact ctattttaac
    15481 attattctca aagtcaaagg tcgcctcggt gttgtaattc acctccattt ttagcttctc
    15541 accaatctta ccggaaacgt tcatctggat tttcgactga aagtcgaagg ttgtgttccg
    15601 tcgctgatcg attggtaatg ctgggttatc ggttttagcg cttgatactc caaacaccag
    15661 ctcggcatta ccctgtggta taatttctat ggtgttgctc ccaaaaattc ggtcgaatgt
    15721 ttcaccacct acctgaatac ggggcagaat gccatcgcct ttccccacaa attcaccggt
    15781 ttgcttttgt cgccagtact cgcgcatggc cttctccaca cgatacgacc ggtactcatc
    15841 ggccgatagc acacgtggtg tttcaatggt tgtatttcca acctttctgt aaatgatgta
    15901 ctggttgttg attggatcaa acacaacctc ctcgcttatg tttgatgggc ttgaaaggta
    15961 taatggtgaa tactgcgtgt caattgacca tataccgggt tgtttaatag ggaaaggcaa
    16021 atttaatgta tcctgttgtg gaatgggaac agaaaaagct ctcgtaatgg taatggttac
    16081 gagagtaagt attccaatta ttcggatagt atgtgtatga gataagtgtt ttaccctcaa
    16141 cttacaaagc attttactgt tactacaagc gtttgagcgc atgcttaatg agcgcttcaa
    16201 ctggcatttg ccccatttcc ttcagaacct ggtcaattac cttatcggca gctgaacggg
    16261 caaagccgag catcaccaat gcagataacg cttcgctctt taaagtattg tttgatgttg
    16321 ataaaatttg tgaaaccgta gcctccccta tggaaacctt atccttaaga tcaacaacta
    16381 tgcgttgtgc cgttttaagg ccaattccct taacctgttt tatgaggtta acattttccg
    16441 tggtaatggc tgcctgaatc tcatcggtag taagcgacga aagaataagc cttgccgtac
    16501 tagcaccaac tccagaaact gaaataagca agcggaaaat ctccctttcg cgtacatcgg
    16561 tgaaaccgta gagcaaatga gcatcctcgc ggataatttg atgaagccag agcttaacct
    16621 gtgttttacc ctccagctga gagtatgttt gtaacgaaat atttacaaaa tacccaactc
    16681 caccagcatc aacaattgca tgggttggtg tcagctcggc aagggaaccc tgaatgtact
    16741 cgtacataaa tcaacttttt tatgccgttt ctgggtcaac tgactgcaac gggtttgagg
    16801 taagctcctt atgcagctgt cggttacgga aaatatcaat ggcaaggtaa accgcctggc
    16861 ggaatgagtt cgggttagcc aggttatgtc cggccaaatc gtatgccgtt ccatgatcgg
    16921 gcgaggtgcg aacaatgggt aaaccagcag tatagtttac tcctgtatcg aagtttatca
    16981 ccttaaaagg cgataagcct tgatcgtggt acatggccag aattgcatcg aaacgggtga
    17041 acctatcgga gccaaagaaa ccatcggccg gatagggtcc catggctact attccctcct
    17101 cgttggcctg ctttattgct ggtaatataa tcgtttcctc ctcgttgcca ataacaccgc
    17161 tatcgccagc atgggggttc aagccaagta cagcaatacg aggccggcgg attgcaaaat
    17221 ccttaattag tgtctcgtta agcaagcgta acttgttgag tatggcatca acagtaatgt
    17281 tttttggaac atcagcaatt gggatatgac ccgtaacaac acctaccttc atggtatcgc
    17341 ttaccatgag cattagcgcg cttttaacac caaactcatt ggccaagaac tcggtatgcc
    17401 ccggaaagct aaagctttga ctttgtatgt tagccttatt gatgggtgcg gtaaccagta
    17461 cgtcaatttt accagctttt aagtctgcta tagcagcctg taatgctagc aacgcaccct
    17521 gtccgccata ctcagttgat ttacccaact ccacccgaac gttttcgtcc atgcagttta
    17581 caatattggg gcgtttgggg tttgcctctg aggcatcctt tatttggtta aagctgaagt
    17641 tttcaatgtt caacgctttg cgatgatagg cagcaacctt gggtgatccg taaacaattg
    17701 atgtgcacat ctcgtccata cgagggtcca tcaatgtttt aataattacc tcatagccta
    17761 ttccgttaat atcaccctgg gtgattccta ccctaatctt ttcattactc ataccttgta
    17821 gtattttaaa tggctcgtaa atatgcaaaa gtaaaaaaaa gctgtaaaca tagttaacac
    17881 cttgttgttt ttactaggaa tgtttttgtt acttttaata cctttgccca atcaacattg
    17941 aagaatgcaa gttaaagata ttttaaaaaa ggcaataaac cttgaatccc tcaccttgga
    18001 agagggcctt tatatttaca cacaggcatc aacgcccgat ttgatgtatg ttgcaaacga
    18061 gcttcgcaaa attcatgttc caggccacaa ggttacctgg cagattgacc gcaacgtaaa
    18121 catcacaaat gtatgtatat cgggatgcaa gttttgcaac ttccactgtg ggcttcagtc
    18181 cgatagcgcc tttatcacgt caattgcaga gtataaagta aagattgaag agcttgagcg
    18241 ctacggaggc gaccagcttc tactacaggg tggacttcat cccaggcttg gtgttgagtt
    18301 ttattgcagc ctattcaagg aactaaagtc aatgtttccc aacatcaaac ttcacgccct
    18361 agggcctcct gaaattgcct ttatagctaa aaaagaaggt atgacctacc ggcaagttct
    18421 tgaagagctt gtggaggcag gcctcgatag tttacccggt gctggtgccg aaattcttgt
    18481 ggacagagta cgaaagcaaa tatcgccggg caaacccgat agccaggcct ggctcgatgt
    18541 tatggctcaa gcgcaccaaa tgggtcttgt tacctctgct actatgatga ttgggcatgt
    18601 tgaaaccctg gaggagcgtg tggagcattt gattaaactc cgtaacctcc aagcgcaaaa
    18661 gcctgccgac gcccccggat ttctgaactt tattgtttgg acctttcagg atgagggcac
    18721 caaactacag cgcgagggcg tgctaaatac ggtaaccact gaggaatatg ttaggactat
    18781 agccctatgc aggatcatgt tgaataacat tccgaacata caagcatcct ggcttactgt
    18841 tggtatcgat accgctcagc tgtgcttgca cgctggggct aacgattttg gctcaataat
    18901 gattgaggag aatgttgtat catcggctgg agccaagtac aagatggatg cctatggtat
    18961 tcagaaagcc attgttgatg ccgggtttga acctcaactc cgcaaccaac gttacgaata
    19021 catagtactc tccgactgtg agtttagccg catttacagc attgaggagc taaaacaaaa
    19081 caccagccca ctgctctaag aacaaataag ccggcataaa gccggccaac caaccaaaaa
    19141 cagttatgac gggagtacta attacttttt cttgagtagt tctttctcaa taatctcctt
    19201 gaacattttt ttagtttcct caggggtttg agctatacct gaggtagcaa aaggctttcc
    19261 gtccatggga aagaatataa atgttggtat gctctgtata ccaagttcag cagagatctg
    19321 ctgctcgttt tgggtgttca ccttgtaaac ataaattttg ccatcgtact ccttggccag
    19381 ttcctccagg attggacccg caatacggca agggccgcac cagcttgccc agaaatcgac
    19441 aatggcaggc ttatcgccaa ggtatttcat attttcctgt cccttggtgt aatccaaaat
    19501 tttggtcagg aaaaagtcac gggtaatatc aatggcatat ttctctttag ctggttttaa
    19561 tgcagtaacg ggttcacctt cgttttgctt ggcggcggtt ccgttacatg ctcctagcgc
    19621 aaaggctgca accaaaagaa ccgggaaaaa agcagtaatt tttctcatag ctatctctat
    19681 tttaatattt tcataatcaa agggcagggg ttataccctc cctttactac ctgccattat
    19741 cggcaaaggt aaatgcaccg tacccacaaa tcctaacgca caaaccgcaa ttcttgcagt
    19801 tttccttatc aatctctggt gcgctattcc agctacgctg tttaattgca cctgttgggc
    19861 agtaacgaac caccggacaa ggatggttct gcgggcatcg tgcttcatca acaactatag
    19921 tgctcatttt gttcattttt ttcggctaca aaaataatat attttcatat atcaatatgt
    19981 taatttatgt tgatatttaa caatgagcac ggactgtcaa attatgagca aaaggagatt
    20041 ttaactgaac cccaaaacta atttaaaatt cacataaagg caataaaaat aaaaaaggag
    20101 ggtttcccct cctttttatc tttctagttc ttattttact acttgccacc cctcagcttt
    20161 acctcgtagg ttttgccatt tactgttacg taggctatgt tatcgcaatc accattaccg
    20221 taatcaatag tggcctgctt atcgcctaca gtcaaatcaa tcacaccttg aaccaaccac
    20281 ctgcacgata gtttgaacat gagtgcactg gtaatttggt gagtgtaagt gtaacctcta
    20341 cggtttacgc ctgtggctac accttccagg gtgtaaacat catcccaaat gtagaacgga
    20401 gtatcataac cttcaaccca agtacgggtg cgctctacag tacgggtgta ggtggtacca
    20461 tcggtaaagg taattttacc attagtaagt gtaatctgat actcatgctc agcagttttg
    20521 cggatttgtt ttgttccttc aatcaggtta ccgttaatgg taaaattttt gaaggtaaca
    20581 gtacgctcaa attcagcacc attatactga ccacaggtac ggattacaat taacccgttc
    20641 tttactctct cgtaacggga gttagcattc acaaagttct cataagttag agagtcgatg
    20701 cggcaatttc cgtcccagga tgttttacga gtacgtgtac cttgaccacc gagtgttgca
    20761 tacatcagct gcgagtcctt cgaatcacca gcaagtgtaa tttcatccac ttcggtcatt
    20821 acatcatcgt agtaggtatc cacatcggcg tcttcgctag ccagggtaac cactgaatct
    20881 acttcctcgt ccttttggca tgaggagagt gtaaaagctg ccattaacat aaggcctgct
    20941 gcaaaaatta aacttgtacg tttcatagct gtaaaattta agttagacaa ttggttttta
    21001 aaatcattta tttgctcgta attactatta tttcaccact gtaatcccac tttaaatcaa
    21061 gcggtatgca caccaggttt aaagcttcgt ccaggtttgt acgtttgaag tagccggtat
    21121 aaaacctact tgtgtcagta ttaaggaaaa tatccacatt gaattgacgt tctaattcct
    21181 caatcacagc acttaaagga gcattctgaa aaaagaactc gttgttagtc catgcgggtt
    21241 tagactccgc ttttgttgtt tgtgttttat ttacatctaa agcatatccc attgtttttg
    21301 cctccatacc tttagttagc aatgtttgcg acttactgtt tgtggcaact acttttactt
    21361 tgccttcaaa acaggttacc ttaactagtc cattgcggct aaacacattg aatacagtac
    21421 caagaactgt tgtggttgca ccagttgtta ccactttaaa acgacgcccc ttgctaacct
    21481 caaacaacgc ctcgccagtt aacgatacac tcctatcgaa aaaccatcga ttcttgttat
    21541 aggtaatttt cgattcggca ttcagagtaa ccctgctact atcgggcagg taaaccacac
    21601 cgtgttcacc acgcgctagc tgcacgtgca catttcccat cctatacaaa tagatgtatg
    21661 aggtaagcat tagggcaact aaaactgatg ctgctactgc aacacgagtt aatgtcaatt
    21721 gtattactct tccctttgag tcttcctcaa agtttttatg gtcaattttt tcaaagatcg
    21781 cttcccaggc ttcggaacgc attttccctg caggcacatt gtagctatca agtagccttt
    21841 caatgttttc aggtagcctt tctattttcc cgtcccgagt cattttgtta tccttttgct
    21901 attaatacaa ccctattcca aaacacccta ccgtatccac aaaaaaaata attgaacttg
    21961 ttctgtattt gttttgctac taatacatct accttaaaaa ataccctacc gtattacttg
    22021 aaaatttgat aatgcccctt ttgctttagc caatcatttt aatggggctt tagtaaacca
    22081 aacgaagaat aaatttttag aagatttttg tttgatttaa tttgattttt taaatttgtg
    22141 catctgtttt tttacccgat atggaaaata aagtattgtt taccgattcc gttgttgcaa
    22201 acttttgcaa agctaccgtt gatgataacc caattcatga tccagtgttc atgtacaacc
    22261 agaacaaggg agttgttgtt cccggcatgc ttttattttc aacaattgtc aatttgcttc
    22321 acaggaaaac cgacgagtat tttaattact acaaactaat ttttggcaac gtaatttgta
    22381 ccaacgagcc tattgactta ggttttgaaa ccaaaggcga tcaaaattac ctatatgcca
    22441 taaacgggca cgatatgttt tccacaaaga acgagaggtc agtagttctg aaaaatatcg
    22501 agcccaaggt actcgattta gatggcattg taagagctat gcctttccat aaaacgcagc
    22561 tggaaacctt tagggagcta accaacagct tgaacaacac cctttccgat ttccttttta
    22621 caatagcata cgctagcgct gctcttttta aagccatccg agagccaata accgaagtgg
    22681 aaacggaaat taaccgtttg ctaaataaag ccatcaatcc tgaacgggtg tcaccctttt
    22741 accaaacact tgaaatattt agggtaaatc cgcatgctga gctaacgcca gaaggaacta
    22801 ttgaatacaa tattaggttc aacagagaaa aagccaataa aatctatgta gcccaagtga
    22861 aatgtatgca taacagtcag tgtttttacc aatcggtgta ccggttggtg gcaatacccg
    22921 ataagctgat aatgagaatg gtgaaggata gggataaagt ttaaaggtta aaggataaag
    22981 gttaaaggat aaaagataaa ggttgtctag tcctgaaata aatctacagg ttagaaatag
    23041 ttttaaactt aacttattaa aacttgatat taaaaaagaa tgtgggggta atgctgtagc
    23101 cgtaaatatc gtgatactca atgattgatt tgccctgtag gtaatcggaa ataggtgtat
    23161 cggtaagcgt gtaggggcgc gcaagcatat tatccctatt ataaaggtta aacactttta
    23221 caccaacctc aagttcacca ctaactgaaa cctttgccaa gtagcttaac cctgcatcta
    23281 acctgtggta cggctctaac ctgctcgagt ttttttcgta atcaggtgaa agggtaaggc
    23341 ttggagttag caagagctca tcccagggtt taggagtacc aaaaatccaa gttccaacaa
    23401 ctctccattt tcgccacttg tataaaccaa ctaactttaa ctcatgcaaa tggtcgtcga
    23461 gggcatagta ctcttcgccc ccgttcaggt ttgatgactg attgaaagcc ttactcagcg
    23521 aatacgatac ccaaccctcg gcattctgcc atgtttttcg gactaaaaca tcaaccccca
    23581 atgaacgact atcaacataa aaaattttgg attgctctac aaatcggttg gttgaggttc
    23641 gccggatcac agtttgaacg tacgataagc cctcggtttg ctttgcatat ccttcaatat
    23701 ctaatgttaa tccattgctt gtgtagttca aaccggtaac aaagtgcttt gactttacta
    23761 ccggaaaggt cttttcgtcg gaagctaccc acaaatagcg aaccccgttg ccagttaggt
    23821 aaatacggtt tataaattgg ttatacaagc ctgcagcaaa ctttaaatcg agctttgatg
    23881 ttgggcttat caccatttgg acacgcggct ctacgtagta ttggtcggta aggttatgtt
    23941 gactcactcg ccccccaatg acaagtgatt tcaacccatt ggttttaaag ttaaattgta
    24001 gccaaccagt attaaccctt gaattaaaat cgtactgtct tagggtatcg gcaacaggaa
    24061 gggagtttac agcatgataa taattctcag atgatttaga attcactaaa ttactctggt
    24121 atcccagctc tagtatgatt tggttcgaaa gagttacctg ggcattcagg tttacagaga
    24181 gatcgtttaa tcccgtttcg gttgcatagt ttcggacaat ggtgtcgtaa accggctgta
    24241 ttccacggcg ttttaatgtg ctgtcgtaat gttgatagtt gcttgtaaat tcagaaatcc
    24301 caacggtaaa attttggtat aaccagctat tccactgctt agcccagcga gcgccagcac
    24361 cactattccc ccaaagagca ttttcattaa taaaaggcga tgataccatt tgcaggaaat
    24421 tgagcctatc gcgtcctaca tacccactta ccgataaaat atcgttgttt gagggtttca
    24481 cgctaacctt tgcgtttgcg tcgtaaaaaa gatacgaggg ggcgtcggga tcggaaccaa
    24541 acgcggtaaa gttctgcttg gaaagcataa gatccgaagc cacactctca aaaagattca
    24601 gaaactgtgg ggaacggtaa aaatcggtat aggatctacg cgccgacact ataaaggagg
    24661 catgtttccc caacggacct tcaaatgtga aatcgccagc aagctggtcg gtgccagcat
    24721 caacacttac actattctcg ttgccggttt tacctgttat ttctattact gacgatgcac
    24781 gaccacccca cctcgaatcg aaaccgccgc ggtaaagcct tacatccttt atggccttac
    24841 tgttcaagct tgagaatgcg ccaaaaagat ggttaatgtg ataaattgta aagccatcgt
    24901 acgagatcag gtttttatcg gctggcgaat ggcgaaccat cagccctgaa agcgattcag
    24961 tagtaccatc tactcccgga aggagctgaa gtggtgccaa aatatcaatt cctgagattc
    25021 ctggtatatc gcctaccttt tttgtgtcaa aaaccatctc tccggggcgg tcgccggtag
    25081 caagcattga ggtaatgttt cgcactactt ccacatcggc caggatgaac tttttgggat
    25141 cgagctcaac aactataggt tttgaattgt actgtacacg agccaccctc atctgtaagg
    25201 gttcaaaacc aagataccga acctctatac taaccgaatc ggattttaca tcgggtatcg
    25261 taaaaaaacc atcggtattg gtagtagctt ctaaatcgtt atccaccact cttattgacg
    25321 cgtaaggcag ggcttcgccc gaaatcctat cgcgtacaat tccctgaatg ggtttgtttt
    25381 tttcttcaac aatcggtttt tctgcccttt taatgacata aacgttattg agtgcaataa
    25441 actcaaaccc tgaatctttt agtaactgct tcaaaacttt atcgggcgaa tcgttcctaa
    25501 actgtccggt aaaggaaatt tggttgagtg ctaccgggtc gtaggcaaat tttatgttgt
    25561 acttacccga caaagctgag agtatttgat ctaaggattg attgctaaaa ctttcggtaa
    25621 tcttaacctt ttgggcataa gtagcttgtt ggatttgtaa agcaaatcca accagaagtg
    25681 atataagtaa atactttatt ctgctcatgg ggtcaggtta tatctcatgc ttaggttaat
    25741 acaacttaat aacccaattc cctacctgaa agtgtaaacg gttgaatcgg cattaacagt
    25801 atagctcaaa cccatagata ttgatattac atcaagtaca tcagttgcct tgcctgctgg
    25861 aatggttcca gaaaagtatc ggcttgccgg atcaaaaccc tcggcgctaa tctgtatatt
    25921 gtagaagttt gatatttgct ggaaaacttg agaaagcggc tcttctgcga actcaaactc
    25981 gtgtgtaacc caaacaggtt tggttaactc cttatcggat tgctttgaat gtattaacat
    26041 cccattggtt ttagttacgc tctcgccctt tttaactcca acgggtttta aacctgaaat
    26101 caaaacattc actgagccct caaagcatcg cacctcaaag gaatcacccc tggttacaag
    26161 gctaaactcc gttcctgtta caaccacctt ctgtttctgg ttatcggtaa ccacgaattt
    26221 tcccttaccg ctctttacct tgaagtaagc ctctccctcc attctaacat ttcggccaaa
    26281 ccaaccatat gattttttat aggaaatcca tgagctggca ttcagatgta ctaccgatcc
    26341 atcgggaagt gtatgttcaa gggtttgtgc ctgcatcgtt tcaaaacggg actcagtatt
    26401 caggataaat aagctgccaa ccagcattag tataataact gatgcggctg caagcaacca
    26461 tttgtaatgg ggttgaaagg ttacaaccct tgcgggttcc gcatgcttac cttcaatggt
    26521 ctgcataagc atgtcccacg cctgctcttt agtacggttt gatggaggaa cagaaatgtt
    26581 aaactcaatg ggattactat caaaccctga gtcagtgcta tttatatttc tgtccctttt
    26641 catattttgt agccaaattt ttgacgaata gctgccagtg cttcggacat gcgcttttca
    26701 attgccttaa cgctaagtcc aagccttgca gcaatttcct cataggttaa cccatcaatt
    26761 cggttcatca gaaaaacttc cctctgtcca tcaggtagcg atgctaaaac ctcttgcaat
    26821 tgcttattga gctgatccat tcttattctc tcatcggcgc tatttggatt tgaggtcata
    26881 tcacctttaa ctgcaacctg aaagctatac accaccttga ggtgcttata gtgattctta
    26941 attatgttgc cagctatggt gtaaagtaac gatttaaccg tatcgttatt gaccttgccg
    27001 cgcatgtccc agagtttaag aaaggtttcc tgcactatgt cgtccgaaag atcaacatcg
    27061 ccggttttat agtaggcaaa atgcctgata ctctcgtagt actgatcaaa aatactacgg
    27121 aaccagctta actcctcctt ggtgtagttt ccgcttggta aggtcatttg aatattgtgg
    27181 agtaaagcaa aaatacattt ttttacattc aatcagctga ccatgtaaat aaaaaagcaa
    27241 catccttaaa tggatgttgc tttcggtatt gaccttaaaa aatactagaa atcgctgttc
    27301 atgctccaaa ggaattcctc attagtagct gttttcacta gcctatcgcg caggaattcc
    27361 atagcctcaa ccgagttcat atcggtaagg taattgcgga gaatccatat cttctgtagc
    27421 acttctttat caagcagcag atcctcgcgg cgtgtactcg atgccatgat gtctacagca
    27481 gggaataccc tcttattcgc taacttgcga tcgagctgca gctccatgtt accggtgccc
    27541 ttaaactcct caaatataac atcgtccatc ttgctaccgg tttcggttaa ggctgttgcc
    27601 agaatggtta gcgaaccgcc attctcaata tttcgggcag cgccaaagaa acgcttgggc
    27661 ttttgcaatg cattggcatc aaccccaccc gatagcacct tgccggaggc aggctgtact
    27721 gtgttaaaag cccttgccaa cctggtaatt gaatcgagaa gtataaccac atcatggcca
    27781 cactcaacaa ggcgcttggc tttttccaga acaatattgg ctaccctaac gtgcctttcg
    27841 gcaggctcgt caaaggtcga tgctatcacc tcggcgttta cgctacgggc catatcggtt
    27901 acctcctcgg ggcgttcatc aatcaaaaga actatcatgt aaacctccgg gtggtttgcc
    27961 gctattgagt tggcaatgtc tttcagcaat atggttttac cggttttggg ctgggcaaca
    28021 attaaacctc gttgtccctt gccaatgggc gcaaacatat cgacaatccg tgccgatagt
    28081 aaattcatgc cattcccaat cagcttaaac ttctcgtttg ggaaaagtgg ggttaagtaa
    28141 tcgaacggaa ccctatcgcg tataaattca ggatcgcgac catttatctc aattaccttt
    28201 attaatggga aatacttttc gccctccttg ggtgggcgaa tggtgccagt aacggtatcg
    28261 ccggttttaa gcccaaaaag cttgatttgc gattgcgata cgtaaacatc gtcgggagag
    28321 ctaaggtagt tatagtccga tgagcggagg aaaccatacc catcgggcat aatctcaaga
    28381 acacctgtag tggctacaat gccatcaaaa tcaaatcgct cgctaccggg gcgcctttcc
    28441 tggctaggaa tgggttgagc attgttttcc tcctttactg gttcaaccac ttcggtaacc
    28501 gatgcccgtg cctcctgttc cggtttttca gaaaccaagg cctcagcttg agcctcttcg
    28561 gtaaccatag gaattactgt atcctcttcg tcattgctta aaagtatggt ttccgaatgt
    28621 gtttcggtat cgagctcagg gaggataacg gccttttcgt taaatttaat ggtggtttta
    28681 ttgcttcgtt ccttttcttc gcttgagcta ttggtagggg tagctgagaa ctcctttcta
    28741 acctcttttg cccgaataat ccttttacgt ttattccttt catttattgc ttcctggcta
    28801 cttgttgcct cgtcaggctt agcggcttgc ggttcctctt ttttaggtgc aaccggttgg
    28861 gtttcttccc tctttacctc tacctgtttc tcaggctttc ggaaaggttt tcggtgatcc
    28921 tttttccgct catcgtcagc tgaaagcgat gcgtttattg cttgttgatc gagaattttg
    28981 tagattaact cttgcttctt aaagttctct accttcttaa tattcaaatt tttagcaatt
    29041 tcccttaatt cgggaagcaa ctttttgttt agttcaagta tatcgtacat agtttgtaaa
    29101 atggtgtttt gtgtgaaaat gaagatggaa aaaagtaatt gaaattctaa aaccttttga
    29161 tttggattag gaagattttt cctgttttcg atgatgcaaa tttagcttat tgtttgtaac
    29221 atgcaaaata aattgagatt tcaataataa ttggaagaaa ctaactcctt gcttcaaaca
    29281 acacttaaag ataaaatgtt ttgcttaaac aaaaaaacag atgatacaat ttttgtcaac
    29341 tcattgaatt tcattgaatt tttaggtaag ttaaaggaaa aatcaaaaaa gttatagggt
    29401 aaaagctaac tcgtgtatca taggtacaga aacaggtaag caatttatgt taaacttaat
    29461 actttcagcc atgaaaaccc tgaatgcact tattgggaaa agcctgaggt atagcgctac
    29521 agcagttgcg ctgataatct cagcagtgct cacagcacaa gaggtggact acaacctcgt
    29581 aaacggagta attaaggatg ccaaaacaaa ggaacgggtg gtatttgcta ccattagcgt
    29641 gcccggcaca actattggaa cagttgccaa tgccgatggc gaattcaccc ttaaggttcc
    29701 caaaactgtt aaatcgaatg tttttgaaat aactcacctg ggttaccaga acactcaatt
    29761 cccaatagtt aacggaacta gtggcaagga taaggtatac ctgattgaac cttcatcgct
    29821 cgaaattcaa tcggtagtaa ttcgcccaat ggatgccaga actatactgg aaggtgccct
    29881 taagaacatt gacaagaatt acagcgataa acctgttagc tttactgctt tttaccggga
    29941 atccattaag cagaggcgtg aatatatctc cattgccgaa gccgttgctg aaatatacaa
    30001 ggctccttac cggtataccc ttgaaaccga taaagtaaaa attgtaaagg gacgtagggg
    30061 cgaaaatgtt aagaaagccg acaccctgat ggtaaaaatg caaggagggc cacatgtatc
    30121 gctttacctt gatgttataa agaaccccga attaattctc acccgtgaag acttacccta
    30181 ctacaactac gaggtggtgg atgttgttaa gattgacaat gaggccaact acgttatcag
    30241 ctttacacct aacaccaaac ttgaatatcc actttaccaa ggtaaactgt atataaatac
    30301 tgagaatctt gctatcacaa tggctgaatt cagcatcgat ttaagtgaac ccgaaaaggt
    30361 agcccagatt tttgttcgga aaaaacctgc cggtttaatt tttgaaccca tcagcacaaa
    30421 ttacctggtt acatataaaa aggtaaacga taagtatcac gttaactata tgcgtagcga
    30481 ggttaaattc cgtgccgact ggcgtaaacg gatattcaaa accaattaca ccataatgtc
    30541 ggaaatggca ataacggaac ggaactttga caatgttaat aaaatcacat tcaaggagtc
    30601 gtttaagccc aacttgatac tggccgacag ggtaaacgag tactttgatg agaattactg
    30661 gggttcatac aacaccattg aacccgacga gtcgatacaa tcggcaatca gtaagtttaa
    30721 caagcggttc aggaagcagt aaggattgga actgggcagg ggtaagccct gccctttctt
    30781 tgctttgttt tattaatttt gtatttgttc aactgttttg tttatggtat tggcctccac
    30841 taaccccaac gagcctgacc ttaaagagtt cgagaaagta ttccggaaat actacgaacc
    30901 actttgccgc tatgcctatt ccattgtacg cgatgccgat gaggccgagg aaattgttca
    30961 ggagttcttt tacacctttt ggttaaaccg taaccggata tctatcaggc tatcgctaaa
    31021 atcctaccta taccgttcca ttcgaaacag tgccttacgt taccttcagc acaaaaacat
    31081 tgaagaccaa taccgtaccc aggattacgc cctcaatcat gttgaatcgc ctgagcaggt
    31141 tattcaaggg aatgagatgg ccggtataat tgataaaacc ctgaaagggt tacctgaacg
    31201 gacaaggcaa atattcttgc ttaacaggat taacggttta aagtataagg aaattgccga
    31261 tttgcttaag atttcaatta agacagttga ggccgatatg ggaaaggcct tgcttgaact
    31321 aaggttacaa cttaaacgtg caggttttta aagtaagtaa tgcctaatgc caagaaccat
    31381 gaatagtaaa aaatcatacc aaacccatat agcaaaatac cttagcaacg aaatggcttt
    31441 ggctgctaaa atttggtttt acctcaaaaa ccaaattaat cctaaaacaa aacatgaact
    31501 taatacactt aaagtttttt ttaattccat gaaagtgaac gaaaagaata aagatattga
    31561 atttgatacc ggtaaagcct ggcaaaaact tcaggaaagg ataaaaaccg agtcctatac
    31621 cggacaaagc cgcactgcct ttattccaaa cctcaactat tttgctgttg cagcaacact
    31681 actggttatg gtagcattat cgctatcggt atggttttac tacaatccca aaactatcac
    31741 cctagccaac atgacacaaa gcagtaccat tattcgaact ctgcccgatg gtactcagat
    31801 atttttaggg gaaaacacca ttctggaata tcctgtaagg tttgcaggaa aaactcgcac
    31861 cgtgaaactc agtggcgaag catacttcga tgtggctaaa aatggctcaa aaccattcat
    31921 aataaaaacc tcgcatgccg atgtaaaagt agtgggaacc tcgtttaacc ttaaaacatc
    31981 caacaatgaa gttcacctga aagtaaccga agggaaggtt aagattaccc ttgccacaaa
    32041 ccaaaaaagc gcattggtgc aggcaggcga ggctgccatt gccagcaaga acgatattgt
    32101 tacctacacc caaccagcag gcgagaccat aaaatcggcc atgaagcttt tgatgtttca
    32161 ggacgagaca ttagacaata ttgtaagggt aataaacagc acctatggtt caaacataaa
    32221 ggttttaggt gatgacttaa aagatatgag aatttctgtt acttttgata acgacatcag
    32281 cagcattgtg aatatcctgt cggtgtcatt taacctcaaa gtaacacgta gccccgatgg
    32341 aaccatcatc attagtaggt aaaaacataa aagtaaaacc attttggcat gtattccatt
    32401 gggtagtact ttttatgctc ataatttacg ctgttaaagc taatgcttac gaacctgaca
    32461 gcttactacg gcaaaatatt tcaattagca taaggactac aaccctcttt aaagcactaa
    32521 accagatagg cgacaaagct ggctgttttt tcatttatga tagtcgtgat gtagaaagca
    32581 accggcgtgt aaagtcaata gaatggagtg acaaaccgct caatgaccta ctcaaccagg
    32641 ttattggtga taccttagta aagtacaggg ttattggcaa acatatttta ctttatagac
    32701 caaaactatc cgacacccta aaagcacaat cacaagaaca aaacatcaag tatgttcagc
    32761 ttagcggtcg tattttggat aaggaaacgg cacaacctgt agcatttgct actgttggga
    32821 tacccagttt agctattggg acaataagta acctcgatgg ggtatttacc cttagaattc
    32881 caaagcagaa caccgactca agtatagcta tatcgcacat tggttacatt caacgcaaag
    32941 tgccagccgg gcttcttaca gccaaaacct ttgaaatttt tcttgaaccc agttttattc
    33001 ctgtacagga aatttttatt cgaaacattg acgcacgaaa tactgttcgt gaggcggtta
    33061 ataaacgatc acaaaactac ttccaacagg atatctacat tactgcattc taccgcgaag
    33121 gggtattgcg caataaaagg atattgagct actctgaggc atttgccaaa atctttaaat
    33181 catcgtacag ctttgactcc cgatacgatc aggttaagct tatacgtttc aggaagatgg
    33241 aacaggccaa caggcgcgac accctcgatg taaagctcca ggctggggtg aaagcaaccc
    33301 ttgatatcga tatcatgaaa aacctccccg actttttaga tccggaattt acgcagtact
    33361 atgactacac ccgaaccgat attgttgtgt acgacaacaa atcggcttac gctattgggt
    33421 ttaagcagaa acccgaaatt acggaccccc tttttgccgg cttactatat attgatactg
    33481 aaactttaac tctacttgcc gctgagtttg agattaaccc ggacatggtt cagaaaaccg
    33541 caagccaact tgtggtaaaa agagggagag gaatacgcgt tcaaccccaa aagattaagt
    33601 acctggttaa ataccagcct catgatggca aatggtatgt aaaccacgta agaggtgaaa
    33661 tttccctaaa ggtgcaaacc cgtttttggc cctttgccaa tactttcgac ctgttttttg
    33721 agtatgttgc cctacaggtg gagaccctag aggttaagcg cttcccaaag cgcgaaacgc
    33781 tcaaaacaac cgaagtattc tctaacacac aagccgaata cgacccaaac ttnnnnnnnn
    33841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    33901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgctcaaa acaaccgaag tattctctaa
    33961 cacacaagcc gaatacgacc caaacttttg gaatgatctc aactacattg aacctgaaga
    34021 aagcattcta aaggcattag gtagaattac cccaaaaatt gaaatggtta ctgaactcta
    34081 aaagtaaaaa accactcccc cgccaattag attgtatgcc ggatcggtca gtccaatttc
    34141 ggcctcaaga ataaaactca tgtttttacg taaacctata tcaacaccag caggaatcca
    34201 gagcagaaat tgtgcatcgc ccccatcggg gaagttaata tcggtgtcca ggccagttat
    34261 tagcctaacc ccctttgcca aaccaaaagt aaggttcaat gtaccatcga gtccaaaact
    34321 cccaaagcta tgcgctcccc cagccaacga aatattttta ctcagaacaa actcaacatt
    34381 agctccaaag taattttcgg gtccaagtac accggcagtg ataccaacat ctattccctt
    34441 tgtaagcccg taaccaccat gcagaaaaag catgaactca ttactcccat taattagcac
    34501 tgcaggttca atgcctaccg aaaaagtttt gggttttaga gtttgtccgg tattaaacac
    34561 ttgccctaac gacatcatgt tgatggcaac aaatacagat attaaaatgg tctttttcat
    34621 ggttatgtga tttagaagtt aacttaaaga aagcaagaga aaaaacgatt ttggtatttt
    34681 acaaattcag aataagtaac ctgttaaaga tcccttcaac aacttctctt gaaatgcata
    34741 tttgtaacaa agttaaataa aataagattt aaaaaaacaa tgcagcaaaa tattactatc
    34801 tttcgtcgtt atagaaaaca ttaagccatg aacgtagaag ttaaaaccac agcaggccag
    34861 gccatgggga ttgtaggtat agtgcttgca gtaatctcac tcatcctagc ctttatacca
    34921 tgtatcggat tcgttgcctt gttaccagct gcccttgcct taattttcag tattatctca
    34981 attattcaag ccacaaatgg ttatggttca aaagggctag gcattggtgc actcattgtt
    35041 tctgttctat caattttcct agctgtaatg tggctaacaa taatcagtgg tggcttttct
    35101 gttcttaaag atttagaaag gcatccagaa aaaattgaaa tatttgggaa ggaacttgaa
    35161 aaggaactta acgatgccaa tgttcagata agaattgaaa ctgatagcat ggtaaaaaca
    35221 cttaggcaac ttgagggtga gtccgatagc attaaggtga taaccattaa aaagaataaa
    35281 gttagcaagg gggatgattc acagggtgcc cgaattacga ttgataccga ttctgttcaa
    35341 attagaattg aagccactga aaaaaagaaa aagtaacggt tttaactttt acagcatagc
    35401 tgctactccc taccttaaag taaactcagc atttgcgctg attttacttt tggtttttat
    35461 ttactctctg gttttcagcc agaacaacca tcccatacct gcagcactta cgcagctaac
    35521 ggggatagtt cctcccagta aaggtttgtc ggctgcgttc tcgcagattg taaggctcaa
    35581 ctttacaaat gcatacattc tcaaccctta cagtttaaga gtattttcat ttttcctaat
    35641 tcaatttata ctgcgcgtgg gcttaatagt tacagagtta aggtttccta actctgtcac
    35701 aacatctact atagctgttg atatactaat ttcccttact ctacttatct actgctttta
    35761 cccgctaatt gaattcacct ttaaaatatt acttcaactt ctccattagt tcttcaaacg
    35821 taaagccatc ggttgcccta attcctttgg gggtaagttg taactcgctg caatcggcaa
    35881 agtagtcgtg ctggtagata tagatgtact caccttcaag cgactccttc aaaaatgttt
    35941 ccttttcctt caatgtcaca aggggttcca cgtcgtaact cataatccag ggtagaggaa
    36001 tatgtgaacg gaatgggaaa aggtcggcta caaaaaccag ttttcgccct ccgtagctaa
    36061 ttatcggaat aatttgccca cgggtatgcc cgttaaaaag cctaagttca attccgggta
    36121 aaagttctcc ttcatcctca attaaattta atgcgcctaa atcgcgcatg ggtaataggt
    36181 tctcaggtaa gtatgcatct gcttcacgtg gatttgggtt tactgcccat tcccactgag
    36241 ctcgtgacac gtggtaccga gcatttggaa aggttaactt caggttgcca tcactatccc
    36301 gataaacacc tcctccgcaa tggtcgtaat gcaggtgggt taaaaccaca tcggnnnnnn
    36361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    36421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncgataa acacctcctc cgcaatggtc
    36481 gtaatgcagg tgggttaaaa ccacatcggt tatatcggtt ggcttatagc ccctttggcc
    36541 aagtccctca attaaacctt caccgccaaa ggcataaaca tgactcataa acttttcatc
    36601 ctgtttgttg ccaatgccat tatcaaaaag taccaccctg ccgttaacct caacaataag
    36661 cgatttcagg gaaaggggga ttaggttatt atcgtccggg gggtaaaccc tgctccataa
    36721 aacctttgga acaacaccaa acatggcacc accatcaacc ttaaagtttg aaatattaaa
    36781 aagcgacagc ttcatgatag acttttttta tgtgtgatac aaaatatttt gcccaactaa
    36841 cataacattt aggggtataa ctttgttctt taaaaaagta taattcaatt tggttaataa
    36901 tgattaactt tgcagcaaat ttaacggtaa aatgaagaat accgacaaat gttcgttttg
    36961 taatcgttcg cgcaaggaag ttggcttact tattgcaggg ctcgatggat ttatatgcga
    37021 cctttgcgct gagcaggcgt atcttatagt taaggaagaa cttaaaaaga agggagagtt
    37081 taaacttcaa aaaaacaagc tgctcaaacc ccttgaaata aagaaattta ttgatcagta
    37141 tgttattgga caaaacgagg caaaaaaata tctatcggta gctgtttaca atcactacaa
    37201 gcggctcctt caggccgatg ggggcgacga tgtagagatt gaaaaatcaa acattcttct
    37261 ggtaggcgaa accggaaccg ggaaaaccct attagcccga accattgccc gaatgctaca
    37321 tgtccccttc accattgttg atgcaacagt actaaccgaa gcgggttatg taggcgaaga
    37381 tgttgaaagt atcctcacca ggctattgca ggtagccgac tacaatgttg aagcagccga
    37441 aaaggggatt gtgtttattg atgagattga caagattgcc cgtaagggcg ataacccctc
    37501 aataacccgc gatgtttcag gcgagggcgt tcagcaagcc ttacttaaac tgcttgaagg
    37561 ctcaattgtg aatgtgcccc cgcaaggtgg acgaaagcat cctgagcaga agatgattgc
    37621 cgttgatacc aaaaatatcc tattcatttg cggtggcgca tttgagggta ttcagaaaaa
    37681 gattgcccaa agattaaaca ctacagtggt tggttacggc gcaagcaaaa cccgtgaggt
    37741 tattgataag gacaacctcc ttcaatacat cgccccgcaa gacttaagag cctatggcct
    37801 aatacccgaa ataataggac gcttacccgt aatcacatac ctaaacccgc tcgaccgcaa
    37861 ggctttgcgc gacatactta ccgagccaaa gaacgccatc atcaaacagt atatcaaact
    37921 ctttgagtat gatggcattc acctgaaatt cgatgccgag gttcttgatt acattgtaga
    37981 taaggcagta gagtttaaac tgggtgctcg cggcctacgc tcaatttgcg aagccattat
    38041 gattgatgca atgtttgaac tcccaaccag cgaacagaag gagatggttg ttacgctgga
    38101 atatgctcag aataagctgg aaaaatcaaa tcttaaaagg ttaaaagctg cttaatgctt
    38161 aatcctaaca tactcctcaa aggtgtaacc cggccccgtg gtcgggtttc ctttttctga
    38221 gctaaccggt tgccaatcat tcatgtcgat atcggggaaa aatgtatcgc cctcgtattt
    38281 atcgtgcaca cgggtgatgt aaagtttgtt ggccaagggc aaggcttggc ggtaaacctc
    38341 gccaccacct actacaaaaa cttcgtcctc gctttttgtc atttccagtg cggttcgtag
    38401 cgagtttgcc acctcaatcc catcaaccct gaattcagag tttcgagtaa tcactatatt
    38461 tcgtcgtttg ggaagcggtt taccaatgct ttcgaaggtt ttacgtccca taataacact
    38521 gtgccccgat gtgattctct tgaaccgttt caggtcatcg gggatgtgcc agagtaactc
    38581 attccctccg ccaataacac cgttctcagc aattgcaaca ataattgaga ttttcatagg
    38641 cctgtccata actacacagc aatatcaccc ttaatgtgtg ggtgcgggtt ataatcctcc
    38701 agcttgaaat cagcaaactt aaaatcaaaa atcgatttaa cctcagggtt tattaccata
    38761 cgtggtaact ttcggggctc gcgggtcagc tgtagcttaa cctgctcaat gtggtttagg
    38821 tagatatgcg tatcgccaag ggtgtgaaca aactcgccag gttcataccc gcaaacctgg
    38881 gccatcatca tggttagcag ggcataggaa gctatattga aaggcacacc caggaacaca
    38941 tcggcgctgc gctggtaaag ctgacacgaa agcttgccgt gggcaacata gaactggaat
    39001 agaacatgac agggcggtaa ggccatattc tcaatttcac ctacattcca tgccgatacg
    39061 atatggcgcc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    39121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ggtaaggcca
    39181 tattctcaat ttcacctaca ttccatgccg atacgatatg gcgccttgaa tcgggattgt
    39241 ttttgaggga atcaaccacc ttgcttatct gatcaatatg ccttccatcg gctgttggcc
    39301 agctacgcca ttggtaacca taaaccggtc ctaactcacc gttggcatta gcccactcat
    39361 cccatatggt tactccattc tcattcaggt accgaatgtt ggtttcccct ttgaggaacc
    39421 ataacagctc atggattatt gatttcaggt gaagcttttt agtggtaagc aacggaaagc
    39481 catcctgcaa attgaagcgc atctggtaac caaaaacact aatggttcct gttccagttc
    39541 gatcgtcttt acgtacacca tgattaagga tgtgatcgag cagattaagg tattgaacca
    39601 tgaccaaaca tttttctgct caaaaatata aaagaaaaac gaagcgagtg tgaactgttg
    39661 atatcaaatg aaaacttctc cttttgcaga tagataccac ctgcgagccc acactgccag
    39721 ggcagcaata atcagtctta tgccaagcat ccatgaaatt gatacaccaa gggctgcaaa
    39781 caggagcaaa tcctccaggt ttgagtgcga aatggcaata tggtagttaa gcaaatcgga
    39841 ttcggccttt ctaattcttc cctggctaac ctcatcgagc attacagccg aaccgtaggc
    39901 aagccctaac gtgtttgcaa taatccacag gaacgtagtt gactgtggca ggccaagaaa
    39961 tcttattaca ggcgacaccg gagccgttag aacctctatg agattgaact ccctgagcat
    40021 ttcctgcaaa accattaaca aaaaaattag aacgataatt ttaaaaatca tcctaaatgc
    40081 tgagaacagc catccatgca ggtgagcaac aaggcttagg gtttgttcat gattggcagg
    40141 gtgtccaaaa gcctgatcta aaggtggaag agttaggttt aacagaaatg ctgcaacaaa
    40201 tgccataccc aaacgcaaaa tcaccatatg ccatgccttt gaaccagttt gcgactgcac
    40261 agcggtttca acaataagat tgtgcgctat gagactcatt acggcaataa tggtaacctc
    40321 acgtaacgta aaggttaact gcgacaatac aactatcact gagtagatgt tcaagaaaac
    40381 tccggttaca aaagctattg ccgcctcgcc ccgtaaacca atcaacttga atgccggggt
    40441 taatagctcg ctaaacgcat ttagcaagcc aaagtagctc aaaacactta ccaaaaacga
    40501 tatgggcaat gtaatcttta gtatccaaaa agaagttttt aatgcttttg gaaaagctct
    40561 cttgaaacat tctaaaattc tctcggaaat agaaccaatt aaaaacctta aactgactgc
    40621 aatggccaaa agtttgcttc gcatatcaat agctattgtt actatgatgc agtacctatg
    40681 taaaggttta aaaagaataa gcccgggaaa cccgggcttt gtgcggatag agggactcga
    40741 acccccacgc ctttcggcac tagatcctaa gtctagcgcg gctaccaatt acgccatatc
    40801 cgcaaaattt cggtgcaaag ttataaagta tttttaaaac accaataacc tcactttatt
    40861 ttggataatt atttcctaag ttcgaagttt tgccccaggt aaagcttacg aacctgttca
    40921 tcatcggcaa gttcttcggc cgtgccggct ttaaggattt caccatcgta aagcaggtaa
    40981 gccctgtcgg taatggaaag tgtttcatgg acattatggt cagtaataag aatgccgatg
    41041 ttcctatcct ttaggtgggc aacaatgttc tgaatatcct ccacggcaat gggatcaact
    41101 ccggcaaagg gctcgtcgag caggatgaac ttagggtcaa gggcaagtgc acgggctatt
    41161 tcggttctac gcctctctcc acctgaaagc tgtatcccca agctcttccg aattttttgc
    41221 aggccaaact cattgagcag cgactccaac ttctcgtgct ggtactcctt gctaaaattt
    41281 gtcatctcca gcacggcctt aatgttatcc tcaacgctaa gctgcctaaa aaccgaagct
    41341 tcctgagcaa gataacctat tccgcgctgt gcacgacgga acaccggctc gtcggttaaa
    41401 tcaagatcat caaggaaaat tctgccagaa tttggtttaa tcagaccaac tatcatgtag
    41461 aacgttgtgg ttttgccagc tccgttaggg ccaagcaacc ctacaatctc accttgactt
    41521 acctcaacgc ttactccttt aactacagtc cgagtcttat atcgcttaac cagattttgt
    41581 gtgtagagtc gcatagtaca gttaactatt caatttgtgc gtaaaagtaa ctattttatt
    41641 tttcttaaaa tataccttaa tcttttttaa tcgtccagct atccatatac tttcccgcaa
    41701 tccagttcaa ctccatttat ggtataaaaa ttgtaataat gcgattagga atttttttgt
    41761 aactttgcat aagaaattct caaaaatgat aaaccctcca aaacatacac ccnnnnnnnn
    41821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    41881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntgtaactt tgcataagaa attctcaaaa
    41941 atgataaacc ctccaaaaca tacaccccgg tggatagttt ttttaatcga tttggttata
    42001 tgcttatttt ccataacctt ttcgtttttt gtcaggtttg agttttctat ttcagaggaa
    42061 tacagaatac tgctaaaatc ggttatccct ttagttcttt ttatcagagc ggttcacttt
    42121 ttatcgttcc gtacctttgc aggtcttatt cgctacacta gtaccaagga tgccgaaaga
    42181 atttttgttg taacaagcgt aagtactttc gtactttttg tcatcaactt catcaacttt
    42241 tttattagta gccgttttat cattccaaac tccattttac tgattgatta ctttttgacc
    42301 ataatactaa tggttgggtc aagacttttc tacaaaacac tttactacac cattagaaat
    42361 tctgacctaa atagcgaaaa gatattaata gttggggttg aacaatttgc tgcggctgtt
    42421 aagcgcgcta tcgacaggga aagccttaca ggtattcaaa tagttgggtt tattgaccct
    42481 tacaacaagc aggaagggaa taagatagaa aacattgaga tttacaatat cggcaaaatt
    42541 gagtacctga tccagaagca cgacgttgcc aaggttattc ttgcaaccaa gaatcttgat
    42601 ccaaaccgaa aaaacaagat tattgaaaag tgtttaaacc taaacgttag cgttcaaacc
    42661 atccccgatg caaatgcatg gattaacggg gaactcaatg taaagcagat taaaaatatt
    42721 aaaattgagg atttacttga aaggccaccc attgtccttg acaaaaagcg aatacaggac
    42781 tacattaagg gtaaagtggt cttggtaaca ggtgctgccg gatcaattgg tagcgagatg
    42841 gtacggcaaa tcaccaggtt tcgtcccaat aagatcattc ttttcgatgt ggccgaaaca
    42901 cccctatacg aattggagct tgagctgaag gaaacttttc agtttttcga ctttgaaact
    42961 gttattggaa gcgttacaaa cgagtacagg gtgaacaggc tgtttgaggc atttaagcca
    43021 caagtggtat tccatgctgc tgcctacaag catgttccca tgatggagaa taatcctacc
    43081 gaagcagtgt ttaacaacgt tttggggaca aaactcgttg ccgatatagc cgttaaattt
    43141 cacgttgaga aatttgtaat gatttccacc gataaagcgg taaaccccac caatgtgatg
    43201 ggtgcaagca aacgtatagc cgagatatac acccaaacgc tgaactatac ttcccaaaca
    43261 aagtttatta ctacccgttt tggaaatgtt ttgggttcaa acggatcggt cattccacgc
    43321 ttcaagaagc aaattgagaa tgggggacca attaccatta cacatccgga cattatcagg
    43381 tacttcatga ccatccccga ggcttgtcag cttgttcttg aagcgggggc aatggggcaa
    43441 ggcggtgaaa tattcatttt cgacatgggc aagccagtaa agattctcga tctggccaag
    43501 aaaatgatta agctctcagg acttacctat ggtaaggata ttcaaatcag ctttaccgga
    43561 cttagacccg gggaaaaact gtttgaggaa ctacttaaca acaaggaaaa caccatccct
    43621 acctaccacc ctaagattat gattgctaaa gttaacgagt ataagcagga agaggtgatg
    43681 cctaaaataa tggaactatt aaacactctt ccaagccaca ataattttag gatagtaagt
    43741 ataatgaagg atattgtccc cgaattcgta agccgaaact cgattttcga agaaatcgac
    43801 cagcaaaaaa agtacagaaa tgagcagcaa tccaaaactg cataaatttt tccaccgtac
    43861 acccatccaa atcaggttta acgatatcga tcagctttca cacgtaacca actcggttta
    43921 ccagcagtac tttgacctag gcaggttgaa atattttact gaggttctac aggaaaaaat
    43981 gaactgggag atagaggggc tggtactggc gagtatcacc atcgattacc ttattcccat
    44041 acgcatgtgg gataatattg aggtattaag taaggtttat gaaataggca acaaaagcct
    44101 gaaaatgcgg caggagattt ttaacaacac taccggtgaa gtggccgcag taagcaaagc
    44161 tgtaatggtt tgtttcagca atgcggccgg caaatccatt cccatcccgc accgttggag
    44221 ggagttaata ttaagctttg agaaagattt acagctatag gtttaaatca atcaaccctt
    44281 caggaagttc catttcaagc agcttagccg aagtatcaac ctccacaatc agttcgtcag
    44341 ctgctgggat cagtacatgt ttaccatctg gggtaaccag ctcaaacgtt gcattcatgg
    44401 agtactcagt gtagtcaact attactccaa cctccgattt atcagtattc cgcactttgt
    44461 atccaactaa atcggaaagg taaacttcgt cctccagctc tatactggtg gaagggatta
    44521 acattttaac accgtaaagt tcctgcgcct ggtcaataga gtccacatca tcgaacttta
    44581 caattgcttt ttcagggttt tggaaacgaa tacgggtaat aaaaaaagga accggtattc
    44641 cttcaatttc aagaaatacc gattccaatt cttcggggtt gaactcaacc gatgagttta
    44701 aactcaccgc cagttcaccg ttaatgccat gtgttctttg gatatatcca atctcaacga
    44761 aaccggcatc ccccatgcac cttaagccta agcgttttcg gtttcctctg aagttgattc
    44821 tgcttgagaa tcggcttctt tggcggccat ttcggcgcgc tttttggcta tttcctgagc
    44881 gcgagcatca cgaaccttcg attcggcttc catgcgtttc ttcatctcct cggcttcgcc
    44941 cttggccaat gaatcaatct tagccttaat cttagcttcc ttttggctta cccaatcctg
    45001 gaatttagcc tcagcttgct cgggggtaag agcacccttt ttaacaccct caagaaggtg
    45061 tttctttagc attacacctt tgtacgatag gatagcccta caggtgtcag taggttgagc
    45121 gcctttttgc agccagtcca atgccctatc aaaatttaat tcaatagtag caggatttgt
    45181 aaggggattg tatgaaccaa tcctctcaat gaatctgcca tctcgtggcg ccctgctatc
    45241 cgccaccaca atgtggtaga aaggtctttt cttccttccc tggcggctta gtctgatttt
    45301 tacaggcata ctttacgctt ttagttaaac ctactatttt actttagcgg cgcaaagata
    45361 tacttttttt tacgatttgg aactcagaaa attgctttat tcttattttg atataaattt
    45421 ttgacaattg ctatttgaaa aaaaatagta acctttgcag ggtatgggtt tgttaaaaaa
    45481 cactaaacct actaagcatg aaaagagtat caacgattac gcaaaagctg ttagttttct
    45541 tattggtaat actgctatca tcgttagcaa acgctgaaac cattgccaaa aagataaaaa
    45601 ccatcccaca aggtagcgat tcagcttttg ttaccaaaac acttggctcg ccaacagaaa
    45661 aacaggtggt ttccaggtat ttctaccaag ggaatcaggc cgtttttatt gacagcgtta
    45721 taagggatat tcggctagcg gaacctcata aaaaactaaa aattaagctg aaaaggcaac
    45781 ttaaagccga acagaaaaag gaaactccca tatcatcgct caggattgga atgaatattt
    45841 ctgaagcgct ggagcgcgct ggtgaacccg atagtatagt aaagggagag gattggtact
    45901 acaccaacag gcatcgtatt gaaatagcca acggaaaagt tattaaaact gaggttcacc
    45961 taaaagccac acttgagaca cttgattgga tatggctaaa cttcacaaag ggtggtttgc
    46021 tttttatgaa catcaccctg gctttaataa tgtttggtat cgcacttcaa atcaagatag
    46081 agcattttaa gctgttgctg gttagtccta aacctgttat tctcggtttc ctttcacagt
    46141 tccttgcttt acctttgttt acatttctac tagtattaat tattcaacca acaccaagcg
    46201 ttgcaatggg tatgatactt gttgcagctt gtccgggagg aaatatatct aactttattt
    46261 catcgttggc aaagggaaac gtggcactgt caattacttt gacggccatt gccacccttg
    46321 cggcaatatt tatgacaccc ttcaactttg ccttttgggg aaaactttac tcagcggcct
    46381 cgaacctggt aatacccatt gagattgatg cttgggaaat gattagaact gttctgatac
    46441 tgcttggaat accggttata ctcggtatcg cctttgcccg gttcttccca aatactgcgg
    46501 aacgcatttc aaaaccgttt aagatttttt ccattgtggc atttttgggg tttgttgttg
    46561 ccgcgctggc tgctaacttt gggtacttcc tgaaatatat ccatctgatt gctctaatag
    46621 ttattgtaca taatggatta ggattactgg taggctatac tatcccaagc atttttaagc
    46681 ttagcaagca agaccgaaga actatagcaa ttgaaaccgg tattcagaat tcaggacttg
    46741 gacttgtgct aatctttaac cccaacctat tcgatggact tggcggaatg gcctttatcg
    46801 ctgccttttg gggtatatgg catatagtat cgggacttgg gattgcctat tactggtcaa
    46861 gaaaacccct ctaaatgtat ttttgttttt aatccaacat agctatgccc accaatcgtg
    46921 aacgaattga ggaatggtca aaagcatact ggcttttaag gttttaccaa aactttctgt
    46981 ttaagctcta ctttaaaacc gagattgttg gcatggaaaa gcttccgcca aacactacct
    47041 ttatttttgc ccccaaccac caaaatgcgc taattgatgc gcttgccatg ttaaccctta
    47101 aacatagctg gcagcccatt ttccttgccc gtgccgatat ttttaagaaa ccgctggtat
    47161 caaaaatact tacctttcta aaaatcatgc ccgtttaccg cattcgcgat ggttttgaga
    47221 acctctcact taacgatagt attttcctaa aaaccatgga tataatccgt aaaggaaacg
    47281 gtcttgtaat tctccccgag ggtaaccatg ctggtttcaa aatattacga ccgctaaaga
    47341 agggtattgc tcgcattgcc cttcaaaccg aggatgcttc cgatggctca cttaacattc
    47401 atattgtccc tgttgggctg gactactcaa actatgttag gtataggagc aaattgctca
    47461 tccggattgg tgaacctttc cctgttaaga aataccttga cctttacagg caaaacaaag
    47521 cccttgccta caatgccctg atagatgaac tatcagagcg cattaaagcg gaaatgattc
    47581 atattgaaga tgaacagaac tattacgagt accttttcct aacccagctt ttcccaaaaa
    47641 ggtttatcaa ggagaacaaa ctacccaata ctctaaacca ggcatttgaa ataagcaaaa
    47701 ggttgatttt aaaactcgac agacttaagg aaacggacag gagtaccttc gatgagatta
    47761 ttcagttagc ccatgtgctg agggatcgca tttcagccaa ccggattaaa ccccaagcct
    47821 tgccgctaaa ggtaagtaaa gcttttaacc tgctttggat ggttccactt tttgtcgtat
    47881 tgtcgccttt ggccattttt ggtttcatca accatatcat acctataatt attccctacc
    47941 agctaagtaa gaaatttgag gacgttcagt tccacagttc agtccgccat gctgcgggct
    48001 tgctgttatt gccacttgca tacctaatcc aaacagtaat tgttggactg attctaaaaa
    48061 gcttagcaat ggcattgctt taccttgttt tactacctcc cgcagcagtt attctttact
    48121 actggcgtaa gttgttctat aagattagag gattaaccag tattttgatt tttgcgatga
    48181 ggaatagaaa tgagtttgaa ctcatcaata accatttcaa caggttaatg aatgatatta
    48241 agtcgatatt ttaattcgga atatcttgtt ctgtaatcat tatacctata caacgtcagg
    48301 ttttgctgta ataccaatac atttgaaaat gaatgtaact attcttattt ttgcacgatg
    48361 aaattttaac ccgatgctta aggccctaat tgttgacgat gagattgcct cgttacgtac
    48421 gcttgagctg ctcctaggac attttgagca gcatattgag attattggtg ttgcttattc
    48481 tgccagtgaa ggtatcgata aggtaaccaa atatgatccc gatatcgttt tcctcgacat
    48541 cgaaatgcca ggaggtagcg gcttcgattt ccttgagggt tgtcctcacc ggaactttga
    48601 tgttatcttt ataaccgcat acaataacta cgccatcaag gcctttaagt acagcgctat
    48661 cgatttttta ctaaagccaa ttgaggttga tgaactccac gaagcgttgg aaaaggttat
    48721 ccgtcgccgt tactcaaact ttgatggccg taacaagtat ttcgccctct ttgagaatat
    48781 caaggagatt attccccaga agttggtggt ttcaacaaat gacaagtacg agtgccttga
    48841 tgttggcgaa attgtttttg ttgagagtgt tcctaacgga acccaaatca agctggtaaa
    48901 tggcagccat tttctggcta acaacccttt tggcgacatt tgcactttgc ttgaggaacg
    48961 taattttagc ttcatcaacc caagctgcct gattaacctt ggaatgatcc gcaagatttc
    49021 aaataagcca gaaagtaata tagagctgaa aaaccacgag ctaattaccg taaggggcga
    49081 gtatctggaa cgattgaatc atcagctcga gaatatttca aaataagctg ctgttttggg
    49141 tagaattgtt gcacttgata ttggtcgtaa gcgaacgggc atagccatga ccgatccgtt
    49201 gcgaattatt gcatcgccgc tggaaaccat acccacccat aaggtaattg attatcttac
    49261 tgaacttttt ctgaaggaaa acatcgacat actggtagtg ggcaatcccc gccaaatgaa
    49321 caatacccct tccgaagctg ttaactacat aaagcccgtt attaacgcca tcaaaaagag
    49381 gtttcccgaa atgcccattg aacttgtgga cgaaaggttt acctccaaaa tggccttgca
    49441 ggccatgatt gatggtggca tgaagaagaa ggacaggcag gaaaagagca atgtagataa
    49501 aatcagcgct gccattatcc tgcaaagcta catggaggga ttaaaatata agtagtaatt
    49561 ttacgcgtta attaggacag atgatacgaa cagtttacgt ttacggatcg ccagtattgc
    49621 gcaaggtggc tcagaatata acacccgatt acccccagct cgatgcactt atagctgaca
    49681 tgttcgaaac catggagcaa tccgatggta ttggtttggc tgccccccaa attggccttt
    49741 ccattaggct ttttgtggtt gacggcaccc cccttgccga cgacgaccca acccttaagg
    49801 atttccgcaa ggccttcata aacgcccaga ttactgaacg gtttggcgag aaagtaattt
    49861 ataacgaagg ttgcttaagc ctacccggct tacatgagga tgttgaacgg gagtcgaagg
    49921 ttcgcattcg ataccacgac gagaaatttg tggagcacga ggaagtgtat gagggcatgg
    49981 ctgcccgcat catccagcat gagtacgacc acctggatgg catactcttt accgaccgtg
    50041 tatcgcccat ccgcaagcag ctgctccgct caaagctcaa tgcaatagcc aagggcaagt
    50101 atagcgcaag ctacaagtgc cggattggtt aaatgataca tgagtttttg actgctaaat
    50161 ttttcttggc aactaaaact cttatcgtat ccctttgaga gcttgaaaat tcaaatgttg
    50221 ttcagctagc aaaaaacgct cttttgccaa cctgatccca gaaataccgg gataaacttt
    50281 gcggaggaat ctcatattaa gtgtacagtg ttccgtgtgc agtgtacagt gggggtgatg
    50341 tctaaggata aaggataaag gataaaggat aaaggataaa aatttaaaat ttcagatttc
    50401 agatttcaga ttcatgactg aaggtggttt cttaagccat caactatcaa ccatcaacta
    50461 tcaactatca actatcaacc atcaactatc aaccatcaac tatcaaccat caac
//
LOCUS       DAOSAL010000011        49236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_537_length_49236_cov_54.832700, whole genome shotgun sequence.
ACCESSION   DAOSAL010000011 DAOSAL010000000
VERSION     DAOSAL010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 49236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49236
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_537_length_49236_cov_54.832700"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            560..2329
                     /gene="sppA"
                     /locus_tag="PK049_02905"
     CDS             560..2329
                     /gene="sppA"
                     /locus_tag="PK049_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOK60536.1"
                     /translation="MKQFFKYVLATIVGIVISSIVIFLMFLGIVGAIVSSADKKEVKI
                     EPNSILYVDLKQEIVDRGSEDPFKGFDFASFQPNSPIGLNQILKAIANAKEDSNIKGI
                     LLELDAVNAGAATTEEIRNALIDFKSSGKFIYAYSDTYSQKAYYLASVADSVFLNPEG
                     MIEWLGLRSEIMFFKKTLEKLGVEPQILRHGKFKSAVEPFMLDKMSPENREQTLTYVS
                     SIWNQWVKGISETRGISADKLNSLANNMEIFNAKKALENKLVDGLIYKDELIATLKTK
                     LGIEEKKDISSIELKKYFKAPEVAKRKFSKDKIAVIYASGEIGMGEGGDDNIGSEGLS
                     RTIRKARRDSSIKAIVFRINSPGGSALASEVIWREVDLAAKVKPVIVSMGDVAASGGY
                     YIAAPATTIMANPTTITGSIGVFGLFFNLQKALDNKLGITVDVVKTNDHADFFSVFRP
                     MTAEEKAVGQMFIEQTYQTFIGHVSAGRGIPVEQVDAIGQGRVWSGVNAKDIKLVDEF
                     GGLTDAIKLAAEKAGLENYRLVELPKQKKLFEQLMEDMSAEAKAWMLKDELGLSFKAY
                     NRIKSMLNNQGVQARMPYDIEVY"
     gene            2582..2746
                     /locus_tag="PK049_02910"
     CDS             2582..2746
                     /locus_tag="PK049_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60537.1"
                     /translation="MRRNVVIIGAAGRDFHNFNTFFRDKEEYNVVAFTAAQIPDIDGR
                     KYPAELAGKL"
     gene            2855..3919
                     /locus_tag="PK049_02915"
     CDS             2855..3919
                     /locus_tag="PK049_02915"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOK60538.1"
                     /translation="MRLSSIVNAAGANFMLLGPNETMLKSTKPVIAVVATRTGCGKSQ
                     TSRKVIEYLMGLGLKVVAVRHPMPYGDLVAQKVQRFATVDDLKKHKCTVEEMEEYEPH
                     VVRGNVIYAGVDYEAILREAEKDPSGCDVILWDGGNNDFAFFKPDLTITVVDPHRAGN
                     EVSYYPGEVNLRLADVVVINKMDSASPEGIQIVRENIRKTNPKAIVVDGASPIKVDKP
                     ELIDGKRCLIVEDGPTLTHGEMKIGAGTIAARKFGASEEVDARPYLVGKLKETYRIYP
                     NIGRVLPAMGYGEQQLKDLEATINNTDCDTVIIGTPIDLSRIINIKKPTTRVFYDLAE
                     IGLPDLKGVLEKFVKTHNLK"
     gene            complement(4226..5248)
                     /gene="rlmN"
                     /locus_tag="PK049_02920"
     CDS             complement(4226..5248)
                     /gene="rlmN"
                     /locus_tag="PK049_02920"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014709730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOK60539.1"
                     /translation="MVPLYGKTLDELKSIVTAEGLPSFTASQIADWLYHHHADSIDEM
                     TNLSKKAREMLSAKYTVGLSKPERVSKSADGTKKYLFPVANNRFVEAAYIPDDDRATL
                     CVSSQSGCKMGCLFCMTAKQGFQGNLSAGEILNQLRSIPEFKNVTNIVYMGMGEPLDN
                     LENVLKSLEILTSKWGMGWSTKRITVSTIGLIPAMKQFLEKSNCHLAISLHTPFDDER
                     RMLMPIQNVYPIADVVDELKKYRFSDHHRVSFEYIMFDGLNDTPRHVKELVKLLNGIN
                     CRINLIRFHPIPDSPLRTSPDERIIQFRDSLTAKGIFTTIRASRGQDIQAACGLLSTK
                     ALQEKR"
     gene            complement(5252..5917)
                     /locus_tag="PK049_02925"
     CDS             complement(5252..5917)
                     /locus_tag="PK049_02925"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60540.1"
                     /translation="MKSWNEIPYDIQRVISNTAVAPGAYILKFKKNNPFIAGQVLALG
                     VSKEIEPRYYSIASAENDELISILYTERVEGKLTPMLSLLKPGENIMVSKPFGTFTHV
                     KPSPVFIAAGTGIAPFMSMLLTGKASGVTLIHGAPYPEYFYYSDTLISRLGENYIRCC
                     SRCQPGCLFVGRVTDFVKQWNDLDVNRPYYLCGSAEMVVETRDILISRGVPVSNINSE
                     IYF"
     gene            complement(5976..6632)
                     /locus_tag="PK049_02930"
     CDS             complement(5976..6632)
                     /locus_tag="PK049_02930"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOK60541.1"
                     /translation="MIKKSLTVLLTIFALSAYSQTYVGVKGGYTMSDIAFSPVQNTRM
                     LFADGFDYGLVFKYFNLKYFGFQSELYMVNRGYRKPIDLAELGDTLYKRVNTYIELPI
                     FMQVRFNLKLYMIHFNAGPYIAYLVQAREGDNSSGEYQMSNITINPLRDNRIDYGLIG
                     GIGLSRDFKWGTFFAEGRVGYGYGDMQKHTYTGMPKQSKAVFQSVNVGYLYHFGNNVK
                     "
     gene            complement(6643..6945)
                     /locus_tag="PK049_02935"
     CDS             complement(6643..6945)
                     /locus_tag="PK049_02935"
                     /inference="COORDINATES: protein motif:HMM:NF014022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HOK60542.1"
                     /translation="MSVLMNFAMFPTDKGASVSPYVAKIIELIGGLGYEFRLTAMGTI
                     VETPTLQQALDVVDKAYKLLESDCERVYVSINLDIRKSDLGRIDGKVKSVTKKLKK"
     gene            complement(6949..8661)
                     /locus_tag="PK049_02940"
     CDS             complement(6949..8661)
                     /locus_tag="PK049_02940"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOK60543.1"
                     /translation="MKRILITVGASVLVLLMFPRCAKVVSPTGGPKDTIPPVLVNSNP
                     KLNATGFSGQKIVLEFDEFIQLKELQQKLLVSPPLKSKPQPKQKGKKLELELTDTLKP
                     NTTYTFYFSDAVRDNNEGNPIKNFIFSFSTGQTIDTLTLSGRVLNAITNEPVENALVM
                     LYDSFTDTLPYTTLPNHIAKTDKDGKYTLVNLKPIKYKLVVVDDKNGNYLYNQGSESI
                     GFISYEVNPSDKNPKSISIRLFREELPNQIVTGYDRPERNILSLGFSRKPVGGFSLKP
                     VDRLKLKNWYVVEPDNNGDTLKLWIVNDEIANRDTLMAILEYQKTDSLYRLKPQTDTL
                     RFLYYKEEVQQSKSKGKKDEAVQVKKGFTVSTSLPSDRMVIPNVPFELTLPIPIRKAD
                     NTKISIFNTTDSIADEQIKLKVDSLNPRIFRFEKEWKPNTSYKLTILPGTFEDYTGQT
                     NDTLMLEMIGADPEKYGTLRINLTGVSKSAVVELLSEKGIPVDARTIQGNGIVEFSFV
                     KPGKYRLRFIDDSNSNGKWDSGSYMKRIQPERVIYFTDEKTKGVINIRANWDSELQFS
                     LKQE"
     gene            8793..9746
                     /locus_tag="PK049_02945"
     CDS             8793..9746
                     /locus_tag="PK049_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007762335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HOK60544.1"
                     /translation="MNLLFIILGVVILVLGATWLVEGASSIAKRLGISALTIGLTVVA
                     FGTSAPELSVNLISAIEGNSDIALGNILGSNIFNTFMILGLAALVKPIGVQHSTVKVE
                     IPFFLLSALVLWVLSNEVFLDSAELNVISRSAGLVLLLLFCVFMYYNFLVGKEDPASA
                     SPEIETRKLWMSVLMVIWGLAFLVGGGKLIVLGAVNMATKLGVSQSIIGLTIVAAGTS
                     LPELATSVVAAYRNKPDIAIGNVVGSNIFNILLILGLTAGINPIKLYSGANLDLALNI
                     AAGILMLVFAVSGPGRKFDRREGVILVMVYMAYLAYQIIKA"
     gene            9753..10172
                     /locus_tag="PK049_02950"
     CDS             9753..10172
                     /locus_tag="PK049_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60545.1"
                     /translation="MNQQAKEHILHFWTRNLVEKPGAFSFNLFLFLSFGILYSFRILQ
                     SPFILLVFGIITPVILTICLYHMSGVSLQHLLPKVFHKKTSRVFLALLDCSIITLLGI
                     LIYRGILNFFFFRFLQTVMLPILYLIMLRVLLLSEHN"
     gene            complement(10169..11827)
                     /locus_tag="PK049_02955"
     CDS             complement(10169..11827)
                     /locus_tag="PK049_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOK60546.1"
                     /translation="MVKYNYVKEFVEKPIRKHWELNSLSDFKGETFTYADVARKIHYL
                     HKLYQIWGVKTGDKVALVGRNNAEWGIVYLSVISYGAVIVPILPDFSPTEIHNIVNHS
                     DSVLLFAGDMVWGGLNQAEMPNLKAIFSISGFNLLVSNDEESRRLFEAAKSSITDEMK
                     SLTPDKVNYPEVPNSELGVISYTSGTTGFSKGVMLPLNSLAANTDYARNNMHLVKGDQ
                     IVSFLPLAHAFGCAFEFLFPFAMGCHITFLTKIPSPPIILEAFAKVRPRLVLTVPLEI
                     EKIFKKQVQPKIEKPAMKVLLNIPLLNKVIFKKINEKLSNAFGGNFHEIVIGGAALNP
                     DVERFLRRIKFKYTVGYGMTECGPLISYSGWRTNPMGSCGKVINYLEMKIDSSDPYSE
                     VGEILVRGENVMMGYYKNPEATQKALDNGWLHTGDLGVYDSKGFIYIKGRSKSMILGP
                     SGQNIYPEEIEARLNNIPFVQESLVIEQKGKLIGLIYPDYESVQQQGIDPDKLEPVLE
                     HHRLEVNKHLPQYSQIAKLVVHNQEFEKTPKKSIKRFKYQYSDN"
     gene            12448..13131
                     /locus_tag="PK049_02960"
     CDS             12448..13131
                     /locus_tag="PK049_02960"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOK60547.1"
                     /translation="MSTNRFAVIVAGGSGQRMGSEAPKQFLILNGKPILMHTIELFLS
                     LPQKPGIVLVLPKNQMERWKNLCVEYNFTANLTLTAGGETRFQSVKNGLSLIPLNNSL
                     VAIHDGVRPLVSKQVIEECYATAAQKGIAIPVIKPVESVRLVEGSTSKAFDRNSVLLV
                     QTPQVFKSELIKECYNVPEQPYFTDDASVVESQGYTIHTVEGNRENIKITTPFDLQIA
                     EMLIRSKPI"
     gene            13137..14312
                     /locus_tag="PK049_02965"
     CDS             13137..14312
                     /locus_tag="PK049_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60548.1"
                     /translation="MANFKLVEVNSKHTRNDFLELPLSLYSDYPNWIRPLDMDIENVF
                     NPEVNKHFRHGEAIRWVLYDNGKPVGRIAAFYDKEIAKNSEQPTGGIGFFECINSQEA
                     ANLMFDTAREWLKSKGMEAMDGPINFGDRDRWWGLLVDGDFPPNYCMDYHLPYYRNLF
                     ENYGFKVYFHQYTYHRLVNSENVDPVVWEKAERIARNPSYTITTISKKNLKKFAEDFR
                     TIYNNAWGRYTGVKKITEAHAMALMKTMKPIIDPELMYFAYYEGEPIGFFIMVPDLNQ
                     VVRHLNGKFDILSKLYFLYLLKVKKVCTKAIGLIFGIVPKHQGKGVEGALVNEFAKRA
                     LAPGFRYTELELNWIGDFNPTMRRVAEQIGARVRKTHITYRYMFDPTKEVVPPRRVS"
     gene            14380..14808
                     /locus_tag="PK049_02970"
     CDS             14380..14808
                     /locus_tag="PK049_02970"
                     /inference="COORDINATES: protein motif:HMM:NF020558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1896 family protein"
                     /protein_id="HOK60549.1"
                     /translation="METKENFRKYLDEYMVDFFPEIKENMGIEYEDFLRAKTEMAFRL
                     YNDMIERGEMPLMAELGAIEVLKEGYTNSKYSYIEERIREMDEDYYERLESEGTLKKK
                     IIEIVKAGEGIFEKYGFEKIDFAYTDEFEDEMMEVIKLKL"
     gene            14812..14964
                     /locus_tag="PK049_02975"
     CDS             14812..14964
                     /locus_tag="PK049_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60550.1"
                     /translation="MSYNKRQVFEDNLEAIRTYYDITGNEREKTKEDIEKISKYKGFG
                     GLKVVL"
     gene            15172..17454
                     /locus_tag="PK049_02980"
     CDS             15172..17454
                     /locus_tag="PK049_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60551.1"
                     /translation="MNTKSIAFIGLLWLSALLAFGQRDIEPRLLFSDTASFNFTGEWQ
                     YLTTDIFLLNGDKFSTLINELDFARVEPKKKRRKKLGVEEEQLEYLFITASLKNVKFF
                     GDNDITYPLYNFQISRDKDSKYQTFVSDNIDYVRIIDNLPLYSARDYIDAEIRVRAIT
                     NNDRDQMLALVASQLKNLSKITTPTDAVMSIIGEFGNFIEANTKKKEYRFSSTIRLFE
                     QKNFDTRLHSIRLYLLSTANTPALEFSGVPLRQFLDTVTNGRVNRNQLRELIGIRSYP
                     VIVVANYKSLYRTEQISGDEVTFANIEKRKLKVENDFRQGLINAETYRQEKDLLNFLN
                     IFAQLKNHLDIYSLNYRTGNNDAISVSLFRVMQYYRQLQKAYEEMKYKYRGNNTFSTV
                     FNQEYESILGFASLYMDNDHNLKSTKNLVNTLVRLEADPKVPNADLEKYIADLRFSDI
                     FKPELMSQNLEGQIIANHLSRLEEQLFKGTFEAEIERVRKAEANPKNKTITDNLLRLA
                     KSTSCSLCRERSLGAVTEFTQRLDAYYLKVELQRYDSIATALQPWVFNRIELIQLMQQ
                     NFKHLYPDNANLESARFLNGKITEIERDVFNIRDFLKIDLSGKDLSMVKNFNEKLLAI
                     QRQADANIDLICKLRPELCSRQQAATQNMKNTELSNLFTRSDSVARQTEVFYSIFQFQ
                     LNRLARVPETSKNDELRIETERLAHQLDELKVAISLLDGKNITQETYSKLVKQVNQQV
                     KDISDKLVTLNEKVSLSSNP"
     gene            19107..19397
                     /locus_tag="PK049_02985"
     CDS             19107..19397
                     /locus_tag="PK049_02985"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOK60552.1"
                     /translation="MLNRDDILNKLRELKPFLKREYSVKEIGLFGSFASDSARDDSDI
                     DLLIELEKPIGWKFFSLEIYLEQIFGRKIDLVTKNALRDRLKEDILNKVNYV"
     gene            19399..19755
                     /locus_tag="PK049_02990"
     CDS             19399..19755
                     /locus_tag="PK049_02990"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOK60553.1"
                     /translation="MDKDKRTYLMYLEDIQLSISRISEYIAGMDFISFKRDYKTIDAV
                     IRNFEIIGEASKNLPDFVKEKYPKIPWREMYYLRNKVSHEYFGIDYEIIWDIATNYLP
                     ENKLLIEQIILEESRK"
     gene            complement(20081..21298)
                     /locus_tag="PK049_02995"
     CDS             complement(20081..21298)
                     /locus_tag="PK049_02995"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOK60554.1"
                     /translation="MIFESYAVAMVNPKTIDMKKFSSFIIAVLAGTVTHAQSASESIN
                     TFAFDLYRGLKVGGNENLVYSPFSITPAFGMVTLGARGETLNQLNATFHFGNSPKFHQ
                     AVGKLQQSLIKSSSADITINVANKAWLQTDYKILKRYRCDLKGNYKTSLYKVDFINDP
                     EAARKTINHAVEYDTQRHIKDLLPQGSISNLTRLVLTNAVYFKGKWKETFEPEKTKER
                     DFTLADGSKTKHQFMSADKTFGYLKGTDFAALEMDYKGEELSMLLILPNEGKPLSEFE
                     KAFTVEKYNEVVKAIEPQKTLVFIPRFTVESGFSMKKVLGEMGLTIPFSDDADFSGIS
                     GNKDLKISDAYHKAFIEVSEEGTTAAAATAVVVAMKSMPNFNVFDANRPFMFILRHKP
                     TSTILFIGRVAKP"
     gene            21523..22452
                     /gene="gldA"
                     /locus_tag="PK049_03000"
     CDS             21523..22452
                     /gene="gldA"
                     /locus_tag="PK049_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     ATP-binding subunit GldA"
                     /protein_id="HOK60555.1"
                     /translation="MSITVNSITKQFGKQVALNSVTFQVPQGQVVGLLGPNGAGKSTL
                     MKIITGYLTPDSGSALVNGVDINSPAYDFRRDIGYLPEHNPLYTDMYVKEYLLWVAGI
                     YRLGKNARKRVLEIIDITGIGHEQNKKIGSLSKGYRQRVGLAQALIHNPSVLILDEPT
                     SGLDPNQIVEIRNLIAAVGQNKTVILSTHIMQEVEAICGRIIIINKGSIVADGPASEI
                     KALTSSSVQNVVVEFLEAPSVELLQSIENVSNVNQLTATTFLVSSSSNTDIRPTIFNF
                     AKNNNLTLLTLQQKVLNLEDVFQNLTLTEPNNR"
     gene            22483..23367
                     /locus_tag="PK049_03005"
     CDS             22483..23367
                     /locus_tag="PK049_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60556.1"
                     /translation="MESTKSSKAVSFLSVLIVILLVVIAGLSYVYYQQKKEAKETETM
                     LLAEKDSLSNNLLNLMNDYKDLETDNDSLNRKLQMEQERAQKLYSELQNLKRVSYAKI
                     KEYQKELGTLRAIMKNMVREIDSLNTLNQQLIAENIKVKEEAATAKKTVKELEQRTEE
                     LNTQVAKGSVVKARNVTAVALNRRGSETSRASRTAKIKTCFTLNENAIAKPGSRYVYM
                     RVVGPDDFVLAKSETDLFDFEGEKIVFSANREVDYQNKDVDMCIFFDSSGELAKGTYK
                     ITLYMDGYMIGYTELVLK"
     gene            complement(23430..23828)
                     /locus_tag="PK049_03010"
     CDS             complement(23430..23828)
                     /locus_tag="PK049_03010"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOK60557.1"
                     /translation="MLAKTYQLIVKYKNSLMWALVILILCGMPPSGLPTIKIPGIDKM
                     VHFGLFAVFALLLISEMNYQRVQLTVKNRHKWVGFIVASVYGGLIELTQQYLFTSRGA
                     EWADFFADILGAAVAVLVYPYINKTLKGYI"
     gene            complement(23835..24563)
                     /locus_tag="PK049_03015"
     CDS             complement(23835..24563)
                     /locus_tag="PK049_03015"
                     /inference="COORDINATES: protein motif:HMM:NF015527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family 2 domain-containing
                     protein"
                     /protein_id="HOK60558.1"
                     /translation="MIVSHRHRFIFIKSAKTASTSVELMLSKVCGPDDVLTPLAPFDE
                     RFDADYYEYQPRNCEGFQNHMSAIAIKKKVGEEVWNQYLKITIVRNPWDMVVSRYHWE
                     KARLWPSIGKNVANILREPFAIVRYKRLLHRIKLIFVLSSFSNFIRYFDKTWTNSRFY
                     FDEEGKPICDVYLKFENLNKGINDLEKMLGIQLGDLPRLKSKPRKGKKHYSQYFNKQQ
                     KERVEKLFKHEIEVFGYTFEQVNQ"
     gene            24757..26145
                     /locus_tag="PK049_03020"
     CDS             24757..26145
                     /locus_tag="PK049_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOK60559.1"
                     /translation="MFEKQVYVERRLKLRKKIKSGLVIILGNTESPMNYKSNTYRFRQ
                     DSNFLYFFGLDIPGLVGVIDIEDGKDCLYGNDFDIDDIIWMGPQPTLKELGEKVGILS
                     THPFKELYSTLSRAIKHGRRIHFLPPYRADNVLLLEQLLGINQSVIKNYSSVELIKAI
                     VSLRSVKEDVEIAEIEKACEVGYQMHVTAMRMAKPGISEQAITGAIEGVAIAQGYMSS
                     FPIILSQNGETLHNHDHSQMLTSGRLLLVDSGAEISSHYASDFTRTIPVDGKFTEKQK
                     EIYNIVLAANNKAIEISKPGIPYMDVHKAAARVIAEGLTKLGLMKGNPDDAVANGAHA
                     LFFPHGLGHMMGLDVHDMEDLGENYVGYDDEITRADQFGIAYLRLGRRLQKGFVLTVE
                     PGIYFIPALIQKWKSEKINESFINYAKVEEYIGFGGIRIEDDILITENGSRILGKRIP
                     VTVEEIEALMKH"
     gene            complement(26200..27297)
                     /locus_tag="PK049_03025"
     CDS             complement(26200..27297)
                     /locus_tag="PK049_03025"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOK60560.1"
                     /translation="MKSNCLGMFVFMAIGLICGHSGWGSNTDTLCVDKKSNDNNYFVV
                     VPVAFYGEETNWGGGLSGGYYFRCRDNKVSSVQGTAVYTLKNQASLWISPRIYTTDRE
                     HYYSGHIRVNHFPNKFWGVGRNTPDTLEEKYVSDDYSVLIQHQRILFGVIMAGVQGQL
                     NYYRTGDYPGDRLLATGISGVNERLTTGLGVLLTWENRENLYYPSFGEFYKASLMVYS
                     KIFGSELNFTKLTIDLRNYYNIYDDHVFALQFLADMTWGKVPFQLMPMIGGADVMRGY
                     YQGRYRDNAMACLQGEYRFPIYKWLKGTIFGGLGDVAPYFDKLDISKSKLTYGAGVRV
                     RVNPIKVNLRFDAAYSDRKEMAYYFTVTEAF"
     gene            27741..28724
                     /gene="nadA"
                     /locus_tag="PK049_03030"
     CDS             27741..28724
                     /gene="nadA"
                     /locus_tag="PK049_03030"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOK60561.1"
                     /translation="MINKESVDKLGYIDEPIDSGINLVNEINRLKVEKNAVILAHYYQ
                     SPEIQDIADFVGDSLALAVKATTLKSKLIIFAGVKFMGETTKILLPDAKIVLPDMNAG
                     CSLADSCNYNDFKSFKEKYPEHIVITYVNTNADIKTLSDICCTSSNAEKVIESIPIEK
                     PIIFAPDRNLGNYLKNKLKRENMLIWDGFCHVHQRFSLERLIELKKKNPNAKIIAHPE
                     CQKTVLIMADYIGSTSQLIDFTINDPSTSYLVATEYGVIHQMKKFSPEKDFIPVPPDN
                     STCACNDCIYMKMITLKKLYLSLKYEYPVVDLSPEIITKAKLPIERMIAIR"
     gene            28724..29722
                     /gene="ruvB"
                     /locus_tag="PK049_03035"
     CDS             28724..29722
                     /gene="ruvB"
                     /locus_tag="PK049_03035"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOK60562.1"
                     /translation="MDSKIIIDKEFEKHIRPVEFNQFNGQNDIVKNLKIFVKAAKLRN
                     EALDHVLLHGPPGLGKTTLANIIANELGVSIKTTSGPVLEKPGDLAGLLTNLEPHDIL
                     FIDEIHRLSPVVEEYLYSAMEDYKIDIIIDKGPSARSIQIELNPFTLIGATTRSGLLT
                     SPLRARFGIKFLLEYYTVDILAGIVKRSASILKIDIRDDAAVEIAMRSRGTPRIANAL
                     LRRVRDFAQVIGNGYIDIDIAKEALNALKIDSRGLDEMDNKILLTIIEKFKGGPVGLN
                     TIATAVGEDAGTIEEVYEPFLIQEGFLKRTPRGREVTDLAYKHLNKLRFPEQGKLF"
     gene            complement(29719..30417)
                     /locus_tag="PK049_03040"
     CDS             complement(29719..30417)
                     /locus_tag="PK049_03040"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOK60563.1"
                     /translation="MLKDGNSNTCISSSFPIFKSLNSEELELLNANLKCNTYRKGEII
                     YREGSRITGIYCVKKGILKIYKTGIDSKPQIVAFAIKGDITGYRSVLSNEMACTTAEI
                     IEEAEICFIPSEIIFNLIKKNSDFALSLIQLTCRELDQANIFIKDIAQKTVKQRLAEI
                     LLMLENTFKLDAEGYIAVNLTREDLSSIVGTATESVIRILGEFKNEGLIAIKGKKIKI
                     NDKKKMNLISDSIF"
     gene            complement(30420..31439)
                     /gene="pheS"
                     /locus_tag="PK049_03045"
     CDS             complement(30420..31439)
                     /gene="pheS"
                     /locus_tag="PK049_03045"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOK60564.1"
                     /translation="MEERIKQIRETFSTLDPKDLQSVEKIRVEYLGRNGYLTKLFDDF
                     KNIPNEKKKEVGKILNQLKSEISDKVETWKKTFSESTKSKGTNLDLSLEEYPFEFGTR
                     HPISLVKNELVEIFKYLGFTVYEGPEIEDDWHVFSALNFPEEHPARDMQDTFFIEKKP
                     DILLRTHTSSVQVRSMENEKLPIRVICPGRVFRNEAISARAHCIFHQIEGLYIDKDVS
                     FADLKQTLLYFAKEMFGSETKIRLRPSYFPFTEPSAEMDVSCKLCGGKGCSVCKYTGW
                     LEVLGCGMVDPNVLELNGIDSKIYSGFAFGMGLERIAMLKYQVRDLRLYFENDVRFLK
                     QFKNY"
     gene            complement(31448..32245)
                     /locus_tag="PK049_03050"
     CDS             complement(31448..32245)
                     /locus_tag="PK049_03050"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOK60565.1"
                     /translation="MKIRTLVLLGLVSMLSCTTNYNDNNRYVKISTDVGDIVIKLYNE
                     TPKHTENFIKLTEENFFDGLIFHRVIKDFVVQGGDPETKNAEPGKLYGEKDAGYLIDA
                     EIVDTIYHKRGVIAMAREGDDVNPEKKSSSSQFYLVVGKVFTEEELNKLEDKLNKKLR
                     EKTEKKLYDSLLGSQSNLNPKFLNEVTQKVSILADSIMKVKHIKFNENQRKIYTTVGG
                     IPHLDGNYTVFGEIVEGYDVVEKISQVQTDSNDRPLKDIKFKVRIVK"
     gene            32302..33774
                     /locus_tag="PK049_03055"
     CDS             32302..33774
                     /locus_tag="PK049_03055"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOK60566.1"
                     /translation="MTFILGKMSSVKQLYSQSIIYGLSTIVPRLLNYLLVPIHTNVLT
                     DPRQYGVITEFYAYISFLLIFLTFGLETGYFRFVNKYKNDERVYSNAFTFVLTLSSIF
                     FIAAYLFSDSISSLLGSGYLPIYIITTAAIVSLDSITALPFAKLRNQNRPLVFSFIKI
                     TGVAINVLLNIIFYLVLKPSYLKNLFPHIDLLFYVFLSNFIQNIVVFVLVTYFTGFPK
                     IRIDKSLMKELIWYSFPILLAGLSGTTNEAFDRLFIKYLLPKSVDSLYQLGIYGACVK
                     LAVLLVLFVQMYRFAAEPFFFKSSGSQDHNKLFGKSFKYFLIFSIVISLLITFNLPIL
                     KFFIGKNYRESLYIVPILLLANIFYGLFFNLSFWYKLADKTIYGLKYTVIGSIITIFS
                     NIILIPIIGIYGAALSRLITYVTMTIMSYSDGRTSGFVSFETKNIKFYIIFILFITLS
                     GTLLLVFLKPYFSLIAANILIGLFIYKFIKKENIKITLWR"
     gene            33765..34202
                     /gene="dut"
                     /locus_tag="PK049_03060"
     CDS             33765..34202
                     /gene="dut"
                     /locus_tag="PK049_03060"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010956168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOK60567.1"
                     /translation="MEIVKILNKSTFDNPTYATEHSSGVDLKAFIEEPINLKPLERKL
                     IPTGLFIELPVGIEAQIRPRSGMALKKGLSILNTPGTIDSDYRGEIGVIVVNLSNDIV
                     TIEPGERICQMVFTRYEKVSFENVNKISETDRGAGGFGSTGHK"
     gene            34202..35386
                     /locus_tag="PK049_03065"
     CDS             34202..35386
                     /locus_tag="PK049_03065"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOK60568.1"
                     /translation="MKVIINLVLVFLFSVNFTFSQNKIDILNNQKKEILKQIDDNNKQ
                     IDIINKSKKATINELTLKLKSIELQNRLLINIKNQITEISKNLTETTSQVDSLSTLID
                     TRRNELIRIYKSYFKKLKRKNDLLILLLSSSSINQAYTRLKMYKNLVNYFNQQISLLN
                     ENKIELEQKRSNYVLFLKELKLKENEYQKTITTLNKEKNELEATKKDLEKKKIELTKE
                     ISNKKKALALIDNEIKRIIEENARALKSMNKENAKRYNELSNIFKENKGKLPPPALNS
                     NVLNYFGESNHPILKNVKVKNNGIDLILNNSSTVQAIHRGEVKKIFNVPYGGRAIIIR
                     HGEYLSVYSNLSEVSVKVGQLVNAGDNLGSVMKQTDNTLVLHFEIWNEKEPENPLDWL
                     KF"
     gene            35462..35857
                     /locus_tag="PK049_03070"
     CDS             35462..35857
                     /locus_tag="PK049_03070"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOK60569.1"
                     /translation="MERTITIPSKLEQISLIEKTIDEITEKLTLTPDVYGKILIAAIE
                     GVNNAIVHGNKLDEKKVVKVNIKICDTKVCITISDQGKGFDPNTIPDPTIPENIENIS
                     GRGVFLMKKLSDEIAFENNGTTVKMTFNI"
     gene            35857..36294
                     /gene="ybeY"
                     /locus_tag="PK049_03075"
     CDS             35857..36294
                     /gene="ybeY"
                     /locus_tag="PK049_03075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOK60570.1"
                     /translation="MAIFFSYKTPSISNRISNKNKIKKWIIYIIEKEFKKSVGDINFY
                     FTDDTEILEINKKYLAHNYYTDIITFNYCTENSISGDIVISVDTVKTNAKDYNVTFEN
                     ELHRVIVHGILHLLGFDDSTEEQKKEIREKENWALKIFYSKWN"
     gene            36285..38141
                     /gene="mnmG"
                     /locus_tag="PK049_03080"
     CDS             36285..38141
                     /gene="mnmG"
                     /locus_tag="PK049_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOK60571.1"
                     /translation="MELAFDVVVVGGGHAGCEAAAIAAKMGSRTLLVSMDLTKLAQMS
                     CNPAIGGIAKGQIVREIDALGGYTAKVTDLTTLHFRMLNQSKGPAMWSPRAQADRMLF
                     SIEWRKYLESIPNLYLWQDEVIDLIVNENRVEGIITKTNQTVKCKTVVLTNGTFLNGK
                     IHVGNVNFTGGRIGELNSQGLTERLVKLGFTTGRMKTGTPVRIDGRTVNYSKLTIQES
                     QELSKFSFNPDYFSKLPRKHCYIVNTNQVVHNILREGFDQSPLFTGKIKGIGPRYCPS
                     IEDKVHTFAEKTSHQLFLEPEGVFTYEMYLNGFSSSLPVDIQYKALKSIPGFENVHIL
                     RPGYAIEYDYFDPTQLKHTLETKIVQNLFFAGQINGTTGYEEAAGQGLIAGANAHLRA
                     NDIQENFQLSRDEAYIGVLIDDLVTKGVDEPYRMFTSRAEYRILIRQDNADERLTPIA
                     AKYNMVDENDVKRLENKIKSIEELNQLVKEYSVSPSDINEYLTSNNSAKITQKRKLYD
                     IVLRPEISLKPTLEHLKINIDSYDYEAINGAEIKIKYSGYIEKEKIIAEKINRLEHIK
                     IPDDFDFNQLNSLSTETRIKLTSIKPKTIAQAKRIPGISPSDINVLLVYFGR"
     gene            38289..39902
                     /gene="ade"
                     /locus_tag="PK049_03085"
     CDS             38289..39902
                     /gene="ade"
                     /locus_tag="PK049_03085"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HOK60572.1"
                     /translation="MKSSNSISGKLVDPIGRRTVNAKIYFQDGVITQIDESLDAENVY
                     IIPGFVDSHVHIESSLLVPTRFAQAAIKNGTVAVVADPHEVANVAGIEGVRFMIENAR
                     QSKLKFFFGAPSCVPASPVDECFEPFTSREVAKLLDLPEVTHLAEMMNFPGVINGNTE
                     VHSILSEAKARNKPIDGHAPGLSGENLTKYVSAGITTDHECFTLEEAKKKIELGMKVL
                     IREGSAAKNFDALHPIIGQNPSMAMFCTDDCHPDELMGGHINKHVRKAIALGYDIFDV
                     LQVASVNPVNHYRLNVGLLQVGQPADFIVVDNLNDFNVLSTYIKGVNVTNFEEDYAVK
                     PPLFSFPNHFDHQKIKLDFPSGKPVKVIKVIEGELITETLKTSVPGSGYDFDKDILKI
                     VVLSRYNPNNIGVGLIHGFGMRKGAIAASIAHDSHHIVAIGANDDSIVRAIDYIIKNR
                     GGICYYDGNETMGLALPFFGLMTNVDVSDAAKEYSLINKKLKADGCKLHAPFMTMAFM
                     SLSVIPHLKLTPAGLFDVDRFSLTSIFAE"
     gene            39908..41704
                     /gene="uvrC"
                     /locus_tag="PK049_03090"
     CDS             39908..41704
                     /gene="uvrC"
                     /locus_tag="PK049_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOK60573.1"
                     /translation="MENLSSISYLSSLPDLPGVYQFFDSTGKIIYIGKAKNLRKRVSS
                     YFNRSQYDSAKVRALVSRVADLKYIVVNSESDALILENILIKKHQPKYNILLRDDKTY
                     PWIVIKNEPFPRVTTTRRYENDGSIYYGPYTSGRLLKALLDLIRQLFPIRTCELNLTQ
                     SNIASGKFKACLEYQIGNCLAPCIGNQKENDYLSNINAIKKILEGDLNEVLRLTTAEM
                     TKAAQELKFEQAALLKERLRLLQNFQAKSSVISKGINTADIFTIQESEGFAVVNYLKV
                     NSGVVVQSYNLQIKNIVDEPIDDIFSQAILEIKERLNLKSTTFLVNINPAYQLSNIKY
                     HIPKQGEKLKLLKLSLRNADAYIKELIKSEERANPQTRALKYLEQLKKDLQLPELPMV
                     IECFDNSNLQGTNPVSACVVFKNGKPAKSEYRHFNVKTVTGPDDFASMREVVYRRYKR
                     LIEEKKNLPNLIVIDGGKGQLSSAYETLKDLHIEHIPIIGIAKRLEEIYRPGDTTPLY
                     LDKRSMSLKLIQNIRDEAHRFGITFHRKKREKKNEASILESIPGVGKKSVIKLYTRFK
                     SLENIKMASLEELSEEVGAKTASNIVSFFKKI"
     gene            42598..43347
                     /locus_tag="PK049_03095"
     CDS             42598..43347
                     /locus_tag="PK049_03095"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016272242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HOK60574.1"
                     /translation="MKIKKIVISQPDPQSEKSPYNELSEKYGVHLDFRPFIHVEGVSS
                     KEFRQQKINILDHSAVIFTSKTGIDHFFRICEELRITVPETMKYFCITESIAHYLQKY
                     IVYRKRKIFFGDNTFKDLLDIILKHKDEKFLLPLSDPHKPEIPKALTKAKINYTKGIF
                     YKTVSSDLSDLKPFPYDMIVFYSPNGIKSLFDNFPDFQQNDIAIGAFGDTTAKAVKDA
                     GLRLDVKAPTDAFKSMTQALDHFLKEQSKKK"
     gene            43384..43764
                     /locus_tag="PK049_03100"
     CDS             43384..43764
                     /locus_tag="PK049_03100"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOK60575.1"
                     /translation="MPQKLRKKYIIHLENEVNSLLKSELSRFHYPIKLIYQVDERIPG
                     GIEENPHYKVIFIIPKRYLKKAYMRNLAKRRLREAFRINQNLLDQFRERGKTVYLAFI
                     YVSAEVVSFKQLESGVVKLLTDLK"
     gene            43766..44026
                     /gene="yidD"
                     /locus_tag="PK049_03105"
     CDS             43766..44026
                     /gene="yidD"
                     /locus_tag="PK049_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOK60576.1"
                     /translation="MNLVKTLKILWKYAVRLLAYIPILLIKIYQIFLSPYLPNSCRFT
                     PTCSAYSVEALKKHGFFKGLWLSVRRISRCHPWGGHGYDPVP"
     gene            complement(44023..45171)
                     /locus_tag="PK049_03110"
     CDS             complement(44023..45171)
                     /locus_tag="PK049_03110"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOK60577.1"
                     /translation="MKILSPRNKFLLTLIIAAVTVSFITIYFTNRFVSELKQEERQKI
                     DLWANAVKYISNEMGSCEDFSLIFEIVQNNRTVPVILTDETGKVIGFRNIELPDIQDT
                     VYVSNLLNKMRAEHDPITIELPDGSKNYVYYFDSITLKKLNYYPYIQLIIIAFFIGIA
                     FYSYGQARKSEQNLIWLGMAKETAHQLGTPTSSLMACSSLLSEKYPNDEVVTELEKDV
                     KRIETITSRFSKIGSSPSLDSIDITNLITDSINYQSKRCPKTIAFNFSPGIPIYAKAN
                     HILLGWVLENILKNAVDAMQGKGVISVEATVEKNNVIIDIQDQGKGIEKGKQKEIFQA
                     GYTTKERGWGLGLSLAKRIVEQYHSGKIFVFRSELGVGSTFRIIIKKA"
     gene            complement(45173..>45711)
                     /locus_tag="PK049_03115"
     CDS             complement(45173..>45711)
                     /locus_tag="PK049_03115"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HOK60578.1"
                     /translation="FSDVQVVQGKLKGMDRIKELSKWLNLDQVKVIAFDTLKNKITIE
                     NRKIFIPNMDIKSNVLLMNVSGVHDFDNRYEYIARINISNLLKRKFVKSDQIDFHSTT
                     DGSINLYLKLFGQNDSYEVEWINKKGFESGIHNTVQTDSVSIQNSPQIKPKEKTDGNV
                     GVGYMLEWDEQIDTLKNE"
     assembly_gap    45712..45811
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<45812..47721)
                     /locus_tag="PK049_03120"
     CDS             complement(<45812..47721)
                     /locus_tag="PK049_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60579.1"
                     /translation="MAILVKIYHRIFRIIILLLLLMLILVLTGAGILFFKQEYIINTL
                     RENLNKYPDLSLTYSSVSVNTLKTFPRFSYSFTDFVLVINLNSKSDTVLKTDDFLISI
                     SPFKLLGGKIDIKNIRVSNARIKWQTNYPEELFGSSDSSSESSVSVLISGISLNNFFF
                     ELLDSVSKTVLYCSGKNLNIKFKTEHTKLLLSIQSELSKTTVGEYFKITNQHQLNFDI
                     ENSDNKLYIYNLESSIKSLRINGSGIYDFNSQLAAFRFKSNRFQAQDLSYLPYLKSFD
                     QIKGIITADAYLRLSSGFEKVDTFQVNYNSNKLKWITKNEETTITNLEGYTLLTNNFS
                     KHFSYIPKASIESNNSKINLNAKIKGFNNLVILAKGYVSHNLRIQKPEIDIETTGQFK
                     ALISYAPKTESITPLLLKSDLVFENKKNISFNQPSVKGTIAINNDLNIAGYFKSDSTD
                     INFNINQANFLESYTQNRYSPNIYLYGNHIDYNDIVHLITDRTSNTSTKGVDLPKMSF
                     MLNFKRATYNRLKLNSLKANGIINGDTISADYFTADCFDGNVSGRFKSHSNSVHTNLW
                     VSNVSIEYVFKTFNNWGQSYITADNLSGRFKGIMNMQFKKNEKGDVDLGSLKLFSDVQ
                     VVQGKLKGMDRI"
     gene            47799..>49236
                     /locus_tag="PK049_03125"
     CDS             47799..>49236
                     /locus_tag="PK049_03125"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOK60580.1"
                     /translation="MMFKKILLPVYAILCLHLVAQPQNADSLLLQLYQESNDSLRVLI
                     LQKIARVYEVNENNPVLAENYLLEAKRLAESIKSNYLRMEVYTSLGVLYRNISNYANA
                     LKFHNDALDLAREIDDKHQQAKSLNNLGVVYRRLDNHAYASEYHLKALKLAEEIKDTF
                     NISVACNSLGNIFSLNGRYDEALEYFQRALYLSQQTNNLLGQAMNFNNIGEVYEFKGD
                     FIKAKDYYSKSLDINYQINSQKGIAINFNALGKIELYLGNPLKAQDYFTKALVIDKKL
                     NDKKFIADSYINLARAQLGLNNDRSAYENVFACLSIAKEIKSLIHFQQAYEVLSDYYQ
                     KQNEYSKALEYFKLSSSYKDSVLNEKNSRHISTIQTLYETEKKENEIKLLLQQQELKQ
                     REITRTKIQTYVLLIGLLLSGAMLLVIYFALRDKRRTAEKLARQLEEIELKNVLLEEK
                     NQEIEQQKQEIEKNKDFIEQKNKNLEEAY"
BASE COUNT        14969 a         9760 c         9445 g        14962 t
ORIGIN      
        1 aatttcgctc tttggaatat catagcgtgc atcgctttac atacgggagt tccatagtgt
       61 tacaaatttt acgcccctgc ggggctgaaa gtggtcttcg ttgttcgttg ttcgttgctc
      121 gttgttcgtg aatgtcatag tgttacactg tgaaacccta tgaggcttat ttagttgatg
      181 gttgacagtt gatggttgtt ggtgagggta atgtcatgtt gagcgttagc gaaaaatcac
      241 tttagggccg attagagttc cttcgctacg ctcaggatga caagctgaga tggcagttta
      301 tggttgatgg gtgatagtga atagaagcat ttcacaaaac actaaaaacc aaacactact
      361 ttccctcatc cctgtacact gcacactgta tactgcacac tattcaatag ttccttcgct
      421 gcgctcagga tgacaggcat gtatatgttt gttaactaaa cgactttgaa ttatctatta
      481 aaaaattcaa cccttttctg taactttatc aacttaaaat agtcttattt aataattgaa
      541 taactaaatc tcaatcacta tgaaacaatt tttcaagtat gtgcttgcca caatagtggg
      601 catagtaatc tcatcaatag taatcttttt gatgttcctt gggattgttg gtgcaattgt
      661 ttcctcggcc gataaaaaag aggttaagat tgagcccaat tcaatccttt acgtcgatct
      721 aaaacaggaa atagttgacc gcggtagcga agaccccttc aagggcttcg attttgcttc
      781 gtttcaaccc aatagcccaa tagggttgaa ccaaatccta aaagccattg ccaatgccaa
      841 ggaggattcc aacatcaagg gtattcttct tgagctcgac gccgttaatg caggcgccgc
      901 caccaccgag gaaatccgta acgcgctcat cgatttcaaa tcgtcgggca agttcatcta
      961 tgcatacagc gatacctaca gccaaaaagc atactacctg gcatcggttg ccgatagtgt
     1021 tttccttaac cctgagggta tgattgagtg gcttggcttg cgaagcgaga taatgttctt
     1081 taagaaaacc ttggaaaagt taggtgttga gccacaaata ctccgccatg gtaaatttaa
     1141 aagcgccgtt gagccattta tgctcgataa gatgagcccc gaaaaccgcg agcaaacgct
     1201 aacctacgtt tcatccattt ggaaccaatg ggttaaggga atttccgaaa cccgaggcat
     1261 atctgccgat aagcttaaca gcttggccaa caacatggag atattcaatg ccaaaaaggc
     1321 gctggaaaat aagctggtcg atggtctaat ctataaggat gagcttattg caaccttaaa
     1381 aaccaagctt ggcattgagg aaaagaagga tatcagcagc attgagctga aaaaatactt
     1441 taaagccccc gaggttgcta aacgcaagtt ctcaaaggat aaaattgccg ttatttatgc
     1501 aagtggcgaa ataggaatgg gtgaaggtgg cgatgataat atcggttccg agggtctttc
     1561 gcgaaccatt cgcaaggcac gccgcgatag ctcgattaaa gccatagttt tccgtatcaa
     1621 ctctcccggc ggtagtgcgt tggcctcaga ggttatatgg cgcgaggttg atttagccgc
     1681 aaaggttaag cctgttattg tttccatggg cgatgtggct gcatcgggtg gatactacat
     1741 tgcagcccct gctacaacca ttatggccaa cccaaccact atcacgggtt ctattggcgt
     1801 attcggcttg ttcttcaacc tgcaaaaagc gctcgataat aaattgggca ttaccgttga
     1861 tgttgttaaa accaacgatc atgccgattt ctttagtgta ttccgtccaa tgaccgctga
     1921 agaaaaggca gttggccaaa tgttcattga gcaaacctac caaaccttta ttggtcatgt
     1981 atccgcaggc cgtggtattc ctgttgaaca ggtcgatgcc attggacaag gcagggtgtg
     2041 gagtggcgtt aacgccaagg acataaagct tgttgatgag tttggtggcc taaccgatgc
     2101 cattaagctt gccgccgaaa aggctgggtt agaaaactac cgccttgttg agctaccaaa
     2161 gcagaaaaaa ctgtttgagc agcttatgga agatatgtcg gccgaagcaa aggcttggat
     2221 gctaaaggac gaactcggtt tgtcgttcaa agcatacaat cgcattaaga gtatgcttaa
     2281 caatcagggc gttcaggctc gtatgcctta cgatatcgag gtttactaat acattcccat
     2341 aacaacattt tgccccgatt ctaaaaaaat cggggcattt ttttccctta atcccaaaaa
     2401 tatgtttctt aacatatttt atgaaaccta cccctattaa actgatattc tgatctttat
     2461 atacaaccat tcatggcggt gagggttgta aaatttttgg gacgaacatt ttgaatttta
     2521 aaaaactaat tttgagaact gtaaaaacca aaatctaaac aaaaatatct aaggaggtct
     2581 tatgagacgt aatgtagtta ttatcggagc agccggaaga gatttccaca acttcaacac
     2641 attcttccgc gataaagagg agtacaatgt agttgctttt accgctgctc aaattcccga
     2701 tatcgatggt cgtaagtatc ctgccgagct tgcaggaaag ctctaaccca acggaattcc
     2761 catagaggcc gaacagaatc tggctaagat tatcaaggag aaaaacgttc acgactgcgt
     2821 ttttgcatat agcgatgtga actaccagca tgtaatgcga cttagctcaa ttgttaacgc
     2881 agcaggtgct aacttcatgc tgcttggtcc caacgaaacc atgttaaaaa gcaccaaacc
     2941 ggttattgcc gttgttgcta cccgtaccgg ttgcggtaag tcgcaaactt cacgtaaggt
     3001 tattgagtat cttatgggac ttggacttaa ggttgttgcc gttcgtcacc caatgcctta
     3061 cggcgatttg gttgcccaaa aagtacagcg ttttgccact gttgacgacc tgaagaaaca
     3121 caaatgtacc gttgaggaga tggaagagta cgaaccacac gtagttcgcg gtaacgtgat
     3181 ttacgctggt gttgactacg aggccattct tcgcgaagcc gaaaaggatc ccagcggttg
     3241 tgatgttatt ctttgggacg gcggtaacaa cgactttgcc ttcttcaagc ccgaccttac
     3301 cataaccgtg gttgacccgc accgtgcagg taacgaggtt agctactacc ccggcgaggt
     3361 taaccttcgc ctggccgatg tggttgttat caacaagatg gattcagctt cgcccgaggg
     3421 tattcagatt gttcgtgaaa acattcgcaa aaccaacccc aaagccattg tggttgacgg
     3481 cgcttcgccc atcaaggttg acaagcccga acttatcgat ggtaagcgtt gcctgattgt
     3541 tgaggacggc ccaaccttaa cccacggcga aatgaagatt ggcgctggta ccattgctgc
     3601 ccgcaaattt ggtgcaagcg aagaggttga tgctcgtcct taccttgttg gtaaactgaa
     3661 ggaaacctac cgtatttacc ccaacattgg ccgtgttctt cctgccatgg gctacggtga
     3721 gcaacagctc aaggacctag aggccaccat caacaacacc gattgcgata ccgttattat
     3781 tggtacccca attgacctca gccgtattat caacatcaag aaacctacca cccgtgtatt
     3841 ctacgatttg gccgagattg gtttacctga cctaaagggt gttcttgaga aatttgttaa
     3901 aacccacaac ctgaaataat tcaggattac aagatagaaa gggcgcctta cggtgccctt
     3961 ttttttgagt tgagagttgt tcgtgagggt attgtcatgt tgagcattag cgaaacatct
     4021 cccttaccat aaaggggaga tccttcaaca ggctcaggat gacaagcgtt gtagttgctg
     4081 ttctacttcg ctttacggct caaggcatac gtttcccgat ttaactcgta attgagattc
     4141 ctcgacgagc tcggaatgac aataggtaat aattagattt cgcttaacga ctcacggtta
     4201 acggttaacg gctcacggtt aacggttaac gtttttcctg tagcgccttg gttgataaca
     4261 atccacaagc agcctgaatg tcctgcccac gcgaggcacg gattgtggta aatatgcctt
     4321 tggcggttag tgaatcgcga aactgtataa tgcgctcgtc gggtgaggtg cggagcggcg
     4381 aatcgggtat gggatggaat cggattagat ttatccggca gtttatccca ttaagcagct
     4441 tcaccagttc ctttacgtgg cgcggggtat cgtttagccc atcaaacatt atatactcaa
     4501 acgacaccct gtggtggtcg ctaaatcggt actttttgag ctcgtcaacc acatcggcta
     4561 ttgggtatac attctggata ggcataagca tccggcgctc atcatcgaac ggagtatgaa
     4621 ggctaatggc taagtggcag tttgactttt caaggaactg cttcatggca gggataagac
     4681 caatggttga aaccgtaatg cgcttggtgc tccagcccat tccccacttt gaggtaagaa
     4741 tttcaaggct tttaagaaca ttctcgaggt tatcgagtgg ttcgcccatc cccatgtaca
     4801 caatgtttgt cacattttta aactcaggga tgctgcgtag ctggttcagt atttcaccag
     4861 ccgaaagatt tccttgaaat ccctgctttg cagtcatgca aaacaggcat cccatcttac
     4921 agcccgattg cgaagaaacg caaagggttg cgcggtcatc atcaggaata taggcagcct
     4981 caacgaagcg gttattggct accggaaaca gatacttttt ggtaccatcg gccgatttcg
     5041 aaaccctttc gggtttggat aaccctacgg tatactttgc tgaaagcatc tccctggcct
     5101 ttttcgatag attggtcatc tcatcgatac tatcggcatg gtgatggtaa agccaatcgg
     5161 ctatttggga tgccgtaaac gacggaagcc cttcggcagt tactatcgat ttaagctcat
     5221 cgagcgtttt tccgtaaagt ggaaccatca gctaaaagta tatctccgaa tttatattgc
     5281 tcacaggaac acctctcgat atcagtatat cgcgggtttc cacaaccatc tcggcactac
     5341 cgcaaaggta gtagggccta ttgacatcca ggtcgttcca ctgtttcaca aagtcagtaa
     5401 cgcgccccac aaacaggcat ccgggctggc aacgtgagca gcatcggatg tagttttcgc
     5461 caagcctact tattagagta tcggagtagt aaaagtattc ggggtagggc gcgccatgta
     5521 tgagggtaac tccgcttgcc ttacccgtaa gcaacatcga catgaatggg gcaattcctg
     5581 ttcctgctgc aatgaatacg ggcgaaggct tgacatgagt aaaggttcca aatggtttgg
     5641 aaaccattat gttttcgcct ggttttaaga gcgatagcat gggtgttagt ttcccttcaa
     5701 ccctttcagt ataaagtatc gatatcagct cgtcgttttc ggccgaggca atgctataat
     5761 accgtggttc tatttccttg gaaacaccca aggcaagcac ttggcctgca ataaaggggt
     5821 tatttttttt aaacttcaga atatatgcgc cgggggctac tgcggtattc gaaattacac
     5881 gctgtatatc gtaaggtatt tcgttccagc ttttcatcaa gataactttt gaacctaaac
     5941 atgctgaggg ctaaaaaggt tcgcctgttt tatccttatt ttacgttatt tccaaaatga
     6001 tacaggtagc ctacgtttac gctttgaaat acagcttttg actgctttgg cattcctgtg
     6061 taggtatgct tttgcatatc gccataacca tatccaactc gcccctctgc aaaaaaagtt
     6121 ccccacttaa aatcgcggct taagccaata ccaccaatca atccataatc gattcggtta
     6181 tcgcgtaatg gattaatggt tatgttgctc atctggtatt cacccgatga gttatcgccc
     6241 tcgcgcgcct gaaccaggta tgcaatatag ggtccggcat tgaaatgaat catgtaaagc
     6301 ttaaggttaa acctaacctg catgaagatg gggagttcaa tgtatgtgtt tacgcgtttg
     6361 taaagcgtat cgcccagctc tgccaaatca ataggcttac ggtaacccct gtttaccatg
     6421 taaagctcag actggaaacc aaagtatttc aggttaaagt acttaaaaac cagtccgtaa
     6481 tcgaatccat cggcaaaaag catacgggta ttctgaacgg gcgagaaagc aatatcgctc
     6541 atggtgtatc caccctttac accaacatag gtttgactat acgccgatag agcaaaaatg
     6601 gtaagtaata ctgtcagact tttttttatc atgtgctact ggttactttt ttaacttttt
     6661 tgttaccgat ttaactttcc catcaatccg gcctaaatca cttttcctga tatcgaggtt
     6721 gatgctcacg taaacccttt cacaatcgga ctcaagtaac ttataggctt tatcaaccac
     6781 atcgagggcc tgctgaaggg taggggtttc aactatggtt cccatggccg ttagcctgaa
     6841 ttcataaccc aacccgccta tcagctcaat aatcttagcc acgtaggggc taacacttgc
     6901 ccctttatcg gttgggaaca tggcaaaatt cattagtact gacattgctt attcctgttt
     6961 taatgagaac tgtaactcgc tatcccagtt tgccctgata tttataactc cctttgtttt
     7021 ttcatcggta aagtaaataa ccctttcggg ctgaattcgc ttcatgtagc tacccgaatc
     7081 ccatttcccg ttactgttag agtcatcaat aaaccgtaac ctatattttc ctggcttaac
     7141 aaagctgaat tctactattc cattcccctg aatggttctt gcatcaacag gtatgccctt
     7201 ttcgctcagc aattcaacca cagctgactt acttacgcca gttaggttga ttcttagggt
     7261 accatacttt tcagggtcgg caccaatcat ttccagcatt agggtatcgt tggtttgacc
     7321 tgtatagtct tcaaaagttc caggtaaaat ggtgagcttg tacgaggtat tgggtttcca
     7381 ttccttttca aatctgaaaa tccttgggtt caggctatct accttcaatt taatctgctc
     7441 gtcagctata ctgtcggtgg tattgaaaat cgatattttg gtgttatctg ccttccgaat
     7501 gggtatggga agtgtgagtt caaatggcac attcggtatt accatcctat cggagggcaa
     7561 agaggtgcta accgtaaaac ctttctttac ctggacggct tcatctttct tacctttact
     7621 cttggactgt tgaacctctt ccttgtagta aaggaatcgt aatgtatcgg tttgtggttt
     7681 aagcctgtat aacgagtcag ttttttggta ttcaagtatg gccataaggg tatcacgatt
     7741 ggctatctca tcatttacta tccaaagttt aagcgtatcg ccattgttat cgggttcaac
     7801 cacataccag ttctttaatt taagcctatc aacaggtttc aggctaaacc caccaacagg
     7861 ttttcgagaa aagcccaagg agagtatgtt gcgttcaggc ctatcgtacc ctgttactat
     7921 ttggttgggg agttcctccc tgaacagtct tatactgata cttttggggt ttttatcgga
     7981 tgggtttact tcataagaaa taaaaccaat actctccgaa ccctggttat ataggtaatt
     8041 gccgttttta tcgtcaacaa ccacaagctt gtactttatg ggctttaggt ttaccaatgt
     8101 gtattttccg tccttatcgg tttttgcaat atgattgggt agagtagtgt agggtagggt
     8161 atcggtaaat gaatcgtaaa gcattacaag ggcgttttca acgggctcgt tagtaattgc
     8221 attcaataca cgtcctgaaa gggtaagggt atcgattgtt tgcccggttg agaatgagaa
     8281 tataaaattt ttaatcgggt taccttcatt gttgtccctt actgcatcgg aaaagtaaaa
     8341 ggtatatgtg gtattgggtt ttagggtgtc ggtaagctca agctctagtt tcttgccttt
     8401 ctgcttgggt tggggtttac tttttagagg tggcgatacc aacagttttt gctgtaactc
     8461 cttgagctga ataaactcat caaattccag aacaattttt tgtccgctaa acccggttgc
     8521 gtttagtttt ggattactat tgactaaaac cgggggaata gtatcctttg gcccaccggt
     8581 tggcgaaacc accttggcac accgaggaaa cataagtaaa accaaaaccg aagcgccaac
     8641 tgtaattaaa atccttttca aggctatact attataggtt aaacttcgag caaagttaac
     8701 ctaatctatt gtatctttgc gctgattaac cttattttta tattgttatt gggtttaaat
     8761 caacctgtct gtttacttca aacttaaaac tgatgaatct tttatttatt attcttgggg
     8821 tagtaatcct tgtattaggt gctacatggc ttgttgaggg cgcatcgagt atagcaaagc
     8881 gtttgggtat atcagcatta accattggcc taacagtggt tgcttttggt acctcggctc
     8941 cggaattatc agttaacctt atttcggcta tagagggtaa ttccgatatt gcccttggca
     9001 atattttggg aagtaacatt ttcaatactt ttatgattct gggtttagct gccctggtta
     9061 aaccgattgg tgtacagcat agcaccgtaa aggttgaaat tcctttcttt ttactctcgg
     9121 ctttagtgct atgggtgctc tcaaacgagg ttttcctcga ttcagcagag ctaaatgtaa
     9181 tatcgcggtc ggcaggcttg gttttacttt tacttttttg tgttttcatg tactacaatt
     9241 tcctggttgg aaaggaggat ccagcctcag cctcgcccga aattgaaacc cgaaaacttt
     9301 ggatgtcggt gctgatggtt atttgggggt tagcatttct ggtaggaggc ggtaaactta
     9361 ttgtgcttgg ggctgttaat atggccacaa aacttggggt aagccagtca attataggtt
     9421 taaccattgt agctgcggga acatcgctac ctgagctggc cacctcggtt gtggctgcat
     9481 accgcaacaa acccgatatt gccattggca atgtggtagg ttcaaacatt ttcaatatct
     9541 tgctaatatt gggccttact gccggaataa accccattaa gttatacagt ggtgcaaacc
     9601 tcgatttagc cctaaacatt gctgctggaa ttctaatgtt agtctttgca gttagtggcc
     9661 caggtcgtaa gttcgaccga cgcgaggggg tgattcttgt gatggtttac atggcgtatc
     9721 ttgcttatca aattatcaaa gcctaactca ttatgaatca gcaagccaaa gagcatatac
     9781 tgcatttctg gacccgcaac ctggtggaaa agcctggtgc gttttcgttc aatctttttc
     9841 tctttttatc ttttggtatt ctatactcat tcaggatttt acaatcgcct tttattcttc
     9901 tggttttcgg aattattacc cctgttattc ttaccatttg cctttaccac atgtccgggg
     9961 ttagcctaca gcacttgctg ccaaaagttt ttcataaaaa aacaagcagg gttttcctgg
    10021 ccctgctcga ttgttctatt attacccttc taggtattct catttaccgc ggtatactca
    10081 actttttttt ctttaggttt ttacaaaccg ttatgcttcc aatactttac ctaataatgc
    10141 tccgggtttt actattatcg gaacacaact agttgtcgga gtactggtat ttgaaccgtt
    10201 taatgctctt tttgggagtt ttctcaaact cctggttgtg aaccacaagc ttggctattt
    10261 ggctatactg gggtagatgc ttgtttacct caaggcgatg gtgttcaaga accggctcca
    10321 gcttatcggg gtcaatacct tgctgctgaa ccgactcgta atcggggtaa atcaacccaa
    10381 taagtttacc cttttgctct atcacaagcg attcctgaac aaaaggaata ttattaagcc
    10441 gtgcctcaat ctcctcgggg taaatgtttt gaccacttgg gccaagtatc atgcttttac
    10501 ttcggccttt tatgtatata aaacctttac tgtcgtatac ccccaaatcg ccagtgtgta
    10561 gccagccatt gtccaacgcc ttttgggtgg cttcggggtt tttgtagtag cccatcatta
    10621 cgttctcacc ccttaccaga atttcgccaa cctcgctata tgggtccgat gaatctattt
    10681 tcatctcaag gtagtttatc accttgccgc acgagcccat gggattggta cgccaccccg
    10741 aatacgaaat aagcgggccg cattctgtca tcccataccc aactgtatac ttgaatttaa
    10801 tacgacgtag gaagcgttct acatcggggt ttagcgctgc accacctatt actatctcat
    10861 gaaaattgcc accaaatgca tttgatagtt tctcgttaat cttcttaaat attaccttat
    10921 ttagcaatgg aatgttgagt aaaaccttca tggcgggctt ttcaatctta ggctgtactt
    10981 gtttcttgaa aatcttttct atttccaggg gcacagtcag cactaagcgt gggcgaacct
    11041 tggcaaatgc ctcaagaatt atagggggcg acgggatttt ggttaaaaac gtaatatggc
    11101 accccatggc aaatgggaat agaaattcaa aagcacatcc aaatgcgtgt gccagcggta
    11161 aaaacgatac aatttgatcg ccctttacca ggtgcatgtt atttcgggca taatcggtat
    11221 tggctgccag actgttaagt ggcagcatta ctccctttga gaatccagtt gtccccgatg
    11281 tgtagctgat aaccccaagc tcactgtttg gtacttcggg atagttaacc ttgtcggggg
    11341 tgagactttt catctcatcg gtaattgagg attttgcagc ctcaaatagc cttcggcttt
    11401 cctcatcatt tgaaacaagg aggttaaacc ctgaaatgct gaatattgct tttaggttgg
    11461 gcatttcggc ttggttcagg ccgccccaaa ccatatcgcc tgcaaaaaga agcacagaat
    11521 cggaatggtt tacaatgttg tgtatctcgg ttggcgaaaa atccggtagt attggcacaa
    11581 taacagcccc atatgatatt acggaaagat acactatgcc ccactcagca ttattccttc
    11641 ccacaagggc aaccttatcg ccagttttta caccccatat ttgatatagt ttatggagat
    11701 agtgtatttt tcgggctaca tcggcatagg taaaggtttc ccctttaaaa tccgatagcg
    11761 agttgagttc ccagtgtttc ctaatgggtt tctcaacaaa ctcctttaca tagttgtact
    11821 taaccatata tgttgttttt accatgcatc cccgcaaata tcagcattca attgttgaaa
    11881 atcaacattt tgccattgtt ttatggttta ataaacccgc aaacgttttc ggaaagatgt
    11941 tttttttatt ttctctacat ttacttccct cacattctac cattccagct cattccgact
    12001 tttattcacg cttttacatt taacggttca catttaacgg ttacgtttaa atattcttga
    12061 ttcgtcttcc tctaacttct tctaactcca tcatattcac atgtttaact tttaacgatt
    12121 aacggttcac gattcatatt cctctaactt cttctaactt ctttatatta ctactcattc
    12181 cgacttttat tcacgctttt acttttaacg gttaactttt aacggttacg tttaacgatt
    12241 catgattcgt cttcctctaa cttcctctaa cttcttcata ttactactca ttctaacttt
    12301 attcacgcgc ttaacggctc acgtttaacg tttaacggac tataactccc tcatattcct
    12361 tccactccga ctcatcccga attaacccct taagtcattg cacttgggat aatttttgat
    12421 aactttcgga gccgataatc ttactttatg agcactaatc gttttgcggt tattgttgcc
    12481 ggtggctcgg gtcaacgaat gggttccgaa gcccccaagc agttcctgat cctgaacggc
    12541 aagcccattc taatgcatac cattgagctt tttttgtccc ttccccaaaa acccggtatt
    12601 gtgcttgttc tccccaaaaa ccagatggag cgatggaaaa acctttgcgt tgagtataat
    12661 tttaccgcaa atttaaccct tactgccggg ggtgaaactc gctttcagtc ggttaaaaat
    12721 ggtctttccc taatcccttt aaacaactcc cttgtcgcca ttcacgatgg cgttcggcca
    12781 cttgtttcaa aacaggttat tgaggagtgc tatgcaactg cagcccaaaa aggtattgca
    12841 attcccgtaa tcaaacctgt tgaatcggtg cgcctggttg agggcagtac ctctaaagca
    12901 tttgaccgta atagcgtttt acttgtgcaa acccctcagg tttttaaatc ggagctgatt
    12961 aaggaatgct ataacgttcc tgagcaaccc tactttaccg acgatgcctc agtagtagaa
    13021 tcgcagggat acaccataca taccgttgag ggtaaccggg aaaacattaa aattacaact
    13081 cctttcgatt tacagattgc tgaaatgctg atacgtagta aaccaattta actgtcatgg
    13141 ctaatttcaa acttgtagag gtaaacagta aacacacccg taacgatttt cttgaactac
    13201 cgctttcgct ttacagcgat tatccaaact ggatacgccc gcttgacatg gatattgaga
    13261 atgttttcaa ccccgaggtt aacaagcatt tccgccatgg cgaggctatc cgctgggttt
    13321 tatacgataa cggtaaaccg gttggccgaa ttgctgcttt ctacgataaa gagattgcca
    13381 aaaactctga gcagcctacg ggtggcatag gattttttga gtgcatcaat agccaggagg
    13441 cggctaacct gatgttcgat actgcccggg agtggctcaa atcaaaaggc atggaggcca
    13501 tggatggccc cattaacttt ggcgaccgcg atcgctggtg gggcctgctg gttgatggcg
    13561 atttcccgcc caactactgc atggattacc acttgccata ctaccgcaac ctcttcgaaa
    13621 actatggatt taaggtttac ttccaccagt acacctacca ccggttagtt aactctgaaa
    13681 atgttgaccc ggttgtttgg gaaaaggccg agcgtattgc tcgtaaccct tcgtacacca
    13741 ttacaacaat atcgaaaaag aatttgaaga agtttgccga ggatttccgc accatttata
    13801 acaacgcttg gggccgatac accggtgtta aaaaaattac tgaggctcac gccatggctc
    13861 taatgaaaac catgaagcct attattgacc ccgagctcat gtactttgct tactacgagg
    13921 gcgaacccat tggatttttt atcatggtac ccgaccttaa tcaggttgta aggcacctga
    13981 acggtaagtt cgatatcctt tccaagttgt actttttgta cttacttaaa gttaaaaagg
    14041 tttgcaccaa ggccattggc ctgatttttg gtattgttcc aaagcaccag ggaaagggag
    14101 ttgagggtgc gctggtaaat gaattcgcta agcgggcttt agcccctgga ttccgttaca
    14161 ccgagctgga gctgaactgg attggcgatt ttaaccccac catgcgacgc gtggccgaac
    14221 aaattggtgc tcgggtgcgt aaaacacaca taacctaccg ctacatgttc gaccccacca
    14281 aggaggttgt ccctccccgt agggtcagct aggtaggttt ggtgatggtt gttagtagat
    14341 agtaaaagtt taaatttgtt caacaagaaa gtagaaaaaa tggaaactaa agaaaatttt
    14401 aggaaatacc ttgatgagta catggttgat tttttcccag aaataaagga aaatatggga
    14461 atagagtacg aagacttttt gagggcaaaa acggaaatgg catttagatt atataatgat
    14521 atgattgaaa ggggagaaat gccattaatg gcagaactcg gagcaataga agttttaaaa
    14581 gagggatata ctaattcaaa atactcatac attgaggaaa ggataagaga aatggatgaa
    14641 gattattatg aaagattgga aagtgaagga acgttaaaga aaaagataat tgaaatagtg
    14701 aaagcaggag agggaatatt tgagaagtac gggtttgaaa agattgattt tgcatatacc
    14761 gatgagtttg aagatgaaat gatggaagta attaaattaa aattatagtg tatgagttac
    14821 aataaaagac aagtgtttga ggacaacctt gaagcaataa gaacttacta tgatattaca
    14881 ggtaatgaaa gagaaaaaac gaaagaagat attgaaaaaa ttagtaaata caaaggattt
    14941 ggaggtttaa aggttgtgct atgaatccaa atgaaattac atgttggtac attgagtatc
    15001 ttcaactaaa accaaaagtg gaagaactaa atgagttagt aaaaaaaata tagagagaaa
    15061 gaattgattt tttaaatata tcttttaacc ctttatcctt taacctttat cctttatcct
    15121 tataaccttt atccttataa cctttatcct tttcaatttg aaatcttaag tatgaacacc
    15181 aaatctatcg cttttatcgg tttgctttgg ctatcggcat tacttgcctt tggccagcgc
    15241 gatattgaac caaggctgct tttctccgat acggcaagct tcaacttcac aggcgagtgg
    15301 cagtacctca caaccgacat ctttctgcta aatggcgata agttcagtac gctcatcaat
    15361 gagctcgatt ttgcaagggt tgaacccaag aaaaaaaggc gcaaaaagct gggtgtggag
    15421 gaggagcaac ttgagtacct ctttattacc gctagcctta agaacgtaaa gttttttggc
    15481 gataacgata taacctaccc cctctataac ttccagattt cacgcgacaa ggatagcaag
    15541 taccaaacct ttgtaagcga taatattgat tatgtccgca ttatcgataa cctgccctta
    15601 tactcggccc gcgattacat cgatgcggaa atacgtgttc gtgccatcac caataacgac
    15661 cgcgaccaaa tgcttgcgct tgttgcctcg cagctgaaaa acctttcaaa gattaccact
    15721 ccaaccgatg ccgttatgag cattataggc gagtttggca attttattga ggctaacacc
    15781 aagaaaaagg agtaccggtt tagctctacc atcaggttgt ttgagcagaa gaatttcgat
    15841 acccgcctac actccataag gctttacttg ctttctactg caaacacgcc tgcacttgag
    15901 ttttctggtg taccacttcg tcaattcctc gataccgtta caaatgggcg ggttaaccgt
    15961 aaccagctgc gggaacttat tggcatccgg agctaccctg taattgtagt ggcaaactac
    16021 aaatcgttat accgcaccga acaaatttcg ggcgacgagg taacctttgc caatatcgaa
    16081 aagcgtaaac ttaaggttga gaacgacttc cgccagggac ttatcaatgc cgaaacttac
    16141 cgccaggaaa aggatttact caatttcctc aacatttttg cccagctaaa aaatcacctc
    16201 gacatttaca gcctaaacta tcgaaccggg aataacgatg ccatttcggt cagtcttttc
    16261 cgggttatgc agtactaccg tcagctacaa aaagcctatg aggagatgaa gtataagtat
    16321 cggggcaata atactttttc aaccgttttc aaccaggagt acgaatcaat cctcggcttt
    16381 gcctcgcttt acatggataa cgaccacaac ctaaaatcga ccaagaacct tgttaacacc
    16441 cttgttcgcc tcgaggccga tcccaaagta cccaatgccg acctggaaaa atacattgcc
    16501 gacctaaggt tctccgatat ctttaagccg gagctgatga gccaaaacct cgagggtcaa
    16561 atcatcgcga accacctctc aaggctggag gagcaacttt tcaagggaac ctttgaggcc
    16621 gaaattgaaa gggttcggaa agccgaggca aatcccaaaa acaaaaccat taccgataac
    16681 ctactccggc ttgccaagtc aacctcttgc tcgctttgcc gcgagcgttc ccttggcgca
    16741 gtaacggagt ttacccaaag gctcgatgca tactacctta aagtagaact tcagcgctac
    16801 gacagcattg caacggctct tcaaccatgg gtttttaatc gcattgagct cattcagctc
    16861 atgcagcaaa actttaagca tttgtacccc gataacgcaa accttgaaag cgcacgtttc
    16921 ctgaacggga aaattactga gattgaacgc gatgtgttca acatacgtga ttttctaaaa
    16981 atcgatttaa gcggtaaaga cctgtccatg gtgaagaact tcaacgagaa actgcttgcc
    17041 attcagcgcc aggccgatgc aaacatcgat ttgatttgca agctgcgtcc ggaactctgc
    17101 tccaggcagc aagcagccac ccaaaacatg aaaaacactg agctatcgaa tctttttacc
    17161 cgctccgatt ccgtagcgcg ccaaaccgag gttttttact ccatattcca gttccagctc
    17221 aataggttag cacgggtacc tgaaacatcc aaaaacgacg agctacggat tgaaaccgaa
    17281 aggctggccc accagctcga tgagctaaag gtagccatca gcctgctcga tggcaaaaac
    17341 ataacccagg aaacctactc caaacttgta aagcaggtaa accaacaggt taaggatatt
    17401 agcgacaagc ttgtaaccct taacgagaag gtaagcctta gcagtaaccc ctagcctaag
    17461 cgcaataaat cagccagaag aaccaagttg ccccagaact gtttttagtg tttggttttt
    17521 agtgtttagt attttcacta tcaactgtca actgtcaact acatttgtca tcccgagcgt
    17581 agtcgaggga tctattgaat agtgtgcagt gtacagtgtg cagtttacag ggaagatgga
    17641 aagtagtgtt tggtttttag tgtttcgtga aatgcttcta tgcactatca cccatcaacc
    17701 atcaactgcc aactccgctt gtcattctga gcgcagcgaa ggaactctaa tcgcccctaa
    17761 agtgattttt cgctaacgct caacatgaca ttaccctcac taacaaccat caactgtcaa
    17821 ccatcaacta aatatgtttc atagggttac acggtgtaac acggtgttac acagaattgc
    17881 atttaccaac aacgaacaac gattcacgag caacgattcc agccccgtag gggcgtaata
    17941 tttgtaactc caagcctacc cccatatgta ttgcgatgca cgcaatgcat atgccaaaaa
    18001 gatacactga agctttgcat cgcaacagaa gagcatgtca aagaacgata aaaggtatta
    18061 agggaataaa ggtacaatgt gatgcttcgg ctagctcagc atgacaaagt gagtgaggga
    18121 gccaattgtg ttgtcattcc gagctagtcg aggaatccca ttaaagggga tgaaagggaa
    18181 aagcgatgct tctacagctc tcagcatggc aatcacaaca taaaccaagc aacagcaagt
    18241 atcacagcgt ttcacaatgt aacacgctgt taaacaaaga tattcacgaa caacgattca
    18301 cgaacaacga acaacgcttc tagccccgta ggggcgtaat atttgtaact ccaagcctac
    18361 ccccatatgt attgcgatgc acgcaatgca tatgccaaaa agatacactg aagctttgca
    18421 tcgcaacaga agagcatgtc aaagaacgat aaaaggtatt aagggaataa aggtacaatg
    18481 tgatgcttcg gctagctcag catgacaaag tgagtgaggg agccaattgt gttgtcattc
    18541 cgagctagtc gaggaatccc attaaagggg atgaaaggga aaagcgatgc ttctacagct
    18601 ctcagcatgg caatcacaac ataaaccaag caacagcaag tatcacagcg tttcacaatg
    18661 taacaccctg ttaaacaaag atattcacga acaacgattc acgaacaacg agcaacgctt
    18721 ctagccccgt aggggtgaaa catttgtaac tccaagccta ccctcttgtt agcgcgcctc
    18781 tgttgacgcg tgcgggcatg tttgggggag agcacaagct aaatgctacg ctacagccag
    18841 acataaaaag gtttttttgg acgtgcgtaa actaggggat tttttttcta cattcaatga
    18901 aggctcccag gcggagtagc gtatgccgta acaaaaactg aagaaaacag ctgaaaacgt
    18961 ttcgctcttg tcaaaaaaat gacaagtgct aactttacaa aactatttag ctagcacttt
    19021 gtctttggca cccacggctg ttgtgccgca tgtaagaaat tgtttacact tattgatttg
    19081 aatcttgtga ttagtatatt tgcaatatgt tgaataggga tgatatacta aataagttaa
    19141 gagagttgaa accattcttg aagagggaat actctgtaaa agaaattggt ttattcggtt
    19201 cctttgcatc tgacagcgct agagatgata gcgatattga tttacttatt gaactagaaa
    19261 aacccatagg ctggaaattc ttctcattag aaatctactt agaacaaatt tttggccgaa
    19321 agatagattt agtcaccaaa aatgctcttc gcgatagatt aaaagaggat attcttaaca
    19381 aagtaaatta cgtatagtgt ggacaaggac aaacgcacat acttaatgta tcttgaagat
    19441 atccagctat caatttctag aatttcagaa tatattgcag gtatggattt tatctctttt
    19501 aagcgagatt ataaaaccat tgacgcagta attagaaact ttgaaataat tggagaagca
    19561 tctaaaaatc ttcccgattt cgttaaagaa aaatacccaa aaataccttg gcgagagatg
    19621 tattatttga ggaataaggt atcacatgaa tatttcggaa ttgattatga aattatttgg
    19681 gatattgcga ccaattacct tcctgaaaac aaattactca ttgagcaaat aattttggag
    19741 gaatccagaa aataaaactt aatgtacaac aacttgtttt atttgggtga attccacttg
    19801 catgattatt ctcttttaca aactatcccc ttgttagcac tcgtctgtgg acgcgtgcaa
    19861 gcaaagtagg gggtgagcat aagctaaact ctacatatca gccagacata aaaaggtttt
    19921 tggggctgtg cataaacaag gggaattttt tctacattca atgaaggctc ccaggcggag
    19981 tagtgtatgc cgcaacaact actgaggtaa actgctaaaa aacgtttcgc acttgtctta
    20041 ttttgacaag tgcgaacttt acaaaactat tcagctagca ctatggtttt gccacccttc
    20101 ctataaacag aatggtactg gttggtttat gccttaaaat aaacatgaat ggcctgttgg
    20161 catcaaaaac gttgaagttg ggcatcgatt tcatggccac cactacggca gtagccgctg
    20221 ctgcggttgt tccttcttcc gatacctcaa taaaggcttt atggtaagca tcgcttatct
    20281 ttaaatcctt attccccgat attccggaaa aatcggcatc atcgctaaag ggtatggtta
    20341 aacccatctc gccaagtact ttcttcattg agaagccgct ctcaacggtg aaccgtggta
    20401 taaacaccag ggttttttgg ggttctattg cctttaccac ctcgttatac ttttcaacgg
    20461 taaatgcttt ctcaaattca ctcagcggtt ttccctcgtt gggcagtatt agcagcatcg
    20521 atagctcttc gcccttatag tccatttcca gggcagcaaa gtcggttcct ttgaggtaac
    20581 caaaggtttt gtcggcgctc ataaactgat gcttggtttt tgagccatcg gccagggtaa
    20641 agtcgcgctc cttggttttt tcgggttcaa aggtttcctt ccactttccc ttaaagtaaa
    20701 ccgcattggt aagcacaagt cgggttaaat tgctaatact accctgtggt agcaaatcct
    20761 tgatgtggcg ttgcgtatcg tactccacgg catggttgat agttttacgg gcagcctcgg
    20821 ggtcattaat aaaatcaacc ttatacaggc ttgttttata gttgcccttc aggtcgcacc
    20881 tatacctttt aagtatcttg taatcggttt gcagccatgc cttattggca acatttatag
    20941 taatgtcagc cgacgacgac ttaattaaac tttgctgaag cttaccaact gcctgatgaa
    21001 actttgggct attgccaaaa tgaaaggtag cgttcagctg gttaagtgtt tcgcccctgg
    21061 ctccaagcgt aaccattcca aatgcgggtg taatgctgaa cggtgagtaa accaggtttt
    21121 cgttacctcc aacctttagg cccctgtaca ggtcaaacgc aaaagtattt attgattctg
    21181 atgcactttg tgcatgggtt acagtgcctg ctagcactgc aattatgaac gacgaaaact
    21241 ttttcatatc gatagtttta gggttaacca tggctacagc gtaagattca aatatcatgc
    21301 taagttcata attttaaaca ttttttacaa ttttcttttc ctgcaatttt actatgtttg
    21361 tattcaataa ggtatggctt ggttaacggc tctcttttaa cctaattgcg tcctgagtaa
    21421 tgcgaaggat attgctttta ttaccatgaa catgatattc gctgcgctca acatggccaa
    21481 aaaccttatg gaaacaacat tgagtaaaaa tcaacattgc tgatgtcaat tacagtaaac
    21541 agtatcacca agcagtttgg caagcaggtg gctttaaaca gcgttacctt tcaggttcca
    21601 caggggcagg ttgttggcct tttggggcca aatggtgcgg gcaaatctac cttaatgaag
    21661 attatcaccg gctaccttac acccgatagc ggctctgcac ttgttaacgg tgttgacatc
    21721 aactcgcctg cctacgattt ccgccgcgat attggttacc tgccagaaca caacccgctc
    21781 tacaccgata tgtacgttaa ggaatacctt ttgtgggttg ccggaattta ccgtttgggt
    21841 aaaaatgccc gtaaacgtgt tttggagatt attgatatta ccggtatagg acacgagcaa
    21901 aataaaaaga ttggcagcct ctcaaagggt taccgtcaga gggtgggctt ggcccaagcc
    21961 cttattcaca acccatcggt tttaatcctc gatgagccca ccagcggcct tgaccccaat
    22021 caaattgttg aaattcgaaa tctgattgcc gcggtgggtc aaaataaaac cgtaatcctt
    22081 tcaacccata tcatgcagga ggtggaggca atatgcgggc gaataatcat tatcaacaag
    22141 ggtagtatcg ttgccgatgg acctgcttcg gagataaaag cgcttacctc atcgtcggtc
    22201 caaaacgttg ttgtggaatt cctggaagcg ccatccgttg aacttcttca gagtatcgag
    22261 aatgtttcaa acgtaaatca actaactgca accacttttc tggtttcatc gtcgtcaaat
    22321 accgatatta ggccaacaat atttaacttt gccaagaaca acaatcttac cctgttaaca
    22381 cttcagcaaa aagtgcttaa cttggaggac gtttttcaaa accttacact aaccgagcca
    22441 aataaccgtt aacaatagta acccaaacgt aaaaaacaag atatggaaag cacaaaatca
    22501 tcaaaagcag tatcgttcct atcggtactt attgtcattc tgctagttgt aattgccggc
    22561 ctttcgtacg tttactacca gcaaaaaaag gaggcaaagg aaaccgaaac catgttgctt
    22621 gccgaaaagg attcgctcag caacaacttg cttaacctga tgaacgacta caaggatttg
    22681 gaaaccgaca acgattcatt gaaccgcaag cttcaaatgg agcaggagag ggcacaaaag
    22741 ctttactcgg agcttcaaaa cctcaagcgc gttagctacg ctaaaatcaa ggagtaccaa
    22801 aaggagctcg gaactcttag ggctatcatg aagaacatgg ttcgcgaaat agactcattg
    22861 aataccctta accagcaact tattgctgag aatattaagg tgaaggaaga ggcagccacg
    22921 gctaaaaaaa ccgttaagga gcttgagcag cgcaccgagg agctaaatac ccaggtggca
    22981 aagggttccg tggttaaagc acgcaatgta acagctgttg ctctcaatag aaggggaagc
    23041 gaaacatccc gcgctagccg tacggctaag atcaaaacct gctttactct taatgagaat
    23101 gctattgcca aaccaggctc acgctatgtt tacatgcgcg tagtaggccc cgacgatttt
    23161 gtccttgcaa aatcggaaac cgaccttttc gattttgagg gcgaaaagat tgtattctcg
    23221 gctaaccgtg aggttgatta ccagaataag gatgttgaca tgtgtatttt cttcgacagt
    23281 agcggcgagc tggctaaggg cacttacaaa atcacccttt acatggatgg ctacatgatt
    23341 ggttataccg aattggttct aaagtaatgg catagcgtcc aataaatact aaaagcgccc
    23401 tttggggcgc ttttttcgtt tacctatttc taaatatatc cctttagggt cttattgata
    23461 taaggataaa ccagaacagc aactgcagca ccaagaatat cggcaaaaaa atcggcccac
    23521 tcagcaccgc gcgacgtaaa aaggtattgc tgtgtcaact ctatcaaacc accgtaaacc
    23581 gaggctacaa taaaacccac ccatttatgc cgatttttaa ccgtgagctg aaccctctgg
    23641 tagttcattt ctgagataag taaaagggca aatacggcaa ataatccaaa atgaaccatt
    23701 ttatcaatgc cgggtatttt aatggtgggc aaaccgcttg gtggcattcc gcataggata
    23761 agaattacca gcgcccacat caatgaattc ttatacttta caatgagttg ataggttttt
    23821 gctaacattt tgctctactg gtttacttgc tcaaaggtat aaccaaaaac ttcaatttcg
    23881 tgtttaaaga gtttttcaac tctttccttt tgttgtttgt taaagtactg gctatagtgt
    23941 tttttgcctt ttcggggttt gctttttagg cgtggcaagt ctcccagttg tatccctagc
    24001 attttttcga ggtcatttat gcctttgtta agattttcga acttaaggta aacatcgcaa
    24061 attggcttcc cttcctcatc aaagtaaaac cttgagttgg tccaggtttt atcaaagtat
    24121 cgtatgaaat tgctaaacga tgatagcaca aaaatcaatt ttatcctgtg tagtaacctt
    24181 ttatacctta ctatggcaaa gggttctctc aaaatgttag caacattttt cccgatactg
    24241 ggccataggc gagccttttc ccagtgataa cgtgaaacaa ccatatccca aggatttcgg
    24301 acaatggtaa tcttaaggta ttggttccaa acctcttcgc caaccttttt tttaattgca
    24361 atcgccgaca tatggttctg aaatccctcg caattcctgg gttggtattc atagtaatcg
    24421 gcgtcgaatc gctcatcaaa aggtgccagg ggcgttaaaa catcatcggg cccacatact
    24481 ttgcttagca ttagctcaac gctagtactg gcagttttgg ccgatttaat gaaaataaac
    24541 ctatgcctat gcgaaactat catttatctt actcttctta tcaacaggca gttatgattg
    24601 ttggttaaac tacaatttcc gaatatactg ttttttgctt ttactttatg tagatatgca
    24661 atatacgcca aaccctttaa cactatattg attttttctc ttgttaaata gggttttggt
    24721 gcttacattt acagcaacca taaaaaatca acagctatgt tcgaaaagca agtttacgta
    24781 gaacgcagat taaaactccg gaaaaagatt aaatcgggtc tggtgattat ccttgggaac
    24841 accgaaagcc caatgaatta taagtcaaac acctaccgtt tccgtcagga tagtaatttt
    24901 ctctatttct ttggtctcga cattccgggg ttagtgggtg ttattgatat tgaagacgga
    24961 aaagattgtt tatacggcaa cgattttgac atcgatgata ttatttggat gggcccccag
    25021 ccaaccctga aggagctggg cgaaaaggtt ggtatccttt caactcaccc tttcaaggag
    25081 ctttactcta ctttaagccg cgccattaaa cacggacgcc gaatccattt ccttccccct
    25141 taccgtgccg ataatgtttt actgcttgaa cagctgctag gaattaacca atcggtcatt
    25201 aaaaactatt catccgttga gctgattaaa gccattgtaa gccttcgttc agttaaggaa
    25261 gatgttgaaa ttgccgagat tgaaaaagca tgcgaggtag gttaccagat gcacgtaacc
    25321 gccatgcgta tggccaagcc cggaatcagc gaacaggcaa ttactggagc tattgagggt
    25381 gttgcaattg ctcaaggata catgtcttcg tttccaatca ttctgtcaca gaatggcgaa
    25441 acccttcata accacgacca ttcccaaatg cttacttcgg gtcgcctgtt attagtcgat
    25501 agcggagcag aaattagcag ccattacgct tccgatttta ccagaaccat accggttgat
    25561 ggtaaattca ccgaaaaaca gaaagagatt tacaacattg ttcttgctgc aaataacaag
    25621 gccatcgaaa tttccaagcc aggaatcccc tatatggatg ttcataaggc tgcagcgcgt
    25681 gtaattgccg aagggcttac taaactcggc ttaatgaagg gtaatcccga tgatgcagtt
    25741 gccaatggcg cacacgccct cttctttcct catggccttg ggcacatgat gggcctcgat
    25801 gttcacgata tggaggatct tggcgaaaac tacgttggct acgacgatga aatcacccgc
    25861 gccgaccagt ttggtatagc ctacctaagg ttgggccgtc gcttgcaaaa aggatttgtt
    25921 ttaacggttg agcccggtat ctactttatt cctgccttaa tccaaaaatg gaagtcggaa
    25981 aagattaatg aatcatttat aaactatgcc aaggttgagg aatatattgg ttttggtggc
    26041 attagaatag aggacgatat tcttattacc gaaaatggct cccggattct tggaaaacgc
    26101 attccggtta cagttgaaga aattgaagca ttgatgaagc attgatactg acctttgtaa
    26161 ccactagccc atcggaccaa acccgatggg tttttttcac taaaatgctt cagttaccgt
    26221 aaagtagtaa gccatttctt tcctatcgct ataggccgca tcaaacctga ggtttacctt
    26281 tatggggttt accctaactc ttactccagc tccataagtt aacttgcttt tgcttatatc
    26341 taacttgtca aagtaaggtg caacatcgcc taagccacca aaaatagtcc ctttaagcca
    26401 tttatatatc ggaaatctat attcaccctg taggcaagcc attgcgttgt cgcggtatcg
    26461 gccctggtag taacctcgca taacatcagc tcccccaatc attggcatta gctgaaaagg
    26521 aactttcccc caggtcatat cggctaggaa ttgaagggca aatacatgat cgtcgtaaat
    26581 attatagtag tttcttaaat cgatggttag ctttgtaaag ttcaactcgc tgccaaatat
    26641 cttactgtaa accattagcg aggctttata gaactcacca aacgatgggt agtaaagatt
    26701 ctccctgttt tcccaggtta aaaggacacc caagcccgtt gttaaccttt cgtttactcc
    26761 agatatgccg gttgccagca agcggtcgcc ggggtagtct ccagttctgt agtaattaag
    26821 ttgaccttga acaccagcca ttattacacc aaaaagtatt ctttgatgtt ggataagaac
    26881 cgagtaatca tcggaaacat acttttcctc cagggtgtcg ggtgtgttcc ttccaactcc
    26941 ccaaaatttg tttgggaaat ggtttaccct tatatgaccc gaatagtagt gttctctatc
    27001 agtggtatat attctaggcg atatccataa acttgcctga ttcttaaggg tataaacggc
    27061 agtcccctga acgctcgata ccttattatc cctacatcta aagtagtatc cacccgataa
    27121 tccaccgccc cagtttgtct cttctccata aaatgcaacg ggtactacta caaagtaatt
    27181 attatcatta cttttcttgt ctacgcatag cgtatccgtg ttgctacccc aaccagaatg
    27241 tccgcaaata aggccaatag ccataaatac aaacataccc aggcaatttg atttcattac
    27301 aaaccttttt gattccactt caaatataaa caaaaccaac catgcatatt tagcttgatg
    27361 tgagtatttg gttcaatacc tgttaatatt tttttaaact taacccttga cttgtttcac
    27421 taaacctaca tataatattt ttttccaacc aaccataggt tactataaaa ttgtttaacc
    27481 aaattattaa ggtattttca acttcaacta acagattccc ttcccctaat tttattaaca
    27541 tatgaccttg ataacaactg ttaataagta caaatttatc ctgtattaca atgacttata
    27601 aattttatga tgttaatagt ttgttttaat ccatttaata accaattttg caacaccctt
    27661 ttcaataaaa ttatgaaccg agctaacagc ttaatatcat catccttttt ttatctttta
    27721 aatttttaaa aaaagaatta atgataaata aagaatctgt tgataagttg ggatatatcg
    27781 atgaacctat cgattcgggt atcaatcttg taaatgaaat caaccgttta aaggttgaga
    27841 aaaatgctgt gattcttgcg cattactacc aaagccccga aattcaggat attgccgatt
    27901 ttgtaggtga cagtcttgct ttagctgtaa aggccactac attgaagtcg aaactaataa
    27961 tatttgctgg agtcaaattt atgggtgaaa ccaccaaaat tttgttacct gatgcaaaaa
    28021 ttgttttacc cgatatgaat gcaggctgca gccttgccga ttcatgtaat tataatgatt
    28081 tcaagtcatt caaagaaaaa tatcctgaac acatagttat aacctatgta aatacgaatg
    28141 ccgatattaa aactctttcc gatatatgtt gtacttcttc aaatgccgag aaagtaattg
    28201 aatctattcc aatagaaaaa cctataatat tcgcacccga cagaaatcta ggtaactact
    28261 taaaaaataa gttgaagcgt gaaaatatgc ttatttggga tggtttttgt catgttcacc
    28321 agaggttttc attggagaga ctaattgagc taaagaaaaa gaacccaaat gctaaaataa
    28381 ttgcccatcc ggaatgccag aaaactgttt taataatggc cgattatatt ggttcaactt
    28441 cacaacttat tgattttact attaatgatc cttcaacttc atatctagtt gcaactgagt
    28501 atggtgtaat acaccaaatg aagaagtttt cacccgaaaa agattttatt cctgtacctc
    28561 ctgacaattc tacatgtgca tgtaacgatt gtatttatat gaagatgata accttaaaaa
    28621 agctatattt aagtctaaaa tatgagtatc cggttgttga tttaagtcct gaaattatta
    28681 caaaagcaaa gttacctatt gaaagaatga ttgctataag gtaatggaca gtaaaattat
    28741 aatagataaa gaatttgaaa agcatattcg accagttgag tttaatcaat tcaacggaca
    28801 gaatgatatt gtaaagaact taaaaatatt cgtaaaagct gctaagttaa gaaatgaagc
    28861 actagatcat gttttattgc atggtcctcc cggattagga aaaactactt tagctaacat
    28921 aattgccaat gagttaggcg tttcaattaa aactacttcc ggaccagtac tcgaaaaacc
    28981 tggcgattta gcaggtttac ttacaaacct tgaaccacac gatattcttt tcattgatga
    29041 aatacatagg ttaagtccag ttgttgaaga atatctgtat tcagcaatgg aagattacaa
    29101 gattgatata attattgata aaggtccaag cgcacggtca atacaaatag aattaaatcc
    29161 attcacttta atcggagcaa ctacccgcag tggattactt acaagtcctc taagggctcg
    29221 ttttggaata aaatttcttc ttgagtacta tactgttgat attttagcag gaatagtaaa
    29281 acgatcagca tcaattctaa aaattgatat tagagatgat gcagccgttg aaatagcaat
    29341 gcggagtagg ggaacaccaa gaattgcaaa tgcactatta cgcagagtac gtgattttgc
    29401 tcaggtaatt ggtaatggtt atatagatat tgatatagca aaggaagctt taaatgcttt
    29461 aaaaatcgat tccagagggc tcgatgagat ggacaataaa atattactaa caattattga
    29521 aaagtttaag ggtggtccgg ttggtttaaa tacaattgca actgcagttg gtgaagatgc
    29581 tggtaccatt gaagaggttt atgaaccatt cctgatacag gaaggatttt taaaaagaac
    29641 accacgtggc cgcgaagtaa ccgatttagc ctataagcat ttaaataaat taagatttcc
    29701 tgagcaaggt aagctcttct aaaaaatgga atccgatatc agattcatct ttttcttatc
    29761 gtttatctta atttttttcc ctttgattgc tatcaaaccc tcgttcttaa attcgcccaa
    29821 aatacgaatt acagattcgg ttgctgtacc aacaatactc gataaatctt ctctagttag
    29881 atttactgct atatagccct ctgcgtctag tttgaaagta ttttccagca ttagtaagat
    29941 ttcggcaagg cgctgtttaa cagttttttg agctatatct tttataaata tatttgcctg
    30001 atccaattcc ctacatgtta gctgtattaa tgaaagagca aaatcgctat tttttttaat
    30061 taagttaaag ataatttccg atggaataaa gcaaatttct gcttcttcaa taatttcagc
    30121 tgttgtacaa gccatttcgt tgctcaaaac cgatctgtaa cctgttatat ccccctttat
    30181 tgcaaatgca acaatttgtg gcttagaatc aattcctgtt ttgtaaattt ttaaaatacc
    30241 ttttttaacg cagtaaatgc ccgtgatcct gcttccctct ctataaatta tttcaccttt
    30301 tcggtaggtg ttacatttta agtttgcatt caatagttca agttcctcgc tattaagcga
    30361 tttaaaaatt ggaaaacttg aacttataca agtatttgaa tttccgtctt tcagcatttt
    30421 cagtaattct tgaactgttt tagaaaacga acatcattct caaagtaaag acgcaaatcg
    30481 cgaacctgat atttaagcat tgctatacgt tcaagcccca taccaaatgc gaaacctgaa
    30541 taaattttac tatcaatacc gttaagttct agtacatttg ggtcaaccat gccacatcct
    30601 aaaacctcta accagccagt atatttacaa accgaacaac cttttccacc acaaagctta
    30661 cagctaacat ccatttcagc cgaaggttct gtaaaaggaa agtatgaagg tctaagcctg
    30721 atttttgttt ccgaaccaaa catctctttt gcaaagtaaa gaagtgtttg cttcaaatca
    30781 gcaaatgaaa catctttatc aatgtaaagt ccttcaattt gatgaaaaat acagtgagct
    30841 cttgctgaaa ttgcttcatt tctgaatact ctgcctggac aaattaccct gattggtaac
    30901 ttctcatttt ccatgcttct aacctgaacc gatgatgtat gtgttcgcag aaggatatct
    30961 ggcttttttt caataaagaa agtatcctgc atgtccctgg caggatgttc ctcgggaaag
    31021 tttaaggccg aaaatacgtg ccaatcatcc tcaatttcag gaccctcata aacagtaaaa
    31081 cccaaatatt taaaaatttc aactagttca ttcttaacta gtgaaattgg atgtcgggtt
    31141 ccaaattcaa atggatactc ctctagcgaa agatctaaat tagttccttt tgattttgta
    31201 gattcggaaa atgttttttt ccaggtttca accttatcgg atatttcaga tttgagctgg
    31261 tttagtattt taccaacttc cttcttcttt tcgtttggaa tatttttaaa gtcatcaaat
    31321 agttttgtca gataaccatt ccttccaaga tactcaactc ttattttttc aacactttgt
    31381 aggtctttag gatctaatgt cgaaaacgtt tcccttatct gtttaattct ttcctccatt
    31441 ttaccaacta ttttacaatg cgaactttga acttaatatc ttttaaaggt ctatcgttgg
    31501 aatcggtttg aacctgcgaa attttttcaa ccacatcgta tccttccaca atttcaccaa
    31561 aaactgtata gtttccatct aaatgtggta tgcctcctac agtagtataa attttacgct
    31621 gattttcatt aaacttgata tgtttaacct tcataatcga gtcagctaag atagaaacct
    31681 tttgtgttac ttcattaaga aattttgggt ttaagttgct ttggctacct aacagactat
    31741 cgtaaagctt cttttctgtt ttctccctta attttttgtt tagcttgtct tcaagcttat
    31801 taagttcttc ttcagtaaat acctttccaa caacaaggta gaactgtgat gatgaagatt
    31861 ttttttctgg gttcacatca tcgccttccc ttgccatcgc tattactcca cgcttatggt
    31921 aaattgtatc aacaatttct gcatcaatta aataaccggc atccttttcg ccatatagtt
    31981 ttcctggctc agcattctta gtttcaggat caccgccttg aacaacaaag tcttttatta
    32041 ctctgtggaa aataagacca tcgaaaaaat tctcttcggt caatttgata aaattttctg
    32101 tatgcttagg cgtttcatta tatagtttaa taactatatc tcctacatca gttgaaattt
    32161 taacatatct attgttatcg ttgtaattcg tagtgcacga tagcattgat accagaccga
    32221 gtaaaactaa agttctaatt ttcattgctt taacttttat ctttcatagt tacttgtgca
    32281 aaaataccaa aaattatact tttgacattc atacttggaa aaatgtcatc cgtaaaacaa
    32341 ctttattcac agtcaattat atatggatta agtaccattg tccctcgttt actaaactac
    32401 ttgcttgttc ctatacacac taacgtttta acagacccta ggcagtacgg tgtaattact
    32461 gagttttacg cttacattag ttttttacta atattcttga cctttggttt ggaaactggc
    32521 tatttccgtt ttgtaaataa gtataaaaat gatgagcggg tttattctaa tgcatttact
    32581 tttgttctaa cactttcatc catttttttc attgctgctt accttttttc agattcaatt
    32641 tcaagtttat taggatctgg gtatttaccc atttacatta tcactactgc agcaatcgta
    32701 tcgctcgatt ccatcaccgc tttacctttt gccaaactta gaaaccagaa tagacccctt
    32761 gtattttcgt ttattaaaat taccggtgtt gccataaatg tattgttaaa tattattttt
    32821 tacctggttc ttaaaccctc atacttaaaa aatttattcc cacatatcga ccttttgttt
    32881 tatgtattcc tatctaactt tatacagaac attgttgttt ttgtattggt tacctatttt
    32941 actggtttcc caaaaattag aatcgataaa agtttgatga aagaacttat ttggtattct
    33001 ttccctattt tattagccgg attatccggt actaccaacg aggctttcga tagacttttt
    33061 atcaagtact tattacctaa aagtgtcgac tcgctttacc aattagggat atatggtgct
    33121 tgtgttaaac tagctgtact tttagtactt ttcgtacaaa tgtatcgttt tgctgctgag
    33181 ccttttttct ttaaatcttc aggaagtcag gaccacaata agctttttgg aaagtcattt
    33241 aagtattttc taatcttttc aattgttata tccctcctaa taacctttaa tttaccaata
    33301 ctaaagtttt ttattggcaa aaattatcgt gaatcgcttt atatagtacc tatattactt
    33361 ttagcaaata ttttttatgg tctattcttt aacctttcct tttggtataa gcttgctgat
    33421 aaaactattt atggtttaaa gtacactgtt attggatcaa ttataactat attttctaat
    33481 ataatactta ttccaataat tggcatttat ggtgctgctc ttagccgttt aatcacatat
    33541 gtaactatga ctattatgag ttatagcgat ggtagaacat ctggatttgt ttcgttcgaa
    33601 actaaaaaca taaaatttta tattattttt atattattca ttactttatc tggaacttta
    33661 ttattggtat tcttaaaacc ctatttttca ctaatagcag ctaatatttt aattggttta
    33721 tttatttaca aattcattaa gaaagaaaac ataaaaatta ctttatggag atagttaaaa
    33781 ttcttaacaa atcaacgttc gataacccta catatgctac tgagcattcc agcggtgtcg
    33841 atttaaaagc atttatcgag gagccaataa atttaaagcc attagaaaga aaattaatac
    33901 ctacaggact ttttattgaa ttacctgttg gtattgaagc ccagatccgc cctaggagcg
    33961 gtatggcttt aaaaaaaggt ttatctatac taaacactcc tggcactatc gattccgatt
    34021 accgtggtga aattggagtt attgttgtaa atttaagcaa tgatattgtt accattgaac
    34081 caggcgaaag gatttgtcag atggttttta caaggtatga aaaagtatct tttgaaaacg
    34141 taaacaaaat ttctgaaacc gacaggggtg caggcggttt tggatcaact ggtcataaat
    34201 aatgaaagtt ataataaatt tagttttagt ttttcttttt tctgtgaatt ttaccttttc
    34261 acagaataaa atagatattt tgaataatca aaaaaaggaa atactaaaac aaatagacga
    34321 taacaataaa cagatcgata taattaataa atcaaaaaaa gctacaatta acgagttaac
    34381 cttaaaatta aaatctattg agttacaaaa caggttactt ataaatataa aaaatcaaat
    34441 aacagaaatt tcgaaaaacc taactgagac tacctcacaa gttgatagtt tatctactct
    34501 tattgatact cgaagaaatg aattaattag aatttataaa tcatacttta aaaagttaaa
    34561 gaggaaaaat gatttattaa tacttctact ttcatcaagt agtataaatc aagcttatac
    34621 ccgccttaaa atgtataaaa atcttgttaa ttatttcaat caacaaatat cattacttaa
    34681 tgaaaataag attgaattag aacagaagag gtcaaactat gtgctttttt taaaagaact
    34741 aaaacttaag gagaatgaat accaaaaaac gataactact ctaaataagg aaaaaaatga
    34801 attagaagca actaaaaagg atttagagaa aaagaaaatt gagttaacta aagaaatttc
    34861 caataagaaa aaagcattag cattaattga taatgaaatt aaaagaataa ttgaagagaa
    34921 tgcaagagct ctaaaatcta tgaataaaga aaatgctaag agatataatg aattaagtaa
    34981 tattttcaag gagaacaaag gaaaattacc accacctgca ttaaatagca acgttttaaa
    35041 ctactttgga gagagcaatc acccaatact taaaaatgta aaagtaaaga ataacggaat
    35101 cgatttaata cttaacaatt cttcaacggt tcaagcaatt cacagaggag aggttaaaaa
    35161 aatatttaat gttccatatg ggggaagggc tattattatt aggcatggtg aatatttaag
    35221 cgtttactct aatcttagtg aagtaagtgt aaaagtagga cagcttgtaa atgctggcga
    35281 taatttggga agtgtaatga aacaaaccga taatacactt gttttacact ttgagatatg
    35341 gaatgagaag gaacctgaaa atccgttaga ctggttgaaa ttttagtaaa atcatttttt
    35401 tcataatttt gcgtaacctt tactaaaaaa taaagtataa aaaaataaca acctaaaagc
    35461 catggaacga acaattacaa ttccaagcaa gctagagcaa attagtttaa tcgaaaaaac
    35521 tattgatgaa ataacagaaa aacttacttt aacacccgat gtgtatggaa aaatattaat
    35581 tgctgctatc gagggtgtaa ataatgctat tgttcatggc aataaacttg acgagaaaaa
    35641 agttgttaag gtaaatataa aaatatgcga tactaaagta tgcataacaa tttctgacca
    35701 gggaaaaggt ttcgatccaa atacaattcc ggatccaact attccggaaa acattgaaaa
    35761 catttcgggc cgaggtgtgt ttcttatgaa aaagttgtct gatgaaattg cttttgagaa
    35821 caacggaaca acagtaaaaa tgacttttaa tatttaatgg ctattttttt ttcatataaa
    35881 acaccatcca tctcaaatag gatatccaat aaaaacaaaa taaaaaagtg gataatttat
    35941 attattgaaa aagagtttaa gaaatcagtt ggtgatatta acttctattt tactgacgat
    36001 actgaaatac ttgaaatcaa taaaaagtat ttagctcata actattatac tgatataatt
    36061 actttcaact actgtacaga aaattcaatt tctggtgata ttgttatttc agtcgatacc
    36121 gtaaagacta atgcaaagga ctacaatgtt accttcgaaa atgaattaca cagggtaatt
    36181 gttcacggaa tattacattt gctaggattt gacgattcta cagaggagca aaagaaggaa
    36241 attagagaaa aggagaactg ggctttaaaa attttctact caaaatggaa ttagcctttg
    36301 atgttgttgt tgttggaggt gggcatgcag gatgtgaagc agctgctatt gctgcaaaaa
    36361 tgggctcccg gacactttta gtttcaatgg atcttactaa gttagcccaa atgagctgta
    36421 atcctgctat tggtggaatt gcaaaaggtc agattgttag agaaattgat gcacttggtg
    36481 gatatacagc aaaagtgact gaccttacaa ccctacattt cagaatgctt aatcagagca
    36541 aaggaccagc catgtggagt ccacgcgctc aagctgacag aatgctcttt tcaattgaat
    36601 ggcgaaaata tcttgaatcg atacctaatc tgtatttatg gcaggatgaa gtaattgatt
    36661 taattgtaaa cgaaaaccga gtagagggta taattactaa gacaaatcaa acagttaagt
    36721 gtaagactgt tgttttaaca aatggaacct ttttaaacgg taagatacac gttggaaacg
    36781 tcaattttac cggtggacgt ataggtgaac taaactcaca aggcttaact gagcgtctag
    36841 taaaacttgg tttcacaacc gggagaatga aaacaggtac acctgtaaga attgatggta
    36901 gaaccgttaa ttatagtaaa ctgactatac aagaatcgca agaattatct aaattttcat
    36961 tcaaccccga ttatttttca aaactaccac gtaagcattg ctacatcgtt aacacaaatc
    37021 aggttgtaca taatatttta cgagaggggt ttgatcaatc gcccctgttt actggtaaaa
    37081 taaagggcat aggacctcgt tactgtccaa gcattgagga caaagtgcac acctttgccg
    37141 aaaaaacatc acaccaacta tttttggagc ccgaaggtgt ttttacctat gagatgtact
    37201 taaatggttt ttcgtcatcg ctgccagtag atattcagta caaagcttta aaatcaattc
    37261 ccggttttga gaatgttcat attcttcgac ctggctatgc aattgaatac gattattttg
    37321 atccaacaca gctaaaacat actttagaaa ctaaaatagt tcagaacttg tttttcgctg
    37381 gacaaattaa tggtacaaca ggatacgagg aagcagcagg tcagggttta attgcaggag
    37441 caaatgcaca cttaagggca aatgatatcc aagaaaactt tcagctcagc cgagacgaag
    37501 cttacatcgg agtgttgatt gatgatttgg taacaaaagg tgttgatgaa ccctacagaa
    37561 tgtttaccag ccgagccgaa taccgcatac taattcgcca agacaatgcc gatgaaaggt
    37621 taacgccaat tgcagcaaag tataatatgg ttgacgaaaa cgatgtaaaa cggctcgaaa
    37681 acaagattaa aagcattgag gagctgaatc aactcgttaa agaatattct gtatcgccta
    37741 gcgatataaa cgaatacctt acctcaaata attcagcaaa aattacacaa aaaagaaagc
    37801 tctacgatat tgttttacgt cccgaaatat cgcttaaacc aacacttgag cacctcaaaa
    37861 taaatatcga ctcatacgat tatgaagcca taaacggcgc agaaataaaa attaagtata
    37921 gtggctacat tgaaaaagaa aaaataattg cagaaaaaat aaatcgattg gaacatatta
    37981 aaatacccga cgattttgat tttaatcagc ttaactcatt aagcaccgaa accaggataa
    38041 agttaacaag cataaaaccc aaaactatcg ctcaggcaaa acgaataccc ggaatttctc
    38101 ctagcgatat taatgtatta ttggtatact ttggaaggtg atgttccacg tggaacaatt
    38161 ttttgtttta acataaacat ttcaaaaacc ataagtatat aattattagt gtattaagat
    38221 atttcattaa agtgttccac gtggaacaat ttaacaaaaa gtttaagtaa aacattaatt
    38281 ggatgtttat gaagagttcg aattcaatct cgggaaaatt ggttgaccct atcggtcgta
    38341 gaactgtaaa cgctaaaata tactttcagg atggtgtaat tacccaaatt gatgaatcct
    38401 tggacgctga aaatgtttat ataattcctg gatttgtcga ttcgcatgtg catattgaaa
    38461 gttcattgct tgtgcctacc cggtttgcac aggctgcaat taaaaatgga actgtggctg
    38521 tggttgccga tcctcacgaa gtggcaaatg tagcagggat tgaaggagta cgctttatga
    38581 tagagaatgc aagacaatcc aaactaaaat tcttttttgg agcgccatcg tgtgtgcctg
    38641 catcgcccgt ggatgagtgt tttgaaccct ttacttctag agaggttgca aaactgctag
    38701 acttacctga ggttacccac cttgccgaaa tgatgaattt tcccggagtt attaatggta
    38761 atactgaagt tcattcaata ctaagcgaag ccaaagcaag gaataaaccc attgacggtc
    38821 atgctccggg attgagcggt gaaaatctta ccaaatatgt ttcagccggt attactaccg
    38881 atcacgaatg ttttaccctt gaagaggcta agaaaaaaat tgaacttggg atgaaggttc
    38941 tgattcgaga gggtagcgcc gctaaaaatt ttgatgccct acatccaata attgggcaaa
    39001 acccaagtat ggctatgttc tgcactgacg attgtcaccc cgatgagtta atgggcgggc
    39061 atataaataa gcatgtacgc aaagctattg cgctaggata tgatattttt gatgtgctgc
    39121 aggttgcttc ggttaacccc gttaaccact ataggttaaa tgttggattg ttgcaggtgg
    39181 gccaaccggc cgattttata gttgttgaca acctaaatga ctttaatgta ttatctacct
    39241 atattaaagg tgttaatgta accaattttg aggaagatta tgccgttaaa cccccgttgt
    39301 ttagctttcc aaatcacttt gatcatcaaa aaatcaagct agattttcct tctggcaaac
    39361 cggtaaaggt tattaaggtg attgaaggcg agttgatcac cgaaacctta aaaacatctg
    39421 tacccggttc tggctacgat tttgataaag atattctaaa aattgttgtg ctaagtcgat
    39481 acaacccaaa caatataggg gttgggctta tccatggttt tggaatgcgg aaaggagcca
    39541 ttgcagcaag tattgcccac gatagtcatc atattgtcgc tataggcgca aacgacgaca
    39601 gcatagtgcg agctatagac tatataatta aaaatagggg tggaatatgc tactatgatg
    39661 gaaatgagac tatgggtttg gccttgccgt tttttggttt aatgaccaat gttgatgtga
    39721 gcgatgctgc taaggagtac tcactgatta acaaaaaact taaggccgat ggatgtaagc
    39781 tccatgcgcc atttatgaca atggcattta tgtcgctatc ggtaatacca catttaaagt
    39841 taactccagc tggactgttt gatgttgata ggtttagctt aacctccatt tttgcggaat
    39901 gatgtaaatg gaaaaccttt cgagcatatc ctatttatca tctttacccg atttgcctgg
    39961 ggtttaccaa tttttcgact caacaggcaa aataatttat attggcaagg ccaagaatct
    40021 tcgaaagcga gtatcgtctt attttaaccg ttcccagtat gactcagcaa aagtaagggc
    40081 gttggtttca agggtggccg atttaaagta tattgtagta aactcagaat ccgatgcgct
    40141 catactcgag aatatactta taaaaaaaca tcagcccaag tataacattc ttttgcgcga
    40201 cgataaaacc tacccatgga ttgtaattaa aaacgagcct tttccccgtg taacaaccac
    40261 gcgtcgatac gagaacgatg gatcaatata ttatggacca tatacatcgg gtcgtttact
    40321 taaagcatta cttgatttaa ttcggcagct ttttcccatt cgtacctgtg agctaaactt
    40381 aactcaaagt aatattgcct ctggaaaatt taaagcttgc ttagagtatc agattggaaa
    40441 ttgtttagcg ccttgtatcg gaaatcaaaa ggaaaacgat tatttaagca acattaatgc
    40501 tataaaaaaa attcttgaag gtgaccttaa cgaggtgctc cgcctaacaa cagccgaaat
    40561 gaccaaagcc gctcaggagc taaaatttga acaggccgca ttgcttaagg aaaggcttag
    40621 actgttacaa aatttccagg ccaaaagttc tgtaatttca aagggaataa acactgctga
    40681 tatttttaca attcaagaga gcgaaggttt tgctgttgtt aactacttaa aggtaaacag
    40741 tggcgtggta gttcaatcgt ataatttaca aatcaaaaat attgttgatg agcctattga
    40801 tgatattttt tcacaggcaa ttctagagat taaggagagg cttaacctaa aatctactac
    40861 ttttcttgta aatattaacc ctgcatacca gctgtccaat ataaagtatc acattccaaa
    40921 acagggtgaa aagcttaagc ttttaaagct tagcctacga aatgctgatg cttacataaa
    40981 ggaattaatt aaaagtgagg aacgagcaaa tcctcaaacc cgtgccctaa aataccttga
    41041 gcagttaaag aaggatttac agctaccgga acttccaatg gttattgaat gttttgataa
    41101 ttcgaaccta caaggtacta atccggtttc ggcctgcgtt gtgtttaaga atggaaaacc
    41161 cgctaaaagt gagtataggc attttaatgt aaaaacagtt accggccctg acgattttgc
    41221 ttccatgcgc gaggttgttt accgtaggta taaaagattg attgaagaga aaaagaattt
    41281 gccaaattta attgtcattg atggtggtaa aggacagcta agttctgcct atgaaaccct
    41341 taaagatttg catatcgagc atattcccat aattggtata gcaaagcgac ttgaagaaat
    41401 ttaccgaccc ggcgacacaa ctcccttgta cctcgataaa cgttcaatgt cccttaagct
    41461 tattcagaat attcgcgatg aagcgcatag gtttggaata acatttcaca gaaagaaaag
    41521 agaaaaaaag aacgaagcat cgatactgga atcaatacct ggtgtaggaa agaaaagcgt
    41581 tattaagctt tacacccgct ttaaatcatt agaaaatata aaaatggcct cccttgaaga
    41641 gctttcggaa gaggttggag caaaaacagc cagcaatatc gtgtcgttct ttaaaaaaat
    41701 ataggtagtt ttgcaaaaat attctgtatt tatgtcagcc tttcaatcga gccagaatgc
    41761 taataattta tacaacaact ttttggattt aacccagaag tacgattccg gaaagcttta
    41821 tactatccac gataattttc ctttaacttc cgattcttgt tcaaatgttt ttaagtcaag
    41881 ttcatttaaa tcaaacatca ccattgaacc taaacccctt aaccataatc atttaattcc
    41941 gaatgcttat tttattagca tggttattac aatgctgata atttcatcaa taatagcagg
    42001 ttacgcaaaa tatcttagag tttttattca atcactattc tacgatttta ttgctgaaaa
    42061 aacccttaac gattatagcg tgccctttat taagctttct cgtttactgg atttcctatc
    42121 aataatatca ataacatttg tagttaatat tatactcgag caatttttag gatcaatgca
    42181 gggatcctta ctctttttag ctttacccgc ccttgcactt atactttaca gaatttggat
    42241 ttggctacta cataaaactt tattgctggc tactaataat gcaagccagg ttaactatct
    42301 ctattttcac aatactttga atttgagaat tttgatgctc tttcttatac ctacatcgct
    42361 tgcagcaaac tataccatgc cgcccttacg aatagtattt atctatatat ctattgcgtt
    42421 gattataatc gctttactat atcgatattt ttactttgta aaaatcttta taaagcatag
    42481 ggtttcatta ttgtattaca ttttgtacct ttgtgccctt gaattgccta taatattagg
    42541 ggtaacgtat ttgctgagag tgagttaagt ttttgttggt tttaaaaaca tagggccttg
    42601 aaaatcaaaa agattgttat ttcgcaaccc gacccccaga gcgagaaatc gccgtataat
    42661 gaattgtcgg agaaatatgg ggttcatctt gatttccgcc cttttataca tgttgagggg
    42721 gtaagttcca aggagttccg tcagcagaaa atcaatatcc tagatcattc tgctgtaatt
    42781 tttaccagta aaactggcat tgatcatttt ttcaggatat gtgaagaact tcgcataaca
    42841 gttcctgaaa ccatgaagta tttctgtata accgaatcaa ttgctcatta cctgcagaaa
    42901 tacatagttt acaggaagcg taaaatcttt tttggcgata acactttcaa agatctactg
    42961 gatataatcc ttaagcataa ggacgaaaaa tttttattgc cactttccga tccacacaaa
    43021 ccagaaattc caaaggcatt aacaaaggcc aagataaatt ataccaaagg gatattctat
    43081 aaaactgtta gcagcgatct gtcagacctt aagccattcc cttacgatat gattgtgttc
    43141 tatagcccta atggaattaa atcgctattc gataatttcc ccgattttca gcagaacgat
    43201 atagcaattg gcgcctttgg cgatacaact gcaaaagcag tgaaagatgc gggtttaaga
    43261 ctcgatgtta aagctccaac cgatgcgttt aaaagcatga cccaagcctt agatcacttt
    43321 ttgaaagagc aatcaaagaa aaagtaaaat tatagagggg ggaatgctac cccccttttt
    43381 tgtatgcctc aaaaacttag gaaaaagtat ataatccatt tggaaaacga ggttaactcg
    43441 ctgttaaaat cggagttatc acgtttccat tatccaatca agttaatata ccaagtagat
    43501 gaaaggatac ccggtggcat tgaggaaaac ccccactaca aagtaatatt tataattcct
    43561 aagaggtatt taaaaaaagc atatatgaga aatcttgcaa aacgaaggct tagagaggct
    43621 tttcgcatta atcaaaactt acttgaccaa ttcagggaaa gaggaaaaac ggtttacctg
    43681 gcttttatct atgtttcagc agaggttgtt tcttttaaac aattggaatc gggagttgtt
    43741 aaactgttaa ccgatcttaa gtaaaatgaa tttggttaaa acccttaaaa tattatggaa
    43801 atatgcggtt aggcttttgg cctacattcc tattctatta ataaagattt accaaatatt
    43861 tttatcgcca tacctaccta acagctgcag gtttacccct acctgttccg cttacagtgt
    43921 tgaagcgcta aaaaaacatg gtttttttaa aggcttatgg ttgtcggtta ggagaatttc
    43981 acgatgccac ccctggggtg gtcatggtta tgatcctgtt ccctaggctt ttttgattat
    44041 aatcctaaag gttgaaccaa ccccaagctc cgaacgaaac acaaagattt ttcctgagtg
    44101 gtactgttca acaatacgtt ttgctaaact tagcccaagt ccccaaccgc gttcctttgt
    44161 ggtgtagcca gcctggaata tttctttttg cttccccttt tcaatcccct taccttggtc
    44221 ctgtatgtca ataataacgt tgtttttttc aacagttgcc tctacgctaa taactccttt
    44281 cccttgcata gcatctacag catttttaag aatattttcc aaaacccaac ctagaagtat
    44341 atggtttgct ttagcatata ttggaatgcc ggggctgaag ttgaaagcaa ttgtctttgg
    44401 gcatcttttg ctctggtagt ttatggaatc ggtaatcaaa ttagttatat caatactatc
    44461 gagacttggt gatgagccaa tctttgagaa tcggctagtt atggtttcaa ttctttttac
    44521 atctttttca agttcagtca ccacttcgtc gttggggtat ttctcagaaa gtagcgatga
    44581 gcaagccatt agcgatgaag taggagttcc aagttgatgt gctgtttcct ttgccatccc
    44641 gagccagatt aaattctgtt ccgatttacg tgcctgacca taggagtaaa acgctattcc
    44701 tataaagaat gcaattataa taagttgaat ataaggatag taattaagtt tttttaaggt
    44761 aatactatca aagtagtaca catagttttt tgatccatcg ggcagttcaa ttgttattgg
    44821 atcgtgttca gccctcattt tgtttagaag attcgaaaca taaacagtat cctggatgtc
    44881 aggtaattca atattgcgga aaccaatcac tttcccagtt tcatcggtga gtattactgg
    44941 tacagtccgg ttgttttgaa ctatctcaaa aatcagtgaa aaatcctcac acgaacccat
    45001 ttcgtttgag atgtatttaa cggcatttgc ccataggtca attttttgac gctcctcctg
    45061 cttaagttcc gatacaaaac gatttgtaaa gtagattgta ataaacgaaa cggtaaccgc
    45121 tgcgattatt agtgttaaca gaaatttatt tcgtggacta agaattttca ttttactcgt
    45181 tctttagggt gtcaatttgc tcatcccact ccagcatgta acccacacct acattaccat
    45241 cagttttttc cttaggtttt atttgaggac tattttgtat agaaacgcta tcggtttgaa
    45301 cggtattgtg tattccggat tcaaaacctt tcttgtttat ccattcaacc tcatagctat
    45361 cgttttggcc aaatagtttc aagtataaat ttatagatcc atcggtagtg gaatgaaaat
    45421 caatctgatc ggatttcaca aactttcgtt ttaacaaatt gcttatgtta atcctagcaa
    45481 tatactcata tctgttgtca aaatcgtgaa ccccagaaac attcattagc aagacattgg
    45541 attttatatc catattagga ataaaaatct tacgattttc aatagtaatc ttatttttca
    45601 aggtatcaaa agcaataacc ttaacctggt ccaggtttag ccatttggac aattccttaa
    45661 ttctatccat gccttttagt tttccttgaa ccacttgaac atcggagaaa annnnnnnnn
    45721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    45781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nttaattcta tccatgcctt ttagttttcc
    45841 ttgaaccact tgaacatcgg agaaaagttt aagagagccc aagtcaacat cgcctttctc
    45901 gttttttttg aattgcatat tcattatgcc tttaaatcgt ccggaaagat tatcagctgt
    45961 tatgtagctt tgaccccaat tattgaaggt tttgaaaaca tattcaatag aaacattgct
    46021 aacccaaagg tttgtgtgaa ctgaattgct atgtgattta aatctaccag aaacgttgcc
    46081 atcaaagcaa tcggcagtaa agtaatctgc tgaaattgta tcgccattaa ttattccgtt
    46141 ggcttttagg ctattaagtt ttaatcgatt gtatgtagct cttttaaaat tgagcataaa
    46201 tgacattttt ggtaggtcaa cccctttggt agatgtattg ctagttctat cagtaattag
    46261 atgaactata tcgttgtaat ctatatggtt accgtaaaga tatatgtttg gtgaatacct
    46321 attttgggta taggattcca ggaaattggc ttgattaata ttaaagttta tatcggttga
    46381 atcggattta aaatagcctg caatgtttaa atcgttatta atcgctatag ttcctttaac
    46441 cgaaggctga ttaaatgaaa tgtttttctt gttttcgaaa actagatcgg atttaagaag
    46501 taggggagta atactttccg tttttggggc atacgatatt aatgctttaa attgacctgt
    46561 tgtttcaata tcaatttcag gtttttgtat tcttaaattg tggcttacat atcccttagc
    46621 taaaattacc aagttattga acccttttat ttttgcgttt aagttaattt ttgagttatt
    46681 actttcaata gatgctttag gaatatagct gaagtgcttt gaaaaattgt tagttagtaa
    46741 agtataaccc tccaaattgg ttatggttgt ctcttcgttt ttagttatcc atttcaattt
    46801 attgctatta tagttaacct gaaaggtatc aactttttca aaacccgatg atagcctaag
    46861 gtatgcatct gctgtaatta taccctttat ttggtcaaaa ctcttaagat aaggcaagta
    46921 cgatagatct tgtgcttgaa accggtttga tttaaacctg aatgcggcta attggctatt
    46981 aaagtcataa attcccgaac cgtttatacg taatgattta attgatgatt ctaaattata
    47041 gatatacaac ttattatcac tattttcaat atcaaaattt agctgatgct gattggtaat
    47101 tttaaaatat tcacctacgg tagttttact cagctcgctt tgaatggata atagcaactt
    47161 cgtatgctcc gttttgaact tgatgtttaa gtttttccct gagcagtata gcactgtttt
    47221 actaacgcta tccaacaatt caaaaaagaa attattaaga ctaatccccg aaatcaggac
    47281 ggaaacagaa ctttcagaac tactatcact gctaccgaaa agttcttcag ggtaattggt
    47341 ttgccatttt attcttgcat tgcttactct tatattttta atatcaattt taccacctag
    47401 taatttaaaa ggggagatgc taatcagaaa atcatctgtt tttaaaacag tgtcggattt
    47461 agagtttagg ttaattacta atacaaaatc ggtaaatgag tatgaaaacc ttggaaatgt
    47521 tttaagggta ttaaccgaaa cggaactata ggttaaggat aggtcgggat acttgttaag
    47581 attttcccta agggtgttta taatgtattc ttgcttgaaa aacaaaattc ctgcacctgt
    47641 gagcaccaga ataagcatca gtagcaggag caaaataatg attctaaaaa ttcggtgata
    47701 tatttttacc aatattgcca tatatttgtt actagttggg tgtaatttta tttgttgcga
    47761 aattatgcta tctttccaaa tgaaggaatt tttttgccat gatgtttaaa aagatattat
    47821 tgccagttta tgccattctg tgtttgcatt tggttgcaca gccgcagaat gccgactcac
    47881 tgctgcttca actataccaa gaatcgaacg acagcttgcg cgtactcatt ctacaaaaaa
    47941 tagccagggt ttatgaagta aatgagaaca accctgttct agcagaaaat tacctacttg
    48001 aggctaaacg cttggcggaa tcaataaaaa gcaattacct ccgaatggag gtatatacct
    48061 cgcttggtgt gctataccga aacatttcaa actacgcaaa tgcattaaag tttcataacg
    48121 atgcgttaga tcttgcaaga gaaatcgacg ataaacatca acaggcaaaa tcgctcaata
    48181 accttggagt ggtttatcgc cggctcgata accacgctta tgcctctgag tatcatctaa
    48241 aagccttaaa gttggccgaa gagataaagg acacattcaa tatttcagtg gcctgcaata
    48301 gccttggcaa cattttttcg ctaaacggac gctacgatga agccttggaa tactttcaga
    48361 gagccctcta cctctcccag caaacaaata atctgcttgg ccaggctatg aactttaata
    48421 atattggcga ggtttacgaa tttaaaggtg attttattaa ggcaaaggat tactactcaa
    48481 aatcgcttga tatcaattac caaatcaaca gccaaaaagg tatagctatc aactttaatg
    48541 ctttaggtaa aatcgaactt tacttaggaa atccattaaa ggctcaagat tattttacta
    48601 aggcgcttgt aatagataaa aagctcaacg ataaaaagtt tatagccgat agctacatta
    48661 acttagcccg agcccagtta ggattaaaca atgataggag tgcctacgaa aacgtttttg
    48721 catgcctcag tattgccaag gaaataaaaa gtttaattca ttttcagcaa gcgtatgagg
    48781 ttttatcgga ttactaccaa aagcaaaatg aatatagcaa agcgcttgag tactttaaac
    48841 tatcatcaag ctacaaggat agcgttttaa acgaaaagaa tagcaggcac atatctacca
    48901 tacaaacact atacgagaca gaaaagaaag agaatgaaat taagctatta cttcagcagc
    48961 aagagttaaa gcaacgggaa attactagaa ctaaaatcca aacctatgtc ctattgattg
    49021 ggcttttgct tagcggggcc atgttgctgg ttatttactt tgccctgagg gataagcgaa
    49081 gaactgccga aaagcttgca aggcagcttg aggagattga gctaaaaaat gtgttactcg
    49141 aagagaaaaa tcaggagatt gagcaacaaa agcaagaaat tgaaaaaaat aaggatttca
    49201 ttgaacaaaa gaacaaaaac ctggaagaag cctata
//
LOCUS       DAOSAL010000012        45267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_614_length_45267_cov_48.894320, whole genome shotgun sequence.
ACCESSION   DAOSAL010000012 DAOSAL010000000
VERSION     DAOSAL010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 45267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45267
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_614_length_45267_cov_48.894320"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1974)
                     /locus_tag="PK049_03130"
     CDS             complement(<1..1974)
                     /locus_tag="PK049_03130"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOK60581.1"
                     /translation="MKRLLLIALGLVYLTSGNISAQPTKKLEKADALYNAGAYFDAIA
                     LYRDDIDKIKDKKELSLYLFKIGNCFRQIGEPRQAEQWYEKAIRRECPNPKVYYYYAE
                     MLKMNEKYDLAIEQYQKYKVLVPNDPLAEVGIQSCELAKQWIATPAGYEITNMRAINS
                     KFSDFSPAYASTDYNILYLTSSRNGTIGNKTSAVTGENFSDIFITELDNKGVWSAPKP
                     LDETINSQFDEGTPSLTPDGNNLFFTRCKVSKRNKLGCQILYAKQSDQGWLTPKELEL
                     AADSMIVAHPAISADGTELYFVSNIPGGNGGLDIWKITREAGSEEWSAPINLGPQINT
                     PGNEMFPYIHPDGTLYFSSDGHPGMGGLDIFKAKKNDEGQWVIENMRYPVNSTADDFG
                     IIFEKDREAGFFSSRRNGGRGADDIYMFYLPPINFNMIGKVMDEKTRTTIADATVKLV
                     GSDGTIATMQTQSDGSFRFNLVPNTDYVIVASKKGYLNNKAKETTKGLTKSQDFNVSI
                     ALTSTAKPIEIPNIFYDYDKWELRPESKEALDNLIQILNDNPNITIELASHTDSRGSQ
                     EYNYELSQKRAQAVVNYLIENGIATDRLKAKGYAQTQPKVVDNNLAAQYPFLTPGTVL
                     DKKFIDSLTSEDQKETVHQINRRTEFRVLREDYQ"
     gene            complement(2278..4473)
                     /locus_tag="PK049_03135"
     CDS             complement(2278..4473)
                     /locus_tag="PK049_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HOK60582.1"
                     /translation="MPKLRFRAFGEATNRIPVDMNGEGKAESLVDIFAINVFDRPKMK
                     KYLSEEAYESIITSIDEGKKIDRKIAGQVASAMKAWAIERGATHYTHWFHPLNGSTAE
                     KHDAFLSIDIKGQPIEVFKGDMLVQQEPDASSFPSGGIRNTFEARGYTAWDPTSPAFI
                     LENTLCIPTVFVSYTGEALDFKTPLLKSLALLDKAALSVLEYFDKDARKVYATLGWEQ
                     EYFLIDESLYNARPDLALCGRTLMGHAAAKDQQLEDRYFGSIPARVSAFMRHFEVEAL
                     KLGIPIKTRHNEVAPNQFECAPIFEEANLAVDHNQLIMSLMDRIARQHGFRVLFHEKP
                     FKGINGSGKHNNWSMMTDTGTNLLSPGKTPKNNLQFLTFLINTIKAVHDHSALLLGSI
                     ASEPNSHRLGAHEAPPAIMSVFIGKTLSAVLDELEQKVTDQKMTPDEKTELKLEIIGK
                     IPEILLDNTDRNRTSPFAFTGNRFEFRAVGSSGSCASPMIVLNTAVANQLMKFKDEVD
                     KVIAKGIKKDEAIIQCLRKVIIESKKIRFEGNGYSQEWVDEAARRGLPNISNPLEAFK
                     TFLLDSSVKLLTGNGILTKRELEARYEIRNEIFVKKVQIEARVIGDLAINHIIPTAIA
                     YQNTLISNVKGLKELFPNEWEKLADHQHALILEISEHIHAISHQVNEMIERRKEANQV
                     EDIVEKAHLYGKNVKPYIESIRTHIDKLEMIVDDEMWPLPKYREMLFIR"
     gene            4821..5303
                     /locus_tag="PK049_03140"
     CDS             4821..5303
                     /locus_tag="PK049_03140"
                     /inference="COORDINATES: protein motif:HMM:NF018595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HOK60583.1"
                     /translation="MESDNRKYSNDEITVFWRPGECVHASICYTKLLSVFNPRKRPWV
                     NMNGASTERIIEIVNECPTNALTFMWNDPEKNKQERSHKCVREFTPEEVEAFEVTPVK
                     IQVMPNGPLLVSGDFQIIDADGKEVKSMKMVSICRCGHSNSQPFCDGTHFKKGFRDRE
                     "
     gene            5290..5514
                     /locus_tag="PK049_03145"
     CDS             5290..5514
                     /locus_tag="PK049_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HOK60584.1"
                     /translation="MIESSKMNQPKKIAAKLHLSKGGPIRVEGRFFIVNLDGESLIPD
                     NSKEVFLCTCGASRNKPFCDGSHKGGKSNG"
     gene            5654..6049
                     /locus_tag="PK049_03150"
     CDS             5654..6049
                     /locus_tag="PK049_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019149742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HOK60585.1"
                     /translation="MELHKARKAKSPVLRALFVIFGIVSFGLGVLGIFLPLLPTTPFM
                     LLSAFLFIRSSGRLYRWLINHRLFGKYIHDYIERRSIPRRVKWYTLGLLWASILTSVA
                     TLDVGNVIRLILLLIAVGVTAHVVKLRNS"
     gene            complement(6056..7048)
                     /locus_tag="PK049_03155"
     CDS             complement(6056..7048)
                     /locus_tag="PK049_03155"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOK60586.1"
                     /translation="MNTKLNIAIMAGGNSSEANISMRSATQIAGWLDPEQFNAYKILV
                     KGTTWTLEHPQKGQIPVNKNDFTVDIDGQKLSFHCALIAIHGTPGENGLLQAYFEMLQ
                     IPYTTGGVMNSSVTFNKYYCKELVRETGVNLAKGLLLRRGQKVDAQAILDTLGLPIFV
                     KPNESGSSYGVSKVKQASDLMPAIEKAFAEDKTIILEEFIPGREFTCGVFKYQNGEMI
                     MPVTEIVPETEFFDYEAKYRNKSKEITPAPIPESLTSAIQQFTSKIYDRLQCHGVVRV
                     DYIVNNDKIYFLEINTVPGMSEASIIPQQVKAMGGNMRQFFTLVIYDALNRSRE"
     gene            complement(7145..8128)
                     /locus_tag="PK049_03160"
     CDS             complement(7145..8128)
                     /locus_tag="PK049_03160"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HOK60587.1"
                     /translation="MADLSTTYMGFKLKNPYIASSSGLTDSVEKAALLQKHGFAAIVV
                     KSLFEEQIMMDVDAQRMNNMFNSYSEAEDYIAYYTRKHALSNYVELIDGMKQKLSIPV
                     IASINCSSADSWIDFAGDIQAAGADAIELNMFIMPSDVKMKGDEIEKIYIDVARKVAQ
                     TLSVPVAMKIHHYFSGMANFAVELSRTGIKALVLFNRFYQPDVDLNTLKVTSGSILSH
                     PDENAEVRRWLSILSGRVACNLAASTGIHDWQTAVKNILLGANAVQVASAMYKSGPEI
                     LEPWIEETSKWLDANDFESINSITGKLRQADSIKPLAYERAQFMRYFSDAR"
     gene            complement(8160..9959)
                     /locus_tag="PK049_03165"
     CDS             complement(8160..9959)
                     /locus_tag="PK049_03165"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOK60588.1"
                     /translation="MARIKDIISPFTAWKNVVKEPVTIKEPLKREAAPRYRGFHKNDV
                     DTCIGCGTCESICQNAAIDMVPVEGIETKNGDSGLRPRIDYGRCCWCALCVDVCTTGS
                     LSMSNHFKWVDSDPDVFRFIPGVDIKEWDDEPNGYKKPGPNYELYSKERVHMPELKPE
                     ERDKSFIEIVRGYSREQAIKEADRCVECGICVATCPAHMGIPEYIKAIRNDDLNEGLR
                     ILYDTNPLPEVCGRICTHKCETVCSVGHQGDPLSIRWLKRYIADQQPAENYKQILETE
                     NISKNGKRIAIIGAGPAGLSAAHYLALMGYECTIFEKLPLPGGMMRYGIPEYRLPYDA
                     LDKDIDYIKSLGVEIRCNTTVGKDITLEELHNQFDAVFTGTGLHLGRSTRIPGTDHPH
                     VYQAVDLLRDVTMGKEIPAAPSIVVIGGGNVAMDITRTLARIQNQRYGKVNIITTSLE
                     SENEMPADREEVVEAREEGATIIPGWGPVKIEIENNKIKGLHVVKCVSVFDENRRFNP
                     KFDDTQTDFFAGEMVVESIGQGMDLSYLPESIKDNLKLDSRGRIVVNDYFQSSVEWLF
                     VGGDIIQGPDVIHGIANGHKAAIGIDRFLGGKK"
     gene            complement(9982..11175)
                     /locus_tag="PK049_03170"
     CDS             complement(9982..11175)
                     /locus_tag="PK049_03170"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HOK60589.1"
                     /translation="MKFSKLFKINEDRSLYPPLNSEGKVDIDLSSHKYVKLWHGPQHP
                     GITGNMSLELTLLGDEIMDCETHVGYLHRGFEKLMERRKWIQCFPIVCRITVPEPDFN
                     EYCFAAGVEELAGIEIPETAQWLRTLTLEMARLQSYLMWIGGQAGSLGMGIIGQWSNY
                     CRDLVLDRFEELTGGRIYHMYIIPGGVRGLLPDGFEARMEETLKTIEEFMVDIDRVMF
                     NNAVFKKRTVGMGYIDPKWIYQFGITGPNARAAGIPDDVRKKYPYLKYPELDFEPVVG
                     KDSDIYTRADVRRRDLLLSVDLIRQILAKMPKDGDFKASTPNVLHWQIPAGETFIRSE
                     CTRGEFAYYIVSDGSEYPRRINVRGPSYTHAVALLEKMIINTNLADVAGLMTSLHTYP
                     PEIER"
     gene            complement(11165..11695)
                     /locus_tag="PK049_03175"
     CDS             complement(11165..11695)
                     /locus_tag="PK049_03175"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOK60590.1"
                     /translation="MNTLLDNIKTVFPNAEVWTQRKGLYHMSIPKEQLVTLVTHLKEV
                     EGYLHLVQLTAVDWIEKNQFQLTYILNNPNKRIDVMVKTFIPRDNPTMETIHHLWEQA
                     ATYQRELREMFGIDFPGSPRVDKPFILEGWDNIPPYRRDFDTKKYAEETYFPRPGRET
                     HDPKEYMKKKLYPDEI"
     gene            complement(11707..12348)
                     /locus_tag="PK049_03180"
     CDS             complement(11707..12348)
                     /locus_tag="PK049_03180"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HOK60591.1"
                     /translation="MSKSDLEKRTVMSPDGEPIEINPLQDYFCDARPKVHEPVLPIFE
                     KFLNWARSESLWVLGFGTGCGAIEMRPLMTPRFDAYRFGIQWRPTPRQSSVFIISGYL
                     SVKTLKRVIRSYEQMQNPKFVIALGSCTINGGMYWDSYNTIKRLDHYLPVDVYIAGCM
                     PRPEAILAGFEKLKELIRQGKGEGANLYAKNFDWYKANQKKIIKDWDIPDYNW"
     gene            complement(12377..13282)
                     /locus_tag="PK049_03185"
     CDS             complement(12377..13282)
                     /locus_tag="PK049_03185"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOK60592.1"
                     /translation="MEFGIKQILYLLLTLFIVLNYGLILQGIMPKIAARVGRRYGIRW
                     YQPYLDLIKNHAKRSKITHGVMYYLGPVFRLTGGIGLLFFLPVIYGSPMFDNLSFAGD
                     LTLVLFFQFFGVLGMALGAGEGGHPYSAIGVSRGLSQVTALELPLTLGVISIAMQYHT
                     LNISDIVAAQQGGILNWTIFTNPFAGAALMLTFLGSMGRAPFDVVLAPQEIPIGPPTE
                     YHSGFLGVLQLNRAIFPVAKLILFMNLLYGGATSWPELFIKTFFIYLWSIFVGVAFPR
                     FRVDQSVRWFLGVPLLFGIIAILLI"
     gene            complement(13283..>14623)
                     /locus_tag="PK049_03190"
     CDS             complement(13283..>14623)
                     /locus_tag="PK049_03190"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOK60593.1"
                     /translation="GAYLILAGFSIAQLGHTGMGLEILKDIHYYAPIAFTLMAIGFMT
                     KLASIGLHIWLPGAHAEAEADVSPMVSAILLKAGVLGLVMLFLSMGPQKLFGIDVLYA
                     LSWVGVLTALMGNMMAAFQEDAKRLLAYSSIGVLGYALFGLTMMSHLGWLASLSIVVT
                     HFMFKSLLFLAIGGVVMRTKTKYMYQMGGLIKQMPFSFVSVLIGIIVIAGLPPLTGFG
                     GKWLMFNAIMQNGWYFQAGIAFFAGVISFLYCFRLIHTIFLGQPKDELRNVKEAPFWM
                     LVPQYIIITVLLVLSALPNSMLKPIGQILLNYFPEGALVWHGQLAIGPFGYWNGKLIL
                     AIVVLIFGAVFAWLYIINRKAQKVKQFNIVYSAERPFRPETTHFAYNFYAPYKKAVGF
                     LVAPGITNFWDAVGEGAHAIADKIRSIYNGNGQTYLVHILGFTVIVYILINGGF"
     assembly_gap    14624..14723
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14724..16563)
                     /locus_tag="PK049_03195"
     CDS             complement(<14724..16563)
                     /locus_tag="PK049_03195"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOK60594.1"
                     /translation="MVNPIHIVTIALGVAFALGFLGKREKLALGVVYTALAAMIAISA
                     GWLSHFWGNATESVQIFTGGFKPPFAINLQMGLAESIFTLLINTVGLLGAIYLTKELL
                     QAGKNMMIVFLVLVLGLNVMVMTRDLFNLFVFMEIQSIAIAGLVILNPDRKSIPAGFK
                     YVIATGLISGLLLLGTVFIYYFGGTLNIDFISQASLLYVKGGYVAIFLVLLSIVLELK
                     PFPANGWALDVYEGAHPGIGALISAASATAGIFVLDKLMVFGNFTLFNALSIIGAITF
                     MASNLLGIKQENTRRLLGYSSVGQIGLIMTIIGLKPYLGENTKFIALAILLSHYLAKA
                     GLFWLAGIVNVDKLKDWGAIRGHRIYLFLMGTFIFTLVGFPPFPSFFGKWQLVMQLAS
                     NHHFWLLAIILLGSLFEGVYLFRWLGYAIKWDEVEQNYPKIKLHQFIPTALFAGLTYA
                     LGFYFGEITGYKSTFIYMATAFIAFLYIVDFLPVYIKNTLAIGAMVLYYFSHQDTFHG
                     LTSIFFIIFVIGGIITLLAGYAFKGKRTGFYPLVMMMFAGLIGLLEAKTTLEFFFAWE
                     LMTAGSYFLIIRGKKSMEHALSYILFSVGGAYLILAGFSIAQLGHTG"
     gene            complement(16557..16931)
                     /locus_tag="PK049_03200"
     CDS             complement(16557..16931)
                     /locus_tag="PK049_03200"
                     /inference="COORDINATES: protein motif:HMM:NF012636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter subunit C"
                     /protein_id="HOK60595.1"
                     /translation="MAQYLTLEFLAMLIGFMLIVVGLWGMITQKNLIKIVIGFSLFDT
                     GLNIVIVSMAYIKNSTAPIIDKAVDVSQAASKVADPVPQALVLTSIVIGLGVTALMLA
                     YVLRMYREKKSLDINSYTDLKW"
     gene            complement(16934..17635)
                     /locus_tag="PK049_03205"
     CDS             complement(16934..17635)
                     /locus_tag="PK049_03205"
                     /inference="COORDINATES: protein motif:HMM:NF006248.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter subunit B"
                     /protein_id="HOK60596.1"
                     /translation="MIKKGILLLLLVGFVAVIGSLLINFTGSNELNPLARYYAENGPA
                     EVGAANLVTAVVVTYRGFDTLGEVTILFIAAAIVGFFLKVMNADNGRPNKLRKTSEIL
                     ETASTLLVPTIFMLGVYIFVNGHLTPGGGFQGGAVIATGVMMIILAQPTSRFNHKLIS
                     VLESVSGVGFVAMGILGLILASGFLNNAFLPLGKLGSLLSAGAIPIIYVFIGIKVGSE
                     LTGILDSLKENQNEI"
     gene            complement(17628..17870)
                     /locus_tag="PK049_03210"
     CDS             complement(17628..17870)
                     /locus_tag="PK049_03210"
                     /inference="COORDINATES: protein motif:HMM:NF024641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HOK60597.1"
                     /translation="MSLILAIFLGVVILVLAVTAIYHKNTKVAIIATGAVGLFASVLY
                     LLLAAPDVALTEASIGSGLSTIIFFYVLNKIRHSND"
     gene            complement(17873..18214)
                     /gene="mnhG"
                     /locus_tag="PK049_03215"
     CDS             complement(17873..18214)
                     /gene="mnhG"
                     /locus_tag="PK049_03215"
                     /inference="COORDINATES: protein motif:HMM:NF009314.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HOK60598.1"
                     /translation="MEIRELIGASVTLVGSIFIFLGGLGIVRMPDLFNRIQAGTKAST
                     LGTILSLIGLAIVNPAWIWKLLVIMFFVLITNPVSSNVISRAAHFRGTKMTDKTVADL
                     LVESENKTEEE"
     gene            complement(18214..18474)
                     /locus_tag="PK049_03220"
     CDS             complement(18214..18474)
                     /locus_tag="PK049_03220"
                     /inference="COORDINATES: protein motif:HMM:NF015993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HOK60599.1"
                     /translation="MVNTILIMAGVLMALGILLALLRFIKGPDVTDRTIAFDVMTVAS
                     ISVIALISHFAGRIIYLDVALVYGLLSFLSVVVIGRYIERGL"
     gene            complement(18467..18961)
                     /locus_tag="PK049_03225"
     CDS             complement(18467..18961)
                     /locus_tag="PK049_03225"
                     /inference="COORDINATES: protein motif:HMM:NF014012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HOK60600.1"
                     /translation="MNIRNVANLFVLLFLIWLLLTGTFELTSLLLGLALAVTLALIFG
                     KNSNVFGRFRMTPKVFVYSLIYLFVLSWEIVKSNIDVALRVLNPKLPINPGIVKVKTR
                     LKSPIGRMILANSITLTPGTLTIDMKDDELYIHWIDVKTLDTEEASRKILGKFEKLLE
                     VIYG"
     gene            19161..19850
                     /locus_tag="PK049_03230"
     CDS             19161..19850
                     /locus_tag="PK049_03230"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOK60601.1"
                     /translation="MVESKKSCLLCNNKSECFRQLSDSQLDFANSNRAEISYRKGEMI
                     AKQGNFYTHILYLQSGMVKVYKELPDGSNLILSIFGVGSLIGLPFLFKNQILDYSVAA
                     LDDTTICAIDRHAFEKLIHENGEFAAEVVNQLNQCTLYNYDRLVSLTHKQINGRFADT
                     LLYLSRVVYKANPFNLTLSRKDLAEYTGVSVMSVIRAIKEFKNDGIIDISGNRVEILN
                     FSQLERISRTG"
     gene            complement(19947..21002)
                     /locus_tag="PK049_03235"
     CDS             complement(19947..21002)
                     /locus_tag="PK049_03235"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOK60602.1"
                     /translation="MNQNRLLDPDDDLENGTAEQADLYEHFRIEVDKGQTPTRIDKFL
                     TDKIRNVSRNRIQGAADAGNILVNGKAVKSSYKVKPLDTISIVLAYPPRETDIKPENI
                     PIDIVYEDEALLVVNKPAGMVVHPAHGNFTGTLVNALLYHLRDVPLFKSGEVRPGLVH
                     RIDRNTSGLLVIAKTEMAMNHLARQFFERSTDRLYIALVWGDMETDSGTITGHIGRSI
                     RDRKKMQVFPDGEQGKHAVTHWKVIERFGFVTLIECKLETGRTHQIRAHMEYIRHPLF
                     SDEVYGGDKILKGNLTTKYKQFLENCFELCPRHALHAKTLGFTHPLTNKKMHFDSQLP
                     YDMSALIEKWRAYGKGI"
     gene            complement(20999..22912)
                     /locus_tag="PK049_03240"
     CDS             complement(20999..22912)
                     /locus_tag="PK049_03240"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOK60603.1"
                     /translation="MRKALTILVFIFVSSLCYGQEVLVNLSGYRTISDVTKKSNYTKT
                     NLSLPFFDDFARNTPYPTPELWEPSNAISNKTYAINPPTVGAVTFDAVNRKGVLHQHL
                     TTTPQPADTLTSLPINLNYPATDSIYLSFNVQPGGLGYQPSPNDSLVLELYSPSENRW
                     VRGWASSVDFNANKIAFYNHLLNNKDEKSSTKLDSTFFTVNINIDNPIFLENGFRFRF
                     IGYASLMENATVPGYKTNSDHWHIDLVYLNRLRNWDDTIYNDIAFRKPIKSMLKNYTS
                     VPWTHFADAEASEIKNPREFSIVYNNLGPTTWNVTRRFAVTNLSTGNEYAFSGGAENI
                     YGYEEFTYTRPFDYTFTSAWPDSAKFMLKTFLQTDFDESTKHLRYNDTLVHQLNFYNY
                     YALDDGSAESGYGLFGEGTINAMIAQKFYSYKADNLVGVMIYFNRSYQDANNQPFKIS
                     IWADNNGKPGELLYQRSTTRPLFTDSLNCFTVYRTDPVSIPVGNFYIGWQQSTIEFLN
                     VGFDRNTNSRERIFHTLPGYWTNTQFEGTIMIRPIFGKLYQNPTTAPISTTSNIRVYP
                     NPASEKIYIDVGNGIAVNSYQLISITGQVIESKSVYQNEPIDVEHIPTGMYLLKVNLA
                     NGQSLTSKVIIKQ"
     gene            complement(22920..23798)
                     /locus_tag="PK049_03245"
     CDS             complement(22920..23798)
                     /locus_tag="PK049_03245"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOK60604.1"
                     /translation="MTTKTAFEKVKQYLLKIYKIPYVNTIINGLGLILGVLIVLMIFL
                     RIYTRHNQEQPTPTLLGMAVEEAAEVIADNNLRIEIADSVYIFNKKPGSVISQNPEPG
                     TMVKKNRRVFVTINAKNPIKIEIPNIVGYTLRQAKAILEQEGFEVGTLYFRPDLGLNN
                     VLEQRFEGKIAEPGTRIPKGSKIDLVLGQGMQGERTGIPLLIGLKLNDAVSRIIEASL
                     NVGRIRYDETIITHNDSLDARVYSQYPAYMENATLGFGTRVDIWLTLNQARIPKIERA
                     DSVNTMPAKHADEIIE"
     gene            complement(23857..24981)
                     /locus_tag="PK049_03250"
     CDS             complement(23857..24981)
                     /locus_tag="PK049_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HOK60605.1"
                     /translation="MEPIVKTIAPNINDEFLKHITSKVMDGQRIQPNEAVYLYEKADL
                     ALLSQLANITSSRINGDYVLFNKNFHIEPTNLCVFNCKFCSYHKGAGDLESWVYNLDD
                     IKKIAHNYQNSDVTEVHIVGGVHPKWDLDYYGKMIKTVKEILPNIHVKAFSAIELDYV
                     IRKSKLSYAEGLRKLKEYGLDSIPGGGAEIFDPAVRAKICPDKASGEVWLAIHKAAHQ
                     NGIQSNATMLYGHIESYKHRVQHMEAIRNLQDETHGFNCFIPLKFRSANNPMGYLGEV
                     NLTEDLRNFAVSRIFMDNIKHLKAYWPMLGIQNTRLAIHFGADDIDGTIDDTTKIYSM
                     AGAEEQKPVMTVNRLTQLVREAGKIPAERDSLYNIIKVYS"
     gene            complement(25067..25222)
                     /gene="rpmH"
                     /locus_tag="PK049_03255"
     CDS             complement(25067..25222)
                     /gene="rpmH"
                     /locus_tag="PK049_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005840676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOK60606.1"
                     /translation="MKRTFQPSIRKKRNKHGFRERMSTHNGRRVLAARRAKGRKRLTV
                     SDEIFRK"
     gene            complement(25581..26201)
                     /gene="rsmG"
                     /locus_tag="PK049_03260"
     CDS             complement(25581..26201)
                     /gene="rsmG"
                     /locus_tag="PK049_03260"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOK60607.1"
                     /translation="MEQIAQYFPELTQAQRDQFAQLEPLYRLWNSQINVISRKDIDNL
                     MIHHVLHSLAIAKVVQFAKGTTIMDIGTGGGFPGIPLAILFPNCKFTLVDSIAKKTKV
                     TSEVAKAVGLHNVEVITARAEDLKQEWDFVVSRAVAPVPKLMAWVEKNIHPGGINNLP
                     NGLICLKGGDIKEELKPYHKWATKWNIDDFFEDDYFVEKYVIHIEV"
     gene            complement(26213..26830)
                     /locus_tag="PK049_03265"
     CDS             complement(26213..26830)
                     /locus_tag="PK049_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOK60608.1"
                     /translation="MVMIPENLSEKAKYDLVLVDKAIKGDQKAYAELMDRYRDAIYYM
                     LLKMVNNKSDAEDLTLEAFGKAFKNIHQYTPNFAFSTWLFKIASNNCIDFIRKRKANL
                     ISIDQGPEDAEGNSMSPSSVIPASTPDPEENLIKEQNIRLIQEVVSKLKPRYRKLIEL
                     RYFKEYSYEEIAEELDLPLGTVKAQLFRARELLSNILSNLPPHHK"
     gene            complement(26824..27987)
                     /locus_tag="PK049_03270"
     CDS             complement(26824..27987)
                     /locus_tag="PK049_03270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOK60609.1"
                     /translation="MKITTCLIMEIIYNLSIYQKVIGVVFLVFVLIQLYFYLVFYLRV
                     IRKKFNDLPENDEQYPPLSVVICARNEEENLKHFLPKVLEQDYPDYEVVVVNDCSSDN
                     SDLVLESLQKKYPRLRVTTIKEDEKFTHAKKLALTVGIKAAKNEWLVLTDADCFPKSN
                     KWLRYMASNFTNGTEVVLGYGGYLAERGLLNKLVRLDTLFIALNYFGWALARRPYMGV
                     GRNLAYKKSLFFKNKGFANHAGIDSGDDDLFVMEVATPSNTAVEYRPEAHTLSVPRKT
                     YSTWLRQKRRHLSTAKFYSSNLKTRLGLEPLSRILVYILFALTLIFKILPLISTALLL
                     LVIIVKHTIAAIAAKRLEEKKLMHIAFWWDLFSLYFYAKLLIFNTLNRRQQKW"
     gene            28137..29270
                     /locus_tag="PK049_03275"
     CDS             28137..29270
                     /locus_tag="PK049_03275"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOK60610.1"
                     /translation="MSFSRFNLNPAVLEGIEAMGYTKPTPIQEQAIPVILEGRDLLAC
                     AQTGTGKTAAFMLPILSLLSAEEHDASSVNTLVIVPTRELAMQIDQQVEGFSYFAPVS
                     SIAVYGGNDGSNWDRQRQALEHGAEIIVATPGRLMQHIQMGYVNLKGVQHLVLDEADR
                     MLDMGFYDDIMAIIAYLPKQRQTLMFSATMPPNIRKLAKSILVEPVEINLAVSKPAEN
                     ILQAVYHVGASQKLSLLASLLSKKEVVQSAIVFASTKAEVKSIESKLHKEGLSVAAMH
                     SDLEQKQREEVMRNFRNRKVQVLVATDIVARGIDIEDIDLVVNYDVPSDPEDYVHRIG
                     RTARAQSDGVAITFVSPDQRGKLHGIERFLGTKIFVINYIPNS"
     gene            29355..31922
                     /locus_tag="PK049_03280"
     CDS             29355..31922
                     /locus_tag="PK049_03280"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HOK60611.1"
                     /translation="MKGRVADDTGRPIPYASIYSKELRQGTSSNSDGDYMLRVPSGEY
                     SFVFRGLGYETVERRIKVDVGEVTLNITMPVKPFLIAPVTVGGKGEDIAYDVVRKAIA
                     MAPYYRNLVSEYEAEVYLKGTFKVDKLSWLVKRSIKDSPNPPKEGSLYLQESYNNVRF
                     TAPEKFDQRVKMIRSNFPGELNSTDNILGFVSANLYQPKVGEIILPLAPYAFNHYLFK
                     YEGYAFQDNRALVKVKVIPKRKSRQLVEGYMYIAEDYWNLHEIDFTVQSLAGPFRIKQ
                     TFGEVERNAWLPISQYFDIDANFLGNVGRIQYVTAIKYLNVKLNSKVKPPVKIDIKEA
                     ETKHIAEDTQVKRNTSPRKQREDARMEQLLAKDNLTNREMYELQKLMQKQVRQSDSLP
                     NKLEVPNPLTITVDSVATKPDTMAWQQVRPVALNAEEFKVNREIDSKLQMSPDSVSAD
                     STGKANVLKTLYMGHTWYNANKKRTFLFSGFAGPNEFRFNTVDGFVVGASGSYRKQYS
                     RNGIYIKQSAYYATARKTLMGETDFRLSYANMNRGLASIRMGWISYDFNGDAGISPLT
                     NTSASLWFGRNYMKLYENRYVSLSNRIDPLNGFEVYTSVSFARRVELQNNTDFIVYQQ
                     NLRWYTPNVPANEDATPQNLSTHSSLEGSIRVRYTPFYHYRIRNGRKQMLYSRWPTIT
                     FQVHKALPNLLNSNSDYLRWEAMVNQIIRTGPSNRISYKFIYGDFASKGSLYFVDFKH
                     FNTMRVSVVIPSFSEGYQNISYYRRSTSECYAQAFLSYQSPYLALKYLPFLSNRIWLE
                     NLQLTFLKTAGYKPYYEIGYFLSQIGVVGGIGVFGSFEGKQFHSLSVKVSLIIPGFE"
     gene            32088..32663
                     /locus_tag="PK049_03285"
     CDS             32088..32663
                     /locus_tag="PK049_03285"
                     /inference="COORDINATES: protein motif:HMM:NF027619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOK60612.1"
                     /translation="MRKKDEAKPEVIFEASLDVLINHGFVNFSMGRVAKQAKIAVGTI
                     YLYFKHKDDLLNQLYFKLYKESVDRFLEHYNPNNSFRESLKLVWVNYLKHRINHYKES
                     HFLEQYYKSSYINDSHKQMAEQMKKPIHDIIQKGKELGLVRSDLDNEMIFLGMLGFLR
                     ELADEHVGNVYILDEDRINKAFEMSWNMIKA"
     gene            32689..33495
                     /locus_tag="PK049_03290"
     CDS             32689..33495
                     /locus_tag="PK049_03290"
                     /inference="COORDINATES: protein motif:HMM:NF039830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5692 family protein"
                     /protein_id="HOK60613.1"
                     /translation="MAIPFWQYLVSMPLYIVLLMLAVEFMRTNYRFAAVFWVLSLFTF
                     PLWFNNLEGWFRWAKTLSVLLPTAFVVGCARIAQYEKRDGWWTIFRKDWVLWFLYAIL
                     GLNILEASLKDFEMGNWFNGLSGLILIVTIPLVKSGKGKRYGWKISEEKPGDLIAYTD
                     AIWNFLYTSWNIAFVYAENPGYAASSLCILLAAELYPVIKRRPELYVQARVYTLAIHI
                     LIRATYDIFTPIMDSSAFASQNVVFWWGIVNFSLHLPYLIWYFFLKKTEK"
     gene            complement(33729..34421)
                     /gene="rsmI"
                     /locus_tag="PK049_03295"
     CDS             complement(33729..34421)
                     /gene="rsmI"
                     /locus_tag="PK049_03295"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOK60614.1"
                     /translation="MSKLFIVPTPIGNLADITLRALDVLKTVNLILAEDTRTSSNLLK
                     HYGIATPLKSHHKFNEHQTVDYLASRIEAGESVALISDAGTPGISDPGFLLVRTCVER
                     GIEVECLPGATALIPALVQSGFPCDRFIFEGFLPQKKGRNKRLEALKDEERTIILYES
                     PFRIVKLLEQMLPIFGADRQVCIARELTKLHEENFRGSLAQAKEHFSSKSPKGEFVVV
                     VKGKTNNEKEED"
     gene            complement(34434..35153)
                     /locus_tag="PK049_03300"
     CDS             complement(34434..35153)
                     /locus_tag="PK049_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60615.1"
                     /translation="MQYLKNARNQLVEAVALIDKALSAGKLPSIERDILLAKLASIYE
                     DLLLDRQQVASRTHEKPQVVPSGKMVEPHEKTIASIPETKKTTVVEESAEPSSIPTAK
                     QEPNPVKADISPSPKPTPAPTQQSHEESSAILADKYQGKRKFRNEVIAEQHQKVDMQS
                     KLQNKPITDLAKAIGINDKFLFIKELFGGDADLYNQTIKHLNQLTDLNEAIIYLQENF
                     SWDPTNEVTTLFVDLVRRKLS"
     gene            35240..35827
                     /locus_tag="PK049_03305"
     CDS             35240..35827
                     /locus_tag="PK049_03305"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005828444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOK60616.1"
                     /translation="MPFLENPVSGKRKGYIEVIAGSMFSGKTEELIRRLKRAKYAKQR
                     VEIFKPMIDTRYSEQEVVSHDANSILSTPVSSSGNILILAQNVDVVGIDEAQFFDMNL
                     PNVCSQLADMGIRVIVAGLDMDFRGKPFGPMPNLLAIAEYVTKVHAICVKCGDLAQYS
                     HRKTDADKLVLLGETDIYEPLCRLCYNEVTRKNRK"
     gene            35860..36330
                     /locus_tag="PK049_03310"
     CDS             35860..36330
                     /locus_tag="PK049_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60617.1"
                     /translation="MQKINRLGKLLGPVGSIAGYFIALVGIIQLFSSFLAIVLIIIGL
                     FIGFTSFAAQVDFGNKRFRNALLLFGIIPIGKWQSVDSSMGLSVKRLNRAWRTYSSSN
                     RSFDIADSDYRIVIVDKQGNPLADIQRVQSPEKANEALVYYSEQLGLNILRPSR"
     gene            36354..36776
                     /locus_tag="PK049_03315"
     CDS             36354..36776
                     /locus_tag="PK049_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HOK60618.1"
                     /translation="MKTQVDISHRISPKLFWFVTLMIITLPITTQYRLLIFGEYTTGE
                     VVGVEYEKTLGISAFGPDKYPVIVFTTLEGKSVQFYGPENFEYPKGTMLKVVYSPNNP
                     TDCMVFSFGALYTDRKAIIPGILFLLWMAFYLAFRNKK"
     gene            36826..39294
                     /locus_tag="PK049_03320"
     CDS             36826..39294
                     /locus_tag="PK049_03320"
                     /inference="COORDINATES: protein motif:HMM:NF008897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine
                     ligase/alanine racemase"
                     /protein_id="HOK60619.1"
                     /translation="MINLTAIQLTEIVQGQVIGDSGFTIRNVITDSRVPVSGNGSMFI
                     ALVGPNHNGHEFIGELYNIRNIKAYLVEKGEVDYTNYPNATFVEVDDTLQALQKLAAY
                     HRNRFVYPVIGITGSNGKTIVKEWLNQLLFSEYQIVRSPRSYNSQVGVPLSVLQMDNH
                     FDLAIFEAGISKPGEMVKLQPIIDPTIGIFTNIGMPHQENFDDIDEKVNEKLKLFREV
                     KTLIYCKDHAVIDAVVQDNTLLADNQLLTWGQNKESKVQIMSVKREAGKTLVHVRFNN
                     HEHTITIPFTDAASFENAMHCVATMLLLGYSLQDIDTRLQRLQPVAMRLELKEGINDC
                     SVINDSYNSDLGSLAIAIDFLMQQKQHERHVVILSDILESGYAPEQLYSKVAQMLEDK
                     GVDLLIGIGPEISRYDYAFEIEAEFYSSTHEFLLDLKRKEIRDAAILVKGSRKFHFEQ
                     ISAALERKVHRTTLEINLNAIAHNLNYYRSLLNPGVKVMAMVKAFSYGSGSYEIANLL
                     QFQRVDYLGVAYTDEGVSLREAGISLPIVVLNPSFGTYELMVEFNLEPEIYNITGLKE
                     FINVLNRVGVSSYGIHIKIDSGMHRLGFTPDQLDELIAILKSTNSVRVKSIFSHLAAS
                     DEPHHDQFTIHQIETFHKACNVLSDVLGYQPIRHILNTGGIERYPEAQFEMVRLGIGL
                     YGISPTHGDKLMPISTLRSKVIQVKQIKPGDTVGYSRKGIVDTPTTVVTIPVGYADGL
                     SRMLGNGNYSMMLNGTLVPTIGNISMDTCTLNANGVEVKEGDEVIVFGNKPTITDMAK
                     AMETIPYEVLTSISQRVKRVYFQE"
     gene            complement(39296..40003)
                     /locus_tag="PK049_03325"
     CDS             complement(39296..40003)
                     /locus_tag="PK049_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004342720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOK60620.1"
                     /translation="MSRNSYNIDIESVFEGVCGLFPNLKPEEREIMRNNITCQYFRKG
                     EIIYKEGDTPQGLVCLSKGKVKLYKEGVGGRDQIVRMAKPIGFIGYRALFAEENYSAT
                     AEALEDSVVCIIEKNAFMGILRNNAELTLIILKSVATELGFSTNRTVTLTQKHIRGRL
                     AESLIFLRDTYGLEDDGKTIKVYLSREDLANLSNMTTSNAIRTLSGFAEEGVIELDGR
                     RIKILDHHRLERISDLG"
     gene            complement(40098..41765)
                     /locus_tag="PK049_03330"
     CDS             complement(40098..41765)
                     /locus_tag="PK049_03330"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HOK60621.1"
                     /translation="MKVALAQLNYTIGHFDSNADKIIASINRAKADGARLVVFSELSV
                     CGYPPHDLLTHKHFVDKSIATVNQIAQQCNGIYAIVGVPSINTDTTGKLLFNSAYLLG
                     EGKVLDVYHKTLLPDYDIFDEYRYFEPNPGNYRVAEIDGIKIAITICEDIWDDQPTTT
                     AFSRSKLYRDAPMNHLVKLNPDMVINISASPFSYNVEERRLQVFQSNVARYSKPVIYV
                     NQVGGNTDLLFDGGSMVVNAGGDIIEKLKSFEEDFRVIDTNVLERNQSIPPEISATSD
                     QRIERIHNALVMGIRDYFGKLGFTKATLGLSGGIDSAVVLALAAQALDVNNIRVLLMP
                     SQYSSQHSIDDAVELAQKLGVQYDIVSIQPIFESFKNQLSSIFEGRPEDITEENIQAR
                     VRGTLLMAISNKFGHLLLNTSNKSEAAVGYGTLYGDMCGALSVLGDVYKTDVYRLANY
                     INRNDEIIPINTIQKPPSAELRPDQKDSDSLPPYEILDPILFAYIEQGLSAEEIVGLG
                     FPSDTVNRTLRLVNMNEYKRYQSPPILRVSSKAFGYGRKMPLVTKWS"
     gene            complement(41782..42447)
                     /locus_tag="PK049_03335"
     CDS             complement(41782..42447)
                     /locus_tag="PK049_03335"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOK60622.1"
                     /translation="MKLNSLTLLIGLILLGNQLANAQQYSFGVFADPQLSWFNSDTKR
                     YDPNGPVVGLNIGFTADKFFAKRYAFSSGLSVNTLGGNIRLMQSTYTLKTIDGTYNIA
                     IGDNIKFKSQYLTLPIGFKFRTNQIGYTTYFANVGVSGSLRIRSYAWNETNHVDRETT
                     KTDMAWGFASYQIGIGGEYSLGGESAILFGITWCDGLTRIIELPNSTITSQSLSLKVG
                     IIF"
     gene            42612..43433
                     /gene="ispE"
                     /locus_tag="PK049_03340"
     CDS             42612..43433
                     /gene="ispE"
                     /locus_tag="PK049_03340"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOK60623.1"
                     /translation="MILFPNAKVNLGLNIVRKRPDGYHDIETLFYPVKGLCDILEVVA
                     TDGEGNTIEFTQTGIGIDCDTENNLCVKAYRLLAQRYGMPHVRMHLHKLIPIGAGLGG
                     GSADAAFALRALNTFLDKPLSHENLLSMTLELGSDCPFFVHNTPAIGRGRGEQLEPIS
                     INLTGYWILLVNPGIHVSTREAYEGSNPKPWKNPIGNIVNLPVSDWPNKLVNDFEKTV
                     FPKYPLIERVKEELYSMGAVYASMSGSGSTVFGIFKSKPVITDAFKGYFTHTSEM"
     gene            complement(43563..44063)
                     /locus_tag="PK049_03345"
     CDS             complement(43563..44063)
                     /locus_tag="PK049_03345"
                     /inference="COORDINATES: protein motif:HMM:NF025725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4411 family protein"
                     /protein_id="HOK60624.1"
                     /translation="MPVYVLDSNFFIQAHRFHYPIDVAAGFWNKVRQLAEEGRVISID
                     KVKKELYDRNDALEEWCRNNLPEDFFKDTSVVMTAYAQVTAWAMSRSGHYLPNALNEF
                     LNADEADAFLVAYCLADNANRFVVTQEVSEPNRQNKVKIPDACIALNVSYVNTIEMFR
                     QLGETF"
     gene            complement(44069..45199)
                     /locus_tag="PK049_03350"
     CDS             complement(44069..45199)
                     /locus_tag="PK049_03350"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HOK60625.1"
                     /translation="MANEINVNTNMLTWAITRAGYDVPTFAVKFPKILEWLEGQKKPT
                     VKQLEEFSKKVYLPFGYLFLPQPPQEKLPIPYFRTNGNQADRISINVFDTILLLQQRQ
                     DWLRNYLKDNDFHPLPFVGKFRNSNNVNAIVADIRQTLELTENWASQFRTWQDAQDHL
                     VLHIEDKGIITIFNGIVENNTHRPIPVDECRGFVLVDEYAPFMFVNNSDFKSAQMFTI
                     VHELAHIWTGHSAGFDFRRLQPANDPIEILCDKVAAEFLVPAQEFDEVWNHKPNNFAY
                     ASRYFKVSEIVIARRALDTGKISRPQFFDFYEEYSNREFAKKESQGSGGDFYATTKKR
                     ISITFASHVNQAVKSGKLLYRDAYKLTGLKGDTFENFFSKHL"
BASE COUNT        12061 a         9601 c        10316 g        13189 t
ORIGIN      
        1 ttgataatct tccctaagca ccctaaactc agttcttcgg ttgatttgat gaactgtttc
       61 tttttgatcc tcactggtta aggagtcgat aaatttctta tcaagaacag ttcctggtgt
      121 taggaatggg tattgggcag caaggttatt gtcaacaacc tttggttggg tttgggcgta
      181 gcctttggct ttcaacctat cggttgctat gccgttttca atcaaatagt ttactaccgc
      241 ctgagcacgc ttttgcgata gctcatagtt atactcctga gagcccctac tgtcggtatg
      301 cgatgccagc tctatggtta tgttggggtt gtcgttcagt atctggataa ggttatcaag
      361 cgcttctttt gactcgggac gaagttccca cttgtcgtaa tcgtagaaaa tgttgggtat
      421 ttcaattggc ttagcggttg aggttaaggc tattgaaacg ttaaaatcct ggctcttggt
      481 taacccttta gtggtttcct tcgccttatt gttcaggtat cccttctttg aagcaacaat
      541 tacgtaatcg gtgttgggga caaggttaaa cctgaatgaa ccatcgctct gggtctgcat
      601 ggttgcaatg gtgccatcgc tgccaaccag tttaacggta gcatcggcga ttgtagttct
      661 tgttttttca tccattacct tgcctatcat gttaaagttt ataggcggga ggtagaacat
      721 gtagatatcg tcggctccac ggccaccatt acgccgtgag gagaagaaac ccgcttcacg
      781 atccttttcg aaaataatac cgaagtcatc ggcggttgaa tttaccgggt agcgcatgtt
      841 ctcaataacc cattggcctt catcgttttt cttagcctta aatatgtcga ggccacccat
      901 gccaggatgt ccgtcggaag agaagtaaag ggtgccatcg ggatgaatgt atggaaacat
      961 ctcgtttccg ggagtgttga tttgtgggcc aaggttaata ggcgcgctcc actcttcgga
     1021 acccgcttcg cgggtaattt tccaaatatc gagtcccccg tttcctccgg gaatgttact
     1081 tacaaagtaa agttcagttc catcggccga aatggctgga tgggcaacaa tcatactatc
     1141 ggcagccagt tccaactctt taggggtaag ccaaccttgg tcgctttgct tggcgtaaag
     1201 tatctggcat ccaagcttgt tacgcttgct aaccttacaa cgggtaaaaa acaagttgtt
     1261 gccatcggga gttaatgagg gggtgccctc gtcgaattgc gagtttatgg tttcatccag
     1321 aggttttggg gcactccaaa cccctttatt gtcgagctca gtgataaaaa tgtcggaaaa
     1381 attctcgccg gttacggcgc ttgtcttgtt tccgatagta ccgttgcggg agctggtaag
     1441 gtatagaata ttgtagtcgg ttgaagcgta tgctggtgag aaatcgctaa actttgagtt
     1501 aatggcgcgc atgttggtta tttcataacc agccggtgtg gcaatccact gcttggcaag
     1561 ttcacacgat tgtattccga cctcggcaag gggatcgtta gggacaagaa ctttgtactt
     1621 ttggtactgt tcaatggcaa ggtcgtactt ttcattcatc ttcagcattt cagcgtagta
     1681 gtagtaaacc ttagggtttg ggcattccct tcgaatggct ttttcgtacc actgctcggc
     1741 ctggcggggt tctcctattt gtctaaaaca gtttcctatt ttaaaaaggt agagggaaag
     1801 ttccttttta tctttaatct tgtcgatatc gtcgcggtaa agggcaatcg catcaaagta
     1861 tgcaccggca ttgtatagcg catcggcttt ctctaacttt ttagtgggtt gagccgaaat
     1921 gtttcctgag gttaggtaaa ctaatcccaa tgcaataaga agtagtcgct tcattgtgct
     1981 ttaaaggttt tagtcacaaa attatggtaa atataagtaa atgccaatta agtagtaacg
     2041 caagggctat ttttttatta taacttattc acaatggttt ttcggcttag tagggtagat
     2101 gcttactttg ctcgcccatt taaaaggatg accttattta atgttgatta gggttagtat
     2161 ccaaatacat taatcattca atcacaagag tgtcaaaact acatgtgcaa ttaagtacgc
     2221 cccgctttaa agagcggggc gtatggaaaa caaaaaagtt ttagcagttg aatgctatta
     2281 ccgaatgaat agcatctccc tatacttggg caaaggccac atctcatcgt caacaatcat
     2341 ttccagctta tcgatatgcg tgcgaatgct ttcaatatag ggtttaacgt ttttcccgta
     2401 caagtgggct ttttcaacaa tatcttcaac ctggttggct tcctttcggc gttcaatcat
     2461 ctcgttaacc tgatgactta tggcatggat atgttcagaa atttccagga ttaaagcatg
     2521 ctgatgatcg gccagttttt cccattcatt tgggaaaagc tcctttaatc ccttaacgtt
     2581 gctaattaag gtgttttggt atgcaatggc agtggggata atatggttga tagctaaatc
     2641 gccaatcacg cgggcttcaa tttgtacctt ttttacaaag atctcgttcc ttatttcgta
     2701 ccgtgcttca agctcgcgtt ttgttagtat tccgttaccg gtgaggagtt ttacagagga
     2761 gtcgagcagg aaagtcttga atgcctccag tgggttgctg atgttaggaa gccctctgcg
     2821 tgctgcctcg tcaacccatt cctggctgta accgttgccc tcaaagcgaa tcttttttga
     2881 ctcaataatc accttgcgca ggcactggat tatggcttcg tcctttttaa ttcctttggc
     2941 aattacctta tcaacctcat ctttgaactt catgagctga ttggccacgg ctgtgttaag
     3001 cacaatcatt ggagatgcgc aactgcccga ggagcctaca gcgcgaaact cgaatcggtt
     3061 tccggtaaag gcaaatggcg atgtacggtt gcggtcggta ttgtctaaaa ggatttccgg
     3121 gattttacca atgatttcca gtttgagttc ggtcttttcg tcgggagtca ttttttggtc
     3181 tgtcaccttt tgctccagct cgtcgagcac ggcacttagg gttttgccga tgaacactga
     3241 catgatggct ggcggtgcct cgtgcgcacc aagtcggtgc gagtttggtt ccgacgctat
     3301 actacccaaa agtaatgcgg agtggtcatg tacagcttta atggtattga ttaggaaggt
     3361 caggaattgt aggttgttct ttggtgtttt gcccggtgaa agcaggttag tcccagtgtc
     3421 ggtcatcatt gaccagttgt tatgcttgcc tgaaccattt atccccttga agggtttctc
     3481 gtgaaataga acacggaagc cgtgctgacg agcaatacga tccatcagcg acatgataag
     3541 ctggttgtga tcgacagcca ggttggcctc ctcaaaaatg ggtgcgcact caaactggtt
     3601 tggcgccacc tcgttatggc gggttttaat aggtatgcca agcttaagag cttcaacctc
     3661 aaaatgacgc ataaaggcgg aaacccttgc aggaattgaa ccaaagtaac gatcctcaag
     3721 ctgttgatcc ttggccgcag cgtgtcccat aagcgttctg ccacacaatg caagatcggg
     3781 ccgggcgtta tacagcgatt catcaatcag aaaatactcc tgctcccagc caagcgtggc
     3841 ataaacttta cgggcatcct tatcaaagta ctccagcacc gaaagggctg ccttatcgag
     3901 tagtgcaagc gatttgagca atggtgtttt aaagtcgagc gcctccccgg tatacgaaac
     3961 aaacacagtg ggaatacata gggtattctc tagtataaag gctggagagg ttggatccca
     4021 agcggtgtat ccacgggcct caaaagtatt acggatgccc ccgcttggaa aacttgaagc
     4081 atcgggttct tgttgaacaa gcatatcgcc tttaaaaacc tcaatgggtt gacctttgat
     4141 atcaattgag agaaatgcgt cgtgcttttc ggcggtggaa ccgtttagcg gatggaacca
     4201 gtgtgtgtag tgagtagccc cacgctcaat agcccaggcc ttcatggccg acgcaacctg
     4261 gccggcaatc ttacggtcaa tctttttacc ctcatcaatg gaggtgataa tactctcata
     4321 agcttcttcc gataggtact tcttcatttt aggcctatcg aatacattga tggcaaagat
     4381 atcgacaagc gattctgctt tgccctcgcc gttcatgtca accgggatgc gattagttgc
     4441 ttcaccgaat gctctaaatc gtagttttgg catagtgtgg tatgtttttc aagggcaaat
     4501 ataagaaaaa aaatcaaaat aaatagttta gatatataaa aaataggggt atgtatgcag
     4561 aaaaaataaa aatatttaat atgtataaaa ataaacaggg ggtgtgatgt aaaaaataag
     4621 tattttgtta aatggttgta ggtttaaaat tcttatcagg aatcgtccct tgttttgata
     4681 ttagctttgt atttgtttct tggtagcggg ttgcttgcct tagtggttta ttgtgaacac
     4741 atttatccat tatgtatcac cttgggttgg tttttgaaca aagtgttgcc gttttgcgtt
     4801 ggtttttgta aattgcaaac atggaaagcg ataacagaaa atattccaac gacgaaatca
     4861 ctgtgttctg gcgaccaggg gagtgtgttc atgcttccat ttgctacact aagctgctct
     4921 cagtgtttaa cccccgtaaa cgaccttggg ttaacatgaa cggtgccagc acagagcgaa
     4981 ttattgagat tgtgaatgag tgcccaacaa acgcacttac ctttatgtgg aacgatcctg
     5041 aaaaaaataa gcaagagcgt tcgcacaaat gtgttcggga atttactccc gaggaggttg
     5101 aggcgtttga ggttacgccg gtaaagattc aggtaatgcc aaatggccca ttgttggttt
     5161 caggcgattt tcagattatt gatgccgatg gcaaggaggt taagtcaatg aaaatggttt
     5221 ccatttgccg ctgcggtcat tcaaacagcc agccattttg cgatggcaca catttcaaaa
     5281 aaggattccg tgatagagag tagcaaaatg aaccagccaa aaaaaattgc agcaaagctg
     5341 catctaagca aaggaggccc aattagggtt gaggggcgct tctttattgt taacctcgat
     5401 ggtgaaagtc ttatacccga taactccaag gaagttttcc tatgcacctg tggggcatcg
     5461 cgcaataaac ccttttgcga tggtagccac aagggaggaa agtccaatgg atagagttat
     5521 tttgtgccta gggtacctgc ccgtatggca ataagctaga ataatcaaaa taattatcat
     5581 ttaacttaat tagccagttg tgcctgaaca cttttgctat ctttgcatgg cataagtttt
     5641 aacgggcact aacttggaat tacataaagc aagaaaagcg aaaagccctg tgttaagggc
     5701 tttgtttgtg atattcggca tagtatcatt tgggttggga gtgttgggca tttttttacc
     5761 attactaccc acaacaccct ttatgctgct tagcgcattt ctgttcatcc ggagctcggg
     5821 gaggctttac cggtggttaa taaaccatcg gcttttcggg aaatacatac acgattacat
     5881 tgaacgccgg tcaattcccc gacgagtgaa atggtatact ttaggattgc tctgggcatc
     5941 tatacttaca agtgttgcaa cacttgatgt tggcaacgtc atccggttaa tactactgtt
     6001 aattgcggtt ggtgttaccg cccatgtggt aaagttacga aacagctagc cttcgctact
     6061 ccctgctacg attaagggca tcgtagatga ccagtgtgaa gaattggcgc atgttcccac
     6121 ccatggcctt aacctgctgg gggataatac ttgcctcgct catgccgggc acggtattta
     6181 tctcaaggaa atagattttg tcattattaa caatataatc cacacgaacc acaccgtggc
     6241 attgcaatcg atcgtatatc tttgatgtga attgttgtat ggcactggtt aacgattctg
     6301 ggattggggc aggggtaatc tccttcgact tgtttcggta ttttgcctcg taatcgaaaa
     6361 actcggtttc agggactatc tcggttacgg gcataatcat ttctccattc tggtatttga
     6421 aaacaccgca ggtgaattct cggccgggta tgaactcctc cagtataatg gttttatctt
     6481 cggcaaatgc tttttcaatg gcgggcatta agtcagaggc ctgcttaacc ttgcttactc
     6541 cgtagctgct gccgctctca ttgggtttga caaaaatggg taatcccaaa gtgtccagaa
     6601 ttgcttgcgc atctactttt tgaccgcgcc tgagcagcaa tccctttgcg aggttaactc
     6661 ctgtttcgcg aactaactcc ttgcagtaat acttgttaaa ggtaacggaa gagttcataa
     6721 ctcctcctgt ggtgtaggga atttgaagca tctcaaagta tgcctgaagc agtccgttct
     6781 cgccgggagt gccatgtatg gctatcaggg cgcagtggaa cgatagtttt tgtccatcaa
     6841 tatcaacggt gaaatcgttt ttattgacag gtatttgacc tttctggggg tgttcaagtg
     6901 tccaggtagt accttttacg agtattttgt atgcattgaa ttgttcgggg tcgagccaac
     6961 cggcaatctg ggtggcgcta cgcatggata tgttggcctc cgatgagtta cctccagcca
     7021 ttatggcgat gttgagcttt gtgttcatag tgttttaaat tcattgcaaa gctgcaagaa
     7081 ttttggcaaa aataaaagag tcgcaggcgg aaactgcgac tctttttgaa gtaatctttt
     7141 gtttttatcg ggcatcggag aagtaacgca tgaactgtgc tctctcgtat gccagcggtt
     7201 ttatgctatc ggcttggcgt agcttgccag taatggaatt aatggattca aaatcattgg
     7261 catctagcca tttactggtt tcctcaatcc atggttcaag tatctcgggg cccgatttgt
     7321 acatagccga agccacctga accgcattgg cccccaagag gatattcttg accgctgttt
     7381 gccagtcgtg tatgccggtt gaggctgcca ggttgcatgc aactcgccct gaaagaatgg
     7441 aaagccatcg gcgaacctct gcattttcat cgggatggct gagtatgctg cctgaggtaa
     7501 ccttaagggt gttcaagtct acatcgggct ggtaaaaccg gttgaaaagg acaagtgcct
     7561 taattccggt ccgggatagt tccacggcaa agtttgccat gcctgaaaag tagtgatgta
     7621 ttttcatggc aacgggaacc gatagtgttt gtgctacctt gcgggcaaca tcgatgtaaa
     7681 ttttctcaat ctcatcgcct ttcatcttaa catccgaagg cattataaac atgttgagtt
     7741 caatggcatc ggcaccggcg gcttgaatat cgcctgcaaa gtcaatccag ctatcggcgc
     7801 tactacagtt gatgcttgca attaccggta tacttagctt ttgcttcatg ccatcaatta
     7861 gttcaacata gttgctaagg gcatgcttgc gggtgtagta tgcaatgtag tcctcggctt
     7921 cgctgtagga gttgaacatg ttgttcatgc gttgggcatc aacatccatc atgatttgct
     7981 cttcaaagag cgatttaacc acaatggcgg caaatccatg tttctggagc agggctgctt
     8041 tttcaaccga gtcggtaagt ccagagctcg atgcaatgta tgggttcttc agcttgaagc
     8101 ccatgtatgt ggttgatagg tctgccatat cgttttaagt ttaagttgat tgacttcttt
     8161 tactttttgc cacccaggaa cctatcgatt ccaatggctg ctttatggcc gtttgcaata
     8221 ccatgtataa catcggggcc ctgaatgata tcgccaccca caaagagcca ttcgacgctt
     8281 gactggaagt agtcattaac tactattcgt ccgcggctgt cgagtttaag attatccttg
     8341 atgctctcgg gtaggtagct taaatccatt ccctgtccaa tggactcaac caccatttcg
     8401 ccggcaaaga aatcggtttg ggtgtcatca aacttggggt tgaaacggcg attctcatcg
     8461 aacactgaaa cgcacttaac tacgtgtaag ccttttattt tgttgttttc aatttcgatt
     8521 ttaacgggtc cccatccggg aatgattgtg gcaccttcct cgcgggcctc aacaacttcc
     8581 tcgcggtcag ctggcatctc attttccgat tccagcgagg tggttatgat attcaccttg
     8641 ccatacctct ggttctggat tcgggcaagt gttcgggtga tatccatggc tacgttaccg
     8701 ccaccaataa ccacaatact cggggcagca ggaatttcct tgcccatggt tacatcgcgg
     8761 agcaggtcaa cagcctggta aacatgggga tggtcagtac cgggaatgcg ggtgctacga
     8821 ccaaggtgta agcctgttcc ggtaaacaca gcgtcgaact ggttgtgcag ttcctccaga
     8881 gtgatatcct tacccacggt agtattgcac cggatttcaa cgcccagcga tttgatataa
     8941 tcaatgtcct tgtcaagggc atcgtagggt agacggtact caggaattcc atagcgcatc
     9001 attccgccgg gcaacggtaa tttctcgaaa atggtgcact catagcccat tagggcaagg
     9061 taatgggcag ccgataggcc ggcaggtccg gctccaataa tggctatccg cttcccattc
     9121 ttgctgatgt tctcggtttc cagaatttgt ttgtaattct ccgcgggttg ctggtcggca
     9181 atgtagcgtt taagccagcg tatggagagc gggtcgccct gatgccccac ggagcaaaca
     9241 gtttcgcact tgtgtgtaca aatgcggccg caaacctctg gcagcgggtt ggtgtcgtag
     9301 aggatgcgta acccttcgtt caggtcgtcg ttacgtattg ctttaatgta ttcaggaatt
     9361 cccatgtgtg ctgggcaggt tgcaacgcaa attccacatt ctacgcagcg gtcagcttcc
     9421 tttatggcct gttccctgga atatccgcga acaatttcaa tgaacgactt gtcgcgttcc
     9481 tcgggtttaa gttcgggcat atgaacgcgc tccttgctgt agagctcgta gtttggccca
     9541 ggttttttgt agccattggg ttcgtcgtcc cattctttta tgtcgacccc gggaataaac
     9601 cggaatacat ctggatcgga atcgacccac ttgaagtgat tcgacatgga cagtgagcct
     9661 gtggtgcaaa catcaacgca aagggcgcac cagcaacagc ggccatagtc gatacggggt
     9721 cttaacccgc tatcgccatt ttttgtctca atgccctcaa ccggaaccat atcgattgcc
     9781 gcattctggc atattgactc gcatgttccg caccctatgc aggtatcaac atcgttcttg
     9841 tggaaacccc tgtatctggg tgcagcctct ctcttgaggg gttccttaat ggttacaggc
     9901 tctttaacca catttttcca tgcggtaaag gggcttatga tatcttttat tcgtgccata
     9961 ctttacttac gtttcaataa attacctttc aatttcaggt ggataggtgt gcaacgaggt
    10021 cataaggcca gccacatcgg caaggttggt attgattatc attttttcaa gcaatgcaac
    10081 ggcatgggtg tagcttggcc cgcgaacatt aatacggcgg gggtactcgc tgccatcgga
    10141 aacaatgtag taggcaaact cgccgcgtgt acattcggaa cggataaagg tttcacctgc
    10201 tggtatttgc cagtgaagca cattgggagt ggatgcctta aaatcgccat cttttggcat
    10261 tttggccagg atttggcgga tgaggtcaac cgaaagaagc aggtcgcgac ggcggacatc
    10321 ggctcgggta tagatatcgg aatccttccc cacaacgggt tcaaagtcga gctcggggta
    10381 tttcaggtaa gggtactttt tacgaacatc atcggggatg ccggccgcac gcgcgttagg
    10441 gccggtaatg ccaaactggt aaatccattt tgggtcgata tatcccatac caacagtgcg
    10501 tttcttgaaa acagcattgt tgaacatcac cctgtcaata tcaaccataa actcctcaat
    10561 tgttttgagg gtttcctcca tgcgtgcttc aaatccatcg ggcaaaagtc ctcgaactcc
    10621 tccgggtatg atatacatgt ggtatatacg tccaccggta agttcctcaa agcggtcgag
    10681 aaccaggtcg cggcagtagt tgctccattg tccaattatt cccattccca gcgagccggc
    10741 ttgtccgcct atccacatca ggtagctttg caggcgtgcc atttccaatg taagagttct
    10801 aagccattgg gctgtttcag gaatctcaat cccagccaac tcctcaaccc cagcagcaaa
    10861 acagtactcg ttgaagtcgg gttcaggaac ggtaattcgg caaacaatgg ggaaacactg
    10921 aatccatttt cggcgctcca tcagcttctc aaaaccgcgg tgtaggtatc ctacgtgggt
    10981 ttcgcaatcc ataatctcgt cgcctaaaag ggttagttca agcgacatgt tccctgttat
    11041 gcccgggtgt tgtgggccat gccatagctt cacatactta tgtgagctaa ggtcaatatc
    11101 taccttgccc tcgctgttaa gcggtgggta aaggcttcta tcctcgttta tcttgaacag
    11161 tttgctaaat ttcatcgggg taaagttttt tcttcatata ctcctttgga tcatgagttt
    11221 cccttcccgg acgagggaag taggtttcct cggcgtactt cttggtatca aagtcgcggc
    11281 ggtagggtgg gatgttgtcc caaccctcca gtataaaggg cttatcaacc cttgggctac
    11341 cgggaaagtc aatgccaaac atttcgcgga gctcacgctg gtaggttgca gcttgttccc
    11401 aaaggtgatg aatggtttcc atcgtgggat tatccctagg aataaaagtt ttaaccatta
    11461 catcaatcct tttgtttgga ttgttaagga tgtaggtgag ctggaattga tttttttcaa
    11521 tccaatccac ggcagtaagc tgaaccagat gaaggtagcc ttcaacctcc ttgaggtgag
    11581 taaccagtgt taccagttgt tcctttggaa tgctcatgtg gtagagccct ttacgctgtg
    11641 tccacacttc ggcattaggg aaaacggttt ttatgttatc aagtagtgtg ttcatggtcg
    11701 taacttttac cagttataat cgggtatatc ccaatcctta attatctttt tctgatttgc
    11761 cttgtaccaa tcaaagtttt tggcataaag gtttgcccct tcacccttgc cctggcgaat
    11821 aagctctttc agtttctcaa acccagcaag aatagcttcg gggcgtggca tgcagcctgc
    11881 aatgtaaaca tcaaccggta agtaatggtc gagtcttttg atggtattgt agctatccca
    11941 atacattccg ccgtttattg tgcaactacc tagagcaata acaaactttg gattttgcat
    12001 ctgctcgtag ctgcggataa cccttttcag ggttttaacg gaaagatatc ctgatataat
    12061 gaataccgac gattgacggg gtgtggggcg ccactgtatg ccaaaacggt acgcatcgaa
    12121 ccgtggggtc atgagcggac gcatctcaat ggcaccgcaa ccggtgccaa agcctaacac
    12181 ccataacgat tccgagcggg cccagttcaa aaatttttca aatatgggaa gtaccggttc
    12241 gtgaactttt ggacgagcgt cgcaaaagta gtcctgaagc ggatttatct ctattggttc
    12301 cccatcgggc gacattactg tccttttctc taaatcactt ttactcatgg ttatagtgat
    12361 attacttatt tcataatcag attaaaagaa tggctataat tccaaaaagt agtggaacgc
    12421 ctaagaacca gcgaacgctt tggtcaaccc tgaagcgggg aaaggcaacc ccaacaaaaa
    12481 ttgaccatag gtatatgaaa aaggttttaa tgaatagctc gggccaactg gttgcacctc
    12541 catacagtaa gttcatgaat aggattagtt ttgccacagg gaaaatagct cggttaagct
    12601 gcagcacacc tagaaatcca gaatggtact ctgtgggtgg gccaatagga atttcctgtg
    12661 gcgctaaaac aacatcaaag ggagcacgtc ccatggaacc taaaaaggtg agcattagag
    12721 cggcaccggc aaagggatta gtaaaaatgg tccagttcaa tataccgcct tgttgcgctg
    12781 caacaatatc gctaatattg agggtatggt actgcatggc tattgatatt actcccaggg
    12841 taagaggtag ctcaagtgca gtaacctgcg aaagacctct ggaaacccca attgccgaat
    12901 atggatgtcc accttcacct gcccctaagg ccatacccaa tacaccaaag aattgaaaga
    12961 aaagaacaag ggtaaggtcc ccagcaaacg atagattatc aaacataggg gaaccatata
    13021 ttacaggaag gaaaaaaaga agtccaattc ctccggtaag tctaaaaact gggcctaagt
    13081 agtacattac accatgtgta attttgctcc ttttggcatg attctttatt aagtcgaggt
    13141 atggttgata ccatcttata ccataacgtc tcccaactct tgctgctatt ttgggcataa
    13201 tgccctgaag aattagcccg taatttaaaa ctataaaaag ggttagcagc aagtatagta
    13261 tttgtttaat tccaaattcc atttagaatc ctccgttaat taagatgtaa actattacag
    13321 taaaccctag gatgtgaact aggtaagttt gtccattacc attgtagatg ctcctgattt
    13381 tatcggctat agcatgtgcg ccttcgccaa cagcatccca aaagttggtt atacccggag
    13441 ctactaggaa tccaacggcc tttttatagg gtgcgtaaaa gttatatgca aaatgggtgg
    13501 tttcagggcg gaatggacgt tcagcagaat atacaatatt gaactgcttt accttttggg
    13561 ctttccggtt tatgatgtac agccatgcaa agacggcacc aaagataagc accactatgg
    13621 ctagtataag tttaccattc caatacccaa atggtccaat agctaattgc ccatgccaaa
    13681 ccaatgcacc ttcagggaag tagtttaaca gaatttgccc aattggtttt agcattgagt
    13741 ttggtagcgc tgaaagaacc aatagtactg taattattat atattgagga acaagcatcc
    13801 aaaaaggagc ttcctttacg tttcggagtt catccttggg ttgccccaaa aagatagtgt
    13861 gaataagcct aaaacagtac aggaacgaga ttacccctgc aaaaaaagca attccggcct
    13921 gaaagtacca tccgttttgc atgatggcgt tgaacattaa ccatttaccg ccaaaacctg
    13981 ttaagggtgg caatcccgca attactatga taccaatcag taccgataca aacgaaaaag
    14041 gcatttgctt gatcaaacca cccatttgat acatatactt ggttttggtt cgcattacaa
    14101 cacctccaat tgctaagaat agtaaggatt tgaacatgaa gtgtgtgacc acaattgaaa
    14161 gggatgctaa ccaaccaagg tgcgacatca tggttaaacc aaaaagtgca tatcccagta
    14221 cacctatgct tgaatatgct aatagccttt tagcgtcttc ctggaatgcc gccatcatat
    14281 tacccataag agcggtaaga acacctaccc agcttagcgc ataaaggaca tcgatgccaa
    14341 atagtttttg tggccccatg cttaggaata gcattaccaa tcctaacact ccggctttca
    14401 ggagaattgc cgaaaccatt ggtgatacat cggcctcggc ctcggcgtgc gcgcctggta
    14461 accaaatatg aaggcctatt gacgcaagtt tggtcatgaa acctattgcc attagggtaa
    14521 atgcaatagg agcatagtaa tgtatgtcct ttaatatttc aagccccata ccagtatggc
    14581 ctagctgtgc aatgctaaaa ccagccagga ttaagtaggc tccnnnnnnn nnnnnnnnnn
    14641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14701 nnnnnnnnnn nnnnnnnnnn nnntaccagt atggcctagc tgtgcaatgc taaaaccagc
    14761 caggattaag taggctcctc caaccgagaa aagaatgtaa cttaatgcat gctccatcga
    14821 ttttttgccc ctgataatca ggaagtacga cccagctgtc attaactccc aggcaaagaa
    14881 gaattccagt gtggttttag cctcgagaag acctattaat cctgcaaaca tcatcattac
    14941 aagcgggtaa aatccagtac gtttaccctt aaaagcatag ccggccagga gagtaattat
    15001 accgccaatt acaaatatta taaagaagat actggttaac ccatggaatg tatcttgatg
    15061 tgaaaagtag tacaacacca ttgctccaat ggctaaggtg tttttgatgt aaacaggtaa
    15121 aaagtcaact atataaagga acgcaatgaa tgcggtagcc atatatataa aggtagattt
    15181 gtaacctgta atctctccaa aataaaatcc taaagcgtat gtcaatccag caaataatgc
    15241 agtaggtata aactgatgta gtttgatttt agggtaattc tgttctacct cgtcccattt
    15301 gattgcataa cccaaccacc ggaacaggta aaccccttca aagagcgacc caagcagaat
    15361 tattgctagt aaccagaaat ggtggttact tgccagttgc attaccagtt gccatttccc
    15421 aaaaaacgat gggaatggag ggaatccaac cagtgtaaaa ataaaagttc ccatcaggaa
    15481 aaggtagatt cgatggcctc taattgcacc ccaatccttt aacttatcca catttacaat
    15541 gcctgcaagc caaaacaaac cagctttagc caggtaatgc gagaggagga tggccaatgc
    15601 aatgaacttt gtattctcac caaggtaagg ctttaaccct attatggtca taattaaacc
    15661 aatttgccct accgatgagt agcccagaag ccgcctggtg ttttcttgtt taatcccaag
    15721 caggtttgat gccataaagg ttatggctcc gattatgctt agggcattga aaagggtaaa
    15781 atttccaaac accatcagct tgtccagaac aaaaattcct gcagtggccg atgccgctga
    15841 tattaaagct ccaattccgg gatgtgctcc ttcatacaca tctagcgccc atccattggc
    15901 tgggaagggt tttagctcca aaacaatgga tagtaggact agaaaaattg ctacatatcc
    15961 acctttaacg tataaaagcg acgcctggct tataaagtcg atattcagtg tgccaccaaa
    16021 gtagtaaatg aaaactgttc cgagtagtag cagtcctgag attaagccgg tggcaataac
    16081 atacttaaaa cctgccggaa tcgatttacg gtcagggtta agtattacca aacccgcaat
    16141 ggcaatgctc tgtatctcca taaaaacaaa taggttgaag aggtcgcgag tcattaccat
    16201 tacatttagg cctaatacta gtactagaaa cacaatcatc atatttttgc cagcctgcaa
    16261 tagttcttta gttaggtaga ttgcgccaag taatccaact gtattaataa gaagcgtaaa
    16321 tatcgattcg gctaagccca tttgcaggtt aatggcaaag ggtggtttaa atcccccggt
    16381 aaaaatctga acagattctg ttgcattgcc ccagaaatgg cttaaccaac ctgccgatat
    16441 ggcaatcatg gctgccaggg cggtgtaaac aaccccaagg gctaacttct cccttttacc
    16501 taaaaaccca agcgcaaagg ctactcccag cgctatggta actatatgta ttgggtttac
    16561 catttcaaat cggtgtatga gttaatatca agtgattttt tctccctata cattcgcaaa
    16621 acatatgcaa gcataagggc tgtaacaccc agtccaatta caattgaggt aagaactaga
    16681 gcttgtggca cagggtctgc tactttgctt gcagcttgcg acacatctac agccttatcg
    16741 ataattggtg cagtgctgtt tttaatataa gccatgctaa caatcacaat gttaaggccg
    16801 gtatcgaaca ggctgaaacc aattacaatc tttatcaggt ttttctgggt aatcataccc
    16861 cataagccaa ctactatcag cataaagcct ataagcatgg caagaaactc aagtgtaagg
    16921 tattgcgcca tagctaaatc tcgttttggt tttccttaag tgaatcgagt atgccagtta
    16981 gctcagagcc aaccttaatg ccaatgaaaa catatattat tggaatggcg cctgcgctta
    17041 aaaggcttcc gagtttgccc agtggtaaaa aagcgttgtt taggaaaccg ctagccagaa
    17101 taaggcccag tattcccatt gcaacaaatc caacccctga taccgattca agaaccgata
    17161 tcagcttgtg gttaaacctt gaggtgggtt gggcaagtat tatcatcata acacctgtgg
    17221 caataacagc gccaccttga aaacctccgc caggggtaag gtgcccatta acaaaaatgt
    17281 aaacacccag catgaaaatg gttggcacaa gcagggtgga cgctgtttcc agaatctcgc
    17341 ttgttttcct tagcttgttg ggcctaccat tatcggcatt cattactttt agaaagaagc
    17401 ccacaattgc cgcagcaatg aacaaaatgg tgacctcgcc tagggtgtcg aatccacgat
    17461 aggtaactac cacggcagtt accaggttgg ctgcacccac ttcggcaggc ccgttttctg
    17521 cgtagtaacg cgccagcgga ttaagctcat tgctcccggt aaagttgatg agtaaactgc
    17581 cgattacagc aacaaaccca accagtagta gcagtagtat tcctttctta atcattggag
    17641 tgtcggattt tgtttaaaac gtaaaaaaaa ataatcgtgg aaagtccact acctatcgat
    17701 gcttcggtta gtgctacatc gggtgcagca agtaaaaggt acagtaccga tgcaaaaagc
    17761 ccaactgccc cagtggcaat aatggccacc tttgtgttct tgtggtagat ggcagttact
    17821 gccagcacaa ggataactac tcctagaaat attgcaagaa ttaaactcat agctattcct
    17881 cctcagtttt gttttcgctt tctaccaaga ggtcggcaac cgttttatcg gtcatttttg
    17941 tacccctaaa gtgtgctgcg cgcgaaatca cgttcgatga aaccgggttg gtgataagca
    18001 caaagaacat aatgactagc agtttccaaa tccaggcagg atttactatg gctagcccaa
    18061 ttaggctaag aatggttccc agggtcgacg ccttggtacc agcctgaatg cggttgaaca
    18121 ggtcaggcat gcgaactatg ccaaggccac ctaaaaatat gaagatggaa cccacaaggg
    18181 ttaccgatgc tcctataagt tcacgtattt ccatcacaac cccctttcga tatacctacc
    18241 tattactact acgctcagga agctgagtaa gccgtaaacc agagccacat ccagatagat
    18301 aattctaccg gcaaagtggg agattagagc aattaccgat attgaggcca cggtcatcac
    18361 atcaaaagca atggttcggt cggtgacatc gggacccttt atgaatcgga gcaatgccag
    18421 caggatgccc aaggccatga gcacacctgc cataattaga atggtgttaa ccataaatca
    18481 cctccagcaa cttttcgaat tttcccagaa ttttccggct tgcctcttcg gtatcaagcg
    18541 tttttacatc aatccagtgg atgtacagct catcatcctt catgtcaatg gtaagtgtac
    18601 ctggagtcag ggtgatagag tttgccagaa tcatccgccc tatgggcgat ttaagccgtg
    18661 ttttaacctt tactatccct gggtttatgg gaagtttagg gtttaacacc ctaagcgcca
    18721 catcgatgtt cgattttact atctcccagc taagcacaaa aaggtagatt aatgagtaaa
    18781 cgaacacctt aggggtcatg cgaaacctcc caaaaacgtt gctattcttc ccgaaaatta
    18841 gggcaagagt aacggcaagc gccaagccca atagtagact tgtaagctca aacgtaccgg
    18901 taagcagcaa ccaaatcagg aaaagtaaaa caaacaggtt tgctacattt ctgatattca
    18961 ttggtttgta gtttgttttg tgaattaaaa taaacagata tatagatatg taaaagtaga
    19021 catgttattt gactaacaaa taattaaagc agtaacatat tagggtaaaa agattaaatt
    19081 tgcaaaggtt ttcaattaac atatgttgat aaaaacgatg atttacatca ccgtttcttg
    19141 aattgtaatt taattaacag atggttgagt ctaaaaaatc atgcctgctt tgcaataaca
    19201 agtcggaatg ttttaggcag ctgagcgact ctcagcttga ttttgctaat agcaacaggg
    19261 ctgaaatatc gtaccgcaag ggggagatga ttgccaagca agggaacttt tatacccata
    19321 ttctttactt gcaaagcggg atggttaagg tttacaagga gttgcccgat ggcagtaacc
    19381 ttattttgag catatttggt gtgggcagct tgattggttt acccttcctt ttcaagaacc
    19441 agattctgga ctactccgta gctgcgcttg acgatacaac catatgcgcc atcgacaggc
    19501 atgcttttga aaagcttatt cacgagaatg gcgaatttgc cgccgaggtg gtaaaccagc
    19561 ttaatcagtg tacgctttac aactacgaca ggctggtaag cctcacccat aagcaaataa
    19621 atggccgttt tgccgatacg ctcctttatc tctccagagt ggtttacaag gctaatccct
    19681 ttaaccttac ccttagccgt aaggatttgg ccgaatatac tggggtttcg gttatgagcg
    19741 ttatacgcgc catcaaggaa tttaagaacg atggaattat tgatataagc ggtaataggg
    19801 ttgaaatcct taacttcagt cagctggagc ggataagtag aaccgggtaa ttcgtttagt
    19861 tttacaagcc attgattaaa attgagaata tgttagctga tcagaaggta gatctaaact
    19921 gcaaatggaa ttatttcaaa cctttactaa atccctttgc cgtatgctct ccatttctca
    19981 attagcgcag acatgtcgta tggtagctgc gagtcaaagt gcattttctt gttggtgagt
    20041 gggtgggtaa acccgagcgt tttggcgtgc agggcatggc gggggcatag ctcaaagcag
    20101 ttctccagaa actgcttgta ctttgtggtt aggttgccct ttaggatttt atcgcctccg
    20161 tatacctcat cgctgaaaag tggatgcctg atgtactcca tgtgggcacg tatttggtgc
    20221 gtacggccag tttccagctt acattcaata agggttacaa acccaaatcg ctcaataacc
    20281 ttccagtgcg ttacagcatg cttcccctgc tcgccatcgg ggaatacctg catttttttt
    20341 cggtcacgga tactacgacc tatatgtccg gtaatcgttc cactatcggt ttccatatcg
    20401 ccccatacaa gcgcaatgta tagcctgtca gtgcttcgct caaagaactg ccgggccagg
    20461 tgattcatgg ccatctcggt tttggcaata accagcaacc ccgatgtgtt gcggtcgata
    20521 cggtgaacca gcccgggcct tacttcgccg ctcttgaaga gcggaacgtc gcgcaggtgg
    20581 taaagtaggg cattaactaa tgttccggtg aagttaccat gtgccggatg cacaaccatt
    20641 cctgcaggct tgttcaccac aagtaaagct tcgtcctcat aaactatgtc gatagggata
    20701 ttctcgggtt tgatatcggt ttcgcggggt gggtaagcca gaacaatgga aatggtatcg
    20761 agcggcttta ccttgtacga cgatttaacg gcctttccgt taaccagtat attgcccgca
    20821 tcggcagccc cctgaatacg gttgcggctc acattccgta tcttatcggt aaggaattta
    20881 tcgattcgcg ttggtgtttg tcccttatca acctcaatac ggaaatgttc gtacaggtcg
    20941 gcctgttccg ccgttccgtt ttccaaatca tcgtcggggt caagcagccg attttggttc
    21001 attgcttaat aatcactttg gaagtaaggg actgtccgtt ggcaaggttc acctttagta
    21061 ggtacattcc tgttgggatg tgttcaacat cgataggttc gttctgataa accgatttag
    21121 actcaattac ctgaccggtt atagaaatta gctggtatga atttacagca attccattac
    21181 caacatcgat ataaatcttt tcgcttgccg gattcggata aaccctgatg ttactggtag
    21241 tagatatagg tgcagtggtg ggattttggt aaagcttgcc aaaaataggt ctgatcatta
    21301 tagttccctc aaactgggtg tttgtccagt aaccgggtag ggtgtgaaaa atcctctccc
    21361 gactatttgt gttgcggtca aatccaacat tcaaaaactc aatggtactt tgctgccatc
    21421 ctatgtagaa gttgccaacg ggtatcgaaa cggggtcggt acggtaaacg gtaaagcagt
    21481 tcaggctatc ggtaaatagg gggcgggttg tgcttctctg gtagagtagt tcacccggtt
    21541 tgccattgtt atcggcccaa atggatattt taaagggctg gttattggca tcctggtagc
    21601 tgcggttaaa gtaaatcatt acgcctacca ggttgtcggc cttgtagctg taaaactttt
    21661 gggcaatcat ggcgttaatg gtaccctcgc caaaaaggcc atagccgctt tcggccgaac
    21721 catcgtcgag ggcgtagtag ttgtaaaagt tgagctggtg aaccagcgtg tcgttgtacc
    21781 ttaagtgctt ggttgattca tcaaaatcgg tttggagaaa agttttgagc atgaatttgg
    21841 cggagtcggg ccaagcagag gtgaaggtgt agtcgaatgg tcgggtgtat gtgaattcct
    21901 catacccata aatgttttcg gctcctccag aaaaggcgta ctcgttgccg gttgaaaggt
    21961 tggtaaccgc aaagcggcgg gtaacgttcc atgtggtggg accaaggttg ttgtaaacaa
    22021 tagaaaactc gcggggattc ttaatttcag aagcctcagc atcagcaaaa tgtgtccatg
    22081 gaaccgatgt gtagttcttc agcatcgatt ttattggttt gcgaaacgca atgtcgttgt
    22141 atatggtgtc gtcccagtta cgtagcctgt tcaggtaaac caaatcaata tgccaatggt
    22201 cggagttggt tttgtagccg ggcacagtgg cattctccat aagcgaagca tacccaataa
    22261 agcgaaaacg gaaaccgttt tctaagaaaa tgggattatc aatattgatg ttaactgtaa
    22321 agaatgttga gtcgagctta gtggaacttt tttcatcctt attgttgagc aggtggttat
    22381 agaaagcaat cttgttagcg ttgaagtcaa ccgatgaagc ccagcctcta acccagcggt
    22441 tttccgatgg gctatatagt tcaagcacca gcgagtcatt tgggctgggt tggtaaccca
    22501 accctccggg ttgaacgttg aacgacaggt aaatgctatc ggttgcaggg tagtttaggt
    22561 ttatggggag cgatgtaagc gtatcggcag gttgcggggt ggtggttagg tgctgatgta
    22621 aaactccctt gcggttgact gcatcgaatg ttactgcccc aacggtagga gggttaatgg
    22681 cataggtctt attggatatt gcattgctag gttcccagag ctctggggtg ggatagggag
    22741 tgtttcgggc aaaatcgtcg aaaaatggta aggacagatt cgtttttgtg taattacttt
    22801 tcttagtaac atcacttatg gtacgatagc ccgaaaggtt aaccagaacc tcctgcccgt
    22861 agcataggga tgatacaaat atgaatacta gaatggttaa tgctttccgc attgtattgc
    22921 tattcaataa tttcatcggc atgcttagcc ggcattgtgt taaccgagtc cgcacgctca
    22981 atctttggta tgcgtgcctg gtttagtgta agccatatat caaccctagt tccaaagcca
    23041 agggttgcgt tctccatgta ggcagggtac tggctataca ccctagcatc taacgagtcg
    23101 ttgtgcgtaa ttattgtttc gtcgtacctt attctaccaa cattgagcga tgcctcaatt
    23161 attctgctta ctgcatcgtt aagtttaaga cctatgagta gcggtattcc tgtacgttcg
    23221 ccctgcatac cctgacctaa aaccaggtcg atttttgagc ccttggggat gcgtgtgccg
    23281 ggttccgcaa ttttaccttc aaagcgttgt tcaagaacat tgtttagccc taggtcgggg
    23341 cggaagtata gggtgccaac ctcaaagcct tcctgctcaa ggatagcctt tgcttgtcta
    23401 agcgtgtagc caacaatatt ggggatttca attttgatag ggtttttggc gttgatggta
    23461 acaaataccc ttcggttctt tttaaccatg gtaccaggtt cgggattctg ggaaattacg
    23521 cttcccggtt tcttgttaaa tatgtaaacc gaatcggcta tttcaatcct caggttgtta
    23581 tcggcaatca cctcagctgc ttcctctact gccataccca aaagtgttgg tgtaggttgt
    23641 tcctggttat gcctggtgta tatccttaga aagatcatga gtacaataag tactccaaga
    23701 atcaacccta atccatttat tatggtgtta acgtagggga ttttgtatat cttcagaagg
    23761 tactgtttta ctttctcgaa tgcagttttg gttgtcataa tgcaaggtaa ttaaacatga
    23821 tacaaaaata gcataaacat gtaataatgc taccaattag ctgtatacct taataatatt
    23881 atatagcgaa tcgcgctcgg ccggaatctt acccgcttct cttacaagtt gtgtcaaccg
    23941 gttaactgtc ataacaggct tttgctcttc ggctccggcc atggagtata tctttgtggt
    24001 atcgtcgata gttccgtcaa tatcatcggc gccaaaatgg attgcaaggc gggtgttctg
    24061 tatcccgagc attggccagt aggcctttag atgcttaata ttgtccataa agattcggga
    24121 tactgcaaaa ttgcgaaggt cctcggttag gttaacctcg cctaggtatc ccattgggtt
    24181 gtttgcggaa cggaacttaa gagggataaa gcagttaaac ccatgggttt cgtcctgaag
    24241 gttgcggatg gcctccatgt gttggaccct atgcttgtaa ctctcaatgt ggccgtaaag
    24301 catggtggca ttggattgta tgccattttg atgtgctgct ttgtggattg ccagccatac
    24361 ctcgcccgat gctttatcgg ggcatatttt ggctctcacg gccggatcga aaatctcagc
    24421 acccccgccg gggatggaat ctaaaccata ctctttgagt ttcctcaaac cctccgcgta
    24481 cgaaagtttg cttttacgaa tcacatagtc gagttcaatg gccgagaagg ctttaacgtg
    24541 aatgttcgga agtatctcct taacggtttt gatcattttc ccgtagtagt ccaaatccca
    24601 ttttgggtga acaccgccaa caatgtgtac ttccgtaaca tcgctgtttt ggtagttgtg
    24661 ggcaattttt ttgatatcgt caagattgta aacccagctc tccaggtcgc cagcaccctt
    24721 atggtacgaa cagaatttgc agttaaaaac acaaaggttg gttggctcta tgtggaagtt
    24781 cttgttaaaa agaacgtaat cgccatttat cctgctactt gtaatatttg ccaattgcga
    24841 tagtagggct aaatcggctt tctcgtaaag gtacacagcc tcgtttggct gtatacgttg
    24901 cccatccatt accttgcttg taatgtgttt gagaaattca tcgttgatgt tgggtgctat
    24961 tgtttttaca atgggttcca tgttaaaaat ttgcggtaaa tatacaccaa gaaaacaaaa
    25021 aaggcaggtt aattacccgc ctttatctgt tatgattgat tagtaattat ttccggaaga
    25081 tttcatcgga aacagtcagt ctttttcttc cctttgcacg gcgggcagcc agaaccctgc
    25141 ggccattgtg ggtcgacata cgttcacgga atccgtgttt atttcttttt ttcctgatgg
    25201 atggctgaaa tgttcttttc atctctgtat gcttttacta attattattt ccagcaaatc
    25261 tatggctttt ttaaaccctt tttgggttcg caaaggtaaa acaatttttc aatattaaaa
    25321 aacacctggt taaaaattca aaaaatagtt agccgcacat cccttagcca ggggtttggt
    25381 ttaggtactt gataaccgct gagttgacac ataggcgact actataatgc tcaatccttt
    25441 aatgtgcatt ttattgataa ccaatgtact aaaaattatc aaaacttata ttgcaccatt
    25501 taggccagat tttgaatcaa cccacttttt cctttatccc ttatccttta tcctttaacc
    25561 tttatccttt aacctttacc tcacacctca atatgtatca catacttttc aacgaagtag
    25621 tcgtcctcaa aaaaatcgtc aatattccat tttgtagccc acttgtggta gggctttagc
    25681 tcctctttta tgtcgcctcc tttaagacag attaggccgt taggaaggtt gttaatgccc
    25741 ccggggtgaa tgtttttttc aacccaagcc ataagctttg gaacaggagc cactgcacgg
    25801 ctcacaacaa aatcccactc ctgttttaga tcctcagcgc gggcggtgat tacctccaca
    25861 ttatgtaagc caacggcttt ggctacctcg gaggttacct ttgttttttt agctatggaa
    25921 tcaaccaggg taaacttaca gtttgggaat aggatggcaa gggggatgcc cggaaaaccg
    25981 ccaccagtgc caatatccat aatggttgtg cctttggcaa actgaacaac ctttgctatg
    26041 gccaaggaat gcagaacatg gtgaatcatc aggttatcga tatccttacg cgagataacg
    26101 ttgatttgtg agttccatag cctgtaaagt ggctcaagct gtgcaaactg atcgcgttga
    26161 gcttgagtta gctcaggaaa gtattgtgca atttgttcca tggcaaaatg tattatttat
    26221 ggtgtggcgg aaggttgctc aggatgtttg agagcagctc acgggctctg aaaagttgag
    26281 cctttacggt tcctaacgga aggtctaact cttcggcaat ctcttcgtag gagtactcct
    26341 taaagtagcg tagctctatg agcttgcggt agcggggttt aagcttggaa accacttcct
    26401 gtatcaggcg tatgttctgt tccttaatta gattttcctc aggatcaggt gtcgatgccg
    26461 gtataacgga cgatggcgac atggagttgc cttcggcatc ctctgggcct tggtcaatcg
    26521 atatcaggtt ggccttgcgc ttgcgaatga agtcgataca gttgttcgat gcaattttga
    26581 atagccaagt gctaaatgca aaattcggtg tatactggtg tatgttcttg aaggctttgc
    26641 caaaagcctc aagggttaaa tcctccgcat cgcttttgtt gttaaccatt ttcagcagca
    26701 tgtagtatat ggcatcccgg tagcgatcca taagctcagc gtaagctttc tggtcgcctt
    26761 taattgcttt atccacaagc acaaggtcgt atttcgcctt ctccgaaagg ttttccggga
    26821 tcattaccat ttctgctgtc gtcggttaag agtgttaaag attagcaatt tagcataaaa
    26881 atagagagaa aacaaatccc accaaaatgc aatatgcatt agcttttttt cttcaagcct
    26941 ttttgctgca attgcagcta tagtgtgttt tacaataatt accagcaaaa gtaaagcagt
    27001 tgatatcaaa ggaagaattt tgaaaattag ggttaacgca aatagaatgt aaacaagtat
    27061 tctgcttaat ggttcaagcc ctaaacgggt tttcaggttg ctgctgtaga atttggctgt
    27121 tgataggtgc ctgcgctttt ggcgtaacca ggtagaataa gtttttcgag ggactgagag
    27181 agtatgtgcc tcagggcgat actcaacagc cgtattgctt ggtgttgcaa cctccatcac
    27241 aaaaaggtca tcgtcgccgg agtcgatacc ggcatggttg gcaaaccctt tatttttgaa
    27301 aaaaagcgat tttttgtagg ccaggttacg gcctacgccc atgtatggcc tacgggctaa
    27361 cgcccatcca aagtagttca aagcgatgaa gagcgtatcg agcctaacta gcttattgag
    27421 taaacccctt tcggccaggt atccgccata gcctagtact acctctgtgc cattggtgaa
    27481 gttcgacgcc atgtagcgta gccacttgtt cgattttggg aagcaatcgg catcagtaag
    27541 caccaaccat tcgtttttag ccgcctttat gccaaccgta agggctaatt ttttggcatg
    27601 ggtaaatttt tcgtcttcct taatggttgt tacacgcagc cgagggtatt tcttttgcaa
    27661 gctctcaagt accagatcgg aattatcgct tgaacagtcg ttcactacca ccacctcgta
    27721 gtcagggtag tcctgctcaa ggactttggg aaggaaatgc ttaaggtttt cctcctcgtt
    27781 gcgggcgcat attactaccg atagaggtgg gtactgttca tcgttttcgg gtaaatcatt
    27841 aaatttttta cggataaccc ttaggtagaa aaccaggtag aagtaaagct gtataagtac
    27901 aaatactaaa aagacaacac caattacttt ttggtaaatg cttaaattat agataatttc
    27961 cattataaga caagtagtaa ttttcatgca atgatattaa attttggctg aatttcaagt
    28021 gaagtttaat catgatttgt aacaaatggt ttcaaatttg tttcctgtca gtatttgtga
    28081 gctcaacata gccatgttca attactttac tacatttgtg aaaaatttat aaagatttga
    28141 gttttagtag atttaattta aaccctgcgg ttcttgaagg tatcgaagcc atggggtaca
    28201 caaaacccac tcctattcag gagcaagcca tacccgtaat tttggaaggt cgcgatttac
    28261 tagcctgtgc gcaaacagga actgggaaaa ctgcagcatt tatgctgcct atcctcagcc
    28321 tcttgtcagc tgaagaacat gatgcttcgt cggtcaatac gcttgtaatt gttcctacac
    28381 gtgagcttgc catgcaaatc gaccaacagg ttgaagggtt ttcgtacttt gccccggtta
    28441 gctcaatagc cgtttatggc gggaacgatg gctctaactg ggacaggcag cgacaggctt
    28501 tagagcatgg cgcagagata attgttgcca cgccgggccg attaatgcag catattcaaa
    28561 tgggctacgt taaccttaaa ggcgttcagc acctcgtgct cgatgaggct gatcgaatgc
    28621 tcgacatggg attttacgac gatatcatgg caataattgc ctatttgccc aagcaaaggc
    28681 aaaccctgat gttttcagca accatgcccc ctaatatacg taaactggca aaatcgatac
    28741 ttgttgagcc agttgagata aacctggctg tttccaagcc agctgaaaat attttacagg
    28801 cggtttacca tgttggagcc tcgcaaaaac tgtcgctttt ggcctcgctt ttgagcaaga
    28861 aggaggtggt gcagagtgcc attgtttttg cgtcaaccaa ggccgaggtg aaaagcattg
    28921 aaagcaaact acacaaggaa gggttaagcg ttgctgccat gcactctgac ctggagcaga
    28981 aacagcgcga ggaggtgatg cgcaattttc gtaaccgtaa ggttcaggtt ctggtggcta
    29041 ccgacattgt tgcacgcggt atcgatatag aggatatcga tctagtggta aactacgatg
    29101 ttccgtccga tcctgaagat tatgttcatc gtattggtcg tactgcccgt gcccagtccg
    29161 atggcgtagc cattactttt gttagccccg accaacgcgg gaagttgcat ggcatagagc
    29221 gtttcctggg tacaaaaata tttgtcatca actatattcc taatagctaa gcttaggcaa
    29281 tgaagaaatg gttactccga atagtattcc caatcctacc ggtagtaatg ccataatttt
    29341 catttggtca ggttattaag ggtcgagttg ccgacgatac cggtaggcca atcccttacg
    29401 cttcgatata ttccaaggaa cttaggcagg ggacatcatc taactccgat ggagactaca
    29461 tgcttagggt tcccagtggt gagtattcct ttgtttttcg gggtcttggt tacgaaactg
    29521 ttgagcggcg gattaaagta gatgtaggtg aggtaaccct aaacatcacc atgcctgtaa
    29581 agccatttct gattgcccct gtaaccgttg gaggcaaggg cgaagatatt gcatacgatg
    29641 tggttcgcaa ggcaattgcc atggcgccat actaccgcaa tctggttagt gagtacgagg
    29701 cggaggttta ccttaaaggg actttcaagg tcgacaaact gtcgtggttg gttaagcgaa
    29761 gcataaagga tagcccaaat ccgcccaagg agggttcgct ttacctgcaa gagtcctaca
    29821 acaacgttag gtttacagcg cccgaaaaat ttgatcaaag ggtaaagatg attcgttcca
    29881 attttcctgg cgagttaaac agcaccgata atattctggg ttttgtttcg gcaaatctat
    29941 accaacctaa ggttggtgaa atcattttac ccctagcgcc ttacgctttc aaccattacc
    30001 tgtttaagta cgagggttac gccttccagg ataaccgcgc attggttaag gttaaggtta
    30061 ttcccaagcg aaaaagtcgg caactggttg agggctacat gtatattgcc gaggattact
    30121 ggaacctaca tgaaatcgat tttactgttc agtcgcttgc ggggccattt cggattaaac
    30181 aaacctttgg ggaggttgag cgtaatgctt ggcttcccat tagccagtat tttgatattg
    30241 atgctaattt ccttggcaac gttggccgaa tacagtatgt tacggctatc aagtatctga
    30301 atgtaaaact aaattcaaaa gtaaaaccac ctgttaagat tgatataaaa gaagcggaaa
    30361 ccaagcatat tgccgaggat acacaggtca aaagaaatac ttcgccccga aagcaaaggg
    30421 aagatgcccg tatggaacaa ctcttggcca aggataatct tacaaatcgt gaaatgtatg
    30481 agctgcaaaa gctcatgcaa aaacaagtac gccaatccga ttctcttccc aataagcttg
    30541 aggtgcctaa ccctctaacc attacagtcg actcggttgc aaccaaaccc gacaccatgg
    30601 cgtggcagca ggttaggcct gttgcgctta atgccgaaga gttcaaagtg aatagggaga
    30661 tagatagtaa gctacagatg tcgcccgata gcgtttcagc cgatagtacc ggtaaggcaa
    30721 atgtacttaa aaccctttac atggggcaca cctggtataa cgccaataaa aaacgtacat
    30781 ttctattctc gggctttgct ggtccaaatg agttccgctt caacactgtt gatggttttg
    30841 tagtaggggc ttcgggtagc taccgtaagc agtacagcag gaatggaatt tatatcaaac
    30901 aatcggcata ctatgcaact gcccgcaaaa cgctcatggg tgaaaccgat tttcggcttt
    30961 cctatgccaa tatgaatagg ggtttagcat ccatccgaat gggttggata agttacgatt
    31021 tcaatggcga tgcgggtatt agtccattaa ccaatacctc ggcctcgctt tggtttggcc
    31081 gaaactacat gaaactttac gaaaaccgat atgtgtcgct ttcgaaccgg attgacccat
    31141 taaacggatt tgaggtttac acctcggttt cgtttgcgcg cagggttgag ctgcaaaata
    31201 ataccgattt tattgtttac cagcagaacc tgcgatggta caccccaaat gttcctgcta
    31261 acgaggatgc cacaccccag aacctatcaa ctcatagttc gcttgagggt tccattcggg
    31321 tgcgctacac tccgttttac cattaccgaa ttcgcaatgg tcgtaagcaa atgctttact
    31381 cccgctggcc taccattacc tttcaggttc ataaggcttt acccaattta cttaattcaa
    31441 attccgatta tctcagatgg gaagccatgg ttaatcaaat aatcagaacc ggtccaagta
    31501 atcgaatttc ctataaattt atctacggtg attttgcaag caaggggagc ctttactttg
    31561 tggattttaa gcatttcaac accatgcggg tttcggtggt aattccctct tttagcgagg
    31621 gttaccagaa tatcagttac taccgcagga gcacatcgga gtgctatgcg caagcttttc
    31681 tgagttacca atcgccgtac cttgctttaa agtatttgcc attcctgagc aatagaattt
    31741 ggctggaaaa cttgcagttg actttcctta aaacagcagg ctataagcct tactatgaga
    31801 ttggctactt tttaagccag ataggcgttg tggggggtat tggtgttttt ggatcgtttg
    31861 agggtaagca attccactcg ctatcggtaa aggtatcgct aattattcct ggctttgagt
    31921 agtatgctaa actgtttgtt tagtacttag ggggggctgc atttgttttt ttttacattg
    31981 aaatctaaag catataatct atttttttaa cggtttagaa ttcagtgtaa tatcattatt
    32041 tttttgtgtt ttttgtaaaa tgaatgacaa ttcattaata ttgccacatg agaaaaaagg
    32101 acgaagccaa accggaagta atctttgagg caagccttga tgtacttata aatcatggtt
    32161 ttgtgaactt ttcaatggga agggtggcca aacaggcaaa aatagctgtg ggtaccatct
    32221 atctttactt taagcataag gatgatttac tcaatcaact ttactttaaa ctttacaaag
    32281 agagtgtcga taggtttttg gagcactaca atcccaataa ctcatttaga gagagtctta
    32341 aactggtttg ggtcaattac cttaagcatc gcataaatca ttacaaggag agccacttcc
    32401 ttgaacagta ctataaatcg tcatatatta acgatagcca taaacaaatg gcagagcaga
    32461 tgaaaaagcc aatacatgat atcattcaaa agggtaagga attaggtctc gtgaggagcg
    32521 atttggataa tgaaatgatt tttctgggga tgcttggatt tctcagggag ttagccgatg
    32581 agcatgtagg taacgtttat attcttgatg aagataggat taataaagcc tttgaaatga
    32641 gctggaatat gattaaagca tgattgtaaa acttaaattt tgatgtttat ggcaatacca
    32701 ttttggcagt atttggtttc gatgccactt tacattgtac tgttaatgct ggcagtggag
    32761 tttatgcgaa cgaattacag gtttgcagct gtattctggg tattatcgct ttttactttt
    32821 cctctttggt ttaacaacct tgagggttgg ttccgttggg caaaaacact ttcggtttta
    32881 ttgcctacag cctttgtggt tggctgtgcc cgcattgccc aatacgaaaa acgcgatgga
    32941 tggtggacaa tctttcgaaa ggattgggtg ttatggtttc tgtatgctat tcttgggctt
    33001 aacatactgg aagcctcttt gaaggatttt gaaatgggca attggttcaa tggcctatcg
    33061 ggattaatac ttattgtaac gataccgctt gtaaaatcag gaaagggtaa acggtatgga
    33121 tggaagattt ccgaggagaa acctggcgac ttaattgctt acaccgatgc tatatggaac
    33181 ttcctatata ccagctggaa tattgctttt gtttatgctg aaaatcccgg atacgcagca
    33241 agttcgcttt gcatcctatt agcagctgaa ctttatcctg taattaaacg cagacccgaa
    33301 ttatatgttc aagcccgcgt atatacctta gccattcaca ttcttattag agctacatat
    33361 gatattttta cgccaatcat ggattcgtcg gcatttgcaa gccaaaacgt tgtattttgg
    33421 tggggtatag ttaacttctc gcttcatttg ccatatctta tttggtattt ctttttgaag
    33481 aaaactgaaa agtgattgtt ctttaataat gtagcactaa ttgcgcgcat cgactgatgc
    33541 gtgcaattat tttgtatagt tttcatattg ataattttga aaccagttaa ccgttggcgg
    33601 gtttacttta cgaagccgaa tagattaatg ttgaattgat tttatgaact aaattgaaac
    33661 gtgtattggt tttagcttga aaattattta gatgttgtat tagtgcactt gttttttatt
    33721 gctccgtttc aatcttcttc cttttcgtta ttcgttttcc ccttcaccac aactacaaat
    33781 tcccctttgg gagatttgga gctgaaatgc tccttggctt gggcaagtga tccgcgaaag
    33841 ttctcctcat gcaatttggt aagctcgcgc gcaatgcaca cctgcctatc ggctccaaaa
    33901 atggggagca tctgctcaag cagtttaaca atacggaatg gcgattcgta aagtattatg
    33961 gttcgttcct cgtcctttag tgcttcaagc cttttattgc gtcctttttt ttgggggaga
    34021 aatccttcaa atatgaatct gtcacagggg aatccactct gcacaagtgc aggaataagg
    34081 gctgttgctc cgggtaggca ctccacctca ataccccgct caacacaagt gcgaaccagc
    34141 agaaaaccgg gatccgatat gccgggcgtg cctgcatcgg atattagggc taccgattca
    34201 cctgcctcta ttcggcttgc aaggtaatca accgtttggt gttcgttgaa tttatggtgc
    34261 gatttaagtg gagtagcaat tccgtaatgc ttgagcaggt tgctactggt gcgtgtatcc
    34321 tcggcaagaa taaggtttac ggtttttagc acatcgagcg ctcgcaaggt aatgtcggca
    34381 aggttgccta ttggagtggg aactataaag agttttgaca tgaagcaaag ttattacgat
    34441 agttttcttc gaaccaagtc aacaaataaa gtggtaacct cattggttgg gtcccagcta
    34501 aaattctcct ggaggtaaat aatggcctca tttaaatcag taagctgatt gaggtgttta
    34561 atggtttggt tgtaaaggtc ggcatcgccg ccaaaaagtt ccttgatgaa taggaactta
    34621 tcgttaatgc caattgcctt ggcaaggtcg gttatgggtt tattctgcag tttgctctgc
    34681 atgtcaacct tttggtgttg ctcggctatt acctcgttgc ggaacttgcg cttaccctgg
    34741 tacttatcgg caagtatggc tgaactttcc tcgtgcgatt gctgtgtggg agccggagtg
    34801 ggttttgggg atggacttat gtcagccttt acagggtttg gttcctgctt agctgtcgga
    34861 atgctggaag gttctgcgct ctcctcaact actgttgttt ttttagtttc aggtatgctt
    34921 gctatagttt tctcatgtgg ttcaaccatt tttccggacg gaactacttg tggtttctca
    34981 tgggttctgg aagcaacttg ttgcctatca agcagaaggt cttcatatat tgaggccagc
    35041 ttggcaagta gtatatcgcg ttcaatggaa ggtagttttc cggctgaaag tgccttatcg
    35101 atcaaggcaa ctgcttcaac tagttgattc cgtgcattct ttaggtattg cattgcaaat
    35161 tgttttttgt tcgcatttca aaaataagtt ttattacatt tgtctgtaaa actaactcaa
    35221 aatatttatt taggaaccaa tgccttttct tgaaaacccc gttagtggga aacgtaaggg
    35281 atatatcgag gttattgccg gctcaatgtt ttcgggcaaa accgaagaac tgattcgtag
    35341 gcttaagcga gccaagtatg ccaaacagag ggtcgagatt ttcaagccca tgattgacac
    35401 ccgatactct gagcaggagg ttgtttcgca tgatgccaat tcaatacttt ctaccccggt
    35461 ttcatcgtcg gggaacattc ttattctggc acagaatgtc gatgtggtgg gaatagatga
    35521 ggcgcagttt tttgatatga atttacccaa tgtttgtagc cagcttgccg atatggggat
    35581 aagggtgatt gtggctgggc tcgacatgga ttttcgtggt aaaccttttg gcccaatgcc
    35641 aaaccttttg gccattgcag agtacgttac taaagtacat gccatttgtg ttaagtgtgg
    35701 cgaccttgcc caatactcac atcgtaaaac cgatgccgac aagttggttc tactgggcga
    35761 gaccgacatc tatgagccgc tatgcaggct atgctacaac gaggtaaccc gtaagaatag
    35821 aaagtaggct ttttcgaccc gatgtataac atcagcatca tgcaaaaaat taacaggttg
    35881 gggaaattgc tagggccagt tggatccatt gctgggtatt ttattgcttt ggtgggaatt
    35941 attcaacttt ttagctcctt cctggcaata gttttgatta tcattggact attcattggt
    36001 tttacctctt ttgctgccca ggttgatttt ggaaataaaa ggtttcggaa cgcattgttg
    36061 ctttttggaa taattcctat tgggaaatgg caaagcgttg atagctcaat gggtttgagt
    36121 gttaaaaggt tgaatagggc atggaggaca tacagctcaa gtaataggtc gttcgatatt
    36181 gctgatagcg attaccgtat tgtgattgtt gataaacaag ggaaccctct tgccgatatt
    36241 caacgagttc aaagccctga aaaggcaaat gaggcactgg tttattactc cgagcagttg
    36301 ggtttgaata tccttaggcc aagtaggtag aggatttttt atcatctaca tcgatgaaaa
    36361 cccaggttga catatcgcat cgcatatcgc caaaactttt ttggtttgtt actttgatga
    36421 taattactct gcctataacc acacaatacc gtttgttgat ttttggggag tacactaccg
    36481 gtgaggttgt tggagtggag tatgaaaaaa cattaggaat atcggcattt ggccccgata
    36541 agtatcctgt aatagttttc actacgcttg aaggcaaatc agttcagttt tacggccctg
    36601 aaaattttga gtaccccaaa ggtactatgt tgaaggtggt ttacagtccc aataacccca
    36661 ccgattgcat ggtttttagc ttcggggctt tatatactga ccgaaaagcc ataatcccgg
    36721 gaatactttt tttactttgg atggctttct accttgcttt tagaaacaaa aaatagattt
    36781 tgtaattttg ttgaattata ttgcaccaat caattttaaa cattgatgat taacctaact
    36841 gctatacagc ttactgaaat tgttcagggt caagtaattg gcgattcggg ttttactatc
    36901 agaaacgtta ttaccgatag tagagtgcct gtatcgggaa acggctccat gtttattgcg
    36961 ctggttggcc caaatcacaa cggacacgag tttatcggtg aactttataa tattcgaaac
    37021 ataaaggcat accttgttga aaagggtgag gttgactaca caaattaccc gaatgctacg
    37081 tttgttgagg ttgacgatac cctccaggcc ctacaaaaac ttgcggccta ccaccgcaac
    37141 cgattcgttt acccggtaat tggtattacc ggaagcaacg gaaagaccat agttaaggaa
    37201 tggctcaacc agcttctttt ctcggaatac caaattgtgc gaagcccgcg gagctataac
    37261 tcacaggttg gtgttcccct gtcggttctc caaatggata accatttcga tttagcaatt
    37321 tttgaggcgg gtatatccaa accgggtgag atggttaaac ttcagcccat aattgacccc
    37381 actattggta ttttcaccaa catcggcatg ccccatcagg agaatttcga tgatattgat
    37441 gaaaaggtta acgagaagct gaagctgttc cgcgaagtta aaacgcttat ttactgcaag
    37501 gatcacgcgg taattgatgc tgttgttcag gataacactt tgcttgccga taaccaactg
    37561 cttacctggg gacaaaataa ggagtcgaag gttcaaataa tgagcgtaaa gcgcgaagct
    37621 gggaagactc tggttcatgt tcgctttaac aatcacgagc acacaataac gattcctttt
    37681 accgatgctg cctcgtttga gaatgccatg cactgcgtgg ctaccatgct gctattaggt
    37741 tatagcttac aggatatcga tacgcgctta cagcgcctgc aacccgttgc aatgaggctt
    37801 gaattaaagg aaggtataaa cgattgttca gtcattaacg actcctacaa ttccgatttg
    37861 gggtcgttag ccattgccat tgactttttg atgcagcaaa agcagcacga acggcatgta
    37921 gtaatacttt ccgatattct ggaaagtggc tatgcacctg aacagcttta ctcaaaggtt
    37981 gcccaaatgc tcgaggataa aggggttgat ctactcatag gcattgggcc tgaaattagc
    38041 cggtacgact atgcctttga gattgaggcc gagttttaca gcagcacaca tgagtttttg
    38101 ctcgatctaa agcgtaagga gattcgcgat gccgctatac tggttaaggg tagccgaaag
    38161 ttccactttg agcagattag tgctgcgctt gaacgaaagg ttcaccgaac cacactcgaa
    38221 attaacctga atgcaattgc ccataacctg aactactacc gcagcttgct gaaccccggt
    38281 gttaaagtga tggctatggt gaaagctttt tcgtacggca gtggctccta cgaaatagcc
    38341 aacctactgc aattccaacg tgtcgactat cttggtgtgg cctacaccga cgagggtgta
    38401 tcgctcaggg aggcaggtat ttcgttgcct attgtggtgc ttaacccatc gtttgggact
    38461 tatgagctga tggttgagtt taaccttgag cctgaaatct ataacattac aggcttaaag
    38521 gagtttatca acgttttgaa cagggttggg gtaagctcgt acggtattca tattaagatt
    38581 gatagcggaa tgcataggct gggttttact cccgaccagt tggatgagct tatagccata
    38641 ctaaaatcaa ccaattcggt tagggtaaaa agcatatttt cccatttggc agccagcgat
    38701 gagccacatc acgatcaatt tacaattcac caaattgaaa ccttccacaa ggcttgtaac
    38761 gtgttaagtg atgtgttagg gtatcaaccc atacgacata ttttgaacac tggcggtata
    38821 gagcggtatc ccgaggctca gttcgaaatg gttcgcttag ggattggcct atacggcata
    38881 agtcctacgc atggcgataa gctaatgccc attagtacgc taaggagcaa ggtgattcag
    38941 gtgaagcaaa tcaaacccgg cgacacagta ggatatagcc gaaagggaat agttgatacg
    39001 cctacaacgg ttgtaaccat tccggttggt tatgccgatg gcttatcgcg tatgctgggt
    39061 aatggcaatt acagcatgat gctgaacggt accttggtgc caaccatcgg caatattagc
    39121 atggatacgt gtaccctcaa tgcaaacggg gttgaggtta aggagggcga cgaggtaatt
    39181 gtttttggaa ataaacctac cataaccgac atggcaaagg ccatggaaac tattccgtac
    39241 gaggttctta cctcaatttc gcagcgcgtg aaacgggttt actttcagga gtaacctatc
    39301 ccaaatcgct aatgcgttcc aacctatgat ggtcaaggat ttttatcctg cgaccgtcaa
    39361 gttcaattac accttcctcg gcaaagcccg aaagggttct gattgcattt gaggtagtca
    39421 tgttggagag gtttgccaaa tcctcgcgcg ataggtaaac cttgatggtt ttaccgtcgt
    39481 cctcaagccc gtaggtatcg cgcaggaata tgagcgattc agccaatcgt ccacggatat
    39541 gcttttgggt tagggttacg gtgcggttgg tggagaatcc cagctcggtg gcaaccgatt
    39601 tgagaataat tagcgtaagt tctgcgttgt tccggagaat ccccataaag gcattctttt
    39661 caatgatgca caccacggaa tcctcaagag cttcggctgt agcggagtag ttctcctcag
    39721 caaataatgc cctgtatcct ataaaaccga taggcttggc catgcgaaca atctggtcgc
    39781 gaccgccaac tccctccttg taaagcttta ctttcccctt tgagaggcat accaaaccct
    39841 gtggggtatc gccttccttg tatattatct cacccttgcg gaaatactga caggtgatat
    39901 tattgcgcat tatctccctt tcctctggtt ttaggttagg gaaaagtccg cacacccctt
    39961 caaatacgct ttcaatatcg atgttgtagg aatttctgga catttttttt tcaaaaatac
    40021 gtcattttat gctttcaaac ataaaattaa cataatttta gcatggatgg ttgtgttttt
    40081 taatatttac gggctattta gctccattta gtaaccaagg gcatttttcg tccatagcca
    40141 aatgcctttg atgaaaccct aagtattggt ggcgactggt agcgcttata ctcattcatg
    40201 ttaacaagcc ttagggtacg gtttaccgta tcgctgggaa agcctaaacc tacaatttct
    40261 tcggcggaga ggccttgctc aatgtatgca aaaagaatgg ggtcaagtat ttcgtagggt
    40321 gggagcgagt cggaatcctt ctggtcgggt cggagctctg ccgatggcgg tttttgaatg
    40381 gtgtttatag ggattatttc atcgttgcgg ttaatgtagt tggccagcct gtaaacatcg
    40441 gttttgtaaa catcgcctaa aacgctaagc gcaccgcaca tatcgccgta aagcgtgccg
    40501 taacccacgg cagcctcgct cttattgctg gtgttcagca gtaaatgacc aaacttgttg
    40561 gagatggcca ttaacagggt tccccgaacc cttgcctgta tattctcctc ggtgatatcc
    40621 tcgggtcggc cctcaaaaat agagcttagc tggtttttaa acgactcaaa aatgggctgt
    40681 atggaaacta tgtcgtattg cacgccaagt ttttgcgcaa gctctacagc atcgtcaatg
    40741 gagtgctgcg atgagtactg cgatggcatc agcagaacac ggatgttgtt aacatcaaga
    40801 gcctgtgcag caagggctaa cacaactgcc gagtcgatac cgcccgaaag cccaagggtg
    40861 gcctttgtaa accctaactt accgaagtaa tccctaatgc ccataaccaa ggcattgtga
    40921 atacgctcaa tccgttggtc tgaagttgcc gatatttcag ggggtatgga ttggttgcgc
    40981 tccaatacgt tagtgtcaat caccctaaaa tcttcctcaa agcttttcag cttttcaatg
    41041 atatcgcccc cggcgttcac taccatggag ccaccatcga aaagcagatc ggtattacct
    41101 ccaacctggt ttacatatat tacgggcttg gaatatcttg ccacattgct ttggaaaacc
    41161 tgtaacctgc gctcctcaac gttgtagctg aacggcgaag ccgatatgtt tatcaccata
    41221 tcggggttca actttaccag gtgattcatg ggcgcatcgc ggtaaagctt gcttctggaa
    41281 aaagctgtag tggtgggctg gtcatcccaa atatcctcgc atatggttat ggctattttt
    41341 attccatcaa tttcggcaac ccggtagttg ccggggttgg gctcaaagta gcgatactca
    41401 tcaaaaatat cgtaatcagg aagtagtgtt ttgtggtaaa catccagcac ttttccctcg
    41461 cccaaaaggt atgcggagtt aaaaagcaac ttgcccgtag tatcagtatt gatgctgggg
    41521 accccaacta tagcataaat tccattgcat tgctgtgcaa tctggtttac ggtagctatg
    41581 ctcttatcca caaaatgctt gtgggtgagc aaatcgtggg gtggatatcc acacaccgat
    41641 agctccgaga atacaactaa ccttgcaccg tcggctttag cccggtttat cgatgcaatt
    41701 attttatcgg cattactgtc aaagtgacca atggtgtagt tcagctgtgc aagcgctact
    41761 ttcattttgt attatccaga gttaaaaaat tataccaacc ttcaacgata ggctttgcga
    41821 tgtaatggtt gagttaggca gttcgataat tctggttaaa ccatcgcacc aggttatgcc
    41881 aaacagaatg gcgctctcgc cgccgagtga gtattcacct ccaattccta tttggtatga
    41941 ggcaaacccc catgccatat cggttttggt ggtttcgcgg tcaacatggt tggtttcgtt
    42001 ccatgcgtag cttcttattc tgaggctccc gctaacgcca acattggcaa agtatgtggt
    42061 atagccaatc tggtttgtcc gaaacttaaa gccaatgggc agcgtaaggt attgcgattt
    42121 gaatttgata ttgtcgccaa tggcgatgtt gtaggtgcca tcgatagttt tcagggtata
    42181 ggtggattgc ataagcctga tgttcccgcc aagggtgtta accgagagcc ctgaggaaaa
    42241 ggcgtaccgt ttggcaaaga atttatcggc tgtaaagcca atgtttaagc ccacaacagg
    42301 gccgttggga tcgtaacgtt tggtatcgga gttgaaccac gatagctgcg ggtcagcaaa
    42361 tactccaaag ctgtattgct gggcatttgc cagttgatta ccaagcagaa ttaaaccgat
    42421 taagagtgta aggctattca acttcattgt ttcatcattt tttgtcttac cttgcaaagg
    42481 taaccattat tttaaagcga cttttcggat tgctgttcca aaaagtaaaa ccatatttca
    42541 ctttagctct aaatgtaacg ttgattacat tgcctaagta ttaactttgt gacctaaatt
    42601 atgctaaaac aatgattctg tttccaaatg ccaaggttaa tctgggttta aacatagtgc
    42661 gtaagcgccc cgatggctat cacgatattg aaactctgtt ttaccctgta aagggattat
    42721 gcgatattct tgaggtagtg gcaaccgatg gcgaggggaa caccattgaa ttcacccaaa
    42781 ccggaatagg cattgattgc gataccgaga acaacctgtg tgttaaggct taccgccttc
    42841 ttgcgcagcg ttacggaatg ccccatgtga ggatgcacct gcataagctc atacccattg
    42901 gtgcgggttt gggtggcggc tcggccgatg ccgcatttgc tttgagagcc ttgaatacct
    42961 ttctggacaa gccgcttagc catgaaaatc ttttatcgat gactttggag ctaggtagcg
    43021 attgcccgtt ctttgtgcat aacacgcccg ctattggcag gggccgcggc gagcagcttg
    43081 agcccataag cattaaccta actggttact ggattttgct ggttaatccg ggtatccatg
    43141 taagcacaag ggaggcctat gagggtagca atcccaaacc gtggaaaaac cccataggca
    43201 acattgttaa cctaccggta agcgattggc ccaacaaatt agttaacgat tttgagaaaa
    43261 ccgttttccc aaagtatccg ttaattgaaa gggtaaagga agaactttac agcatgggag
    43321 cggtttacgc atccatgtca ggcagtggct ctacagtgtt cggaatattt aaaagcaaac
    43381 ctgtaattac agatgcattt aaaggttatt ttacgcatac atcagaaatg taggttaagc
    43441 ggattgggtg atgattggta gaaaaatgtt tttttattgg atacagtgag tcgatttaat
    43501 gggttatatg ctcagtgcaa tttctcgaca acagggtata catactttag actattgcgg
    43561 ggctaaaatg tttctcctaa ttgtctgaac atttctattg tgtttacata cgaaacattc
    43621 aaagcaatac aagcatcagg tattttcact ttattctgtc tgttgggttc acttacttct
    43681 tgtgtaacaa caaacctgtt agcattgtca gcaaggcaat aagccacaag aaaagcatct
    43741 gcttcgtccg cattcagaaa ttcatttaat gcattcggta aataatgtcc acttctcgac
    43801 attgcccaag ctgtcacctg tgcataagca gtcattacaa ctgatgtgtc tttgaaaaag
    43861 tcttcgggca aattatttct gcaccattct tcgagtgcat cgtttctatc gtaaagctct
    43921 ttttttacct tgtcaatact gataaccctg ccttcttctg ccaattgtcg aaccttgttc
    43981 caaaatcccg cagcaacgtc aatcggatag tgaaaccgat gagcctgaat aaaaaagttg
    44041 ctatctaaga catatactgg cattcgggct acaagtgttt agagaagaaa ttttcaaacg
    44101 tgtcgccctt taagcccgta agtttatagg catcacgata taataattta ccagatttta
    44161 cagcctgatt aacatgactg gcaaatgtta tactgattct tttttttgtc gttgcgtaaa
    44221 agtcgccacc agaaccttga ctttcttttt tggcaaattc ccgattggaa tactcttcat
    44281 agaaatcaaa aaactgtggt ctgctgattt tgcctgtgtc tagagccctg cgtgcaataa
    44341 ctatttcact cactttaaaa taacgggaag cataagcgaa gttgttcggt ttgtgattcc
    44401 atacttcatc aaactcttgt gcagggacta aaaattcagc agcaacttta tcgcaaagaa
    44461 tttcaatcgg gtcgttggct ggttgtaacc ttctgaaatc gaaacctgca ctgtgacctg
    44521 tccaaatatg tgctaactca tgcacaatgg taaacatctg agcagatttg aaatcggaat
    44581 tgtttacgaa catgaaagga gcatattcat ccaccaaaac aaaacctctg cactcgtcaa
    44641 caggaatggg tcggtgggta ttgttttcaa ctatgccgtt gaaaatggta atgattcctt
    44701 tgtcttcaat atgcaaaacc aaatggtctt gggcatcttg ccaagttctg aattgacttg
    44761 cccaattttc agttagttcc agggtttggc gaatatctgc aactatagca tttacattat
    44821 tgctgtttct aaactttcct acaaatggta aaggatgaaa atcattatct tttaaatagt
    44881 ttcttaacca atcctgcctt tgctgtaaga gcaaaatggt atcaaacaca ttgatgctga
    44941 tacggtctgc ttggtttccg ttggtgcgga aatacggaat tggcagtttt tcttgtggtg
    45001 gctgtggcaa aaacagataa ccaaagggca ggtaaacttt cttagaaaat tcctccaact
    45061 gtttcactgt gggtttcttt tgcccttcga gccactccaa tattttagga aattttacgg
    45121 caaatgtagg cacatcataa cccgcccggg taatagccca ggttagcatg ttggtattta
    45181 cattaatctc atttgccata ctgcaaagtt attcatttcc catattttac ctgcggctcg
    45241 gctgctattg ataattgttc ttttgct
//
LOCUS       DAOSAL010000013        44651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_632_length_44651_cov_48.084043, whole genome shotgun sequence.
ACCESSION   DAOSAL010000013 DAOSAL010000000
VERSION     DAOSAL010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 44651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44651
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_632_length_44651_cov_48.084043"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            242..1618
                     /locus_tag="PK049_03355"
     CDS             242..1618
                     /locus_tag="PK049_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HOK60626.1"
                     /translation="MELPFAESYKIKMVEPIRRSTRQEREQWIKEAKYNLFNLPSNQV
                     YIDLLTDSGTGAMSDRQWSAIMLGDESYAGASSYFNLKNAIRNILGFDYIMPTHQGRA
                     AENVLFSVLVKEGDYIPGNSHFDTTKGHIEFRRAHAVDCTIDEAADTELIHPFKGNVD
                     PQKLDNFLKEHSQRVPFVIVTITNNTAGGQPVSMKNLREVREICNRYGKLLVFDSARF
                     AENAYFIKTREEGYAHKSIKEIVREMFSYADAMTMSSKKDAIVNIGGFIAMRDQELWR
                     QCSKFNIMFEGYVTYGGMSGRDMNALAVGLDEGTEFDYLETRIKQVEYLGNRLDEFGI
                     PYQKPAGGHAIFVDANKVLPNIPREQFRAQTLAIELYLEAGVRGVEIGTLLADRDPET
                     RENRYPKLELLRLAIPRRTYTNNHMDVVAVALKNVYDRRNQITKGLEIVWEAPIMRHF
                     TVELKPAE"
     gene            1842..3221
                     /locus_tag="PK049_03360"
     CDS             1842..3221
                     /locus_tag="PK049_03360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOK60627.1"
                     /translation="MKDFTTGNEAKHIFRFALPMLIGNIFQQLYNVVDSIIVGRYLGD
                     KALAAVGASFPIIFMVIALIIGIGIGSSVVISQYFGAKDFDRVKRAADTTYIFLFFAG
                     IIITIVGLLSGEYIFRLMRLPEEILPMAKTYLDIYMYGLVLMFGFNSVSSILRGVGDS
                     KTPLYFLIVSTFLNIFLDILFVLGFKWGIAGAAWATVIAQAFSFIIAVIYLERLRHML
                     RITFKRLVFDWEIFWQSVRIGLPTGIQQTLVALGGMALMGIVNTFGTEVIAGFSAASR
                     IDSLAVIPIMNFSASLSGFVGQNIGAGKVDRVKRGLWATIKMSVLFSVIITAIILIWG
                     DKMMVVFTTNPRVVDIGYHYLMVVSFFYVAFAVMFCINGLLRGAGAAVVPMYITLLSL
                     WIVRIPLAYLLSKTFGMNEAGVWWSIPIGWLMGAVASTIYYRYGSWKNKSVVKPVTTE
                     DDGVATDLV"
     gene            3290..3574
                     /locus_tag="PK049_03365"
     CDS             3290..3574
                     /locus_tag="PK049_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60628.1"
                     /translation="MASKRDLKKDIDYLVGEVISDCYTYMLINGEKKRDDAIAIIESI
                     LEKRNDLIHRIKHPAKKDDRKAVKAHYREIQKDALKAIDESFSKLSELTK"
     gene            3695..5659
                     /locus_tag="PK049_03370"
     CDS             3695..5659
                     /locus_tag="PK049_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOK60629.1"
                     /translation="MIQEFRQYFSDITLKGNIYLMLLWRFALLMLIFTLCRVLFYLFN
                     IDFFASVTTAGLLRMLAGGLWFDTSALIYTNILYIVLFLLPFTFRYNEWYQRVLKYLF
                     VVTNSVAIGANCADFIYFRFTMRRTTATVFSEFKHETNFGSLIPKFIADYWYVFLIWI
                     ALTALLVLLYGRVRPINKGKGFRFHLVYGLNGLVLLLVFATLMVGGMRGGFRHSTRPI
                     TLSNAGQYINEPLEAAIVLNTPFAIYRTLGKKSLVKVDYYKNPKELDEVYSPLHKPQV
                     TDSLKRMNVVIFILECFGREYIGAYNKHLKEKDGYTGFTPFLDSLIGHSLWFTHSYGN
                     GRKSIDGMPSVLASIPMFVEPYFLTSYSTNKINSIASALRAEGYHTAFFHGAPNGSMG
                     FQAFANVAGFEEYYGMSEYPDPSHFDGMWGVWDEEFFQFFAQTLNKFPQPFCAALFSV
                     SSHHPFKVPERYEGVFPKGTLPIHQCIAYTDYSLKRFFETVSRMDWYPNTLFVITADH
                     PNQTQFPEYQTSVGAFAVPLIFYRPDNSLAGQKDEFAQQIDIMPSVLGYLHYSKPFIA
                     FGRDVFDSTSKPFVVNYINNCYQYYSDDYVLIFDGKKTLGLYNIKDDTMLTNNLQGQL
                     PDRVNEMESKLKAIVQQYNSRMIDDNLVVK"
     gene            complement(5663..6094)
                     /locus_tag="PK049_03375"
     CDS             complement(5663..6094)
                     /locus_tag="PK049_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60630.1"
                     /translation="MQTKIIHILLFSILVFTNTACDDNIDDIIPDVPVNIEFNLKEPQ
                     YFNLTSVMGAVKIDGYGYRNNGVIVFRLSENEILAFDATCPKHIDINASVNPDSNGSG
                     TATCPHCGTIYYLVNYGYPKEGYPLKRYQVLKSGDYVRVYN"
     gene            6115..6495
                     /locus_tag="PK049_03380"
     CDS             6115..6495
                     /locus_tag="PK049_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60631.1"
                     /translation="MDKGIKKILFALIALFVLVGCGKQLAPSPVPATPGRSKWQVKRE
                     EYRRAWVAYWHDRKTERLTRGAKAKSVKREREAEKAEKKLAERHLKKQPPEVQERMKA
                     SLKEAEKNRPKRTLRQRLGFWKYK"
     gene            6509..7120
                     /locus_tag="PK049_03385"
     CDS             6509..7120
                     /locus_tag="PK049_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60632.1"
                     /translation="MDAGDFIYILIAIALAILNAFANSKKKKAAEQKRQAQTSAPADD
                     DLITKKLQELLGQEVIVENEEQPLESTVVNEEEKYIYEYSSKPEVETLDRSVEFEKEP
                     IDKPLTEEELYPIQPQKPIDSSTPIEYTPIDIAESKVEGPIGDYSYQDSFNESMHEVD
                     IDASDAEELESVKKVEEEKEPLLADFDPLKAVVYAEIIRPKYF"
     gene            7229..7702
                     /gene="greA"
                     /locus_tag="PK049_03390"
     CDS             7229..7702
                     /gene="greA"
                     /locus_tag="PK049_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOK60633.1"
                     /translation="MSKVTYLTEEGLQKLRKELEQLKTVERPAISRMIAEARDKGDLS
                     ENAEYDAAKEAQGMLELRISKLEDLIANARIIDESKIDTTQVQILNKVKLKNLKTNAV
                     VEYTLVAESEANLKENKLSISTPIGKALIGKKVGDVVDVQVPSGLMKFEILGISL"
     gene            7714..8109
                     /locus_tag="PK049_03395"
     CDS             7714..8109
                     /locus_tag="PK049_03395"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOK60634.1"
                     /translation="MATIFSRIVSGEIPSYKVAEDERFYAFLDINPLAKGHTLVIPKV
                     EVDYLFDLDPETLAGLTLFAQRVAKAIERVVPCKRIGVAVLGLEVPHAHIHLVPLNKE
                     SDIDFHKPKLKLTPEEFIELAKSIAAEFK"
     gene            complement(8170..10002)
                     /locus_tag="PK049_03400"
     CDS             complement(8170..10002)
                     /locus_tag="PK049_03400"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HOK60635.1"
                     /translation="MRKHLSFSLRYFVFWVILFSTYRIAFLIYQFENLKSVKFNEFIN
                     IFIRGAWMDSSLAGYILLLTFLLLAIFFWVSPKSITRMYNALTILLLLVVNTVVISDM
                     ELYRNWGYRIDATPLLYLKTPKEAMASVKTPLLILFVVLLIGITYLTHLAYRKWVARE
                     LSQANKARWWYSPIYIFLAATMIIPIRGGFGIAPINPGKVYFSQNNFCNHAALNPVWN
                     MMYSVSKSSSMSKTYNVEIDKATAHQRFGELMHTDSTLYVLNTNQPNIIIILLESFGA
                     KLVGPLGGLPNVTPNLNRLASEGILFTRTTASGDRSDKGIIAVLSGFPAQPTQSVIKY
                     PTKSNKLPTISEVLYNQGYSTAFYYGGDPDFANIRSFLYHAKFQRLITQDDFPKSYRN
                     SKWGVHDEHVFNYLLTDIDSAKAPFFKMFFTLSSHEPFEIPAKPKFNGKTEVEQFLSS
                     AYYADSCLGDFIAKAQQREWFKNTLIVLIADHGHRHPANHPNWEPLKFGIPMVWLGGV
                     VERKPLVVTTTCSQTDLATTLLAQLKLPHDQFTLSKNILASKVTPFAYFAFNEGFGYV
                     NQTDTIVYDLVGKKYIKQTGKNISETRNDAWAFFNAYQEVFQGL"
     gene            10061..11011
                     /locus_tag="PK049_03405"
     CDS             10061..11011
                     /locus_tag="PK049_03405"
                     /inference="COORDINATES: protein motif:HMM:NF013292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HOK60636.1"
                     /translation="MRKKILVLLFIVGFVVSNSFPQTWSIAVHGGAGNMKPENFTKEQ
                     LDEYEREMKAALGIGVKILNSGGTSLDAVEQVVRYLEDCPLFNAGRGAVFTHEGRNEL
                     DAAIMDGKTLMAGSVAGVTDVKNPISLARKIMEDSPHVMLAGKGASEFAKVKGIEIVD
                     TSYFFTPQRWNDLQRTLSKEKMGTVGCVALDMHGNLAAATSTGGMTNKRWGRIGDAPI
                     IGAGTYANNSTCAVSATGHGEYFIRYTVAHDISALMEYQNLSLNAAVERVIMGKLLNS
                     GGRGGVIAVDRSGNVCMMMNTTGMFRAFARSDGSNGFAIF"
     gene            11112..11627
                     /locus_tag="PK049_03410"
     CDS             11112..11627
                     /locus_tag="PK049_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60637.1"
                     /translation="MALEVHVKSAESKGHVEIFLLHFVLMLIAMFVFLALIMLIGGSF
                     TWPNFWKFYITFIPLTVILPVIVAITTSVSYKRVNVHILPASKVNIEKLKDFMFSEDY
                     IIVDKDKTFIRFIRRKNLPRLLSLNFDKPSITIQNDEVIVNVLKRLSLVLLPQLTIGK
                     KFIIEPESSKE"
     gene            11624..12724
                     /locus_tag="PK049_03415"
     CDS             11624..12724
                     /locus_tag="PK049_03415"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOK60638.1"
                     /translation="MIKGFPYLLIDIPKCEQNIAFMVQKATKAKVLLRPHFKTHQSLA
                     VGELFRKQGVNRITVSSVAMAEYFAAGGWNDITIAFPFVPQMAQRVNALALGIDLNVT
                     FSSPQNLLATIDRVESKVGVFVEVDVGHSRSGVAVDNTRELALMVNLISSKPNLVFKG
                     FLTHAGHSYSARGREEVDKLHSEALSKLAKLRSFWVDQFPDLTISYGDTPTCSVSDEF
                     WGIGEIRPGNFVFYDVMQYQIGSCTTSQVAVALICPVVDVYPGKQKIIVHGGAIHLSK
                     DRVGIDGADCYGFVCRFDGKGWSNPIPNAMVTSLSQEHGVISYSGNDLDGIKPGDLLA
                     ILPVHSCLAVDCMGQTFDSEGNTYDVLPKNKR"
     gene            12724..14058
                     /locus_tag="PK049_03420"
     CDS             12724..14058
                     /locus_tag="PK049_03420"
                     /inference="COORDINATES: protein motif:HMM:NF021021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HOK60639.1"
                     /translation="MKRLTQFLLFSVISTLTTAQSTKSNSVFDKYFENKTMRVDYIHG
                     GNSFSEDFKIFGIKNDGEWGGRVRVMDDPYILGLYAFDIIDAASGKVLYTQGFCSVFG
                     EWQTTAEAGKLNKNFEESVRFPWPKDIFKLVMRKRDSTNTFQVIWSETINPSMYADAN
                     HAYPAAKVQYFVKSGNPKEKVDIVVLADGYTVADSAEFRKDVERFTEAFFSVEPFKSR
                     KTDFNVAAVYSTSPTSGIRLQKHNYYPANVLGCTYYTFGTERYVLTEREWTVRDFASA
                     VPYDFLVILLNSNKYGGGGIYNLYITASAKSSSSGYVMVHEMGHHIAGLADEYYTSDV
                     AYQVSAPKYEPWEFNITALLNPASLKWKNMVEQNTPIPTPWQKENYERTGKLRIADEV
                     YSGKVGAFEGAGYLSKGLYRPEVDCIMFSHSLTFCRVCQEGLNRVLDMYVVR"
     gene            complement(14051..16237)
                     /locus_tag="PK049_03425"
     CDS             complement(14051..16237)
                     /locus_tag="PK049_03425"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOK60640.1"
                     /translation="MFKPITFKAFLLLVVFGIPTLRVNAKPEPLDNIDSLKYFENLTR
                     QYERNGDKVKLVETLIAKSQYLFNKKNLDNALIEANQALIVSLDMGREQYLEKAYYLL
                     SQIHIEKGLLYESIDYLYPGYRYSIATRDSLKIGWYLLSISKVEGELGRLNNAIENNL
                     TAINFFKSRNDSLSLAKIYTSQGIIHTEFGNFVTAQSYLENAIAILKVVVDSTYLGIT
                     YRALANNSLRQSNNLMADKHLKKSETYLKNNPKELARTQTVGAELLINQLKPNSAIPL
                     LQSILNTQNEINDRYGQLYPLLLLGKAHARVGNYSESKKYLLQCLKQSESLSITNISR
                     LAYKELSQLEELQGNKTLGYQYLRKYISLTDSLFNNQIISEANRLENISALRQKEKEI
                     QRQRENLLLSQANLNKSRLRQLFLVGFIAILIVLVVVSYLAFRNKQKANLLLAQQKEK
                     IEKQKNMLEQRSRDITDSLNYARRIQKAILQTSEQPTDFFDDSFLIFLPKELVSGDFY
                     WFKRFNNQMLFAVADCTGHGAPGAFMSIIGMFGLNQIVSELNETNPGEVLQNLNELFH
                     KSFEQREGSEIFDGMDIAFCNYNPETRELRYSGANIYLNIVRKSNSQKVSNIILNQNE
                     THTLYQVKCERQSIGYVTEKVSFVTHSIQLEKDDIIYLYTDGFTDQFGGPNGKKFRLV
                     DFRNILCSIASYPMAKQKDFLLEIFSEWKGDNVQVDDVTVMGIRIT"
     gene            complement(16305..>17332)
                     /locus_tag="PK049_03430"
     CDS             complement(16305..>17332)
                     /locus_tag="PK049_03430"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOK60641.1"
                     /translation="ITRETISPEKKKIYADYITSNGRLLLTLIDDIIELSKIEAGIYS
                     IRRQPVELDSLFNELHQFAINEKKRQKQNILIARDIGNNSGITHILADGERLKQVLLN
                     LLSNAIKFTSQGSVTMGYRIPNNATIEFFVSDTGIGIPKSLQEKIFERFRQADETAQR
                     LYGGAGLGLAISKRLVELMGGRIWVESEPNKGSRFSFTLPLIIPNTKNISRINFITKN
                     DYTKIKGKTILIVEDNEANVNYLSDVVIASGAKSLTAMSGSQAIDLLNREKIDAVLLD
                     IQLPDTNGLELISRIKEKIPNVPILVQSAYTPAEYIDKAIARGAYGYLTKPIKVNELL
                     DLLSELF"
     assembly_gap    17333..17432
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17433..18106)
                     /locus_tag="PK049_03435"
     CDS             complement(<17433..18106)
                     /locus_tag="PK049_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60642.1"
                     /translation="MTKNNKNHIDINDLPIAVLLVKRDGTVLNYSPSVLLLTDKPIPD
                     GCKIESLFPNLPFKTSKKERTYTFEHSTHGKQHTYRANVKPVEWNIFLVWIENITLSN
                     KVERINRILVKLSAIGQETTSPEDFYRLMQEELNTLFDAMNIYLVLFDKSRQRLSLAY
                     VSDTHPINTDYPSGNTFALWVAHRGQGVILNRQQMNRLRSKHNLTFYGPPALCWMAVP
                     LKTKSDT"
     gene            18302..19423
                     /locus_tag="PK049_03440"
     CDS             18302..19423
                     /locus_tag="PK049_03440"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOK60643.1"
                     /translation="MHKILYVLLFAIISISCGEGGKPSSNSIHGNGYAKHAKGFDIEI
                     DGSVTKVWVNNPFEPTANGLVYTLYADSMRSIDPNSIQIPVKRVVCMSSTHVGFIGAL
                     GANSAIVGISGTKYVNNPKLWERVVNGQIAEVGYDQTLNYELLTRLKPDVIFVYGVSS
                     ESLSFIDKLKELGLKMVMVSDYLETSPLGRAEWIKFFGVFFGKKELADSIFSEVEVNY
                     AKLTQSVEQVDTKPKVFLNLPFRDIWYFPGVDNYFVKLINDAGGSYIFPELTGSQSHP
                     VSTEVAYKAGLSCDIWLNPGTANSLSDITNSDNRFSMFKSFGNARVYNNNKRMGQGGG
                     NDFWESGVVHPEQILSDMIKIFHPELMPTDTLYFYRRLE"
     gene            19453..19773
                     /locus_tag="PK049_03445"
     CDS             19453..19773
                     /locus_tag="PK049_03445"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HOK60644.1"
                     /translation="MKFGVIIETKEPEKAWNAFRFAVASLGKGHEVKVFLMGEAVECQ
                     GLTHNKFDVDAQMRKFTAEGGTILACGTCLKSRQMDGTELCPISTMADCIQMVEWADR
                     VITF"
     gene            19833..20813
                     /locus_tag="PK049_03450"
     CDS             19833..20813
                     /locus_tag="PK049_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008782459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOK60645.1"
                     /translation="MVVLLLLALLSLLLGSVAIPLTDIKAYFFGGSFTDEVNLSIIQN
                     FRMPKVVSALLAGSALAVSGLQMQTVFRNPLAGPYVLGISSGASLGVALVLMGSSAFG
                     LSFWGSQVGTVVAALVGSFLVLMLILAVSIRVRDIMTLLILGMMFGTAVSAVTSLLQY
                     FGSETSLKNFVIWTMGSLGGVGRQQLWLYAALVLLGLALAWAYVRPLNVLGIGDTYAL
                     SLGFNVKQTRFLIFLSTSLLAGTATAFNGPIAFVGIAVPHLARMVFRTSNHAILVPAS
                     ALAGAATLLLCDIIAQLPGSAAVLPLNSVASLMGIPVVIYIVLKNKRVVS"
     gene            20813..21832
                     /locus_tag="PK049_03455"
     CDS             20813..21832
                     /locus_tag="PK049_03455"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK60646.1"
                     /translation="MNGIVIDIKGLVSGYRNGVSSTYRYPAFNAVALRGELIALIGRN
                     GVGKSTLLRTLARLQPSLDGNVFVKGVNFQQISGSEFAQLVSFIPSEPAHASHTTVYE
                     FVSMARYPYHGWFETLNDNDYKLVDDAISAVGMQNFRDRFIDNLSDGERQRVMIAFAI
                     AQDTPIIMMDEPTAFLDLPNKFEVMRLLREQAQKGKTIIISTHDLQTAFGLVDTIWLM
                     LADGVRVGAPEDIVLSGNLNRLMDDTPVLFDLKSGQFVYKHQTRLVACVLGGSPNIKK
                     WTSHALRRMGYEVEFSPIDDASTYVQIQEHEGKVQWILGRDDRNPTFDSIRNLCIYIK
                     GTTLL"
     gene            complement(21827..22597)
                     /locus_tag="PK049_03460"
     CDS             complement(21827..22597)
                     /locus_tag="PK049_03460"
                     /inference="COORDINATES: protein motif:HMM:NF007757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOK60647.1"
                     /translation="MKIAVIQSKLYWELPDNNFEQFEQKLAGIEPGTHLVLLPEMFTT
                     GFSMSPASLAERHPGRTVEWMKSMAKKHGFALAGSYIAETDGKYFNRMVFAYPNGTIE
                     TYDKRHLFRMANEDAHYTSGTQRVIVTHEGWRILLAVCYDLRFPVWLRNWNDYDLMIL
                     VANFPERRRYAWNSLLVARAIENQVYVAACNRVGTDGNQINHTGDSQIIDPMGQIIAQ
                     AKPNTEEVIYSSIDKDYLHSVRSSFPVHLDADSFEIKL"
     gene            complement(22572..23426)
                     /locus_tag="PK049_03465"
     CDS             complement(22572..23426)
                     /locus_tag="PK049_03465"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOK60648.1"
                     /translation="MNFPPDNQQSFFFYNGKILPLNKFSVALTEGENIIYEVIRVKNS
                     TPIFLNDHLNRFMYSAQMMGYNINLDEITLGIRELLKANPVDQRNFRLMYYSNENGAT
                     LLIYFIPSRYPTADEISTGVRLETLNAERHNPTVKFENKAIRAIANEILETHRCYEVL
                     LVDHDGFITEGSRSNVFFIKDNELITAPNSKVLGGITRQKVIEICNTLQININFRSLG
                     ANEIKQTTGCFITGTSPSVLPVKQIDNHIYHEIPDLVRDISAHYETLVEQCIINWRTE
                     NEDSSNSK"
     gene            complement(23510..24658)
                     /locus_tag="PK049_03470"
     CDS             complement(23510..24658)
                     /locus_tag="PK049_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60649.1"
                     /translation="MKKIATYLGIAIACIIGIVIALHLAWYFQKSKPLNIYILDKTVT
                     QTDRIEHKSLVWLLNHFRIVGPDGKSYNHKSDYWGFFPIDLNQQLFDIKSIRLNEVDA
                     YAAVYDAAYYTDCYGVYSYEWYKTETKAPNTSKVYGGLNQNDYLLLKRMKELNKLIIG
                     EYNMFSSPTNALIRTKTEELFNVSWTGWAGKYCPNLGAINNGKGPAEWMVKLYEKQHQ
                     KPWPSKGSGIILISNDGLIDVLEEGSDLLNPRVKLIAEPTMPQWQNNSKETEFYGWFD
                     IIMAGIGSEVHATFKLETTPAGEEKLKSFGLTNQFPAVVKGYSDKFCYYFAGDFAENS
                     VSIATSKLKGGKTLNSILHLNSPRNAFFNNFYLPLINNILDIYTSREQ"
     gene            24707..24850
                     /locus_tag="PK049_03475"
     CDS             24707..24850
                     /locus_tag="PK049_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60650.1"
                     /translation="MKKITSEGDKFVKRESLTVNREPLKVKREKLKVKSLALSGNESE
                     YSE"
     gene            complement(25364..25672)
                     /locus_tag="PK049_03480"
     CDS             complement(25364..25672)
                     /locus_tag="PK049_03480"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOK60651.1"
                     /translation="MIKPETLQSAPKVPFNLDGRIMFSSSKVEIVHLTLKPGEVIPAH
                     ANPFGVVFYLLDGELELTVNNESALLTPNSATFVEINESRSLTNKSTNTARVLVVKIF
                     "
     gene            complement(25685..26470)
                     /gene="ccsA"
                     /locus_tag="PK049_03485"
     CDS             complement(25685..26470)
                     /gene="ccsA"
                     /locus_tag="PK049_03485"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOK60652.1"
                     /translation="MVWNYFVPVALTVILLWLVSVYFNHKESAKGKLILWYSGVTASA
                     LLGVFIILYWFDIKRPPMRTMAETRIWYSFLVSLVSLSVFKAYRSKNILTIGLIMASV
                     FLVVDIIHPEYQGRFLMPALQSPWFIPHVVSYMLAYAVLASASLSVAWTSFSKGSVHD
                     ELQQQMRLVYPGFALLTLGMLLGAYWAKFAWGDYWAWDPKEVWALITWFFYLLVIHIH
                     RYLPTKRKLLARLMIVALGVLLITWLGIKYLPSASQSIHVYGG"
     gene            complement(26475..27692)
                     /locus_tag="PK049_03490"
     CDS             complement(26475..27692)
                     /locus_tag="PK049_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60653.1"
                     /translation="MGNQFKKKPLWLWPWGFLEGFIVSVGLWVLAFFLELFVPLSLFP
                     KLGFPTNAIVLVTILSLLILLKIIFPKSKFFNWLASVKSAIPSISLFLFSIILMGIIP
                     QIGHGMGIPGALFNLLGSWLFLFPLIFMLITLGAAVVNRVFPITLKNIIFTLNHAGLW
                     VCLSAGLVGSSDKIEATVQVNQNEIAWYGTDKNGSVIELPIAIELQKFEAQFYQPRLA
                     LMGSKTFFPESEYDLNSNPSIKIDNLTIQVMQYLPKAFATDSTFIDARGVPFTGPAAM
                     IQVFDSNGQTVTMGWISSLTHVTAEKTLPLPDGRELRLLPAEAKYFGSYAKIYSKKSS
                     SVNQCLIEVNKPFRVDGWWIYQYSYDNLAGPDSTYSSFKVIYDPWLPLIYIGFAMMLL
                     GGLGLVLINDSKK"
     gene            complement(27704..29215)
                     /gene="nrfA"
                     /locus_tag="PK049_03495"
     CDS             complement(27704..29215)
                     /gene="nrfA"
                     /locus_tag="PK049_03495"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase"
                     /protein_id="HOK60654.1"
                     /translation="MKTPLSEKIAKNPWIGWALFLGTMIVVFVLGLLAASIVQRRTEA
                     NLMNTPRYKIDEFEPRNEAWGMAFPREYQTYSQMSDTLFRSKYGGSAFRDLLDEDPRL
                     VILFAGYAFSKDFNQARGHVYSVADVHNTLRTGTPANENEGPQNSSCWACKSPDVARV
                     MAKMSPADFYKTKWSAMLSEIVNPIGCANCHNPSDMTLRLFQIPLKEAYERMGSNVEL
                     LPLNKMRTMVCAQCHVEYYFKGDGKYVTFPWDKGLNVDDIEKYYDEYAFADWIHPLSK
                     TPIIKAQHPDFELFQHSIHYQRGVSCADCHMPYVTEGSQKITDHKVQSPLNNMEASCM
                     VCHKQTKEELIKNVYDRQDRVYTQKIVLEDLLVKAHIEAKFAWDNGANEKMMEPVLKL
                     IRQAQWRWDFVAASHGASFHAPLESMRIIADGIEKASEARLQLSRVLATLGHNEPVPM
                     PDLSSKEKAQAYVGIDLKKLKGEKEEWKKNVLPKWLQLAKEREQNMPLPERIM"
     gene            complement(29235..29822)
                     /gene="nrfH"
                     /locus_tag="PK049_03500"
     CDS             complement(29235..29822)
                     /gene="nrfH"
                     /locus_tag="PK049_03500"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF015237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HOK60655.1"
                     /translation="MFDWAKQILKRFQPPPNWRVPVIILAGIFTGTGLFVFYTSRAWS
                     YVSDDPATCVNCHIMRTQYVTWHHSSHREHATCNDCHVPHDNIVRKYYFKANDGLRHA
                     TIFTLNTYPQTIYMHKPGQRVVQENCIRCHGNLTAMVKANVDLDAILAGNGKRCWDCH
                     RDVPHGTVKSLSATPYGTAPVPKTDTPNWLKKLIK"
     gene            complement(29863..31368)
                     /locus_tag="PK049_03505"
     CDS             complement(29863..31368)
                     /locus_tag="PK049_03505"
                     /inference="COORDINATES: protein motif:HMM:NF015863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HOK60656.1"
                     /translation="MSFYSTLRKYHKWPALVFTLFFLLFAISGVLMNHRQTIAHIDIN
                     RKYLPKNYQLKNWNLASVKGARTINSDSIILYGGVGIWLTKSNFENWKPLMDGIPEGS
                     DYRKIFDLEVTPNGILAATRFGLYWLGKGENHWVKLHLPQSDEFCTSIEVVESSIYIT
                     TRNSIYYSSAQTLQFTPIDLKAPLGSKKEISTFRLFWNIHSGEIFGIPGRLLADLVAL
                     VMIFLSITGIIYFLSPKIIKRYHEQFRFHKLKRTTRFSYKWHLKVGALATLLLVISGL
                     TGTFLRPPFLLTIANSQIVLNNQKVSNSYYWNDKLRDIRFDSARKKFILATSDGIYHC
                     TDFSEPLTKFPNQPPVSVMGINVLEPLDSVFYLVGSFSGLFRWNPDTGETFNYITGNL
                     HRRENGLRKPLGENVVSGYAHISGLEYIFDYDKGMVALHHSEPAIPMPSTVADAFKFP
                     LWNLAQEIHTGRIFSFMLGDFYILVVPLTGIFLVVVVLSGFMMWYKRKYLN"
     gene            complement(31402..31923)
                     /locus_tag="PK049_03510"
     CDS             complement(31402..31923)
                     /locus_tag="PK049_03510"
                     /inference="COORDINATES: protein motif:HMM:NF020553.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HOK60657.1"
                     /translation="MDENKLIITPKTKVLQLIEAYPQLEEVLIGYVPAFEKLKNPVLR
                     KTVARVATLQQAAAIGNVKVEELINVLRKAVGQDLLEGADEMEFTTQKPQWFDSNKVD
                     SEIDVRVMLQAGEQPVNQIMADLTDLSSDKIYKVVAPFITAPLIDKASSLGIKHWVEK
                     IDDEHFNIYFYKP"
     gene            complement(31926..33158)
                     /locus_tag="PK049_03515"
     CDS             complement(31926..33158)
                     /locus_tag="PK049_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HOK60658.1"
                     /translation="MSELINNSENRKKKLKELILRLHKGDSEQEVRQELIQSLTNIPY
                     GEVVEVEQELISEGLPEQEVLKLCDVHSAVLKGNIDLTTVKKIPDGHPVDVFIKENKE
                     LNQLCQSIEQSLMELVSSEAVDIPKLVLKLRGQFNALFDVDKHYQRKEYLLFPFLEKQ
                     GITGPPKVMWGKHDEIRELIKGSIELLQTEGISRDELIASSEIVLRPAIKGVMEMTTK
                     EEEILFPMALDKLTEADWYEIHKQQLEIGFCLYDPPTKWKPSWVEGSELQELNKTAEN
                     IQLPTGSFSVEELLAILNTLPVDITFVDKNDKVKYFSQSPERIFQRNRAILNRDVRLC
                     HPPASAHIVDKIIEDFKSGKASRAPFWINIGGNLIHIEYFALRNEKGEYLGVLEVSHN
                     VSVYRKLEGEQRILSYSK"
     gene            complement(33220..33552)
                     /locus_tag="PK049_03520"
     CDS             complement(33220..33552)
                     /locus_tag="PK049_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016269642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOK60659.1"
                     /translation="MNLTNEFPKGQKFNFMNEVEYSNGGIVSKNVLKRPTGNISLFAF
                     DKGEGLSEHTAPFDAVVQVIEGKATIIIGGTPHQLVAGETIIMPANIPHALQAEERFK
                     MVLTMIKE"
     gene            complement(33626..35272)
                     /gene="hcp"
                     /locus_tag="PK049_03525"
                     /gene_synonym="priS"
     CDS             complement(33626..35272)
                     /gene="hcp"
                     /locus_tag="PK049_03525"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOK60660.1"
                     /translation="MSMFCYQCQEAAKGVGCTIKGVCGKNDDLSNMMDLLLFVTKGIC
                     VYSTALREQKGYESPMVNKFVFDSLFSTITNANFDKAAITKRVEKAIEIRNMLREEAQ
                     KKGVKVDTTFEGCTWEGKPEEFDAKAATVGVLNETNEDIRSLKELITYGVKGMAAYAE
                     HAYNLGFEDTTVYAFMQKAMADITKNLSVDELVTLTLETGKYGVQTMALLDKANTTSF
                     GNPEITKVNLGVRNNPGILISGHDLKDMEELLAQTEGTGVDVYTHSEMLPANYYPAFK
                     KYKHFVGNYGSSWWHQREDFESFNGPILFTTNCIVPPLANANYKDRIYTTGAAGLEGA
                     KHIPDRENGKPKDFSAIIEHAKRCKPPVEIEKGEIIGGFAHEQVFALADKVIDAVKSG
                     AIRKFIVMAGCDGRMKDRNYYTEFAEKLPKDTVILTAGCAKYRYNKLPLGDIGGIPRV
                     LDAGQCNDSYSLAVIALKLKEVFQLNDINELPIAYNIAWYEQKNVIVLLALLYLGVKN
                     IHLGPTLPAFLSPNVAKVLVENFGIGGITTVDEDIKLMVG"
     gene            complement(35342..36253)
                     /locus_tag="PK049_03530"
     CDS             complement(35342..36253)
                     /locus_tag="PK049_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOK60661.1"
                     /translation="MKRDIITIDRDKCNGCGLCVSGCHEGALQLIDGKAVLISELMCD
                     GLGACIGECPEGAITIEHREAEPYDEVHTISKMVANGKNTIIAHLKHLKDYNEKEYLR
                     QGVEWLLANRDKLTFSVDEVLQAVHNHTKDACGCGTSEPKVKPQPMHHHGGGCPGSAT
                     RSFAQAIAGAEGMVSGKSELTHWPVQLHLINPHSGHFRGSNLLLAADCVAFSLGNFHS
                     QHLKGKTLAIACPKLDSNKESYVEKITALIDDAQVDTITVMKMEVPCCGGLVQLARMA
                     MEQAKRKVPIKVITVGIQGEILESNWV"
     gene            complement(36619..37827)
                     /locus_tag="PK049_03535"
     CDS             complement(36619..37827)
                     /locus_tag="PK049_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008761764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOK60662.1"
                     /translation="MKKYIRREMYEAKIRPFIGKDLIKVIVGQRRTGKSYFLFQVMDL
                     VQQLNPDIQILYINKEDLAFDTIKTYADLVSFAESNVTKGKKAAIFIDEIQDIEDFEK
                     ALRHFHTTGMWDIYCTGSNAKLLSGELSTYLTGRYVEITMYSLSYPEFLVFNRLENTP
                     ENFNKYLRFGGLPYLVNLTLNENVVFEYLRNIYTTILYKDIITRYNIRNAAFLERLVI
                     FIADNIGNVFSAKKITDYLKSQNIKSSNNIVLDYLNYLQNAYLIAKISRNDLKGKRIL
                     EIGEKYYFHDTGLRHAIISYKPGDINQLLENIVLLHMLIHGYSVTIGKLGDKEIDFIC
                     DKNSNRIYIQVTLSLADENVRTREYGNLLETGDNFRKIVVTADEYALDTYKGIETINI
                     REFLTGTKIP"
     gene            complement(38543..39955)
                     /locus_tag="PK049_03540"
     CDS             complement(38543..39955)
                     /locus_tag="PK049_03540"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-like domain-containing protein"
                     /protein_id="HOK60663.1"
                     /translation="MKKFFLITLTLSLMVFASAFAQKNGYRIEVKIDGLTQGDLLLGY
                     HFGDKKYVKDTATVNSKGFAVFEGDTLLPGGIYLVILPEKNYFEVLVDKNQKFSVATS
                     KDNLLENLKFTNSSENEGFIKYQRFMIDMQKQSKELSEQLKESLSDSLKANAIRDKMN
                     ELNNKVLAYWDEVEANYKGTLLAAIIKAMRNVEAPKHNIAKDTPKYDSLVWAHNYNYN
                     RYHFFDNIDFSDARLLRTPILESKINVYFDRVLLPMPDSIIPAAIELIEKAKANKEMF
                     QYILSTLTNKFQTSERMGMDGVFVAVAEKYYLGGQAWWADQKLLDKIAERVKAIKPNI
                     IGNIAPDLWLPNPNGKYYRLSDVNAKVTVAYFWDPDCSHCKKVTPELKKVYEKYKGKG
                     FEVFAVYTQGDQPKWMEYIKANELTWINVWDPTFSSNFRNLYDIYATPVIYVLDKNKK
                     ILAKRISVETLEKILEEELK"
     gene            40085..42097
                     /locus_tag="PK049_03545"
     CDS             40085..42097
                     /locus_tag="PK049_03545"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOK60664.1"
                     /translation="MDTWKDARPNRLVREVSPYLLQHAYNPVDWFPWGEEAFAKAKAE
                     NKLLLISIGYSSCHWCHVMEHESFSDEKVAKTMNERYVCVKVDREERPDVDQIYMNAV
                     QIITRRGGWPLNCFALPDGRPVYGGTYFPKENWLSILESLDETWKNEPQRIYEVADEL
                     AEGIANTEIIRVKADVSNVNLNVVLAENLNSFRNQLDFKLGGTLGAPKFPMPGLLKYL
                     LITGQHSQRKDLLAFVDTTLERMANGGIYDHVGGGFFRYSVDEKWFVPHFEKMLYDNA
                     QLIEVYSLAYRLNPNPLYRRVVEETITFVERQLLSSTGGFFSALDADTAGLEGGYYTW
                     GKSEVEQILGYDSELFCVAYGITPTGNWESTNILFRSLTNAQLSSLFGLPEGEIDYRL
                     KTSLIKLKNHRDFRVPPLVDDKIITSWNSLMVSALSTAYLTFGYDKYLQLALSSANYL
                     IDNHVNDDWTLSRITCKGRVYGTALLDDYAYTINALINLYQITNNTLWNRYAINLAQK
                     TKDIFFDTNSGMFYYTPASTELITRKMELMDGVMPSASAVMVSNLLALAEHDTSSEYG
                     TIALQMIANVGNHIKQGNVFVHTWALQLLSTILPVAEIKVNGDDLNAAEMLIASKLVY
                     PKIKFLQSTDIPNKYLLCIGNTCQSPTDNSENIVKWTNAISFEYYV"
     gene            42588..44327
                     /locus_tag="PK049_03550"
     CDS             42588..44327
                     /locus_tag="PK049_03550"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HOK60665.1"
                     /translation="MAYSFDSYRVEVLQRPFVRSDGFTTKQTAKIELFNSKGVLITTL
                     SLGIPEMADVYERIGKMEPVNLDYCYIQGFSTIACKRYLQIDKAQPLPVAQFTACNSF
                     FDSPVSIDLSLSISEKEFVIDESYVVTELFDFHLSNLLGSFRLTNCYIKAAKSNFTQV
                     RFEGEQISFKNTIFDVGVKDFQDTSFGSGEAIFTNVDFGDGDVSFINAGFNNSDVDFR
                     VCRFGTGKVDFHYARFGGGGVSFERVDFGSGRVDFRAAEFRAGRTSFNRASFHEGELN
                     FEGAETPKGKLTFKRAAFGNVTLIFDLYQGAKSEIVFERSLFSQNISFSEAQLGKLIF
                     SDCQFNSMVNLHVKQSDEINLAGCTIRDIVEFYTHGDPPRINSLNLTGVRLLGQIYID
                     WDANGVRSLIYSQGCTTVAEKAEQFRILKENFNALGKYDEEDAAYVEFKRCHMRALLL
                     SSNKQGKLRHLINRIIYWAKKLLFDYMGLFATSPQRVIFSILVVYVIYSLAYIAIMGL
                     GLGHIIATSASGDQLGIVARGFYFSVVTFFTIGYGDFAPSGIARFVAGTEGFMGVFLM
                     AYFTVAFVRKILR"
BASE COUNT        12031 a         9060 c         9904 g        13556 t
ORIGIN      
        1 taaaatcgtt ttcgatttgt aattttttta tttttgcatg agttgaaaat ctacgagagt
       61 gatatttaag gaaaacatat acctaaagtt tactgctaag tacgtgtttc tgaatataaa
      121 gaaccgcgat aggttctaat tttttccacc cagccatacc ggcatttaca tttttcgcct
      181 ttggtgaaaa catctgcttt tataaatcga tatcagttta acaattaaaa tatttattag
      241 tatggaacta ccatttgccg aatcgtacaa gattaaaatg gttgaaccca ttcgacgtag
      301 tacccgtcag gagcgggaac aatggataaa agaggcaaag tacaaccttt tcaatttgcc
      361 cagcaatcag gtttacatcg atttgctgac cgattcagga accggggcta tgagcgaccg
      421 ccagtggagc gccataatgc ttggcgatga gagctatgct ggggcttcct catacttcaa
      481 cctgaagaac gccatcagga acatccttgg ttttgactac ataatgccaa ctcaccaggg
      541 acgtgctgcc gagaatgttc ttttctcggt actagtgaaa gagggcgatt acatccctgg
      601 caactcccac ttcgatacca ctaagggtca tattgagttc cgccgtgccc acgctgtgga
      661 ttgcaccatc gacgaggctg ccgatactga actcattcac cctttcaagg gcaatgtcga
      721 tccccaaaag ctcgataatt ttcttaagga gcattcccaa agggttccat ttgttatagt
      781 aactattacc aacaatacag ctggcggtca gcccgtatca atgaaaaacc ttcgtgaggt
      841 tagggagata tgtaaccgtt acggcaagct tttagttttt gactccgctc gttttgccga
      901 gaatgcatac tttatcaaaa cccgagagga gggctacgcc cacaaatcca tcaaggagat
      961 tgtaagggaa atgttctcct acgccgacgc catgaccatg agcagtaaaa aggatgccat
     1021 tgtcaatatt ggtggtttca ttgctatgcg cgatcaggag ctttggcgtc agtgttcaaa
     1081 gttcaacatt atgtttgagg gttatgttac ctacggggga atgtcgggtc gcgatatgaa
     1141 tgctctggct gttggccttg acgagggaac cgagttcgat tacctggaaa cccgaatcaa
     1201 gcaggttgag taccttggta accgcctcga tgagtttggt attccctacc aaaaacctgc
     1261 cggtggtcat gccatctttg ttgatgccaa caaggtatta cccaatatcc cgcgcgaaca
     1321 gttcagagcg caaacacttg ccatagagct ttaccttgag gcaggtgttc gtggtgttga
     1381 gattggcacc ctacttgccg accgtgaccc cgaaacccgt gagaatcgct atcctaaact
     1441 tgaactcctt cggcttgcca ttccacgccg tacctatacc aacaaccata tggatgtggt
     1501 ggccgttgcc cttaaaaacg tttacgatag gcgaaaccaa ataaccaaag gacttgaaat
     1561 tgtttgggaa gctcctataa tgaggcactt taccgtggag cttaaacctg ccgagtaata
     1621 cctttattca tatttgcaag agccggatag taaagtccgg ctcttttgca attttatcaa
     1681 gtttttttca ttaaccttga tttattgaat tactaatcgt gcttaaaaac aatacctgtt
     1741 gcactaaaca ccaaaaacta aacacaaaac acaaaacaca aaacactacc tttgtagttc
     1801 tcattttttt tagcctactt tgcaatgcat ttcattaagc catgaaagac tttaccactg
     1861 gtaatgaggc caaacacatt tttcgatttg cactgccaat gctaattggc aatatttttc
     1921 agcagctata caatgttgta gatagtatta ttgtgggacg ctaccttggc gataaggcat
     1981 tggctgcagt tggggcttcg ttcccaatta ttttcatggt tattgcgctt attattggta
     2041 ttggcattgg ctcatcggtt gtaatttcgc agtactttgg tgctaaggat tttgataggg
     2101 ttaaacgggc agccgatacc acctatattt tcctcttttt tgcaggtatt attattacca
     2161 ttgtaggttt gcttagtggt gagtatatct ttaggttaat gcgcttaccc gaggagattt
     2221 tgcctatggc aaaaacctac ctcgatatct acatgtacgg gttggtgcta atgtttgggt
     2281 ttaactcagt atcgagcata ctacgaggcg ttggcgattc caaaacacca ctttacttcc
     2341 ttattgtttc aacatttcta aatatttttc tcgatatcct ttttgttctt gggtttaagt
     2401 ggggtattgc tggtgctgca tgggctactg taattgcaca agccttttca ttcattatcg
     2461 cagtaatata cttggaaagg ctaaggcaca tgcttcgcat tacctttaag cgattggttt
     2521 tcgactggga aatcttctgg caaagtgttc gaataggctt acccaccggc attcaacaaa
     2581 cccttgttgc attgggaggt atggcattaa tgggcatagt aaacacattt gggactgagg
     2641 ttattgccgg tttttcagca gccagccgaa tcgactcgct agcggtaatc cctataatga
     2701 acttctcagc atcgctttcg ggttttgtgg ggcagaacat tggtgctggt aaggttgata
     2761 gggtaaagcg tggcctatgg gctaccatca aaatgtcagt gctgttcagc gtaattatca
     2821 ctgcaatcat acttatttgg ggcgataaga tgatggtagt attcacaaca aacccaaggg
     2881 ttgttgatat tggttatcac taccttatgg tggtaagctt tttttatgtg gcatttgctg
     2941 tgatgttctg cataaatgga ttgctacgcg gggcaggggc tgcggttgtg cccatgtata
     3001 taacactact atcgctctgg attgtacgca tacctctggc atatctgcta agcaaaactt
     3061 ttgggatgaa cgaggctggg gtttggtggt caattccaat tggctggtta atgggtgcag
     3121 tagcttcaac tatttattac aggtatggaa gctggaaaaa taaatcggtt gttaagcccg
     3181 taaccactga agatgatggg gtggctaccg atttggtgta gcagattaca cagttggtga
     3241 taacacaaaa agtattacct ttgcaaactg ataaataaaa ctactgatta tggcaagcaa
     3301 gagagatctt aaaaaggata tcgattacct ggttggtgag gttattagcg attgctacac
     3361 ctacatgcta ataaatggcg aaaagaaacg cgatgatgca attgctatca tcgagtcgat
     3421 tcttgaaaag cgcaacgatc ttattcatcg tattaaacat cccgccaaaa aggatgatag
     3481 aaaagccgtt aaagcgcatt accgcgaaat tcagaaagat gccctaaagg ctattgatga
     3541 aagttttagc aaacttagcg agcttacaaa gtagtattcc aaaacaccac atatttcccg
     3601 gccctttgcc gggatttttt gttttatgtt ttcacagcac tgcggcataa aagaattctt
     3661 aattttgcag ttcaaccatc taattacata agccatgatt caggagttca ggcaatactt
     3721 ttccgatatc accctaaaag gtaacatata cctaatgctt ttgtggcgat ttgctctttt
     3781 aatgctgata ttcacccttt gccgggtact tttttacctg ttcaatatcg atttttttgc
     3841 cagcgttact actgcaggcc ttttgcgaat gctagccggt gggctttggt tcgataccag
     3901 cgctcttatt tacacaaaca ttttatacat cgttttattc cttttgccat tcacttttag
     3961 gtataatgaa tggtaccaaa gggtacttaa atatctgttt gtggttacaa acagtgtggc
     4021 cattggcgca aattgtgccg actttatcta ctttcgtttt accatgcgtc gtactaccgc
     4081 tactgttttt agtgagttta agcacgaaac aaattttggt tcccttatcc caaagtttat
     4141 tgccgattac tggtacgttt tcctgatatg gattgccctt actgctttac tggtattact
     4201 gtatggtagg gttcgtccta tcaataaggg aaaaggtttc aggtttcatt tagtttatgg
     4261 cttaaatggc cttgtgcttt tactggtttt tgctacactc atggttggtg gtatgcgtgg
     4321 tgggtttcgt catagtactc gtccaataac ccttagtaat gctggccagt atattaatga
     4381 gccacttgag gccgccattg tcctgaatac tccctttgcc atttatcgta ccctggggaa
     4441 aaagtcgctt gttaaggttg attactataa aaaccccaag gaactcgatg aggtttactc
     4501 tcccttacac aaaccacagg taaccgattc gctgaagcga atgaatgttg tcatttttat
     4561 acttgagtgt tttggtaggg aatacattgg ggcttacaat aaacacttaa aggaaaagga
     4621 tggctatacc ggatttactc ctttcctcga ttcgctcatt gggcactcgt tatggtttac
     4681 gcactcttat ggtaatggtc gaaaatcgat tgatgggatg ccctcggttt tggccagtat
     4741 acctatgttt gttgagccct actttttaac cagctactct accaacaaaa tcaatagcat
     4801 tgcatcagcc ttaagggccg agggttacca tacagctttc tttcacggtg cacccaatgg
     4861 ttcaatgggt tttcaggcat ttgccaatgt tgctggattt gaggaatact acggaatgag
     4921 tgagtatcct gacccctcgc acttcgatgg catgtggggc gtatgggatg aggagttttt
     4981 ccagttcttt gcgcaaaccc ttaataagtt tccacagcct ttctgcgcag cattattctc
     5041 ggtttcgtcg catcatccct ttaaggtgcc tgagcgttac gagggtgtgt tccccaaggg
     5101 aaccctaccc attcaccagt gcatagccta caccgattac tcgctcaaac gtttctttga
     5161 aaccgtttca cgcatggatt ggtaccccaa tacgcttttt gtcataactg ccgaccaccc
     5221 taaccaaacc cagttcccgg aatatcaaac aagcgtaggt gcatttgctg ttccccttat
     5281 attctacaga cccgataata gtcttgctgg tcaaaaggat gagtttgccc agcaaatcga
     5341 tatcatgccg tcagttcttg gatatttaca ctatagcaag ccttttatag cttttggacg
     5401 tgatgttttc gattccactt ccaaaccatt cgtggttaac tatattaata actgttacca
     5461 gtattactcc gatgattacg tgctgatttt tgacgggaaa aaaactttag gcctttataa
     5521 catcaaggat gatactatgc ttacaaacaa tctacagggt caacttcctg atagggtgaa
     5581 cgaaatggaa agtaagctta aagccatagt tcagcagtac aactcaagga tgattgatga
     5641 taatctagtg gtaaagtagg tgttagttgt aaaccctaac gtaatcgcca ctttttaaaa
     5701 cctggtatct ttttaatggg tacccttcct ttgggtaacc atagtttaca aggtagtaaa
     5761 tggttccgca gtgcggacag gttgcggtac ctgacccgtt tgaatcggga ttcaccgatg
     5821 cgttgatatc gatatgtttt gggcaagttg catcaaaagc taaaatttcg ttctcgctaa
     5881 gcctgaaaac aattacaccg ttgttcctgt aaccgtatcc atctatttta acagcaccca
     5941 ttacactagt cagattaaaa tactgtggct ctttcaggtt gaactcaatg tttacaggaa
     6001 catcaggaat aatatcatca atattgtcgt cgcaggcagt attagtaaaa accaaaattg
     6061 aaaaaagcaa aatatggatt atctttgttt gcattggtaa attaaatttg tatcttggac
     6121 aaaggtataa aaaagatact ttttgcgctt attgcacttt ttgttctggt tggttgtggt
     6181 aaacagttag caccctcgcc tgttcctgcc acccccggta ggagtaagtg gcaggttaaa
     6241 cgggaggaat accgacgcgc atgggttgca tattggcacg ataggaaaac cgaaaggtta
     6301 actcgcgggg ctaaagcaaa atcggttaaa agggagaggg aggctgaaaa ggcggagaaa
     6361 aagttggcgg agaggcacct caaaaaacaa ccacctgagg tgcaggaaag gatgaaggct
     6421 tcgctgaaag aggctgagaa aaacagacct aagcggactt taaggcagag gttaggcttt
     6481 tggaagtata aatagtaaaa taaatgatat ggatgcaggc gattttatat acattctcat
     6541 agcaatagca ttagctatac tcaatgcttt tgcgaatagc aagaagaaaa aggctgccga
     6601 gcaaaaacgg caggcacaga cttctgcacc tgccgatgat gacttgatta ccaaaaaatt
     6661 gcaggagtta ctgggtcagg aggttattgt tgaaaatgaa gagcaacccc tggaatcaac
     6721 tgttgttaat gaggaggaaa agtatatata tgaatacagc agcaaacctg aggttgaaac
     6781 attggatagg agcgttgagt tcgaaaagga gcctattgat aagccactaa ccgaggagga
     6841 actctaccct atacagcccc agaagccaat cgattcttca acccctattg agtatacccc
     6901 aattgatatt gccgaatcga aggtagaggg ccctattggc gattactcct accaggattc
     6961 ctttaatgaa agtatgcacg aggttgatat tgatgcttcc gatgctgaag aacttgagag
     7021 tgttaagaag gtggaagaag aaaaggaacc ccttttagcc gattttgatc cgctaaaggc
     7081 tgttgtttac gccgagatta ttcggccaaa atatttttag ttacaacata atggttggaa
     7141 ttttgaatta atttttttat atctttgcaa tcaaagagtt ccgatttccc tgtcgggatt
     7201 cttttttatt tcatatttag gaggtaaaat gagtaaagta acctatttaa ccgaggaagg
     7261 gctccagaag ttgcgcaagg agctagagca gcttaaaact gtggaacggc ctgcaatttc
     7321 aagaatgatt gccgaggctc gcgataaggg cgatttatcg gaaaacgccg agtacgatgc
     7381 tgccaaggaa gcacaaggta tgcttgagct gcgaatttca aagcttgaag atttgattgc
     7441 caatgcacgc attattgatg agtcaaagat tgatacaacc caggttcaga tactgaataa
     7501 ggtaaagctc aaaaacctta aaaccaacgc cgtagtggag tatacccttg tggctgagtc
     7561 ggaagctaac cttaaggaga ataagctttc aatctctacc cctattggca aagccctaat
     7621 tggcaaaaag gttggcgatg tggttgatgt tcaggtgcct tcaggtttga tgaagttcga
     7681 gattcttgga atatcactct aaatcatatt gctatggcta caatattcag ccggatagtt
     7741 agtggtgaaa taccctcgta caaggttgcc gaggatgaaa ggttttacgc cttcctcgat
     7801 attaacccct tggccaaggg ccataccttg gtaataccca aggttgaggt tgattacctc
     7861 ttcgatcttg atcctgaaac cctggctgga cttacactct ttgcccagcg ggtggccaaa
     7921 gctattgaaa gggttgtgcc ctgcaagcgg attggcgttg cagtactcgg tcttgaggtg
     7981 ccccatgcgc atatacattt agtcccgctg aataaggaat cggatatcga tttccataaa
     8041 cccaagctta agcttacccc tgaggaattt attgaacttg caaaaagtat tgctgcagag
     8101 ttcaagtaga tataataaca ataaaatgat aaaacgggcc aagagcccgt tttttattac
     8161 gattattgcc tagagtccct gaaagacttc ctgatatgca ttaaagaatg cccatgcatc
     8221 attcctggtt tcggagatat tctttccggt ttgctttatg tattttttcc ctacaaggtc
     8281 gtaaactatg gtatcggttt ggtttacgta gccaaagcct tcgttaaagg caaagtaggc
     8341 aaagggtgta accttacttg caagaatatt ctttgataga gtaaattgat catgaggcaa
     8401 tttaagctgt gccagaagtg ttgtggccaa atcggtttgt gagcatgtgg ttgtgacaac
     8461 caagggtttt ctttctacaa ccccgcccag ccaaaccata ggaataccaa actttaaggg
     8521 ctcccagttg ggatggtttg caggatgacg atgaccatgg tcggcaataa gtacaataag
     8581 ggtgtttttg aaccactccc tttgctgggc tttggctata aaatcgccaa gacagctatc
     8641 ggcatagtat gccgagctaa ggaattgttc cacctcggtc tttccattaa acttcggttt
     8701 ggcgggaatc tcaaagggct catggctgct aagggtgaag aacattttga agaaaggtgc
     8761 tttggctgag tcgatatcgg taagtaggta attaaaaaca tgctcatcgt gcactcccca
     8821 cttggagttg cggtagcttt taggaaaatc gtcctgtgtt atcagtcttt gaaatttagc
     8881 atggtaaagg aatgagcgaa tatttgcaaa atcggggtcg cctccgtagt aaaacgctgt
     8941 actgtagcct tggttgtaaa gcacttcgct aatggttggc agtttgttcg attttgtggg
     9001 gtacttgatt accgattggg tgggctgggc aggaaatccc gaaagcacgg caataatccc
     9061 tttgtcgctt ctatcgccgc tagcagtggt tcgggtgaaa agtataccct cggaagccaa
     9121 acggtttagg tttggggtaa cattgggaag cccaccaagg gggccaacca gctttgcacc
     9181 aaaactttca agtagaatga taataatatt tggttggttg gtgttcaaaa catataatgt
     9241 actatcggtg tgcatgagct caccaaagcg ttggtgggct gtggctttgt caatttcaac
     9301 attgtatgtt ttactcatgc tgctgctttt acttacggag tacatcatgt tccaaactgg
     9361 gttaagggca gcatggttgc aaaagttatt ctggctaaag tacactttac cggggtttat
     9421 gggtgcaata ccaaaacctc cccgaattgg aattatcatg gttgcagcca ggaaaatgta
     9481 aattggagag taccaccatc ttgccttatt ggcttggctt agttccctgg caacccactt
     9541 acggtatgca aggtgtgtaa ggtaagtgat tcctataagc aagacaacga atagtattaa
     9601 taatggcgtt tttaccgacg ccattgcctc ctttggggtt ttaaggtata gcaatggggt
     9661 tgcatcgata cggtaacccc aattgcggta tagttccata tcgctgataa caaccgtatt
     9721 tacaactaaa agtagtaata tggttaaagc attgtacatt ctagtaatac tctttgggga
     9781 aacccagaaa aagatagcca aaagcagaaa tgttagtaga agaatatatc ctgcaagcga
     9841 ggaatccatc caggctcctc gaatgaaaat atttatgaac tcgttaaact taaccgactt
     9901 aaggttttca aattggtaaa taaggaaagc tatacggtag gtggaaaaca gtattaccca
     9961 aaatacgaaa taccttaaac tgaatgataa atgttttctc atttgtgtaa atgatttaaa
    10021 ttgcaaacct aataaataag aacgtataaa cgtcacggtc atgcgcaaga aaattttagt
    10081 cttgttgttt attgttgggt tcgttgtaag caattcattc ccacaaacct ggagcatcgc
    10141 tgttcacggt ggtgctggga acatgaaacc cgaaaacttt accaaggagc agctcgatga
    10201 gtatgaacgg gagatgaaag ccgctcttgg tattggtgta aaaatcttga attcgggcgg
    10261 aacaagcctc gatgccgttg agcaggtggt tcggtacctt gaggattgcc cgttgttcaa
    10321 tgccgggcgg ggcgcagttt ttacccacga ggggcgtaat gagcttgacg cggcaattat
    10381 ggatggaaaa acccttatgg ctggttcagt tgctggcgtt accgatgtta aaaatcccat
    10441 atcgcttgcg cgcaaaatca tggaggattc cccccatgtg atgctggcag gtaagggggc
    10501 ttcggagttt gcaaaggtaa agggtattga aatcgtggat acatcatatt tttttactcc
    10561 tcaacgctgg aacgaccttc aacgtacgct atcgaaggaa aagatgggca ctgttggatg
    10621 tgtggcgctc gacatgcatg gcaacctggc tgctgcaaca tcaaccggag gaatgaccaa
    10681 taaacgatgg gggcgaatag gcgatgcgcc aattattggt gccggaactt acgccaacaa
    10741 ttccacctgt gccgtttcgg ctactgggca tggcgaatac tttataagat atactgtagc
    10801 tcacgatatt tcagcgctca tggaatacca aaacctttca ctcaatgccg cggttgagcg
    10861 tgttattatg ggtaaactcc ttaattctgg agggcgcgga ggagtaattg cggttgaccg
    10921 ctccggtaat gtttgtatga tgatgaatac cacaggcatg ttccgtgcat ttgctcgttc
    10981 cgatggaagt aatggatttg ccatttttta agatacattt tttgggtttt ttaattacaa
    11041 tttggtgtaa tttgaattaa atttgatgaa cctaacctta tcagtaatgt taaacctaat
    11101 ccggtaaact tatggcactc gaagttcatg taaaatcagc tgaaagcaag ggacatgtcg
    11161 agattttttt acttcacttt gtactcatgc tcatcgccat gtttgtattc cttgccttga
    11221 ttatgcttat aggtggttct tttacctggc caaatttctg gaagttctac atcacattta
    11281 ttcctttaac agtgatactc cctgttattg tggccataac cacatcggta tcgtataaaa
    11341 gggttaatgt tcacattttg cctgcctcaa aggttaacat tgaaaagctc aaagatttca
    11401 tgtttagcga ggattatatc attgttgata aggataagac tttcatacgg tttattcgaa
    11461 gaaaaaattt accccgatta ttaagtctaa acttcgacaa gccatcaatc acaatacaga
    11521 acgatgaggt aatagtaaat gtccttaaac gattatcttt agtattactc ccacagctta
    11581 ccattggtaa aaagtttata attgagcccg aaagcagtaa agaatgatta aaggttttcc
    11641 ttacctactc atcgatattc ctaagtgtga gcaaaacatt gccttcatgg ttcaaaaggc
    11701 cacgaaggca aaagttttat tacgtcctca ctttaaaacg catcaaagtt tagccgttgg
    11761 ggaactattc cgtaagcaag gggttaatcg gattacggtt tcgtcggttg ccatggctga
    11821 atactttgct gcaggtggtt ggaacgatat cactattgca tttccttttg taccacaaat
    11881 ggcgcagcgg gtaaatgcgc tggcgctggg tattgattta aatgtaactt tctcatcacc
    11941 tcaaaaccta ttagccacaa ttgatagggt tgagagtaaa gttggcgtat ttgttgaggt
    12001 tgatgttggt catagccgtt ccggagtagc tgttgacaat acccgtgaac ttgcccttat
    12061 ggtgaacctt atcagttcaa aaccgaattt ggtttttaag ggttttctta cccatgccgg
    12121 acactcctat agcgcgaggg gtagggagga agtagataaa cttcattccg aagctttaag
    12181 caagctggct aaactccgaa gcttttgggt cgaccagttt cctgacctta ccatatcgta
    12241 tggcgacact ccaacctgtt ccgtatccga tgagttttgg ggaattggcg agattcgtcc
    12301 cggtaacttt gtgttttacg atgtgatgca ataccaaatt ggctcatgca ccacttcgca
    12361 ggtggctgtg gctcttatct gtccggtagt tgacgtgtat cccggtaagc agaaaataat
    12421 tgttcatggt ggggctattc acctatcaaa ggacagggtt gggattgatg gagcggattg
    12481 ctatggcttt gtatgcagat ttgatggtaa aggatggagc aaccctattc caaatgctat
    12541 ggtaacttcc cttagccagg aacatggggt aatctcctat tccggtaatg acctcgatgg
    12601 tataaaacct ggcgatttac ttgccatttt gccagttcac agctgtttgg cggtcgattg
    12661 catggggcaa acgttcgatt ccgaaggcaa tacatacgat gttttaccta aaaataaaag
    12721 gtgatgaaaa ggttaactca gtttctgttg ttcagcgtaa tttctacatt aacaaccgct
    12781 cagtccacca agagcaatag tgtatttgat aagtatttcg agaataaaac catgagggtt
    12841 gattacattc acgggggcaa ttcttttagc gaggatttta aaatatttgg cataaagaac
    12901 gatggggagt ggggcggaag agttcgggtg atggatgacc cttacatcct ggggctttat
    12961 gcatttgata taattgatgc tgcatcggga aaggttttat acacacaggg tttttgcagc
    13021 gtttttggcg agtggcaaac caccgctgag gctggtaaac taaacaagaa ttttgaggag
    13081 agcgtcaggt tcccatggcc aaaggatatt tttaaattgg ttatgcgcaa gcgcgatagt
    13141 accaacactt ttcaagtaat ttggtctgaa actattaatc catccatgta tgccgatgca
    13201 aaccatgcct atcctgctgc taaggtgcaa tactttgtaa aaagcggtaa ccctaaggaa
    13261 aaggttgata tagttgtgct tgccgatgga tacactgtgg ctgattccgc cgaattcagg
    13321 aaagatgtgg aacgtttcac tgaggctttc ttttctgttg aaccatttaa gagtcgtaaa
    13381 actgatttta acgttgctgc ggtttactcc acatcaccca ctagtggcat tcgtctacaa
    13441 aaacacaact actacccggc caatgttttg gggtgcacct attacacctt tggaactgaa
    13501 cgttacgttt taaccgagcg ggagtggact gttcgggact ttgcttcggc agtgccctac
    13561 gattttttgg ttatcctatt aaactccaat aagtatggtg gcggaggtat ttacaacctt
    13621 tatatcaccg catcggcaaa atcgagcagt tccggatatg taatggttca cgaaatgggg
    13681 catcacatag ctggccttgc cgatgagtac tataccagcg atgtggccta ccaggtttca
    13741 gcccctaagt atgagccctg ggagtttaat attacagctc ttctcaatcc agcaagtttg
    13801 aagtggaaaa acatggttga acaaaacact ccaatcccaa cgccctggca aaaggaaaac
    13861 tatgagcgaa caggcaaatt gcgcattgcc gatgaagtgt actcaggtaa ggttggggct
    13921 tttgagggtg ccggttattt gagtaaaggt ctttaccgac ctgaggtcga ttgtataatg
    13981 ttctcccatt cattaacctt ctgtagggtt tgccaagagg gtcttaacag ggtgctcgat
    14041 atgtatgtgg ttaggtaatc ctgattccca ttactgtaac atcatcaacc tgaacattat
    14101 cgcctttcca ttcggaaaat atctcaagca aaaaatcttt ttgttttgcc atgggatagc
    14161 tggctatgga gcacaatatg tttctaaaat ccacaagtct aaacttttta ccattgggtc
    14221 cgccaaactg atcggtaaaa ccatcggtgt aaaggtagat aatatcatct ttttctagtt
    14281 gtatgctgtg cgtgacgaag cttaccttct cggttacgta accaattgat tgccgctcgc
    14341 attttacctg gtatagggtg tgtgtctcgt tttggttcaa aataatattt gagacttttt
    14401 gactattcga tttccgaaca atgttaaggt aaatattagc gccggagtac cttaattccc
    14461 ttgtttcagg gttgtagttg caaaaggcaa tatccattcc atcaaaaatt tcggagcctt
    14521 ccctttgctc aaacgattta tggaataatt cgttcaaatt ttgcagtacc tcgcctgggt
    14581 tggtctcatt aagttcgcta acaatttggt taagcccaaa catgccaata atactcatga
    14641 atgctccggg tgccccatgg cctgtacaat cggcaactgc aaaaagcatt tgattgttaa
    14701 aacgcttaaa ccagtaaaag tcaccactaa caagttcctt aggcaggaaa atcagaaagg
    14761 aatcgtcgaa aaaatctgtt ggctgttctg aggtttgtaa aatcgctttt tgaatcctgc
    14821 gagcatagtt aaggctatcg gttatgtcgc gggagcgttg ctcaagcatg tttttctgct
    14881 tttcaatctt ttccttttgc tgggcaagca ggaggtttgc tttttgcttg tttctgaatg
    14941 ccaaatacga aactactacc agtacaatta gtattgcaat aaaaccaacc agaaaaagct
    15001 gacgcaatcg cgatttgttc aagttggcct ggcttaataa aagattctct cgttggcgtt
    15061 gaatttcctt ctctttttga cgtaaggctg atatgttttc cagtctattg gcttcgctaa
    15121 taatttgatt gttaaatagg ctgtcagtta atgaaatgta tttcctgagg tactggtaac
    15181 ctaaggtttt gttcccctgt aactcttcca gttgtgagag ttccttgtat gcaagtcggc
    15241 taatgttagt tatactcaaa ctttccgatt gctttaggca ttgaagtaga tacttttttg
    15301 attcggaata gtttccaact ctggcgtgtg ccttacccaa aagcagtaaa gggtaaagtt
    15361 gcccgtaccg gtcgtttatt tcattttgag tatttaggat tgactgtaaa agcggaatcg
    15421 cactatttgg ttttagctga ttaattaata gttcagcacc aacagtttga gttctggcta
    15481 actccttggg gttgtttttt aggtaggttt ccgatttttt taaatgttta tcggccatca
    15541 gattgtttga ttgtctcaag ctattgttgg ccagcgctct gtatgttata cctaaatatg
    15601 tgctatccac taccactttc aggatggcta tggcattttc aaggtagctt tgggctgtaa
    15661 caaaatttcc aaactctgtg tgaataattc cttgcgatgt ataaattttt gctaggctta
    15721 atgaatcgtt gcgggattta aagaagttaa tagcagtaag gttgttctct atggcgttgt
    15781 tcaagcggcc aagttctcct tccacttttg agattgatag caagtaccag ccaattttta
    15841 acgaatccct tgtggcaatg ctgtagcggt aaccagggta tagataatca atcgattcgt
    15901 aaagcaaccc tttttcgata tggatttgcg aaaggaggta gtatgccttt tccaggtact
    15961 gttcgcgtcc catatccaat gagactatta acgcctggtt agcttcaatt agggcattgt
    16021 ctaaattctt cttattaaaa aggtattgcg attttgcaat taacgtctcc acaagtttta
    16081 ccttatcgcc attcctctca tactgacggg ttagattttc aaaatacttt aaggaatcaa
    16141 tattatctaa tggctctggt ttggcattca cccttagtgt tggtatgcca aatacaacta
    16201 aaagcagaaa tgctttgaag gtgataggtt taaacacttg ttaaaacatt aaagttatgg
    16261 tataaatgta aaaaatattt caacatcaaa gtcttgaaaa ttgactaaaa cagttcgcta
    16321 agcaggtcaa gcaattcatt caccttaatg ggtttagtca agtagccata agcacctcgg
    16381 gcaatagctt tgtcaatata ttcagctggg gtatatgccg attgaactaa aatgggaaca
    16441 ttgggtattt tttctttaat tctactgata agttctagtc cattggtgtc gggaagttga
    16501 atgtcgagta atacagcatc tatcttttcc cggttcaata aatcaatggc ctgagaaccc
    16561 gacattgcgg taaggctttt tgcgcctgag gcaattacta catcgctcag gtagttaacg
    16621 ttggcttcgt tatcttcaac aatcagtatg gttttgccct taattttggt atagtcgttt
    16681 ttggtaataa aatttatacg gctaatgttt ttggtattag ggattataag gggtagggta
    16741 aagctgaaac gcgagccctt attgggttct gattcaaccc aaatccttcc acccattagt
    16801 tctaccagtc gttttgatat tgccaatcct aaacctgcac cgccatagag acgttgagcc
    16861 gtttcgtcgg cctgtcggaa ccgctcaaat attttttcct gcagtgattt agggatgcct
    16921 ataccggtat cgctcacaaa aaactcaatg gtggcattgt ttggaatacg gtagcccatg
    16981 gtaacgctac cctgcgaggt aaacttaatg gcattgctaa gcaggttaag cagaacctgc
    17041 ttaagccgtt caccatcggc aagtatgtgt gttattccac tgttgttacc gatatcgcgt
    17101 gctattaaaa tattctgttt ttgtcttttt ttctcgttaa tggcaaattg gtgaagctcg
    17161 ttgaacaggc tatcaagctc cacgggttgc cttcgtatgg agtatattcc tgcttcaatc
    17221 ttggagagtt caataatgtc atcgataagg gttaggagta gccgcccatt actggtaatg
    17281 tagtcggcat aaatcttctt tttctcaggg cttatggttt cgcgggtaat tannnnnnnn
    17341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngtatcgct tttagtttta agcggcacag
    17461 ccatccagca caatgccggg gggccgtaaa aagtaagatt atgcttgctt ctaagcctgt
    17521 tcatctgttg ccggttcaga ataacacctt gaccgcgatg cgctacccac agggcaaagg
    17581 tgtttccgct tggataatcg gtgtttatgg ggtgggtgtc actaacatag gctaagctta
    17641 gcctttgtcg actcttatcg aacagcacaa ggtagatatt catggcatcg aaaagcgtgt
    17701 tcagctcttc ctgcattaac ctgtaaaaat cctcaggcga agtggtttcc tgtcctattg
    17761 cggatagttt taccagtatt cggtttatcc gttcaacctt attgcttaag gttatatttt
    17821 caatccagac taaaaaaatg ttccattcca caggtttaac atttgccctg taggtgtgct
    17881 gtttgccgtg tgtgctgtgt tcaaatgtgt atgtacgctc tttcttcgat gttttaaagg
    17941 gtaaattggg gaataggctt tcaattttac atccatccgg aataggttta tccgtaagta
    18001 aaagtactga tgggctatag tttaaaactg ttccatccct ttttaccagc agaacggcta
    18061 taggcaaatc atttatatct atgtgatttt tattgttttt tgtcattatt tttttccagc
    18121 gtttttggat gcaagcatca aaattaactg tctaatttag gaaatttcct ttaatccact
    18181 atacagtgtt aaatgtgttt aattttagat gggcttctac gtgttgaaat gttgtatcta
    18241 attgagctta gctgttgcaa tttatgtgta aatggtattt ttgcattaaa ttttgtttta
    18301 tatgcataaa atactatatg ttctgttatt tgcaattatt tccatttcat gtggcgaagg
    18361 tggcaaaccg agcagcaatt caatccatgg gaatggctat gcaaagcatg caaaaggttt
    18421 tgatatagag atagatggct cagtgactaa agtatgggtc aacaatccgt ttgaacctac
    18481 tgccaatggc ttggtttata cactgtatgc cgattcaatg cgaagcatag acccgaatag
    18541 tattcaaatt cctgtaaaaa gggttgtttg catgtcgtcc acccatgtag gttttatagg
    18601 cgctttaggt gccaattctg ctattgttgg aatttcggga acaaagtatg tcaataatcc
    18661 taaactctgg gaacgggttg ttaatggaca aattgccgag gtaggttacg accaaacctt
    18721 gaactacgag ttgttaaccc gcttgaagcc cgatgtaatt tttgtttacg gggttagttc
    18781 ggaatcgcta tcgtttatcg ataagctaaa ggagttgggg ttaaagatgg taatggtgtc
    18841 cgattaccta gaaacatcgc cattaggcag ggctgagtgg attaagtttt ttggagtttt
    18901 ttttggtaaa aaggagttgg ccgattcaat tttcagtgag gttgaggtaa actatgccaa
    18961 gctaacccag tcagtggaac aggttgatac taagccaaag gtttttctta atttgccatt
    19021 cagggatatc tggtacttcc cgggagttga taattacttt gtgaagctga ttaacgatgc
    19081 tggcggaagt tacattttcc cagagcttac aggctcccaa agccatccgg tgagcacgga
    19141 ggtagcgtac aaggctggtt taagctgcga tatttggtta aaccctggca ccgctaacag
    19201 ccttagcgat attaccaatt ccgataatcg tttttccatg tttaaatcgt ttggcaatgc
    19261 aagggtttac aacaacaata aaaggatggg gcagggaggc ggcaacgatt tttgggaatc
    19321 gggggtggtg catcctgagc aaatactaag cgatatgata aaaatatttc atcctgaatt
    19381 aatgcccacc gatacattgt acttttacag gcgattggag taaccatatc tttaaaaaaa
    19441 aaacaattac ttatgaaatt tggagtcata attgaaacta aggaacccga aaaggcatgg
    19501 aatgccttta ggtttgctgt ggcatcactg ggtaaggggc acgaggttaa ggtgttttta
    19561 atgggtgagg ctgttgagtg tcagggttta acccataaca aatttgatgt tgatgcacaa
    19621 atgcgcaagt tcacagctga gggcggaaca atcttggctt gcggaacctg ccttaagtcg
    19681 cgccagatgg atggaaccga gctatgtccc atatcaacca tggccgattg tattcaaatg
    19741 gtggaatggg ccgatagggt tatcacgttt tagccaatac acacaaagca atgcagggta
    19801 gaaccagaaa tacgatacta tttatctcgc tgatggtggt tttattacta ttagccctgc
    19861 ttagcttgct tttgggttca gtggcaatac cactgaccga tatcaaggcc tatttttttg
    19921 gtggcagctt taccgatgag gtgaatttat ccataattca aaatttccga atgccaaagg
    19981 tggtttccgc tttgcttgcc ggttccgctt tagcagtaag cgggttacaa atgcaaaccg
    20041 ttttccggaa tccgttggcc ggcccatatg ttttgggtat cagctccggt gctagtttag
    20101 gagttgcgct tgtgctaatg ggttcttcag catttgggct ttcgttttgg ggcagtcagg
    20161 taggaacggt tgtggcagcg cttgttggtt catttctggt tttaatgctt atactggcag
    20221 tgtccattcg ggtaagagat attatgacac tgttgatttt agggatgatg tttggcacgg
    20281 ctgtttcagc agtaacaagt cttttgcagt actttgggag cgaaacttcc ttaaagaatt
    20341 ttgtgatatg gacaatgggt agtttaggag gtgttggccg tcagcagctt tggctttatg
    20401 ctgcactggt tcttttagga cttgcacttg cctgggcgta cgttcgtccg ttaaatgtac
    20461 ttggaatagg cgacacctat gcgcttagcc ttggtttcaa tgtaaagcaa acgcggttcc
    20521 tgatattcct ttcaacaagc ttgttggccg gaacggctac cgctttcaat gggcctatag
    20581 cttttgtggg catagccgtg ccgcacttgg cgagaatggt ttttcgaact tcaaaccatg
    20641 ccattctggt accagcgtcg gcattggcag gtgctgctac tcttttactt tgcgatatta
    20701 ttgcacagct gcctgggtcg gccgctgtgc ttcccttaaa ctcggtggcc tcgctaatgg
    20761 gtatccctgt tgttatttat atcgttctaa aaaacaaacg cgtagttagc tgatgaacgg
    20821 tatagtgatt gacataaaag gattagtatc cggttaccga aatggagttt catcaacata
    20881 taggtatcca gcgtttaacg ccgtggcttt acgtggcgaa cttattgcgc ttattggacg
    20941 gaacggggtg ggcaagagca cgctgcttag aaccctagca cgcttacagc catcgcttga
    21001 tgggaatgtt tttgttaaag gggttaactt tcagcagata tcaggttcag agtttgcaca
    21061 gctggtgagt ttcatcccct cggagccagc gcatgcttcg catactacgg tttacgagtt
    21121 tgttagcatg gcccgttatc cctaccatgg ttggtttgag actttgaatg ataatgacta
    21181 taaattggtt gatgatgcaa tatcggccgt tggcatgcag aattttcggg atcggtttat
    21241 cgataacctg agcgatggag agcgccagcg cgtaatgatt gcttttgcaa ttgcacagga
    21301 tacgccaatt atcatgatgg atgagccaac agcttttctt gatttgccca ataagtttga
    21361 ggttatgcgt ttgctaaggg agcaagcaca aaagggtaaa actattatca tttccactca
    21421 cgacctgcaa accgcttttg gtttagttga taccatttgg cttatgctgg ccgatggtgt
    21481 tagggttggc gctccggagg atattgttct gtccggtaac ctcaaccgct taatggacga
    21541 tacgcctgta cttttcgatt tgaaatcggg acagtttgtc tataagcatc aaaccaggtt
    21601 agtggcttgt gttttgggcg gttcgcccaa tataaaaaag tggacctcgc acgcactaag
    21661 gcgtatggga tacgaggttg aattctcccc cattgacgat gctagtacct acgttcaaat
    21721 tcaggagcat gagggtaagg tgcaatggat acttggccgc gacgaccgta atcctacctt
    21781 cgattccatt cggaatctat gtatctacat taagggaacc acgcttctat agctttattt
    21841 caaagctatc ggcatcaagg tgaacgggga atgaacttct tacactatgt aggtaatcct
    21901 tatcgatgct tgaataaatt acttcctccg tgtttggttt agcctgggca ataatttgac
    21961 ccatggggtc aatgatttgc gagtcaccgg tgtggtttat ttggttgcca tcggttccaa
    22021 cacggttgca ggcagccacg taaacctgat tttcgatggc tcttgccacc agtagcgagt
    22081 tccaggcata cctacgacgc tcagggaaat ttgcaacaag tatcatcaaa tcgtaatcgt
    22141 tccagttacg gagccaaacc gggaaacgca ggtcgtagca tactgccagc agaattcgcc
    22201 agccctcatg ggttacaatc acgcgttgtg ttcctgatgt atagtgtgcg tcctcgtttg
    22261 ccattcggaa aaggtggcgt ttatcgtagg tttcaattgt gccgttaggg taggcaaaaa
    22321 ccatacggtt gaagtacttg ccatcagttt cggcaatgta gctgcccgca agggcaaacc
    22381 catgcttttt tgccatggat ttcatccatt caacggttcg tcccggatgc ctctcagcaa
    22441 gggaggctgg actcatgctg aagccggttg tgaacatctc gggaagtaaa accaggtgtg
    22501 ttccgggttc aatcccagcc agtttctgct caaactgctc aaagttatta tcgggcaatt
    22561 cccagtatag tttactttga attactgcta tcttcatttt ccgttctcca attaatgatg
    22621 cactgttcaa caagtgtctc gtaatgggcc gagatatctc ttaccaaatc aggtatttcg
    22681 tggtaaatgt gattgtcaat ctgcttaacc ggtaaaacac tgggagaggt gcccgtgata
    22741 aagcatccgg tggtttgctt aatttcatta gccccaagac tcctaaagtt aatgttgatt
    22801 tgcagggtgt tgcatatttc aatcaccttt tgacgcgtta ttccaccaag cactttgcta
    22861 ttgggggcag taattagttc attatcttta ataaaaaaga cgtttgatcg actcccctca
    22921 gtgataaagc catcgtggtc aaccagaaga acctcgtagc accggtgtgt ttctaaaatt
    22981 tcgttggcaa tggccctaat ggctttgttc tcaaacttaa cagttgggtt atgcctttcg
    23041 gcattgaggg tttcaaggcg aacccccgtg cttatctcat cggcggttgg gtagcgcgat
    23101 ggtataaagt atatcaatag ggtagctccg ttttcattgg aataatacat caacctaaaa
    23161 tttctctgat caaccgggtt tgctttaagt agttccctaa ttccaagggt gatctcatca
    23221 agatttatat tatagcccat catctgggca gagtacataa aacggtttag gtgatcgttc
    23281 agaaatattg gagtggagtt ttttacacga attacttcgt aaataatatt ttcaccctcg
    23341 gttagggcga cggaaaactt attgaggggt aatatttttc cgttataaaa gaaaaaactt
    23401 tgttgattat cgggaggaaa attcatgcca taaaggtttc cagcaaaaat agcacaagct
    23461 atttttttta aagcaatatc ctaatcaaat gtgggttttg gttttaaact tactgttccc
    23521 ttgatgtata aatgtctaat atattgttga ttaggggtaa ataaaaattg ttgaagaaag
    23581 cattccttgg tgagtttagg tgaagaatag agtttagcgt tttcccccct ttcagctttg
    23641 atgtggctat gctaacgctg ttttcggcaa aatcgcctgc aaagtagtag cagaacttat
    23701 cggaataccc tttcaccaca gctgggaact ggtttgttag gccaaacgat tttaattttt
    23761 cctcgcctgc tggggttgtt tccagcttaa aagtggcatg tacctctgaa ccgattccag
    23821 ccatgataat atcgaaccat ccgtaaaatt cagtttcttt tgaattgttt tgccattgag
    23881 gcatggtagg ttcggcaatg agtttaactc tggggtttag aaggtcagag ccttcctcaa
    23941 gcacatcaat taacccatcg ttgctaatca ggataatgcc tgagccttta cttggccaag
    24001 gtttctggtg ttgtttctcg tatagcttaa ccatccattc ggctggtccc ttaccgttgt
    24061 taattgcccc aagatttgga caatatttac cagcccaacc agtccagctt acattgaaaa
    24121 gttcttcggt tttggttcgg ataagtgcat ttgtggggga gctaaacatg ttgtactcgc
    24181 caataataag cttattgagt tccttcatcc gtttgaggag caagtagtcg ttctggttta
    24241 gacctccgta aacctttgat gtgtttgggg ctttggtttc ggttttatac cactcgtagg
    24301 agtaaactcc atagcagtcg gtatagtagg cggcatcgta aacggcagca taggcatcta
    24361 cctcgttcag tcgaatcgat tttatatcga atagttgctg atttaagtcg atggggaaaa
    24421 agccccagta atccgattta tggttgtacg atttgccatc ggggcccaca atccgaaagt
    24481 ggttgagcag ccagacaagc gatttgtgct caattctatc ggtttgggtt actgttttgt
    24541 caagtatata tatgttgagt ggctttgact tctgaaagta ccaggctagg tgaagcgcaa
    24601 taacaatacc aatgatacat gcaatagcaa ttccaaggta agtggcaatc tttttcattg
    24661 caattaggtt tctaaaactt gtttagcctt ggcaaattac aaaaaaatga aaaagattac
    24721 aagtgaaggg gataaattcg tgaaacgtga atcgttaacc gttaaccgtg aaccgttaaa
    24781 agttaaacgt gaaaagttaa aagttaaaag cttggcttta agtgggaatg agtcggaata
    24841 ctcggaatga gtcggaatac tcggaatgag tcggaatact cggaatatga tggagttaga
    24901 tgaagttaga tgaagttaga ggtagtttga gttcgtgaaa agttaaaagt taaaaggtta
    24961 acgcatggct ttaagtggga atggttcgga atgagtcgga atgctcggaa agagtcggaa
    25021 tgagtcggaa tacccggaat gtttcgtaat agacactctt ttacatacaa ccgtcaaccg
    25081 tcaaccgtca accatcaacc gtcaactgtc tacccaatta gtttcacagt gttacacgga
    25141 gtttaacaca gtgttacaaa gtgaaaatcc atcatatctg cggaatcagt gttcaatttc
    25201 caaacacaaa acacaaaaca caaagcacta aatactaaaa acatcaaccg tcaaccatca
    25261 accgtcaacc atcaactgtc aactatcaac catcaaccat caacttaatt attggcttga
    25321 gccttttggg attcagccag gagatgattt gatggcatat ttactaaaag atttttacca
    25381 ctaaaaccct tgcagtgttt gttgatttat tggtaagtga acgactttcg ttaatctcaa
    25441 caaacgtagc tgaatttggg gtaagtaaag ccgattcgtt atttacggtg agttcaagtt
    25501 ccccatcaag taggtagaat acaacgccaa atgggttggc gtgtgcaggt attacctcgc
    25561 ctggttttaa cgttaggtga actatttcca ctttagaact tgaaaacatt atacgcccgt
    25621 ccaaattgaa tggaaccttt ggcgcgcttt gtagggtttc gggtttaatc attttttcaa
    25681 ttatttaacc gccatataca tgtatacttt gacttgcaga gggcaagtat tttattccca
    25741 gccaggtaat tagcagaacc cccagtgcaa caatcattaa acgagccagt agcttgcgtt
    25801 tggtgggcaa gtaccggtga atatgaataa ctaaaagata gaagaaccag gtaattaatg
    25861 cccaaacctc ctttgggtcc catgcccagt agtcgcccca tgcaaactta gcccagtaag
    25921 cgcctaggag cattcccagc gttaaaagcg caaacccggg atatactagc cgcatttgct
    25981 gctggagttc atcgtgaact gaaccttttg aaaatgatgt ccaagccact gaaaggcttg
    26041 ctgatgcaag aactgcatag gctagcatat acgaaaccac atgtggtata aaccaggggc
    26101 tttgaagagc aggcataaga aacctaccct gatattctgg atgaatgata tcaacaacca
    26161 gaaaaaccga ggccatgatt aggcctatgg ttagaatgtt tttactgcga tatgctttaa
    26221 atacgcttag gctaacaagg cttaccaaga aagagtacca tattctagtc tcagccatgg
    26281 ttcgcatggg cggacgttta atatcgaacc agtataagat aataaatacc cccagaagag
    26341 cactggctgt aacaccagaa taccataaaa tgagtttacc ttttgcggat tctttgtggt
    26401 taaagtaaac agaaactaac caaagtaata tcacagtcag agcaacggga acaaaataat
    26461 tccaaaccat ttgtttactt ctttgaatcg ttaattaaaa ccaagcctaa tccacccagg
    26521 agcatcatgg caaatcctat gtatatcagt ggtaaccatg ggtcgtagat taccttaaag
    26581 gagctgtagg ttgagtctgg gccagccaga ttatcgtatg aatactggta aatccaccaa
    26641 ccatcaaccc taaaaggttt gtttacctca ataaggcatt ggtttacgga acttgatttt
    26701 tttgaataga ttttggcgta tgaaccaaag tattttgcct cggcaggtaa aagtcttagc
    26761 tctctgccat cgggaagtgg aagggtcttt tcggctgtaa catgggtaag gctcgagatc
    26821 cagcccattg taacagtttg cccattcgag tcaaatactt gtatcattgc cgctggcccg
    26881 gtaaagggaa caccccgcgc atcaatgaag gttgaatcgg ttgcaaatgc cttgggtaag
    26941 tattgcataa cctgaatggt taggttatca attttaatgc ttgggttact gtttaggtcg
    27001 tattccgatt ctgggaagaa tgtcttacta cccattaaag ccaatcgagg ttggtagaat
    27061 tgtgcttcaa acttttgtag ttcaattgct attggcaatt caattacact accgttcttg
    27121 tctgttccgt accatgcaat ttcattctgg ttaacctgaa cggttgcctc aatcttatcg
    27181 gaacttccta ctaaacctgc cgataagcaa acccaaagcc cggcatggtt aagggtgaaa
    27241 attatgtttt ttagggtaat tggaaataca cggtttacaa cagccgcccc taaggttatt
    27301 agcataaaaa ttaaagggaa taaaaacaac caggaaccca gtaggttaaa taatgccccg
    27361 ggtattccca taccatgtcc tatctgtggg ataattccca ttagaataat tgagaagaga
    27421 aataatgaaa ttgatggaat agctgactta accgaggcaa gccagttgaa aaatttcgat
    27481 tttgggaaaa tgatttttaa gaggattaaa aggctaagaa ttgtaactaa aacgatagca
    27541 ttggttggga aacctagttt aggaaatagc gatagaggga caaatagttc caaaaagaaa
    27601 gctagtaccc ataacccaac ggacacaata aaaccctcta aaaaacccca aggccatagc
    27661 caaaggggtt tctttttaaa ttgattgccc atgattttga ttgctacatt attctttcag
    27721 gcaaaggcat gttctgttca cgctcttttg ctaattgtaa ccacttggga agcacatttt
    27781 tcttccattc ttctttctcg cccttaagtt ttttcagatc aattcccaca taggcttgag
    27841 ctttttcctt tgatgaaagg tctggcatgg gaacaggctc attgtgtcct aacgttgcaa
    27901 gtaccctgga gagctgtaag cgcgcttctg aggctttctc tattccatcg gcaattattc
    27961 gcatactctc aagaggagca tggaacgatg ctccatggct tgcggctaca aaatcccaac
    28021 gccactgtgc ttgtctgata agtttcaata ctggctccat cattttttca ttggcgccat
    28081 tgtcccatgc gaattttgct tctatatgtg cctttaccaa taagtcttcc agtacaattt
    28141 tttgggtata tactctgtcc tgcctatcgt aaacgttctt aatgagttct tccttggttt
    28201 gtttatgaca aaccatacat gaggcctcca tgttgttcag ggggctttga accttatggt
    28261 cggtaatttt ctggctacct tctgtaacgt atggcatatg acaatcggca catgaaaccc
    28321 cgcgctggta gtgaatggaa tgttggaaaa gctcaaaatc ggggtgctga gcctttatga
    28381 ttggagtttt gcttaagggg tgaatccagt cggcaaatgc gtattcgtcg tagtacttct
    28441 caatgtcatc aacgttaagc cctttatccc aagggaaggt tacatattta ccatcgccct
    28501 taaagtagta ctccacatgg cactgggcgc acaccatggt gcgcatttta tttagcggca
    28561 acaactcaac gttactaccc atacgctcat aagcttcttt taatgggatt tggaacaggc
    28621 gtagagtcat atcgcttgga ttgtggcagt ttgcacaacc aatagggttt acaatttccg
    28681 agagcatagc cgaccacttt gttttgtaga aatcggctgg cgacatttta gccataaccc
    28741 ttgcaacatc ggggctcttg caagcccagc aacttgaatt ttgagggccc tcattctcat
    28801 tggcaggtgt gccagtgcgt aacgtattat gaacatccgc cactgagtat acatgacctc
    28861 gggcctgatt aaaatctttt gaaaaggcat agccggcaaa gagtattacc agtcttgggt
    28921 cttcgtcaag taaatcgcga aaagcgcttc cgccatattt tgaccggaaa agggtatcgg
    28981 acatctggga gtaggtttga tactctcgcg gaaatgccat accccatgcc tcgtttcggg
    29041 gctcaaactc atcgatctta taccttggag tattcattag gtttgcttct gttcgtcgct
    29101 gaacaatgct ggctgccagt aaaccaagaa caaaaacaac aatcattgtt cccagaaata
    29161 atgcccatcc aatccatgga ttcttggcaa ttttttcact taacggagtt ttcatgttgg
    29221 tataggttta atatctattt aatcagtttc tttaaccagt taggggtgtc agttttaggc
    29281 actggtgcag tgccatatgg tgttgcggaa aggcttttta ctgtgccatg gggtacatca
    29341 cggtgacaat cccagcaacg tttcccattt ccggcaagaa ttgcatctaa atcgacattg
    29401 gctttaacca tggctgtgag gtttccatgg cagcgtatgc agttttcctg aaccactctt
    29461 tgcccaggct tatgcatgta aatggtttgg gggtaagtgt ttagtgtaaa gatggtggca
    29521 tgccgcaaac catcgtttgc tttaaagtaa tacttacgga cgatgttgtc gtggggtacg
    29581 tgacaatcgt tgcaggtggc gtgttcccta tgcgatgagt ggtgccaggt aacatattgc
    29641 gtcctcatga tatggcaatt gacgcacgtt gcagggtcgt cggacacata cgaccatgcc
    29701 ctggaggtat aaaaaacaaa caatcctgtt ccagtaaaaa ttcccgccaa tataattact
    29761 ggtactcgcc agtttggtgg gggttgaaaa cgttttagta tctgtttagc ccagtcaaac
    29821 atattgatgt tttttcaaat ttaggtaata agaagcttag aatcaattta aatatttgcg
    29881 cttataccac atcataaagc ccgatagtac tactaccaca aggaaaatgc ctgttaatgg
    29941 aaccaccagt atgtaaaagt cgccaagcat aaagctgaaa attcgaccgg tatggatttc
    30001 ctgagccaga ttccacagcg ggaatttgaa agcatcagca actgttgatg gcattggaat
    30061 agcaggctct gaatgatgta aagcaaccat ccctttatcg taatcgaata tgtattctaa
    30121 ccctgaaata tgagcgtatc ccgatactac attctcgcct aatggttttc gtaggccgtt
    30181 ttctcttcta tgcaggttgc ctgttatgta gttgaaggtt tctcccgtgt cggggttcca
    30241 gcggaataat ccgctaaagc ttcccaccag gtaaaacacg ctgtctagag gctcaagaac
    30301 attgataccc attacgctaa ctggtggctg attgggaaat ttggttaagg gctctgagaa
    30361 gtcagtgcaa tggtaaattc catcggacgt tgccaggatg aacttttttc tagctgagtc
    30421 aaatcttata tcgcgaagtt tatcgttcca gtagtagctg ttagaaacct tttggttgtt
    30481 aagcactatt tgactattgg caatggttag caggaaagga ggacgcagga aagttcctgt
    30541 taggcccgat attaccaata gcaatgtagc cagtgctcct acctttaagt gccatttata
    30601 agagaaacgg gtggtgcgtt ttagtttatg gaacctgaat tgctcatggt accttttgat
    30661 tattttgggg gataaaaagt agataatacc tgttatgctt aagaaaatca tgactaaagc
    30721 aaccaaatcg gctaataatc gtccgggaat tccaaaaatc tcgccgctat gaatattcca
    30781 aaaaagccgg aaggttgata tttccttttt ggaacctaga ggggctttca aatcgattgg
    30841 cgtaaactgc agggtttgtg ccgatgaata ataaattgaa ttacgtgttg taatgtatat
    30901 acttgattcg accacctcaa ttgaggtgca gaattcatcg ctttggggta ggtgcaactt
    30961 tacccaatga ttttcccctt tgccaagcca gtacaaaccg aaacgggtgg cagcaagtat
    31021 cccgtttggg gttacttcaa ggtcaaaaat ctttctgtaa tcgctccctt ccgggatacc
    31081 atccattaag ggcttccaat tttcaaaatt gcttttggta agccaaatcc caactccccc
    31141 ataaaggatg atagaatcag agttaatggt tcttgctcct tttaccgatg ccaggttcca
    31201 gttttttaac tggtagttct taggtaaata cttacggttg atatcgatat gggcaatggt
    31261 ttgccggtga ttcatcaaaa ctcccgaaat ggcaaaaagc aaaaaaaaca atgtaaatac
    31321 taaagcgggc catttgtggt acttccgcag agtactataa aaactcataa tagcaaaagg
    31381 ctttttcttt cagaaaattg tttacggttt atagaaatag atgttaaagt gttcatcgtc
    31441 aatcttttct acccagtgtt ttattcccaa gctcgaagcc ttatctatta agggtgcagt
    31501 aataaatggg gcaaccactt tgtatatttt atcagaactc aggtcggtta aatcggccat
    31561 tatctggtta actggttgct caccagcctg taacatcacc cttacatcaa tttctgagtc
    31621 aactttgttt gaatcgaacc attggggttt ctgggtggtg aactccatct catcggcacc
    31681 ctcaagcaag tcctgtccta cagctttccg gaggacatta atgagttcct ctaccttaac
    31741 atttcctatt gctgcagctt gctgaagtgt tgcaacacga gcaactgttt tccttagaac
    31801 aggatttttc agcttttcaa aagctggaac atacccaata agcacctcct cgagctgcgg
    31861 gtatgcttca attagctgca aaaccttggt tttaggtgtt ataatgagtt tgttttcgtc
    31921 catagctatt tggaatatga tagaatacgt tgttcacctt caagtttgcg atacactgaa
    31981 acattgtgcg aaacctccaa aacaccaagg tattcgcctt tctcgtttcg taatgcaaag
    32041 tactcgatat gtattaggtt gccaccaata ttaatccaga agggggctct agaagccttt
    32101 ccgcttttaa agtcctcaat gattttatca acaatgtgag cactggcagg gggatgacat
    32161 aatcttacat cgcggttaag gatagccctg ttacgctgga atattctttc ggggctttgc
    32221 gagaaatact taaccttatc gtttttatca acaaatgtga tatcaactgg taatgtgtta
    32281 aggattgcca gaagttcttc cactgagaaa ctacccgttg ggagttgaat gttttcggcc
    32341 gttttgttta actcctgaag ctcgctgcct tcaacccatg atggtttcca ttttgtagga
    32401 gggtcgtata ggcaaaagcc tatctcaagc tgctgcttat gaatctcgta ccagtcagct
    32461 tcagtaagct tatccaaggc catggggaaa agaatttctt cctccttggt agtcatttcc
    32521 ataacgcctt taatggcagg gcggagcaca atctcgcttg aggcaattag ttcgtcgcgt
    32581 gatatccctt cggtttgcag gagttcaatt gagcctttaa taagctctct tatttcatcg
    32641 tgttttcccc acattacctt tggagggcca gtaattccct gcttttcaag aaaggggaaa
    32701 agtaggtatt cctttcgctg atagtgctta tctacatcaa acagagcgtt aaactgtccc
    32761 ctaagcttga gtacaagctt tgggatgtca actgcttcac ttgacacaag ttccataagc
    32821 gattgctcaa tggattggca aagctggttg agttctttat tttccttaat gaacacatca
    32881 acagggtgac cgtcgggaat ttttttaacc gttgtaagat caatatttcc cttcagcact
    32941 gctgagtgta catcgcaaag tttcaggact tcctgttccg gcaaaccttc gctaatcaat
    33001 tcctgctcaa cctcaactac ctcgccatag gggatattgg tcaggctttg tattagctcc
    33061 tgccgcactt cctgttctga gtcgccttta tgtaaccgga gaatgagctc tttgagcttt
    33121 ttcttcctgt tttcggagtt attaatcagt tcgctcatgt tttttctttt aaagttagga
    33181 ataaaataag cgcaaggcaa gggaattgat taaaatcaac tattccttta tcatggttaa
    33241 aaccatttta aacctttcct cagcctgaag cgcatggggt atgtttgcag gcatgataat
    33301 tgtttcgcct gcaacaagtt ggtggggtgt tccaccgatt attattgtgg ctttaccttc
    33361 aataacctga acaacggcat caaaaggtgc tgtgtgctcg cttagaccct cacccttatc
    33421 gaatgcaaaa agcgagatgt tccccgttgg gcgttttagc acgtttttgc ttactatgcc
    33481 gccattgctg tattcaacct cattcataaa gttgaacttt tgtccttttg gaaattcatt
    33541 tgttaagttc atagctgttt ggatttatgt tagatcaatt taatagaaga aacccagccg
    33601 tagtgcatac ggctgggaga gagggctagc ctaccattag cttgatgtct tcatctacag
    33661 tagtgattcc accaattcca aagttctcta ccaatacttt tgccacattg ggagagagga
    33721 atgcaggaag tgttgggcct aggtgaatgt tctttactcc caggtagagt agcgcaagaa
    33781 gaacaattac atttttttgt tcataccatg caatgttgta ggcaatgggg agttcattga
    33841 tatcgttaag ctggaatacc tcttttagct taagagcgat aactgccaaa gagtaggaat
    33901 cgttgcattg gccggcatca agtacacgag gaattccacc tatatcgccc aaaggtaatt
    33961 tgttatagcg atattttgcg caacctgcag tgaggataac tgtatccttt ggtagtttct
    34021 cagcaaactc ggtgtagtag ttgcgatcct tcatgcggcc atcgcatccg gccattacga
    34081 taaacttacg aatagctccc gattttacag catctattac cttgtcggcc aaggcaaaca
    34141 cctgttcgtg agcaaatccg cctataattt cgcccttctc tatttcaaca ggaggtttgc
    34201 aacgctttgc gtgttcaatg attgcgctga aatcctttgg tttgccattc tccctgtcgg
    34261 gtatgtgttt tgcgccctca agaccggctg caccggttgt gtaaatccta tccttatagt
    34321 tggcgttggc aagtggtgga acaatacagt tagtggtgaa caggattggg ccattgaacg
    34381 actcaaaatc ctcgcgctgg tgccaccagc tgcttccgta gttacccaca aagtgtttgt
    34441 atttcttaaa agcagggtag tagttggcgg gtagcatctc gctatgggtg taaacatcaa
    34501 ccccagtgcc ctcggtttgg gcaaggagtt cctccatgtc cttaagatcg tgaccactga
    34561 ttaggattcc cggattgtta cgaacgccaa ggtttacctt ggtgatttca gggttaccga
    34621 agctggtggt gttggcctta tcgagcagcg ccatggtttg cacgccatac ttcccggttt
    34681 caagggtaag ggtaacaagt tcatcaacgc ttaggttttt tgtaatatcg gccatggcct
    34741 tttgcatgaa tgcgtaaaca gtggtgtcct caaagccaag gttgtaggca tgctcggcat
    34801 aggctgccat accctttacg ccgtaggtaa tcaactcctt aagtgaacgg atatcctcgt
    34861 tggtttcgtt cagaactccc acggtggcag ctttagcatc aaactcttcg ggcttacctt
    34921 cccatgtgca tccctcaaag gtggtgtcaa cctttacccc tttcttttgg gcttcctcgc
    34981 gcagcatgtt acgaatctct atggcctttt caacacgctt cgtgatggct gccttatcaa
    35041 aattggcatt ggtaatggtg gagaataagc tgtcaaaaac aaacttgtta accatagggc
    35101 tttcgtaacc cttttgctcg cggagcgctg tgctgtaaac gcaaatcccc ttggttacaa
    35161 agagcaggag atccatcatg ttagaaagat cgtcgttttt accgcaaact cccttgattg
    35221 tgcagcctac tcctttggct gcttcctggc actgatagca aaacatactc atagcttgat
    35281 tgtttttagt aaatagttaa atgtagtaag tgaattattg attttgttga ttatttgctg
    35341 attaaaccca gtttgactct aatatttcac cttgaattcc aacggtaatc accttaatag
    35401 gaaccttgcg ttttgcctgt tccatggcca tacgggctag ctgaacaagc ccaccgcagc
    35461 agggaacctc cattttcatc acggtgatag tgtcaacctg tgcatcgtca attagcgcag
    35521 taatcttctc aacatacgat tccttgtttg agtcgagctt agggcaggca atagccaagg
    35581 ttttaccctt caggtgttgg ctatggaaat tgccaaggct gaacgccaca cagtcggctg
    35641 caagcaacag atttgagcca cggaaatgac ccgagtgtgg gttaatcagg tgtagctgta
    35701 cgggccagtg ggttagctcc gatttccccg aaaccattcc ctcagcacct gcaatggctt
    35761 gtgcaaagct gcgagttgcc gaacccggac aacccccacc atggtgatgc atgggttgag
    35821 gctttacctt gggctcgctt gtcccacaac cgcaggcatc cttggtgtgg ttatgcactg
    35881 cttgcagcac ctcatctacg ctaaacgtga gtttgtcgcg gttggctaac aaccactcaa
    35941 caccctggcg caagtattcc ttttcgttat aatccttgag gtgctttagg tgagcaatga
    36001 tggtattctt accgtttgcc accatcttac taatggtgtg tacctcgtcg taaggctcag
    36061 cctcgcggtg ctcgatggta atggcaccct cggggcattc gcctatgcag gctccaagtc
    36121 catcgcacat cagctcgctg atcagaacag ctttgccatc aataagctgt agggctccct
    36181 cgtggcaacc actaacacat agtccgcaac cgttgcactt gtccctgtca attgtaataa
    36241 tgtcgcgctt catatctatt cgtttttaat tgttatcaaa ttgggttgat ttgtttttct
    36301 gagacaaaag taggccaacc ccactccgcg aattgtaaca tatgttacac acgctgcgga
    36361 ttttggagtt tttagggttg atatatatca atgccggaag ctgtgcggcg tatcaggctt
    36421 ctggaaacct agtggctatt agtgtttgaa gatagtttat agttgacggt tgttagttga
    36481 tagtggagat agcggtttga ggactccctg taagtgagaa tcttcgttgt aggcgatggg
    36541 gtgttgtaaa aaaacttttg gtattgggat gtttccccgt agggggaaaa caaatccctc
    36601 aaatactctc aaataaaatt aaggaatctt agttccggtc aaaaattccc gaatatttat
    36661 ggtttcaatt cctttgtatg tgtcgagggc gtattcatcg gccgtaacta caatttttct
    36721 gaaattatcg cccgtttcaa gcagattgcc atattcgcgg gttctcacat tttcatcggc
    36781 caacgagagc gttacctgga tgtagattcg gttgctgttt ttatcgcaaa tgaaatcaat
    36841 ctccttatcg cctaacttac caatggtaac actgtagccg tgtataagca tgtgcagcag
    36901 aacaatgttt tccagcaatt ggttgatatc gcctggttta tacgatatta tggcgtgtcg
    36961 cagacctgtg tcgtggaagt agtatttctc gccaatctcc agtattcgtt tcccttttaa
    37021 atcgttgcgg gagattttgg caatcagata cgcattttgc aggtagttca gatagtcgag
    37081 aacaatattg ttcgacgatt taatattttg cgatttcagg taatcggtta tctttttagc
    37141 cgaaaaaaca ttaccaatat tgtcggcaat aaaaatgacc aaccgctcca gaaaggccgc
    37201 gtttcggatg ttgtacctgg taattatgtc cttgtagagt atggtagtat aaatatttcg
    37261 cagatattca aaaaccacat tctcgttcaa cgttaggttc acaaggtagg gaagtccgcc
    37321 aaatcgtagg tatttgttga aattttcagg agtgttttcc aaccggttaa acacgagaaa
    37381 ttccggatag gaaaggctgt acatggtaat ttcaacatat cgcccggtca gataggtgga
    37441 taactcaccc gaaagcagct tcgcgttact gccggtacag taaatgtccc acattccggt
    37501 ggtgtggaaa tgccgcagtg ccttttcaaa gtcttcaata tcctgaattt cgtcaataaa
    37561 aatggcggct tttttcccct ttgttacatt actttcagca aacgaaacaa ggtcagcata
    37621 ggtttttatg gtgtcaaatg ccaaatcttc cttgtttatg taaaggattt ggatatccgg
    37681 atttagctgt tgtaccagat ccataacctg aaacagaaag tagcttttcc ccgtacgtcg
    37741 ctgtcctaca atcactttaa tcaaatcctt gccgataaaa ggacgtatct ttgcttcgta
    37801 catttctcgc cggatatact ttttcataat aaaagttttg attatgataa aaataatttc
    37861 aaatctacgg aaatatttta gtatgaacaa aatatctctg aatttgtttt ttttatgttc
    37921 caaatggaag gttcttcaca tccttttgat ttctggtata atatgaggag ttgatagttg
    37981 atggttgata gttgatggtt gatagttgat ggttgatagt tgatagttga tagttgacga
    38041 ttgatagttg acgattgata gttgatagct gttagtttat agtggagata gcggtttgag
    38101 caccctcagt aagggaacat ctttgtcgaa ggcgattgag gaatgtaaaa gatcatgata
    38161 aaaaaacagt atcccccgta ggggattaac gatgaatcaa taataagaat atcaatacat
    38221 tactacagtg ttacatggtg tggcacaggg tctcatagtg taagattata aatctctttg
    38281 catttactat caaccatcaa ccgtcaacta tcaactcaat tagcatcaca gagttacaca
    38341 gggtttaaca cagagtttaa cacagtgtta cacagtgaaa atccatttaa tctgcgtcat
    38401 ctgtgtccca ttaccaaaca ccaaacacca aacaccaagc actaaacact aaacactaaa
    38461 caccaaacac caatcactaa tcaaccatca actaacctta tccctttact tcccttgcta
    38521 gtacttaccc cttaaagaac agctatttca gctcttcttc caagattttt tcgagcgttt
    38581 ccacgcttat tcgcttggcc aaaatctttt tgtttttgtc caaaacgtaa attacaggcg
    38641 tggcatagat atcgtacagg ttgcggaagt ttgagctgaa cgtaggatcc caaacattta
    38701 tccaggttaa ttcgttggcc tttatgtatt ccatccattt gggctggtcg ccttgggtgt
    38761 aaacggcaaa cacctcaaac cctttaccct tatatttctc gtaaaccttt ttcagctcag
    38821 gggtaacctt tttacagtgg ctacaatcgg gatcccagaa gtatgcaaca gtaaccttgg
    38881 cattcacatc gcttaagcgg tagtatttcc cattggggtt tggtaaccaa aggtcagggg
    38941 ctatattgcc aataatattg ggctttatgg cttttacacg ttcggcaatc ttatcgagca
    39001 gtttctggtc ggcccaccag gcttggcctc ccaggtagta cttttcagcc actgccacaa
    39061 atacaccatc cattcccatt cgttccgagg tttgaaactt attggttagg gtagatagaa
    39121 tatactggaa catctcctta ttggcctttg ccttctcaat tagttcaatt gctgctggga
    39181 taatgctatc gggcatgggg agtagaaccc tatcgaaata cacatttatt ttactctcca
    39241 gtatgggggt tcgtagtagg cgagcatcgg aaaaatcgat attatcaaag aaatggtacc
    39301 ggttatagtt gtagttatgc gcccaaacca gcgaatcgta ctttggagta tccttggcaa
    39361 tattgtgctt gggtgcttca acattccgca tggcttttat aattgctgca agcagggtgc
    39421 ctttgtagtt ggcttccacc tcgtcccagt aagctaaaac cttgttgtta agctcgttca
    39481 ttttatcgcg gatggcattg gccttaagcg aatcggaaag cgattccttc agctgttccg
    39541 agagttcctt gctctgcttc tgcatgtcaa tcatgaaacg ctggtactta atgaaccctt
    39601 cgttttcact agagttggtg aacttgaggt tttcgagtaa gttatccttc gatgtggcaa
    39661 ctgaaaattt ctggttctta tcaacaagca cctcaaagta gtttttctct ggcagtataa
    39721 caaggtatat accaccaggc aaaagggtgt cgccttcaaa cacggcaaat cctttgctat
    39781 ttacagtggc ggtatctttc acatacttct tatcgccaaa gtggtagcct agcaataaat
    39841 cgccctgggt taatccatca attttaactt caatcctgta tccgtttttt tgtgcaaatg
    39901 cacttgcaaa aaccattaag ctcaatgtga gtgtaatcag gaaaaacttt ttcatagttc
    39961 attcaatttt catggaagca acaagcaaag atagcatttt tgaaatgagc caatattgat
    40021 agccctatat ttcaacaaaa atacaacctg ttttgttagt gtatcaaatt agataccaaa
    40081 tttgatggat acctggaaag atgcccggcc aaaccgatta gttcgtgagg ttagtccata
    40141 ccttttacag catgcatata accctgtgga ctggtttccg tggggcgagg aggcttttgc
    40201 gaaagctaaa gcagaaaata aacttttgct cataagcatt ggttacagca gctgtcattg
    40261 gtgccatgta atggagcacg aatcgttctc ggacgaaaag gtggctaaaa ccatgaacga
    40321 gcgatacgtt tgcgttaagg ttgaccgtga ggaacgcccc gatgtcgatc agatttacat
    40381 gaatgccgtg cagattatta cccgtcgtgg tggttggccc ttaaactgtt ttgccttacc
    40441 cgatggccga ccggtttacg gaggtaccta tttcccaaag gagaactggc ttagcattct
    40501 tgagagtctc gatgaaacat ggaaaaacga gcctcagcgc atttacgagg ttgccgatga
    40561 gctggccgag gggattgcaa acactgagat tatcagggtt aaggctgatg tcagtaatgt
    40621 taaccttaat gtcgttttag ctgaaaacct caattccttc cgcaatcagc tcgactttaa
    40681 gttgggtggc acgctgggtg cacccaaatt tcccatgcct ggtttgctga aatacctgct
    40741 gataaccggg caacactccc agcgaaagga cttacttgct tttgtcgata ccacccttga
    40801 gcgaatggcc aacggcggca tatacgacca cgttggcggt ggatttttcc gatactcggt
    40861 tgatgaaaag tggtttgttc cccactttga aaagatgctc tacgataatg cccagctaat
    40921 tgaggtttac tccctggcct ataggctcaa cccaaatcct ttgtatagga gagtggttga
    40981 ggagactatt acctttgttg agcgtcaact tctatcgtca actggtggtt ttttcagcgc
    41041 actcgatgcc gatacggctg gtcttgaggg tggttattac acctggggga aaagcgaggt
    41101 tgagcagata ttgggatatg actcagagct tttctgtgtg gcttatggta ttactccaac
    41161 cggtaactgg gaaagcacca atattttatt ccgctccctt acaaatgccc agctctcgag
    41221 cctttttgga ttacccgagg gtgagattga ttacaggcta aaaacaagcc ttataaaact
    41281 caagaaccat agggatttcc gtgtccctcc ccttgtcgat gataaaatta tcacctcgtg
    41341 gaatagcttg atggtttcgg ctctttccac tgcatatctt acctttggtt acgataaata
    41401 ccttcaactt gccttgtcat ccgcaaatta tctgatagat aaccatgtta atgatgactg
    41461 gacacttagc cggattacat gcaaaggtag ggtttatggt actgcattgc tcgacgatta
    41521 cgcctatact atcaatgcgt tgataaatct ttaccaaatc accaataata ctttatggaa
    41581 ccgttatgct attaacctgg ctcagaaaac caaggatatt tttttcgaca ccaattcagg
    41641 aatgttttat tacactccag ccagtacaga acttattacc cgtaaaatgg agctgatgga
    41701 cggtgttatg ccttcggcat cggctgtaat ggtaagtaac ctgcttgcac ttgccgagca
    41761 cgatactagc agtgagtatg gcacaattgc attgcaaatg attgctaacg tgggaaacca
    41821 tattaaacag ggcaatgttt ttgttcatac atgggcattg cagcttttga gtaccatttt
    41881 gccagttgct gaaatcaagg taaatggcga tgatttgaat gctgccgaaa tgttgatagc
    41941 ctcaaaattg gtttacccta agataaagtt cctgcaatca accgatatac ctaacaagta
    42001 cctgctctgc ataggtaata cctgccagtc gcctaccgat aattccgaaa atattgtaaa
    42061 gtggactaac gctattagct ttgagtacta tgtttagttg gaacgcataa agcgccttgg
    42121 tcgctttata ttaaaatcaa atctcttgtt tcaattgtac tgttatccgg ataaagtcac
    42181 actaccgccc gcctaatgaa tttatgaatt agcaaaatct ctatttaatt tatcctcttg
    42241 tagaaataga aaatgctagg gttgatttct caaaccacac tttatcgcct ttgaccaagc
    42301 gaaatgatag aatacgccga aatatctaaa aatgatagat taatagagat ccttcgcttc
    42361 ccgctagggc gggacaggct gctcaggatg acaagcggag ttgacagttg atggttgatg
    42421 gttgatggtt gatagttgat ggcaaaaaca ctaaacacta aacactaaac accaaacaca
    42481 aaaaactaaa cacctctgca cactgcacac taattttctc tctacctttc taaactccct
    42541 ttttattttc aaaagattta tcctaatttt attgaaaatt tcattgaatg gcgtactcgt
    42601 ttgattcata tagggttgaa gttcttcaga ggccttttgt tcgttccgat ggctttacca
    42661 ctaagcaaac cgcaaaaatt gaactgttca atagtaaggg tgttttaata acaacattaa
    42721 gcttgggcat tcctgaaatg gctgatgttt atgagcgtat cgggaaaatg gagcccgtta
    42781 acctcgatta ctgttacatt cagggattct ccacaattgc ctgcaagcga tacctccaaa
    42841 ttgacaaagc acaacctttg cctgttgctc agtttactgc ctgcaacagt tttttcgatt
    42901 cgccagtaag catcgatttg tcattatcca ttagcgaaaa agagttcgtt attgatgaat
    42961 cgtacgtggt tacagagctt tttgattttc acctgtcgaa tctgttaggc tctttccgtt
    43021 taaccaattg ttatataaaa gcggccaaga gcaattttac ccaggtcagg tttgaagggg
    43081 agcagatatc gtttaaaaat acaatattcg atgtgggtgt taaggatttt caggacacct
    43141 catttggtag tggcgaggcc attttcacta atgttgattt tggcgatggc gatgtttcgt
    43201 tcattaatgc tgggttcaat aattccgatg tcgattttag ggtgtgccgg ttcggcacgg
    43261 gtaaggtcga ttttcactat gcccgatttg gcggaggtgg tgtatcgttt gaacgtgttg
    43321 actttggtag tgggcgagtc gattttcgtg ctgccgaatt ccgtgctggt cgaacctcgt
    43381 ttaaccgtgc ctccttccat gagggggagc tgaactttga gggtgccgaa acgccaaagg
    43441 gtaaactcac tttcaaacgc gcagcatttg gaaacgttac ccttatattc gacttatacc
    43501 agggagcaaa atccgaaatc gtttttgagc gctcgttatt tagccaaaac attagctttt
    43561 ccgaggcaca gcttggtaag ctaattttct ccgattgtca gttcaattct atggttaacc
    43621 tgcatgtaaa gcagagcgac gaaattaact tagcaggctg caccattcgc gatatagttg
    43681 aattttatac ccatggcgat ccacccagaa tcaatagctt aaacctgacg ggtgtacgat
    43741 tgctgggtca aatctatatc gattgggacg ccaatggcgt tcgtagcttg atttactctc
    43801 aggggtgtac tacagttgcc gagaaggctg aacagttccg aattctcaag gaaaacttta
    43861 atgcccttgg aaagtacgac gaggaggatg ctgcctatgt tgaatttaaa cgatgccata
    43921 tgagagcatt gctcttgagc tcaaataagc agggcaagct gaggcattta atcaatagga
    43981 ttatttactg ggcaaagaaa ctgcttttcg attacatggg tctttttgcc acttcgcccc
    44041 agcgagtaat tttcagcatt ttggtggttt acgtaatata cagcctggca tatatcgcta
    44101 taatgggttt gggtttgggg catattattg caacatcggc cagtggagat cagttgggga
    44161 tagttgcccg gggtttttac tttagcgtgg ttaccttttt caccattggc tatggcgatt
    44221 ttgcaccatc aggaatagca aggtttgttg ccggcaccga gggttttatg ggggtatttc
    44281 tgatggctta ctttactgtg gcctttgtga ggaaaatact ccgctaaggg tgtagtgtat
    44341 agtgtgcagt gttcagtttt cagtggtggg tgatagttga tggttgatgg ttgatagttg
    44401 atagttgata gttgatggtt gatggttgac ggttgacggt tgatggttgt aagtaaaaga
    44461 gtgtctattc cgacttattc agagtattcc gagcattccg acccatcccc acttaaaacc
    44521 aagcttttaa cttttttaac ttttcacgtt taccctttac cctttatcct ttatccttta
    44581 tccttatatc ctttatcctt tatcctttat cctttatcct taaaccatct actcatccct
    44641 aagcaggttt g
//
LOCUS       DAOSAL010000014        43369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_666_length_43369_cov_50.426629, whole genome shotgun sequence.
ACCESSION   DAOSAL010000014 DAOSAL010000000
VERSION     DAOSAL010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 43369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43369
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_666_length_43369_cov_50.426629"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            489..1604
                     /locus_tag="PK049_03555"
     CDS             489..1604
                     /locus_tag="PK049_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60666.1"
                     /translation="MRNIVFLSALMVLQHFVYAQEVNDSIIYHQNQRILQFLESSKIS
                     TQIFLGYRYYEEGNGNYNEFVVKRGYITFDKSLNKYLSGRIAPDLTIDKEGDGEGDLE
                     MRLKYCYVELKDEGSYGVFTSPKLLIGEVPTPFIDFEEKVNAYRVVASQYLDDMKILT
                     SADFGVTTSVLLGGKIDEGYQKTVSNAYPGKFGSIAVGVFNGGSYHALEYNNNKTFQW
                     RLSVRPMPERLPGLQFSYAGAHGKGNTALYPDWDTHTGFLSYESRRLVLTGQLFRSLG
                     NHEGTLADSLGNSYTAKGLNAFLEVKFLKNRFSLFGAYGVMRYTDYAGTDIEKTREII
                     GLAYHIYKKNKFVIDLNRSISKGKCKGVIEFMFELAL"
     gene            complement(1683..3353)
                     /locus_tag="PK049_03560"
     CDS             complement(1683..3353)
                     /locus_tag="PK049_03560"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOK60667.1"
                     /translation="MKSDIEIAHEAKMKPITEIAGILGINPDDLIPYGKYKAKIPLSY
                     IDKEKYQQNKLILVSAISPTPAGEGKTTVSIGLAQGLNRIGKQTTVVLREPSLGPVFG
                     IKGGATGGGYSQVLPMEDINLHFTGDFNAIEKAHNLLAALIDNNLQSKKRSLGIDPRT
                     VHWKRVMDMNDRSLRHIIVGLGGTTHGVPRETGFDITAASEVMAILCLAENMEDLKQR
                     LGKIFVGYTYDKKPIYARDLHAEGAMAALLRDAIMPNLVQTIENTPAIIHGGPFANIA
                     QGTNSVLATKMGLSLSEFVVTEAGFGFDLGAEKFIDIKCQYAGLKPSAVVLVATIRAL
                     KYHGGKPLKEVSQPDVEALKKGIGNLQKHVENMKLFGICPVVAINRFTTDTNEEVEYI
                     KSVCREMDVQVAEVDVWGKGGEGAIELAKIVNKIAMNCPYQHKTLYDWNWTPEAKIET
                     LAKKIYGAEAVDYTAQAKEDLKKVYSLGLDKLPICMAKTQKSLSDNPDLLGRPKDFVV
                     TVREIEVAAGAGFIIPIAGQIMRMPGLPDEPAAERIDVGNNGVISGLF"
     gene            complement(3473..4480)
                     /locus_tag="PK049_03565"
     CDS             complement(3473..4480)
                     /locus_tag="PK049_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60668.1"
                     /translation="MRNKWIVAAITVLAIQGCKENRFDSIQPPRDVEPVSITRFEKEL
                     FSINPDSIEKAFPIVYQKHTSFTKLFTEGIIRIGKNDSPDFYRYLGLFLTDTMVQNAN
                     RMVESVFPANAPFEKEISDAFARYSFFFPSKPLPKVYTYISGYNLSLAIDDSLVAVGL
                     DRYLGRNTIQYSLLGIPVYQQRKMHPGKVPSDVMRAWLYSAFPFHDSINNLLSNMIYE
                     GQIMYLTKRMLPYQPDSLIFGFTPQQYKWCLNNESAMWTHLIENKLLFTTNTVDINKM
                     INDAPFTSGFPQESPGRAAVWIGYRIVERFMERNKNIDLQRLMLIKDYQEILTLARYK
                     P"
     gene            4741..4998
                     /locus_tag="PK049_03570"
     CDS             4741..4998
                     /locus_tag="PK049_03570"
                     /inference="COORDINATES: protein motif:HMM:NF015069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family transporter"
                     /protein_id="HOK60669.1"
                     /translation="MIDVAQVFGLLGSITAASLFFPQVYTSWKTKKTHDLAWSGIAIG
                     LLNGVFWIVYGLIRLDPFIYVTNTILFIGAFLLLILKKRYG"
     gene            5244..6731
                     /gene="dnaB"
                     /locus_tag="PK049_03575"
     CDS             5244..6731
                     /gene="dnaB"
                     /locus_tag="PK049_03575"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOK60670.1"
                     /translation="MVSELGKVPPQAVDIEQAVLGAIMVEKDGVIIASEILKPECFYK
                     EAHQIIFKAIQDLSARLEPIDIFTVTEELRKQSKLDEVGGAAYITELSSKVGSAAHLE
                     YHSKIIAQKYIQRELIRVATDIERRSFEDDIDVDELLDYSEMELFKVAEGNIKSDTQP
                     ISNIVVKAFKQIEEAGKREDGLSGVPSGYTDLDRLTSGWQKSDLIIVAARPSMGKTAF
                     VLSMARNMAVEYDVPVAFFSLEMSNIQLVNRLIIAESGIPSEKIRNGRLSNDEWTQLT
                     VKTKQLADAKIFIDDTPALSIFELRAKCRRLKAQHNIGIVIIDYLQLMTGPTDTKGNR
                     EQEVSTISRSLKAIAKELDIPIIALSQLNRSVETRGGNKRPQLSDLRESGAIEQDADV
                     VIFIHRPEYYGFDVDDSGNSTLGIAEIIVAKHRNGAVDDVRLRFQKEMAKFSDLDDMT
                     IIPNTITPTYTSSAPVTFSSKMNNDPVDDFTFNPGKGIDNTNIPF"
     gene            complement(6734..7450)
                     /locus_tag="PK049_03580"
     CDS             complement(6734..7450)
                     /locus_tag="PK049_03580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HOK60671.1"
                     /translation="MILNFFFKHNAYKHIFFDLDRTLWDFEQNMRDALAEIYANHKLD
                     LAFPNPDTFIDCFNRNNHYLWDKYLKGELTKEVLRYKRFEITLAEYGIESKPLAETIG
                     DEYLTIMPLKTALIPGTRETLDYLANRYKLHVISNGFKEVQLPKLQRSNIDKYFEWVV
                     TSEHSGFHKPDKRAFGYALSKANARKGESIMIGDDLEIDILGAKKFGMDQIYFNPKSN
                     PHQAKVTFEVRELVEIKKIL"
     gene            7788..9674
                     /locus_tag="PK049_03585"
     CDS             7788..9674
                     /locus_tag="PK049_03585"
                     /inference="COORDINATES: protein motif:HMM:NF014750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HOK60672.1"
                     /translation="MSTSNPTIKVLMVDAASGFYKVNRFQVGDFFGPIDLGIHLSHKH
                     NSLNIGVGLLAGSIFPGSNRLIVNGISPCWHGFYISSMGGAGLVFDNLGINMFSIVGK
                     AAAPSILYLNRIHGEEIEVELLPVRPRTIWAEGRGGVYSMMQYALNQFGSRYENEPRI
                     LATGPASMYTDFGAIASAPIRKNEITYVDTWAGRGGFGTKMVQQHNIVAIIYGGTYID
                     EDFRDRTVADSWFEDRYQKRLAAKDMEATTKYRFDPKFETGGTFGVNYATMKGDIMAF
                     NYRTIYMTEEERLKIHNDFILNHYLKQFNEETIKTKQQRTCGEPCGAVCKKMHGEFKK
                     DYEPYQTMGPLCGIFDQRAAEKLNHHADMLGFDAISVGGVLSWLMDCMADGLVEPQEL
                     GVADIPVFTPNGFRVVEDSMHNANIGIALLDQMVNPNGVFHLREGARKFARHLARRKG
                     TSVLDKFIYIGFARSGWMVPNQYWTPGVLSPMAIMGKYYMYYGKDFVPPRQLGRENAK
                     RMLNELVMDNLGMCRFHRAWAEDILPDVIEQLYGLKEKFQRSILLTASRINSRNASIF
                     WESERNIDFVYSFLRKKKEVDGASNPELDRWIKYFEEDKRSAALDFWYEIHKGVHESL
                     LEFH"
     gene            9696..10073
                     /locus_tag="PK049_03590"
     CDS             9696..10073
                     /locus_tag="PK049_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60673.1"
                     /translation="MKIIRRILFFIALLLVAIAAFLYWGTYEEGVMAGKIIRISQKGL
                     IFKTYEAKLGLESFGALRGTSPIAETFDFSVEPSNHELVKELEQVALSGERVNIHYKK
                     RYIRVPWRGSTKYFATRVERLQR"
     gene            complement(10128..10502)
                     /locus_tag="PK049_03595"
     CDS             complement(10128..10502)
                     /locus_tag="PK049_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008660659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOK60674.1"
                     /translation="MNIYVSGLSYRINDDDLRQLFEEYGQINSAKVITDRETGRSRGF
                     GFVEMANDEEARTAIEELTGAEYDGKVISVSEAKPKTDKPRNGGGFNRERRGGGYGER
                     RGGYSREGRDSRGGGRGGRRDY"
     gene            complement(10838..12646)
                     /locus_tag="PK049_03600"
     CDS             complement(10838..12646)
                     /locus_tag="PK049_03600"
                     /inference="COORDINATES: protein motif:HMM:NF013050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOK60675.1"
                     /translation="MLKNHPKGLLVAFFSNMGERFGFYTMMAILVLFLQAKYGLSAEE
                     AGGYYSWFYFAIYALALIGGLIADATNRYKTVILLGIIIMFGGYAMIAMPGMSLTMTL
                     LGLFIIAFGNGLFKGNLQAVVGQMYDNPEYSKVRDTGFMIFYMGINVGAFFAPFVATG
                     IRNWFLRSQGFEHDASLPSLCHAFLNGKVTDPESLAKFESLASKVSGATITGTEQLTD
                     FAHKYIDAFSRGYNWAFGVAAVAMVISLLVYVIFNKKLPNKEKKVSTTPAAGSLFTKK
                     NLLNVIVPIALVLLTGAIFWLLGAEIALGLAIGLFFGFVAMMFMMSTKEELPKVTSLL
                     LVFVVVIFFWMSFHQNGLTLTLFARDYTVKEVGPFTNIFFNIWSMLSFIGTIAGLVLM
                     FARKQIRERMIGLVMFLGLGYLTYYQAIKFGASNPIAPEVFQSFNPLFIVVLTFPVMG
                     AFAWMNKRNIEPSTPKKIGIGMIIAAIGFVVVLLSSIHLIAPKTIEAQGLTEWGRVSP
                     YWLISSYLILTIAELFLSPMGLSFVSKVAPQRFQGLMQGGWLLATAIGNKLLFVGSFF
                     WERIELWQLWTIFIVCCLLSAIFIFSIMRRLEEATK"
     gene            13226..14740
                     /locus_tag="PK049_03605"
     CDS             13226..14740
                     /locus_tag="PK049_03605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOK60676.1"
                     /translation="MKVFKTSQIKSIDAYTIENEPIESIDLMERAATAIFNWFRSKYH
                     KNRRIKIFCGPGNNGGDGLALARLLINTGFNAHCYYLKADSYSADFLTNLDRLKELTP
                     ELFLVKHQSDFPEIHTDDLVVDALYGSGLSRSIEGVAAQLIEHINSSGAKVIAIDIPS
                     GLFGEANPVPNNNPVVKSDYCLSLQFPKLAFFMPENEQFLKRWVVLPIGLHTEAIENT
                     ATPYHYTTASSIKTDFFQLSTFAHKGTYGHALVIAGSNGMMGAACLCAKSVVRSGVGL
                     VTMHIPKVGYTIAQVNVPEALTSIDSCETHITQFPELDRYQAVCVGPGIAQSTETDQM
                     LVKLIKTCQLPLVVDADGLNLLSRNIDVLNSVNNALILTPHPGEFDRLFGKSQCSWER
                     LERAREMAQKYGVIIVLKGAYTRVVLPSGNVHFNSTGNPGMATGGSGDVLSGIITGLL
                     ARGLPPADAAITGVYIHGLAGDLAADEFGQISLCASDIVNYICKAFQHITNNDE"
     gene            14730..15830
                     /locus_tag="PK049_03610"
     CDS             14730..15830
                     /locus_tag="PK049_03610"
                     /inference="COORDINATES: protein motif:HMM:NF027441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4831 family protein"
                     /protein_id="HOK60677.1"
                     /translation="MMSKNLFLAVLSLVLLGCNSKTIQVEPAGKTIDYKKVAVYYSLP
                     RTVVYVKVVSSQSEYFPGPYADYAKKFLGIDGVSNTEKVEYLIEKIDVETTAEPDPKA
                     VFAIYPSGKGYGNFINLSNEGLILPVNQTPESRAIAKNKEVSSKGEVIYKDLSVNPFV
                     AEETTTFYGRTLRDSVYVRVPIQRSMTIERNLEEKAKEAADFIFSIRKKRLDFLTPDI
                     DHPFSGDALKLILKELQRLEDEYLALFVGKRFTHTFTQTYSFIPSNTEGESAILFRFS
                     ESKGLLSSTDLSGRPILIEFDQPEYPENIDATKNSLETIALKAKIDRFYYRIPVAVNM
                     RVIDGKQLLVEKRLSIFQYGLLAQLPVNFPGK"
     gene            complement(15831..16385)
                     /locus_tag="PK049_03615"
     CDS             complement(15831..16385)
                     /locus_tag="PK049_03615"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HOK60678.1"
                     /translation="MKNRIWVLTIWMLGISASVSIAQETKINWIEIDRAFEVNEKTPR
                     KIFIDVYTDWCGWCKKMDENTFTNPVIASYLTQKFHAVKFNAEGSKDITYKGKVYKNK
                     GQGQGQRNPHEFAIAILQGRLSYPSIVFMDENNNLITYIAGYLTPEQLEPIMAYIESD
                     AYKTQKWEDFRAKFVSKLNTVANP"
     gene            complement(16436..16963)
                     /locus_tag="PK049_03620"
     CDS             complement(16436..16963)
                     /locus_tag="PK049_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HOK60679.1"
                     /translation="MAIIKPLRGITPKIGKNCFIAETAVIIGDVEIGDDCSIWYGAVL
                     RGDVNSIRIGNRVNIQDNAVLHTTYQKTTIEVGDDVSIGHGVILHGAQVSRGALLGMG
                     CTVMDNAKVGEGAIVAANALVLENTVVEPACVYAGVPARFVKKVAPELSRETNERIAR
                     NYLFYSGWYKDGDAK"
     gene            17034..17759
                     /locus_tag="PK049_03625"
     CDS             17034..17759
                     /locus_tag="PK049_03625"
                     /inference="COORDINATES: protein motif:HMM:NF014037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HOK60680.1"
                     /translation="MNIRVYGDLNDFLPQGKKHKDQRVGFFGMPTVKDLVESIGVPHV
                     EVFLILADSLPVKFDYRPANGTLISCYPKFTSLPLSAIALRPDFEKPPRFILDVHLGR
                     LAAYLRFCGIDTLYDKSFNDNQILDIAQADGRIILTRDKGILRNGQAQFGYFLRNTNP
                     QLQLAEVVHYFDLAHYLDPFSRCSLCNGEVEQVDKSMVKNLVPSTTYQIYSEFFQCSR
                     CGQVYWEGPHFIRISKLLSRYSK"
     gene            17943..19796
                     /locus_tag="PK049_03630"
     CDS             17943..19796
                     /locus_tag="PK049_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOK60681.1"
                     /translation="MDKQKSVQELDEVVVRFSGDSGDGMQLTGTLFADASALFGNDVS
                     TFPDYPSEIRAPQGTIGGVSGFQVHFGNRKVNTPGDFCDVLIAMNPAALKANAKWVKR
                     GGMIIVDADQFNEKGYQRAGYKTFDAVSELNLQDYKVIFAPITNLTKESLKDSGLDNK
                     SIIRSKNMFALGMVYYIFDRPLNFTEKFFEKKFSKKPEVVEANKKVLRDGFNYAANIQ
                     AIASTYKVKQATTLPKGKYRNINGNTATAWGLIAAAEKAGLPLFCGSYPITPATEILI
                     ELAKRKDLGVKTLQAEDEIAGICTAIGAAYAGNFAVTTTSGPGLSLKSEAIGLAVMTE
                     LPLVIVDVQRGGPSTGLPTKTEQSDLMQVLWGRNGEAPVVVIAASTPSNCFDYAFMAG
                     KIAMEHMTPVVLLTDGYIANGSEPWRIPSMKDYPEIKPPIVPEGTEGYMPYARDEKRL
                     ARGWAFPGKKGIAHRVGGLEKDFLKGTVSHDPTNHQRMVDIRAAKVQRVQEFIPEQQV
                     IGDPEGDLLVIGWGGTFGHLFDAVQEMRANGKKVSLCHFNYINPMPKNVADIFGRFKK
                     LVVCELNMGQFANHLRMNYQQFNYLQYNKVQGLPFTVVELVEKFNQILEGK"
     gene            19799..20827
                     /locus_tag="PK049_03635"
     CDS             19799..20827
                     /locus_tag="PK049_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HOK60682.1"
                     /translation="MEEQYMNYTPQDFKSDQEVRWCPGCGDHAILNAVQKALPEVSKQ
                     LKYNLERYVFVSGIGCSSRFPYYVNTFGFHGIHGRASAIATGVKVANPTLSVWQVTGD
                     GDALAIGGNHFIHAVRRNIDINILLFNNQIYGLTKGQYSPTSPLGAITKTSPFGTVEH
                     PCRPGELVIGAQGKFFARSLDVDVKLTAEIMVEAAKHDGTSVVEILQNCVIFNDGAYD
                     YITDREHRDDRVIVLRHGEKMVFGKNRDKGLILSGLSLKVVTIGQGGFTMDDILVHDA
                     YNPNPGVHMMLANMEYPDYPVALGVIRNVKDSTYDDNVRDQVLEVMKTAKIKSVDDLL
                     NSGDTWEV"
     gene            20989..23463
                     /locus_tag="PK049_03640"
     CDS             20989..23463
                     /locus_tag="PK049_03640"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOK60683.1"
                     /translation="MVKQKFIKALIALQFILSFSVSEVKAQDSFKQIFSDAEYYFFLQ
                     DFKEALPLYQSLYQQDSTNANILYKLGMCYLNIPGLKQNAIPYLEKASKNVNPKYREG
                     YYRETAAPIQTYFYLGQAYMVNFKFDDALLAFQKFKDNIDVKDVYNLDYVNQQIKACE
                     VARNFISRPIVMKTEHIDLFPDRNKNCNYPVISGDRQTMVFTVKEKFYTAVYYSRWID
                     GKWSSPRNITLDLRVEGELYTTALNYTGDYLILFVNEVTSGNLYYSTLVGDKWQPVKK
                     LPAPINSKDWETFASLSVDGKQMYFVSNRKGGYGGTDIYMSSLQPDGKWSNPINLGPQ
                     VNTPYNEESPIVCPDGRTLYFASQGHNSMGGFDIFYSRKIDGNSWSMPINLGYPFNTP
                     DDEFYFYPLDSLSGVMPMAISNQSTFYELYKVNIYPSISRKIELFGKVNLSDNADIKS
                     DSIAILVKDTANNLIAMALPLSDGTYSVAIKPGRYSLEATSQFYVMNPLQLHIPTTYN
                     QEKYLLDINLDAKPITKEEVIRFNYVLFDFDSYELKRDAQFELEKVYKLMTDYPDLYI
                     EVIGHTDSKGSPMYNLMLSARRANAVAEYLVNKGIDEKRFVVRGMGSLVSFAANTNPD
                     GSDNPNGRKLNRRASIRVFNPNKNLKIEFVDVPEHLKPQTQNYTIMLAPIDDTISPDL
                     IKAIEKKFNINLREFVIGSRRLVCMNVYKSKADAIEHLNTIIDMGASRAVLVNEVELQ
                     RLVAALQKNLITKQSVFTILVATSEIPLTLDFFRGLYVTEEVGNDGLYRYYFGAFNEK
                     AKATEMLEKVNSMGFPNAILVKLEKR"
     gene            23463..24029
                     /locus_tag="PK049_03645"
     CDS             23463..24029
                     /locus_tag="PK049_03645"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOK60684.1"
                     /translation="MEGNVLENSVVKLRAPEPEDVDLLYSWENNMEIWKVSNTLTPFS
                     KYLLKKYIETSHLDIWESKQLRLIIEAKDQNSLMNVPVGLVDLFDFDPYHLRAGVGVL
                     IAKNEDRRKGYATEALNVLIRYVFEVLQLNQLYANVSASNVISLRLFENLGFTKVGVK
                     KDWLKTLNGWEDEVLLQLLNPKPRLPKS"
     gene            complement(24057..25517)
                     /locus_tag="PK049_03650"
     CDS             complement(24057..25517)
                     /locus_tag="PK049_03650"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOK60685.1"
                     /translation="MSNLEKYFEQFRKNIVGIDATYKTIYGEQKLIYADWIASGRLYR
                     PIEEKMLHLFGPMVANTHTESSFTGKFMTEAYHYALKEIKRHVNAGPEDVIVTSGFGM
                     TSSINKFQRILGLKNKSFVFKNPKERPVVFVTHMEHHSNQTSWYETIAEVVVVEPDKD
                     LLVSPQKLRETLERYNDRPMKIGSFSACSNVTGIKTPYHELAKIMHGAGGLCFVDFAA
                     SAPYVKIDMHPADPMERLDAIFFSPHKFLGGPGASGVLIFNSNLYKNRVPDQPGGGTV
                     DWTNPWGEYKYIDDIERREDGGTPGFLQAIRSALAIRLKEQMGVENIEKREEELVHLA
                     LERLSKIKGLHILADNCLNRIGAISFYIDNLHYNLIVTLLSERYGIQMRGGCVCAGTY
                     GHFLLNVSKEQSHRITDLINKGDLSEKPGWVRWSLHPTTTNAEVEYIANALAEIVAYG
                     KEWQNEYSYCKHTNEFCNKNPEAAMLWKNVEEHFQF"
     gene            complement(25525..25989)
                     /locus_tag="PK049_03655"
     CDS             complement(25525..25989)
                     /locus_tag="PK049_03655"
                     /inference="COORDINATES: protein motif:HMM:NF024976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HOK60686.1"
                     /translation="MSTANGGSLNFPAYQFRVKQENGNRYIFDVIRKKFIALTPEEWV
                     RQHLLSYLITDLNLPVGLIGVEVSFSFNGVNHRADVVVYNRKGEPILLAECKAPNVEI
                     TNEVVDQICRYNYALNAEYLLVTNGLKHLILWLSEGKGWITLDGFPELRNKI"
     gene            26201..26971
                     /locus_tag="PK049_03660"
     CDS             26201..26971
                     /locus_tag="PK049_03660"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HOK60687.1"
                     /translation="MKTKEEIVENWLPRYTGQALEDFGSHILLTNFRGYLEFFANYFN
                     VPIVNPNANMPCATANGITMIDFGMGSPNAATIMDLLSAVKPKAVLFLGKCGGLKKKN
                     NVGDFILPIAAIRHEGTSNDYMPPEVPALPAFTLQRVVSSTIRNHNRDYWTGTVVTTN
                     RRVWEYDDNFKEYLRRTRAMAIDMETATIFTVGFYNEIPTGALLLVTDQPMISEGVKT
                     AESDQKVNSLFVEEQIKIGIESLTRLINNSESVKHLRF"
     gene            26981..28027
                     /gene="holA"
                     /locus_tag="PK049_03665"
     CDS             26981..28027
                     /gene="holA"
                     /locus_tag="PK049_03665"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOK60688.1"
                     /translation="MAKQSQTVETFQNILQELKNKIYKPIYFLTGDEPYFIDKIAEYI
                     EDNVLSSSEKAFNQTVVYGRDISAADIINAARRFPMMSNYQVVIVKEAQNVRNLSDLE
                     IYLKTPLNSTILVVCYKIASDGKKSSDKFSGVVKLAKQKGIYFESKKFYDYQIPDWIV
                     GYLKDKGIKIDPLAANLLTEFVGNDLSRLAHELDKLAIILPADSKQIQATDIERNIGI
                     SRDFNRFELTKALGEMNFARAMRIADHFARNPNANPFVLTITAIYQYFIKILKYHFLA
                     DKSKASVAKELGVNEFFVAEYERAAKRYSPSRCVSIIHWLSEYDMRSKGVNNGSTDHG
                     ELLRELIYLIVFGK"
     gene            28024..28638
                     /locus_tag="PK049_03670"
     CDS             28024..28638
                     /locus_tag="PK049_03670"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOK60689.1"
                     /translation="MTYIIIAITAIVSFAAFGNSILFQQLLLNPYIVFKRNEWYRLIS
                     HAFVHANFLHLFVNMIVLLSFGRYVESILKQLQSIGSIGMHQVHFLILYLGAAIVSSL
                     TTLRKHKNDPYYQSVGASGAVSAIVFFSIFFSPLEKLYLMAIIPIPGIVFAIAYLFYS
                     NYMSRKGGDNINHDAHFVGAVFGFIYPILIDPKLFFFFLKQLGL"
     gene            28649..29077
                     /locus_tag="PK049_03675"
     CDS             28649..29077
                     /locus_tag="PK049_03675"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOK60690.1"
                     /translation="MQAEAQITIHPDGLDASVLQLVAIVSYMNGNWLWVRNKNRDTWE
                     LPGGHIEPGELPVEAANRELFEETGAIRYSIKQLFDYTVRLDGAVSYGRMFYAEITEL
                     ETLPNSEIGEVKAFKSIPENLTYKRVQPRLFEMAIEYLKG"
     gene            29093..29956
                     /locus_tag="PK049_03680"
     CDS             29093..29956
                     /locus_tag="PK049_03680"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOK60691.1"
                     /translation="MTQNAYIWYNGEFHRADSMVLAVSNRSFRYGDGLFETIHCYGTE
                     PYHLELHYERLTKGLQILEIEKPSSISKVKLGEVITKLLNKNRHFGSTRVRVTVFRNG
                     EGLYTPESSDASIIVESQPLDFKFYAYNQQGLIVDVYPDIKKSVNILSTVKSCNALLY
                     VKAGLYRKRNLLDECIILNEFGRVAETISSNVFVVKRGEIFTPGLTEGCIPGIMRRVV
                     INLARSLGYKVFDDVALNTKVFSTCDEFFITNAISGIRWVLGLGSNRYFNTVSRHLSI
                     ELNKATFKDLV"
     gene            complement(29948..30547)
                     /locus_tag="PK049_03685"
     CDS             complement(29948..30547)
                     /locus_tag="PK049_03685"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HOK60692.1"
                     /translation="MAKGKEIDINLFDIKFKQIPPKSGRVLIAQPSLNDPFFKRTVIY
                     LVEHDANGCMGFIVNRKLNISLSDILVDFPDVDVNVSVGGPVSPETINFLHTFGDLIP
                     NSYPLGNGIYMNGDINALKSLAIAGKVNAKNLRIFIGYSGWGPKQLENEIKEKSWVVA
                     NFAPTDVMKGVYDNWYFAVQQLGEKFKVWTIYPENPSLN"
     gene            30738..32231
                     /gene="nhaC"
                     /locus_tag="PK049_03690"
     CDS             30738..32231
                     /gene="nhaC"
                     /locus_tag="PK049_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HOK60693.1"
                     /translation="MIKQPKLIHGIIPIAFLILFLTLNVIYFGDETLSGANQIALLLA
                     STIGSAIAIHLGYKWSYIRQIIVKTIGSAMPSILILMLIGALAGTWMISGVVPTLIYY
                     GLKILHPKIFLFATVVITALVSLATGSSWSTVATMGVALLGIGNALGFNHAIIAGAIV
                     SGAYFGDKMSPLSDTTNLAPAMAGTDLFTHVKYMTITTIPSLAITLIIFLVIGLRFEP
                     QGVVDVQTTMNTLTSTFNISPWLLLVPIFLIVVIVKKTPPLPSIMFGVLLGGVFAIIF
                     QPHIVEQVSGVTDNYPEAGYIAVMKSMFGKVTINSGDAQMNELLTTRGMAGMMNTIWL
                     IITAMIFGGVMESAGLLVKITETIIRFAKSTASLVASVVTSCLFFNVTASDQYISIVV
                     PGRMFAKTFRKRGYKPELLSRTLEDSGTVTSVLIPWNTCGATQAAVLGVPTFSYAPYA
                     FFNIISPFMSVFIATIGFKIRRFTPEEMQEIEREDENSPVELKFEQE"
     gene            complement(32268..34679)
                     /locus_tag="PK049_03695"
     CDS             complement(32268..34679)
                     /locus_tag="PK049_03695"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOK60694.1"
                     /translation="MSTTKIKFSKEEILNDYYIAHLSRNISLMGRKEVLTGKAKFGIF
                     GDGKELAQIALAKTFKEGDWRSGYYRDQTFMMAAGLFTAKEFFAQLYGQTDIELNPGN
                     AGRTFNNHFATRSLNPDGTWKDLTKQKNSTPDISPTAGQMPRLLGLAWASKLFRNNKE
                     LWRFEQFSRKGNEVAFGTIGDASTSEGHFWETLNAAAVLQVPMVVAVWDDGYGISVPR
                     DYQTAKGNISELVKGFEKGESDVNGIYIYRVKGWDYPALIETFTQGVERSRTEQVPVL
                     FHVTEITQPQGHSTSGSHERYKSPERLQWEKDYDCNARFRNWIVTEGYATETELNEIE
                     AKAQADAKAAREKAWAEFTNPIKKERDELIKIIDNRSCFCKREHIDKVGEITEQLKAI
                     PNPIRKDNISAAKRILRHVCSDCSIREKLQADLTAWIERNMNDGYDRYSSHLYCETER
                     AAWKVKGIPPVFKDDSPLVAGREILRDNWDAQFQKNPLLVIFGEDVGGIGGVNQSYEG
                     LQKKYGEIRISDTGIRENTILGQGLGLALRGMRPVAEIQYFDYLLYALQTMSDDLATI
                     RWRTKSGQMAPLIISTRGHRLEGVWHAGSPLSMVINSIRGVYVCVPRNMTQAAGFYNT
                     LLEADDPALVIEPLNGYRLKEKRPENIGEYKIPLGVPEILREGSDVTLVTYGSCVRIA
                     QEAVEQLWEFGISVELIDVQTLLPFDLPNLILDSIKKTNRIVFFDEDVPGGATAYMMQ
                     KVLEERAGYQYLDSPPKTITAREHRPAYTTDGDYFSNPNAEDVFDGIYELMHEVNPKR
                     YPKLF"
     gene            34860..35105
                     /locus_tag="PK049_03700"
     CDS             34860..35105
                     /locus_tag="PK049_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60695.1"
                     /translation="MLTLKLILVAATILGIALLGMAVRIIFHKSHKFPETSAGHNKEL
                     RKRGVTCPRHEEIKCWGKNGKNTDCATCYEHAREVVN"
     gene            35117..35299
                     /locus_tag="PK049_03705"
     CDS             35117..35299
                     /locus_tag="PK049_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60696.1"
                     /translation="MALLKLIGVVAFLLGIVIIGLSLKTIIKSRKSGCNDSDIGYSCG
                     CGGGTNCSTNAASKGI"
     gene            complement(35300..36295)
                     /locus_tag="PK049_03710"
     CDS             complement(35300..36295)
                     /locus_tag="PK049_03710"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOK60697.1"
                     /translation="MRYLTVFTLAVIILVSCSPKKEYLKIEGFTQGTTYSITYYDSLI
                     RDFSVQIDSILGRIDHSMSIYNDSSIISLLNSNISDSIDDMLKEVIVSSFEIYHQSDG
                     AFDITVGPVVRAIGFGKDKTQGIDSAKVKQLLPLIGMNKIHLDGNTLKKDDSRIQIDV
                     NAIAQGYTVDVIGKYLESNGIKHYLVEVGGEITARGKNSKGTDWVVGIDKPVESALPG
                     ENIQAKISISNGYGLATSGNYRKFIEVNGQKYAHTINPKTGFPELNSLLSATVVAPSA
                     TKADALATAFMVKGVEWSKNFVVKSNDIDAYLVYSDSLGNYLVWMNEGMKKRIIP"
     gene            36386..39376
                     /locus_tag="PK049_03715"
     CDS             36386..39376
                     /locus_tag="PK049_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOK60698.1"
                     /translation="MLNLLGDQAYLNSYQLDIYDTSFDKLMQKRIYKVLLICSSYDAF
                     LLEEDGRIDEQIFNEYVSLNLRYPPVFLQAHSAREVLRILQEEHIDLIISMLNVGEMD
                     TFNLAKMLKQRYPSIPIVVLTPFSREVSLRLQNEDLSAIDYVFCWLGNADLLLAIIKL
                     IEDKMNAEHDILEIGVQAIILVEDSVRYYSSFLPNLYKIIFRQARMFMTEALNEHHRM
                     LRMRGRPKILLATTYDEAIELYSKYKNNVLGVISDISYKVNNKRDHVAKAGLQLCRHI
                     KADDQNIPFLLQSSELSNKIYADELCVGFLHKYSKTFSLDLRDYIVNNFGFGDFVFRD
                     PDTLAEIARASDLRQLQKLILTIPDRSLVYHSSRNDISKWLNARAIFSIAQLFKPMRL
                     KDFRSIDDARNFIYYAISAYRLSKGRSIIAKFDRETFDEYKFFSRIGDGSIGGKARGL
                     AFVNQVIRNYNLFNKFENTIITIPRTVVLSTDIFDEFMESNNLYSIALQDLDDEVILE
                     AFLKAKLPERIKIDLRKVISVFQRPLAVRSSSKLEDSHYQPFAGIYSTYMVPHSDNPD
                     QMLQHLAKAVKAVYASVFYKASKAYMNATQNVIDEEKMGVIIQEICGNQYGNMYLPTL
                     SGVARSINFYPIGSEKTEDGIVSVGFGLGKLIVDGGVALRFSPKYPKKILQLSSVEMA
                     LRQTQKNFYALNVDPNSFEPKVDDSSNLLNLDIREASSIPDFRLITSTYDMHSGVLRD
                     GYYDEGLKLVTFAPILKHDTFPLAKILSELLEIGEREMNNHIEIEFAANLNPPSGMPR
                     LFNFLQIRPIVDSDQTELVEWNEMDINKAILYSKSALGHGKVKDIYDFVYIKPESFNP
                     AMTKEIAHELDELNKRFIDLQRNYVLVGPGRWGSSDPWLGVPIKWSQISQARVIVEAG
                     LENFRVDPSQGTHFFQNLTSFRVAYLTVNPYLHDGSYDIQFLNSIPAWFETKYIRCIR
                     FEQPLVIQVDAKTNQGVILKPENG"
     gene            39546..40883
                     /gene="gdhA"
                     /locus_tag="PK049_03720"
     CDS             39546..40883
                     /gene="gdhA"
                     /locus_tag="PK049_03720"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019149313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOK60699.1"
                     /translation="MNVNKLMAELEAKHPGEVEYLQAVREVLESIEEVYNQNPQFESA
                     KIIERMVEPDRIFTFKVPWMDDEGNIHVNLGYRVQFNNAIGPYKGGLRFHPSVNLSIL
                     KFLGFEQIFKNALTTLPMGGAKGGSDFNPKGRSDAEIMRFCQSFMLELWKHIGPETDV
                     PAGDIGVGGREIGYLYGMYKKLAGENTGVLTGKGINWGGSLIRPQATGFGATYFAQEM
                     LATKGMSFAGKRVAISGFGNVAWGAALKVTELGGKVVTISGPDGYILDEAGISGEKID
                     YMLELRASNQDIVEPFAKKFPGAKFFAGKRPWEVKVDVAMPCATQNELGKADAEALVK
                     NGVVCVAEGANMPCTPEAIEVFQHNKILFAPGKAVNAGGVATSGLEMTQNSMKLNWPS
                     EEVDARLHQIMKSIHEACVKHGTEKDGYVNYVKGANIAGFLKVANAMLDQGLI"
     gene            40952..41365
                     /locus_tag="PK049_03725"
     CDS             40952..41365
                     /locus_tag="PK049_03725"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOK60700.1"
                     /translation="MSTTSTIIYKGDLRTEATHTRSGVTITTDAPEDNQGKGECFSPT
                     DLLATSLGCCMVTIMGIAARTHGFNIDGTKIDVQKVMGTNPRRVVEVIIDLYFPHNNY
                     TAKERKLLEASAKECPVAQSLHPDLKQTIRFHFPE"
     gene            complement(41395..42279)
                     /gene="fabD"
                     /locus_tag="PK049_03730"
     CDS             complement(41395..42279)
                     /gene="fabD"
                     /locus_tag="PK049_03730"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOK60701.1"
                     /translation="MKAYVFPGQGAQFVGMGKDLYDSSSVAKELFEKANEILGFRITD
                     LMFAGTDEDLKQTKVTQPAIFLHSVILAKVLGNDFKPEMVAGHSLGEFSALVAAGALS
                     FEDGLRLVSARAMAMQKACELQPSTMAAVLGLDDAKVEEICASINEVVVAANYNCPGQ
                     LVISGSVKGVDMACEKLKEAGAKRALKLAVSGAFHSPLMEPARQELEAAINATPFKKP
                     VCPIYQNATAKPTSSPEEIKKNLVAQLTAPVRWTQTVQNMIADGATEFVELGPGSVLQ
                     GLIKKVNKDISAESKQSL"
     gene            complement(42389..42985)
                     /locus_tag="PK049_03735"
     CDS             complement(42389..42985)
                     /locus_tag="PK049_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60702.1"
                     /translation="MGISKIDKDLYTRIVRNVNAQLGKYITFNKNCSVEPIEQTFFPN
                     SKLLSITDYDCIPASKLMVVDNGVQVYPLSGLGQWNSLLMNERLNLNIKNVLDYLKLY
                     YSTYLFPYQYQKIVETVNDLDFIEFPELELFDVISHQIQAPVVTLSNNQFTIYCNITA
                     DRSLYNAAFDIYPDGKVLLLDKKVVLQDLPIGEMVIDL"
     gene            43116..>43369
                     /locus_tag="PK049_03740"
     CDS             43116..>43369
                     /locus_tag="PK049_03740"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOK60703.1"
                     /translation="MKKLLVILTILATNFYTANAQLFKVFGHDVGFIYVGPKVGMNFS
                     KFSQWSYTGYDVKNRIGYQLGVVGDFGFTNRFSFQTELNF"
BASE COUNT        12272 a         9035 c         9359 g        12703 t
ORIGIN      
        1 acaagaaaga acgtgaaaat cactctttgc gatgtttttc ctttgcgatg caaagtttca
       61 gtgttgctct ttggaaaatg tattacgtgc atcgctatac atgtgggaat aatcatagtg
      121 ttacaaatat tacgcccctg cggggctgga aacggtgttc gttgctcgtg aaactattgt
      181 catgttgagc gttagcgaaa catctcatta cacctaatgt ggcagccaac tttgctagct
      241 ctatccgtca tattcttgtt gggcttaact gttcactttc cctctaactt cttctaactc
      301 agtcatattc ttttgtttcc gaatcattcc gaatcattcc gaatcattcc gaatcattcc
      361 gaatcattcc gactaattcc tctaaattca tgttacttgg gataaactta acattaactt
      421 aacataccgt ttaaacctta acatatagtt tacatagacg taactatctt tgcacaaaat
      481 ttttagcaat gagaaatatt gttttcctat cggctttaat ggttttgcaa cattttgttt
      541 atgcgcaaga ggtaaacgac tcaataattt accatcaaaa tcaaaggata ttgcagtttt
      601 tggaaagcag taagatttca acccaaattt ttcttggcta caggtattat gaggaaggca
      661 atggaaacta taacgagttt gtcgtaaaaa ggggatatat cacctttgat aagtcattga
      721 acaagtatct atccgggcgc atagctcccg acctaacaat tgacaaggaa ggcgatggtg
      781 aaggcgacct tgaaatgcgc ttaaagtatt gttatgttga gcttaaagac gagggcagtt
      841 atggggtatt cacaagccct aaactattaa ttggagaggt tcctacccct tttatcgatt
      901 ttgaggaaaa ggttaacgcg tacagggttg ttgctagcca gtatctcgat gacatgaaaa
      961 tcctcacatc ggccgatttc ggggttacca catcggtttt actgggtgga aagattgatg
     1021 agggttacca aaaaactgtc agcaatgctt atcccggcaa atttgggtca attgctgtgg
     1081 gggtttttaa cggaggtagt taccatgctc ttgaatacaa taacaataaa acttttcagt
     1141 ggaggttaag tgtacggcca atgcctgagc gtttacctgg tttacagttt tcctatgcag
     1201 gagcacatgg taagggtaat acagcactct accccgattg ggatacacat acgggtttct
     1261 tgagctatga atctcgcaga cttgttctta cagggcagct attccgttcg ctaggaaacc
     1321 atgagggtac actggccgat tctttgggca actcatacac agctaagggt ttgaatgctt
     1381 tcctagaagt aaagttcttg aaaaaccgct tcagcttgtt tggtgcttac ggtgttatgc
     1441 gttatactga ctatgccgga acagatattg agaaaactcg cgagattatt ggattagcct
     1501 atcatatcta caaaaagaat aagtttgtta ttgatttaaa ccgaagtatt tccaaaggaa
     1561 aatgtaaggg ggttattgaa tttatgtttg agttggcttt gtagtaatta ctttccgttt
     1621 caacatagta agtataaaaa aaggttggca aagcgccaac ctttttttgg ctaaaggtga
     1681 ggttagaata gtccgctgat aactccattg ttacccacat caatacgctc ggctgcaggt
     1741 tcatcgggta atccgggcat acgcattatc tgtccggcaa ttggaatgat aaatcctgcg
     1801 ccagcagcaa cctcaatttc cctaacagta accacaaaat cctttggtct gccaagcagg
     1861 tcaggattat cggagagcga tttttgggtc tttgccatac aaattggcag cttgtcgagc
     1921 cctagtgagt ataccttctt taaatcctcc tttgcttgag cggtgtaatc tacagcttca
     1981 gcaccgtata tcttcttggc aagggtttcg atttttgctt cgggtgtcca gttccagtcg
     2041 taaagggttt tatgctggta tgggcaattc atggcaatct tgttaacaat tttagccagt
     2101 tctatggcac cctcgccacc tttaccccac acatcaacct ctgcaacctg aacatccatt
     2161 tccctgcaaa cgctttttat gtattcaacc tcttcgttgg tgtcagttgt aaacctattt
     2221 atggctacca cgggacagat gccaaataac ttcatgtttt ccacatgctt ttgcaggttg
     2281 ccaataccct ttttaagagc ctctacatcg ggttgtgaaa cctccttaag tggcttcccg
     2341 ccatggtatt tgagagccct tattgtggct acaagtacaa ctgcacttgg ttttaaccct
     2401 gcatactggc acttgatatc aatgaatttt tcggctccca gatcgaaacc aaaaccagcc
     2461 tcggtaacca cgaactcact gagcgagagt cccatttttg tggctaaaac cgaattggtt
     2521 ccctgggcta tattggcgaa tggtccgcca tgaataatgg caggggtatt ctcaatggtt
     2581 tgcactaggt taggcattat ggcatcgcgt aaaagcgcag ccatggcacc ctcggcatgt
     2641 aaatcgcgag catagatggg ctttttatcg taggtatagc ccacaaagat tttccctaac
     2701 ctctgcttca ggtcttccat gttttcggct aggcagagta ttgccataac ctccgaagca
     2761 gctgttatat caaatccggt ttcgcggggt accccatggg ttgtaccacc cagcccaaca
     2821 atgatgtggc gcaacgatct gtcgttcatg tccatcactc gcttccagtg cacagtacgg
     2881 ggatcgatgc caagggaacg ctttttgctt tgtaaattat tgtctattag cgcagcaaga
     2941 aggttgtgtg ccttttcaat ggcgttgaaa tcgccagtaa aatgaaggtt gatatcttcc
     3001 atgggaagaa cctgtgagta tccaccaccg gttgctcctc cttttatacc gaacacaggg
     3061 cctagcgagg gttcacggag aaccactgtg gtttgtttac caattcggtt aaggccttgc
     3121 gccaagccaa ttgatactgt tgttttcccc tctccggcag gtgttggtga aatggccgat
     3181 accagtatca acttgttttg ctgatacttt tccttgtcga tgtagctcaa gggaattttg
     3241 gctttatact taccgtaagg tattaaatcg tcaggattga tgcccagaat tccggctatc
     3301 tcggttatgg gcttcatttt agcttcgtgt gcaatttcaa tatcgctttt catagtaaaa
     3361 atgatttagg ttattcaggg aaagtgttat taacttatgt agatttcatc atacaagttt
     3421 taccaattat gaataacaag ttacagcaaa atttgtttat gggcaacttt gcttacggtt
     3481 tatatcttgc cagggtaagg atttcctgat aatcctttat aagcattagc ctctgaagat
     3541 cgatattttt gtttcgctcc ataaagcgct caacaattcg gtagcctatc caaactgcag
     3601 cccttccggg cgattcctgt ggaaaccccg atgtaaatgg agcatcgttt atcatcttgt
     3661 tgatgtcaac agtattggtg gtaaagagca gcttattttc aataaggtga gtccacatgg
     3721 cagactcgtt atttaggcac cacttgtact gctgtggtgt aaaaccaaaa attagggagt
     3781 cgggttgata tgggagcatc cgcttggtta ggtacatgat ttgcccttcg taaatcatgt
     3841 tactcagtag gttgttgatt gaatcgtgga aagggaaagc gctgtacagc caggcccgca
     3901 taacatccga tggaacttta ccggggtgca tcttacgttg ctggtaaaca ggtatgccaa
     3961 gcagggaata ctgtatggta tttctaccca aatagcggtc gagccccacg gccaccaaac
     4021 tatcatctat tgccaatgat aggttataac ctgaaatata ggtgtaaacc tttggtagtg
     4081 gtttgcttgg gaaaaagaaa gagtacctcg cgaacgcatc gctaatctcc ttttcaaatg
     4141 gagcattggc tgggaataca ctctcaacca ttctgttggc gttttgtacc atggtatcgg
     4201 tgaggaataa ccctaagtac ctgtaaaaat cgggggaatc gttcttccca attcgtataa
     4261 tgccctcagt aaacagtttt gtgaaacttg tgtgcttttg ataaacaata gggaaggctt
     4321 tttcaatgct gtcaggatta atactaaaaa gctctttctc gaacctggta atggaaacag
     4381 gttcaacatc tctagggggt tgaattgagt cgaatcggtt ttccttacag ccctgaatgg
     4441 ccaaaacggt aatggctgct actatccatt tgttcctcat ggtgtttaag tttttaaaag
     4501 cagaacaaag atagtaaata catggataaa ggataaagga taaaggataa aggataaagg
     4561 ataaagaatg tctagtcctg aaataagtga cccgtcctga aaaatgtcta cataaaattt
     4621 tgtgaataaa aaacgatttg ataaaacttc tttaccattt gatggctatc tttgaatgga
     4681 ttatagtcaa ttgagttagg actgtaaagc atttggggtt taatgcttaa tttttaagct
     4741 atgattgatg ttgctcaggt atttggtttg ctcggttcaa taactgcggc aagcttattc
     4801 tttccacaag tttatacttc atggaaaacc aaaaagaccc acgatttggc ttggtctgga
     4861 attgctattg gtctgctgaa cggagttttt tggattgttt atggcctaat ccgtttagat
     4921 cccttcatat acgtaacaaa cacaatacta ttcattggcg cattcctgtt gcttattctt
     4981 aagaaacgtt acggttaatc taggctcagt gaccgcagtt tattcactac attttctatt
     5041 actttcctat tgtattgaga atttgtgggt ttaccccatc ggttaataac ttgttagtaa
     5101 ccgatgtttg aatgtgaaaa aacactatgg gatgcagctt ccgtttcgcg aattgaaatt
     5161 ctacctttgc tttgcaaaaa caaataaaaa tggccggaga gagaaaaagt acgggaaaaa
     5221 agccatattc accagctaaa ccaatggtat cggagctagg taaggttccc ccgcaagcgg
     5281 ttgatattga acaggcagta cttggcgcta taatggttga aaaggatggt gtaattattg
     5341 caagcgagat ccttaagccc gaatgtttct ataaggaagc acaccaaatt atctttaaag
     5401 ccattcagga tttatcggca aggcttgaac caatcgatat tttcacggta accgaggagt
     5461 tgcgtaaaca gtccaagctc gatgaggttg gcggggcagc gtacatcact gagcttagct
     5521 ccaaggtggg atctgctgca caccttgagt atcactcaaa gattattgca caaaagtata
     5581 tccagcggga acttattcgc gttgccaccg atattgaacg acgttcattt gaggatgata
     5641 ttgatgttga tgagctgctg gactactccg aaatggaact ctttaaggtg gcagagggta
     5701 atattaaaag cgatactcaa cccattagca acattgtagt taaagcattc aagcagatag
     5761 aggaggctgg taagcgcgag gatggtctta gcggagttcc atctggttat actgacctgg
     5821 ataggttaac ctcgggttgg caaaaatcgg acttaattat tgtggcagcg cgcccttcaa
     5881 tgggtaaaac agcattcgta ctatccatgg cgcgcaatat ggccgtggag tacgacgttc
     5941 ctgtggcatt cttctccctt gaaatgtcaa acattcagct ggtaaaccgt ttgatcattg
     6001 ctgaatcggg catcccctcg gaaaaaatac gcaacggaag gctatcgaat gacgaatgga
     6061 ctcagctaac cgttaaaacc aagcagctag ccgatgccaa gatttttatt gatgataccc
     6121 ccgcgctctc catatttgag ctaagggcta agtgtaggag gcttaaggcg cagcacaaca
     6181 taggaatagt gattattgac tacctacagc tgatgaccgg cccaaccgat accaagggta
     6241 accgagagca ggaggttagc accatttccc ggtcgctaaa ggcaattgca aaggaactcg
     6301 atattcctat cattgccctt tcgcagctaa atcgttcagt tgaaacccgc gggggtaata
     6361 agcgtccgca gctctcagac ctcagggagt ctggtgccat tgagcaggat gccgacgtgg
     6421 ttatttttat tcaccgccct gagtactatg gctttgatgt tgatgacagc ggcaattcaa
     6481 cccttggcat tgctgaaata attgttgcca agcaccgtaa cggtgcagtt gatgatgttc
     6541 gactgcgatt ccagaaggaa atggctaaat tctccgattt ggacgatatg actattattc
     6601 ccaatactat tactcctacc tatacatcct ctgcccccgt tacctttagc tcaaagatga
     6661 acaacgaccc tgttgacgat tttacgttta atcccggtaa gggaattgat aataccaata
     6721 taccttttta gggttaaagg atttttttaa tctcaactag ctccctcacc tcaaaggtaa
     6781 ctttggcctg gtgagggttg cttttggggt tgaaataaat ttgatccata ccgaacttct
     6841 ttgctcccag tatatctatc tcaaggtcgt cgcctatcat aatactctcg cccttacggg
     6901 cgttagcctt tgataatgca tacccaaagg ctcgcttatc gggcttgtgg aatcctgagt
     6961 gttccgatgt tacaacccac tcaaagtact tatcaatgtt gcttctttgc agtttaggta
     7021 gctgaacctc tttaaacccg tttgagataa cgtgtagctt atacctattg gccaggtaat
     7081 cgagagtttc acgagtgcca gggattagcg cagtttttaa gggcataatg gttaagtact
     7141 catcgccaat ggtttcggca aggggtttgc tttcaatacc atactcagcc aaggtaatct
     7201 caaaccgctt gtagcgcaga acttccttag tcaactcacc tttaaggtac ttatcccaca
     7261 ggtaatggtt gtttcggtta aagcagtcaa tgaaagtatc aggattagga aaagccaagt
     7321 ccagcttatg gttagcgtag atttcagcaa gcgcatcgcg catgttctgc tcaaagtccc
     7381 atagcgttct gtctaaatca aaaaaaatat gcttgtaagc gttgtgcttg aagaaaaagt
     7441 tgagtatcat ggtgtagtat taacggcaca aaaatagtta tagtaattaa tgcctgcaaa
     7501 cctataactt cagaatagga gtcaagcaag gttaccctta cttttttact tcattgagtt
     7561 tgatttcgtc ttatttaaat gataaatgtt aacttgggaa aaagtctgaa tgaattattc
     7621 atgttttgtt cagaaagcct atgcctaagc attaaaactt aaatgtatag atttctacaa
     7681 aaaaccttaa cttgtaaatg gaaatgggca atattaaaat ctgttatttt attaacagtt
     7741 aatttttgcc tttgtatctt tgagctgaaa ttttaaaccg tactagaatg agcacatcaa
     7801 atccaaccat aaaagtgctg atggtagatg cagcaagtgg cttttacaag gtgaaccgtt
     7861 tccaagtggg cgattttttt gggccgattg atttgggaat acatctatcg cataagcata
     7921 acagcttaaa cataggggtt ggacttttag ctggttcaat ttttcctggt tctaaccgcc
     7981 tgattgtaaa cggaatatca ccgtgttggc atggttttta catatcgtcg atggggggtg
     8041 caggtttggt attcgataac cttggaataa atatgttttc cattgtaggt aaggctgcag
     8101 caccgtcaat cctttatctt aaccgtattc atggtgagga aattgaggta gaacttctcc
     8161 ctgtccggcc acgaaccatt tgggccgagg gtcgtggtgg ggtttactcc atgatgcagt
     8221 atgcactcaa ccagtttggt agccgttatg agaatgaacc ccgaatactt gctaccgggc
     8281 cagcatcgat gtacaccgat tttggcgcta ttgcctcagc tccaatccgt aagaatgaga
     8341 ttacctatgt tgacacctgg gctggccgtg gaggctttgg taccaaaatg gtgcagcagc
     8401 acaacattgt tgctattatt tacggtggaa catatatcga tgaggacttc cgcgaccgta
     8461 ccgttgccga tagctggttt gaggatcgct accagaaacg ccttgctgcc aaggatatgg
     8521 aggctaccac caagtatcgc ttcgatccta agtttgaaac cggtggaacc tttggcgtaa
     8581 actacgccac catgaagggc gatattatgg ccttcaacta tcgaacaatc tacatgacgg
     8641 aggaggaacg ccttaaaatc cataacgatt tcatactaaa ccactacctt aagcagttta
     8701 acgaggaaac cattaaaacc aagcagcaac gtacctgtgg tgaaccttgt ggggctgtat
     8761 gtaaaaaaat gcacggggag ttcaaaaaag actatgagcc ctaccaaacc atggggcctc
     8821 tttgcggaat attcgatcag cgagctgctg agaagttaaa ccaccatgcc gatatgcttg
     8881 gtttcgatgc catttcggtg ggtggtgtgt tatcgtggct tatggactgc atggccgatg
     8941 gtcttgtaga gcctcaagaa cttggcgtgg ccgatattcc cgtatttaca cccaatggtt
     9001 tccgtgttgt ggaggactca atgcataacg ctaacattgg aattgcttta ctcgaccaga
     9061 tggtgaatcc caatggagtt tttcacttgc gcgagggggc acgtaagttt gcccgtcatc
     9121 ttgcccggcg taagggaaca tctgtactcg ataagtttat atacataggt tttgcgcgct
     9181 ccggctggat ggtacccaac cagtactgga cacccggagt actatcgccc atggcaatta
     9241 tgggtaagta ctacatgtac tacggaaagg attttgttcc tcctcgtcag ttgggaaggg
     9301 aaaacgctaa gcgcatgcta aacgagcttg taatggataa cctgggaatg tgtcgcttcc
     9361 acagggcatg ggctgaggat attctccctg atgtaattga gcaactttac gggttaaagg
     9421 aaaagtttca acgatcgata cttcttactg caagccgaat aaacagccgt aacgcatcca
     9481 tattctggga aagcgaaagg aatatcgatt ttgtttactc ctttctaagg aaaaagaagg
     9541 aggttgatgg tgcaagcaat cctgaactcg acagatggat aaagtatttt gaggaggaca
     9601 agcgaagtgc tgcacttgat ttctggtacg aaatccataa gggcgtacat gaatcgcttc
     9661 ttgaatttca ttaatagtta tatttgacga aaaaaatgaa gatcattcgt aggatacttt
     9721 ttttcattgc gctattacta gttgctatag ctgcattcct ttactggggt acttatgagg
     9781 aaggcgtaat ggcagggaaa ataattcgta ttagccagaa aggtttgata ttcaaaacct
     9841 atgaggccaa gttaggcctt gagtcgtttg gcgcattaag gggaaccagc cccattgctg
     9901 aaaccttcga tttttcggtt gaacccagta atcatgaact tgtaaaggag ctagagcagg
     9961 tagctctaag tggtgaaagg gtaaacatac attacaagaa acgctatatc agggttccct
    10021 ggcggggcag caccaagtac tttgcaacaa gagtggaacg tttacagagg taaaataaaa
    10081 aaggcagcca aatggctgcc tttctacttg gtatacttga aagaatccta gtaatccctt
    10141 ctgccaccgc gaccgccacc acggctatcg cgaccctcgc ggctgtaacc gccacgacgt
    10201 tcaccgtaac caccgccacg acgctcacgg ttgaatccac caccgttgcg gggcttgtcg
    10261 gttttgggtt ttgcttctga aacgctgatt accttaccat cgtattcagc gccggtaagt
    10321 tcctcaattg cagtacgggc ctcttcatca ttggccatct caacaaagcc gaatcctctc
    10381 gaacgccctg tttccctgtc ggtaattact ttggccgagt taatctggcc atactcttcg
    10441 aaaagttgcc ttaggtcgtc atcgttaatg cggtaactca gacctgacac gtaaatgttc
    10501 ataaactaaa attagattgt gattgataaa taaattgttt ttttgattga ggccttgctt
    10561 aactgatgtt ttgtcgggaa tcggctagat gcggcaaaat attctgtgtt gaagcgtttc
    10621 tcaattcgct gcaaaggtag tgcaattaat ttaacaatca ccattttcgg aaaattattt
    10681 ttacaaggga aaggcaagga agcttaagtc agatattagg aacccaaaag tgtattcttg
    10741 aaactcaatc caaatcaaaa ccatgtgcac taacattttt aaaaaaaagt gggtacctga
    10801 atgggcaccc actgattaag agaagtataa ttgattttta cttggttgct tcttccaacc
    10861 tacgcataat ggagaagatg aaaattgccg agagtaagca gcaaacaata aagattgtcc
    10921 agagctgcca tagctcaatt ctttcccaga agaagctgcc tacaaaaagc aacttgtttc
    10981 caatggcagt agcaagtagc caaccaccct gcattaatcc ctggaagcgt tgaggagcaa
    11041 cctttgatac aaacgacaat cccattgggc tgaggaataa ctcagcaatg gttagaatca
    11101 ggtagctgct aattagccag taaggcgaaa ccctgcccca ttcggttagc ccttgcgcct
    11161 caatggtttt gggagctatt aggtgaattg aactcaacag cacaaccaca aatccaattg
    11221 cagcaattat catccctata cctattttct taggggttga gggctcaata ttccgtttgt
    11281 tcatccaggc aaaagcaccc ataacaggga atgtaagcac tactatgaac aatgggttaa
    11341 acgattggaa aacctcgggg gcaattgggt tggaagcccc aaatttgata gcctggtagt
    11401 aggttaggta accaaggcca agaaacatta ccagtccaat cattctctca cgaatctgtt
    11461 tacgggcaaa cattaacacc aacccagcaa tggtgccaat aaatgaaagc attgaccata
    11521 tgttgaagaa gatattggtg aaaggtccaa cctccttaac ggtatagtcg cgggcaaata
    11581 gggtcaaggt taaaccgttc tggtggaacg acatccagaa aaagattaca accacaaata
    11641 cgagtagaag cgaggtaacc tttggaagtt cttccttggt tgacatcatg aacatcatgg
    11701 ccacaaagcc aaagaataga ccaatggcca atcctaaagc aatttcggct ccgagtaacc
    11761 agaaaatcgc tccagtcaat aatacaaggg caatgggtac aattacgttt agaaggttct
    11821 ttttggtaaa tagcgatccc gcagctggtg tagtgctaac ttttttctcc ttattgggta
    11881 attttttgtt gaaaattaca tatacaagta gcgagatcac cattgcaact gcagcaacac
    11941 caaaagccca gttatacccg cgcgaaaagg catcaatgta tttatgagca aaatcggtga
    12001 gctgttcagt accggtaata gtggcacccg aaaccttgct ggcaaggctt tcgaactttg
    12061 cgagcgactc aggatcggtt accttaccgt ttaggaatgc atggcaaagc gagggtaggc
    12121 tggcatcgtg ctcaaagcct tggctgcgaa ggaaccagtt acggataccg gttgcaacaa
    12181 atggggcaaa gaatgcacct acatttatac ccatataaaa aatcatgaaa ccagtatcgc
    12241 gaacctttga gtattcaggg ttgtcgtaca tctggccaac tactgcctgc aggttacctt
    12301 tgaataagcc attcccaaat gcaatgatga aaagcccaag taatgtcatg gttaggctca
    12361 tgccaggcat ggcaatcatt gcgtagcccc caaacataat gattatcccc agcagaatta
    12421 ctgttttata cctattggtt gcatcggcta ttaatccacc aataagggct aacgcataaa
    12481 tggcaaagta aaaccagctg tagtaaccac cggcttcctc ggccgatagg ccatacttgg
    12541 cttggagaaa taacaccagg atggccatca tggtgtagaa accgaaacgc tctcccatgt
    12601 tggagaagaa cgctaccagt agtcctttag gatgattttt caacatataa attatggttt
    12661 tggtttatct tcaatttttc aagattgaca aaaatagtat ttaatggttt atttccaaat
    12721 ccacaaatta tgttgtataa tgtttttgtt aagcttattt tacctaaatg ttaaaagcga
    12781 taaccattta ttggttttaa tttgtaaata caattaaccg aagtgccatg atttaacaga
    12841 ttcagacggg attagaggaa tgagtaggaa tgagcggaat atgatggagt tagaggaagt
    12901 tagagggggt tagaactagt gaaaagtgaa aagtgaaaag ttaaaagaag aattgaggat
    12961 atgagggggt tggagagctt tatgttcgtg aaccgtgaac cgtgagccgt gaaccggaag
    13021 aattaagaat atgacggaga tatagggagt tatagggaag ttgaactgtt aaatctaaat
    13081 accatggatt aacataatca accgggttaa aaaaagggag agggatacat ttatagagga
    13141 gattcatcaa tggatttttt acaattgacc tgttcaggat aaacaatcgg gaaaaattag
    13201 taacttagca ctatagcctg aatcaatgaa agtattcaaa acaagccaga tcaaaagcat
    13261 cgatgcttat accatagaaa acgaacccat tgagtcaatt gatttgatgg aacgggctgc
    13321 aacagccatt ttcaactggt ttcgaagcaa ataccataag aatcgaagga ttaaaatctt
    13381 ttgtggcccc ggtaataacg ggggcgatgg gcttgcattg gctcgtttgc taattaatac
    13441 gggctttaac gcgcactgtt actacttaaa agccgattct tactcagccg actttttaac
    13501 aaatcttgat cgtctaaagg agttaacgcc ggaattattt ttagttaaac atcaatccga
    13561 ttttcctgaa attcataccg atgatctggt agttgatgcc ctatacggtt cggggcttag
    13621 tcgatccatt gaaggggttg ctgctcaact aattgagcat atcaactcat cgggggctaa
    13681 ggttatcgct attgatattc cctcgggtct ttttggcgag gccaatccag tacccaacaa
    13741 taaccctgtg gtaaagtccg actactgtct tagcttacag tttcccaagc tggctttttt
    13801 tatgccagaa aatgagcaat ttttaaagcg ctgggtagtt ctacccattg gtctgcatac
    13861 ggaggctatt gaaaatacgg ctacaccgta ccattatacc acggcaagta gtatcaaaac
    13921 cgattttttc cagttgtcaa cctttgccca taaagggact tacgggcatg ctctagttat
    13981 tgcaggttcc aacgggatga tgggagcggc ttgcctatgt gctaaatcgg ttgtgaggag
    14041 tggggtagga ttagtaacaa tgcatattcc caaggtaggc tataccattg cccaggtaaa
    14101 tgttcctgaa gccttaacta gcatcgatag ctgtgaaact cacattaccc aattccctga
    14161 gttggatcga tatcaagcgg tttgcgtggg cccaggcatt gcacaaagta ccgaaaccga
    14221 tcaaatgctc gtcaagttga ttaaaacctg ccaattacct ttggtggtag atgccgatgg
    14281 actaaactta ctttcaagga atattgatgt actcaatagt gttaacaatg cattgatttt
    14341 aacaccacat cccggagagt tcgatcgact ttttgggaaa tcgcagtgta gctgggagag
    14401 gcttgaacgt gcaagagaaa tggcacaaaa atacggggtg ataattgttc taaaaggagc
    14461 ctacacaagg gtagtattgc ccagtggcaa tgttcatttc aactccacag gaaatccggg
    14521 gatggcaacc ggaggaagcg gcgatgttct tagtggaata attaccggat tgcttgctcg
    14581 tgggcttccc ccggccgatg ctgccattac cggtgtttat atccatggcc ttgcaggcga
    14641 cttggccgcc gacgaatttg gacaaattag cttgtgtgct tcggacattg tgaactatat
    14701 ttgtaaagca ttccaacaca taaccaacaa tgatgagtaa aaatcttttc ttagcagttc
    14761 tatcccttgt attattgggc tgtaatagta aaaccattca agttgaaccg gctggtaaaa
    14821 caattgacta taaaaaagtt gcagtttact attcgttacc ccgaacagtg gtttacgtaa
    14881 aggttgttag ctctcaaagc gaatatttcc ccggcccata tgccgattat gccaaaaagt
    14941 ttttgggtat cgacggggta agtaataccg aaaaggttga gtatctaatt gagaaaatag
    15001 atgtagaaac caccgctgaa ccagatccca aagcagtctt tgctatttac ccatcgggaa
    15061 agggttatgg taactttata aatctttcta atgaagggtt aatattacct gttaaccaaa
    15121 cccctgaaag tagagccatt gctaaaaaca aagaagtaag cagtaaagga gaggtaattt
    15181 ataaggattt atcggttaac ccttttgttg ctgaggaaac aaccacattt tacggtagaa
    15241 ccctgcgcga cagcgtttac gttagggtac ccatacagcg ttcaatgacc attgaacgca
    15301 accttgagga aaaagctaag gaggcagccg attttatttt tagcatccga aaaaaaaggc
    15361 tcgattttct tacccctgat atcgaccatc cattcagtgg cgatgcactt aagttgattt
    15421 tgaaggaact tcaaaggctg gaggacgaat atctggcact atttgtaggt aaaaggttta
    15481 ctcacacatt cactcaaacc tatagcttca ttccttcgaa caccgaaggc gagagcgcta
    15541 tccttttccg tttttccgaa agcaaaggat tgctttcatc aaccgacctt tccggacgcc
    15601 caatactcat tgagtttgat caacctgaat atcctgagaa tattgatgct actaagaata
    15661 gcctggaaac cattgcttta aaagccaaaa tcgacaggtt ttactacagg atacccgttg
    15721 cagttaacat gagagttatt gatggcaagc agctacttgt tgaaaagcgt ttaagcatat
    15781 tccagtatgg actgctagcc caactgcctg ttaattttcc aggtaagtga ttaaggattt
    15841 gctactgtat ttagtttact tacaaacttt gcccgaaaat cttcccattt ctgggttttg
    15901 taagcatcgc tttcaatgta agccattatt ggttctagct gttcaggagt aaggtatccg
    15961 gcaatgtagg taattaggtt attgttctcg tccataaaca ctattgaggg atacgataac
    16021 cttccttgta gaatagctat agcaaactca tgcggattgc gttgcccctg cccctgcccc
    16081 ttattcttgt ataccttacc tttatatgtt atatcttttg aaccttcggc attgaacttt
    16141 acggcatgaa acttttgggt gaggtacgag gctattacag ggttggtaaa agtattctca
    16201 tccatcttct tacaccagcc acaccagtcg gtataaacat cgatgaatat ttttcggggc
    16261 gttttttcgt ttacctcaaa agccctatct atttcaatcc agttgatttt tgtttcttgt
    16321 gctatggaaa ccgaagcgga tatgccaagc atccaaatag ttaaaaccca gattctgttt
    16381 ttcatggttt tgattttggt taacggtaaa tgagttcaaa aataatgcct taatatcact
    16441 ttgcatcacc atctttatac cagcctgagt aaaagaggta gttgcgggca atacgctcat
    16501 tggtttcgcg gctaagttca ggggcaacct ttttgacaaa acgggctgga actcctgcat
    16561 aaacacatgc gggttcaaca acagtgttct ccaataccag tgcgttggca gcaactattg
    16621 ctccttcgcc aactttagcg ttgtccataa cagtacaacc cattcctagc aaggctcccc
    16681 ggcttacctg ggcgccatga agtattacac catgtccaat cgatacatca tcgccaacct
    16741 cgatggttgt tttctggtag gtagtgtgaa gcaccgcatt atcctggata ttaaccctgt
    16801 tacctattct aatggagtta acatcgccac gaagcacagc gccgtaccaa atactgcaat
    16861 catcgccaat ttcaacatca cctattatca ctgcagtctc agcaataaag cagttcttac
    16921 caattttagg tgtaattccc ctcaagggtt tgattattgc cataccattt tgtaatttac
    16981 tgcaaattta ctacttacta acagttatcg tgccggttga gttcaaaaag tttgtgaaca
    17041 ttagagttta tggcgatctg aacgattttt tgccacaagg caaaaagcat aaggatcagc
    17101 gtgtaggttt ttttgggatg cccactgtaa aggacttggt tgaatcgata ggggttccgc
    17161 atgttgaggt ttttctgatt ttagccgata gcctaccggt taaatttgat taccgtcctg
    17221 ctaacggcac actaatttcg tgttacccaa aatttacttc attgccatta tcggcaattg
    17281 ctttaagacc tgattttgaa aagcccccca ggtttattct tgatgttcat ttaggtcgac
    17341 tggccgcata cctccgcttt tgcggaatcg acacactata cgataagtcg tttaatgaca
    17401 atcagatact tgatattgca caagccgatg gccgaattat tctgacccgc gataagggta
    17461 tcctgcgtaa cggccaagcc caatttggat attttctcag aaacaccaat cctcagctgc
    17521 agcttgccga ggtggttcat tactttgacc ttgctcacta ccttgatccg ttttcaaggt
    17581 gctcactgtg caatggtgag gtagaacagg ttgataaatc catggttaaa aacttagttc
    17641 cttcaactac ctaccagatt tacagtgagt tttttcagtg ttccagatgt gggcaggttt
    17701 actgggaagg acctcacttt atcaggattt ccaagctgct tagtaggtat tcaaagtaat
    17761 tattagctta tttttatggt taaggtaaaa atgaaaaaca tgccgtaaaa tatgttttaa
    17821 aacgattgct gtgtataaaa aagcctaatt ttgtgtcata aataaataag tagtaatcaa
    17881 tataacattc tggatatcag atttatataa ttcaggatgt ttaaaaacta aacagcatct
    17941 ttatggataa acaaaaaagt gtccaagagc tggacgaggt tgttgttcga ttttcaggtg
    18001 attccggcga tggtatgcag cttaccggaa ccctgtttgc cgatgcatcg gcactttttg
    18061 gtaacgatgt aagcactttc cccgattacc catcggaaat acgtgcccca cagggcacca
    18121 ttggtggcgt atcgggtttt caggtgcact ttggaaaccg aaaagttaat acccctggcg
    18181 atttttgcga tgtgttaatt gcaatgaacc ctgcagccct aaaagccaac gccaaatggg
    18241 taaaacgtgg cggtatgatt atagttgatg ccgaccagtt taatgaaaag gggtaccagc
    18301 gtgccggcta caaaactttc gatgcagtat cggagttaaa cctgcaggat tacaaggtga
    18361 tttttgctcc cattacaaac cttaccaagg aaagccttaa ggatagcgga ttggacaata
    18421 agagcatcat ccgtagtaag aacatgtttg ccttgggtat ggtttactac atattcgata
    18481 ggccattaaa ctttaccgag aaattctttg aaaagaaatt ttccaaaaag cccgaagtag
    18541 ttgaagcaaa caagaaagtt ttacgcgatg ggtttaacta cgccgcaaat attcaggcca
    18601 ttgctagcac ctataaagta aagcaggcaa ctactttacc caagggtaaa taccgtaaca
    18661 ttaatggaaa cacggctacc gcatggggtt taatagcagc tgccgaaaaa gcgggcttac
    18721 ccctgttttg tggttcatac cccattactc ccgccactga aattttaata gagctagcca
    18781 aacgtaagga tttaggcgtt aaaacccttc aagctgagga tgaaattgct ggaatatgta
    18841 ctgcaattgg cgctgcatat gccgggaact ttgcagtcac cactacatcg ggccctgggc
    18901 tttcactgaa atctgaagca atagggcttg ccgtaatgac ggaacttcct ttagttatag
    18961 ttgatgttca acgcggtggc ccttcaaccg gtttgccaac caaaaccgag caatccgacc
    19021 ttatgcaagt tctctgggga cgtaatggcg aggcgccagt ggtagtaatt gccgccagta
    19081 ccccaagcaa ctgcttcgat tacgctttta tggcaggtaa aattgccatg gagcatatga
    19141 ctcctgtagt acttttaacc gatggttata ttgccaacgg ctctgagcca tggcgcattc
    19201 caagcatgaa ggattaccct gaaataaaac cacctattgt tccagaggga accgaagggt
    19261 atatgcccta tgctcgcgat gaaaaacgct tagcgcgtgg ttgggctttc cccggtaaaa
    19321 aaggtattgc ccatagagtg gggggcttgg aaaaggattt cctaaaaggt accgtttcac
    19381 acgacccaac caaccaccaa cgaatggtcg atatccgtgc tgcaaaggtt cagcgtgttc
    19441 aggagttcat ccctgagcag caagtgattg gcgaccccga aggcgacctg cttgttattg
    19501 gttggggcgg aacattcggt catctgttcg atgcagttca ggaaatgcgt gcaaacggaa
    19561 aaaaagtttc actgtgccat ttcaactaca taaaccccat gcccaaaaat gttgccgaca
    19621 tttttggtcg tttcaaaaag cttgtggtat gcgagttaaa catggggcag tttgctaacc
    19681 acttacgaat gaattatcag caattcaact acctacagta taacaaggtt cagggtctac
    19741 cattcaccgt ggtggagctg gttgaaaagt ttaaccaaat tttggagggt aaatagccat
    19801 ggaagaacaa tatatgaatt acaccccaca ggattttaag agcgatcagg aagtgcgttg
    19861 gtgtccgggt tgtggcgacc acgccattct gaatgccgta caaaaagcct tacccgaggt
    19921 atcaaaacag ttaaagtata accttgaacg ctacgtattt gtctctggta ttggttgttc
    19981 cagccgtttc ccctactacg ttaatacctt tggtttccat ggcatacatg gtcgtgcatc
    20041 ggccatagca accggagtaa aggtggcaaa cccaacgctg agcgtttggc aggttactgg
    20101 cgatggcgat gcccttgcca ttggaggtaa ccactttatc cacgcagtaa ggcgtaacat
    20161 cgatattaac atcttacttt tcaacaacca gatttacggg ttaaccaaag gtcagtactc
    20221 accaacctcg ccacttggag caattaccaa aacttcacca tttggtacgg ttgagcatcc
    20281 ttgccgccca ggtgaactgg ttattggcgc tcagggaaag ttttttgccc gttcgctcga
    20341 tgttgatgtt aagcttaccg ctgagattat ggttgaagct gctaagcacg atggcacgtc
    20401 ggttgttgaa attcttcaaa actgcgtcat ttttaacgat ggggcttacg attacataac
    20461 cgaccgtgaa catcgcgacg atagagtgat tgttctccgt catggcgaaa aaatggtatt
    20521 cggaaaaaac agggataaag gactaattct cagcggactg agccttaagg ttgtaaccat
    20581 tggccagggt ggctttacca tggacgatat acttgtgcac gatgcctata acccaaatcc
    20641 tggtgttcac atgatgcttg ccaacatgga gtaccccgat taccctgtag cccttggtgt
    20701 aatccgtaac gtaaaagact ctacctacga tgataacgta cgcgaccaag tgcttgaagt
    20761 gatgaaaacc gccaagatta agtcagttga cgatttactc aacagtggcg atacctggga
    20821 agtataaaac cttattatct ctttttaata aattaaggct gccaacttca ggcagccttt
    20881 tttcaatatt ttttttcact attacgctga acattagttt atttagttaa taaatgctaa
    20941 atttgcttat ttgatgattt aaaagtttaa tgcaattggt tgcaaagtat ggtaaagcaa
    21001 aagtttatta aggcattaat cgcactgcaa tttattctta gtttctcagt tagcgaggtt
    21061 aaggctcagg atagtttcaa gcaaatcttt agcgatgcag agtattactt cttccttcag
    21121 gacttcaagg aggcacttcc cctttaccag agcctatacc aacaagattc taccaatgcc
    21181 aatatccttt acaagctagg tatgtgttac ctgaacatac ccggtttgaa acagaatgct
    21241 ataccttacc tagaaaaagc atcaaagaat gtaaacccta aataccgtga agggtattac
    21301 cgcgagactg ccgcgcccat tcaaacctat ttttacctgg gacaagccta catggtaaat
    21361 tttaagtttg atgatgccct gctggctttt cagaagttca aggataatat tgatgttaaa
    21421 gatgtttaca acctcgacta tgttaaccag cagataaaag catgcgaggt ggctcgcaat
    21481 ttcatttcca ggccaattgt gatgaaaacc gaacatatcg acctgtttcc cgataggaat
    21541 aagaattgca attacccagt aatatcaggc gataggcaaa ccatggtgtt tacggttaag
    21601 gaaaaattct atactgcagt atactattcc cggtggattg atggcaagtg gagttcacca
    21661 aggaatatta cacttgacct tagggttgag ggcgaacttt atactaccgc ccttaactac
    21721 accggcgatt acttgattct ttttgtcaat gaagtaacct ccggcaacct atactactca
    21781 acgctggtag gcgataagtg gcaacccgtt aaaaagttac cagcaccaat caattcaaag
    21841 gattgggaga cctttgcgtc gctatcggtc gatggcaaac agatgtattt tgtttcgaac
    21901 cggaaaggtg gctatggcgg aaccgatatt tacatgtcca gtttgcagcc cgatggcaaa
    21961 tggagcaacc caataaacct tggtccacag gtcaataccc catacaacga ggagtcgcca
    22021 attgtatgcc ccgacggacg aacgctttac tttgcctcgc aggggcataa cagcatggga
    22081 ggattcgata ttttttacag ccgaaagatt gatggtaatt cctggtctat gcccataaat
    22141 cttgggtatc catttaatac acctgacgat gagttttact tttaccccct cgattcactt
    22201 tctggcgtaa tgccgatggc catttccaat caatcaacct tttacgaact ttacaaggta
    22261 aacatctacc ccagtatatc acgtaagata gaactctttg gcaaggttaa tctctctgac
    22321 aatgccgata ttaaatccga cagtatcgct attttagtta aagatactgc aaacaacctt
    22381 attgccatgg ctttaccctt aagcgatgga acttacagcg ttgcaattaa gcctggcaga
    22441 tactcccttg aggctaccag ccaattttac gtaatgaacc cactgcagct tcacataccc
    22501 accacctata accaggaaaa gtatctatta gatatcaacc ttgatgctaa gccaataacc
    22561 aaagaggagg tgattcggtt caactatgta cttttcgatt ttgacagcta cgagctgaaa
    22621 cgcgatgcac agtttgaact tgaaaaggtt tacaagctca tgactgatta tcccgacttg
    22681 tatatcgagg tgattggcca tactgacagc aagggctcac ccatgtacaa tctcatgctt
    22741 tcggcccgta gggcaaatgc cgtagctgag tacctggtta ataagggaat tgacgaaaaa
    22801 cgatttgtag taaggggtat gggctcactg gtaagctttg ccgctaacac caaccccgac
    22861 ggttccgata accccaacgg ccgtaagcta aaccgacgag ccagcattag ggtatttaac
    22921 ccaaacaaga acctaaaaat tgagtttgtt gatgttcccg aacacctcaa acctcaaacc
    22981 cagaactata ccataatgct tgcacctatc gacgatacaa tttctcctga tctaattaaa
    23041 gccattgaga agaagtttaa tatcaacctt cgtgagtttg taattggatc acgacgattg
    23101 gtttgtatga atgtttacaa gtcaaaggcc gacgccattg agcatctcaa taccattatt
    23161 gatatgggtg catcaagggc cgtgctggta aatgaggttg aacttcaacg ccttgtggct
    23221 gctttgcaga aaaatcttat aacaaaacaa agcgttttta ccattcttgt tgccacaagc
    23281 gagataccct taacgctcga ctttttccgg gggctatatg taaccgagga ggttggtaac
    23341 gacgggttat accgttacta ctttggagcc ttcaatgaga aagctaaggc taccgagatg
    23401 ctggaaaagg taaactcaat gggattcccc aatgcaattc tggtaaaact tgagaaaagg
    23461 taatggaggg taatgtttta gaaaacagcg ttgtaaagct tcgtgcacct gaaccagagg
    23521 atgtcgattt gctttacagc tgggaaaaca acatggaaat atggaaggtc agtaatacgc
    23581 ttacgccttt ctcaaaatac ctgctcaaaa agtatataga gacatcgcat ttagacatat
    23641 gggaatccaa gcagctacgg ctgattattg aggctaagga tcagaactcg cttatgaatg
    23701 ttccggttgg ccttgttgat ttgttcgatt ttgatcccta ccatttgcgg gcgggcgttg
    23761 gtgttttgat agccaaaaac gaagaccgca gaaaaggtta tgctactgag gctttaaacg
    23821 tgctaatccg atatgtattt gaggtactac agctaaacca gctctatgca aatgtgtcgg
    23881 caagtaatgt tattagcttg cgcttgtttg agaaccttgg ttttactaag gttggtgtaa
    23941 aaaaggattg gttaaaaaca ttgaatggat gggaggacga ggttttactt cagctattaa
    24001 atcctaaacc tcgcctaccc aaaagttaaa actggcgata attttagcct tactggttaa
    24061 aattgaaagt gctcctcaac atttttccaa agcattgctg cttcagggtt cttgttgcag
    24121 aactcattgg tatgcttgca gtacgagtat tcgttttgcc attccttacc gtatgcaaca
    24181 atttccgcta atgcgttggc aatgtactct acttcggcat tggttgttgt tggatgtaac
    24241 gaccatcgaa cccatccggg tttttcggac aagtcgcctt tattgattaa atcagtaatc
    24301 cgatgcgatt gctccttgct tacattaagt aggaaatgtc cgtatgtgcc tgcgcacacg
    24361 caccctccac gcatttgtat gccatagcgc tcgctaagta gggttacaat aaggttgtag
    24421 tgcaagttat caatatagaa cgaaattgct ccaattcgat ttaggcagtt atcggctaaa
    24481 atgtgtaacc ctttaatttt actcaaccgt tcaagggcaa ggtgaactag ctcctcttcg
    24541 cgtttttcga tattctcaac acccatttgc tccttcaaac gaatggctaa tgcgctacgg
    24601 attgcttgca aaaagcccgg agtacctcca tcctcgcgtc gttcaatatc atcaatatat
    24661 ttatactcac cccatgggtt tgtccagtca accgtgccac ctccgggttg atcgggcaca
    24721 cggtttttgt aaaggtttga gttgaagatg agaacgcctg aggcaccagg gccacccaga
    24781 aacttatggg gcgaaaagaa aatggcatct aaacgctcca tgggatcggc aggatgcata
    24841 tcaattttta cgtatggtgc cgatgctgca aaatctacaa agcataaacc cccagcccca
    24901 tgcatgattt tagcaagctc atggtagggg gtttttattc cggttacatt gctgcatgcg
    24961 ctaaatgaac caattttcat tggtctatca ttgtatcgct ccagtgtttc gcggagtttc
    25021 tggggcgata ccaaaagatc cttatcgggt tcaaccacta caacctcagc aatggtttcg
    25081 taccacgatg tttggttgga gtgatgctcc atgtgtgtaa caaaaaccac gggcctttcc
    25141 ttggggtttt tgaatacaaa gcttttgttc ttaaggccaa gtattctttg aaatttgtta
    25201 attgacgatg tcataccaaa ccctgatgta acaattacat cctcggggcc agcgttaaca
    25261 tgtcgtttaa tctctttcag ggcatagtga taagcctctg tcatgaactt tccggtaaag
    25321 ctactctcgg tatgagtatt ggcaaccatt ggcccaaaaa gatggagcat tttttcctct
    25381 ataggcctgt atagcctgcc actggcaatc cagtcggcat atattagttt ctgttcacca
    25441 taaatggttt tataggtagc atcgatacct acaatgtttt tacggaattg ctcaaaatac
    25501 ttttcaaggt tactcatatc ttagttatat tttattcctt agctcaggga agccatctag
    25561 ggttatccat ccttttccct cagaaagcca caaaattaaa tgctttaacc cgttagttac
    25621 caacaaatat tcggcattca gcgcatagtt atacctgcat atctgatcaa caacctcgtt
    25681 ggtaatttcc acgttaggcg ctttacattc cgcaagtagt attggctcgc cttttcggtt
    25741 atagactaca acatcggctc tatggtttac gccattgaat gagaaactta cctcaacacc
    25801 tatcaatcct actggtaagt ttaaatcggt tatcaggtag ctaagtaaat gctgacgaac
    25861 ccattcttca ggggtaagtg caataaattt ttttcggatt acatcaaaaa tgtaacggtt
    25921 accattttcc tgttttaccc ggaactggta ggcaggaaag ttaagcgaac ctccgttagc
    25981 tgtgctcatg cagcaaaatt aaaaattttt aaatcatcct aggtcttgct aacgttttcg
    26041 ccagccaaaa caagctggta attattagcc tttactagaa aatttcacca aaatagcaca
    26101 tcacttttgt taagtatgtt taaaaacact attttaagat tgagcgaata gccgtatctt
    26161 tgccgaaata tacttgttga taaaatataa atgtgttggt atgaagacaa aagaagaaat
    26221 tgttgagaac tggttaccaa ggtataccgg gcaagcactt gaggattttg gttcccatat
    26281 tcttttaacc aactttaggg ggtaccttga attctttgct aattacttta atgtccccat
    26341 tgttaatcca aatgccaaca tgccctgtgc cactgcaaat ggtataacca tgatcgattt
    26401 tggcatgggt agtcccaatg ctgcaactat aatggatttg ttaagcgccg taaaacccaa
    26461 agcggtactt ttccttggaa aatgtggagg cctgaaaaag aagaacaacg taggcgattt
    26521 tatactaccc attgcagcta taaggcacga aggaacctca aacgactata tgccccctga
    26581 ggttcccgct ttgccagcat ttacgctaca gcgtgtagtt tcctcaacca ttcgtaacca
    26641 caaccgtgat tactggacag gtactgtagt tacaacaaac cgaagggttt gggagtacga
    26701 cgataatttc aaggagtacc ttcgccgtac ccgtgcaatg gcaattgata tggaaaccgc
    26761 aaccattttt acggttggtt tctataatga aattcctacc ggggcacttc tgctggttac
    26821 cgaccaaccc atgatttcgg aaggggttaa aaccgctgaa agcgatcaga aggtaaattc
    26881 actctttgtt gaggagcaga taaagattgg tatagaatca ttaacccgtt tgataaacaa
    26941 cagcgaatcg gtaaagcacc tacgttttta agtttgatat atggccaagc agagtcagac
    27001 agttgaaacg tttcaaaata ttcttcagga gctgaagaat aagatatata aacccattta
    27061 ctttctgacc ggcgatgaac catactttat cgacaagatt gctgagtaca ttgaggacaa
    27121 tgtgctcagc agttccgaga aagcattcaa ccagaccgtg gtttatggtc gggatatttc
    27181 tgctgctgat attatcaacg cggcacgtcg ctttccaatg atgtccaact accaggtagt
    27241 aattgttaag gaagcacaga acgtgcgtaa cctatccgat ttggagatct accttaaaac
    27301 tccgttgaac tcaacaatac tggttgtttg ttacaaaata gccagcgatg gcaagaaatc
    27361 gtccgataag ttctcagggg ttgtaaagct tgcaaagcaa aaggggattt actttgaatc
    27421 aaaaaagttt tacgactacc aaattcccga ttggattgtt ggatacctta aggataaagg
    27481 tattaaaatt gacccactgg cagcaaacct actaactgag tttgttggta acgatttgag
    27541 ccgcttggcc catgaacttg ataagctagc aatcatactt cctgctgata gcaaacagat
    27601 acaggccaca gatattgagc gaaacattgg tataagccgc gattttaacc gttttgaact
    27661 aaccaaggcg cttggcgaga tgaactttgc ccgtgccatg cgcattgccg accactttgc
    27721 acggaatccc aatgccaatc cttttgtgtt gaccattact gctatttacc agtatttcat
    27781 caaaatatta aagtatcact ttttagctga caaatcaaaa gcatcagtgg ccaaggagtt
    27841 gggtgtaaac gagttttttg ttgctgagta cgaaagggca gcaaaacgat acagcccatc
    27901 gcgctgtgta agtattatcc actggctcag cgagtacgat atgcggtcaa agggtgttaa
    27961 taacggttca accgaccatg gcgaactgct gcgggaacta atttacctta tagtatttgg
    28021 gaaatgacct atataatcat tgctattacc gctattgttt cctttgctgc atttggaaac
    28081 agcatcctat ttcagcagtt actgcttaac ccatacatag tatttaagcg gaatgagtgg
    28141 tataggttga tttcgcatgc ttttgttcat gccaattttt tacacctatt tgtaaatatg
    28201 atagtgttgc tatcgtttgg caggtatgtg gaatcaatat taaagcaact acaatctatt
    28261 ggtagcattg gtatgcacca ggttcacttt ctcattctct accttggtgc agcaatagtt
    28321 tcatcgttaa ccacgctgag aaagcataag aacgacccat actaccagag cgtaggtgca
    28381 tctggtgctg tctcggctat tgtgtttttc agcattttct ttagtcctct ggaaaagctt
    28441 taccttatgg ctattatacc aattcctgga attgtgtttg ccattgctta ccttttttac
    28501 tcaaactaca tgagccgtaa gggaggcgat aatataaatc acgatgcaca ctttgtaggt
    28561 gcagtattcg gattcattta cccaatcctt attgacccaa agctgttttt tttcttttta
    28621 aagcagttgg ggctatagtt tgatacccat gcaagccgag gcgcaaatca ctatacaccc
    28681 tgatggttta gatgcaagtg ttttgcaact ggtagctatt gttagttaca tgaatgggaa
    28741 ttggctatgg gtgcgtaata aaaaccgtga tacctgggaa ctaccaggag gccatatcga
    28801 accgggcgaa ttgcccgttg aggctgcaaa tagggaactt tttgaggaaa caggagcaat
    28861 tcgatactcc ataaagcagc tatttgatta tacagtgcgg ctcgatgggg ccgtatccta
    28921 tgggcgaatg ttttatgccg aaataactga acttgaaact ttacccaata gtgaaattgg
    28981 ggaagtaaaa gcatttaaga gtatccctga gaatttaacc tacaaaaggg tacaaccccg
    29041 tctttttgaa atggcaattg aatacctaaa aggatagcta tctttgtatg tgatgaccca
    29101 aaatgcttac atatggtata atggtgagtt ccatcgtgcc gatagtatgg tattggccgt
    29161 ttcaaaccgg tctttccgtt acggcgatgg tctgtttgaa acaattcatt gttacggaac
    29221 tgagccttac catctggaat tacattatga aagattgacc aaaggattac aaattctgga
    29281 gattgaaaaa ccctcttcca tatcaaaagt taaacttggg gaggtaatca ccaagttact
    29341 caataagaac cgccattttg ggagcacccg agtaagagtt acagttttta gaaatgggga
    29401 ggggctttac acacccgaaa gtagcgatgc atcaattatt gtggagagtc agcccctaga
    29461 ctttaagttt tacgcttaca accaacaagg tttaatagta gatgtttacc ctgatattaa
    29521 aaaatcggta aatattctat caacagtaaa aagctgtaac gccttacttt acgtgaaagc
    29581 gggactttac aggaaaagaa atttactcga tgagtgcatt attctcaacg agtttgggcg
    29641 ggtagctgag actatttcat cgaatgtctt tgttgttaag agaggcgaga tatttacacc
    29701 agggcttacg gaggggtgta ttccgggaat tatgcgcagg gttgtaataa accttgcccg
    29761 cagtttaggg tataaagttt ttgatgatgt agccctaaac accaaggttt tcagcacatg
    29821 cgatgagttc tttattacaa acgcaattag tggaatccgt tgggttcttg gacttgggag
    29881 caatcgctac tttaacactg tgtccaggca cctcagcatt gagctaaata aagcaacctt
    29941 taaggattta gtttagcgat ggattttcag gatatatagt ccaaacctta aacttttcgc
    30001 ccagttgctg aacggcaaag taccagttat cgtaaacccc tttcattaca tcggttggcg
    30061 caaaattggc cactacccac gacttttcct ttatctcatt ctccagttgc tttggccccc
    30121 agccagagta accaataaaa atccttaaat tcttggcgtt taccttaccg gcaatggcaa
    30181 gcgattttaa tgcgttgata tcgccattca tgtaaattcc atttcccagg gggtatgaat
    30241 ttggtattag atcgccaaag gtgtgcagaa aattaatggt ttcagggcta accggtccgc
    30301 caaccgacac gttcacatca acatccggaa aatcgaccaa tatatcgcta aggctgatgt
    30361 ttagcttgcg gtttacaata aatcccatgc agccatttgc atcgtgttca accaggtaga
    30421 ttactgtccg cttaaaaaac ggatcgttca gggatggctg ggcaattagc actctgccgg
    30481 atttaggtgg aatttgtttg aacttaatat caaacaggtt gatatcgatt tcttttcctt
    30541 ttgccataac cacctccttc ctgatttgtt tcactcttca actatccaaa aatatgccaa
    30601 atttttcttt tgaattgctt ttccataata attttcctga acgattttta aatgaacgca
    30661 tcctttgtat gttaatgctc ggggacgatg gagttcatat caggattatt gctattttcg
    30721 aaaaatttta attgggtatg ataaagcaac caaaactcat tcatggcatt attcccattg
    30781 catttctgat tctttttctc actttgaatg tgatttactt tggcgatgaa acgctatcgg
    30841 gagctaacca aatagcattg ctgcttgctt caactatagg ttccgctatt gctattcatt
    30901 tgggctataa gtggagttac attaggcaaa tcattgtaaa gaccatagga tcggccatgc
    30961 catcaatact aatactaatg ttgattggtg cactggctgg tacatggatg attagcggcg
    31021 ttgttccaac actaatatat tacggtttaa aaatccttca tcccaaaata tttctgtttg
    31081 ctacggttgt tattacagca ttagtttcac ttgcaacggg gagttcttgg tcaacagttg
    31141 ccaccatggg ggtggccttg cttggaattg gcaatgcgct aggctttaat cacgccataa
    31201 ttgctggtgc aatagtatcg ggagcctatt ttggcgacaa aatgtcaccc ttgagcgata
    31261 ccactaacct ggcaccagcc atggccggaa cggacctgtt tacgcatgta aagtatatga
    31321 ccattacaac aattccttca ttagcaatta cccttatcat ttttctggtt ataggactta
    31381 ggtttgaacc acaaggcgtt gttgatgttc aaaccacaat gaatacccta acctctacct
    31441 tcaacatctc accatggctt ttgcttgtac ccatcttttt gattgtggta attgttaaga
    31501 aaacgcctcc tttaccatca attatgtttg gtgttttgct tggtggtgtt tttgctataa
    31561 tttttcaacc tcacatagtt gagcaggtat caggagttac agataactac ccggaggccg
    31621 gctatatagc agtgatgaaa agcatgtttg ggaaagtaac aatcaattcg ggcgatgctc
    31681 aaatgaacga gttgcttaca acgcgcggca tggctggcat gatgaatacc atttggctta
    31741 ttataacagc aatgattttt ggtggtgtta tggaatcggc aggtttgctg gtgaagataa
    31801 ctgaaacaat aattaggttt gctaaatcaa ctgcaagcct ggtggcctcg gttgttacta
    31861 gctgcctatt cttcaacgtt acagcatcgg accagtacat ttccattgta gttcccggcc
    31921 gaatgtttgc taaaacattc cggaaaaggg ggtataaacc agaactacta agcagaacgc
    31981 tggaagattc tggaacggtt acatcggtgc ttatcccatg gaatacttgt ggtgcaaccc
    32041 aagccgctgt gcttggtgtt ccaactttta gctatgcgcc ctatgcattc ttcaatatta
    32101 ttagcccctt tatgagtgtg tttattgcta caatcgggtt taagataaga agattcactc
    32161 ctgaggaaat gcaggaaata gagcgggaag acgagaactc gccagtggag ttaaagtttg
    32221 agcaggaata gatattaaaa aagcccggga aactgggctt tttttgctta gaatagttta
    32281 gggtagcgtt tggggttaac ttcgtgcatc aattcgtata ttccatcaaa aacatcctca
    32341 gcattgggat tcgagaagta gtcgccatcg gttgtatagg ctggacgatg ctcacgtgct
    32401 gtaattgttt ttgggggtga atcgaggtat tggtatcctg ccctctcttc aagtaccttt
    32461 tgcatcatgt aggctgttgc acctccagga acatcctcat caaagaatac aattcggttt
    32521 gttttcttta ttgaatcaag aattaggtta ggtaaatcga aaggtaagag tgtttgaaca
    32581 tctatcagtt caacagaaat tccaaactcc caaagctgtt caacggcttc ctgggctata
    32641 cgaacgcagc tgccataggt aactagtgta acatcggaac cctcgcgaag aatctcggga
    32701 actcctaatg ggatcttata ctcaccaata ttttcgggtc gtttttcctt taagcggtaa
    32761 ccattcaagg gttcaattac cagtgctggg tcatccgctt cgagtagagt gttgtaaaag
    32821 ccagcagcct gagtcatatt gcgaggaacg caaacgtaaa caccccttat tgagttgata
    32881 accatgctta aaggtgagcc agcatgccaa accccttcga gcctatgacc tcgggttgaa
    32941 ataataaggg gcgccatttg gccacttttt gttctccacc gtatggtggc caggtcgtcg
    33001 ctcatggttt gaagcgcata caggaggtag tcaaagtact gaatttcagc tacggggcgc
    33061 attcccctaa gggctaggcc aagcccttgt ccaagtatgg tattctctct aattcctgta
    33121 tcgcttatgc gaatttcccc atacttcttt tgtaatcctt cgtacgattg gtttacgccg
    33181 ccaattcctc caacatcctc accaaaaata accagaaggg gatttttctg aaactgggca
    33241 tcccagttgt cgcgaagtat ttcacgtccg gcaaccaaag gtgaatcatc cttgaaaacc
    33301 ggcggaattc ctttcacttt ccatgcggct cgttcggtct cgcagtaaag atgcgagctg
    33361 tacctgtcgt aaccatcgtt catgttgcgt tcaatccagg cggttaagtc ggcctgaagc
    33421 ttttcgcgta tactgcaatc ggagcaaaca tggcggagaa tgcgtttagc tgcgctaata
    33481 ttatccttgc ggatagggtt gggtattgcc ttaagctgtt cagttatttc accaacctta
    33541 tcaatgtgct cgcgcttgca gaaacatgaa cggttatcaa taattttaat cagctcatca
    33601 cgttccttct tgataggatt cgtaaactca gcccacgcct tttcgcgtgc agcttttgca
    33661 tcggcttggg ctttagcttc aatttcgttg agttcggttt cggtggcgta gccttcagta
    33721 acaatccaat tacggaaacg tgcattgcag tcgtaatcct tttcccattg caagcgctca
    33781 ggcgatttgt accgctcatg cgaacccgat gtggaatgtc cttggggttg tgtaatttcc
    33841 gttacatgga aaagaacagg gacctgttcg gttcttgagc gttcaactcc ttgtgtaaat
    33901 gtttcaatca gagctggata gtcccaacct tttacacggt atatatatat tccgtttacg
    33961 tcggattcgc ctttctcaaa gccttttaca agttctgaga tatttccctt agctgtttgg
    34021 taatccctag gtacagagat tccatagcca tcgtcccaga ctgcaacaac cattggaact
    34081 tgaagcactg ccgcagcatt gagggtttcc caaaaatgcc cttccgatgt gctggcatcg
    34141 ccaattgtac cgaatgcaac ctcgttccct ttacggctaa attgctcaaa tcgccaaagt
    34201 tccttgttgt tcctgaaaag ttttgatgcc caggctaacc caagtaatcg gggcatttgg
    34261 ccagctgtag gggatatgtc aggtgtgcta ttcttttgct ttgttaaatc cttccatgtg
    34321 ccatcgggat ttaggctgcg ggtggcaaaa tgattgttaa aggttctacc tgcattaccc
    34381 gggttaagtt caatatcggt ttgaccgtaa agctgggcaa aaaactcctt agcggtaaac
    34441 agtcctgccg ccatcataaa ggtttggtcg cggtagtagc ccgaacgcca atcgccttcc
    34501 ttgaaggttt tagcaagggc aatttgagca agctctttac catcgccaaa aattccaaat
    34561 tttgctttac cggttaacac ctccttacgc cccataaggc ttatattgcg gcttaggtgg
    34621 gcaatgtagt agtcgtttaa tatctcttct ttactaaatt taattttggt tgtgctcatc
    34681 gttcaattct gttgagtttc atcaggtaaa caaaaaagtt gtgcaattgt tttgaaccca
    34741 aatcttaatg cccgactttt gattttttaa gctttttaat aaaacttttt gtgtttctat
    34801 ctgttagttt tttgcgcgag caggctaata ggtatacttt tgcaaaaacc tttacttaaa
    34861 tgttaaccct aaaattaatt cttgttgcag ccacaattct tggaatagcc ttattgggga
    34921 tggctgttag aataattttt cataagagcc ataagtttcc ggaaacatcg gctggacata
    34981 ataaggaatt gcgaaagcgt ggtgttacat gtcctcgtca cgaggaaatt aagtgctggg
    35041 ggaaaaacgg caaaaatacc gattgcgcaa cctgttacga gcatgcccgc gaggttgtaa
    35101 actaaaacta aacattatgg cattgcttaa acttattggc gtagttgcat tccttttagg
    35161 aattgtaatt attgggttat cgcttaaaac aattatcaag tcacgcaaat ccgggtgcaa
    35221 cgattccgat attggttatt cctgtggttg tggcggtggt acaaattgca gcactaacgc
    35281 tgcaagtaag gggatttaat taaggtataa ttctcttttt cataccttcg ttcatccaaa
    35341 caaggtaatt cccaagcgaa tcggagtaaa caaggtaagc gtcaatatcg ttgctcttga
    35401 caacaaagtt tttgctccat tcaacacctt taaccatgaa tgcggttgcc agagcatcgg
    35461 ccttggtagc actgggtgca actacagttg cactcaacaa gctgttcagc tcagggaatc
    35521 cggttttggg attgatagtg tgcgcatact tttgtccgtt tacctcgata aattttcggt
    35581 aattacctga ggttgctaac ccatagccat tgctaattga tatttttgct tgaatgttct
    35641 cgcctggtag agcagattct acaggcttat cgataccaac aacccaatcg gttcccttcg
    35701 aattcttacc gcgagcagta atttcgccac ccacttcaac aagataatgc ttaataccat
    35761 tactctccaa atacttacca attacatcaa ccgtatagcc ctgcgctatg gcatttacat
    35821 caatctgaat tctgctatca tcctttttaa gggtattacc gtcaaggtgt attttgttca
    35881 tcccaattaa agggagtaac tgttttacct tagccgagtc aataccttgt gttttatcct
    35941 tgccaaagcc tatggctcta acaactgggc ctacggtaat gtcaaaagct ccatcgcttt
    36001 ggtggtaaat ttcaaaagag cttactatta cttcctttag catatcgtca atactatcgg
    36061 aaatattact gttcaaaaga ctaattatgg aactgtcgtt gtaaatggac attgaatggt
    36121 caattcttcc aagtattgaa tctatttgaa ctgaaaaatc gcggataagg gagtcgtaat
    36181 aggttatact ataggtagtc ccttgagtaa aaccttcaat ttttaagtat tcttttttgg
    36241 gtgagcagct tactaaaata atcactgcca gtgtaaatac agttaggtat cgcatagctc
    36301 aattttttac aaatgtaacc ctaaagccta aactcctgaa caaccttttg gatacttttt
    36361 ttgtaaattg ccaaaaattt gcgctatgtt gaatttgtta ggtgatcagg catatcttaa
    36421 ctcttaccag ctcgatattt acgatacgtc gttcgacaag ctgatgcaga aacgcattta
    36481 caaggtttta cttatctgca gcagttacga tgcattcctg cttgaagaag acggaaggat
    36541 agatgaacaa atctttaatg aatatgtctc gttaaatctt cgatatccac cggttttcct
    36601 tcaagcccat agtgcccgtg aggttctccg tatactccag gaagagcata tcgatttaat
    36661 catttccatg cttaacgtgg gtgagatgga tacctttaac ctggcaaaaa tgctaaagca
    36721 gcgctatcca tccatcccta ttgttgtcct tacacccttc tcgcgcgaag tatcattgcg
    36781 attacagaac gaggatttaa gtgctatcga ttacgttttt tgctggctcg gcaatgccga
    36841 tcttcttttg gctatcatta agcttattga ggataagatg aatgccgagc acgacattct
    36901 tgaaattggg gttcaggcaa ttattctggt tgaagattca gtaaggtatt actcctcgtt
    36961 tttgccaaac ctttacaaga taatctttag gcaagcccgc atgtttatga ccgaagcgct
    37021 aaatgagcac catagaatgc ttcgtatgcg cggacgaccc aaaattctat tggctacaac
    37081 ctacgatgag gccatagagc tctatagtaa gtataagaat aacgtgcttg gcgttatttc
    37141 cgatattagc tataaggtaa acaataagcg cgaccatgtg gcaaaggctg gactacagct
    37201 atgcagacac attaaagccg atgatcaaaa cattccgttc ttacttcagt cgtccgagct
    37261 ttcaaataaa atatatgctg atgagctttg tgtaggtttt ttacacaagt attccaaaac
    37321 ttttagcctt gaccttcgcg attacattgt aaacaatttt ggatttggtg attttgtttt
    37381 tcgtgacccc gatacccttg ccgaaattgc ccgcgcatcg gacttgcgcc agctacaaaa
    37441 gctaattctc accattcccg atcgctcatt ggtttaccat tccagcagga acgacatctc
    37501 caaatggctc aatgccaggg ctatattttc aattgcacag ctctttaagc ccatgcggct
    37561 taaggatttc agaagtatag atgatgcccg aaactttatt tactatgcca tatcggcata
    37621 taggctaagc aagggtcgta gcataattgc caagttcgat agggaaacct ttgacgagta
    37681 taagtttttt tcacgtattg gcgatggatc aattggggga aaagcccgag ggttagcttt
    37741 tgtaaaccag gtaatccgga actataatct tttcaataag tttgaaaaca ctataattac
    37801 cattccaaga accgttgtgc tcagtactga tatatttgac gagttcatgg agtctaataa
    37861 cctttactct attgctttgc aagatttgga tgatgaggtc atccttgaag ccttcttgaa
    37921 agctaaacta cccgagcgta taaaaataga ccttcggaaa gtcatttcag tatttcaaag
    37981 gcctctcgcg gttcgttcct caagcaaact tgaggattcc cattatcaac cgtttgctgg
    38041 aatctactca acctacatgg ttccccattc cgataacccc gatcaaatgc tccaacactt
    38101 agccaaagca gttaaagctg tgtatgcatc ggtattctat aaggccagca aggcgtatat
    38161 gaatgccacc caaaacgtga ttgacgagga aaagatgggg gttataattc aggagatctg
    38221 tggaaaccag tatggtaaca tgtatttgcc aaccctttcc ggggtagctc gcagtatcaa
    38281 cttttacccc attggttccg agaaaactga ggatggtatt gttagcgtgg gttttgggct
    38341 tggcaaactt attgttgatg gtggggtagc gcttaggttt tctcctaaat atcctaaaaa
    38401 gatactgcaa ctttcatcgg ttgaaatggc gctccgccaa acccagaaga acttctacgc
    38461 ccttaatgtt gatcccaaca gctttgaacc aaaggttgac gatagctcaa atttgctaaa
    38521 tcttgatata agggaggcct cttctatacc cgatttccgt ttaataacat ctacctacga
    38581 tatgcactca ggagtacttc gcgatgggta ttacgatgag ggtttgaagc tggtaacttt
    38641 tgctccaatc ctaaaacacg ataccttccc actggccaag attttatcgg aactcctcga
    38701 gataggcgag cgtgaaatga ataatcatat cgagattgag tttgcagcaa acctgaaccc
    38761 cccaagcggt atgcctcgac ttttcaactt tctgcagata cgccctatag ttgatagcga
    38821 tcaaacggag ctggtagagt ggaacgagat ggatataaat aaggccatac tctactcaaa
    38881 gtcggcattg ggacacggaa aggttaagga tatatatgac tttgtttaca ttaaaccgga
    38941 aagctttaat ccggccatga ccaaggaaat tgctcatgag ctcgatgagc tcaataaaag
    39001 gtttatcgat ttgcaaagga attacgttct ggtaggccca ggccgctggg gtagtagcga
    39061 tccatggttg ggtgtaccta tcaaatggtc acaaatttca caggcgcgtg ttattgttga
    39121 ggccggtctt gagaacttca gggttgatcc tagccagggt acccatttct ttcagaacct
    39181 tacctcgttc agggttgcat accttaccgt taacccttac ttacacgatg gttcgtacga
    39241 cattcagttc ctcaactcta ttcctgcatg gttcgaaaca aaatatatac gatgcataag
    39301 gtttgaacaa ccgcttgtaa tacaggttga tgctaaaaca aaccaaggtg ttatattaaa
    39361 acctgaaaac ggttgattgg ttttaatttt taagtaatac gccaaaaaaa tgtttaatat
    39421 catatttttt catggttttt ttcttttgaa atgtcatttt aataaatcaa ttttgaaaaa
    39481 ctttttaagg ggataattcc tcgcaataag aaaactgagt ttaacaacta aaaacagtta
    39541 gcgcaatgaa tgtaaataaa ttaatggctg agcttgaagc taagcaccca ggtgaggtgg
    39601 aatacttaca agcagttcgt gaagttcttg agtcaattga agaggtttac aaccagaatc
    39661 ctcaatttga atcggcaaag attattgagc gtatggttga gcctgatcgt attttcacct
    39721 ttaaggtgcc ctggatggac gatgagggta atattcacgt taacctgggt taccgtgttc
    39781 agttcaacaa tgccattgga ccttacaaag gtggccttcg tttccaccca agcgtaaacc
    39841 tttcaatcct taagttctta ggttttgagc aaattttcaa gaacgccctt acaacccttc
    39901 ctatgggtgg tgctaagggt ggtagcgatt tcaaccctaa gggccgtagc gatgctgaaa
    39961 ttatgcgctt ctgccagtcg ttcatgcttg agctctggaa gcatattgga cctgaaaccg
    40021 atgtacctgc cggtgacata ggtgttggtg gccgtgaaat tggttacttg tatggaatgt
    40081 ataagaaact tgccggtgag aacaccggtg tactaaccgg taagggtatt aactggggtg
    40141 gatcacttat ccgtcctcaa gctaccggtt ttggtgctac ttactttgct caggaaatgc
    40201 ttgctaccaa aggcatgtcg tttgcaggca aacgcgttgc aatttctggt tttggtaacg
    40261 ttgcttgggg tgctgcccta aaggttaccg aacttggtgg taaggtggtt accatttcgg
    40321 gacccgatgg ttatatcctt gacgaagctg gtattagcgg cgaaaagatt gattacatgc
    40381 tagagcttcg cgcttcgaac caggatattg ttgagccatt cgccaagaaa ttccctggtg
    40441 ctaagttctt cgccggcaaa cgtccttggg aagtaaaggt tgatgttgct atgccttgtg
    40501 ctacccaaaa cgaacttggc aaggccgatg ctgaagccct tgttaagaac ggtgtagttt
    40561 gcgttgccga gggtgctaac atgccttgta ctcctgaagc cattgaggta ttccagcaca
    40621 acaagattct ctttgcccct ggtaaagctg taaacgctgg tggtgttgct acttcgggtc
    40681 ttgagatgac acagaatagc atgaaactca actggccaag cgaagaggtt gatgctcgtt
    40741 tgcaccaaat tatgaagagc attcacgagg cttgcgttaa acatggtact gaaaaagacg
    40801 gttatgttaa ctacgttaaa ggtgccaaca ttgccggttt cttgaaggtt gccaacgcca
    40861 tgctcgatca gggtttaatc taagcaaaac caaatgtttt gttaacttta agggctcagc
    40921 aatgagccct taattttcaa acacgtatgg tatgagcact acatctacaa ttatctataa
    40981 gggtgattta cgaaccgagg caactcacac acgctccggg gtaaccatta caaccgatgc
    41041 tccagaggac aatcagggta aaggtgagtg tttttcacca accgatctgc ttgctacttc
    41101 gttgggttgc tgcatggtta caattatggg aattgcagca cgtacccacg gatttaatat
    41161 tgatggcaca aaaatagatg tgcaaaaggt tatgggaaca aacccacgta gggtggttga
    41221 ggtaataatt gacctatact tcccgcacaa caactatact gctaaagagc gaaaactgct
    41281 cgaggcttca gccaaggagt gccctgtggc tcaaagtctt catcccgatt taaagcaaac
    41341 aatccggttt catttcccgg aataagaaaa cgcccgctag ggcgttttct tttgttatag
    41401 actttgtttg ctttctgcgg aaatgtcctt gtttactttc ttgattaaac cctgaagtac
    41461 gctgccagga cccaactcaa caaattcggt agcaccgtcg gcaatcatgt tttgaacggt
    41521 ttgtgtccac ctaacaggtg cagttagctg ggctacaaga tttttcttta tctcctcagg
    41581 acttgaggtg ggtttggcag ttgcattctg gtaaatggga caaacaggtt ttttaaatgg
    41641 agtagcattg atggctgctt cgagttcctg gcgtgcaggc tccatgagtg gcgagtggaa
    41701 tgctccgcta acggccagtt taagtgccct ttttgcaccg gcctctttta gtttttcgca
    41761 ggccatatcg acacccttta ccgaacccga tataacgagt tgacccggac agttatagtt
    41821 ggcagcaact acaacttcgt taatggaggc gcaaatctct tctacttttg catcatcaag
    41881 cccaagcaca gcggccatgg ttgagggctg aagttcgcaa gccttttgca tggccattgc
    41941 acgtgccgat actagcctta aaccatcctc aaaactcaaa gcgcctgctg ctactaacgc
    42001 tgagaattct cccaatgagt gacctgccac catttcgggt ttgaaatcgt tccccaaaac
    42061 ctttgcaagt ataacagagt gcaggaatat agcaggttgg gtaactttgg tttgctttaa
    42121 atcctcatcg gttcctgcaa acattaaatc ggtaattcta aatcctaaaa tttcatttgc
    42181 tttttcaaaa agttccttgg ctacggatga actatcgtac aaatctttac ccataccaac
    42241 aaactgggcg ccttgacccg ggaaaacata agctttcata gttaaaggtt atagtttatc
    42301 aaaggcaaat gtaaggcatt ttttaatttc tgtgaccatg aaacctgaaa ttgtaatggt
    42361 tcattgacct taggtgatgc ttaccatcct aaagatcaat taccatttcg cctatgggaa
    42421 ggtcttgtaa aacaactttt ttatcgagca gcaaaacttt accatccgga taaatatcaa
    42481 atgctgcgtt gtatagtgag cgatcggcag taatgttaca gtatatcgtg aactggttgt
    42541 ttgatagggt taccactggg gcttgaattt gatggcttat aacatcgaaa agctctaatt
    42601 caggaaattc aatgaaatca agatcgttaa cagtttctac tattttttga tactggtaag
    42661 gaaaaaggta ggttgagtag taaagtttaa ggtaatcgag cacattctta atgttaaggt
    42721 ttagtctttc attcattagt aatgagttcc attgtcctaa accgcttaat gggtaaacct
    42781 gaactccatt atcaaccacc attagttttg atgccggaat acaatcataa tcggttatgg
    42841 aaagtagctt ggagtttggg aagaatgttt gctcaattgg ttcaacagag caattcttgt
    42901 taaaggttat gtatttgcct agttgggcat ttacattgcg tactatgcgg gtatataaat
    42961 ccttatcaat ttttgaaatg cccatacttc taatttacca cttgcaagat attggtaaat
    43021 acgatttaag tcaagagtct tataaaaata tttgttaaca gtttgacggt aatttttttt
    43081 acattaacct tgtaagaaaa ttaacaccta aaaccatgaa aaaattatta gttattctaa
    43141 caattttagc aacaaacttt tatactgcca atgctcaact atttaaggta tttggccatg
    43201 atgttgggtt tatctacgtt ggaccaaaag tgggtatgaa cttttcaaaa tttagccaat
    43261 ggtcatatac cggctatgat gtgaaaaata gaattggata tcagctaggt gttgttggtg
    43321 attttgggtt tacgaatagg ttttcctttc aaacagagtt gaactttat
//
LOCUS       DAOSAL010000015        41307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_716_length_41307_cov_48.556725, whole genome shotgun sequence.
ACCESSION   DAOSAL010000015 DAOSAL010000000
VERSION     DAOSAL010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 41307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41307
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_716_length_41307_cov_48.556725"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..219)
                     /locus_tag="PK049_03745"
     CDS             complement(52..219)
                     /locus_tag="PK049_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60704.1"
                     /translation="MAIGLSIGLVLGAIAGITIGVLLHDIALWLSIGIGSGMAFGIAI
                     GVLIGSRKKFE"
     gene            complement(297..1787)
                     /gene="glpK"
                     /locus_tag="PK049_03750"
     CDS             complement(297..1787)
                     /gene="glpK"
                     /locus_tag="PK049_03750"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HOK60705.1"
                     /translation="MEKFIMALDQGTTSSRALIFDQKGNIRSMAQKEFQQIFPKPGWV
                     EHDPNEIWSSQVAVAAEAMSKIGINGQNLHGIGITNQRETTIVWDRSTGEPIYNAIVW
                     QDRRTSSLCDELRAKGFTDTIRQKTGLVIDAYFSGTKVKWILDNVPGAREKANRGELA
                     FGTVDTWLVWKLTRGRTHVTDVSNASRTMLFNINTLTWDKELLDLLQIPESILPRVAS
                     SSEVYDHTNTTFFATKVPIAGMAGDQQAALFGQMCTSQGMVKNTYGTGCFILMNTGKK
                     PFLSKNNLVTTIAWQVNGDTYYALEGSIFIGGAVVQWLRDGLGIIKSSAEVEELARQV
                     PDNGDLYLVPSFTGMGAPHWDQYARGLMIGISRGTTRAHIARAALESIAFQTMDVLDV
                     MAKDTGINLTELRVDGGASANNLLMQFQSDVLGINVVRPKTIETTAMGAAYLAGLATG
                     YWKNIEEIANLWQLDKTFTPGMPAETLQKMKDKWADAVERAKSWHR"
     gene            complement(1828..3393)
                     /locus_tag="PK049_03755"
     CDS             complement(1828..3393)
                     /locus_tag="PK049_03755"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HOK60706.1"
                     /translation="MNREQMLSQAVSNDQGFDFVIIGGGATGIGIALEAATRGYRTLL
                     LEKHDFTKSTSSKSTKLVHGGVRYLAQGDIALVREACIERGRLLKNAPHLVKNQSFVI
                     PTFGWFDELMYTVGLTFYDLLAGSYSLGRSLRVSKKRALEYIPTIEPKKITAGVVYHD
                     GQFDDSRLAINVLQTATEHGAMVLNYMNVVKLTKNTNGIVDGVVAEDLETGRSYTFHG
                     KAIINATGVFADDVLQMDIPGQAKTISPSQGVHVVLDKSFLPGNHAMMIPKTDDGRVL
                     FAVPWHNRIVVGTTDTPIPNASLEPVALEEEIEFILNTAGRYLTKAPKRSDVLSVFAG
                     LRPLAAPTGESKKTKEISRSHKIIVTQSELFTMVGGKWTTFRRMAEDMVNRVESVKGW
                     EKSKTKTRNLKIHGYTNNVNHSDPLYVYGTDKEKILELARVEPELGETLSQSLGIIKA
                     QVVWAVRNEMARNVEDFLARRTRCLLLDARESMRIAPAVAAIMARELNRDKNWEREQV
                     ENYLAIAQNYILS"
     gene            3519..4310
                     /locus_tag="PK049_03760"
     CDS             3519..4310
                     /locus_tag="PK049_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HOK60707.1"
                     /translation="MFEIKLVGMTLSIAERHRKILEKLKETGYVSVVELSEQLGVSAV
                     TIRKDLKLLESRNLLFRSHGSASVNNPYIADRHVIEKEQLYVEEKQRISRYAASLVSD
                     GESIILASGSTINEFSRQIGERRNITVICASLVAARELAVRGVAEVLQLGGLVRHTAT
                     SVVGPFAEKMLEGFRCSKLFMGVDGIDPEFGLTTTNALEASLNRQMIASAEKVIVLAD
                     SSKFGRRGFSRICQLEQVDLIVTDKGVNELFVKQLEEKGVEVVLV"
     gene            complement(4318..4968)
                     /locus_tag="PK049_03765"
     CDS             complement(4318..4968)
                     /locus_tag="PK049_03765"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HOK60708.1"
                     /translation="MKVHKEGYSILFVTLAVLVVLCAVSWLILPKAISIVATVASVLL
                     MAFLTRFFRVPSRHIETRLNAVLSPADGTVVTIEEVEENEYLKTRCIQVSIFMSVWNV
                     HINWFPVSGVIKYYKYHPGDYLVAWHPKSSSHNERTTVVVERPDGVKVLLRQIAGAVA
                     RRIVCYAEVGKPVNQCSELGFIKFGSRVDVFLPLNADIKVAIGQKVTGNLTVIATV"
     gene            complement(4983..5810)
                     /locus_tag="PK049_03770"
     CDS             complement(4983..5810)
                     /locus_tag="PK049_03770"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-archaeol synthase"
                     /protein_id="HOK60709.1"
                     /translation="MSEFLKRTISGLLFVIVLVGAIYIGHITFFILFLAITTATMLEF
                     YHLGLKAKLRPQSLLGVFIGMAFFIWSYFYSSGRIEQLTLYGFVPLLVSIFVVELYRH
                     QQKPMQNIALTLLGIFYIALPFSLMNFITINGSSYSMDYNPNILLGILFLVWANDTGA
                     YIVGVSMGKHKMIPRISPKKSWEGFVGGIATTLLVAWVIAMFFDEVSLKHWLAIGLIT
                     TFMAVLGDLVESMFKRSIGVKDSGKFLPGHGGLLDRFDALLMVLPMVYVYLEVMMII"
     gene            complement(5807..6010)
                     /locus_tag="PK049_03775"
     CDS             complement(5807..6010)
                     /locus_tag="PK049_03775"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HOK60710.1"
                     /translation="MDQNWQEVFSTSHTWQAEMAKQILEENEIPAVVVNRKDSSYLFG
                     EVAVYTEPENAERAKELLKDIQG"
     gene            complement(6014..8035)
                     /gene="ftsH"
                     /locus_tag="PK049_03780"
     CDS             complement(6014..8035)
                     /gene="ftsH"
                     /locus_tag="PK049_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOK60711.1"
                     /translation="MAENNNNNPMMNDENGKPKMPRFNVYWVYIIIILGFFLIQYFYS
                     GKTAQLTTWQEVKNQMLEKQEISKLLVVRNAGKVEVYLKEDKLEKYKDRLGQGFGAVP
                     KAGPHFYFTIGSIETFERQLAEAQTNIEESQKIYPEYVDRPNYWADILSWVLPFVILA
                     AIWIYIFRRISRGGGGGPGGGNIFSVGKSKAQLFDKETRPRVDFKDVAGLEEAKIEVM
                     EIVDFLKNPKKYTELGGKIPKGALLVGPPGTGKTLLAKAVAGEANVPFFSMSGSEFVE
                     MFVGVGASRVRDLFRQAKEKAPCIVFIDEIDAIGRARGKNPSFSGNDERENTLNQLLT
                     EMDGFASNQGVIILAATNRADILDRALLRAGRFDRQIHVELPDLKERLDIFKVHLRPL
                     KLEENLDISFLAKQTPGFSGADIANVCNEAALIAARKNKKYVEKQDFLDAIDRIIGGL
                     EKKNKIISKEERRTIAYHEAGHATVSWLLEHANPLLKVSIIPRGRSLGAAWYLPEERQ
                     ITTTEQLFDEMCSTLGGRAAEEINFNKISTGALSDLERVTKQAYAMVAYFGMGKNLRN
                     ISYYDSTGQVEFTFSKPYSEKTAEQIDEEVKEIIDKAYERAKQILTENHEGHVQLAEL
                     LLEREVIFSEDLEKIFGPRKTLSREQELVKELDEEAHKQNSESKENQTE"
     gene            complement(8067..8447)
                     /gene="rsfS"
                     /locus_tag="PK049_03785"
     CDS             complement(8067..8447)
                     /gene="rsfS"
                     /locus_tag="PK049_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOK60712.1"
                     /translation="MAVKRVKKTSNTEDLLTCIVDAIQEKKGKNVVSLEIGALPNAVC
                     KYFVFCNADSTTQVDAIANNIEDKVLEKLGEKVYRSDGYQNAIWIVLDYIDIVVHVFQ
                     TEWRNYYRVEALWADAKAIQYEEV"
     gene            complement(8595..8667)
                     /locus_tag="PK049_03790"
     tRNA            complement(8595..8667)
                     /locus_tag="PK049_03790"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8632..8634),aa:Gly,seq:ccc)
     gene            complement(8721..9617)
                     /locus_tag="PK049_03795"
     CDS             complement(8721..9617)
                     /locus_tag="PK049_03795"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOK60713.1"
                     /translation="MPTKKDKSPDILLVPVDFGDYYEKAIEFAQKLAPITKSQIHLLH
                     VLDLKDWWLDEVKEERLINEAFNKLLNISRQFGLPEDSKLMVQTGKRHHKIVEYAEQE
                     NVRFILMVDNYPFAQEGKRIGSTLENVIMKATKPVITVKTVPETIFKNMLVPLDLTKN
                     CRVNLFNSVALALKFGSKLNIVSVIFGDIDPEKSRINEKIEKYAAVYAENHIPHTKKL
                     IRKDEDFAYQEILDYADEIKCDSIIIMTHKENVGFDNYVGAFAHHIINEAKVPVISLN
                     NASSTQTTRDLLSSIIDPLNIF"
     gene            complement(9619..10362)
                     /locus_tag="PK049_03800"
     CDS             complement(9619..10362)
                     /locus_tag="PK049_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HOK60714.1"
                     /translation="MKVKLKIWRQKDSRSKGKFEVYELDNVAPDMSFLEMLDSLNQRL
                     VREGKSPVAFDHDCREGICGSCGIYINGRPHGNDHRTTTCELRMRRFKDGDTITIEPW
                     RAAAFPVVKDLVVDRSAFDKIIQAGGYISVNAGAAQDANAIPVPKHNADVAFDTASCI
                     GCGACVAACKNASAMLFVAAKITHLAQFPQGRVEAPERVKRMVAKMDELGFGSCTNTR
                     ACEAECPKGISISTIAKLNREYITAKLKD"
     gene            complement(10393..12312)
                     /locus_tag="PK049_03805"
     CDS             complement(10393..12312)
                     /locus_tag="PK049_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HOK60715.1"
                     /translation="MSKLNAKIPEGPLAEKWTNHKAKLRLVNPANRRKLDIIVVGTGL
                     AGSSAASTLGELGYNVKVFCYQDSPRRAHSVAAQGGINAAKNYKNDNDSVYRLFYDMI
                     KGGDFRAREANVYRAAEVSNLVIDHYTALGVPFARDYGGLIENRSFGGVQVSRTFYAK
                     GQTGQQCLIGAYSALSRQIDKGTVTMYPRREMMDLVVIDGKARGIIVRNLVTGEIERH
                     SAHAVVLATGGYGTIYYLSTLALNSNGSAAWRAHKRGALFANPSFVQIHPTSIPVLND
                     HQCKLTLMSESLRNDGRVWVPKTKGDPRKPNEIPEDERDYYLERRYPAFGNLVPRDVA
                     SRAAKERCDAGYGVGPTGLSVYLDFRDAIQKKGRKYIEENYGNLFEMYEKITGESPYE
                     VPMRIYPAGHYTMGGLWVDYNLMTTIPGLYAIGEANFSDHGANRLGASSLMQTSGDGY
                     FILPYTIADYLADEIKTPKIPTNTPEFDAAEKEVKERIERLMAINGTETATSFHKRLG
                     KIMWDYCGMVRNEEGLKKALTLLDELKDEFWKNLKVPDTASELNQELEKANRVADFLE
                     LGKLMVYDALNRKESCGAHFREESQTETGEAKRDDENYSYVAAWEFTGDGKEPILHKE
                     ELKFEYVKLQERSYK"
     gene            complement(12343..13005)
                     /locus_tag="PK049_03810"
     CDS             complement(12343..13005)
                     /locus_tag="PK049_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HOK60716.1"
                     /translation="MFNSSIGKKLVMSITGLFLMVFLLVHLTVNMLLLVGDGEVFNRA
                     AHFMASNPAIKVIEPLLAIGFVFHIIYATFLTIKNQRTRPIGYSKTTSNGLSTWSSKN
                     MFILGTTIFIFLIIHLANFFWKLKFGTVETITYGSDTMPNAYALVSGLFISYWWYDLL
                     YVIGAIFLGLHLSHGFWAAFQTLGWNGTKWLKRLEVVAYVYAIIIGTGFSIIPIYFLL
                     FR"
     gene            13788..16253
                     /gene="priA"
                     /locus_tag="PK049_03815"
     CDS             13788..16253
                     /gene="priA"
                     /locus_tag="PK049_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOK60717.1"
                     /translation="MTSQLYADVILPLALQATYTYSVPFEMEKMIQPGVRVVVSFGQR
                     RLYSALVYRVHSEKPQVQQVKDILQVIDTTPIVLPSQYELWEWLAEYYMCSLGEVMKA
                     ALPSGLKMESDTYIASGDFDEIDLSDEEMTLIHLLGQKRKLTIQQLTQKSGLKHPMSV
                     IKSMLERKLLFITEDIESDYRPKTENYIALAPEYSDEKKLCELIDSLKRAPAQQRLIL
                     AIASQATTPNDLSTARVLRKTLLEKVDVSASVLKSLIDKGFIVQDEVEISRIDNANSD
                     PPTRNPLALEDFQINALNEIQNQFSSHDVVLLHGVTSSGKTELYIHLINQALQNGKQV
                     LYLLPEIALTTQIINRLRKVFGDRVGVYHSKFSDSQRVEVYLKMLSEEPFDVILGVRS
                     SIFLPFSNLGLVIVDEEHENTFKQYNPAPRYHARDTAIVLAKQVGAKVLLGTATPAVE
                     TYFNAKSGKFGLVKMLERFSGLELPRTIVVDTRDARKRKRMKSHFSDTLLSAIENALS
                     QKEQVILFQNRRGYAPFIECADCGWVPHCEHCDVTLTYHKYTNQLTCHYCGFGMEQMG
                     SCLACGSTNLQPKGFGTEKVEDELSVFFPDAVIERIDLDSTRSRSSYERIISGFEQGK
                     IDILVGTQMVSKGLDFDRVSLVGILNADNMLNFPDFRAFERSFQLITQVSGRAGRKGK
                     QGLVIIQTAQPDHPVIKSVTGNNFFTFWEWQLAERNRFHYPPFYRLVRITLKHKDKHH
                     LEDTAENLAKTLRHSLGENVLGPEPPIISRVQGLYIIEILVKIKREQPLQRIKEFIRY
                     SITMLKQKRETSTVVIAIDVDPY"
     gene            complement(16257..17486)
                     /gene="hydF"
                     /locus_tag="PK049_03820"
     CDS             complement(16257..17486)
                     /gene="hydF"
                     /locus_tag="PK049_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HOK60718.1"
                     /translation="MQKGKETKPHIGIYGRRNNGKSSLINLLAGQNVAIVSDFAGTTT
                     DPVKKSFEITGFGPVILVDTAGIDDVGELGQKRVEKTLQSINHIDLALLVVTSNTWGD
                     YEEMLVNEFIKTDTPYLVVHNKSDIEPASSIFKEKLSGKRHSGFIDISCKSQTQPDEL
                     ISLIKKAIPESSYRTPTLLGDLISYGDIVLLITPIDVEAPAGRLILPQVQAIRDVLDN
                     DAVAVVLKEREVDAFLRKTQVKPALAITDSQIFVKADASIPRDIPLTSFSILLARLKG
                     DFENYLKGTPKISELKDGDRVLLLESCNHHVSCDDIGRVKIPRWISNFTGKQLEYDVV
                     AGLDALPRDIMEYSLVIQCGGCMITRKQLINRLRPAVEAGIPVTNYGMAIAYVQGIYQ
                     RAIAPFTANKESKADYL"
     gene            17607..18542
                     /locus_tag="PK049_03825"
     CDS             17607..18542
                     /locus_tag="PK049_03825"
                     /inference="COORDINATES: protein motif:HMM:NF025729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HOK60719.1"
                     /translation="MVRKTFAFALVIALMGVTMVTEAQVGGFLKDKAQKAVAKSLKRG
                     EDEKKVEEQQQEEQPEKQGYKQPSAADRYMQQKMMGLMGFKNVKFEPAYSFTSSMKMD
                     VESVDSASTEVNKGAYAFYFDKNSQNFAMEFEALNRETGKKEKSLMIFDYKNKAMLIL
                     SDKDGEKSGIAMNFEPDSVQQQQGKDELAEPVPQEDLSAYNMYYKATGRTKDVLGYKC
                     KEYVYENPDGKVELWATNDIKYDYSEAFGHMNGFQYLATGGTAYLLGTVLEMHFRDAN
                     SNARADFWIKEFNPNRSRTLSVADYQVVGLGDKKK"
     gene            complement(18799..20253)
                     /locus_tag="PK049_03830"
     CDS             complement(18799..20253)
                     /locus_tag="PK049_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60720.1"
                     /translation="MRELIKLHLGRLVTVLALLVVLPNALHAQIDEEEYYKGILDTIV
                     EVQNPVYKPVVSLGVGYLSFWGDVRNPISSPLYGNVAGKLNVSNLVGRKHYFKGNIFA
                     IYGDIFAHDPKLSYLMQQQPLPLDDLNNPIYVNSAFKTNIFQLGLSFEYNFGHVFGPA
                     KRFKPYLSVGFSTIFFSPKGNFLNNGSGNFYYFWSDGTMRNFPENGPDAYRARIVRFD
                     SDYETDLSGKDLYKQGSISQQTYSVPIDFGFDFYLSDRVNLRVGAAFNYCFSDKIDNY
                     DSKIAKAQGYPVKNNIKDIFSFTYFTMNFDLFSDPKTILVERVFAMLGEDYDYEIFFS
                     DADGDGVFDRFDECPDTPAGVAVDSIGCPSDTDFDGVFDYMDEEPNTPEGAIVDESGS
                     QVTADVLAAMFNRKSEAVSRKEVRLIPVTHVWSRSLNYTPGMIPEKLKGVDSDSDGYI
                     SFQELMRAVNEFFDGTNNLTLQDLYDLNEYFFAQ"
     gene            complement(20258..21613)
                     /locus_tag="PK049_03835"
     CDS             complement(20258..21613)
                     /locus_tag="PK049_03835"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOK60721.1"
                     /translation="MPSIVTVLYNLVGITLLSFLIGNDVALNVNVPKAVNAGSEFDVE
                     VILKKGNLSGFARLQQDLPMGLTAQLVESSLGDFSFDEQKLRFVWLRLPEGKEMRLVY
                     RVKVDERLKGSFTLKGTFAYVDNNERKTAEFQTSTISINPSTRIDPNLIVDINEFQTI
                     IPPQAPVGFVASNVRCIRQTPIPSGAENDYLVKLLVNKGNAQKFAKIEEDVPEGFTAE
                     AIETKDAVFTFKDQKVKFLWMNLPAEPRFVVSYKLIAPDGKGNITVSLKGTFSYLVGE
                     ATRVIDIIQKDIDLTNIDPAALDGLISSTFSTEGANIISGFTPYSEGGIDIPIEYKKI
                     EDKPKRKAKPEFDMEPFMLMPEKGVYYRVQIAAGHKPIDIKRYFTRYNIKYDVRTEKH
                     EGWYKYSIGSFKEYKEARDFRILIWNSTRINDAFVTAYNNGTRITVQEALMITSQKWY
                     R"
     gene            complement(21801..23471)
                     /locus_tag="PK049_03840"
     CDS             complement(21801..23471)
                     /locus_tag="PK049_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOK60722.1"
                     /translation="MRKEVEIVIENTGQKVRVTPGHSLLEISKIFEIKFDYQILGAMV
                     NNQLKELDYEIFTPKTVRYIDITHPAGMRMYQRSLFFLLQKAVRDIIPGAKVRIEHSV
                     SKGFYCEIDGCNLPLELPLIFNICDRMREIVAADLPFRRDKVLTSDAIKLFRDLGYED
                     KARLFETRPTLYTSVYYLGDMADYFFGSLVPSTGYLKVFDLTKYYDGMLLRVPKRFQP
                     DEVEDVVIQNKMFEIFQEYKDWLEILGVENVGAINQEVAKGNASELIKISEALHEKKV
                     ADIADRIHQQPEPVRLVLISGPSSSGKTTFAKRLAIQLKVNGLKPYTISLDNYFVDRE
                     KTPKDEFGNYDFEALDALDVDYFNNDLVRLLKGEEVEIPKFSFEEGKRFFDGTKLQIE
                     PNGVIIIEGIHALNPALTHLIPNNQKFKVYVSALTSISIDGINRIPTTDNRLIRRIVR
                     DYRYRGYSALDTIGRWESVRRGEDKNIFPFQEEANVMFNSALLYEFSVLKRFAEPIIQ
                     EVPPTRPEYDEAQRLLKFLSYFTPISEREVPPTSIIREFLGGSTFSYK"
     gene            complement(23505..24323)
                     /gene="murI"
                     /locus_tag="PK049_03845"
     CDS             complement(23505..24323)
                     /gene="murI"
                     /locus_tag="PK049_03845"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOK60723.1"
                     /translation="MSGNCNPIGIFDSGAGGLTVLSELVKIMPNEKYIYFADSANCPY
                     GSKPAEEIIGLSETITQFLLSKGCKVIVVACNTATAAAIDHLRSSYSIPFIGMEPAIK
                     PASQNTKTKSIGVLATAGTFKGRLYIETSRKYATDVNVCYQVGEGLVELVESGKADSP
                     EAEQLLHRYIDPMVDCNIDHLVLGCTHYPFLKPVLERMLPEHITIVDPAPAVALQTKR
                     VAENHGLLCNKPFVHSINIEFYSSSNTNTLKNLVGSSLKLNDKIEDVYFANIKL"
     gene            complement(24397..24915)
                     /locus_tag="PK049_03850"
     CDS             complement(24397..24915)
                     /locus_tag="PK049_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019152366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOK60724.1"
                     /translation="MRKILGLAIVALLTFSFSGVNAQNYKFGHINSQELLKAMPDRDS
                     AEAKIRKYAESLEDQIEQLQVEFNKKYQDYLQKRATFTDAIREMKEKELTDMQQRAQE
                     YQQVAEQDYQRYQVETMKPVIDKADEAIKKVAKANGFTYIFDTSSGVLLYFSEQSIDI
                     TPLVKKELGITK"
     gene            complement(24952..25461)
                     /locus_tag="PK049_03855"
     CDS             complement(24952..25461)
                     /locus_tag="PK049_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOK60725.1"
                     /translation="MKRTILILAALTIGLGAWAQKFAYVDTEYILKKIPSYKAAQEQL
                     DKLSEQYQKEIEEKYAEVDKMYKDYQTEKVLLTEEMKKKREDEIIAQERQVKELQMKY
                     FGRDGLLFKKREELVKPIQDQVFNAIKEIAVEGGYAVIFDAAASPNMIYTNPRFDKSE
                     EVLQRLGFK"
     gene            complement(25535..28015)
                     /gene="bamA"
                     /locus_tag="PK049_03860"
     CDS             complement(25535..28015)
                     /gene="bamA"
                     /locus_tag="PK049_03860"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HOK60726.1"
                     /translation="MMRFRLLALAMFFIGVSAFAQEKPFSYELPKRYCVKEITVSGVK
                     FLNPDVLISMTAISIGDTIQVPGEAITKAVKKLWGQGLFSNVDIKASRIEGDDIYLEI
                     SLTEQPRLTTVNFTGIRKGEADDLKDKLKLRSGSQLTQATLENSIIIIKKHYRAKGFL
                     NVDVNPVLENDTIISNGTKLTFNIQKNDRVKIGEITFEGNKAYGQARLRRALKKIHRR
                     DLNIFKSAKFIEADYDESLDNLVAFYNEHGYRDFKILGDSIYTINPKRIGIHFKIFEG
                     PQYHIRKIEWVGNTKLPAEALTAVLGMNKGDVYDKNLLEKRLFTDENSISTIYMDDGY
                     LFFHLEPVEVQIQNDSVDLEMRIYEGDQATVNRVIITGNTKTNEHVIRREIWSKPGYL
                     FSKSEITRTLRELGQMGHFDPEKLGIDPLPNQADGTVDLKYSVEEKANDQLEVSGGWG
                     NKMFVGTIGIRFSNFSVRRIFDKDAWRPIPSGDSQSLAIRASTNGTYYKAFSLSFTEP
                     WLGGRKPTNFSFSLYRTIQSGGVSYFYQSSDKYFRVSGASVGIGTRLKWPDDYFTIYN
                     EVGIQNYKLSNWSGYFIFTDGQSNNLSYKVTLGRNSTDQMIYPRTGSNFSLSLQITPP
                     YSLFNGKDYSKPMTDSEKYKWIEYHKWTGRIQWYIPLVENLVLYTNFQMGVMGYFNSK
                     LGHSPFEGFDLGGDGMSGYNLYGKETIGLRGYQNGSLTPYVRIGESLVGNAHLYDKYT
                     VELRYPISLKPQAAIYLLTFLEAGNAWQNAGDFNPFNVHRSAGVGARFFLPMLGMLGV
                     DWGYGFDKVPGRPDAHKGQFHFIIGMPF"
     gene            complement(28168..28905)
                     /locus_tag="PK049_03865"
     CDS             complement(28168..28905)
                     /locus_tag="PK049_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOK60727.1"
                     /translation="MSLIDKIDRSRVPEHVAIIMDGNGRWAKKRGNQRIYGHKNGVKA
                     VKAATEAAGEIGVKYLTLFAFSTENWNRPKSEVDALMSLLVETINSETDTLLKNGIRV
                     LTIGDQSMLPPDVQRKLNDVITRTANCNTITTILALNYGSRHEITEAVKKIAAQTKDG
                     TISPNQITPEIISKYLYTANIPDPALLIRTSGELRLSNFLLWQLAYTELYFTPVLWPD
                     FTKEDFFEAIVDYQKRERRFGKTSEQL"
     gene            complement(28910..29560)
                     /locus_tag="PK049_03870"
     CDS             complement(28910..29560)
                     /locus_tag="PK049_03870"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HOK60728.1"
                     /translation="MNKISILLFSLTHCCLAIRAGQAQDRRDFGIQAGTTYYYGEFNE
                     VKPLYSPSFAMGVIFRYNINHNYSIRASAIYANVSGSSSSANIIPDPNISFSKNIIGA
                     EAMGEFNFYSINPTSSRKANLSPYVNAGVGLAQMGTSLIFHIPFGVGLKFTPGQRHTF
                     ALEWRFHKTFTDKIDDYSSPNDGRKPFLHNNDWFSFIGLIYTYRIPNNSYICPAYR"
     gene            complement(29560..>30147)
                     /locus_tag="PK049_03875"
     CDS             complement(29560..>30147)
                     /locus_tag="PK049_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HOK60729.1"
                     /translation="GFGGGVGFKVNPFISAQASLNTGFLGNTDDGSYYSSRDYGFSTF
                     YSELTLKGIYYIIPESNQNYYYRIMDLRGGLRHINKPFSLYVFAGAGGLFFSATPNEK
                     LLSRTPPPGFTKPEVDDSKNFTLVIPVGVGAKYEFYPRFQIGVELGARYVTTDYLDAF
                     SSVYSNHNDMYYTINFNVYYKIPYQKLLKRSFWQF"
     assembly_gap    30148..30247
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(30269..31144)
                     /locus_tag="PK049_03880"
     CDS             complement(30269..31144)
                     /locus_tag="PK049_03880"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF002521.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HOK60730.1"
                     /translation="MTIAVYGKRIDAEFAEATRNLFAALEKYRVNVLIYLPLLDYIKS
                     NLSYTPHIGGTFITTQDLKETDVMISLGGDGTFLDSIALVQDMGIPIMGINLGRLGFL
                     ATTSIPEIDNSIDLLMASKYSIEKRSMLQLVSDSKLFDPFPYALNDMVIRNSVAGLLH
                     VNTYINGDYLSTYWADGLIIATPTGSTAYSLSVGGPILTPNLSAFVLSAIAPHHLTVR
                     PLVIPDDSLIELEVSGRDEELIVSLDSRNIRVPQPVKLMLNKAHFNANVICLKGTTFY
                     RTIRSKLLWGMDKRN"
     gene            complement(31149..31814)
                     /locus_tag="PK049_03885"
     CDS             complement(31149..31814)
                     /locus_tag="PK049_03885"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOK60731.1"
                     /translation="MVAKDMISDVIPPLKTSDTGITALNWMDIFKVSHLPIVNDKEFL
                     GLISESDIYDLNMPEEPLGNHQLSLLRPYVIEDQHVFEVMEVLSRLKLSLVPVLDSSK
                     KYLGVITLMELLHYFTELSALRHKGSVIVLEMNVNDFSLSQICQIVEGNDAKILAAYI
                     TSHPNSTQMELTLKLNVTDVTSIKQTFQRYNYNVLGAYMKQDDESDLLNDRLNYLFKF
                     LDI"
     gene            31868..32584
                     /locus_tag="PK049_03890"
     CDS             31868..32584
                     /locus_tag="PK049_03890"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005781628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HOK60732.1"
                     /translation="MTRLSVNINKIATLRNARGGNIPNVLKAAIDCERFGAEGITVHP
                     RPDERHIRYQDVRDIRPVITTEFNIEGYPSESFIELVLSVTPHQVTLVPDPPNALTSN
                     TGWDTIANKDFLKKVIARFQEHGIRTSIFVNADPLMVKHAADTGTNRIELYTEPYATH
                     FPENPAQAIEPFVKAAEAAMEVGLGLNAGHDLNLDNLNYFARNIPNLLEVSIGHALIC
                     DALYYGLENTIQMYLRQLRF"
     gene            32585..33379
                     /locus_tag="PK049_03895"
     CDS             32585..33379
                     /locus_tag="PK049_03895"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOK60733.1"
                     /translation="MELFYRQLGQGKPLVILHGLYGLSDNWISIAKNLESSYSLIIPD
                     QRNHGQSPHSPDHTYQHLVNDLLELTNKLKLDSFYLMGHSMGGRVAMLFQSQYPFKVE
                     KLVVVDVAPWSYSPEDEWFRSAYAEHMRIIEALKALRPDEIASRMDADKVLQQNNLNE
                     RLRNFLLKNLKRKSLGFEWQINLNALENNLEHLLLGVEVDKTSALGANVLFIRGENSN
                     YIPVYQVDRLKEIYPMCQVVSITDAGHWVHAEKPQMFVSAVTSFLG"
     gene            complement(33376..34101)
                     /locus_tag="PK049_03900"
     CDS             complement(33376..34101)
                     /locus_tag="PK049_03900"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOK60734.1"
                     /translation="MLTDVKLDEILFLDIETVPQYPEYNQMPEEFKKLWDRKAKTLAK
                     PDETPEAIYERAGIYAEFGKIICISVGIIVPSNGTNGFRVKSYYGDSEVDLLKSFAEM
                     VNGFSARKPNLNLCAHNGKEFDFPYIARRMLVNGIKIPDILNVAGKKPWEVKFLDTME
                     LWKFGDFKHYTSLNLLSYIFGIPSPKDDIDGSEVAKCYYELNDLNRIVIYCEKDVLTV
                     AQLFLRYNGFPIISPENVSRSEV"
     gene            complement(34104..34727)
                     /gene="udk"
                     /locus_tag="PK049_03905"
     CDS             complement(34104..34727)
                     /gene="udk"
                     /locus_tag="PK049_03905"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HOK60735.1"
                     /translation="MLIIGIAGGTGSGKTTVVNKIIDLLPNGEVVVIPQDSYYRDNSH
                     LPLEQRQEMNFDHPDSIEFELLVQHVKDLKAGKPVEQPIYSYLTCARAPETIKIEPRH
                     VVIIEGILIFTCEELRKLMDIKVFVDADADDRLSRVIARDIVERGRSVNKVLERYEKT
                     VKPMHLQFIEPTKRYADIIVPQGGNNSVAINILTSIIEKTLRSHNLL"
     gene            34814..35809
                     /locus_tag="PK049_03910"
     CDS             34814..35809
                     /locus_tag="PK049_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HOK60736.1"
                     /translation="MSNSLYPLKFKPILKERIWGGTRLHHSMGKQFPNPEEKYGESWE
                     VSGVEGDVSVVQNGFLAGNTLNELIEVYMGDLMGESLYERFGEEFPLLIKLIDASETL
                     SIQVHPDDETARERHHAYGKTEMWYIIDADRDALIYTGFEKDITREEYIQAVKEGNLE
                     RYLHREKAIPGDVFFIPAGRVHAIGKGVLLAEIQQTSDITYRIFDWNRVDGSGKSREL
                     HTELAVDVIDFARVRNPKSHPVDEVNKTSELSACKYFTVNRLTFNNAMQRDYNMLDSF
                     VIYLCVEGECSVVYASGSSERLTKGETLLLPADLKDIILRPQGKCSLLEVYINQA"
     gene            35980..>36194
                     /locus_tag="PK049_03915"
     CDS             35980..>36194
                     /locus_tag="PK049_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60737.1"
                     /translation="MDSVIYFIKSTLANEIAASGFPLIYKGEMNHQIMRSFAFMANRK
                     IAEMNVPTATRKRVFHIMIECLQNITK"
     assembly_gap    36195..36294
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <36295..36710
                     /locus_tag="PK049_03920"
     CDS             <36295..36710
                     /locus_tag="PK049_03920"
                     /inference="COORDINATES: protein motif:HMM:NF038262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HOK60738.1"
                     /translation="RKRVFHIMIECLQNITKHSDDYDEKEKQIGNGLFVVGKERDSFY
                     IVTGNLVRNDKVPILEQRLDRLNSASPSQLKEMFMQQMVEGELNEKGGAGLGLIDIAR
                     KSKRKLYYHFVPYENNRHFFILAVTIPISAEEDDF"
     gene            36889..37794
                     /locus_tag="PK049_03925"
     CDS             36889..37794
                     /locus_tag="PK049_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60739.1"
                     /translation="MSKDKIAQNKIETITQYTHRLQNNKVDSKGYKSVETTYDHNGNI
                     VEVVNYKPNGVVASKHLYKYDDRGRKIEYQQFQELPKKGFALAFRQAFTYDSKGNKLT
                     EIGYDGQTTYRVAYSYLPDGKQKDITKYSANNAVEEKWVFEYSTSKVKISIYKPAETL
                     NKIIERTLDSQGRVIDEKNLTPAGKELGRTAFKYGDGDMLLTKVEYYGGELRATYDYV
                     YDEKKQMVEVYMTKPGTSKILYSSYKYDKNGNLLEEKWYEDGASDYSRRNYKVDNQGN
                     INEVDAYYSDYKYRVVYRYSYKFSR"
     gene            37900..41235
                     /locus_tag="PK049_03930"
     CDS             37900..41235
                     /locus_tag="PK049_03930"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOK60740.1"
                     /translation="MRHYLKLFLFFALAITSISLFAQEIGRYPLVNFHHRQYNGHSQS
                     WAITQDNRGMIYVANNVGVIEYDGSEWKHISINNALARCLDTDPTGRVWVGGQDEVGY
                     LAADSANSLKYYSLSKLLPERFAKPGLIRQIYHTGQAVYFSTFKALFRINSDLTVNYW
                     EPKTQFHRSYYVDNFIFINQRDYGLTYLYNDSLVKVPGTEILGTQMVYTILPFDYQYL
                     LVGTQTNGFYLLNKQALTTRTLPTDFKTLIPFKTSNDNFFITNRIYSGIMLDEGLWAI
                     GTYSGGVAIINHSGKIVRTISKETGLQDNTAWYLYVDNSKNIWMALNNGISYSPFHSQ
                     LTSWTEQDGISGVIQSAVNFKGTIYYSSNTGVHVKTNSHFERVSGINDLAWGLFIYTD
                     ESGKEHLLSGTANGIYRIEGERAIPVTSIKVNAYSFIKSRIYPGILYVGLYDGVGVLQ
                     ENRGVFTFLGYLYSSSSEVYKVTEDKNGDVWFTQRYKGIGFLDVINPYELIAEKYKSY
                     SLPFQPKCDDIAVNYIDGLVLASTEGGLSSYDKDTDSFVPETMLGTQYANGTIGLRIL
                     ACDRNNDIWFETYRFQANRKLERVTKHADKAYRRSPAQFNLIPETIFFDVFTDSDNVS
                     WISSTDGLFRFDANEHFPTDQIPRVFIRQVYSKKKGTVYGGFSFDSLATQPFVRAKSV
                     QVQQPIVLPARVNDLIIFFSSPYFQPNHQLQYSYKLEGYDDDWSPWNTSRFKEYTNLK
                     PGSYRFMVKSKTLQDIESPVEYFPFIIDHPFYLKWYAFILYLALAGLIILVIVRINTR
                     ILRVSNIKLQQLVDERTKELKESERALTEKNLQLQHQKEEILSQRDELEERNRHINDS
                     IQYAKTIQQAILPDLDTVFANDFEHFLIYLPKEMVSGDFYWVAQAGPKGKPNDKLFVA
                     VVDCTGHGVPGAFMSLIGSRLLSEIVVERKNHNPAAILTELNDEVNKALRQDVSESFD
                     GMDVALCLIERKSDNAYVVTFAGANRPLYYCLKGDNNMQTLKGNRKSIGSVLPDVEQE
                     FSNWRISMHAGDILVMATDGITDQNNVLRKKYTTCRLHTSIVSALGKPMPQMQSIILD
                     DFLNFKADEPQRDDITVLGIRFK"
BASE COUNT        11275 a         8921 c         8990 g        11921 t
ORIGIN      
        1 gcttggctaa ccacaaatct cattattact ttaatactgc gtcattagtc attattcaaa
       61 tttttttcta ctaccgatta aaacacctat tgcaatccca aatgccattc cgcttcctat
      121 tccaattgat aaccaaagtg cgatgtcgtg cagcaacact cctatagtaa tcccagctat
      181 tgctcctaag acaagaccaa ttgaaagccc tattgccatt ttattattct tcttgttgtc
      241 tattttttta ttgattcaat tgcaagttta acataatctg gggattatat attggtttac
      301 ctgtgccagg attttgctcg ttcaacggca tcggcccatt tatccttcat tttttgtagg
      361 gtttcggctg gcataccggg agtgaaagtt ttatcgagtt gccaaaggtt ggcaatctcc
      421 tcaatatttt tccagtaacc cgtggctaac cctgccaggt aagctgcacc catagcggtt
      481 gtctctattg ttttaggccg tacaacgttg atgccaagca catcggattg gaactgcatg
      541 agcagattat tagctgaggc gccgccatca actcgcaatt cagtaaggtt tatgccggta
      601 tccttagcca taacatcaag cacatccatg gtttgaaagg cgatgctttc cagtgcagct
      661 ctggcaatgt gtgcacgtgt tgttccgcga gagataccaa tcatcaaccc gcgtgcatac
      721 tgatcccaat gcggtgcacc cattccggta aagcttggta caaggtataa atcgccattg
      781 tcgggcacct ggcgggctaa ctcctccacc tcagcgctgc ttttgatgat acctaaacca
      841 tcgcgtagcc attgcactac agctccgcca ataaaaatac taccttcaag ggcatagtag
      901 gtgtcgccat tcacttgcca ggcaatagtg gttaccagat tattttttga gagaaaaggt
      961 tttttcccgg tgttcatgag tataaagcaa ccggttccgt aggtgttttt aaccatgccc
     1021 tggctggtac acatttgtcc aaaaagggct gcctgctggt cgccggccat gcctgcaata
     1081 ggtacttttg tagcaaaaaa tgtagtattg gtatggtcgt aaacttcgct cgacgaggcc
     1141 acacgaggca aaatgctctc aggtatttgt aaaagatcga gcagctcttt gtcccatgtt
     1201 agggtgttaa tgttgaaaag catggttcgg ctggcgttgg aaacatcagt tacgtgggtt
     1261 cttccgcggg ttaacttcca cactaaccag gtgtccactg taccaaaggc taactcgccc
     1321 cgatttgcct tttcgcgtgc accgggaaca ttatcgagta tccacttaac ctttgttccg
     1381 ctaaagtagg catcaatcac cagtccggtt ttttgtcgta tcgtgtcagt gaatcccttt
     1441 gccctaagct catcgcaaag cgatgaagtt ctgcgatctt gccatacaat ggcgttatag
     1501 atgggttcac cggtactcct gtcccaaaca atagtggttt cgcgttggtt ggtaatgcct
     1561 atgccatgca gattttgacc gttaatccca atcttcgaca tggcttcggc tgcaaccgcc
     1621 acctgcgatg accaaatctc gttggggtcg tgctcaaccc agcccggttt agggaaaatc
     1681 tgctgaaatt ccttttgggc catggaacga atgttcccct tttggtcaaa aatcagggcg
     1741 cgggaactgg ttgtgccctg atcgagtgcc attataaact tttccatagg tttaggtcga
     1801 ttttaggttt ttttagggtt tcacaaatta gctaagaatg tagttctgtg caatagctag
     1861 gtaattttcc acctgttcgc gttcccagtt tttatcacgg ttcaattccc ttgccattat
     1921 agctgctacc gcaggtgcaa tgcgcatact ctcgcgagca tcgaggagca ggcatcgggt
     1981 acgacgcgcc aaaaagtctt ccacattacg agccatttca ttacgaactg cccacaccac
     2041 ttgggcttta ataatgccaa gactctggct tagggtttcg ccaagctcag gctctactct
     2101 tgccagttca aggatctttt ccttgtcggt tccatacaca tagagcgggt cgctatggtt
     2161 aacgttgttt gtgtaaccat gaattttcag gttccgggtt tttgttttac ttttctccca
     2221 acctttaacc gattcaaccc tattcaccat atcctcagcc atcctgcgga aggttgtcca
     2281 cttgcctccc accatggtaa agagctcgga ctgggttaca atgattttat ggctccggga
     2341 aatttcctta gtctttttcg attcccctgt gggtgccgca agcggtcgta atccagcaaa
     2401 cacgcttagc acatcgcttc gtttgggtgc tttggttagg tatcggccgg cagtgttcag
     2461 aataaattct atttcctcct caagcgcaac aggttcaagc gatgcgttag gtatcggagt
     2521 atcggtagta ccaaccacaa tgcggttgtg ccacggaaca gcaaatagta cccgtccatc
     2581 gtcggttttg ggtatcatca tggcatggtt gccgggtaaa aagcttttgt cgagcacaac
     2641 atgaacaccc tgacttggcg atatggtttt tgcctggccg gggatgtcca tttgcagtac
     2701 gtcgtcagca aaaacgccgg ttgcgtttat tattgctttt ccatggaaag tgtagctcct
     2761 gcccgtttcc aaatcctcag ccacaacccc gtcgacaata ccgttggtgt ttttggtgag
     2821 tttaaccacg ttcatgtagt taagcaccat ggcaccatgt tcggttgctg tttgaagaac
     2881 atttatggcc aggcgcgaat cgtcgaattg accatcgtgg taaaccacac cggcagtaat
     2941 ctttttaggt tcgatggttg gaatgtactc cagcgctctt ttctttgaaa ccctgagcga
     3001 acgcccaagg ctgtagctgc ctgccagtaa atcgtaaaac gttagaccaa cggtgtacat
     3061 cagctcatcg aaccagccaa aggttggaat cacaaagctt tgatttttta ctaagtgggg
     3121 agcatttttt agtaaccttc ctcgctcgat acaggcctcg cgcaccaaag caatatcgcc
     3181 ttgagccaaa taccgcaccc cgccatgtac cagcttggta cttttactcg aggttgattt
     3241 ggtgaaatca tgtttttcca gtagcagtgt tctatagcca cgggttgctg cttcaagagc
     3301 aattcctata cctgttgcac cacccccaat aattacaaag tcaaatccct gatcatttga
     3361 cacagcttgg ctgagcatct gttctctatt catatcgtaa gaattagtaa tacaacgaaa
     3421 tatttttggt aaaaataaca aataaaacga aacaaaacga aattaaatga aattattttt
     3481 taggtatcta cttaaaacat cggatttaaa aaatcactat gtttgagata aaattggtag
     3541 gtatgacatt gagcatagcg gaacggcata gaaaaattct tgaaaaactc aaggagacag
     3601 gctacgtatc ggtagttgag cttagcgagc aactgggagt ttcagcggtt accattcgaa
     3661 aggatctgaa acttcttgaa agtaggaatc tgcttttccg ctcgcatggg agcgcatcgg
     3721 taaataaccc gtacatagcc gataggcatg taatagagaa agagcagcta tatgttgagg
     3781 agaagcagcg catatcgcgt tatgcggcca gtttagtttc cgatggagag agtattattc
     3841 ttgcctcagg gagtaccatc aatgagtttt ctcgccaaat aggcgaacga cgtaacatta
     3901 ctgtcatatg cgcctcgctg gttgccgccc gtgagcttgc cgtaagggga gtggctgagg
     3961 ttttacagct tggaggcttg gtaaggcata ccgccacatc ggttgttggc ccctttgccg
     4021 aaaagatgct cgagggcttc cggtgcagca agctctttat gggtgttgat ggtattgacc
     4081 ccgaatttgg tctaaccaca accaatgccc ttgaggcctc gctgaacagg caaatgatag
     4141 ccagcgccga aaaggtaatt gttttggccg atagctcaaa attcggccgt aggggtttta
     4201 gtcggatatg ccagctcgag caggttgacc tgatagttac cgataaaggg gttaacgagc
     4261 tgtttgttaa acagcttgag gaaaaaggtg tagaggttgt gttggtgtaa gggctggcta
     4321 aacggtagca ataaccgtaa ggtttccggt caccttctgg ccaatggcta ctttaatgtc
     4381 ggcgttaagc gggaggaata catcaacccg tgagccaaac ttgataaaac ctagctcaga
     4441 gcactggttt acaggtttcc ccacctcggc gtagcaaaca atccttcggg ctactgcgcc
     4501 agcaatctga cggagtaata ctttaacacc atcgggacgc tcaaccacaa ccgtggttcg
     4561 ctcgttatgc gacgacgatt ttgggtgcca tgcaacaagg taatcgccag gatgatactt
     4621 gtagtacttt ataacgccac ttactgggaa ccagttgata tggacattcc aaaccgacat
     4681 gaatattgaa acctgtatgc atcgggtttt aaggtactcg ttctcttcta cctcctcaat
     4741 tgtaacaact gtgccatcgg caggcgacaa aaccgcattc aggcgggttt caatgtgcct
     4801 tgaaggtact ctgaaaaatc gggttaagaa agccattagc agcaccgatg ctaccgttgc
     4861 aacaattgaa attgcttttg gcaggattaa ccaggaaacg gcgcatagta ccaccaggac
     4921 tgccagtgtt acaaaaagaa ttgagtatcc ctccttgtga accttcatat ctactaagaa
     4981 atttaaataa tcatcattac ttcaaggtaa acataaacca tggggagtac cattaacaat
     5041 gcgtcaaaac ggtcgagtag tcccccgtgt ccaggaagaa atttcccgga atccttaacc
     5101 cctatactgc gtttgaacat cgattccact aaatcgccca gtaccgccat aaaggttgta
     5161 attagtccta ttgcaagcca gtgcttaagg cttacctcat cgaaaaacat tgcaataacc
     5221 catgctacca gtagcgtggt ggctataccc ccaacaaaac cttcccagct ctttttgggc
     5281 gatatccggg gaatcatttt atgcttaccc atactcacac caacaatgta cgctccggta
     5341 tcgtttgccc aaaccagaaa gagtattcct agtaatatgt tcgggttgta atccatgcta
     5401 taggaggagc cattgatggt aatgaagttc attaacgaaa acggtaaggc aatgtagaaa
     5461 attccaagca gggttaatgc tatgttctgc attggctttt gctggtgcct gtaaagttca
     5521 acaacaaaaa ttgaaacgag caaaggtaca aaaccgtaaa gcgttagctg ctcaatccgg
     5581 cctgagctgt agaagtacga ccaaataaag aaggccatcc caatgaatac tccaagtaac
     5641 gattgtggcc gtaacttggc tttaagtcct aaatggtaga actcaagcat ggtggcagtg
     5701 gtaatggcta aaaaaagaat gaaaaaggtg atgtggccaa tgtaaattgc cccaaccagt
     5761 acgattacaa aaagtaatcc gctaatggta cgttttaaaa actcgctcaa ccctgtatat
     5821 cttttaaaag ttctttagcc ctttcagcgt tttcaggctc tgtatacact gcaacttcgc
     5881 caaaaagata gctggaatct ttacggttaa ccacaacagc aggaatctcg ttttcctcga
     5941 ggatttgttt agccatttcg gcctgccatg tatgcgaggt ggaaaacacc tcttgccagt
     6001 tttggtccat ggtttattcg gtttggtttt ctttgctttc tgaattttgc ttgtgagcct
     6061 cctcgtcaag ctcttttacc agttcttgct cacggcttag ggttttccgt gggccaaata
     6121 tcttttccaa atcctcgctg aagataactt cacgttcaag tagtagctca gcaagctgaa
     6181 catgaccttc gtggttctcg gtaagtatct gcttagctct ttcgtaggct ttatcgatta
     6241 tttctttaac ttcctcgtca atttgttcag cggttttctc gctgtagggt ttggaaaatg
     6301 tgaattcaac ctggccggtg gaatcgtagt agcttatatt tcgaagattc ttgcccattc
     6361 caaagtaagc aaccatggcg taggcctgtt tggttaccct ttctaggtcg ctcagagcgc
     6421 cagttgatat cttattgaag tttatctcct cagctgccct acctccaagg gttgagcaca
     6481 tctcgtcgaa tagttgctcg gttgtagtga tttgacgctc ctcaggcaaa taccatgctg
     6541 cacctaaaga cctgccacgg ggtataattg aaaccttgag caatggatta gcatgctcaa
     6601 gcagccagct aacggtggcg tgcccagctt catggtaggc aatggtgcga cgctcctcct
     6661 ttgatataat cttattcttc ttttcaagcc ctccaatgat tcggtcgata gcatcaagga
     6721 aatcttgttt ctcaacatac tttttattct tacgcgcggc aataagagca gcctcgttgc
     6781 acacattggc tatatcggca cctgaaaatc ctggggtttg ctttgccagg aaactgatat
     6841 cgagattctc ctcaagctta agagggcgaa ggtgaacctt aaagatatcg agacgctcct
     6901 tgaggtcggg caactcaaca tgaatttgcc tgtcgaaacg accagcacgc aaaagagcac
     6961 ggtctaaaat atcggcgcgg ttggttgctg ccagaatgat tacaccctgg ttggaggcaa
     7021 atccatccat ctcggtgaga agttggttaa gggtgttctc acgctcatcg ttaccggaga
     7081 agctcgggtt tttaccgcga gctcggccaa tggcatcaat ctcgtcgata aatacaatgc
     7141 atggagcctt ttccttggcc tgacggaaca ggtcgcgaac gcgcgatgct cctacgccta
     7201 caaacatctc cacaaactca gaaccggaca tgctaaagaa gggaacgttg gcctcgccgg
     7261 caacagcctt ggcaagtagt gttttaccgg ttcccggagg gcctacgagt aaagcaccct
     7321 tgggtatttt accacccagc tcagtgtact tcttggggtt tttgaggaaa tcaactattt
     7381 ccattacctc aattttagct tcctcaagcc ctgccacatc cttaaagtca acccttgggc
     7441 gtgtttcctt atcaaacagc tgtgcctttg acttacctac cgagaaaata ttcccgcctc
     7501 ctggtccgcc tccaccgcct ctggaaattc tgcggaaaat gtagatccaa atggcagcaa
     7561 gtattacgaa cggaagtacc catgaaagta tatcggccca atagtttggc cggtcaacgt
     7621 attccgggta tattttttga ctttcctcta tattggtttg tgcctcggca agctgccgct
     7681 caaaggtttc aatggaacca atggtaaagt aaaagtgagg ccctgccttt ggaacagcgc
     7741 caaacccctg tcctagccga tccttgtact tctctagctt atcttcctta aggtaaacct
     7801 ctaccttgcc agcgttcctt accaccaata gcttgcttat ttcctgcttt tccagcattt
     7861 ggttttttac ctcctgccat gtggttagct gagctgtttt accagagtaa aaatactgga
     7921 tcaggaaaaa tccaagaatt ataatgatgt aaacccagta aacgttgaat cgtggcattt
     7981 tgggtttgcc gttttcatcg ttcatcatag ggttgttatt gttgttctca gccatatagg
     8041 actatttcta ttattactta ttattgttaa acttcttcgt actgtatagc tttagcatcg
     8101 gcccaaagtg cttcaacacg gtagtagttg cgccattcgg tttggaatac atgcactact
     8161 atatcgatat agtctagtac aatccaaatt gcattttggt aaccatcgga gcggtaaacc
     8221 ttttcgccca gcttctctaa cactttatct tcaatattgt ttgcaatggc atctacctgt
     8281 gtggtggagt cggcattaca gaaaacaaag tacttgcata cggcattcgg caaagctcca
     8341 atctcaagcg atacaacgtt ttttcccttt ttttcttgaa tagcatcaac tatacacgta
     8401 agtaaatcct cggtgttcga tgttttcttt actcttttta ctgccattta atccgttttt
     8461 actaaactgc aaaattaatc aaaaaaatga acatcaaccc gtttaattgt tttacataaa
     8521 caggtttaag ctcatggttg tagctaaata aagctatata aaaaaacgta aaggggttaa
     8581 cccctttacg tttgagcggg aaacgggact cgaacccgcg accctcagct tgggaagctg
     8641 atgttctacc agctgaacta ctcccgcctg tttacactgc aaatttataa catatttagg
     8701 caaattggtg agccaacacc ctaaaaaata tttaagggat caataattga cgatagtaaa
     8761 tcccttgttg tttgtgttga tgatgcattg ttaaggctga taaccgggac cttggcctca
     8821 ttgataatgt ggtgagcaaa tgcgcccacg tagttatcaa agcccacgtt ttctttatgg
     8881 gtcataatta taatggagtc gcacttaatt tcatcggcgt aatcaagtat ttcctgatat
     8941 gcaaaatctt catccttgcg aatcaatttt ttagtatggg gtatgtgatt ttcagcataa
     9001 acagccgcat acttttcaat cttttcattt attcggcttt tttcagggtc gatatcccca
     9061 aaaattaccg atacgatgtt tagtttagag ccgaatttta gagcaagggc aacagagtta
     9121 aataggttta cacggcaatt tttagtaaga tcaaggggaa caagcatatt cttaaagatt
     9181 gtttcaggaa cggtttttac ggttattaca ggcttggtag ccttcataat cacgttctcc
     9241 agggttgatc caattctttt tccttcctgg gcaaaggggt aattatcgac cataaggata
     9301 aagcggacgt tttcctgctc agcgtattcc acaattttat ggtgccgttt tccggtttgc
     9361 accatcagct ttgagtcctc aggtaatcca aactggcgcg atatgttaag cagtttatta
     9421 aaggcctcat tgataagccg ttcctcttta acctcatcga gccaccaatc ctttaaatca
     9481 agaacatgaa gtaaatggat ttgtgatttt gtaattgggg ctaatttctg agcgaattca
     9541 atggcctttt cgtagtagtc cccaaaatca accggaacaa gcagtatgtc gggcgattta
     9601 tcttttttag ttggcatttt agtccttaag tttagcggtg atatattcgc gatttagctt
     9661 tgcaattgtg ctaatggaaa taccctttgg gcattcagcc tcgcaagcac gtgtattagt
     9721 gcaactgcca aaacctagtt catccatttt ggctaccata cgcttaaccc gctcgggtgc
     9781 ttcaacccgc ccttgaggaa attgtgccag gtgggtgatt ttggcagcca caaagagcat
     9841 agcggatgca tttttacaag cagccacgca tgccccacaa ccaatgcacg atgccgtgtc
     9901 gaaggcaaca tcggcattgt gtttaggtac aggaatagca ttggcatcct gtgcggcacc
     9961 tgcgtttacg ctaatgtagc ctccagcttg tataatctta tcaaaggctg agcggtcaac
    10021 cactaggtct ttcacaacag ggaaagctgc tgcgcgccag ggctcaatgg taatggtatc
    10081 gccatcctta aagcggcgca tacgcagttc acaggtggtg gttcggtggt catttccatg
    10141 gggtcgacca ttgatgtaga tgccgcagga accgcaaatc ccctcgcgac agtcgtgatc
    10201 gaacgccact gggctttttc cctccctaac cagtcgctgg ttcagcgagt cgagcatttc
    10261 aaggaacgac atatcggggg caacattatc gagctcataa acctcgaatt ttcctttact
    10321 acgggaatct ttctgtcgcc atatcttgag tttcaccttc atagccttta gtgttatatg
    10381 gttgatgaga tgctatttat agctgcgttc ttgaagcttt acgtattcaa atttcagctc
    10441 ttccttatgg agtattggct ccttgccatc gccggtaaac tcccaggctg caacgtatga
    10501 gtagttctcg tcgtcgcgtt tggcctctcc tgtttcagtt tggctttcct cccggaaatg
    10561 tgccccgcaa ctttccttac ggttaagtgc atcgtaaacc ataagtttac cgagttcaag
    10621 aaagtcggct acgcgatttg ccttttccag ctcttggttc agctcgctgg cagtatcggg
    10681 aaccttaagg tttttccaaa attcatcctt tagctcatcg agcaatgtaa gtgccttttt
    10741 aagcccttcc tcgttgcgaa ccatcccgca gtaatcccac atgatctttc caagccgctt
    10801 gtggaagctg gttgccgttt cggttccgtt aattgccatt aacctttcaa tccgttcctt
    10861 aacctctttt tcggctgcat cgaactcagg ggtattagtg ggtatttttg gtgtttttat
    10921 ctcgtcggct aagtaatcgg caatggtgta gggtagtata aagtaaccat cgcccgaggt
    10981 ttgcatgagt gagctggcgc ctaagcggtt tgccccatga tcgctaaagt tggcttcgcc
    11041 aatggcgtat aggccaggga tggtagtcat caggttatag tcaacccata gccctcccat
    11101 ggtgtagtgg ccagcagggt aaatgcgcat gggtacctcg taaggcgatt ctcctgtaat
    11161 tttctcatac atttcaaata ggttgccata gttctcctca atgtacttac ggcctttttt
    11221 ctgaatggca tcgcggaagt cgaggtaaac cgataggcct gttgggccaa ccccgtaacc
    11281 ggcatcgcag cgttccttgg cggcacgcga agccacatcg cgcggaacaa ggttcccaaa
    11341 agcagggtaa cgacgctcca ggtagtagtc ccgttcgtcc tctggtatct cgttgggttt
    11401 gcgggggtca cccttagttt ttggcaccca cacccggccg tcgtttctta acgattccga
    11461 cataagggta agcttgcatt ggtggtcgtt tagcaccgga atgctggttg ggtgaatttg
    11521 cacaaaactt ggattggcaa acagtgcgcc acgtttatgg gcacgccatg cggctgagcc
    11581 atttgagttt agtgcgagtg ttgagaggta atagattgtg ccgtaacctc cagtggctaa
    11641 aactactgca tgagccgagt ggcgctcaat ctcgccggtt actaagttgc gaactataat
    11701 ccctcgtgct tttccgtcaa ttactacaag atccatcatc tccctgcggg ggtacatagt
    11761 aacagttcct ttatcaattt ggcgcgagag ggcggagtag gcgccaataa ggcactgttg
    11821 gccggtttgt ccctttgcgt aaaaggttct tgaaacctga acacccccaa acgaacggtt
    11881 ctcaattaat ccaccatagt cacgggcaaa aggtactccc agcgcggtgt agtggtcaat
    11941 tacaaggtta cttacctcag cagcacggta aacatttgct tcacgggctc tgaaatcgcc
    12001 acccttaatc atatcgtaga acagcctgta aacgctatcg ttgtcgttct tatagttttt
    12061 ggcagcgttt attccgcctt gtgcggcaac ggagtgagcc cttcgggggc tatcctggta
    12121 gcagaacact tttacgttgt accccagttc gccaagtgtt gatgctgccg atgaacctgc
    12181 aaggcctgtt cctaccacaa taatgtcgag ttttcgtcgg ttggctgggt ttaccagtcg
    12241 gagtttcgct ttatgattag tccatttttc ggccaatggg ccttcaggta ttttggcgtt
    12301 tagcttactc atagctgtcg atatttataa tcggattaaa atctacctaa acagtaaaaa
    12361 atagattggg ataatgctaa atcccgttcc aataataatg gcgtaaacat aggctaccac
    12421 ctcaaggcgt tttaaccatt ttgtaccatt ccatccaagg gtttggaaag cagcccaaaa
    12481 accatggctc aggtgtaatc caagaaaaat tgcacctatt acgtaaagca aatcatacca
    12541 ccagtagctt atgaaaagac ccgaaaccaa tgcgtatgcg ttgggcatag tgtcgctccc
    12601 gtaagtaatg gtttcaacgg ttccaaactt tagtttccaa aagaaatttg ctaggtgtat
    12661 gattaagaaa atgaaaatgg tagtacccag aatgaacata tttttcgacg accaggttga
    12721 aagaccatta gatgtagttt ttgagtaacc tattggcctt gttctttgat ttttaatggt
    12781 caggaaggtt gcatagataa tgtgaaacac aaagccgatt gccagcaagg gctcaattac
    12841 cttaatggca gggttgctgg ccatgaaatg agctgctcgg ttaaaaacct caccatcgcc
    12901 caccagtagc agcatgttta cagttaaatg aactagcagg aataccatta gaaacaatcc
    12961 ggttatgctc attaccagct ttttcccaat cgatgagtta aacatttcaa tcatagttta
    13021 tcggtttaat tggcagtaca agttattaaa ttttgctttt catctaaagc atatgaacta
    13081 atttaacatt ctaaataact tttccaacac gcattttatg taccgacaac acataaggcc
    13141 ttgataatga ataaaaataa agggtttctc ttactgagaa accctgtaat attcaatatt
    13201 tcaaaggatt aacaaatcct ttgtgggcaa tacaagatag gatagcctag ttgttaacta
    13261 ctgtactacg gtaaatttta ctgtaggctg ggcaagtttt tttggagcat gaacttgcta
    13321 atactgctac aaatgcaagt agcaataatc cagttacgat tcttctaatt ttcataggcc
    13381 aaaattttat acaaatatac tttactttac cacacttgcg taggccggac atggaatatt
    13441 attatggcat gaacttaaac ctacggatat aataatacca acaactatta ggactaaaag
    13501 tttaccttta agtttaaaca aatccttcat cgcttctaaa acttcaatta ttataaccta
    13561 tttataaact tccgcaagga ataatttttt tgcaagttag taaaattatt ttgacaaaac
    13621 gaagataatt gatgatttgc attaccattt cattgataaa aacacaaaat gttgtaagca
    13681 agttttttgc cgtttcggtt tgctccataa agatattaac agttaggctt aagccttagc
    13741 atgaacaact attttatata actttccaga gtgaaaaagt tgttataatg acaagccaac
    13801 tttatgctga tgttattttg cctttagctc ttcaggcaac atacacctac tctgtaccct
    13861 ttgagatgga aaaaatgatt caacccgggg tacgggttgt tgtatcgttt ggccaacgca
    13921 ggctttactc agctctggtt tacagggtgc actctgaaaa accccaagtg caacaggtaa
    13981 aggatatttt acaggttatt gatactacac ctattgtgct tcccagccag tatgagctat
    14041 gggaatggct tgctgaatac tacatgtgtt ccctgggcga ggtaatgaaa gctgcgcttc
    14101 catcgggatt aaaaatggag agcgatacct acattgcttc tggcgacttt gatgaaattg
    14161 acctctccga tgaggagatg acccttatcc atctgctagg gcaaaagcgt aagcttacca
    14221 tccagcaact tacccaaaaa tcgggtctca aacaccctat gagcgttata aaatcgatgt
    14281 tggagcgcaa attgcttttc ataactgagg atattgagtc cgattatcgc cctaaaaccg
    14341 aaaattatat tgctttggca ccggaatact ccgatgagaa aaagctctgt gagcttattg
    14401 attcacttaa acgtgcaccg gcacagcaaa ggctaatact tgctattgcc tcgcaggcaa
    14461 ctactccaaa cgatttatct actgcaaggg ttctcagaaa gaccctgctg gaaaaggttg
    14521 atgtgtcggc atccgtttta aaatcgttaa ttgacaaagg atttatagtc caggatgagg
    14581 ttgaaatctc aaggattgat aatgctaata gcgatcctcc tactcgtaac ccccttgccc
    14641 tggaagattt tcagattaac gccctcaacg aaatacaaaa ccaatttagc tcacacgatg
    14701 ttgtgctgct acacggagtt acctcaagcg gtaaaactga gctctacatt caccttatta
    14761 accaagcact acaaaatggg aaacaggtgc tttacctgtt acccgaaatc gcattgacca
    14821 cacaaataat taatcgcctg cgcaaagttt ttggggatag ggttggtgtt taccattcca
    14881 agttctcaga ctctcagcgc gttgaggttt acctgaaaat gctaagcgag gagcctttcg
    14941 atgtaattct cggggttcga tcttcaattt ttttgccttt ttcaaattta gggttagtaa
    15001 ttgttgatga ggagcatgaa aataccttca agcagtacaa ccctgcaccc cgataccacg
    15061 ctcgtgatac tgcaattgtc ctagccaaac aggttggggc aaaggttttg cttggtaccg
    15121 caacaccggc agttgagact tactttaacg ctaaatccgg taagtttgga ttagtaaaga
    15181 tgctggagcg ctttagtggc ttagaattgc cacgtactat tgtggttgat acccgcgatg
    15241 ctcgtaagcg aaagcgaatg aagtcgcact tttccgatac tctcctgagt gccattgaga
    15301 acgctctttc ccaaaaggag caggtaatac ttttccagaa caggcgaggg tatgctccat
    15361 ttattgagtg tgccgattgc ggttgggtgc cccactgtga gcattgcgat gttacgctta
    15421 cctaccataa gtataccaat cagctaacat gtcattactg cggctttggc atggagcaga
    15481 tgggttcatg ccttgcatgc gggagtacaa acctgcagcc taagggtttt ggaaccgaaa
    15541 aggttgagga tgaactttcg gttttcttcc ccgatgccgt tattgagcgt atcgatttgg
    15601 attctacccg ttcacgctca tcgtatgagc gaattatttc gggctttgag caaggtaaaa
    15661 ttgatattct ggttggcacc caaatggttt ccaaggggct cgattttgac cgggttagtc
    15721 ttgtaggcat tctaaatgcc gataatatgc ttaatttccc cgatttcaga gcatttgagc
    15781 ggagttttca gctgattact caggttagcg gtagggctgg gcgaaagggg aagcagggtt
    15841 tagttattat tcaaacagcg cagcccgatc atcccgttat taaaagcgtt acaggtaaca
    15901 acttttttac tttctgggaa tggcagcttg cagagcgtaa ccgattccac tatccgcctt
    15961 tttaccgcct tgttcggatt accctcaaac ataaggataa gcaccacttg gaagataccg
    16021 ctgagaatct ggcaaaaacc ctaaggcatt cgctgggtga gaatgtactg gggcctgagc
    16081 ctccaataat ttcgagagta caagggctat atataatcga gattcttgta aagattaagc
    16141 gtgaacagcc cttgcagcga atcaaggaat ttattaggta tagtattacg atgttaaagc
    16201 aaaaaaggga aaccagcacg gtggttatcg ccattgatgt tgacccatac taaaccctaa
    16261 aggtaatcgg cttttgattc cttgtttgct gtaaaaggtg caatggcacg ctggtaaata
    16321 ccctgaacat aggcaatagc cattccgtag ttggtaacag gtatgcctgc ctctacggcc
    16381 ggacgcaagc ggttaataag ttgcttgcgg gtaatcatac agccaccaca ctgaataaca
    16441 agtgagtatt ccattatatc gcggggtagg gcatcgaggc ctgcaaccac atcgtactcg
    16501 agctgcttcc cggtaaagtt cgatatccac cttggaatct taactctacc aatatcgtcg
    16561 catgaaacat ggtggttgca actttcaagc agaagcaccc tatcgccatc ctttagctcc
    16621 gatatttttg gagtgccctt aaggtaattt tcaaaatcgc ccttcaggcg ggctaagagt
    16681 atgctaaagc tggttagcgg aatgtcgcgg gggattgatg catcggcttt aacaaaaatt
    16741 tggctatcgg taatggctaa tgcaggttta acctgggttt tacgtaggaa ggcatcaacc
    16801 tcacgttcct taagaacaac tgccacggca tcgttgtcca gaacgtcgcg tatggcctga
    16861 acctgcggga gtatcaacct gcctgcggga gcttcaacat caataggggt gataagtaaa
    16921 acaatatcac cgtacgatat aaggtcgcca agcagcgtag gtgtcctata tgacgattcg
    16981 ggaatggctt tcttgattag actaataagc tcgtcgggtt gggtttggga tttgcagctg
    17041 atatcaataa atcccgaatg cctcttgcca ctcagctttt ccttaaaaat actggaggca
    17101 ggctcaatgt cgctcttgtt atgaaccact aggtatggtg tatcggtttt gataaactcg
    17161 tttaccagca tctcctcgta gtcgccccaa gtatttgagg taacaaccaa cagggcaagg
    17221 tcaatatggt taatggattg tagggttttt tcgacccttt tctgtccaag ctcacccaca
    17281 tcgtcaattc cggcagtatc aaccagtatt acaggtccaa atccggttat ctcaaacgac
    17341 ttttttaccg ggtcggttgt tgttcctgca aagtccgata ctatggctac attttgccca
    17401 gcaagcaggt taattaacga gctcttgccg ttgtttctgc gtccataaat cccaatatgc
    17461 ggctttgttt ctttcccttt ctgcataaaa catgttgtta cttcacaaaa gtattgaaat
    17521 atttaacaaa aaataacctg atagtcagca caatgttcaa atcatttggt taacttgcat
    17581 tactttaaac atataaatta aaaaaaatgg tacgaaaaac atttgcattt gccttggtta
    17641 ttgccctaat gggagtaacc atggtaaccg aggctcaagt aggtggtttc ctgaaggata
    17701 aagcccaaaa agccgttgct aaaagtttga aacgtggcga ggatgaaaaa aaggttgaag
    17761 aacagcagca ggaagaacaa ccagaaaaac aggggtataa gcaaccaagc gctgccgata
    17821 ggtatatgca acaaaagatg atggggctta tgggtttcaa aaacgtgaag tttgaacccg
    17881 cctatagctt tacatcaagc atgaagatgg atgttgaatc tgtcgattca gcatcaacag
    17941 aggtaaacaa gggcgcatac gccttctact tcgacaagaa ctcacaaaac tttgccatgg
    18001 agtttgaggc attgaatcgc gaaacgggga agaaggaaaa atcattgatg atttttgatt
    18061 ataagaacaa agctatgctc attctaagcg ataaagatgg agagaaaagt ggtatagcca
    18121 tgaactttga acccgattca gtccaacagc aacaggggaa ggatgagctc gccgagcctg
    18181 tacctcaaga agatttatcg gcctataata tgtactacaa ggcaacagga cgcacaaaag
    18241 atgtactggg ctataaatgt aaggaatacg tttatgaaaa cccagatggt aaggtagagc
    18301 tttgggctac aaatgatata aagtatgact actccgaggc ttttggacac atgaatggtt
    18361 tccagtacct agctactgga ggaacagctt acttattagg tacggtgctt gaaatgcatt
    18421 tcagggatgc taactcaaat gcccgtgccg atttctggat aaaggagttt aatccaaaca
    18481 gatccagaac acttagtgtt gccgattacc aagttgttgg cttaggtgac aaaaagaagt
    18541 agattgaata aaaaccgttg taccgtgaat cagcaaatta aactggccct atattaggtg
    18601 cggatacata ttaagaggaa tttgtcagta aaatctagtt attaaatgtt tatggtgttt
    18661 ctattgccta agctgggttt aatatagttc attactttca ataattttat gaaaaaaaga
    18721 ggctggggta taaaaccagc ctctttccat ttaaaaccaa gtaggttttt gttaatatat
    18781 atgttggtta ggtttgggtt actgtgcaaa gaagtattcg tttaagtcgt acaaatcctg
    18841 tagggttagg ttgtttgtcc cgtcaaaaaa ctcatttacc gctcgcatca gttcctggaa
    18901 cgagatgtat ccatcggaat cggagtcaac tcccttcagc ttttcaggaa tcatacccgg
    18961 tgtatagttt aaactgcgtg accaaacatg ggttacaggg attaagcgaa cctccttgcg
    19021 cgaaacagct tcgctctttc ggttaaacat ggcagccaga acatcggctg ttacctgcga
    19081 accactctca tcaacaatag caccttccgg ggtattaggc tcctcatcca tgtaatcgaa
    19141 aacgccatca aagtccgtat cggatgggca tcctattgaa tcgacagcaa caccagccgg
    19201 ggtgtcgggg cactcgtcaa acctgtcgaa aaccccatct ccatctgcat cgctaaagaa
    19261 aatctcgtaa tcgtaatcct cacccagcat agcaaaaacc ctctccacaa gaattgtttt
    19321 tggatcggag aacaggtcga aattcatggt aaagtaggta aagctaaata tatccttaat
    19381 attattctta acaggatatc cctgggcttt ggctatttta ctgtcgtagt tgtcaatttt
    19441 atcgctaaag cagtaattaa aagcagctcc cacccttagg tttaccctat cggagagata
    19501 gaagtcgaac ccaaaatcaa ttggaactga gtaagtttgt tgcgagatac taccctgctt
    19561 atagagatct tttcctgaca agtctgtttc ataatcgctg tcaaatctta ctattcttgc
    19621 acggtaggca tcaggcccat tctcagggaa atttcgcata gttccatcgg accagaaata
    19681 gtaaaagtta cccgatccgt tgttgaggaa attgcccttt gggctaaaga aaatggttga
    19741 aaaacctact gataggtagg gtttaaagcg ctttgcaggt ccaaaaacat gaccaaagtt
    19801 gtactcaaac gataaaccta actggaaaat gttggtttta aaggctgagt ttacataaat
    19861 tggattgttc aaatcgtcaa gaggtaatgg ctgctgttgc ataaggtagg agagtttggg
    19921 atcgtgggca aaaatatcgc catatatggc aaagatattt cctttaaagt agtgcttgcg
    19981 gcccaccagg ttggaaacgt taagttttcc tgccacattc ccgtaaagtg gagaactgat
    20041 agggtttcgt acatcacccc agaaactcag gtaaccaaca cccaacgaaa caaccggttt
    20101 gtaaaccgga ttttgaactt caactatggt atcgagtatt cctttgtagt attcttcttc
    20161 atcaatttga gcatgaagcg catttggaag caccactaaa agtgccaaaa cggttactaa
    20221 cctccctaag tgtaatttaa ttagttctct cataaaacta cctgtaccac ttttgactag
    20281 taatcattaa cgcttcctgc acggtaattc gagtcccgtt gttgtatgca gtaacaaagg
    20341 catcgttaat tcttgtggaa ttccaaataa gaattctaaa atcgcgagcc tccttgtact
    20401 ccttgaaaga accgatagaa tacttatacc atccctcatg tttttcggtt ctaacatcat
    20461 atttgatgtt atagcgggta aagtatcgtt taatatcaat gggtttatgt ccggctgcaa
    20521 tttgaaccct atagtaaacc cctttttcgg gcataagcat aaagggttcc atatcaaatt
    20581 ccggcttggc tttacgtttt ggcttgtctt caattttttt atactcaatt ggaatgtcaa
    20641 ttccaccttc ggagtaaggg gtaaaacctg agatgatatt tgctccttcg gttgagaaag
    20701 ttgaagaaat tagtccgtca agcgcagcag ggtcgatgtt cgttaggtcg atatcctttt
    20761 gaatgatatc tataacccta gtagcctctc ccacaaggta tgagaaggtt ccctttaagc
    20821 taacggtaat atttcctttc ccatcggggg caatgagttt gtacgataca acaaatcggg
    20881 gttcggccgg gaggttcatc caaaggaatt taaccttttg gtccttaaag gtgaaaacag
    20941 catccttggt ttcaatcgct tcggctgtaa atccttcagg aacgtcttct tcaattttgg
    21001 caaacttttg ggcattaccc ttgtttacta gcagttttac cagatagtca ttctcagcac
    21061 ctgaagggat aggggtttgc cttatgcatc taacattgct agccacaaat ccaactggcg
    21121 cttgtggtgg tataatggtt tgaaactcgt taatgtcaac aattaaattg gggtcaatcc
    21181 tggtcgatgg gttaatacta atggtggagg tttggaattc ggctgttttt cgttcattat
    21241 tgtcaacgta ggcaaatgta cctttaagcg tgaacgaccc tttaagcctt tcgtcaacct
    21301 ttacccggta aaccaaacgc atctcctttc cctcaggaag ccgaagccat acgaatcgga
    21361 gtttttgttc gtcaaatgaa aagtcgccaa gcgacgattc cactagctgg gcagttaaac
    21421 ccatgggtaa atcctgttgt aggcgagcaa aaccgctaag gtttcccttt ttcaagataa
    21481 cttcaacgtc aaactcgcta ccggcattta ctgccttcgg aacatttaca tttaatgcaa
    21541 catcgtttcc aatcaggaaa ctcagtaggg taatgccaac caggttatat aacactgtaa
    21601 cgatactagg catcgtttat tgggctaagg tttaggttca caatagggtt aattagttac
    21661 aatattaaga aataattgtt taaaatgtaa caaaaaagcc caaaaatcta atcttttggg
    21721 ctttcaaacg agtaattgtt actaacaaaa aatatgcaaa cagcaataaa tcagtacgat
    21781 acactgttga aaactttgtg ctatttatag gaaaaagtgc tacctcccag aaactcacgt
    21841 atgattgagg tgggtggtac ttcgcgttcc gatattggag tgaaatagct taagaactta
    21901 agcagccgtt gggcctcatc gtattctggc cgggttggcg gaacctcctg gattattggt
    21961 tcagcaaagc gttttaaaac gctaaactcg taaagcaagg ctgagttaaa cataacgttg
    22021 gcctcctcct ggaatgggaa gatgttttta tcctcgccac gacgaacact ttcccacctg
    22081 ccaatagtat ccagtgcgct atacccccga taccgatagt cccttactat tctgcgtatt
    22141 aaccgattat cggtggttgg tattcggttt atgccatcga ttgaaattga ggttaatgcc
    22201 gaaacgtaaa ccttgaactt ttggttatta gggattaagt gggttaatgc tgggtttagg
    22261 gcatgaatac cctcaattat tattacgcca ttgggttcaa tctgaagttt ggtgccatca
    22321 aaaaagcgct taccctcctc aaaggagaat tttggtattt ccacctcttc gcccttaagc
    22381 agcctgacca ggtcattgtt aaaataatca acatcaagcg cgtccagtgc ctcaaagtcg
    22441 tagtttccga actcatcctt aggggttttc tccctgtcaa caaaatagtt atcgagcgag
    22501 atggtgtatg gttttaaacc attaaccttt agctgaattg ctaaacgctt agcaaaagtt
    22561 gttttacctg atgatgatgg gccagaaata agtacaagcc ttaccggttc gggttgctgg
    22621 tgtattctat cggcaatgtc ggcaaccttt ttttcatgta atgcctctga aatttttatt
    22681 agttcactgg catttccttt ggctacctct tggttaatag ctcctacatt ctcaacgcct
    22741 aaaatctcaa gccaatcctt atattcctga aagatttcaa acattttgtt ctgaattact
    22801 acatcttcca cttcatcggg ctggaaccgt ttaggcaccc tcagcagcat tccatcgtaa
    22861 tacttagtga ggtcaaacac ttttaggtag cccgttgatg gaacaagact tccgaagaag
    22921 taatcggcca tgtcgccaag atagtaaact gaggtgtaaa gggtaggacg agtttcgaat
    22981 aacctggctt tgtcctcgta acccaaatct ctaaagagtt tgatagcatc ggatgttaat
    23041 accttatcgc gccgaaatgg caaatctgct gccacaatct cgcgcattct atcgcatatg
    23101 ttgaatataa gcggtaattc caggggcagg ttgcagccgt caatctcgca gtagaagcct
    23161 ttggatacgg aatgctcaat tcgtaccttg gcaccgggaa taatatcgcg tacagctttt
    23221 tgtagcagaa aaaatagcga tcgttggtac attcgcattc ctgcagggtg agtaatatcg
    23281 atgtatcgaa ctgtttttgg tgtaaaaatt tcgtagtcta actccttgag ctggttgtta
    23341 accatggctc ccagaatctg gtaatcaaat tttatttcga atatttttga gatttccaga
    23401 agtgagtgtc cgggagttac acgaaccttt tgcccggtat tctcaatcac tatttctact
    23461 tccttacgca taagtatcaa aattttctca aaaaatacaa aactttacag ctttatattg
    23521 gcaaagtaaa catcctcaat cttatcattt agtttaaggc ttgagccaac taggtttttt
    23581 agggtattgg tattactgct cgagtaaaac tcaatgttga tgctatgaac aaatggctta
    23641 ttacaaagta acccatgatt ttcagctacc cttttggttt gcaaagcaac cgctggagca
    23701 gggtccacaa tggtgatgtg ttcaggcagc attctttcca gcactggttt aaggaatggg
    23761 taatgggtgc agcccaaaac caaatggtcg atgttacaat caaccattgg gtcgatgtat
    23821 ctgtgcagga gctgttctgc ctcgggggaa tctgcttttc ctgattcaac tagttctact
    23881 aatccttcgc ccacctggta gcatacgttt acatcggtgg catactttcg gctggtttca
    23941 atgtacagcc gtcctttaaa agttccggcg gtagccaaga ccccaatact tttggtttta
    24001 gtgttttgcg aagctggttt tattgcaggt tccattccta tgaaagggat gctgtatgaa
    24061 cttcgtaaat ggtcaattgc agcggcagtt gcggtattac aggcaacaac tataacctta
    24121 cagccttttg atagtaggaa ctgtgtgatc gtctccgata ggccgataat ttcctctgca
    24181 ggtttggagc cgtatgggca atttgctgaa tcggcaaagt agatgtactt ctcgttgggc
    24241 ataatcttaa ccagttctga aagaactgtt agaccacccg caccggagtc gaaaattcct
    24301 ataggattac aattgccgct catttgcttc ctaaataaaa taggggttaa acgaacaaag
    24361 ttcagtttat cccctaaatt taaaggtatt gtaggtttac ttggtaatgc ctaactcttt
    24421 cttaaccaac ggagtaatat cgatactctg ttccgagaag taaaggagaa ctcccgagct
    24481 ggtatcgaaa atgtaagtaa atccatttgc cttggcaact ttcttaatgg cttcgtcggc
    24541 tttatcaatt acaggtttca tggtttcaac ttggtagcgc tggtaatcct gctcagcaac
    24601 ctgctggtat tcctgagccc tctgctgcat atcggtgagt tccttttctt tcatctcacg
    24661 tattgcatcg gtgaaggtgg cacgtttttg caggtaatcc tgatatttct tgttgaactc
    24721 tacctgaagt tgctcaatct gatcctctaa cgattcggcg tattttctaa ttttagcttc
    24781 ggcgctatcc ctgtcgggca tagctttaag tagttcctgg ctgttaatat gaccaaattt
    24841 atagttttgg gcgtttactc ctgaaaaact aaacgttagt agagcaacta ttgctaatcc
    24901 aagaatcttt ctcattgttt atcgttttta gtttaagcaa atatataaaa attatttaaa
    24961 acctaagcgc tggagaactt cttcgctctt gtcgaatcgt gggttggtgt aaatcatgtt
    25021 aggtgaagcg gcagcgtcaa atattacggc atacccccct tcaactgcaa tctctttgat
    25081 tgcgttaaaa acctgatcct gtatgggttt aaccagttcc tcccgttttt tgaaaagtaa
    25141 cccgtcgcgg ccaaagtact tcatttgtag ttccttaact tgacgctctt gggctattat
    25201 ttcatcctct cgtttctttt tcatttcttc ggtcagaaga actttttcgg tttggtaatc
    25261 cttgtacatc ttgtcaacct cggcatactt ttcctcaatt tctttctggt attgctctga
    25321 aagcttgtcg agttgttcct gtgcggcttt atacgatggt atttttttaa gtatgtactc
    25381 ggtatcgacg taggcaaatt tttgagccca agccccaagt ccaattgtaa gtgcagctaa
    25441 tatcagaatg gttctcttca taactttttg atatttggtt caggttaaag ttacacaatt
    25501 tatatgccaa gtttaacagt tttaaccgta aggattaaaa aggcatccca ataatgaagt
    25561 ggaattgacc cttatgtgca tcgggacgtc cgggcacctt atcgaaacca tatccccagt
    25621 caacgcctaa cattcctaac atgggaagga aaaagcgagc cccaacacca gctgagcgat
    25681 gaacgttgaa tgggttaaaa tcgccagcgt tctgccaggc attaccagct tcaaggaagg
    25741 taagcaggta aattgcagcc tgcggcttca gtgagattgg gtaccgcagc tcaacggtgt
    25801 acttatcgta taggtgtgca ttcccaacta ggctctcacc tatgcgaacg tatggtgtaa
    25861 gtgagccgtt ctggtaaccc cttaaaccaa tggtttcctt tccgtaaagg ttgtaccccg
    25921 acataccatc accgccaagg tcaaaaccct caaatggcga atggcctagt ttgctattaa
    25981 agtatcccat tacccccatc tggaaattgg tgtaaagaac caagttctca accagtggta
    26041 tataccattg tattcttcca gtccacttgt ggtattcaat ccatttgtac ttttccgagt
    26101 cggtcatggg tttactgtaa tccttgccat tgaaaaggga gtagggtggg gtaatttgaa
    26161 gcgagagcga aaagtttgag ccggttcggg ggtatatcat ctggtcagtt gagtttcggc
    26221 caagggtaac tttgtaacta aggttgttcg actgcccatc ggtaaagata aagtagcctg
    26281 accagttgct cagcttatag ttttgaattc ccacctcatt gtaaatggta aagtaatcgt
    26341 cgggccattt caaacgggtg cctattccaa ctgaagctcc gcttacccta aagtatttat
    26401 cgctcgactg gtagaagtag ctaacaccac ccgactggat agttctgtag agcgaaaatg
    26461 agaagttagt tggcttacgt ccgcctagcc agggttcggt gaatgaaagg ctaaacgcct
    26521 tatagtatgt tccgttggtc gaagctctga ttgctaacga ctggctatcg cccgatggta
    26581 ttggacgcca ggcatcttta tcgaaaattc tccgaaccga gaagtttgag aaacggatac
    26641 caatggttcc cacaaacatc ttgtttcccc atccacctga aacctcaagc tggtcgttgg
    26701 ccttttcctc aaccgagtat tttaggtcta cggttccatc ggcctggtta ggcaggggat
    26761 cgattcctag cttttcgggg tcaaagtggc ccatctggcc aagttcccta agggtacggg
    26821 taatttcgga tttactgaac aggtatccgg gttttgacca tatctcacga cggattacat
    26881 gttcgttagt ttttgtattc cctgtaatta ttaccctgtt taccgttgcc tggtcccctt
    26941 cataaattct catttcaagg tcaaccgagt cgttttgaat ctgaacctct acaggctcaa
    27001 gatgaaagaa aaggtatcca tcgtccatgt aaatggttga gattgagttt tcatcggtga
    27061 aaaggcgttt ttcaagtaag tttttgtcgt acacatcgcc tttattcatc cccagaacgg
    27121 cagtgagcgc ctcggcaggc aatttggtat tgccaaccca ctcaatcttg cggatatggt
    27181 attgtggccc ttcgaaaatt ttgaagtgga taccaattct cttggggttg atggtgtaaa
    27241 tggaatcgcc tagaatttta aaatcccggt agccgtgctc gttgtaaaag gcaacgaggt
    27301 tatccaaact ttcatcgtag tctgcttcaa tgaacttagc cgatttaaag atgttgaggt
    27361 cgcggcggtg tatcttttta agtgcccggc gtaggcgtgc ctgaccgtag gctttgttac
    27421 cctcaaaggt gatttcacca attttaaccc tgtcgttctt ctggatattg aaggttagct
    27481 ttgttccgtt gcttattatg gtgtcatttt ccagtacggg attaacatca acgttcagga
    27541 acccttttgc cctgtaatgt ttctttatta tgataatgct attttctagg gtggcttgag
    27601 ttagctggga gccgcttcgg agtttaagtt tatctttcag gtcgtcggcc tcacctttgc
    27661 gaatgccagt aaaatttact gtagttaggc ggggttgctc agttaggctg atctcaaggt
    27721 aaatatcatc gccttcgata cgagaggctt taatatccac atttgaaaaa agtccttggc
    27781 cccacagttt tttaactgct ttggtaattg cttcaccagg aacctgaatg gtgtcgccaa
    27841 tactaatagc ggtcatggag attaaaacat caggatttag aaactttacc cccgatacag
    27901 tgatctcctt aacgcaatat ctttttggaa gttcgtagct aaacggtttt tcctgggcaa
    27961 atgctgaaac ccctataaag aacatggcaa gtgccaaaag cctaaatctc atcatatcaa
    28021 attagttact cttttatttt gacgcgtaaa attagtataa tttcctatgt tacaaagcaa
    28081 aatgccgaaa gtattataac cttcggcatc aaaaatatga ttttatatag ccaatctcca
    28141 ataactactg ttaaaaatta cgaacgttta aagttgctca ctggtttttc caaaccgacg
    28201 ttcacgtttc tggtaatcaa ctattgcttc aaaaaaatcc tctttggtga aatcgggcca
    28261 aagaactggg gtaaagtaaa gctcagtata ggccaattgc caaagcagga aattgctgag
    28321 cctcagctca ccgcttgttc gtatcagcag ggctggatca ggaatgtttg cagtatatag
    28381 gtatttagaa ataatttcag gggttatttg gttgggcgat attgttccat ccttggtttg
    28441 agcagcaatt ttttttacgg cttcagtaat ctcatggcgc gagccatagt taagggccag
    28501 aatggtagtt atggtattgc agttagcagt tcgtgttata acatcgttaa gtttacgctg
    28561 tacatcaggc ggtagcatgg actgatcgcc tatggtcagt acgcgaatcc catttttcag
    28621 gagtgtatcc gtctcggagt tgatagtttc aaccaaaaga ctcatcaagg catctacttc
    28681 cgacttgggg cggttccagt tttcggttga gaaggcaaaa agtgtaagat acttaacccc
    28741 aatttcgcct gcggcctcag tggcagcctt aaccgctttt acaccgttct tatgtccata
    28801 aattcgctgg ttgccacgct tcttagccca tcgaccattc ccgtccataa taatggcaac
    28861 gtgctcaggg actctgctcc tgtcaatttt atcaattagc gacatgactc tatctatatg
    28921 cggggcaaat atagctgtta tttggaatac gataggtata aatcaatcca ataaatgaga
    28981 accaatcgtt attgtgtaaa aaaggtttgc ggccatcgtt ggggctgctg taatcgtcaa
    29041 ttttatcggt aaaggttttg tgaaacctcc attccagggc aaaggtgtgc ctttgaccag
    29101 gggtaaattt taaacctacg ccaaaaggta tgtgaaaaat taacgaggta cccatttgtg
    29161 caagcccaac cccagcattt acatatggcg agaggtttgc tttgcggctg ctggttgggt
    29221 ttatggagta aaagttaaat tcgcccattg cttcagctcc aattatattt tttgaaaagc
    29281 tgatgttggg atcgggaatt atatttgccg atgacgacga gccgctaaca ttggcataaa
    29341 ttgccgatgc ccgtatggag tagttatgat ttatgttata ccggaaaatt actcccatgg
    29401 caaatgaagg gctatagagt ggctttacct cgttaaactc accgtagtag taggtagttc
    29461 ctgcctgaat cccaaaatcg cgtcggtcct gtgcttggcc agctcgtatg gctaagcagc
    29521 aatgggttaa ggaaaatagt aaaatgctta ttttattcat taaaattgcc aaaatgagcg
    29581 tttaagtaat ttttggtaag gaattttgta gtaaacgtta aagtttatgg tatagtacat
    29641 atcgttgtgg tttgagtata ccgacgagaa tgcatcgagg taatcggtgg ttacgtaacg
    29701 ggctccaagc tcaaccccta tctgaaaacg gggataaaac tcatactttg ctcctacacc
    29761 aacaggtatt accagcgtaa aattctttga atcgtcaacc tcgggtttag taaatcctgg
    29821 tggtggtgtt ctggatagta gcttttcgtt gggtgttgca ctgaaaaaca aaccaccggc
    29881 acctgcaaat acgtaaaggc taaatggctt attgatatgg cgtaagcctc ccctgaggtc
    29941 cataatgcgg taatagtaat tctgattgct ctcgggaatt atgtagtaaa tccctttaag
    30001 ggttaactcg gaataaaaag tagaaaaccc atagtctctg gagctgtagt acgagccatc
    30061 gtcggtgtta ccaaggaaac ccgtattcag gcttgcttgt gccgatatga atggattaac
    30121 cttaaaacca acacctccgc caaaaccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    30181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    30241 nnnnnnncaa ttttgtttca accaccagtc agtttctttt gtccatgccc catagcaatt
    30301 tgctcctaat ggttctgtaa aaggttgttc cttttaagca gatgacattg gcattgaaat
    30361 gagctttatt aagcataagt ttaacgggtt gtgggactct aatgtttctt gaatccaggg
    30421 aaactatcag ctcttcgtct ctccctgaca cttcaagttc aattaggctg tcgtcaggaa
    30481 ttacaagggg acggacggtt aaatggtgag gggcaattgc cgaaagtaca aacgccgata
    30541 ggtttggggt aagtattggg ccaccaacac tgagcgagta tgctgttgaa cctgtaggtg
    30601 ttgcaattat taacccgtcg gcccagtatg tgctcagata atcgccgttg atgtaggtat
    30661 taacatggag tagtccagca acggagttac ggataaccat atcgttgaga gcgtatggga
    30721 atggatcaaa aagtttactg tcgcttacaa gctggagcat gcttcgcttc tcaatggagt
    30781 actttgacgc catcagcaga tcaatggaat tgtcaatttc agggattgac gttgttgcca
    30841 aaaagcccaa cctacctaag ttaattccca taatggggat tcccatatcc tgaactaggg
    30901 ctatagaatc aagaaaggta ccgtcgccac caaggctaat cattacatcg gtttccttta
    30961 gatcctgagt agtgataaac gtgccgccga tatgtggtgt gtacgatagg tttgatttta
    31021 tgtaatcgag tagtggaagg taaatcaaaa cattgacccg atatttttcg agtgctgcaa
    31081 aaagatttcg ggttgcctcg gcaaattccg catctattct tttcccgtat actgctatgg
    31141 tcatttgctt atatgtccaa aaatttgaat agataattta gcctatcgtt tagcaagtca
    31201 ctttcgtcat cctgcttcat gtatgctccc agaacgttat agttataacg ctgaaaggtt
    31261 tgctttattg aggtaacatc ggtgacatta agtttgagcg ttagctccat ttgggtggag
    31321 tttgggtgcg aggtaatata agcggcaaga atttttgcat cgtttccctc cacaatctga
    31381 caaatttgcg acaagctaaa atcgttaacg ttcatctcaa gaacaataac gcttccctta
    31441 tgcctgaggg ctgaaagttc cgtaaagtaa tgcagaagtt ccattagggt gattacaccc
    31501 aagtattttt ttgagctatc gagtactggt actaacgata gctttaggcg cgatagtacc
    31561 tccattacct caaaaacatg ttggtcctca attacatatg gtcgcaaaag cgaaagctga
    31621 tggttaccaa ggggctcctc gggcatgttg aggtcataga tatcgctctc tgatattaag
    31681 ccaaggaatt ctttatcgtt tacaataggt aggtgcgaca ccttaaaaat atccatccag
    31741 ttcaaagccg ttattccggt atcggaggtt ttgagtggag gaataacatc ggaaatcatg
    31801 tccttggcta ccatatttct tgtaaaaaac gattactttt gtacaaagtt aaacttattt
    31861 gattgcaatg acacgtttaa gtgtaaatat aaacaaaatt gccaccttac gcaatgcccg
    31921 gggtggaaat attccaaatg tgctgaaagc agccattgat tgcgaacgtt ttggtgccga
    31981 agggattact gttcatcctc gccccgatga gcgccatatc cgttatcagg atgttcgcga
    32041 tattcgcccg gtaattacca ccgagtttaa cattgagggg tatccttcgg aatcgttcat
    32101 agagttagtt cttagtgtta ccccacacca ggttacccta gtacctgatc cacccaatgc
    32161 ccttacctcc aataccgggt gggatactat tgccaacaag gactttctta aaaaagttat
    32221 tgctcgtttt caggagcatg gtatcagaac ttcaatattt gtgaatgcgg atcccctgat
    32281 ggttaagcat gctgccgata ccggaacaaa tcggattgag ctatatactg agccatacgc
    32341 tacacatttc cctgaaaatc ctgcacaggc tattgagcca tttgtaaaag ctgctgaagc
    32401 agctatggaa gttggccttg gattgaacgc aggccacgac cttaacctcg acaacctgaa
    32461 ctactttgcc cgaaatattc ccaacctgct cgaagtgtcc attgggcatg cacttatttg
    32521 tgatgccctg tactacgggc tggaaaatac cattcagatg tacctccgtc agcttcgctt
    32581 ttaaatggaa ctcttttaca ggcagcttgg acagggtaag ccattagtaa ttctacatgg
    32641 cctttacggc ttgtccgata actggatttc catagccaaa aaccttgaaa gtagttactc
    32701 tctcataatc cccgatcagc gaaaccacgg gcaaagccct cattcacctg atcataccta
    32761 ccagcattta gtaaatgatt tgcttgagct aaccaataag ttgaaactgg atagtttcta
    32821 cctgatgggg cactcaatgg gggggcgtgt ggcaatgctt ttccaatcgc agtatccatt
    32881 caaagtggaa aaactggttg tggttgatgt tgccccgtgg agctatagcc ccgaggatga
    32941 gtggttccga tcagcgtatg cagagcacat gcgaattatt gaggccctaa aggctttgag
    33001 gcctgacgaa atagcctcac gtatggatgc cgataaggta ttgcagcaaa acaatctcaa
    33061 cgagaggttg agaaactttc tgctaaaaaa ccttaaaagg aagtcccttg gatttgagtg
    33121 gcaaataaac ctcaatgcac ttgaaaataa cctggaacac cttttactgg gtgttgaggt
    33181 tgacaaaaca tcggcattgg gggccaatgt gctgttcatc cgtggcgaga attcaaacta
    33241 tatacctgtc tatcaggtag ataggctaaa agaaatttac cccatgtgcc aggtggttag
    33301 tattaccgat gctggtcact gggtgcatgc cgaaaagcca caaatgtttg tctcggcagt
    33361 tacgagtttt ttgggttaaa cctcggagcg gcttacgttt tcgggcgaaa taattggaaa
    33421 accgttatac ctcaggaaaa gctgtgctac tgttagcaca tccttttcac agtaaattac
    33481 aatccggttc aaatcgttaa gctcatagta acacttagcc acctcgctgc cgtctatatc
    33541 atccttaggt gagggaatac caaagatgta tgatagcagg ttcagcgaag tgtaatgctt
    33601 gaaatcgcca aacttccaaa gttccatggt gtcgaggaat tttacctccc atggtttttt
    33661 gccagctacg ttaagtatat cgggtatctt aattccatta accagcattc ggcgggcaat
    33721 gtatgggaaa tcgaattcct tgccgttatg tgcgcaaagg tttaggtttg gttttcgggc
    33781 tgaaaacccg tttaccattt cggcaaagct tttgagtaaa tcaacctcac tgtcaccata
    33841 atatgatttc actctgaaac cgttagtgcc attggatggc acaataatgc ccacggaaat
    33901 acaaataatc tttccaaact cggcataaat tcccgctcgc tcatatattg cttcgggtgt
    33961 ttcatcgggc tttgccaatg tttttgcctt cctatcccat agttttttga actcctcagg
    34021 catttggttg tactcagggt attgaggaac cgtttcaatg tccaggaaaa gaatttcatc
    34081 gagtttaacg tcagtgagca tggctacagc aaattatgtg aacggagtgt cttttcaatt
    34141 attgatgtta gaatatttat ggcaaccgaa ttgtttccgc cctgaggcac aataatatcg
    34201 gcataccgtt ttgtaggttc aatgaactgc aggtgcatgg gttttacggt tttttcatag
    34261 cgctcaagca ccttgttaac tgaacggcca cgctccacaa tatcgcgggc tattacacgc
    34321 gacagcctat cgtcggcatc ggcatcaaca aaaaccttga tgtccattaa cttccggagt
    34381 tcttcgcagg taaaaatcag gataccctca ataattacca cgtggcgggg ttcaatctta
    34441 atggtttcag gagctctggc gcatgtcagg taggagtaaa tgggttgttc aacgggtttc
    34501 ccggctttca gatccttaac atgttgaacc agcagctcaa attctattga atcgggatgg
    34561 tcaaagttca tttcctgacg ttgctctaag ggtaagtgtg agttgtcgcg gtagtaggaa
    34621 tcctgaggaa taacaacaac ctcaccattg ggaagcaaat caataatctt gtttacaact
    34681 gtagttttac ctgagccggt accaccggca ataccaatta ttagcatcct atcaactttt
    34741 agtgttacct ttgcaaaagt agcaatgcaa gttaattatt taaatagttt tcagcaaaaa
    34801 taaactgttg gcaatgagca attcattata ccctttaaag tttaaaccta tccttaagga
    34861 gcgcatttgg ggaggtacca ggttgcacca ttccatgggt aagcaattcc caaaccccga
    34921 agaaaaatat ggtgagagct gggaagtatc gggagttgaa ggcgatgtgt cggttgtgca
    34981 gaatggtttt ttggctggta ataccttgaa tgaactgatt gaggtttaca tgggcgacct
    35041 tatgggcgaa agcctttacg agaggtttgg cgaggagttc cccttgctca ttaagctcat
    35101 cgatgcttcc gaaactcttt caatacaggt acaccccgac gatgaaaccg ctcgggaacg
    35161 tcatcatgcc tacggcaaaa ccgaaatgtg gtatatcatt gatgccgata gggatgctct
    35221 catatacact ggatttgaaa aagatattac ccgggaagaa tacattcagg cggtaaagga
    35281 ggggaacctg gagcgttacc tacataggga gaaagccata cccggcgacg ttttctttat
    35341 cccggcaggc agggtgcatg ccattgggaa aggagtactg ctggccgaga ttcagcaaac
    35401 ctccgatatc acttacagaa ttttcgattg gaatagagtt gatggctcag gcaaatcccg
    35461 tgaactccat accgagctgg ctgtggatgt aatcgatttc gccagggttc gcaaccccaa
    35521 atctcacccg gtggatgagg tgaataaaac aagtgagcta tccgcttgta agtactttac
    35581 cgttaacagg cttaccttta ataacgcaat gcaacgcgac tataacatgc tcgactcctt
    35641 tgttatttac ctttgtgttg agggtgaatg tagtgttgtt tacgcatcgg gctccagcga
    35701 gcggctaact aaaggcgaaa cgctcctttt gcctgccgat ctgaaggata tcattttacg
    35761 accgcagggg aaatgctcct tgctcgaggt gtatataaat caggcatgag ttttgataag
    35821 gtaggtagta tgtattgtta aaaattcata tagttcgtgc actaaaaaaa tcattttatc
    35881 attaaattgc attgtttcat aaaaaaggaa gacaagttca tgagataaaa ttttgcggga
    35941 taaaaattat ttacttatct ttaactgata tttttgcgaa tggatagcgt catttatttc
    36001 ataaaatcga ccctagcaaa tgaaatcgca gcaagtggtt ttcccctgat atataagggc
    36061 gaaatgaacc accaaatcat gcgttcattt gcctttatgg caaataggaa gattgctgaa
    36121 atgaatgtcc ccactgcaac ccgcaaaagg gttttccaca tcatgattga atgcttgcag
    36181 aacatcacca aacannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    36241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncccgca
    36301 aaagggtttt ccacatcatg attgaatgct tgcagaacat caccaaacat tccgacgatt
    36361 acgatgaaaa agagaaacag attggcaatg gtttatttgt ggtggggaag gaacgcgatt
    36421 ccttttacat tgtaaccggc aaccttgttc ggaacgataa ggttcccatt ctggagcaaa
    36481 ggctcgatag gttaaatagc gccagtccat cgcaacttaa ggagatgttc atgcagcaga
    36541 tggttgaagg agaacttaat gagaagggtg gagccggttt aggattaatt gatattgcgc
    36601 gcaaatcgaa acgcaaacta tattaccatt ttgttcctta cgagaataat cgccactttt
    36661 tcatacttgc tgttactatc ccaatatcag ccgaagagga cgatttttaa ttttttcatc
    36721 attcataagt tgaaaaggta tggaatttgt ataaaatcat ttgatttatt ctaactttgc
    36781 cgtaacagtt taccaagtaa acatttttac atgaaagctt tgagtatttt tttgtttctt
    36841 gcgggtattt cgtttgggtc tctaactgct caaacaataa ccgaactcat gagcaaggat
    36901 aaaattgctc agaacaaaat agaaaccatt acccagtaca ctcaccgttt acagaacaat
    36961 aaggttgata gtaaaggcta taaatcggtt gagaccacat acgaccacaa tggaaatata
    37021 gtggaagtgg ttaactacaa acccaatgga gtagttgcct caaagcattt gtataaatac
    37081 gacgatagag gacgaaaaat tgagtaccaa cagttccaag aactccccaa aaaaggattt
    37141 gccttggcat tccgtcaagc ttttacctac gactctaaag gcaataagct taccgaaata
    37201 ggttacgatg gccaaacaac ctaccgtgtg gcttactcct atttgcccga tggcaagcaa
    37261 aaggatatta ccaaatactc ggcaaacaat gccgtggagg agaaatgggt ttttgagtat
    37321 tcaaccagca aggttaaaat atcaatttat aagcccgcag aaactttaaa caaaatcatt
    37381 gagcggactc tcgattcgca ggggcgggta attgatgaaa aaaaccttac ccctgccggt
    37441 aaggagttag gccgaacagc ctttaagtat ggcgacggcg atatgctatt aactaaggta
    37501 gagtactacg gaggtgagct acgtgctacc tacgattatg tttacgatga aaaaaagcag
    37561 atggttgagg tttacatgac taaacctggc actagcaaga tactttacag ctcatacaag
    37621 tacgataaaa acggcaacct gcttgaggaa aaatggtacg aggacggcgc ttcggactat
    37681 tcgcgccgca actacaaggt ggataaccag gggaatatca atgaggttga tgcctactac
    37741 tccgactaca agtaccgcgt tgtttacagg tactcctata agttctccag gtaaaacatg
    37801 ctaactgatt tatacgcaaa gggtctaaaa agaccctttt ttgcttgctt caatatcaat
    37861 aaattaataa cttgctggtg tttttaaatc aaaggggctg tgaggcatta tttaaaactg
    37921 tttctgtttt ttgcattagc aattacaagc attagccttt ttgcacagga aattggccgc
    37981 tatccgcttg tcaacttcca tcatcggcag tataatggac attcgcagag ctgggcaatt
    38041 acccaggaca acagggggat gatttacgtg gccaataatg ttggcgtaat tgagtacgat
    38101 ggctccgagt ggaagcatat ttcaattaac aatgcccttg cccgatgcct agacaccgac
    38161 cccactggca gggtttgggt tggaggtcag gatgaggttg gataccttgc cgccgattcg
    38221 gctaactcac ttaagtacta ttccctttct aagctgttgc ccgagcgctt tgccaagcct
    38281 ggtcttataa ggcaaatcta ccacaccggt caggcagttt acttttcaac ttttaaagct
    38341 ttatttagaa ttaacagcga tttaaccgtt aactactggg agcccaaaac ccagttccat
    38401 cgctcatact atgtggataa tttcattttt attaaccagc gcgattacgg cttaacctac
    38461 ctttataacg attcactggt aaaagtaccc ggaaccgaaa ttcttggtac acaaatggtt
    38521 tacaccatcc tgccattcga ttaccagtac ttactggttg gaacgcaaac aaacggattt
    38581 taccttctta ataagcaggc actaaccact cgcaccttac ccaccgattt taaaacgctc
    38641 atcccgttta aaacatcaaa cgataatttt tttatcacca acaggattta ctctggaata
    38701 atgcttgatg agggactttg ggctattgga acctattccg gaggcgttgc cattatcaat
    38761 cacagcggga aaattgttag aaccatcagc aaggaaaccg gactacagga caataccgct
    38821 tggtatttat atgtcgataa ctccaaaaat atttggatgg cattaaataa tggtatatcg
    38881 tactcgcctt ttcattcgca gctaacaagt tggactgagc aggacggaat aagcggggtt
    38941 atacagtcgg ctgtaaattt caagggaacc atttactact cttccaatac gggtgtccac
    39001 gttaaaacaa attcgcactt tgaaagggta agcggcatta acgatttagc atgggggctt
    39061 ttcatctaca ccgatgaaag cggcaaggaa cacttgctct caggaaccgc aaacggaatt
    39121 tataggattg aaggagaaag agccatccca gtaacaagta ttaaagtaaa tgcatactcc
    39181 tttattaaat ccaggattta cccaggcatt ctctatgttg gcctttacga tggggttggg
    39241 gttttgcagg agaacagggg agtattcacg tttctaggat atttgtatag ttcatcatct
    39301 gaggtttaca aggttaccga ggataaaaac ggagatgttt ggtttactca gcgctataag
    39361 ggcataggtt tcctggatgt cataaaccct tatgagctga tagccgaaaa gtataaaagt
    39421 tactctttac catttcaacc caaatgcgat gatattgcgg ttaactatat cgatgggctt
    39481 gtgctggcaa gcaccgaggg tgggctttcc agctacgata aggataccga ctcctttgta
    39541 cccgaaacca tgctgggcac tcagtatgcc aacggtacta ttgggttaag aatactggca
    39601 tgcgatagga ataacgacat ttggtttgaa acctatagat ttcaggcaaa ccgtaaactg
    39661 gaaagggtta ccaagcatgc cgataaagcc tacaggcgtt cccctgccca gtttaacctg
    39721 ataccggaga ccatcttttt tgatgttttt accgattccg ataatgtatc gtggatttca
    39781 tcgaccgacg gactgttccg tttcgatgcc aacgagcact ttcccaccga tcaaatacct
    39841 agggtattta tcagacaggt atattcaaag aaaaagggca cggtttacgg agggttttct
    39901 tttgacagcc ttgcaactca accttttgtg cgggcaaaat ctgttcaggt tcagcaacct
    39961 attgtactgc ctgccagggt aaacgaccta attatctttt tctcctcgcc ctacttccag
    40021 ccaaaccacc agcttcagta ttcgtacaag cttgaaggtt acgatgacga ctggagccca
    40081 tggaatacaa gcagattcaa ggagtacact aaccttaagc ccggcagtta ccggttcatg
    40141 gtaaaatcca aaactctaca ggatatcgaa agccctgttg agtattttcc gttcataatt
    40201 gaccatccct tttacctgaa gtggtacgct tttattctat accttgcttt ggcggggttg
    40261 atcattttgg taattgttag gattaacacc cgaatattga gggtttcaaa catcaagctg
    40321 cagcagctcg tcgatgagcg aaccaaggag ctgaaggaat cggagcgagc actcaccgaa
    40381 aaaaacttac agctccaaca ccaaaaggaa gaaatccttt cgcagcgcga tgagctggag
    40441 gagcgtaacc gtcatattaa cgacagcatt cagtatgcaa agaccatcca gcaggccatt
    40501 ttgcccgatt tggatacggt gtttgcaaat gattttgagc actttctcat ttaccttccc
    40561 aaagagatgg tatccggtga tttttactgg gttgcacagg ccggacccaa gggaaaacca
    40621 aacgataagc tctttgtagc tgttgtggac tgtacggggc atggtgttcc tggggcattc
    40681 atgtcgttaa taggcagtcg gttgttaagt gaaattgttg tggaacggaa aaaccacaac
    40741 ccggcagcca ttcttactga actaaacgat gaggtaaaca aggccctacg gcaggatgtt
    40801 agcgagagtt ttgatggtat ggatgtggct ttgtgcctaa ttgaacgcaa atccgataat
    40861 gcctatgtgg taacctttgc cggggcaaac cgccctctat actactgcct gaagggcgat
    40921 aataacatgc aaaccctaaa gggtaaccgc aaatccattg gcagcgtgct gcccgacgtg
    40981 gagcaggaat tctcaaactg gcgtatctcc atgcatgcag gcgatatact ggtaatggcc
    41041 accgatggca taactgacca gaataacgtt ttgcgtaaaa agtataccac ctgccgctta
    41101 cacacatcca ttgtttcggc actgggtaaa ccaatgcccc aaatgcaaag catcattctg
    41161 gacgattttc tgaacttcaa ggccgacgaa ccccaaaggg acgatataac cgtgctgggg
    41221 ataaggttta agtagggtaa gttttataag tcaacggctg ggggttggta aagtgcagga
    41281 gtttgaaacg atttcctatc ccacgct
//
LOCUS       DAOSAL010000016        40829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_731_length_40829_cov_48.173566, whole genome shotgun sequence.
ACCESSION   DAOSAL010000016 DAOSAL010000000
VERSION     DAOSAL010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 40829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40829
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_731_length_40829_cov_48.173566"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            558..1517
                     /locus_tag="PK049_03935"
     CDS             558..1517
                     /locus_tag="PK049_03935"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOK60741.1"
                     /translation="MDNRKLILIGLVVTAVCFFVLMLSFIPRPNSGFERYKSLNPWSD
                     SVYQSSLIFAIPIPENLSFAGEEVPLQYFDVRESLDRELHVNTFWHSQTVLLLKRANR
                     YFPIIEPILKKNNIPDDFKYLAVAESGLTQAVSPSKAVGFWQILEGTAKEYGLEINKE
                     VDERYHIEKSTEVACQYFKKAYEKYGTWTMAAASYNFGMNGITKQIDRQDSNSYYDMV
                     LGDETGRYVYRILALKVIFENPKQYGFFLDSTDLYPPLEYTEVKVDSTITSIASFARQ
                     YGVNYKLIKLFNPWLRENYLPGKQGKSYLIKIPRKGFREEAYK"
     gene            1526..4384
                     /gene="uvrA"
                     /locus_tag="PK049_03940"
     CDS             1526..4384
                     /gene="uvrA"
                     /locus_tag="PK049_03940"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOK60742.1"
                     /translation="MFQDIDKSALTDEGEIVVEGARVHNLRNVDVTIPRQKLTVITGL
                     SGSGKSSLAFDTIYAEGQRRYMETMSAYARQFLGALERPDVDKISGLSPVISIEQKTT
                     SQNPRSTVGTITEIYDFLRLLYARASTAYSYQTGEEMVRYTDEQIINLINERFTGKRT
                     AILAPVVRGRKGHYKELFDQLRRKGYLNARIDGEITELRPAMKLDRYKTHYIELLVDK
                     FRVGEGDLKRLSQSVATAMNIGKGIIMVLDMDNGEYRYFSRNLMCPTTGISYDEPEPY
                     TFSFNSPKGACTHCNGLGYINQADIERIIPDPQLSIKRGGIQPLGPYKNSLIFWQLEA
                     IASKYDFSLSDPIGQIPDEALNVILYGSEEQFKLKHPSIGVNSSYFLSFEGVISYIQN
                     HQTNDELNGKGDRWANQFIKQVTCPTCKGTRLKEESLWFKVDGKSIADVSAMDIETLW
                     HWLQGLENRLGSRQKAIATEILKEIRERVRFLLDVGLGYLSLNRGSSTLSGGESQRIR
                     LATQIGSKLVNVLYILDEPSIGLHQRDNIKLINSLKQLRDAGNSVIVVEHDEEMIRSA
                     DYVVDIGPRAGRHGGKIVATGTPSEILMSNSLTAQYLNGQLKIPIPKERRKGNGKFLT
                     VKGARGNNLKNITVKFPLGTFICVTGVSGSGKSTLINETLYPALSRHLYRSYAQPLEH
                     DAIEGIENIDKVIDVDQSPIGRTPRSNPATYTNVFTDIRKLFELTPDAKIRGYKAGRF
                     SFNVKGGRCETCKGGGVQVIEMNFLPDVYVKCPDCFGRRYNRETLEVKYKGKNISEVL
                     DMTINQAVEFFDSIPQIHQKLKTLQDVGLGYITLGQQSTTLSGGEAQRVKLATELAKR
                     DTGKTLYILDEPTTGLHFDDVRILLEVLNKLVDRGNTVIVIEHNLDVIKSADYIVDLG
                     PEGGAAGGQVVCTGTPEDIAKSGCGYTSEFLTKYLFRE"
     gene            4442..4819
                     /locus_tag="PK049_03945"
     CDS             4442..4819
                     /locus_tag="PK049_03945"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOK60743.1"
                     /translation="MDTLKIEKRIDSPMVLADGESGYFEVNGKSLPEDAIEFYKPLEK
                     YAQEYVKSPKQKTTINLKLEYLNTSSSKKLLDIIGYFEALPSQGYEVVLNWYHRDEDQ
                     DMIDEGIEFAHMTSLKVNFIVEQ"
     gene            4828..6729
                     /locus_tag="PK049_03950"
     CDS             4828..6729
                     /locus_tag="PK049_03950"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOK60744.1"
                     /translation="MFRFVQYYVLLILFAFAHFTAQSQTTPLGSSKELQNSLQQAKEL
                     ESNGDLNGALSIYNKTATAYWVNGKLTEARDIFLKAASVAEKLGNQNALKVIYTNLGM
                     VFVDLEQYSEAIAQFEKSLAINRSQKNKAETTATLINIANAYKELDKYQKALDYAEQA
                     NTIALEINDAKLLRNTYSLMAEIHEALGNSEKSSEYFSLYTAFSRKIQREEQLKKEAE
                     AQKIVEEAKGELQVIKSEKELTEKELSEKQRVLETVKDSLDKIEQLTQEQKLQIDLLN
                     KENALREEKIKNQILVRNIFIVIIAATIGMLGLITHYYLQKKRSNEELARQKAMVEAK
                     SQELMQALQQIEKQNRDITSSINYAQRIQQALLPTYDQLVSYIPNAFIMFRPREIVSG
                     DFYWFAAYGGQNIAASKQNRHMIALPNIPKNDFGFLIAAVDCTGHGVPGAFMSMIGLN
                     LLDVIVRSGLTQPNLILNELHKQVRYLLKQENNDSRDGMDMALIRIKGDGRTIEFAGA
                     KNPIVYITNNQCFTIKGDPVPIGGLQKEEKREFTLHTITIDSPTNFYLFSDGYIDQFG
                     GPENLKFTSKRFREMLFENHNLPMVAQQEKLETALDEWIGSENNQIDDVLVIGLHLTG
                     KPFKIDSQT"
     gene            6779..7708
                     /locus_tag="PK049_03955"
     CDS             6779..7708
                     /locus_tag="PK049_03955"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOK60745.1"
                     /translation="MSKQYFGVLAFLFLFTSALAQENANNVYVNNNGDVFVKADAPIF
                     FFISPENQPSQKVLIPSTDKAANPMYFDGDGKHYLVYEGKGEKVRFLIWADGVGPKSS
                     LRVEKGLLFNHNNRVYVEDRASFTPSAIDSKSGVKQSFIAVDNQPFAPFNGNVDIERT
                     GEYTIKVYSTDNVGNVGDTVVYKIVAAPDVTFKIDNIYFETASARITGNSINNLKEMV
                     EILKNYPELHVEIKAHADSRGSSEYNLELSQRRAQSVVNYLTSRGIEGYRLKDKGYGD
                     TQPVNECVKGIKCPDSKLRENRRVEFRFYLPKK"
     gene            7717..9405
                     /locus_tag="PK049_03960"
     CDS             7717..9405
                     /locus_tag="PK049_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60746.1"
                     /translation="MNRFAQILLVTLTGFTFGSFAQDAEKKTFYIDSTGNLYVPLSQP
                     VQIYMGTEPDGSNAVLLKSINKISAPLTWNGSGPQLMSHLDLYKGRKILFELYADGIP
                     PQTSINPKSQNILEQKDAIIIRGGSIIELNATDEAAGVDKILYSINGSSFAPYNTPIN
                     LTDDGEYEIKVYSTDKLGNSEPVVSRKIIVDATPPLTEISFKGDKYEDIFSGRAALTL
                     TASDRFGVAGTKVMIDSSGKWTDYKQPIQTANLAEGYHTLTWYSTDRAGNIEEQKNYR
                     FFVDKTPPIVVEEIEGNSYMIGTREFSSGRSRLKVMAVDNKAGVKEIYYSLNNREFKL
                     YEKPVMLADILGTVKLKTYAIDRVNNRSNSEANAQSFTMPTIDITGPELGYRLNGPKL
                     VLRDTLWIGPETKIELLATDREAGVNRIEYALNQGQNKQYLEPFVLTDDGFYKLEFTG
                     YDNVENVNVSGFTFAVDTQAPEIYWHFSVKPIGFEQVESQTLPVYPDNVRIYIGESDN
                     LTATNTIQYSINGSKNQNYNTPITNLKVGINELAIFATDALNNTRKETVRFIIR"
     gene            complement(9457..9876)
                     /locus_tag="PK049_03965"
     CDS             complement(9457..9876)
                     /locus_tag="PK049_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60747.1"
                     /translation="MKRIAKILTLTAVAFILASYAANAQNVVRVKPGHKTVVVKHKGP
                     RAKKVVVYHPRWAPKKVYYRRWVYFPKYNFYWDNVHEVYVYRNGNVWVTSVKVPAIVV
                     NIDLSNEKQVELPEENDSVDEIYKNNEQHLKIDENER"
     gene            10070..10549
                     /locus_tag="PK049_03970"
     CDS             10070..10549
                     /locus_tag="PK049_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60748.1"
                     /translation="MNLRNYTIRTLLKVTAFVVIFTLLVFTLIFLSQQRNSQRQREAL
                     ISLEHLYQQNISLKQIKDNFIYRDTRSEQLYKLGYSNSTLKHDSLIRVCKETLKQIGN
                     HYNYNQLDQIGQQLSTYNKKFYGVKELVQKRGFKDFGIEGELRAKIHAVESTVEQFI"
     gene            complement(10608..10901)
                     /locus_tag="PK049_03975"
     CDS             complement(10608..10901)
                     /locus_tag="PK049_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60749.1"
                     /translation="MKAIFIAYNQALSDKVDSALQIAGVKGFSKWADMLGAGSQKGEP
                     HLGTHTWPSMNSGMLTIVDDDKVDKLLSYINQINKVAEEQGIHAFVWNIEKMV"
     gene            complement(10920..14063)
                     /locus_tag="PK049_03980"
     CDS             complement(10920..14063)
                     /locus_tag="PK049_03980"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOK60750.1"
                     /translation="MNLYSSAVKNPISTLMIFVGVIVMGIYSIFYIPIDLLPEMEIPS
                     ISVITFYPGASAEDIEVNVTKPIENQLNALQNLKKVTSSSKDNISFVTCEFEYGINLD
                     EASNDVRDAVSIAEKSLPDGCEKPSIFKFSSSMVPVVIYGIQADRSYAALEKLIDEKI
                     IEKLSRIDGVGSVTIIGKPIRIIDVTVDPRKLDAYNITLEQVGAVIGAENISLPAGRI
                     EVGRMEFPVRTNGEFRNSDVVKSLVVANNGGKLVYMKDIATVKDTLKSVLVDERIDGS
                     LGVRLMVQKQSGANTVKIARLVRESMDELQKDLPSDVKVVKIIDSSEFIQNSINNLSE
                     TLIYAFVFITLVVILFLGRWRSTFIIMLTIPISLVGAFIYLFISGNSINIVSLSALSI
                     AIGMVVDDAIVVLENITTHIERGSSPMEAAVFATREVGLAVIATTLVIVAVFMPLTLI
                     GGLIGVFFRQLGWIVTITVSLSTLAALSLTPMLSSRMLKGRSYYKHHRSRFLESVNRF
                     WGFFDTIYVKSLAWTLKHKALTLIIAGLVFIGSLGLAGVVGTEFLPSQDNGRINVNME
                     IPQGIRIDEAKALARDFEQKVKKKYPEVTLISTSIGADEGGRLMSIFQKSASYIINYS
                     IILTSADDRTRSIFEIADSLRKDITGYTQVTSSYVDPGGNRNSRITLGMGGGSNLEVK
                     ILGYDINQTNYIAEQIAGTLEAEIGFRDISISRDKDRMELQVIPDREKLAQLGLNTSM
                     LASAVRNRIYGLTATKYREEGTEYDVILKYNDANLTSVSDIENITFKGPMGNMVRVGD
                     VAQIKQVYTTPNLEHEDKIRVVKVVSALSGIDLGTASSIINAKLATLTIPDNVIVEIG
                     GAAKDMADTFQDLKLLFVLIMVLTYLVMAAQFESLREPFIIMFSVPFALTGVFISLYV
                     TGTTLNMISGIAIVMLVGIAVKNSIVLVDFINLLVSRGRTISQAILEGGKSRLRPILM
                     TSFTTLLGMLPMALSKSEGSEIWRPLGISIVGGLVFSMLISLVIVPIIYALFANARIK
                     RERKKLQASDSEISFDD"
     gene            complement(14082..15128)
                     /locus_tag="PK049_03985"
     CDS             complement(14082..15128)
                     /locus_tag="PK049_03985"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOK60751.1"
                     /translation="MRTINTFKAITLASAVVLLASCQGNRADEKQLATTATEVKVVTQ
                     VVQPVSINRQFRYPATIQAYKENHLAPATAGRVEKVLVDVGDRVKAGDVVAMLDRTTY
                     QQAKIQFEKLKIDLQRVDSLLKVGAIPQQQYDAVKMQYDIAKTNLEFLDDNTSLKSPI
                     NGIITGKYINDGELFTMSPAPGVGKPAVVSIMQLDKLKLLVGVSSTYLNAVKPGMKVT
                     VKTELYPDKEFTGTVEKVYPTIDNMTKTFTIEVVVPNPDKMLRPGMFATAYINVGVGD
                     ALLVPAFVVLKQSGTNERYVFVYDNGKAIRRNIEQGDVFDDKVEIIKGLSAGEEIIVE
                     GQTIVFDGQPVVKK"
     gene            complement(15164..16492)
                     /locus_tag="PK049_03990"
     CDS             complement(15164..16492)
                     /locus_tag="PK049_03990"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOK60752.1"
                     /translation="MRKLITIFSLAGFVLSTSLALGQQKPDTLHLTVKQAVDLAIQQN
                     LMHKVTELELQKKQEKVKEYMAALYPNIKASASYTRNLKLPVIFMPEGSPFGPTLKVG
                     SDNSYTGGVSGSMPVFIYNVFESIKLGRKDAEISAEKVRENKINLAASIKTTYYNVLL
                     LCKTNDVIKQSYQNALANLSNIQKLNANGMVSNYDLIRIKVQVDNIYPNLVQTANAYS
                     NLLNVLKVLLNIDVNTPVKLDENELLSYNFEELVVADSSWFGRNSTLKQLALTRQMLT
                     IQERIVKGSNYPSLVAIGNYQYQTQANDFNFSNYNWVTTSMVGLQLNIPIFSGFSVRK
                     QLNQVRISQQQLDLQTQFTRNNLEVQLQNAANAVNTAVKKVNAAKGNVDLAEKGYTIA
                     QTRYNTGQATLLELNDAENAMLQAKLNLIQAQLEYLVAKTDYEKIIGESF"
     gene            complement(16514..17140)
                     /locus_tag="PK049_03995"
     CDS             complement(16514..17140)
                     /locus_tag="PK049_03995"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOK60753.1"
                     /translation="MAVKEQIVEGALELFKRMGIRGITMDMLAEHLGMSKRTIYENFG
                     NKDEIVEACVLLENESMQAMVQSILNKSEHYIETYIMFMWSHINKLRNVSPLFMYDLK
                     KNYPKTICKQAGEFDHYLKERMVEFIEKGKADNLFRAEVNPQIVASVVFEFTKLLRSG
                     FEPGSNSLNDFPLAEVFEHMAVNFIRGLATSKGIELVDYYYEKHKQIR"
     gene            17315..19039
                     /gene="lysS"
                     /locus_tag="PK049_04000"
     CDS             17315..19039
                     /gene="lysS"
                     /locus_tag="PK049_04000"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOK60754.1"
                     /translation="MALYEELSEQEIFRRQALDELRSLGIEPYPAAMYPVTHSTADIL
                     SDFENMSQNRAEVYIAGRIMARRIMGSASFFELQDEKGKIQVYVKRDDVCPGEDKTLY
                     NTVFKKLLDIGDIVGIKGFVFRTQMGEESVHAKELTVLAKSLRPLPIVKEKDGKVFDA
                     VTDPEFRYRQRYVDLIVNPGVRDIFRKRATIINTMREFFNSKGYLEVETPILQPIPGG
                     AAARPFVTHHNALDIPLYLRIANELYLKRLIVGGFNGVYEFAKDFRNEGMDRTHNPEF
                     TVMEIYVAYKDYFWMMDFTEEMLERVALAIHGTTKVTFGDKEIDFKRPFRRMTMTDAI
                     KQYAGVDITGKSEDELRAICKQMDIEIDDTMGKGKLIDAIFGEKCEEHLVQPTFIMDY
                     PIEMSPLCKRHRSNPELTERFELFVAGKELCNAYSELNDPIDQLERFQEQLRLSERGD
                     DEAMYIDMDFVRALEYGMPTCSGMGIGIDRLTMFMTNQPSIQDVLFFPQMRPEKKPQK
                     DTAEAYAQKGIPADWVPVLQKMGFNTVASLNGVAAGKLFNDLCGYNKKNKLGLPNPTM
                     DDVKRWVE"
     gene            19163..20062
                     /locus_tag="PK049_04005"
     CDS             19163..20062
                     /locus_tag="PK049_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOK60755.1"
                     /translation="MQIEIIVKKPANYNLGASLEVLIDFLSQIQKWDKGSKLIINLQN
                     IDFTYPLTLLPIASYLKYLSSLYYDIDFVYNKNTQKYLNVIKFPYGLSYDYNELTIEL
                     KKYKNKTYLPICSIPSGSNNSKIRDQILSLFETILVSQTNIKGQLKIAVSYIINETID
                     NIVEHANVSNGWIMAQNYPKKGFLDVCIADTGDGILESYKKTKIFNISTDAQAINKAI
                     NGLSTKEQGNTRGYGLKTSRKMLVEGLGGMFLLFSGNSFYIWNTNIETIFSDIPYKWK
                     GTIVAVRIPHDIPSNFNYTNYLE"
     gene            20157..20504
                     /locus_tag="PK049_04010"
     CDS             20157..20504
                     /locus_tag="PK049_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60756.1"
                     /translation="MKTKVIHIREYLPSIISTRKAIKEIRESISFDNNCKYIFDFSGI
                     FFISRSFADEFYKFVTYSHIDYSILNANKNIEEMLNAVSRISKKDIKTDTDEITFTYF
                     KNINELHSFLSSF"
     gene            complement(20543..21784)
                     /locus_tag="PK049_04015"
     CDS             complement(20543..21784)
                     /locus_tag="PK049_04015"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOK60757.1"
                     /translation="MERKDKQLAELARSGSPMRIAIRRLLADRMAVASLLLIASIAII
                     AFLGYLIIPDSSPYANGQHLQLATLKPGSKVKFLRVAKQHEVKKRNILGRMFYGQEQD
                     YNEIPVDSIYFDGIRVYASEYNPIPEGDAIVAELTLPEILFRLNPEKKYLLENDTITA
                     FLYNGQHAIMAVADAQQRVLSQNLVTRRFLLGTDRFGRDLLSRLVIGARISLSVGFIA
                     VGIALIIGIVLGAIAGFARGIIDDAIMWMVNVVWSVPTLLMVIALTMVIGKGFWQIFV
                     AVGLTMWVDMARVVRGQVLSVREKEYVEAAKVLGYGPWRIIFRHVLPNIMAPVIIVSA
                     SNFSTAILLEAGLSFLGIGVQPPAPSWGTMIRDHYGYIIMDKAYLALIPGFAMMLLVL
                     AFNLLGNSLRDALDPREIEKA"
     gene            complement(21760..22872)
                     /locus_tag="PK049_04020"
     CDS             complement(21760..22872)
                     /locus_tag="PK049_04020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOK60758.1"
                     /translation="MIIISTILIAIYTIAILWLLKGLLTNNWLESNGAPSQTVNGISV
                     IVAFRNERENLPKLIASLEAQDLPHEFWEAILVDDGSTDSGFDLVMSCKSQLPLKPLV
                     LPDGQAGKKQALLYGLSAASFDTVVFTDADCTHTPQWLSSIRESMGSTDFLQGEVKPQ
                     FEKGSIISMFEALDYLSLMAVSAASFAVGKPVIASSANLAFRRSKVNVDTKVLRTDIA
                     SGDDMFLLHHAKTITDLRFRFLASPRVAAYTSFSGGIKGFVLRRSRWASKATAYTDSL
                     TLLLSFLVFTLNFWIILLAILTVLGVITFNLPISLLAAKAIVDLPFLAYYLFKTKQQN
                     LLAVFIPLQILYPFYITFAAILGLIKGTTWKGRINS"
     gene            complement(22869..23990)
                     /locus_tag="PK049_04025"
     CDS             complement(22869..23990)
                     /locus_tag="PK049_04025"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOK60759.1"
                     /translation="MRAKPDGSYISYFSNLVKVNGGINLAQGIPGFQPPTELINILKD
                     IANSQHHQYAPGIGNLQLRALIENSYGVEPDELLVVQGATEGLSLVYTYLFRMLGKNL
                     VTLSFDPAYESYSRLPQIFNQKFVPFSLINEEFVDFDELKRCIEENKVKLIFISSPGN
                     PYGKVWTKAETCKLLELANELNFFVVFDSVYEELYFSQKPYIPLDNISEKLFIVSSFS
                     KSLCITGWRVGYVIHHPSHTKAIRAIHDYIGLCAPSVMQQALAVYLAQFQYGKAFMDE
                     LRQRVKSSFDSLSGMLNRLNFTVPKIDGGCFIWAKLPQPFTDGFEFASDLYDATGVAV
                     IPGEHFSSTKVNWLRFNVARPLEEIDLAGNRIADFMSKQ"
     gene            24111..25265
                     /locus_tag="PK049_04030"
     CDS             24111..25265
                     /locus_tag="PK049_04030"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOK60760.1"
                     /translation="MRFRYFVSIVLIVFLAGCSSKDAVVISVKVKSPEKTTLFLSKID
                     FNKTIKLDSVRISAGESVKKFKVKQGEEPEFYSLSTSKGLAITLLASKGEKINVILDL
                     NNAIDYQVSGSEDSEKVRWVTRTFAKSKTAVDELSKRYLNAESNSERELINNQFEQER
                     KRVKDELTKFVWENPMNKASIMALYQKYNDNLYIFDSSDDLLLIKMVATAWKALYPES
                     SYTKGMLEDIKNIENRIANAKMQQLIQNAEMPIPDLSIPDRNGRSIKLSSLKGKVILL
                     DFFTSENTASLLDNRELLEIYKKYKSRGFEVYQISFDATREGWINYIENAGLPWISVR
                     EDDPSASNAALFYNVRQLPANYLIGKDFNIIGKNLYGTNLQKALDSYLNK"
     gene            25274..26059
                     /locus_tag="PK049_04035"
     CDS             25274..26059
                     /locus_tag="PK049_04035"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HOK60761.1"
                     /translation="MSQGKKIYFASDLHLGLPNLEQSLPREKLFNTWLDTISHDAEEV
                     YLLGDIFDFWFEYRRVVPKGYTRFLGKIAQLTDSGIPVHFFTGNHDVWMFDYFEKELG
                     VQIHREPIVRELYGAKFLLAHGDGLGPGDPGYKLLKRIFTSPTLQWMFAKLHPNFSLW
                     FGNQWSVSSRYSKEITHVFRGEDEQITKFSRHKLQDEHFDFFIYGHWHSPIIYPLSEK
                     SNLVVLGDWLVSNTYSVWDGKELKLIRYQPPKADEVLATTTFL"
     gene            26071..26697
                     /locus_tag="PK049_04040"
     CDS             26071..26697
                     /locus_tag="PK049_04040"
                     /inference="COORDINATES: protein motif:HMM:NF016239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HOK60762.1"
                     /translation="MNFLAHLYLSGSNIDIRLGNFIGDHVKGKQILAYPQGVQTGIKL
                     HRTIDQVTDRHEAWKSCREKLRPSYGRWSGVATDVMFDYILAKEWSTYSNRPLKGFTR
                     TFYFQMLQRYHMLPPAVRGFLPFMIQSNRLYSYSTDEGVTRALEIMGNYTTMKGNPSL
                     ALQIVKENYIAFSAGLQELLYALIQVAEKEYGIPISMPHSVKHRTEGE"
     gene            complement(26654..28075)
                     /gene="rodA"
                     /locus_tag="PK049_04045"
     CDS             complement(26654..28075)
                     /gene="rodA"
                     /locus_tag="PK049_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOK60763.1"
                     /translation="MQRRNNILRNLDWVATILYVVLVLMGWLNIYAAVYSDEHRNILD
                     FSQRYGKQLVWIFFAFLMAIVILYSNDRIYPAFAYIFYGIILITLISTLLFGRVVNAS
                     KSWIPIGSFGLQPAEFAKIATALALAKLMSSYGFKLSGFWSYVKVAVIIGLPVGIILL
                     QNDTGSALVFGALILVLYREGLPAWILSYLVFLVIVFILTLVYSTPVLITFIFVVAVT
                     LFVVFTRQHRDAFRISIGAAGIGLLLYISSELFHLRLSNALIFLIPVLISAVLGFLYA
                     YKNRLRQVYAISFFLIATIGFSMSADYVFERVLDVHHQKRINDLLGIEDDPLGWGYNV
                     NQSKIAIGSGGFWGKGYLSGTQTKFNFVPEQSTDFIFCTVGEEWGFLGSTVVVLLIAL
                     FLLRLLKIAERQTEHFARIYGYGVVSILFFHAAVNIGMTIGLVPVIGIPLPFFSYGGS
                     SLWAFTILVFILLRFDASRYGAY"
     gene            complement(28059..29906)
                     /gene="mrdA"
                     /locus_tag="PK049_04050"
     CDS             complement(28059..29906)
                     /gene="mrdA"
                     /locus_tag="PK049_04050"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOK60764.1"
                     /translation="MLRAESSRRVVVSVIIMLVFAAIIGKLFYIQVIDSSYKFSAANN
                     VLRYITQYPARGLVLDRHGVPMVSNKPVFDILMVKRQVKPFDTLEFATMLGLTVEQVR
                     DAFIQATKQKGYSPRKAVVLLKQISAENFARFQEVMYKYQGFEIQPRTVRSYNPPIAA
                     HLLGYVGEVDDRDIKNNPYYQQGDYIGISGIERSYEHILRGKKGVKIFMVDVHNRIKG
                     SYEQGKFDTLAIPGKNITSTIDARLQEYGERLMQNKIGSIVAIEPKTGEVLAMISSPT
                     YDPNLLVGRQRTANFKVLQQDSLKPIFNRSIMALYPPGSTFKVVNALIGLQEGVVTPE
                     TRYSCYGGYTVGRGVGCHNHPSPTNLIQSIQVSCNTYYCHVFRNIIDKREFGSVENGF
                     SVWKRHVESFGYGNKLNIDLPHELKGIVPSVSFYDKYFRKGGWNSLTIISLAIGQGEL
                     SVTPLQMANLVATIANKGYYIAPHVVKSIEGDTLNSLYREKHITSIEPKYFDYIIEGM
                     DLAVNGAPGSGSTARIAALPDIRICGKTGTAQNPHGKDHSVFFAFAPKDDPKIAISVY
                     IENAGFGATWAAPIASLMIEKYLKDSISRPDLERYILDANLLDRRAKAK"
     gene            complement(29909..30427)
                     /locus_tag="PK049_04055"
     CDS             complement(29909..30427)
                     /locus_tag="PK049_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60765.1"
                     /translation="MYSDILKYLGLTVILLLLQFLVLNNINFMGYVNPYIYILIILLL
                     PYRITRWMLLIIGFALGFAVDYFSNTLGLHTTASLVLAYFRPVILNRWSFKSVQDIRG
                     VPDVTNTSLEWFVVYALIAVLVHHFTLFVLEVFTFKHFWLTLVRIILSTAVSTFFIVI
                     IELLRAPNKMSR"
     gene            complement(30427..31248)
                     /gene="mreC"
                     /locus_tag="PK049_04060"
     CDS             complement(30427..31248)
                     /gene="mreC"
                     /locus_tag="PK049_04060"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOK60766.1"
                     /translation="MNNLYEILKRLYIHLIFLILLGISLSLYFNTTYYQKSQVGAVTK
                     SISGLWHKQASQVTGLFQLQRSNVLLLLENVRLRNEIEGYRLKLQMLQDSFMRIEDTS
                     GLFRYIPARVIDNSINSRHNYITLDAGSKDGAYKEMGVICKNGVVGIVSATTKHFASV
                     ISLLNTDVRVSAMHKKSGAFGAVSWDGMNYRRVVLDEIPLHVNVAVGDTIVTSGFSTI
                     FPRDIPIGRVVDFYNKDGSFLQITVELFADFKTLDYVYLVDSKVKSEVDRLQTMK"
     gene            complement(31268..32287)
                     /locus_tag="PK049_04065"
     CDS             complement(31268..32287)
                     /locus_tag="PK049_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOK60767.1"
                     /translation="MGLFSFLTQELAIDLGTANTIILFNDKIVVDQPSIVAIDHSTGK
                     MIAIGEQARAMHGKTHENIRTIRPLRDGVIADFNAAEQMIRGMISMINTKNPIFTPSL
                     RMVIGIPSGSTEVEIRAVRDSAEHAGGRDVYLIYEPMATALGIGLDVMAPEGSMVVDI
                     GGGTTEIAVIALGGIVCNKSVRIAGDGFTADIQAYMRQQHNVKIGERTAEDIKINVGS
                     ALPDLDNPPADFIVRGPNLMTALPIEVPVSYQEIAYCLDKSLSKIESAIFNVLEQTPP
                     ELYADIVQKGIYLAGGGALLRGLDKRLTEKVNIPFHVAEDPLHAVARGTSIALKNLSF
                     PFLMR"
     gene            complement(32309..33835)
                     /gene="purH"
                     /locus_tag="PK049_04070"
     CDS             complement(32309..33835)
                     /gene="purH"
                     /locus_tag="PK049_04070"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOK60768.1"
                     /translation="MDGLKTIKTALISVYHKDNLDKIVKYLGEKNVTIYSTGGTMDFI
                     KGLGIPVVAVEELTGYPSILGGRVKTLHPKVFGGILARRENENDLFQLQEYSIPEIDL
                     VIVDLYPFEKTVESGASEPEVIEKIDIGGISLIRAAAKNFRDVLIVSNREQYERVYEM
                     LTEQDCATSLEQRRIFAAEAFATSSHYDTAIYDYFSPEFPQSFFRKSLKGVRGLRYGE
                     NPHQKGVFYGNFDEMFDQYCGKEISYNNLLDIDAAVNLIREFEEPTIAILKHNNACGV
                     ASRPSILQAWADALAGDPVSAFGGIIISNRSIDETTAKEINKIFFEVLIAPNFDNAAL
                     EVLQAKKNRIILRMKDFNLPQQVYRTALNGVLMQERDSVTESDSMLQLVTQKSATPNE
                     VNDLLFANKIVKHTKSNAIVLVKNLQLVGSGVGQTSRVDAVKQAIAKAQSFGFDLNGA
                     VMASDAFFPFPDSVQLAYEAGVRAVIQPGGSIKDQESINFCNANGIAMIFTGIRHFKH
                     "
     assembly_gap    34244..34343
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <34344..35567
                     /locus_tag="PK049_04075"
     CDS             <34344..35567
                     /locus_tag="PK049_04075"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOK60769.1"
                     /translation="RESMQFAYQSVKANKLRTFLSLFGITIGIFAIISVFTMIDSLEK
                     NVRDSLQALGKKVVYVQKWPWGGNEEYPWWKYLNRPEPTLSDLEEIRRKSLLTDAACF
                     NVAYNKQLKYNGNNIPDATIYGISTDFDKIRTFDLSEGRFFNQFEINSGRPYAIIGSD
                     IAKDLFQGANPLDKTIKIGGQKVTVIGVFAKEGKSAIGENSLDNMVIIPLNLARTMVD
                     LRNSGPWIMVKAKDDTSVEDLIDELRGILRAHHRLKPLDEDDFALNQIDLLKDSLDSI
                     FASINFAGGFIAFFSIIVGGFGIANIMFVSVKERTSQIGVQKALGAKRYFILIQFLYE
                     AVLLAVAGGVIGLLLIFIGTLAVTHGLDFNVSLTLSNIIRGLLISAVIGIVSGFVPAW
                     VASRLSPVEAINTKA"
     gene            complement(35564..36334)
                     /gene="rsmA"
                     /locus_tag="PK049_04080"
     CDS             complement(35564..36334)
                     /gene="rsmA"
                     /locus_tag="PK049_04080"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOK60770.1"
                     /translation="MPEVRPKKHLGQHFLNDKRVAQRITESLRADGCDAVLEIGPGMG
                     VLTFDLYQRFGSRLVAVEVDSESVSYLKRNLPDLNVVEADFLQFDVKELLKGKFAVIG
                     NLPYNISSQIFFRVIEQRDFVPEVVAMVQREVAQRIAEKPGSRTYGILSVFLQAFYNI
                     EYLFTVSEGCFTPPPKVKSAVIRLTRNSVNDLGCNYSVFERVVKAAFNQRRKTLRNSI
                     RSAFNGIEISDKYGTKRAEQLSVDNFVELTLEVERLLG"
     gene            complement(36336..37562)
                     /locus_tag="PK049_04085"
     CDS             complement(36336..37562)
                     /locus_tag="PK049_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60771.1"
                     /translation="MPINFFSKKIIFHLHASILVFIATACSNHYDRVEITIKVPHWTE
                     RWVYFRVPDFGSNFADSMLLDPKGEGSIVFNTKEPQVIAITTDKKDYPLIVSAKPGEK
                     ITLTYSNSWQADGSPETIQVVNFQNDINKVSQTLKNLQVKIDAIDSTEVLVKDSLIKV
                     YNQLKDSLKNGLQNDAYMFVASNPFELTSVFIVNATLDSKQLLPYTSYSKLYRKVDSC
                     LTLLYPKKQVVFDYKKTIGYHRINDSISQIENSLRPGMVVPPLQFQTFDGKYISIPGI
                     WAKWILLCFWDKESIGNSKNLKNLYSTYKPLGFEVVSFGVKMDSLMLNNLAKSDTLGW
                     YQVSLNDTYSINKLQLYGIARLPGSLLIDKSGKVIAKHLTVNALGLKLDSLTRVSKPK
                     PVLRPRIDSVLTNTPQ"
     gene            37713..39668
                     /locus_tag="PK049_04090"
     CDS             37713..39668
                     /locus_tag="PK049_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HOK60772.1"
                     /translation="MKYLSFDKEQLVNLEYSLNREILSTNRAGGYFSTTIILCNTRKY
                     HGLMILPIDEFDGENHVLLSCVDETVIQHGQTFNLGIHRYPGIYEPRGHKYIIDFSYE
                     PTPTLTYQVGGVIFKKELLFVHNEEQFMIRYTLVDAHSSTHLRIKPFLAFRNVHNVSK
                     ANMWANTKFQPCANGVKYKLYTGFPTLHLQLNKKNEFVACPDWYYNIEYMEELKRGYE
                     GHEDLFVPGYFEIPIKKGESVIFSAATEEQTPSTLLKKFNAEILRRPAKDSYKACMEN
                     SARQFIVHKGKSWDIIAGYPWFGSWGRDSFISLPGLTLGIDDPKMCKTLLDSLSAKLK
                     NGLFPNMGNATSQAFNSVDAPLWYFWAIQQYYLYTNDHKEVWHDYAKKMKTILKGFRD
                     GLPFNIKMHDNGLIWAGERGYALTWMDAVVYGKPVTPRIGYPVEINALWYNAIMFTID
                     LATKNKDYRFVKEWLPIAERAKASFNNLFWLAEKGYLADFVDEDGPNTDIRPNQIFAI
                     SLPYTPLDEDKMPSVLKIVEKELYTPKGLRTLSPNNPHYKGRYEGDQPTRDSAYHQGA
                     AWPWLLGAYVEAHFRLNPEHAINVAENILESFQEDISIHGICTISEVYDGDPPQRPNG
                     CISQAWSVAELIRIYQMVEKHKSGKGK"
     gene            39670..>40829
                     /locus_tag="PK049_04095"
     CDS             39670..>40829
                     /locus_tag="PK049_04095"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014775172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOK60773.1"
                     /translation="MRVLMFGWEFPPHITGGLGTACYGLTRALAKQGTEIIFVVPKAY
                     GDEDQRRVRLVSAEDIEVSHSSKNLEEFWKHIKYLEISSNIVPYVDPEEFSKIVEYAE
                     NESVEYNINTFKRKFKFTGKYGPNLMTEVTRYAITAASVAWRETFDVIHAHDWLTYPA
                     GIAAKMVSGKPLVVHVHATEFDRSGENVNQAVYDIERRGMEVADMVVTVSNLTRNIVI
                     NRYGIPEHKVVTVHNAVHFKGDIEQEFERGVDEKVVTFLGRVTFQKGPDYFVEAANKV
                     LKRTQNVRFVMAGSGDMLNRMIRRVAQLGIAPRFHFTGFLKGNEVNQMFQLSDVYVMP
                     SVSEPFGISPLEAMRSSVPVIISKQSGVAEVLKYAIKVDFWDIDALADSIYGL"
BASE COUNT        12425 a         8877 c         8403 g        11024 t
ORIGIN      
        1 gatgacccct ctaaatctcc cctgagaggg gagactttag ccctgcaata aggtctccta
       61 attgcttccc cctctcctgt caggagaggg gtggggtgag gtcgtttttt acgaaaaacg
      121 aacaacgagt tactagcaac gcttccagcc ccgcaagggc gtaatatttg taacactatg
      181 attattccca catgtatagc gatgcacgta atacattttc caaagagcaa cactgaaact
      241 ttgcatcgca acagaacagc atggctaaga gcagcttcca aaaaaacaac aaggattaat
      301 gctaaagaga tgcttcggcg aagtatttcc ctacaaatcg ggactcagca cgacggggag
      361 ctatttcggc agtaattatc ccgtaaattg gaaatcaaaa tgacaaaaat ccctaattaa
      421 acggtattgg cggtattttt gataaatcaa aatattgaag gggtatgccc cttttgaaga
      481 tcagcgctaa attgtatttt tgtagattaa gttaacaaat tggttcgggt aacgttaaat
      541 ggtaaaagtt catttacatg gataatagaa aacttatttt aataggattg gttgtaactg
      601 ccgtatgctt ttttgtttta atgctatcgt tcattccaag gcctaattcg ggatttgaga
      661 ggtataaatc gttaaaccct tggagcgact cggtttacca atcgtcgcta atttttgcaa
      721 tccccatacc tgagaatttg agttttgccg gcgaagaagt tccccttcag tatttcgatg
      781 ttcgcgaatc gcttgaccgt gaactacatg ttaacacctt ctggcactcg caaacggttc
      841 ttctgctaaa gcgagcaaac cgctacttcc caattataga gccaatactc aaaaaaaaca
      901 atatacccga cgattttaaa taccttgctg ttgcagagag tggccttacc caggcagtat
      961 caccttccaa agcagttgga ttctggcaaa tacttgaggg tacagctaaa gaatacgggc
     1021 ttgagattaa taaagaggtg gatgagcgat accatattga aaagtcaact gaggtagcct
     1081 gccagtactt taagaaagct tatgagaagt atggtacatg gaccatggca gcagcatcgt
     1141 acaactttgg aatgaacggt attacaaagc aaattgaccg gcaggatagt aattcgtatt
     1201 acgatatggt tttaggcgac gaaaccggca ggtatgtgta ccgtatatta gcgcttaagg
     1261 tgatttttga gaaccccaag cagtacggtt tctttctgga tagtactgac ctttatccac
     1321 cacttgagta cactgaggtt aaagttgaca gcaccataac cagcattgct agttttgcaa
     1381 ggcagtacgg cgttaactat aaactcatca aactctttaa tccctggctt agggaaaatt
     1441 acttgccagg caagcaaggc aaatcgtacc ttataaagat accacgtaaa ggtttccgtg
     1501 aggaggcata taaataggtt ttagcatgtt tcaggatatt gataaatcgg ccttaaccga
     1561 tgagggcgaa attgttgttg agggtgcacg cgtgcataac ctgcggaatg ttgatgttac
     1621 aattccacgg caaaagctga cggtaattac cggcctgagc ggcagcggta aatcatcatt
     1681 agcatttgat accatatatg ctgagggtca gcggcgctac atggaaacca tgtcggccta
     1741 tgcccggcaa ttcctgggtg ctcttgagcg acctgatgtt gataagattt cagggcttag
     1801 ccctgtgatt tccattgagc agaaaaccac cagccaaaat ccccgctcca ctgtggggac
     1861 aattacagaa atctacgatt tcctccgtct actttacgcc agagcgtcaa cagcctactc
     1921 ataccaaacc ggggaggaaa tggttcgtta taccgatgag caaatcatta acctgataaa
     1981 cgaacggttt actggtaaac gaaccgccat actggcgcct gtggttcgtg gacgtaaggg
     2041 gcactacaag gaactttttg accagctaag gcgcaagggt tacctcaacg cccgtatcga
     2101 tggggaaata acggagctac gaccggccat gaagctcgac cgttacaaaa cccactacat
     2161 tgaacttctt gttgacaaat tccgtgtagg cgagggcgat ttaaaaaggt taagccagtc
     2221 ggtggctaca gcaatgaaca ttggcaaggg aatcatcatg gtgcttgata tggataacgg
     2281 tgaataccgg tactttagcc gcaacctgat gtgtcctaca actggcattt cgtacgatga
     2341 gcctgagcca tacacattta gctttaactc tcccaaaggg gcatgtacac actgtaatgg
     2401 gttgggttac ataaaccaag ccgatattga acgaattatc cccgacccac agctgagcat
     2461 taaacgaggt ggtattcaac ccttgggacc atataaaaac tcactgatat tctggcaact
     2521 ggaagcaatt gcctcaaagt acgatttttc cctttctgac cccattggac aaatccctga
     2581 tgaggcgcta aacgtgattc tttacggttc cgaagaacag tttaagctaa aacatccaag
     2641 cattggggta aacagctcat acttcctgag ctttgaaggg gtaatcagct acatccaaaa
     2701 ccatcaaacc aacgatgagc tgaatggcaa gggcgaccgg tgggcaaacc agttcatcaa
     2761 acaggtaacc tgcccaacct gcaagggaac aaggttgaaa gaagaatcgc tatggttcaa
     2821 ggttgatggt aaaagcatag ccgatgtttc agccatggac attgaaacac tctggcattg
     2881 gctacagggt cttgagaatc gtcttggcag caggcaaaag gcaatagcca ccgaaatact
     2941 taaagagatc agggagcgcg tacgcttttt gctcgacgta ggattgggtt acctttcgtt
     3001 aaaccgcggt tcttcaaccc tatcgggtgg cgagagccaa cgcatcaggc tggctaccca
     3061 aattggttca aaactggtta atgtgcttta catactcgat gagccaagta ttgggcttca
     3121 tcagcgcgat aacatcaagc tcataaactc gcttaagcag ctgcgcgatg caggcaactc
     3181 cgtgatagtg gttgaacacg atgaggagat gatacgctca gccgattacg tagtagatat
     3241 aggcccacgc gccggcaggc atggcgggaa aatagttgca acaggcactc cctcggaaat
     3301 actgatgtcc aactcgctta ccgctcagta cctcaatggt caacttaaaa ttcctattcc
     3361 taaagagcgg cgcaagggta acggaaaatt cctaaccgta aagggagcac gaggcaataa
     3421 cctgaaaaat atcaccgtga aatttccttt aggtacattt atctgtgtaa cgggcgtatc
     3481 gggtagcggt aaatcaacct taataaatga aacgctatac cctgccctca gccgccactt
     3541 gtaccgttcg tatgctcaac cgttggaaca cgatgcgata gaaggaattg aaaacattga
     3601 caaagtgatt gatgttgacc aatcgcccat tggacgcacg ccccgttcga acccagcaac
     3661 atatactaac gtatttaccg atattcgcaa gctctttgag ttaacccccg atgcaaaaat
     3721 aaggggatac aaggctggac gcttctcatt caacgttaag ggcggacgat gtgaaacctg
     3781 taagggcggt ggcgttcagg taatagaaat gaatttcctc cccgacgttt acgtaaagtg
     3841 tcccgactgt tttggacgtc ggtataaccg cgaaacgctt gaggtaaagt acaaaggtaa
     3901 aaatatcagc gaggtgctgg acatgaccat taaccaggcc gtggaattct ttgacagcat
     3961 accgcaaatc catcagaagt taaaaacgct ccaggacgtt ggactgggct atataacatt
     4021 ggggcaacaa tccactaccc tatcaggggg tgaagcccag cgtgtgaaac tagccacaga
     4081 attagccaaa cgcgataccg ggaaaaccct ctacattctg gacgagccca ctacaggatt
     4141 acactttgac gatgtacgca ttctgcttga ggtgctcaac aaacttgttg accgtggtaa
     4201 cacggttata gtaattgagc ataatttgga tgtaattaaa tcggccgact acattgttga
     4261 ccttggcccc gagggaggag cagcgggagg ccaagtggtt tgtactggca cccccgaaga
     4321 tattgcaaaa tcgggttgtg gttacacttc ggaatttctt accaaatact tattcaggga
     4381 gtagccattt tggaactttt accgacctaa gttcgttaaa taaaaagtta atgctagcgg
     4441 tatggataca ctaaaaattg agaaacgaat cgactcaccc atggtgctgg ccgatggtga
     4501 atcgggatac tttgaggtga atggcaaatc gttgcccgaa gatgccattg aattctataa
     4561 accccttgaa aaatacgccc aggagtacgt aaaatcacca aagcaaaaaa caaccattaa
     4621 cctaaaattg gagtacctca acacctcgtc gtcaaaaaaa ctactcgata tcataggtta
     4681 ctttgaagca ttgccatcgc aggggtacga ggtggtactt aactggtatc accgcgatga
     4741 ggaccaggac atgattgatg agggtattga gtttgcccac atgactagcc ttaaagttaa
     4801 ctttatagtg gagcaataga actaagtatg tttaggtttg ttcaatacta tgttttattg
     4861 attttgtttg catttgcaca ttttactgcg caatcgcaaa caaccccgct aggcagcagt
     4921 aaggaactcc aaaatagctt acagcaagcc aaggaacttg aaagtaatgg cgacctaaac
     4981 ggtgcacttt caatttacaa taaaaccgca accgcttact gggttaacgg aaagcttacc
     5041 gaggctcgcg acattttcct aaaagcagca tcggttgctg aaaagcttgg aaaccaaaat
     5101 gcactcaagg taatttacac caaccttggg atggtgtttg ttgacttgga acagtatagc
     5161 gaggccattg cgcagttcga aaaaagttta gccataaacc gctcacagaa aaataaagcg
     5221 gaaacaacgg caacccttat taacatagca aatgcctaca aagaattgga caagtaccaa
     5281 aaagcccttg attacgctga acaggccaat accattgcac ttgagattaa cgatgcaaag
     5341 ctactgagaa acacatactc gctcatggct gaaatacacg aggcacttgg aaattctgaa
     5401 aaatcgtcgg agtacttctc cctctataca gccttttcca gaaaaattca acgcgaggaa
     5461 cagctcaaaa aggaagcaga ggcacagaaa attgttgagg aagcaaaggg agaactccag
     5521 gtaataaaaa gtgaaaaaga gctcacagaa aaggaactca gcgaaaagca gcgagtactt
     5581 gaaacagtaa aggatagtct cgataaaatt gaacagctaa cgcaagagca aaagttacag
     5641 atagacctgc taaataagga gaatgcactg cgcgaggaaa aaatcaaaaa tcagattctg
     5701 gttcgcaaca tctttattgt aatcatagcg gctaccatag gcatgcttgg cctaataacc
     5761 cattactacc ttcaaaagaa acgctccaac gaggaacttg cacgccaaaa agccatggtt
     5821 gaggccaaaa gccaggaact gatgcaagcc ctccaacaga ttgagaaaca aaaccgcgat
     5881 ataaccagca gcattaacta cgcgcagcga atccagcaag ccttattacc cacatacgac
     5941 caattggtca gctacatacc caatgcattc atcatgttcc gtccgcggga gattgtaagt
     6001 ggcgattttt actggtttgc tgcctatggc ggccaaaata tagccgccag taagcaaaac
     6061 cgacacatga tagctttgcc caatatcccc aagaacgatt ttggttttct gattgcagca
     6121 gttgactgca caggtcacgg tgtgcccgga gcattcatgt ccatgattgg gctaaaccta
     6181 ctcgatgtaa ttgtacgcag cggattaacc caacccaacc taatactaaa tgagctgcac
     6241 aagcaggtac gttacctgct taaacaggaa aataacgata gccgcgatgg catggacatg
     6301 gcccttatac gaatcaaagg cgatggccga acaatagagt ttgccggagc caaaaatccc
     6361 attgtttaca taaccaacaa ccagtgtttc accattaaag gcgaccctgt acccattggc
     6421 ggtttacaaa aggaagaaaa acgagagttt accctacata ccatcactat cgattcccct
     6481 accaactttt acctgttttc cgatggatat attgaccagt ttggtggacc cgaaaacctt
     6541 aaatttacct ctaaacgatt cagggagatg ctatttgaga accataattt acctatggtg
     6601 gctcaacaag aaaaacttga aacagctctt gatgaatgga ttggctccga aaataaccaa
     6661 attgacgatg ttcttgtcat tgggcttcac ctaacaggta aaccttttaa aattgatagc
     6721 caaacttaac tgcaaattga taaaaacttt tatttttgtg aatacatcac attaaaccat
     6781 gagtaagcaa tattttgggg ttttagcgtt tctgtttctt tttacatcag cattggcaca
     6841 agaaaatgcc aacaatgtat atgtgaacaa caacggtgat gtttttgtaa aagccgatgc
     6901 gcctatattc tttttcatca gccccgagaa ccagccctcc cagaaagtgc tcattcccag
     6961 taccgacaag gccgctaacc cgatgtattt tgatggcgac ggtaaacact acctggtgta
     7021 tgaggggaag ggtgaaaagg tacgcttcct aatttgggcc gatggagtgg gtcctaaatc
     7081 aagcttaagg gttgaaaaag gacttctctt taaccacaat aacagggttt atgtggagga
     7141 cagggcatca tttactccca gtgcaatcga ttcaaaatcg ggtgtaaagc aatcatttat
     7201 tgccgttgat aaccaaccgt ttgcaccttt taatggaaat gttgatatag aacgaaccgg
     7261 cgagtacacc ataaaggttt actcaaccga caatgtaggc aacgttggcg atacagtcgt
     7321 ttataaaatt gttgcagccc ccgatgtaac ctttaaaatt gataatatct actttgaaac
     7381 cgcaagcgca cggattacag gaaactctat caacaacctg aaagaaatgg tagagatact
     7441 aaaaaactac cctgaactgc atgttgaaat taaagcccat gccgatagcc gcggttcaag
     7501 cgagtataac cttgaattat cgcaacgccg ggcccagtca gtggtaaact acctcacatc
     7561 gcgaggaata gaaggttacc gtttaaaaga taaaggctat ggcgataccc aaccggttaa
     7621 cgagtgtgtt aagggaataa aatgccccga tagcaagctc cgtgaaaacc gtcgcgtgga
     7681 attccgcttt tacttaccaa aaaaataacc atgagtatga ataggtttgc tcaaatactt
     7741 cttgtgaccc taaccggctt tacttttggt agtttcgccc aagacgccga gaaaaaaaca
     7801 ttttatattg attcaaccgg gaatctttac gttccgctca gccaacctgt tcaaatatac
     7861 atgggcacag aacccgatgg ctccaatgca gtattgctaa aaagtatcaa taaaatatca
     7921 gcccctttaa cctggaatgg ttcgggtcca caactaatgt cgcaccttga cctttacaaa
     7981 ggtcggaaaa tcctatttga actttacgcc gacggtatcc caccccaaac atccataaac
     8041 cccaagagtc agaatattct agagcaaaaa gatgcgatta ttatccgggg gggctcaatt
     8101 attgaactca acgctaccga tgaagcggcc ggtgttgata aaatactcta ctcaattaat
     8161 ggaagcagtt ttgctccgta caacacccct attaacctca ccgatgatgg cgaatacgag
     8221 attaaggtat actcaaccga taagcttggt aacagtgagc cagtagtatc ccgaaaaatt
     8281 attgtggatg ccaccccacc cttaaccgaa atctcattta agggtgataa gtacgaagac
     8341 attttttcag gtagggctgc gcttactctt acagctagcg acaggtttgg tgtagcaggc
     8401 actaaggtta tgattgattc atcgggcaag tggactgatt ataaacaacc catacaaact
     8461 gctaacctgg ctgagggata ccatacccta acctggtact caaccgatag agcagggaat
     8521 attgaagagc aaaagaacta cagattcttt gttgataaaa ccccacccat tgtagttgag
     8581 gaaattgaag ggaactcata catgattggc acccgtgagt tctcatcggg acgtagccgt
     8641 ttaaaagtaa tggccgttga caacaaagca ggggtaaagg aaatttacta ctccctgaac
     8701 aacagggagt tcaagctcta cgaaaaaccg gtaatgcttg ccgatatctt gggtacggta
     8761 aaactaaaaa cttacgctat cgatagggta aataaccgca gtaactccga ggcaaacgct
     8821 cagagcttca ccatgcccac aatcgatata acagggcccg aacttggcta tcgcctaaat
     8881 ggccctaaac ttgtattgcg cgacacgctt tggatagggc ctgagaccaa aattgagctc
     8941 cttgcaaccg atagggaagc tggtgttaac cgcattgaat atgccctgaa tcaaggacaa
     9001 aataaacaat accttgagcc attcgtttta accgacgatg gattctataa acttgagttt
     9061 accggttacg acaacgtgga aaacgtaaac gtatcgggct ttacctttgc ggttgatacc
     9121 caggcccctg aaatttactg gcatttcagc gttaaaccca ttggttttga gcaggttgaa
     9181 agccaaaccc ttccggttta tccggataat gttagaatat atattggcga gtccgataac
     9241 ctcacagcca caaatactat tcagtattcc attaatggat caaaaaatca aaattacaac
     9301 acacccataa caaacctcaa ggtgggcatt aatgagttag ccatttttgc taccgatgcg
     9361 ttgaacaaca cccgtaagga aactgtaaga ttcataataa gatgataaac tcctaaaaga
     9421 gtataaaaaa ggctgcccga tgggcagcct ttttagttaa cgctcgtttt catcgatttt
     9481 caaatgctgc tcattattct tgtaaatctc atcaacgcta tcgttctcct cagggagttc
     9541 aacctgcttt tcgttcgata ggtcgatatt caccacaatg gcaggtactt ttaccgatgt
     9601 aacccaaaca tttccattcc ggtaaacata aacctcgtga acattatccc agtaaaaatt
     9661 atacttaggg aagtaaaccc aacggcgata atacaccttt ttaggtgccc aacgcggatg
     9721 ataaaccaca acctttttgg ctcgtggccc tttatgctta accactacag tcttgtggcc
     9781 tggttttact ctaaccacat tttgtgcatt ggctgcatac gatgccaaaa taaaggcaac
     9841 tgcggttaag gtcaaaattt tagctatcct tttcatatgt gtaagtgttt agtttctgga
     9901 tataataaaa cctaaactta aaatacccta ccaaaaaatc agtataaggt agatactcat
     9961 tatggggaaa aaatatacta ttattttacc aactatttac atactgatag ctcagcatta
    10021 acccccttgc gatagttgaa ttctaatttt gtggcaaaac accgaaggaa tgaatctgag
    10081 aaactacacc attcgaacac tcttgaaggt aacggcattt gtggtaatct ttacgctgct
    10141 tgtttttacc ttgatatttt tgagccagca acgtaactca caacgtcaaa gagaagcact
    10201 aatttcactt gagcaccttt accaacaaaa tatatcatta aagcagatta aggataactt
    10261 tatttataga gatacccgat cggaacagct ttacaagttg gggtactcaa actctaccct
    10321 aaagcacgat agtctgatca gggtatgtaa agaaacgctc aaacaaattg gaaatcatta
    10381 caattataat cagctagacc aaattggtca gcaactaagc acctataata aaaagtttta
    10441 cggcgttaag gagttggttc aaaaacgtgg ctttaaggat tttggaatag aaggcgaact
    10501 aagggcaaaa atacatgccg ttgagagtac ggttgaacaa ttcatataaa aaacgagccg
    10561 aaagaaaata ttcggctcgt ttttttagag ttggactggt tttcaactta aaccatcttc
    10621 tcgatattcc atacaaaggc atggatacct tgctcctcag caactttgtt tatttgattt
    10681 atatacgata ggagtttatc cactttatca tcgtcaacta tggttagcat gccggagttc
    10741 atggatggcc aagtgtgtgt acctaaatgt ggttcacctt tttggctacc agcaccaagc
    10801 atgtcggccc atttgctgaa tccttttact ccagcaatct gtaaagcact atcaacctta
    10861 tcggatagag cctgattgta tgcaatgaaa atcgctttca tatcgttagt attgagaagt
    10921 taatcgtcaa aagatatttc actatcagaa gcttggagtt tcttccgttc gcgtttaata
    10981 cgagcattgg caaaaagggc ataaattatg ggcacaataa ccagcgatat caacatggaa
    11041 aagacaagtc caccaacaat tgatatcccc agaggtcgcc atatttcaga accctcactt
    11101 tttgaaagag ccataggaag catacccagc aaggtggtaa acgacgtcat cagaatagga
    11161 cgtaaacgcg attttccacc ctccagtata gcttgggaaa ttgttctacc tctactaact
    11221 aacaggttga tgaaatcaac cagcacaatg gaatttttaa cggcaatacc cactaacatc
    11281 acaatggcaa tacccgatat catgttcagg gtagtaccgg ttacataaag ggaaatgaac
    11341 acccctgtaa gagcaaaggg aaccgaaaac atgatgatga atggttcacg cagcgattca
    11401 aactgtgctg ccataaccaa gtaggttaac accattatca gcacaaaaag cagcttaaga
    11461 tcttgaaagg tatcggccat gtccttagca gcaccgccaa tttcaacaat cacgttgtcg
    11521 ggtatggtca aggtagccag cttagcattg ataatacttg aagcggttcc caaatcgata
    11581 cccgaaagtg cactaaccac ctttactaca cggatcttat cttcatgttc aaggttaggg
    11641 gttgtgtaaa cctgtttaat ttgggccaca tcacctaccc gcaccatatt acccatggga
    11701 cctttaaagg taatattctc aatatcggac accgaggtga ggttggcatc gttgtacttg
    11761 agaattacat cgtactcggt accctcttcc ctatacttgg ttgctgttag cccatatatc
    11821 ctgttacgta ctgctgaggc tagcatggaa gtgttaagcc caagctgagc cagcttctcc
    11881 cgatcgggga taacctgaag ttccatacga tccttatcgc gactaatcga tatgtcacgg
    11941 aatccaattt cggcttccag agtgccggct atttgctcag caatgtaatt ggtttggttg
    12001 atatcgtaac caagtatttt aacctcaaga ttactacctc ctcccattcc taaagttatc
    12061 ctagaattac ggttccctcc tgggtcaaca tacgacgatg ttacctgggt gtaacccgtt
    12121 atatctttac gcaggctatc ggctatttcg aaaatgcttc tagtacgatc atcagcactg
    12181 gtgagtatta tgctgtaatt aatgatatat gaagctgact tttggaatat actcattaac
    12241 cttccccctt catcggcacc aatacttgtt gatatcaatg tgacttcggg atattttttc
    12301 ttaacctttt gttcaaaatc acgtgcaagc gctttggcct catcaatccg aataccctgg
    12361 ggtatctcca tatttacgtt gatgcgaccg ttatcctgcg aaggaaggaa ctctgtacca
    12421 actacaccgg ccaatcctaa acttccaata aaaaccaacc cggcaataat tagtgtcaga
    12481 gccttgtgtt ttaaagtcca agccaatgat tttacataaa tggtatcaaa aaagccccag
    12541 aaacggttaa cggattccaa gaacctgctg cgatgatgct tatagtacga acgcccctta
    12601 agcattcgag acgaaagcat tggggttaac gagagtgcag ctaatgtgga aagcgaaaca
    12661 gttatggtaa caatccatcc cagctggcgg aagaaaacac caattaagcc acctataagg
    12721 gttaacggca tgaataccgc aacaataaca agggttgtag caataactgc caaacctact
    12781 tcgcgggtgg caaaaaccgc tgcctccatg gggctactac cccgttcaat gtgagtggtg
    12841 atattctcca gcaccacaat ggcatcgtca acaaccatac ctattgcaat agacaaagcc
    12901 gataacgata caatgtttat ggaattgcct gaaataaaca ggtagatgaa agccccaaca
    12961 agcgaaatag gaatggtgag cataataatg aaagtggaac gccaacgacc gaggaaaaga
    13021 attacaacca gggtaataaa tacaaaggcg tatataagcg tttcggaaag gttattgatt
    13081 gaattttgaa taaattccga tgaatcgata attttaacca ccttcacatc cgaagggaga
    13141 tctttttgta attcatccat actttcccta accaatcggg caattttaac ggtgttggct
    13201 ccgctttgct tctgaaccat aagcctaaca cccaaactcc catctattct ttcatcaacc
    13261 agcacacttt ttagggtatc cttaacagta gcaatgtcct tcatgtaaac cagcttgcca
    13321 ccattgttgg ccacaacaag gctcttaacc acatcgctgt ttctaaactc cccgtttgta
    13381 cgaaccggaa actccattct accaacttcg atacgaccgg caggtagcga gatattctca
    13441 gccccaataa cagctcctac ctgttcaagg gtaatattgt aggcatcaag cttacggggg
    13501 tcaacagtta catcaataat tcgaataggt ttacctataa tggtaactga tcccacacca
    13561 tcaatacgac tcagcttttc aatgattttc tcatcaatca atttttcgag agctgcataa
    13621 ctcctatcgg cctgtattcc gtatataaca acaggtacca tcgatgagct gaacttgaaa
    13681 attgatggtt tctcacatcc atcggggagt gatttttcgg caatgcttac cgcatcgcgt
    13741 acgtcgttac ttgcctcatc gaggttaata ccatactcaa actcgcaggt aacaaacgat
    13801 atgttatcct tgctcgatga ggttaccttt ttgaggtttt gcaaagcgtt aagctgattt
    13861 tcaattggtt tggttacatt tacttcaata tcctcagcac tagctccagg atagaatgta
    13921 attacactaa ttgaagggat ttccatctcg ggcaataagt caataggtat gtaaaatatt
    13981 gaataaatgc ccattacgat taccccaacg aaaatcatca gggtagatat cggatttttt
    14041 acggctgaac tgtaaagatt catactaagt cgaattaaga attacttttt aaccacgggt
    14101 tgaccatcga aaacaatggt ttgtccctca acaataatct cctcaccggc agataggcct
    14161 ttaataattt ctactttatc gtcgaacaca tcaccttgct ctatgttcct acggatggct
    14221 ttcccgttat cgtaaacaaa cacatatcgt tcattggttc ccgattgttt tagaaccaca
    14281 aacgctggca caagcagtgc atcacccaca cccacattta tgtaggcagt agcaaacatt
    14341 cctggcctca gcatcttatc ggggtttggt acaaccacct caatggtaaa ggttttggtc
    14401 atattatcga tggtagggta aactttctct actgtacctg taaactcctt atcggggtaa
    14461 agttcggttt taacggtaac cttcataccc ggctttactg catttaaata ggttgatgat
    14521 accccaacca gaagtttaag cttatcgagc tgcatgattg ataccactgc aggcttccct
    14581 acacccggag caggcgacat ggtaaatagc tccccatcgt taatgtattt accagtaata
    14641 attccgttaa taggcgattt taacgaggta ttatcgtcaa gaaactcaag gttcgttttg
    14701 gcaatatcgt actgcatttt tacagcatcg tactgctgtt gtggtattgc tcctactttt
    14761 agcaacgaat ctacacgttg caggtcaatt ttaagttttt caaactggat tttagcttgc
    14821 tgataggttg tgcgatcgag catggctaca acatcgccgg cttttaccct atcgcccaca
    14881 tcaaccaaaa ccttttctac ccttccggca gttgcaggcg ccaggtgatt ttccttgtaa
    14941 gcctgaatgg ttgcaggata cctaaactgg cgattgatgc taacgggttg tacaacctga
    15001 gtaaccactt ttacttcggt agcagtggtg gcaagttgtt tttcatctgc cctatttccc
    15061 tgacacgagg caagtaaaac tactgcagag gcaagcgtaa ttgctttaaa ggtattaatc
    15121 gttctcatag taatagtgta ttttgcgtta tattttttta cttttaaaat gattcaccga
    15181 taattttttc gtaatcggtt ttagccacaa ggtactccaa ctgtgcctgg attagattta
    15241 acttagcctg aagcattgca ttttcggcat cgttcaactc caaaagcgtt gcttgaccag
    15301 tattgtagcg ggtttgagca atagtatatc ctttttccgc caggtcaaca ttaccctttg
    15361 cagcattaac tttttttacg gcagtattca cagcatttgc agcattctga agttgaactt
    15421 caaggttatt cctagtgaat tgggtttgca aatcgagctg ctgctggctt atacgaacct
    15481 ggttaagctg cttacgaacc gaaaaaccgc taaaaattgg aatattaagc tgtaagccaa
    15541 ccatcgatgt agtaacccaa ttgtaattgc taaaattgaa atcgttggct tgggtttggt
    15601 actgatagtt accaatagct accagagaag gataatttga gcccttcact atcctctcct
    15661 gaatggtaag catttggcgg gttaatgcca actgcttgag tgtagaatta cggccaaacc
    15721 agctggaatc cgctactacc aactcttcga aattatagct taaaagttca ttctcatcca
    15781 gcttaaccgg agtattgaca tcgatattga gtaaaacctt aagtacattc aaaaggttgc
    15841 tgtatgcatt ggcagtttga accaaattgg gataaatatt atcaacctga accttaattc
    15901 ggataaggtc ataattcgat accatcccgt tagcattaag cttttggata ttactaagat
    15961 tggcaagggc attctggtag ctttgtttaa ttacatcatt ggttttgcaa agcaacaaaa
    16021 cattgtagta agtggttttg atactggcag ccaggtttat tttgttctcc ctgacctttt
    16081 cagcgcttat ttcagcatcc ttacgcccca gcttaatcga ttcaaaaaca ttgtaaatga
    16141 atactggcat cgaaccgctt actccaccgg tgtagctgtt atcggaacca actttaagag
    16201 taggcccaaa gggtgaacct tcaggcatga atataaccgg taacttgaga tttcgggtgt
    16261 aacttgccga tgccttaatg tttggataaa gggcagccat atactctttt accttttcct
    16321 gtttcttttg cagttcaagt tcagttacct tatgcattag gttttgctga atagcaaggt
    16381 caaccgcctg cttaacagtt aaatgcaatg tgtcgggctt ttgctgccca agggctaggc
    16441 ttgttgaaag gacaaagcct gcaagagaaa aaattgttat tagcttacgc atactgttaa
    16501 tgtttaaagt tcattatcga atttgtttgt gtttttcgta gtaataatca accagttcaa
    16561 ttccttttga agtggcaagc ccgcgaataa aatttacggc catgtgttca aaaacttcgg
    16621 ccaaaggaaa atcgtttaag gagttggagc ccggttcaaa tccgctacga aggagttttg
    16681 taaattcaaa aaccacgctg gccacgattt gtggatttac ttcagctcta aaaagattat
    16741 cggccttacc cttttcaata aactcaacca ttctctcctt caagtaatgg tcaaactctc
    16801 ctgcttgctt acaaatggtt ttggggtaat ttttttttaa atcgtacatg aaaagtgggc
    16861 taacattgcg taacttattg atatgcgacc acatgaacat gatataggtc tcaatgtaat
    16921 gctcactttt gttcaaaatg ctttggacca tggcctgcat actttcattc tcgagtaaaa
    16981 cacaggcctc aactatttca tccttattcc cgaaattctc gtagatggtt cgcttggaca
    17041 ttcccaagtg ttcagctagc atatccatgg taatcccacg aattcccatt cgcttgaata
    17101 gttccagtgc cccttctact atctgctcct taaccgccat aaaactattt aaactatttt
    17161 agttttctga tggcaaaagt aaaacagaaa actaaataaa ggagaatagt tttgttaatt
    17221 tacctgaaaa catgtttttg ataattcaaa gagttaaagt gtcgattagc aaaattcttg
    17281 tatttttgca agttgcaaaa aatcagataa aacaatggct ttatacgaag aacttagcga
    17341 acaggaaata tttcgccgac aagcgcttga tgaattacga agtctaggga ttgaacccta
    17401 ccctgccgcc atgtatcccg taacccattc cactgccgat atcctatcgg attttgagaa
    17461 catgagccaa aaccgggcag aggtttatat tgcgggtaga attatggctc gccgtatcat
    17521 gggaagcgca tccttttttg aattacagga cgagaagggt aaaatacagg tttacgtgaa
    17581 acgcgatgat gtttgccctg gcgaagataa aaccctgtat aataccgtgt tcaaaaaatt
    17641 actcgatatt ggcgatatag tgggtataaa gggatttgtt ttccgaaccc aaatgggcga
    17701 ggagtcggta catgccaagg aacttaccgt gctggccaaa tcgttgcgtc cgctacccat
    17761 tgttaaggaa aaggacggaa aagtatttga tgctgttacc gaccccgagt tccgctaccg
    17821 ccaacgctat gtcgatttaa ttgtaaaccc cggtgtgcgc gatattttcc gtaaacgggc
    17881 taccataata aacaccatgc gagagttttt caactccaag ggataccttg aggttgaaac
    17941 gccaatcctc cagcccatac caggtggtgc agcagcccgt ccgtttgtta cccaccataa
    18001 tgcactcgat atccctcttt acctacgaat tgccaacgag ctttacctga aaaggctaat
    18061 tgttggtggg tttaacgggg tatatgagtt tgccaaggac ttccgcaacg agggcatgga
    18121 tcgcacccat aaccccgaat ttaccgttat ggaaatttac gtggcttaca aggactactt
    18181 ctggatgatg gacttcaccg aggagatgct tgagcgtgtt gcccttgcca tacatggcac
    18241 taccaaggta acctttggcg ataaggagat tgactttaag cgcccattcc gccgcatgac
    18301 catgaccgat gccattaagc agtatgccgg tgttgatatt acgggcaaat ccgaagatga
    18361 actccgtgct atttgcaagc aaatggatat tgaaattgac gataccatgg gtaaggggaa
    18421 actgattgat gccatctttg gcgaaaagtg cgaggagcat cttgttcagc caacctttat
    18481 catggactac cccattgaga tgtcgccgct ttgcaagcgt caccggagca accctgagct
    18541 aaccgagcgc tttgaactat ttgttgctgg caaggagctt tgcaatgcct acagcgagct
    18601 gaacgacccc atcgatcagc tggagcgttt ccaggagcag ctccgcctaa gcgaacgcgg
    18661 cgacgatgag gccatgtata tcgatatgga ctttgttaga gccctggagt atggtatgcc
    18721 aacctgctcg ggaatgggta ttggtatcga ccgcctaacg atgttcatga ccaaccagcc
    18781 atccatacag gatgtactat tcttcccaca aatgcgcccc gaaaagaaac cgcagaagga
    18841 cactgcagaa gcctacgccc aaaaaggcat tcccgccgac tgggttccag ttctgcaaaa
    18901 aatggggttt aacaccgtgg cctctctgaa cggagttgca gccgggaaac ttttcaacga
    18961 cctttgcggc tacaacaaaa agaataagtt aggtctgccc aaccccacca tggacgatgt
    19021 aaaacggtgg gtggagtagg ttattagaca atgaggtttg gaaaatccaa acctcatttt
    19081 attttacttt atcttttaaa ttaactaaat ttatcttcta tattctcctt ttaattcata
    19141 taaaacccta aacaatttag ttgtgcaaat tgaaattatt gtcaaaaaac ctgctaatta
    19201 caatttgggt gcatctctgg aggttttaat tgacttttta agtcaaattc aaaaatggga
    19261 taagggatcg aaacttataa ttaatttgca aaatattgac tttacttatc cattgacatt
    19321 acttccaatt gcatcatatt taaaatatct ctcatccctt tattatgaca ttgattttgt
    19381 ttacaacaaa aacactcaga aatacttaaa tgtaattaag tttccatatg gtctatcata
    19441 cgattacaat gaattaacta ttgaattgaa aaaatataaa aacaaaactt atttacctat
    19501 ttgttcaata ccttctgggt caaataattc aaaaattaga gaccaaatcc tttccctctt
    19561 tgaaactata cttgtaagtc agactaacat caagggtcaa cttaaaattg ctgttagcta
    19621 tataattaat gaaacgattg acaatatcgt tgagcacgcc aacgtgtcaa atggttggat
    19681 tatggctcaa aactatccaa aaaaaggttt tttggatgta tgcatcgcag atactggcga
    19741 tggtatactt gaatcttata aaaaaacaaa aatctttaat atttcaacag atgcacaagc
    19801 cataaataag gctatcaatg gcttaagcac aaaagaacaa ggtaatacaa gagggtatgg
    19861 tcttaaaact tcaaggaaaa tgttagtgga aggacttgga ggtatgttct tgctcttttc
    19921 aggaaattct ttctacattt ggaacactaa tattgaaaca attttctcag acattccata
    19981 taaatggaaa ggaacaattg ttgctgttag aataccccat gacataccct caaacttcaa
    20041 ttacacgaat taccttgaat aaataaatta caaaaattca aataaaaagc gctattcata
    20101 aacattttta acactttttc tttgtaaaaa gatgataatt tttttaattt tgtaatatga
    20161 aaaccaaagt cattcatatt agagaatatt tgccttcgat tatatcaaca aggaaggcta
    20221 taaaggaaat ccgtgaatca atttcctttg ataataactg taaatatatt tttgactttt
    20281 ctggtatttt ctttatttct cgttcttttg ccgatgagtt ttataaattt gttacttatt
    20341 cccatataga ttattcaata ctgaatgcta ataaaaacat tgaagaaatg cttaacgcag
    20401 tatcacggat ttctaaaaaa gatataaaaa ctgatactga tgaaattact tttacatatt
    20461 ttaaaaatat taacgaatta catagtttct tatcatcgtt ttaaatactt ccatttacaa
    20521 tatagattta aggcctagcc aactaggcct tttctatttc acgggggtca agggcatcgc
    20581 ggaggctatt gccaagcagg ttaaaggcca gtaccaaaag catcatggca aagccaggta
    20641 taagtgccaa gtaagcctta tccataatga tgtagccgta atggtcacga atcatagtac
    20701 cccacgaggg agctggaggt tgcacgccaa tccctaaaaa gcttaatccg gcttcaagca
    20761 gtatggccgt tgaaaagtta cttgccgaaa ctatgattac cggtgccatg atattgggaa
    20821 gtacatgcct gaaaatgata cgccacgggc cgtaacctag aaccttagcc gcctcaacat
    20881 actccttttc cctaacgctg agcacctgcc cgcgaacaac gcgagccata tccacccaca
    20941 tggttaagcc aactgcaacg aatatttgcc agaaaccctt tccaattacc atggttaagg
    21001 caattaccat taacagggta ggaactgacc aaaccacatt caccatccac attatagcat
    21061 cgtcaataat tccacgggca aaaccggcaa tggctcccag tacaatgcca attattaaag
    21121 caatacccac ggcaataaac cctacagaaa gcgaaatgcg cgcaccaata accaagcggc
    21181 ttaaaaggtc gcgaccaaaa cgatcggttc caagaagaaa cctacgcgtt acaaggtttt
    21241 gacttaatac cctttgttgg gcatcggcta cagccataat tgcatgctgc ccattgtaca
    21301 gaaatgcagt gattgtatcg ttctcaagta agtatttttt ttcggggttt aacctgaaga
    21361 ggatttcggg tagagttaat tcggcaacaa tggcatcgcc ttcgggaatc gggttgtact
    21421 cactggcata taccctaata ccatcaaagt agattgaatc aacaggtatc tcgttgtaat
    21481 cctgttcctg cccataaaac attcggccca gaatatttcg cttttttacc tcgtgctgct
    21541 tagcaacacg caggaatttg actttactgc caggcttaag cgttgccagc tgtaaatgct
    21601 gaccatttgc atatggcgaa ctatcgggta tgattaggta tccaagaaac gctataatgg
    21661 caatagaggc tataagcaat agtgatgcta cagccattcg gtcggcaagt aacctacgta
    21721 tggctattcg cattggggaa ccgcttcggg ctaactccgc tagctgttta tccttccttt
    21781 ccaagttgtt cctttaatta atcctagaat ggctgcaaat gttatataaa agggataaag
    21841 gatttgtaaa ggtataaaaa ccgccaatag gttttgctgt ttggttttaa aaaggtaata
    21901 tgccaagaaa ggtaaatcta ctattgcttt ggcagcaagg agtgaaattg gaaggttaaa
    21961 agtaattaca cccaaaactg ttagtattgc taaaagaata atccaaaaat tcagggtaaa
    22021 aaccaggaac gataatagta gcgttaggga atcggtatat gcggttgcct ttgatgccca
    22081 acgacttcgg cgcaaaacaa aaccctttat cccgccgcta aaacttgtgt aggcagccac
    22141 cctaggcgat gctaagaacc taaaccgtaa atcagtaatg gttttggcat gatgcagtag
    22201 aaacatatcg tcgcctgagg ctatatcggt gcgcaatacc ttagtatcta cattaacctt
    22261 gcttcgcctg aaagccaaat ttgccgatga ggctataacc ggtttgccaa cagcaaaact
    22321 tgccgctgaa acggccataa gcgataggta atcgagtgcc tcgaacatgg aaataatact
    22381 gcccttttca aactggggtt ttacctcacc ctgtaaaaaa tcagtactcc ccatgctttc
    22441 acgaatagag cttagccact gaggagtgtg ggtgcaatcc gcatcggtga atacaacagt
    22501 atcaaaactg gctgctgata gtccataaag taatgcctgt tttttacctg cctgcccatc
    22561 aggtaaaacc aaaggtttta aaggtaattg gcttttgcat gacattacta ggtcaaaacc
    22621 gctatcggtt gagccatcat caactaaaat agcttcccaa aactcatgcg gtaaatcctg
    22681 agcctcaagc gaagcaataa gtttaggtag attttcgcgc tcattcctga atgccactat
    22741 caccgaaatg ccattaacag tttgactggg ggctccattg gattccaacc agttattggt
    22801 caacaaacct tttagtaacc atagaatggc tatagtgtaa atagcaatca gtatggttga
    22861 gattataatc attgcttgct cataaaatca gcaattctat taccggctaa atctatctcc
    22921 tctaaaggtc gagccacatt aaagcgcaac cagtttacct ttgtgctgct aaaatgctcg
    22981 cccggaataa cagccacgcc agtagcatcg taaaggtcag aagcaaactc aaagccatcg
    23041 gtaaagggtt gaggcaactt agcccagata aaacaaccgc catcaatttt gggaaccgta
    23101 aagtttaaac ggttaagcat accacttaag ctgtcgaagg aactcttaac cctttgcctt
    23161 agctcatcca taaaagcttt accgtattgg aactgtgcaa ggtaaaccgc aagcgcttgt
    23221 tgcataacgc ttggtgcaca cagcccgata tagtcgtgaa tggccctaat ggccttggtg
    23281 tgactgggat gatggattac ataacccaca cgccatcctg ttatacacaa cgattttgaa
    23341 aaactgctaa caatgaataa cttttcggag atattatcca aaggaatata gggtttttgt
    23401 gaaaagtata gctcctcata aaccgaatcg aaaacaacaa aaaaatttag ctcgttagca
    23461 agttcgagaa gtttacaggt ttctgctttt gtccaaactt taccgtaggg gttgccgggt
    23521 gaactaatga atatcaactt taccttattt tcctcaatgc atcttttaag ttcgtcgaag
    23581 tcaacaaact cttcattaat caaagaaaag ggaacaaatt tttggttgaa gatttgaggc
    23641 agccgactgt agctttcgta agcagggtca aaggaaaggg ttaccagatt ttttccaagc
    23701 attcgaaaaa gataggtgta aaccaacgaa aggccttcgg ttgctccctg aactaccaga
    23761 agctcatcgg gttcaactcc atagctattc tcaattagcg ctctaagctg cagattacca
    23821 attccaggcg catactggtg atgctgcgaa ttagcaatat cttttaaaat attaatgagt
    23881 tcagtgggag gctgaaagcc tggaatgccc tgtgcaaggt tgatacctcc atttactttt
    23941 accaggttac tgaaatagct tatatatgaa ccatcgggtt ttgctctcat aaaccaagag
    24001 tttaagcaaa atatctacaa aaatgcaatt aaaaaagcat tagatagaca ttgggtaagc
    24061 caaaattgta aagtttggct aaatttgcat gtaaccaaaa tttaaacagc atgcgtttta
    24121 ggtattttgt ttcaatagtt ttaatagtgt ttttggcggg atgcagcagc aaagatgcgg
    24181 ttgtaattag cgtaaaagta aagagtcctg aaaaaactac acttttctta agtaaaattg
    24241 atttcaataa aaccataaag cttgactcgg ttaggatttc ggctggcgaa agtgtaaaaa
    24301 agtttaaggt aaaacagggc gaagaaccgg agttttactc ccttagcact agcaaagggc
    24361 ttgccattac cctattagct tcaaaaggtg aaaaaatcaa cgtaatatta gaccttaaca
    24421 atgccatcga ctatcaggtt tcgggttctg aggattctga aaaggttcgt tgggttacaa
    24481 gaacatttgc caagtcaaaa accgcagttg acgagctatc aaaacgatac ctgaacgccg
    24541 agagtaactc ggaacgtgaa ttaataaaca accaatttga gcaggaacgc aagcgggtaa
    24601 aggacgaact caccaaattt gtttgggaga accccatgaa caaagccagc ataatggcac
    24661 tataccaaaa atataacgac aacctgtaca tttttgacag ctccgacgat ttactgctaa
    24721 taaaaatggt tgctaccgcg tggaaagcgc tttacccgga atcgagctac accaaaggga
    24781 tgcttgagga tatcaagaat attgaaaatc gcattgccaa tgctaaaatg cagcaactta
    24841 tccagaatgc tgagatgcct attcctgacc taagcattcc tgataggaat gggcgtagca
    24901 ttaaactatc atcgctaaag ggtaaggtta tacttctcga cttttttacc agcgagaaca
    24961 ctgcaagttt gctggataat cgtgagctat tggaaattta taagaagtat aaaagcagag
    25021 ggtttgaggt ttaccagata tcgttcgatg ctacccgtga gggttggatt aactacattg
    25081 agaacgccgg attaccgtgg attagcgtaa gggaggatga tccatcggct tcaaatgccg
    25141 ctttattcta caacgttaga caactacctg ccaattatct tattggtaaa gatttcaata
    25201 tcattggaaa aaatctatac ggcacaaacc tgcaaaaggc cttggatagc tacctgaaca
    25261 agtaaaaaac attatgagcc agggtaaaaa gatatacttt gcatcggatc tgcatttggg
    25321 cttgcccaac ctggagcaga gcctaccccg tgagaagctt ttcaatacct ggctcgatac
    25381 cattagccac gatgctgagg aagtatacct tttaggcgac atttttgact tctggtttga
    25441 gtacaggagg gttgttccca aaggatatac gcgattcctg ggaaaaattg cccagcttac
    25501 cgatagcggc attcccgttc acttttttac tggcaaccac gatgtatgga tgttcgacta
    25561 ttttgaaaag gaactagggg ttcaaattca ccgcgaacca attgttcggg aactttatgg
    25621 tgcaaagttc ctgcttgccc atggcgatgg ccttggccct ggcgatcccg gctacaaact
    25681 gcttaaacga atctttacta gccctaccct gcaatggatg tttgccaaac ttcaccctaa
    25741 cttctcgtta tggtttggta accagtggag tgtaagcagt agatactcca aggaaataac
    25801 ccatgttttc aggggcgagg atgagcaaat aaccaagttc tcaaggcata aactccagga
    25861 cgaacatttt gattttttta tttatgggca ctggcattca cccataatat atccgctttc
    25921 agaaaaatcg aaccttgtag tgcttggcga ttggttagta agcaacacct acagtgtttg
    25981 ggatggcaag gagctaaagc ttatacgcta ccaaccaccc aaagccgatg aggttttggc
    26041 tactacaact ttcttataac aagctactta atgaattttt tggctcattt atacctttcg
    26101 gggagcaaca tcgatattcg gttgggcaat tttataggcg atcatgtaaa agggaaacaa
    26161 atccttgcct acccgcaggg tgttcaaact ggcataaagc ttcaccgcac aatcgatcag
    26221 gttactgata ggcacgaggc atggaaaagc tgtagggaga aattgcgccc cagctatggg
    26281 cgatggtcgg gtgttgctac agatgtgatg ttcgattaca tcctagctaa agaatggagt
    26341 acatactcaa acaggcctct taaaggcttt acaagaactt tctactttca gatgcttcag
    26401 cgctaccaca tgctaccccc agcagtaagg ggattcttac catttatgat tcaaagcaac
    26461 agactctact cctacagcac cgatgagggt gtaaccagag cccttgagat aatgggtaac
    26521 tacaccacaa tgaaagggaa cccaagcctt gctctgcaaa tagtgaagga aaactatatt
    26581 gcgttcagtg ctgggcttca ggaattgctt tacgcattaa tacaggttgc agaaaaagaa
    26641 tacggtatac ctattagtat gccccatagc gtgaagcatc gaaccgaagg agaataaata
    26701 ccaggattgt aaacgcccaa agcgacgagc ccccatagct aaagaacggc aggggaatcc
    26761 caataacagg aaccaaccca atggtcatgc caatattaac cgcagcgtgg aaaaacagga
    26821 tggaaactac tccatatccg tatatacggg caaaatgttc ggtttgacgc tcggcaattt
    26881 ttagtagcct taacaggaaa agcgctataa gcaaaaccac aacagtagaa cccaaaaagc
    26941 cccactcctc acccaccgtg caaaaaataa aatcggtgct ttgctcgggc acaaagttga
    27001 atttggtttg agtgcccgaa aggtaacctt taccccagaa acctccggag ccaatggcaa
    27061 tctttgattg gttaacattg tacccccacc ctaacggatc atcttcaata ccaagtaaat
    27121 cgttaatacg cttttggtga tggacatcta gcacacgctc aaaaacgtaa tcggccgaca
    27181 ttgaaaaacc tatggttgca ataagaaaga atgaaattgc ataaacctgc cttagcctgt
    27241 tcttgtaagc atacaaaaag cctaaaacag cactaataag tactgggata agaaagatca
    27301 atgcattaga tagccttaaa tggaatagtt ccgacgaaat gtagagcaac agacctattc
    27361 ctgctgcgcc aatggatatt ctgaacgcat cgcggtgctg gcgagtaaat actacaaaaa
    27421 gtgttactgc tacaacaaaa atgaaagtaa ttaaaacagg agtgctataa acgagagtta
    27481 aaatgaaaac aattaccaga aaaaccaggt acgataatat ccatgcaggc aagccctcac
    27541 ggtataaaac caaaatcaaa gctccaaaaa ctaaggccga gccagtatcg ttctgcaaaa
    27601 gtataatgcc aacaggcaac ccaataataa ctgccacctt aacgtacgac caaaaccctg
    27661 aaagtttaaa accatagcta ctcataagct ttgcaagagc aagggcagtg gcaatctttg
    27721 caaactcagc aggttgcaat ccgaatgaac ctatgggtat ccatgacttt gacgcattaa
    27781 ccacacggcc aaaaagtaac gttgaaatca gcgttatcag aataatccca taaaagatat
    27841 aagcaaatgc cggatagatt ctatcgttgg agtaaagaat aacaattgcc atgaggaatg
    27901 caaagaatat ccaaaccagc tgtttgccat agcgctggct gaaatcgagg atatttctgt
    27961 gctcatcgct atagacagcg gcataaatat tcagccatcc catcaatacg agaacaacgt
    28021 acaggatggt tgcaacccaa tcgaggtttc gtagtatgtt atttcgcctt tgcacgtcta
    28081 tctaataggt ttgcgtcaag tatatatcgt tccaaatcgg gacgggaaat agaatccttc
    28141 aggtacttct caatcatcaa acttgcaata ggtgcagccc atgtagcgcc aaagccagca
    28201 ttctcaatat aaaccgaaat tgcaattttg ggatcgtcct taggtgcaaa tgcaaagaaa
    28261 actgagtgat ccttaccatg agggttttgg gctgttccgg ttttaccgca aatacgaata
    28321 tcgggcaaag cagctattcg cgcagtgctt ccgctacccg gcgctccgtt tactgccaaa
    28381 tccattccct caataatgta atcaaagtac tttggctcaa ttgaggtgat atgcttttcc
    28441 cggtacaggg agtttaaggt atcgccctct attgatttta ccacatgggg tgcaatgtag
    28501 tatcccttgt tggcaattgt tgccacaagg tttgccattt gtaagggagt aacggaaagc
    28561 tcaccctgcc caatagcaag cgaaatgatg gtaagcgaat tccagcctcc ctttctaaag
    28621 tacttatcgt aaaatgaaac cgaaggtact atacccttta gctcatgcgg taaatcgata
    28681 ttcagcttgt tgccgtaacc aaacgattcc acatgccttt tccataccga gaatccattt
    28741 tcaaccgaac caaattccct tttatcgata atgttccgga aaacatggca gtaataggta
    28801 ttacacgaaa cctgtataga ttgtatcagg ttggtaggcg atggatggtt atggcaacca
    28861 acaccacgtc ccacggtgta tcctccatag cagctatatc gggtttcagg cgtaacaacc
    28921 ccttcctgta atccgattaa cgcatttacc accttgaatg ttgaaccggg cgggtatagc
    28981 gccatgattg aacggttgaa tattggcttt aacgaatcct gttggagaac cttgaagtta
    29041 gcagtccttt gccttccaac caacaggttg ggatcgtatg ttgggctcga tatcatagcc
    29101 agcacctcgc cggttttggg ttcaatggca acaatgctgc ctattttatt ttgcattaag
    29161 cgttcgccat actcctgaag ccttgcatca atagtactgg taatgttctt accgggtatt
    29221 gctaacgtat caaatttccc ctgctcgtat gaacccttga tacggttatg aacatcgacc
    29281 atgaagattt ttacaccctt tttcccacgt agaatatgtt cataggagcg ctcaataccg
    29341 cttataccaa tgtaatcgcc ctgttggtag tatgggttat tcttaatatc gcgatcatca
    29401 acctcaccta cataccctag cagatgcgcc gcaatgggtg gattatatga acgcaccgta
    29461 cggggttgaa tttcaaaacc ctgatactta tacatcacct cctggaaacg ggcaaaattt
    29521 tcggcagaga tttgcttaag caaaaccacg gctttacggg gggaatagcc tttttgtttt
    29581 gttgcctgaa taaatgcatc gcgaacctgt tcaaccgtaa gcccaagcat agtggcaaac
    29641 tcaagtgtat caaatggttt tacctgcctc tttaccatta aaatatcaaa aaccggctta
    29701 ttggatacca tgggcactcc atgccgatcg agaactagtc ctcgggcagg atactgtgtg
    29761 atgtagcgga gcacgttatt tgctgccgag aacttgtagg aactatcaat tacctgaata
    29821 taaaataact tgccaattat ggcagcaaaa acaagcatta tgattaccga cactacaaca
    29881 cgtctgctac tttcagctcg taacaccact accttgacat tttattggga gcccgtaaaa
    29941 gctcaatgat cactataaaa aacgttgaaa cagcagtact gagaataatt cgaaccagcg
    30001 ttaaccagaa gtgtttaaac gtaaatactt ccagaacgaa tagagtaaag tgatgaacta
    30061 gcacagcaat tagggcatat acaacaaacc actccagtga ggtgttcgta acatcgggga
    30121 cacccctgat atcctgaacc gatttaaaac tccatcggtt taaaattact ggcctaaaat
    30181 aggctagaac cagcgaagcg gtggtatgga gtccaagtgt gttggaaaaa taatccacag
    30241 caaatcctag tgcaaaacca ataataagca acatccagcg ggtaattcgg tagggaagca
    30301 ataaaataat aagtatgtag atatacgggt ttacataacc catgaaattg atgttattaa
    30361 gcaccagaaa ctgcagtaaa agcagtatta ccgttaaacc taaatatttt agaatatcgc
    30421 tgtacatcac ttcattgttt gcaaacgatc aacctcgctt ttcacctttg aatcgaccaa
    30481 gtacacatag tcgagggttt taaaatcggc gaagagttca accgtaattt gtaggaaact
    30541 accatcctta ttgtaaaaat caacaacacg acctattggg atatccctgg ggaagatggt
    30601 tgaaaagcca ctggttacaa tggtatcgcc aactgccaca ttaacatgaa ggggaatttc
    30661 atcaagtaca acacgcctgt aattcatgcc atcccacgaa accgctccaa aagcgcccga
    30721 cttcttatgc atagccgaaa cccttacatc ggtatttaac agtgaaatga ctgaggcaaa
    30781 atgtttagtg gtagccgata caatgccaac aacgccgttc ttacatataa cgcccatctc
    30841 cttgtaagca ccgtcttttg atcccgcatc gagggtgatg tagttatggc ggctgttaat
    30901 tgagttatca ataaccctgg ctggaatata ccggaataaa ccggaagtgt cctcaattct
    30961 catgaatgaa tcctgaagca tttgcagctt aagcctgtac ccctctattt cgtttcggag
    31021 cctgacgttt tccagtagga gtagtacgtt tgaacgctgc agttggaaaa ggcctgttac
    31081 ctgtgatgct tgcttatgcc agagtccgga aattgactta gttactgccc caacctgcga
    31141 tttctggtag tacgtagtgt taaagtatag cgaaagcgat atccctaata gaattaggaa
    31201 tatcagatga atgtaaaggc gctttagaat ctcgtacagg ttattcatcg caaaagcatc
    31261 gcttgagtta cctcatcagg aatgggaacg acagattttt caatgctata ctggtacccc
    31321 ttgcaaccgc atgtaaagga tcctcagcaa cgtggaaagg aatgttcact ttctcggtta
    31381 accgtttatc gaggccacgg agtaatgctc caccaccagc caagtatata cccttttgaa
    31441 caatatcggc ataaagttca ggtggggttt gttctagcac gttaaaaata gccgattcta
    31501 tttttgaaag cgatttatcc aaacagtaag ctatttcctg ataagaaaca ggtacctcaa
    31561 tgggtaatgc tgtcattagg tttggcccac gaacaataaa gtcagccgga gggttgtcca
    31621 ggtcaggaag agctgaaccc acatttatct tgatatcctc agcggtacgc tcaccaattt
    31681 ttacgttgtg ctgctgacgc atgtatgcct gaatgtcggc tgtaaagcca tcgccggcaa
    31741 tacgaaccga cttgttgcat acaatacctc ccaaggcaat aaccgcaatt tcggtggtac
    31801 caccacctat gtccaccacc atactaccct caggagccat aacatcgagc ccgataccaa
    31861 gggcggttgc catgggttcg taaatcaggt aaacatcacg accaccagca tgttcagctg
    31921 agtcgcgtac agcacgaatt tcaacttcag tactgccact cggaatacct ataaccatgc
    31981 ggagcgatgg agtaaaaatt gggtttttgg tattaatcat gctaatcatc ccacgaatca
    32041 tttgttcagc cgcattaaaa tcggcaatta ctccatccct gagcgggcga atggttctga
    32101 tgttctcgtg ggttttacca tgcatagccc gggcctgttc tcctatggct atcattttcc
    32161 cggttgagtg atcaattgcc actatggaag gctgatcgac aactatttta tcgttaaaaa
    32221 gaatgatagt gttagccgtt cctaaatcga ttgccagctc ctgagttaaa aacgaaaaaa
    32281 gtcccatatc gaattaatat ttatttttct aatgtttaaa gtgtcgtatt ccagtaaaaa
    32341 tcatggcaat gccgttggca ttgcagaaat tgatgctctc ctgatccttt attgacccgc
    32401 caggttgaat aacagccctc acccctgcct cgtaggcaag ctgaacgcta tccgggaatg
    32461 ggaaaaaggc atcggaggcc attacagccc cattcaggtc aaatccaaag ctctgggctt
    32521 ttgcaatagc ttgctttaca gcatcaaccc ttgatgtttg ccccacgcca ctccctacta
    32581 gctggaggtt cttaactaaa acaatggcgt ttgatttggt atgctttaca attttattag
    32641 caaaaagcaa atcgttaacc tcgtttggcg tggccgattt ttgggttacc agttgcagca
    32701 tcgaatcgga ttcggtaacc gaatcgcgct cctgcattaa aactccattt aaggcagttc
    32761 ggtaaacctg ttgcggcagg ttaaaatcct tcatcctcag aattatcctg ttcttcttgg
    32821 cttgtagcac ttctagagca gcattatcaa agttgggggc aatgagaact tcaaagaaga
    32881 ttttattaat ctccttagcc gtagtctcat caattgaccg gttcgatatg ataatgccac
    32941 caaaggccga tacaggatca ccagcaagtg catcggccca agcctgaagg atactggggc
    33001 gtgaagcaac gccacaggcg ttattatgtt ttaaaatggc aatggttggc tcttcaaact
    33061 ccctgataag gttaaccgcc gcatcgatat ccaataggtt attgtaggag atttctttac
    33121 cgcaatactg atcgaacatt tcatcgaaat tgccgtaaaa aacccctttc tgatggggat
    33181 tctcaccata cctcaatccc ctaacaccct ttaagctttt acggaagaag ctctgaggga
    33241 actcagggct aaaatagtcg taaatggccg tatcgtaatg cgaagatgtg gcaaacgcct
    33301 cggctgcgaa tatcctacgt tgctccagcg atgtggcaca atcctgctcc gtgagcattt
    33361 cgtataccct ttcgtattgc tccctgttcg atacaattaa aacatccctg aaattcttag
    33421 cagccgccct gataagtgag attccgccaa tatcaatctt ttcaattacc tcaggttccg
    33481 acgcgcctga ctcaacagtc ttttcaaaag ggtaaaggtc aacaataacc aaatcaatct
    33541 cgggaatgct atactcctgg agctgaaaaa gatcgttctc gttttcgcgc cgggcaagta
    33601 ttccaccaaa aacctttggg tgcaatgttt tcaccctgcc tccaagtatt gagggatagc
    33661 ccgtgagctc ctccacagca acaaccggaa tccccaaccc cttgatgaaa tccattgttc
    33721 ctccggttga gtaaatggta acattttttt cgcctaaata ctttacaatt ttatcgagat
    33781 tatccttgtg gtaaaccgat attagtgcag tctttatagt tttcaagcca tccatcttgc
    33841 tagtattcaa ttacttgtaa aaaatatgaa tcctgtaacc tgcccctaaa attggttaaa
    33901 tcctttgaaa aaccaatact tagcaattaa attttttcaa catcatttaa aataattgac
    33961 actataaaat gtgacatttt gccaagttta acttaatcgc attaaacctt ccgtgtgtca
    34021 gccattctaa aaaatgttac actatttaac aatctaaggc cattgaaaag taaacctttg
    34081 gctatatttg ttgaatgagc atttggtaat atctgaaaaa aatctcaagc ataatactta
    34141 tagagaatga attctattcg tccgtttata atactaataa aacttgctcg cgaaagcatg
    34201 cagtttgctt accaatcagt aaaagcaaat aagcttagaa cctnnnnnnn nnnnnnnnnn
    34261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    34321 nnnnnnnnnn nnnnnnnnnn nnncgcgaaa gcatgcagtt tgcttaccaa tcagtaaaag
    34381 caaataagct tagaaccttt ctgtcgctgt ttggcatcac tattggcatt tttgccatta
    34441 tatcggtatt taccatgatt gacagcttag agaagaatgt gcgtgacagc ctacaggcgc
    34501 tagggaaaaa ggttgtatac gttcaaaagt ggccttgggg aggcaatgag gaatacccat
    34561 ggtggaaata cctcaataga ccagaaccaa ccctaagtga ccttgaggag atacgcagga
    34621 aaagcttact aaccgatgct gcatgcttta atgtagcata caataagcag ctaaagtata
    34681 atggcaacaa tatccccgat gctaccattt acggcattag caccgatttt gataaaattc
    34741 gaactttcga cttaagcgaa ggccgattct ttaaccagtt tgagattaac tcaggaagac
    34801 catatgctat aattggcagc gatattgcca aagacctttt tcaaggtgca aatccattgg
    34861 ataaaaccat caaaattggc gggcaaaaag ttacagtgat aggagtattt gccaaggaag
    34921 ggaaatcggc cattggggaa aacagcctcg ataacatggt aattattccg cttaaccttg
    34981 ctcgaaccat ggtcgaccta aggaattccg gcccatggat aatggttaaa gcaaaagatg
    35041 atactagcgt tgaggattta attgatgagt tacgtggcat actccgcgcc caccaccggc
    35101 ttaaacccct tgatgaggat gactttgcgc ttaaccaaat tgacctgttg aaggatagcc
    35161 tcgactccat ttttgcatca atcaattttg ccggaggatt cattgcattt ttctccataa
    35221 ttgttggagg atttggaata gccaatatta tgtttgtttc ggttaaggag cgcacaagcc
    35281 aaattggtgt tcaaaaagca ctaggggcaa aacgatactt catactaatc cagttcctct
    35341 acgaggcggt tctattggct gtggcaggtg gggttattgg cttgttgctt atttttattg
    35401 gcacacttgc agtaacccat gggttggact ttaacgtgtc gcttaccctg tcaaacatca
    35461 tccggggctt actcatatcg gcggttattg gaatagtatc gggatttgtg cctgcatggg
    35521 tggcatcacg tctaagtcct gttgaggcaa taaatacaaa ggcttagcca agcaaacgtt
    35581 ccacctctaa ggttaactcc acaaagttat caactgaaag ctgctcggct cgcttagtac
    35641 cgtatttatc gctaatctca atgccgttaa atgctgaacg gatggagttc cttaaggttt
    35701 tacgccgttg gttaaaggca gcttttacaa cccgttcaaa aacgctgtag ttacagccta
    35761 agtcatttac actatttcgt gttaaacgga ttactgccga ctttaccttg ggcggtggtg
    35821 taaagcatcc ctcgcttact gtgaacaaat actcaatatt gtaaaaggct tgtagaaaca
    35881 cactgagtat cccgtaagtt ctggaaccag gcttttcggc tattcgctgg gcaacctcgc
    35941 gctgaaccat tgctactacc tcaggaacaa aatcgcgctg ctcaattact ctaaaaaaga
    36001 tttgtgagga gatattgtag ggtagatttc ctattacagc aaattttcct ttcagcaact
    36061 ccttaacatc aaattgcaaa aaatcggcct caaccacgtt taaatcaggc aggtttcttt
    36121 taaggtagct gaccgattca ctatcaacct caactgctac aagtctacta ccaaaccttt
    36181 ggtaaagatc gaatgtaagc acgcccattc ccgggccaat ctcaagcacg gcatcacaac
    36241 catcggctct caggctttcg gtaatgcgtt gggcaacacg tttgtcgttc agaaaatgtt
    36301 gacctaaatg ctttttgggg cgcacttcgg gcatgttact ggggggtatt tgttaaaacg
    36361 gaatcaattc taggccttag cacaggtttt ggcttagata ctctagtcaa ggaatcgagc
    36421 tttaagccta aagcatttac ggttaaatgt tttgcaatca ctttccccga tttatcgata
    36481 agcaaactgc ctggtaagcg agctattcca taaagctgaa gtttgttgat gctgtaggtg
    36541 tcgtttagtg aaacctgata ccaacccagg gtgtcggatt tagcaaggtt gttcaacatt
    36601 aatgaatcca tctttactcc aaaactcacc acttcaaaac ccaaaggttt gtaggtggag
    36661 taaagatttt ttaagttttt gctattgcct attgattctt tatcccagaa acaaagcaga
    36721 atccattttg cccagattcc cggaatggaa atatatttcc cgtcaaatgt ttggaactga
    36781 aggggtggta caaccatgcc cggccttaat gaattttcaa tttgcgaaat actatcgtta
    36841 atcctatggt atccaatggt tttcttgtaa tcgaaaacta cttgtttttt agggtaaagc
    36901 aaggttaagc agctatcaac cttcctatat agcttggaat agctggtgta gggtaatagt
    36961 tgctttgaat ctaatgttgc atttacaatg aaaacagatg taagttcaaa gggattagat
    37021 gccacaaaca tgtatgcatc gttctgtaag ccatttttca acgaatcctt taattgattg
    37081 taaaccttaa tgagggaatc ttttaccaag acttcagtac tatcaatggc atcaattttt
    37141 acctgaagat tcttaagtgt ctgcgaaacc ttattaatat cattctgaaa attgactact
    37201 tgaatcgttt cgggcgaacc atcggcttgc caactattac tataggtaag tgttattttt
    37261 tcacccggct ttgccgatac tatcaacggg taatccttct tatccgttgt gatggctatt
    37321 acttgaggtt ctttggtatt aaaaacgatt gacccttctc ctttagggtc gagaagcatc
    37381 gagtcggcaa agttgcttcc aaaatcaggc accctaaagt aaacccaacg ctccgtccaa
    37441 tgtggaactt taatggtaat ttcaacccta tcgtaatggt ttgagcatgc agtagcaata
    37501 aaaactaaaa ttgaagcatg taaatgaaag atgatttttt ttgaaaaaaa atttatgggc
    37561 atcgcaaacg aattatatct gaatagtcct aattgatcag gtttcaaaat taacaaaaac
    37621 ccgaactaaa attggactaa agtcatcttt ttttgctaac ttgttgcgag tttttcagag
    37681 taaaaagtgt ttaaataaaa aatttttgag ccatgaaata cctttcattt gacaaagagc
    37741 agctggttaa cctggagtac tcgctaaacc gggaaattct aagcacaaac cgtgcaggtg
    37801 gatatttcag cacaacaatt attctatgca acacacgcaa gtaccatggc ttgatgattt
    37861 tgccaatcga tgagtttgac ggtgagaatc acgttttgct ttcgtgcgtt gacgaaactg
    37921 taatacagca cgggcaaacc tttaacttgg gtattcatcg ttatcccggg atatatgagc
    37981 cccgaggaca caagtacatc attgatttta gctatgagcc aacgcctacc cttacctacc
    38041 aggttggcgg tgtaattttc aaaaaggagt tgctatttgt acacaacgag gagcagttca
    38101 tgattcggta caccttagta gatgcgcata gctccacaca cctgcgaatt aaaccttttc
    38161 ttgccttccg caatgtacat aatgtttcaa aggccaatat gtgggctaac acaaaatttc
    38221 aaccatgtgc caatggggtt aagtataagc tttacacagg gttccctacg ctacacctac
    38281 agctgaacaa gaaaaacgaa tttgtagctt gccctgattg gtactacaac attgagtaca
    38341 tggaggaact gaaacgtggg tacgaggggc acgaggacct ttttgttcca ggttacttcg
    38401 agattccaat aaaaaagggc gaaagcgtaa tcttttcagc cgccaccgag gagcaaacgc
    38461 cttcgacctt acttaaaaag tttaatgcgg aaatactccg acgcccagcc aaggatagct
    38521 acaaagcttg catggaaaac tctgcccgac agtttattgt tcataaaggg aaaagctggg
    38581 atataattgc tggttaccca tggtttggaa gttggggacg cgattcgttt atatcgctac
    38641 cgggactaac attaggcatt gacgatccta aaatgtgcaa aacactgctg gactcgctta
    38701 gcgcaaagct caagaatgga cttttcccca acatgggaaa tgccacatcg caggccttta
    38761 actcggttga tgcacccctt tggtatttct gggctattca gcaatactac ctatatacaa
    38821 acgaccacaa ggaggtatgg cacgattatg ccaagaagat gaaaacgatc ctcaaaggat
    38881 tcagggatgg attaccattt aacattaaga tgcacgacaa cgggcttatt tgggcaggag
    38941 agcggggtta tgcccttaca tggatggatg ccgtggttta tggcaaaccc gtcacacccc
    39001 gaattggcta tccggttgaa ataaatgcct tatggtacaa cgctataatg tttaccattg
    39061 accttgccac caagaacaag gattaccgtt ttgtaaagga atggctacct attgccgaaa
    39121 gggcaaaagc atcgttcaac aatcttttct ggcttgccga gaagggctac ttagccgatt
    39181 ttgttgatga ggatggcccc aataccgata ttaggccgaa tcaaatcttt gccatttcgc
    39241 tgccatacac accactcgat gaagataaaa tgccaagtgt gcttaagatt gtcgaaaagg
    39301 agctatatac acccaaggga ctacgaacac tctcgccaaa caatccgcat tataagggga
    39361 gatacgaggg cgaccaaccc acacgcgact cagcctacca tcagggtgca gcatggccct
    39421 ggctcttagg agcctatgtt gaggctcatt tccgccttaa ccccgaacac gccattaatg
    39481 tggctgaaaa tattcttgaa tcgttccagg aagatattag tattcacggt atttgcacca
    39541 tttccgaggt ttacgatggc gatccccctc aacgtccaaa cggctgcatt tcgcaggcat
    39601 ggagcgttgc tgaacttata agaatatatc aaatggttga aaaacacaaa tcaggaaagg
    39661 gtaaataata tgagagttct gatgttcggt tgggagtttc cccctcacat tactggaggt
    39721 ttaggaaccg cttgctatgg acttacccgg gcattggcta aacagggcac tgaaattatt
    39781 tttgttgtgc ccaaagctta tggcgatgaa gaccagcgta gggttcgcct tgttagcgcc
    39841 gaagatattg aggtgtcgca ctcatccaaa aaccttgaag agttctggaa acatattaaa
    39901 tacctggaga tcagctcaaa catagttccc tatgttgatc ctgaggaatt tagtaaaatt
    39961 gttgagtacg ctgagaacga gagcgttgag tacaatataa acacatttaa aagaaaattt
    40021 aagttcacag gaaagtacgg ccctaaccta atgaccgagg ttacccgcta cgctattact
    40081 gctgcatcgg tggcttggcg ggaaacgttt gatgtaattc atgctcatga ctggcttacc
    40141 taccccgctg gcattgcagc caaaatggta tcgggaaaac ccttggttgt tcacgttcac
    40201 gcaaccgaat tcgaccgcag tggcgagaat gtaaatcaag cggtttacga tattgagcgt
    40261 cgaggcatgg aagttgccga catggttgtt accgttagca acctaacccg taacatcgtg
    40321 ataaaccgtt atggtattcc cgaacataag gtagtaactg ttcacaacgc cgttcatttt
    40381 aagggtgata ttgagcaaga atttgagcga ggggttgatg agaaagtggt tacattccta
    40441 ggaagggtta cttttcagaa aggacccgac tactttgtgg aggcggccaa caaggtgctc
    40501 aaacgcaccc aaaatgttag gtttgttatg gctggaagcg gcgatatgct aaaccgaatg
    40561 attaggcgtg ttgcgcaact tggcattgcc ccaaggtttc actttacagg cttcctcaag
    40621 ggaaacgagg taaaccagat gttccagcta tcggacgttt atgttatgcc atcggtatcg
    40681 gaaccatttg ggataagccc gcttgaggcc atgcgctcaa gcgttccggt tattatatca
    40741 aaacaatcgg gtgttgccga ggtgcttaag tatgccatta aggtcgattt ttgggatatc
    40801 gatgcccttg ccgatagtat ttacgggct
//
LOCUS       DAOSAL010000017        37577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_820_length_37577_cov_49.720537, whole genome shotgun sequence.
ACCESSION   DAOSAL010000017 DAOSAL010000000
VERSION     DAOSAL010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 37577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37577
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_820_length_37577_cov_49.720537"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..99)
                     /locus_tag="PK049_04100"
     tRNA            complement(26..99)
                     /locus_tag="PK049_04100"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(63..65),aa:Ala,seq:ggc)
     gene            281..967
                     /locus_tag="PK049_04105"
     CDS             281..967
                     /locus_tag="PK049_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOK60774.1"
                     /translation="MELKKNPKADLQNKKLLFFEIGLALSLGIVLLAFEWTTTDTVNV
                     DLAQTTEVKVEQEIIPITNRDEVKPPEPPKQITVSDVIKVVDDNVNINDNTDIFDSEF
                     SEDKAVTVMEFKDEEQEEEEYTPFVIVEDMPSFQGGDLNNFRVWVQQNLKYPDVAAEN
                     GIQGRVIINFVVEPSGKVTNVKVVRGVDPSIDKEAIRVVSSSPIWKPGSQRGKPVRVQ
                     FTIPIIFVLQ"
     gene            1087..1767
                     /locus_tag="PK049_04110"
     CDS             1087..1767
                     /locus_tag="PK049_04110"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOK60775.1"
                     /translation="MEAKKSKKADLESKRILFFEVGLTVSILLAILAFEWKSPAGINT
                     SLIAELGNNNGTIELPPITTDPKEMVKPEPPKVVISEKIIVVDNNVEPNYNTDIFEEP
                     KDAYVPPVIRIEEKEVDETFERVEVMPTFDKGDANHFRNTYVIPNIRYPQEAIDNGVA
                     GVAFVEFVVERDGRVTNVKVLNKIDKSLEREAIRVVSSSPRWEPGFNNGVKVRVKFVI
                     PIKFVLNN"
     gene            1835..3052
                     /gene="hflX"
                     /locus_tag="PK049_04115"
     CDS             1835..3052
                     /gene="hflX"
                     /locus_tag="PK049_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOK60776.1"
                     /translation="MINPISTAKKQERAVLVGVLLPFQKEQEITDYLDELEFLTETAG
                     AITLKRFIQKIDEPNPRTFIGSGKVQEIAAYIDDNDVDMVIFDDELSPTQLRNLEHEF
                     KRKVLDRTNLILDVFAQRAKTAYAKTQVELAQYQFLLPRLKRMWTHLERQRGGIGLRG
                     PGEREIETDRRIIRDRIAKLKEELSKIDRQMATQRKNRGSVVRVALVGYTNVGKSTIM
                     NLISKSDVFAENKLFATLDTTVRKVVIGNLPFLLSDTVGFIRKLPHQLVESFKSTLDE
                     VREADLLLHVVDIAHPNFEEQINIVNQTLHEIGGGDKPTILVFNKIDAYTWTSKPDDD
                     LTPSTRENISLEELKRTWISKKGTHTIFISAKQKINIDEFRALLYKMVREIHVTRYPY
                     DNFLYPDITEANL"
     gene            complement(3033..4025)
                     /locus_tag="PK049_04120"
     CDS             complement(3033..4025)
                     /locus_tag="PK049_04120"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOK60777.1"
                     /translation="MRVAVVGATGLVGSVMLRVLEERNFPVDELIVAASEKSVGKTVK
                     FKDAEVKVCSVADAVKQKPAVAIFSAGSAASKEWAPKFAEVGTTVIDNSSCWRMYPEI
                     PLVVPEVNAGVLTPNHKIIANPNCSTIQLVVAINPLHLKYRIKRIVVSTYQSVTGTGV
                     KAVNQLFAERQGHEADMAYPHPIDLNCFPHGGTFLDNGYTTEEQKLIDETRKIIGDQS
                     IMVSPTVVRIPVIGGHSESVNLEFESEFDINEVNEILRSAPGVVVQDNPATNHYPMPR
                     FAEGKDDVFVGRIRRDVSQPRSLNLWVVSDNLRKGAATNAIQIAEYLNAKGWLR"
     gene            4098..9725
                     /locus_tag="PK049_04125"
     CDS             4098..9725
                     /locus_tag="PK049_04125"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOK60778.1"
                     /translation="MRVTVITFLTLLASLTFGQQVVTFNFESGDLSEWSQKPENRWSI
                     STNSPLSGSASLRHSFDNSIAYTDTIYRALPVWDIEKGDVTWRFLIRHGYDPSASNSW
                     WVYLMANDEIANSNESGYAVGVNLTGSDDLLKLWRIDNGSAQVILTSTLNWQTQIGTG
                     KAGAIEVTRTFDGNFTLRASVSGNFSTLTTYGTVYDFSYKNFNIFGIGYRYSSAQDMK
                     LWADDISFIYQPINPNDRTTAVLNPSQQVVPTNIISTSTLEENCVDVLRFRITDAGSG
                     DGLPTYVKRMTIKNPDPENASWSSLLGGITLKKDGQLIPTQTVSITDQQVTIEVDSSL
                     ATVPDGQYTEFTFGVYLKDSNIPDNLNLLFEIDNENHGFQSSLWGSGFAQTFPAKVQS
                     GIFTIRVNATQAMFRNISPIAIIGKPFGLEVAATDTNSNTDADYGGQITLSLSEGNGS
                     LSSVNGLSRVAIQGIAKWDSLLYDRYGSIRIMAAGENINTDISDEIVIGYDTTSYAAV
                     PGNQVSSFDISSMATSPSQANELMRFRITDAGSDGAPTHLMKIRLNRANTVNAATLNK
                     AIEGVMVKLGDNYISFSSVDIKTSTIDITFPPGALTIPDGQTIELGIWVYLKNSGISD
                     NQTIQLYIDKLNPGFEAYDIGSRFATKFPTNINSAIATIRVSATKLNFTSTPTRVGVN
                     EPFTVKVKPTDANGNADVNFAGNVELLLGSGSGELELISENPAPIINGETQLEANYST
                     PGIFTLIARNPSLNDAVSGNITSGDADGFAAPLIQPTDTVIFTQNNSYAAAAQEIIRF
                     KLKDPGSTDGLPLNVSRIKLLAFNPNSLPELARMVQGFVLDNGTITIAPKSYTFSGNV
                     LQIDFNEGDVVVNDTDSANFILKVFLKDRNLVDAFRFQFYIPSSNHGWTAFDNSTAFN
                     STFPSTIYGRPCRIEVQADRLKFLTQPFGVNPNEPFSVSVMACDSRGNVDREFQAYAA
                     LEVYNGTGNLIVNPLLQPLDNGIATWNGVKLTATGTYRLKSYFGWLADAISENIYCGY
                     NHTCPVSEYFESILPAWSGIENWTTSRTDPITGEYSLAHAGDPAANQSVLSIPIETTL
                     LGKASEWNITIRNGNWDPSSENFFYLAFASTHENPMDTEAEGYAVGINPSSGNDNIAV
                     WQFKEGKRTNIIQSAFDWNENDQVKITVTLSPNGTIKLWFTPKSTGLKIFGGEAKIAV
                     PQCSYMAFVFAYTASRAGQLWIDDMSLCTTDFAPVISSARASNLNTVRVNFSKPVSIQ
                     SAQNVQNYKLKTLTNQNIGIQSIRIDGNQMVSITTQKLPFEKLILVVRNIEDMNGYSL
                     PDSIELGFTAQGNMGRLIFNEIMANPVPSNGLPEYEYIELFNPGTDTVFTEGWKLTLN
                     EKTVTMPADTIPPKSYALVGGTTAMSNFNVYGKTIAITSFPALLNDGMLLRLYDQQEN
                     LIGFANYTKEWYNDSIRNVGGYSLECIDYSNLAEGKNNWRASIASEGGTPCAPNSVMA
                     INPDVTPPQLCYIEVPDAKTIRLCFSEAMDSLTATIHENYITELGINNINIGELYTRV
                     TIELSEPLEAEKIYEISISGLTDFAGNAMPDTAIRVGLPQLASEGDIVINEILFNPYV
                     GGTDFVELFNRSSKCFDLSQIKIANRNETDLSLKDPDSASPLPWLFFPGDYAVITTNP
                     NQVTQFYSCQNPSSFIKTSRLAAFNNDKGYVVLLNSENEVIDELRYTEKMHNKLLNDF
                     KGVSLERINPSMPTNQSSTWQSAAQAAGFATPTYRNSQYSELSGEKSNFSLTPETFSP
                     DGDGRDDYLLISYQLPEPGYVANIRVFNANGIEIYRLANNMTLGTSGQLRWDGTDTKN
                     RRVNSGIYIVYFEYFNLSGEVKREKKTCVVGYR"
     gene            9784..10572
                     /locus_tag="PK049_04130"
     CDS             9784..10572
                     /locus_tag="PK049_04130"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOK60779.1"
                     /translation="MEGYIELPKCTLRYRISGSGPAVVLLHGYLESLTIWEELTMELE
                     PDYTVLAVDLPGHGKSICKEEVIGMDFMADSVSAVMRAVSISKAHLVGHSMGGYASLA
                     FAERYPNWLTGLCLLHSTPNADTHEKRSARMADIKLVKEGKKEQIVENNIPRLFANHN
                     QQKLMGEINHIKSIARKTSESGIVGALNGMAQRPDRNHIIDQALFPVTMIFGKHDNLI
                     SMEVAQTLEERHRKARTIYLENSGHMGFIEEPEQTFSAIKSILK"
     gene            10789..11247
                     /locus_tag="PK049_04135"
     CDS             10789..11247
                     /locus_tag="PK049_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HOK60780.1"
                     /translation="MEKSKYPYGTKQPQFDRRYLQMAKIWAKNSYCKRRQVGALIVKE
                     RMIISDGYNGTPSGFENICEDETGKTKPYVLHAEANAITKVSKSSNSSEGATLYVTSS
                     PCMECSKLIIQAGIRRVVYCDEYHNLDGIELLKRAGIEVVKIEIEDINEF"
     gene            11263..12894
                     /locus_tag="PK049_04140"
     CDS             11263..12894
                     /locus_tag="PK049_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOK60781.1"
                     /translation="MSYPNSRKDIIYPVVIAIVLVIGVALGLILGGRVEKPNLLVYPR
                     TDKLSNVIRYIADEYVDTISTETLVENTIPAVLKNLDPHSVYIPAKDLQSVNEPLEGG
                     FDGIGIQFNMNNDTVVVINTIPGGPSEKLGILAGDRIVKVNGKNIAGVKFPMDSVPKL
                     LKGPTGTRVKVSIKRTDVPDLIDFDIVRDKIPIYSVDVAYMPTKDIGYIKINTFAKTT
                     YKEFLEAMVKLTEKGMTKLILDLRSNSGGYMDAAINIANEFLPANRLIVYTQGKNRPR
                     QEAKSNGKGFFQDLPLTILIDEFSASASEILAGAIQDNDRGIIVGRRSFGKGLVQEQV
                     YFSDGSALRLTIARYYTPSGRSIQKPYKPGDEEYFYEIGNRYLHGEFQNADSIQFADS
                     LKYYTIGGRVVYGGGGIMPDFFVPLDTVGITPYYQQVSRRNLIYRYAFKFADEHRKQT
                     RALKSFDEVDRYLKKFNILTDFVGFAKQNGVEPDPNDLAQSKLIIETQLKATIARNII
                     DNEGFYPYIRYIDETLIKAIDLCEKGNCILPLNRN"
     gene            13294..13848
                     /locus_tag="PK049_04145"
     CDS             13294..13848
                     /locus_tag="PK049_04145"
                     /inference="COORDINATES: protein motif:HMM:NF023096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HOK60782.1"
                     /translation="MEIDTNQTEEIIEVGKANRLALLMLFPIAAITIIPFVIIYGFGP
                     LANGVIFLKNHQLQILVAFVLLIFVHEGLHGVTWAFFAKNGFKSIHFGIKWSYLTPYC
                     HCNEPMKRKHYMLGGIMPGLITGVIPIIIAMVTANGWLLISGIFLTAAAGGDILVLIR
                     VLKYPSNLIFMDHPKEIGFIVFKS"
     assembly_gap    13852..13951
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            14168..15364
                     /gene="kbl"
                     /locus_tag="PK049_04150"
     CDS             14168..15364
                     /gene="kbl"
                     /locus_tag="PK049_04150"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HOK60783.1"
                     /translation="MYGKIKNDLQQQLENIKAEGLYKTERIITTPQGVEIVANGKKVL
                     NFCANNYLGLSSHPAVIEAGKKAIDEWGFGMSSVRFICGTQDLHKQLERKLSEFLGTE
                     DTILYSSAFDANGGIFEPILGEEDAIISDELNHASIIDGVRLCKAQRFRYKNNDMADL
                     EAKLQEAANCRYKMIVTDGVFSMDGIIAQVDKICDLADKYDAMVMVDDSHATGFVGKT
                     GRGTAEYCNALGRVDVISTTFGKALGGASGGCISGRKEIIDMLRQRSRPYLFSNSLMP
                     SIAGATLKVLNMLTETTELRDRLWENTQLFRKGMTEAGFRIVEGVHPITPIMFGHLPN
                     DAKLSQDFAAALLEEGIYVIGFYYPVVPKGKARIRVQISAAHTREHIQFAIEKFTKVG
                     KKLGVI"
     gene            complement(15461..16075)
                     /locus_tag="PK049_04155"
     CDS             complement(15461..16075)
                     /locus_tag="PK049_04155"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014708513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HOK60784.1"
                     /translation="MHIAIAGNIGSGKTTLTGLLAKHYKWKPQYEDVDENPYLNDFYE
                     DMQRWSFNLQIYFLNSRFSQIVEIHRSGHDVIQDRTIYEDAHIFAPNLHDMGLMSTRD
                     FNNYLSLFNLMTSLVKPPDLLIYLRATVPTLVRQIQKRGRDYEKNIRLDYLQRLNERY
                     EAWIEGYKLGKLLIIDVDNLNFPDRPEDLRFIINKIDAQIHGLF"
     gene            complement(16216..17742)
                     /locus_tag="PK049_04160"
     CDS             complement(16216..17742)
                     /locus_tag="PK049_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HOK60785.1"
                     /translation="MPLLNSILNWVNTKRLYSIDLFRKYPFEVQRDVLFELLKQGSKT
                     EYGARYGFDGIRTIEEFQKRVPLVDYETIKPYIERLRQGEEKLLWPTDIKWFAKSSGT
                     TQSKSKFIPVSNEALEDCHFRGARDVLAIYMNLYPENNLLSGKGLTLGGSHQVDNFNM
                     NSFYGDLSAILIENQPWWAEFIRTPSQKVALIPDWEEKLEKLTHETLNENVTSLAGVP
                     SWNLVMIKHLLNYTGKSNLLEIWPNLELFMHGGVSFAPYRDQFQKVIPSPNMHYMETY
                     NASEGFFAIQDDPASDSMLLMLDYGIFYEFVPLDELTKPNPTALTIADVEVGRNYAMV
                     ITTNSGLWRYLIGDTVTFTSKHPHKIRITGRTRHFINVFGEEVIVENAERALEKACKA
                     TGALVAEYTAAPVYMGTDRKGCHEWFIEFVKQPSSIEEFARVLDSTLCEVNSDYEAKR
                     AKNVTLDFPVVTQAVQGTFFEWMRQRGKLGGQNKVPRLSNTREYIDELLAIHNQLKSK
                     "
     gene            complement(17797..18741)
                     /locus_tag="PK049_04165"
     CDS             complement(17797..18741)
                     /locus_tag="PK049_04165"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HOK60786.1"
                     /translation="MTKLLTTILLSILTITVNAQVTDAENQLKEIKTDSLSGWKRGLA
                     VGINANQTSLTNWAAGGQSSFALNTVFTGFVSYKSPKTAWDNTIDLGYGFLSQEGVKY
                     TKKTDDKIDLMSKFGREAFKNFYYAVLLNIKTQFTTGYKYPTDSTRLKISNFFAPAYL
                     VGALGMDYKPNTHLSVFAAPLTGKLTFVTDTMLSNAGAFGVKKGETLREEIGGYVRVV
                     YTKNDFNAEWLKNLTFTTKLDLFSNYLDNPQNIVVNWETLVIMKVNKYINVNLATQLI
                     YDDKVKISKDNNGDGVIDSSGPRVQFKEIFGVGITFKL"
     gene            18842..20641
                     /gene="typA"
                     /locus_tag="PK049_04170"
     CDS             18842..20641
                     /gene="typA"
                     /locus_tag="PK049_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HOK60787.1"
                     /translation="MKGIRNIAIIAHVDHGKTTLVDKMIIQGKLFKDHENPGELILDS
                     NDLERERGITILAKNVSVNYKGYKINIIDTPGHSDFGGEVERVLNMADGVLLLVDAFE
                     GTMPQTRFVLQKALAIGLKPILVINKVDKPNCRPEEVQEQVYELMFSLDASEEQLEFP
                     TIYGSAKQGWMSNDWRKPSNDITPLLDCIIDYVPAPKQLKGTPQLLITSLDYSNYVGR
                     IAIGKLHRGTLRAGQNVSLAKRNGQVMRTKIKELLVFEGLERKKVDEVSSGDICALVG
                     IEGFDIGDTIADYENPEPLPTIAVDEPTMSMLFTINNSPFFGRDGKYVTSRHLKDRLM
                     QELEKNLALRVEETDSADAFTVYGRGVLHLSVLIETMRREGYELQVGQPKVIIKEIDG
                     VKYEPIEYLTIDVPEDVSGKAIEQVTRRKGTLISMDRRHDRVHLEFEIPSRGIIGLRN
                     IILTATAGEAIMAHRFKAFEPYRGEIETRTNGSLIAMESGTAYAYALGKLQDRGRFFI
                     DEHEEVYTGQVVGENNRSGDIVVNVTKSKKLTNMRASGSDEKIFLAPPIRFSLEEALE
                     YIQEDEYVEITPKAIRLRKIYLDENERKRRAKA"
     gene            20729..21259
                     /locus_tag="PK049_04175"
     CDS             20729..21259
                     /locus_tag="PK049_04175"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOK60788.1"
                     /translation="MASILGAYSISYKGLKLGKHSFDFEINNDFFAEFPEGEVKEGTL
                     TAKVMMNKQNNLLEFDIVIKGKVKVTCDRCLEQFFLPIEYKGFLVAKIGNEEVEDQAD
                     IIFLTEDDHEVNLAQYLYESIHLSLPLKRYHGLKGSSIEDCDQEMLKYIKFEDDDQVE
                     VDPRWQALKDLLNNNN"
     gene            21285..21470
                     /gene="rpmF"
                     /locus_tag="PK049_04180"
     CDS             21285..21470
                     /gene="rpmF"
                     /locus_tag="PK049_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOK60789.1"
                     /translation="MAHPKSRISKQRRNKRRTHYKAEAPTLSTCSNCGAAVEYHRVCP
                     ECGYYRGKLAIEKAVNA"
     gene            21498..22442
                     /locus_tag="PK049_04185"
     CDS             21498..22442
                     /locus_tag="PK049_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate--acyl-ACP acyltransferase"
                     /protein_id="HOK60790.1"
                     /translation="MIRIGVDAMGGDFAPDAAVSGAVLALKHLNADERIVLFGDQQRI
                     LEILARENANPTDFDIVHTSEVIEMGDHPSKAFAKKTDSSIVKGFMALAAGKIDGFAS
                     AGSTGAMMVGAFMVIKQIPGVIRPGIAGFIPVSESKLNVILDVGLNPDCRPDVLYQYG
                     KLGSIYAQQVLGIDNPRVALLNIGEEEEKGNLVTKSAFEMMKDSTDYTFVGNVEGHHL
                     FHDKKADVVVCDGFVGNVVLKEAEAFYHLLKKFSPENKFIERFNSENYGGTPVLGVNK
                     PVIIAHGASSPVAIMNMIKQTREVIVSDLCEKIKQAFM"
     gene            22445..23455
                     /locus_tag="PK049_04190"
     CDS             22445..23455
                     /locus_tag="PK049_04190"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019131263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOK60791.1"
                     /translation="MGKITAAITGVGGYVPEYILTNEELSRMVDTTDEWIMTRIGIKE
                     RRILKGEGLGTSDMAVHAVNELLRKTNTHPDEVDMLICGVVTPDMQFPATANIISDKC
                     GIKNAFGYDMNAGCSSFLYALVTASKFVESGSHKKVVVVGADKMSSITDYTDRTTCPI
                     FGDGAAAVLLEPNHDGFGVIDAKMQVDGSGRKHLHQVAGGSCYPASFETVAKRQHFIY
                     QEGQPVFKAAVSNMADVSVEMMERNNIKPEDLAWLVPHQANMRIIEATANRMGIDKDK
                     VMINIQRYGNTTSATIPLCLWEWESKLRKGDNIIIATFGAGFTWGAAYVKWAYDGDKI
                     AK"
     gene            complement(23524..24051)
                     /locus_tag="PK049_04195"
     CDS             complement(23524..24051)
                     /locus_tag="PK049_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOK60792.1"
                     /translation="MKLLMAIFWVLLSLQVGSEINFEPKQISREVKRISGSQTPIAKE
                     IMAYKTGKFFTYTNANPVSFSYVGRVFTCRTEGCKASDDNVNANGSEYFDYFILFDRS
                     GKILSLQIYSFEATHGQEITLKSWLNQFVGYNGKNTLTVGKEIDAISGATTSVHRLTE
                     DVIDKTRKLQEILHQ"
     gene            complement(24061..24708)
                     /locus_tag="PK049_04200"
     CDS             complement(24061..24708)
                     /locus_tag="PK049_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60793.1"
                     /translation="MFSGSKGLREGRQKASKAYMNQPAFTGKLEFYGVKNLNVGLSGY
                     FGKSNSKLYSNLSKDSLAKIQRADSSVVGISMVGLDTRYNFKGVKLTGQLYYISLSNT
                     NEYNVFTRTGTKLNDLGSAMLGYYVEVGYDIFQTLADTKKSLMPFIRYEAYNTHHSVE
                     APVVTNKSYSNTIITTGLTYALEKGVVLKADLQFYKSKAENSFSKTFNAGIGVNF"
     gene            complement(24811..25266)
                     /locus_tag="PK049_04205"
     CDS             complement(24811..25266)
                     /locus_tag="PK049_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60794.1"
                     /translation="MKRVLYVFGLLVISFGLKAQTDTIPNQQQNTAQTLMNSKPGLQI
                     GGYGEVHYNQPLKSNTHTLGTLDAHRMVLFFGYNFSSKTQFVTEVEIEYAREVWVEQM
                     FIQYRLNRYANFRAGVLLVPMGIINEYHEPTAFNGVERPIIDNKIAPTT"
     gene            complement(25911..26093)
                     /locus_tag="PK049_04210"
     CDS             complement(25911..26093)
                     /locus_tag="PK049_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60795.1"
                     /translation="MGAPWVELQRDTIRHAFGVTHVVSLTKPTATFHPRPISGSGFYA
                     SACFFRSIPSQGKGLG"
     gene            complement(26595..28952)
                     /locus_tag="PK049_04215"
     CDS             complement(26595..28952)
                     /locus_tag="PK049_04215"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOK60796.1"
                     /translation="MRIGKLNISADKIKMRIGAKLLLWVLTTSAAIMIVIGSFISIRV
                     NSIARDNAIKIAQAEAQKSAFRLKSELDLDMGFSRALAHALYIYPKFDTVTLDSIFFN
                     ILRNQVSNNPRYLTVWYSIEYSAFRPGYTKDYGRRSVTAYLSNGQVGIDIEHKNITGD
                     IVTSNYYASKTSNCEMLLDPYTFEYGGKEVLATSLSVPVRVKGKFAGLGGVDISLEKF
                     QTDIEKIKPYPGTNVSLIAGDGRIIADSNPENAKEYFANIYPELESKFALTQKVKDKQ
                     NHFFFSYNSGMEYLNILSILQPGESPNSWGLILSIPMSEIVKDARRSVFSTILVFILG
                     IILQAFAIWYISARISKPIKRTTELLNSIAQGDIDVNKKIYLHTGDELEEMSRSANKL
                     IDGLNYTEQFAREIGEGKLDTEFKLLSEKDKLGKALTEMQRSLIKAREQEEKRKEEEQ
                     QQIWATQGMALFGEVLRQHNDNITELSYLIIKNLVSYTGSIQGGMFIVNENDPNNTVL
                     EMTACYAYNRRKLMEKTVLPNEGLVGRCYVEKKTILMVDVPRDYIKITSGLGEENPRC
                     VLIVPLIANDIVQGVVELATFTPYQKYQIDFIEKLAASIAATLASVKINMRTAQLLTQ
                     TQQQAEEMRAQEEEMRQNMEELHATQEEMERKRHEQETIQAQLREDKTILESLLLNSS
                     DLIFQKDADGRYRRISNSLVSLLGLSSVEEAIGLNEFDLLPEDTARKLWDLTEDVARK
                     KSPVVDRKISLTFANGQEMKGSVSLYPLTSDAGSIIGVLGIFRQK"
     gene            29138..31429
                     /locus_tag="PK049_04220"
     CDS             29138..31429
                     /locus_tag="PK049_04220"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOK60797.1"
                     /translation="MRILNIKKCITFLVFLAVSIFAGLHAQEIDSLLSLSEHYRTVNP
                     DSAVSVARQALAKVDAVNQPDIAARVYFSLGYAYYNRNDFELALEHFNKSLMLAEQSD
                     NVQLKALLHNRIGNVYQLKSYYSQAIDEYKKALDYNYKIGSKVEIARTLVNIGTVFAI
                     TGNFSIALQYMLDALEIFEQSGETEGMAWTSLSISRLFSRLEINERALQYAENALNHY
                     RRLNNQTGITLAQTELANIYYHTGMHDKALDLLGKVIDENSRTRNIHAQAANYLLMGI
                     IYFDKKQYQQSEYYLNLSLLLKQQVNDSIDMAKLNRYMGELELQRGNFAKGITLLNKA
                     LNDAQKQKITTDLRDIYFSLSDAERRKGNYKKAYEYFTSYSALKDSVNASQIAKLEMQ
                     YDFEKREKEQELISRQRDAMQQARLERQRMATLLISIALVLSLALAAVVFRFYREKKR
                     TNQILIQQNAEILRQKNEIENQKQEIEAQRDLAERQRDQIAEQQKQITDSIKYASRIQ
                     AAVLPRSQQISTLLRDHFVLYQPKNIVSGDFFWVTQLTDGRIAIAAADCTGHGVPGAF
                     MSMLGITLLKELTATQHFENAADILLRLRRLVIASLNPAETDNDSHDGMDMGMLIVDR
                     KRMEAHYAGAYFPLLLIRDSSVQAPPTHSNPVIADGSTLYEIKGDKMPIGHHIIEERM
                     FTNHTFAIFENDLFYLLSDGYSDQFGGPKNSKFMMVNLKKLLLDIHHLPLNTQQSILQ
                     EKFDEFKGNEKQVDDILIMGFRV"
     gene            31939..34353
                     /locus_tag="PK049_04225"
     CDS             31939..34353
                     /locus_tag="PK049_04225"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOK60798.1"
                     /translation="MLLQYPFSVFSQPKSDSIAVRALIDSAANAKSPQVRIAFAREAY
                     HKAKATNSKHLLADATHSMGNALFRLNSYDAAIDSLQRALVLYNIVGDSAGTAKTYYT
                     LGSCYVRKSNYVKAIQLIEESVKLATSINDSALLANCYNSLGIVYYTLENFTASIDFF
                     EKAANYYEKAHSYKNYVRAKGNVGLSLTKIGNLEGARQIFFSTLNSYGKYMDSLMLAS
                     FYDNIGFFYESLGKVDSALYYHNQSLTISKTLSSTRGIALSNLAIGRCYTGIAQFGLA
                     IKYLLEAKRLAKLNGDEDLSAQANRQLSTCYEATGDFKNSQKCLKEFLEYTDKTFTKK
                     LITQVAQLHSMLQIEKQERENQQLQMQLELKSLNEQKDKKIITLYTIFSILLLLTLSV
                     IVYFAIQLSQKKKLLEHTNSQLFKFNNELDSLVKHRTRELNNAIDKIRELERIKSAFM
                     ANISHEIRTPLNGILGLSYYLSAPECTAEERSQLGEQVKRLGNKLVRIVDDILELSKI
                     ETNQVNLLLSEVNLNQLLDDVYKEFDAIAEFDDKNLFFRVNKSLPDDRAIVICDYYRL
                     RSIIIHLSENAFKFTHQGGVEMGYSINENSLLYLYVKDTGVGIPNDIQKKVFERFYKH
                     LDDSSPIFYDGLGIGLTIAMGYAIALGGSIKLQSSPGQGSTFTLVLPITVPNSNANKE
                     AFNFTGKRILVVEDDLINYQYLHALLSKTGATVIHVKNGEDAIEAATIERAADLILLD
                     IQLPFKSGIEATIEIRKINKQIPIIAQTATDSEYVSACRDAGCNAFVTKPIDPDELLN
                     LIKRFL"
     gene            complement(34403..35494)
                     /gene="ychF"
                     /locus_tag="PK049_04230"
     CDS             complement(34403..35494)
                     /gene="ychF"
                     /locus_tag="PK049_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOK60799.1"
                     /translation="MGLKCGIIGLANVGKTTIFNCMSSTKALTSNVATGTGKSNISTI
                     NVPDNRLYELEKFQPTERIVHTTVEIVDIPGLSKTTGKGEGNKFLADVRNCDALIHVL
                     RCFDDPSLPHIDGSIDPVRDIETVNFELQVRDLESVEKKLQKVEKAAKVGDKEAKKAF
                     EVLSVYKNHLESFKNASTVPVDEHDKKYVDDLFLLTTKPVIYVCNVDEKSAVNGNQYS
                     NRVKEFLKDEKTEILIIAGKVEAEISELESEDDRKAFLADVGLTEPGVNKLIRAAYSL
                     LNLETFFTVGPKEIRAWTIKKGMTAPQAAGVIHSDLERGFIRAEVMKYNDFITLGSEH
                     ACREKGKLFVEGKNYVVEDGDILHIRFNV"
     gene            complement(35574..36605)
                     /locus_tag="PK049_04235"
     CDS             complement(35574..36605)
                     /locus_tag="PK049_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOK60800.1"
                     /translation="MKQCPKYFKALILGLAILTACVPAKEFQALQDKNAKCEEEREKL
                     TVENEALTTKNSELDSKTRILQAKVDALTSDSINRAVTFNRINEELTTLKERYAELQS
                     LQDGVISGSKDETKKLLLQIQKTQEELQEKEDALKELERRLYQRKLGLDKVQANLDSM
                     KRELDARNARLVELERMLNAKDSVMRALRKKVADALFGFEGKGLTVSIQNGKVYVSLE
                     EKLMFKSGSYEIDSKGAAAIKQLIPVLEQNPDINIMVEGHTDDVPYRGTGGLQDNWDL
                     SVKRATTIVRLLISGSRIDPVRVTAAGRSQYLPIDRAKTPEARQKNRRTEIILTPKMD
                     EILQLLESN"
     gene            36770..>37577
                     /locus_tag="PK049_04240"
     CDS             36770..>37577
                     /locus_tag="PK049_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HOK60801.1"
                     /translation="MENKYALLSNIDSPEDIKRLKPEQLVDLSAELRQYIIDIVSENP
                     GHFASSLGVVELTIALHYVYNTPFDRIIWDVGHQAYGHKILTGRRDVFHTNRKYKGIS
                     GFPKRSESPYDAFGVGHSSTSISAALGMSVASRLKLENRKVVAVIGDGALTGGMAFEA
                     LNHGGGINPNMLVILNDNNISIDPSVGALKEYLLDITTSKTYNKIKDEVWDILGRIDR
                     VGPKTRSLIQKLENAIKAAILKQSNLFESMGFRYFGPIDGHDVVYLTRVLQ"
BASE COUNT        10828 a         8185 c         8120 g        10344 t
ORIGIN      
        1 cttacttgcg tatccctttc aaccgtggag gtaaggggag tcgaacccct gacctcttgc
       61 atgccatgca agcgctctag ccaactgagc tataccccca tgaaataacg ctgcaaaaat
      121 aaaaactttt taattatact gccaatttca cgcaattttt tttaacatga tttaaaaaac
      181 tacgtataat cattggaaga taaataaaat ttttattgct ttgtagctca aaataaatta
      241 ggaaattaac cgttatacca agagccaaat acgcaaaaat atggaactaa agaaaaatcc
      301 caaggctgac ctgcaaaaca agaaactcct attctttgag ataggacttg ctttatcgct
      361 gggtattgtt ttgctggcct ttgaatggac aaccaccgac accgtaaacg ttgaccttgc
      421 ccaaaccaca gaagtaaagg ttgagcagga aattattcct attaccaacc gcgatgaggt
      481 taaaccccct gaaccaccca agcagattac cgtttctgac gttatcaagg tagttgatga
      541 taacgttaat atcaacgaca ataccgacat ctttgatagc gaattctccg aggacaaggc
      601 tgttacagtt atggaattca aggatgaaga acaggaagag gaagagtaca ctccattcgt
      661 tattgtagag gatatgccct cgttccaggg tggcgatctc aataacttcc gcgtttgggt
      721 acagcaaaac ctcaagtatc ccgatgtagc tgctgaaaac ggtattcagg gtcgcgtaat
      781 tatcaacttt gtagttgaac ctagcggaaa ggttaccaac gttaaggttg tacgtggcgt
      841 tgacccctct atcgataaag aggctatacg cgtggtttct tcgtcaccta tttggaaacc
      901 tggttcacaa cgcggtaaac ccgttcgcgt tcagtttacc attccaatta tctttgtgct
      961 tcagtagttt attaaataag attagaatac ttaaccccgc tttttgcggg gttatttttt
     1021 tattatacac aatgaaaggg gtatgattat cgttttatta ctagaaaccc tttaacataa
     1081 aaagttatgg aagcaaaaaa atcgaaaaaa gccgacctgg aaagcaagcg tatcctcttc
     1141 tttgaggtag gattaacagt atcgatccta cttgctatcc tggcctttga atggaaaagc
     1201 cctgctggta tcaatactag cctgattgct gagcttggta acaataatgg cactattgag
     1261 ttaccgccta tcaccactga ccctaaagag atggtaaaac cagaaccgcc caaagtggta
     1321 atctccgaaa agattattgt ggttgataac aatgtagagc caaactataa taccgatatt
     1381 tttgaggaac ccaaggatgc ttatgttcct ccagttattc ggattgagga aaaagaagtt
     1441 gatgaaacct ttgaacgtgt tgaggtgatg ccaacctttg acaagggcga tgccaaccat
     1501 ttccgtaaca catatgttat tccaaatatc cgttacccac aagaagcaat tgacaatggg
     1561 gtagcaggag ttgcttttgt tgaatttgtt gttgagcgcg atggcagagt aacaaacgtt
     1621 aaggtattaa acaaaataga taaaagtctt gagcgggagg ccataagggt tgtttcgagt
     1681 tcgcctcgct gggagccagg attcaataat ggtgtaaagg ttagggttaa attcgtaatt
     1741 cctattaaat ttgtgctcaa taattaaaca tttactttga taaaatcgtt aaagaaaacc
     1801 ctgattattc aggttttttt attgatacag gcaaatgata aacccaattt cgacagcaaa
     1861 aaaacaggaa agagcagtac tggttggtgt tttgctccct tttcagaaag aacaggagat
     1921 aaccgattac ctcgatgagc tggaattcct aacggaaaca gcaggggcca taaccctaaa
     1981 aaggtttatt cagaagattg atgaacccaa cccacgcaca ttcattggta gcggtaaggt
     2041 tcaggagatt gctgcttata ttgatgataa cgatgtggat atggtaattt tcgacgatga
     2101 gctctctcct acccaactcc gtaaccttga gcacgaattt aaacgcaaag tgctggatcg
     2161 aaccaaccta atactcgatg tgtttgccca acgggcaaaa acggcttacg ccaaaaccca
     2221 ggttgagctg gcacagtacc aattcttgct tccccgccta aaacgaatgt ggactcacct
     2281 tgagcgtcag cgcggaggta ttgggctacg aggccctggc gaacgtgaaa ttgaaaccga
     2341 ccgccgtatt attcgcgacc gaatagccaa gctaaaggag gaactgagca aaattgaccg
     2401 ccagatggct acacaacgta agaatcgagg gagcgtggtt cgtgttgcgc tggttggcta
     2461 caccaacgtg ggtaagtcca ccattatgaa cctcatcagc aaatcggatg tatttgctga
     2521 gaataaactt tttgccaccc tcgataccac agtgcgtaag gtggtaattg gcaatctgcc
     2581 tttcctgcta tccgataccg ttggttttat ccgaaagcta ccacatcagc tggtggagtc
     2641 gttcaagtca accctcgatg aggtacgaga ggccgacctg ctgctgcatg tagttgatat
     2701 agcccacccc aactttgagg agcaaatcaa tattgttaac caaacattac acgagattgg
     2761 cggcggcgac aagccaacta tacttgtgtt taacaagatt gatgcctaca cctggaccag
     2821 caaacccgac gatgacctca caccatcaac ccgcgaaaac attagccttg aggagctgaa
     2881 acgaacctgg attagcaaaa agggaaccca taccattttc atttccgcca agcaaaaaat
     2941 caatattgat gagttccgtg cgctactgta taagatggtt agagagattc atgtaacgcg
     3001 gtacccctac gacaatttcc tataccccga tattaccgaa gccaaccttt agcatttaag
     3061 tactcggcaa tttgaatggc attggtggca gcacccttgc gaagattgtc cgatactacc
     3121 caaagattaa ggcttcgcgg ctgtgaaaca tcgcgtcgga tacgacctac aaacacatcg
     3181 tccttgccct cggcaaaacg tggcattggg taatggttag tggcgggatt atcctgaacc
     3241 acaacaccag gtgcactacg caaaatttca tttacctcat tgatatcgaa ttcactttca
     3301 aactcaaggt taaccgactc ggagtggccg ccaataaccg gaatacgaac aacggttggc
     3361 gataccatga tagactgatc gccaattatc ttgcgggttt catcaatcag cttttgctcc
     3421 tcggtagtgt agccgttatc gagaaatgtt ccgccatgcg gaaaacagtt taagtcgatg
     3481 ggatgagggt acgccatatc ggcctcgtgc ccttggcgct cagcaaaaag ctggtttacc
     3541 gcttttacgc cggttcctgt aaccgactgg taggttgaaa caacaatccg tttaatacga
     3601 tacttaaggt gaagggggtt tatggctaca accagctgaa tggttgaaca gttcgggtta
     3661 gcaataattt tatggtttgg ggtaagaact cctgcgttaa cctcgggaac aacaagtgga
     3721 atttcggggt acatacgcca gcacgatgag ttatcgataa cggtagtgcc cacctcggca
     3781 aactttggag cccattcctt tgatgcggct gaacctgcag agaaaattgc aacggcaggt
     3841 ttttgcttaa cggcatcggc aacggagcaa acttttacct cggcatcctt aaactttacg
     3901 gttttaccaa ccgatttctc cgatgctgca acaattaact catcaacagg gaaattgcgt
     3961 tcctccagaa cacgaagcat aacactacct acgaggcctg ttgcccctac tactgccact
     4021 cgcataaata aatattttat aggtgattaa ttgaattttt tcgtaaattt tgattgtaaa
     4081 attatacaat agtttaaatg cgagtaacgg tcataacttt tttaacacta cttgccagct
     4141 tgacttttgg gcagcaagtg gtaaccttca attttgaatc gggtgattta tcggaatggt
     4201 ctcagaaacc cgaaaaccgt tggtcaatta gcacaaacag tccactctca gggagcgcct
     4261 cgctaaggca cagctttgac aatagcattg catacaccga taccatttat agggctttac
     4321 cggtgtggga tattgaaaaa ggtgatgtta catggcgatt tttgatacgt cacggttacg
     4381 acccatcggc atcgaatagc tggtgggttt acctgatggc aaacgatgaa attgcaaatt
     4441 caaatgaatc ggggtacgct gtaggtgtta acctaaccgg cagcgatgac ctattaaagc
     4501 tttggcgaat cgataatggt tcggctcagg ttatactaac ctctactctt aactggcaaa
     4561 ctcaaattgg caccgggaaa gcaggagcaa ttgaggttac cagaactttt gacggaaatt
     4621 ttaccctccg ggcatcagtt agcggtaact tcagcacctt aaccacctac ggaacagttt
     4681 atgattttag ctacaagaac tttaacattt tcgggattgg ttaccgttac tcatcggcac
     4741 aggacatgaa gctctgggcc gatgatattt cgttcatcta tcagccaatt aacccaaacg
     4801 accgtacaac agcagtgtta aacccatcgc aacaggttgt tccaaccaat ataatatcta
     4861 catcaacatt agaggaaaat tgcgtggatg ttttacggtt tcgtattacc gatgcgggat
     4921 ctggcgatgg tttgcctaca tatgttaagc gaatgacaat caaaaatccc gaccctgaaa
     4981 acgcatcgtg gagtagcctg cttggcggaa taaccctgaa gaaggatggg caacttatcc
     5041 caactcaaac cgtgagcata accgaccaac aggtaaccat tgaggtggat agctcgcttg
     5101 ccacagtccc cgacggccag tatacggagt tcacatttgg tgtttatctt aaggatagca
     5161 acattcccga taacctcaac cttttgtttg agatagataa cgaaaaccat ggttttcaat
     5221 cgagcctgtg ggggtcggga tttgctcaaa ccttcccggc caaggtgcaa tcgggtattt
     5281 tcaccattag ggtaaacgcc acacaggcta tgttccgcaa catatcgcct attgccatta
     5341 ttggcaaacc ctttggtctg gaagtagctg ctaccgatac taatagtaat accgatgccg
     5401 attatggagg tcagataacc ctgagccttt cagagggcaa cggaagtctt agctctgtaa
     5461 atgggcttag ccgagtagct atacagggca ttgccaaatg ggacagcctt ttatacgaca
     5521 ggtacggcag tatccgaatt atggccgctg gtgaaaacat aaacacggat attagcgatg
     5581 aaatagttat tggttacgat accacctcgt atgcggcagt tccgggaaat caggtgtcat
     5641 cgttcgatat cagttcaatg gctacaagtc cctcacaggc taatgagcta atgcgattcc
     5701 gcattaccga tgccggcagc gatggcgcac caacccacct catgaaaatc cgtttgaaca
     5761 gggcaaatac ggtaaatgcg gcaacgctaa acaaagcaat tgagggcgtt atggtaaaac
     5821 ttggggacaa ctatatctca ttttccagtg tcgatataaa aacttcaacc atcgatatta
     5881 cattccctcc tggcgcatta accatccccg atgggcaaac catcgaattg ggtatatggg
     5941 tgtacttgaa gaatagcgga atatccgata atcaaaccat tcaactttat atcgataagt
     6001 taaatccggg ttttgaggcc tacgatatag gaagccgatt cgctactaaa ttcccaacca
     6061 atattaactc ggcaatagcc acaattaggg taagtgctac aaaactaaac tttacctcaa
     6121 ctcccacccg tgtcggggtg aacgaaccat ttactgttaa ggttaaaccc accgatgcaa
     6181 acgggaatgc agatgttaac ttcgcaggta acgtggaatt gcttcttggc tcgggcagtg
     6241 gtgaacttga gttgatatcg gaaaatccag cgccaatcat aaatggcgaa actcagcttg
     6301 aagccaacta ttccactccc gggatattca ccctcatagc ccgaaaccca agccttaacg
     6361 atgctgtatc gggtaacatc accagtggcg atgccgatgg ttttgctgca cctttaattc
     6421 agcctaccga tacagttatt tttacccaaa ataacagcta cgctgcagca gcacaggaaa
     6481 ttattcgctt taaactgaaa gacccgggta gcaccgatgg tctgccgcta aatgttagcc
     6541 gcattaagtt actcgcattc aatccaaaca gtttaccaga gctagcccgt atggttcagg
     6601 gatttgtttt ggataatggc acgataacaa ttgcgccaaa atcgtacacg tttagcggta
     6661 atgttcttca aatcgatttc aacgagggtg acgttgtagt aaacgatacc gattcggcca
     6721 actttatcct caaagttttt ttaaaggaca gaaacctggt ggatgctttt cggtttcagt
     6781 tctatattcc atctagcaat cacggctgga ctgccttcga caatagtaca gcctttaaca
     6841 gcaccttccc ttccaccatt tacggtagac cctgccgcat tgaggtacag gccgacaggc
     6901 taaaattctt aacccagccc tttggggtta atcccaatga accctttagc gttagcgtaa
     6961 tggcctgcga ctcacgtggt aacgttgacc gggaatttca ggcttatgca gcccttgaag
     7021 tttataatgg tacgggcaat ctaattgtaa atccactcct acaacctctc gacaatggta
     7081 ttgccacttg gaacggggta aaactaacag ccaccggaac ataccggctt aaatcgtact
     7141 ttggctggtt ggccgatgcc atatcggaaa atatctactg cgggtacaac catacctgcc
     7201 cagttagtga gtactttgag agtattctgc ctgcatggag cggcattgaa aactggacaa
     7261 cctcgcgaac cgatccaata accggggaat actcacttgc ccatgccggc gaccccgcgg
     7321 caaatcaatc ggtactgagc attcctattg aaacaaccct gctggggaaa gcctctgagt
     7381 ggaacataac catcagaaat ggtaactggg atccatcatc ggaaaacttc ttttacctgg
     7441 cgtttgcctc aacacacgaa aatccgatgg atacagaggc tgagggttat gccgtaggca
     7501 taaacccatc aagtgggaac gataacattg cggtttggca gtttaaggaa ggtaagcgaa
     7561 ccaacattat tcagtcagct tttgactgga atgagaatga ccaagttaag attactgtta
     7621 ccctgtcgcc caacggaacc ataaaactat ggtttacacc aaaatcaaca gggcttaaaa
     7681 tctttggagg cgaagctaag attgcagtac ctcagtgcag ctacatggca tttgtatttg
     7741 cctacacagc gagccgcgct ggtcaacttt ggattgatga catgagcctt tgtactaccg
     7801 attttgcacc agttatcagc agcgcacggg cttcaaacct aaacacagta agagtaaact
     7861 tttccaaacc tgtttcaatt caatcggctc agaacgttca gaactacaag ctaaaaacgc
     7921 taacaaacca aaatatcggc atacaatcca taaggattga cggtaaccaa atggtttcaa
     7981 taaccacaca aaaattgcct tttgaaaagt taatattggt ggtaaggaat atcgaagata
     8041 tgaatggata cagcctcccc gatagcattg aacttggttt cacagcacag ggtaacatgg
     8101 gaaggttgat ttttaacgag ataatggcaa accctgtacc ctcaaatggc cttccggagt
     8161 acgaatacat tgaacttttt aaccctggaa ccgatactgt ttttaccgag ggctggaaac
     8221 ttactctaaa cgaaaaaact gttacaatgc ccgccgatac aattccacca aaatcgtatg
     8281 cgctggttgg cggaacgacc gccatgagta actttaatgt gtacggtaaa accatcgcca
     8341 ttacatcgtt tcctgcattg ctcaacgatg gtatgctact aaggctgtac gatcagcaag
     8401 agaatctgat aggatttgct aactacacaa aggaatggta caacgactca atccggaacg
     8461 tcggtggtta ctcgcttgag tgtatcgact attcaaacct tgccgagggt aagaacaact
     8521 ggcgtgcaag tatagcaagc gagggaggaa ctccctgtgc accaaactcg gtaatggcaa
     8581 taaatcccga tgtcaccccg cctcaactat gctatattga agtccccgat gctaaaacca
     8641 ttcgtttatg cttcagtgag gcaatggata gcctaactgc aacgattcac gaaaactaca
     8701 taacagagct tggtataaat aacataaaca tcggagaact ttatacgcgg gttaccattg
     8761 aactatcaga accgttagag gccgaaaaaa tttacgagat atccatttca ggtttaaccg
     8821 attttgcagg gaatgcaatg cccgacacag caatacgggt aggactacct caacttgcca
     8881 gtgagggcga tattgtgata aacgaaatac tattcaaccc ctatgttggc ggaaccgatt
     8941 ttgtggagct attcaaccgg tcaagtaaat gttttgacct atcgcagata aaaattgcca
     9001 accgaaacga aaccgacctt tccctaaaag accccgatag tgcatcacct ctaccctggc
     9061 tttttttccc gggcgattat gccgtaatta ccacaaatcc taaccaagtg acccagttct
     9121 actcctgcca aaacccttcg tcttttatca aaacttccag attagctgcc tttaacaacg
     9181 ataaaggtta cgtagtgtta ttaaattcag aaaatgaagt gattgatgaa ttgcgatata
     9241 ccgagaaaat gcacaacaag ttgcttaacg attttaaggg agtgtcgctt gaacgcataa
     9301 atcccagcat gccaaccaac caatcaagca catggcaatc ggctgcacag gcagccggat
     9361 ttgccacacc aacataccgt aactcacagt actctgagct aagcggagaa aaaagcaact
     9421 tcagcttaac acctgaaacc ttctcgcccg atggcgatgg ccgcgatgac tacctcctca
     9481 ttagctacca gctacctgaa cccggatatg ttgcaaacat cagggttttc aatgcaaacg
     9541 gcattgaaat ctacaggctt gcaaataata tgacccttgg aacctcgggg caactccgat
     9601 gggatggaac cgataccaaa aaccgacggg taaattcagg gatttacatt gtttactttg
     9661 aatacttcaa tctttcgggc gaggttaaac gcgaaaagaa aacctgcgtg gttggatata
     9721 ggtagaattt tttggtttta caaaataatt ggtgctattt tggaaaacaa aaaatgattg
     9781 gttatggaag gctatattga actccccaag tgtaccctaa ggtaccggat tagcggaagt
     9841 ggtccggcag tggtgctttt acatggttat ctggagagcc tcaccatttg ggaggagtta
     9901 accatggaac tcgaacccga ttacactgtc cttgcagtag acctaccggg tcatggcaaa
     9961 tccatttgca aggaggaggt gattggcatg gatttcatgg ccgatagcgt tagtgctgta
    10021 atgcgggcgg tgtcaattag caaagcacat ctagttggac actccatggg cggatacgca
    10081 agcttagcct ttgccgaacg atacccaaac tggctcacag ggctttgcct gctccattca
    10141 acccccaatg ccgatactca tgagaaacgt tcagctcgca tggccgatat caaacttgta
    10201 aaagaaggta aaaaggagca aattgttgaa aacaacattc ctcgcctatt tgccaaccat
    10261 aaccaacaga aacttatggg cgaaataaac catataaaat caattgcccg gaaaactagc
    10321 gaatcaggga ttgttggagc acttaacggt atggctcagc gtcccgaccg aaatcacatt
    10381 attgatcaag cgctattccc cgttacaatg atatttggca agcacgataa cctgatatca
    10441 atggaagtgg ctcaaaccct tgaagaacgc catagaaaag caagaacaat ttaccttgaa
    10501 aattcgggac acatgggctt tattgaagaa ccagaacaaa ctttctcagc aattaaatcg
    10561 atactaaagt aagatttaaa gaaacttaat tgttggatta aaggaaaccg ctccacgtat
    10621 tagaattatt aactttactg ctaatgcact taggataata cataaatgct aagcactaac
    10681 tggagtcaaa caaacatttt gcttatttac ctgcacttgt aactgttatt ttttaataaa
    10741 tttcgacatt gtttaaaatc gcagtttcct taaagttatc tttatagcat ggaaaaaagt
    10801 aagtatccat acggaaccaa gcagccccaa tttgataggc gttacctgca aatggcaaaa
    10861 atttgggcta aaaactcata ctgcaaacgc aggcaggttg gtgcgctaat tgttaaggag
    10921 cggatgatta tttccgatgg ttacaacgga acgccatcgg ggtttgagaa tatttgtgag
    10981 gatgagaccg gtaaaaccaa gccttacgtt ttacacgccg aggcaaatgc aataaccaaa
    11041 gtgtcaaagt cgagcaatag ctctgaggga gccacccttt atgttacatc atcaccctgt
    11101 atggaatgct ccaagctgat tattcaggca ggcatacgca gagtggttta ctgcgatgag
    11161 tatcacaatc ttgatggaat tgaactcttg aaacgtgcag gcattgaggt agtgaaaatt
    11221 gaaattgaag atattaatga attttaaaca agctgaaata ccatgagtta cccaaactcc
    11281 aggaaagata ttatttaccc agtagtcatt gccattgtgc tagttattgg agttgccctt
    11341 gggctgattc taggcggaag agttgagaaa cccaacctac tcgtttaccc acgcaccgat
    11401 aagctcagca atgttattcg ttacattgcc gatgagtatg tcgatactat ttcaacggag
    11461 actttagttg agaataccat accagcagtg cttaaaaacc tcgaccctca ctcggtttac
    11521 ataccggcca aggatttaca atcggtaaat gaaccactgg agggaggctt cgacggtatt
    11581 ggtatccagt ttaatatgaa caacgatact gttgtggtta ttaacaccat tcccggtggc
    11641 ccatcggaaa aattaggaat actggccggc gatagaattg ttaaggtaaa cgggaagaac
    11701 attgccggtg taaaattccc catggatagt gttcctaagc tgcttaaagg acctaccgga
    11761 acaagagtaa aggtaagcat taagcgaacc gacgttcctg atcttataga ttttgatatt
    11821 gttagggata aaatacccat ttacagcgtt gatgtagcct atatgcccac aaaggatatt
    11881 ggttatatca aaatcaacac ctttgccaaa accacctaca aggagttcct tgaggcaatg
    11941 gtaaagctaa ccgaaaaggg catgaccaag cttattctcg acctacgcag taatagtgga
    12001 ggctacatgg atgcagctat taacattgcc aatgagtttc ttcctgccaa caggttgatt
    12061 gtttacaccc aggggaaaaa ccgtccgcga caggaagcaa agtcgaacgg aaaaggattc
    12121 tttcaggatt tacccctcac cattcttatc gatgaattct cggcatcggc aagtgaaatt
    12181 cttgccggtg caatacagga taacgaccgt ggcattattg tggggcgtcg ttcgttcggc
    12241 aaaggattgg ttcaggagca ggtttacttt tccgatggct ctgcccttag actaactata
    12301 gcacgctatt acaccccttc ggggcggagc atacaaaaac cctataaacc tggcgatgag
    12361 gaatactttt acgaaattgg caaccgttac ctgcacggtg agtttcagaa tgccgatagt
    12421 attcagtttg ccgattcgct caagtactac accataggtg gtagagtggt ttacggcgga
    12481 ggcggaataa tgcccgattt ctttgttccg ctcgatacgg ttggtatcac cccttactac
    12541 caacaggttt cgcgccgaaa ccttatttac cgctacgcct ttaagtttgc cgatgagcac
    12601 aggaagcaaa cccgtgcact gaagagtttc gacgaggttg accgttacct caaaaagttt
    12661 aacatcctga ccgattttgt tggctttgcc aaacaaaatg gagttgagcc tgaccctaat
    12721 gatttagccc aatccaagct gattattgaa acccagctga aggcaaccat tgcccgaaat
    12781 atcatcgaca acgagggctt ttacccctac attcgatata ttgacgaaac cctgattaaa
    12841 gccattgacc tatgcgagaa aggtaactgc attttgccct taaataggaa ttagttgatg
    12901 gttgttcgta agaatattat catgataagc gctagcgaaa caatactctg tcatgctgag
    12961 cttgccgaag catctttttg ctcatttttc acttttagct ttgactgaga tccttcgcta
    13021 acgctcagga tgacaagtgg aggttgtttt ttgatagtga atcctttgga acacagataa
    13081 cgcagatttt gctgaattac actgagaaac accgtgtaac tttgtgtaac actgtgatac
    13141 tggtatagtt gatagttgat ggttttagca agtaaaaagg attcttaaga gaataatatt
    13201 tatattccct aattcaggaa ttattatatc gatttaagtt agctgaataa aaaaaaatta
    13261 ttttggtacc acaactaggg gtatactttt taaatggaaa tagacaccaa tcaaacagaa
    13321 gagattatag aggttggcaa ggctaacagg cttgcccttt taatgctctt cccaattgca
    13381 gcaattacaa tcataccctt tgtgattatt tatggttttg ggccacttgc caacggtgtg
    13441 atttttttga aaaatcacca gctgcaaatt ttggttgcat tcgttctgct tatattcgtc
    13501 cacgagggat tacatggtgt aacgtgggct ttttttgcaa aaaacggatt caagtcgata
    13561 cattttggga ttaagtggtc ataccttacc ccctactgcc attgcaacga accaatgaaa
    13621 cgcaaacact acatgctagg aggtataatg ccagggctta ttaccggcgt aatcccaata
    13681 ataatcgcca tggtaacagc taacggctgg ttattaattt caggtatttt ccttaccgca
    13741 gcagccggcg gcgatatcct agttctgata cgggtgctaa aatatccttc aaacctcata
    13801 tttatggatc atcctaagga gattgggttt atagtattta aaagctgaaa cnnnnnnnnn
    13861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncatatttat ggatcatcct aaggagattg
    13981 ggtttatagt atttaaaagc tgaaacagat taaacaatta cccatcgtcc cattttagtt
    14041 ttggggtaat gtatttaact attgcctaca ttcaccctcc gcaacaggaa tggttttgat
    14101 ttcattacgt gagactcacc gtaaactttt tctgctaact ttgtgcataa ttaaaaaaat
    14161 cacaactatg tacggaaaaa tcaaaaacga tttacagcaa cagcttgaga atatcaaggc
    14221 cgaagggctt tacaaaactg aacgcattat cactaccccg caaggagttg aaatagttgc
    14281 caacggcaaa aaggttttaa acttctgcgc caataactac cttggcctct cgtcgcatcc
    14341 tgcagttatt gaggcaggca aaaaggctat tgatgagtgg ggctttggca tgtcgtcggt
    14401 gcgctttatt tgcggaacgc aggacctaca caagcagctt gagcgtaagc taagcgagtt
    14461 tcttgggacc gaggatacaa ttctttactc atcggcattc gatgcaaacg gtggtatttt
    14521 tgagcccata ctaggcgagg aggacgcaat tatttccgac gaacttaacc atgcatcaat
    14581 cattgatggt gttaggcttt gcaaagctca gcgattccgc tacaaaaata acgatatggc
    14641 cgacctggag gccaaacttc aagaggccgc caactgccgt tacaaaatga ttgttaccga
    14701 tggtgtattc tcaatggatg ggattattgc tcaggttgac aagatatgcg acctagcaga
    14761 taagtacgac gccatggtaa tggtggacga ttcacatgct acaggttttg tgggcaaaac
    14821 cggtcgtggt actgctgagt actgcaatgc actgggcagg gtcgatgtta tttcaaccac
    14881 gtttggaaaa gcacttggag gtgcatcggg tggttgcatc tctggtcgta aggagattat
    14941 cgacatgctc cgtcagcgtt cgcgtcccta tcttttttca aactcgctca tgccttccat
    15001 tgccggagca acacttaaag ttcttaacat gcttaccgaa actaccgaac tacgcgatag
    15061 gctttgggaa aacactcagc tattccgtaa aggaatgacc gaagccggtt tccgtatagt
    15121 tgagggtgtt caccccatta ccccaataat gtttggccac ctacccaacg atgctaagct
    15181 atcgcaggac tttgctgcag ccttgcttga agagggtatt tatgtgattg gtttctatta
    15241 ccccgttgta cctaaaggta aagcccgcat aagggttcag ataagtgcag ctcacacccg
    15301 cgaacatatc cagtttgcca ttgaaaagtt cacaaaggtg ggcaagaaat tgggtgtgat
    15361 ttaaagaaat catcctatca gaaagattga tacctttatc aacctgtcaa tctcacagat
    15421 aaaaattgaa gggagtcttt ttttgactcc cttttcttat ctaaaacaaa ccatgtattt
    15481 gcgcatcgat cttattgata ataaacctga ggtcttcggg tctatcgggg aaattcagat
    15541 tgtcaacatc aattattagc agcttgccta acttgtagcc ttcaatccat gcctcgtaac
    15601 gttcattaag gcgctggagg taatcgaggc ggatattttt ttcgtaatcg cgtccgcgtt
    15661 tctggatttg ccttaccagc gttggcactg tggcacgaag gtaaattagc aaatcgggcg
    15721 gtttaacaag cgaggtcata aggttgaaca gggaaaggta gttgttaaaa tcgcgggtgc
    15781 tcatcaatcc catgtcgtgg aggtttggag caaaaatatg ggcatcctcg taaatggttc
    15841 tatcctgaat aacatcgtga ccgcttcggt gaatctccac tatttgacta aacctgctgt
    15901 ttaggaagta aatctggagg ttgaagctcc agcgctgcat atcctcgtaa aagtcgttaa
    15961 ggtatgggtt ttcatcaaca tcctcgtact ggggtttcca cttgtagtgc ttggccagta
    16021 aacccgtaag ggttgttttg ccagaaccaa tattacccgc aatggctata tgcataactt
    16081 aaaataatta cttaaggttg gtaattcacg ttttcgtttc taaagataca aattggattt
    16141 taatcggcaa ggtaaaacag cttgtagatt gatgaatgtg tcaaaaatgt aaataaatcc
    16201 tatctatttt gatgactact tactttttag ctggttatga atggcaagca gctcatcgat
    16261 atactcgcga gtattcgaaa gccgaggaac tttattctgc ccacccaatt taccgcgctg
    16321 gcgcatccac tcaaagaatg tcccctgaac agcctgcgta accactggaa aatcgagcgt
    16381 tacatttttg gcgcgttttg cctcatagtc ggaattgacc tcacaaagcg ttgagtcaag
    16441 aactcgagca aactcctcga tgctgcttgg ctgttttaca aactcaatga accattcatg
    16501 gcatccctta cggtcagttc ccatgtaaac gggagcagcg gtgtactcag caaccaatgc
    16561 tccggtagcc ttacaggctt tttcaagtgc acgctcagcg ttttcaacaa taacctcttc
    16621 gccaaaaaca ttgatgaagt gacgggttct gccagtaatc ctgattttat gaggatgttt
    16681 tgaggtaaag gtaaccgtat cgccaattag gtaacgccaa aggccagaat tggtggtaat
    16741 gaccatggca taattccttc caacctcaac atcggcaatg gtaagggctg taggattagg
    16801 tttggtaagc tcatcgagag ggacaaactc ataaaaaata ccgtagtcga gcataagcaa
    16861 catgctatcc gacgcagggt cgtcctgaat ggcaaagaaa ccctcgctgg cattatatgt
    16921 ttccatgtag tgcatgttag gcgatgggat aactttttga aactggtccc tgtatggggc
    16981 aaaacttact ccgccatgca tgaaaagttc aagatttggc catatctcaa gcaagttact
    17041 tttgcctgtg tagttcagca ggtgcttaat cattaccaga ttccatgaag ggactcctgc
    17101 caggctggta acattctcgt taagggtctc gtgagtgagt ttctccagtt tctcttccca
    17161 atcgggtatt aatgccactt tttggcttgg ggttctgata aactcagccc accagggttg
    17221 attttcaatc agaatagccg ataaatcgcc gtaaaaggaa ttcatattaa agttatcaac
    17281 ctggtggcta ccgcccaggg taagtccttt tccgcttaac aaattgtttt caggataaag
    17341 gttcatgtaa atggcaagca catcgcgagc ccccctgaaa tggcaatcct ccaacgcctc
    17401 gttgctaact ggaataaact tactcttgct ctgggtagtt cccgatgact tggcaaacca
    17461 cttaatatcg gttggccaaa gcaacttttc ctcgccctgc ctcaaccgtt caatgtatgg
    17521 cttaatggtt tcgtagtcaa caaggggtac tcgcttctga aattcttcaa tggttctgat
    17581 gccatcaaag ccatatcgtg ctccgtattc cgtttttgag ccttgtttta aaagctcaaa
    17641 aagcacatcg cgttgcacct caaaagggta tttccggaaa agatcgatgc tgtataagcg
    17701 cttagtgttt acccagttca ggatagagtt gagtaaaggc ataatcaaaa aattatcaac
    17761 tcaaaactaa gtcattttgc ccaaaaatca aaagggctaa agcttgaagg taatacccac
    17821 gccaaaaatc tccttaaact gaacacgtgg cccactgcta tcaattacac catcgccgtt
    17881 gttatcctta ctaatcttaa ccttgtcatc gtagattagc tgggttgcca ggttaacatt
    17941 gatgtacttg ttaaccttca taattacaag cgtttcccag ttaaccacaa tgttttgtgg
    18001 gttatcaagg tagttggaga ataggtcgag cttagttgta aaggtgaggt ttttgagcca
    18061 ttcggcgtta aagtcattct tggtgtaaac cactctcacg taaccgccaa tttcctctct
    18121 gagtgtttcc ccttttttta cgccaaatgc tcctgcattc gatagcatgg tgtccgtaac
    18181 aaaggttagc tttccggtta acggtgctgc aaaaacactc aggtgggtgt tcggtttata
    18241 atccataccc aatgccccaa ccaggtatgc cggagcaaaa aagttcgaaa ttttcagacg
    18301 ggtactatcg gttgggtatt tataaccggt agtgaattgg gttttaatgt tcagcaaaac
    18361 agcatagtaa aagtttttga aggcctcacg tccaaatttc gacattaagt caattttatc
    18421 gtcggttttt ttggtgtact taactccctc ctggctcaga aatccgtagc ccaagtcaat
    18481 agtgttatcc caagcagttt tgggactttt atagctaaca aaccctgtaa aaacggtatt
    18541 tagtgcaaat gagctttgtc cgccggccgc ccaattggtt aacgaagtct ggttggcatt
    18601 aattcccacg gccagccctc tcttccatcc ggaaagcgaa tcggttttaa tctctttaag
    18661 ctgattttca gcgtcggtta cctgagcgtt tacagttata gtaagtatcg ataacaaaat
    18721 agtggtaagt aatttagtca tggctgcatg aattattcat taatttacgc taaaatacta
    18781 attattatta tgggaaaata ttcatttcaa agctgtaact ttgcgggcta attttttggg
    18841 gatgaagggt atcagaaaca ttgcaattat tgcgcatgtg gatcatggga aaaccacgct
    18901 ggtggataaa atgatcattc agggtaaact ttttaaagat catgagaacc cgggcgaact
    18961 gattttggat agcaacgacc tggagcgtga gcgtggtata accattctgg ccaaaaacgt
    19021 atcggtaaac tacaagggtt acaaaatcaa cataattgac acccccggcc acagcgattt
    19081 tggaggcgag gttgaacgcg ttctaaacat ggccgatggt gtgctgcttc tggttgatgc
    19141 ctttgagggg actatgcccc aaacccgttt tgttcttcaa aaagctttag ccataggctt
    19201 aaaaccaatt ctggtgatta acaaggttga taagccaaac tgtcgccctg aggaggttca
    19261 ggagcaagtt tacgaactaa tgttcagcct cgatgcctca gaggaacagc tcgagttccc
    19321 taccatatac ggaagcgcca aacagggatg gatgagcaac gactggcgga agccatcaaa
    19381 cgatataacc cctctgcttg attgtattat tgactatgtg ccagctccaa aacagctaaa
    19441 aggcacgccc caacttctta ttacatcgct tgactactca aactatgttg gccggattgc
    19501 cataggcaag ctgcacaggg gaaccctaag ggcaggccaa aacgtatcgt tggcaaagcg
    19561 aaacggacaa gtaatgcgta caaagatcaa ggaactactc gtttttgaag gtcttgaacg
    19621 caaaaaggtt gacgaggtaa gctcaggaga tatctgtgcc cttgttggca ttgaagggtt
    19681 tgacattggc gataccatag ccgattacga aaaccccgag ccacttccta ccattgccgt
    19741 ggatgagcct accatgagca tgctcttcac cattaataac tctccgttct ttggtcgcga
    19801 tgggaaatac gttacctcgc gtcacctaaa ggataggcta atgcaggagc tagaaaaaaa
    19861 cctagccctt cgggttgaag aaaccgattc ggccgatgcc tttaccgttt acggtcgtgg
    19921 agtactccac ctttccgtgc ttattgaaac catgcgtcgc gaagggtatg agcttcaggt
    19981 tggtcagccc aaggtaatta ttaaggagat agatggagta aagtacgaac ccattgagta
    20041 cctaaccatt gatgttcctg aggatgtatc gggaaaggct attgagcagg ttacccgccg
    20101 caagggtaca ctaatctcaa tggatcgccg ccacgaccgt gttcatcttg agttcgagat
    20161 accctcgcgc ggtattattg ggctccgtaa catcattcta acggctactg ccggcgaagc
    20221 catcatggcg caccgcttta aggcatttga gccctaccgg ggcgagattg aaacccgcac
    20281 caatggttca ctaattgcca tggagagcgg taccgcttat gcttacgccc tgggcaagct
    20341 acaggacagg ggtagattct ttatcgatga gcatgaggag gtttacacag gccaagtggt
    20401 tggcgagaat aaccgctcag gcgatattgt ggttaacgtt accaagtcca aaaaactaac
    20461 caacatgcgt gcttcgggtt ccgacgaaaa gatattctta gcccccccca tcaggtttag
    20521 ccttgaggaa gcacttgagt acatccagga ggacgaatac gttgagatca cccctaaagc
    20581 catacgccta cgaaaaatat acctcgacga aaacgagcgt aaacgtaggg ctaaagcata
    20641 gcggaacggt acttttttga tgaatatttt taactttttt tgggttgaat tgatttttta
    20701 taactttgca cgttgatttt gtgagaaagt ggcaagcatt ttaggcgcat attcaattag
    20761 ctacaaggga cttaagcttg gcaaacactc gtttgatttt gaaataaaca acgatttttt
    20821 tgctgagttc cctgagggtg aggttaagga aggcacgctc acagcaaaag ttatgatgaa
    20881 caagcagaat aacctgcttg aatttgacat agtgataaaa ggaaaggtaa aggttacctg
    20941 cgataggtgc ttggaacaat tcttcctgcc aattgagtac aagggttttc ttgtggcaaa
    21001 aattggaaac gaggaggttg aggatcaagc cgatataatc ttcctaaccg aggatgacca
    21061 tgaggtaaac cttgcccagt acctgtacga gagcattcac ctgagtctac cgctaaagcg
    21121 gtaccatggg ttaaaaggat ccagtattga ggattgcgac caggaaatgc taaagtacat
    21181 caagtttgaa gacgatgacc aggttgaggt tgaccctcgc tggcaggctt taaaggattt
    21241 gttgaacaat aataactaag ttggactata taatttgttg aaaaatggca catccgaaat
    21301 cacgaatctc taaacagaga aggaacaagc gtagaactca ctacaaggcc gaagctccaa
    21361 ccctttcaac atgttcaaac tgcggagcag cagttgaata tcacagggtt tgccctgagt
    21421 gcggctatta ccgtggtaag ttagcaatcg agaaggcagt taacgcctaa aaatgaattt
    21481 cataaacccc tgcattgatg attagaattg gcgttgatgc aatgggcggc gattttgcac
    21541 ccgatgctgc tgtgtcgggt gcagttttag ccctcaagca tcttaatgcc gatgagcgca
    21601 ttgtgctttt cggtgaccag caaaggattc ttgaaatcct tgcccgtgaa aatgcaaatc
    21661 ctaccgattt tgatatagtt cacacctctg aggtgattga aatgggcgat cacccatcga
    21721 aagcgtttgc caagaaaacc gattccagta tagttaaagg ctttatggct ttggctgctg
    21781 gcaaaattga tggttttgcc agtgctggca gcactggagc catgatggtt ggcgctttca
    21841 tggtgataaa gcaaataccc ggtgttatac gtcccggaat agccggtttt atccctgttt
    21901 ccgaaagtaa gcttaatgtt attctcgatg tgggtttaaa ccccgattgc cgtcccgacg
    21961 tactttacca gtatggtaaa ctgggttcaa tttacgccca gcaagtgctt ggcattgata
    22021 acccaagggt tgccctactg aatattggcg aggaggagga gaaaggcaac ctggtaacca
    22081 aaagcgcctt tgagatgatg aaggactcaa ccgattatac ctttgttgga aacgttgagg
    22141 gccatcacct attccacgat aaaaaggccg atgtggtagt ttgcgatggg tttgtgggta
    22201 acgtagtact caaggaggct gaggcgttct accacctgct taagaaattt agccctgaaa
    22261 ataagttcat tgagcgtttt aactccgaga actacggtgg cacgccagtt cttggggtta
    22321 ataaacccgt tataattgcc catggagcat cgagccccgt ggctataatg aatatgatta
    22381 aacaaacccg cgaggttata gtatcggacc tttgcgaaaa aattaaacaa gcatttatgt
    22441 agctatggga aaaattactg ccgcaattac tggcgttgga ggatatgtgc cagagtacat
    22501 cctcaccaac gaggagttaa gtagaatggt cgataccacc gatgagtgga taatgacccg
    22561 tattggtatt aaggagcgcc gaatactaaa gggcgaaggg cttggaactt ccgatatggc
    22621 agttcatgcc gttaacgagc tgctccgtaa aaccaacacc caccccgatg aggtggacat
    22681 gttaatttgc ggcgtagtta cccccgacat gcagttcccg gctaccgcta acattataag
    22741 cgataagtgc gggattaaga atgcttttgg ttacgatatg aatgcaggct gttcaagttt
    22801 cctgtatgct ttggttacag catcaaagtt tgtggagtcg ggttcacata aaaaggttgt
    22861 ggtggttggc gccgacaaga tgtcgtccat aaccgattac actgaccgca ccacctgtcc
    22921 catttttggc gacggagcag cagctgtgct cctagaacct aaccatgatg gctttggcgt
    22981 aatagacgcc aagatgcagg ttgatggctc aggacgcaaa caccttcacc aggtggccgg
    23041 tggttcatgc taccctgctt cgttcgaaac tgtagccaag cgccagcact tcatttacca
    23101 ggaaggccaa cccgtgttca aagctgctgt ttcaaacatg gccgatgtgt cggttgagat
    23161 gatggagcgc aacaatatca agcctgagga tttggcttgg cttgttccac accaggcaaa
    23221 catgcgaata attgaagcaa ctgcaaatcg catgggaatt gacaaggata aggtgatgat
    23281 taacatacag cgctacggca atacaaccag tgccacaatc cctctatgcc tttgggaatg
    23341 ggaatcgaaa cttcgcaagg gcgataatat aattattgca acctttggag caggttttac
    23401 ctggggtgca gcctatgtaa agtgggcgta cgatggtgat aaaattgcaa agtaaaatat
    23461 tgttttcaac aaaaaagccg ggtttagccc ggctttttta ttatttggtg ttagagtcta
    23521 acattactga tgcagtattt cctgaagttt tcgggtttta tcaattacat cttctgttaa
    23581 gcgatgaacc gaagtggtag cgcctgatat tgcatcaatt tctttgccta ctgtcagcgt
    23641 attttttccg ttgtagccaa caaattgatt taaccaactc tttagggtga tttcctggcc
    23701 gtgagtggcc tcaaagctat aaatctgaag tgagagaatt ttacccgacc tgtcgaaaag
    23761 gataaagtaa tcaaagtact cagacccatt ggcattaaca ttatcatcgg aagccttgca
    23821 accttcggtt ctgcatgtaa atacccttcc tacataggaa aatgaaaccg ggttagcatt
    23881 ggtataagtg aagaatttac ctgttttata ggccataatc tccttggcaa tgggcgtttg
    23941 actcccgctt attctcttaa cctccctgct aatctgtttg ggttcaaagt tgatttcaga
    24001 tccaacctgc aagcttaata aaacccaaaa aattgccatc aacagtttca tacagcaaga
    24061 ttaaaaattc actcctatac ccgcattaaa tgtcttgcta aaggagtttt cggctttgga
    24121 tttataaaac tgtaaatctg ccttaagtac tactcctttt tctaaagcgt aggtcaagcc
    24181 tgtggtgatg atagtattac tataactttt atttgttaca actggtgcct caactgaatg
    24241 gtgagtgttg taagcctcat aacgaataaa gggcattaag cttttcttag tgtcagccaa
    24301 cgtttgaaat atgtcatacc caacctcaac gtaataaccg agcatagcac ttcctaaatc
    24361 gttaagcttg gtaccagtcc tggtaaatac attatactca ttggtattag aaagtgaaat
    24421 gtagtaaagt tggccagtaa gcttaacgcc tttgaaattg taacgagtat ctaaaccaac
    24481 cattgatatt ccaacaaccg atgagtcggc cctttggatt tttgcgaggc tatccttact
    24541 gaggttcgag taaagtttgc tattcgattt tccaaaatag cccgataatc caacattaag
    24601 atttttaacc ccataaaact ccagtttacc tgtaaatgca ggttggttca tgtaagcctt
    24661 tgaggctttt tgacggccct ccctaagtcc cttactaccg gaaaacatag cattggttcc
    24721 atcgtaacca tttaatccgt ttactacata gagctgatac ctggttttgg cctgagaaac
    24781 atttccttgg aagcctagcc ctacctcgcg ctaggttgtg ggcgcaattt tgttgtcgat
    24841 aatcggacgt tcaacaccat tgaaagcggt tggttcatgg tactcattaa taatacccat
    24901 tggcacaagc agtactccag ctctaaagtt tgcatacctg tttaacctgt attgaataaa
    24961 catttgctca acccaaacct ccctggcata ctcaatttca acctcggtta caaactgcgt
    25021 tttactggaa aaattgtaac caaagaacag caccatacga tgagcatcga gcgtacctag
    25081 ggtgtgggtg ttgctcttta acggttgatt gtagtgaacc tcaccatatc ccccaatttg
    25141 aagccctggt ttactgttca ttagtgtttg agcagtattt tgctgctgat tgggaatggt
    25201 gtcggtttgg gccttaagac caaacgaaat cacaagtaaa ccaaatacat acaaaactct
    25261 tttcataacg gcattattgt taattgtttt cgccgcaaat ttatttagac taattctatt
    25321 ttatgcaaaa atagtattga tgtttatcaa tcaactctat ttagaattgt gactccaata
    25381 acatcctgaa cttgttaaag agcaatattc taagtgtgga gagtggtggt gtttgttttt
    25441 taaggttaaa ggataaaaat ttaaaatttc agatttcaga tttcagattt cagattcatg
    25501 atggaaggtg gtttcttaag ccatcaacca tcaacaaaca actatcaact aaattggtat
    25561 cacagggtta cacagtgcaa cacgttgtta cacagagtgg caatcacgaa caacgataca
    25621 cgaacaacga acgccgtttc cagcccagta ggggcaaatc agctaagctc agggttttaa
    25681 ccctggttat tgtggttttc aaattattag gcgatgcacg caatacatga gggaaaggtc
    25741 attggtacat aaacatttag tccttacggg tctgaagcgt ttttcgtgaa attattgtca
    25801 tgttgaacta agccgaaaat ctcatgggaa ggattagaga tccttcgctg tactcaggat
    25861 gacaaatgaa gtcattcttg taagttgttc gttgttcatt ggataggacc tcaccccagc
    25921 ccttttccct gagagggaat ggagcggaaa aagcaagcag aggcataaaa gcctgacccg
    25981 gaaatgggtc gggggtggaa cgtggcggtg ggcttagtaa gcgataccac atgagtcacg
    26041 ccgaaggcgt ggcgaattgt atcgcgttgc agttccaccc acggggcacc catcgggtcg
    26101 ggcttttcag ccttgatttt ctttggttct ttcttgtatc aagacaagaa agaacatgta
    26161 aaccactctt tgccatactt ttctgttgcg atgcaacgtt tcagtgttgc tctttggaaa
    26221 aattattgcg tgcatcgcta ttctagtggg ttggcatttt ggttacaaac attacgcccc
    26281 tacggggctg ttcaagggat acatgaagga gattcctcga ctagctcgga atgacaacac
    26341 cattggctcc ctcactcact ttatcatgtt gagcgcaagc aaaacatctc atggggtcga
    26401 ttagaaatcc ttcgctgcac gctggatgac aggtatgtat attttattaa ctaaacgaca
    26461 ttgatataac attataaatt tccctggcta aaagtcattg attccggttt tggtttggaa
    26521 attatgctta atttaagaat taaaggggta aagcattggc tattacccct taaatgaacg
    26581 atgaaataaa aaatctattt ttgcctaaaa ataccaagta ccccaattat acttccagcg
    26641 tcagaggtta atgggtataa gcttacagag cctttcatct cctgaccgtt ggcaaaggtg
    26701 agtgaaatct tgcggtcaac taccgggctt ttcttgcgag caacatcctc ggttaaatcc
    26761 caaagtttac gggcagtatc ctcaggcaag aggtcaaact cgtttaaacc aattgcctcc
    26821 tcaacggatg ataggccaag tagcgaaact aatgaattgc taatccggcg gtagcgaccg
    26881 tcggcatcct tctggaagat gaggtcgctt gaattaagaa gtaagctttc aagaatagtt
    26941 ttatcctcgc gaagttgggc ttggattgtt tcctgctcgt gacgcttgcg ctccatctcc
    27001 tcctgggtag catgtaactc ttccatgttc tggcgcatct cctcttcctg cgcccgcatt
    27061 tcctcagctt gttgttgggt ttgggttaag agttgagccg tacgcatatt tattttaacg
    27121 cttgcaaggg ttgcggcaat gctagcagct agcttctcaa taaaatcaat ctgatatttc
    27181 tggtatggtg tgaaagtggc tagctcaact actccctgaa caatatcgtt tgctataagg
    27241 ggtactataa gcacacaacg cggattttcc tcgcccagcc ctgaagttat tttgatatag
    27301 tcgcgcggca catcaaccat taaaatggtt ttcttctcaa cgtagcatcg tccaacgagc
    27361 ccctcgttcg gcagcactgt tttttccatc agcttacgac ggttatatgc gtagcatgca
    27421 gtcatctcaa gaactgtatt gttggggtcg ttctcgttaa caataaacat acccccctga
    27481 attgaaccgg tgtagcttac cagattcttt attatcaggt aggacagttc ggtgatattg
    27541 tcgttatgct ggcggagtac ttcaccgaaa agcgccatcc cttgtgtagc ccatatctgt
    27601 tgctgctctt cttccttgcg tttttcctcc tgctcgcggg ctttaattaa gctccgctgc
    27661 atctcggtta atgcttttcc tagcttgtcc ttctcactga gcaacttgaa ctcggtatcg
    27721 agttttccct caccaatctc gcgggcaaat tgctcagtat agtttaatcc gtcaattagc
    27781 ttgttagccg aacgcgacat ctcctcaagc tcatcgccag tatgcaggta tattttttta
    27841 ttcacatcaa tatcgccttg ggctatactg ttaagtaact cagtagtacg ctttataggt
    27901 tttgaaattc gagctgagat ataccaaatg gcaaaagctt gtaggataat gccgagaata
    27961 aaaacaagaa ttgtggagaa cactgaccgg cgagcgtctt taacgatttc cgacatggga
    28021 atggaaagta taagccccca tgagttgggc gattcacccg gctgcaatat gctaagtatg
    28081 ttgaggtact ccattccgga attgtaggag aaaaagaaat ggttttgctt atcctttacc
    28141 ttttgggtaa gcgcaaactt cgactcaagt tcagggtaaa tattagcaaa gtattccttt
    28201 gcgttttcag ggtttgaatc ggcaattata cgaccatctc cagcaatcag gctaacattt
    28261 gtgcccggat agggttttat tttttcaata tcggtttgga acttctccag cgagatatca
    28321 acgccgccaa ggccagcaaa tttacctttt acccttaccg gtacactaag gctggttgcc
    28381 agcacttctt taccaccata ctcaaaggtg tatgggtcga gcaacatctc acagttgctg
    28441 gtttttgatg cgtagtagtt gctggttaca atatcacctg taatattttt atgctcaata
    28501 tcaatgccta cctgtccgtt gctaaggtac gctgttactg agcgtcggcc gtaatctttt
    28561 gtataacctg gacggaacgc agagtattca attgaatacc ataccgtaag gtatcgggga
    28621 ttgtttgaaa cctgatttcg cagaatattg aaaaagatgg aatcgagggt aacagtatcg
    28681 aatttggggt agatgtaaag ggcatgagcc agggctcttg aaaaacccat atccaaatcg
    28741 agttccgatt ttaatcggaa agccgatttc tgggcttcgg cctgtgcaat cttaatggca
    28801 ttatcgcgag caatactgtt cacccttatg ctaatgaatg agcctattac aatcattatt
    28861 gctgccgagg tggttaaaac ccaaagcaaa agcttggcac ctattcgcat ctttattttg
    28921 tcggcactaa tgttcaattt tccaattctc atgttgccta gtatgtttta cgattacaat
    28981 tttaatggta aaaccgcaca atcgtttgca atggttaaaa atacgaaaat gttttagcac
    29041 tatacaattt tttcaaaatt tcgtaatagt tcatcagtaa aaattagccc atcaaatttt
    29101 ttttggtaaa ttgctccttg tagaacagga cagcactatg agaatactga atattaaaaa
    29161 gtgcatcact tttttggtgt ttttggcagt tagtattttt gcgggtttac atgcacagga
    29221 aatcgatagc cttttaagtc tatcagagca ttaccggaca gttaaccccg attcggccgt
    29281 tagcgttgct aggcaagctt tggcaaaggt cgatgcagta aatcaacccg atattgcggc
    29341 ccgggtttac tttagtttag ggtatgccta ctacaatcgc aacgactttg agcttgctct
    29401 tgaacatttc aataaatcgt tgatgcttgc tgagcaatcg gataatgttc agcttaaagc
    29461 gttactacac aaccgtattg gtaacgttta tcagctaaaa agttactaca gccaagccat
    29521 tgatgagtat aaaaaagcgc tggactacaa ctacaagata ggaagcaagg tagaaattgc
    29581 ccgcacattg gttaacattg gaacggtgtt tgcaattacg ggtaatttct caattgcctt
    29641 gcagtacatg ctcgatgccc ttgaaatttt tgagcaatcg ggcgaaactg agggtatggc
    29701 ctggacatcg ttaagcattt cgcgcctttt tagccgattg gagattaacg aaagggcttt
    29761 gcagtatgcc gaaaatgcct tgaaccatta ccgcaggctt aacaatcaaa ccggcataac
    29821 cctggcacaa accgagttgg ccaatattta ctaccatacc ggcatgcacg acaaggcgct
    29881 cgatttactg ggtaaggtaa ttgatgagaa ttcacgcacc cgaaacatac acgcacaggc
    29941 agctaactac ctgctgatgg gaattatcta ctttgataaa aaacagtacc agcaatccga
    30001 atactaccta aacctatcgc tattgctgaa acagcaggta aacgatagta ttgatatggc
    30061 aaagcttaac cgatacatgg gtgaacttga gctgcaaagg ggaaactttg ccaaagggat
    30121 tacccttctg aataaagcgt taaacgatgc ccaaaagcaa aagattacta ccgatttacg
    30181 cgatatatac tttagcttat cggacgccga acgcaggaaa ggtaattaca aaaaggcata
    30241 cgagtacttt accagctatt cggcacttaa agatagcgtt aacgccagcc aaatagccaa
    30301 gctggagatg cagtacgatt ttgagaaaag ggaaaaggaa caggagttaa tctcgcgcca
    30361 gcgcgatgcc atgcagcaag ccaggcttga acggcagcga atggcaaccc tgcttatttc
    30421 aatagcttta gttttgagcc ttgctctggc agctgtagta ttcagattct accgcgaaaa
    30481 aaagagaacc aaccaaattc ttatccagca gaacgctgag atattaaggc aaaagaacga
    30541 aattgaaaac cagaaacagg agattgaggc ccagcgcgat ttggccgaaa ggcaacgtga
    30601 ccaaatagcc gaacaacaaa agcaaataac cgatagtatt aagtatgcga gccgtattca
    30661 ggctgctgtt cttccgcgta gccagcaaat ctccacactg ctgcgcgatc actttgtgct
    30721 ctaccaaccc aaaaacattg ttagtggcga tttcttttgg gtaacacagc taaccgatgg
    30781 tagaattgcg atagcagcag ccgattgcac tggccacggg gttcccggag cctttatgag
    30841 tatgcttggc attaccctgt taaaggaact aaccgcaaca caacattttg agaatgccgc
    30901 cgatatcctt ttgcgcctcc gccgccttgt tattgcctcg ctcaacccag ccgaaaccga
    30961 taacgattca cacgacggta tggatatggg tatgctcata gttgaccgaa aaaggatgga
    31021 ggcccattac gcaggcgctt atttcccttt gctactcata agggattcct ccgttcaggc
    31081 ccctccaacg cactcaaacc ctgttatagc tgacggaagt actctttacg aaattaaagg
    31141 cgataaaatg cctattgggc atcacattat tgaggaacga atgtttacta accacacctt
    31201 tgctatcttt gaaaacgacc tgttctacct gctttccgac ggatactccg accagtttgg
    31261 cggccccaaa aactcaaaat ttatgatggt gaaccttaaa aagcttctgc ttgatattca
    31321 tcacctgcca ttaaataccc agcaaagtat tctacaggaa aaatttgatg agtttaaggg
    31381 taacgagaaa caggttgatg atatacttat tatgggattt agggtttgac agccaaactt
    31441 ctctcatttc ataaaattat caacctaata gacgggtttt tcctgatacc ctcttcttat
    31501 aaaccgagat tagtcaatag aaacacaata aaatatttac acatcttgtt tctactgact
    31561 tatcgaggta atcccgactt atcatattct gcttattccg gatcattccg actgattgct
    31621 taaaaattag cccggttgaa tttgttaatt catgtcattt ggtttagccg ttcacttttt
    31681 acaattcctc taacttcctc taactccatc atattctgct tattccgact cattcctcta
    31741 aaattagtct ggtttaattt gctaattcat ggcactttta ggtgcctata actgctgaat
    31801 ccatcatata gcacagcaat aaatcgcatt aaacttttct ttcaatttat cgtaaagtgt
    31861 tttatgtttg catgataaag tccaactatg caaagctcaa attttttaca tagagtttta
    31921 atactttgtt ctgcagcttt gcttttgcaa tatccattct cagtattttc ccagccaaaa
    31981 tccgatagta ttgctgttag ggccttaatc gattctgctg ctaatgcaaa atcgccacag
    32041 gtaagaatag cgtttgctcg cgaggcatac cataaggcaa aagccacaaa tagcaagcac
    32101 ttgcttgctg atgcaactca ctcaatgggt aacgctcttt ttaggctaaa cagttacgat
    32161 gccgccatcg atagcctaca aagggcattg gttctttaca atattgttgg tgatagtgca
    32221 ggaactgcaa aaacttacta taccctgggt tcttgctatg ttcggaaatc gaactatgta
    32281 aaggcaattc aactaattga ggaaagtgta aagcttgcta catcgattaa cgatagtgca
    32341 ttgctagcaa attgctataa ctcacttgga attgtttact acacccttga gaacttcacg
    32401 gcatcgatcg atttctttga gaaagcagca aactactacg aaaaagctca cagctacaaa
    32461 aactatgtaa gggctaaggg aaatgtaggt ttaagcttaa ccaaaattgg gaacttggaa
    32521 ggtgcaagac aaatcttttt ttccacactt aacagctatg gcaagtacat ggattcgcta
    32581 atgcttgcct cgttttacga taacattggt tttttctatg agagcttggg taaggttgat
    32641 tccgctttgt actaccataa tcaatcatta accataagca aaacgctcag ctccactcgc
    32701 ggaattgctc taagcaattt ggcaattggc cggtgttata ctggtatagc gcaatttggg
    32761 cttgcaatta aatacttgct tgaggctaaa cgcctagcca aactcaatgg cgatgaagat
    32821 ctttcagctc aagctaacag gcagctatca acctgctacg aggcaaccgg cgattttaag
    32881 aattcccaaa aatgtttaaa ggaatttttg gagtataccg ataaaacatt caccaagaag
    32941 cttataactc aggttgcaca gctacactca atgttgcaga ttgagaagca ggaaagggaa
    33001 aaccagcagc tacaaatgca gctggagctg aaatcgttaa acgaacagaa ggataaaaag
    33061 ataattactt tgtacacaat cttttcaatt cttttgctcc taaccctcag cgtaattgtt
    33121 tactttgcca tacagctctc ccaaaagaaa aagcttttag agcatacaaa ctctcagctg
    33181 ttcaagttca ataatgaact cgattccctg gtaaaacata gaaccaggga gttgaataat
    33241 gctattgaca aaataaggga gcttgaaaga attaaatccg ctttcatggc aaatatcagc
    33301 catgaaatcc ggacacctct gaatggaata ctaggactct cctactacct ttcggcacca
    33361 gaatgcactg ctgaagagcg ttcgcagcta ggcgaacagg ttaagcgttt gggcaataaa
    33421 cttgtaagaa tagttgacga tatccttgaa ctttcaaaaa ttgaaactaa ccaggtaaac
    33481 ctactgctat ccgaggtcaa cctaaatcag ctgctcgatg atgtttacaa ggagtttgac
    33541 gccattgctg aatttgatga taaaaatctc ttttttaggg taaataaatc gttgcccgac
    33601 gatagggcaa tagtaatatg cgattactac cgtttgcgta gcattattat tcatctttca
    33661 gagaatgcct tcaagtttac ccaccagggt ggggttgaaa tgggttactc cataaacgag
    33721 aactcgttgc tatacctata cgtaaaggat acaggggttg gcataccaaa cgatatacag
    33781 aagaaagttt ttgagcgttt ttataagcac ttagatgatt ccagcccaat tttttacgat
    33841 ggtctaggaa ttggattaac catagccatg gggtatgcca ttgcgttagg gggtagcatc
    33901 aagcttcaat cctcaccagg tcaaggatca acctttactc ttgtgctacc tataacagtg
    33961 cccaactcca acgccaacaa ggaggctttc aactttactg gtaaaagaat actggtggta
    34021 gaggatgatc tgataaacta ccagtaccta cacgccttgc ttagcaaaac cggtgctact
    34081 gttattcatg ttaaaaatgg cgaagatgcc atagaggcag ccaccattga aagggcagcc
    34141 gatttaattc tacttgacat tcagttacct tttaagagcg gaatagaggc taccattgag
    34201 atacgaaaga taaataagca aatccctatc attgcccaaa ctgctaccga ttccgaatat
    34261 gtgagtgcct gtagggatgc tggttgtaac gcatttgtta caaaaccaat tgaccccgat
    34321 gaactactaa acctcattaa acgtttccta taaaaacaaa accccaactt gagttggggt
    34381 ttacgattaa tttgacagtt ctttatacgt tgaaacggat atgcagaata tcaccatcct
    34441 caacaacgta gtttttaccc tcaacaaata gttttccctt ttcgcggcaa gcgtgctctg
    34501 aaccaagggt aataaagtcg ttatacttca taacctcggc cctaataaag ccgcgttcaa
    34561 ggtcggaatg gataactcca gcagcctgtg gagctgtcat cccttttttg attgtccatg
    34621 ccctaatttc cttgggccca acggtaaaaa aggtttcgag gtttagcagg gagtatgccg
    34681 ctctaataag cttgttaacg ccgggctcag taagccctac atcggccaaa aaggctttgc
    34741 ggtcatcctc actctcaagt tccgatattt ctgcctcaac cttaccggct ataataagta
    34801 tttccgtttt ttcatccttt aggaactcct ttaccctatt ggagtactgg ttgccgttta
    34861 cagccgattt ttcgtcgaca ttgcatacgt agataaccgg ttttgtggta agcaggaaaa
    34921 gatcgtcaac atacttttta tcgtgctcat caactggaac agttgaagcg tttttgaacg
    34981 actcaaggtg gtttttgtaa accgatagaa cctcaaaagc ttttttggct tccttatcgc
    35041 ctacctttgc agctttttca accttttgta gctttttctc aaccgattcc aggtcgcgaa
    35101 cctgcagctc aaagttcacc gtttcaatat cgcgaacggg gtcaatggaa ccgtcgatat
    35161 ggggtagcga gggatcgtcg aaacagcgta gcacgtggat taatgcatcg cagttacgaa
    35221 catcggccag gaacttattg ccctcgccct taccagttgt tttcgataaa ccgggaatat
    35281 caacaatctc aacggtggtg tgaacaatac gctcagtggg ttggaacttt tcaagttcgt
    35341 aaagccggtt atcggggacg tttattgtac tgatgttcga ttttcctgtg ccggtagcca
    35401 cattgctggt taaggcttta gtgctcgaca tgcagttgaa aatagtagtt ttgcccacgt
    35461 tggcaagacc aattatgcca cattttagac ccattgctat tcaaattatt tgagcacaaa
    35521 attacagcaa ataggtcaga taacaaaaaa agcggctact gccgcttttg agtttagttg
    35581 ctttccagca gctggagtat ttcgtccatt tttggggtaa gaataatctc agtgcgccta
    35641 tttttttgtc gagcttcggg tgttttagcc ctatcgatgg gaaggtactg gctccgccct
    35701 gctgcagtta cacgcacagg gtcaatcctt gagcctgaaa tcagtaggcg tacaatggta
    35761 gtggcacgtt ttacgcttaa atcccagtta tcctggagcc ctcctgttcc tcggtatgga
    35821 acatcgtcgg tgtgaccctc aaccattatg ttaatatcgg ggttctgttc aagaaccggg
    35881 attaactgct ttattgcagc ggcacccttt gagtcaatct cgtagctacc cgatttgaac
    35941 atcagctttt cctccagcga tacgtaaacc ttgccattct gaattgagac tgtgagcccc
    36001 ttgccctcga acccaaaaag cgcatcggca acttttttcc gcagggctct cataactgaa
    36061 tccttggcat ttagcattcg ctccaactca accaaccggg cattgcgtgc atcaagctca
    36121 cgtttcatgc tatcgaggtt tgcctgcacc ttatcgagcc caagcttacg ctgatatagc
    36181 ctccgctcaa gttctttaag ggcatcttcc ttttcctgca actcttcctg ggttttctgt
    36241 atttgaagta aaagcttttt ggtttcatcc ttgctacccg aaattacccc atcctgtagg
    36301 ctttgaagct ctgcgtaacg ctccttaagc gttgttagct cctcattaat gcggttgaaa
    36361 gtaactgcac ggtttatgct gtcgcttgtg agggcatcaa cctttgcctg aagaatacgt
    36421 gttttactgt cgagttccga gtttttagtt gtaagggcct cgttttccac cgttagcttt
    36481 tcacgctcct cctcgcactt tgcgtttttg tcctgtagcg cttggaattc cttggcaggc
    36541 acgcatgcag ttagaattgc taacccaagt atcagggctt taaagtattt tggacattgc
    36601 ttcatgttgc aattattttg aacaaatata aaaatttaat tgcaccctcg cattttgaaa
    36661 cattcttaaa atatcaacat aaaactacta tttagacaga aaagatttgg aaaaatatgt
    36721 aacaaagtgc tattttagct gttaaggtat agtgagggga atggtgttta tggagaacaa
    36781 gtacgcattg ctaagcaata tcgattcgcc cgaggatatt aaaaggttaa aacctgagca
    36841 gctggttgat ttaagcgctg agctcaggca gtatatcatt gatattgttt cggagaatcc
    36901 gggccatttt gcatcgagtt taggggtggt tgagctaacc attgccctcc actacgttta
    36961 taacactcct tttgaccgta taatttggga tgttggccat caggcctatg gccacaaaat
    37021 acttactggc cgtcgcgatg ttttccacac aaaccgtaaa tacaagggaa ttagcggatt
    37081 ccctaagcgt tccgagagtc cgtacgatgc ctttggcgtt ggccattcat ccacatccat
    37141 ttccgcagcc cttggcatgt cggtagcatc gaggttgaag cttgaaaacc gaaaagtagt
    37201 ggctgtaatt ggcgatggtg cgcttactgg cggaatggcc tttgaagccc ttaaccacgg
    37261 gggtggtatc aacccaaaca tgctagttat ccttaacgac aacaatatct caatagatcc
    37321 tagtgttggg gctctgaaag agtacctgct cgatatcacc acatcaaaaa cctacaacaa
    37381 gatcaaggat gaggtgtggg atattcttgg acgaatagat agggttggtc ctaagacccg
    37441 tagcctaata cagaaattag aaaacgccat caaagcagcg atactcaagc aaagcaatct
    37501 gtttgaatcc atgggcttta gatattttgg cccaattgat ggccacgacg tggtttacct
    37561 tacacgtgtt ctccagg
//
LOCUS       DAOSAL010000018        35124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_888_length_35124_cov_45.700448, whole genome shotgun sequence.
ACCESSION   DAOSAL010000018 DAOSAL010000000
VERSION     DAOSAL010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 35124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35124
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_888_length_35124_cov_45.700448"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="PK049_04245"
     CDS             complement(<1..514)
                     /locus_tag="PK049_04245"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOK60802.1"
                     /translation="MNRLKQFLIILVALLLGNLSLMAQEISVTGKVTDAADGTPMIGV
                     AIVVKGTTTGTITNVDGEFSIKCKGNDVLVFSFVGYNTQEVAVGSRSVINVQMKQESK
                     EIDEVIVIGYGVQKKSDRTGSVVNVKAEELNRGSLTDPIQAMQGKVAGVNVSKKGGDP
                     NSGFAVQIRGA"
     gene            801..1817
                     /locus_tag="PK049_04250"
     CDS             801..1817
                     /locus_tag="PK049_04250"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOK60803.1"
                     /translation="MKSSQVTIKDIARELGISPSTVSRALKDHPDISQETKRLVNELA
                     AKYNYKPNIIALSLRNQRSNVIGVIIPEIVHYFFSSVISGIEEVANEHGYSVMISQSV
                     EDYNREVAACDTFLNGIIDGILVSVSKETANYDHFHRFEEEGIPIVFFDRAVDEIQAD
                     KVIIDDFDGAYQATEHLIVQGRRKIVHFAGPQNRLIGQNRLNGYLKAMRDNGVVIDER
                     LIIPCDTFQSALVETQKLIDSGLKFDSIFTVNDFTAAGAMKVLLRNGYKIPTDIAVVG
                     FGDDQTSLMVEPTLTTVNQPGFEMGKKAMEQLIRRITQSKPEPPVTEILKTQLIVRES
                     SRVK"
     gene            1945..4380
                     /locus_tag="PK049_04255"
     CDS             1945..4380
                     /locus_tag="PK049_04255"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HOK60804.1"
                     /translation="MKRWILLFSLSFLVGNIALGGRNKDSLRITREIYAIRATEHVYI
                     NGRLDENVWGKSDFANNFTIYEPYNGQKASQQTEVSVLYDDFAIYIGARLFDTSPDSI
                     FRELGQRDNSDRLRSDAFSVYLSTYNDGVNYLQFTVSASGVQTDIKITSNQSDRNWNA
                     VWESAVGFDSLGWIVEMKIPYSALRFSKNVNQVWGINFSRLIKRKNEVSTWNFVDKAQ
                     AGFITQSGILKGISGVKPPLRLSFLPYISGYVDQYAGTKGLDSRVSGGMDLKLGLAQS
                     FTLDVTLIPDFGQVRSDDKVLNLTPFEVKYDEARQFFTEGTELFGKAGIFYSRRIGGT
                     PAFYDAAYDSLKTNEIVDKNPLETKMVNASKLSGRFANGLGFGFFNAMTENTYAEILD
                     TVSGKRRKLLTQGFTNYNITVLDQNLPNNSYVSLINTNVYRPVDSFIANVTATEFNLK
                     NKKQSFGISGVGALSQRFVDSVQHGFKTNLSISKISGNIQGYIWANIESEHYNPNDLG
                     YLRSPNELSSGVALAYKVFEPFWRLLNFEAQLEFSNSRLYTPKAFQSQEIEFQAKFTN
                     TNNFTGGFNASYNPRYGYDYYEPRVEGHKFLTPHVLWLQWWGSPDYRKTVALDHRFSF
                     WYADMYNQKGYMYSISPRIRFSDKLLTIYSWAYQSERNNIGYYDYDGSNIFFGSRKMV
                     TITNTLNIQLAFTAKSFLSIRARHYIKHFKYNEFFTLKNDGLLEQTNSITNPANRNYN
                     LFNIDMFYQWHFAPGSELVFAWKNQAEELLDNPQYLYFKNIEGILNSPQNNSFSIKIL
                     YYIDYQMIRKFKS"
     gene            4429..5469
                     /locus_tag="PK049_04260"
     CDS             4429..5469
                     /locus_tag="PK049_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HOK60805.1"
                     /translation="MFSRGFASDNNSGVHPRILEAINDVNHGHTIAYGDDPITQKAID
                     RFKQIFGNDIEVFFVFIGSAANVLGLKAVTQPHNAIICAETAHINVDECGAPERFTGC
                     KLLSVPTVNGKLTIDGIKKHMHGFGFQHHSQPKVISISQVSELGTVYTVDEIKTLADF
                     AHSHNMLLHMDGARLANAAVSLGRGFREFTRDSGVDVLSFGGTKNGMMYGEAIVFFNQ
                     QLAHDFMYTRKQGFQLASKMRYISAQFLAYLEDGQWDSTARHANAMAQLLAKKVSAIQ
                     GVTITQPVQANAVFAIVPPELIHPLQSEYFFYVWDEDRSEVRWMTSWDTTEQDIDRFC
                     QLLEELMRTVKR"
     gene            5695..6996
                     /locus_tag="PK049_04265"
     CDS             5695..6996
                     /locus_tag="PK049_04265"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOK60806.1"
                     /translation="MIILLSAILIGFLAFSFGIYLKKNNRTLALHIAESSKYAKVDLL
                     SVNNALTNSNEYSSINRQFEQFVNKWDLVGATFCIAHKGKIIYARGFGMANKEEGLAM
                     QPYNTMRVASVSKLITATAVMKLVEEGKLRLNDRVFGADGILNDEKFLNYKDKRVEEI
                     TVHNLLNHSGGWTPRWGDHLFIKDVIARELHKDLPLDVDDYIVFALSKRLHFQPGSHS
                     SYSNIGFVILQRVIEKASGMDYESYVKNEIFKPLNIYDAHLANNWDSLRYDNEVRYYE
                     VPEAEKVISFDGSKQSVLKSRGGNDVRALGAAGGWVISPISLTRFVMAVDGRNDFPDI
                     LSKQSVDIMKKTYNGKFQPLGWRWVRDDGTLWRSGSMAGTSALVISRPDGYTLTFTSN
                     QSHWKGARFPYVVDRFFLRIFNKYIKSIPDHNLLNVDKGKY"
     gene            complement(6993..7595)
                     /locus_tag="PK049_04270"
     CDS             complement(6993..7595)
                     /locus_tag="PK049_04270"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HOK60807.1"
                     /translation="MGSTTKYMAMFPLPVFLLPGEEIPLRIFEPRYKQLINECNETGD
                     TFGIPYVSNNNIKSYGSEVELVNIVAKNSLGEMVILIKAIANFHLLNLKEKLPDKLYG
                     GGIIEYIDDRFTTTNPELVVLVKKLNLNLDPIMGSLITGDSVNLMNVAKAIQLDSDEK
                     FAFYSLRNEKQMVNYLIKRLRFIEHIQLQEKLLENNFSLN"
     gene            complement(7599..7985)
                     /gene="msrB"
                     /locus_tag="PK049_04275"
     CDS             complement(7599..7985)
                     /gene="msrB"
                     /locus_tag="PK049_04275"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HOK60808.1"
                     /translation="MKSDDLRNRLTDIQFYVTQQKGTERPFTGEYWNHFEPGLYVCVC
                     CGAELFQSDTKFHSSCGWPSFFDSKFVENIKFQKDLSHGMVRTEVLCKNCGAHLGHVF
                     NDGPEPTGIRYCINSASLKFIPNKKE"
     gene            8186..8863
                     /locus_tag="PK049_04280"
     CDS             8186..8863
                     /locus_tag="PK049_04280"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HOK60809.1"
                     /translation="MKKIILALSVSLVSAIGYAQQTAVYSLKEFDKLRVWGEFKVFLV
                     KGDSLNAKVETQSIPASDIALDVNGNTLEVKLKGKLYDRVSATVYITYRELREISVSA
                     AAAVSLQDTLKATNISININTSSEFDGAVLAETADLAVGQGSTLRIRGSVSSYEAKVN
                     TGGVLTAIDLIAPKTYVKVNTGASAKVYASKVLEANVSTGGSLTYTGSPEQKKIKTLV
                     GATIIEQ"
     gene            complement(8866..9819)
                     /locus_tag="PK049_04285"
     CDS             complement(8866..9819)
                     /locus_tag="PK049_04285"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HOK60810.1"
                     /translation="MIWQFISSKIEQNIDVYLITVAETQGSSPGKVGFKMAVAADGSL
                     YGSIGGGVMEFNLVEMARKNLAEGYKQNFLKRQVHSHDAAEDKSGMICSGEQTQIFSL
                     FSKADSEVLAKVIELESENRLGKIVLSTSGAEVYEGESHISIKGIEFQKSDSSFRYSE
                     PLGVKETVYIFGAGHISVPLSQILGLLDFRVELFDNRQGLSTFEGNVWAHKKQIVDYK
                     QVSHLLPGNDRSYVVIMTFGHKFDEIVLKQLIGKPLKYIGMIGSKSKVKTIFESLIAE
                     GYGKENVERVCSPIGLPIGGQTPAEIAVSIAAQIVEWRNKR"
     gene            10039..11226
                     /locus_tag="PK049_04290"
     CDS             10039..11226
                     /locus_tag="PK049_04290"
                     /inference="COORDINATES: protein motif:HMM:NF009555.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HOK60811.1"
                     /translation="MSDVYSKIKELSEKYADYTARNLSRLVQIKSLSAQERDVQLELK
                     RQMEEAGFDEVFIDGLGNVIGRIGNGKRILAIDGHMDTVDIGNKDNWEFDPLGGEIRD
                     GYVHGRGTVDQEGGPAAFVTAGKILKELGFNRDLTIYFVGSVMEEDCDGLCWKYIVEE
                     DKIRPDFVISTEPTNLNIYRGHRGRMEMQVHFYGVSAHGSAPERGKNAIYMASKVALE
                     VEKLNERLKFDEFLGKGSITVTEFTSSSPSLCAVSDYARIHLDRRLTWGETKESAVAE
                     IEELIKGMNAKVEVLNYSEKAYTGLEYGMEKYYPTWKMPEDSEIVQKGVSTYQKLFGN
                     APKVDKWTFSTNGIMTCGTYKIPTIGFGPGNEVLAHAPNEKVPISDLVAATAFYAAFA
                     YEI"
     gene            11506..12693
                     /gene="ygeW"
                     /locus_tag="PK049_04295"
     CDS             11506..12693
                     /gene="ygeW"
                     /locus_tag="PK049_04295"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HOK60812.1"
                     /translation="MDLIKKIAELNHYKSDLSGKDFLLTWEKSDDDLKVVLQVASILK
                     EMRKENISPRIFDSGLAISNFRDKSTRTRFSFASAANLLGLAVQELDEEKSQISHGET
                     VRETANMISFLSDFIGIRDDMFLGEGNKYMRQVGEALDEGFTKGVLPVRPGIVNLQCD
                     MDHPTQSMADLLHLANEYGSLDALRGKKIVMSWAYSPSYGKPLSVPQGIIALMTRFGM
                     NVELAYPEGYDLIPEIVEIARKNATQSGGSFKVSHSMTDAMRDADIVYPKSWAPFHIM
                     QQRTQLLMNGDKNGLKELEQVCLANNAKYKDWEYNEQVRKVTRNSDALYMHCLPADIS
                     GESCIQGEVSKETFERYRIKTYQEAGFKPYIIAAMMLTNRFQNPADILMKLIERNTPR
                     VIK"
     gene            12949..13491
                     /locus_tag="PK049_04300"
     CDS             12949..13491
                     /locus_tag="PK049_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60813.1"
                     /translation="MKELIEEIFIDAWAINDIISCYQDKKADYPDLFQIVSGYDLSKP
                     TNKIPFKVYVELCSWIEKKLGQFNLIKIGRRIGESTYSLMLETNMVNTESKPLDVMKA
                     LVLLAQKGVKDPKRRGWEIVSHTDKSIQMRKTQLFNTHIQVGLLDTLVRKCKVYGVQV
                     NLVKEESKGAEFDEYLITWL"
     gene            complement(13502..14932)
                     /gene="hydA"
                     /locus_tag="PK049_04305"
     CDS             complement(13502..14932)
                     /gene="hydA"
                     /locus_tag="PK049_04305"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HOK60814.1"
                     /translation="MRILITKGRIVTSVGEQIADLLVVDGKIAAIGNQLEANAETTIN
                     AQGMLVLPGGIDPHVHLHLPTPAGFSSDDFHSGSIAALMGGTTTIIDFVTPSRGQSMV
                     EALKARLDEAACSLVDYTFHVSPIEWNKNTPKEIEECIEMGFPSFKVYMAYKSSVGID
                     DNALYHVMHTVAQNGGMVTAHCEMGDDIDTLRDFYASQGLTAPKYHMLSRPPRFEALA
                     VKRAIDLADQTQCPLYIVHVSAAESVKHIIQAQKSGQSLYAETCPQYLLLDEALYDST
                     LPEAIKYVISPPLRQSIDRDTLWNAINNGNILTVGTDHCPFSLEQKMVGKDDFRKIPN
                     GAGGIEHRLALLYTFGALTGKLSLSKLVDVFATQPAKIFGLYPQKGELAIGADADIVI
                     WNPNVKGSISSSKHHSKSDISIYEGFETTGEVAFVIKNGIIAVDNGKLSENLPSGQLL
                     KRRSTKTTGHYYKRMDNSGSYNCGRV"
     gene            complement(14942..15565)
                     /locus_tag="PK049_04310"
     CDS             complement(14942..15565)
                     /locus_tag="PK049_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60815.1"
                     /translation="MNQQVLEWIGYSASVIIALSMTMSSIVKFRWINLTGALMFSIYG
                     FLIGAWPVGILNGFIVLTDAYYLFQIYSKKEVFEILEVRPENRYLMRFLDFHQKDIHR
                     FFPGFVYTPTENTVSFLILRNMAVAGVFIAQRNETKALEVELDYVLPEYRDFKNGKFV
                     YYWLSQKFTESGYTRVWAKACNKSHKKYLQHLGFTESGGNHFLKILE"
     assembly_gap    15868..15967
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(16256..17521)
                     /locus_tag="PK049_04315"
     CDS             complement(16256..17521)
                     /locus_tag="PK049_04315"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOK60816.1"
                     /translation="MILLSDATYIDYQTLEIKNCDILVTNGNIEFHPPKTYRGNATET
                     INCSGKLVTHSFIVGHHHAYSALARGMGAPKKSPENFYEILKYIWWTLDKCLTTEMVE
                     YSGLVTAMACAKAGATFVIDHHASPYAVKGSLETLAKAFERVGLSHLLCYEISDRDGD
                     EIAQQGLEETDSYLKNYQGLVGLHASFTLSDKTLQAAADLTQKYNSGVHIHVAEDKYD
                     QQHCHETYGKTVVERLSDFNFLNSSKSILVHCLHLSEKERNLIGQSPCWVAQNTDSNL
                     NNNVGYFSSKNLGENIMLGTDGMHSDMLQSMKSAYFVGQNFDPMSPAQAYKRLRNAHH
                     YIAQNGFSGDGPDNLVVLDYDSPTPVNAENIPGHFIYGINQSHIEHVISNGKLIVKSR
                     AITTVDQEIILKESQKLALELWKRMRGEG"
     gene            complement(17547..20105)
                     /gene="xdh"
                     /locus_tag="PK049_04320"
     CDS             complement(17547..20105)
                     /gene="xdh"
                     /locus_tag="PK049_04320"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0046415 - urate metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HOK60817.1"
                     /translation="MIRFNLNGKTVEYNGDINRSLLEYLRNDLHLTAAKDGCSGQGVC
                     GACSVEVNGSIKMACRIKMSDLSEAIIYTLEGLPNNFREILAHHFATKGAVQCGFCSP
                     GMIMRARGLWLSNPKATRSDIIRAIAPNLCRCTGYVKIIDAIQHAFAELNGQALPKNN
                     TGARIGERYPKYQAKETALGQRPFVDDMFVDGMLYGALKFSDHPRAKVLKIDTSKALS
                     VQGVVKVITATDIPGERYIGLIFQDWPVMVALGEVTRYIGDVIAGVVATSEDIARHAA
                     SLIEVEYEVLEPVTDIHQAIKPDSPQVHPNRSNVLETCTIRFGEVDKAFAEAAYVSSD
                     IYETQRIEHAFLETETALALPNGDGIKVFSQGQGVYVDRKQIAKILNLPESKVNVVQV
                     QNGGGFGGKEDLTVQGHAALMSYITQMPVKVHLTRDESIRMHPKRHPVWMKMSLACNS
                     EGKFTALKLEAIGDTGAYASVGTKVMERVVGHATGGYTVPAVDISAKTVYTNNIPCGA
                     MRGFGVPQVTFAIESCIDDLCRQGGFDRWQIRYDNALEESAKTATGQELHGVGLKKTL
                     IAVKEQFKSAKYAGIACGLKNTGVGNGMVDDSEVIIKVISPTRVEIHHGWTEMGQGVH
                     TMAIQTLHHETGIEPSCIEVIVDTDAEIPTGMTTSSRATALVANALINAARPLREDLK
                     TAKLEKLVGRVYRGKFVCDWTCKPGAKTDKPVIHFAYGYATQVAILNEQGKLSKIIAA
                     HDAGKIMNQMLFEGQIEGAVHMGMGYALTEDLPMENGFPVSFKFKDIGIIRANQMPEI
                     EVIGIEEPDPIGPFGAKGIGEIGMVPTAAAIANALWTFDGIKRTRLPMMRNKSE"
     gene            complement(20213..21448)
                     /gene="thrC"
                     /locus_tag="PK049_04325"
     CDS             complement(20213..21448)
                     /gene="thrC"
                     /locus_tag="PK049_04325"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOK60818.1"
                     /translation="MNGKFILTCSGCGKTFAPSPELYLCPDCSRNNDTVNPPKGVLKA
                     LYNYEILSKHKEEVITGNFWDLIPLLKKESLPKLRVGETPLYRFDRIDGETLTFELYL
                     KDDSQNPTFSFKDRASAVVSAYAKENGISTIVAASTGNAGSSLAGICASQEQKAIIMV
                     PKSAPHAKLTQIVMYGATIVPVNGTYDMAFDLSMEATKEFGWYNRNTAFNPLTIEGKK
                     TVSFELFKQLSRAIPDYIFVPVGDGVIISGVYKGYEDLLKIGLIDRMPTVVAVQAAGS
                     SNLIENIGKPDFTIKPSNTLADSISVDIPRNFFMAQDYLLQYKGEWLSVTDDDIVNAS
                     KILARNTGIFAEPAASTAFAGMLCYQKNQKLLTKQRVVVLLTGSGLKDLKAVSSVINI
                     PNAIDPTIDSLRKNEWIKF"
     gene            complement(21460..>22599)
                     /locus_tag="PK049_04330"
     CDS             complement(21460..>22599)
                     /locus_tag="PK049_04330"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOK60819.1"
                     /translation="AYGCLAPRIITGGFDPTTQKAVWPSTGNYCRGGAFDSKLMGTES
                     VAILPEEMSRERFTWLRDYVGSEVIATPGCESNVKEIYDKCWEIRRTKPDYVIFNQFD
                     EFGNAAWHYNITGKAIEEVFNELGGKSNLAAYVSATGSAGTIAAGDYLRTIAPHIKVV
                     ASEAKQCPTILMNGFGGHRIEGIGDKHVPWIHNVKNTDVITAIDDEDCMRIFRLFNEP
                     EGVKYLVESGVPVEIANNLKLLGISGISNMLSAIKTAKFFEMTSEDVIFTIATDSAEM
                     YGSRLEELREERGSYSQIQAIKDFEKCILGATTDWMRELTYHDRKAIHNLKYFTWVEQ
                     QGKEIDDLNRLWNDRELWPKLFSQVKRWDELIDEFNDRVGLLKSL"
     assembly_gap    22600..22699
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <22700..23162
                     /locus_tag="PK049_04335"
     CDS             <22700..23162
                     /locus_tag="PK049_04335"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOK60820.1"
                     /translation="MRNIDEIDRKLLNILQDNSRVTIRELSERLHLSTTPIHERIKKL
                     EKNGYIKRYITLLDPKALGKKLTVFISVSLSSHTKEVVDAFEHEVKKLPEVMECYYVS
                     GNWDFLLKVQCNDMEDYHNFVIHKFSVIKNITQFYSSFVMSESKQTYKYEL"
     gene            complement(23214..24296)
                     /locus_tag="PK049_04340"
     CDS             complement(23214..24296)
                     /locus_tag="PK049_04340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOK60821.1"
                     /translation="MRILQLVTRFDFGGAENHVRELCNELAASNHQVILLSRKGRQNE
                     LLDKRVKFIQLPSFVSYLILTKVVIIVYLLLRHKIDVIHAHQRLPIISACIGGFILRV
                     PVVATVHGRVRHDLRSIVAKKLTSKIIFVRNQAISNLDRFRSIQNKSVYIPNGIPIPK
                     KLPLIETNTIGYICRIDKRHFRVIKLLILACEKLKLSQPTIKLLLIGDGNNVEELKEL
                     INKTNLKFESTAIEYLGFVRNIDLLNSFPELILGVGRVAIEGLIRGATVISIKNNKMG
                     RIITPSNYDDYAFTNFIKIDGCVPTEESIYNELKLYFERRNEYRKNTSILAARIENDF
                     GISKTTNRIIEVYSSTLKSRGLPNSK"
     gene            complement(24460..>26400)
                     /locus_tag="PK049_04345"
     CDS             complement(24460..>26400)
                     /locus_tag="PK049_04345"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOK60822.1"
                     /translation="KTKRKLDYFDCIAAPCVNTCATNQEIPTYLHFAATGNYPKALEV
                     ILRTNPFPNTTGMVCDHLCQNKCTRINYDNPLQIRDVKRYVAENADIKLSPAQPNGKR
                     VAIIGAGPSGLSCAFYLAINGFKVDVFESKTKAGGMVRYAIPGFRLTDQAIDRDIERI
                     TQLGVNIHYSHPVTRSEFSDLKNKYDYVYISAGAQKSAILDIEGIDADGVIDPLTFLM
                     GVKSGNIKQIGKRIVIIGGGNTAMDAARTAHRLVGDNGKVTIVYRRTVNEMPADEGEI
                     KAVIAEGVELVELAAPEKIITQNGKVKALKCRLMKLDGYDSKGRPKPVKVEGSEFEVE
                     CDVVIPAIGQALDIDFVPNELLKADTRNYKTLMGNVYIGGDALRGASTAINAIGDGRK
                     AAQEIISQCGFTGISTSVPEKKHNYRDLMLLRSTRKYGYVHKELTAEKSKTFELVTET
                     PDETTIKEESARCLHCDEVCSICTTVCPNLANFAYTIEPVRYQLDKVLVGSDGTQEVQ
                     PDHFFEVKQTVQILNIANFCNECGNCSTFCPTEGAPYQDKPKFHLTTKSFKESEDGYM
                     LSVLKDRNVLIFKQKGGIKTLSENRDEYIYETDHVYARFSKNGFKLVEAKALTPCVRQ
                     IQFQHAVEMSVILQGAKRLMGV"
     assembly_gap    26401..26500
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<26501..27561)
                     /locus_tag="PK049_04350"
     CDS             complement(<26501..27561)
                     /locus_tag="PK049_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60823.1"
                     /translation="MSDKFSPIPAKQLLEIILKELDTHGTVFGYPSELFFNPNQGKLP
                     TTIFGQTIDTPIGVAAGPHTQLAHNIVIAWLMGARYLELKTIQTLDELEIAKPCIDMQ
                     DEGYNCEWSQELRVEESFMEYLKAWVIIHILNHKLQLGSKPNMVFNMSVGYNLEGIKK
                     PNVQWFLNKMGNATDELNQMVQELKAIYPAIGNIDIHAKLSDNITLSTMHGCPANEIE
                     DIARYLLTERRLHTLVKLNPTLLGSSELRETLNNKLRFKTNVPDIAFEHDLKYPDALK
                     LIEALSAEAKTLNLTFGVKLTNTLESVNNKHVFPGEVDMMYMSGRALHPISINLARKL
                     QNDFKGQLLLSFSAGVDAF"
     gene            27663..28271
                     /locus_tag="PK049_04355"
     CDS             27663..28271
                     /locus_tag="PK049_04355"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOK60824.1"
                     /translation="MKENFSAIILAAGSSGRMGQPKALLPFDPQRCFARKLADEFLAF
                     GCQRVVMVTNAQVAAQAVSAAYKLNGIEIVINPNPELGRFYSFYLALQAIGNHYVFMT
                     NADNPFVGADILRALWRHAMQADYVYPVYEGRGGHPILISPRIVRAARAETNPDINLK
                     EYLNRFSKACIPVPNDQILININTPEEYTRLIADGKLPGNEI"
     gene            complement(28576..29028)
                     /locus_tag="PK049_04360"
     CDS             complement(28576..29028)
                     /locus_tag="PK049_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60825.1"
                     /translation="MQDKNVFDPEKIALIDFKMIKGQVNTPENFDINKVVGYHLDNSL
                     QLSFNLDDKLAKADFTVNVKTDSKGENESEATGNFHLIFIYRIENLEELTILKKNKRL
                     NLNPGLANALSSVTYSTSRGILITRLQGTALQNFVLPIINPNKLLHNK"
     gene            complement(29018..29431)
                     /locus_tag="PK049_04365"
     CDS             complement(29018..29431)
                     /locus_tag="PK049_04365"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOK60826.1"
                     /translation="MRSKTVDRLLKSTPKDVEIFVDWYADLVVRINQLLREKNISKKE
                     LSVKMNKKPSEISKWLNGEHNFTLRSLAKLSAELGVPLIEVTKKKVQTEFVEDGFVCR
                     VHTFVSYTKLNTKTTENVWQCAEETEPYNELSNAG"
     gene            complement(29436..29972)
                     /locus_tag="PK049_04370"
     CDS             complement(29436..29972)
                     /locus_tag="PK049_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60827.1"
                     /translation="MNTFAIEIWYDEGSICTFYTVRWVTDDDNAPSETDRFFDTYAVP
                     EHPLNEKAMQLFRLITESIGNRYGATNDFFDRIVNKAQELPPKQKQWVEEIKDLGINF
                     PLRLFCYRVTENIVILFNGGIKESQASQESKNLRMKFYEAQTFVKKIEEALQSQMIKI
                     SDNGRYLQNFDGTTDIIL"
     gene            complement(30353..30907)
                     /locus_tag="PK049_04375"
     CDS             complement(30353..30907)
                     /locus_tag="PK049_04375"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOK60828.1"
                     /translation="MEVLFDACVFFLLFYPTFLRAQAAVETYKQAIRLKPNYVEAYYK
                     LGMDYVNLGRYTEAVEAYKQAIRLKPDYADAHFNLGLTYGQLGRYTEAVEAYKQAIRI
                     EPDDAEAHNNLGTAYDKLGRYTEAVEAFKQAIRLKPDLAMVHYNLGVAYLHVGDKSSA
                     FEVYKILKELDKDLSNKLFNLIYK"
     gene            complement(31212..33467)
                     /locus_tag="PK049_04380"
     CDS             complement(31212..33467)
                     /locus_tag="PK049_04380"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOK60829.1"
                     /translation="MKNIDTIGHVTGKSVYLDDIPTISGTLHAAVVGSAIAHGKILKV
                     DTTEALRLDGVVKVITCTDIPGENQIGGIVEDETLLAEGEVHYQGQPIAVVIAKTERD
                     AHKAAKMVRIDYQELEPVTDPRIAKEKGLLLIPPRTFSMGNPNEVWSQCKYIVEDSIE
                     LGGQEHLYIETQGAYAYPVEGGYIKVHSSTQGPTAVQKTVARVLGLPMNLIEVDVVRL
                     GGGFGGKEDQASGFAAMAALAAHITGKPVKLVLPRHDDMRMTGKRHPYSGDFRIGLTA
                     DLKILAFETTLYQNGGAAADLSPAIMERTLFHSTGSYYIPNTRVTVYSCKTNLPPNTA
                     FRGFGGPQGMFVIESAIAKAAETIGVSAIEIQQANLLKENDEFQYGQKAENVNARKCF
                     ALASERFDLENLRKSIDNYNQNNKYSKRGLAVMPLCFGISFTNTAMNQARALVHIYQD
                     GSVSVSTGAIEMGQGVNTKMAQVASILLGIDISRVKIHSTNTTRVANTSPTAASSGAD
                     LNGNALRIAIAALKKRLLKVAAELLECSDDELNIENETVIRNGAPTALKWNELVKKAH
                     LKRVALTENGHYATPVIYFDKTKEKGHPFAYHVYGTGVVTVTLDCIRGTYTIDDVYIV
                     HDFGNSINPLIDLGQVEGAVVQGIGWLTLEELAFDSKGRLLSNSLSTYKVPDIHFAPK
                     SIQCIPLPTNGPELAVLRSKAIGEPPFMYGIAAYFAIHNAIKAFNPHYSIKLTAPLTP
                     ERVLMNLYQGR"
     gene            complement(33479..34876)
                     /locus_tag="PK049_04385"
     CDS             complement(33479..34876)
                     /locus_tag="PK049_04385"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HOK60830.1"
                     /translation="MISFILNDKPVNTSCNPGMSLLDFIRYESNLPGTKIGCREGDCG
                     ACTVLIGELTNDTVEYRSVTSCLYPLINAHGKHVVTIEGINLSNTLSPVQQAMADNAA
                     TQCGFCTPGFVMSFTAFCMNHETPTTERAIEAVAGNICRCTGYKSIERAAADIARVMQ
                     EKKISNPVGWLVGKGYLPNYFLEVPGKLKQIKAFDDESSSTSQLIAGGTDLMVQRPES
                     IYEQKVKSTLDGHLPKEITLNDCTCTVGGATRVSDLVASAELMERFPHLKEYLMLVSS
                     EPIRNMATIAGNIVNASPIGDLSVMLLALNAQITSVSDGKTRQQPLNQLFKGYKQLNL
                     NAGEVIQYITFELPKSTTQFSFEKVSKRKYLDIASVNSAMLIEVENGKVKKCRISAGG
                     VAPIPLYLHQTSNYLSGKEINSQSVTQAITIMHDEISPISDIRGTAEYKKLLLKQLFL
                     AHLQRFLGKQFDQIF"
BASE COUNT         9495 a         7443 c         7375 g        10511 t
ORIGIN      
        1 cagcgcctcg gatttgaaca gcaaagccgg agttggggtc accccctttt ttcgacacgt
       61 ttacgcctgc taccttaccc tgcattgcct gaatggggtc ggtaagcgaa cctctattta
      121 actcttcagc tttaacattt actacagaac ctgtacggtc gcttttcttt tgaacgccat
      181 acccaataac aataacctca tcaatttcct ttgattcctg cttcatttgc acgtttatta
      241 cacttctgct gcctacagca acttcctgcg tattataacc tacaaatgaa aaaacaagaa
      301 catcgttacc tttacattta atggagaatt ctccatcaac atttgtaatt gtacctgtgg
      361 ttgttccttt aacaacaata gctactccaa tcataggtgt accatcagca gcatcagtta
      421 ctttcccagt aacggaaatt tcttgtgcca ttaaggataa gttccctaat agcaatgcta
      481 ctaaaataat tagaaactgt ttaagtctat tcatggcaaa tattttattt tgagttggtt
      541 tttaatttgt taaacattaa aactactaca tcaaaacttt cacaattaac aaaattttaa
      601 cactggaaac aaggaaaaac attgatttta ggcctgtttt ttatgcaaac gataccgcaa
      661 acgtttgcat tttgataaaa aaatattaca ttgggcgagt ggcttttatt gcttgttttt
      721 agtagtatat ttgttaggaa tgtaacaata gcaataagta aataacaacc tatttatcaa
      781 ttagttcaaa tgctttgaaa atgaaatcga gccaggttac catcaaggac attgcccgcg
      841 agttgggaat tagcccatca accgtttcgc gtgccctaaa ggaccaccct gacatcagcc
      901 aggaaaccaa gcggttggtt aacgagcttg ccgcaaagta caactacaaa cccaacatta
      961 ttgccctcag ccttcgcaat cagcgtagca acgtaattgg ggttattatt cctgaaattg
     1021 tgcactactt tttttcatcg gttatcagtg gtattgaaga ggtggctaac gaacatggtt
     1081 acagcgtgat gatcagtcaa tcagttgagg actataaccg cgaggtagct gcatgcgata
     1141 ctttcctaaa tggcattatt gatggtattt tagtttcggt atcaaaggaa acggctaact
     1201 acgaccactt ccataggttt gaggaggagg gtatccccat tgtctttttc gacagggctg
     1261 ttgatgaaat tcaggccgat aaggtaatta ttgatgattt tgatggcgct taccaggcta
     1321 ctgaacacct cattgttcag ggccgtcgaa aaattgttca ttttgcagga ccgcaaaaca
     1381 ggttaattgg tcaaaaccgc cttaacggtt atttaaaggc catgcgcgac aatggggtag
     1441 ttattgacga gcgactcatt attccatgcg acacctttca atcggccctt gttgaaaccc
     1501 aaaagcttat tgatagcggg ttaaagttcg actcaatttt cactgtaaat gactttactg
     1561 ctgcaggagc catgaaggtg ttactgcgga acgggtataa gattccaaca gatattgctg
     1621 ttgttggatt tggcgacgac caaacttcac taatggttga gcccactctt accaccgtta
     1681 accaacccgg atttgagatg ggtaaaaagg ccatggagca acttatccgt aggattaccc
     1741 agtcaaaacc tgaacctcct gtcactgaaa tactcaaaac tcaacttatt gtaagggaat
     1801 catccagagt aaagtaaaac ccatttacaa aataatactc ctaatagcaa taccccatta
     1861 tggggtattt tttttgtaat gacttaaacc atttttacct tttatactct tacatgtaag
     1921 aaacaaactg ggattttttt acgaatgaaa aggtggattt tgcttttttc cctttctttt
     1981 ttagtgggaa atattgcttt aggaggtcgt aataaggact cgttaaggat aacacgtgaa
     2041 atttatgcca ttagggctac tgaacacgtt tatattaatg gtaggctcga tgaaaatgtt
     2101 tggggcaaat ccgattttgc caacaatttt accatttatg agccatacaa tggccaaaag
     2161 gcatcgcaac agacagaggt tagtgtacta tacgacgatt ttgccatata cattggagcc
     2221 cgacttttcg atacctcccc cgatagcatt ttcagggaac ttggacagcg cgataacagc
     2281 gataggttgc gatccgatgc tttcagtgtt tacctgagta catataacga tggagtaaac
     2341 tatttacagt tcaccgtttc ggcttcgggc gttcaaaccg atattaaaat tacctcaaac
     2401 caaagcgata gaaactggaa tgcggtttgg gaaagtgctg taggtttcga tagcttgggc
     2461 tggatcgtgg agatgaaaat accttacagc gctttacgat tctcaaagaa tgttaatcag
     2521 gtatggggaa tcaatttttc acggttaatc aagcgtaaaa atgaggtgag cacctggaac
     2581 tttgttgaca aagctcaagc gggttttata acccagtctg gcattcttaa agggatttcg
     2641 ggagttaaac caccacttcg gttgtcgttt ttgccctata tctccggtta tgttgaccag
     2701 tatgcaggta caaagggatt ggattcaaga gtatcaggtg gcatggactt aaagttgggc
     2761 ttggcccaga gtttcaccct tgatgtaacc ctcattcctg attttggcca ggtccgttcc
     2821 gacgataagg tgctaaacct tactcctttt gaggttaagt acgatgaggc ccgtcagttc
     2881 tttactgagg gaactgaact ttttgggaag gcaggcattt tctactcccg acgtataggc
     2941 ggaacccctg ccttttacga tgcagcttac gattcgctga aaacaaacga gattgttgat
     3001 aagaatccac ttgagaccaa aatggttaat gccagtaagc tatcggggcg ttttgccaat
     3061 ggccttggtt ttggtttttt caatgccatg accgaaaata cctatgctga aatactcgat
     3121 actgtttcag gtaaaaggag aaagctgtta acgcagggtt tcaccaacta taacattact
     3181 gtactcgacc agaatttacc gaataactcg tatgtaagct taataaatac caatgtttac
     3241 cgacctgtcg atagctttat cgcaaatgtt actgctacgg agtttaatct aaaaaataaa
     3301 aaacaatcct ttggcattag tggggttggg gcactaagcc aacgttttgt cgattcggtt
     3361 cagcatggat tcaagactaa cctgtcaatt tcaaaaatta gtggaaatat tcaggggtat
     3421 atttgggcta acatcgagag tgagcattat aacccaaatg atttaggcta cctgcgtagc
     3481 cccaatgagc tctcctcggg cgtggccctt gcttataaag tttttgagcc attctggagg
     3541 cttttgaact ttgaggctca gctagagttc tcaaacagtc ggttatatac tcctaaagct
     3601 ttccaaagcc aagaaattga gtttcaggca aagtttacga acaccaacaa ctttaccgga
     3661 ggctttaatg catcgtataa tccccgttat ggttatgatt actatgaacc aagggttgag
     3721 gggcacaaat ttcttacacc tcatgtttta tggcttcagt ggtggggttc acccgattat
     3781 cgtaagactg ttgcactgga tcatagattc tcgttttggt acgctgatat gtacaatcaa
     3841 aagggttaca tgtatagcat tagccctcga ataagatttt ccgataaact cttaaccatc
     3901 tacagctggg catatcagtc ggaacgcaac aacattggat actacgatta cgatggttca
     3961 aacatatttt ttggtagcag gaaaatggtt acaataacaa atactctaaa tattcagctc
     4021 gcttttacag ccaaatcatt tttaagcatt agagctcgcc actacataaa gcacttcaag
     4081 tacaatgagt tttttacctt aaaaaatgac ggcttgctag agcaaaccaa ttcaataacc
     4141 aatccggcaa accgcaacta taaccttttc aacatcgata tgttttacca atggcacttt
     4201 gcacctggta gcgaactggt ttttgcatgg aaaaatcagg ccgaagagct gttagataat
     4261 ccccagtacc tctactttaa aaacattgaa gggattttga atagtccaca gaataatagt
     4321 ttttccatca aaattctata ctatattgat taccaaatga tccgaaaatt caaatcgtaa
     4381 tggctgctaa cccataaaaa agttaacttt accgaaaaaa tctacattat gttttcacga
     4441 ggatttgcta gcgataataa ttcgggtgta caccctagaa tacttgaagc aattaacgat
     4501 gtaaatcatg gtcacaccat tgcttatggt gatgatccaa ttacgcagaa agcaattgat
     4561 agattcaagc agatttttgg caacgatatt gaggttttct ttgtttttat tggctctgcg
     4621 gccaatgttc ttggactaaa ggctgtaaca caaccccaca atgccataat ttgtgctgaa
     4681 actgcccata tcaacgtaga tgagtgtggt gcacctgaaa ggtttaccgg gtgtaagctg
     4741 ttaagcgttc caaccgtaaa cggtaagtta accattgacg gtattaaaaa gcatatgcac
     4801 ggtttcggtt ttcaacatca ctctcaacct aaagttattt ctatatcgca ggttagtgag
     4861 cttggaactg tttataccgt tgatgagata aagaccctgg ccgattttgc tcactcccat
     4921 aacatgcttt tgcatatgga tggtgctcgt ttggcaaatg ctgctgtgtc gctcggaagg
     4981 ggtttcaggg aatttacccg cgattcaggc gttgatgttc tctcctttgg aggaaccaag
     5041 aatggcatga tgtatggcga ggccattgtc tttttcaatc aacagttagc gcatgatttc
     5101 atgtacaccc gcaagcaggg ctttcagttg gcttcaaaaa tgaggtatat atcggcccag
     5161 ttcttagcct accttgagga tggtcagtgg gactcaaccg ctcgtcatgc taatgcaatg
     5221 gctcagctgc tcgccaaaaa ggtttcggct atacagggcg ttacaatcac tcagcctgtt
     5281 caggctaacg ccgtttttgc aatcgttcca ccagaactca tccatcccct tcaaagtgaa
     5341 tacttctttt atgtttggga cgaggatcgt tccgaggtgc gttggatgac ctcatgggac
     5401 actaccgaac aggatattga ccggttttgc cagctacttg aagagcttat gcgaactgtt
     5461 aagcgatagt aaagcatcgg gtgttctgtg ttcagggggg ggatagtgtt tcgtgttata
     5521 ctaactccaa acatgtggta atcatttata tatgagcaat gaggattgat ttttatcaat
     5581 ttcaaccttt aactatcaac tgtcaaccat caaccatcaa ccatcaacta atatgttttt
     5641 tatatcttcg cagcgcaaag tttcatataa atagcaatgg ttaacaaaag gaaattgata
     5701 atcctacttt cggcaattct gattggtttc ttagcttttt cctttggtat ctacctgaaa
     5761 aaaaataata ggactctggc attacatatt gctgagtcat caaaatacgc aaaggttgat
     5821 ttgctgagtg taaacaatgc tcttactaac agcaatgagt attcatctat caatcgccag
     5881 tttgagcagt ttgtgaacaa atgggatttg gttggtgcaa ccttttgcat tgcccataag
     5941 ggtaagatta tttacgcacg tggttttggc atggccaata aagaggaagg cctggctatg
     6001 cagccctata atactatgcg tgtggctagc gtttccaaac ttataacggc tacagccgtt
     6061 atgaaacttg ttgaggaggg taagctgagg cttaatgaca gggtttttgg cgcagatggt
     6121 attctaaacg atgaaaagtt cctgaactat aaggataaga gggtagagga aataaccgtt
     6181 cataacctat taaaccactc gggcggttgg actccccggt ggggcgatca cctttttatt
     6241 aaagatgtta ttgctcgcga gcttcataag gatttacctt tggatgttga tgactatatt
     6301 gtatttgccc tatcaaaacg acttcacttt cagcccggaa gtcatagctc atacagtaat
     6361 attggatttg taatactgca aagggtaatt gaaaaggcct cgggaatgga ttatgagagc
     6421 tatgtaaaga acgagatttt taaaccatta aatatttacg atgcccattt ggccaataac
     6481 tgggatagtt taaggtacga taacgaagtt agatactacg aggtgccaga ggctgaaaag
     6541 gttatttcct ttgatggctc aaagcagagt gttctaaaat cgaggggcgg aaacgatgtg
     6601 cgtgcactgg gtgcagccgg aggatgggtg atttcaccta ttagcctaac ccggtttgta
     6661 atggctgttg atggcagaaa cgattttcct gacattctct caaagcagtc tgtcgatatc
     6721 atgaagaaaa catacaatgg gaagtttcag ccgttgggtt ggcgttgggt tagggacgat
     6781 ggaacgctat ggcgctcggg ttccatggca ggcacatcgg cacttgttat ttcgcgcccc
     6841 gatggctaca ccttaacatt tacttccaat cagagtcact ggaaaggcgc ccgtttccct
     6901 tatgttgtcg ataggttctt cttgaggatt tttaacaagt atattaaatc cattccagac
     6961 cataacctgc tgaatgtaga taaaggaaaa tattagttta ggctaaaatt attttccagc
     7021 aatttttcct gcaattggat atgctcaatg aaccttagcc tttttatcag gtagttaacc
     7081 atttgcttct cgtttcgtag ggagtaaaat gcgaattttt cgtcagaatc gagttgtatg
     7141 gcttttgcaa cattcataag gtttactgag tccccagtaa taagcgagcc cattataggg
     7201 tcaaggttaa ggttcagctt cttaacaagt actaccagct ctggattggt tgtagtgaac
     7261 ctatcgtcaa tatactcaat tattccacca ccataaagtt tatcgggtag tttctcctta
     7321 aggtttaaaa ggtgaaagtt tgcaattgcc ttaataagaa taaccatctc acctagacta
     7381 ttcttggcta caatgttaac cagttcaacc tcgctaccgt aggatttaat attgttgtta
     7441 ctcacgtatg gtatgccaaa ggtgtctcca gtttcattgc attcattaat caactgcttg
     7501 taacggggtt caaagatacg caacggaatt tcctctcccg gtaaaagaaa caccgggagt
     7561 ggaaacattg ccatatattt tgtggtgctg cccatgaact attctttttt gttaggtata
     7621 aattttaacg aagccgagtt gatgcagtac ctaataccgg ttggctcagg gccatcgttg
     7681 aatacatgcc ccaggtgtgc accgcaattt ttgcaaagca cttcggttcg caccatacca
     7741 tggcttaaat ctttctgaaa ctttatattt tctacaaact tgctatcaaa gaaacttggc
     7801 catccgcagc tggaatgaaa ttttgtgtcg ctctggaaaa gttcagcccc acagcaaacg
     7861 catacataca aacccggttc aaaatgattc cagtactcgc cagtaaatgg cctttcagtt
     7921 cctttttgct gggttacgta aaactgtata tcggttaacc tattacgtaa atcatcactt
     7981 ttcatagtac tatccttaat gttttgagcg caagcgctta atgagattac tgtaatcaat
     8041 attgatatgt aagttttcac tttgtttagt cttttggtaa aaacaataaa tagtctcctt
     8101 ttgttcgacc gataaaaaag gttaattgct atttggaatg gaatttgatt gctatttttg
     8161 acaatatgga ttaaaacaaa taactatgaa aaaaataatt ctagccctga gtgtttcact
     8221 ggtatcggca ataggttatg cacagcaaac cgcagtttac tcgttaaagg agttcgataa
     8281 acttcgggtc tggggtgaat ttaaggtatt tctggttaaa ggcgattccc ttaatgccaa
     8341 ggttgaaaca caaagcattc ctgcatcgga cattgcgcta gatgtaaatg gcaatacact
     8401 tgaggtaaag ctcaagggaa aactttatga tagggtaagc gccactgttt acataaccta
     8461 cagggaatta agagagatat cggtatcggc agcggctgca gtaagccttc aggataccct
     8521 caaagcaact aatatttcaa ttaacattaa taccagctcc gagtttgatg gcgcagtatt
     8581 ggctgagact gccgatttag ctgttggaca gggctcaacc ctccgcataa ggggcagtgt
     8641 aagcagctat gaggcaaaag ttaacaccgg aggagtttta acggcaattg acctaattgc
     8701 tcccaaaaca tacgtaaaag taaatacagg tgctagcgct aaggtttacg catcaaaggt
     8761 tcttgaagct aatgttagta ccggtggtag cctaacctat accggaagtc ctgaacagaa
     8821 aaagattaaa acactggttg gcgccaccat tattgagcag taaatttaac gtttattgcg
     8881 ccattcaact atttgggcag caatgctcac ggcaatctca gcaggggttt gtccaccaat
     8941 gggtaatcca atgggtgagc aaaccctttc cacattctcc ttgccataac cttccgcaat
     9001 aaggctctca aatatggttt tcaccttgct tttgctgcct atcataccaa tgtacttcaa
     9061 aggtttaccg attagctgct ttagtactat ctcatcgaac ttatgcccaa aggtcattat
     9121 cacaacgtaa cttctatcgt ttccaggtaa aaggtgtgat acctgcttgt agtcaacaat
     9181 ctgttttttg tgggcccaga cattaccctc aaaggtgctt aaaccctgcc tgttatcgaa
     9241 caattcaacc ctaaagtcaa gcagaccgag aatttgcgaa agcggaacgc ttatatgacc
     9301 ggcaccaaat atgtaaacag tttcttttac ccctaaaggc tcgctgtagc ggaagcttga
     9361 atcgctcttt tgaaactcaa ttcctttaat gctaatatgc gattcaccct cgtatacctc
     9421 cgcgccactg gttgataaaa caatttttcc cagacggttt tcactttcaa gctcaataac
     9481 tttagctaaa acctctgaat cggcttttga aaaaaggctg aagatttgtg tttgctcgcc
     9541 ggaacatatc atccccgatt tatcttcagc tgcatcgtgt gagtgaacct gccgcttcaa
     9601 gaagttttgc ttatatcctt ctgccaggtt cttgcgtgcc atttctacca ggttaaactc
     9661 cattacacct ccacctattg agccgtaaag gctaccatcg gcggcaacag ccattttaaa
     9721 cccaaccttg ccagggctac taccctgtgt ttcggcaacg gtaattaggt aaacgtcaat
     9781 attctgctca atttttgaac ttataaattg ccaaatcata cccatgttta attttttgta
     9841 aatttattaa gatattctct aaggattttt ttaggcaaaa atatctttct atttagtcat
     9901 aacgaagatt aaagacataa taatattgta atatatagaa aaaatatcta tatttaaatg
     9961 gtatcacaaa aattatcaat actcattgct ttaatgtgta gaattataaa taattttgag
    10021 tactaaaatc tacataaaat gagcgacgta tatagtaaga taaaggagtt atcggagaaa
    10081 tatgccgatt atactgcacg taatctgtcc cgattggttc agattaaatc cttgagtgcg
    10141 caggagcgcg acgtgcagct ggagctaaag aggcaaatgg aagaggctgg ctttgacgag
    10201 gtttttatcg atgggcttgg gaatgtaatt ggtcgtattg gaaatggaaa acgtattctt
    10261 gccatcgatg gccacatgga tacggttgat attggcaata aggataactg ggagttcgat
    10321 cccttgggag gcgaaattcg cgatgggtat gtacatggac gcggaacagt tgaccaggaa
    10381 ggcggccctg ctgcatttgt gacggctggc aaaatactta aggaacttgg ttttaacagg
    10441 gatttaacca tatactttgt aggtagcgta atggaagagg attgcgacgg actttgctgg
    10501 aagtacattg tggaggagga taaaataaga cccgattttg ttataagcac tgagcctaca
    10561 aacctcaata tatacagggg tcaccgcggt agaatggaaa tgcaggttca tttttacggt
    10621 gtttctgctc atggctcagc acccgaaaga gggaaaaatg ccatttacat ggcctcaaag
    10681 gtggccttgg aggtagaaaa actaaacgaa cgactaaaat tcgatgagtt tctgggaaaa
    10741 ggaagcatca ctgttacgga gttcacctcc agtagtcctt cgctatgcgc agtttccgac
    10801 tatgcccgca ttcatctaga ccgtcgcctt acctggggcg aaaccaagga gagcgctgtt
    10861 gccgagattg aagagctgat taaaggcatg aatgccaagg tggaagtgct aaactatagc
    10921 gagaaggcct atacaggact ggaatacggc atggaaaaat actacccaac gtggaaaatg
    10981 cccgaggata gtgagattgt gcagaaaggc gtaagcacct accagaagct ttttggcaat
    11041 gctccaaagg ttgataagtg gaccttttcc acaaatggta tcatgacctg tggaacctat
    11101 aaaattccta caattggttt tggcccaggc aatgaggtgt tagctcatgc tcccaatgag
    11161 aaggttccca ttagcgattt ggtggcagct actgcctttt atgccgcctt cgcatatgag
    11221 atttaggttt cgtattgcac aaattaaatt gtgaatcagg aatcaaagtc gtttagttaa
    11281 ggttaattgt catgttgagc ggagcgaaac atctcaaaac aatagattaa tcgagatcct
    11341 tcgtttcact caggatgaca aagggagagt ctgacttatt atccaaacga cattgattca
    11401 ggtttttcga ttgttgtatt acaaaatatg ctgtaacttc attttaaatt tgttacccac
    11461 gcctaattag ctaacatagt taaaaccgaa taaaaacttt gaaatatgga tttaatcaag
    11521 aaaatagcgg agttaaatca ctacaaatca gaccttagcg gtaaagattt tttactcaca
    11581 tgggagaaga gcgacgacga ccttaaggtt gtgctacagg ttgcttccat tcttaaagag
    11641 atgcgtaagg aaaacatttc gccccgcata ttcgattcag gcttggctat ttcaaacttt
    11701 agggataagt ccaccagaac tcgtttttcg tttgcctcgg ctgcaaatct gttgggactt
    11761 gcagttcagg agttggatga ggagaaatcg cagatttcgc acggcgaaac cgttcgtgaa
    11821 accgctaaca tgatttcgtt cctttccgat ttcattggta ttcgcgatga catgttcctg
    11881 ggtgaaggaa acaagtatat gcgccaggtt ggcgaagcac tcgatgaagg ttttactaag
    11941 ggggttttgc ctgttaggcc tggtattgtg aacctgcaat gcgatatgga tcacccaacc
    12001 cagtcaatgg ctgacctgct tcaccttgcc aatgagtatg gttcactgga tgctttgcgt
    12061 ggcaaaaaga ttgtgatgag ctgggcatac tcgcccagct atggcaaacc actatcggtt
    12121 ccacagggta ttattgctct gatgacccgt tttggcatga atgtggagtt agcctaccca
    12181 gagggctacg accttattcc tgaaattgtt gaaattgcca gaaaaaatgc cactcaaagc
    12241 ggtggtagct ttaaggttag ccacagcatg accgatgcca tgcgcgatgc cgatattgtt
    12301 tacccaaaaa gctgggcgcc attccacatc atgcagcaac gcacccagct gcttatgaat
    12361 ggcgataaga atggccttaa agagctcgag caggtttgcc tggctaacaa tgccaagtac
    12421 aaggattggg agtacaatga gcaggttcgc aaggtaacca ggaattccga tgctctttac
    12481 atgcactgcc taccagccga tatttcgggc gaatcgtgca ttcagggtga ggtaagtaag
    12541 gaaacctttg agcgttaccg cattaaaact taccaggaag cgggtttcaa accatatatt
    12601 attgctgcta tgatgcttac caataggttc caaaatcccg ctgatattct aatgaaactt
    12661 attgaaagga acacaccaag agttataaag tagtagccca ttttatattt cagcagcacc
    12721 ttaaggtgct gttttttttt gctttattat gtagtttaac agaataaatg ttatactaaa
    12781 atatgccttt cccttcaggt atatctatta gaattatact tttgactaat catttgtttg
    12841 aatattctaa agactaaata attattgttt ataatttcta aaaatgatgt gaatgttggt
    12901 attaaactat gccatgtatt atttttattg tgataaaaag atataaaaat gaaagagttg
    12961 atagaggaga tattcattga tgcatgggct atcaatgata taattagctg ttatcaggat
    13021 aaaaaagccg attaccccga tttatttcag atagttagtg gttatgacct gtcgaaacct
    13081 accaacaaaa ttcctttcaa ggtttacgtg gagctatgta gctggattga aaagaaactg
    13141 ggacaattta acctaattaa aattggccgc cggattggtg aatcaaccta cagtctaatg
    13201 cttgaaacca acatggttaa taccgagagt aagccgttgg atgtaatgaa agcattggtt
    13261 ttgctagcgc agaaaggtgt aaaagatccg aaacggagag gttgggaaat agtttcacac
    13321 actgataagt ccattcaaat gcgaaaaacc caactattta acacgcatat tcaggtgggg
    13381 ttgctcgata ccctagtgag aaaatgcaag gtgtatgggg ttcaggttaa cttagttaag
    13441 gaggaaagca aaggtgctga gtttgatgag tatcttataa cttggcttta attaacctgt
    13501 ttcaaaccct accacaattg taggaacctg aattatccat tcttttgtag tagtggcctg
    13561 ttgttttcgt tgatcggcgt ttaagtagtt gaccgctggg cagattttcg ctcaattttc
    13621 cgttatcaac tgcaataatt ccgtttttga ttacgaaagc aacctcacca gtagtctcaa
    13681 agccttcata aatgcttatg tcggactttg agtgatgttt acttgaagaa attgagcctt
    13741 ttacgtttgg attccaaatc acaatatcgg catcggcacc aatggcaagt tcaccctttt
    13801 gagggtaaag tccaaatatt ttagccggtt gtgttgcaaa aacatcaacc aatttgctaa
    13861 ggcttagctt accggttaat gcaccaaaag tgtacagtag cgcaagccta tgttcaatcc
    13921 cgcctgctcc gtttggtatt ttgcggaaat cgtccttgcc caccatcttt tgctcaagac
    13981 tgaaagggca gtggtcagtt ccaaccgtta gtatattacc attgttgatg gcattccaga
    14041 gggtatctct gtcaatactt tgcctgagcg gtgggcttat aacatacttt attgcttcgg
    14101 gcaaagtgga atcgtacagg gcttcatcga gtagaagata ctgagggcag gtttcggcat
    14161 aaagcgattg gccacttttt tgggcttgaa taatatgttt aaccgattca gccgccgaaa
    14221 cgtgaacaat gtaaaggggg cactgggttt gatcggcaag gtcgatagca cgtttaaccg
    14281 ctaacgcttc aaatcgaggt ggacgcgaga gcatgtggta ctttggagct gtaagaccct
    14341 ggctggcata aaaatctctt aaagtatcga tatcatcgcc catctcgcag tgagcagtaa
    14401 ccataccacc attctgagcc actgtatgca ttacatggta taaggcattg tcatcaattc
    14461 caacggaact tttataggcc atgtaaactt taaacgatgg aaatcccatt tcaatgcatt
    14521 cctcaatctc tttgggagta tttttattcc attcaatagg gctcacatga aatgtgtaat
    14581 caactagcga gcaagcagcc tcatccaatc gtgcttttag cgcttcaacc attgattgac
    14641 cccgtgatgg ggtcacaaaa tcgataatgg ttgttgttcc gcccataagg gcagcaatgc
    14701 tgcccgaatg aaagtcgtcc gatgaaaaac cggcgggtgt aggtaggtgc aaatgaacat
    14761 gtgggtcgat acccccgggc agcaccagca taccctgtgc gttgattgta gtttcagcat
    14821 tagcttctag ctggtttcca atggccgcta tcttcccatc aacaaccaac aggtcggcaa
    14881 tttgctcgcc aaccgatgtt acaatcctac cttttgtaat taatattctc aattcctact
    14941 cttattcaag tattttcaga aaatggttac cgcccgattc agtaaagcct aaatgttgca
    15001 aatatttttt gtggctctta ttgcatgcct ttgcccacac ccgggtatac ccgctttcgg
    15061 taaatttctg gctaagccag tagtaaacaa acttgccatt cttaaagtcg cggtattcag
    15121 gtaaaacgta atcaagctct acctcaagcg cttttgtttc gttgcgttgg gcaatgaata
    15181 ccccagcaac tgccatgtta cgcagaatga ggaagctaac tgtattttca gtgggggtat
    15241 agacaaaacc aggaaagaaa cgatgtatat ccttttgatg gaaatcgagg aaacgcatca
    15301 gataacggtt ctcggggcga acctcaagta tctcaaacac ctcttttttc gagtagattt
    15361 gaaataggta gtaagcatcg gtaagcacaa taaaaccgtt taaaatgccc acaggccatg
    15421 ctccaatcag aaaaccgtaa attgagaaca tcaatgctcc tgttaggttt atccaacgga
    15481 acttaactat cgaactcatt gtcattgata gcgcaatgat taccgatgcg ctatacccaa
    15541 tccattccag tacttgttga ttcatctaaa tttgttttaa agggcaaata taagcataaa
    15601 aagaggaatt tattatttgg taattgttgg ataactaaaa tgaaaagtat gactatttag
    15661 tgagtttttt cgagcaacaa tcaaccatcg gttatcaatc ttttctgtca tccttaacat
    15721 agcgaaagaa catattctta gtttacagtg ttcagtgtgc agtgtacagg ggtgattgtt
    15781 tttagtgttt agtgtttggt ggttggtggt tggtgtttgg ttaatttaac ctgtgtaaat
    15841 caattcaatc agtgtgatct gcgttctnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15961 nnnnnnngtg tttggttaat ttaacctgtg taaatcaatt caatcagtgt gatctgcgtt
    16021 ctattttgtg acaatcaacc atcaactcca tttgtcatac caagaagtgc gcaggatctc
    16081 atgcaacgcc aagtggaaaa atgagcaaag agataccatc gtgagctcag catgactgat
    16141 tgacgtttcc gcaaaatcta tcccaatatt tcaggactag acaaccttta accatcatcc
    16201 tttaaccatt atcctttaac ctttaacctt taacctttaa cctttatcct ttatcctatc
    16261 cttcccctct catccttttc caaagctcta gcgctaattt ttgcgattct ttaagaatga
    16321 tttcctgatc aacggtggtt atcgctctgc ttttgactat cagttttcca ttggaaataa
    16381 catgttctat atgcgactgg ttgataccat aaataaagtg tccaggaata ttttcagcgt
    16441 taacgggggt gggggaatcg taatcgagaa ccacaaggtt atcggggcca tcgccgctga
    16501 agccattttg ggctatgtag tggtgagcat ttctaagtcg tttgtatgct tgcgcagggc
    16561 tcatggggtc aaagttttgg ccaacaaagt aagccgattt catgctttgt agcatatcgc
    16621 tgtgcatacc atcggtgccc agcatgatat tttcacccag atttttactg ctgaaatagc
    16681 caacgttgtt gttcaggtta ctatcagtgt tttgtgctac ccagcagggc gattgaccta
    16741 taaggttacg ttccttttcg gaaaggtgca ggcaatgcac cagaatcgat ttgctagagt
    16801 ttaggaagtt aaagtcgcta agcctttcaa ccacagtttt accataggtt tcatggcaat
    16861 gttgttggtc gtatttgtct tcggctacat ggatatgaac acccgaatta tacttctgcg
    16921 ttagatctgc tgctgcctgc aaggtcttat cgcttagggt gaacgatgcg tgtaggccaa
    16981 caagtccctg atagtttttc aggtagctat cggtttcctc aagtccttgt tgggcaattt
    17041 catcaccatc cctatcgcta atctcgtagc agagtaggtg cgataggcca accctttcaa
    17101 atgctttggc aagtgtctca agtgaacctt ttaccgcata aggggaggca tggtggtcaa
    17161 taacaaatgt agcccctgcc tttgcacaag ccatagcggt aaccaagccg ctatactcta
    17221 ccatttcggt ggtgaggcat ttatcgagtg tccaccagat atatttaagg atttcgtaaa
    17281 aattttcagg tgattttttg ggtgcaccca ttcctcgggc taatgctgag tatgcatggt
    17341 gatggccaac aataaacgaa tgcgtaacca actttccgga acagttgatg gtttcagtgg
    17401 cattgcccct gtatgttttg ggtggatgga attcaatatt accgttggta accagtatat
    17461 cgcaattctt gatttccagc gtttgatagt cgatataggt ggcatcggaa agtaaaatca
    17521 ttgggtaaga atttggtggt taaacattat tctgacttgt tccgcatcat gggtaggcgt
    17581 gttcgcttta taccgtcaaa agtccaaagc gcattggcta tggctgcagc ggttggcacc
    17641 atgcctattt cgccaatacc ctttgcaccg aatgggccga ttgggtcggg ctcttcaatt
    17701 ccaataactt caatttcggg catttggttg gcgcggataa tcccaatgtc cttaaactta
    17761 aagctaacgg gaaagccatt ctccatgggt aaatcctcgg ttagtgcata tcccattccc
    17821 atatgcacag ccccctcaat ttgaccctcg aaaagcatct gattcatgat tttgcccgca
    17881 tcgtgggcag caattatttt cgaaagtttg ccctgttcgt taagaatggc aacctgtgtt
    17941 gcatagccat aggcaaagtg tattaccggt ttatcagttt ttgcgccggg cttgcatgtc
    18001 caatcgcaaa caaatttacc acgataaacc cggcctacca gcttttcgag tttggccgtc
    18061 tttaaatctt ctctcagtgg acgcgcagcg ttgataaggg cgttggctac cagggctgta
    18121 gcccttgacg aagtggtcat accagttgga atctccgcat cggtatcaac tatcacctct
    18181 atacagcttg gttcaatacc agtttcatgg tgaagcgttt gaatggccat ggtgtgaacg
    18241 ccttgaccca tttccgtcca tccgtggtga atttcaaccc tggttgggct aataaccttg
    18301 attataacct cgctatcgtc aaccattccg ttaccgacac cggtattctt taggccgcag
    18361 gctatgcctg catactttgc cgacttaaat tgttccttaa ctgcaataag ggttttcttt
    18421 agtccaaccc catgtagttc ctgaccggtt gcagttttgg cgctttcctc aagggcgtta
    18481 tcgtagcgaa tttgccagcg gtcaaaacca ccctgacggc aaaggtcgtc gatacagctt
    18541 tcaatggcaa aggtaacctg tggaacacca aagccacgca tggctccaca tgggatattg
    18601 tttgtataaa cggttttagc cgagatatct acagcgggta cggtgtagcc acccgtggca
    18661 tgcccgacca cacgctccat caccttagtc cctacggatg catatgctcc tgtatcgcct
    18721 atggcctcaa gtttaagagc agtaaatttc ccctcggagt tgcaggcaag gctcattttc
    18781 atccaaaccg gatgcctctt ggggtgcatt cttatgcttt catcgcgggt aaggtgaacc
    18841 tttacgggca tttgtgttat gtagctcatc agggctgcat ggccctgaac agttaagtcc
    18901 tctttcccac cgaacccacc accgttttga acctgaacca cattaacctt actttcgggt
    18961 aggtttagga tttttgctat ttgctttcgg tcaacgtaaa caccctgccc ctggctgaat
    19021 acctttatgc catcgccatt gggtagggca agtgcagttt ctgtttccag aaatgcatgt
    19081 tcaatacgct gggtttcgta gatatcggac gaaacgtaag ctgcttcagc aaaagcctta
    19141 tcaacttcac caaaccgaat tgtgcatgtt tccaacacat ttgaccgatt tggatgtacc
    19201 tggggcgaat cgggttttat ggcctgatgg atatcggtaa caggctctaa tacctcgtat
    19261 tcaacctcga ttaatgaagc ggcatgcctg gcaatatcct ccgatgttgc caccactccg
    19321 gctataacat cgccgatgta acgtgtaacc tcgcccaggg caaccattac tggccaatcc
    19381 tgaaaaatca gcccaatgta gcgttcaccc ggaatatccg ttgcggttat tacctttacc
    19441 acaccctgca ccgacaaggc ttttgaggta tcaattttta aaacctttgc tcgtggatgg
    19501 tcggaaaact ttaaggctcc gtatagcatc ccatcaacaa acatatcgtc aacaaaaggc
    19561 ctttggccta gggctgtttc cttggcttga tattttggat agcgttcgcc aatcctggct
    19621 cctgtattat tctttggcaa agcttggcca tttagctcgg caaaggcatg ctgaatggca
    19681 tcaataattt tgacatagcc tgtgcatctg caaagattcg gcgcgatagc cctgatgata
    19741 tcgctgcgtg ttgctttggg gttcgatagc cataaccctc tggcacgcat tatcataccg
    19801 ggcgagcaga atccgcactg aactgctccc ttggtggcaa agtggtgggc taatatttcg
    19861 cggaaattat ttggcaaccc ctccagggtg tatatgatag cctcacttag gtcggacatt
    19921 ttgatacggc aagccatttt aatgctaccg ttaacctcta ccgagcatgc cccgcacacc
    19981 ccttgacccg agcagccatc cttagctgcg gttaggtgca agtcgttccg caaatactcg
    20041 agtaacgaac gatttatatc accgttatac tctaccgttt taccgtttaa gttgaatcgt
    20101 atcatagcat ttacaaaggt aaaatgtttt ggaatagggt agaagtcgca aaacagattt
    20161 caggattcta tacacaaaac tccgttaacc tatacctcaa ggatttcaaa ttctaaaatt
    20221 taatccattc gtttttcctc aacgaatcaa ttgtagggtc aatggcattt ggaatattaa
    20281 taaccgatga aacggctttg aggtctttta aaccgctacc ggtgagcaac acaactaccc
    20341 tttgctttgt aagcaatttt tgattctttt ggtagcatag cattccagca aaggctgttg
    20401 aagctgcagg ttcagcaaaa attccggtgt tacgcgcaag gatctttgaa gcatttacaa
    20461 tatcatcatc ggttactgag agccattcac ccttgtattg gagcaggtag tcttgagcca
    20521 taaagaaatt tcgtggaata tctacagaga tggagtcggc aagggtattg ctgggtttta
    20581 tggtaaaatc gggttttcca atattttcga ttagatttga gctgccggct gcctgaactg
    20641 caacaaccgt gggcatacga tctatcaacc ctattttgag caaatcttcg tatcccttgt
    20701 aaacccctga aataattaca ccatcgccaa cgggaacaaa gatataatcg ggtatagccc
    20761 tgctgagctg tttaaacagc tcaaacgaaa cggtcttttt tccctcaatg gttagcgggt
    20821 taaaggctgt gtttcggtta taccagccaa actctttggt tgcctccatg ctaaggtcaa
    20881 aagccatatc gtaggtgccg tttaccggaa caattgtggc tccgtacatc acaatttggg
    20941 tgagcttagc atgtggtgcg cttttgggta ccataataat ggctttttgc tcctgcgaag
    21001 cacatatgcc ggcaagggaa gagcctgcgt ttccggttga tgcagcaact attgtgctaa
    21061 tgccattttc cttggcatag gccgatacca cagccgatgc cctatcctta aacgaaaagg
    21121 ttgggttttg cgaatcatcc ttcaggtata gctcaaatgt aagcgtttca ccatcaattc
    21181 tatcaaacct atacaatggt gtttcgccaa cccttagctt gggtaacgat tcctttttaa
    21241 gcagcgggat tagatcccaa aaatttcctg tgattacctc ttccttatgt ttactcagga
    21301 tttcatagtt gtataatgct tttagcactc cctttggtgg gtttacagta tcgttattcc
    21361 tgctgcaatc agggcaaagg taaagctcag gcgatggcgc aaaggttttg ccacagcccg
    21421 agcaggtaag tatgaacttt ccgttcataa aaaattgatt taaagcgatt ttagcagacc
    21481 aacccgatcg ttaaattcat caataagttc gtcccaccgt ttaacctggc taaagagttt
    21541 aggccaaagc tccctgtcgt tccagagcct gttcaaatcg tcaatttctt tgccctgttg
    21601 ttcaacccag gtaaagtatt tcaggttatg aatggccttg cggtcgtggt aggttagctc
    21661 cctcatccaa tcggtagtag cacctaggat acatttctca aaatccttta tggcctggat
    21721 ttggctatag cttccgcgtt cctcccttaa ctcctctagc ctcgatccat acatctcggc
    21781 cgaatccgtt gctatggtga atatcacatc ctctgaagtc atctcaaaga atttggctgt
    21841 tttaatagcc gagagcatat tgctaatgcc cgatataccc aaaagtttca ggttgttggc
    21901 aatttctaca ggcactcccg attctacaag gtattttaca ccttctggtt cattgaagag
    21961 cctgaaaatt cgcatgcaat cctcatcgtc gatagcggtt ataacatcgg tgttcttaac
    22021 attatgaatc cagggtacat gcttatcgcc aattccctcg atgcggtggc cgccaaagcc
    22081 attcatcaga atggtagggc actgctttgc ctctgaggct accaccttga tgtgtggcgc
    22141 tatggttcgc aggtagtcgc ctgcggcaat ggttcctgca gagccggttg ccgaaacgta
    22201 cgcggcaaga tttgactttc cgcccagctc gttaaatacc tcctcaatgg ctttccctgt
    22261 gatattatag tgccatgcgg cattgccaaa ctcatcgaac tggttaaaaa taacgtaatc
    22321 gggttttgtt ctacgaattt cccagcactt atcatatatc tccttaacgt tcgactcgca
    22381 accgggggtt gcaataacct cagatcccac atagtcgcga agccaggtaa agcgttcacg
    22441 gctcatctcc tcgggtagta ttgccaccga ctcagtaccc attagcttag aatcgaatgc
    22501 tccgcccctg cagtagttgc cggttgatgg ccacaccgct ttttgggttg ttgggtcaaa
    22561 gccaccagta ataatgcggg gtgcaaggca tccgtatgcn nnnnnnnnnn nnnnnnnnnn
    22621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22681 nnnnnnnnnn nnnnnnnnnt atgagaaata ttgacgaaat agaccgcaag cttctgaaca
    22741 ttttgcagga caatagccgt gttaccattc gcgagctttc tgaaaggcta cacctttcaa
    22801 caacacccat ccatgagcga attaaaaagt tggaaaagaa tggttacatt aagcggtata
    22861 tcacgctgct cgaccctaag gcattaggaa aaaagcttac ggttttcatc tcggtatcgc
    22921 tttcgagtca caccaaggag gtggttgatg cctttgagca tgaagttaaa aagttacccg
    22981 aggttatgga gtgttactac gtttccggca actgggattt cctgctaaaa gtgcagtgta
    23041 acgacatgga ggactaccat aactttgtga tacacaaatt ttcagtgata aagaatatta
    23101 ctcagtttta cagctcattt gttatgtccg aatcgaaaca aacctacaag tatgagttgt
    23161 agatggtctt tataagttga actattttta catactgatt tttaagagtt taactatttg
    23221 ctgttaggca aacctcttga ttttagggtg cttgagtaaa cctcaattat tctattggtt
    23281 gttttactta ttccaaagtc gttttcaatt ctcgctgcta ggattgacgt gttttttcgg
    23341 tattcatttc ttctttcaaa gtaaagctta agttcattat atatcgattc ttcagtagga
    23401 acacagccat caattttaat aaaattggta aatgcatagt catcataatt gcttggcgta
    23461 ataattcttc ccattttgtt gttttttatc gaaattacag tagcccctcg aataagtccc
    23521 tcaatagcta cacgtccaac tccaagtata agttccggaa aactattcag taaatcaata
    23581 tttctaacaa aacccaagta ttcaatagca gtactttcga attttaaatt ggttttgttt
    23641 atcagttctt ttaactcttc tacattattt ccatcgccaa ttaataatag tttaatggta
    23701 ggttgactta atttcaattt ttcgcaggca agaataagca gttttattac tctaaaatgc
    23761 cttttatcaa tcctacagat gtatccaata gtatttgttt caatgagtgg taatttttta
    23821 ggaatcggaa tgccattagg tatataaact gatttgtttt gtatagacct aaacctgtct
    23881 aagttggaaa ttgcctgatt tctcacaaag attatttttg atgttaattt ttttgctact
    23941 atcgacctta aatcgtgcct aacgcggccg tgaacggtag ccaccactgg gactcttagt
    24001 ataaatcccc ctatgcatgc cgaaattatt gggagtcgtt ggtgtgcatg tatcacatca
    24061 atcttatgcc gtaatagcag gtaaactatt attacaacct ttgtcagtat caaatagctg
    24121 acaaacgaag gtagttgtat aaattttact cgtttatcca acagctcatt ttgtcgtccc
    24181 tttcgggaaa gcagaataac ctgatggttg cttgctgcaa gctcgttaca tagctcacgc
    24241 acgtggttct ctgctccacc aaaatcgaac cgagtaacaa gctgtagaat acgcattaat
    24301 ggtaatttct gataatttat ttttttgaat cttctctaaa ccctttggag attagtccag
    24361 tttaaagggg gtcaatacac aaccaccaag cactaaccac taaacactta acactaaaca
    24421 ctaaacacta aacactaaac actaaacacc aagccctaat caaaccccca ttaacctttt
    24481 tgcaccctgt agtattacgc tcatctctac cgcatgctga aattgaattt gtcttacgca
    24541 aggcgttaac gccttagcct caaccagctt aaagccattt ttactgaaac gggcgtaaac
    24601 gtggtcggtt tcgtaaatgt attcatcacg gttttccgat agggtcttaa tgcctccttt
    24661 ttgcttaaag attagtacat ttctgtcctt cagaacgcta agcatgtaac catcctcgct
    24721 ctccttgaac gattttgtgg ttaggtgaaa cttaggctta tcctggtagg gtgcgccctc
    24781 ggttgggcag aacgtagagc aattcccgca ttcgttacag aagtttgcaa tattcagtat
    24841 ctgaacggtt tgttttacct caaaaaaatg gtcgggttga acctcttgtg ttccatcgga
    24901 accaaccagc actttgtcga gttggtaacg aacgggttca atggtatacg caaagttagc
    24961 taggttgggg caaactgttg tgcatatgct gcaaacctcg tcgcagtgta ggcagcgtgc
    25021 gctttcctct tttatggtcg tttcgtccgg agtttcagtc accagctcaa aggttttgct
    25081 cttttcagca gtgagctctt tatgtacata accatacttg cgggtggatc gcaggagcat
    25141 aagatcccta taattatgtt tcttttcagg gacactagta ctaattcccg taaaaccgca
    25201 ttggctgata atctcctggg ctgccttacg accatcgcca atggcattta tggccgttga
    25261 tgcaccacgt agcgcatcgc caccaatgta aacgttaccc atcaacgttt tatagttgcg
    25321 ggtatcggcc tttaggagct catttggaac aaaatcgata tcaagggctt gcccaatggc
    25381 gggaattacc acatcgcact caacctcaaa ttcagaccct tcaactttta caggctttgg
    25441 tctacccttg ctatcgtaac catctagctt cattaatcta catttcaatg ctttaacctt
    25501 gccgttctgg gtgattattt tttcaggggc ggcaagttcc actaactcaa caccctcggc
    25561 aattaccgct ttaatttcac cctcatcggc aggcatttcg ttaactgttc ggcggtaaac
    25621 tatggttacc tttccgttat cgcccacaag cctatgcgct gttcgggctg catccatggc
    25681 ggtgttccca ccgccaatta ttacaatacg tttgccaatc tgctttatgt taccgctttt
    25741 tacacccatc aggaaggtta gggggtcaat aacaccatcg gcatcaatac cttcaatatc
    25801 aagtatagcc gatttctgcg caccagccga tatgtaaacg taatcgtact tgtttttaag
    25861 gtcgctaaac tcgctgcggg ttacggggtg gctgtaatga atgtttacac caagttgggt
    25921 aatcctttca atatccctat caatggcctg gtcggttaag cggaagcccg gtatggcata
    25981 gcgaaccatt cctccggcct tggtcttgct ttcaaaaaca tcaaccttaa agccattaat
    26041 ggccaggtaa aatgcacacg acaagcccga tgggccggcg ccaattatgg caaccctttt
    26101 accatttggt tgagcaggac ttagcttgat atcagcattc tcagcaacgt agcgtttcac
    26161 gtcgcgtatt tgtaatgggt tgtcgtagtt aatacgggta cacttgttct ggcaaaggtg
    26221 gtcgcacacc attccggttg tgttcggaaa tggattggtg cggagtatca cctcaagcgc
    26281 tttggggtaa ttgccagtgg cagcaaagtg aaggtatgtg ggtatttcct gattggtagc
    26341 acaggtattc acgcatgggg ctgcaataca gtcgaagtag tcgagcttac gcttggtttt
    26401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ttaaacgcat caactcctgc
    26521 cgagaacgat agaagcaatt ggcctttgaa atcgttctgc agcttgcggg caaggtttat
    26581 ggaaatgggg tgtaatgccc ggccgctcat gtacatcatg tccacctcgc ctgggaaaac
    26641 atgtttattg ttaacgcttt ccagcgtatt ggttagcttg accccaaagg taaggttaag
    26701 ggtttttgct tcggcggata aggcttcaat cagctttagg gcatcgggat attttaggtc
    26761 gtgctcaaag gcgatatcgg gaacgttggt tttgaacctg agcttgttgt tcagagtctc
    26821 tctaagctca gaacttccca aaagggtagg gttgagctta accagcgtat gcaatctccg
    26881 ttcagttagc aggtagcggg ctatatcctc aatctcattg gccggacacc cgtgcatggt
    26941 ggaaagcgta atattatccg aaagcttagc atgtatatcg atgtttccaa tggctgggta
    27001 tatggctttt agctcctgca ccatctggtt cagctcatcg gtggcattac ccattttatt
    27061 caggaaccac tgaacgttgg gttttttaat accctcaaga ttatagccta cgctcatgtt
    27121 gaacaccatg ttgggcttac tgcccagctg cagcttatgg ttcaggatgt gaatgattac
    27181 ccatgccttt aggtattcca taaagctctc ctcaaccctc agctcctgcg accactcaca
    27241 gttgtacccc tcatcctgca tgtcgatgca gggttttgca atctcaagct catcgagggt
    27301 ttgaatggtt ttcagctcaa ggtaccgggc tcccatgagc caggcaatta caatgttgtg
    27361 agcaagttga gtatgtggcc ctgcggcaac acctattggt gtatcaatgg tttgcccaaa
    27421 aatggttgtg ggaagtttac cttgattcgg gttaaagaac agttcgctgg ggtatccaaa
    27481 aacggttcca tgggtatcaa gttctttcag aatgatttcc agcaactgtt tggccggtat
    27541 gggtgaaaac ttatcgctca tatatcctga tatttcccac aaatctataa aatatggcta
    27601 agcatattaa ctttgtatag cattttattg ctatttggca aagaatatta ctatacaagc
    27661 gaatgaaaga aaatttttcc gccattattc tggcagccgg ttcctcgggg cgaatggggc
    27721 agcctaaggc tttgctccca tttgacccgc aacgttgctt tgcccgcaag cttgccgatg
    27781 agttccttgc ctttggctgc caaagggtgg taatggtaac caatgcccag gtagccgcac
    27841 aggcagtttc cgcagcctat aagttaaacg gtattgaaat tgtgattaac ccaaaccccg
    27901 agctggggcg tttttactcg ttttaccttg ccttacaggc aataggcaac cactatgttt
    27961 tcatgaccaa tgccgataac ccctttgtgg gtgctgatat cctgcgggca ctatggcgtc
    28021 atgcaatgca agccgactat gtttacccgg tttacgaggg taggggtggc catcccatac
    28081 taatttcacc acgaattgtg agagcagcac gagccgagac taacccggat atcaacctca
    28141 aagagtatct aaaccgattt agcaaggctt gcatacccgt tcccaatgat caaatcctta
    28201 tcaacatcaa cacccccgaa gagtatacca ggcttattgc cgatgggaaa ttgccaggca
    28261 acgaaatttg agatgctcag aagagtgaaa tctttggggg tttaaaggtt gggtttatga
    28321 aaggggtggg agtggaaaca ggttgcagtt tggcaatagc acgggaatta aaagtgcaag
    28381 actgtcatat tccaacaaat gaatgaaaaa aaaactacat ttcgaattag tcactgcacc
    28441 ctgcaatgtg ctaaaccgat gttggcaact ggtgttctgt ctatccgtcc tcatgaatca
    28501 ttgtcagtgc atggtttgag attgctgttt gatcttctaa attataattc ttaagtattt
    28561 ccaaatcatc tatttttact tattgtgaag caacttgttc gggttgataa tgggtaaaac
    28621 aaagttttgc aaagccgtgc cttgcagtcg ggttataaga atccctcttg atgtagaata
    28681 ggttacagat gaaagtgcat ttgccaaacc cggatttaga tttaaacgct tatttttttt
    28741 cagtatagta agctcttcta aattttcaat tctgtaaata aatattaaat ggaaatttcc
    28801 tgttgcttcg ctttcatttt cgcccttgct gtcggttttt acgttaacag tgaaatctgc
    28861 tttagccaat ttatcgtcaa ggttaaagct caattgcaag gagttgtcca aatggtatcc
    28921 gacaactttg ttaatgtcaa aattttcagg agtgttcacc tgtcctttaa tcattttgaa
    28981 atcaatcaaa gcaatctttt ctggatcaaa aacattttta tcctgcattg cttaattcat
    29041 tatagggttc agtttcttcg gcacattgcc aaacattttc agtggttttt gtgtttagtt
    29101 ttgtataact cacaaaagtg tgaactctgc acacaaagcc atcttcaaca aattctgtct
    29161 gtactttttt cttcgtcact tctattaaag gtactcccaa ttcagccgat aattttgcta
    29221 gtgaacgtaa ggtgaaattg tgctcaccgt ttagccattt ggaaatttca gatggtttct
    29281 tattcatctt tacagacagt tcctttttac tgatattttt ttcacgcaat agttgattaa
    29341 tgcgaaccac taagtcagca taccaatcca caaagatttc aacatcttta ggtgtgcttt
    29401 tcagcaatct atctactgtc ttgcttctca ttgtcttata gtattatgtc agttgtcccg
    29461 tcaaagtttt gtaaatacct accgttatca gagattttaa tcatttgaga ttgtaatgct
    29521 tcttcaattt ttttgacaaa ggtttgtgct tcataaaact tcattcttaa atttttgctt
    29581 tcttgtgatg cttgcgattc cttaatccca ccgttgaaaa gaataacaat attttcggtt
    29641 acccgataac aaaataacct cagtggaaaa ttgatgccta agtcttttat ttcttctacc
    29701 cattgttttt gttttggagg caattcttgt gctttgttta ctattctgtc aaaaaaatca
    29761 tttgttgctc cgtatctgtt gccaatgctt tcggttatca atcgaaacaa ttgcattgcc
    29821 ttttcattta atggatgctc aggcacagca taggtatcaa aaaacctatc cgtttctgat
    29881 ggtgcattgt catcatctgt aacccacctg acggtgtaga atgtgcaaat gcttccttca
    29941 tcataccata tttcgattgc aaatgtattc acttataacc gaattatgca attttttgtt
    30001 tcacttttaa gttaaattat tcaaaataat tacttaaaac cctatccttc aatatataca
    30061 ttcctatttg tcagatcatt agtttgatat taaataaaaa attcatccct cgctcttgtg
    30121 cgtctgcgga cgcgtgcaat atacgccact ccgtacgata actttcagta aatgtagtaa
    30181 aaaatccact tgttcaagca agtacaaaaa cttttttatg tccggctgaa acgctgcgtt
    30241 aggcttatac tccatttaac atcgcttacg cgcgctaaca agagcaatta tggaaaactt
    30301 tgttttcact tttatatgct gttaggctac ttcttaccgt tttctattcc gcttatttgt
    30361 aaattaggtt gaacaatttg ttggataaat ctttgtccaa ttctttgaga attttgtaca
    30421 cttcaaatgc agaactctta tctcctacat gcaaataagc cactcccaga ttatagtgta
    30481 ccattgccaa atcaggcttt aagcgaatcg cctgcttgaa agcttcaacc gcttcggtgt
    30541 agcgcccgag tttgtcataa gcagtaccca ggttgttgtg tgcttctgca tcatcaggct
    30601 ctatgcgaat tgcctgcttg taagcttcaa ccgcttcggt gtagcgcccg agctggccat
    30661 aagttaatcc cagattaaag tgtgcatctg cataatcagg ctttaagcga atcgcctgct
    30721 tgtaagcttc aaccgcttcg gtgtagcgcc cgagattgac ataatccatc cccagtttat
    30781 agtatgcctc tacataatta ggttttaagc gaattgcctg cttgtaagtt tcaaccgctg
    30841 cttgtgcacg gagaaaagtg ggataaaaaa gaagaaaaaa aacacaggca tcaaaaagta
    30901 cttcaatttc ttcatattat taacctccag tattatcgtc taactcgttt atatcctaaa
    30961 aaatcattct tatacatcta atttgggtgt atagatctga taattgaaag acttatacga
    31021 tagctaattt aaaaaaaatt acaaatcatc aaacggacct ttaacttttt gcttactatt
    31081 ttacctaacc aatgaagtcg ttcgttgatg attgatagtg agcttaatag aacgcagatt
    31141 atgctgattt attggattta caaagttttg gattaaccaa acactaaaca ctaaacacta
    31201 aacaccaaac cctacctccc ctggtacaga ttcattaaaa ccctttcagg ggttaggggt
    31261 gcagtgagct ttatgctata gtgtgggtta aaggccttaa tggcattgtg aatggcgaag
    31321 taggctgcta tgccgtacat gaatggtggt tcgccaatgg cttttgaacg tagtactgcc
    31381 aattccgggc cattggtggg cagcgggatg cactgtatgc ttttgggtgc aaagtgaata
    31441 tcgggtacct tgtaggtgga taggctattg gaaagcaatc ggcccttgga gtcaaaggct
    31501 aactcctcaa gggttagcca tcctatccct tgcactacag caccctcaac ctgtccaagg
    31561 tcaatcagtg ggtttatgct attgccaaag tcgtgtacaa tgtaaacatc atcaatggta
    31621 taggttcccc tgatgcagtc caacgttact gtaacaactc ctgtcccata aacatggtag
    31681 gcaaaggggt gtcctttctc ctttgtttta tcaaagtaga taaccggagt ggcatagtga
    31741 ccgttctcag ttagcgcaac ccttttcagg tgtgcctttt taaccagctc gttccatttc
    31801 agggctgtgg gtgcaccgtt acggataacc gtttcgtttt caatgttcag ctcatcatcg
    31861 ctgcattcca gtagttcggc ggctacctta agcaaccgtt tttttagggc agcaatggca
    31921 attcgtagtg cattgccgtt caggtcggca ccgctactgg cagccgttgg tgaggtgttt
    31981 gcaacccttg ttgtattggt agaatgaatc tttacccggc taatatcaat ccccagcaga
    32041 atggatgcta cctgcgccat tttggtgttc acaccttgtc ccatttcaat ggctccagtg
    32101 cttactgaaa cgctgccatc ctggtaaata tgaacaaggg cgcgagcctg gttcattgct
    32161 gtgttggtaa acgatatgcc aaagcagagc ggcataaccg ccaagcccct tttgctgtac
    32221 ttgttgtttt ggttgtaatt atcaatggat ttacggagat tctcaaggtc aaacctttcc
    32281 gatgcaaggg caaagcactt acgggcgttt acgttttcgg ccttttgccc gtactggaat
    32341 tcatcgttct cttttagcag gtttgcctgt tggatttcaa ttgcgcttac accaatggtt
    32401 tcggctgctt tagcaatggc gctttcaatt acaaacattc cctgaggccc accaaaacca
    32461 cgaaacgcag tattgggcgg aaggttggtt ttgcagctat acacggttac tcgcgtgtta
    32521 gggatatagt aagaaccagt ggagtggaag agcgttcgct ccatgatagc tggcgagagg
    32581 tcggctgcgg cgccgccgtt ttggtacagg gttgtttcaa aggccaatat cttgaggtca
    32641 gcggttagcc caatcctgaa atcgccgctg taggggtgac gttttccggt cattcgcata
    32701 tcgtcgtgac ggggtagaac cagttttacc ggtttgccgg taatatgagc agcaagtgca
    32761 gccatggctg caaatcctga agcctgatcc tccttgcccc caaaaccacc accaagccta
    32821 accacatcaa cctcaataag gttcatgggt aatcctagaa cacgagcaac cgttttttga
    32881 acggctgtag gtccctgggt cgatgagtga accttaatat agccaccctc aacgggatag
    32941 gcgtaagccc cttgggtttc aatgtacagg tgttcctgtc cgcccaactc aatgctatcc
    33001 tccacaatgt atttacattg gctccatacc tcattggggt tacccatgct gaaggttcgg
    33061 ggtggtatga gcagtaaccc cttttccttt gcaatgcgtg ggtcggttac cggttccagt
    33121 tcctggtaat caatccgaac cattttggct gctttgtggg cgtcgcgctc ggtttttgca
    33181 ataaccacag ctatgggttg accctggtag tggacttcgc cctcggcaag cagggtttcg
    33241 tcctcaacta ttcctcctat ctggttttcg cccggaatat cggtgcaggt gatgactttt
    33301 accacgccat ccagccttag tgcctccgtg gtatcaacct ttaaaatttt accgtgggca
    33361 atggctgagc ctacaactgc tgcatgcaat gtacccgaaa tggttgggat atcgtcgagg
    33421 taaaccgatt ttcccgttac atgacctatg gtatcgatat ttttcatcac tgttagcctt
    33481 aaaaaatctg gtcaaactgt tttcccaaaa acctttgcag gtgtgccagg aaaagctgtt
    33541 ttagaagcaa cttcttatac tctgcggttc cacggatatc gctaatgggc gatatctcat
    33601 cgtgcataat tgttattgct tgggttacag attgtgaatt gatttcctta ccagaaagat
    33661 agtttgatgt ttggtgcaga tataaaggga ttggggcaac cccacctgcc gatatccggc
    33721 acttttttac ctttccgttc tcgacttcga taagcattgc actgtttacg cttgcaatat
    33781 caaggtattt tcgtttgctt accttttcaa agctgaattg agtagtgctc tttggcagct
    33841 caaaggtgat gtactgaatt acctcgccag cattcaggtt cagttgcttg tagcctttaa
    33901 agagctggtt taacggttgc tggcgcgttt ttccatcgga aaccgaggtt atttgagcat
    33961 ttaatgccaa tagcatcaca cttaaatcac caattggcga ggcgttcact atgtttcccg
    34021 caatggtagc catgttcctg atgggctccg acgaaaccag catcaggtac tccttaaggt
    34081 gagggaaacg ctccatcagc tctgctgaag caaccaggtc gctcacccgg gttgcaccgc
    34141 caacggtgca ggtgcaatcg tttaatgtaa tttccttggg gagatgtcca tccaaggtcg
    34201 attttacttt ttgttcgtag atactctcgg ggcgttgaac cataaggtcg gttcctccgg
    34261 ctatcagctg tgaggtgcta ctgctttcat catcaaaagc cttaatttgc ttaagtttac
    34321 ctggaacttc cagaaagtag ttgggaagat agcctttgcc aacaagccaa ccaacggggt
    34381 ttgatatctt cttttcctgc attacccttg caatatcggc tgcagcccgc tctatggatt
    34441 tgtatccggt acacctacat atgtttccgg ccactgcctc aatggcacgc tcagtagtag
    34501 gagtttcgtg gttcatgcaa aatgcggtga acgacattac aaatccggga gtgcaaaatc
    34561 cgcactgggt agcggcatta tcggccattg cctgctgaac cggactaagc gtattgctta
    34621 agttgatacc ctctatggtt acaacatgct taccgtgagc gttaattaat gggtacaaac
    34681 acgatgtaac gcttcggtat tcaactgtat cgtttgttag ttcgcctata agtactgtgc
    34741 aggcaccgca atcgccctcc ctgcacccaa tttttgtccc gggtaggttt gactcgtaac
    34801 ggataaaatc cagtaaactc attcccgggt tacatgaggt gtttacaggt ttgtcgttaa
    34861 gtataaaact tatcatggat gttattaata tgcttcacaa tatacaaatc aaccattaaa
    34921 aaaatcaagt tttatagtaa aaatggaata aaattgatgt atttttaagg aattaaattg
    34981 aaaaattctg gaaaccatga taaattttag gcttagcaat atcagtattg ctgttttgtt
    35041 tattgcacta ctttttactg ttggttgcaa ttcgaacaaa aacgatagct ttagggtgat
    35101 gagttttaac ataaggttcg ataa
//
LOCUS       DAOSAL010000019        33501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_950_length_33501_cov_48.122406, whole genome shotgun sequence.
ACCESSION   DAOSAL010000019 DAOSAL010000000
VERSION     DAOSAL010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 33501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33501
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_950_length_33501_cov_48.122406"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1434
                     /locus_tag="PK049_04390"
     CDS             <1..1434
                     /locus_tag="PK049_04390"
                     /inference="COORDINATES: protein motif:HMM:NF027726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusF/SusE family outer membrane protein"
                     /protein_id="HOK60831.1"
                     /translation="LAAAALAFTACDKDDDNDKPQILVEDGLYIVGEATAWADYDLKG
                     QMVGGINEVDQQPRSTMFEKYVALEAGKTFKIVEIAGKQAIEYGPDNIADVVTNGENE
                     QPNVTAKVGTYKTGGSYTVNESGLYHVVIDKELQKIAIIPVPYWGILGGSTPYGWNDA
                     NTKLQLKGSFNKQQMEFEITGLELRKGDFKLRYGSGWKFWLNDEGTVKANTNFGGAID
                     ALVPGGANIVLPVEKEGIYTVTMKWSAETGGLGMTASMTKTADVEPLPEYPETLFMIG
                     DGVGDWIWDNTDLPMIAVHSHPYMFWKIVWMNETGGFKFAPKKMWANDFGKSGDMSAD
                     SVYNKGSDNVPVPGTAGYYMVVVDLKNEKISISDVKVHLIGATIGNLWDAPVADGKFT
                     VDNANEVVTITKSLSAGELRMHVWHKWFPVQDPQPVAWWQAEFIILNNKIEFRGTGDD
                     QERVNLTAGNYKIDLNFKTGAGSITAQ"
     gene            1491..4280
                     /locus_tag="PK049_04395"
     CDS             1491..4280
                     /locus_tag="PK049_04395"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOK60832.1"
                     /translation="MKIFSTLLLIFVISTAYAQPITVTPALPTDADAVTIVFDATKAT
                     RSDLVGYTGEVYTHTGVKVEGNPDWQYVIGTWGNNTSQPKLTRTGTNTYQLTISPSIR
                     QFYGVPADKKITQMCFVFRSADATKQTEDIFYNVYEQGLSVSITNPSQTKPIYELNDN
                     ISIQVSANSSTSLKLYIDNAEVASTNQTAISFPYTADTYGKHWIKAEASDGTTTKYDS
                     VYILVRTPVDVATLPNGMRPGVNILNDNSVTIVLYDPPAKKNYTYLVGSFSDWLPDEQ
                     YYMKRTPDGKYHWLTLNGLEPNKEYAFQFLIDGYLRIADPFTNKTLDPNDQYIPSSVY
                     PNLMAYPTGKTTGIASVFSTNPTTYSWQTATFTPPTKENLVIYELHIRDFVGDSYIQT
                     VRDSLSYLKKLGVNAVELMPINEFEGNDSWGYNPSFYFATDKAYGKPNDYKEFIDEAH
                     SLGMAVIIDMVLNHSFGQSPLVQMYSTSDGSTLGTPTADNPWYNTTCPHPPYCWGYDF
                     NHESAETQKFVDSVLTYWLTEYKVDGFRFDFTKGFTNTPNAGSAYDATRIAILKRIAD
                     KIWSVNPNAYIILEHFCDNTEEKALAEYRSSEGKGMLIWGNMNYAYGEATMGWVSTSD
                     ISWVAAKQRGWSVPHVIGYMESHDEERLMYKNLQYGNASNSDHNVKTLPIALMRMQTA
                     ANLFIPFPGPKMIWQFGELGYDVSIDYNGRLGRKPLHWEYYQDDNRREIFNTFAHLNR
                     LKQQYEVFKTDNYDYNLSGAIKWLKLNSSAMDVVIAGNFDVNQKSTTITFNNTGWWYE
                     YYSGDSVQLSTTSYQLTLNPAEYRMFTSERIKRNDIYVGINQPKSSKAEIMVWPNPAS
                     GNIFIKYFQPSNSHVKVDMYNLTGQLVYSETRHNFSGENTIGINIPNELPRGLYILRI
                     SSNGFVNSSKVAIEH"
     gene            4475..5467
                     /locus_tag="PK049_04400"
     CDS             4475..5467
                     /locus_tag="PK049_04400"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HOK60833.1"
                     /translation="MGKVLAHSDTLKPLTDGTVKGFRFSVSQKTNGRKAWHNRHGMPY
                     VGIALTGIDLGNKRVLGNAWGIQPFISYQLLQFKKFSVRTDFGLGLAYITRKYSPSHN
                     PTNVAISTSINYWATANLTSSFKFSNQFNLTAGIETNHFSNGAIKKPNYGLNIVGLSF
                     GFTYNPERDKLDNCKKEVYLENSDSKSQLGIVILAGIKETGPAGGEKYYPLSISMEYL
                     MPLAQSYPLMAGLDIMFDKSTRFHIELSGKHYSPVNDDFQVGIKTGLQIPFNRLSLHG
                     HLGWYLYNKNPRLPLYYQKVGIRYRLSRTSQVQLELKTHLNIADHVGVGYAISI"
     gene            complement(5491..6315)
                     /locus_tag="PK049_04405"
     CDS             complement(5491..6315)
                     /locus_tag="PK049_04405"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOK60834.1"
                     /translation="MVCRAKIVGYKGLLLPNKVKAILFIFLVSWGVNAQEKPLVYPFR
                     VGEVVKYNAYYNWQFVWLNAGTAEFYINDTLVNGVPAYHFKSLGASLNSYDWFFKVRE
                     RFESIAKKENLAPLWFIRESYEGGYKVFNRYSFSYSNSKLVIDSYTSNRPYKQNTFTI
                     DKPIFDVLTAIYYCRTIDFKRLNKGDLVPLTMAVDDGVYNLYIRYLGPENIKHRNGNS
                     YDTYKFSVKLVEGTIFKGGEDMIVWVSRDEKKIPIVVEAKILIGSVMAYLLEYKVN"
     gene            complement(6305..7279)
                     /locus_tag="PK049_04410"
     CDS             complement(6305..7279)
                     /locus_tag="PK049_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HOK60835.1"
                     /translation="MIKRGIKDYILLVLKGIGMGAADVIPGVSGGTIAFITGIYEELI
                     DSIKSVVAPESLKYLKSFSLLSYLKAINADFLLAVIGGIAISFLSLAKLMQYLLTNHP
                     IFIWSFFFGLILASVWFVAKIIEKWNVSTYLFFITGIILGFAITTITPTETPNDLWFI
                     FICGVVAICAMILPGISGSFILLLMGKYIYMMSALSSFNFPIILVFLLGAAVGILAFS
                     NFLSWLLHHFHAATIAVLAGFMLGSLNKVWPWKVATQTFIDAQGIIRPLIEKNVLPST
                     YLIENKAEPYLLGAIGFAILGAIIIILFERFFSKPVTKEVDLREKLGL"
     gene            complement(7290..8060)
                     /locus_tag="PK049_04415"
     CDS             complement(7290..8060)
                     /locus_tag="PK049_04415"
                     /inference="COORDINATES: protein motif:HMM:NF014709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HOK60836.1"
                     /translation="MNYNLSFIPARTQKPREYGLTMVMDKGLSMSEAQSLIESSGQYV
                     DFVKLGFGTSLVTNNLKEKVKLYKNAGLRPYLGGTLFEIFIIRGMFDDFVKFVDELEL
                     DLVEVSDGSMELDNHVKCDYISKLAKRYTVISEVGSKRADVHIPDEEWINMMKHELSS
                     GSWKVIAEARESGNIGIYNQDHSANTKLIDDIVRHVKVENVIWEAPLKSQQAWFIKML
                     GANVNLGNIAPNEVISLETLRLGLRGDTFFQFLPDELK"
     gene            complement(8184..9584)
                     /locus_tag="PK049_04420"
     CDS             complement(8184..9584)
                     /locus_tag="PK049_04420"
                     /inference="COORDINATES: protein motif:HMM:NF020855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOK60837.1"
                     /translation="MRDESSDYLSFEELFNLVERFDESIQSGRAQFFDVHEFVELIDF
                     YLDHEDDEKALVALKHAENIYPGSIEIKYKWAEYYHFTGDNEKALELIHEVEKIEKFN
                     ADLLYVKGEVLFELGRLNAAVECFDKAVSADTDDNIELLHRISTYFLENEEINLALKY
                     LLISYNKEKNNLAVLFDMGYCFERLNELDKSVKYYNEYLDINPFSAPAWYNLGIVHTK
                     LGEFDKAIECYDFCIAVDPQYSSAFHNKGNTLASLEKYTDALKVFNELAELESENPRV
                     FALIGECYEKLEQFDKALEAYNKSIVIDPDFAEGYYGIGVVLAARKKYDLSLNFIRRA
                     ITLNPEEYDFWLGLGRVYFETNDLENSLKAYREATSLNPDLPDAYLSLAEVLLYEERF
                     TEVEQLIDGLGNKFDSNVSIKIINAAALYLSHRPKEALDILKDAKSIDPSSIDDFINL
                     VNPDADKEFIENINKL"
     gene            complement(9786..10772)
                     /locus_tag="PK049_04425"
     CDS             complement(9786..10772)
                     /locus_tag="PK049_04425"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HOK60838.1"
                     /translation="MANLSTSYMGLNLRNPLIVASSGLTENIDDIVEFEARGAGAVVI
                     KSIFEEEIIAKAHDNLNKMQASGFIYPETMEYFDYDSMEDPISNYLDFLRKVKSETSI
                     PIFASINCVTSEGWIDFAKRIEDTGVDGLELNVFSLPSDPDRSAEENERIYFEIADRV
                     TSVVKLPVAMKVGYFNGALANFLVRLSSTPIKALVLFNRFYNPDFDVNTLEFTPASVF
                     STPSEISTSLRWVAIMSGRVSCDIAASTGVHDGNAMVKQILAGANAVQACSTFYRNGK
                     EQASFIIRQLEEWMNSNGYKTIDEFRGKMSQKKTYNPAAFERVQFMKYFHDL"
     gene            complement(10903..11787)
                     /gene="dapA"
                     /locus_tag="PK049_04430"
     CDS             complement(10903..11787)
                     /gene="dapA"
                     /locus_tag="PK049_04430"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOK60839.1"
                     /translation="MDTAKLRGLGVAMVTPFSYNYQIDYPALDKLIEFLIGNGVNYLV
                     VQGTTAETATLSDSEKRQLADYVVKKVNGRVPIVLGIGGNDTHKVIESYTKFDLTGID
                     AILSVTPYYNKPTQKGLYLHFKTIAQNTHLPIIVYNVPGRTGVNMTAETTLKLAHEFQ
                     GKIVAVKEASGNLTQMGYILRDRPSNFLVLSGDDGLTLPQISMGADGVISVLGNCAPA
                     KFSRMVNLALQGNFTDAAELHLGLIELIDLLFVEGNPGGVKAALNALGLIENVLRLPL
                     APVSDSTYDKIKKEIEKL"
     gene            complement(11798..12355)
                     /locus_tag="PK049_04435"
     CDS             complement(11798..12355)
                     /locus_tag="PK049_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60840.1"
                     /translation="MFDNLKYNKSLKELGKLTKFLKRQRQMYNLTTARHVGIVFNLTD
                     SKVFDAVVGFKSMLERIPITVEAVGYFNDKEVPQLYTMEKGIKVFSRGELNWYKKPEA
                     TVVSEFVAKDFDILIDLTQDEVLPLRWVSSLSKAKFKVGALNYFNNPFDLIVTVDKSL
                     GLEYLTQQIYNTLEVLNNRFAQQTV"
     gene            complement(12423..14432)
                     /gene="ligA"
                     /locus_tag="PK049_04440"
     CDS             complement(12423..14432)
                     /gene="ligA"
                     /locus_tag="PK049_04440"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOK60841.1"
                     /translation="MDSQKALERIGFLRNELNRHNYLYYVKAQPEISDYDYDQLLREL
                     IDLEKQFPEFDDPNSPSRRVGSDISQEFVQVRHKYPMLSLANAYSYSELTDFDNRIRK
                     ALPEPFQYVCELKFDGVAIGLTYRNGRLVQAVTRGDGTMGDDVTVNVRTIRTIPLELH
                     GADFPEEFEIRGEIFMPRNVFDEINSEREDIGETPFANPRNAAAGTLKLLNSAEVARR
                     KLDCTLYYLLGEKLPADNHYENMLKAKEWGFKVSEHMTLCNNLTEVQHFIEHWDKARK
                     KLPFDTDGVVIKINSYRQQNILGLTAKTPRWAVAFKYKPERVSTELLSVDFQVGRTGA
                     VTPVANLKPVKLAGTTVKRASLHNADQIQLLDIRVGDWVFVEKGGEIIPKIVGVDQSK
                     RSLFSKPIDFPGACPECGTTLVRPEGEARHFCPNQYGCPPQIKGRIEHFISRKALNID
                     GLGEETVALLFDNNLIRDAADLYSLTREQLLALDRFADKSADNAIMSIEKSKSVPFPR
                     VLFGIGIRYVGETTAKKLAQHFGSIDAIATATVEQLVEVEDVGDRIALSIIEFFSDER
                     NRMLIDKLKAAGVQMAVSSADTSLLSDRLKGMSIVISGTFSRVSRDELKELIARHGGK
                     NVSSISASTSLLIAGENMGPAKLEKATKLGIKIIDEEEFFRIIEG"
     gene            14655..16832
                     /locus_tag="PK049_04445"
     CDS             14655..16832
                     /locus_tag="PK049_04445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HOK60842.1"
                     /translation="MKNNDLNFKAFELSKSSLMKSIQELQPDSKANQVVEALDMAKSI
                     YEKHGEWTDSIATHLVNVAQICLNEIGLGTTSSIAALLHETIERKWLTPVEIEKKFGK
                     AVASIVLNFTQISGLDPKDPKTQAELFRQMLISLSSDARVILIKLADRLDTMRSLSKE
                     QPDVRLKRSWETFHLYAPLAHRLGLYRLKSEMEDLAMKHIYPKEYKYIIKRLQETTAS
                     RNRFLHSFMAPIEEELQRRGFTFTIKGRTKSIYSIFRKMQNQEVDFDEVHDIFAIRII
                     LDTKPENEKSDCWQVYSIITDKYTPNTERLRDWISVPKSNGYESLHITVLGPENKWIE
                     VQIRTVRMDEIAEKGLAAHWKYKGIRQEQGIDEWVARVREILESPDTSPEDKVEQFQM
                     NLTSSDIYVFTPKGDIRKLPAKSTVLDFAFDIHTEVGSQCIGAIVNGKNTTIKQELQN
                     GDVVEILTSKKQKPKKDWLTFVITSKAKSRIKQQLRDIENSEIQEGKEILLRRLKNWK
                     IDDVDEALRKVNKHLKLKKPTDIFILIAQGKVDLTAIKTLLTAEEEKSAVKEDIPKPE
                     AEKAEVKESSDYLVIDEKLVNIEYKLAKCCNPIFGDDIFGFVTVNEGIKIHRTSCPNA
                     GQLNQRWPYRVVKARWKGSSKAGAFQTTIKVTGTDEIGILNRISEVISKDLRVNMRNL
                     NVSSRDGLFEAQIQLYVEDTKHLEMLLYRLGRVQGVHKAIRLK"
     gene            17089..17706
                     /locus_tag="PK049_04450"
     CDS             17089..17706
                     /locus_tag="PK049_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60843.1"
                     /translation="MRKIFLTAAIALYAINSLQAQLVQDLYRIYEPIYRSSETDSTAV
                     FTPFGIVKPGTNYGLSMGTGYSFLGNGIGMSGSYIAPSVTYSNNRLQVIAGVTLSHTN
                     MHGLRPDGANFAQPYSGANPYQAWAYTQYQFSNRFSVYAMGAVSQNQTLFSPFNSYMG
                     TFNSQQFGVGFNYRLGSKTTIGASFNFVNRNPNNLFYNPNSPFGW"
     gene            complement(17808..19880)
                     /locus_tag="PK049_04455"
     CDS             complement(17808..19880)
                     /locus_tag="PK049_04455"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOK60844.1"
                     /translation="MLVLLAVSCQEKADKNPLLSKFDTPYQTPPFDKIKHEHYMPALD
                     SAISVARHEIDAIVNNTEEPTFENTIEALDHSGKLLADVSNILFNLNEAETDSVLQQI
                     VIEASPKLTDFSNDINLNPELFKRVKAVWEKRDSLKLTPEQMMLVDKTYKGFVRNGAN
                     LSEADKEKYRAISRELSELTVKFNQNVLAETNSYKLHITNEADLAGLPQSLIDAAAYT
                     AKQKGLEGWVITLDYPMYGPFMKYADNRELRKQLYMAYGNRCFKGNEYDNQKIAQRIA
                     NLRLELANLLGYPNFATFVLENRMAETPERVNQFLDQLVAASLPAAREEVKEVEEFAR
                     QQGFKGKLERWDWSYYSEKLKNAKYSYNEEELKPYFQLEKVIDGVFLLANKLYGLTFL
                     PNGKIPVYHPDVKAYEVYDGSGRFMSVLYLDFFPREGKSGGAWMTSFRSQYRENGKDI
                     RPIVSIVTNFTKPTDKQPSLLTFYEFSTFLHEFGHALHGMLTDCNYSSLSGTSVYRDF
                     VELPSQIMENFAVEKEYLDLFAVHYQTGEKIPQELVQKIIDSRNFQAGYFSLRQLGFG
                     ILDMAWHTITKPVTEDVDSFEKKILNPLDVLPPVKGTNMSVTFGHIFEGGYAAGYYGY
                     KWAEVLDADAFELFKEKGIFNREVAQSFRENILSRGGSEHPMTLYKRFRGQEPTIDAL
                     LKRSGLKK"
     gene            complement(19986..20432)
                     /locus_tag="PK049_04460"
     CDS             complement(19986..20432)
                     /locus_tag="PK049_04460"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOK60845.1"
                     /translation="MNEFNVEILNLPENLYRAAIAIRGEITRRLTEAFGEEFTADYWF
                     IMNHLFKENPLTQGRLAELTNRDRASLSRTIACMERIDLVTKMAEPFDKRKELISPTK
                     TAINFKPEGERIVLQAVGMVMHNLKPIEVIELNRMLGQLFENITKG"
     gene            20537..23059
                     /locus_tag="PK049_04465"
     CDS             20537..23059
                     /locus_tag="PK049_04465"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HOK60846.1"
                     /translation="MNRKLALLSIFGLLLLLACKEKIESGVSQQLANQRKQTLSNIEY
                     NIYIDIPESENAPVPGRVELMFELNERYNLPIDFRNADSCLLNVTVNYRQISANVNNG
                     HVIIPKSYLYQGQNKIAIDFLAGKTSLNRKGDYLFSLLVPDRASTVFPCFDQPNLKAS
                     YNLTLQTPKDWVAVSNSPVINKMETDTSNLWIFDKTYPISTYLFAFTAGKFKAIDFVA
                     DSFKMTIYHRETDTQKVARNIKTVFELHQSAIKWLENYTRINYPFQKLDVVLIPDFPY
                     SGMEHQGAIFYRDSRILLDENSSVTQQLNQANLIAHEVSHQWFGNLVTMNWFNDVWLK
                     EVFAGLMADKIVNPQYPKLNHQLSFVLSHYPRAYSVDRTGGANPIRQNLDNLMLAGTL
                     YGDIIYHKAPIALQQLELMLGEDKFREGLNIYLKKYAYSNAGWNELIEIFDNLSSDDI
                     KSWSKRWIDAPGMPAIYVDIVSDVINISQTDNWLPMEFEVMIPTTAGYQTEKVRLNGK
                     HTEIPMPKNFTGLKAIIPNSNGLGYGLFVSADRDLTLLQLNELANNDVARASFFINLH
                     ELFLNHLVDTDTYFRFLANALSREKDPQIRNYLLGNLETVWWQFLDHTNQIILSQTLQ
                     EALQGIFNNPKIPADERKPAFLTYCRTSITPEAVEFIENIWAGRVQIKGISLSENDFI
                     DLSFQLAVRDVHDADSILQVQEERIQNADRLAKFRFVRRAATPDEALRDDFFHSLLIA
                     QNRRPEPWVAEGLRLFFHPLRANHSIHYLNAALDLLPEIQQTNDIFFPKTWLDAVLYG
                     HSSTEAAKIVTKWIQEHPQLSPNLKGKLMQSADLLFRAAGSK"
     gene            complement(23060..24214)
                     /locus_tag="PK049_04470"
     CDS             complement(23060..24214)
                     /locus_tag="PK049_04470"
                     /inference="COORDINATES: protein motif:HMM:NF012372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HOK60847.1"
                     /translation="MSSFRFIDLFAGVGGFHIAMSRLGGKCVFASEIDPSAAETYRIN
                     FGIDPLCDIREVDVSSIPQHDVLCAGFPCQAFSKAGKQQGFDDTRGTLFFEIKRILKH
                     HRPKYIVLENVRNLASHDGGRTWKVISENLKELGYIITEKPLIVSPHQVGVPQLRERV
                     FILGVHSSATKLRKLHFGIPPSHRNQTDGAIVLNGKADRRFAISNYEEYVLSAWDEFI
                     QGVGRLKLGFPIWANEFGKDYDLSNLPTWKAGFVQKNRMLYSQNKVFIDKWLKKYNYL
                     SEFVQTHTKFEWQAGEHIDTVWKGIIQFRPSGIRVKRPTEFPALVAMVHIPVVGWEKR
                     RLTPREVANLQSFPENYIINSNAQQAYKQFGNAVNVKVVEFLAKQLFMQA"
     gene            24386..25096
                     /locus_tag="PK049_04475"
     CDS             24386..25096
                     /locus_tag="PK049_04475"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOK60848.1"
                     /translation="MDNLIDSIRSDLKSQATEQNRIAGERFFKENVKLYGLKAAQVQT
                     IAKHYFSSQVKMMSKSEMLALSEQLWQSGFMEETFIACYFTERFVKHFSPEDFVTLEE
                     WVKKYINNWASCDTFCNHTIGDMLMLHPQLIEKVKLWAKSNNRWVRRAAAVSFIVPAR
                     KGLFHNHIVDIATMLLTDSDDMVQKGYGWMLKATSQSNQELVFNFVMSNKAKMPRTAL
                     RYAIEKMPLELKAKAMAK"
     gene            25195..26430
                     /locus_tag="PK049_04480"
     CDS             25195..26430
                     /locus_tag="PK049_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60849.1"
                     /translation="MKKSLFITAFSVFTAFISVAQEQTQKPKIEVKPYGYVAWETIFD
                     TYKSVDTRDGELYFYPLKANIDANGTDVNKKHQLQMLSLTTRLGLKISGPDVLGAKTS
                     AMVETDFFATATEYTRLLRVRHAIMTLKWQHSELIMGHYWHPIIVNEVIPSTLAFGAG
                     VPFHSLNRSPQIRFTYYPIEKVRLSATALTQGYHKSMGPAEAQRNSGLPELMAQAAFV
                     KNNSFIAGLSAGYKWLTPRLYITSSNTGTHKTVGQYLFSVFTAANLAKTNIKFETVYG
                     ENPTHLLMIGGYGRVTETTADSDYDYANLRTLSTWLDVDHKLGKWGIGVFAGYSKLLG
                     SNKNYSTIASYHRNDDMNYVYRISPRVSYKEENLVLGIEYMLTTAVYGKTFDAQHKVT
                     SSLDPVSNNRITISAKYSF"
     gene            26536..28476
                     /locus_tag="PK049_04485"
     CDS             26536..28476
                     /locus_tag="PK049_04485"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HOK60850.1"
                     /translation="MVRVKKALRIGLLLLVLIISLLFLSRNLIVKKIFSKTSARIKNR
                     YGLTVTASAVEVKGLNTLQLQNLLIVDTDTLLCSESFRIKLNPFHLVLLKINPKQIDI
                     SRATINIDNLIAQASKHTLQSSNERKNESSSNKRLYHLVKAFFGLSTATFNINNLHFT
                     YSDSAYTGKVTIEKWSYSNNNFTSTLTLNDGDRASRVTVTGTTSKRKNSLTATVTTEQ
                     PEAYIPFTTPFLGVKASFKKIFLDLNATHIEPGRIELNLVSSINSLLLDGKRIAQKPV
                     IIDSCGANMKIRAIPNSFVIDSLSTVSLNGFKTNLGFEYNPLPNRYVRFTLNTGENSW
                     QSLFDALPKGLFTNLWGIKVNGTFSYSLQVGVPLTNPDSLTIAPNLKSKGFYVLSYGN
                     TNLAMLADTFTHRAYIDNVYVRDIKVNPKSASYASLDQISPYLQWAVITSEDGGFYNH
                     RGFSLDGITYAMACNIREKRFARGGSTITQQLVKNVFLNQSKNIGRKAEEILITWITE
                     NSGVASKERMLEVYLNIIEWGPDVFGIREASQYYFGKKPSSLTLPEALFLAYIIPRPA
                     KFRYLFDTEGKLKPFVTESFMFVANKMLNRGNITQQDYNNLATNPLVTLTGPAKDLLK
                     KESSLENIDSEGNFTEQEKETN"
     gene            28546..29214
                     /locus_tag="PK049_04490"
     CDS             28546..29214
                     /locus_tag="PK049_04490"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOK60851.1"
                     /translation="MNTQVSKNIEAVAESIQWFGQAAVKIMNCGKVIYIDPYQIKGND
                     KADLILITHSHFDHFSPDDIKKIVATHTWILCPSDVADEAQKFGAAKVIAVQPGFNDE
                     WHGIEIKTVPMYNLTKTDKHPKSKNWVGYLLNLCHVWLYHTGDTERIPEMKSIKTDII
                     LLPLGQTYTMNSVDDAVAAVLDTGASVAIPIHFGMYEGTTADVDKFKELLKDKVEVIV
                     LPKS"
     gene            complement(29219..29917)
                     /locus_tag="PK049_04495"
     CDS             complement(29219..29917)
                     /locus_tag="PK049_04495"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOK60852.1"
                     /translation="MRTFIIPDIHGCSKTLRFLLEVKLSVCKTDRLFFLGDYIDRGPD
                     TPGVVEYLLNLIDEGFNITCLKGNHEQMMLDCIKGDYEMSLWLMNSGFATTHQYESIV
                     TAIETDYGFLPKRHLNFFTSLKPYVVVDNQYLLVHGGFNYELPNPFADEYAMLWNRPT
                     EAPSSFMPGYKIIHGHTPRALAKIVEEFKDTQRRLYDLDAGCVYKGLYIPGTGYLTAL
                     QLENWRLEFVECLD"
     gene            29922..30722
                     /locus_tag="PK049_04500"
     CDS             29922..30722
                     /locus_tag="PK049_04500"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOK60853.1"
                     /translation="MVYINLTRKINFAKFFVVMASGYIIKKLQGKVNQAIYGYNLIDQ
                     GDKIMVGLSGGKDSYALLDLLVNRRKVLPIGFELHACHVQATDMTYRADIEFMEQYCA
                     QNNVKFHLRQISVEYNPNDRKPACFICSWKRRKMLFTTTREAGCNKLALGHHLDDAIE
                     TLLLNMINHSSISSIPPKLSMFNGEVFLIRPLTTCLDKELEKYSQFMGFPSEKELCQF
                     DKDTHRNTVRELIQHMEQINRDARENIYRSMQNIFDEYIVPKPRKKGN"
     gene            complement(30723..32162)
                     /locus_tag="PK049_04505"
     CDS             complement(30723..32162)
                     /locus_tag="PK049_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOK60854.1"
                     /translation="MRPHVIIRYIGMVMLFNAAFIFVSFLISLYNKDAGQLPLLFSFL
                     ITLLWGVFPLVFVPKATDLSNKEGYLVVVLSWILSCFIGSLPLLLYGGEFTFVKALFE
                     SVSGYTTTGASILTNVEALPKGILFWRSSMHWVGGIGIVVFVLIILPSLGKAQMTLSK
                     MEISPLAQENFRYRTRKMLNVIMVVYLGLTVTQVILLRMFGMGLFDAVNHAFATVATG
                     GFSTKNNSVAYFNSVPIDIIIIVFMFLSGIHFGLLYSTITLKKNNIFNSPIVKYYFWI
                     TIIGVALVSINLHGKIYPTWGESIRYAAFQVVSLSTTTGFANADSSIWPPFSILIIIF
                     FTIQCACAGSTAGGMKLDRVVIFFQALKSQILKIQHPQAVIPVRIGKSVINDEIVNSV
                     VQFIVFYILVLFVSTLILTGLGYDSLTSLSATAACMGNAGPGFGLVGSLSNYSMLSDS
                     ALLVLSFDMLLGRLEIFGLLLLFMVKSWR"
     gene            complement(32159..32920)
                     /locus_tag="PK049_04510"
     CDS             complement(32159..32920)
                     /locus_tag="PK049_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60855.1"
                     /translation="MFKIRYKIITGFAILGIMLVISGLISIYELTKLGNQVNRLLMDN
                     YRSIDFSKQMNFSLSLQERAVMLSIQGEKEKANSLFGNAVSTFNDNLLKASNNLTIPG
                     EASTVDSIALAFSKFKAKAEGFINEPSPNLQKYLTDVNPALEEVRRNVEDLLTLNQQN
                     LNQTVSVLEKSPYRTILPGLIIVITSVIFTIIFNYMISYYLLSPIKRITKGIQNFTKY
                     RRQYDVTIETRDEMYVLNESVKDLILTKSIQRKTE"
     gene            33254..>33501
                     /locus_tag="PK049_04515"
     CDS             33254..>33501
                     /locus_tag="PK049_04515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOK60856.1"
                     /translation="MDEKELVQQVVQHNNHEAFSILVERYKKMVITTCMGFLGNFHDA
                     EDIAQDVFVELYESLPEFRNEAKLSTWLYRIAVNKSLN"
BASE COUNT        10411 a         7214 c         6501 g         9375 t
ORIGIN      
        1 ttagccgctg ctgcacttgc ttttacagca tgtgacaaag atgatgataa tgacaaaccc
       61 caaattcttg ttgaagatgg tctttacata gttggtgagg caactgcctg ggctgactat
      121 gatttaaaag gacaaatggt tggcggtatc aacgaagtag accaacaacc aagatctacc
      181 atgtttgaga agtatgtagc ccttgaagct ggtaaaactt ttaagattgt agaaattgct
      241 ggtaaacagg ctattgagta cggccccgat aatattgccg atgttgttac caatggcgaa
      301 aatgaacaac ccaatgttac tgcaaaagtt ggtacttata aaactggtgg tagctatact
      361 gttaatgaaa gtggtttgta tcatgttgtg attgacaagg aactgcaaaa aatagctatt
      421 atacccgtac cttattgggg cattttaggc ggatcaactc cgtacggttg gaacgatgct
      481 aatactaaac ttcaacttaa aggtagcttc aataaacaac aaatggagtt tgaaattact
      541 ggtcttgagt tacgtaaagg cgattttaag ttacgttatg gtagcggttg gaaattctgg
      601 ttaaatgacg aaggaactgt aaaagcaaac acaaactttg gtggagcaat tgatgcactt
      661 gtacctggcg gagcaaacat agtcctacca gttgaaaagg aaggtattta tactgtaacc
      721 atgaagtggt cggctgaaac tggtggttta ggtatgactg caagcatgac aaaaaccgct
      781 gatgttgaac ctctaccaga ataccctgaa acccttttca tgattggaga tggtgtaggc
      841 gattggatct gggataacac tgatctgcca atgatagcag tacatagcca tccttacatg
      901 ttctggaaaa tagtatggat gaatgaaacc ggtggattta agtttgctcc taagaaaatg
      961 tgggctaatg attttggtaa aagtggagat atgagtgcag actcagttta taacaaagga
     1021 agcgataatg ttcccgttcc tggaactgct ggctactaca tggtagttgt tgatttgaaa
     1081 aatgaaaaaa tttctatttc agatgtcaag gttcacctga ttggtgctac aattggtaat
     1141 ctttgggatg cacctgtagc cgatggtaaa tttactgttg ataatgccaa tgaggttgta
     1201 acaataacca aatccctttc agcaggtgaa cttagaatgc atgtatggca taaatggttc
     1261 cctgtacaag atccgcagcc tgttgcttgg tggcaagcag aatttatcat tctgaacaat
     1321 aagattgaat tccgtggtac aggtgacgat caggaaagag taaaccttac cgcaggtaat
     1381 tataagatcg atcttaactt caagacagga gcgggttcaa ttactgctca ataaataatt
     1441 attcactaaa agggcgcatt cattgcgccc tttttttaaa ttttcaaaca atgaaaatat
     1501 tttctacatt attattaatt tttgttattt caactgctta tgcccaaccc attaccgtta
     1561 cccctgctct ccctaccgat gccgatgcag ttacaattgt ttttgatgca acaaaggcaa
     1621 cacggagcga tctggtagga tatacaggcg aggtatacac ccataccggt gttaaggttg
     1681 aaggtaaccc tgactggcag tatgttattg gaacatgggg aaataatact tcgcagccta
     1741 agctaacccg tacagggact aacacttacc agctaaccat ttcgccaagc attagacagt
     1801 tttatggagt gcctgccgat aaaaagataa cccagatgtg ctttgtgttc cgaagtgccg
     1861 acgccacaaa gcagaccgag gatatcttct acaatgttta tgaacaagga ctttcggtta
     1921 gcataactaa cccctcgcaa acaaagccaa tatatgaact aaacgacaat atcagcatac
     1981 aggtttcggc aaatagttca acctcactaa agctttacat cgataatgct gaggttgcca
     2041 gtacaaatca aacagcaata tctttcccct acactgccga tacatacggt aagcattgga
     2101 ttaaagccga agcatcggat gggacaacca ccaaatacga ttcggtttat atcttggtac
     2161 gaacgcctgt agatgttgca accctaccaa atggcatgcg cccaggagta aacatcttga
     2221 acgataattc tgttaccata gttctatatg atcctcctgc aaaaaagaat tatacttacc
     2281 ttgtaggtag cttttccgat tggctgcccg atgagcagta ctacatgaaa cgcacgcctg
     2341 atggtaagta tcactggtta acactcaacg gacttgaacc aaataaggag tatgcattcc
     2401 agttcctaat tgatggatac ctacgtattg ctgacccgtt cactaacaaa actcttgacc
     2461 caaacgacca gtatataccc tcctcagtgt atcctaacct gatggcatac ccaaccggaa
     2521 aaacaacagg aattgcatcg gtattctcta ccaacccaac cacctatagc tggcaaacag
     2581 ctactttcac acctccaaca aaggaaaacc ttgttattta cgagttacat attcgcgatt
     2641 ttgtgggcga ttcctatatt caaacagtta gagattcgct cagctacctg aaaaagttag
     2701 gggttaatgc agtagaactt atgccaatta atgagtttga gggcaacgat agctggggat
     2761 ataatccatc cttttacttt gccaccgata aggcctacgg caaacccaat gattacaagg
     2821 aatttattga tgaagcccac agcctgggga tggctgtaat tattgatatg gtactaaacc
     2881 attcctttgg ccaatcacca ctagtacaaa tgtactctac ttccgatggt tcaaccctag
     2941 gcactccaac agccgataat ccctggtaca atactacatg ccctcacccg ccctactgtt
     3001 ggggatacga ttttaaccac gaaagtgccg aaactcaaaa gtttgtcgat tccgttctca
     3061 catactggct aaccgagtat aaagtggatg gattccgttt tgacttcaca aaagggttta
     3121 ccaatactcc caatgcagga tcggcatacg atgctacacg tattgcgatt ctgaaacgca
     3181 ttgccgacaa aatatggtca gttaacccca acgcttatat cattcttgaa catttctgtg
     3241 ataacaccga agaaaaggca cttgctgagt atcgtagcag cgaagggaaa ggtatgctaa
     3301 tatggggtaa catgaactac gcctacggcg aggctactat gggttgggtt tcaacatccg
     3361 atatatcgtg ggtagcagca aaacaacgtg gttggagcgt gcctcatgtt atagggtaca
     3421 tggaaagtca tgatgaagag cggctgatgt acaaaaacct tcagtacggc aacgcgagca
     3481 actcagatca taacgtgaaa acattgccaa ttgccttaat gcgaatgcaa accgcagcaa
     3541 acctatttat tccatttccc ggacctaaaa tgatttggca gtttggtgaa cttggttacg
     3601 atgtaagcat agactataat ggtcgccttg gccgaaaacc attacactgg gaatactatc
     3661 aggacgataa tcgcagagag atattcaaca cctttgccca cctaaacagg cttaagcaac
     3721 agtatgaggt ttttaaaaca gataactacg attataacct ctccggagca attaagtggc
     3781 taaagcttaa tagtagtgca atggatgtgg taattgctgg taattttgat gttaaccaga
     3841 agagtactac tatcaccttt aacaatacgg gttggtggta cgaatactac agtggcgatt
     3901 ccgttcaact atcaacaaca agctaccagt taacccttaa tccagcggaa taccgcatgt
     3961 ttacttctga aaggattaaa cgcaacgata tatacgttgg cataaatcaa cccaaatcaa
     4021 gcaaagcaga gataatggtt tggcctaatc ctgcctctgg aaatatcttt ataaaatact
     4081 tccaacccag caatagccat gtaaaggttg atatgtacaa cttaaccggt cagcttgttt
     4141 atagcgagac gaggcataac ttttcaggag aaaatacaat cggaattaat attccaaatg
     4201 aattacccag ggggctatac attctccgaa tatcgtcaaa tgggtttgta aactcatcaa
     4261 aagtagccat tgagcattag ttggtttagg ttttaggttt acatttttgg tgtaacctac
     4321 tcggattatt cgggtaggtt acttattttt ggatatgttt agagtaagat ttcactatat
     4381 caccctttta attctacatt gtttgggttt tgaattagat gctcagaacg ctgaacattg
     4441 ggcaaacatt tatagttcat ccgcgtcagg ttttatgggg aaagtattag cacacagcga
     4501 tacccttaag cctctaactg atggcactgt aaaaggattt cgattttcag tatcacagaa
     4561 aactaatggg agaaaagcat ggcacaaccg ccatggaatg ccttatgttg gaattgcttt
     4621 aaccggtatt gatttaggga ataaaagggt tctgggaaat gcctggggaa tacaaccctt
     4681 tatttcgtac caactacttc aatttaaaaa gttctcagtt agaaccgatt ttgggttagg
     4741 gttggcgtac ataacccgga aatactcccc ttcccataat ccaaccaatg tggcaatatc
     4801 aacctccatt aattactggg caacagcaaa tctgacatct agcttcaaat tctcaaatca
     4861 attcaacctt acagcaggca ttgagaccaa tcatttctca aacggagcaa taaaaaaacc
     4921 taactatgga ttaaacattg ttgggttatc gtttggcttt acctataacc ctgaaaggga
     4981 taaactagat aattgtaaaa aagaagtcta ccttgagaat tcagacagta aatcgcaact
     5041 aggaatagta attttagcgg gaatcaagga gactggtcct gcaggcgggg aaaaatacta
     5101 ccccttatca atatctatgg aatacctaat gccactagcc caaagctacc ctttgatggc
     5161 tggattagat attatgttcg acaaatcaac acggtttcac atcgagctaa gtggaaagca
     5221 ttatagccct gttaatgacg attttcaggt gggtataaaa acaggactgc aaattccttt
     5281 caaccgatta tcgctacatg ggcatttggg atggtacctt tacaacaaaa acccacgttt
     5341 accgctatac taccagaagg taggaatccg ctaccggctt tcaagaacct cacaagtaca
     5401 gctagagcta aaaacccacc tcaacatagc tgaccatgtt ggggtgggtt atgcaatatc
     5461 tatataacat aaaaaatgcc aaaaagcatg tcaattaacc ttatattcaa gtagatatgc
     5521 cataacagac ccaatcaaaa tttttgcttc aactacaatg ggaatctttt tttcatcgcg
     5581 cgaaacccat acaatcatat cttcaccacc cttgaaaata gtaccctcaa ccagtttaac
     5641 actaaactta taggtgtcat atgaattgcc attgcggtgc tttatgtttt ctgggcccag
     5701 gtaacgaata tacaagttgt aaactccatc atcaacagcc attgtcaacg gaactaaatc
     5761 gcccttattc aatcttttaa aatcgatagt tctacagtaa taaatggcag ttagaacatc
     5821 gaaaatgggt ttatcaatgg taaaagtatt ctgtttatat gggcggttgg aagtatagct
     5881 atcaataacc agcttagagt tgctatagct aaagctatac cggttgaaaa ctttgtagcc
     5941 accctcatag ctttcgcgta taaaccataa tggtgcaagg ttctcctttt ttgctattga
     6001 ttcaaacctt tcccgtacct taaaaaacca gtcgtaacta ttaagcgaag ctcctagcga
     6061 tttgaaatgg taagccggta ctccattaac caatgtgtcg tttatgtaaa attctgcagt
     6121 ccctgcattt agccatacaa actgccagtt gtagtaggcg ttgtatttaa ctacctcacc
     6181 cacacggaac ggatacacca agggtttttc ctgagcattt acaccccatg aaacgagaaa
     6241 aatgaaaagg attgctttta ccttatttgg taacagcaat cccttataac caacaatttt
     6301 agctctacaa accaagtttc tctcttaaat caacctcctt agtaacaggt ttagaaaaga
     6361 aacgttcaaa tagaataata attattgcac caagtatggc aaaccctata gcaccaagaa
     6421 ggtatggctc ggccttattt tcaataagat aggtagatgg taaaacattt ttctctatca
     6481 gtggacgaat tataccctga gcatcaataa aggtttgggt ggccactttc catggccata
     6541 ctttatttag tgaaccaagc ataaaaccag caagaacagc aatagtagca gcatggaaat
     6601 ggtggagtaa ccacgataaa aagtttgaaa aggccaaaat gccaactgca gcacccaaca
     6661 aaaaaaccaa aattattgga aaattaaaac tgctgagtgc cgacatcatg tagatatact
     6721 tacccataag aagaagaata aaacttccgg aaattccagg taagatcatt gcacaaatag
     6781 ccacgacacc gcaaataaaa ataaaccaca aatcattagg agtttcggtt ggggttatgg
     6841 tagtaatggc aaagccaaga ataatcccag taataaaaaa caagtatgtt gaaacattcc
     6901 atttctcaat aatcttggcc acaaaccaaa ccgatgccaa aatgagcccg aaaaagaacg
     6961 accatataaa aatgggatga ttggtcaata ggtactgcat caacttggcc agtgagagaa
     7021 aactaatagc aataccacca attacagcca aaagaaaatc ggcgttaatg gcttttaaat
     7081 agctaagtag tgagaatgac ttaaggtact ttaatgattc aggcgcaact acgcttttaa
     7141 ttgaatcgat aagttcttcg taaatacctg taataaaggc tattgtacca cccgacactc
     7201 cagggattac atcggcagcc cccattccaa tcccctttaa aactaaaagg atgtaatcct
     7261 ttattcctcg ctttatcata ttaaatcaac tattttagct cgtcaggtag aaactggaag
     7321 aatgtatcgc cgcgtaagcc gagccttagg gtttccagag aaataacttc gtttggagct
     7381 atattcccaa ggttaacgtt agcgcctagc atctttatga accatgcctg ttggctttta
     7441 agtggggctt cccaaattac attttcaacc tttacatgcc taacaatatc gtcaattagc
     7501 ttagtgttgg ctgaatggtc ctggttgtaa atgccaatat ttccactttc gcgtgcctcg
     7561 gcaataacct tccagctgcc ggacgatagc tcatgcttca tcatgtttat ccactcctcg
     7621 tcaggaatgt gaacatcggc acgctttgat cctacctcag aaataacagt ataccgtttg
     7681 gctaacttgc tgatataatc gcacttaaca tgattatcaa gttccattga gccatcggaa
     7741 acctcaacca ggtcgagctc taactcatcg acaaacttta caaagtcgtc gaacataccc
     7801 ctaatgataa agatttcgaa tagggtacct ccaaggtaag gccgaaggcc tgcattctta
     7861 taaagcttta ctttctcctt caaattatta gttaccagcg atgttccaaa acctaacttc
     7921 acaaaatcga catattgacc gctactttca atcaggcttt gtgcctcgga catacttaaa
     7981 cccttatcca ttaccatggt taacccgtat tcacggggct tttgggttcg agctggaatg
     8041 aacgataagt tataattcat agcaaaaaat tttttgttta aagccaaatg tatgattttt
     8101 aaaataaaaa agctgcattt acatgcagca aattattaac aatgcttttt ctacggaaaa
     8161 gttaacttcc gtaatggtat gatttaaagt ttattgatat tttcaatgaa ttccttatcg
     8221 gcgtcaggat taaccaggtt tataaagtcg tcgattgatg aggggtcgat actttttgca
     8281 tccttgagaa tatcaagagc ttccttgggt ctatgcgata ggtacagggc agccgcattg
     8341 attattttga ttgagacatt tgagtcaaac ttattgccca ggccatcaat gagctgctca
     8401 acttccgtga atcgttcctc gtatagtaaa acctcggcaa gtgataggta tgcatcgggc
     8461 aaatcggggt ttaggcttgt agcctcacgg taagccttta gggagttctc caaatcgttt
     8521 gtttcaaagt aaaccctacc caatcccagc caaaaatcgt actcttcagg gtttaaagtg
     8581 atagccctcc taatgaaatt gaggctaagg tcatactttt tacgtgctgc aagcacaacc
     8641 cctatgccat aatacccctc agcaaaatca ggatcaatca ctatagattt attgtaggcc
     8701 tcaagggctt tgtcgaactg ctcaagtttc tcgtaacact cgccaattag cgcaaacacc
     8761 cttgggtttt cggactcaag ttctgctaac tcattaaata cctttaaagc atcggtatat
     8821 ttttccagcg aggcaagtgt attaccctta ttatgaaacg ctgaagagta ctgtgggtca
     8881 acggcaatac aaaaatcgta acactcaatg gccttatcaa attcaccgag cttggtatga
     8941 actattccta agttgtacca agcaggagct gaaaatgggt taatatctag gtactcatta
     9001 tagtacttga cacttttatc gagctcattt aatcgctcaa aacagtaacc catgtcaaaa
     9061 agaacggcta agttgttctt ctccttgttg tatgatatca ataagtattt cagcgcaaga
     9121 tttatttcct cgttttccag gaaataggtt gaaatgcgat gtagtagctc aatattatcg
     9181 tcagtgtcag ctgaaacagc tttatcgaaa cactccacgg cagcatttaa ccttccgagt
     9241 tcaaaaagca cttcgccttt tacgtataac aggtctgcat taaatttttc aattttttct
     9301 acctcatgaa tgagctcaag ggctttttca ttgtctccag taaaatggta gtactccgcc
     9361 cacttgtatt taatttcaat tgaaccaggg taaatattct cagcatgttt taatgcgacc
     9421 agtgcctttt catcatcctc atgatctaag taaaaatcaa taagttcaac aaactcatga
     9481 acatcgaaaa actgagcccg cccgctttga atgctttcat caaaacgttc aaccaaatta
     9541 aatagttcct caaaactcag ataatcgctg ctctcgtccc tcatgcttta aaaataatta
     9601 acctacaaac atactaataa aatgaatgtc acccttagta aagttagtta aattaagtgt
     9661 aaatacctaa aataatcccc aaaaaagggt aataacttat taacatcaaa tgtatcttat
     9721 gaacacggtt aattaaacca tgcaaaatgc aatagataat ggtgaatcaa agattaaaag
     9781 gggaactaaa gatcatgaaa atacttcatg aattgtaccc tttcaaaagc agcaggattg
     9841 tatgtttttt tctggctcat cttgccacgg aattcgtcaa tagttttata tccattagaa
     9901 ttcatccact cctcaagctg gcgaatgata aatgaggctt gttccttgcc gttacggtaa
     9961 aaggttgaac atgcctgaac ggcattagca ccagccagaa tttgcttgac cattgcattg
    10021 ccatcgtgca caccggttga tgcagcaata tcgcagctaa ccctaccgga cataatagct
    10081 acccaacgga gcgaggtgga aatctctgaa ggagtgctaa aaacactcgc cggagtgaac
    10141 tccaaggtgt ttacatcaaa gtcgggatta tagaacctgt taaacagaac caatgctttt
    10201 attggggtac tactcaacct cacaaggaag tttgctagcg ccccattgaa gtaaccaact
    10261 ttcatggcta ccggtagctt aactacactg gtaacacgat ctgcaatttc aaagtaaata
    10321 cgctcattct cttcggctga acggtcaggg tcagaaggta atgagaagac attcaactca
    10381 agtccgtcta ccccagtatc ctcaatgcgt ttggcaaaat cgatccaccc ctcggatgta
    10441 acacaattga tgctggcaaa aatgggtata gatgtttcac ttttaacctt tctcaggaaa
    10501 tcaagatagt tagatatggg gtcttccatg ctgtcataat caaaatactc catggtttcg
    10561 gggtaaatga aacctgatgc ctgcatcttg ttaagattat catgggcttt ggcaataatc
    10621 tcctcctcaa aaattgattt aattacaaca gctccagcac cacgcgcttc aaactcaacg
    10681 atatcatcga tattttcagt taaacctgaa cttgcaacta ttaatggatt gcgcaggttt
    10741 aaacccatgt aggatgttga tagatttgcc atagccgaaa gatttaatta gtaattgact
    10801 aacaataaat tttttaaaga gttcactaaa tgtaaggcaa attaattaat aaagcaaaaa
    10861 gggaagcatg tggcttccct tttattcaat cagaaaggtt gattatagct tttcaatttc
    10921 ctttttaatc ttatcgtagg ttgaatcgct tacaggcgca aggggcagac gaagaacatt
    10981 ttcaattagg cccagtgcat taagagcagc cttcactcca ccagggttac cctcaacaaa
    11041 aagtaaatcg ataagctcaa tcaaacccag gtgtaattca gctgcatcgg taaagttgcc
    11101 ctgaagggca aggttaacca tccgtgagaa cttagctgga gcgcagtttc ctaaaactga
    11161 gattacgcca tcggctccca ttgatatttg cggtagcgtt aaaccgtcgt cgcccgaaag
    11221 gactaggaag ttggaaggcc tatcgcggag aatataaccc atttgggtta ggttgcccga
    11281 tgcttcctta accgcaacaa ttttaccctg gaactcatgg gcaagcttaa gggtggtttc
    11341 ggctgtcata ttcacaccag ttctaccggg aacgttgtac actataatgg gtaagtgggt
    11401 attttgagca atggttttaa agtgaaggta taatcccttc tgcgtgggtt tattgtagta
    11461 tggtgttact gaaagaatag catcgattcc ggttaaatca aactttgtgt atgattcaat
    11521 tactttatgg gtatcgttac caccaatacc taaaacaatg ggtacacggc catttacctt
    11581 tttaacaacg taatcggcca actggcgctt ttcagaatcg gaaagggttg ccgtttcggc
    11641 agttgttccc tgaactacaa ggtagttaac cccattgcca attaaaaact caataagttt
    11701 atcgagtgca gggtaatcaa tttgatagtt gtaactgaaa ggggtaacca ttgccacccc
    11761 aagacctctg agtttagcgg tgtccataaa aatattttta aactgtttgt tgggcaaaac
    11821 ggttgtttag aacctcaagg gtgttataaa tctgttgagt gaggtactct aaacccaacg
    11881 atttatcgac tgttactatc aggtcaaaag ggttattgaa gtaatttaaa gcaccaacct
    11941 tgaacttagc tttggaaagc gatgaaaccc acctgagcgg caaaacctca tcctgggtta
    12001 aatcaattag aatatcaaag tcttttgcta caaactccga tacaaccgtt gcttcgggtt
    12061 tcttatacca attaagttca ccccttgaga aaacctttat tcccttttcc atggtataaa
    12121 gctgtggtac ctccttatcg ttaaagtaac caacagcttc cacggtaatg ggaatgcgtt
    12181 caagcatgga cttaaatcca actactgcat caaaaacctt agaatcggta aggttgaaaa
    12241 ctataccaac atggcgggca gtagttaaat tatacatctg tcgttggcgt ttcaggaatt
    12301 tagttagctt accaagctcc tttaacgact tattatactt gagattatcg aacatctatc
    12361 taggtttatg ttgaaacgca attttacaaa aaaaacggtg taaatcaaaa aaagcttata
    12421 gtctacccct caataatcct aaaaaattct tcctcatcaa taatttttat tcccagcttt
    12481 gtggccttct cgagctttgc cggtcccata ttttcacctg cgataagaag cgatgtggag
    12541 gcggaaatgg aactaacatt tttaccgcca tgcctagcaa ttagctcctt aagctcatcg
    12601 cgcgaaactc gggaaaatgt gcctgaaatc acaattgaca ttcccttaag cctatcggac
    12661 aataggctag tatcagcaga gctaacagcc atttgaacac ctgctgcctt tagcttatcg
    12721 atgagcattc ggttgcgttc atcgctgaaa aactcgataa tactcaaagc aatacggtcg
    12781 ccaacatcct caacttcaac aagttgttca actgttgcag tagcaatagc atcgatactt
    12841 ccaaagtgtt gcgcaagctt ttttgcggtg gtttcaccaa cgtatcgtat tccaatgcca
    12901 aaaagaactc gcggaaaagg aactgacttt gatttttcaa tgctcataat tgcattatcg
    12961 gccgatttat cggcaaacct atctaatgct aaaagctgtt cgcgggtaag cgagtatagg
    13021 tcggcagcat cgcgaataag gttgttatca aagagcaagg caaccgtttc ctcacctaaa
    13081 ccatcgatat tcaatgcttt tcggctgata aagtgttcaa tcctaccctt tatttgcggt
    13141 ggacaaccat actggttcgg gcagaaatgg cgggcttcac cctcaggcct aaccaatgta
    13201 gttccacatt caggacatgc acccggaaaa tcgattggtt tactgaaaag tgaccttttc
    13261 gattggtcaa caccaacaat ttttggaatt atttcaccac ccttttcaac aaaaacccaa
    13321 tcgccaactc taatatcaag cagttgaatt tgatcggcat tatggagcga ggcgcgctta
    13381 acggtggttc cagctagctt taccggcttt aggttggcaa cgggcgttac agcaccagtt
    13441 cgtccaacct gaaaatcgac cgaaagcaat tcggttgaaa ccctttctgg cttatacttg
    13501 aaagcaacag cccaccttgg tgtttttgca gtaaggccca gtatgttttg ctgccgatag
    13561 ctgttgatct taataacaac cccatcggta tcaaagggca atttcttccg tgccttgtcc
    13621 cagtgctcaa tgaaatgctg aacttctgtt aaattattgc atagggtcat atgctccgaa
    13681 accttaaagc cccattcctt agccttaagc atattctcgt aatggttgtc ggcaggaagt
    13741 ttctcgccca ataggtaata aagagtacaa tcgagcttac ggcgagccac ctcagcagag
    13801 ttaagtaact tcaaggtgcc ggcagcagca tttcgtgggt tggcaaaggg tgtttcacca
    13861 atatcctcgc gttcactgtt aatttcatca aaaacatttc ggggcataaa tatttcgccc
    13921 ctaatttcga actcctcggg aaaatcggcc ccatgtaact caaggggaat ggttctaatg
    13981 gttcgaacat taacggtaac atcgtcgccc atagttccat cgccacgagt aacggcctga
    14041 actaatcgcc cattcctata ggtaagacca atggcaaccc catcgaactt tagttcgcaa
    14101 acgtactgaa aaggctccgg taatgcctta cggatacggt tatcgaaatc agtcagttca
    14161 gagtatgaat atgcgttggc caatgatagc attggatatt tgtgccttac ctgaacgaac
    14221 tcctgagaga tatcgctacc caccctccgc gatggagaat tgggatcgtc aaattcaggg
    14281 aattgctttt ctaaatcaat tagttcgcgc aggagctgat cgtaatcgta atcgctaatt
    14341 tcaggctgcg cctttacata gtaaaggtaa ttatgcctat tcaactcatt tcgcaaaaat
    14401 ccgattcgct ccaaagcctt ttgactatcc attttaaata gtttatgaat tcaaatttag
    14461 gctgaaaatt gattgaatga attaggacaa tatctgagtt attaacaaaa ttgatttatg
    14521 caataaaaac ttagaaccag ccatccttta tcctttatcc tttatccttt accctttacc
    14581 ctttaccctt tcaatatccc cttgcaatag attaactaaa tatcgtattt ttatggaaaa
    14641 gcaaactagg gcaaatgaaa aacaatgatt tgaatttcaa ggcattcgaa ttatccaaga
    14701 gttcgttaat gaagagcata caggaacttc aacccgacag taaggctaat caggttgttg
    14761 aggctcttga tatggcaaaa tcgatttatg aaaaacatgg tgagtggacc gattccattg
    14821 ccacacatct tgttaacgtt gcccaaatct gcttgaatga gattggactt ggtactacta
    14881 gctcaattgc cgcgttgtta cacgaaacta ttgaacgcaa atggttaacc ccggttgaga
    14941 ttgagaaaaa gtttggaaaa gcggttgcct caatagtgtt gaattttact caaatttcag
    15001 ggctcgaccc aaaagatcca aaaacacagg cagagctttt caggcaaatg ctaatcagcc
    15061 tatcgtccga tgcccgggtg atactgataa agttagctga caggctcgat accatgcgaa
    15121 gccttagcaa ggagcagccc gatgtcaggc taaaacgttc atgggaaaca tttcacctct
    15181 atgccccact tgcacaccgc cttggcttat acaggctaaa gtccgaaatg gaggatttag
    15241 ccatgaagca catctacccc aaggagtaca agtacattat aaaaagattg caagaaacca
    15301 cagccagtcg gaacagattc ctacatagct ttatggctcc tattgaggaa gaactccaga
    15361 ggagggggtt tacattcacc ataaagggaa gaaccaaatc catatactcc attttcagaa
    15421 agatgcaaaa tcaggaggtt gattttgatg aggttcacga catttttgcc atcaggatta
    15481 tacttgatac caaacccgaa aacgaaaaat ccgattgctg gcaggtttac tccattatta
    15541 ccgataagta cacacccaat acggaacgct tacgcgattg gatttcggtc ccaaaaagta
    15601 atggttacga aagccttcat atcacagtgc ttggacccga aaacaagtgg attgaggttc
    15661 aaatccgtac tgtacgaatg gacgaaatag ccgaaaaagg tcttgcggca cactggaaat
    15721 ataaaggaat aaggcaggaa cagggaattg acgaatgggt tgcccgcgtt agggaaatac
    15781 tggaatcgcc cgatacttcg ccagaggata aggttgagca attccagatg aaccttacca
    15841 gcagcgatat ctatgttttt acacctaagg gtgatatacg aaaactacct gcaaaatcga
    15901 cggtgcttga ctttgccttc gatattcata ccgaagttgg ctcgcagtgt attggagcca
    15961 ttgtaaacgg taagaatacc actatcaagc aggaacttca aaacggcgat gtggttgaaa
    16021 tccttacctc aaagaagcaa aagcccaaaa aggactggct aacatttgtc atcacctcaa
    16081 aagctaaaag taggataaag cagcagctcc gcgatattga gaatagtgaa atacaggaag
    16141 ggaaagaaat actgctacgt aggctaaaga attggaaaat tgatgatgtt gatgaggctt
    16201 tacgaaaggt aaacaaacat cttaagctaa agaaacctac cgatatcttt atactcattg
    16261 cccaaggcaa agttgaccta acggccatta agaccctact aactgccgag gaggaaaaat
    16321 ctgccgttaa ggaagatatc ccaaagcccg aagctgaaaa ggctgaggta aaggaaagtt
    16381 ccgattacct tgtaattgac gaaaaactgg tgaacattga gtataaactt gccaaatgct
    16441 gcaacccaat atttggtgac gatatttttg gctttgtgac agtaaacgag ggaataaaga
    16501 ttcaccgaac gtcctgccca aatgcgggtc agctaaacca gcgttggcca taccgtgtag
    16561 taaaagcaag gtggaaaggc tcgtcaaaag caggtgcatt tcaaacaacc attaaggtta
    16621 ccggaaccga tgaaattgga attttgaacc gtatatcgga agttatcagt aaggatttac
    16681 gggtaaacat gcgtaacctg aatgttagct cccgcgatgg tctgtttgaa gctcaaatac
    16741 aactatacgt agaggacacc aagcatcttg aaatgctgct ctatcgtctt ggcagagtgc
    16801 aaggtgtaca caaagcaatt agattgaaat gatttgcacg taaccattaa ctaaaaaagt
    16861 accacagagt tacactgagt gcaacagcgt tgcaaagcgt caaacccttt gtattcacta
    16921 tcaaccatca acctattctt taacctttat cctttatcct ttatccttta tcctttatcc
    16981 tttaaccttt atcccttttt cacccttccc ctaaactttt tttaaccaac cccatttacg
    17041 gttttcgcat tttttgcatt atattggtgg caggaaattt ttaaagttat gagaaagatt
    17101 tttttaacag cagcaatagc attatatgct ataaattctt tgcaggcaca gcttgttcag
    17161 gatttataca gaatttacga gccgatttat agaagttctg agaccgactc aactgctgtg
    17221 tttaccccat ttggtatagt taagcccgga acaaattacg gtttaagcat gggaacaggc
    17281 tattcctttt tgggcaacgg gattggcatg tctgggagct atatagcccc aagtgtaacc
    17341 tactcaaata accgcttaca ggtgattgct ggcgttactc tctcacacac aaatatgcat
    17401 ggattaagac ccgatggtgc caattttgct caaccttata gcggtgcaaa tccatatcag
    17461 gcttgggcat acactcaata ccaattctca aaccgtttta gcgtgtatgc catgggagcg
    17521 gtatcacaaa accaaacctt attctcaccc tttaatagct atatgggaac ttttaatagc
    17581 caacaatttg gcgtaggatt taattaccgt ttaggtagta aaacaacaat aggagctagc
    17641 tttaactttg ttaaccgcaa tccgaacaat ctcttctata acccaaattc tccttttggt
    17701 tggtaaagag aaactcaaat ttttggaata ctcaaaaatt tttcatcgaa acatattgta
    17761 aaaaaggctg ctcgctgagc agccttttta tattaacccc aatatcgcta tttctttaat
    17821 ccgctacgtt tcagtaacgc atcgattgtt ggctcctgac cacggaaacg cttgtaaagg
    17881 gtcatcggat gttcgctgcc acctctcgat aaaatattct ctctgaagct ctgagcaacc
    17941 tcacggttaa atattccctt ttccttaaaa agttcaaaag catcggcatc gagcacttcg
    18001 gcccacttgt agccatagta tccggcggca tatccaccct caaagatatg gccaaaggtt
    18061 acgctcatat ttgtgccttt taccgggggt aaaacatcca ggggattaag tatctttttc
    18121 tcaaagctgt caacatcttc agttacaggc ttagtaatgg tgtgccaagc catatcgagt
    18181 attccaaaac ctaactgacg taacgaaaag tagcctgcct gaaaattacg gctatcaata
    18241 atcttttgaa caagctcctg cgggattttc tcgccggttt ggtagtgtac cgcaaataag
    18301 tcgaggtatt ccttttcaac tgcaaagttt tccatgattt gtgatggcaa ctccacaaaa
    18361 tccctataaa ccgaggttcc gctaagcgat gagtagttgc aatcggttag cataccatgc
    18421 agtgcatgac caaactcatg aaggaaggta gaaaactcat agaaagtaag caacgatggt
    18481 tgtttatcgg ttggtttagt aaaattggta acaattgaaa caattgggcg aatgtccttg
    18541 ccattttctc ggtactgtga acggaatgag gtcatccatg cacctccact cttaccctcg
    18601 cgagggaaaa agtcgaggta taggactgac atgaaacgtc cgctaccatc gtacacctcg
    18661 tatgctttta catcggggtg gtaaacagga attttaccat taggtaaaaa ggtaagtccg
    18721 tacagcttat tggccagaag gaaaacacca tcgataacct tctcgagctg gaagtaaggc
    18781 tttagctcct cctcgttata gctatacttt gcattcttaa gtttttcgga ataatacgac
    18841 caatcccaac gttcaagttt gcccttaaat ccctgctggc gggcaaattc ctcaacctct
    18901 ttaacctcct cgcgagcagc aggcaacgat gcggcaacca gctggtcaag gaattgattt
    18961 actcgttcag gagtttcggc catgcggttc tcaaggacaa aggtggcgaa gtttggataa
    19021 cctagaaggt ttgccaattc taacctaaga tttgcgatac gttgagcaat tttttggtta
    19081 tcgtactcat tgcccttgaa acaacgatta ccgtaagcca tgtagagctg tttacggagt
    19141 tcacggttat cggcatactt cataaatggg ccatacatgg ggtagtcaag tgtaataacc
    19201 caaccctcaa gccctttctg tttagctgta taggcagcag catcaataag gctttggggt
    19261 aagcccgcca aatcggcctc attggttata tgtagcttat agctgttagt ttcagccaaa
    19321 acgttttggt taaactttac tgttaactcg gaaagctcgc ggctaatggc acgatacttc
    19381 tccttatcgg cctcgcttag gttagcccca ttacgcacaa aacccttgta ggttttatca
    19441 acaagcatca tttgttcagg agtgagtttc agggaatcgc gcttttccca aaccgcctta
    19501 acccttttaa agagctcagg gttaaggttg atgtcgttgg agaaatcggt aagctttggt
    19561 gaggcctcaa tcactatttg ctgaagaacg ctatcggttt cagcctcatt taaattgaat
    19621 agaatattgg aaacatcggc aagcagctta ccactatggt caagggcctc aatggtattc
    19681 tcaaaggttg gttcttcggt attattaact attgcatcaa tctcatgacg agccaccgat
    19741 atagccgaat caagcgccgg catgtagtgc tcatgcttta tcttatcgaa cggtggtgtc
    19801 tgatacggtg tatcaaactt acttagtaat gggttcttat cggctttttc ctgacacgat
    19861 actgcaagta atactaacat aatgcccaga attgattttg attgttttag cataataaat
    19921 tattctttta aaatttggtg tcaaaagtaa ttctttttgt acagatagat cttgatttcg
    19981 agaatttaac ccttggtgat gttttcgaac agttgaccaa gcattcggtt taactcaata
    20041 acctcaattg gcttaaggtt atgcatgacc atccctactg cttgtaagac tatcctctcc
    20101 ccctcaggtt taaagttgat tgcagttttt gtaggtgaaa ttaactcctt tcgtttatcg
    20161 aacggctctg ccattttagt tactaagtca atgcgctcca tgcaggctat tgttcgggaa
    20221 agtgaagccc tatcgcgatt ggttagctca gctaaacgac cttgtgttaa aggattctcc
    20281 ttaaaaagat gattcataat gaaccagtaa tcggcagtaa actcctcgcc aaacgcctcg
    20341 gttaaccttc ttgtaatttc cccccttatg gctattgcag cccggtataa gttttcgggc
    20401 agatttagta tctcaacatt aaattcattc atcacagaat aattttttag gtagtgcaaa
    20461 aataaacctt aagcttttcc gttaacacaa attaagtatt ttcgcaaaag ttttgcattt
    20521 tagtaattgt aaataaatga accgcaaatt agccttacta tctatatttg gattattact
    20581 tcttttggct tgtaaggaaa agattgaatc tggtgtaagc caacagcttg ccaaccaacg
    20641 taaacaaacc ctttcaaata ttgagtataa catttatatt gatatccctg aaagcgaaaa
    20701 tgctccagtt cccggacgtg ttgagttgat gtttgaacta aatgagcgct acaacctacc
    20761 aatcgatttc aggaatgccg atagctgcct attaaatgta accgttaact atcgccagat
    20821 tagtgctaat gtaaataatg gtcatgttat aattcctaaa agttaccttt accaagggca
    20881 aaataaaatt gcaatcgact ttttggctgg aaaaacctca ctcaaccgca aaggcgatta
    20941 ccttttcagc ttactggtgc ccgacagggc atcaaccgtt ttcccttgct ttgaccaacc
    21001 caacctgaaa gcaagttaca acctaaccct ccaaacccct aaggattggg tagccgtgag
    21061 caattcccct gtaatcaaca aaatggaaac tgacacctca aacctctgga tatttgacaa
    21121 gacctaccct atcagcactt acctttttgc ctttacagcc ggtaagttta aagctattga
    21181 ctttgtagcg gatagtttta aaatgacaat ttaccatcgc gagaccgata cccaaaaggt
    21241 agccagaaat atcaaaacag tgtttgagtt gcaccaaagc gctatcaaat ggctcgaaaa
    21301 ctataccaga atcaattacc ccttccagaa actcgatgtg gttctgattc ccgattttcc
    21361 atacagtggc atggagcatc agggagcaat attttacagg gatagccgta ttttgctcga
    21421 cgaaaattcc tcggttaccc aacaactaaa ccaggcaaac ctaattgctc acgaggtttc
    21481 gcaccagtgg tttgggaacc tggttaccat gaactggttc aacgatgttt ggcttaagga
    21541 ggtatttgcc ggacttatgg ccgataaaat tgttaaccct caatacccca agctaaatca
    21601 tcagctcagc tttgtgctct cacattaccc cagagcctac tcggtggaca gaacaggcgg
    21661 cgcaaaccct atccgccaaa acctcgacaa ccttatgctt gccggaacac tttacggcga
    21721 tatcatctac cataaagcac ccattgccct ccagcaactt gagctgatgc taggtgaaga
    21781 taaattcagg gaaggcctaa atatctacct gaaaaagtat gcatacagca atgccggttg
    21841 gaatgaattg attgaaattt tcgataacct ctcgtccgat gatattaaat cgtggagcaa
    21901 acgttggatt gatgcacctg ggatgcctgc catatatgtt gatatagtga gcgatgtaat
    21961 caatattagc caaaccgaca actggctccc catggagttt gaggttatga tacctaccac
    22021 tgcaggatac caaacagaga aagttaggct taatggaaag cataccgaaa taccgatgcc
    22081 aaaaaatttc acggggttaa aagcaatcat tcctaacagt aatggtttgg ggtatggact
    22141 tttcgtttct gctgatcgcg atctaactct tttacagctc aatgaattag caaacaacga
    22201 tgtggctcgc gcttcattct tcatcaacct tcatgagctt tttctaaatc atttagttga
    22261 taccgataca tacttccgtt tcctggccaa tgcccttagc cgagaaaaag accctcaaat
    22321 aagaaactac ctactaggca atctggaaac tgtgtggtgg caattccttg accacacaaa
    22381 ccaaattata ctttcgcaaa ccctgcagga agccttacag ggaatattta acaacccaaa
    22441 aatacctgcc gatgagcgca aacctgcttt cctgacctat tgcagaacgt ctatcactcc
    22501 tgaggctgtt gaattcattg aaaatatttg ggcaggtaga gttcaaatca aagggatttc
    22561 actaagtgaa aacgacttca tagacctttc gttccagctt gcggtaaggg acgttcacga
    22621 tgccgacagc attctgcagg tgcaggagga aagaattcag aatgccgata ggctagccaa
    22681 gttcaggttt gtaaggcgtg ctgccacacc cgacgaagcc ctacgcgacg actttttcca
    22741 cagcttgctg attgctcaaa accgtaggcc tgagccatgg gttgccgaag ggctgcgact
    22801 cttttttcat ccccttcgag ccaaccattc catccattac ctaaacgctg cactcgacct
    22861 actgcccgaa atacagcaaa ccaacgatat attctttcca aaaacttggc tcgatgcggt
    22921 gctttacggt catagctcta ccgaagcagc aaaaattgta acaaagtgga ttcaggaaca
    22981 cccacaacta tcgcccaacc taaaaggcaa gctaatgcaa tcggccgacc tgcttttcag
    23041 agctgcaggt agcaagtaat tatgcttgca taaacagctg cttggcaaga aactcaacca
    23101 cctttacgtt tacagcattg ccaaactgtt tataggcttg ctgggcgttg ctatttatga
    23161 tgtaattttc agggaaactc tgaaggttag ctacctcacg cggcgttagc cggcgttttt
    23221 cccacccaac aaccgggata tgcaccatag ctaccagtgc tggaaattcc gtaggacgct
    23281 taacccttat acccgagggg cgaaactgta ttattccctt ccaaaccgta tcgatatgtt
    23341 caccggcttg ccactcaaac ttggtgtggg tttgaacaaa ctccgaaagg taattgtact
    23401 ttttcaacca tttatcgata aaaactttgt tttggctgta aagcatgcga ttcttctgta
    23461 caaaaccagc cttccatgtg ggcaaattgc ttaaatcata gtcttttcca aactcattgg
    23521 cccaaatggg aaagccaagc ttaagtctgc ctacaccttg aataaattca tcccatgccg
    23581 atagcacata ctcctcgtaa ttgctgatag caaaccgtct atcggccttg ccgttcagca
    23641 ctattgcccc atctgtttga ttacgatgcg atggcggtat tccaaaatgt agtttcctta
    23701 gttttgtggc tgacgagtga accccaagga tgaaaaccct ctcgcgaagc tggggaaccc
    23761 caacctgatg cggactaaca atgagcggct tttcagtgat gatatagcct aattccttta
    23821 aattttccga aatcactttc caagtccttc ccccatcgtg cgatgcaaga ttgcgaacat
    23881 tctcaagcac aatgtatttg ggtcgatgat gcttcagaat acgctttatt tcaaaaaata
    23941 gcgtaccccg ggtatcgtca aatccctgtt gcttacctgc ttttgaaaag gcttgacaag
    24001 gaaatccggc acatagcaca tcgtgctgtg ggattgagct tacatcaact tcacgaatat
    24061 cacacaaagg gtcaattcca aaattgattc tgtatgtttc agcagcgctt gggtcaattt
    24121 ccgatgcaaa aacacactta cctcccaacc tactcatggc aatatgaaaa ccacctactc
    24181 cggcaaaaag atcgataaac cgaaaactgc tcattctaaa aatttatgcc aaaaatagca
    24241 tttgtttagc tacatttatt ttacttcttg gttttataac cctttttatc atatctacac
    24301 acttaagggg ttccaaacca atttattact ttaatttcaa aaccattgaa ttaagaatat
    24361 ctttgtaata tcaaacataa aagaaatgga taatttaatt gatagcatcc gtagcgacct
    24421 aaaaagccaa gcaaccgagc agaaccgaat tgcgggcgaa agattcttta aagagaatgt
    24481 aaaactttat gggcttaaag ccgcacaggt tcaaaccatt gctaaacatt atttcagcag
    24541 ccaggtaaaa atgatgagta aaagcgagat gcttgctctg agcgaacagc tttggcaatc
    24601 gggattcatg gaagaaacct ttattgcatg ctactttaca gagcgatttg taaaacattt
    24661 ttcgcctgag gactttgtaa cccttgaaga gtgggtaaaa aaatacatta acaactgggc
    24721 gtcgtgcgat accttttgca accatacaat tggcgatatg cttatgttgc atccgcaact
    24781 aattgaaaag gtaaagcttt gggcaaaatc caacaaccga tgggttcgga gagcagcggc
    24841 agttagcttt attgttcccg cccgtaaggg cttatttcac aatcatattg ttgatattgc
    24901 taccatgctc cttaccgatt ccgacgatat ggttcaaaag ggttacggat ggatgttaaa
    24961 agccacgagc caatcaaatc aggaacttgt attcaatttc gtgatgtcga ataaagctaa
    25021 aatgcctcga accgctttaa gatatgccat tgaaaaaatg ccactggaac ttaaagctaa
    25081 agcaatggct aaatagctac accctaacgt tttacctttt acattgaaaa ttttctcacc
    25141 ttttcaacta ttattaaagt gcattttacc aaatttgcgc aaaaattttc tgagatgaaa
    25201 aaatcactgt ttattactgc attttctgtt tttactgcat ttatttcagt agcacaggaa
    25261 caaacccaaa aacctaaaat tgaggttaaa ccttacggtt acgtagcatg ggaaaccatt
    25321 tttgatacct ataagtcagt cgatacacgc gatggcgagc tttacttcta tcccttaaag
    25381 gcaaatatcg atgctaacgg aaccgatgtt aacaaaaagc atcagcttca aatgctttcg
    25441 ctaacaacgc gtttgggatt aaaaatatcc gggcccgatg tacttggcgc caaaactagc
    25501 gcaatggttg aaaccgattt ttttgcaact gcaactgaat atacacgctt acttagggtt
    25561 aggcatgcca taatgaccct taaatggcag cattccgagc tcataatggg acactactgg
    25621 catccgatta ttgtaaacga agtaattccc tcaactcttg cctttggtgc aggcgttccc
    25681 ttccactcgc tgaatcgctc ccctcaaatc cgttttactt actaccccat tgaaaaggta
    25741 aggttatcag caaccgcgtt aacccaaggt taccataaat cgatgggccc agctgaggca
    25801 caacgcaact ccggactacc cgaactcatg gcacaggctg cattcgtaaa aaacaattca
    25861 tttattgctg gtttgtcggc tggttacaaa tggcttactc cccgactcta tattaccagc
    25921 tctaatactg gcacacataa aacagttggt caatacctat ttagtgtatt tacagcagca
    25981 aatctggcta agactaatat caagtttgaa acagtttacg gcgaaaatcc aacccacctt
    26041 cttatgattg gtggctacgg tagggttact gaaaccaccg ctgatagcga ttacgactac
    26101 gcaaacctgc gtactcttag tacatggcta gatgttgacc ataagttggg aaaatggggc
    26161 attggggttt ttgccggata ctcaaagcta ctcggttcca ataagaatta ctcaaccatt
    26221 gccagctacc accgtaacga cgatatgaat tatgtttacc gtatctcgcc acgtgtaagc
    26281 tacaaggaag aaaacctggt gctaggaatt gaatacatgt taaccacagc ggtttacggt
    26341 aaaaccttcg acgctcagca taaggttacc tcatcgctcg accctgtaag caataatcgt
    26401 attacaatta gcgcaaagta ttccttctag attgatttaa tactaaaaca gattccttgt
    26461 atttaatagt ggcttgcgta ttggcaagcc acttttttat cactttatgg ctaactttgc
    26521 ataaaattaa aggcgatggt tcgggtaaaa aaggctttac gcataggttt attactcctg
    26581 gtgttaatta tttctcttct atttctttca cgaaatctta ttgtaaaaaa aatttttagt
    26641 aaaacctctg caagaataaa aaacaggtat ggtttaacag ttactgcaag cgcagttgaa
    26701 gttaaaggct taaacaccct tcagctacaa aacctactta ttgttgatac cgacactttg
    26761 ctttgctctg aatcctttag gattaagctt aatccgttcc atttggtatt acttaaaatc
    26821 aatcccaagc aaattgatat atccagagct acgattaata ttgataattt gattgctcaa
    26881 gcaagcaagc atacacttca aagctcaaat gagagaaaaa atgaaagttc gagcaacaaa
    26941 aggctttacc atttggttaa ggcctttttt ggactttcaa ccgcaacctt caatattaat
    27001 aacctacact ttacctattc cgattctgct tataccggaa aagtaaccat tgaaaaatgg
    27061 agttatagca acaataactt tacctcaacg ctaaccctaa acgatggcga tagggcttca
    27121 cgggtaactg ttaccggaac aaccagcaag cgtaaaaata gccttaccgc aacggtcaca
    27181 accgagcaac ctgaagccta tattcccttt accacacctt ttcttggagt taaagcatcg
    27241 ttcaaaaaaa tcttccttga tttaaatgct acccatattg agcccggtag gatagagtta
    27301 aatttagtat cgagcattaa ttcacttttg ctggatggta agcgtattgc gcaaaagcca
    27361 gttataattg attcatgcgg ggcaaacatg aaaatcaggg caatacccaa tagtttcgtt
    27421 attgactctc tgtcaacagt tagccttaat ggcttcaaga caaatctagg atttgaatat
    27481 aatccattac caaaccgcta tgtaaggttt acactcaaca ctggcgaaaa tagctggcaa
    27541 tcgctctttg atgccttacc taaagggtta ttcacaaacc tatggggtat aaaggtgaac
    27601 ggaactttca gctactcgct tcaggttggt gttcccctca caaaccccga tagcctaacc
    27661 atcgcaccaa acctaaaatc gaaaggattt tatgtgctat cgtacggaaa taccaacctt
    27721 gccatgctcg ccgatacctt tacccatagg gcttatatcg acaatgtgta tgttagagat
    27781 attaaagtta accccaaaag tgcaagctac gcttcacttg accaaattag tccataccta
    27841 caatgggctg taataacaag cgaggatggt ggcttttaca accatcgcgg gttcagcctc
    27901 gatggtataa cctatgcaat ggcatgcaac atacgcgaaa aaaggtttgc tcggggtgga
    27961 agtactatta cccaacagct tgtaaaaaat gtatttctaa atcagagtaa gaatattgga
    28021 cgtaaagccg aagaaattct tataacctgg attacggaaa atagtggtgt agcatcaaaa
    28081 gagagaatgc ttgaggttta cctgaatatc attgagtggg ggcccgatgt gttcggtatt
    28141 cgtgaggcaa gtcaatacta ctttggcaaa aagcccagct cacttacgct tcccgaagct
    28201 ttattccttg cctacataat ccccaggcca gctaagtttc gttacctatt cgataccgaa
    28261 gggaagctta agccctttgt aactgaaagt ttcatgtttg tggcaaataa aatgctaaac
    28321 cgtggaaaca tcactcagca agactacaat aatctggcta ccaatcctct ggtaaccctt
    28381 acagggccag caaaagattt actgaaaaaa gaatcatcat tggaaaatat cgattctgaa
    28441 ggcaatttta ctgaacagga aaaggaaaca aattagcatc aaccccttta catcgcccta
    28501 aaaattcatt aaatttatgg gtgtaaacca aatcggattg atgctatgaa cacacaggta
    28561 tcaaagaata ttgaagcagt tgctgaaagt attcaatggt ttgggcaagc agcagtaaaa
    28621 atcatgaact gcggaaaggt aatatacatc gacccatacc aaataaaagg gaacgataaa
    28681 gccgatttaa ttctaataac tcactcccac tttgaccact tttcgcccga cgatataaaa
    28741 aaaattgtag ccacacatac gtggatactc tgtccatccg atgttgccga tgaagcccaa
    28801 aaatttggag cagccaaggt tattgctgtt cagcctggct ttaacgatga atggcatggc
    28861 attgaaatta agaccgttcc catgtacaac ctaactaaaa ccgacaagca cccaaaatca
    28921 aaaaattggg tgggctacct gcttaacctt tgccatgttt ggctttacca caccggcgat
    28981 actgagcgta ttcccgaaat gaagagcatt aaaactgata taatactgct acccttgggc
    29041 caaacctaca ccatgaacag cgttgacgat gcggttgccg ccgtactcga cactggcgca
    29101 agtgttgcta tccctattca tttcgggatg tatgagggaa ccactgccga tgtggataag
    29161 tttaaggaat tgcttaaaga taaagtcgaa gtgattgtgc taccaaaatc ctagcttgtt
    29221 agtcgaggca ctccacaaac tccagcctcc agttttcaag ctggagtgct gttaggtatc
    29281 ctgttcctgg aatgtaaagt cccttgtaaa cacagccagc gtccaaatca taaagccgac
    29341 gttgagtatc cttaaactct tctactattt ttgctaatgc ccgtggagta tggccatgaa
    29401 taattttata gccgggcata aaactgctag gggcttcggt tggacggttc cacaacatgg
    29461 catactcatc ggcaaatgga tttggcagct catagttaaa acctccatga accagtaggt
    29521 actgattatc gactactaca taaggtttta gagatgtaaa gaaattcaaa tgccgtttag
    29581 gcaaaaaacc ataatctgtc tcaattgctg ttacaattga ctcgtactga tgcgtagtag
    29641 caaaacctga attcattaac cagagactca tctcgtaatc gcccttaatg cagtcgagca
    29701 tcatttgctc gtgattgccc tttagacagg taatattaaa accctcatca attaagttta
    29761 agagatattc tacaacacca ggtgtgtcag ggcctctatc tatataatca cccagaaaga
    29821 atagcctatc ggttttacaa accgaaagtt ttacctccaa taaaaatcga agggtttttg
    29881 aacagccatg aatgtcgggg attataaaag ttctcatggt aatggtttat ataaatttaa
    29941 cacgaaaaat taactttgcc aagttttttg tggttatggc aagcggatac attataaaaa
    30001 aacttcaagg gaaagttaat caggccattt acggttataa tctaattgac cagggcgata
    30061 aaatcatggt tggtttatcg ggtgggaagg atagttatgc tctgctggac ttactggtaa
    30121 accgtcggaa agttcttccc atcggttttg aacttcacgc ttgccacgtt caggctaccg
    30181 atatgaccta ccgcgccgat attgaattca tggagcaata ctgtgcccaa aacaacgtga
    30241 aatttcactt gcgtcaaatc agtgtagagt acaacccaaa cgataggaaa cctgcatgct
    30301 ttatttgcag ctggaaacga cgtaaaatgc tattcacaac tacccgtgag gccggttgta
    30361 ataagctggc actggggcat cacctggacg atgccattga aaccttactc ctcaacatga
    30421 ttaatcactc atcaataagc tctattcccc ctaagctctc aatgtttaac ggcgaggttt
    30481 tcctaatccg acctttaacc acatgcctgg ataaggaact ggaaaagtat agccagttta
    30541 tgggctttcc atcggaaaag gaactttgcc agttcgataa ggatacacac cgtaatactg
    30601 tccgcgaact aatccaacac atggaacaaa taaaccgcga tgcccgcgaa aacatttacc
    30661 gttccatgca aaatatcttc gatgagtata ttgttcccaa gccccgtaaa aaggggaact
    30721 aactatcgcc acgacttaac catgaacagg agcagcaaac caaaaatttc caaacggcca
    30781 agcagcatat cgaacgaaag gacaagcaga gccgaatcgg atagcattga gtaatttgac
    30841 aatgacccaa ccaatccaaa gccagggcct gcatttccca tacaagcagc agtagctgaa
    30901 agcgatgtta aactatcgta tcctaaaccg gtaagaatta gagtgctaac gaataaaacc
    30961 aaaatataga aaacaataaa ctgaaccaca gaatttacaa tctcatcgtt tatcacgctc
    31021 ttccctatcc gaacaggaat aacagcctgt gggtgctgaa tttttaggat ttgacttttc
    31081 agcgcctgga aaaagattac caccctatcg agcttcatac ctccggctgt tgaaccagca
    31141 caagcgcatt gaatggtgaa gaatattatg attagaattg aaaatggtgg ccaaattgat
    31201 gaatcggcat tggcaaaacc tgttgtggtc gataacgata caacttggaa tgctgcatac
    31261 cttatgctct caccccatgt agggtatatt ttaccatgca ggttaattga taccaatgca
    31321 acacctatga tagtaatcca aaagtagtac tttacaattg gggaattaaa aatattattc
    31381 ttttttaggg taattgtgga atacagtaac ccaaaatgaa ttcccgataa gaacatgaac
    31441 actataatga tgatatcaat tggaacacta ttgaaatagg ctacactatt gttttttgtt
    31501 gaaaaacctc ctgttgctac agtggcaaat gcgtggttta ccgcatcgaa caatcccatt
    31561 ccaaacattc tgagcagtat aacctgggta acggttagcc ctaagtaaac caccatgatt
    31621 acattcaaca tttttcgggt tctgtaccta aaattttcct gcgccagcgg cgaaatctcc
    31681 atttttgaaa gggtcatctg ggctttacct aacgatggta gaatgattag cacaaataca
    31741 actattccta tacctcccac ccagtgcatg gatgagcgcc agaacagaat tccttttggt
    31801 agcgcctcaa cattggttag aattgatgcc cctgttgtgg tataaccgga aacactctcg
    31861 aataaggcct taacaaaggt aaactcgcca ccataaagca gtagcggtaa ggaaccaata
    31921 aagcacgata aaatccagct aagaaccaca accaggtaac cctccttatt tgaaaggtca
    31981 gtagccttag ggacaaaaac caaagggaat actccccaaa gcagtgttat tagaaagctg
    32041 aaaagcaagg gcaattgccc agcatcctta ttatacaacg atatcaaaaa cgatacaaag
    32101 ataaatgctg cattaaatag cattaccatt ccaatgtatc ggattatcac atgtggtctc
    32161 attctgtctt cctttgaatg ctcttggtaa gaattagatc cttaactgac tcgttaagca
    32221 catacatttc atcgcgggtt tctattgtta catcgtattg tcttctatac tttgtaaaat
    32281 tctgaatccc ttttgttatt ctttttattg ggcttagcag gtagtatgaa atcatgtagt
    32341 tgaaaataat ggtaaaaata acgctggtaa taacaattat tagtccaggt aaaatggtcc
    32401 tgtaagggct tttttccagt acagaaacag tttggttcag gttttgctgg ttgagggtta
    32461 ataaatcctc cacattgcgt cgcacttctt caagtgcagg attaacatcg gttaggtatt
    32521 tttgaaggtt tggcgaaggt tcattaataa acccttcagc cttagctttg aactttgaga
    32581 atgccaatgc aattgaatca actgtgcttg cctcgccagg tatggtcagg ttgttcgatg
    32641 cctttaacag attgtcgttg aatgttgaca ccgcattgcc aaaaagggaa tttgcctttt
    32701 ccttttcgcc ttggatggat aacataacag ccctttcctg gaggcttaag ctaaagttca
    32761 tctgctttga gaaatcgata ctccggtagt tgtccataag aagcctattg acctgatttc
    32821 ccagcttggt aagctcataa atggatatta accctgaaat aaccagcatt ataccaagta
    32881 tggcaaagcc agttattatc ttgtaacgaa ttttgaacat ggtatcagtg tattattgtc
    32941 ctttatacag tatcacatca gcaaattctg acaatttaaa aatatatgca aatgtataac
    33001 taaagctcaa tagctattca ccaaatacga actaaatgtt cagttcacac aactattcgg
    33061 gaaagataaa cacattttgt aatatcaaaa tctgaaaatc acagtattta cagcatgatg
    33121 ttaaaaaaaa gtaaatgcta gataacatgt agagttttta gtccagcatc aacatacgat
    33181 gacatattat catattaacc tgcgcaagtt ttttgtaact tgtttgtcta ttcatgaaac
    33241 agttttccat tccatggacg agaaagagtt agttcaacaa gttgttcaac ataataatca
    33301 tgaagccttt agcattttag ttgaaaggta caaaaaaatg gttattacca cctgtatggg
    33361 ctttttaggt aactttcacg atgccgaaga tattgcacaa gatgtgtttg ttgagctata
    33421 cgagtcgttg cctgaattcc gaaacgaagc aaaactctcc acctggcttt accgaatagc
    33481 agtaaacaaa tcgcttaact t
//
LOCUS       DAOSAL010000020        33286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_961_length_33286_cov_49.631278, whole genome shotgun sequence.
ACCESSION   DAOSAL010000020 DAOSAL010000000
VERSION     DAOSAL010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 33286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33286
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_961_length_33286_cov_49.631278"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2782)
                     /locus_tag="PK049_04520"
     CDS             complement(<1..2782)
                     /locus_tag="PK049_04520"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOK60857.1"
                     /translation="MSEEILKALMQLFAIIAKQDEGVELKERDYVVNFLTQQLNDEAV
                     KEYIRLFDEHAGLLDKEDNAENDEEKGKRKLTSVKDSVRILGICKKINKTLTQKQKIV
                     VLVRLYELINADRKFTEQRMAIINTVAEVFKLTKEEISDIENFVIKNSPEELDIPSIL
                     TIHDKPFTGKNCKHIGTEALDGYIYILQIKSEDLYFLRYTGNEDIYLNGLPIHNRRIY
                     LFASGSSLKLPKGKPVYYTDVVSHFLADSTSIRISYKVENVGFQFKTGDIGLRNISFS
                     EGHGKLVGIMGASGAGKTTLLNVLAGIEKPTTGRVIVNGYDLHNEKEKLEGVIGVIPQ
                     DDLLIEELTVFENLYYNAKLCFKDKTEEELVALVDKTLANLGLLERKNLKVGTPLNKM
                     ISGGQRKRLNIALELIREPSILFVDEPTSGLSSRDSENVMDLLRELTLKGKLIFVVIH
                     QPSSDIYKMFDNMVILDTGGYMIYYGNPVEAVMYFKRLDMQINSDVGECPVCGNVNPE
                     LIFNIIEARVVDEFGRYTPIRKVSPQKWEELFHENIKLTPIPDEKAEPPKTLNIPSRF
                     KQFLIYTKRDFMSKISNTQYIVLNLLETPILAFILAYVIRYIADPNSDIYIFYENENI
                     PIYLFMALIVALFIGLTVSAEEIFRDRKILKREAFLNLSRSSYLFSKISLLLLLSAIQ
                     TATFVIIANSILEIRGMYFDYWLVLFSTAVCANLLGLNISASFNSAITIYIVIPLLMI
                     PMMVLSGAMFSFEKLNRSVGSFNRVPLIAEFMITKWGYEALVVHQYKDNAFQKIFYDI
                     DKVINNANYKTVYYIDELEKRATDLIDLLPQKDNPEVQKKIADKLAVLRKAIINETTY
                     ESPSITFNSLNKLSINTIDEATVYAIFDYLDRLKSHYSDIYSTQSQKRERMISFLVNN
                     NRELYEAKRRAYD"
     gene            complement(2816..3199)
                     /locus_tag="PK049_04525"
     CDS             complement(2816..3199)
                     /locus_tag="PK049_04525"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOK60858.1"
                     /translation="METIKIMGTDDTPTVILDAQNEIFEISGRSLPEDVTAFYDPILQ
                     WLDDYSAAPKPKTIFTFKLVYFNTASSKLLLDILMKLEQLKEAGHDVLVRWYYPEDDE
                     DMQEAGEEYADIVDVPFEQIGYTLS"
     gene            complement(3210..3785)
                     /locus_tag="PK049_04530"
     CDS             complement(3210..3785)
                     /locus_tag="PK049_04530"
                     /inference="COORDINATES: protein motif:HMM:NF038262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HOK60859.1"
                     /translation="MDRKNIKGFLEFVYDFYKSMKAHEITLVYEGEITHQITKAFTSL
                     TESNMAKEEESNSVQKKVFHVMVECLQNISKHADSFGSDDFLFAGRGIFMVSKGDSEY
                     HVTTGNVIENSKIEELSKILDHINSLDKEGLKQLYKTQMREGRLSEKGGAGLGFIDIA
                     RKTGRKLEYHFLPIDEETHFFVLTSTISRSE"
     gene            complement(3825..6437)
                     /locus_tag="PK049_04535"
     CDS             complement(3825..6437)
                     /locus_tag="PK049_04535"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOK60860.1"
                     /translation="MISFGIISTTDSKQVIRQRVTQAFLVSLMFPMIAWIVDILSRGI
                     PFNLTSIADIHATNPIHWIIDLAPFVISYVTWLQLTRHYRRILLLERELRQRDEDIEK
                     NARFAQRIGEGDYNAPFQVGGDDDILGKSLVIMRDNLLANQKKEAEQNWITKGKDEIS
                     HILRLHNNIDELSYDVLVKLINYIRTIQGAIYLYDEEKQKLVNLATYAYNRRKYIKQE
                     FRIGEGLIGECAYEREFIYRTEVPDNYVTITSGILGDRKPKSLLLVPLITDEKLEGVL
                     EFASLDDEIPELTIRFLKEVGEIIARTIFNLRINQKTEKLLQEAQQMAQELKENEEEL
                     RQNAEEMKITQEELEISNAKLEAKIQEVENAQKRLHSLLENASEIISIYNSEKQLTYI
                     SPSVTKILGYTPQEMMSGKDIDRLTRKGEAAFNEMFEQLLENPRIAITIQYTFMKKDG
                     EKIFLEVTGRNLLDDPAIGGIIINSRDITERKRAEKEERMRSKMQSLSENSPDIIMRV
                     NPAGQFFYANPMVETYLGIDYRELVNQTITALDGIPVLSNFIREAIKTIKVEKEKYEG
                     EITFTTNRGETVMHIVGIPEFNENELETILFVAHDITEQKQTEREIQEKNRKITESIN
                     YAQRIQTSILPNNRIIRQYLPKSFIYYHPRDVVSGDFPWFFIKDDFIYIAAVDCTGHG
                     VPGALLSFIGYFTLNNVVDHDSSYTAGQILDHLHFGVRKTLKQDRPDADARDGMDIAF
                     CKINPKNKELQYAGAHRPLYLVRNGELTEYKGDRKAIGGIPHPKKAEEPFTNYIIEYK
                     PGDKVFFFSDGIVDQVGGPEKKKFGAQSIRDIIMNNLKTPIHELNDVFAKALTDYQGE
                     NKQVDDILLIGIEF"
     gene            complement(6453..7232)
                     /locus_tag="PK049_04540"
     CDS             complement(6453..7232)
                     /locus_tag="PK049_04540"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOK60861.1"
                     /translation="MKPINPTNRFVPVIVLVLLLATSLLVFSLIYNTALETGQKISPW
                     VIGLCLLPFLFGLWFYFIVVKQRNLIDGLQAQIELLKAQVESFMKSQKKETTQEIRKE
                     TLDYKKLLEKIIPNLPTDDIVKYGEALLANVAKTAEIVQGQLYLKNSQTDVFSFKAGY
                     AFFSETTPPEYREGETLAGQVAKNQKLINIDNIPEGYITILSGLGKGSPKHLIIAPII
                     STENTTIGIIELASFKPFTSEHEELFSQLGRKLGEILSVNQ"
     gene            complement(7237..7797)
                     /locus_tag="PK049_04545"
     CDS             complement(7237..7797)
                     /locus_tag="PK049_04545"
                     /inference="COORDINATES: protein motif:HMM:NF013502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HOK60862.1"
                     /translation="MYKAVIIGGSAGSFQVITRILHALPPNYPLPVLLCLHRLKHVRS
                     GFVEALSIKSGIQIIEPNDKEQIKPGKAYLAPANYHMYVELGNKIALSTEEPVNHSRP
                     SIDLSFITAAQVYREKLVGIILSGANRDGAYGLKKIKDLGGLAIVQNPDECQVRTMTE
                     ASLKMTPVDFVFNTNEIIKFLQNIKV"
     gene            complement(7803..8639)
                     /locus_tag="PK049_04550"
     CDS             complement(7803..8639)
                     /locus_tag="PK049_04550"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HOK60863.1"
                     /translation="MTIRYEIGIVDTRNVIKTLLDDYGFDFRDYALTSLKRRLEYIIS
                     LYALRDAEGLIAKLKSSKDFLDQFLYDITPETTEMFRDPSFWRILRDEIIPELVKSGS
                     SKPRIWIASFDSGEELYSLSILLKELNLLNEVNLYCNVLSDHTIQRITSGRLDPKDME
                     VNEANYQRFNEKGKYSSYYRTEANSIIFDVSLIKDVNFIKQNTLIDEDPGAPKLIIFR
                     NQAIYYNQLLSEKVFAKLANCLVAGGYLAIGVKETLENTNVSNKFTLYNEAERIYKRK
                     TN"
     gene            complement(8646..11018)
                     /locus_tag="PK049_04555"
     CDS             complement(8646..11018)
                     /locus_tag="PK049_04555"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOK60864.1"
                     /translation="MKAFANMKLAQKVQLAIIPLMVVLFAFAGLILNYFSRTRTINNA
                     VKEAYVYIDRLIDIANIVEQKTGKGLTSNDIFELKPYFNKPAFNKTDFPSLISRNGEY
                     VLHITRQGARLPMKTIQEIKNLKANEGFIYYQDITDDITENRILVYKYYKPYNAYFAI
                     SVSPTEVLEQVKANRRAMIAIVFLSSLISVIVVWLLLKPITKQLAHLSVRLKQLAQGE
                     IPPAVKVTDRSELGTLAESLNSLIDGLKSTTEFARQIEQNKLDFDFKPLSKNDQLGLA
                     LLQMRESLKRAQQEEAKRKKEDDIRTWINAGLAKFGDILRQNNNNLNLLADNVIQNLV
                     NYLDANQGGIFMFKEYESEKHLELLSAFAYNRKKFLQKSIMLGEGLVGTCAVEKQTIY
                     LKEIPSDYIAITSGLGEATPTSLLIVPLKLEDEIFGVIEIASFNEFAPYQIEFVEKVS
                     ESIASTLNSVKNNIRTRELLEQSQQQREEMAAQEEEMRQNMEEMQATQEEMQRKNLEL
                     EIITSAINQSLVSCSLNDDGFIIDSNLVFQEMTGYSKVELEGMLLESLIVDSQREEFS
                     KLWKSVLNGQSINTTLHLIGNNGRERFLLASLTPGFDPMGLLIKILLMGHDITESKKL
                     ELMTQQQAKEIEKNIKRIKEEQEISNLRQREMETLLKALDQNCLVTIIEPDGLITYIN
                     NKNVEVLGDKKEDIENRFIQDIDFTAKNKPKEFERFWKSLHKGIKQTREFSLDVKGET
                     RWVLENYTPIMDESGNLYKIINIALDITENKRKEMELNAVIEQLKQQLKK"
     gene            complement(11027..11881)
                     /locus_tag="PK049_04560"
     CDS             complement(11027..11881)
                     /locus_tag="PK049_04560"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HOK60865.1"
                     /translation="MPSQRMQVTDQDLQYFVNTIKSVSKYDFSEYSEKSLKRRLQKIL
                     EDYNLDLTSLLTSLRLDRDFLEKVVKEVTVNTTELFRDPPVWHLLRSRILPRFKNNKT
                     INIWHAGCSTGQEVYSMMILLNEMEMLDKAKIFATDLNSDVLDAAKQGVYKYRFNLGY
                     LDNFDKVIKQNPLNFEEYNDVPYEKYFDIDKVRDTITMKKFLTERPIFRKHDLVVDGN
                     IFFAKFDLILCRNVIIYFNYSLQNKVFELFHNNLYPKGILLLGMHETILGPWATRFER
                     LGQAYIKK"
     gene            complement(12025..13083)
                     /locus_tag="PK049_04565"
     CDS             complement(12025..13083)
                     /locus_tag="PK049_04565"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOK60866.1"
                     /translation="MIKNTLRLGFITIAFAFIFAILFMVYSTITFYDPDREITLQASL
                     NPDTVAISTPYQLITWNIGYAGLGDNMDFFYDGGKRTRDTKARTLENLDSIKKFILQN
                     RTDFIFLQEVDLASRRSYNTNQLQIISQAVNYFTFFATNYQVSFVPVPLKNPMGNVNS
                     GIMTLSRFTPMNAVRVQLPGLSPWPNRVFNLRRCLLVTRFPVSNGREFVLINTHNSAF
                     ENDSLKMAEMSFIRQVALSEYEKGNYVIIGGDWNQLPPNFPPDRFGNAYETKAFKPLS
                     IESSFMPGGWQWAFDPSKPTNRYLDRPFSNQCRTVILDFFLLSPNIHITDIATTDLKF
                     RHSDHNPVKLSFVLMPNQ"
     gene            13193..13897
                     /gene="purQ"
                     /locus_tag="PK049_04570"
     CDS             13193..13897
                     /gene="purQ"
                     /locus_tag="PK049_04570"
                     /inference="COORDINATES: protein motif:HMM:NF002957.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HOK60867.1"
                     /translation="MKFGVVVFPGSNCDQDTVYVIKEILEQEVVTLWHKETDFDPNID
                     VIILPGGFSYGDYLRSGALAKFSPIMNRVIDFANNGGYVFGICNGFQILCESGLLPGA
                     LLHNTSQKFICKNVHLVPDSKNTALTKYLPRDRALKVPIAHGEGRYYADKDTLAQMRI
                     NGQILFRYCDERGVVSESANPNGSVENIAGVCNDRHNVFGMMPHPERASDDELGNTDG
                     RLIFESMIKYIRELSN"
     gene            complement(13894..14607)
                     /locus_tag="PK049_04575"
     CDS             complement(13894..14607)
                     /locus_tag="PK049_04575"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOK60868.1"
                     /translation="MNYFYITGTSSGIGLALTRQVLSNDSSYVVGYSRRNVVSHHRYK
                     HIQIDFSQQGAAREVEIPFFPNAKHVVLINNAGILGDVKPIGKIDNEQLEKTFMVNTV
                     SPAILTNRLLALYLPSAKLITVVNISSGAARHAVESWSGYCASKAAIDMFSMVLSAEQ
                     KLLGDSKVRVISLAPGIVDTPMQDSVRKVDPTYFPQSERFHHYKQKGMLASTEQVAKK
                     IVELIGNPPNDPIIDLRML"
     gene            complement(14681..15748)
                     /locus_tag="PK049_04580"
     CDS             complement(14681..15748)
                     /locus_tag="PK049_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOK60869.1"
                     /translation="MHKQTAHIKENPAQRIKAKLALIPVLLGLGVTGVLLYNEFNPRA
                     FDVINFTWKSALWLMLAWLFMVGRDLGYTLRLMILSDGKFRFWKALRVIMLWEFTSAI
                     TPSAIGGTSVAIVYVNKEGLNLGHSSALVMITSFLDELYFVVMFPLLVLLAGYHGLFD
                     IGNTNSMAKELLTFSIIGYSLKLAWVITLVYGLFFNPRGLKWLLLLIFKLPFLRRWKH
                     GANRAGSDIINSSKEYRNKPISFWVKVVLASFLSWSSRFLVVNALLMAFFSIEHNLLI
                     FARQLVMWIMMLVSPTPGGSGFAEFVFTRYLSEFIPVDAALIVPVSVAMALLWRLVTY
                     YPYLFLGSIIFPRWVRRHFIK"
     gene            15924..16859
                     /locus_tag="PK049_04585"
     CDS             15924..16859
                     /locus_tag="PK049_04585"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOK60870.1"
                     /translation="MVIAKNKRAEAHLAIILANIMFGLNYSIAKGVMPNYLSPMAFTL
                     LRVVTASFLFWLISIFDKSDERISKADYPRFIASGLFGISFNQLLFLNGLNYSTPIES
                     SIISTLNPAMVMLIAYFVLSEHINFVKVTGLLIGASGALLLILGKGSLEVGQNHTLGN
                     AMLFLNTLFYAFYLVVVKPLMLKYKPITVMKGVFVVGLISLLPFGVYDLATTRWAVIP
                     PMIYASILFVLLGPTFLAYLLNGWGLRYVQASTVSIYIYSQPVIAALVAVSVGMDSLD
                     AKKITAAALVFAGVYLVSQRPDGWLVSILKRIQRK"
     gene            complement(16828..17973)
                     /locus_tag="PK049_04590"
     CDS             complement(16828..17973)
                     /locus_tag="PK049_04590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOK60871.1"
                     /translation="MEELIRLRHELHRNPELSGQERATGKRIRFFLSRYQPHELYSNI
                     AGEGMAVVYRGKEPGPTVLFRCDMDALPITELSDFKYKSNTPGVSHTCGHDGHMAIVS
                     GLAVRFFENPLEKGKVILFYQPSEENGSGAKESIERLKELDLMPDYAFALHNLPKYKK
                     GSVVLGRYTFTAASKGLIIKLTGRNSHAAFPELGLNPSAATAEIIQHLLVLPTNFKYK
                     NFVLVTLIHVKVGEVAFGTSAGSAVIMATLRAFDDSDMHLLSDNALKTSTQIATRHGL
                     AIETEFTDDFPASVCDPQLTNVVEKIASEQKKEVIYLNEPNRWSEDFAHFTGVCPSVI
                     FGLGAGEDHPDIHSPTYDFPDDLIIDGVDLLDSIAREITSSESFLKY"
     gene            18165..19508
                     /locus_tag="PK049_04595"
     CDS             18165..19508
                     /locus_tag="PK049_04595"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOK60872.1"
                     /translation="MKTRKNLFALLALVGLAGTLLLTSCEKDEEKEMEMPKNIVEVAV
                     SNPQFSILVQALQKANLVTTLQGTGPFTVFAPTNAAFNELFSQLGVSSIDALTADQLT
                     PILLYHVLSGKVESNQLASGYVSTLSPGAGGLGVSLKVDASMLKLNGNVGITAADISA
                     TNGVIHVIDKVLLPPTVVDIALANSSFTSLVAALTKANLVNALKADGPFTVFAPTNDA
                     FSQLFTDLGVSGLDALNAEDLTPILLYHVLGTAVKSTQLQTGYVSTLSAGPNDSKVSL
                     LVDAAAVKLNNNSKIVATDVVGTNGIVHVIDKVILPPTVVDIALANSSFSTLVSVLVK
                     AELVETLKGEGPFTVFAPTNDAFSALFTQIGVSGIDQLSKDDLTPILLYHVVSGNVRS
                     NQISSGNVSTLNGDINVNVGTTVTINENTSVVLVDVQATNGVIHVINKVLLPPAK"
     gene            19625..20581
                     /locus_tag="PK049_04600"
     CDS             19625..20581
                     /locus_tag="PK049_04600"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOK60873.1"
                     /translation="MKNNSIGIYAATVLAMVFWSLSFIWYKEVFQFYKPITLVVGRLS
                     ISAIFLFITVGLIMRKLTKIKASDLKYFLLLAFFEPFLYFMGESFGMTMVSSTLAAVI
                     ISTIPLFSPLGEFYIYKTRVTLMNFVGIMVSVVGVGMVIFHQGFGKLEANPVGIALMI
                     LAVFAALGYSLTLRKLTSAYNVFTIITYQNTIAVIYFLPIFFLFEFNHFMSVGVTISS
                     LMPLVKLGIFASTFAFLLFTYSVKHLGVTKANSFANAIPVLTAIFAYFLLGETLGIVK
                     IAGIAIVVLGLFLSQVPREHARRVFAFIPVGLITRARLRKNE"
     gene            complement(20588..21677)
                     /gene="prfB"
                     /locus_tag="PK049_04605"
     CDS             complement(join(20588..21613,21615..21677))
                     /gene="prfB"
                     /locus_tag="PK049_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_118304733.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOK60874.1"
                     /translation="MTTIEQVKELAKRRDALRRYLDIDKLSIEYEEEQQKTQAPDFWD
                     KPDEAEKQLKKVASIKSWIDGFNKVNSLTEDLQVMFDFAKEGAATEEEIDSHYRQTLQ
                     AIEDLELKNMLGKEEDRLGAIMKINSGAGGTESMDWAAMLMRMYMRWGERNDYEVKVV
                     DVQDGEEAGVKSCTLEFVGEMAYGFLKGENGVHRLVRISPFDSNARRHTSFASVFVSP
                     AVDETIEITINPADIKWDTYRSSGAGGQNVNKVETGVRLYHIPTGIVIENTESRSQFQ
                     NKENAMRLLKSHLYELELRKRREIQAEIEGKKKKIEWGSQIRNYVLHPYKLVKDLRSG
                     YETSDVNAILDGDLNECIKAYLMQFGDE"
     gene            complement(21771..22859)
                     /locus_tag="PK049_04610"
     CDS             complement(21771..22859)
                     /locus_tag="PK049_04610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOK60875.1"
                     /translation="MSNILFIVQLPPPVHGASLMNKLVMGRASQLPNNKCFTISLNFS
                     KSLSDLQRFKPRKILRAITVVFEIIGKIIKYKPDIVYFSMVPIGLVLIRDSFYLMLCK
                     ALSPKSKKIIHLHRPGMLQFYSQKPYLYRFYSFIFKNCEIVHLTPRLARLELSPLNLK
                     KTKITVIPNSIIKTPEYERHEKDWSNILFLSNFLIHKGHFELVEAFAILSSKYPQLKL
                     TLAGAFPSETEEQNLLERIKQYGLNRTVEVVGPVYGPKRFELYSRAGIFVLPSKLEYF
                     PLVILEAMSERCAVITSGKENLQDTFVDGKQLLFLESVDPYDISQKIERLIQSPQLTQ
                     SIANEGYKRFTEIQEESLLKIDKLFTCQ"
     gene            complement(22873..23490)
                     /locus_tag="PK049_04615"
     CDS             complement(22873..23490)
                     /locus_tag="PK049_04615"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019152098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOK60876.1"
                     /translation="MINLSPPRFFQRFFRNVIWNFPSEKEGVFLTFDDGPTPGVTPWV
                     LEQLRKYNAKATFFCLAKNIEMHPEIFEMIVADRHAVGNHSYSHIKGWGTDTTQYVQD
                     IIYANSFVNSNLYRPPYGRITPKQFKLLKSQYKIILWDVLSMDYSRRVSPKRVYQYVV
                     RNAKPGSIIVFHDSLKAERNLRYALPRVLDNLSSKGLTFKSIIIP"
     gene            complement(23493..26561)
                     /locus_tag="PK049_04620"
     CDS             complement(23493..26561)
                     /locus_tag="PK049_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008146873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOK60877.1"
                     /translation="MENLSVSQFKKYNNLIGWFTFAIATLVYLLTIEPTASFWDCSEF
                     IASGNKLEVGHPPGAPFFMLMVRFFTMFAPSPQWVPILANSLSALASSFTILFLFWTI
                     TYMARRIVSPKATEFTLTQVILILSSGFVGALAYTFSDTFWFSAVEGEVYATSSLFTA
                     LVFWAILKWEEQADQPHATRWIILIAFLMGISIGIHLLNLLAIPAIALVYYFRKYSYS
                     PKGLFYTLLISGVLLITIMYGIIQGFVVIASKVELLFVNSFGLPIKSGVFFLFTVVLA
                     LLAYGIYRTHKQGRVVLNTIFVGLTVIMIGYSSYAAIVIRSSANPPMDQNSPDNMFSL
                     LYYLNREQYGDRPLLYGQVFNAQPIARVEGEPTYAPKNGKYVFVRTKDKYEFDPEFKM
                     LFPRMWSNEQNHVNAYKKWSNFDGNPIKRFGPDGKTETIYVPTFGENLLFFFRYQLGF
                     MYFRYFMWNFAGRQNDLQGHGEVTKGNWISGIPFIDNPRLGPQEKLPEIYRNNKGRNQ
                     YYLLPLLLGLAGLLYHLSRHKHDFWVTTMLFVMTGIAIVVYLNQTPYQPRERDYAYAG
                     SFYAFTIWIGLGVLAVYELLKKIKDTPATAVVTASVCTLAVPVLMASENWDDHDRSGS
                     YVPVDFGYNMLIGCKPNAILFTYGDNDTFPLWYSQEVEGVRTDVRVANLSYLRGEWYI
                     EQMQRKAYQSDPIPIRMTYDQYFTGARDAIVVIDRIKEPLNLDRVVDFFLSDKPENMI
                     QSPFNQNERINYIPSKQLFLTVDKSEVKSKVDLPDNYLNRIADTMTWTLNKQVVLKDG
                     QVILDLIATNQWRRPIYYATTVSGETYLGLDKYFHLEGMMYRVLPIPASRQWGTGSVN
                     TREMWENLMEKYRFRSLNNPKVYLDENKMRIVSNYRNLFARLANALVDEGKMDSAITA
                     LNRCMELIPPKNVPLNYFALSMVEGYYRAGDMQNALKYSQQMVNQASEAISYMIYDLD
                     EFQKNWLGNELQLNLAILYELTRMANQYEKGEYAKQLTDKFNSYVTVLEG"
     gene            26684..27316
                     /locus_tag="PK049_04625"
     CDS             26684..27316
                     /locus_tag="PK049_04625"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOK60878.1"
                     /translation="MTSTYRYVFKGLLALLLFGIVVSCSKDDEIVDPTSKPDPEIAQL
                     TKKIHADSIAATIGMLQGMGTRFALAENRRDVAVAIMNKFKSYGYATARLDSFQLNVY
                     FRNQYYETWQYNVVCTLPGVEFPDSLTIIGGHHDCILRSTEPESPLDVAPGADDNASG
                     VAVTLEIARVLKQSGFAPRTTIVFATFAAEELGLYGSQHMTNRLSDTLAR"
     gene            27322..27660
                     /locus_tag="PK049_04630"
     CDS             27322..27660
                     /locus_tag="PK049_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60879.1"
                     /translation="MLNNDMVSFCPVADSTGWYLNVIDYPNSVDFRKHAQKICSTYTL
                     LKTVNDNTYRNYSDSYSFYLRSYKSLFFISQHDDPNYHKLTDLLSNQNPRFAREVAKL
                     NCALIVEANK"
     gene            27714..28958
                     /locus_tag="PK049_04635"
     CDS             27714..28958
                     /locus_tag="PK049_04635"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOK60880.1"
                     /translation="MKRTVVILVGLVIIAFAAYILLKPRLAQNSYNEGLKLLDSAKYE
                     LALEKFNRAIKLNPKRYDYFFKRGVTLAALNNDSLALADFSTAIGIDSTHFELYIERG
                     VSYRKLKQYSQAMTDLNRAIAMNDSSSKAYYQRGLLNAATMDFEQAIKDYNRSIELDG
                     SNLEAFYSRGESLANLADFKGAIADYDKMIEGANATPEAYKKRGIFKLKIQDYNGAIA
                     DLEVALKELPKDEEALYSLGDAFANVQRFDDAIKVLDKAIAVNPKLPLAYGVKGGALL
                     RKGEFKQAVVAFNKAIELKPDYLKAYYGRGVARAFQKDYKGSITDFNKVISIDPNYVQ
                     AYYNRAVSKAIMGNHKDAIPDYDVIIKADPKNAEAYYNRGISKMNVQEFKSGCDDFRK
                     AVELGYKPAEQMVNIYCPKENK"
     gene            29028..29282
                     /locus_tag="PK049_04640"
     CDS             29028..29282
                     /locus_tag="PK049_04640"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HOK60881.1"
                     /translation="MMAANFTEFRTSLKKFLDNVVNNNETLIIKRGTGKGAVMMSLDE
                     YNSIMETLHLLNSKANADRLYESIKQMKEGNVVRNKLIEE"
     gene            29279..29542
                     /locus_tag="PK049_04645"
     CDS             29279..29542
                     /locus_tag="PK049_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018712249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HOK60882.1"
                     /translation="MTRITFSKNSWEEYISWQNEDKKMLKKINELIKDIQKHPYEGIG
                     KPEPLKYDLAGLWSRRIDREHRLVYQVIDQEILIYSCRYHYDR"
     gene            complement(29716..30783)
                     /locus_tag="PK049_04650"
     CDS             complement(29716..30783)
                     /locus_tag="PK049_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60883.1"
                     /translation="MKSIMLPIALISTFIAIVYKSNAQPDSSGVGLYLLGQSFFDNKE
                     FTGNIKKGYTHPGFYIQPAIKFKTEQYSIAAGFHTLYLAGADSLERLVPVFSASLQLN
                     SRVTLIVGTLESKNGHWLPEPLFKPERLFLNQPETGVQFLYRGTESKADLWINWERYI
                     KTGSPFQEEFTVGFTHLYHRNNNTGFSNAFHAMAFHHGGQIDSTNLPVQTHINLGVIP
                     AYSFQPLAKTRFEFSSSLYYYKNLSPSFTSYYSSGFGLHPQLRAYHNSTTIDIGYWYS
                     DGFVNPRGEELFGSISTINPTFNQAVRTMGTFSIEFQKVFREKLLLNANFKAYYDFKS
                     GLLEYSYTLRLMFDGKLTRVK"
     gene            30882..32048
                     /locus_tag="PK049_04655"
     CDS             30882..32048
                     /locus_tag="PK049_04655"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOK60884.1"
                     /translation="MNWKRKLKVASVVVVVALAVYLALPQNHYIVRALIYQQVNIDDY
                     KIFHNRTVKAGTPQPWRLHEKYNSYQLTNDQLKYFNDFRTVAFLVVKDTALLFESYWD
                     GYGKDSYSNSFSMAKSIVSLLMGCALSDGYVKSIDEPVGNYLPEFAEGDKAKITIKHL
                     LTMSSGLSWDESYSTLFSLTTQGYYGKNLPKLVLNQTVVKEPGKTFEYRSGDTQLLSL
                     IIEKATGKTLADYASEKIWSKIGAEHDALWCLDRKDGVEKAFCCFNTNARDFARFGQL
                     VLNGGRWNGEQIVPEEYLNESLTPASYLFDPETNKNVDFYGYQWWMINVDGYKTWYAR
                     GLLGQYIFVIPSLNAVVVRLGHVRNNNRIDGTPEDVYQYIRFGISIVKSIENNE"
     gene            32041..33234
                     /locus_tag="PK049_04660"
     CDS             32041..33234
                     /locus_tag="PK049_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60885.1"
                     /translation="MSSNDFIKDRLRVKLLAFPAAELSVFDKAKKKYMGLFNPEEMEF
                     VNDKPDVLMFLTGGSEHIAIESVQEYRFYLLLASREANAWAAATEVKAWMNQHNIRSV
                     LLDADNPSTAEYLSEYYNAFVGVRKLHGQRLGVVGSSSPWLVASGVNPYLLQSKLGVE
                     QVDISWDDIVFDEISQVSPDFTSLFGMAENRVELIESGKIYEGLASLIPFYRLNAMAV
                     ECFPLVNKTGHTACLALAKLNYDGIPTACEADICSAAAMMLSSEVCNAIPWMANVAHV
                     GNGKAILTHCTAPVNHLTSFKLDTHFETGKGYAVSGQLKGDEVTIFRFDSTLNHIFVT
                     LARVTDKPKSRNACRTQLEVELSPSASKYFTETPFGNHHMVLPGNWVQRLTLAAELLN
                     VGVVS"
BASE COUNT         8975 a         6951 c         7546 g         9814 t
ORIGIN      
        1 tatcgtaggc acgacgcttg gcctcgtaga gttcccggtt attatttaca aggaacgata
       61 tcattcgctc acgcttctgc gattgggtgc tatagatatc ggagtagtgg ctcttaagcc
      121 tgtcgaggta gtcgaatatg gcgtaaacgg ttgcttcgtc tattgtgttg attgataact
      181 tgttaagact gttgaaggtt attgagggcg actcataggt ggtttcattt attattgcct
      241 ttcggagaac agctagctta tcagcaatct ttttctgcac ttcagggttg tccttttggg
      301 gcaataaatc tatgaggtcg gtagcgcgct tttccagctc atcgatgtag taaaccgttt
      361 tgtagttggc attattgatc accttgtcga tgtcgtagaa aattttttgg aaggcattat
      421 ccttatactg gtgaacaaca agtgcctcgt agccccactt ggtaatcatg aactctgcaa
      481 taagcgggac gcggttaaag ctacctactg aacggttaag tttctcgaag ctgaacatgg
      541 cacccgagag caccatcatg ggaatcatta gcagaggaat gacaatgtag atggtgatgg
      601 cagagttaaa cgaggcggag atatttaggc caagtaggtt tgcgcaaacg gctgtactga
      661 aaagtactag ccagtagtca aagtacatgc ctcggatttc cagaatgcta ttggcaataa
      721 ttacaaaagt ggcagtttgg atggccgata gcaaaagcag gagcgatatt ttagaaaata
      781 ggtaactcga tcggcttagg ttaaggaacg cttcgcgttt aagtatttta cggtcgcgga
      841 aaatttcctc ggcactaacc gttaagccaa taaacagagc tactattagt gccatgaata
      901 ggtatatggg gatgttctcg ttctcgtaaa agatgtaaat gtcagagttt gggtcggcta
      961 tgtaccgaat aacgtaagcc agaatgaacg ctaaaattgg ggtctcaaga aggttaagaa
     1021 caatgtactg ggtgttgctt atcttggaca tgaagtcacg cttggtgtag atgaggaact
     1081 gtttaaaacg cgaggggatg ttcagggttt tgggtggctc tgctttctca tcgggtatgg
     1141 gggttagctt aatgttttca tggaatagtt cttcccactt ttgtggcgat accttgcgta
     1201 tgggggtgta acggccaaat tcatcaacta cgcgtgcctc aatgatgttg aagattagtt
     1261 ccgggttaac attcccacaa acagggcatt cgccaacatc gctgttaatc tgcatatcaa
     1321 gacgtttgaa atacatcacg gcctcaacgg ggttgccgta gtaaatcatg tatccgcctg
     1381 tatccagaat aaccatgtta tcgaacattt tgtaaatgtc cgatgagggt tgatgaataa
     1441 ccacaaatat gagtttcccc ttgagcgtaa gctcacggag taggtccatt acgttttcag
     1501 agtcgcgcga cgaaagcccc gatgtaggct catcaacaaa aaggatggag ggctcgcgaa
     1561 ttagctcaag ggctatgttt aaccttttac gctgaccgcc cgatatcatc ttgttgagcg
     1621 gggttccaac cttgaggttt ttacgctcta aaaggccaag atttgcaagg gttttatcca
     1681 caagggcaac cagttcctcc tccgttttat ccttaaagca aagctttgcg ttgtagtata
     1741 ggttttcaaa tacggttaac tcctcaatga gcaaatcgtc ttgcgggata acgccaataa
     1801 caccttctag tttctccttt tcattgtgaa ggtcgtagcc gttaacaata actcggcctg
     1861 tggtaggctt ttcgataccg gcaagtacgt ttagcagtgt ggttttacca gcaccactgg
     1921 ctcccatgat accaaccagc ttaccatgcc cctcggaaaa ggaaatgttt cttaatccga
     1981 tatcgcctgt tttaaactga aaaccaacat tttctacttt gtaggaaatg cggattgatg
     2041 tggagtcggc caggaagtgg gaaaccacat cggtgtaata aaccggcttc cctttaggta
     2101 gtttaagtga actcccgctg gcaaaaagat agatgcggcg gttgtgaatg ggtaatccat
     2161 taaggtaaat atcctcattg cctgtatacc ggaggaagta aaggtcctcg ctttttattt
     2221 ggagaatata tatgtaacca tccagggctt cggtgccaat gtgcttgcag tttttgcccg
     2281 taaagggttt atcgtgaatg gttaaaatac ttggaatgtc taactcttcg ggtgaatttt
     2341 ttataacaaa attttcaata tccgatattt cttccttggt aagcttgaat acttcggcta
     2401 cggtattgat aatagccatt cgttgttcag taaacttgcg gtcggcattg ataagctcgt
     2461 acaaacgaac cagaacaact attttttgtt tctgggtaag agttttgttg attttcttac
     2521 aaatacccag aatacgaacg gaatccttta ccgaagtaag ttttcgtttg cccttttcct
     2581 catcgttttc ggcattgtct tctttgtcga gcaggcctgc atgctcatca aagagtctga
     2641 tgtattcctt tactgcctca tcgttgagtt gctgggtgag gaaattaaca acatagtcac
     2701 gctccttgag ctcaacacct tcgtcctgct ttgcaatgat tgcaaagagt tgcattaacg
     2761 ctttgagaat ctcctcactc attcttcttc acttgggcaa aaaacggaaa agaacctaac
     2821 tcaaggtgta gccaatctgt tcaaaaggaa catcaactat atcggcgtac tcctcgccag
     2881 cttcctgcat atcctcgtcg tcctcagggt agtaccaacg cacaagtacg tcgtgacccg
     2941 cctccttgag ctgttcaagt ttcatcagaa tatcgaggag cagcttagag ctggccgtgt
     3001 taaagtacac cagcttaaag gtaaagatgg ttttgggttt tggcgctgct gagtagtcat
     3061 cgagccactg cagaattgga tcgtagaatg cagtaacgtc ctcgggcaag gaacgacccg
     3121 agatctcgaa gatttcgttt tgagcatcca gaattacggt tggggtatca tcggtcccca
     3181 ttatttttat ggtttccata tcggtactgt tattctgatc ttgaaatggt tgaagttaaa
     3241 acaaagaaat gggtttcctc gtcgatgggg aggaagtggt actcaagctt ccttccggtt
     3301 tttcgggcta tgtcaatgaa acccaatccg gctccaccct tctcggaaag gcggccttcg
     3361 cgcatctggg ttttgtagag ttgcttcaaa ccctccttgt cgagcgagtt aatgtgatca
     3421 aggatttttg agagctcctc aatcttggag ttttcaatca cattgccagt agtaacgtgg
     3481 tattccgaat cgcctttgct aaccatgaaa ataccacggc cagcaaagag aaaatcatcg
     3541 gagccgaaac tatcggcatg tttgctgatg ttctggaggc attccaccat tacatggaaa
     3601 acctttttct gaacggagtt cgactcctcc tccttggcca tatttgactc ggtaagggag
     3661 gtgaaggctt tggttatttg atgggttatt tcaccctcgt aaacgagagt aatttcatga
     3721 gccttcatcg acttgtaaaa gtcgtaaacg aactcaagaa accctttaat attttttcga
     3781 tccatagccc gttgagttat atgagttaca aatatattac atttttaaaa ttcaatacca
     3841 attaaaagta tatcgtcaac ctgtttattt tcaccctggt aatcggtaag tgcttttgca
     3901 aacacgtcgt taagttcgtg aatcggtgtt tttaggttgt tcataatgat atcacgaata
     3961 ctttgagctc caaacttttt cttttcaggg ccaccaacct ggtcaactat accatcggaa
     4021 aagaagaaaa ccttgtcgcc cggtttatac tctatgatat agttggtaaa tggttcttcg
     4081 gcctttttgg gatgaggaat accgccaatt gctttgcggt cgcccttgta ttcagttagt
     4141 tctccattcc gtacaaggta aagcggacgg tgagcccctg catactggag ttccttgttt
     4201 ttggggttga ttttgcaaaa agcaatatcc ataccatcgc gggcatcggc atcggggcga
     4261 tcctgcttaa gggttttacg aaccccaaaa tgtagatggt caagtatttg accagctgtg
     4321 tacgatgagt cgtggtcaac cacgttgttc agtgtaaagt aaccaataaa ggagagcaga
     4381 gcgcctggaa ccccatggcc agtacagtca acagcagcta tgtaaatgaa atcgtccttt
     4441 atgaagaacc aggggaagtc gccactaaca acatctctgg ggtggtagta aatgaacgat
     4501 ttgggcaggt actgcctaat gattctgtta tttggtagga ttgatgtttg aatacgctgg
     4561 gcgtagttaa tactttcggt gattttgcgg ttcttttcct gaatttccct ttcggtttgc
     4621 ttttgctcgg ttatatcgtg ggcaacaaac agtatggtct caagctcgtt ctcattgaat
     4681 tcagggatgc caacaatgtg cattaccgtt tcgccccgat tggtggtaaa agtaatttcg
     4741 ccttcgtact tttccttttc aaccttaatg gtttttatgg cttcgcgaat aaagttcgaa
     4801 agcactggaa taccgtcaag cgcggttatt gtttggttaa ccaattccct gtagtcaatt
     4861 ccaaggtagg tttccaccat ggggtttgca tagaagaatt gcccggctgg gtttacgcgc
     4921 atgataatat cgggggagtt ctcggaaagc gattgcattt tgctacgcat gcgctcctct
     4981 ttctcggcgc gtttacgttc agtaatatcg cgtgagttga tgattatacc accaatggca
     5041 gggtcgtcga gcaaattacg accggttact tcaaggaaaa tcttttcacc atcctttttc
     5101 ataaaggtgt actggatggt gatagcaata cggggattct ccaggagctg ctcaaacatt
     5161 tcgttaaaag cggcttcgcc cttgcgggtt aggcgatcga tatccttgcc gctcatcatt
     5221 tcctgcgggg tataacccaa aattttggtt accgaggggc ttatgtaggt tagctgtttc
     5281 tcgctattat atatcgatat aatttccgat gcgttttcaa ggagggagtg taaacgtttt
     5341 tgggcatttt caacttcctg gattttagct tcgagcttag cattcgatat ctcaagttct
     5401 tcttgagtta tcttcatctc ctcggcattc tggcgaagtt cttcctcgtt ctccttaagt
     5461 tcttgagcca tttgctgggc ttcctgcagt aatttttcag ttttttggtt tatgcgcagg
     5521 ttaaagatgg tgcgggctat tatttcaccc acctctttca ggaatcgaat agtcagctca
     5581 ggtatctcat cgtcgagcga ggcaaattct aaaaccccct caagtttctc atcggtgata
     5641 aggggtacaa gcagtaaact tttgggttta cgatcgccca aaatacctga ggtaatggtg
     5701 acataattat cgggtacctc agtccggtaa ataaattcac gctcgtaagc gcactcgcct
     5761 ataagcccct cgccaatacg aaactcctgc ttaatgtact tacggcggtt gtatgcatac
     5821 gtggcaaggt taacaagttt ctgcttctct tcatcgtaaa ggtaaattgc cccctgtatg
     5881 gttcgtatgt agtttatcag cttaaccagt acgtcgtacg atagctcatc aatattattg
     5941 tgtaggcgta ggatgtgcga tatttcatct ttccccttgg ttatccagtt ctgctcagcc
     6001 tcttttttct ggtttgcaag caggttgtcg cgcatgataa ccaacgactt cccaaggata
     6061 tcatcgtcac caccaacctg aaaaggtgca ttatagtcgc cttcgccaat acgttgggca
     6121 aagcgtgcat ttttctcaat gtcctcatcg cgttgccgga gttccctctc cagcaaaaga
     6181 atcctacggt agtgtcgggt aagctgtagc cacgtaacat aggaaattac aaagggagcc
     6241 aaatcaataa tccaatgaat aggattggtg gcatgtatat ctgcgatgct ggtcaagtta
     6301 aaagggattc ctctggatag gatatcaaca atccaggcaa tcatggggaa cataaggcta
     6361 accaggaagg cttgagttac cctttgccgg attacttgtt tgctatcggt ggtcgatata
     6421 attccgaatg aaatcatgcc gcagtttggt tattattgat tgacacttaa tatctctcca
     6481 agtttacgtc cgagctgaga aaaaagttcc tcgtgttcgc tagtaaaggg tttgaatgag
     6541 gctagctcta ttatcccaat agttgtattt tctgtagaga ttattggggc aataattaaa
     6601 tgtttaggcg aacccttacc caggccggat agtatagtaa tgtaaccttc gggtatatta
     6661 tcaatgttta taagtttttg gtttttggcc acctgtcctg cgagggtttc cccttcccgg
     6721 tattcgggtg gggtagtctc ggaaaagaaa gcgtagccag ctttaaagct aaaaacatcg
     6781 gtttgtgaat ttttaaggta tagttgcccc tgaacaattt cagcggtttt ggcaacattg
     6841 gcaaggagcg cctcgccata ttttacaata tcgtcagtag gaaggttagg gatgattttt
     6901 tcaagcaatt ttttgtagtc aagggtttct tttcggattt cctgagtggt ttcctttttc
     6961 tgcgatttca tgaatgattc aacctgcgct tttagcaatt caatttgggc ttgaaggcca
     7021 tcgataaggt ttcgttgctt aactacaata aagtagaacc acaatccaaa aagaaagggg
     7081 agtaggcaaa ggccgataac ccaaggagat attttttgcc cggtttccaa tgccgtgtta
     7141 tagatcaagc tgaaaaccaa tagggaagtg gctagcagca aaaccaatac tataacaggt
     7201 acaaatcggt tagtcgggtt aattggtttc atgtttttat actttgatgt tttgaagaaa
     7261 cttaataatc tcgttagtat taaaaacaaa gtcaacaggc gtcatcttga gcgaagcttc
     7321 ggtcatagtt ctaacctggc attcatcagg gttttggaca atggctagcc cacctaagtc
     7381 ctttatcttt tttagcccgt atgcgccatc gcggtttgct ccggagagaa taattcccac
     7441 taatttttca cggtaaacct gagcagcggt aataaatgaa aggtcgatgc tggggcgtga
     7501 atggttaacc ggctcctcgg ttgaaagggc tattttattt cctaattcca catacatgtg
     7561 gtaattagct ggagccaggt atgctttgcc gggtttgatc tgttccttat cgtttggttc
     7621 aataatttga atgcccgatt tgatggaaag ggcctcaaca aatccgctgc gaacatgctt
     7681 taacctatgc aggcaaagaa ggacaggtag agggtagttt gggggcaaag cgtgtagtat
     7741 tcgggtaata acctgaaagc ttcctgctga tcctcctatg ataactgctt tatacataaa
     7801 aactagttgg tttttcgctt gtaaattctt tcagcctcgt tgtacagagt aaatttatta
     7861 ctaacgttgg tattttccaa agtttcttta accccaattg ctaaataacc cccagctacc
     7921 aggcagttgg caagtttggc gaaaaccttc tcgctgagaa gctggttgta gtaaattgcc
     7981 tggttacgga agattataag ctttggtgca cccggatcct cgtcaattaa tgtattttgc
     8041 ttgatgaagt ttacatcctt aataaggcta acgtcaaaaa taatgctgtt tgcttcggtt
     8101 cggtagtacg acgagtactt ccctttctcg ttaaaacgtt ggtagtttgc ctcattcacc
     8161 tccatatctt taggatccaa gcgacccgat gtgatgcgct gtatggtgtg atcggagagt
     8221 acgttgcagt ataaatttac ctcgttaagt aaattcaatt cctttagaag gattgataac
     8281 gagtatagct cttcgcccga gtcgaacgat gcaatccata tgcggggttt tgatgagccc
     8341 gacttaacca gttcaggaat gatttcatcc cttagaattc tccagaacga tggatcgcgg
     8401 aacatttcgg tggtttcagg ggtaatatcg taaaggaatt ggtctaaaaa gtccttgcta
     8461 ctctttagct tggcaataag gccctcggca tcgcgaagcg cgtaaagcga tatgatgtac
     8521 tccagcctcc gctttagtga ggtaagggca taatcgcgga aatcgaagcc ataatcatcg
     8581 agcaacgttt tgattacgtt ccgggtgtca acaattccta tttcgtaacg tatggtcata
     8641 cttcactatt tcttgagctg ttgcttaagc tgttcaataa cagcgtttag ttccatttct
     8701 ttccgtttat tctcggttat atccagggca atatttatta ttttgtaaag gttaccactt
     8761 tcatccatga ttggggtgta gttttccagc acccagcggg tttcgccttt tacatcaagt
     8821 gagaactcac gagtttgttt gattccctta tgaagcgatt tccaaaaacg ttcaaattct
     8881 ttgggtttat ttttagcggt aaaatcaata tcctgaatga atcggttctc aatgtcttcc
     8941 tttttatcgc ccagcacctc tacatttttg ttattgatgt aggtaataag gccatcgggc
     9001 tctattatgg taaccaggca gttttggtca agagccttga gcagggtttc catctcgcgc
     9061 tggcgcaggt tcgatatctc ctgctcttcc ttaatgcgct tgatattctt ttcaatttct
     9121 ttggcttgct gctgggtcat caattcaagt ttctttgact cggttatatc gtgccccata
     9181 aggagtatct tgattagcaa tcccatggga tcgaaaccgg gtgtgagtga agccagcagg
     9241 aagcgttccc tgccattatt cccaatcagg tgaagggttg tattgattga ttgtccgtta
     9301 aggacagatt tccatagctt gctaaactct tcgcgctgtg agtcgacaat taagctctca
     9361 agcagcattc cctcaagttc aaccttgctg tagccggtca tctcctggaa taccaggttg
     9421 gagtcgataa taaaaccatc gtcgttaagt gagcatgata ccaacgattg gttaatggct
     9481 gaggtgatga tttcgagttc aaggttctta cgctgcattt cctcctgggt ggcctgcatt
     9541 tcctccatgt tctggcgcat ctcttcttcc tgtgcagcca tttcctcgcg ttgctgttgc
     9601 gattgttcaa gcagctcacg ggttcggata ttattcttaa ccgagttcag ggttgaggct
     9661 atgctctcgc taaccttttc aacaaactca atttggtatg gggcaaactc attgaacgac
     9721 gctatctcta taacaccaaa aatctcatcc tcaagcttaa ggggaacaat gagcagcgat
     9781 gttggggtgg cctcacccaa cccggaggta atggcaatgt aatcgctggg gatttctttc
     9841 aggtaaatgg tttgcttctc aacagcacag gtgcccacaa gtccctcgcc aagcataata
     9901 cttttttgaa ggaatttctt ccggttatag gcaaaggccg aaaggagttc gaggtgtttt
     9961 tccgattcgt actccttaaa catgaaaatt cctccctggt tagcatcaag gtagtttacc
    10021 aggttttgga taacattatc ggcaaggagg ttaaggttat tgttgttttg acgaagtata
    10081 tcgccaaact tggcaagacc tgcgttaatc catgtgcgga tatcatcctc ctttttacgc
    10141 ttagcctctt cctgttgggc tcgttttagg ctttcgcgca tttgtagtaa agccaatcca
    10201 agctgatcgt ttttgctgag aggcttaaag tcaaaatcga gtttgttttg ttcaatttgc
    10261 cgtgcaaatt cagtagtact cttaagcccg tcgattaagc tgtttagcga ctcggctaag
    10321 gttcccagct cgctcctgtc ggtaacctta acagctggag gaatctcgcc ctgtgcaagc
    10381 tgttttagtc gaacggagag gtgggccagc tgttttgtga tgggtttaag caaaagccat
    10441 acaacaatta cagatatcaa cgaagagagg aaaacgatgg caatcatggc tctcctgtta
    10501 gccttgacct gctcaagaac ttcggttgga cttactgaaa tggcaaagta ggcattgtat
    10561 ggtttgtagt acttgtaaac aagtattcgg ttctccgtaa tgtcatcggt aatatcctgg
    10621 tagtatatga agccctcgtt ggctttgagg ttcttaattt cctgaatggt cttcattggg
    10681 agtcttgctc cctggcgggt aatgtgtaaa acgtattcgc cgttcctgga aatcaatgag
    10741 gggaaatcgg ttttgttgaa tgctggctta ttgaaatagg gctttagttc aaagatatcg
    10801 tttgaggtta aacctttccc agttttttgc tcaactatgt tagcaatgtc aattaaccta
    10861 tcaatgtaaa cgtaagcctc tttaacggcg ttattaatgg ttctggtacg ggaaaagtaa
    10921 tttaggatta aacctgcaaa ggcaaagagc acaaccatta aagggattat ggcaagttga
    10981 accttttgtg caagtttcat gttagcaaaa gccttcatgg taaaatctat ttttttatgt
    11041 aagcctgacc taatcgttca aaacgcgtgg cccaagggcc aagtatggtt tcgtgcatcc
    11101 caagcagtaa tatccccttg gggtaaagat tattatgaaa gagttcaaaa accttgttct
    11161 ggagggagta gttaaagtaa atgattacgt tacggcaaag tataaggtcg aacttggcaa
    11221 aaaagatatt gccatcgacc acaaggtcgt gtttgcggaa aatgggacgc tcggttaaga
    11281 actttttcat ggtgatagta tctcttacct tatcgatatc gaagtacttt tcgtatggaa
    11341 cgtcgttata ctcctcaaag ttgagtgggt tttgtttaat aaccttatcg aaattatcca
    11401 gatagccgag gttaaagcgg tatttgtata ctccttgctt agcagcatcc agcacatcgg
    11461 agtttaggtc ggttgcaaat atttttgcct tgtcgagcat ttccatctcg ttcaggagaa
    11521 tcatcatgga gtaaacctcc tgtccggtgg agcaaccggc atgccaaata tttatggttt
    11581 tgttgttctt gaatcggggt agaatacggg agcgcaaaag atgccaaacc ggtggatcgc
    11641 ggaatagctc tgttgtatta accgttactt ccttgaccac tttctccaga aaatccctat
    11701 cgagccggag cgaggtaagc agggaggtta ggtcgaggtt gtaatcttca agaatttttt
    11761 gcaaacggcg cttaagcgac ttctccgaat actcggaaaa gtcatacttt gaaacacttt
    11821 taatagtatt tacaaaatac tgtaaatcct ggtcggttac ctgcatacgc tggcttggca
    11881 ttatctcatt aagcagatat catcaacaat aatcaggcta aatatagatt tttcttaaat
    11941 acaaacaaaa tctaaatggc aaagatagca ttaactttga ttaacatcac ctaatggatt
    12001 ataagattgc attttccatt gatactattg gtttggcatg agaacaaatg atagtttaac
    12061 ggggttatga tcggaatgcc tgaactttaa gtcggtggtt gcgatatcgg taatatgaat
    12121 attaggcgaa agcaggaaga aatcgagaat aaccgttcta cactggttac tgaacggcct
    12181 atccagatat cgattagtag gctttgatgg gtcaaaagcc cattgccatc cgcctggcat
    12241 aaagctgctt tcaatggaaa gaggtttgaa agccttggtt tcgtatgcat ttccgaacct
    12301 gtcgggcgga aaattgggcg gtagttgatt ccagtcgcct ccaattatca cataatttcc
    12361 cttttcatac tcgcttaatg ctacctggcg aatgaaactc atttctgcca tcttgaggct
    12421 atcgttttca aatgctgagt tatgggtatt gataagcaca aattcacggc cattacttac
    12481 cgggaaacgg gtaaccagca ggcagcgacg taggttgaaa accctgttag gccatggcga
    12541 taggccaggc agctgaactc ttaccgcatt cattggggta aagcggctaa gagtcataat
    12601 tcctgaattt acattgccca ttgggttttt aagtggaacc ggaacaaatg acacctgata
    12661 gttggtggca aagaaggtaa agtagttaac cgcttggcta ataatttgaa gttgattggt
    12721 gttgtatgat cgacgcgatg ccaaatctac ttcctgaaga aaaataaaat cggtgcggtt
    12781 ttgtagaatg aattttttaa tggaatcgag gttttctaat gttcgtgctt tagtatcgcg
    12841 ggttcgcttt cccccatcat aaaaaaagtc catattgtcg cctaatccgg cataaccaat
    12901 gttccaggta atcagttggt acggcgtact tatagcaact gtgtcggggt ttagtgaagc
    12961 ttgaagggta atttccctat caggatcgta gaaggttatg gtgctgtaca ccataaatag
    13021 gattgcaaag ataaatgcaa aagctatagt tataaagcca agacgtagag tgtttttaat
    13081 cattttttta ataattgatt atcaaattta aaccaaaaat gttattggtt caaacctaat
    13141 tttgacgatt ttatattatt ggctatcttt gacgcatatt aattgcatta gcatgaagtt
    13201 tggagtagtt gtattccctg gttccaactg cgatcaggat acagtatatg taattaagga
    13261 gattcttgag caggaagtag tcaccctttg gcacaaggaa accgattttg atcccaatat
    13321 tgatgtgata atactgccgg gcggattctc ctatggcgat tacttacgat caggggccct
    13381 ggccaagttt tcgccaataa tgaacagggt aattgatttt gccaataatg ggggttacgt
    13441 gtttggtatt tgcaatggtt tccaaattct ttgcgagagt ggacttttac cgggagcatt
    13501 actgcacaac accagccaaa agttcatatg caagaatgtt cacctggtac ctgacagcaa
    13561 gaacactgct cttacaaagt acttaccccg cgacagggcg cttaaggttc ccattgccca
    13621 tggcgagggt cgttactacg ccgataagga tacacttgcc caaatgcgaa ttaacggtca
    13681 aatacttttc cgctattgcg atgagagggg agtggtttcg gaaagtgcca atcctaatgg
    13741 ctctgtggag aatatagcag gtgtttgcaa cgaccgccac aatgtttttg gtatgatgcc
    13801 ccacccagag cgtgcctcgg acgatgaact gggcaatacc gacggcagac ttattttcga
    13861 gtcaatgata aagtatatca gggagttgag taattaaagc atacgtaaat cgataattgg
    13921 atcgttgggc gggttaccta tcagctcaac gatttttttt gctacctgct cggttgaggc
    13981 aagcattcct ttctgcttgt aatggtgaaa cctctcggat tggggaaagt acgttgggtc
    14041 aactttacga accgagtcct gcatgggggt gtcaactata cctggcgcta atgaaattac
    14101 cctaaccttt gagtcgccta acagtttttg ctcagcgctt agcaccattg aaaacatatc
    14161 gatagcagcc ttggaagcgc aatacccgct ccacgattcc actgcatggc gcgctgctcc
    14221 ggaactgatg ttcacaacgg taattagttt agcagagggt aggtataacg caagtaaacg
    14281 gttagttagt attgcagggc taacggtatt aaccatgaat gttttctcaa gctgttcatt
    14341 atctatttta cctataggtt ttacatcgcc tagtatgcca gcattattta taagtacaac
    14401 gtgtttggca ttgggaaaga aaggtatttc tacctctctt gctgctccct gttggctgaa
    14461 atcaatttga atatgtttat acctatggtg gctaactaca tttctacgtg agtatcctac
    14521 cacataggag ctgtcattgc ttagtacttg gcgagtaagc gccaacccaa ttcccgagct
    14581 agtaccggta atatagaaat aattcatatc ctccttttta gtatcaaact aaacagcttt
    14641 ataaaacgat ttgttttaaa gctgttagta tgaaaagtat ttacttgatg aagtgtcgac
    14701 gaacccagcg ggggaaaatt atgctaccca ggaagaggta aggatagtat gtaaccagtc
    14761 tccacagcaa ggccattgcc acagaaacag gaacaattaa tgctgcatca acagggataa
    14821 attctgaaag gtaacgggta aaaacaaact cggcaaagcc actacctccg ggagttggac
    14881 tcactaacat cattatccac attaccaact ggcgtgcaaa aattagaagg ttgtgctcaa
    14941 tgctaaaaaa tgccataagc aaagcattta caaccaggaa gcgcgatgac catgagagaa
    15001 acgaggcaag aacaaccttt acccagaacg atatgggctt gtttcggtat tccttggagc
    15061 tgtttataat atcggatcct gcgcggttag caccatgctt ccatcgcctt aggaatggta
    15121 acttaaaaat taaaagtaaa agccatttaa gtccacgagg gttaaagaat aaaccgtaaa
    15181 ccaaggttat tacccaggca agttttaatg agtagccaat aatgctgaat gttaaaagct
    15241 ccttggccat gctgtttgtg ttaccaatat cgaataatcc atggtaacct gccagcaaaa
    15301 ccaggagtgg aaacattacc acaaagtaaa gctcatccag gaacgatgtt atcattacca
    15361 gtgctgagct atgcccaaga tttaaaccct ccttattgac ataaacaatt gccacgctgg
    15421 tgccacctat agccgatggt gtgattgccg aagtaaattc ccagagcatt atcaccctaa
    15481 gggccttcca gaatcggaat ttaccatcgc ttaaaatcat tagtcggagg gtgtaaccca
    15541 aatcgcggcc aaccatgaaa agccaagcca gcattagcca caacgctgat ttccatgtaa
    15601 agtttataac gtcaaaagcc ctagggttaa attcattgta tagtaagact cctgttaccc
    15661 ccagcccaag caaaaccggt attagggcaa gttttgcctt tatacgttgt gcagggtttt
    15721 ccttaatatg ggcggtttgc ttatgcatta atttcaaatg caataattaa caaatttaaa
    15781 gttttagggt ttaaatcatg gctaattaaa agctaaattg tttgctgggt taaaaacatg
    15841 aactttaaaa ctcttctgta agttttactt ttttaaactt tactttgttt tagaaatcga
    15901 actgcattgt atttttaatg tatatggtga ttgcaaaaaa taagcgagcc gaggcacatt
    15961 tagccataat acttgcaaac attatgtttg ggcttaacta ctcaatagcc aaaggggtta
    16021 tgcccaatta cctatcgccc atggcattca ctctccttcg ggtggttacg gcctcgtttc
    16081 ttttctggtt gatttcgata tttgacaaga gtgatgaaag aataagcaaa gcagattatc
    16141 cacgatttat tgcatcgggg ttgtttggca tttcattcaa tcagctgcta ttcctcaatg
    16201 ggttaaacta cagtacccca atcgagtcat caatcatatc aaccttgaac cctgcaatgg
    16261 tgatgcttat tgcatacttt gtcctatcgg aacatatcaa ttttgttaaa gtaacaggtt
    16321 tgcttattgg agcatcgggt gcccttttgc ttatcctggg caagggctcc ttggaggttg
    16381 ggcagaacca tacccttggc aatgccatgc ttttcctgaa tacacttttc tatgcttttt
    16441 accttgtggt agtaaagccc cttatgctaa agtataaacc aattacggtt atgaaaggtg
    16501 tttttgtggt tggtttgatt tccctgcttc cttttggtgt ttacgattta gccactacaa
    16561 ggtgggcagt tattccacca atgatttacg cttcaatact ttttgtttta cttggcccaa
    16621 catttttagc atacctcctg aacggatggg gattacggta cgttcaggca agcactgtaa
    16681 gcatatatat ttactctcag cctgttatag cggccttagt tgctgtatcg gtgggaatgg
    16741 atagcctcga tgccaaaaag ataactgctg cagctttggt atttgcaggg gtttacttag
    16801 taagccagcg tcctgacggc tggctggtta gtattttaaa aaggattcag aggaagtgat
    16861 ttcacgtgct attgaatcca gtaggtcaac cccatcaata ataaggtcat ccggaaaatc
    16921 gtaggttggg ctatgtatat cggggtggtc ttcacccgcg ccaagtccga aaattaccga
    16981 tgggcatacc cctgtaaagt gggcaaaatc ctcgctccag cggttgggtt cattgaggta
    17041 tataacctct ttcttttgct ctgaggcaat tttctctaca acattagtaa gctgagggtc
    17101 gcaaactgag gctgggaaat cgtcggtaaa ttcggtttca atggctaagc catgtctggt
    17161 tgcaatttgg gttgatgttt tgagagcgtt atcggaaagt aggtgcatat cggaatcgtc
    17221 aaaggctctg agtgtagcca taataacagc cgaaccggct gaggttccaa aggccacttc
    17281 acctacctta acgtgtataa gggttaccag cacaaagttc ttatacttaa aattggttgg
    17341 caataccagc aggtgctgaa tgatttcagc tgtagccgcc gatggattaa gtcccagttc
    17401 tgggaaggct gcatgggagt ttcgtccggt aagcttaatg atcaagcctt ttgaggctgc
    17461 cgtaaaggtg tatcggccaa gcacaacgct gccctttttg tacttgggta aattatgaag
    17521 tgcaaatgca tagtcgggca tgaggtctag ctcttttagc ctttcaatgg attccttggc
    17581 accggaacca ttctcctctg agggttgata gaacagaatc accttcccct tttctaaagg
    17641 attttcaaaa aaacgaaccg ccaggcctga aacaatggcc atatggccat cgtggccaca
    17701 ggtatgtgaa actccgggag tattggactt gtacttaaaa tcggagagct cagttatggg
    17761 cagggcatcc atatcgcagc ggaatagtac tgtagggcca ggctctttac ccctgtatac
    17821 tacagccatt ccctcaccag caatatttga gtaaagctca tgtggttggt agcggcttag
    17881 aaaaaaacga attcttttcc ccgtagctct ttcttggcca gagagttcag ggttgcgatg
    17941 cagctcgtga cgtagcctga tgagttcttc catacctgca accaaatttc tgtaaaatta
    18001 tcgatttttg gtaaataaat ccaaatccag ccaaataaaa caatcaaaaa aaagatatat
    18061 aattaacaaa aattaaccaa caaaagaata tgtaaataga atactaaatg aacaaagact
    18121 attttttaat gttagtaggg cataatcaac taaaacacac aattatgaaa acaagaaaaa
    18181 atctatttgc tctactagct ttggtaggcc tagcgggaac gctactatta acaagttgcg
    18241 aaaaggatga agaaaaggag atggagatgc ctaaaaacat tgttgaggtt gccgtttcca
    18301 atccacagtt ctcaattctg gttcaagccc tccagaaggc gaacttggtt acaacccttc
    18361 agggaactgg tccttttaca gtatttgctc caaccaacgc cgccttcaac gagctcttca
    18421 gtcagttagg cgtgagtagt atagatgcct taactgctga ccagctaacc ccaattctgc
    18481 tttaccatgt tttatcgggt aaggttgaat ccaatcagct tgcgagtggc tatgtttcaa
    18541 ctcttagccc cggtgcaggt ggattaggtg tttcacttaa ggttgatgca agtatgctca
    18601 aactcaacgg aaatgttggt attaccgctg ccgatatttc agccaccaat ggggttattc
    18661 atgtaattga taaggtttta cttccgccaa cagtggttga tattgccctt gcaaacagca
    18721 gttttacatc tttagtggct gcgctaacca aggccaacct ggttaatgct cttaaggctg
    18781 atggaccgtt tacagttttc gctcctacaa acgatgcatt tagtcaactt ttcactgatt
    18841 taggtgtaag tggtttagat gcgcttaatg ccgaggattt gactccaata cttttatacc
    18901 acgttttagg tactgcagtt aaatccacac agcttcaaac tggctatgtt tcaactttga
    18961 gcgcaggtcc aaatgactct aaagtttcct tacttgtcga tgccgctgct gttaagttga
    19021 acaacaattc taaaattgtt gccaccgatg tggttggtac aaacggtata gttcatgtta
    19081 tcgataaggt tatacttcca ccaacggttg ttgatatagc attggctaat agcagtttca
    19141 gcactcttgt ttcggttcta gtaaaagctg aacttgttga aaccttaaag ggcgagggtc
    19201 catttacagt gtttgctcca accaacgatg ctttcagtgc acttttcact caaattggag
    19261 tatcaggaat cgaccagctt tcaaaggatg atttaacgcc cattctgctc taccacgtag
    19321 tatcaggtaa tgtgagatcg aatcaaattt ctagtggtaa tgtatctaca ttgaatggcg
    19381 atatcaatgt taatgttggt acaaccgtta caattaacga gaacacttcg gttgttctgg
    19441 ttgatgtaca ggctaccaat ggtgttattc atgtaattaa caaggtgctt ttgcctcccg
    19501 caaaataagc ttgcattatt agattagggc gttgagtgtt caacgccctt tttttatttt
    19561 tgtcgcgtaa accttttggg tttataccgt tctaaacctt tgtcaataaa ttgaacttta
    19621 aacaatgaag aataattcca ttgggattta cgctgcaact gtgctggcca tggttttttg
    19681 gtcgctctcc tttatttggt acaaggaggt attccaattc tataaaccta tcactttggt
    19741 ggttggtagg ctatctattt ctgccatttt cctttttatc acggtaggac taataatgag
    19801 gaagttgacc aaaattaaag ctagcgattt gaaatacttt cttttgctag ccttttttga
    19861 acccttcctt tactttatgg gtgagagttt tgggatgacc atggtttcat ctacattagc
    19921 tgctgtaatt atctcaacca ttccactttt cagccccttg ggggagtttt atatttacaa
    19981 gacccgagta acactaatga attttgtggg tatcatggtt tcggttgttg gggttggcat
    20041 ggttatattc catcagggat ttgggaaact tgaggctaac cctgttggta tagcccttat
    20101 gattctggct gtatttgccg cattgggcta ttcacttacc cttaggaagt tgacatcggc
    20161 ctacaatgtt tttacaataa ttacttacca gaatactatt gccgtaattt actttctgcc
    20221 aatatttttc ttgtttgagt ttaaccattt catgagcgtt ggagtaacca tttcctcgct
    20281 aatgccgctt gttaagctag gaatttttgc atcaaccttt gcctttttgc tatttacata
    20341 ctcggttaag catcttgggg taaccaaggc aaactcgttt gccaatgcaa ttcctgtgct
    20401 tacagccatt tttgcctatt tcctgttagg tgaaacttta ggaattgtaa aaatagctgg
    20461 aatagccatt gtagtgcttg gattattcct ttcgcaggta ccaagggaac atgcccggcg
    20521 tgtttttgcc tttataccag tgggtttaat tacccgtgcc cggctaagaa aaaacgagta
    20581 gcattggcta ctcgtcgcca aattgcatca ggtaagcttt aatacattcg ttaaggtcgc
    20641 catcgagtat ggcatttaca tcggaggttt catagcccga ccgtaggtcc ttaacgagtt
    20701 tgtaggggtg gagcacatag ttccggattt gtgagcccca ctcaattttt ttctttttcc
    20761 cttcaatctc ggcctgaatc tcacggcgtt tgcgtagttc caattcgtaa aggtgcgatt
    20821 tcagtagtcg catggcattc tccttattct ggaattgcga cctcgattcg gtattctcaa
    20881 ttacgatgcc tgtagggatg tggtaaaggc ggacgcctgt ttcaacctta tttacgtttt
    20941 ggccaccggc ccccgatgaa cggtatgtgt cccatttaat gtcggctggg ttaatggtta
    21001 tttcaatggt ttcgtcaact gctggcgaaa caaagaccga tgcaaacgat gtgtgccttc
    21061 gggcattgct gtcgaatggg gatatgcgaa ccaggcggtg aacgccattc tcgcccttta
    21121 gaaatccgta agccatttcg cccacaaact ccagtgtaca agattttacg ccggcctcct
    21181 cgccatcctg cacatctaca acttttacct cgtagtcatt ccgctcgccc cagcgcatgt
    21241 acatgcgcat gagcatggcc gcccagtcca tactttcagt tcctccggca ccggagttga
    21301 ttttcatgat tgccccaagc ctatcctctt ccttaccgag catgttcttt agctccaaat
    21361 cctcaatggc ctgcagggtt tgcctgtagt gggagtcaat ttcttcttcg gttgcagcac
    21421 cttccttggc aaagtcaaac ataacctgca aatcctcggt aaggctgttt accttattaa
    21481 agccatctat ccaggatttg attgaagcta ctttcttaag ctgtttctct gcttcatcgg
    21541 gcttatccca aaagtcgggg gcttgtgttt tttgctgttc ctcttcgtat tctatactaa
    21601 gcttgtcgat gtcaaagata cctcctcagc gcatcgcggc gctttgcaag ctcttttacc
    21661 tgttcaatgg ttgtcattcc gtaaaaagat ttttgcaaag atatagattt tatggtagat
    21721 gccctagaat tgaaggcaat catgaaaatt attgaaagta cattctagtt ctattggcat
    21781 gtgaagagct tgtctatttt taagaggctt tcttcctgta tttcggtgaa ccttttgtag
    21841 ccttcgttcg caattgattg ggtaagctgt ggcgattgta ttaatctttc aatcttctgc
    21901 gaaatatcgt aggggtcaac actttcaagg aaaagcagtt gcttgccatc aacaaaggta
    21961 tcctgtaaat tttcctttcc gctggttata acagcacatc gttccgacat ggcttccaga
    22021 ataaccaggg ggaagtactc tagttttgat ggaagtacaa agatacctgc cctactatac
    22081 agttcaaatc ttttgggacc gtaaacaggg cctacaacct caaccgttct gttcaaccca
    22141 tactgtttga ttcgttccag caagttttgc tcttctgttt cggatgggaa tgcccctgca
    22201 agggtgagct taagctgtgg gtatttgctg ctgagtattg caaatgcttc aactagctca
    22261 aaatgtccct tatgtatcag aaaatttgaa aggaatagga tattactcca gtccttttcg
    22321 tgcctttcgt attcgggtgt tttgattatg gagttgggta taacggtaat tttagttttt
    22381 tttaagttca aaggggaaag ctccaacctg gccaggcgag gggttaggtg aacaatttcg
    22441 cagtttttga aaataaagct atagaatcgg tacaggtatg gtttttgact ataaaactgt
    22501 agcattcccg gccggtgaag atgaattatc ttttttgatt ttggtgataa tgccttgcaa
    22561 agcattaaat aaaagctatc cctaatcaac acaagtccaa tgggaaccat tgagaagtaa
    22621 actatatcgg gcttgtactt aatgatttta ccaataattt cgaaaacaac cgttatggcc
    22681 ctaagtattt tacggggttt aaaccgctgt aaatcggaaa gcgattttga gaagttaagg
    22741 ctaatggtaa agcatttgtt gtttggcaat tgcgaagccc tacccattac cagcttattc
    22801 attaaactag caccatgaac cggaggcgga agttgaacaa taaagagtat attactcatc
    22861 taattattct gttcatggaa taataatcga tttaaaggtt agccctttag agctgagatt
    22921 atcgagtacg cggggcagtg cgtagcgcag gttacgctca gccttcaagg aatcatgaaa
    22981 aacaatgatt gacccgggtt tggcatttcg aactacgtac tggtaaaccc ttttgggtga
    23041 gacccttcgg ctgtagtcca tgctaagcac atcccacaaa ataatcttgt attgcgattt
    23101 tagcagcttg aactgtttgg gcgttatacg cccgtagggt gggcggtaga ggttactgtt
    23161 tacaaagctg ttggcgtaaa taatatcctg cacatactga gtggtgtcgg ttccccatcc
    23221 cttgatatgg ctgtaggagt ggtttcccac agcatgtcta tcggccacaa tcatctcaaa
    23281 aatttcaggg tgcatctcaa tgttttttgc caggcaaaaa aaggtagctt ttgcattgta
    23341 cttgcgcagc tgctccagca cccatggggt aactcccggc gttggaccat cgtcgaacgt
    23401 gaggaaaacc ccttcctttt cggaggggaa attccaaatc acattcctga agaacctctg
    23461 aaaaaaacgc gggggactaa gatttatcat aactaccctt caagaaccgt aacgtatgag
    23521 ttgaacttat cagttagctg tttagcgtac tcgcccttct cgtactggtt ggccattcgt
    23581 gttaactcat aaagtattgc aagatttagc tgaagctcgt ttccaagcca atttttttga
    23641 aactcgtcca gatcataaat catgtacgat atggcctcgg aggcttggtt taccatttgc
    23701 tgagagtatt tcagggcatt ttgcatatcg cctgcacggt aatagccttc aaccatggaa
    23761 agggcaaagt agttaagcgg aacattttta ggaggaatta gctccatgca cctgttcagt
    23821 gcagtgatgg ccgaatccat tttgccttca tcaactaaag cattggcaag gcgagcaaaa
    23881 aggttacggt agttagatac aattcgcatc ttgttttcgt ccagatagac cttggggttg
    23941 ttcaagctcc tgaacctata cttttccatg aggttttccc acatctcgcg ggtgttaacc
    24001 gaaccagtcc cccactgccg cgaggcagga ataggtagta cacggtacat cataccctcc
    24061 aggtggaaat acttatccaa acctaaatag gtttcaccgc taacagttgt ggcgtaataa
    24121 atgggacgcc tccattggtt tgtagcaata aggtcaagga ttacctgccc gtccttaagc
    24181 acaacctgct tgttaagtgt ccaggtcatg gtgtcggcaa tgcggtttag gtagttgtcc
    24241 ggcaaatcga ctttgctttt aacctctgat ttatcaacgg ttaggaaaag ctgtttggaa
    24301 gggatgtagt ttatcctttc gttttggtta aatggtgatt gaatcatatt ttcgggtttg
    24361 tcagaaagga agaaatccac cacacggtca aggttcaggg gttcttttat cctatcaatc
    24421 accacaattg catcgcgagc gccggtaaag tattggtcgt atgtcattcg gattggtatg
    24481 gggtcgctct ggtaagcttt acgctgcatc tgttcaatgt accactcgcc gcgcaggtag
    24541 cttaggttag caacacgaac atcggttcta acgccctcca cctcctgtga gtaccatagg
    24601 gggaaagtat cgttatcgcc ataggtgaaa agaatggcgt tgggtttgca gccaataagc
    24661 atgttatacc caaaatcaac tggcacatag ctccctgagc ggtcgtgatc gtcccagttc
    24721 tcgctggcca ttagtacggg tacggccagc gtgcataccg atgctgtaac cacagccgtg
    24781 gctggtgtat cttttatctt tttcagaagt tcataaactg ctaaaacacc taaacctatc
    24841 caaatggtaa aggcgtaaaa gcttcccgca taggcatagt cgcgttcacg tggctggtag
    24901 ggcgtttggt tcaggtaaac aactatagcg attccggtca tcacaaaaag catggtggtt
    24961 acccaaaaat cgtgcttatg cctactaaga tggtacaata aaccggctaa acctaagagc
    25021 agggggagta ggtagtattg gtttcgccct ttattgttac ggtaaatttc aggcagcttt
    25081 tcctgcggcc ctaaacgtgg gttatcgatg aaagggattc cgcttatcca gttacccttg
    25141 gttacctcgc catgcccctg aaggtcgttt tggcggccag caaagttcca cataaagtag
    25201 cggaagtaca taaagccaag ctggtatcgg aagaagaaga gtaagttctc gccaaaggtg
    25261 ggcacgtata tggtttcggt tttgccatcg ggcccaaagc gtttaatggg gttgccatca
    25321 aagttgctcc atttcttgta agcgttaaca tggttttgtt cgttgctcca catacggggg
    25381 aagagcattt tgaactcagg gtcaaactcg tatttatcct tggtgcgaac aaaaacgtac
    25441 ttaccattct ttggcgcata ggtaggctcg ccctcaaccc ttgctatggg ctgtgcattg
    25501 aatacctgac cgtacagcaa aggtctatcg ccatactgtt cacggttaag gtagtatagt
    25561 agtgagaaca tgttatcggg actattctga tccataggcg gattggccga cgagcgaatt
    25621 actattgcag catacgagct gtagccaatc attatcaccg taagccctac aaaaattgta
    25681 ttcagcacca cgcggccttg cttatgtgtt ctataaatac cgtaagcaag cagcgccagt
    25741 acaacggtaa acaggaagaa aacacccgat ttaattggta gtccaaagga atttacaaaa
    25801 agcaactcta cctttgaagc tatcacaaca aatccctgaa ttataccata cattatggta
    25861 attagcagca cacctgaaat taaaagggtg taaaataggc ctttaggcga gtagctgtat
    25921 ttcctgaaat agtaaacaag agcaatggcc ggaattgcca acaggttgag taagtgtatt
    25981 ccaatggaaa tacccattaa aaaggcaata agaatgatcc atcgggtggc atggggctga
    26041 tcggcttgct cttcccactt taggattgcc caaaaaacaa gggctgtaaa gagcgatgat
    26101 gtggcataca cctcaccttc aaccgctgag aaccagaacg tatcggaaaa ggtgtaggca
    26161 agagcgccaa caaaccccga tgaaagtatg agtattactt gggttaaggt gaactcagtt
    26221 gctttgggcg aaacaattcg ccgagccatg taggttatgg tccagaaaag gaacaggatt
    26281 gtgaatgagc tggctagggc cgataggcta ttggcaagta taggaaccca ttgcggggat
    26341 ggtgcaaaca tagtaaagaa acgaaccata agcatgaaaa agggtgcccc cggaggatga
    26401 cctacctcaa gcttattacc gctggctata aactcgctac aatcccaaaa actggcagtg
    26461 ggttcaatgg taagcaggta aaccagtgtg gctatggcaa aagtgaacca tcctattagg
    26521 ttgttatact tcttgaattg acttactgat aagttctcca tcgggcaatt ggttttgtta
    26581 taccttttaa tttcagggtt cagccaccaa aggtatcaat tttctgaatt gggagcgttt
    26641 cggaattaac agaatgttta tttttacatc aaaactaaag attatgactt ctacctatcg
    26701 ttatgtattt aaggggttgt tggccctatt gctatttggc attgtggtgt cctgttcaaa
    26761 ggacgacgaa attgttgacc ccaccagtaa gcccgatcct gaaattgccc agttaaccaa
    26821 aaaaatccat gccgatagca ttgctgctac aattggcatg cttcagggaa tgggtacacg
    26881 ttttgcactg gccgaaaaca ggcgcgatgt agccgttgca ataatgaata agtttaaaag
    26941 ctacggatac gctactgccc gtctcgattc attccagctt aacgtatatt tccgaaatca
    27001 gtactatgaa acctggcagt ataatgtggt ttgtactttg ccaggggttg agttccccga
    27061 tagccttacc attattgggg ggcatcacga ttgtatttta cgaagtactg aacccgaaag
    27121 ccctcttgac gtggctcccg gtgccgacga taacgccagc ggcgttgcgg taacccttga
    27181 aattgccaga gttcttaagc aatcgggttt tgcaccccga acaaccattg tttttgcaac
    27241 ctttgctgcc gaggaacttg gcctctatgg tagccagcat atgaccaacc gtttatccga
    27301 tactttggct aggtgaaatt catgctcaat aacgatatgg tatcgttctg cccggttgcc
    27361 gattcaacgg gttggtacct caatgttatt gattatccca attcggttga ttttaggaag
    27421 catgcacaaa aaatctgttc aacctacacc ctgctcaaaa cggttaacga caatacctac
    27481 cgaaactata gcgatagcta tagcttttac cttaggagct acaaatcgct tttcttcata
    27541 agccagcacg acgaccccaa ctaccacaag ctaaccgact tactctcaaa ccaaaaccca
    27601 cgttttgcgc gcgaggtggc taaactcaac tgcgcgttga tagttgaagc caataaataa
    27661 cctttatatc aaaataatcg tataaatttg aattgatatt aaagcagatt tgtatgaaac
    27721 gtactgtggt tatacttgtt gggctggtta tcatagcttt tgcagcctac attttgctta
    27781 agccccgttt ggcacaaaac agctacaatg aaggtttaaa gctgctcgat tcggccaagt
    27841 atgagcttgc ccttgaaaag tttaacagag ccattaagtt aaaccccaaa cgctacgatt
    27901 acttctttaa gcgcggtgta acccttgccg cattaaataa cgactcgctg gctttggccg
    27961 attttagtac ggctattggt attgattcaa cccattttga gctgtacatt gaacgtggag
    28021 tatcgtaccg taagctaaag cagtattcgc aggccatgac tgacctgaac cgggctatag
    28081 caatgaacga ttccagctca aaggcatact accagcgtgg gttgttaaac gctgcaacca
    28141 tggattttga gcaagccatc aaggactata accgcagcat tgagctcgat ggctcaaacc
    28201 ttgaggcgtt ttacagtcgt ggcgagtcgc tggcaaacct tgccgacttt aagggagcaa
    28261 ttgccgatta cgataagatg attgaagggg ctaacgctac ccctgaggcc tacaagaaga
    28321 ggggaatctt taaactcaaa attcaggatt acaatggggc aattgccgat ttggaggtag
    28381 cccttaagga actccccaag gacgaggaag ccctttatag ccttggtgat gcctttgcca
    28441 atgtgcagcg attcgacgat gccattaagg tactcgataa ggccatagca gttaacccta
    28501 aattaccatt ggcttacgga gtaaagggtg gagcattgct ccgaaagggt gagttcaagc
    28561 aggccgttgt tgctttcaat aaagccattg agcttaagcc cgattacctt aaagcatact
    28621 acggcagggg tgttgcccgt gcgttccaaa aggattacaa gggttcaatt accgatttca
    28681 acaaggtgat ttccattgac cccaattacg ttcaggctta ctacaaccgt gccgtttcaa
    28741 aggcaattat gggcaaccat aaggatgcca tccccgatta tgatgttatc attaaggccg
    28801 accccaagaa tgccgaggca tactataacc gcggtatctc aaaaatgaac gttcaggagt
    28861 tcaagtcggg gtgcgacgat ttccgcaaag ctgtagagct gggctacaaa ccagctgagc
    28921 agatggttaa catctactgt cccaaagaaa ataagtaaat tttcttgcaa gttactatat
    28981 atgtacttat atttgtactg aaaaagtaac ttaaagggga aaagattatg atggccgcaa
    29041 attttacaga gtttagaaca agcctaaaga agtttctgga caatgttgtg aataacaatg
    29101 aaacccttat tattaagaga ggaacgggga aaggcgcagt tatgatgtcg ttagatgagt
    29161 ataactctat aatggaaact cttcacttgt taaattctaa agcaaatgct gacagacttt
    29221 atgagtcaat taaacaaatg aaggaaggga atgtggttcg aaacaaactt atcgaagaat
    29281 gacccgaatt acattttcca aaaattcctg ggaagaatat atatcttggc agaatgagga
    29341 taaaaaaatg ctcaaaaaaa ttaatgagtt aataaaagac attcaaaagc atccttatga
    29401 aggcattgga aaaccagaac ctttaaaata tgatttggct ggtttatggt caagacgtat
    29461 tgaccgagag catcggttag tttatcaagt cattgatcag gaaattctaa tttatagttg
    29521 tagatatcat tatgacagat aaaacactta taataaagtt attaatctca gttttagtta
    29581 attttagtca agatagcatt tcctgacctg gcattttaac aaggagggag gaaaagatat
    29641 ttttagttca atgcatagtt taacggaata acattaaaat tgatggatgt cacccgacca
    29701 ccacaggtcg gggttttact tcacccgtgt aagctttcca tcaaacatca gccgtagcgt
    29761 gtagctgtac tccagcaatc ccgacttaaa atcgtagtat gccttgaaat ttgcgttcag
    29821 cagcaacttc tcccgaaata ccttttggaa ttcaattgaa aaagtaccca ttgtgcgaac
    29881 cgcttggttg aatgtaggat taattgtaga tattgaacca aaaagctctt cgccgcgcgg
    29941 atttacaaac ccatcggagt accagtaacc aatatcgatg gtggtgctat tgtggtatgc
    30001 tctgagttga gggtgcaggc caaagcctga gctgtaatag gaggtaaacg atggggaaag
    30061 gtttttgtag tagtaaagcg atgatgaaaa ttcaaatcgt gttttagcca ggggctggaa
    30121 gctatatgca ggaataactc caaggtttat gtgtgtttga acgggtaggt tggtgctatc
    30181 aatttgcccg ccatggtgga aagccattgc atgaaaggca ttggagaatc cggtattgtt
    30241 gttccggtgg tacagatgtg taaagcctac agtaaactct tcctgaaatg ggctaccagt
    30301 tttgatgtaa cgctcccaat ttatccataa atcggctttg ctctcagtcc ctcgatacaa
    30361 gaactgcaca cccgtttcgg gttggttcag aaataacctt tcgggcttaa atagtggctc
    30421 gggtagccag tggccgtttt tggattccaa tgttcctaca attagggtta ccctgctatt
    30481 tagctgtaaa ctagccgaaa aaacaggtac caaccgttca agcgagtcgg caccggcaag
    30541 gtataaagta tggaaacccg ctgcaatgct gtactgttcc gttttaaatt tgatggccgg
    30601 ctgtatgtaa aagccggggt gggtataccc ctttttgatg ttcccggtaa actccttgtt
    30661 atcaaaaaac gattgtccca aaaggtagag gcctaccccc gagctatccg gttgagcatt
    30721 ggatttgtaa acaatggcaa taaatgttga aataagcgct attggaagca ttatgctttt
    30781 catgattcgg gtcaaatgaa atattttgag aacaaatatc acaaaaaatc caaaacaatc
    30841 cttaatttcg acacaacata tcaacctaaa cctagcatgc tatgaactgg aagcgaaaac
    30901 ttaaggtggc cagcgtagtg gttgttgtag cccttgcagt atatttggct ttgcctcaga
    30961 accattacat tgtgcgggca ctaatttacc agcaggtaaa tatcgacgac tacaaaatat
    31021 tccataaccg taccgttaag gcgggtaccc ctcaaccctg gaggttgcat gaaaagtaca
    31081 actcctatca gcttaccaac gaccaactca aatactttaa cgatttccgc acagtagcct
    31141 tcctggttgt aaaggatacc gccctacttt ttgaatcgta ctgggatgga tacggaaaag
    31201 attcatactc caactcgttt tcaatggcca agagcattgt cagcctactc atgggttgtg
    31261 ccctaagcga tggttatgtg aaaagtatcg atgagccagt tgggaactac ctccctgagt
    31321 ttgctgaggg cgataaggca aaaatcacca taaagcatct gctaaccatg agttcagggc
    31381 taagctggga cgaatcgtat agcacgctat tctcccttac cacgcagggt tactacggaa
    31441 aaaacctgcc caagctggtg ctaaaccaaa cggtggtaaa ggaacccgga aagacctttg
    31501 agtaccgtag cggcgacaca cagctgctat cgctcattat tgaaaaggca actggtaaaa
    31561 cgctggccga ttacgcctcg gaaaagatat ggtcaaagat tggcgccgag cacgatgccc
    31621 tttggtgttt ggaccgcaag gatggcgttg aaaaggcgtt ctgttgcttt aacaccaatg
    31681 cccgcgactt cgcccgcttt ggtcagctgg tgctgaacgg tggccgttgg aatggggaac
    31741 agattgtgcc ggaagaatac cttaacgagt cgcttacacc ggcaagttac ctctttgacc
    31801 cagaaactaa caagaatgtc gatttctacg gttaccagtg gtggatgata aatgttgatg
    31861 gttacaagac ctggtatgct cgcggtttgc tgggacagta tatctttgta attcctagct
    31921 tgaatgccgt ggtggttagg ttgggacacg taaggaacaa caaccgtatc gatggcactc
    31981 ctgaggatgt ttaccagtat atcaggttcg gaattagtat tgttaaaagc attgaaaaca
    32041 atgagtagta acgattttat taaggatagg ttaagggtaa agttgcttgc ttttccggca
    32101 gcagagcttt cagttttcga caaggccaaa aagaagtaca tgggtttgtt taaccccgag
    32161 gagatggagt ttgtgaacga taagcccgat gtgcttatgt ttctgaccgg gggctctgag
    32221 catatagcca ttgagtcggt gcaggagtac cgtttttatc tgctgctcgc ctcgcgcgaa
    32281 gccaatgcat gggctgctgc caccgaggtt aaagcctgga tgaaccagca taacatacgt
    32341 tccgtgctgc tcgatgccga taacccttca accgccgagt acctgagcga gtactacaat
    32401 gcctttgtgg gggtacgcaa gcttcacggt cagcgacttg gtgtggtggg cagctcatcg
    32461 ccgtggcttg tggccagcgg tgttaacccc taccttttac aaagcaagtt gggggttgag
    32521 caggttgata ttagctggga tgatattgtt tttgatgaga tatcgcaggt ttcgccggac
    32581 tttacctcgc tttttggcat ggccgagaac cgggtggagc tcattgagag cggtaagata
    32641 tacgaggggc ttgcctcact tattccgttt tacaggctta acgccatggc cgtggaatgt
    32701 tttccgttgg ttaataaaac cggccatacc gcctgcctgg cattggccaa gctaaactac
    32761 gatggcatac ccacagcctg cgaggccgat atttgcagtg ctgccgcaat gatgctatcg
    32821 tcagaggtat gcaacgccat tccatggatg gccaatgttg cccatgtggg caatggcaaa
    32881 gccattctta cccactgtac tgcgccggtt aaccacctaa cctcgtttaa gctcgatacc
    32941 cactttgaaa ccggcaaggg ttacgccgta agcggccagc ttaagggcga tgaggtaacc
    33001 atctttaggt tcgacagtac gttgaaccac atatttgtaa cgctagccag ggttaccgat
    33061 aagcccaaaa gccggaatgc ctgtcgcacc cagctagagg ttgagcttag cccttcggcc
    33121 tcaaagtatt ttacggaaac tccctttggc aaccaccaca tggttctgcc cgggaactgg
    33181 gtacaacgcc ttaccctggc agcggagctg ctgaatgttg gggtggtgag ttaacgtttc
    33241 tcagctatac gcaggcaggg attttaacca ctgaacttcc tacgaa
//
LOCUS       DAOSAL010000021        32506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_1001_length_32506_cov_46.752920, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000021 DAOSAL010000000
VERSION     DAOSAL010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 32506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32506
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1001_length_32506_cov_46.752920"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="PK049_04665"
     CDS             <1..398
                     /locus_tag="PK049_04665"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOK60886.1"
                     /translation="WHDLLEILLEMKNIRRVNVKVKTLEEKQKEVEFDERFAEQPVVS
                     IPLEAKPEVKEAKPSLDRIKKLHLKLRMEAFQIYRELIKQRTPERITDTLRTFQEQEM
                     LYANGRTKPGKIVTKAKPGQSYHNYGLAP"
     gene            complement(426..1175)
                     /locus_tag="PK049_04670"
     CDS             complement(426..1175)
                     /locus_tag="PK049_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOK60887.1"
                     /translation="MLSKNTIKRVKSLQQKKFRNELGLFIAEGTKLVDELISSNFEIE
                     EIYHTEQYLVTNVNCLVHKVSQSEMERISGLVTPSPVLAVVRFPTYKLDVDPSKELVL
                     ALDTIQDPGNMGTIIRLADWFGIDNIVCSHGCTDAFAPKAIQATMGAIAHVKVHYTDL
                     ITWLSQHTVNCPIIGACMDGENIYKAKKEHAGIIVMGNEGNGISPAVDKLITHRIHIP
                     TFARGRQKVESLNVAMATAIILSEFKGTLHQ"
     gene            1498..3750
                     /locus_tag="PK049_04675"
     CDS             1498..3750
                     /locus_tag="PK049_04675"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOK60888.1"
                     /translation="MLNKVQIKTSSKQVKPDDLLPYVKQKPNKKILGFRFHLRVYSLS
                     NPRKSGWLNSGLRTIGEDPVLFDSMAVSGSSRNIKLYLQSKGFYNAVVTDSVWIHGKK
                     ADVFYTIHPNLPYKVRRVGYFIEDTLIRQLVLADTANRLIKRNDLFDVDILQAERERI
                     ETMLKRNGYFTFSKNFITFTADTNFRNNRVDLNLIIKNPLRADENGKLKPIRFKRYKF
                     RNVFIYPNYDPFRFSSYRDNGELDTICRASVYYVFHQNTGVKLDVIEAFNQLKPNEMY
                     SVDQVNKTQQNLSQIRLFKFVNIELEEIEAKEQTGEIDFSDIDTGSESEEFGYLNCYI
                     QLVPHTLQSYQVELVGTNTSGSLGAEGTLNYQHKNLFKGAEVFDIKFRGLVETAQQKI
                     NLNNTLELGGSMGLTTPKYIGPYTSHVRVDKYAPNTQLTASYSFQRRPEYTRMIAGLQ
                     FGYVWKSSRFMLHTLNPIELNAISIQKISPEFQAQIDTTFLKYSYISQIVTVSSYNLV
                     YSNQNVKKASSYSYIRFNLELSGNALQAISKLSDRPKNSNGAYTFFNTEFSQFVRSDI
                     NLTYHQVADENNSFAYRLYIGVGYPYGNSRALPFEKRFFTGGANGVRAWQARSLGPGS
                     YYQPAERFPNRTADIKLEANFEYRFRMVWKLEGAMFIDCGNIWSMPKLDDRTGATFEW
                     NKFYEQIAIGSGLGIRLNLGFFILRTDFGYKIFDPAINPNEPYKPWVPFNRKFSISDI
                     TFNFGIGYPF"
     gene            3819..4877
                     /locus_tag="PK049_04680"
     CDS             3819..4877
                     /locus_tag="PK049_04680"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007411464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HOK60889.1"
                     /translation="MNYDKIVELLGDNANYLLEHKCNTVPKEMLHLPGPDFMERVVYH
                     SNRSPQVMRSLQAMFYHGRLAGTGYLSILPVDQGIEHSAGASFAPNPIYFDPENIVKL
                     AIEGGANAVASTFGVLASVARKYAHRIPFIVKINHNELLTYPNKYDQIMFGSVDEAWN
                     LGAVAVGATIYFGSEESNRQIIEVAQAFERAHELGMVTVLWCYLRNNGFKKDGVDYHV
                     AADLTSQANHLGVTIQADIIKQKAPENNGGFKAINFGKTHDKVYSQLTTDHPIDLTRY
                     QVVGCYNGRAGLINSGGASSGASDLAEAVYTAVVNKRAGGMGLISGRKAFQRPMDEGI
                     KILNAIQDVYLESKVDIA"
     gene            5011..6372
                     /locus_tag="PK049_04685"
     CDS             5011..6372
                     /locus_tag="PK049_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HOK60890.1"
                     /translation="MPVSNNRDSFGSKFGIIAATAGSAVGLGNIWKFPYITGQNGGGA
                     FLLIYLLFVFLLGIPVMISEFSIGRRGQRNVFGSFKAISPGTPWYFVGIMGVVAAFVI
                     LSFYSAVAGWTLEYIVKSVSVGFGNQTTQQLGDAFQNFLVHPVRPIIWQLVFMALTAY
                     IVIGGIKNGIEKYNKFLMPLLVVFLLILCVRSITLPNASKGLAFLFKPDFSKVTIKTF
                     LFAMGQAFFSLSLGMGALITYASYFRKTDNLGRTALNVSLADTFIAVLAGVVIFPAVF
                     AFNIEPTAGPSLVFITLPEIFSNIPMGNLFGFIFFILLAVAALTSTVSVLEVVVAYFS
                     EELRITRLKATILASVSISVLGVLASLSFGPLSNIRLSGKTIFDMLDFLASNILLPLG
                     GMLIVIFVGWYLKKSTLFDEVSNGGELKAGYFPVYRFVVRFIAPLAIAAIFIYSVFYG
                     SLG"
     gene            6382..7842
                     /locus_tag="PK049_04690"
     CDS             6382..7842
                     /locus_tag="PK049_04690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HOK60891.1"
                     /translation="MDELINSIFEPLVRVLSSFLFWDPFRTFGVEIGFRFPFVVMWLM
                     VWGVYFTFRMGFINIRAFWHSIQITSGRLDKNSKKGEVSHFQALSTALSATVGLGNIA
                     GVAVAISVGGPGATFWMIMAGLLGMSLKFTECTLGQKYRLIDSKGRVSGGPMYYLSQG
                     LAKRNMKTIGNILAVTFSVLVIGASFGGGNMFQANQAFSQMAFIIPWLNGKGAYFGIA
                     MALLVGLVVLGGIKSIASVASRIVPFMVILYISASMVIIFLNISRLGEVFSLILEQAF
                     GLKSIAGGFFGAFIVGFQRGAFSNEAGIGSASIAHSTARTDEPVSEGIVALLEPFIDT
                     VVVCTMTALVINFAGLAGNSSGLQGAQLTSAAFATVLPWFPYLLAIAILLFAFSTMIS
                     WSYYGLKGFNYLFEKRLNGIIPVPEASTKLYYIIFLLCIVVGSASNLWAVMDFSDMMI
                     LGMAFPNILGMIFMAKEVRADLDSYWNRWKTGRLYR"
     gene            7909..8814
                     /locus_tag="PK049_04695"
     CDS             7909..8814
                     /locus_tag="PK049_04695"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOK60892.1"
                     /translation="MKRIWVSVKSFLYFSRGERNGTIILVLLVLLLAVAPYLYKKYFN
                     VVSPPDPMLVQKADSFFLTLQYHEPEKVIEPPSGIEAEMELPRTKKSFLFDPNTVSLD
                     SLMELGLSRKQAEVLVKYRSKGGRFRTPDDIDKIFVIDSATKARLKPLVRIAPLADTI
                     SHDRVIDTKSILVELNGADTLSLLKLKGIGSNYAKRIVGYRNLLGGFYSIDQLVEVYG
                     FTPQLVDQLRPNIYIDTTRIRKININLIKYDDLRIHPYVNDYQAKAIIYYRSQKGTIK
                     TLDELWKNKLLPRDRFEKLKHYLDL"
     gene            8925..9116
                     /gene="rpsU"
                     /locus_tag="PK049_04700"
     CDS             8925..9116
                     /gene="rpsU"
                     /locus_tag="PK049_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOK60893.1"
                     /translation="MIIVPVKEGENIERALKKFKRKFEKTGIMRELRSRQAYVKPSVA
                     RREQIKRAIYIQKLQQQEE"
     gene            9204..10088
                     /locus_tag="PK049_04705"
     CDS             9204..10088
                     /locus_tag="PK049_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOK60894.1"
                     /translation="MDMLASFLKYLDIEKGYSSHTIRSYGDDLHSFFSYSGVNPASTE
                     AIEHISHRTIRAWMSNLVSQGLSTRSVNRKLSSLRTFYKYLLRQGVIANNPMKRVVSP
                     KTSKRLPEFVPEADMAKLNTNDLFGDDFEGLRNRLIITMFYFTGMRLSELVGLTLQSV
                     DVDSMTIKVMGKGSKERIIPIHPDLKDLVVEYLEERNKKFSDTVSFFVTPKGKPVYPK
                     LVYRVVNHYLTLITPLQKRSPHVLRHSFATHLLNKGADLNAIKELLGHSSLLATQVYT
                     HSTFEKLKNNYNQAHPRA"
     gene            10109..10408
                     /gene="raiA"
                     /locus_tag="PK049_04710"
     CDS             10109..10408
                     /gene="raiA"
                     /locus_tag="PK049_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOK60895.1"
                     /translation="MNVKIQSIKFDADRKLLDYVEKKAAKLERYFDNIVETEVYLKLE
                     NSQDMENKVVEFRLKVPGNDLFAQRKGKTFEEATDLCIDVLKGQVKKFKEKLRGV"
     gene            10628..10701
                     /locus_tag="PK049_04715"
     tRNA            10628..10701
                     /locus_tag="PK049_04715"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10662..10664,aa:Thr,seq:tgt)
     gene            10718..10800
                     /locus_tag="PK049_04720"
     tRNA            10718..10800
                     /locus_tag="PK049_04720"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10752..10754,aa:Tyr,seq:gta)
     gene            10841..10913
                     /locus_tag="PK049_04725"
     tRNA            10841..10913
                     /locus_tag="PK049_04725"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10874..10876,aa:Gly,seq:tcc)
     gene            10924..10995
                     /locus_tag="PK049_04730"
     tRNA            10924..10995
                     /locus_tag="PK049_04730"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10956..10958,aa:Thr,seq:ggt)
     gene            11062..12252
                     /gene="tuf"
                     /locus_tag="PK049_04735"
     CDS             11062..12252
                     /gene="tuf"
                     /locus_tag="PK049_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOK60896.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITKILAEKGLSEF
                     RSFDSIDNAPEEKERGITINTAHVEYQTENRHYAHVDCPGHADYVKNMVTGAAQMDGA
                     ILVVAATDGPMPQTREHILLARQVNVPRIVVFLNKCDMVDDAEMLDLVEMEVRELLSF
                     YQFDGDNVPVIRGSALGALNGEAQWVEKVMELMKAVDEYIPIPPRDNEKPFLMPVEDV
                     FSITGRGTVATGRIETGVIHTGDEVEIVGLTNDKPMKSVVTGVEMFRKILDQGEVGDN
                     VGLLLRGIDKKDIKRGMVIAKPGSITPHTKFKAEVYVLKKEEGGRHTPFHNHYRPQFY
                     LRTLDVTGEIMLPEGVEMVMPGDNVTITVELIYPVAINKNLRFAIREGGRTVGAGQVI
                     DIIE"
     gene            12301..12373
                     /locus_tag="PK049_04740"
     tRNA            12301..12373
                     /locus_tag="PK049_04740"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12334..12336,aa:Trp,seq:cca)
     gene            12393..12584
                     /gene="secE"
                     /locus_tag="PK049_04745"
     CDS             12393..12584
                     /gene="secE"
                     /locus_tag="PK049_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOK60897.1"
                     /translation="MKITQYLHDVYTEMVQKVSWPTWKELQSSALIVMIASLLIALVV
                     FVMDFSFERLMEFVYRVLS"
     gene            12613..13161
                     /gene="nusG"
                     /locus_tag="PK049_04750"
     CDS             12613..13161
                     /gene="nusG"
                     /locus_tag="PK049_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOK60898.1"
                     /translation="MEENVKKWYVLRAIGGKEKKVKELVENEIKRLKLDDFISQVLIP
                     TEKVYQIRNGKKISKERIYYPGYVLIEAALVGEIPHILRNIPNVLGFLGDPKTGDPIP
                     LRQAEVNRILGKVDELVDSEEELNVPFYVGETVKVIDGPFNSFSGVIEEVNEEKKKLK
                     VMVKIFGRKTPLELSFMQVEKE"
     gene            13183..13623
                     /gene="rplK"
                     /locus_tag="PK049_04755"
     CDS             13183..13623
                     /gene="rplK"
                     /locus_tag="PK049_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005468463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOK60899.1"
                     /translation="MAKEVAGFIKLQIKGGAANPSPPIGPALGSKGVNIMEFCKQFNA
                     RTQAQAGKVLPVVITYYTDKSFDFVVKTPPVSVTLLEITKQQKGSAEPNRKKVASVTW
                     EQVRQIAQEKMQDLNCFTIESAMKMVAGTARSMGITVTGEFPGN"
     gene            13644..14345
                     /gene="rplA"
                     /locus_tag="PK049_04760"
     CDS             13644..14345
                     /gene="rplA"
                     /locus_tag="PK049_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOK60900.1"
                     /translation="MTKLTKNRKLALSKVEPGKTYKLSEAAALLKEITYTKFDASVDI
                     DIRLGVDPRKANQMVRGVVTLPHGTGKNVRVLVLCTPDKEEAARQAGADYVGLDDYIE
                     KIKGGWTDVDVIITMPSVMAKLGPLGRILGPRGLMPNPKSGTVTMEVEKAVKEVKQGK
                     IDFKVDKFGIVHSSIGKISFEPDKIVDNALEFINTIIKLKPTSAKGTYIKSVYLSTTM
                     SPGIAIDTKSLTASN"
     gene            14372..14899
                     /gene="rplJ"
                     /locus_tag="PK049_04765"
     CDS             14372..14899
                     /gene="rplJ"
                     /locus_tag="PK049_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOK60901.1"
                     /translation="MRREDKNALIDKLTEQINQYPHFYLTDTSELNAEVTHKLRKTCF
                     EKKVKLVVVKNTLLKKALERSGKSTDFSPIFEALKGSTSVMFTETGNVPAKLIKDFRK
                     AHPKPLFKAAYVEESIYVGESQLDVLATLKSKNELIADVIALLQSPAKNVIGSLQSGG
                     QKLSGVIKTLSEREK"
     gene            14935..15315
                     /gene="rplL"
                     /locus_tag="PK049_04770"
     CDS             14935..15315
                     /gene="rplL"
                     /locus_tag="PK049_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOK60902.1"
                     /translation="MADLRKFAEDLVNLSVKEVNELAKILKEEYGIEPAAAAVAVAAA
                     PAAGGEGAAAAEKTQFDVILKSAGGAKLQVVKLVKELTGLGLKEAKDLVDAAPKAVKE
                     GVSKEEAESLKAQLEEAGAEVELK"
     gene            15415..19242
                     /gene="rpoB"
                     /locus_tag="PK049_04775"
     CDS             15415..19242
                     /gene="rpoB"
                     /locus_tag="PK049_04775"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOK60903.1"
                     /translation="MSQNKTNNARINFSKTKSLYEYPDFLDIQLKSFMEFFKLGSTPE
                     ERKNEGLFKVFQENFPITDTRNNFVLEFLDYYVDPPRYSIEECLDRGLTYSVPLKAKL
                     KLYCTDPEHEDFDTVVQDVYLGTIPYMTPRGSFVINGAERVVVSQLHRSPGVFFGQSV
                     HPNGTKLYSARIIPFKGSWIEFATDINNVMYAYIDRKKKLPVTTLLRAIGFETDKDIL
                     QIFDLAEEVKVSKATLTKVVGQRLAARVLKSWIEDFVDEDTGEVVSIERNEVILDRET
                     VIEKDHIDLIIDSGVKSILVHKESQSISDYAIIYNTLQKDPSNSEKEAVVHIYRQLRN
                     SEPPDEATARDVIDKLFFSDKRYDLGDVGRYRINKKLNLNTSSDIKVLTKEDIIEIIK
                     YLIELVNSKAEVDDIDHLSNRRVRTVGEQMAAQFGVGLARMARTIRERMNVRDNEVFT
                     PIDLINSKTLSSVINSFFGTNQLSQFMDQTNPLAEMTHKRRLSALGPGGLTRERAGFE
                     VRDVHYTHYGRLCPIETPEGPNIGLISSLCVYAKINDLGFIETPYRKVENGKVGLTSN
                     DIVYLSAEEEEAKIIAQANAPLTDDGTFVNNRVKARYEGDFPLSESESVDLMDVAPNQ
                     IASIAASLIPFLEHDDANRALMGSNMMRQAVPLLQPETPIVGTGLEGQVVKDSRTQIV
                     AEENGVVEYVDADEIVVRYDKTEEELFVSFDPEIKRYKLPKFKKTNQNTSITLKPIVT
                     KGQRIVKGQVLTEGYATRDGELALGRNLKVAFMPWKGYNFEDAIVISERLLRDDVFTS
                     IHVDEYILEVRDTKRGMEELTADIPNVSEEATKNLDENGMIRIGANVVPGDILIGKIT
                     PKGESDPTPEEKLLRAIFGDKAGDVKDASLKASPSLYGVVIDKMLFSRSIKDGKEAKK
                     AKTNEKALLEKLDNEFNKQVAELKNRLVDKLFSLVNGKTSQGVYDNFDQEIVARGVKF
                     TQKILSDIDYMNVNPNKWTTDKAKNDLIKAVIQNYILKWKEYDAEFKRKKFNATIGDE
                     LPAGIMQLAKVYIAKKRKITVGDKMAGRHGNKGIVAKIVRDEDMPFLEDGTPVDIVLN
                     PLGVPSRMNLGQIYETVLGWAGQKLGIKFSTPIFDGATLEDITKYTDMAGLPRFGRTY
                     LYDGGTGERFDQPATVGVIYMLKLGHMVDDKMHARSIGPYSLITQQPLGGKAQFGGQR
                     FGEMEVWALEAFGASHILQEILTIKSDDVVGRAKAYESIVKGEPMPEPGIPESLNVLL
                     HELRGLGLSVNLD"
     gene            19310..23584
                     /gene="rpoC"
                     /locus_tag="PK049_04780"
     CDS             19310..23584
                     /gene="rpoC"
                     /locus_tag="PK049_04780"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOK60904.1"
                     /translation="MSFRRENKVKSNFSKITISLASPEEILERSSGEVLKPETINYRT
                     YKPERDGLFCERIFGPVKDYECHCGKYKRIRYKGIVCDRCGVEVTEKKVRRERMGHIS
                     LVVPVAHIWYFRSQPNKIGYLLGLPAKKLDAIIYYERYVVVNPGIKAADGINQFDFLT
                     EEEYLDILDTLPKENQYLDDSDPNKFIAAMGAEALYMLLQRINLDELSYELRHKASTE
                     SSQQRKSEALKRLQVVEAFRESAHINKPEWMILKVIPVIPPELRPLVPLDGGRFATSD
                     LNDLYRRVIIRNNRLKRLIEIKAPEVILRNEKRMLQEAVDSLFDNSRKSNAVKTEANR
                     PLKSLSDSLKGKQGRFRQNLLGKRVDYSARSVIVVGPELKMHECGLPKDMAAELYKPF
                     VIRKLIERGIVKTVKSAKKIVDRRDPVVWDILENVMKGHPVLLNRAPTLHRLGIQAFQ
                     PRLIEGKAIQLHPLSCTAFNADFDGDQMAVHLPLGNEAILEAQILMLGSHNILNPANG
                     APITVPSQDMVLGLYYITKPRKGAKGEGTIFYSPEEVIIAYNEKAVDLHAIIKVRLNT
                     LDENGQPKQQIIETTVGRVIFNQYVPAEMGFINELLTKKSLRDISGKVLKKTSVSKTA
                     QFLDDIKDLGYYMAFKGGLSFNIDDVIIPDEKAELVEEGYAQVDEVMSNYNMGLITNN
                     ERYNQIIDIWTHTNAKLTLMLMKKLSEDKQGFNSIYMMLDSGARGSKEQIRQLCGMRG
                     LMAKPQKSGATTNEIIENPILSNFKEGLSVLEYFISTHGARKGLADTALKTADAGYLT
                     RRLVDVSQDVIITESDCGTLRGLVATAIKKNEEVVETLYERILGRTTVHDVFHPLTGE
                     LIIAAGEEITEEIAQIIEESPIEQVEIRSVLTCESRKGVCAKCYGRNLATGRMVQKGE
                     AVGVIAAQSIGEPGTQLTLRTFHVGGTAGSIISDSKIVARFDGRAEIEELKTVQRQDE
                     SGNNYHIVIGRLAEMRIVDSNTGITLSTNNVPYGSKLYINNGDTVKKGQLICEWDPWN
                     AVIISEVSGKVGFDALIKEVTYREESDEQTGYREKVIIESRDKSKNPAVKILSDNEEV
                     IKLYNLPVSAHLTVEDGATIQAGDILAKIPRAVGKAGDITGGLPRAVELFEARNPSNP
                     AIVSEIDGEVSFGKVKRGNREIKVTSKTGEVKNYLVPLSKQILVQENDYVKAGTPLSD
                     GAITPTDILAIKGPTEVQEYIVNEIQEVYRMQGVKINDKHFEVIVRQMMRKVEILDPG
                     DTRFLEGQLVDKWEFIEENDNIFEKKVVVDAGDAAHLRPGMIITARQLRDENSKLKRK
                     DMKLVEARDAVPATSNQVLQGITRAALQTNSFMSAASFQETTKVLNEAAIYGKVDTLE
                     GLKENVICGHLIPAGTGLREFDKLVVYSKDEHQLIEKSKSSTATVK"
     gene            23699..24016
                     /locus_tag="PK049_04785"
     CDS             23699..24016
                     /locus_tag="PK049_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HOK60905.1"
                     /translation="MNDKVNPINIELSDEVAQGIYSNLAIITHSSSEFIIDFVRIMPG
                     MPKAQVKTRVILTPDHAKRLLLALQDNMAKFEANMGTIKLPEGSEGMPPFPMNFGGKG
                     GEA"
     gene            24031..26097
                     /locus_tag="PK049_04790"
     CDS             24031..26097
                     /locus_tag="PK049_04790"
                     /inference="COORDINATES: protein motif:HMM:NF022039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HOK60906.1"
                     /translation="MNRVFTITMAFALAFVSCNKSDSLVVLSPSGKVSITFLINGKGQ
                     PSYSLLFDQKTIVKPSTLGFEFKDMPPLSDNIKVDSFKTRSFSEKWEMPWGDQRWVDN
                     TYNELKVFLSERKSPYRNFAVTFRAFNDGVGFRYEFPEQPGMDSVIILAENTNFNLTD
                     DHTCWWQPGDWDIYEHLYRTTPLCKIDAVNSQSKNPLAQTYIPENAVNTPVTMKTSDG
                     VYLCFHEADVNNYAGATLRVDTVNLSLQTALVGNDSGVKVKAKVPFNSPWRTIIITDK
                     PGDIVTSKLVLNLNEPNRIGDVSWFKPMKYVGIWWEMHLGKSSWDMASGKHGATTANA
                     KRYIDFAARNGIKGLLVEGWNTGWEHWIGFEEREGIFDFVTPYPDYNLKEVVRYAKSK
                     GVSLIMHHETSAAPRTYERQLDSAYRLMQSLGIHSVKTGYVGKIIPKGEYHQGQWMVN
                     HYRKVIETAARYQIAVDAHEPFKGTGEWRTYPNVIARENFRGQEFNAWSVDGGNPPEH
                     LTILPFTAQLAGPMDYTPGIFNIKLKPYKPDNQVNSTLAYQLALYVVIYSPIQMVADL
                     PENYEGHPAFQFIRDVGVDWEQTVVLGAAIGDYIAIAREERYSGRWFIGAITDENPRD
                     LSLKLDFLKPNVKYKATLYLDGPDAHWDKNPTSYRIETREVDSNTVIDLKLAPGGGAA
                     ISLFPF"
     gene            complement(26134..27552)
                     /locus_tag="PK049_04795"
     CDS             complement(26134..27552)
                     /locus_tag="PK049_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007758601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HOK60907.1"
                     /translation="MYSFILAVLGLLLGYFLYGRFVERVFGANSQSQTPAYTMQDGVD
                     YIPMPWWKAFLIQFLNIAGLGPIVGAVLGAAYGPVAFLWIVLGNILAGAVHDYFSGMI
                     SIRMGGLSIPEITGKYLGISVKQFMRVFTLILMILVGAAFISGPAALMASLTPDYLTK
                     NVWVFIILTYYILATLLPIDKIIGRFYPIFGLALIIMAVGITIAIFTGGYLYDIPEIW
                     NNMHNMKANADKFPLFPMLFVTISCGAISGFHSTQSPMMARCLKNEKEGRKVFYGAMV
                     TEGIIALIWAAIGMSFYGGAQGLNSAIAGYNGEATIIVREISNTTLGAIGGILAILGV
                     VAAPITTGDTAFRSARLIVADFLKFSQKSLSNRVLVSLPLFIIGFTLTQVKFDIIWRY
                     MFWSNQVLATVVLWAITVYLAQEKKPYIITLLPALFMTAVVSNYITIAPEGFKLSHWV
                     GYVVGVLTPTVCLYVFAKYLKR"
     gene            complement(27659..29779)
                     /locus_tag="PK049_04800"
     CDS             complement(27659..29779)
                     /locus_tag="PK049_04800"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOK60908.1"
                     /translation="MKSKGLLILTIGLMGTMMACTGKKGPENPFLGEYKTPFGVPPFD
                     KIKVEHYEPAIAEGIKQQLSEIDAITANAEAPTFENTIIPLDYSGELLERVVLTMENI
                     NSANTSDELQEVLKKVSPMLSKHRDDISMNPKLFERVKVVYENMAQNNYTPEQKLLIE
                     KTYKSFVRGGANLDSAKQAELRDINQKLSLLALQFNENLLAENNDYFIVIDKEEDLAG
                     LPESVKAMGAEEAKSRGMEGKWVYTIYKPSMIPFLTYAQNKELRDKLYYGYYMRGNNN
                     NAHDNKNIIKQIVDLRQKKAELLGYPTHAHYVLDVNMAKVPENVYNLLNKLWEPALKR
                     AKAERDDMQKLAKAEGAKYKIGPADWWYYAEKVRKAKYDLDEEMLRPYLSLDAVRSGI
                     FNLTEKLYGLKYVKRTDLPVYHPECEVYEVQEADGKHVGILYLDFFPRESKSVGAWCT
                     EYRSQRIMPDGKFISPVVSIVCNFSKPTGNSPALLSYDEAETFYHEFGHALHALFSKV
                     QYPGLRNVPRDFVELPSQIMEHWAGHKQYLKEYARHYQTGEPMPDELINKIEASGHFN
                     QGFATTEYLAASILDMDYHTLTADKAKIEDVVAFEKASMDKIGLIPEIWPRYRSTYFA
                     HIFSDVMGYSSGYYSYIWAEVLDSDAFEAFVESGDIFNKEIAGKFRTCVLEKGGSKDA
                     MELYKDFRGKEPSIEPLLKNRGLN"
     gene            30033..31379
                     /locus_tag="PK049_04805"
     CDS             30033..31379
                     /locus_tag="PK049_04805"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOK60909.1"
                     /translation="MGAFHAYDIRGIYNKDFNKDDAYKVGFFLVDLLKTNRVLVGRDA
                     RESSDEIFEYLTRGITDAGADVYDLGLATTPMVYYATAKGNFNASVQITASHNPREYN
                     GMKVSRENAEPVGYDTGLKVIEEKIKNGEPIVPASSKGKVIEHNIRAEYIQFLNGFKR
                     DYSNLKIGIDCSNGMAALIIKDILGASPIYLYDELDGAFPNHEANPLIPENVVDLQNL
                     VKANGCDVGIIFDGDADRVMFVDENGRFISPDLMIGVLGHYFLEEKGLKGNVLQDIRT
                     SKAVGEYLSPMGATMHTWRVGRAFAAKKLREIDGIYGGELAGHYYFRDFFYSDSGMLA
                     CILILNVVSKMKAMGISLSQLISRIETYANSGEINFKLERKREAMDAVREHFTSVEQP
                     TASFDFDGYRVEFPDWWFNIRPSNTEPYLRFIAEAKTHKMLEEKVAKVREIIAKFS"
     gene            31389..32306
                     /locus_tag="PK049_04810"
     CDS             31389..32306
                     /locus_tag="PK049_04810"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOK60910.1"
                     /translation="MNMNRLEIKGKHGTYLVNLADSTSVLKPSGEICSTYIGYEKQSG
                     RRVVVKRYHPWVNSASGYFWRIEREAQATSACSGLSSELILLEGIYYLVTDYIEGVSF
                     KELTRWRYHRKLNNYHLIYLASKALDALQKIHDTGFIHCDIKPSNIILTDPKNIANTD
                     VRIIDYGLARKPAEPLHSNEKALAFGLIYSAPEQVFNFWDLIDIQTDIYSMGVTLWAL
                     FSRSEPWVASNPLKTIHVQLTQPLPGNRRVADDLMSILLKSTSKAQLPKPPHYYTRTQ
                     LRALLQQAKSIRYASAVEMLKHIEQLGKR"
     gene            32381..32457
                     /locus_tag="PK049_04815"
     tRNA            32381..32457
                     /locus_tag="PK049_04815"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32415..32417,aa:Pro,seq:ggg)
BASE COUNT         9217 a         6797 c         7331 g         9161 t
ORIGIN      
        1 tctggcacga tttgcttgag atactcctcg aaatgaaaaa catccgcagg gtaaacgtaa
       61 aagtcaaaac gcttgaagaa aaacaaaagg aagttgagtt tgacgaaaga tttgccgaac
      121 agcctgttgt tagcattccc ttagaggcta aacctgaggt taaagaggct aaaccgtcgc
      181 ttgatcgtat taaaaaactt catctcaaac ttcggatgga agcgttccag atttaccggg
      241 aacttataaa gcaacgaaca cctgaaagga tcaccgatac attacgaact tttcaagagc
      301 aggaaatgct ttacgccaat gggcgtacca agccaggcaa aattgtaacc aaggctaaac
      361 ccggtcagtc ttatcacaac tatggcttgg ccccgtaggt ttttaccgtt cttccgcagg
      421 tagatttatt gatgtaatgt ccctttaaat tctgagagta ttatggctgt agccatggct
      481 acattcaacg attctacttt ctggcgaccc ctagcgaatg ttggtatgtg aatacgatgt
      541 gtgattaact tgtcaacagc ggggctgatg ccgttaccct cgttacccat cacaataatg
      601 cctgcgtgtt ctttctttgc cttgtaaata ttttcaccat ccatacatgc cccaataatt
      661 gggcagttaa cagtgtgctg gctaagccag gtaatcagat cggtataatg aaccttaaca
      721 tgggcaatgg cacccatggt tgcctgaatg gcttttggtg caaaggcatc ggtgcatcca
      781 tgggagcaaa caatattgtc aatcccaaac caatcggcta gcctaattat ggtacccatg
      841 ttacccgggt cctgaatggt atccagggca agaaccaact ctttacttgg gtcaacatcc
      901 agtttatatg ttgggaaccg aactacggca agcactgggc ttggtgtaac aagcccgctt
      961 atccgttcca tttcggactg acttactttg tgtaccaagc aattaacatt tgttactaaa
     1021 tattgctcgg tatggtaaat ctcctcaatt tcaaaattgc tactgatcaa ctcatctaca
     1081 agtttagtac cttcggctat aaaaaggcca agctcattcc tgaatttttt ctgttgtagc
     1141 gattttaccc ttttaatggt attttttgat aacatctacc ttaattttag ggcaaaataa
     1201 aacatttttc taatattatc agcattttca aaaattctta gcatatcaca atcgaacatt
     1261 atttttgccc tgattttgat gaatttggtc ataaatcgat aactattgct gtaaaaaata
     1321 gatggcgaat tgctgttata attacatttg tatagttcat aatacctaac tctgattatg
     1381 cctgtgactt tcagttttca gaaaattagt tggcctttaa ggtatatact gtatttttcc
     1441 tcccttttat tagttacagg ttgttccgta accaagcatg tgccggaaaa tatgcacttg
     1501 ctcaacaaag tacaaataaa aacgagttca aagcaggtaa aacccgacga tttattacct
     1561 tatgtaaagc aaaagccaaa taagaagata ttgggattta ggttccacct aagggtttac
     1621 agcctatcaa atccccgaaa atcgggttgg cttaacagtg ggctaagaac cattggtgag
     1681 gatccagtgc ttttcgatag tatggcagtg tcgggtagtt caagaaacat caagctttac
     1741 cttcaaagta aaggtttcta taatgcagtg gtaacggatt cggtttggat tcacgggaaa
     1801 aaggccgatg ttttttatac catccatccc aatttgccct ataaggtgag gagagttggc
     1861 tatttcattg aggatacctt aatccgccag cttgtgttgg ccgacaccgc gaaccgctta
     1921 attaaaagaa acgatttgtt tgatgtggat atactccaag ccgaaaggga gcgaattgaa
     1981 acaatgctaa aacgaaatgg ctacttcact tttagtaaga atttcataac ctttactgcc
     2041 gatactaact tcaggaacaa cagggttgac cttaacctta tcataaaaaa tccactccgg
     2101 gccgatgaga atggaaaact gaaacccata aggtttaagc gatacaagtt tcggaatgtt
     2161 tttatttatc cgaactatga cccattcagg tttagctcgt accgtgataa tggtgagctt
     2221 gatacaatat gtagggctag tgtgtactat gtttttcacc agaacacggg tgtgaagctc
     2281 gatgtaattg aggcatttaa ccagttaaaa ccaaacgaaa tgtattctgt tgaccaagtc
     2341 aacaaaacgc agcagaatct gagtcaaatt aggttattta agtttgttaa tattgaattg
     2401 gaagaaatag aagcaaagga acaaactggt gaaattgatt tctctgatat agatacgggt
     2461 tctgaatcgg aggagtttgg gtatcttaac tgttatattc agctggttcc gcataccctg
     2521 cagagctatc aggtggagtt ggtaggaaca aataccagcg gctcgcttgg cgctgagggt
     2581 acgttaaact atcagcataa aaacctattt aaaggcgcag aggtatttga tattaagttc
     2641 cgaggacttg ttgaaaccgc ccagcaaaaa atcaacctga ataataccct tgagcttgga
     2701 ggttcaatgg ggcttactac cccaaagtac ataggacctt acacttctca tgttagggtc
     2761 gataaatatg ctcctaacac ccagcttact gcctcgtaca gctttcagcg ccgccctgag
     2821 tacacccgca tgattgccgg gttacagttc ggttatgttt ggaaaagttc acgctttatg
     2881 ctacataccc ttaacccaat tgagctaaat gccatttcaa ttcaaaagat tagccccgaa
     2941 tttcaggctc agattgatac cacctttctt aaatacagct acataagcca aatagtgaca
     3001 gtatcaagct acaacctggt ttacagtaac caaaacgtta aaaaggcaag cagctacagc
     3061 tacattcgct ttaatcttga actgtcaggg aacgctcttc aagcaatatc gaagctgagc
     3121 gatagaccaa aaaattcaaa cggggcatat acgtttttca ataccgagtt ctcacagttt
     3181 gttcgaagcg atataaatct tacataccat caggtagccg atgagaataa ttccttcgca
     3241 taccgattat acattggggt tggttatcct tacggtaatt cacgagcgtt accttttgaa
     3301 aagcgattct ttacgggtgg tgccaatggt gtacgagcat ggcaggcacg ctcattaggc
     3361 ccaggctcat actaccaacc tgctgagcgc ttcccaaacc gcactgccga tattaagctg
     3421 gaagcaaatt ttgagtaccg cttccgtatg gtttggaagc tggaaggggc tatgtttatc
     3481 gattgtggca atatatggtc gatgcctaaa cttgacgata ggacaggggc tacctttgaa
     3541 tggaataaat tctatgagca gattgccatt ggtagtggac tggggataag gttgaattta
     3601 ggtttcttta ttttacgaac cgattttggt tataaaattt tcgatccggc aataaatcca
     3661 aatgagccat acaagccctg ggttccgttt aatcgaaagt ttagcatcag cgatataacc
     3721 tttaattttg gtattggata tcccttttag ctttactcta cttgacttaa accaatattt
     3781 ttcgtaggtt tgtatagaac cgataaaatt ctatacctat gaactacgat aaaatagttg
     3841 agctgcttgg cgataacgcc aattatcttc ttgaacataa gtgcaataca gtaccaaagg
     3901 agatgcttca tcttcctggg cccgatttca tggagcgtgt ggtttaccat agcaaccgat
     3961 cgccccaggt aatgcgaagc ttacaggcta tgttctatca tggacggttg gctggtacag
     4021 ggtatctgtc cattttgcct gttgatcagg gaattgaaca tagtgcaggt gcatcgttcg
     4081 ccccaaatcc catttacttt gatcctgaaa acattgttaa gttggccatt gagggtggtg
     4141 ctaatgcggt tgcttcaaca tttggggtgc tggcatcggt agcccgtaaa tatgcccacc
     4201 gcataccttt tattgttaag attaaccata acgagctgct tacctatccc aataagtacg
     4261 atcagattat gtttggatcg gttgatgagg catggaacct aggtgcagta gcagttgggg
     4321 ctacaatata ttttggttcc gaggagagca atcggcaaat cattgaagtt gcccaggcgt
     4381 ttgagcgtgc ccatgagttg ggaatggtta ccgttctttg gtgctacctg cgaaacaatg
     4441 gattcaaaaa agatggcgtg gactaccacg tagctgccga cttaaccagc caggcaaacc
     4501 atttgggggt taccattcag gccgatatta ttaaacagaa agctccagag aataatggtg
     4561 gctttaaagc tataaacttc ggaaaaaccc acgataaggt ttactcacag ctaactaccg
     4621 atcatcccat tgatttgacc cgctaccagg tagtaggttg ctataacgga cgagccggtt
     4681 taattaactc gggtggtgcc tcctcgggag caagcgattt ggccgaggct gtttataccg
     4741 ctgtagttaa taaacgtgca ggtggaatgg gactcattag cggtcgcaaa gcttttcaac
     4801 gccctatgga tgagggtatt aaaattctga atgccataca ggatgtttac cttgaaagta
     4861 aggtcgacat agcttaacaa atgctttttc aaaacattgc ccattctgaa gtccgggatg
     4921 ggcttttttt acgctaacac ttgttttagt gtccattttt ttccaaattg gtgcgccaaa
     4981 atttataacc taaacctaaa ctctaaacct atgccagttt ctaataatcg cgatagcttt
     5041 ggaagcaaat ttggtattat tgcagccact gccggctcgg cagtaggatt aggaaacatt
     5101 tggaagttcc catatataac agggcagaat ggaggaggag cttttttgct tatctatcta
     5161 ctgtttgtgt tcctattggg aattcctgtt atgatatctg aattttcaat tggcaggcgt
     5221 ggtcagcgca atgtatttgg ttcatttaag gcaatttcgc ctggcactcc atggtatttt
     5281 gtgggtataa tgggggtggt ggccgccttc gtaatcttat cgttttatag tgctgtggct
     5341 ggttggacac tggagtatat agtaaaatca gtatctgttg gatttggtaa tcaaacaacg
     5401 cagcaactgg gtgatgcttt tcaaaacttt ttggttcatc ctgttcgtcc cattatctgg
     5461 cagttagtat ttatggcact tactgcatat attgtaatag gaggaataaa aaatggtatt
     5521 gagaaatata acaagttctt aatgccattg ctagtagttt ttcttttaat actatgtgta
     5581 cgatctatta ccttacccaa tgcatcaaaa ggattggcgt tcctgtttaa gcccgacttt
     5641 tccaaggtaa ccataaagac atttctattt gccatggggc aggctttttt ctcacttagt
     5701 cttggaatgg gtgctttaat tacttatgct tcatacttcc gaaaaactga caatttaggt
     5761 agaacggcat taaacgtatc attagccgat acatttattg cagttttagc aggagtagtg
     5821 atatttcctg ctgtatttgc ttttaacatt gaacctactg caggtccaag tttggttttt
     5881 ataaccctac ccgagatttt tagcaacata ccaatgggga atctttttgg gtttattttt
     5941 ttcattttac ttgctgtagc tgcgttaact tcaacggtat cggtgcttga agtggttgtg
     6001 gcgtatttct cggaggagtt gcgtatcact cgccttaagg caaccatttt agcttcggtt
     6061 tccatatcgg ttttgggtgt tttggcatca ctgtcgtttg ggcctttgag caatatcagg
     6121 ctctcaggca aaaccatttt cgatatgctc gattttctgg cctctaacat cttacttcca
     6181 ctcggaggaa tgctaatagt gatttttgtt gggtggtatc taaaaaaatc aacattattc
     6241 gatgaggttt caaatggagg tgaactaaag gcaggttact tccctgtata ccgatttgta
     6301 gttcgtttta ttgcaccgtt agccatagct gccattttta tctattcagt attttacggt
     6361 tcacttggat agactaaggg aatggatgag ctaattaatt ccatatttga gccactggtc
     6421 agggtactat cgagttttct gttctgggac ccctttagaa cttttggggt tgaaataggt
     6481 ttccggtttc cttttgttgt aatgtggcta atggtttggg gagtttattt tactttccgc
     6541 atgggattta tcaacatccg tgctttttgg cattcaatcc aaatcacttc gggtaggctc
     6601 gataagaata gcaaaaaggg agaggtatcc cattttcaag ccttatcaac cgctctttca
     6661 gcaactgttg gtttgggaaa tattgcgggt gttgctgttg ccataagcgt tggggggccc
     6721 ggggcaacat tctggatgat aatggctggg ctgctgggca tgtcgcttaa gtttaccgag
     6781 tgcacgctag ggcaaaagta tagattgatt gactcaaagg gtagggtatc gggagggccc
     6841 atgtactact tgagtcaagg gcttgccaag cgtaacatga aaactatagg taatatactg
     6901 gcagtaacct tttcggtttt agtaattggt gcatcgtttg gcggtggaaa tatgtttcag
     6961 gccaatcagg cattttcgca aatggctttc atcataccct ggttaaatgg taagggagcc
     7021 tattttggta ttgccatggc cttgctggtg ggtttagtag tcctgggtgg aataaaaagc
     7081 attgccagtg ttgcctcgcg gatagttcct ttcatggtta tcctctacat ttcagcatcc
     7141 atggtaatta tctttctaaa catttcacgg ttgggtgagg tttttagtct gatcttagag
     7201 caggcatttg gactcaaatc cattgctggt ggtttttttg gtgcgtttat tgttgggttt
     7261 caacggggag cattttccaa cgaggcaggg ataggttccg cttccattgc gcactcaact
     7321 gcccgaaccg atgagcctgt tagtgagggc attgtagctt tgcttgaacc attcatcgat
     7381 actgtagtgg tatgcaccat gaccgcttta gtaataaatt ttgctggttt agctggtaat
     7441 agttctggtt tacagggagc ccagctaact tcagccgcat ttgccactgt attaccttgg
     7501 ttcccatatc tgctggctat tgctatctta ctattcgctt tttcaaccat gatcagctgg
     7561 tcatactatg gcctcaaagg ctttaactac ctttttgaga aacgtttgaa cgggattatt
     7621 cctgtacctg aggcatcaac caagctatac tacatcattt ttttactttg tattgttgtg
     7681 ggtagcgctt cgaacctttg ggctgtcatg gatttttccg atatgatgat cttagggatg
     7741 gctttcccaa atattcttgg aatgattttt atggctaaag aggtgcgggc cgatttggat
     7801 agttactgga atagatggaa aacagggaga ttgtataggt aacgtaaacc tattgtttct
     7861 tttatcattt ttcacgacat ttggtattag tagaactggt tgctaacaat gaagcggatt
     7921 tgggttagcg ttaaaagctt cttatacttt tcaaggggcg aacgaaatgg aaccattatt
     7981 ctggttctat tagttttgct cttggctgtg gctccgtacc tgtataaaaa gtatttcaat
     8041 gttgtttccc ctcccgatcc catgcttgtt caaaaagccg atagtttctt tttaactctc
     8101 cagtaccatg agcccgagaa agttattgag ccaccttcgg gtattgaggc cgagatggaa
     8161 ctcccccgaa ctaaaaaatc atttttattt gatcccaaca ccgtaagcct cgacagttta
     8221 atggaattgg ggttaagccg aaagcaagct gaggtacttg ttaagtatag gtctaaaggg
     8281 gggcgtttca ggactcctga cgatatcgat aaaatttttg ttatcgactc ggctacaaag
     8341 gcaaggctaa agccgctggt acgtatagca ccacttgccg atactattag ccatgatagg
     8401 gttattgata ccaagtccat tcttgtggag ctcaatggtg ccgataccct ctcattacta
     8461 aaacttaaag gtataggaag taactacgca aagcggattg tgggttacag gaacctactc
     8521 ggtggatttt atagtattga tcagcttgtc gaggtttatg gatttactcc gcaactggtt
     8581 gaccagctaa ggccaaacat atatatcgac actacccgta ttcgaaaaat caacataaat
     8641 ctaattaagt acgatgacct gagaatccat ccctatgtga acgattatca ggctaaagca
     8701 attatttact accgctcaca aaaaggtacc attaaaacac ttgatgagct atggaagaac
     8761 aagcttttac ctcgcgaccg gttcgaaaag ctaaaacatt accttgattt atagtaaaat
     8821 tgatacttca caaacaattg gaataatttc cacaaaaaaa gactaaaata ttttgtatta
     8881 aaatcaaaac attataattt tgcagctcat aaaattcgat aaacatgatt atagtaccag
     8941 ttaaagaagg cgaaaatatt gaaagagctc taaaaaaatt caagcgtaag tttgaaaaaa
     9001 ccggtattat gagggagtta cgttcccgtc aagcttacgt taaaccttcg gttgctcgta
     9061 gggagcagat caaaagagcc atttacattc agaagctaca gcagcaggaa gagtagatgc
     9121 tgatgttcag ttgaatgcat aatcaggcta ttttgcctga tttttttttg ctttttagtt
     9181 tgaaaaaaac tatattgcat tcaatggata tgctggcctc atttcttaaa taccttgata
     9241 tagagaaggg ttactcatca cacaccatac gctcgtatgg cgatgatctg cattccttct
     9301 tcagctattc aggagtcaat cccgcaagta ccgaagctat tgaacacatt agccatagga
     9361 ccatcagggc atggatgtcg aacttagtta gccaaggcct atcaacccgt tcggttaacc
     9421 gtaaactttc atcgcttcgc acattctata agtacctatt acgccaaggc gtaattgcta
     9481 acaatcccat gaaacgtgta gtttcgccca aaaccagtaa gcgcttacct gaatttgtac
     9541 ctgaagccga catggcaaaa cttaatacca acgacctgtt tggcgatgat tttgagggct
     9601 tacgtaaccg gctaataata accatgttct attttaccgg tatgcgccta tcggagctag
     9661 ttgggcttac cctccaatcg gtcgatgtgg attcaatgac cattaaggta atggggaagg
     9721 gtagcaagga acgtatcatt cccattcatc cggatcttaa agatttggtt gtggagtacc
     9781 ttgaggaacg aaacaaaaaa ttttccgata ctgttagttt ttttgttacg ccaaaaggta
     9841 aaccggttta cccaaaactc gtatacagag tagtaaacca ctacttaacc ttgattacac
     9901 cacttcaaaa aaggagcccc cacgtgctaa ggcacagctt tgcaacccat ttgctaaata
     9961 agggtgccga tttgaatgcc atcaaagagc tgcttggcca ctcaagctta ctggctaccc
    10021 aggtgtacac ccacagcaca ttcgaaaaac ttaaaaacaa ttataaccag gctcatccac
    10081 gagcctaaaa ccaaaaggag gatttattat gaacgttaaa attcaatcca tcaagtttga
    10141 tgctgaccgg aagcttcttg attacgttga gaagaaagct gcaaaacttg agcgctattt
    10201 tgacaacatc gtggaaaccg aggtgtacct aaagttggaa aatagccagg acatggaaaa
    10261 caaggttgtt gaattccgcc ttaaggtgcc tggcaacgat ttgtttgccc aacgtaaagg
    10321 gaaaaccttt gaagaggcta ctgacctatg tattgatgtg ctgaagggtc aagttaaaaa
    10381 gtttaaggaa aagctaagag gggtatagca aacccaaaaa atagactaaa aacaaaacag
    10441 atgaagcccg aaagggcttc attttttgct gtaacaggtc aaaacagaca gatggattat
    10501 gaaaattgta ctaaaaaagg ttacataaaa agataaaaat ttttgcagat aattaaaatt
    10561 gttatacatt tgcaatccga tttcaggagg taaacgttcc ttaaaatagt gcaaaaacac
    10621 tttgattgcc gatgtagctc agttggccag agcagctgat ttgtaatcag ctggtcgggg
    10681 gttcgaatcc ctccatcggc tcaaagcaaa acaggttggg gagataccaa agtggccaac
    10741 tggggcagac tgtaaatctg ctggcttatg ccttcgtagg ttcgaatcct gctctcccca
    10801 caaaacaggg ttgacaccag tcctgcaggg ttggtggcat gcgggagtag ctcagtcggt
    10861 agagcatcag ccttccaagc tgagggtcgc gggttcgagt ctcgtctccc gctctacctg
    10921 taagccgatg tagctcagag gtagagcact tccttggtaa ggaaggggtc acgagttcaa
    10981 ttctcgtcat cggctcaatt ttttgtgttg tatgagacaa tcaagttttt aatactaatt
    11041 ttaaattaat accagaaaac tatggctaaa gaaaaatttg ataggtcgaa accgcacgtg
    11101 aacatcggta ccattggcca cgttgaccat ggtaagacta cccttaccgc tgctattact
    11161 aaaatattag ctgaaaaggg tctttccgaa ttccgttcat tcgactctat cgacaacgct
    11221 cctgaagaaa aagagcgcgg tattaccatt aacaccgctc acgttgagta tcaaactgag
    11281 aaccgtcact acgctcacgt tgactgtcct ggtcacgctg actatgttaa gaacatggtt
    11341 actggtgctg cccagatgga cggcgctatc cttgttgtag ctgctactga tggtcctatg
    11401 cctcaaaccc gtgagcacat ccttctcgct cgtcaggtaa acgttcctag gatcgttgtg
    11461 ttcctgaaca agtgcgacat ggttgacgat gctgaaatgc ttgacctcgt tgaaatggaa
    11521 gtacgtgagc tcttgagctt ctatcagttc gatggcgata acgtacccgt tatccgtggt
    11581 tctgctcttg gtgccctaaa cggtgaggct cagtgggttg aaaaggtaat ggaactcatg
    11641 aaagctgttg atgagtacat tcccattcct ccacgtgaca acgaaaagcc attcctaatg
    11701 cctgttgagg acgtattctc aatcactggt cgtggtactg ttgctaccgg tagaattgaa
    11761 actggcgtta tccacactgg cgacgaagtt gaaatcgtag gtttaaccaa cgacaagcct
    11821 atgaagtcag tagttaccgg tgttgaaatg ttccgtaaga tccttgatca gggtgaagtt
    11881 ggcgacaacg taggtctact actccgtggt attgacaaga aggatatcaa gcgtggtatg
    11941 gttattgcta aaccaggctc aattactcct cataccaagt tcaaagctga ggtttacgtg
    12001 ttgaagaaag aagaaggtgg ccgtcacact cctttccaca accactaccg tcctcagttc
    12061 tacctacgta ccctcgacgt aaccggtgaa attatgcttc ccgaaggcgt tgaaatggta
    12121 atgcctggcg ataacgtaac tatcaccgta gaacttatct acccagttgc tatcaacaag
    12181 aacctacgct ttgctatccg cgaaggtggt aggactgttg gtgccggtca ggtaattgac
    12241 attattgagt aattcaataa aaccccgaac gggttcaacg gaatccgttc ggtttcacac
    12301 acgggtgtag ctcaattggt agagtagtgg tctccaaaac cattggctgg gagttcgagt
    12361 ctctccaccc gtgctaaaca caaactgttg acatgaaaat tacacagtac ttgcacgacg
    12421 tttacaccga gatggtgcaa aaagtttcgt ggccaacatg gaaagaactt cagtccagtg
    12481 cattaattgt tatgattgct tcccttttaa tcgcactggt tgtcttcgtt atggacttct
    12541 cattcgaaag attgatggag tttgtttacc gtgtacttag ctaacaatag aacggcctaa
    12601 gaaggagctg taatggaaga gaatgttaaa aaatggtatg ttcttagggc cattggtggt
    12661 aaggagaaga aggtaaagga gctggtggaa aacgaaatta aacgtttgaa gctcgacgac
    12721 tttatctctc aggttcttat tcctactgag aaagtttacc agattcgaaa tggcaaaaag
    12781 atcagcaagg aaaggattta ctaccctggc tacgttctta ttgaagctgc actggtaggt
    12841 gaaattcctc atatcctacg aaacattccc aatgtccttg gttttttagg tgacccaaaa
    12901 accggcgatc caatccctct acgccaagct gaggttaacc gaatacttgg caaggttgat
    12961 gagctggtgg acagcgagga agagttgaat gttccattct acgtaggtga aacagtaaaa
    13021 gtaattgatg gaccattcaa ttccttttct ggagtaattg aggaggttaa cgaagagaag
    13081 aagaagctga aggttatggt gaaaatcttc ggccgtaaaa ctccactgga gttaagtttt
    13141 atgcaagttg aaaaggagta ataattaaag agcaaacgca ctatggctaa agaagtagct
    13201 ggatttatta aactccagat taaaggtggt gctgctaacc catcaccacc cattggtcct
    13261 gctcttggct ccaagggggt aaatattatg gagttctgca agcaattcaa tgccagaact
    13321 caagcacagg caggcaaggt tctacctgtg gttataacat attataccga caaatcgttc
    13381 gattttgtcg taaaaacccc accggtatcc gtaactttgc ttgagataac caagcaacaa
    13441 aagggatcgg ctgagcctaa caggaaaaag gttgcctctg taacctggga gcaagttcga
    13501 caaattgccc aggaaaagat gcaggatctg aactgtttta ccattgaatc agcaatgaag
    13561 atggtggcag gtacagcccg cagcatgggt atcactgtta ccggagagtt tccggggaat
    13621 taacatttaa cctcatttgc gcaatgacta aactgacaaa aaacaggaag cttgctctgt
    13681 cgaaagtaga gcccggtaag acttacaagc tatcggaagc agccgcactg ttgaaggaaa
    13741 ttacatacac caagttcgat gcatcggttg atattgatat tcgcctaggg gttgaccccc
    13801 gtaaggccaa ccagatggtt cgtggtgtgg taacccttcc gcatggtacc ggtaagaacg
    13861 ttcgcgttct ggtactctgt actcccgata aggaggaggc tgctcgccag gctggcgccg
    13921 actacgtagg ccttgacgat tacatcgaga agatcaaggg cggatggacc gatgtggatg
    13981 ttattattac catgccctcg gttatggcta agctaggtcc tttaggacgt attcttggcc
    14041 cccgcggttt gatgccaaac cccaagagtg gtacggttac catggaagtt gaaaaggctg
    14101 ttaaggaggt aaagcagggt aagattgact ttaaggttga caagtttggt atagtccact
    14161 catcaatcgg aaaaatttca ttcgagcctg acaagattgt tgataatgcg cttgagttta
    14221 tcaataccat cattaagctc aaacctacgt cggctaaagg tacctacatt aagagtgtat
    14281 acctttccac taccatgagc cccggtattg ctattgacac caagtcgctc acggcttcga
    14341 attaataacg tttacacaga cccaatttgt tatgagaagg gaagataaaa acgcactgat
    14401 tgataagctg accgagcaaa ttaaccagta cccccatttt taccttaccg atacctcgga
    14461 acttaacgca gaggtaactc acaagttacg taagacctgc tttgagaaaa aggtaaagct
    14521 ggtagtggtt aagaacacat tgctgaagaa agcccttgag agaagcggca aatcaactga
    14581 tttctcgcca atctttgaag cattgaaagg ctcaacctca gtaatgttta ctgaaacagg
    14641 taatgttccg gctaagctta tcaaggattt tcgcaaggct catccaaagc ctctatttaa
    14701 ggctgcctat gttgaagaat cgatatatgt tggcgagagt caacttgatg tgctggcaac
    14761 cctgaagagc aagaacgagc ttattgccga tgttattgca ctactccagt cgcccgcaaa
    14821 gaacgttatc ggttcgctac aatctggtgg tcaaaaatta tcgggtgtta tcaaaacact
    14881 ctctgaaagg gaaaagtaaa aattattttg agaacaaatt aaacacttac taaaatggca
    14941 gatcttagga aatttgcaga agatttggtt aacctctcag ttaaagaagt taacgaattg
    15001 gctaaaatac tcaaggaaga gtatggtatt gaacctgctg ctgcagctgt agctgttgct
    15061 gctgctcccg ctgctggtgg cgaaggcgct gctgccgctg aaaaaaccca gtttgacgtt
    15121 attcttaagt cagctggcgg tgctaaattg caggttgtta agcttgtaaa ggaacttacc
    15181 ggtcttggtc taaaggaagc taaggatcta gttgatgctg ctcctaaggc cgttaaggaa
    15241 ggtgtttcta aagaagaagc tgaatcatta aaagcacaac ttgaagaggc aggagcagaa
    15301 gttgaactta aatagtccta acctatagtg gttctaaggt ttagaatcta tcccgataga
    15361 ttctaagcct ttttcttgtt atgtttattt tatagttcaa ccaattcgtt cgcaatgtct
    15421 caaaataaaa ctaacaacgc acgtatcaat ttctccaaaa caaagagcct atacgagtat
    15481 cccgattttc tggatattca gctaaagtcg tttatggagt tcttcaagct gggttcaact
    15541 cccgaagagc gtaagaacga aggcctgttc aaagtgtttc aggagaattt tccaattacc
    15601 gataccagga acaattttgt actcgaattt ctcgattact acgttgatcc tccccgctat
    15661 tccattgagg aatgtctgga tagagggtta acctacagcg taccactaaa ggcaaagtta
    15721 aagctatact gtaccgatcc cgaacatgag gattttgata cggtagttca ggatgtatac
    15781 ctgggaacaa ttccctacat gacaccccgc ggtagttttg taattaatgg agctgaacga
    15841 gtagttgttt cgcagcttca tcgctcgccc ggtgtgttct ttggccaaag cgtacatcct
    15901 aacggtacca agctctactc cgcacgtatt atccccttca agggatcgtg gattgagttt
    15961 gccaccgaca tcaataacgt gatgtatgca tacatcgatc gtaaaaagaa attgcccgtt
    16021 actacacttt tgcgcgccat tggatttgag accgataagg atatcctgca gatattcgac
    16081 ttagcagaag aggtaaaggt ttcaaaagcc actctcacca aggtagtggg gcaacgtttg
    16141 gccgctcgcg ttctcaagag ttggattgag gactttgttg atgaagatac cggtgaggtg
    16201 gtttcaattg agcgtaacga ggttatcctt gaccgcgaaa ccgtcattga aaaggaccac
    16261 atcgatttaa ttatcgactc cggtgttaag tcaattcttg ttcataaaga atcacagagc
    16321 atttccgatt atgccataat ctataacacg ctgcaaaaag accccagtaa ctctgagaag
    16381 gaggctgtgg tacacattta caggcagctc cgtaactccg aaccacccga tgaggctacc
    16441 gcccgcgacg ttatcgataa gctattcttc tccgataaac gttacgactt gggtgatgta
    16501 ggacgttacc gtatcaacaa aaagcttaac ctgaacacct cgtccgacat taaggtgtta
    16561 accaaggaag atatcattga gattattaag tatctcattg agctggttaa ctcaaaggct
    16621 gaggttgatg atattgacca cctaagtaac cgtagggtgc gaaccgtggg tgaacagatg
    16681 gctgctcagt ttggcgttgg tttagcccgc atggccagaa ccatccgtga gcgtatgaac
    16741 gttcgcgata acgaggtgtt tactcctatc gatctgatta actccaaaac actctcgtcg
    16801 gttatcaact cgttcttcgg aacaaaccag ctttcgcagt tcatggatca gaccaacccg
    16861 ctggccgaaa tgacccacaa gcgtcgttta tcggctttgg gacctggtgg tcttactcgc
    16921 gaacgagctg gtttcgaggt tcgcgacgtt cactatacac attacggacg cctttgcccc
    16981 attgaaaccc ctgaagggcc taacattggt cttatctcat ccctttgcgt ttatgcaaag
    17041 atcaacgacc ttgggtttat tgaaacccct taccgcaagg ttgagaatgg taaagttggc
    17101 ctgactagta acgatattgt ttacctgagc gccgaggaag aggaagcaaa aattattgca
    17161 caggcaaacg ctccccttac cgatgacggt acttttgtaa acaacagggt gaaagctcgc
    17221 tacgagggcg atttcccgtt atccgaaagt gaatcggttg acctaatgga cgttgctcca
    17281 aaccagattg cgtcaatagc tgcatcgcta attccgttcc ttgagcacga cgacgctaac
    17341 cgtgcgctca tgggatcgaa catgatgcga caagctgtac cccttctcca gcccgaaacc
    17401 ccaattgttg gtacaggctt agagggtcag gttgttaagg atagccgaac tcagattgta
    17461 gctgaggaga atggtgttgt tgagtatgtt gatgccgacg aaatagttgt tcgttacgat
    17521 aaaaccgaag aggaactctt tgtaagtttc gatcccgaaa ttaaacgcta caagctacct
    17581 aagttcaaaa aaaccaacca gaacacctcc attacgctta agcccattgt tacaaaggga
    17641 caacgtattg taaaaggaca agttctgacc gaagggtatg caaccagaga tggagagttg
    17701 gccttgggta gaaaccttaa ggttgcattc atgccttgga aaggctacaa ctttgaggat
    17761 gctattgtaa tcagtgagcg tttactccgc gacgatgtgt ttacatcaat tcatgttgat
    17821 gagtacatcc ttgaggttcg cgataccaag cgaggtatgg aagagcttac cgccgatatt
    17881 cctaacgttt ccgaagaggc tacaaaaaac ctcgacgaaa acggtatgat tagaattggc
    17941 gctaatgttg ttccgggtga tatccttatt ggtaaaatta ctcctaaagg agaatcggac
    18001 ccaacccctg aagagaaact cttaagggct atcttcggcg ataaggctgg tgatgttaag
    18061 gatgcctcac taaaggcttc accttcgctt tacggggtgg ttattgataa gatgttattc
    18121 tcacgttcca ttaaggatgg taaggaggct aaaaaggcca agactaatga gaaggctctg
    18181 cttgagaaac tcgacaatga gtttaataaa caggttgctg aacttaagaa ccgtttagtt
    18241 gataagcttt tctcactagt taacggtaag accagccagg gtgtttacga taatttcgat
    18301 caggaaatag ttgctcgtgg tgttaagttc acccaaaaaa tcctgtcgga cattgattac
    18361 atgaacgtaa accccaacaa gtggactacc gataaggcta agaacgacct gattaaagca
    18421 gtaatacaga attacatcct taagtggaag gagtacgatg ctgaattcaa gcgtaagaag
    18481 ttcaacgcaa caattggcga tgagcttcct gctggaataa tgcagctggc caaggtttac
    18541 atcgccaaga aacgtaaaat caccgtaggt gataaaatgg ctggtcgaca cggtaacaag
    18601 ggtattgtag caaaaattgt tcgcgatgag gatatgccat tcctagagga tggtactcct
    18661 gtggacatag tgcttaaccc ccttggcgtg ccatcacgta tgaacctcgg tcagatttac
    18721 gaaaccgtac ttggatgggc aggtcaaaag ctgggcatta agtttagtac acccattttt
    18781 gatggtgcta ctcttgaaga tattaccaag tacaccgata tggctgggct accccgtttt
    18841 ggtagaacat acctttacga tggcggaaca ggtgaaaggt tcgaccaacc tgctactgta
    18901 ggggttattt acatgcttaa gctcggtcac atggttgacg ataagatgca tgcccgttcc
    18961 attggacctt actcactcat tactcagcaa ccccttggtg gtaaggctca gtttggtggt
    19021 cagcgttttg gtgaaatgga ggtgtgggca ctggaagcat ttggcgcttc gcacatcctt
    19081 caggaaatcc ttaccattaa gtccgacgat gtagtaggaa gagctaaagc atacgagtcg
    19141 atagttaagg gagaaccaat gcctgaaccg ggcattcctg aatcgcttaa cgtattgctt
    19201 catgagctcc gtggtttagg gttgagtgta aatctggatt aatcaatatg ccgtggaaag
    19261 ggcaaaggcc ctttccggat tggcataaca aacttataac acgaataata tgtcgttcag
    19321 aagggagaat aaggttaaaa gcaacttttc aaaaatcacc attagcttag catcccccga
    19381 ggagattttg gagcgttcaa gcggtgaggt gcttaaacct gaaaccatta actatagaac
    19441 atacaaaccc gaacgcgacg gtttgttctg cgaacgcatt tttggtcctg tgaaggacta
    19501 cgagtgccac tgcggaaagt acaagcgtat caggtacaag gggatagttt gcgaccgatg
    19561 tggggtagag gttaccgaga agaaggtacg ccgtgaacgc atgggacata tcagccttgt
    19621 tgttcctgtt gctcatatct ggtatttccg ttcacaaccc aacaagattg gctacctgct
    19681 tggactacct gctaaaaaac tcgatgccat tatatactac gagcgttacg tggttgttaa
    19741 tccaggcatt aaggctgccg atggtattaa tcagtttgac tttttaactg aagaagaata
    19801 cctcgatatc ctcgatacac ttccaaagga aaaccagtac ctcgacgata gcgaccccaa
    19861 caagttcatt gcagctatgg gagctgaggc tctttacatg ctcctgcaac gcattaacct
    19921 cgatgaactt tcgtacgagt tgcgtcacaa ggcaagtact gagtcgtcgc agcagcgcaa
    19981 gagcgaagct cttaaaaggc tgcaggttgt tgaagcattc cgcgaatcgg ctcatataaa
    20041 caagcctgag tggatgatac taaaggtgat tccagtaatt ccacctgaac tacgtccttt
    20101 ggtacccctc gacggcggtc gttttgccac atcggactta aatgaccttt accgtagggt
    20161 aatcatcagg aataaccgcc taaaaaggct aatcgagata aaggcccctg aggtgatttt
    20221 acgtaacgaa aaacgtatgc ttcaggaagc cgtagattcg ctatttgaca actcgcgcaa
    20281 atcgaacgca gttaaaaccg aggctaaccg tcctcttaaa tcgttaagcg atagccttaa
    20341 gggtaagcag ggacgtttcc gccaaaacct cttgggtaaa cgtgtcgact attctgcacg
    20401 ttcggttatt gttgttggcc cagagttaaa gatgcacgag tgcggattgc caaaggatat
    20461 ggctgcagag ctttacaagc cttttgtaat ccgtaagctt attgagagag gtattgttaa
    20521 aaccgtaaaa tcggccaaaa agatagttga caggcgcgat ccagttgtat gggatatcct
    20581 tgagaacgtg atgaagggcc atcccgttct actgaaccgt gctccaaccc ttcacaggct
    20641 aggtattcag gctttccagc cccgtcttat tgagggtaag gccattcagc tccacccact
    20701 gtcgtgtact gcgtttaacg ccgactttga cggtgaccaa atggctgtac acttacccct
    20761 tggaaacgag gctattctgg aggcccagat actcatgtta ggttcccaca acattttgaa
    20821 ccctgcaaac ggtgctccta tcactgtacc ttcgcaggac atggtactgg gactatacta
    20881 cataacaaaa ccccgtaagg gtgccaaggg cgagggaaca attttctact cacccgagga
    20941 ggttatcatt gcttacaatg agaaagccgt tgacctacat gctattatca aggttaggtt
    21001 aaatacctta gatgaaaacg gtcaacctaa gcaacaaata attgaaacta cagtaggacg
    21061 cgtaatattt aaccaatacg ttcctgctga gatgggcttc attaatgagt tactgaccaa
    21121 gaaatcgctt cgcgatatca gtggtaaggt tcttaagaag accagcgtgt ccaaaaccgc
    21181 tcaattcctc gatgatatca aggacttggg atactacatg gcattcaagg gaggtctgtc
    21241 attcaacatc gacgatgtta tcattcccga tgagaaagcc gaattggttg aagaaggata
    21301 cgcacaggtt gacgaggtta tgtccaacta caacatgggt ctcataacca acaacgagcg
    21361 ttacaaccag attatcgaca tctggacaca taccaacgcc aagttgacct tgatgctaat
    21421 gaaaaagctc tccgaggaca agcaaggatt caactcaatt tacatgatgc tcgactccgg
    21481 tgctcgtggt tccaaggagc agattcgtca gctttgcggt atgcgtggtc ttatggctaa
    21541 accgcaaaaa tcaggtgcta ctaccaatga gattattgaa aacccaattc tttcgaactt
    21601 taaggagggt ctttcggtac ttgagtactt catctcaacc cacggtgccc gtaagggtct
    21661 tgccgatact gctcttaaaa cagccgacgc tggttacctt acccgtcgtt tggttgacgt
    21721 atcgcaggat gtaatcatta ccgagagtga ttgtggtaca cttcgtggac ttgtggctac
    21781 agccatcaag aagaacgagg aagtggttga aaccctttac gaaagaattc ttggtagaac
    21841 cacggtacac gacgtattcc atccgctaac cggcgaactt attattgcag ctggggaaga
    21901 gattactgag gaaattgctc aaattattga ggagtcgccc atcgagcagg ttgaaattcg
    21961 ttcagtgctt acctgtgaat cgcgcaaggg tgtttgcgcc aagtgttatg gccgcaacct
    22021 ggcaaccgga cgaatggttc agaaaggcga ggcagttggt gttattgcgg ctcagagtat
    22081 cggtgagcct ggtactcagc ttacactgcg aaccttccac gttgggggta ctgccggtag
    22141 catcatttct gactcaaaga ttgttgcccg tttcgatggt cgtgccgaga ttgaagagct
    22201 aaaaacagtt caacgtcagg atgaaagtgg caacaactac catatagtta ttgggcgttt
    22261 ggctgagatg cgaattgtgg attccaacac tggaatcacc ctttccacaa acaatgtgcc
    22321 ttacggttca aagctataca tcaacaatgg tgatacggta aagaaagggc aactcatctg
    22381 cgaatgggat ccatggaatg ccgttatcat ttccgaggtt tccggtaagg ttggattcga
    22441 cgctctcatc aaggaggtaa cctaccgcga ggaaagcgac gagcaaaccg gttaccgtga
    22501 gaaagtgatc attgaatcgc gcgataagag taagaaccct gctgtaaaga tcctttccga
    22561 taacgaggag gtgattaagc tttacaactt acctgttagc gctcacctaa ccgttgaaga
    22621 tggtgcaacc attcaggcag gtgatattct ggcaaagatt ccacgtgctg taggtaaagc
    22681 aggcgatatt accggtggtc tgcctcgagc agttgagctt tttgaggctc gtaacccatc
    22741 gaaccctgca attgtatcgg aaatcgacgg tgaagtatcg ttcggtaagg ttaagcgtgg
    22801 taatcgtgaa attaaggtta cctctaagac cggtgaggtt aagaactacc ttgtgcccct
    22861 gtccaagcaa atacttgttc aggaaaacga ttacgtaaag gctggtacac ctctttcgga
    22921 cggtgcaatt acccctaccg atattcttgc aatcaagggt cctactgaag tgcaggagta
    22981 cattgttaac gagattcagg aagtttaccg catgcagggt gtaaagatca acgataagca
    23041 ctttgaggtt atcgttcgcc agatgatgcg taaggttgaa atacttgatc cgggcgatac
    23101 acgcttcctt gaaggacaac ttgttgacaa gtgggagttc attgaggaga acgacaatat
    23161 cttcgaaaag aaggttgttg ttgatgctgg cgatgcagcc cacctacgtc caggtatgat
    23221 tattacagcc cgtcagcttc gcgatgagaa ctcaaagctg aaacgtaagg atatgaagct
    23281 ggttgaagct cgcgatgcag ttcctgcaac atccaatcag gtgcttcagg gtattacccg
    23341 cgctgcgctg caaaccaaca gctttatgtc tgcagcatcg ttccaggaaa ccacaaaggt
    23401 gcttaacgaa gcagccattt acggcaaggt tgacaccctt gagggattga aagagaacgt
    23461 tatttgcggt cacctcattc cagcaggtac tggtctcaga gagttcgata agcttgttgt
    23521 ttactcaaag gatgaacatc agcttattga gaaatcaaag agttcaacag ctactgttaa
    23581 gtaaatcaga tagattatag gaaggagccg gtgaatgccg gctctttttt ttgctcataa
    23641 tcgttaatta gcaaacaaat tcataactta gccgtgaaaa cattaaaacc gtccagctat
    23701 gaacgataaa gtaaatccaa tcaatattga acttagcgac gaggtagctc agggtatcta
    23761 cagcaatctt gccattatta cccattcatc ctcggagttt atcattgatt ttgtgcgtat
    23821 aatgcccgga atgcccaagg cacaagtaaa aacccgtgtt attctaacgc ccgaccatgc
    23881 taaaagactc ttgttagctt tacaggataa catggctaag tttgaggcta atatgggtac
    23941 aattaagtta cccgaaggaa gcgagggaat gcctccattc cccatgaatt ttggtggtaa
    24001 aggtggtgaa gcttaaaata cttttttgcc atgaacagag tttttaccat tacaatggct
    24061 tttgctcttg cctttgtttc gtgcaacaag agcgatagtt tagttgttct atcaccttca
    24121 ggcaaggtat ctattacatt tctgataaat ggtaaagggc aaccctcata cagcttgctt
    24181 tttgaccaaa aaacaatagt taaaccttca acgctaggtt ttgagtttaa ggatatgcca
    24241 cctttatcgg ataatattaa ggttgacagt ttcaaaacca gatcatttag tgagaaatgg
    24301 gaaatgccct ggggcgatca gcgatgggtt gataatacct acaatgagct taaggttttc
    24361 ctatcggagc gaaaatcgcc ttaccgtaat tttgctgtta cctttagggc ttttaacgat
    24421 ggtgtaggat ttcgctacga gttccctgag cagcccggaa tggatagcgt aattatatta
    24481 gccgaaaata ctaacttcaa tctaaccgat gaccacactt gctggtggca gcccggcgac
    24541 tgggatatct acgagcatct ataccggaca accccattat gtaagattga tgctgtaaat
    24601 agccagagta agaacccttt ggctcaaacc tatatccctg agaatgcggt aaacactcct
    24661 gttaccatga aaacctccga tggggtttac ctctgctttc acgaggccga tgtaaataac
    24721 tacgccggtg caaccctaag ggtcgatacg gttaatctat cgcttcaaac agcgcttgtt
    24781 ggaaacgatt caggcgtgaa ggttaaagct aaagtcccct ttaactcgcc ctggcgaacc
    24841 ataataatta cagataaacc aggcgatatt gtaacatcaa agctagtgct aaaccttaac
    24901 gagcccaata gaattggaga tgtatcgtgg tttaaaccca tgaagtatgt gggtatatgg
    24961 tgggaaatgc accttggcaa atcgtcgtgg gatatggcca gcggaaaaca tggagccaca
    25021 acggcaaacg caaagcgcta catcgatttt gctgcgcgta atggcataaa aggtttgctg
    25081 gttgagggtt ggaacaccgg ctgggagcat tggattggtt ttgaggaacg cgagggaatt
    25141 ttcgattttg ttactcccta tccagactat aatttaaaag aggttgtacg ctatgccaag
    25201 agtaagggtg taagcctaat catgcatcac gagacatcgg ctgcaccccg tacctatgaa
    25261 cggcaactcg attctgctta taggttgatg caaagccttg gcatacattc agtaaagact
    25321 ggatatgtag gaaaaattat tcccaaaggt gaataccacc aggggcaatg gatggttaac
    25381 cactatcgca aggtgattga aacggcagcc agataccaaa ttgctgttga tgcccatgag
    25441 ccctttaaag gtactggtga gtggcgaacc tatcccaatg ttattgctcg cgagaacttt
    25501 aggggacagg agtttaatgc ctggtcagtt gatggtggaa atcccccgga acaccttaca
    25561 attctaccct ttaccgcaca gcttgctggg ccaatggatt acactccggg catttttaac
    25621 atcaagctaa aaccctataa gcccgataat caggtaaatt caaccttagc ctaccagctt
    25681 gctctttacg tggtgatata cagccctatt caaatggttg ccgatttgcc tgaaaactac
    25741 gaaggtcacc ctgccttcca gtttatccgc gatgttgggg ttgactggga gcaaacggtg
    25801 gtattgggtg ctgccatagg cgattacatt gccattgccc gcgaggagcg ttattcagga
    25861 cgatggttta ttggagcaat taccgatgag aaccctcgag accttagcct aaaacttgat
    25921 tttctgaaac caaacgttaa atacaaagca actttgtacc ttgatggtcc tgatgctcat
    25981 tgggataaaa atcctacatc gtatagaata gagacccgcg aggtggatag caacacggtt
    26041 attgacctta aacttgcacc tggcggcggc gctgctattt cgcttttccc attttaataa
    26101 atagttaagg ctgccaattg gcagcctttt ttcctatctt ttcaggtatt tagcaaatac
    26161 gtataaacaa actgttggtg ttaaaacgcc aacaacgtag ccaacccagt gtgaaagttt
    26221 aaaaccctca ggagcaatag taatgtagtt gctaacaact gctgtcataa ataaagctgg
    26281 cagcagtgtt atgatgtagg gtttcttttc ctgtgctaaa taaacagtta ttgcccacag
    26341 cacaactgtg gcaagcacct ggttactcca gaacatgtat cgccaaataa tatcaaactt
    26401 tacctgagta agcgtaaatc caataataaa aagcggtaag ctcaccaaaa ccctattcga
    26461 taggcttttc tgcgagaact ttaaaaaatc ggcaacaatt agtcgagcgc tcctaaatgc
    26521 ggtgtcgcca gtagttattg gtgcggctac aactccaaga atggcaagta ttccgccaat
    26581 ggcgccaagt gtagtattgg aaatttccct tacaataata gtggcttcac cattgtaacc
    26641 tgcaatagct gagtttagcc cctgcgctcc cccataaaaa ctcattccta ttgcagccca
    26701 aatcagtgca ataatacctt cggttaccat tgctccatag aatacctttc gtccctcctt
    26761 ttcgtttttt aggcaacgcg ccatcatggg gctttgggtg gagtggaagc cgcttattgc
    26821 tccacaggag attgttacaa acagcattgg gaataatggg aacttatcgg cattggcttt
    26881 catgttgtgc atgttgttcc atatttcagg aatatcgtaa aggtagccac cagtaaatat
    26941 tgcaatggta attccaacag ccattatgat gagggctaga ccaaaaatgg ggtaaaaacg
    27001 accaattatc ttatcgatag gaagcagcgt tgccagaatg tagtatgtga gaataataaa
    27061 cacccaaaca tttttggtaa gatagtcagg ggttaacgat gccataaggg ctgctgggcc
    27121 tgaaatgaat gctgcaccca caagaatcat tagtattagc gtaaatactc gcatgaattg
    27181 ttttacacta atgccgaggt acttcccagt gatttcagga atactaagcc cgcccatgcg
    27241 tatggaaatc attcccgaga aataatcatg aacggctccg gccaggatat tacctagtac
    27301 tatccataaa aatgctactg gtccgtaggc tgctccaagt acggcaccta caatggggcc
    27361 aagaccagca atattaagga attgaataag gaatgctttc caccagggca tgggaatata
    27421 gtcaacgcca tcctgcatgg tgtaggcggg ggtttggctt tggctgtttg ccccaaatac
    27481 ccgctcaaca aacctgccgt ataggaaata acctaatagt aagcccaaaa ctgctagaat
    27541 gaatgagtac atagatttga ttttttttta gccaaattta tttatttcgg ttttattttt
    27601 tggggcagca ataaaaaaaa gcagctattc acggctgctt tttctcattt atcatggttt
    27661 agtttaaacc cctattcttc agcagtggtt caatgctagg ctctttacca cggaaatcct
    27721 tatacagctc catggcatcc tttgaaccac ccttttcaag aacgcaagtg cggaacttgc
    27781 cagcaatttc tttgttgaaa atatcgccgc tttcaacaaa tgcttcaaaa gcatcgctat
    27841 cgagtacctc ggcccaaatg tatgagtagt aaccgcttga gtaacccata acatcgctaa
    27901 atatatgtgc aaagtatgtg ctgcggtaac ggggccaaat ctctggtatc agcccgatct
    27961 tgtccataga ggctttttca aaggctacta catcttcaat ctttgcctta tcagccgtta
    28021 gtgtgtggta atccatgtcg aggatcgatg ctgctaaata ctcggttgtg gcaaaaccct
    28081 ggttaaaatg tccgctagct tcaatcttat tgattaactc atcgggcatg ggctctccgg
    28141 tttggtagtg gcgtgcatac tctttcaggt attgtttatg gccagcccaa tgttccataa
    28201 tctggctagg taactcaaca aaatcgcggg gcacgttgcg taggccaggg tactgcacct
    28261 tactaaaaag cgcatgtaat gcatgcccaa actcatggta gaaggtttca gcttcgtcat
    28321 agctgaggag tgctggtgag tttcctgttg gtttgctaaa gttacatact atcgatacta
    28381 ccggtgaaat aaacttacca tcgggcatta tacgttggct gcggtactcg gtacaccaag
    28441 ctccaaccga tttgctttca cgggggaaga agtcgaggta tagtatgcca acatgcttac
    28501 catcggcttc ctgaacctcg taaacctcgc attcagggtg atatactggt aggtcggtac
    28561 gctttacata cttcaagccg taaagttttt cagtgaggtt gaaaatccct gaacgaacag
    28621 catcgagcga taggtatggg cggagcattt cttcgtccaa atcatatttt gccttacgca
    28681 ctttttcggc atagtaccac cagtcagcag ggccaatttt atattttgca ccttcggctt
    28741 tggctagttt ctgcatatcg tcacgctcag ctttagctct tttaagtgct ggttcccaga
    28801 gcttgttcaa gaggttgtaa acattttctg gtactttggc catgttaaca tctaaaacgt
    28861 agtgagcatg ggtaggataa cccaaaagtt cagctttttt ctggcgtaaa tctactattt
    28921 gctttattat attcttgtta tcgtgagcat tgttgttatt accacgcatg taatagccat
    28981 agtacaactt atcccttaac tctttgttct gtgcataggt taggaaaggt atcatgctgg
    29041 gtttgtaaat ggtataaacc catttgcctt ccattcccct cgatttggct tcctctgcac
    29101 ccatcgcttt tactgattcg ggtaatccag ccaaatcctc ctccttatca atcacaataa
    29161 agtaatcatt gttttctgct aaaaggttct cattgaactg aagggcaagc agtgatagct
    29221 tctggttaat atcacggagt tcggcttgct tagctgaatc aaggtttgca ccacctcgaa
    29281 caaacgattt gtaagttttt tcaatcagca acttctgttc tggggtgtaa ttgttttggg
    29341 ccatgttctc gtaaacgact ttaacccttt caaatagctt gggattcatg ctgatatcat
    29401 cccgatgttt cgaaagcatg ggcgatacct ttttaagaac ctcctgtagc tcatcgctgg
    29461 tattggcaga gttgatattt tccatggtta aaaccactcg ctcaagcagc tctccgctgt
    29521 aatcaagcgg tatgatggta ttttcaaagg taggagcttc agcgttagct gttattgcat
    29581 caatttcgct caactgctgc ttaatgccct cagcaatagc aggctcatag tgttctactt
    29641 taattttatc gaatggcgga acaccaaagg gggttttgta ctcgccaagg aaaggattct
    29701 ctggaccttt ctttcctgta catgccatca ttgttcccat taaaccaatg gttagaatta
    29761 gtaaaccttt acttttcatc gtctaaaagt ttaagtattg atagttagat ttttaggttt
    29821 gattttaatt tcccgaaagt tagcaatatt gattatattt ttatcaaccc gggtaatgtt
    29881 tcgatttaat atcacccata aagtttactt tgtctctaat tttttaattt ttgggttgat
    29941 gatagttgat actattttta tgaatcggtt tagaatatta ttttactttt gcactctgtg
    30001 aatagatgtt taacaggtaa aaatgatgta ttatgggagc atttcatgcc tacgacatta
    30061 gaggcatata caacaaggat ttcaataaag atgatgccta taaggttgga ttctttttgg
    30121 ttgacctgct aaaaacgaac cgtgtactgg ttggtcgcga tgcacgcgaa tcgtccgatg
    30181 aaatattcga gtacctcacc cgtggtatca ccgatgccgg agccgacgta tacgatcttg
    30241 gcttggcaac aacacctatg gtttactatg ccactgcaaa gggtaatttt aatgcatcgg
    30301 tgcagattac tgcctcgcac aatccccgcg agtataatgg aatgaaggtt tcccgggaga
    30361 atgctgaacc tgttggttac gataccggcc ttaaggtaat tgaggaaaag attaaaaacg
    30421 gtgagcctat tgttcctgct tcaagcaaag ggaaggttat tgagcataat attagagcag
    30481 agtatatcca attcctaaac ggctttaaac gcgattattc caacctcaaa atcgggattg
    30541 attgctccaa tggaatggct gcacttatca ttaaggatat tcttggcgca agcccaattt
    30601 acctatacga tgagctcgat ggcgctttcc ctaatcatga ggccaacccc ctaatcccag
    30661 agaatgtggt tgatctgcag aatttggtta aggctaatgg ttgcgatgtg ggtatcattt
    30721 tcgacggcga tgccgaccgc gttatgtttg ttgacgagaa tggtcgcttt atttcgcccg
    30781 acctcatgat aggcgtatta ggtcactact ttctggagga gaaaggacta aagggtaatg
    30841 ttttacagga tatacgcacc tccaaggctg tgggcgagta tttatcacca atgggggcaa
    30901 caatgcatac ctggagggta ggacgtgcgt ttgccgctaa aaagctacgc gagattgatg
    30961 gaatttatgg tggcgagctt gcaggtcact actatttccg cgatttcttc tattccgatt
    31021 ccggcatgct ggcttgcata ctcatactaa acgttgtgag caagatgaaa gctatgggga
    31081 tatcgctatc gcagctcata tcacgaatcg agacctatgc aaattcaggc gaaatcaatt
    31141 ttaagcttga gcgcaagcgc gaagccatgg atgccgttcg cgagcatttt acctctgtgg
    31201 agcaacctac tgcttcgttc gattttgatg gttaccgtgt ggagttcccc gattggtggt
    31261 ttaacattcg cccttcaaac actgaaccct atctacggtt tatagctgaa gctaaaaccc
    31321 acaaaatgct tgaggaaaag gttgctaagg tgcgtgaaat tattgctaaa ttcagctaat
    31381 acatatatat gaatatgaat cggctggaaa taaaaggtaa acatggaact tacctggtaa
    31441 atctggccga ttctacatct gttttaaagc caagcggtga aatttgctct acctatattg
    31501 gctacgaaaa acaatcgggt aggagagttg ttgtaaaacg gtatcaccct tgggttaact
    31561 ccgcttccgg ttacttttgg cgcatcgagc gcgaggctca agcaacctct gcatgctcgg
    31621 gcttatcctc cgagttgata ctattggagg gcatttacta cttggttacc gattacattg
    31681 aaggtgttag ttttaaggaa cttacccgct ggcgatacca tcgtaagcta aataactacc
    31741 atctcatata tcttgcttcc aaagcattag atgcattgca aaagatccat gataccggtt
    31801 ttattcattg cgacattaaa ccatcgaata tcattttaac tgatccaaaa aatattgcaa
    31861 acaccgatgt tagaataatc gattatggtt tagcacgcaa acctgctgag ccattacata
    31921 gcaatgaaaa agctctcgct tttggtttaa tatattccgc tcctgagcag gtgttcaatt
    31981 tttgggattt aatcgacatt caaaccgata tttactcaat gggagttaca ctttgggcgc
    32041 tattttcgcg tagcgagcca tgggtggcga gtaaccctct taaaactatt catgttcagt
    32101 taacccaacc cttaccaggg aaccgaaggg ttgctgatga tttaatgagc atattgttaa
    32161 aatcaacatc aaaggctcaa ttgcccaagc caccgcacta ttacacccga acccagttaa
    32221 gagccttatt gcaacaagcc aaaagtatta ggtatgctag tgctgtagag atgttaaagc
    32281 atattgaaca actgggaaag aggtagccaa aatgttaaaa aagttttgct gctgtattgt
    32341 aatttaaaaa attcagtatc tttgcaccgc aaaacaatgg cggggtgtgg cgcagttggc
    32401 tagcgcactt gcatggggtg caagtggtcg tccgttcgag tcggatcacc ccgaccaagg
    32461 tgaataagga ggcagtcatg cctccttttt tcatttcctg cagttg
//
LOCUS       DAOSAL010000022        32325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_1010_length_32325_cov_47.476480, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000022 DAOSAL010000000
VERSION     DAOSAL010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 32325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32325
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1010_length_32325_cov_47.476480"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1370
                     /locus_tag="PK049_04820"
     CDS             <1..1370
                     /locus_tag="PK049_04820"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOK60911.1"
                     /translation="YHVHCPWDNSPIAKVHLAGEKELEQAILLAQQAERPMAELAAFE
                     KHEILMRIAQGIKENRNSFAKTLALEACKPIKLAYGEIDRAIQTFIIAAEEAKRPPAE
                     LIRIDWTPQGKGKEGLVKCFPVGLIAGIAPFNFPLNLSVHKIAPAIAAGCPIILKPAR
                     STPLSALKLAEIIDSTPLPKGALSILPTNREVGSKLVTDSRFALLSFTGSPQVGWNMK
                     NQAGHKRVVLELGGNAGVVVTPSANLEQAVSKCLSGAFAYAGQVCIHTQRIFVHQAVF
                     TAFTEKFLQGTANLKNGHPLDTDTDISSMIDEENAIRVEEWVNDATNAGAKVLLGGKR
                     KGSFFQPTVLTNTTNSMKVCALEVFGPVVTLEPYTDFHEAVNLINQSEFGLQAGVFTN
                     LIDEMNFAFNELRVGGVIINDVPTFRVDHMPYGGIKNSGFGREGVKYAMLEMLEPRLL
                     VKNIL"
     gene            1446..2096
                     /locus_tag="PK049_04825"
     CDS             1446..2096
                     /locus_tag="PK049_04825"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOK60912.1"
                     /translation="MNETTMLLTLLIKGMIIGLAASIPLGPIGVICVQRTINKGKISG
                     FLSGLGAATADTLFAAIAGFSLSFIISFLEERQTIIELVGGVIVVFLGLKIFSTNPIS
                     QLKRNKRKKSNLLEDFMSVLLLTGSNPLAVFFFIALFATAGVISHDQSIWVNLIALAG
                     VFLGGALWWYILTSTVNYFRRHFRLKQLWWINKISGAVIFTLGGLAILNVIHKVFF"
     gene            2246..4036
                     /locus_tag="PK049_04830"
     CDS             2246..4036
                     /locus_tag="PK049_04830"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOK60913.1"
                     /translation="MKIANKLIEVLSFGVDTNKSKDFADKITQLNLIAIPSLLIFIIL
                     AITSLKASLPVAIINFSTFIVALVLLFMLFRSSRFSSIGLGISYIFAIGILVNILLGS
                     NNLLLLAVTMLLPLMFFSSTPFNKASVTLVAFAVLLPIVLFILPVIGLPAENIFYVNR
                     YFISIGFIVYLTSVSLLWFIRTKQLQELEIKSIELKNDIKKRTENLAKLSHDIRTPLN
                     NIVIISNILSNQIQEEKLKDMIDTIQASSNNLMSTLNSMIDLSISEMPKDSDVLVPFD
                     LQNTLNNTFRLFSTQSGTVSFSFKYDERLPKNLLGDPVKLKQIFLNIVESIIKNKSSQ
                     KVILDIKINKMQEAGDGVDIYTEIKTNTPLLLHHADGVSSTTLTDGSTALSNQVFIEM
                     LDLKIAAKLIRENGGKLNVKLTSQDSQINFNIRLLKVPTALKEEIQQAEGKEKGINEI
                     VPETPEVSKVEKLPTTDVRLENANVLLVEDNLINQKIVLLSLKKLVKNIDVAVNGKEA
                     LDKFGTSKYDIILMDVQMPIMNGYTAAKKIREIEASTNSHTPIIAITANALMGDREEC
                     LAAGMDDYISKPFQIEVLIQKMRNLLEANR"
     gene            complement(4033..6336)
                     /locus_tag="PK049_04835"
     CDS             complement(4033..6336)
                     /locus_tag="PK049_04835"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOK60914.1"
                     /translation="MCRSKYIVVFILFLLAPGLNAQKVSLVLSGGGAKGLAHIGVIKA
                     LEENGIPIDNVSGTSMGAIIGGLYAMGYSPDEMVKLFKTRDFYNWSKGIIDESLKYNI
                     NDFSSSDAENLSVGLVLTSKGIKPKVASSFIPTVGMDIAFDELFSQGTALSGGDFNKL
                     FVPFRCNASDVVGKNIVYFRNGDLGRSIRTSMTFPLYYKPIYVDSLLLFDGGIYNNFI
                     WPEALKEFSPDFIIGSKVANNSKIPSDDDPFEQLEAMIVGMTNYDIPDSIGMVIDTRL
                     PNVNLWDFDRVDEIVGLGYQSAINLMDSIKAKVSRRVSLDSLAHARKQFREKLPPLYI
                     SQISVLGLNPKQQKYIDRVLMSKFSIVTYDRFKVNYYRLMSDKVFARIQPQFVYDRNS
                     GLFDVKMQAILNRHPSLGLGLSLSSDNGNEGFFSITHNWLTRTSNTLYGNIYFGKFHS
                     SARLTAMKTFPSRIPVSLVSTLVANRYDYHSGNPVPFFEDVKPAYAIQNETLVTIALR
                     LSHTSALNTSMFVTSGEKMDEYYQTENYYSFDIPDKTRFRFFKGGIRLEKNTLNHKQY
                     ANRGRHQLVSISGYYGHEIHKPGTTAMQLSEFDNYQSFITAYLHNESYHRLIGKSFWL
                     GFYLDGYWSSQRFFDNYWATILSMNQFSPTPHSRIMFLKELRSSKYVAIGLSPVVDFG
                     SNVSFRIDAFVYQPYKAILANSDGLAYYGDAFKSREYLMSASVIYSSPVGPVTITVSH
                     YPRNKGKQFFLNLSFGYSLFNPRVFDN"
     gene            6697..8076
                     /gene="glmM"
                     /locus_tag="PK049_04840"
     CDS             6697..8076
                     /gene="glmM"
                     /locus_tag="PK049_04840"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOK60915.1"
                     /translation="MTLIKSISGIRGTIGGRVGENLTPVDIVRFTAAYAEWLKQRNNG
                     KRYGVAVGRDARISGEMVNNLVIGTLLGCGVDVLNLGLATTPTVEMAVIHEKTDGGII
                     LTASHNPRQWNALKLLNEKGEFLSDTDGRKVLEIADSESFSFADIDNLGITDEEYSFI
                     DEHIKHIINLKYVDTDAIGKANFRVAIDCVNSVGGIALPRLLKALGVKDIIELYTDPT
                     GDFPHNPEPLPEHLTDISKVIKERRADVGFVVDPDVDRLAIINEDGTMFGEEYTLVAC
                     ADYVLSQNPGNTVSNLSSTAALRDITLKYGKEYFASAVGEVNVVTKMKEVNAIIGGEG
                     NGGVILPELHYGRDALVGIALFLSHLAKSRMKCTELRRQYPSYFISKNKIELKQGLNP
                     DAVLERLKEKFSTEKINTIDGLRIDFAEGWVHMRKSNTEPIIRIYAEAQTAAKANELA
                     QQIIKEATK"
     gene            complement(8132..8569)
                     /locus_tag="PK049_04845"
     CDS             complement(8132..8569)
                     /locus_tag="PK049_04845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOK60916.1"
                     /translation="MSVNKVILVGNVGKDPEVRHLEGGATVARFSLATNETYTDKSGK
                     KVTQTEWHNIVVWRGLAEIAEKYVKSGKLLYVEGRIRNSSYEDKEGNKRYTTEILCDN
                     FRFLGPSTGQGEKPNNYNESHGTDVGAAESDAEIPQPDDDLPF"
     gene            complement(8635..9711)
                     /gene="mutY"
                     /locus_tag="PK049_04850"
     CDS             complement(8635..9711)
                     /gene="mutY"
                     /locus_tag="PK049_04850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOK60917.1"
                     /translation="MDFSSILLKWYMANARDLPWRNTSNPYYIWVSEVILQQTRVVQG
                     ISYYYRFIERFPTIQSLADAHIDDVMKVWQGLGYYTRARNLKAGAIQVVEKYNGSLPT
                     SYSELLRIKGLGAYSAAAVASFAFDEAVPAVDGNVYRILSRIFGIFTPIDSTSGKKEF
                     FALANELISRTEPGRFNQAMIDFGALQCTPKSPRCPECPFADYCYAFQNNLIAALPVK
                     GKKKPPTDRFFYYFMVKHKGYTYIKRREQKDIWHSLYEFPMHETSAALKPDELIEMLN
                     SLSFLSGSKVKVLNISQPVRHILSHRTIWATFLILEVDKPNYVLADEYVRVPIDNVNN
                     YSLPRVIDAYMAAEPALKYFSTKK"
     gene            9848..10135
                     /locus_tag="PK049_04855"
     CDS             9848..10135
                     /locus_tag="PK049_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOK60918.1"
                     /translation="MTKADIVNEISKSTGIEKVAVQKTVEAFMEAVKDSLVKEKNVYL
                     RGFGSFIVKKRAKKTARNISKNTTIIIPEHFIPAFKPSKSFVNKVKNHVKK"
     gene            10359..11909
                     /locus_tag="PK049_04860"
     CDS             10359..11909
                     /locus_tag="PK049_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005681476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOK60919.1"
                     /translation="MNTELIIDVRETEVAIALLENKQLVELNKERSNVQFSVGDIYLG
                     KVKKIMPGLNAAFVDVGYEKDAFLHYLDLGPQFKTLQKYVQDHYSRKNKTIPISKYGM
                     LPDIDKSGKITDVLSTGQPVMVQIAKEPISTKGPRLTSEISIAGRNIVLVPFNDKVSI
                     SQKITAPEERKRLKRLMESIIPKNFGVIVRTAAEGKKVAVLDAELRSLIKRWEITVEK
                     LKNPGYPVLLTSEIKRTSAILRDMLNGSFNSIHVNDENVYEEIREYIESIAPEKEKIV
                     KLYNGNVPIFDQFGVEKQIKSLFGRTVSIKSGAYLIVEHTEALHVIDVNSGNRSKSSN
                     DQETNALEVNLAATDEIARQLRLRDMGGIIVVDFIDMHSNENKQMVYERMKELMSRDR
                     AKHNILPLTKFGLMQITRQRVRPEMHIDTSEKCPACNGSGKVTATILLDEQIENQLSL
                     ICASKKYRSVTIKLHPYIAAYIKKGFLSKRIKWALRYKCAINLLPMSSYHFLEYHFFD
                     KHDNEIEL"
     gene            12263..12682
                     /locus_tag="PK049_04865"
     CDS             12263..12682
                     /locus_tag="PK049_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60920.1"
                     /translation="MKKALMVFIPLMAMFKMGDAQNLSFGAELSSNFHLESILPEVGL
                     KLNVPLSKHSQFETGLFYQIRKNDGAFSINNDLYIFDVNEWYLVVPLMYKFSSQIINL
                     SFGINYNYYVGWGVASVSDENVQINNYNREDTYLFGV"
     gene            12979..13518
                     /locus_tag="PK049_04870"
     CDS             12979..13518
                     /locus_tag="PK049_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60921.1"
                     /translation="MKRISYILMLSILAFGMAACEDDESGDYDNSVTISGSVTIDGKT
                     YSSPTFNMGTSMEVEGYTYLTNNPTAQIYYTAIYPKTEMVDAGNGLMAHYYFVLEDDQ
                     PGTASFTGMIMFHKNDSFVFGLWSQDVDVTIDKIDPVGGFIEATYNGTFTDEGNTPKT
                     YTVTGKIKAKRVSQPIIDK"
     gene            complement(13545..15800)
                     /locus_tag="PK049_04875"
     CDS             complement(13545..15800)
                     /locus_tag="PK049_04875"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HOK60922.1"
                     /translation="MRKLVMLTLALTALLSCSRNSDVNIIPKPVSIKQVDGDFTINSK
                     TLVRADSADSIAFNNAIYLVELLNQKGVGCAFNAEVGVDSTNSIVFTRTGCPDSLGAE
                     GYMLSIGPKHIVIKANESAGFFYAIQTLRQLMPANFEKNDFRQKSVTVPCLSIIDKPR
                     FPWRGLNLDCCRHFMTKDFVKRYIDLLAYHKMNVLHWHLTEDQGWRIEIKKYPRLTEI
                     GAWRTNADGTVYGGFYTQDDIREVVSYAAKRHVMVVPEIEMPGHSVAALAAYPNLSCT
                     GEPLKVETNWGVFKDIYCAGNDSTFIFLQDVLTEVVELFPSPYIHIGGDEAPKYRWEN
                     CPKCKARLKSEGLSNYEELQRYFISQIAKFLEGKGKTIIGWDEILEGGRPHNATVQAW
                     RGIDRATKALSANAPVIVSPTSHCYFDYPVEVTNLQKVYSFDPVPAQVDSAKIQLVLG
                     GECNMWTEYAPQETVDSKVFPRILAIAEVLWTYPRERNYNEFLNRVRNHYHRLEALGV
                     TYGFEQSAVEFKSSIGDDKKSIFVELIKGQDNLTIRYTTNGDEPTVNSTRYCKPIELT
                     SSTTLRAAAFNNGNRVGDVFERSFVVSKSLGKPIELGFVPAEKYLGGSKQALVDGRLG
                     TPKYNDGIWQAVQGHDMVATIDLGANQPIKNFSVGFLQNILSWIFLPNRVDVFVSADS
                     ISFEQIAQMDCPENIKRQPVLVYRFNAFVVNNIMARYVRFVAHNPGPCPEWHPASGSP
                     TWLFADEIVVE"
     gene            complement(15813..18761)
                     /locus_tag="PK049_04880"
     CDS             complement(15813..18761)
                     /locus_tag="PK049_04880"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HOK60923.1"
                     /translation="MKPLALVTVLMQAVSLWAQKGPADYVNPFIGTDFHGHTYPGATV
                     PFGMVQLSPDTRLTGWDGCSGYHYSDRVIYGFSHTHLSGTGASDYGDILFAPVTGKSP
                     IFENSKYSSPFNKKNEQAKAGYYSVFLDKYKVKTELTTTARVGFHRYTYTKPNEPAMV
                     IDLSHRDKVLEAWFKVTGKNEIRGYRRSSHWAKDQKIYFVARFSEPFALGPKFTDDSG
                     EMNTLNNEDGTVEVHGTKLKAWVQFPKLKNKVLLVKVGISFVSEEGALKNLEAEVPGW
                     DFDGVVEAARNKWNEELSKIQVDGGTPEELTTFYTAMYHAMVVPNIFSDVNGLYRTMD
                     GNVRYADGFTPYTVFSIWDTYRAWHPLMTIINTKRTSDYINTFLSHFRYGGRLPVWEL
                     AANETDCMIGYHSVSVITDAWIKGIRGFDAEYALQAMKHSANLNHFGLQYYKRYGFIP
                     GDMEHESVSKTLEYAYDDWCIAQMAKGLGKYDDYNTFIKRAQSYKNLYDPDTRLMRPR
                     LNGGFKQKFNPAEVDQHFTEANSWQYSFYVPQDVTGLIKLHGGMDKFEAKLDELFNAP
                     SQLAGRNQVDITGLIGQYAHGNEPSHHMAYLYNYVGKPWKTQQLVKRIMKEMYSHKPD
                     GICGNEDCGQMSAWYIMSAMGFYPLCPGSTQYAIGSPLFPKITINLENGKKFTLIAEN
                     LNNQNIYIQKATLNGNPITRSWIDQAEIMAGGELVLTMASTPSDTWGTQPADLPSTSI
                     NDNLITIAPFLVAASDVFTDTLTVSIVSVQPDAQIFYAVEPVDKPLPQTNWIQGNKIN
                     LTESKKIRTFAILPNGQTSAILEGVFRKVNSIANVQLKSKYSSLYTAGGDLGLVDGVR
                     GSKNFRIGGWQGYQGQDFEAIVDLGEVKPVKQLSAGFLQDASPWIMMPREVSFSVSTD
                     GQNFVVVGTVKSDIPENQMGPTIKDFTVNVDTKARFVKVFAKAFGPLPSWHESAGEPS
                     WLFIDEISIK"
     gene            complement(18792..21101)
                     /locus_tag="PK049_04885"
     CDS             complement(18792..21101)
                     /locus_tag="PK049_04885"
                     /inference="COORDINATES: protein motif:HMM:NF012906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HOK60924.1"
                     /translation="MKKAISVLVLWVMAFAALSQQEFPLNGQWQFRRANSSDGWLPAT
                     VPGAVHTHLHSNGMIGNPYLGCNNMHLAWIDSADWEYRKVFALPKTFSSIRDVELVFE
                     GLDTYADVYLNGVKVLTADNMFRRWVVPCSKFLRKGKNELRVVFRSAVRMVDSIAGRG
                     SYRLPGGEWAYVRKAAYHFGWDWGPRFVTCGIWKPVYLRITPKVRVSNLSISTISVSN
                     QKALMAIDFDIDGDTKAQLRLTVTDVYTDKIYLDTAMRLKRHEVGARFYIDNPKLWWP
                     NGMGEQHLNRFRVQFFEDRKLVYDKVHDVGIRTVRLISEPDSSGQSFYFLVNGLPMFA
                     KGANLIPPDFFVHSIADSAWVQQVDFAAQSNFNMLRVWGGGVYAPDAFMEECTRKGIL
                     VWHDFMFACSMYPYSSAFLTNVKEEAVEQVKRLRKHTSLALWCGNNEVDEGWHNWGWQ
                     KQLSDYTGAADSVWNGYVQLFLHLLPEVVMRYDSTRSYWPSSPMFGWGRAESTTHGDS
                     HYWGVWWGREPFERYAQRIPRFMSEYGFQGAPSEETLRMFNDRNNLPDSAQMLCHQKH
                     PVGYQTINEYMEREGLKPNDLLEWIDYSQLVQAQGYAHAIEAHRLASPYCMGTLYWQL
                     NDCWPVVSWSGIDYAERWKRVQYYVQNLYKPVIAGAHLEDTLVVVKAVSDSIKPIQAS
                     ISAKVFDINGNELNQFNRNLTLQPNVAVEVGRIPFANELVVRNDIYLKLTVSIDNETR
                     FESVYYFTRSPHKDILGMGMVQRIREIKR"
     gene            complement(21384..>21688)
                     /locus_tag="PK049_04890"
     CDS             complement(21384..>21688)
                     /locus_tag="PK049_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOK60925.1"
                     /translation="SNAWKYFAPAMLSQLNGTISRTDGARVDRLEVKAFDIEDEAQRA
                     RFLSGQQQRVSVPFSNRTVEYDPMKRIGVGLSRLGTSHAVAVGAYVYALAMIDKKR"
     assembly_gap    21689..21788
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<21789..22648)
                     /locus_tag="PK049_04895"
     CDS             complement(<21789..22648)
                     /locus_tag="PK049_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOK60926.1"
                     /translation="MDYRNDNRVVLTLDAGGTNFVFSAIRGMERIGEPITLPSNGDNL
                     DKCLSGIIDGFSSLMNGLTEKPVAISFAFPGPADYPNGIIGDLGNLPAFRGGIALGPM
                     LEDRFHLPVFINNDGDLFAYGEAIAGFLPYVNQLLAESGSNKRYRHLLGITLGTGFGA
                     GLVVDGNLFIGDNSAGAEIWLVRNAIDPDCFAEEGASIRAVQRVYHSYSKHSHGKPIT
                     PKDIYEVLEGKQEGDLVAARLAFQTLGVVVGDAIANAVTLTDSLVVIGGGLSNAWKYF
                     APAMLSQLNGT"
     gene            complement(22648..23793)
                     /locus_tag="PK049_04900"
     CDS             complement(22648..23793)
                     /locus_tag="PK049_04900"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOK60927.1"
                     /translation="MNRKTFAILLAFLCMGFGDAVGPFVGLAKEQFALSNFEAQLIAF
                     MGFIMFGLLSVPMGLVQDRTGKKFVLMLGLSIALVGLVLPLFGLSSYALFLLTILLLG
                     AGAAILQVAGNPIMRDVSEPGKYSRNLSFGQFIKAIGSLSGSLIPFAAAKWMGMDWKV
                     LFPIYVASLLITLIIIAFTRIQEVKADKEPATLASCFSLLSNRFVLLMVLGIFVYVGI
                     EICLSSGVPILLSSHYGIDLKTWGLLGNAFFFIAILTGRFLGSVILSWVKPHSFFRMT
                     VALSVLGLLLIFVDNQVVTLAGVFITGLGFANIFPLIFSITVDSMPERANEISGLMVT
                     AIAGGAFIPPLMGLLADATSVRLGFIVPLAGIAFIALLTMVKPKTQS"
     gene            complement(23818..24843)
                     /locus_tag="PK049_04905"
     CDS             complement(23818..24843)
                     /locus_tag="PK049_04905"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOK60928.1"
                     /translation="MANRVTLYHIAQHVGVSKTLVSLVLNGKAQQYGISKQTCDKVFA
                     AVKELNYRPNRTARSLRTGKTETVGLIVSDISNAFYAQMARMFEDLASAQGYSVIICS
                     TDEEVEKEQRLITMLRNRQVDGLVVSTSQREGEWLKAVYDSGTPLVLIDRHLGSGTLP
                     AVVADNRSGGAMLAHHLVEQGYKKPLVLGLSTSHISSVAERIDGFLCVFKEHNLPCLV
                     REVGFNAIQADVDELLLNVKSLPFKPDCLFAVNNNIATAVLHSLAASNVKIPSDMAMV
                     CFDDLPYFSFISPTITAIEQPIAQMCSRAFEVLSQLMDGESYLSHEVIRLPVKLNERQ
                     SSQNKSK"
     gene            25771..25911
                     /locus_tag="PK049_04910"
     CDS             25771..25911
                     /locus_tag="PK049_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60929.1"
                     /translation="MGTPRVELPRDTIRHAYGVTHLVSLTKPNATFHPRPIAGSGLYA
                     TA"
     gene            26205..27005
                     /locus_tag="PK049_04915"
     CDS             26205..27005
                     /locus_tag="PK049_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60930.1"
                     /translation="MRKCFLLFWLLAIIAGNSNLRAQVLLPVPDSLRVINFRVTDYYS
                     HEPIGLAHVMNITQKKGCISDLLGYFSIPFKLGDSLSITAIGYHTKHVLNWGQFNKDT
                     LFYELTLTPKVYQIEEVKISRFSTYDRFLREFANLKLTKTRVTEQQEVIQLYFLKITK
                     GLNLRNLPQPTMGLSFGKDWYQKQNEKVAEAIEKDRKKRIADRKFNAGIVSSLTGLTG
                     NELQEFMEYLKFDENYILTSTDYEIRERILDRFKEFETIKKGKNNTKK"
     gene            27011..27976
                     /locus_tag="PK049_04920"
     CDS             27011..27976
                     /locus_tag="PK049_04920"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOK60931.1"
                     /translation="MAMQDVTTPSHLDIENAFNLIAKHINSTPVLTSRSVNTILGCNA
                     YLKCENFQRVGAFKFRGATHAILRLGSDEAHNGVITHSSGNHAAALALAARQRGIPAY
                     IVMPENAPEIKRKAVAGYGGIITFCPPTQADREQTMLRIKSQTEATFIHPYDNFNVVC
                     GQGTAALELMQQEPELDIVIAPVGGGGLLSGTATFIKGRFPHVKVYGAEPKNADDAYR
                     SLRDHTIYPSVKPQTIADGLLTSLSPLTYTILSQHCDGILTVSEQSIIEAMRMVWERM
                     KIIIEPSSAVPVAAIMENAQLFRYKRVGVIISGGNVDLSHLPWQG"
     gene            complement(27982..29064)
                     /locus_tag="PK049_04925"
     CDS             complement(27982..29064)
                     /locus_tag="PK049_04925"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOK60932.1"
                     /translation="MLIVRLIHRILVGVNVLFALLLVLTYVSVYINPEDFWFVALYGL
                     LYPFFLIVNLLFLFYWIFRWQKLVFISLAAILLGVSHFNGFIRFPYGSAAKGSNPDLR
                     VMTYNVNLFRLYSWSKLKPSYSDILTHIKDNDFDVVCLQEFYLREGKLSHLDVEQQSG
                     MKVYSSFILRRPSSAYGLAILTNQPVVSVGEISFPNSFNACMYADIVKGTDTIRVYNL
                     HLQSTRLKERNFNFLLRNEFKLDSKNYDELKDLLSRLGVAFTKRARQVNMVVEHMRTC
                     RYPIIICGDFNDSPVSYTYHTLTASLHDTFKDAGSGVVVTFSGLWPSYRIDYVLRSRQ
                     FRTVTYQSPRLRYSDHFPVVVELQLQ"
     gene            complement(29064..29939)
                     /locus_tag="PK049_04930"
     CDS             complement(29064..29939)
                     /locus_tag="PK049_04930"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOK60933.1"
                     /translation="MTIWDEIKSSFRLGSNLTRLIYINLGVFLLFGLLRVFLFLSASS
                     SGWITGFMAVPANLQSLMVRPWTIVTYMFYHEDFLHILFNLLWLYWFGQLFLQFFAQR
                     QLTWVYLLGGISGALLYILAFNLLPPFKGYAEVSLALGASASVLAVVIAVATLQPNFT
                     VYLLFLGAVRLKYLALATVVIDIISIPVSNAGGHIAHLGGALFGFLYVAALRSGTDLA
                     KPFYSISSIRFPKRNRMKVTYRSHNADYDYNYRKAQKQKQIDDILDKIARSGYDSLTK
                     EEKDILFKANNRNIN"
     assembly_gap    30057..30156
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<30157..31952)
                     /gene="mutL"
                     /locus_tag="PK049_04935"
     CDS             complement(<30157..31952)
                     /gene="mutL"
                     /locus_tag="PK049_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004297175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOK60934.1"
                     /translation="MADVIQLLPDSIANQIAAGEVVQRPASVVKELLENSIDAGARNI
                     NLIVREGGKGLIQVIDDGVGMSETDARLCFERHATSKIREAADLFNIRTMGFRGEALA
                     SIAAVAEVELRTRRAVDEVGTEVNISASELVSQQPVACSTGTQIAVKNLFFNVPARRK
                     FLKSDSVELKHIITEFQRVALAYPEVSFSLSHNGSELYRLPASGLKQRVIGLFGKTIN
                     QHLVDCSTQTSIVNIGGYIGKPEGAKKTAGEQFFFVNGRFMKNPFLHKAVMNAYNRLL
                     PPDTYPSYFIYFDIDPQSIDVNIHPTKTEIKFEDERMIWQILHAAVRESLGKFSVVPP
                     IDFDSAPGFDIPYLPKNAPVTFPTIDVDPTYNPFDEHNRNVRKPHSADKLRESATGWE
                     QLFTDLPQDPTPIQTRIETFESEVMLSKAPENTNRFYQFKGKYILTTVKSGLMVIDQK
                     RAHERILYEQYISGMQADINLKQRELFPQAIELLPADFELLMSKADELSRLGMEISNL
                     NHNTISVNSMPVSSSVTDPAKLVENLLAIMHENQALPFDDAKHRIALTMAKASAIGYG
                     KQLSQFEMQEIVDKLFACHEPNLTPDGKKIIV"
     gene            complement(31954..32232)
                     /locus_tag="PK049_04940"
     CDS             complement(31954..32232)
                     /locus_tag="PK049_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60935.1"
                     /translation="MAGFKFFSIPKPKGFRYKPIYYNEQKEEMREREERVRKELGLTD
                     KDKPFVPSIRGQFQRARKISRPSSRQSNIRLLIIIAILLLISYFVFFR"
BASE COUNT         9421 a         7415 c         6835 g         8454 t
ORIGIN      
        1 cctaccatgt tcattgtcca tgggacaatt ctcccattgc caaggttcat cttgctggag
       61 agaaagaact tgaacaggca atactgctag cacagcaagc cgagcgacca atggcagaac
      121 tggcagcctt tgaaaaacac gagatattaa tgcgtattgc tcagggaata aaggaaaacc
      181 gcaatagctt tgccaaaact cttgcccttg aggcttgcaa acccataaag ttggcctatg
      241 gcgagattga cagggccata caaactttca taattgcagc cgaggaagct aaaaggccac
      301 ctgcagagct catacgaatc gattggacac ctcaaggaaa aggtaaagag ggactggtaa
      361 agtgctttcc cgttggccta attgcaggta tagcaccatt caacttccct ctaaacctta
      421 gcgttcataa aatagctccc gccattgcgg ctggctgtcc aattatttta aagccagccc
      481 gttcaacacc cctatcggct ttaaaacttg ccgagattat tgactctacc ccactaccca
      541 agggtgcact ttcaatactg cccaccaacc gtgaggttgg tagcaaactg gtaaccgact
      601 ccaggtttgc cttgctatca tttaccggtt caccccaggt tggttggaat atgaaaaatc
      661 aggctggaca taaacgcgta gtgcttgaac ttggaggcaa cgcaggtgta gtagtaactc
      721 catcagccaa ccttgaacag gccgtttcaa agtgcctgag cggtgccttt gcctatgcag
      781 ggcaggtgtg cattcacacc caacgaattt ttgtacatca agcagttttc accgccttca
      841 ccgaaaagtt cctacaaggc acagcaaatc ttaagaatgg tcacccgctg gataccgaca
      901 ccgatatatc atcgatgatt gacgaggaaa atgcaataag agttgaggaa tgggtaaacg
      961 acgcaacgaa cgcgggtgct aaagtactac tcggggggaa acgcaaaggt tcctttttcc
     1021 agcccacggt tcttaccaac accacaaaca gcatgaaggt ttgcgcgcta gaagtttttg
     1081 gcccagtagt tacccttgaa ccctataccg attttcacga agcagttaat ctaataaacc
     1141 aatcagagtt tgggctacag gcaggtgttt tcacaaacct aattgacgaa atgaattttg
     1201 ctttcaatga gttacgagta ggcggagtaa tcattaacga tgtccccacc ttcagggtcg
     1261 accacatgcc ttacggtgga attaaaaact caggctttgg gcgcgaaggg gttaagtatg
     1321 ccatgctcga aatgctggag ccaaggcttt tagttaagaa tatactgtag tttaacacta
     1381 tttctcaatt cccaaaacat aacggtcaga tatttgctaa ttattggtat atttgcaccg
     1441 catctatgaa cgaaactacc atgcttttaa ccctgctaat caagggaatg attataggat
     1501 tggctgcaag cataccgctt ggaccaattg gggttatctg cgttcaacga accatcaata
     1561 agggtaagat tagcggtttt ctttctggtt taggagcagc cactgccgat acgctttttg
     1621 ctgccattgc ggggtttagc ctttcgttca ttatcagctt tcttgaagaa cgacaaacta
     1681 taattgaact tgttggcgga gttatagtag tttttttggg tttaaagatt ttttcaacaa
     1741 atccaatttc gcagcttaaa cggaacaaac ggaaaaagag caatttgctt gaggacttca
     1801 tgtcggttct tctgctaacc ggttccaatc cgcttgccgt tttctttttc attgcccttt
     1861 ttgccacagc aggggtaatt agccacgacc aaagtatatg ggtgaatcta atagcgctcg
     1921 ctggcgtttt ccttggcgga gctctttggt ggtatatcct tacatctacc gtaaactatt
     1981 ttcgcaggca ttttaggcta aagcagctct ggtggatcaa taagatttcg ggtgctgtaa
     2041 ttttcacgct gggaggactt gccattttaa acgtaatcca caaggtgttc ttttaacacc
     2101 acggttactc ctttgctttt cggcttatcg tattttctgt aaatttgaac taaacacaat
     2161 aggtttatat aaagttttta aattagccta ttaatttaaa atattgttat atttggattg
     2221 ttgtttaaac tgaaaatcat ttcagatgaa aatagccaat aaactcatag aggttttgtc
     2281 gtttggtgtt gatacaaaca aatcaaaaga ttttgccgat aagattactc agctaaacct
     2341 tattgctatt ccttcactgc tcattttcat aatcctagcc attacctcac ttaaagcatc
     2401 tttacctgtt gcaataatta atttttctac ttttatagta gctctggttt tactttttat
     2461 gcttttccgt tcaagccgct ttagcagtat tggtcttggc atttcctaca tttttgctat
     2521 aggcatactg gtaaatatac ttctgggttc aaacaacctt ttgctactag cagttacgat
     2581 gttgttacct ctaatgtttt tcagctctac cccattcaat aaagcatcgg tgacacttgt
     2641 agcatttgca gtactactcc ctattgttct atttatcctg cctgttattg gattacccgc
     2701 cgaaaatatt ttttatgtaa accgatattt cattagcatt ggatttattg tttacctaac
     2761 ctctgtgtcg ctgctatggt tcataagaac taaacagctg caagagttag aaataaaaag
     2821 cattgaactt aaaaatgaca ttaaaaaacg aactgagaac cttgccaagc tctcgcacga
     2881 cataagaaca ccattaaata acattgtcat aatctcaaac atactttcaa accaaatcca
     2941 ggaagagaaa ctcaaagata tgattgacac cattcaggct tcgtcaaata accttatgag
     3001 tactctgaac agtatgatag atctctccat aagcgagatg ccaaaggact ccgatgtact
     3061 tgtgccattt gaccttcaaa atacgcttaa caacacattc cgcctctttt caacccaatc
     3121 gggcactgtt agtttttcct ttaagtacga tgagcgtctt ccgaaaaacc tactgggcga
     3181 ccccgttaag ctaaagcaga tcttcctaaa cattgtggag agtatcatta aaaacaaaag
     3241 cagccaaaag gtaatacttg acattaagat caataaaatg caggaagctg gcgatggggt
     3301 tgacatttat accgaaatta aaacaaacac ccccttactc ctgcaccatg ccgatggtgt
     3361 atcgtcaaca accctaaccg atggctcaac cgccctgagt aaccaggtgt ttattgagat
     3421 gctcgaccta aaaattgcag ccaagcttat tcgcgaaaat ggtggcaagc taaatgttaa
     3481 gctaacgtcg caggactcac aaataaactt caacatcagg ctgcttaaag ttcccacagc
     3541 gcttaaagaa gaaatccagc aagccgaggg caaggaaaaa ggtatcaatg aaattgtacc
     3601 cgaaactcct gaagttagca aggttgaaaa gttacccaca acggatgttc gattagaaaa
     3661 tgccaatgtt ttgctggtgg aggacaacct gattaaccaa aagattgtat tactcagcct
     3721 taaaaagctg gttaaaaaca ttgatgtagc tgtaaacggg aaagaggcat tagataaatt
     3781 tggaaccagc aagtacgata ttattctgat ggatgttcaa atgccaatca tgaatggcta
     3841 cacggcagct aaaaagattc gggagattga ggcaagcaca aactcgcaca ccccaattat
     3901 agccataact gctaatgcac tcatgggcga cagggaagaa tgtttagccg ccggaatgga
     3961 cgattacatc agcaagcctt tccagattga ggtgcttatc cagaaaatgc gaaacctact
     4021 tgaggcaaat cgttagttat cgaaaaccct tgggttaaac aacgagtatc caaacgacaa
     4081 attcaaaaag aactgcttgc ccttatttct ggggtaatgc gataccgtta ttgttactgg
     4141 gcctaccgga gaactataaa ttactgaggc cgacatcaga tactccctgc ttttaaaggc
     4201 atcgccgtag taggctaaac catcgctatt agctaaaatg gctttgtaag gctgataaac
     4261 aaaagcatct attcggaaac tcacattact gccaaaatca acaaccggcg ataaaccaat
     4321 agcaacatac ttgcttgagc gaagttcctt taaaaacatt atccgcgaat gcggggttgg
     4381 cgagaactga ttcattgaca gaattgtagc ccagtagtta tcgaaaaacc gttgcgatga
     4441 ccagtagcca tccaggtaaa agccaagcca gaaactcttt ccaattagcc tatggtaact
     4501 ctcattgtgg aggtatgcag tgataaacga ttggtaattg tcaaattccg agagttgcat
     4561 ggctgttgtg cctggcttat gaatttcgtg tccgtagtac cccgatatcg atactagttg
     4621 atgtcgcccc cggttagcat actgcttatg gttcagggta tttttttcca gcctgattcc
     4681 ccccttaaag aaccggaagc gggttttatc ggggatatcg aatgagtagt agttctcggt
     4741 ttggtaatac tcatccatct tttcaccact ggttacaaac attgatgtgt tcagtgcgct
     4801 ggtatggcta aggcgcaacg caatagtgac aagggtctca ttctggatgg cataggcagg
     4861 ttttacatcc tcgaaaaagg gaaccgggtt accactatgg taatcgtaac ggttagctac
     4921 aagggttgac accagcgaga caggaattcg cgaagggaaa gttttcatgg ctgttagcct
     4981 tgcactactg tgaaattttc caaagtatat attgccgtac aaggtattag atgttctggt
     5041 taaccagtta tgggtaatag agaaaaaccc ttcattgcca ttatcggatg aaaggctcaa
     5101 acctaagcca aggctggggt gcctgttaag tatggcttgc attttgacat caaaaagtcc
     5161 gctattccta tcgtaaacga actggggttg aatgcgagca aacactttat cggacattaa
     5221 ccggtagtag ttaaccttaa acctatcata ggtaacaatg ctgaatttgc tcattaatac
     5281 tctgtcgata tacttttgct gcttggggtt caatcccaaa acgctgattt gtgatatgta
     5341 tagtggggga agtttctccc gaaactgttt gcgggcatga gcaaggctat cgagggatac
     5401 tctacggctt acctttgcct taatgctatc cattaagtta atcgccgact ggtaacctaa
     5461 gccaacaatc tcatcaaccc tgtcaaaatc ccaaaggtta acgtttggca aacgggtatc
     5521 aataaccatc ccaattgaat cgggaatatc atagttggtc atacctacaa tcattgcctc
     5581 gagctgttca aacggatcgt cgtccgaggg gattttagag ttattagcca ccttgctgcc
     5641 aataatgaaa tcgggcgaaa actcctttaa ggcctcaggc cagatgaagt tgttatagat
     5701 tccgccatcg aaaagcagta aactatcaac gtaaattggc ttatagtaaa gcgggaaagt
     5761 cattgaagtc ctgatacttc tccccaagtc gccatttcgg aagtaaacaa tgtttttacc
     5821 aaccacatcc gaggcattgc acctgaatgg cacaaagagt ttgttaaaat cgccacccga
     5881 taaggcggtg ccttgcgaaa agagctcatc aaaggcaata tccatcccaa ctgtaggtat
     5941 aaaactcgaa gccacctttg gtttaattcc cttggaagtt agcacgagtc ctacgctcag
     6001 attttcagca tcacttgaag agaaatcgtt aatgttatac ttaagcgatt cgtcaattat
     6061 ccctttactc caattgtaaa aatcgcgggt tttaaatagt ttaaccatct catcgggcga
     6121 atagcccatg gcgtaaagcc caccaataat ggcacccatt gatgttcccg acacgttgtc
     6181 gatagggatt ccgttctcct caagtgcttt aataactcca atgtgagcca agccctttgc
     6241 accacctccg cttaaaacca ggctcacctt ttgggcatta agccctggcg ctaatagaaa
     6301 cagaatgaaa acaacgatat attttgaacg gcacataggt gtaatcgatt tgtcaagcta
     6361 aaattaggaa tacttaattg tctaaacaat atcagaatac atattctgga atatctaaca
     6421 aaatttaata ctccagctaa ttggcatagc ataagaagga atgaaaccgt accagacgga
     6481 ataagtcagg ataaactgga taaaaaacaa cctacctact accaatagtc ctgaaaagca
     6541 taaagcaaat ggtgaaaagg gatttgttaa ttgttagccg ttatcaggta tagtctgatt
     6601 tttgacgtct gaattctgat tccttaatta agttgattga tgaaaatttt ctcaacgact
     6661 atttttctac ctttgcgtaa ctaaatcaaa taacagatga ctctcattaa atcgatatca
     6721 ggaatacggg ggacaattgg cggaagggtt ggtgaaaatc tcaccccagt tgacattgta
     6781 aggtttactg cggcctatgc ggagtggctg aaacaacgta ataatggtaa acgatatgga
     6841 gttgctgtag gtcgcgatgc ccgcatttca ggcgagatgg ttaacaatct ggtgattgga
     6901 acactcttgg gatgtggggt tgatgtcctt aaccttggac ttgccaccac acccacagtt
     6961 gaaatggcgg taatacatga aaaaaccgat gggggaatta ttctaactgc aagccacaat
     7021 ccccgtcagt ggaacgccct caaactactg aacgagaaag gcgaattcct atcggacacc
     7081 gatggccgaa aagtccttga aattgccgat agcgaaagct tttcgtttgc cgatatcgac
     7141 aaccttggca taaccgatga agagtactcc tttatagacg aacacattaa gcatattatc
     7201 aacctaaaat atgttgatac tgatgctatt gggaaagcaa actttagggt tgcaattgac
     7261 tgtgtcaact ccgttggcgg aatagcgcta ccacgactac taaaagcctt aggtgtaaag
     7321 gatatcattg aactgtatac cgaccccact ggcgattttc cgcacaaccc cgaaccccta
     7381 cccgaacatt taacggatat atcaaaagta ataaaggaac gcagagccga cgtgggcttt
     7441 gtggttgacc ccgatgtgga caggcttgcc attattaacg aagatggcac tatgtttggc
     7501 gaggagtaca ccctggttgc ttgtgccgat tatgtactga gccaaaaccc tggcaacacc
     7561 gtgtcgaacc tttcgagcac tgcagccctg cgcgatatta cccttaaata cggcaaggaa
     7621 tactttgcct ccgcagtggg cgaggttaac gtggttacaa aaatgaaaga ggtgaatgcc
     7681 attattggtg gcgagggtaa tggcggtgta atactcccag aactgcatta tggacgcgac
     7741 gcccttgttg gcattgccct attcctttcg caccttgcca aaagcagaat gaaatgcact
     7801 gaacttcgtc ggcaataccc tagctatttc atctcaaaaa acaaaataga acttaagcag
     7861 ggtttaaacc ccgatgccgt tctagaacga cttaaggaaa aattctcaac cgagaaaatc
     7921 aataccatcg atgggttgcg tatcgacttt gcggaaggat gggtacatat gcgcaaatcg
     7981 aacaccgaac caatcattcg gatatatgcc gaagcccaaa cggccgcgaa agcaaatgaa
     8041 ctcgctcaac aaataattaa ggaggccaca aagtaaagtt taggtatcct gtcaaacaag
     8101 aaactccggt tttcccggag tttcttgttg tttagaatgg taaatcatcg tcgggttggg
     8161 gaatttcagc atcgctttca gcagcaccca catcggttcc atgtgattca ttgtagttgt
     8221 ttggtttttc tccctgacca gtgcttggac caagaaagcg gaagttatcg caaagtatct
     8281 cagtggtata acgcttatta ccctccttgt cctcgtacga ggaattacgt atgcgccctt
     8341 caacgtaaag gagtttacca cttttaacat acttttcagc aatctcagcc aaaccacgcc
     8401 aaacaacaat gttgtgccat tcggtttgag ttaccttttt acccgactta tcggtgtagg
     8461 tctcattggt tgccaaagag aaacgggcaa cagttgctcc accttcaagg tgacgaacct
     8521 cagggtcctt gcctacgttt cctaccagga ttactttgtt tactgacatc gttactaatt
     8581 tttggttagg gtttataact aacccaattt aagtaaaaaa acgcacctat gcaattattt
     8641 ttttgttgaa aaatatttta gtgcaggttc agcagccatg tatgcatcaa tcactcgtgg
     8701 taaagagtaa ttgttgacat tgtcaattgg aacccttacg tactcatcgg caagcacgta
     8761 gttaggttta tcaacctcaa gtattaaaaa ggtagcccag atagtcctat ggctcaatat
     8821 atgacggact ggctgcgaga tattcaacac tttcaccttg gaacccgaca ggaagcttag
     8881 actattcaac atttcaatca actcatcggg tttcagtgct gccgaagttt cgtgcattgg
     8941 gaactcatac aacgagtgcc aaatatcctt ttgctcgcgc ctttttatat aggtataccc
     9001 tttgtgcttt accatgaagt agtaaaaaaa ccggtcagtg ggcggtttct ttttaccctt
     9061 tactggtagt gcagcaatca ggttattctg gaatgcatag cagtagtcgg caaaggggca
     9121 ttctgggcag cgtggcgatt ttggggtaca ctgcagcgca ccaaaatcaa tcatggcctg
     9181 gttaaaccgc ccaggttcag tacgggagat gagttcattt gccaatgcaa aaaactcctt
     9241 tttgccgctt gtggaatcga taggtgtaaa tattccaaaa atacgcgata gtattcggta
     9301 aacatttcca tcaaccgcag gcacagcctc atcaaaggca aacgatgcaa cagccgcagc
     9361 tgagtaagca cctaacccct taatccttaa aagttcacta taacttgtgg gcaaactccc
     9421 attgtacttt tccacaacct gaattgcgcc tgctttaaga tttcgggctc gtgtatagta
     9481 gccaaggccc tgccaaacct tcattacatc atctatatgg gcatcggcaa gcgactgaat
     9541 ggttggaaaa cgctcaataa agcggtagta atacgaaatg ccctgtacaa ccctagtttg
     9601 ctggagtatt acctccgaca cccaaatata gtaagggttg gaagtgttac gccacggcaa
     9661 atcgcgcgca tttgccatat accacttcaa aagtattgaa ctaaaatcca tgctatcaaa
     9721 tcaaaagaaa aaaaaatctt gtcaaaaata accttaacca ttttatatat cgatacaaaa
     9781 catgtatatt tgcaatccaa atttgaaagt aattgtctaa ttcattgata actaaagatt
     9841 ttcaaaaatg acaaaggctg atatcgtaaa cgagatttcc aagagtactg gaatcgagaa
     9901 agtagcagtt cagaaaaccg ttgaggcttt catggaagcc gtaaaggatt ccttggtaaa
     9961 ggaaaagaat gtttacctgc gtggcttcgg cagctttatc gttaaaaagc gtgctaaaaa
    10021 aaccgctcgt aacatctcaa agaacaccac catcattatt cctgagcact tcattcctgc
    10081 ttttaaacct tcaaagtcgt ttgtaaacaa ggttaagaac cacgtaaaga aatagtttag
    10141 tactaacaat caaaatttac cacaatgcca agcggaaaga agagaaaaag acacaaaatg
    10201 gcaacccaca agcgtaaaaa acgtctacgt aagaatcgcc ataaaaagaa aaatagataa
    10261 ccatttgcta atcagcatgg tgacataagc aacctaaagt gcctcaaggt gctttaggtt
    10321 gcattgttta atcgctaacc acaaaataca cgtaaaaagt gaataccgag ttaatcatcg
    10381 atgttcgtga aactgaagtt gcgattgccc tgctcgaaaa caagcagctg gtggagctca
    10441 acaaggagcg gagcaacgtt cagttctcgg ttggcgacat ctatctcggt aaagtaaaaa
    10501 aaattatgcc cgggttaaat gcagcctttg tagatgtggg ctacgaaaag gacgcattcc
    10561 ttcattacct cgaccttggg ccacagttta aaacactcca gaagtacgtt caggatcact
    10621 actcgcgtaa aaacaaaacc attcccatct caaagtacgg gatgttgccc gacattgaca
    10681 agagtgggaa aataaccgat gtgcttagca ccggacagcc ggtaatggtg caaattgcca
    10741 aggagcccat ttcaaccaaa gggccaaggc tcacctctga aatatcaatt gccggccgga
    10801 atatcgttct tgtacccttt aacgataagg tttcaatctc gcaaaaaatt actgcccccg
    10861 aggagcgcaa gcgactaaaa aggctaatgg aaagtattat tccaaagaat tttggggtta
    10921 ttgtccgtac cgccgccgag ggcaaaaagg ttgcagttct cgatgcagag ctgcgcagct
    10981 taattaaaag gtgggaaatc accgttgaaa aacttaagaa tccgggttat ccggtactgc
    11041 ttaccagcga aattaaacga acatcggcta tcctgaggga tatgcttaac ggatcgttca
    11101 acagcattca tgtgaacgac gagaacgttt acgaagaaat acgcgagtac attgagagca
    11161 ttgcacccga aaaagaaaaa attgtaaagc tttacaacgg aaatgtgccc atatttgacc
    11221 agtttggtgt tgaaaagcaa attaaatcgc tatttggccg gacggtttca attaaaagcg
    11281 gggcatacct cattgttgag catactgagg cgcttcatgt gattgatgtg aacagcggca
    11341 accgctccaa atcgtcgaac gaccaggaaa caaacgccct tgaggttaac ctggctgcta
    11401 ccgacgagat tgcccgtcaa ctcaggcttc gcgatatggg cggtataatc gttgtcgatt
    11461 tcatcgacat gcatagcaac gagaacaagc aaatggtgta cgagcgcatg aaggagctca
    11521 tgagccgcga ccgtgccaag cacaatattc tgccgctaac aaagtttggg cttatgcaaa
    11581 tcacccgcca acgggttcgg cctgaaatgc acattgacac cagcgagaaa tgtcctgctt
    11641 gtaacggaag cggaaaggtt accgccacaa tcctgctcga tgagcaaatc gagaaccagc
    11701 tatcgctaat atgcgcaagc aaaaagtacc gctccgttac cattaagcta cacccttaca
    11761 ttgctgctta catcaaaaag ggatttcttt ctaaacgaat taagtgggct ttaaggtata
    11821 aatgcgccat taacctatta cccatgtcgt cgtaccattt tctggagtac catttctttg
    11881 ataagcacga taacgagata gaactttagc caaccaagtg gggtgtcaag gcatgacacc
    11941 ctttttttta ttttaaaaac cacaactatc caactaaagt ttataagaat tagtaaaatc
    12001 cttacttaac atatccccag tgaggaagga aattttaggg ttgtttttaa acaactcttc
    12061 atcgcctatg gcgaaacgta atcctattac cacgaagcag ttaaactgtt gtaaaagtat
    12121 agaaaagaag gtactttaat taccatgtgg aggttaaggg tttttatggg acagtagtga
    12181 ataaaaaaca ttaaacattt ttgtaacttt gttaaacata ttatttaaaa aactattctt
    12241 taactaacta ataattaaca taatgaagaa agcattaatg gtattcattc cactcatggc
    12301 aatgtttaag atgggtgatg cgcagaactt atcatttggg gctgaattaa gctcaaattt
    12361 tcacttagaa tcaatattac cggaagttgg attaaaacta aatgtaccgt taagtaagca
    12421 cagccaattt gaaaccggtt tattttacca aatcaggaaa aacgatggcg catttagtat
    12481 taacaacgat ctttacattt ttgatgtaaa tgaatggtac ctagttgtac cattaatgta
    12541 taaattctca agccagatca taaacctatc atttggaatt aattacaact attatgtagg
    12601 atggggtgtt gcttcagtta gcgatgaaaa tgtacaaatc aacaactaca acagagaaga
    12661 tacttacctt tttggcgttt agggcgcact atcaaaatcg ttaagactga acaaccaatt
    12721 ttcaatagaa cctattatta aagtctctgt aatggtttac ccatatagcg atgtacttgc
    12781 aggatttggt tttgctttgg tttacaacaa gatagcccaa taataaaact tgaggaataa
    12841 ttggattaaa agcagggtgt cagtaaaata ccgacaccct ttttttatcc cattttttta
    12901 aactatttgt caaaaaatga gttgtaagta gtaataattt acttgtaaat ttcagggtac
    12961 gaaccaaaat tcaaaactat gaaaagaata tcatacattt taatgcttag catattagca
    13021 tttggaatgg ctgcatgcga agacgatgag agcggcgact acgacaactc cgttaccata
    13081 agcggaagcg ttaccattga tggcaaaacc tactccagcc caacctttaa catgggtaca
    13141 tccatggagg tagaaggtta tacataccta acaaataatc ctactgcgca aatctactac
    13201 acagccattt acccaaaaac tgagatggtt gatgctggta acgggttaat ggcgcactac
    13261 tattttgtgc ttgaggatga ccaacccggt actgcgagtt ttactggcat gatcatgttc
    13321 cataaaaacg acagctttgt ttttgggctt tggtcacagg atgttgatgt aacaattgac
    13381 aagatagacc ccgttggcgg ctttattgaa gctacctaca atggaacttt caccgatgaa
    13441 gggaacacac caaaaacata taccgtaacc gggaaaatca aggctaaaag agtatcacaa
    13501 ccaattattg ataaataata aaaaaccggc aaatgccggt ttttttattc caccactatc
    13561 tcatcggcaa aaagccatgt tggcgaaccc gatgccggat gccactcagg gcatgggcca
    13621 ggattatggg ctacaaaacg cacgtaacgc gccatgatat tatttactac aaaagcatta
    13681 aacctataaa ccagcacagg ttgacgtttt atgttttcag gacaatccat ttgagcgatt
    13741 tgctcaaagg atatactatc cgctgaaacg aaaacatcta cccggtttgg caggaatatc
    13801 catgagagaa tgttttgtaa aaaacctact gaaaaatttt ttattggttg atttgcgcca
    13861 aggtcaatgg tagctaccat atcgtgcccc tgaacggctt gccatatgcc atcgttatac
    13921 ttgggtgttc ccaaccggcc atcaacaaga gcctgcttac tgcctcccaa atacttttcg
    13981 gcaggaacaa aaccaagctc tatgggttta cctaaacttt tcgataccac aaatgatcgt
    14041 tcaaaaacat cacctacacg attgccattg ttaaatgcag cggcccttaa ggtagttgac
    14101 gaggttaact caattggttt acaatagcgt gtgctattca cggttggttc atcgccattt
    14161 gtggtgtatc gtatagtaag attatcctgc cctttaataa gttcaacgaa aatggacttt
    14221 ttatcatcac ctatacttga tttaaactca actgccgatt gctcaaaacc ataggttaca
    14281 cccaatgcct ctaatcggtg ataatggtta cgaacccggt ttagaaactc gttgtagttg
    14341 cgttcccgtg gataagtcca caaaacctct gcaatggcaa gtatacgtgg aaataccttt
    14401 gaatcgactg tttcctgagg ggcatactca gtccacatgt tgcactcgcc accaagaacc
    14461 agctgaatct ttgctgaatc gacctgcgcg ggtacggggt caaagctgta aaccttttgc
    14521 aggttggtaa cctcaacggg ataatcgaag tagcaatggc ttgttggcga cacaataacc
    14581 ggtgcgttgg cgcttagggc tttcgtagcc ctatcgatac cacgccatgc ctgaaccgta
    14641 gcattgtgcg gcctgccccc ctccagtatc tcatcccagc caataatggt tttgcctttg
    14701 ccctccaaaa attttgctat ttggctaata aagtaacgct gtaattcctc gtagttgctc
    14761 aacccctcgc tttttagcct tgctttacat tttgggcaat tctcccaacg atacttaggc
    14821 gcctcatcgc ctccaatgtg aatgtatggc gaagggaaaa gttccacaac ctcagtaaga
    14881 acgtcctgca aaaagatgaa ggtagaatcg ttcccggcac agtaaatatc cttaaaaacg
    14941 ccccagttgg tttcaacctt taacggttct cccgtgcacg aaaggttggg ataggccgcc
    15001 aaagcggcta ctgaatgccc gggcatttca atttctggga caaccatcac atggcgtttt
    15061 gcggcgtatg aaaccacctc gcgaatatcg tcctgggtgt agaaaccccc gtaaaccgtt
    15121 ccatcggcat tggttcgcca agcaccaatc tcggttaacc ttggatactt ttttatctca
    15181 attcgccaac cctggtcttc ggtgaggtgc cagtgcagaa cgttcatttt atggtatgcc
    15241 aacaggtcga tgtaacgctt cacaaaatcc ttggtcataa aatggcggca acagtcaagg
    15301 ttgagccccc gccaggggaa gcgcggctta tcaataatac taagacaagg tactgttacc
    15361 gatttttgcc taaaatcgtt tttctcgaaa ttggcaggca tcagctgcct gagggtttgt
    15421 atggcataga aaaagccagc agactcattt gctttaatta cgatatgttt gggcccaata
    15481 cttaacatgt acccctcagc acctaaacta tcggggcaac cggttctagt aaaaacgatg
    15541 ctattcgtgg aatcgactcc cacttcagca ttaaaagcac agcctacacc tttctggttc
    15601 agcagctcaa caaggtaaat tgcattattg aaagcaatgc tatcggcgct atcggctctt
    15661 actaaagttt tgctgttaat agtaaaatcg ccatcaacct gtttaatact tactggcttt
    15721 ggtataatgt ttacatcgct attcctgctg cacgacagga gcgctgtaag cgctagagtt
    15781 aacatcacca atttgcgcat ggcataaact ttttacttta tggaaatttc atcgataaaa
    15841 agccacgagg gttcgccggc actctcgtgc cacgaaggca gtgggccaaa tgcctttgca
    15901 aacactttta caaatcgggc ttttgtatca acattaactg taaagtcctt aatggtaggt
    15961 cccatctggt tttcaggtat atcgctttta acagtcccaa ccacaacaaa gttttgccca
    16021 tcggtcgata ccgaaaaact tacctcgcgc ggcatcatta tccaaggact tgcatcctga
    16081 agaaaacctg cagaaagttg ctttactggc tttacctcac ccaaatcgac aatggcctca
    16141 aaatcctgcc cctgatagcc ttgccacccg ccaatcctaa agttcttgct tcctctcacc
    16201 ccatcaacca ggcccaaatc gccaccggcc gtatagagtg agctgtattt tgatttgagt
    16261 tgaacattag caatgctgtt tacctttctg aatacccctt ccagaatggc acttgtttgc
    16321 ccattgggca aaatagcaaa ggtgcgaatc tttttgcttt ctgtaaggtt tatcttattg
    16381 ccctgaatcc aattggtttg aggcaggggt ttatccactg gctcaactgc gtaaaaaatt
    16441 tgagcatcgg gctgaaccga aactatggaa actgttaagg tatcggtaaa tacatcagat
    16501 gcagcaacca aaaatggcgc aattgttatt agattgtcat taattgatgt gctaggcaaa
    16561 tcagcaggct gagtacccca ggtatcgctt ggtgtactgg ccatggttaa aaccaactcg
    16621 ccaccagcca ttatctcggc ttggtcaatc caactccttg taatgggatt cccgttaagc
    16681 gtagcttttt gaatgtagat gttttggttg ttaagatttt cggctatcaa cgtaaatttt
    16741 ttaccattct caagattaat tgtgattttt gggaatagtg gtgaaccaat tgcatactgg
    16801 gttgaaccgg ggcagagcgg ataaaaaccc atggcgctca tgatgtacca agcactcatc
    16861 tgaccgcagt cctcgttgcc gcagatacca tcgggcttgt gactatacat ctccttcata
    16921 atccgtttaa caagctgttg tgttttccag ggtttaccca catagttgta taggtatgcc
    16981 atatggtggc ttggctcatt accatgggcg tattgtccta ttaaccctgt tatatcaacc
    17041 tgatttcgac cggcaagctg cgagggcgca ttaaagagct catcgagttt tgcctcgaac
    17101 ttatccattc ccccatgcaa ttttatcaaa cccgtaacat cttgtggcac atagaagctg
    17161 tactgccaac tgttggcctc agtgaaatgc tggtcaacct cggcagggtt gaatttctgc
    17221 ttaaaacctc cgtttaggcg tggccgcatt aaacgggtat cggggtcgta taggtttttg
    17281 tacgattgcg ccctcttaat aaatgtgttg taatcatcat acttccctaa accttttgcc
    17341 atttgggcaa tacaccaatc gtcgtatgcg tactcaagtg ttttcgatac cgactcgtgc
    17401 tccatatcgc caggaataaa accataacgt ttgtagtact gcaatccaaa gtgattaaga
    17461 tttgcgctgt gcttcatggc ctgtaaggca tactcggcat caaagccacg tataccctta
    17521 atccaagcat cggtaattac cgagacggag tggtagccta tcatacagtc ggtttcgtta
    17581 gctgcaagct cccaaaccgg aagcctacca ccataacgga agtgcgataa gaacgtattg
    17641 atgtaatcgg atgtgcgttt ggtattaata atggtcatta acggatgcca tgcacggtag
    17701 gtatcccaga tggaaaatac ggtgtagggt gtaaaaccat cggcgtaacg aacattacca
    17761 tccatggtgc ggtacaatcc attcacatcg gaaaagatat ttggaacaac catggcatgg
    17821 tacatggctg tgtaaaaggt ggttagctcc tcgggagtac ccccgtcaac ctgaattttt
    17881 gataattcct cgttccactt attgcgtgct gcctctacaa cgccatcaaa atcccagccg
    17941 ggaacctcgg cctcaagatt cttgagagcg ccctcctcgc taacaaagga tatacccacc
    18001 tttaccagta aaactttgtt tttcagcttt gggaactgta cccatgcttt cagcttggtt
    18061 ccatgaacct caaccgtgcc atcctcgtta ttaagggtat tcatttcccc gctatcgtcg
    18121 gtaaacttgg ggcccaatgc aaagggttct gagaatcggg ccacaaagta gattttttgg
    18181 tctttagccc aatgcgatga acggcggtag ccacgaatct cgtttttccc cgtaacctta
    18241 aaccatgcct ccagcacctt atcgcggtgc gaaaggtcaa tgaccatggc tggttcgttt
    18301 ggtttggtat aggtgtacct atgaaaccca acccttgctg ttgtagttag ctcagtcttc
    18361 accttgtatt tatccaggaa taccgagtag tagccagcct ttgcctgctc attctttttg
    18421 ttaaatggcg atgagtactt gctattctca aaaatgggcg atttcccggt aactggtgca
    18481 aacagaatat caccatagtc cgatgcaccg gtaccgctca ggtgtgtgtg cgaaaatccg
    18541 taaataaccc tatcggagta gtggtaacct gaacatccat cccagcccgt taagcgggta
    18601 tcggggctta gctgcaccat tccaaaggga accgtagccc cggggtaagt gtggccgtga
    18661 aaatcggtgc caatgaaagg gtttacataa tcggcagggc ctttctgcgc ccaaaggctt
    18721 acagcctgca taagtaccgt tactaatgcc agtggtttca tactatttag gtttatgatt
    18781 tattccgatg tttacctttt tatctctctt attcgttgaa ccatacccat acccagaata
    18841 tccttatggg gcgacctagt gaagtagtaa acgctctcaa agcgcgtttc attatcgatg
    18901 ctaacagtta atttcaggta gatatcgttg cgtacaacta gttcattggc aaacggtatt
    18961 ctaccaacct caacagcaac attaggttgt aaagtcaagt tccggttaaa ttgatttaat
    19021 tcatttccat tgatgtcaaa aactttagcg gaaatgcttg cctgtatggg ctttatacta
    19081 tcggatacgg cttttaccac aaccaatgta tcttccaaat gggcaccagc aataacaggt
    19141 ttatagagat tttgcacgta atactgaacc cgtttccacc tctcagcata gtcgatacct
    19201 gaccacgata ccacaggcca gcagtcgttc agctgccagt acagcgtacc catgcagtag
    19261 ggcgatgcca acctatgcgc ctcaatggcg tgcgcataac cctgagcctg aaccaattga
    19321 ctataatcga tccactccag caaatcgttg ggtttaagcc cctcgcgttc catgtactcg
    19381 tttatggttt gatagcctac cggatgtttc tggtggcaaa gcatttgcgc cgagtcgggc
    19441 agattattcc ggtcgttaaa cattcgtaga gtttcttccg aaggggctcc ctgaaatcca
    19501 tactcactca taaagcgagg tattctttga gcgtaccgct caaaaggctc cctaccccac
    19561 caaacgcccc agtagtggga atcgccatgc gtggtgcttt cagctcttcc ccaaccaaac
    19621 atgggcgacg atggccagta gcttctggtg gaatcgtaac gcataacaac ctcaggcaaa
    19681 agatggagga acagctgaac gtaaccgttc cataccgaat cggctgctcc cgtataatcg
    19741 cttagctgct tttgccagcc ccaattatgc cacccctcgt ccacctcatt gttgccacac
    19801 cataacgcaa ggcttgtgtg cttgcgcagt cgctttacct gttcaacagc ttcctccttt
    19861 acattggtaa ggaaagcaga actgtaagga tacattgagc aggcaaacat aaaatcgtgc
    19921 caaaccagaa tgcccttacg ggtgcactcc tccataaagg catcgggtgc ataaacacct
    19981 ccaccccaaa cccttaacat gttaaaatta ctctgggcag caaaatcaac ctgttgaacc
    20041 caagccgaat ccgcaatgct atgcacaaag aagtcgggcg ggataaggtt tgcccccttg
    20101 gcaaacatcg gcaagccgtt tactaaaaag taaaacgatt gcccagaact atcgggctcc
    20161 gatatcaacc ttacggttct aattcccaca tcatgcactt tatcgtaaac caactttcta
    20221 tcttcaaaaa attgtactct aaatcgattt agatgctgct ctcccatacc gtttggccac
    20281 cagagtttgg ggttatcaat ataaaaacgt gcgcctacct catggcgttt aagccgcatg
    20341 gctgtatcaa ggtaaatctt gtcagtataa acatcggtta ctgttagcct taattgggct
    20401 ttggtatcac catctatatc gaaatcgata gccattaagg ctttttgatt gctaacagaa
    20461 attgtagata ttgataggtt tgagactcgc actttgggag ttatccttaa gtaaaccggt
    20521 ttccatatac cacaggtaac gaatcggggg ccccagtccc aaccaaaatg gtaagcagcc
    20581 ttacggacat aggcccattc tccgccaggc aacctgtaac tcccacgccc ggcaatgctg
    20641 tcgaccatcc tgaccgccga acgaaaaact acccttagct catttttccc tttcctaagg
    20701 aactttgagc aaggaaccac ccaccgcctg aacatgttat cggccgtaag cacttttacc
    20761 ccgttcaggt aaacatcggc ataggtatca agcccttcaa acacgagctc aacatccctg
    20821 atcgaactaa aggttttagg caaggcaaag actttgcggt actcccaatc ggcactatca
    20881 atccaagcca ggtgcatatt gttacagcca aggtatggat tgccaatcat accattggaa
    20941 tgaaggtggg tgtgaactgc cccaggaacg gttgcaggca gccaaccatc actggagttt
    21001 gccctcctga attgccactg tccattgagg ggaaactctt gctgcgacaa ggcagcaaaa
    21061 gccatcaccc ataaaaccag aaccgatata gcctttttca ccttttatag tgttttcagt
    21121 aacccttatt cccttgtcat gctgagctca ccgaagcatc tctttgcctt tttcctcttc
    21181 aaccttttgc ctgagctccc tcgactacgc tcgggatgac aagtgaagtt gttcgttgat
    21241 agttgttcgt tgattgtaag agcataagaa actacttatc cccagccatt ttgtgcgttc
    21301 tgtaccgcca atagtttaaa gttaaacatc tcatcattat tctgaaagtg agatcattcg
    21361 cttcacttgg gcgacaagaa gcatcaacgc tttttatcaa tcattgcaag agcatacacg
    21421 taagccccca cagccaccgc atggcttgtg ccaaggcgcg ataaacctac accaatccgt
    21481 ttcattgggt cgtactcaac tgtacgattt gaaaagggaa cgcttacccg ctgctgctga
    21541 cccgacagga acctggctcg ctgcgcttca tcctcaatat cgaacgcctt aacctcaagc
    21601 ctatcgaccc ttgctccatc ggtgcgggaa atagttccgt tgagctggct gagcatggct
    21661 ggggcaaaat acttccatgc attagacann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21781 nnnnnnnngt tccgttgagc tggctgagca tggctggggc aaaatacttc catgcattag
    21841 acaaacctcc accaatcacc accaacgaat cggtcagggt gacggcgtta gcaatggcat
    21901 cgcctaccac tacgcctagg gtttgaaagg ctaaccttgc agccacaagg tcgccctcct
    21961 gcttgccctc aagcacctcg tagatatcct ttggtgttat cggcttgcca tgactatgct
    22021 tgctgtacga atggtaaacg cgttgaacag cccttatgct tgctccctcc tcggcaaagc
    22081 agtcgggatc gatagcgtta cgaactaacc aaatctcggc gccagccgaa ttgtcgccaa
    22141 tgaaaagatt tccatcgacc acaagccctg ctccaaatcc ggttcctaac gtaatcccca
    22201 gcaaatgccg gtagcgctta ttacttccac tctcggcaag aagctggttt acgtatggta
    22261 aaaatccagc aatagcctcg ccgtaggcaa acaaatcgcc atcgttattg ataaaaacgg
    22321 gtaagtgaaa cctatcctca agcatgggtc ccaaggctat accgccacgg aaagcaggca
    22381 gattgcccaa atcgccaatt ataccattgg gataatcggc cgggccaggg aatgcaaaac
    22441 tgatagctac aggcttttcc gttaaaccat tcatcaaact gctaaagcca tcgataatgc
    22501 ctgaaaggca tttatcgaga ttatcgccat tgctcggaag cgtaatgggt tcaccaatac
    22561 gctccatccc tctaatggcg ctgaacacaa agtttgtacc gccggcatcg agggtaagca
    22621 ccactcgatt atcgttccta tagtccatta cgattgtgtt ttaggtttaa ccatggtgag
    22681 cagcgcaatg aacgcaatac cagccaaggg aacaataaac ccaagcctaa cgctggttgc
    22741 atcggcaagc agacccataa gcggtggaat aaaagcgcca ccagcaatgg cggttaccat
    22801 aagtcccgat atctcattag cccgctcagg catggaatcg actgtaattg agaatatcaa
    22861 cgggaaaata ttggcaaagc caagacctgt gataaacaca ccggccaggg ttacaacctg
    22921 attatcaaca aaaatcaaca aaagacctaa taccgacagg gcaacggtca tgcgaaagaa
    22981 actatggggt tttacccacg atagtattac tgagcccaaa aacctaccgg ttagaatggc
    23041 aatgaagaaa aatgcattac ccagcaagcc ccatgtcttt aaatcgatgc cgtaatgtga
    23101 gctgagcagg ataggaactc ctgaactcaa gcatatctca ataccaacgt aaacaaaaat
    23161 tcctaaaacc ataagtagaa caaacctgtt tgaaagcaag ctaaagcagc tagcaagagt
    23221 agcaggctcc ttgtcggcct taacctcctg tatacgggta aacgcaataa ttataagggt
    23281 gattaaaagc gatgccacat aaattgggaa aagtaccttc cagtccatcc ccatccactt
    23341 ggcggctgca aagggaataa gcgaaccgga aagcgagccg atagccttaa tgaattgccc
    23401 aaaactcagg tttcgtgagt attttccggg ctcggaaaca tcgcgcataa taggattacc
    23461 ggcaacctgc agaattgcgg cacccgcacc aagcagcagg atggttagca gaaaaagtgc
    23521 atagctactt agcccaaaaa gcggaaggac aaggccaacc aacgcaatgg aaagacccag
    23581 catcaaaaca aactttttac cagttcggtc ctgaaccaaa cccatgggta ccgataaaag
    23641 cccaaacata ataaatccca tgaaggcaat aagctgcgcc tcaaagttcg ataaagcaaa
    23701 ctgctccttg gctaatccca caaaggggcc tactgcatcg ccaaaaccca tacacaggaa
    23761 ggcaagcaaa atggcaaatg ttttacgatt cataaggtgt gattttagtt tgatattcta
    23821 tttcgattta ttctgcgatg attggcgttc gttaagcttt acaggaagcc ttatcacctc
    23881 atggctcaaa tagctttccc catccattag ctggctcaac acttcaaatg ccctgctgca
    23941 catctgggca atgggttgct caatggcagt gattgtgggg ctgataaagc taaagtacgg
    24001 taaatcgtcg aagcatacca ttgccatatc gctggggatt ttgacgttgg aagcggccag
    24061 tgagtgcagc acagcggtgg ctatgttatt atttactgca aacaggcaat cgggcttaaa
    24121 aggaagcgac ttaacgttta acagtagctc atccacatcg gcctggatgg cgttgaaccc
    24181 cacctcgcga accaagcatg gcaaattgtg ttccttgaac acacacaaaa atccatcgat
    24241 acgttcagcc accgaggaaa tgtgacttgt ggacagtccc agcacgaggg gttttttata
    24301 tccctgctcc accaggtgat gggcaagcat ggcgccccct gacctgttat cggccacaac
    24361 ggcgggtagc gtgccgctac ccaaatgcct atcgataagc accagcggtg taccgctgtc
    24421 gtaaacagcc tttaaccact ctccctcacg ctgcgacgtt gacaccacaa gcccatcgac
    24481 ctgacggttg cgtagcatgg taattagccg ctgctccttt tcaacttcct cgtcggtgct
    24541 gcaaatgata acgctatacc cctgcgcaga ggctaagtcc tcgaacatgc gggccatttg
    24601 tgcataaaaa gcatttgaga tatcggacac aatcagccca acggtttcgg ttttcccggt
    24661 gcgcaggctt cgggcggtac gatttggcct atagttcaac tcctttaccg ccgcaaaaac
    24721 cttatcgcag gtttgtttcg aaataccgta ctgctgggct ttcccgttca gcaccagcga
    24781 taccaaagtc tttgataccc caacatgctg ggcaatatgg tagagcgtaa cacggtttgc
    24841 catacaaaat gtttaggttg ggtaaaggta taaaaaaatc taaatcgatt tagtaatttg
    24901 atagctaaaa ttaaagggat ggtatcacct tttaataacg agaccattaa caccccgcaa
    24961 caatcgacca aactgttaaa ttttaggcac tcaggatgaa aaagttggta caattaatta
    25021 cgcacaaccc acacaaaaaa atgcgcttta ccgattgtga atttttggtt tttttataca
    25081 ttcacatttg aataagtttt actacttggt ttaaccccct attctttgga cagatttata
    25141 gaaatgattt aggacacttt gattgtcatc ctgagcgaag cgaaggatcc ccccttcaga
    25201 aggttaataa aatgtctctc tcaattcaga gcgggataag ccgcctaacc tagcaaaaca
    25261 gatttattgg gattaaacgt gtttgaatga cactctctac accttggagt tgtttcgcta
    25321 cgctcaacat gacaaagtga atgagggggt caatgcgttg tcattccgaa ctagtcgagg
    25381 aatctcatta aatcaaggaa tgaagctaca aagagatgct tcggctagct cagcatgaca
    25441 agggagcaga aaggaagata gggtttctgg cattccgtgc aagtcgagga atctcgatat
    25501 atttaagatt taagaactca ctagttagca cacgtcttta gacgcacata taaatcgccc
    25561 tgaaagggca aatcacatta gccctgggtt ttaacccagg gtattgaggt attcaaatta
    25621 tttggcgatg cacgtattgt atattccaaa gagcaattct gatgcgttgc atcgcaacag
    25681 aaaaatcaga acaaagagcg atttacacgt tctttcttgt cttgatacaa gaaagaacca
    25741 aagaaaatca aggctgaaaa gcccgacccg atgggtaccc cgcgggtgga actgccacgc
    25801 gatactattc gccacgccta cggcgtgact catttggtat cgcttactaa gcccaacgca
    25861 acgttccacc cacgacccat tgccggttca ggcctttatg ccactgctta agtcattcca
    25921 tgctggttta ggaattttat caattaaaag gggaatgtaa gtaaaaagag atgcttcgac
    25981 tgggctcagc atggcaactg tctcaagaat cgagcaaaag caagtatcac agagttacac
    26041 ggtgttactc ggtgttacac agagcgtgtg acgtgatact cacgagcaac gaaacacgaa
    26101 caacgaacaa ccatcacccc taccggttaa tttgggttaa agagttgggc aagggtactt
    26161 gcccatttac tgaatgtgct atatttgaga aaaatctatc tgttttgaga aagtgcttcc
    26221 ttctgttctg gttactggca ataattgctg gtaatagcaa ccttagagcg caggtattac
    26281 tcccggtgcc cgactcgttg cgcgttatca atttcagggt tacggattac tactcccatg
    26341 agccaattgg gcttgcccat gttatgaata tcacccagaa aaagggttgt atctccgacc
    26401 tgctgggtta ctttagcatt ccatttaagc tgggcgatag ccttagcatt actgctatag
    26461 gctaccacac caagcatgtg ctgaactggg gacagtttaa caaggatacc cttttttacg
    26521 agctaaccct taccccaaag gtttaccaga ttgaagaggt taaaatatcg cgtttcagca
    26581 cctacgaccg atttctacgc gagtttgcca acctaaagct gacaaaaaca agggtaacgg
    26641 agcagcagga ggtaatacag ctatacttcc ttaagatcac caagggctta aacctgcgta
    26701 acctaccaca gccaaccatg gggttaagct ttggcaagga ctggtaccag aagcaaaacg
    26761 aaaaggttgc tgaggccatt gaaaaggata gaaaaaagcg aatagccgac cgaaaattta
    26821 atgcgggtat agttagcagc ctaaccggtt taaccggaaa tgaactgcag gaatttatgg
    26881 aatacctgaa gttcgacgag aactatatac taacctcaac cgattacgaa atacgtgaac
    26941 ggatacttga taggttcaag gaatttgaaa cgattaaaaa agggaaaaat aacaccaaaa
    27001 agtaaacagt atggcaatgc aggatgtaac cactccaagc catttagaca ttgagaatgc
    27061 ttttaatctt attgcgaaac acataaatag cacgccggtt ctaacatcca ggagtgtaaa
    27121 caccatatta gggtgtaacg cttaccttaa atgcgagaac tttcagcggg taggagcctt
    27181 taagttccgt ggggccaccc atgccattct gcgtttaggt agcgatgagg ctcacaacgg
    27241 tgttatcacc catagctcag gcaaccatgc tgcagcgttg gcattggctg cacggcaaag
    27301 gggaataccg gcatacattg ttatgcctga aaatgctccc gaaatcaaaa ggaaagctgt
    27361 tgcagggtat ggtgggataa tcaccttttg ccctccgacg caagccgata gggagcaaac
    27421 catgctccgc ataaagtccc aaaccgaagc cacattcatt cacccctacg ataactttaa
    27481 cgtggtgtgc ggacaaggaa cagcagcact ggaacttatg cagcaggaac ctgagctcga
    27541 tattgttatt gcgcctgttg ggggtggagg attacttagc ggaactgcca catttatcaa
    27601 agggcggttc ccgcatgtta aggtttatgg tgctgagcca aagaatgccg acgatgccta
    27661 ccgctcgctc cgagaccata ccatttaccc ctcggtaaaa ccccaaacca tagccgatgg
    27721 gttactcaca tcgctttcgc ccctaaccta caccattctt agccagcact gcgacggtat
    27781 actcactgta tcagaacaat caattattga ggccatgcgc atggtgtggg agcgcatgaa
    27841 gattattatt gagccatcgt cggctgtgcc ggtggcggcc atcatggaga atgctcaact
    27901 tttccgctac aaacgtgttg gcgtaataat ttcagggggt aatgtggatt taagccatct
    27961 gccctggcag ggctaacggc ttcactgtag ttgcagttca acaacaaccg ggaaatggtc
    28021 agagtacctc agccgtgggc tttggtaggt tacggttcgg aactgtctac tccgtaagac
    28081 ataatcgatt cgatacgagg gccacaggcc gctgaaggtg actacaaccc cgctacctgc
    28141 atctttaaat gtatcgtgta gggaagccgt tagggtatgg taggtgtacg atacgggcga
    28201 atcgttaaaa tcgccgcaaa taataatggg gtaacggcaa gtacgcatgt gttcaaccac
    28261 catattcacc tggcgggccc gctttgtaaa ggcaacgcct aatcggctga gtaaatcctt
    28321 tagctcatcg tagttcttgc tatcgagctt aaactcgttg cgaagcaaaa agttaaagtt
    28381 acgctccttt aaccgggttg attgcagatg aaggttgtaa accctaatgg tatcggttcc
    28441 cttaactata tcggcataca tacatgcgtt aaacgaattg ggaaacgata tttcgcccac
    28501 actcacaaca ggttgattgg ttaaaatggc caagccgtaa gccgacgatg gccgcctaag
    28561 tatgaacgag gagtaaacct tcatccctga ctgctgctca acatcaagat ggctcagctt
    28621 gccctcccta agataaaact cctgcaggca aaccacatca aagtcgttgt ccttaatatg
    28681 agttaggata tcggaatatg agggtttcag ctttgaccac gagtaaagcc taaaaaggtt
    28741 tacattgtaa gtcatcaccc ttaaatcagg attggaaccc tttgcagcac tcccatacgg
    28801 aaagcgaata aagccattga aatggctaac gccaagcaga atggctgcaa gtgaaatgaa
    28861 aactaacttt tgccaacgaa aaatccagta aaaaagaaaa agtaggttta ctatcagaaa
    28921 gaaagggtaa agcaatccgt atagcgcaac aaaccaaaaa tcttcagggt ttatgtaaac
    28981 gctcacatag gtaagcacca gcaagagggc aaaaagtaca tttaccccca ccaaaatccg
    29041 atggattagc cttacaatga gcatcaatta atgttcctgt tattggcctt aaacagaatg
    29101 tccttttcct ccttggttaa gctatcgtag ccggagcggg caatcttatc gaggatatca
    29161 tctatctgct tttgcttttg tgctttacgg tagttgtagt cgtaatcggc attatggctg
    29221 cggtaagtta ccttcattcg gttccgtttt ggaaacctaa tgctgctaat gctgtaaaat
    29281 ggcttagcca aatcggttcc gctacgcaga gcagcaacgt agagaaagcc aaacagggct
    29341 cccccaaggt gcgcaatatg tcctccagcg ttggaaacag ggatgctaat gatatcgatg
    29401 acaacggtgg caagggcaag gtactttagc cttactgcac caagaaaaag caggtaaaca
    29461 gtaaagttgg gctgaagtgt ggccactgca ataaccacgg ccagcactga agccgatgcg
    29521 cctaaggcta acgatacctc agcatagcct ttaaatggag gtaacaggtt gaacgctaaa
    29581 atgtatagta aagccccact gataccgcca agcaggtaaa cccatgtgag ctgcctctgg
    29641 gcaaagaatt gcagaaacag ctggccaaac cagtaaagcc atagcaggtt aaagagaata
    29701 tgcaaaaaat cctcatggta aaacatgtag gttacaatag tccatgggcg aaccatgagc
    29761 gattgaaggt tggccggcac tgccataaaa cctgttatcc agcccgaaga acttgcagac
    29821 aggaaaagga aaacccttag caaaccaaaa agtaaaaata caccaaggtt gatatagata
    29881 agccgggtaa ggttgctacc cagccgaaac gaactcttaa tctcatccca aatagtcata
    29941 acacaaaaat gttaaaccgt tcaaaaaacg aactttaaag gtattagtaa agttaaccga
    30001 ttttcggcta aatatcaaca ctaataaaaa acatcggtgc gcctttgcca aagcttnnnn
    30061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    30121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnatta caataatctt tttaccatca
    30181 ggggtaaggt ttggttcatg gcatgcaaaa agcttatcaa ctatctcctg catttcaaac
    30241 tgactcaact gtttaccgta tccaatagcc gatgctttgg ccatggtaag cgcaatgcga
    30301 tgtttagcat cgtcgaatgg gagggcttgg ttctcgtgca taatggcaag caggttttca
    30361 accagctttg cgggatcggt aaccgatgag ctaaccggca tgctattcac gcttattgtg
    30421 ttgtggttta ggttgctgat ttccataccc aaacgcgata gctcatcggc cttgctcatc
    30481 agcagctcaa agtcggcagg tagcagctcg atggcctgtg gaaaaagctc gcgctgtttc
    30541 aggtttatat cggcttgcat gccgcttata tactgctcgt aaagtatgcg ctcatgagcc
    30601 cgtttttgat caatcaccat cagccccgac tttacggtgg taagtatgta cttgcccttg
    30661 aactggtaaa aacggttggt gttttcggga gctttactca gcatcacttc cgattcaaag
    30721 gtttcgattc gggtttgaat gggggttgga tcctggggta aatcggtaaa aagttgctcc
    30781 cagccggttg ccgattcacg caatttatcg gcgctatgag gcttacgaac attcctgtta
    30841 tgctcatcga aagggttgta ggtggggtca acgtcaatgg tgggaaaagt taccggtgca
    30901 ttcttgggaa ggtatgggat atcgaatcct ggagcagaat caaaatcgat tgggggcacc
    30961 acagagaatt ttccgagcga ttcccgtacg gcagcgtgta atatctgcca gatcattcgc
    31021 tcgtcctcaa actttatttc ggtttttgta gggtgtatgt taacatcaat ggactgcgga
    31081 tcgatatcaa agtaaatgaa atatgagggg taggtatcgg gtggcagtaa gcggttatat
    31141 gcgttcatca cagccttgtg caggaaaggg ttcttcatga aacggccatt cacaaaaaag
    31201 aactgttcgc ctgcagtttt cttagcccct tcgggcttac ctatataacc accaatatta
    31261 actattgatg tttgcgtgga gcaatccaca aggtgttggt ttatggtctt accaaatagg
    31321 ccaataactc tttgcttcaa tcccgatgct ggcagcctgt agagttctga accattgtgg
    31381 ctcaaactaa aactaacttc ggggtaagcc aatgcgaccc tctgaaactc ggttataata
    31441 tgcttaagct ccacggaatc gcttttaagg aacttgcgcc ttgccggcac attgaaaaag
    31501 agatttttaa ccgcaatttg ggtgccggta gaacaggcca ccggttgctg gctaaccagt
    31561 tccgaagccg aaatgttaac ctccgttcca acctcatcaa cagcccggcg ggttcgcagc
    31621 tcaacctcgg caacagcagc aatggatgcc agtgcctcgc ccctaaaacc catggttcgg
    31681 atgttaaaga ggtcggctgc ctcgcgaatc ttggaggtgg catggcgctc aaagcaaagc
    31741 cttgcgtcgg tttcgctcat tcccacccca tcgtcaatca cctgaattaa acctttacca
    31801 ccctccctga caattaggtt gatattacga gccccagcat cgatggagtt ttccaggagt
    31861 tccttaacca ccgaggccgg acgctgaacc acctcgcccg cagcaatctg attggcaatg
    31921 gaatcgggta acagttgaat tacatcggcc atactagcgg aaaaatacga agtaactgat
    31981 tagcagaaga attgcaataa taatcaataa cctgatattc gactgcctac tcgaaggtct
    32041 gctaattttt cgtgcacgtt gaaactgtcc cctaatgctt ggcacaaaag gtttatcctt
    32101 atcggtgagc cccaattcct ttcgaactcg ttcctcgcgc tcacgcatct cctccttttg
    32161 ctcattgtag tatataggct tatacctaaa acccttgggt tttggtatac taaaaaactt
    32221 gaatcctgcc atgacttgca tctcctttta gaattacaaa gttagccatt tatcattatg
    32281 cttcaatttt ttatcaacac cacacttgac catttaatca aatat
//
LOCUS       DAOSAL010000023        31864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_1038_length_31864_cov_51.907668, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000023 DAOSAL010000000
VERSION     DAOSAL010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 31864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31864
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1038_length_31864_cov_51.907668"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            504..686
                     /locus_tag="PK049_04945"
     CDS             504..686
                     /locus_tag="PK049_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60936.1"
                     /translation="MGTPWVELPRDTIRHACGVTHVVSLTKPTATFHPRPIAGSGFYA
                     SACFFRFIPSQGKELG"
     gene            1116..1556
                     /locus_tag="PK049_04950"
     CDS             1116..1556
                     /locus_tag="PK049_04950"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOK60937.1"
                     /translation="MTLVSFYNPLASRRTGVDFERVFESFVKSASEMANARLSYPAVN
                     IYEEPERFRLEVAAPGYQKDDFKVSIDNDMLKISAEKENNPVEGEAYTRCEFLTGSFE
                     RTFVVGKAIDTNKIDARYENGILTVFLSKRDEAKPQKPRSIKID"
     gene            1738..3735
                     /locus_tag="PK049_04955"
     CDS             1738..3735
                     /locus_tag="PK049_04955"
                     /inference="COORDINATES: protein motif:HMM:NF015926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HOK60938.1"
                     /translation="MNTNELNDPKDQNLENIEGTIVSSNAGNHDVADSVNDLVDVNAD
                     KPMSISDEAYDEEPNDEELKPLEMDDEHVDFDEEDEEEAEIDEEDDEPHVDYSNLSKD
                     ELVQTLKDLINNRPVQKIRQKVESLKSIFYRKHKAEVEKLRKEFVDAGGDLEQFSLPE
                     DNAEMQFKELLKQYRAKRAQYNNQLEEQKQHNLEQKQQIIEKIKDLVNSTEPVGKIYQ
                     EFRDLQKQFLEVGPVPQASLNDLWNNYHHHVQAFYDFVKISKELRDLDFKKNLEAKIK
                     LCEQAEELLLEPSVVVAFKKLQKLHEQWREVGPVPRENQNEIWERFKEATTKINKKHQ
                     EHFESIKEQQKKNLEAKAELCEKAEELAAQNPKSVKEWNKLSKELIELQKLWKTIGFA
                     TKKENNKVFERFRSACDTFFNKKRDFFKDIRDVQVNNLQLKTELCLQAEALKESTDWK
                     KTTEDLINLQKRWKQIGPVPRKNSDEIWKRFRAACDYFFEQKSKHFASVDNSYEENLK
                     AKEALIAEVEAFEFGEDMEQNLNALKEFQRRWTEIGFVPIKVKDDVQKRFRAAIKKHF
                     DKLNAQEGKNKVVRFQAKLEEAKANPRAMSKLRQDRERMFNRMRQLENDLTVWENNIG
                     FFAKTKNAEAMIEEVKRKIERAKEEIAELSEKIKLIDKLEQ"
     gene            3747..5363
                     /locus_tag="PK049_04960"
     CDS             3747..5363
                     /locus_tag="PK049_04960"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOK60939.1"
                     /translation="MPQSSSTKYIFVTGGVASSLGKGIISASLGKLLQSRGYTVTIQK
                     LDPYINVDPGTLNPYEHGECYVTEDGAETDLDLGHYERFISLPTSQLNNVTTGKIYQT
                     VISKERRGDYLGKTVQVIPHITDEIKRRIKLLGTKNKLDVVITEIGGTVGDIESLPYI
                     EAVRQLKWELGPNNSAFIHLTLVPFLSASGELKTKPTQHSVKELLENGIQPDVLVLRT
                     EKHLSDDIRRKVALFCNVEPEAVVESIDVKTIYEVPLLMLKEKLDLTILRKLNLPASE
                     PKLDDWINFVNRVKNPSHRVKIGLVGKYVQLPDAYKSIIESFIHAGAANDCKVELELI
                     QSEDINASNLSEKLGHLKGILVAPGFGHRGIEGKILAVKYARENKVPFLGICLGMQCA
                     VIEFARNVLGLTDANSTEMVRTTPYPVIDLMEDQKGITDMGGTMRLGAYPCVVAKGSN
                     THKAYKKLEISERHRHRYEFNNKYFEQFQNAGMIPVGINPDTNLVEIMELSDHPWFVG
                     VQFHPEYSSTVLKPHPLFVDFVKAAINFNK"
     gene            5441..7294
                     /gene="yidC"
                     /locus_tag="PK049_04965"
     CDS             5441..7294
                     /gene="yidC"
                     /locus_tag="PK049_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOK60940.1"
                     /translation="MDRNTVIGLVLIFLIMIGFGYLTQPSKEEREAYQRKADSIARVQ
                     EEEHQKRLAEEQAKALLSDSAKKQEAITQFGLFSDAASGSNKFITLENDLMRVTLTAK
                     GGRIYSVELKKYKTYSGEPLVLFTGDENTFGLQFWGDNVAVKTNDLFFTPQTTDSIIT
                     ATTDSVSVTMRLAAGGNSYIDYIYTIKPGSYMLGFDIKFTGLEKNIGNRLGYIDLIWD
                     SKLLRLEKGLENERNYTTIAYQFFTGDYEEFSSRSKEDSKELNNKIKWVDFKHQFFSS
                     IIVANDHFVNADLKAVNIDDGKHVKQFSARLALPFQNKESESIGLKFFFGPNHYKTLK
                     SYNSGFEKVVPLGRNIIRWINKYIVINVFNFLSRYISSYGLIILLLTIFIKIIISPLT
                     YKSYLSSAKMRVLKPQVDEINAKYPKQEDALKKQQAVMALYKKVGVNPMGGCIPILIQ
                     FPILIAMFRFFPASFELRQQSFLWADDLSSYDSILHLPFSIPWYGDHVSLFTLLMAVS
                     LFITSKMNTDQMGDTNAQMPGMKFMMTWMMPIMLLLWFNNYSAGLSYYYLLSNVITIG
                     QTYLFRRFVDDKAILAKLHENAKKPVTKSKWQQRLEDMAKQQEQLRKKKGK"
     gene            7971..9227
                     /gene="hutI"
                     /locus_tag="PK049_04970"
     CDS             7971..9227
                     /gene="hutI"
                     /locus_tag="PK049_04970"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HOK60941.1"
                     /translation="MQILILNIKKLVQVEDKPAVWKAGNDMAKLKTIDNAYLLIDKGL
                     IADFGPMSKAPDISSLSGDVLIYNAFGRMVFPSFCDSHTHLVYAGSREIEYIDKIKGL
                     SYEEIAKRGGGILNSAKRLAETSENELYNQSLARIYEIIRKGTGAVEIKSGYGLSTES
                     ELKMLRVIKRLKETTPITIKSTFLGAHAVPAEYKGRQSEYVDLIINEMIPVVASEGLA
                     DYIDVFCDKGFFTVEDTERILMAGIKYGLRPKLHANELDFSGGIQVGVKYNALSVDHL
                     EFTGDDEINALLGSETMPTLLPGAAFFLGMVDPPVRRMINAGLPIALASDFNPGSSPS
                     GDMKFIMSLGCIKLRMLPEEVINATTINGAYAMGISDTHGSICKGKVANVFITKPIPS
                     YEYMPYAYTSDLIETVILNGSIINDK"
     gene            9235..9954
                     /locus_tag="PK049_04975"
     CDS             9235..9954
                     /locus_tag="PK049_04975"
                     /inference="COORDINATES: protein motif:HMM:NF025221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 family protein"
                     /protein_id="HOK60942.1"
                     /translation="MKKNLLLPIAILISACAEISIPTNFPIPQGSGSKPLTNAEVVQG
                     LKEALAVGAKNSTGLASQVDGFYKNPKIFIPFPPEAEKVRKTLVDAGFSKQVDEFTLT
                     LNRAAEEAAKKALPIFTDAITKMSFTDAMGILRGPNNAATEYFRKNTSEQLFAAFKPQ
                     VHEAIQKVKLTSYWEPLATTYNRLTLLTGGKAVNPNLDDYVTQKAIDGLFVLIEQEEL
                     KIRKDPAARVTDILKRVFGNN"
     gene            10107..11801
                     /gene="ftcD"
                     /locus_tag="PK049_04980"
     CDS             10107..11801
                     /gene="ftcD"
                     /locus_tag="PK049_04980"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006254408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HOK60943.1"
                     /translation="MSLKKLIECVPNFSEGRDMTIIKQITDEIEAVEGVKLIDVDPGK
                     ATNRTVVTFVGTPDEVVEAAFRATRKAMELIDMSKHHGAHPRFGATDVCPLVPVANIT
                     MDEVVEYARKLAKRIGEELGIPVYCYEYAAFEEKRRNLANCRAGEYEGLPKKLQDPAW
                     KPDFGPTQFLPKTGAIAVGARNFLVAYNVNLNTTSTRRANAVAFDIREKGRPMRVGNP
                     VTGKIATDENGNEIWVPGSLKACKAIGWFIDEYGIAQVSINLTDITVTPIHVAFEEAS
                     KKAAERGLRVTGSEIVGVVPLSAMLEAGKYFLRKQQRSLGIPDREIIKIAVKSMGLDE
                     LYPFDPDKKIIEYILEEKTGKKLVDRTLEGFTQETASESPAPGGGSISAAMGAFGAAL
                     ATMVANLSSHKPGWDDRWEEFSNWAEKGKFYMEQLIKMVDEDTAAFNRIMEAFGLPKG
                     TDEEKAARTKAIQDATLYAIEVPFRVMNLCFESMEVIKAMAEHGNPNSVSDAGVGALA
                     ARSAVMGAFLNVKINASGLKDKDKANEFISRGAEIVKKAQQMEAEILSIVESKIMA"
     gene            complement(11868..>12683)
                     /locus_tag="PK049_04985"
     CDS             complement(11868..>12683)
                     /locus_tag="PK049_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60944.1"
                     /translation="NISNKLYLGASLGITDFNYTFNSTYSEDAKESNPSRPNGDRFYY
                     MDYKQYVETKGSGFNLKIGGIYTPIPEVRIGLSFHTPTFYSFDDAFSSSIITNFDTTG
                     TEVIFQSNSPLGRYDYHFETPMKITGSVAIIIMQKGLVSIDIEHLDYSTMRFRRGGDG
                     DTFWDLNSQMGNTFKSVTNLRFGGEYKIGDIALRGGYAYYPSPYKSGFLNDKSKIQQI
                     SGGIGYRTGNVSIDFAYLRTLQDVKYVFYDLNIPSVTNKVRDSRVLLTFGFRF"
     assembly_gap    12684..12783
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12784..13293)
                     /locus_tag="PK049_04990"
     CDS             complement(<12784..13293)
                     /locus_tag="PK049_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60945.1"
                     /translation="MKTYRSLTIGFLATLSTLGYNLNAQNLADGLRFSQTHNNGTARF
                     VSMGGAFGALGADFSSIAVNPAGVGVYKSGEFTITPSFKTQKVSSTFLGVSASDNKNR
                     INLDNLGFVMSFKPYKSEDKGILAYNLSFGYNRTNDYHSNAITLGDYNNYSIMDYFSA
                     ITNGKYTPSQ"
     gene            complement(13373..14773)
                     /locus_tag="PK049_04995"
     CDS             complement(13373..14773)
                     /locus_tag="PK049_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60946.1"
                     /translation="MKTKLFNPALTLAVFMLASCSTGLQVSKTSSWDDEIYGTTNSKS
                     TKVQVANVSPVAPSAKSEQYNKLDGKFAEALKALEDSSKNDTVIYKAEDPNPYRRILS
                     DSYQESYERRLRGFEDPRYGIEDWSVYYSDDWRYAQAYDPSYYRVVVMGSNVWVEPWY
                     IYNSFGWPRSHFYFGFGFGWGYNPWNSYYLGYYWDPWYYNPWYYNPWYYSPYVASNAY
                     WSGWYDATYYWDKYTPNSNRYYYGRRGNTTNNTDQYVGGRSTSVIPGDGQIVTSRRTR
                     TDVPAETNPVVTTRETQTRVTPTYETRRGEGRTTSTGNQTVVRRDGTSTRVSDGTGDF
                     SSSRTSRPTREIGISEPTRRGTTPTYERPRTGNSGEYNSTRRVENPSTRTTITRNPST
                     TTPTRTTTPTYNRPNRVSTPSSSQPSTRSYDYERPRSSSSGSSSSNSSVSRSSSSSSS
                     SSSSSSSSGTSTTRRR"
     gene            14880..16355
                     /gene="proS"
                     /locus_tag="PK049_05000"
     CDS             14880..16355
                     /gene="proS"
                     /locus_tag="PK049_05000"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOK60947.1"
                     /translation="MAKEITSRSENYSQWYNDIVVKAGLADYSAVRGCMVIKPYGYAI
                     WEKMRDQLDRMFKETGHQNAYFPLFIPKSFFSREAAHVEGFAKECAVVTHYRLKNDPN
                     GKGVVVDDEAKLEEELVVRPTSETIIWNTYKTWIQSYRDLPILINQWANVVRWEMRTR
                     LFLRTAEFLWQEGHTAHATMQEAVEEAERMINVYATFAREWMAMPVVVGKKSESERFA
                     GAVDTYAIEALMQDGKALQAGTSHFLGQNFAKAFDVKFLNKEGQQEYVWATSWGVSTR
                     LMGALVMTHSDDNGLVLPPKLAPTQVVFVPIYKSDEELSAISEVVHKLRSQMHSKGIT
                     TYYDDRDNYKPGFKFAEHEMRGVPVRVAIGPKDIEKGTAEVARRDTLQKQYVPQEQLA
                     DTIVALLDDIQQNLYNRALAFRENNTVKVDSYDEFKKIVAEKGGFVLAHWDGTPETEA
                     QIKEETKATIRCIPLDMPEEEGKCILTGKPSKRRAIFAQAY"
     gene            16466..17167
                     /locus_tag="PK049_05005"
     CDS             16466..17167
                     /locus_tag="PK049_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009599067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60948.1"
                     /translation="MENQNTQQKIQNLLTEKAVVKQKIYDNTLEVFGLIKEVLKEIEE
                     DFNDKLRGYDKRVKLEYNDRGKYEAELKVAGDLLIFSMHTNVFEFDRDHSIWKLSYVT
                     DNVLNSYCGIINIYNFLADSFKYNRLEDLGYLIGRIFVNREYHYFVEGKRQMGFLYNN
                     FGSAVIDKKSLRTIIETAIMYALEFDLLVPPYDVVKIASVVQLNTKIENAKLQTGKRL
                     GFRFNSDDVLEEKEQ"
     gene            17189..18817
                     /locus_tag="PK049_05010"
     CDS             17189..18817
                     /locus_tag="PK049_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HOK60949.1"
                     /translation="MKGKTLLLAAVATLTVIGCNTKKTQETEMQKKVNEFAPVELRVD
                     IISLSDKEKQMLPLLFEIAEIMDDLFWLQTYGDKEELLSKISDEATRDFVMINYGPWE
                     RLNDNKPFVEGYGEKPLGANFYPKDMTREEFEAFQAPDKTSLYTIIIRNTDGKLESVP
                     YHVAYKEKIDKAADLLRKAAELAEDEGLKKYLTLRADALQTDDYFASDLAWMDMKTNN
                     IDFVVGPIENYEDALFNYKAAYEAYILIKDLDWTQKINRFAALLPKLQESLPVEPEYK
                     KEKPGIDSDLGVYEVAYYAGDCNSGSKTIAINLPNDPRVHLEKGSRKLQLKNAMKAKF
                     EKILLPIGNMLIAEEQRDNIKFDAFFENVMFHEVAHGLGIKNLVNGKGTVREALKEMH
                     SALEEGKADILGLYMVSQLAQMGELPEKDMMDNYVTFVAGIFRSVRFGAASSHGKANM
                     VCFNYLKERGAFERIPETGTYRVNYEKMTEAMNSLAKDILTLQGNGDYAAVKKLFEEK
                     GFITDELQADLNRVAEANIPRDINCIQGKELLGL"
     gene            18897..19469
                     /locus_tag="PK049_05015"
     CDS             18897..19469
                     /locus_tag="PK049_05015"
                     /inference="COORDINATES: protein motif:HMM:NF038262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HOK60950.1"
                     /translation="MNLSFNFIKSVSNIYDEMIDNGFSLVYLGEFSHEITKMFTSMAE
                     SDMEKNSEERSVQRKVYHVMVETLQNMNKHSDEIQERNIGKGLFIIGKKHDTYYIITS
                     NKVARKHKDHLENALITVNSATPEELKEMYKRQIREGNLSDKGGAGLGLIDIARKTGE
                     PLNYQFLQLDDQNFFFILKVEINAKKFAKE"
     gene            19486..21324
                     /locus_tag="PK049_05020"
     CDS             19486..21324
                     /locus_tag="PK049_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60951.1"
                     /translation="MTDFKLLSYTRGKTIIALVMMVYFSFFGFINYSSFAQSDSNRVS
                     SDSIKKKEDKISQTVYKWFGRQNVNVAPLTNEETVNRFLKHEGKVISQISIIRFDAFG
                     YSVYDTTIKPERNIEKFGNSLHLKTWKKIIQNNLLFKEGDKVVPLDLAETEQLLRQSN
                     FFEDANILVQPLSDSSHVHVYVITKDIWTISAGVQYSSPEKMKLALSERNFAGLGIRA
                     KAIAYYNKTFTDKWSYQGEFDVPNYFGSYISSSFFVRKGHSYETYLMRFNRDFFASKT
                     KYAGGIELLKSDEPYRVFTDDSVVQISYQSQDWWIGRSVRVSRRSGTSAPLNLVFALR
                     YYRRDYFQSPPVSIYLNPIFHSAENYVVSLGLSNQNIYRSSLYFGFGNTEDIPVGFKI
                     QATSGIQQSTFQRRFLIGGELSAAEVTPWGYLFLSSRLGGYLAEGPKVEQAVINLRAQ
                     YFSNLYHIGRSDIRHFIRYDFTRGAARFSGEREYVVLTNNYGVRGLRSPDLYGQTRLM
                     FNFESMLYSPHYIYGFRPAFFVFADFGMVGESDELIYQNTLYSGFGVGLRLKNESLIF
                     PTFLFRIGYYPKLPPDAEVAYWFISTENRRNFEQFRIKEPYILQYQ"
     gene            21388..22410
                     /locus_tag="PK049_05025"
     CDS             21388..22410
                     /locus_tag="PK049_05025"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde reductase"
                     /protein_id="HOK60952.1"
                     /translation="MTKTILVTGGTGYIGSWVVKELLEKGYRVRVPVRSFNRKDKYLH
                     LQSLPNSTNLLELFEADLLIDGAFDEAAEGTDAIIHMASPFTLRFKDPQRDLVDPAVK
                     GTRNVLGAASKSSTVKRVVLTSSVAAVHGDNIDMEEQGLTEFTEENYNTTSSLKHQPY
                     SFSKVQAEREAWRINAEQSKWELVVINPALVLGPSLAGNSNSESLQIMKDLLSGKYRF
                     GAPDLYFGFVDVRDVAKAHVLALENPSVSGRFIIAERVANFMELVAIINNKFPGQFKL
                     PKMVAPKWLLYFVSPLFGLSPRFVARNVGYRIAFSTSRSRQELGLGYTPFEHTVVDMV
                     NQFKKN"
     gene            22457..23779
                     /locus_tag="PK049_05030"
     CDS             22457..23779
                     /locus_tag="PK049_05030"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HOK60953.1"
                     /translation="MRSKYIHRVSNFDRFAKNLAYEVENTDVGILLRGSDRFTIPETN
                     FTSRFQLAAALGISSACSPNSNHFDELDEFLSAHNDWVFGYFTYDLKNQLEHLQSNNP
                     DGVGLPDMFFFVPRILILVNGEIAEFHYLQQEVSQEEVSDLIDRLEGDLEELDHNVSV
                     VFRSHYSYNDYLNTVSAVKQHIARGDVYELNLCQEFFANNVRITPYVVFNKLYDRSPT
                     PFGAFLRVNDKFLMCASPERYLRRDGDWLFSQPIKGTAPRMESDESDQQEIFRLKTDP
                     KERAENVMIVDLVRNDLSRIAKRGSVRVNELCGIYTFPQVHQMISTVSCELADGLRPT
                     DPFRATFPMGSMTGAPKVRVMELIENFERSKRGLYSGAVGYFMPNGNFDFNVVIRSLL
                     YNRSSGYLSFTVGGAITFKSQPEAEYRECLVKAKAILETLNAQIDAAK"
     gene            23766..25094
                     /gene="tilS"
                     /locus_tag="PK049_05035"
     CDS             23766..25094
                     /gene="tilS"
                     /locus_tag="PK049_05035"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOK60954.1"
                     /translation="MLQSKMIDFVREHQMANDQDKILVGVSGGIDSMVLLHLLVHAGY
                     SVGVAHCNFSLRGDESDADEHFVRYHCLMNGLPVHTIKFDTLEYAKENGLSIQVAARE
                     LRYAYFDEICKEHKYTRIAIAHNLNDSVETVLLNLSRGTGLRGLTGIKPVNGNIIRPL
                     LFATRVQVQEYATLHNISYREDSSNASDKYKRNYIRHNVLPLLRELNPSVDISIYQTA
                     QHLREAQLIVDSFLTQLRKEIVNENGGVFYIDIEKLKNQAAVNIFLVDFLSDFNFTPA
                     LTTEVCELLESSIGKRVESDTHVIFRDRNHLILQQKQKRQSVECQIDVNITQIDQPVK
                     LSLSKLDYSQGFVFGKDTKYAYLDLEKLKFPLTIRPWQPGDRFMPFGMTSFKKISDFL
                     VDIKIPLPDKEKVFVLTSENQIVWVVGYRIDNRFRVDENTQKILLIKFDE"
     gene            complement(25103..26980)
                     /locus_tag="PK049_05040"
     CDS             complement(25103..26980)
                     /locus_tag="PK049_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011107811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOK60955.1"
                     /translation="MALIYLQAENVSKSFGDLEVLSDVSLSLFQNQRTALVARNGTGK
                     STLLNILAGYDTPDSGKVTLRNDISVGYLPQEPQLNLSKSVLENVFNADDAISVAIRN
                     YEKAIESTDKMALQNAIDEMDRLNIWDREQKAKQILSVFQLTDINQPVQLLSGGQRKR
                     VALAAVLISNPDLLILDEPTNHLDLEMVEWLEDYLTRSQKTVLMVTHDRYFLDRVCTD
                     IIEIDQKQVYTYNGNYSYFIEKRDERIRAKLAEIDRASNLLRRETEWMRRMPQARGTK
                     AKYRIDAFYKLRETALQKIHSNDVEIGVSASRLGKKIMEVHNLSKSFDSKVILKDFSY
                     TFNRFERLGIVGANGTGKTTFLNLITGTLPPDSGTIDPGATLKIAYYRQSGMNIPEDV
                     KVIDVVREIAEVITLANGKTITASQFLNNFLFTPDRQHTLVRKLSGGEKRRLYLLTVL
                     MQNPNFLILDEPTNDLDIFTLNVLEDYLINFPGVVVVVSHDRYFMDKLVDGLLIFEGN
                     GEISGFPGNYTEYREWAKEQEKSNAEQQTSSKGKTDSKRPQPERKKSKLTFAQKKELE
                     KLTDEIVKLEKEKKEIEGLLNGGIQDAKLLMDISNRFSEISKLLEDKEMRWLELNEFE
                     E"
     gene            27317..27976
                     /gene="nth"
                     /locus_tag="PK049_05045"
     CDS             27317..27976
                     /gene="nth"
                     /locus_tag="PK049_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005468908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOK60956.1"
                     /translation="MMNSKKELYRFVIEYFEKTRPIAETELHYSNPYELLVAVILSAQ
                     CTDKRVNLITPNFFKSFPDAVSLANASVEDIYKIIKSCSYPNSKAKNLLGMAKVLMTE
                     FNGEVPSDINLLQRLPGVGRKTANVIASVVFDKPAMAVDTHVFRVANRIGLVSNAKTP
                     LAVERQLMENFPLELIPKAHHWLILHGRYVCTARKPKCTECGLTLVCKEYKKISKKAE
                     K"
     gene            complement(28020..28652)
                     /locus_tag="PK049_05050"
     CDS             complement(28020..28652)
                     /locus_tag="PK049_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60957.1"
                     /translation="MKRVVISSLLLFATTGLFAQIFVGGSVGFNTTGGKIENGNTSVD
                     KVTQTSFSLAPKAGMFLSEKLAVGAILGFNLQSEKTPGTPEEIDRTTTFGLTPFARYY
                     AFSLNKFSVFAQGNLGFSYGVEKHKEGSTTTTGPKTTTIGISAFPGISYKLTDKVELE
                     AVIGGLNLNFNRVSVKNNNTTNITNTFGVGANLDAIATTGFITIGAIVKL"
     gene            complement(28745..29992)
                     /locus_tag="PK049_05055"
     CDS             complement(28745..29992)
                     /locus_tag="PK049_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60958.1"
                     /translation="MENSIRNNLDNPEVLEQLYRKNRKGFEKAFFSIYSEISSIPLAK
                     FWFTRLTADNKILFNVNLKEIAYVLISCLIVSIVMLIPWLFGFNPHENNFYERNLALT
                     ALLGVTLYTAILKNINLKKTAAIVGFFIISAIYVNVLPKTESSSTTLIYIHLPILLWF
                     IFGIVFSEFELKNNEKRIEFIKYNGDLAVLFSIFAIAGMILTAFTIGLFSAIGINVEK
                     IYFELIVIPGVVSVPIICTYLINKFEYLSNKIVTIVANIFSPLILITLIIYLISIVAK
                     GSNPFVNRDFLIIFNAMLIGVVALVIFSISERVNNSTNNYYTIIFTALSTIALLINVV
                     ALLAILYRITEYGITPNRLAVIGSNLLFFTHLILILTNLLRVIRKKQHLTIIEQVTAK
                     FLPYYGIWLIFVVFILPFIFNFK"
     gene            complement(30288..30361)
                     /locus_tag="PK049_05060"
     tRNA            complement(30288..30361)
                     /locus_tag="PK049_05060"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30325..30327),aa:Arg,seq:ccg)
     gene            30452..31597
                     /locus_tag="PK049_05065"
     CDS             30452..31597
                     /locus_tag="PK049_05065"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOK60959.1"
                     /translation="MKANFVILSSLILLSFRLSAQVVFVVDSFPARTRPDDTIFMASE
                     LNGWNPHDISWAFKRTTNRYELKLTHVKDTFEYKLSRGSWQLVEVDSNGGDIRNRIYF
                     PPTDTVSIKVNGWRDMFEAAQRKSTASTNVRFLPSTLEMKQLNRRRAVRLYLPPNYSK
                     RQQFPVIYMHDGQNVFDEATSFAGEWRVDEIMDSLYYSCGFAAIVVAIYNDSKERINE
                     YSPWRNDSLGMGGDGDKYAYFVAKELKPFIDKNYRTNPEPKFNAVIGSSMGGLISLYI
                     GLKYPDAFGRVGVFSPSLWFSPKVFEYVSKYKRRADQKIYLLAGEKESETLVEDMKML
                     EHHLYKAGYKDEDYVKMKIAPDGRHSEWFWSREFTEALEYLFEIRKN"
BASE COUNT         9254 a         6265 c         6960 g         9285 t
ORIGIN      
        1 ggtgtttggt gtttggtgtt tttgccatca acaatcaacc atcaaccatc aactgtcaac
       61 catcaactgc caactatcaa ccatcaactg tcagctccgc ttgtcatcct gagcggcctg
      121 tcccgcccta gcggggagcg aaggatctct aatcggccct aaagagatgt aaacatgaca
      181 atagtcttgg gaactgatca aaaacaagta acacagagtt acacaatgta atacagtgtt
      241 tcacagagtg aatgacatga cattcacgga caacgaacaa cgatgcacga acaacgaata
      301 acgtcttcag ccccgtaggg gcgtaatatt tgtaacacca tggaactccc gtatgtaaag
      361 cgatgcacgc tatgatattc caaagagcga aattgaatcg ttgcatcgca aaggaaaaac
      421 attgcaaaga gtgattttca tgttctttct tgtcttgata caagaaagaa ccaaagaaaa
      481 tcaaggctga aaagcccgac ccgatgggta ccccgtgggt ggaactgcca cgcgatacta
      541 ttcgccacgc ctgtggcgtg actcatgtgg tatcgcttac taagcccacc gccacgttcc
      601 acccccgacc cattgccggg tcaggctttt atgcctctgc ttgcttcttc cgcttcattc
      661 cctctcaggg aaaagagctg gggtgaggtc gtatcccacg aataacgaac aacttacacg
      721 aacaacttca tttgtcatcc tgagcggcct gtcccgccct agcggggagc gaaggatcta
      781 tattcatcca tcgttgagat gtttcagcgg agtctttccc tattatatcg ggactcaaaa
      841 tgacaaacaa ccttaactaa acgacgttgt atgggattgg ttactctgaa ttctgcatgc
      901 attgtttact ttaaaatatc aacccacaag taaatcgcat gctattaaaa tgcgcatgtt
      961 attaaaagta ttcatgattt cccgaggtgt tttagtgtca tgttgacata gtaattttgt
     1021 atttatatga cataatgact tgtttttctg tatggcatac tattagctaa atattaaatg
     1081 tgaatgtgaa tatcaacttt aaaaattata atactatgac acttgtaagt ttctacaacc
     1141 cgcttgcctc aaggaggaca ggggtggatt ttgagagggt gttcgaaagc tttgttaaat
     1201 cggccagcga aatggctaat gccaggctca gctatccagc cgtaaatatc tatgaggagc
     1261 cagagcgctt tcgtttggag gttgctgctc ccggatacca aaaggacgat tttaaggtaa
     1321 gtatcgataa tgatatgctt aagattagcg ccgagaagga aaataatcct gttgaaggtg
     1381 aggcttatac ccgctgcgag ttcctaacag gctcatttga gcgaaccttt gttgttggaa
     1441 aggctattga taccaataag attgatgccc gttacgagaa tggcatcctc acggttttcc
     1501 tttccaagcg cgatgaggct aagccacaaa aacccagaag cattaaaatt gactaagtat
     1561 ttatattgca aatattattc agcccgaatc cctttaaaat cgggcttttt ttgttttgcc
     1621 agtcaattat tctttattct tttacgttaa tttgtttgcg ttacgcccga ttatatgtaa
     1681 tttcgtaggc aaaatttttt aaatactgct gatttataac cggttaagtt ttgaactatg
     1741 aatactaatg agctgaacga tcctaaggat caaaacctcg aaaacatcga gggtacgatt
     1801 gtatcttcca atgccggcaa ccacgatgtt gctgatagcg tgaacgattt ggtcgatgtt
     1861 aatgctgaca aacctatgtc aatttccgat gaggcttacg atgaggagcc caacgatgag
     1921 gagctgaaac cccttgaaat ggacgacgaa catgtcgatt ttgatgagga ggatgaggaa
     1981 gaggccgaga ttgacgagga agacgatgag ccccatgtgg actactcaaa cctttcaaag
     2041 gatgagctgg ttcaaaccct gaaggattta atcaataacc gacccgttca aaaaatccgt
     2101 cagaaagttg agagtttaaa gagcatattc taccgcaagc ataaggctga ggttgaaaag
     2161 ttaagaaaag aatttgttga cgccggaggc gaccttgagc agttctcgtt gcccgaggat
     2221 aatgctgaaa tgcagttcaa ggaactgctt aagcagtatc gtgccaaacg cgctcagtat
     2281 aacaatcagc tcgaggagca aaaacagcat aacctggagc aaaaacagca aattatagag
     2341 aaaataaagg atctggttaa cagcaccgag cctgttggta aaatctatca ggagtttagg
     2401 gatttacaaa agcagttcct tgaagttggc cctgttcccc aagcaagctt aaacgatttg
     2461 tggaacaact accaccatca cgttcaggcc ttctacgact ttgtaaagat tagcaaggaa
     2521 ctccgcgacc tcgatttcaa gaaaaatctt gaagccaaaa tcaaactctg tgagcaggct
     2581 gaggagttac ttcttgagcc ctcggttgtt gttgcattta agaaattgca gaaactccat
     2641 gagcagtggc gcgaggttgg gccagttcca cgcgaaaatc agaatgagat ctgggagcgt
     2701 ttcaaggagg ctacaactaa aataaacaaa aagcatcagg aacatttcga aagcataaag
     2761 gagcagcaaa aaaagaatct tgaagccaaa gctgagctat gcgaaaaggc agaggaactt
     2821 gccgctcaaa atccaaaatc ggtaaaggag tggaataaac tatcaaagga actaattgag
     2881 ctgcaaaagc tttggaaaac aataggattt gcaaccaaaa aggaaaacaa taaggttttt
     2941 gagcgtttcc gtagcgcctg cgacactttc ttcaacaaga aacgtgactt tttcaaggac
     3001 attagagatg ttcaggtgaa caacctacag cttaaaaccg aactctgcct gcaggccgag
     3061 gcattaaagg aaagcactga ctggaaaaaa actacggaag atttaatcaa ccttcaaaaa
     3121 cgctggaagc aaataggtcc tgttcctcgt aaaaactccg atgaaatttg gaagcgtttc
     3181 cgcgctgcct gcgattactt ttttgagcaa aagtcgaagc attttgcatc ggtcgataac
     3241 tcatatgagg aaaatctcaa ggccaaggaa gcattgattg ccgaggttga agcctttgag
     3301 tttggtgagg atatggaaca aaaccttaac gccctcaagg aattccagag gcgctggacc
     3361 gaaatagggt ttgttccaat caaggtaaag gatgatgtcc aaaagcgttt ccgggctgcc
     3421 atcaaaaagc atttcgataa gttaaatgcc caggagggca agaataaggt ggtgcgtttt
     3481 caggctaagc ttgaggaagc taaggctaac cctcgtgcca tgtccaaact ccgccaggat
     3541 agggaacgca tgtttaaccg catgcgtcag ctggaaaacg acctcaccgt ttgggagaac
     3601 aacatcggtt tctttgctaa aaccaagaat gctgaggcga tgattgagga ggttaaacgt
     3661 aaaattgagc gggcaaaaga ggaaattgct gagctgagcg aaaaaattaa gcttattgat
     3721 aagttagaac agtaatactt aaggtcatgc cacagtcctc atctaccaaa tacatttttg
     3781 ttaccggagg agttgcttcc tcgttgggta aagggataat ctcagcttcg ttaggtaaac
     3841 ttttacagag taggggatac actgtaacca tccagaagtt ggacccatac ataaatgttg
     3901 acccaggaac actcaaccca tacgagcatg gcgagtgcta cgttactgag gatggagccg
     3961 aaaccgacct tgacctcggg cactacgagc gctttatcag tttgcccaca tcgcagctta
     4021 acaatgttac caccggtaaa atataccaaa cggtaataag taaggagagg cgtggcgatt
     4081 accttggcaa aaccgttcag gttattcctc atatcaccga tgaaataaag cgccggatta
     4141 agctgcttgg aacaaaaaat aagcttgatg tagttattac cgagattggc ggaacggttg
     4201 gcgacattga gtcgctgcca tacattgagg ctgtacgtca gcttaaatgg gagttaggcc
     4261 caaataattc agcttttatt cacctcactc ttgttccttt cctttccgca tcgggtgaac
     4321 taaaaactaa acccacacaa cactccgtaa aggagttgct cgagaacggt atccagcccg
     4381 atgtgttggt gctccgcacc gagaagcatt tgagcgacga tatccgccga aaggtagccc
     4441 ttttctgtaa tgttgaaccc gaggcggtag tggagtcaat tgatgttaaa accatctacg
     4501 aggttccact attgatgctc aaggagaaac tggatttaac catactccgt aagcttaacc
     4561 ttcctgcatc ggagcccaag ctcgacgact ggattaactt tgttaaccgg gttaagaatc
     4621 ccagccatcg cgttaaaatt ggattggttg gtaagtacgt tcaactacct gatgcttaca
     4681 agtcaatcat tgaatcgttc atacatgccg gggccgccaa cgactgcaag gttgagctgg
     4741 aactcattca atccgaggat attaacgcta gtaaccttag cgagaaactt ggtcatttaa
     4801 agggcattct ggtagcacca gggtttggcc atagaggtat tgaggggaaa attcttgcgg
     4861 ttaagtatgc tcgtgagaac aaagttccct tcctgggaat atgcttgggt atgcagtgtg
     4921 cagttattga gtttgctagg aatgtccttg ggttaaccga tgccaattca accgaaatgg
     4981 ttcgaactac gccttacccg gttatcgatc taatggaaga ccagaaaggc ataaccgaca
     5041 tgggaggaac catgcgttta ggtgcatacc catgtgtagt ggccaagggt tcaaataccc
     5101 ataaggcata caaaaaactt gagattagcg agcgccatag gcataggtat gagttcaata
     5161 ataagtactt tgagcaattc cagaatgccg gaatgatacc agtgggcatt aaccccgata
     5221 ctaaccttgt tgaaattatg gagttgtccg atcacccttg gtttgttggg gttcagtttc
     5281 accctgagta tagcagcaca gttcttaaac ctcacccctt atttgttgat tttgtgaaag
     5341 ctgcaataaa ttttaacaaa taaagtaagg tgtaaatgct acctttgcac tcactccaat
     5401 ttgttgaatt ttaaatttta aactgaataa tatttttgag atggatagga atacggttat
     5461 tggtttagtg ttgatttttc ttataatgat tggctttggt tacctcacac aaccatcaaa
     5521 ggaggagcgt gaggcatacc aacggaaagc cgattcaatt gcacgtgttc aggaggagga
     5581 gcaccaaaaa cgacttgccg aagagcaggc taaagcctta ctttccgata gtgccaagaa
     5641 acaggaggcc attactcagt tcggattatt ttccgatgcg gcaagcggca gcaacaagtt
     5701 tatcaccctt gagaacgatc tgatgagggt tacccttaca gctaaagggg gccgtattta
     5761 ctcggtagaa ctcaagaaat ataaaacata ttcgggtgaa ccgctcgtgc tctttactgg
     5821 cgatgaaaat acatttggcc ttcagttctg gggcgataat gttgccgtta aaacaaacga
     5881 tttattcttt acccctcaaa caaccgatag tattattacg gctaccaccg actcagtttc
     5941 agtaaccatg aggctagctg caggtggtaa ttcctatatc gattacattt atacaattaa
     6001 gcccggtagc tacatgctcg gctttgatat taagtttact ggcctggaaa agaatattgg
     6061 gaaccgtttg ggttacatcg atcttatttg ggattcaaag ttactacgtc ttgaaaaggg
     6121 tttggagaat gagcgtaact acacaacaat tgcctatcaa ttttttacag gtgattatga
     6181 ggagttcagc tctcggagca aagaggattc aaaggagctg aataacaaga ttaaatgggt
     6241 cgattttaag catcagtttt tttcatccat cattgtagcc aacgaccatt ttgttaatgc
     6301 cgatttaaag gcagttaata ttgacgatgg aaagcatgtt aagcagttta gcgcccgctt
     6361 ggctttgcct ttccaaaata aggaatcgga aagtatcggt ctgaaattct tctttggacc
     6421 caatcactat aaaactttaa agtcatacaa ttcgggtttt gagaaggttg ttccattagg
     6481 ccgtaatatt attcgttgga taaacaagta tattgttatc aatgttttta atttcctcag
     6541 ccggtacatt tcaagctatg gtctcattat tttactttta accatattta tcaagattat
     6601 tatttcgccc ttaacctata aatcatactt gagttctgct aaaatgaggg tgcttaaacc
     6661 ccaggttgat gaaataaatg caaagtatcc caagcaggaa gacgcactca agaagcagca
     6721 ggctgttatg gcgctttaca aaaaggtagg ggttaacccc atgggtgggt gtattcccat
     6781 tctaatccaa ttccccatcc taattgccat gttccgtttc ttccccgcct catttgagct
     6841 caggcagcaa agtttccttt gggccgacga cctctcatca tacgattcta ttcttcactt
     6901 acctttcagt atcccatggt atggcgatca tgtcagctta ttcacactgt tgatggccgt
     6961 ttcgcttttc atcacctcaa agatgaatac cgaccaaatg ggcgatacca atgcccaaat
     7021 gcccggtatg aagtttatga tgacctggat gatgcctata atgctgttgt tgtggttcaa
     7081 caactattcg gcaggcttaa gttactacta cttgctatcc aacgtaataa ccataggaca
     7141 aacctatctc ttccgccgct ttgtcgatga taaggctata cttgccaaac ttcacgagaa
     7201 tgccaagaaa cccgttacaa aatcaaaatg gcaacaaaga ctcgaggata tggcaaagca
     7261 gcaggaacag ctcaggaaaa agaaaggtaa atagataaaa taaaataaat aaagccagtc
     7321 atttcgactg gctttattgt tgtccgtgaa tttaaactgt aacagatgat aattatttga
     7381 ttgaattaca atgtttaact ctttataact ctgtgcaact ctttgaaaat tattgagttg
     7441 atagttgaca gttggtggtt gatagttgat agttgatagt tgattgttga cggttaacgg
     7501 ttaacggtat atttgtgtcg ctcaaatcta tttctaacct gtagacttat ttcaggacta
     7561 gatatttttc aggacgggtc aaatcctttc gtttcacgtt tcacgattaa cgattaacga
     7621 acataaactc tctctaaccc cctcatatcc acaattcctc ttttcacttt tcacttttca
     7681 cttttaacta actcaaactt cctcaaactt cctcaaactt cctctaactt cttctaactc
     7741 caccatattc cgactcattc ctactcattc cgactcaatc cgactcaatc cgactcattc
     7801 cgagcattcc gactcattcc gagtattccg agtattccga gcattccgac ccatttccac
     7861 ttaataatgg taaacaaccc ttttttaccc tccccccaac aacctgctta aaaacatttc
     7921 agaattcatc ttaattgcct atcttcgagg ccatataaat gcattcgccc atgcaaatac
     7981 ttattctaaa tatcaaaaag ttggttcagg tggaagataa accggcagtt tggaaagccg
     8041 gtaacgatat ggccaagctg aaaactattg ataatgcata tttactgata gacaaggggt
     8101 taattgccga ttttgggcca atgtccaaag ctcccgatat ttcatcgctt tcgggcgatg
     8161 tgctaattta caatgcgttt ggccgtatgg ttttcccatc gttctgcgat tcgcacaccc
     8221 atttggttta tgctggtagc cgcgagatag agtatatcga caagataaag ggtctgtcct
     8281 acgaagagat agctaaacgt ggtggaggta tactaaactc agccaaacgt ttagccgaaa
     8341 catctgaaaa tgagctttac aaccaatcgc ttgctcgcat ttacgagata attaggaagg
     8401 gaaccggagc cgtggagatc aagagcggtt acggcttatc caccgagagt gagttgaaaa
     8461 tgcttcgggt tattaaacgg ttaaaggaaa ctacgcctat aaccataaaa tcaacctttc
     8521 ttggcgctca cgcagtacct gctgaatata aaggccgaca aagcgagtat gttgatttga
     8581 ttatcaacga gatgatacct gtggttgcct ctgagggctt agccgattat attgatgttt
     8641 tttgcgataa aggtttcttt accgttgagg atactgagcg tatcctgatg gcaggaatca
     8701 agtatggcct taggcccaag ctccatgcaa acgaactcga tttttcaggt ggtattcagg
     8761 ttggcgtaaa gtataatgca ctctcggtcg atcatttaga gttcactggc gatgatgaga
     8821 ttaatgcact acttggctct gaaactatgc ctacacttct gcccggtgct gcatttttcc
     8881 tgggtatggt tgaccctccc gttcggagaa tgatcaacgc gggtcttccc attgctttag
     8941 catccgattt taaccctggt tcttctccat cgggcgatat gaagtttatc atgtcgctgg
     9001 gatgtatcaa actccgcatg ttgcccgagg aggttataaa tgcaacaaca attaacggtg
     9061 catacgccat gggtatttcc gatacacacg gaagcatttg caagggtaag gtggcaaacg
     9121 tgtttattac caagccaata cccagctatg agtatatgcc ctacgcctat acatccgatt
     9181 tgattgaaac ggttatcctg aatggcagta ttatcaacga taaatagttg gagtatgaaa
     9241 aaaaacttat tacttccaat agctatattg atatcggcat gtgctgaaat atcaattcct
     9301 acaaatttcc ctatcccgca aggttcaggg agtaagccac ttaccaatgc tgaggtggtt
     9361 caagggctta aagaggcatt agccgttggc gctaaaaact caacgggttt agcatcgcag
     9421 gttgatgggt tttacaaaaa ccccaagatt tttatcccct ttccccccga agccgaaaag
     9481 gttcgcaaaa cactggtcga tgccggtttc tccaagcagg tcgatgagtt tacactaacg
     9541 cttaatcgcg ctgccgagga ggctgccaaa aaggctttgc ctatttttac tgacgctatt
     9601 actaaaatgt cgtttactga cgccatggga atactgaggg gccctaataa tgcggctacc
     9661 gaatacttta ggaaaaacac atctgagcaa ctatttgccg cctttaagcc tcaggttcat
     9721 gaggctattc aaaaggtaaa gctcacctct tactgggagc ctttagcaac aacctataat
     9781 aggctaaccc ttttaactgg aggtaaagca gtaaacccaa atctcgacga ttatgtgacc
     9841 caaaaggcta tagatggtct ttttgtccta attgagcagg aagagttaaa aatccgtaag
     9901 gatccagccg cccgggtaac tgacatacta aaacgagttt tcgggaataa ctaaacaaac
     9961 aattaaggga tattaaactt taatctttta tcctttatcc tttatccttt atcctttatc
    10021 ctttaacctt tatcctttaa cctttaacct ttaaccttta acctttatcc tagaaccttt
    10081 gaactttaac aaacaccata accattatgt cactgaaaaa gttaattgag tgtgtgccca
    10141 atttttccga agggcgcgat atgacaatta ttaagcagat aactgatgag attgaagccg
    10201 ttgaaggcgt taagttaatt gatgttgatc cgggcaaagc tacaaaccga actgtagtta
    10261 catttgtggg aacacccgat gaggttgttg aggcagcatt tcgggcaacc cggaaggcaa
    10321 tggaactcat cgatatgagc aagcaccatg gtgctcaccc acgatttggc gcaaccgatg
    10381 tctgcccgct tgttccggtg gccaacatca ccatggacga ggtggttgag tatgcccgca
    10441 agctggctaa acgtattggt gaggaattag gaattcctgt ttattgttat gagtatgcag
    10501 catttgagga aaagcgccgt aaccttgcaa attgtagggc aggcgagtac gagggattac
    10561 ccaagaaact tcaggatcct gcttggaagc cggattttgg cccaacacaa tttcttccta
    10621 aaactggagc tattgcagtg ggagcacgca atttccttgt ggcatacaat gttaacctta
    10681 acaccacatc aacccgacga gctaatgcgg tagcatttga tatccgtgaa aaaggacgcc
    10741 caatgcgtgt gggcaaccct gttacgggta aaattgccac cgatgagaac ggcaatgaga
    10801 tatgggtgcc cggatcccta aaggcttgca aagcaattgg atggtttatt gatgagtatg
    10861 gaattgccca ggtgtcaatc aatttaacag atataaccgt tacccctatt catgtggctt
    10921 ttgaggaggc atctaaaaaa gcagcagaaa gaggcttaag ggttaccggc tctgagattg
    10981 ttggggttgt tccccttagc gccatgcttg aggcaggtaa atatttcctg cgaaagcagc
    11041 agcgttcgct tggtattcct gatagggaga taataaagat tgctgtaaaa tcaatgggtt
    11101 tggatgagct ttacccattt gatcccgata agaaaatcat tgagtatatc cttgaggaaa
    11161 aaaccggtaa aaaattggtt gacagaacgc ttgagggttt cactcaggaa actgcatccg
    11221 aatcgcctgc tcctggcgga ggttccatat cggcagccat gggcgctttt ggcgctgcac
    11281 ttgctaccat ggtagcaaac ctatcatcgc acaaacccgg ttgggacgat cgctgggaag
    11341 aattttccaa ctgggccgaa aagggcaagt tttacatgga gcagcttatt aaaatggttg
    11401 atgaggatac tgctgcgttc aaccgcataa tggaagcctt tggcttaccc aagggaaccg
    11461 acgaggaaaa ggcagcccga acaaaggcca tacaggatgc taccctatac gccatcgagg
    11521 tgccattccg cgtcatgaac ctttgctttg agtcgatgga ggttataaag gcaatggctg
    11581 aacatggtaa tcctaactcc gtttccgatg ctggtgttgg tgctttggct gcacgctcag
    11641 cggtaatggg ggctttcctt aacgtcaaaa tcaatgcttc aggcctaaag gataaggata
    11701 aagccaatga gttcattagt agaggtgccg agatagttaa gaaagctcaa caaatggagg
    11761 ctgaaattct atcaattgtt gaaagtaaaa taatggctta gatcattgta attaataaac
    11821 agcaaagggc gcctttagcg ccctttgctg ttttgaaggc aacccaacta aaatctaaaa
    11881 ccaaaagtaa gcagaactct actatcacgc accttgttgg taacgctagg aatattcaaa
    11941 tcatagaaaa catatttaac atcctgtaat gttcttaggt aggcaaaatc aattgatacg
    12001 ttgccagttc tgtagccaat accacctgaa atctgctgaa ttttggattt gtcgttaagg
    12061 aaacccgatt tgtagggcga tgggtagtag gcataacctc ctcttagagc gatatcccca
    12121 attttatact ctccaccaaa ccttaggttt gtgacactct taaaggtgtt tcccatttgg
    12181 ctatttaaat cccagaaagt gtcgccatcg ccaccgcggc ggaaacgcat ggtagagtaa
    12241 tcgagatgct caatatcaat gctaaccaat cccttttgca taattataat ggcaacactt
    12301 ccggtaatct tcataggtgt ttcaaaatga taatcatacc tacccaacgg gctgttgctt
    12361 tggaagataa cctctgtgcc agttgtgtcg aagtttgtaa ttatcgatga cgagaatgca
    12421 tcgtcgaaac tataaaatgt aggggtgtgg aacgatagcc caatgcgaac ttcaggtatc
    12481 ggagtataga tacctccaat tttcaggttg aagcctgagc ctttcgtttc tacatactgt
    12541 ttgtaatcca tgtagtagaa cctatcgccg tttggcctgg acggattgct ttcttttgca
    12601 tcttcagaat aggttgaatt aaatgtgtaa ttaaaatcag ttattcctaa cgatgcaccc
    12661 aaatacaact tatttgaaat attnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12781 nnnctgcgat ggggtgtatt taccattagt aatggctgag aaatagtcca tgatggaata
    12841 gttattgtaa tcgcctaggg taattgcgtt agagtggtaa tcgttggttc tgttgtagcc
    12901 aaatgataag ttgtatgcca ggatgccctt atcttcggat ttataaggct tgaacgacat
    12961 tacaaagcct aaattatcaa ggttaatcct attcttgtta tccgatgccg atacacctaa
    13021 aaaggttgat gatacttttt gtgttttgaa cgatggtgta atggtgaatt cgcctgattt
    13081 gtatacccct acaccggcag ggttaaccgc aattgacgaa aaatcggctc ccagtgctcc
    13141 aaaagcaccg cccatcgata caaaacgagc tgttccatta ttgtgggttt ggctaaacct
    13201 aaggccatcg gcaaggtttt gggcattaag gttgtaaccc agagttgata gggttgctaa
    13261 aaagccaatt gttaaagacc tgtatgtttt catgtttgtt tttttatgta attaatcttt
    13321 ttaagacaaa aaagggggag aggagaacct ctcccttgag gtgcatattt aactatctcc
    13381 ttctagtagt tgaagttccg cttgaactgc tacttgatga gcttgatgaa ctgcttgatg
    13441 atgaacggga taccgaagaa ttgctgctag aggaaccact acttgaacta cgggggcgtt
    13501 cataatcata gctacgggtt gaaggttgtg atgacgatgg ggtgctaacc ctgtttgggc
    13561 ggttgtaggt tggagtagta gttcgggtag gtgttgtagt tgatgggtta cgagtaatag
    13621 ttgtccttgt tgatgggttc tcaactctac gggtactgtt gtactcgcca gaattaccag
    13681 ttcgtgggcg ctcgtaggtt ggagttgtgc ccctgcgggt aggttccgat atgccaatct
    13741 ctcgggtggg ccgtgatgtt cttgaagatg aaaaatcgcc cgtgccgtca gatactcgag
    13801 ttgatgttcc atctcttcgt acaacagttt gattacctgt tgaggtagtt cggccctcgc
    13861 ccctgcgggt ttcataggtt ggcgttactc gtgtttgagt ttcacgggtt gtaacaactg
    13921 ggtttgtttc agcaggaaca tctgttcttg tcctacggga tgtaactatt tgtccatcac
    13981 ctggaatgac cgatgtactt cttccgccta catattgatc ggtattgttg gtcgtatttc
    14041 ctcttcggcc atagtagtac ctgttgctat tgggcgtgta cttgtcccag taataggttg
    14101 catcgtacca tcctgaccag tatgcgttac ttgcaacgta tggggagtag taccatggat
    14161 tgtagtacca agggttatag taccatggat cccagtaata ccctaagtaa tatgagttcc
    14221 atgggttata gccccatcca aacccgaatc caaagtagaa atgcgacctt ggccaaccaa
    14281 aagagttata aatgtaccag ggctcaaccc atacattact tcccattact acaactctgt
    14341 agtatgaagg atcgtaagcc tgggcgtaac gccagtcgtc ggaatagtaa acgctccagt
    14401 cttcaatacc atagcgtgga tcctcaaagc cccttaacct acgctcgtac gattcctggt
    14461 agctatcgga aagtattcta cggtaagggt ttggatcttc agccttgtaa ataacggtat
    14521 cgtttttgct gctatcttcc aatgccttta aggcttcagc aaatttgccg tccagtttat
    14581 tatactgttc cgatttagct gaaggtgcaa ctgggctaac gtttgcaact tgtacttttg
    14641 tagattttga attagttgtt ccatatattt catcatccca cgatgatgtt ttgctaacct
    14701 gcagccctgt agaacaggat gccagcatga aaactgcaag ggtaagcgct ggattgaata
    14761 atttggtttt catatgcacc tccatttagt tagtggttag ggtaataatt catttttatt
    14821 aattactttt gccagtgttt tcatattgat tacagcaaaa atcattccaa ttatttaata
    14881 tggcaaagga aattacaagc agaagcgaaa attattcgca gtggtacaac gacattgtgg
    14941 taaaagcagg actggccgat tattctgcag tacggggatg tatggttatc aaaccatacg
    15001 gttatgccat ttgggaaaaa atgcgtgacc aactcgatag aatgtttaag gaaacaggcc
    15061 atcagaatgc atattttcct ctattcatcc caaaatcgtt tttcagccgc gaggcagctc
    15121 acgttgaggg ttttgctaag gagtgtgctg tggttacaca ttaccgcttg aaaaacgacc
    15181 ctaacggcaa aggcgtagtg gttgatgatg aagctaagct tgaggaggaa ctggttgttc
    15241 gacccacttc cgaaaccata atttggaaca catacaaaac atggatacag tcgtatcgcg
    15301 atttgcctat actaattaac cagtgggcta acgtagtgcg ctgggagatg cgtacacgcc
    15361 tgtttcttcg tactgctgaa tttttgtggc aggagggtca taccgcccat gccaccatgc
    15421 aggaagccgt ggaggaagcc gaacgtatga taaatgtgta tgccacattc gcccgcgagt
    15481 ggatggcaat gccggttgtg gttgggaaga aatcggagtc ggagcgtttt gccggggccg
    15541 ttgatacata tgccattgaa gcgcttatgc aggatggcaa ggctttgcag gctggaactt
    15601 cgcatttcct tggccaaaac tttgccaagg ctttcgatgt aaagttcctg aacaaggagg
    15661 ggcaacagga gtatgtttgg gctacatcgt ggggtgtttc tacccgcctt atgggtgccc
    15721 tggttatgac ccattctgac gataatggtt tggtgctgcc tccaaaactt gctccaactc
    15781 aggtggtttt tgtgccaatc tataagagcg atgaggagtt atcagccatt agtgaggttg
    15841 ttcacaagct aagatctcaa atgcactcca aggggattac cacttactac gacgacaggg
    15901 ataactacaa gcccggcttt aaatttgcag agcatgaaat gcgaggtgtt ccagtaaggg
    15961 ttgcaattgg cccaaaggat atcgaaaagg gaacagccga ggtggctcgt cgcgataccc
    16021 ttcaaaaaca atatgtgcct caggagcaac ttgccgatac tatagttgct ttacttgatg
    16081 atattcagca aaacctctac aaccgggcct tggctttccg tgagaacaat accgttaagg
    16141 tagattccta cgacgaattc aaaaagatag tagcagaaaa gggaggcttt gttctggctc
    16201 attgggatgg aactcccgaa accgaggctc agattaaaga ggaaacaaaa gcaactattc
    16261 gctgtattcc gctcgatatg ccagaggagg aaggaaaatg cattttaaca ggaaaaccat
    16321 cgaaacgtag ggctattttt gctcaagcat actagtaacc tatgagatgt ttaattttaa
    16381 taaaatgggc tgcaaaacgc agcctttttt gctttgttta cttttaatat ttaatgtata
    16441 tttgaaattc ttaaactgaa tatttatgga aaaccaaaac acacagcaaa aaattcaaaa
    16501 tctacttacc gagaaagccg ttgtaaaaca gaaaatttat gataataccc ttgaggtttt
    16561 tggattgatt aaggaagttc tcaaagaaat tgaggaagat tttaacgata agcttagggg
    16621 gtacgataaa agggttaagc tggagtacaa tgaccgggga aaatatgaag ccgaattaaa
    16681 ggtggctggc gatttgttaa tttttagcat gcataccaac gtatttgagt ttgaccgcga
    16741 tcatagtata tggaaactat cctatgtcac cgataatgtt ctcaattcat attgcggaat
    16801 tatcaacata tataactttt tggccgattc ctttaaatat aataggctcg aagaccttgg
    16861 ttatctgatt ggccgaatat ttgtaaaccg tgagtatcac tactttgttg aagggaaacg
    16921 ccaaatgggg ttcctttaca acaatttcgg ttcagcggtt atcgataaaa aatcgctaag
    16981 aacaataatt gaaacggcca ttatgtatgc attggaattc gacttgctgg ttccaccata
    17041 cgatgtggtt aaaatagctt cggtagtgca gctaaatact aaaattgaga atgctaagct
    17101 gcaaaccggt aaacgtttgg gtttcaggtt taactccgat gatgttttgg aggaaaagga
    17161 acaataatct atcaatataa attttattat gaaagggaaa acattactac ttgcagctgt
    17221 tgcaacgctc acagtaattg gttgcaatac aaaaaaaaca caggagaccg aaatgcagaa
    17281 aaaggttaac gaatttgctc cggttgagct tagggttgat ataatcagcc tttccgataa
    17341 ggaaaagcaa atgcttcctt tactttttga gattgctgaa attatggatg acctattttg
    17401 gctacaaacc tatggcgata aggaagaact gcttagcaag ataagcgatg aagcaactcg
    17461 cgactttgta atgataaatt atggcccatg ggaaaggctt aacgataaca aaccttttgt
    17521 tgagggttat ggcgaaaagc cccttggcgc aaatttttat ccgaaggata tgactcgcga
    17581 ggagtttgaa gcctttcagg ctcccgataa aacaagccta tatacaatta ttattcgtaa
    17641 taccgatggc aagcttgagt cggtaccata ccatgttgct tacaaggaaa aaattgacaa
    17701 agccgccgat ttgcttcgta aagccgctga acttgccgaa gatgaaggac tcaaaaaata
    17761 tctcaccctt cgtgccgatg ccttgcaaac cgatgactac tttgccagcg acctggcatg
    17821 gatggatatg aagaccaata atatcgactt tgtggttgga ccaattgaga actacgagga
    17881 tgctctgttc aactataagg ctgcttatga agcatacata cttataaagg atttagactg
    17941 gactcaaaag attaaccgtt ttgctgcact actcccaaaa cttcaggaaa gcttaccagt
    18001 tgaaccggaa tacaaaaaag aaaagcccgg tatcgattct gatttaggtg tttatgaggt
    18061 tgcatactat gcaggcgatt gcaattctgg aagcaaaacc attgctatca atttacccaa
    18121 cgatcctcgc gttcacctgg aaaagggtag ccgcaagctg cagcttaaga atgctatgaa
    18181 agctaagttt gaaaaaattc ttctgcctat tggaaatatg ctcattgccg aggagcagcg
    18241 tgataacatc aagtttgatg ctttttttga gaacgttatg ttccatgagg ttgcccatgg
    18301 gttaggtatc aaaaaccttg ttaatgggaa aggcacggtt cgtgaggcat taaaagaaat
    18361 gcattccgct ttggaagagg ggaaagccga tattcttggc ctttacatgg tatcgcagct
    18421 tgcacaaatg ggcgaactgc ccgaaaagga catgatggac aactatgtta cctttgttgc
    18481 aggtattttc cgttcggtaa ggtttggtgc agcttcgtcg catggaaagg ctaacatggt
    18541 ttgctttaac tacctgaaag aacgtggcgc ttttgagcga attcctgaaa caggaaccta
    18601 tcgtgtcaac tatgaaaaga tgactgaggc catgaattca ttggccaagg acattttaac
    18661 ccttcagggt aatggcgact atgctgccgt aaagaaactt tttgaagaga aaggattcat
    18721 taccgatgaa cttcaggccg atctgaacag ggtggctgag gctaatatcc cacgtgatat
    18781 taactgcatt cagggaaaag agttgctggg tttataaacc taaaaattaa ggtcggatgg
    18841 tttgctttcc gacctttttt attctatttt tgaaagcaat ataaaatttt tggagtatga
    18901 acctatcatt taatttcatt aaatccgtct cgaatattta cgacgaaatg attgataatg
    18961 gattctccct ggtttacctt ggggaattta gccatgagat cacaaaaatg tttacatcga
    19021 tggcggaatc ggatatggaa aaaaatagtg aggaacgctc ggtccagcgt aaggtttacc
    19081 acgttatggt tgaaactctc cagaatatga ataagcattc cgatgagatt caggagcgta
    19141 atattggtaa agggttattt atcataggta aaaaacacga tacatactat attattactt
    19201 ccaacaaggt agcccgaaag cataaagatc accttgaaaa cgcactgata accgttaata
    19261 gcgcaacacc cgaggagctt aaggagatgt ataaacggca aattcgcgaa ggaaaccttt
    19321 ccgataaggg gggggcaggc ttagggctaa ttgatattgc tcgtaaaaca ggtgaaccat
    19381 taaactacca attccttcaa ctcgacgatc aaaacttctt ctttatcctt aaagttgaaa
    19441 ttaatgccaa aaaatttgct aaggagtaag cttatttctt gatgaatgac tgattttaag
    19501 ttgctttcat atactcgtgg taaaaccatc attgcattag taatgatggt ttatttttca
    19561 ttttttggat ttattaatta ttcatctttt gcacaatctg atagcaaccg ggttagttct
    19621 gatagtatta aaaagaaaga ggataaaatt tctcaaaccg tatataaatg gtttggccgt
    19681 cagaatgtta atgttgcacc attaaccaat gaggaaactg ttaaccgatt cttgaagcat
    19741 gaaggcaagg taatttcaca gattagtata atacgttttg atgcgtttgg atactcggtt
    19801 tacgatacta ctattaaacc agagaggaat attgaaaaat ttgggaatag cttgcattta
    19861 aaaacctgga aaaaaattat tcaaaacaac ttgcttttca aggaaggcga taaggttgtt
    19921 ccattggatt tggctgaaac tgagcagttg ctgcgtcaaa gtaacttctt tgaggatgcc
    19981 aatattttag ttcaaccctt gagcgattca agccatgtgc atgtttatgt gataaccaag
    20041 gatatttgga caatatccgc tggggtgcaa tactcatccc ctgaaaagat gaaacttgct
    20101 ttatcggaaa ggaactttgc gggtttaggt attcgagcta aagcaatagc ttattataat
    20161 aagactttta ccgataaatg gtcgtatcaa ggggagtttg atgtaccaaa ctattttggt
    20221 tcatatattt ccagtagctt ttttgtaaga aaaggtcatt cctatgagac ttaccttatg
    20281 aggtttaacc gtgatttctt tgcatccaag actaagtatg caggaggtat tgaactttta
    20341 aagtccgacg aaccttacag ggttttcacc gacgattcag tagtacaaat ttcatatcag
    20401 tcgcaggact ggtggattgg ccgatcagtc agggttagta gacgctctgg taccagtgcc
    20461 cctctaaatc tggtttttgc attacgttac tataggcgcg attactttca atcaccaccg
    20521 gtttctattt acctaaatcc aatatttcat tcggctgaaa attatgttgt ttcgctgggg
    20581 ttatccaacc aaaacattta tcgctccagc ttatattttg gatttgggaa cactgaggat
    20641 ataccggttg gttttaaaat tcaggccaca agcggtattc agcaaagtac atttcagcgt
    20701 cgttttttga ttggagggga gctttcagca gccgaggtta caccgtgggg ttatttgttt
    20761 ctatcgtcaa ggctaggggg ttatcttgct gaggggccta aggtagagca ggctgttatc
    20821 aaccttagag cacagtactt ctctaacctt taccacattg gccgtagcga tattcgccat
    20881 tttattaggt acgattttac tcgaggggct gcccgtttta gtggtgaaag ggagtatgtt
    20941 gttttaacaa acaactatgg tgtaagggga cttaggagtc ccgaccttta tggccaaaca
    21001 cgattgatgt ttaactttga gtccatgtta tattcacccc attacatcta cggatttaga
    21061 cctgcttttt ttgtttttgc cgattttggt atggtgggtg agtccgatga gttaatctac
    21121 caaaacaccc tgtatagtgg gtttggagtg ggtttaaggt tgaagaatga aagtttgata
    21181 tttcctacgt ttctttttcg tattggttac taccctaagt tgccacccga tgccgaagtg
    21241 gcatactggt ttatttcaac cgagaaccga aggaattttg agcagtttag gattaaagaa
    21301 ccatacattc ttcaatatca gtaatattca tacttaaaaa cttttttcat actgttttgt
    21361 ttcttaaaca gcataaaatt ttgatgtatg acaaaaacta tacttgtaac cgggggaacc
    21421 ggttatattg gttcatgggt tgttaaggaa ttgcttgaaa agggttatcg cgtaagggta
    21481 cctgttaggt cgtttaacag aaaggacaag tacctgcatc tgcagagttt gccaaattcc
    21541 actaatttac ttgaactttt tgaggctgat ttgctgattg atggagcttt tgatgaagca
    21601 gctgagggta ccgatgccat aatacatatg gcctcaccat ttactcttcg gtttaaagac
    21661 ccgcaacgcg atttggttga ccctgctgtt aagggtacaa gaaatgtttt gggtgctgca
    21721 agcaaatcgt cgacagtgaa aagggtagtg ctaacctcga gcgtggctgc tgttcatggc
    21781 gataatatcg acatggagga acaaggatta accgagttta ctgaggaaaa ttataacact
    21841 acaagttcat tgaagcatca accttactcc ttttcaaaag ttcaggctga gagggaggca
    21901 tggcgaatta atgcggaaca gagtaaatgg gagttagtgg taataaaccc cgctttggtt
    21961 cttgggcctt cactcgcagg aaattccaat tcggaaagcc ttcaaatcat gaaggatttg
    22021 ctttccggta agtatcgttt tggcgcacct gacctttact tcggttttgt tgatgtacgc
    22081 gatgttgcta aagcacatgt gctagctctt gaaaaccctt ctgtttctgg tcgatttatt
    22141 attgctgaac gagtggcaaa tttcatggaa ctggttgcaa ttatcaacaa taaattccct
    22201 ggtcagttta aactccccaa aatggtcgcc cctaagtggt tactttactt tgttagccct
    22261 ctttttggcc tttcacctcg gtttgtagcc cgtaatgttg ggtatcgaat tgcgttcagt
    22321 actagtagaa gcaggcagga gttgggtttg ggatataccc cttttgaaca tacagttgtt
    22381 gatatggtga accagttcaa gaaaaattaa accatcccca ctcttcatga acccataatt
    22441 ggctaccttt gcgcttatgc gcagtaaata catccatagg gtaagcaatt ttgataggtt
    22501 tgccaaaaat ctggcgtatg aggttgaaaa caccgatgtg ggtattcttc ttcggggatc
    22561 cgataggttc actattccgg aaaccaattt taccagccgt tttcaacttg ctgctgcatt
    22621 aggtattagc tcggcttgca gtccaaattc taatcatttt gatgaactcg atgagttcct
    22681 aagcgctcat aatgattggg tttttggtta ttttacttac gatcttaaaa atcaactcga
    22741 acatttacag tccaataacc ccgatggagt tggtttaccc gatatgtttt tctttgtacc
    22801 ccggatacta atactggtca atggtgaaat tgctgagttt cattaccttc agcaagaagt
    22861 atcacaagaa gaagtaagtg atttgattga taggctagaa ggtgacttgg aggaattgga
    22921 ccacaatgtg tcggttgttt tccgttccca ttattcctat aacgattact tgaatacggt
    22981 tagcgcagtc aagcagcaca ttgcccgggg tgatgtttac gagttgaatt tatgccagga
    23041 attctttgct aacaatgtaa gaattactcc ttatgttgtt ttcaataagc tttacgacag
    23101 gtcgccaacg ccttttggcg catttcttag ggtaaacgat aagtttctga tgtgtgccag
    23161 cccggagcgc tatttgcgtc gcgatggtga ttggctattc agccagccaa ttaagggtac
    23221 agcaccgcga atggaatcgg acgagtctga tcagcaggag atatttcgac ttaaaaccga
    23281 tcctaaggaa cgtgctgaga atgtaatgat tgtcgacttg gtcagaaacg atttatcgcg
    23341 aatagccaag cgcgggagtg ttagggtaaa tgagctttgc gggatttaca catttccaca
    23401 ggtgcaccag atgatttcaa ccgtgtcgtg cgagctggcc gatgggctgc gcccaaccga
    23461 tccttttaga gcaacatttc cgatgggttc aatgactgga gccccaaaag ttagggtaat
    23521 ggaactaatt gagaattttg aacgttccaa acgcgggcta tactccggtg cagttggtta
    23581 ctttatgccc aacggcaatt ttgattttaa cgtggtaatt cgtagcttgc tttataatag
    23641 aagcagtggt tatttgtcct tcacagttgg tggggcaata acctttaagt cgcaacccga
    23701 ggctgaatac agggagtgct tagttaaggc aaaagccatt ttagaaaccc taaatgctca
    23761 gatagatgct gcaaagtaaa atgatcgatt ttgttcgtga gcaccagatg gcaaatgacc
    23821 aggataaaat cctggtaggg gttagtggtg gtatcgactc gatggtgcta ttgcacctat
    23881 tggtccatgc cgggtatagt gttggtgttg ctcactgcaa ctttagcttg cggggcgatg
    23941 aatcggatgc cgatgaacat tttgtccgtt accattgcct gatgaatggc ttaccggttc
    24001 ataccattaa gttcgatacc ttggaatatg ccaaggagaa tggcttatcc attcaggtgg
    24061 ctgctcggga attgcgttat gcatactttg atgagatttg taaggaacat aaatacaccc
    24121 gaatagccat agctcacaat ctaaatgatt ccgttgaaac agtgctgcta aaccttagtc
    24181 ggggaactgg tttaagaggg cttaccggaa tcaaacccgt aaacgggaat atcattcgcc
    24241 cactgctttt tgctacccgt gttcaggttc aagagtatgc taccttacat aacatttcgt
    24301 atcgcgagga ttccagtaac gcatccgata agtacaagcg taactatatt aggcacaatg
    24361 ttttgcctct tttgcgtgag ttaaacccct ctgtcgatat ctcaatttat caaaccgcac
    24421 agcatcttcg tgaagctcag cttattgttg atagcttttt gactcaatta cgtaaggaaa
    24481 ttgttaatga aaatgggggg gtattctata tcgatattga aaaactgaag aaccaggccg
    24541 ctgtcaatat ttttttagtt gattttctgt cggactttaa tttcacccct gccctgacta
    24601 ctgaggtttg cgaactatta gaatcgtcaa ttggcaaaag ggtagagtcc gatactcatg
    24661 taattttccg cgataggaat caccttattt tacagcaaaa acaaaagagg caaagcgttg
    24721 aatgtcagat tgatgtcaac ataactcaaa ttgaccaacc tgtaaaatta agtttgagta
    24781 aacttgatta ctctcaaggt tttgtgtttg ggaaagatac aaaatacgca tatcttgatc
    24841 tggaaaaact caagtttcca cttaccatca gaccatggca acctggtgac cggtttatgc
    24901 ctttcggcat gaccagcttt aaaaagatta gcgatttcct agttgacatc aaaatacccc
    24961 ttccagataa ggaaaaggtt tttgttctga cttcggaaaa ccaaattgta tgggttgttg
    25021 gttaccgaat tgataatagg tttagggttg atgagaatac ccaaaagatt ttacttatca
    25081 aatttgatga gtgattgcat ggttattcct caaattcatt caattccaac caacgcattt
    25141 ctttatcttc gagtagtttg gagatttcac taaacctatt tgaaatgtcc attagcagct
    25201 tagcatcctg aatgccaccg ttgagcaaac cttcaatttc tttcttttcc ttttccagct
    25261 ttactatttc atcggtaagt ttttccaact ctttcttttg ggcaaaggtt aacttgctct
    25321 tcttcctttc aggttggggg cgtttgctat cagttttccc ttttgaggag gtttgttgtt
    25381 cggcattact tttttcctgt tcctttgccc attcacggta ttcagtatag ttgcccggaa
    25441 atccgctaat ttccccattg ccctcaaaaa tcaacagacc atcaactagc ttatccataa
    25501 aataccggtc gtgcgaaacc acaactacta cgccaggaaa gttaatcaga taatcctcta
    25561 aaacattgag cgtaaaaata tcgagatcgt tggtcggctc gtcgagtatt aaaaagttag
    25621 ggttttgcat aagcactgtt agcaggtata gcctgcgctt ttcgccaccg cttaacttac
    25681 gaaccagagt gtgttgcctg tcgggtgtaa atagaaagtt atttagaaac tgcgatgcgg
    25741 ttatagtttt accatttgca agggtgatta cctcagctat ttcgcgaaca acgtcaataa
    25801 ccttaacatc ctcgggaatg ttcattccgc tctgtcggta atatgcaatt ttcagagttg
    25861 caccggggtc aattgttccg gaatcgggag ggagtgttcc tgtaatcagg ttaaggaagg
    25921 tagttttacc agtgccatta gcaccaacta taccaagccg ttcaaagcgg ttgaaggtgt
    25981 agctgaagtc ttttagaatt actttactgt caaagctttt tgaaagatta tggacctcca
    26041 ttattttttt tcctaggcgc gaggcgctta cgcctatttc aacatcattg gaatgaattt
    26101 tttgtagtgc agtttcgcgg agcttgtaga atgcatcaat tctatacttg gcttttgttc
    26161 ctcgtgcttg cggcatgcgg cgcatccatt cagtttcccg tcggagtaag ttgcttgctc
    26221 tgtcaatttc tgccagcttt gccctaattc gctcatcgcg tttctcaata aaataggaat
    26281 agtttccgtt gtacgtataa acctgctttt gatctatctc aataatatcg gtgcaaacgc
    26341 ggtcaaggaa atacctgtcg tgggtaacca tcagcactgt tttttgcgac cgtgttaggt
    26401 aatcctccaa ccattccacc atttcaaggt cgaggtggtt ggttggctca tcgaggataa
    26461 gcaggtcggg gttgcttatc agcaccgcag caagtgcaac tcgcttgcgc tgaccacccg
    26521 aaagcagttg aacgggttga tttatatctg taagctgaaa aactgagagt atttgcttgg
    26581 ccttttgttc cctatcccaa atgttaagcc tgtccatctc atcaatggca ttctgcaatg
    26641 ccattttatc ggttgactca atagcttttt cgtaattacg tatggcaacc gaaatagcat
    26701 catcggcatt aaaaacattt tctaaaaccg attttgataa attgagttga ggttcctgtg
    26761 gtagatagcc aacgctaata tcgttacgta gggttacttt tccggaatcg ggtgtgtcat
    26821 atcctgcaag gatattaagc aatgtgcttt tacctgttcc atttcttgct actagggctg
    26881 tgcgctggtt ttggaatagg ctaagcgata catcgcttaa tacttccaaa tcaccaaacg
    26941 acttgcttac attttcagct tgtaggtaaa ttaatgccaa agtattcttt ttttgagttg
    27001 caaagttagt aatttgtgca acatttgctt aaaccgaatt gccatgaatt aacaaatcca
    27061 tacgggctga taaaaatgga gatagtagga tttttagatg aatgagtcgg aatgaatcgg
    27121 aatgagtcgg aatggacaga atatgatgaa gttagaggaa tagtggaaag tgatctgtta
    27181 aactgaatta taatgaattt gcaaatccat acgggctgat aaaaatgctg attcttaaat
    27241 attttatgag gtgatttgcc agtggaaata aaaaaggttt aatcaattta atctaatttt
    27301 ggagttaaag agaaatatga tgaattcaaa aaaggaacta taccgttttg taatagagta
    27361 ttttgagaaa acccgtccca tagccgaaac agaattgcat tactctaacc catatgaatt
    27421 gcttgtggca gttattctta gtgcgcaatg caccgataaa cgtgtaaatc taataacacc
    27481 aaattttttc aaaagttttc ccgatgctgt gagtttagca aacgccagcg tggaagatat
    27541 ttacaaaata ataaaaagct gctcataccc aaacagtaag gcaaaaaatc tgctaggaat
    27601 ggccaaggtt ttgatgactg agtttaatgg cgaggttcct tctgatatca atctattgca
    27661 acgccttccg ggtgttggcc gtaaaacagc caatgttatt gcatcggtag tgttcgataa
    27721 accagccatg gctgttgaca ctcatgtgtt ccgtgtagca aataggattg gtttggtaag
    27781 caacgccaag accccgcttg ccgttgaacg ccagctgatg gaaaacttcc cccttgaact
    27841 tattcctaaa gctcatcact ggcttatact tcacggtagg tatgtgtgta ctgcacggaa
    27901 acccaagtgc acagaatgtg gtttaacact ggtttgtaag gaatacaaga aaatcagtaa
    27961 aaaagcagaa aaataaaaag ggacgcattt gcatcccttt ttaacttatc gggtaggtat
    28021 taaagcttta caattgcacc tattgtaata aaccctgtgg ttgcaatggc atcgaggtta
    28081 gcgccaactc caaaagtatt ggtgatgttg gttgtgttgt tatttttaac cgagacacgg
    28141 ttaaagttaa ggttaagtcc accaattact gcttcaagct caactttatc agtcaattta
    28201 tatgaaatgc ctggaaatgc cgatatccca atggtggttg ttttagggcc agttgtagtt
    28261 gttgaaccct ccttgtgctt ttcaacacca tacgaaaaac ctaaattccc ttgagcaaaa
    28321 accgaaaatt tgtttaggga aaaggcgtag taacgggcaa acggcgttaa cccaaatgtt
    28381 gtagttctat caatttcttc aggggtgcca ggcgttttct cactttgtag gttaaaacct
    28441 agtatggctc caactgcaag cttttcggat aggaacattc ctgctttggg ggctagtgag
    28501 aagctggttt gtgtaacctt gtcaacggaa gtgttgccat tttcaatttt tccgccagtg
    28561 gtgttaaatc caacgctacc acccacaaaa atttgagcaa acagtcctgt tgtggcaaat
    28621 aataataacg aactaattac tactcttttc atggctatgt ttttaaaagt tcgcactaaa
    28681 ttattgcttt tttaattatt tgccatgtta aatagtgttg tgttcaaaat ttattgaaat
    28741 tacattactt gaaattgaat ataaagggca gaataaaaac cacaaaaatc aaccatattc
    28801 catagtaggg taaaaattta gccgttactt gctcaataat ggttaagtgt tgtttttttc
    28861 gtatcactct caataaattg gttaagatta gaattaaatg ggtgaaaaat agaaggttcg
    28921 atcctattac agctaggcgg ttaggtgtta tgccatactc cgtaatccta taaagaatag
    28981 ccagtaacgc tacaacattt attagcaaag ctattgttga aagagcagtg aaaatgattg
    29041 tgtaataatt atttgttgag ttgttaactc tttctgaaat tgaaaagatc accaatgcaa
    29101 caactccaat aagcattgca ttgaaaataa ttagaaaatc cctattaaca aaaggattgc
    29161 ttccttttgc tactatgctg ataaggtata ttatgagtgt tattagtatt agtgggctaa
    29221 aaatattggc aactatagtg acaattttat ttgataggta ttcgaattta ttaatcagat
    29281 atgtgcaaat tattggaacg gaaactactc caggtataac aattagttca aaataaattt
    29341 tttcaacatt tattccaatt gcgctaaaca acccaatggt aaaagcagtt aggatcattc
    29401 ctgctattgc aaaaatgcta aacaaaacag ctaagtcgcc attatacttt ataaactcaa
    29461 ttcgtttttc attgtttttt agttcgaact ctgagaatac tatgccaaaa ataaaccaaa
    29521 gaagtatggg taaatggatg tatatcaggg tggtagagct actttccgtt tttggcagaa
    29581 catttacata aattgcagaa ataataaaaa atccaacgat tgctgcagtt ttttttaggt
    29641 tgatattttt tagtatggcc gtgtaaaggg tgactcccaa gagagccgtt agcgcaaggt
    29701 tccgttcgta aaaattattt tcatgtgggt taaacccaaa caaccaagga attaacatga
    29761 caatactgac tatcaaacag gaaattaata catatgcaat ttcttttagg tttacattga
    29821 acagtatttt attatcggcg gttagcctgg taaaccaaaa ttttgctaat ggaatgcttg
    29881 atatttcaga gtaaatgctg aaaaaggctt tttcaaaacc ttttctgttc tttctgtaaa
    29941 gttgctcaag cacttcagga ttgtcgaggt tgttcctaat tgagttttcc attgtgaagc
    30001 gatttttttg gttaacgcca atataagtaa attattgtga agtgagttag aatgaattcc
    30061 caaaagtgat gaacctcaag ttagttccgc tgcgctccat gatccctccg gcaatgctga
    30121 ttatggttgc ttttttgaac aaagaaaacc agtcctgttc gactggctta accaagtgac
    30181 cccggaggga ttagttccgc tacgctccat gatccctccg ggcctataag ttagtgtatt
    30241 gttattcaag aaagaaagcc agtcctcttt ggctggcttt ttttaagtga ccccggaggg
    30301 attcaaaccc ccgactttca gaaccggaat ctgacgttct attcagctga actacggggc
    30361 catttatttc ctgcaaagat atagctgttt agccattttt acaactactt ttttagtgtt
    30421 acctttgttt ttaatgtaaa caactctaag aatgaaagcc aattttgtta tactgtcatc
    30481 gttgatttta ttgagtttcc ggttaagcgc acaggttgtg tttgttgttg attcttttcc
    30541 ggcaagaact cggcccgatg acacaatttt tatggcatcg gaactaaatg gatggaatcc
    30601 gcatgatatt agttgggcct ttaaacgaac tacgaaccgt tatgaactga aattaacgca
    30661 tgtaaaggat acctttgagt ataaactttc acgtggcagt tggcaacttg ttgaggtgga
    30721 ctccaatggg ggcgatattc gaaacaggat ttatttccca cctaccgata ccgttagtat
    30781 taaggtgaat ggatggcgcg atatgtttga ggccgctcaa cgaaaatcca cagcttcaac
    30841 taatgttagg ttcttaccct caacccttga gatgaaacag ctgaatcgcc gtagagcggt
    30901 gcggttatac ttaccgccaa actacagcaa aagacagcag ttccctgtaa tttacatgca
    30961 cgatggccaa aatgtttttg acgaggctac ctcatttgcc ggggaatgga gggttgacga
    31021 aataatggat agcctttact acagctgcgg ctttgccgcc attgttgtag ctatttataa
    31081 tgatagtaag gagcgtataa acgaatactc accatggcgt aacgactccc ttggcatggg
    31141 tggcgatggc gataagtatg catactttgt tgccaaagag cttaagccat ttatcgataa
    31201 aaactatagg actaatcctg aacccaagtt taatgctgtt attggtagct caatgggtgg
    31261 attaatttcg ctttatatag ggctcaagta tcccgatgct tttggtaggg tgggagtgtt
    31321 ttcaccttcg ctctggtttt cgcctaaagt atttgagtat gtttcaaagt acaagcgtag
    31381 ggctgatcag aaaatatatc ttttggctgg tgaaaaggaa agcgaaacct tagttgagga
    31441 tatgaaaatg ctggaacacc acctatacaa agctggatat aaggatgagg attatgttaa
    31501 aatgaaaatt gcacctgatg gtcgtcattc agaatggttt tggagccggg agtttactga
    31561 ggctttggag tatctgtttg aaataaggaa aaactaactc gataattttt aaaatgcgtt
    31621 ggcctgtttt ggttatccaa tggaaaaagt gtccagcaaa tatttctttt ataccttgag
    31681 ttactaacag taaattattt atttcatcat tccagtaatc aatgtcgttt agttaacaaa
    31741 aataaacatg cctgtcatcc tgagcgcagc gaaggatctc ttaatcgtcc ccatgagacg
    31801 tttcacatag ctcaacatga caatagcttc acgaacaacg attcacgaac aacgaacacc
    31861 gctt
//
LOCUS       DAOSAL010000024        30977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_1084_length_30977_cov_49.816118, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000024 DAOSAL010000000
VERSION     DAOSAL010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 30977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30977
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1084_length_30977_cov_49.816118"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..591
                     /locus_tag="PK049_05070"
     CDS             154..591
                     /locus_tag="PK049_05070"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOK60960.1"
                     /translation="MESITIRDFQPSDYEGLSELWLLTNLGNPARGDNLEIIQQSIDL
                     GGKLLVAVNSDGMVIGTSWMTFDGRRIHLHHFGVHPNYQRQGIGKMLTRESLRFVKAK
                     GYQVKLEVHRNNIAAINLYKQFGFQYLGDYDVYIIRDLNSISI"
     gene            complement(691..1320)
                     /locus_tag="PK049_05075"
     CDS             complement(691..1320)
                     /locus_tag="PK049_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOK60961.1"
                     /translation="MKTLPFLVAVLLSLVSNGQNDSITKLPEPSRVKGKNIMEAFANR
                     ASVREFDTQELDLQEIADLLWAANGINRPAEGKRTAPTAMNTQDIDVYLVNSKGAFLY
                     DAKAHELVLISSGDYRSAVAGSQQNFAKAPIFLVIVSDVARFQRGDESQKMTWAAMDA
                     AIVSQNINLFCAGVGLKTRPRVWMETEKLKEILKLRETQRPMMNNPVSR"
     gene            complement(1333..2367)
                     /gene="mltG"
                     /locus_tag="PK049_05080"
     CDS             complement(1333..2367)
                     /gene="mltG"
                     /locus_tag="PK049_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOK60962.1"
                     /translation="MKKFVKFGLVIALVGLAVGVLLLLRLYFTFRATNVAVSKETLIY
                     IPTGSNYNDVLNILDKNKVLKSIDRFKRVAQYYDYEKSVKPGCYAFKPGMSNSSMVKM
                     LKYGHQKPVKVTFTGMRTWQQLAQRISSQIEADSVSLINLFTSDSVAFNRGFTPSTFM
                     AMFIPNTYEFYWNTHALGFINRMYKEYNRFWNESRIHKADGIGLSPVEVAILASIVEE
                     ETNVKSEMPIIAGVYLNRLRKGIPLQADPTVKFAIGDFTIRRVLTRHLAVDSPFNTYK
                     HKGLPPGPIRIPSVIAIDAVLNAQKHSYLYFCASPDFSGRHIFAQTLAEHNRNAAEYR
                     RALNMERIYR"
     gene            complement(2457..3650)
                     /locus_tag="PK049_05085"
     CDS             complement(2457..3650)
                     /locus_tag="PK049_05085"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010801692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOK60963.1"
                     /translation="MEKVSARIAALEESQTIAMSKKSRELAAQGIDVISLSVGEPDFF
                     TPDFVKDAAKKAIDQNFSFYTPVAGYKDLLEAICGKLKRENNLEYQPDQIVVSGGAKH
                     SLSNVLMCIVDKDDEVIVPAPYWVSYVELVKLAEGKSVVIETSLESDFKITPKQLEAA
                     ITPKTRALLLCSPSNPTGSVYTYDELKAIAQIIEKHPNIIVISDEIYEHINFIGKHES
                     IAQFDSIRDRVVIINGVSKGYAMTGWRIGFSASAKWIAKACEKLQGQQTSGVCSIAQR
                     AAIAAFNSDGEYTRQMREAFRRRRDLMYEGFSSIPGFKLNKPQGAFYIFPEISELLGK
                     QYNGETIKTSDDLAMFLLNHAHVAVVPGSAFGSPKCIRLSYATSDDKLKESIERIRKA
                     VALLK"
     gene            3757..4272
                     /locus_tag="PK049_05090"
     CDS             3757..4272
                     /locus_tag="PK049_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60964.1"
                     /translation="MFAEILKITIPALMVFLAGYLALRTLIKNETQRQKAELMFNTIK
                     VSLPLRLQAYERLILLMERISPESIIVRVNKKGMLASDLQSALLGNIRAEWEHNLSQQ
                     LYVSNEAWEMVKNAKENIVKLINICSEKVAPNDSSFDLSKKILETYAELEINPTSLAI
                     EFLKKEVKEIL"
     gene            complement(4281..5105)
                     /locus_tag="PK049_05095"
     CDS             complement(4281..5105)
                     /locus_tag="PK049_05095"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOK60965.1"
                     /translation="MIKLVVTDLDGTLLRSDHKFNGEDMVSLCRLGEMGIIRAIATGR
                     SPFSASTVLPDDFPIDYLLFSSGAGIMRWSDKSIIHANQLSPEVVQYVIALLVEMHAD
                     FMVHEPIPLNHCFHYHSSGKLNSDFERRISLYKDYSQPLITGVPFPGPASQVLAVLPN
                     DIERFNAISKKLNGVQVIRATSPLDGKSIWLEIFPEGVSKSGGIQWLCSYIGGIQPRN
                     LLALGNDYNDIDMLNLAGEAFVVSNAPSELKDKFQVVPVNDEAGFSHAVKRVLKID"
     gene            5425..5685
                     /locus_tag="PK049_05100"
     CDS             5425..5685
                     /locus_tag="PK049_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008153583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOK60966.1"
                     /translation="MTKIITFNELRRVKDSLPDGSMQVIADRLNVNVETVRNYFGGTN
                     YKDGGAVGFHLEPGPDGGIVTLDDTTILDIAYEIIAKSKEMA"
     gene            5766..6623
                     /locus_tag="PK049_05105"
     CDS             5766..6623
                     /locus_tag="PK049_05105"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOK60967.1"
                     /translation="MEELLSKIHRIETKHLSNLHRHLESIFTNVSLPSHNLQHHIRVW
                     LHCRGLLIELHKAGLTITEQLIENAIIACFFHDAGLTKTLDEQHGAEGAELCKQYLSH
                     FPDLTESQKTLILKAVELHDDKSVKQHPVTKPDDMLDLALLVSTADDLDALGFVGVFR
                     YTEIYLKRGISIDQLPRKVLANLRNRFTSFTNAYSSLHRYSDRQRQRYREIMDFFTKL
                     EGEISQGITYQDSAYAVVNTLHEQLVIQSNSIEQTIDYALSTLTASYPLTFFKRLQLE
                     LEVTSAIPI"
     gene            complement(6663..7274)
                     /locus_tag="PK049_05110"
     CDS             complement(6663..7274)
                     /locus_tag="PK049_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOK60968.1"
                     /translation="MKIICIGRNYRDHAKEMNADVPEVPVFFIKPDNCLLRNNQPFFY
                     PDFTSDLQYELEVVLRINRLGRSIKQKFAHRYYDAITLGIDFTARDLQRECKAKGLPW
                     EISKAFDYSAPIGTFIPKDELGDLNGLNFRLELNGKVVQQGNTANMIFGYDELISYVS
                     RFITFRTGDLLFTGTPSGVGPVNVGDRLQAYLNDRLMLDFFIK"
     gene            7379..8281
                     /locus_tag="PK049_05115"
     CDS             7379..8281
                     /locus_tag="PK049_05115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HOK60969.1"
                     /translation="MADPITKSTRKRILFIVNPISGTRGKESILHIINNTIDNETFEP
                     EILFTRKKGDALQIISEKIAEGFKYFVAVGGDGTVNEVGSAIINTDCVMGIIPLGSGN
                     GLARHLRIPLDAAKAIQVINKLRVESIDYGLVNGLPFFCTCGVGFDAHIGYRFAKEKG
                     RGFFNYVKVALTDFIRYKPKKYKVKIDGTQKMKVKAFLITCANASQYGNNAYIAPNAD
                     IQDGLLDIAIMSPINLLQAPAIGAMLFTRTINKSTVLQTGKAQKIVIKRKREDVVHFD
                     GEPIIMDRKIRITVVNKGLRIIVP"
     gene            complement(8278..9021)
                     /locus_tag="PK049_05120"
     CDS             complement(8278..9021)
                     /locus_tag="PK049_05120"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOK60970.1"
                     /translation="MKHSVAKATFFLGIAAIYRCFLILAGIGYYVYLLVTKQYSYNAF
                     YFFACFLVSFIGLVIRAITIGYTPKNTSGRNTKEQVADTVNTSGMYSLVRHSLYLGNF
                     FMWFGLALLTKSYWFVISFLLVYWLYYERIMYAEEFFLRNKFGSDYLNWADSVPTFIP
                     RRFRWRSPNNYFSIRNVIKRESIGLFKLVLLFFVFQYIHDVVSNGGKLLELSSWGRVW
                     FIAFIIVGVIYLTLRTIRKKTRLLDVDGR"
     gene            complement(9101..9868)
                     /locus_tag="PK049_05125"
     CDS             complement(9101..9868)
                     /locus_tag="PK049_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOK60971.1"
                     /translation="MGLNIKNPLVVFDLETTGIDVVKDRIVEIAVLKVFPNGNRESKV
                     RRINPEMPIPPEATAVHGITDDDVKDAPTFKEIAKSLANYIEGCDFAGFNSNKFDLPL
                     LAEEFLRAGVDIDLKKRKFIDVQTIFHKMEKRTLAAAYKFYLDKDLVNAHSAEADTLA
                     TFEILCAQVERYPELKNDVDFLSEFSSFNRNVDFAGRIILDDKGVEVFNFGKHKGKPV
                     VEVLKAEPSYYSWMMNGEFPLYTKKVLTNIYLKMKQK"
     gene            complement(9936..11066)
                     /gene="dnaN"
                     /locus_tag="PK049_05130"
     CDS             complement(9936..11066)
                     /gene="dnaN"
                     /locus_tag="PK049_05130"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOK60972.1"
                     /translation="MKFVVSSTMLLSHLSVVSRVISSKNTLPILDNFLFKLEGNELEI
                     TASDLESTLTTKITLDNVLGDGSIAIPFKILIDTLKEFSEQPLTFEIDPGSLAVVIHS
                     ENGQFNIVGQPADDFPKPAELSPESKVSVKVAADVMLNGITKTIFATADDEMRPVMNG
                     VFVELTTEGMTFVASDSHKLVRYRRKDVQVEEFSSFILPKKPANLLKSILVKETGSVN
                     VEFDSKNARFTMSGYTLVCRLVEGEYPNYSAVIPVDNPNRMIIDRVDFYNSVRRVSIY
                     SNAASNLIKLGLTSNQCEVSAQDLDFSVSAYERLNCDYQGDEMEIGFKSAFLVEILAN
                     LSSTEVVLSMSDPSRAGLIFPHQNENPDEDVLMLLMPMMIGA"
     gene            complement(11221..12192)
                     /locus_tag="PK049_05135"
     CDS             complement(11221..12192)
                     /locus_tag="PK049_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60973.1"
                     /translation="MSSKKIIFLSLLIALGIGLFYLSRWYKEFRQSDFSFSSSQITAI
                     ELVMKSDTLRLTKSDGNWFINDFFLADSAVVSNFIDILQSISAIAPATLKTDENLIQL
                     FQRKGIGVFIYKGKRVKKEYRIASVAEFNYKTVALNSGSEVPYYVESANAVDNLVEYF
                     SVNPDKWLAGKLFPEPVEAIEEIKAIFPDKAKGYTIELGGNQIALINSAGDRLSNINL
                     ANISNLYYSFDNLKLLYPTKAETNSAIPENIISSLKLKMLNGKTYEYTFYRIAGKGFT
                     NILGQELSYNPNRLLVKLSENRFLVGSYLHFHYVLCPIDVFVNNYEK"
     gene            complement(12182..13900)
                     /gene="gldG"
                     /locus_tag="PK049_05140"
     CDS             complement(12182..13900)
                     /gene="gldG"
                     /locus_tag="PK049_05140"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HOK60974.1"
                     /translation="MSVSGKIKIRSIRAFAVLLLILLLVNALSSFFYFRIDLTEDKRF
                     TIQPVSREVLQGLDDIVVIRVYLDGELPVAFVRMKKALAETLSEFQEIAGKKIRYEFV
                     NPYSTSGAKKQEQLFRQLAEKGLVPTNVHVKDNEGAMIQRIIFPGCIITYRGKELAVN
                     LLRNNPNLTGDENINLSIQNFEYALVDAIDRITTEHRQRIAFIQGHGEFDEFETGDIE
                     RELAQYYDVSRVIINGDTDALKPYKTIIIAGPSRPLPEADKLIIDQFIMQGGRVLWFV
                     DPVNVSIDSLSSGASTLAFMNQHNLDDMLFRYGVRLNPLLIQDIQCAVIPVNTALVGQ
                     EPRFVPAPWLYYPLLLPPADNPITKGLNLIQTKFISPIDTVGMDDEVEKRILLFTSKE
                     AKLVKIPALINLNQVSKGITRYEFQHSMLPVAVELQGIFKSNFRNRPINAIIPNGTIK
                     FHDKSIFTKMIVVADADIIRNDIQRRPNGAYVIPLGYDRFTQQTYGNKELVVNMVKYL
                     CDDSGLLDLRSRDVKLRLLNRKRAVSERLYWQLVNMVLPSLMLVLAGIIWSFVRRKKY
                     TFTNVK"
     gene            complement(13920..14651)
                     /gene="gldF"
                     /locus_tag="PK049_05145"
     CDS             complement(13920..14651)
                     /gene="gldF"
                     /locus_tag="PK049_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     permease subunit GldF"
                     /protein_id="HOK60975.1"
                     /translation="MKAIFIRELQNFFSSLTGYVIAIIFLVLTGLFLWVLPGYTNILD
                     SGYATLESLFYIAPWLFLFLVPAATMRMFADEKKTGTIELILTRPISDTAMVLGKFFA
                     SLVLVAIVIVPTFIFVYSVSNLGNPSGNLDWAATWGSYIGLFFLASAYAALGVFASSL
                     TDNMIVAFLLAVALCFVFYDGLAWVAELQSMEWARSALLTLSISEHYSSVSRGVIDSR
                     DVVYFLSISSVFLALTRLKLQSRRW"
     gene            complement(14676..15860)
                     /locus_tag="PK049_05150"
     CDS             complement(14676..15860)
                     /locus_tag="PK049_05150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOK60976.1"
                     /translation="MRVVLYSFLLFLPFISNSQKLSISVFNDTNLNTILVTPTKGEYS
                     MVLGDSIITIKPLQILYISRQGDSVLVRDENQSYGSFKRVTLIGQTGDDVFRLKPIIP
                     AKPARLYDDNITFYVEFNRIMAVNIIDREKYIAAVVLAETGPNKEPELYKAQALLART
                     FTVTHIDKHQSEGFNLCDQEHCQAYKGRPEAFQEIYDAVLDVKGLIVVDSSLKPISAA
                     FHANCGGQTANSEDVWIQALPYLRSVNDRYCTSAPNARWERTIPLTEWKKFLAQQGVD
                     TAKVTRDNLNFYPKERPKYYTVLGVNIATTAVRKYFKLRSAWFTVSAGKSEVRLSGKG
                     YGHGVGICQDGALAMAQRGFTYDKIIQFYFKDIQIIDISKVNLDSLKPDTLEVKPFDV
                     LQ"
     gene            complement(15871..16995)
                     /gene="lpxB"
                     /locus_tag="PK049_05155"
     CDS             complement(15871..16995)
                     /gene="lpxB"
                     /locus_tag="PK049_05155"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOK60977.1"
                     /translation="MKYFIVAGEASGDLHASNLMKGIKNNDPDADFCFFGGDLMAEQG
                     GTLLKHYRDMAFMGFLEVLLNLRTIKANMELCKQSIIHFRPDVVILVDYPGFNLRIAK
                     FAKLSGFKVFYYIAPKVWAWKESRVEKLKSFVDKLFVIFPFEIDYFRGKGIDPVYEGN
                     PSIDAIANKLPNPISLSDFCEKNGLPQKPIIALVAGSRKQEIRYNLPVMLNVTKRFPE
                     YQFVIAGAPSLDYSVYQPYISGTGVSVVFGQTYQLLSCSTLAFVTSGTATLEAALLNV
                     PQVVCYRGNFFSMLIAWMVIKVKYISLVNLNMGFEVVRELKQYDLTPDSLYTEAIKLL
                     PGNPERDRQLELYGQLKEKLGKMGASERVGKRIVDLIKSS"
     gene            complement(17002..17292)
                     /locus_tag="PK049_05160"
     CDS             complement(17002..17292)
                     /locus_tag="PK049_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60978.1"
                     /translation="MQKKISKQWHGYLLGALLPVITLVVMYLSLASDFKFVDFIRYLN
                     VRGDLSRALSLAVLPNLGLFFVFIWTDRLKAAQGVLGSTIILALIIVFMRFM"
     gene            complement(17273..18100)
                     /gene="surE"
                     /locus_tag="PK049_05165"
     CDS             complement(17273..18100)
                     /gene="surE"
                     /locus_tag="PK049_05165"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009235142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOK60979.1"
                     /translation="MFEGNGELKRPTILVTNDDGFRAAGIEALVEMVKPFGNVVVVAP
                     EEGHSGMSHAITIKTPLRLKKRKRTDDVEFYSVNGTPVDCVKLAMNQIFTSKLPDLVV
                     SGINHGSNSSISIIYSGTMAAAIEGCLYGVPSIGFSILDYDENPDFTAAIEYGRFIVK
                     NVLENGLTPGSCLNVNVPVLPLKSVKGVKVTRQNKGTWREEFEKRTDPRGVDYYWLTG
                     YFLNEEPNATDTDEYWLNQGYITVVPVHVDLTDYKELDRIKSWRFDQIAISHAEKNK"
     gene            complement(18303..18557)
                     /locus_tag="PK049_05170"
     CDS             complement(18303..18557)
                     /locus_tag="PK049_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60980.1"
                     /translation="MENNHTILGIKITDRIKEAGEVQRVLTRYGCSIKTRLGLHEVSD
                     DYCSSSGLIILELTGPADEQAKLHDELVKIPGVITRRMDF"
     gene            18709..21747
                     /locus_tag="PK049_05175"
     CDS             18709..21747
                     /locus_tag="PK049_05175"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic secretory protein-like protein"
                     /protein_id="HOK60981.1"
                     /translation="MNRSYSISILLALTFISQIVYGQYFGRNKPIYKNFKFTVYQTPN
                     FEVYHYLKNDSLLNIISLSAEKWYDRHYQVFRDSIITKNPILLYNDHADFQQTTAISG
                     SVGVGTGGVTEALKNRVIFPLTETWQQTDHVLGHELVHAFQYNSLINGDSTSLNSVRN
                     LPLWMVEGMAEYLSLGTYDYQTAMWMRDAVINDRFPTLEDMTYYPNKYFPYRWGHAFW
                     SFIGRTFGDSLINPIFKETAKRGYNFALMRYLGLNEQSFSTLWKSVYYDYFKRTMPDT
                     VEDPNGTRILFERNAGEINISPSVSPDGRYVAFYSEKDLFSIDLYLAHAISGKIIRRL
                     SSIVHDNEIDALNFIESAGTWSPDSRMFAFVGFSKGKSKLIIVDVFDGKLIQEFTIPG
                     VPSFNYPAWSPDGSSIVVSGLVNGVNDLYQYDLRIKKVDRLTNDPWCNIHPAWSSDGK
                     TIVFSTDKPSQTSANGKTGYNLATLDVETKTINVFDILPGADNVNPMFSANNKEIYFL
                     SNCDGYRNLFRFNIESGEVYRMTRILTGISGMTPLAPAMSVARENDEVVYSHFFKSKY
                     SIYTADSSDFKPVRINPYQVDFNASILPPVNRATEGLVDKNLSDKNLPVVLVDSFKTV
                     PFKPKFKLDYISNIGMGISTSPYGTGMAGGVEMLFSDITGKNMVYTGLSLNGEIYDFG
                     GQVAFLQQKKYITWGVTASHIPYSFGQYGYDTLHRQINGEPVIVEDFQLIQIRMFETA
                     TGLLSYFPLSSTQRFELSGSIARYYYRVDIYSNYYIDGYYYGSERKKGEVPPGFWLQQ
                     SSLAYVYDNSDFGIASPMRGHRMRFQASRTLGEYNYWGALADMRKYIFRKPLSFAFRA
                     LYYGRFGTSINDNILYPLYIGYPWYVRGYDSNSFINYGQSGNVTIDQLTGNHIAVANF
                     EIRVPFTGPERLTLIKSGTLFTELAFFTDAGIAWTKEYPPSLKWKPTSINDRTPFVSS
                     GISLRINLFGMLILEPYYAIPYQLGGFKAANWGINFIPGW"
     gene            complement(21750..23078)
                     /locus_tag="PK049_05180"
     CDS             complement(21750..23078)
                     /locus_tag="PK049_05180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOK60982.1"
                     /translation="MKLDISYREIWRVAYPIILGSVAQNILNITDTAFLGRVGEIALG
                     GGVLGGLLYHVLLMLGWGMAMGGQIIVARRVGEGANEAVGRVVEHLLFTLGAFAILIM
                     LFAHALHPAFFSIAVRSNLVCDSASSFFGYRLFGLPFAFLTYSLNAFLIGITRTKVIT
                     LTSFITVFVNIVLDYGLIFGNFGLPQMGVRGAALASVIAEIAGATFLLVYISRMNVSK
                     YKLFNFSSFSKKLLKSILDISWPLMLQFSLSIFVWFVFFLIIEKMGETPLAVSNIVRS
                     IYIILMIPIWGFASATNSFVSQHIGKGKSDQVVKLVVKVMVITLASVLVLSALVLLLG
                     DSIIKIYTNDEHIINATLPVIRVIAIAALTMASAFISFNGVSGTGSTKVSFAIEVFTL
                     TFYLGWAYLIAIILAKSLSLVWTSEVLYGILVTVISIIYLRTGRWKNRVV"
     gene            complement(23145..25271)
                     /locus_tag="PK049_05185"
     CDS             complement(23145..25271)
                     /locus_tag="PK049_05185"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOK60983.1"
                     /translation="MKRFFSTFLIITLVFTAFGQKELTLEQAVLRQGTEFRPKTLPGF
                     SWLPGTDEYSFVSNDSLIIVNVTDSKKNRAVTLSEVNEALKANSLQPLKRVPSAWGDG
                     KQLRFIAGNYFVLYNSVTKSIASKFLVADNSENHEFSPGGRWMSYTIGNNLFLTNAQG
                     EVITVTNDTTDGIVNGQSVHRNEFGINKGTFWSPSGRYLAYYHMDESMVTKYPLVDIS
                     KRVGELVNIRYPMAGMQSHVVTVRVYDTQSGSTVELKTGEPRDQYLTNIAWTPDERSI
                     LIAVLNREQNHMCMNRYNVSTGDFELTLFEESSSKYVEPLEPPFFINSNSFLWLSRRD
                     GWNHIYLYLADGKFVKQLTKGEWEVTSIDATDFKNGLIYFTSTKESPLDRHLYSVSTK
                     NGSITKLTTSSGTHRCMISPSYKYFVDFYSSITVPNRVALHTIKGKLVKQLIDAPNPY
                     TGYNVSLPNLITLTATDGTTLYGRMIKPVNFDSTQKYPVVIYVYGGPHSQLVTNSWMG
                     GARLWECYMANKGYILFTLDNRGTSNRGAQFEQAIHRQLGTVELGDQLVGVNYLKSLP
                     YVDSTRLGVHGWSFGGFMTITMMLRASDHFKVGVAGGPVTDWKFYEVMYGERYMDTPD
                     ENPDGYKKADLKNYVTNLKGKLLIIHDDMDKTVVPQHSLTLIHEFIKNGVQVDFFMYP
                     QHDHNVMGKDRVHLIDKIIRYFDDNL"
     gene            25448..26356
                     /locus_tag="PK049_05190"
     CDS             25448..26356
                     /locus_tag="PK049_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60984.1"
                     /translation="MIETLTMSLRLPKSKLALVLISAFLASCKPSPKQDNETTQPAAD
                     TLSTLVKVENAIFPIPSPWQVVNLIQKNNIPFNESCINSTKNYQRYTTSFKQAVNLGI
                     YGTDLSYLNIYDKTQESLQYLSVIKKLSEQLRITESFDEKIFERIENNIENKDSLIKI
                     ISDTYASSDLYLKKNERYDISALVVAGCWVESLFIMTKIAQEHNDREIINRIGEQKHP
                     LDNLVELLTPFYYKSTEFANLLDSLIDLAYEFDGIIYSYTYREPIVDEANRVITIRSQ
                     SRVVMSEYHLKIISKKIEQIRSSLTE"
     gene            26367..26777
                     /locus_tag="PK049_05195"
     CDS             26367..26777
                     /locus_tag="PK049_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60985.1"
                     /translation="MQRIVIILTLTLFSLNSFSQIEQLKKTCSINFPSNYISDGQEYF
                     AALKPDQRIEFRTTFYADNTYRISACTNAKKGKLVFTVYDTEKNLLFTNESFNYSPFW
                     DFRFTSTVTCIIQVDVKNPQFIPGYVMLLIGFKQ"
     gene            26791..29892
                     /locus_tag="PK049_05200"
     CDS             26791..29892
                     /locus_tag="PK049_05200"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOK60986.1"
                     /translation="MSESILKALMQLFAIIARPVSLDEKENNDYADRRKVVEEFLRNQ
                     LNIDLVKEYLKVFDYYYNLYQEKQSEKSKSKKRTSSSSVRVLKICSQINEELQQQQKI
                     VVLIRLLEFVKPDEGEVTEQEMAFISTVAETFYVPEDEFQNLKDFVLSGFDSVIPHQN
                     ILLINDSKEPPYDDLRHLYAEGLHEPIKILFVESANIFFLRYNGLHELYLNGHLLVKD
                     KIFVLNNGSSIRNSKIKPIYFSDIVGRFVQDKIKEKIVFEAIDIEYRFKSGKIGLHPM
                     SFVQESGHLVGIMGASGAGKTTLLNVLNGSEKPTKGSILINGIDIHNQKELVEGQIGF
                     VSQDDLLIEELTVYQNLYFNARLCFDNYTHEQLEAAVNQTLQNLGLYEIKDMRVGSPL
                     NKKISGGQRKRLNIALELIREPAILFLDEPTSGLSSRDSENILDLLKELTLKGKLVFV
                     VIHQPSSEIFKMFDRLLILDTGGYLIYNGDPIESIIYFKSRVQQANWNESECPVCGNV
                     NPEQIFNIVESNVLDEYGNPTRARKISPFEWNEYFKRFAPENPTPNTQPKSLPQIHFK
                     IPNWVKQLRIFVKRDILSKLANSQYMIINIFETPLLALLLAYIIKYYNVDVSNKLGYT
                     LLENSNLPVYLFMSVIVGIFVGLTVSAEEIIKDRKILKREAFLNLSWSSYLMSKVTIL
                     LGISAFQALMFVTIGNSIMEIKGMYFEYWLVLFSTWFSANMMGLVISDSFKTVVTIYI
                     LIPFLVIPQIILSGIIVKYEKLNPTISSPSKIPIYGELIVSRWAYEALAVYQFKENRY
                     MKPLYRFDEAMSLSDYKRNYWLKSIQNKVDFCIRNLNDKEKKAEFKNTLELIRNELIK
                     EMTAPRASHLIFSKYTMLTPEEVNADILKELNDFLEQLRKHYVKLYNKASAEKDNYIT
                     RFQNSQPNGQQLFLELKRRYHNESLSEFVRNSGEVERIIEYKNQLIQKIDPIYQFPES
                     KFLRAHFYAPVKPFFGNYFGTIWVNVIVIWLSSILMYLVLYYRLLKRLLEWLESLGGE
                     TES"
     gene            complement(29955..30335)
                     /locus_tag="PK049_05205"
     CDS             complement(29955..30335)
                     /locus_tag="PK049_05205"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOK60987.1"
                     /translation="MELLNIEGTPKTPTVILNPSTGVIEIKGRSIPENSIEFYRPVVE
                     WLDEYIKNPQPNTVVNVQLEYFNTSSSKCILDVFKKLEVLKKNQKDVIINWYYEEDDE
                     DMLEAGEDYESIIKVPFKMIQIVD"
     gene            complement(30352..30894)
                     /locus_tag="PK049_05210"
     CDS             complement(30352..30894)
                     /locus_tag="PK049_05210"
                     /inference="COORDINATES: protein motif:HMM:NF038262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HOK60988.1"
                     /translation="MSDFISKCYAKMHSGDVIVAYKGEINANIIADTLGLVEAKLSHE
                     MVENLVRKKLYNVMVECLQNLFHHVDKVTLSENEPRMGAFILSKTNDGYRIATGNLVR
                     NDKSNILKEKIDKINSLSKDELKEFYKFVLNNQTFSEKGGGGLGLIDIARKTGNKLNY
                     EFTPYNDEYSFFNLSVLVSE"
BASE COUNT         9587 a         6621 c         6086 g         8683 t
ORIGIN      
        1 ttgccattac tacccttcgc ccatcgggta aaatccaaat aggaaatagt tggtttgatg
       61 ccgttgccga atttggctac atcgaaaagg ggagcaaggt taaggtatca aaggacgagt
      121 cggggcaact ttacgttgtt aaagcataga attatggaaa gtattaccat tcgagacttt
      181 cagcctagcg attatgaagg gttaagtgag ctttggctgc tcaccaacct tggcaatcct
      241 gctcgtggcg acaacctgga aataattcag caaagcattg acctgggagg taagctgctt
      301 gtggctgtaa attcggatgg aatggtgatt ggtacaagct ggatgacttt tgatggccga
      361 agaattcact tacaccattt tggcgtccat cctaactacc aaaggcaggg gataggaaaa
      421 atgcttaccc gcgaatcgct taggtttgtc aaagccaaag gctatcaggt aaagctcgag
      481 gtacatcgca ataatattgc agccattaac ctatacaaac agttcggctt tcaatacctt
      541 ggcgattacg atgtatacat tatcagggat ttgaatagca taagcattta aaagccaaca
      601 cattcctata gaggaacaaa aaatgcatcg aattttttcg atgcattttt tatcttaaaa
      661 aaacctatta tcaaaaaata cctttaccca ttagcgactt actggattgt tcatcattgg
      721 tcgttgagtt tccctgagct taagtatctc cttaagtttt tcggtttcca tccaaaccct
      781 tgggcgagtt tttaggccaa caccggcaca gaacaggtta atgttctgtg agacaattgc
      841 tgcatccatg gcagcccaag tcattttttg gctctcatcg cctcgctgaa agcgggcaac
      901 atcggagaca atcacaagaa aaataggtgc tttggcaaag ttttgctgcg aaccggcaac
      961 tgctgaacgg taatcaccac tactgattag aactagttca tgagccttgg catcgtacaa
     1021 aaatgctccc ttgctattta ccaggtaaac atcaatatcc tgagtgttca tggctgttgg
     1081 ggcagttcgt tttccttcgg caggacggtt aataccgttt gcagcccaca gcaaatcggc
     1141 tatttcctga aggtctaatt cctgagtatc aaattcccta accgaggcac ggttagcaaa
     1201 ggcttccatg atgtttttac ccttaaccct gctgggttcg ggtagtttgg taatgctatc
     1261 gttttgtccg tttgatacca acgataacaa aactgccact aagaatggta acgttttcat
     1321 atcaaatatt tattaacgat aaattcgttc catgttaagc gcccttcggt attccgctgc
     1381 gtttctatta tgttctgcca atgtttgggc aaaaatatga cgtccagaaa aatcgggcga
     1441 agcgcagaag taaagatagc tatgtttttg cgcgtttagc acagcatcaa tagcaataac
     1501 tgaaggtatc cggatagggc caggaggtaa tcccttgtgt ttataggtat taaaaggaga
     1561 gtcaacggct aaatggcggg ttaataccct tcggattgta aaatcgccaa tggcaaactt
     1621 aaccgttggg tcagcctgga ggggtattcc cttccgtaat cggtttaaat aaaccccagc
     1681 aattattggc atctccgatt taacattggt ttcttcctca acaattgatg ccaaaattgc
     1741 tacctcaacc ggcgataatc caattccatc ggctttatga atccggctct cgttccaaaa
     1801 acggttatat tccttataca tcctattgat aaatcctaat gcatgggtat tccagtagaa
     1861 ctcgtatgta ttaggaataa acattgccat aaaagtgcta ggagtgaaac cccgattaaa
     1921 cgctactgaa tcggaagtaa acagattaat aagcgaaacc gaatcggctt caatctgtga
     1981 agaaatgcgt tgtgctagct gttgccaggt tcgcatgcca gtaaaggtaa cctttactgg
     2041 tttttgatgt ccatacttaa gcattttaac catggaggaa ttactcatac cgggcttaaa
     2101 agcatagcat ccgggtttaa cagatttttc gtaatcgtag tattgtgcca cccttttgaa
     2161 cctgtcgata ctttttaata ccttgttttt atccaggata tttagaacat cgttatagtt
     2221 tgaacctgtg ggaatatata ttaatgtctc ctttgagact gctacattgg tagcacgaaa
     2281 cgtaaaatag agccttaaaa gaagcaaaac tccaaccgct aatccaacca aggcgataac
     2341 caacccaaac tttacaaatt tcttcatctt caaaaaagta atactaaact aaaataaaaa
     2401 aaagcatcga aataaccatg tagttcgatg ctatataatg ttaaatggaa atagctttac
     2461 ttgagtaaag caactgcttt cctaatacgc tcaatggatt ccttaagctt atcgtccgat
     2521 gtagcatacg aaagccttat gcattttggt gaaccaaaag ccgaacccgg aacaacggca
     2581 acatgtgcat gattcagaag gaacatggca aggtcatcgg aggtttttat ggtttcaccg
     2641 ttatactgct tgccaagcag ttccgatatt tcagggaaaa tgtagaacgc accctgtggc
     2701 ttattcaact tgaagccagg gattgatgag aaaccttcat acatcaaatc gcgacgacgc
     2761 cgaaaagcct cgcgcatttg gcgggtgtat tcgccatcgg agttaaatgc agcaatggct
     2821 gcacgttgag caatactgca aacgcccgat gtttgctgac cctgaagttt ctcacatgct
     2881 ttggcaatcc atttggccga tgccgaaaat cctatgcgcc aaccggtcat ggcatacccc
     2941 tttgaaacgc cattgataat aactactcta tcccgaatag aatcaaactg agcaatactc
     3001 tcatgtttgc caataaaatt gatatgttca taaatctcat cggaaattac aatgatattt
     3061 gggtgcttct caataatttg agcaattgcc tttaattcat cgtaggtgta tacactaccc
     3121 gtagggttag aaggcgaaca taaaagtagg gctctggttt taggtgtaat agcagcctca
     3181 agctgtttgg gggtaatctt aaaatcgctc tcaagcgagg tttcaatcac cacgcttttc
     3241 ccctcagcaa gcttaaccag ctcaacatag ctaacccagt atggggcagg aacaataacc
     3301 tcatcgtcct tatcaacaat acacatcaac acgtttgaaa ggctatgctt agcacctccc
     3361 gaaaccacaa tttggtcggg ctggtactcg aggttgtttt cgcgtttaag ctttccacaa
     3421 atagcttcca gtaaatcctt gtaacccgca accggtgtat aaaacgaaaa gttctggtcg
     3481 atggctttct tggcagcatc ctttacaaag tcgggggtaa agaaatcggg ttcgcctacg
     3541 ctaaggctaa ttacatcgat accctgtgca gcaagttcgc gcgacttttt cgacattgca
     3601 atggtttgcg actcctcaag agctgctatt ctggctgata ccttttccat ggtttaacgt
     3661 tttagggaat tccaaattgt tttactaatt ttagagtttc aaaaataaag taatttgtca
     3721 ttcatataca cctaaactgt ccaaaaacat taaaatatgt ttgccgaaat tctgaaaatc
     3781 acaatacccg cacttatggt attcctggca ggataccttg ccttgcgaac tcttatcaag
     3841 aatgaaaccc aaaggcaaaa ggcggaatta atgtttaaca ccataaaggt ttcactaccg
     3901 cttcgacttc aggcatacga gcgcttaata ctcttaatgg agcggatttc gcctgaatca
     3961 ataattgtaa gagttaataa aaaagggatg cttgctagcg acctacaaag tgcgttactt
     4021 ggaaacatca gagcggaatg ggagcacaac ctttcccagc agctatatgt tagcaatgag
     4081 gcatgggaga tggttaaaaa tgctaaggaa aacattgtta agctaataaa tatttgcagc
     4141 gaaaaagttg caccaaacga tagcagcttc gatttgagca aaaaaattct tgaaacttac
     4201 gctgaacttg agattaatcc cacctcgctg gccattgaat ttttaaagaa agaggtaaaa
     4261 gaaatacttt aaaaccttgt ttaatcaatt tttagcaccc ttttaacagc atgtgaaaaa
     4321 ccggcttcat cgtttaccgg cacaacctga aatttgtcct ttagctcact gggtgcgttt
     4381 gaaaccacaa aagcttcacc ggcaaggtta agcatatcaa tatcgttgta atcgttgcct
     4441 aaggccaaca aattacgggg ctgaattccg ccaatatagc tacacaacca ttgaatacct
     4501 ccggattttg aaaccccttc gggaaaaatt tcaagccaaa ttgacttgcc atcgagcggg
     4561 gaggttgctc ttataacctg aaccccgttt aactttttgg aaatggcatt gaacctttca
     4621 atatcattgg gtaaaacggc caacacctgc gaagcaggtc ctggaaatgg cacaccagtt
     4681 attaagggtt gagagtaatc cttatagagg ctaattcttc gttcaaaatc agagtttagt
     4741 ttaccagaac tgtggtagtg gaaacaatga tttaagggaa ttggctcatg aaccataaaa
     4801 tcggcatgca tttccacaag cagggcaatt acatactgaa caacttccgg tgaaagctgg
     4861 ttggcatgaa taatcgactt atcggaccag cgcataattc ctgctccaga agagaacaaa
     4921 aggtaatcga tgggaaaatc gtcgggcaaa acagttgatg ctgaaaatgg tgaacgacca
     4981 gtagcaatgg cacgtatgat ccccatttca cctaatcggc atagggaaac catatcttcg
     5041 ccgttaaact tatgatcgga acgcaggagt gtgccatcca aatcggttac caccagttta
     5101 atcatgaaaa gcctatattt tccgcaaaga tattaaggtt cctggaaaag tttaaagcca
     5161 ctatggtata tgtggtttaa aagtaattta gagtttaatg atttatttgt aaaacaatga
     5221 ttgtctttgt gttaacctcg aatcatttac cagaatagta atattttcca ctacttcaat
     5281 attaaaacat ccaaaaggaa atttcgtata aaaaactaga aaataatgtg tttttttaac
     5341 gttaaaattt ggttaaatca ttttacattt tgaacttaat tttttatatt taaggtgtaa
     5401 aaatcatttt aaaaccctgt aattatgact aaaataatca catttaatga gctcaggagg
     5461 gttaaagata gccttcctga tggtagcatg caggtaattg cggacaggtt gaacgttaat
     5521 gttgaaactg tccgcaatta ttttggtgga accaactaca aggatggagg tgctgtggga
     5581 tttcaccttg aaccgggacc tgatggtgga atagtaaccc tcgacgatac cacaatactc
     5641 gatatcgcct atgaaattat tgccaaatca aaggagatgg cttaaaacaa ttctatccaa
     5701 atcacgtaaa agcaggaaaa tttttcctgc ttttttattt actttgcata aactacatac
     5761 aaaccatgga agagttactc tctaaaattc ataggattga gacaaaacac ttgtcgaacc
     5821 ttcaccgcca ccttgagagt atattcacaa atgtgtcgtt accctcgcac aatctgcaac
     5881 accatattag ggtgtggttg cattgccggg gtttactgat tgaactgcat aaggctggac
     5941 taacaatcac tgaacagcta attgaaaatg caataattgc ctgtttcttt cacgatgctg
     6001 gcctaaccaa aaccctcgat gagcaacatg gggctgaggg tgccgagcta tgcaagcaat
     6061 atctcagcca tttcccagac ctaacggaat cgcaaaaaac actgatttta aaagctgtag
     6121 agttgcacga tgacaaatcg gtcaagcagc acccggttac aaagcccgat gacatgctcg
     6181 acttggcttt actcgtatcc accgccgatg atcttgacgc acttggattt gttggcgttt
     6241 tccggtatac tgaaatttac ctgaagcggg gcattagcat cgaccagctt ccacgcaaag
     6301 ttctggcaaa cctacgtaat aggtttacaa gttttacaaa tgcatactca tcgctgcacc
     6361 gttattccga caggcaaagg caacgctacc gggagatcat ggactttttc acaaaacttg
     6421 aaggtgaaat atcgcaaggc attacctacc aggatagcgc atacgctgta gtaaatacct
     6481 tgcatgaaca gctggtgatt caatcgaaca gcattgaaca aaccattgac tacgcattaa
     6541 gcacactaac cgctagttac ccgttaacct ttttcaaaag gttacagtta gaacttgagg
     6601 ttacctcagc tatacctatt taaggttaaa gggaatacga caatagtttt gctgtaaaac
     6661 cattacttaa taaaaaagtc gagcatcagc ctgtcgttca aataggcttg tagcctatcg
     6721 ccaacgttaa caggccctac accgcttggg gtgccggtaa aaagaagatc gccagttctg
     6781 aaggttatga atcgtgaaac gtacgaaata agctcatcgt agccaaaaat catgtttgca
     6841 gtattccctt gctgtactac ctttccgttt agctcaagcc gaaagtttaa gccatttaaa
     6901 tcgcccagtt catctttggg gataaaagtt cctattggag cagaatagtc gaacgctttg
     6961 gatatttccc agggtaatcc ttttgcttta cactcgcgct gtaagtcacg agcagtgaaa
     7021 tcgataccaa gggtgatggc atcgtagtaa cggtgggcaa acttttgctt gatactccgc
     7081 cctaacctat taattcgaag cacaacttct agttcatatt gcaaatcgct tgtaaaatca
     7141 ggataaaaaa aaggttgatt attgcgaagc aaacaattat cgggcttaat aaagaatacc
     7201 ggaacctcag gcacatcggc attcatttct ttagcatgat ccctatagtt tctaccaatg
     7261 caaataattt tcattatgag taatttttta tgtcaatgaa tgagcaaagc caaacaaagg
     7321 tagttagttt gatgctatta aaaaaggttt tacttttgca tgttcaaaag taagttccat
     7381 ggctgatccc ataacaaagt ccacccgcaa aagaatactt tttattgtaa atcccatttc
     7441 tggaacacgc ggtaaggaaa gtatactgca cattatcaat aatacaattg acaacgagac
     7501 atttgagccc gaaatccttt tcacccgaaa aaagggtgat gctttacaga ttataagtga
     7561 aaaaattgct gaggggttca agtattttgt tgcagttggt ggcgacggaa cggttaacga
     7621 ggttggcagc gccattataa acacggattg tgtaatgggt attatccctt taggttcagg
     7681 caatggtctt gcaaggcacc taagaatccc gcttgatgca gccaaagcga tacaggtgat
     7741 aaataagctt agggttgaat caatagatta cgggctcgta aacgggttac cattcttttg
     7801 tacctgtggc gtgggttttg atgctcatat tggttaccgc tttgccaaag agaaaggccg
     7861 aggttttttt aactatgtta aggtagcgct aactgacttt ataaggtaca aacccaagaa
     7921 gtacaaggtt aaaattgatg gcacacaaaa aatgaaagtg aaggcctttc tcattacatg
     7981 tgccaacgca tcgcaatatg gcaataatgc ctatatagca ccaaatgccg atatacaaga
     8041 tgggttactc gacatagcca ttatgtcgcc cataaatctg cttcaagcac ctgccattgg
     8101 tgcaatgcta ttcacccgaa ccattaataa aagcacggtt ctacaaaccg gtaaggccca
     8161 aaagattgta ataaagcgaa aacgcgaaga tgtggttcac tttgatggtg aacccattat
     8221 aatggataga aaaattagga ttacagtagt gaataagggg ttaaggatta ttgtacctta
     8281 acgaccatcc acatcgagca aacgggtttt ctttctgata gttcgtaaag tcaggtaaat
     8341 tacgccgaca ataataaagg caataaacca caccctaccc cacgaactta gctcaaggag
     8401 tttaccccca tttgaaacca catcgtgtat gtactgaaaa acaaaaaata gtagcactaa
     8461 cttgaaaagc cctatgctct cgcgcttaat tacattacgg atggaaaagt aattgtttgg
     8521 tgagcgccac ctgaacctgc gcggaataaa agtgggaacg ctatcggccc aattgaggta
     8581 atccgaacca aacttgttgc gtaggaaaaa ttcctcggcg tacattattc gttcgtagta
     8641 gagccagtaa actaaaagga aagatatgac aaaccagtag ctcttagtaa gaagggcaag
     8701 gccaaaccac atgaaaaaat taccaaggta aagcgaatgg cggactaacg agtacattcc
     8761 tgaagtgtta accgtatcgg caacctgctc cttggtattc cttcctgaag tattcttagg
     8821 agtgtaacca atggtaatag cgcgtataac cagtccaata aagctcacca aaaagcaggc
     8881 aaagaagtaa aatgcattat atgaatactg cttggttacc aggaggtaaa catagtaacc
     8941 tatacctgct aaaattaaaa agcaccggta aatagctgcg atacctaaaa agaaagtcgc
     9001 cttggcgacg gaatgtttca atcaatgcca tacaaatcca tttatagaat actggcgtaa
     9061 atatagcatt tgttaatata ggttaatact gtggggcaaa ttatttttgt ttcattttta
     9121 agtagatgtt agtgagaacc tttttggtgt acaaggggaa ttcaccattc atcatccatg
     9181 aatagtagct gggttcagcc tttaatactt ccacaacagg cttacctttg tgctttccaa
     9241 agttaaatac ctcaacccct ttgtcatcga gaataatacg gcctgcaaaa tcgacattgc
     9301 gattaaacga gctgaactcc gaaagaaaat cgacatcgtt ttttagctca ggataccgtt
     9361 caacctgagc gcataggatt tcgaaggtgg caagggtatc ggcttcggcg ctgtgggcat
     9421 tcaccaagtc cttatccagg taaaatttgt atgctgcagc cagggttctc ttttccattt
     9481 tatggaaaat ggtttgaaca tcgataaact tgcgcttttt caaatcgata tcaaccccag
     9541 cacggaggaa ctcttcggcc aaaagaggta aatcgaactt gttggagttg aaaccagcaa
     9601 aatcgcaacc ctcaatatag tttgccaacg attttgctat ctccttaaag gttggcgcat
     9661 cttttacatc gtcatcggtt attccatgaa cagcagtagc ctcaggcggt ataggcattt
     9721 caggatttat gcgcctaacc tttgattcgc ggttcccgtt agggaatacc tttagcacag
     9781 caatttcaac tattctatcc ttaacaacat caattccggt ggtttccagg tcgaatacta
     9841 ccagcgggtt cttaatattt aatcccatta aaattacctt ttattaataa aaaaacttcc
     9901 ccaagccaag gccggggaag ttattgagtt tattcctaag caccaatcat cattggcatt
     9961 agaagcataa ggacatcttc gtcggggttt tcattttggt gagggaaaat gagccctgca
    10021 cgcgagggat cggacatgga aagtaccacc tctgttgagg aaaggtttgc cagaatctcc
    10081 accaaaaagg ctgatttaaa gcctatctcc atttcatcgc cttggtaatc gcagtttaag
    10141 cgctcgtagg ccgaaacgga gaaatcaagg tcttgagctg aaacctcgca ctggttcgat
    10201 gttaagccta gcttaataag gttgctggct gcgtttgaat agattgaaac gcgacgaacc
    10261 gagttataaa aatcaaccct gtcaataatc atacggtttg ggttatcaac aggaattacg
    10321 gcgctatagt tggggtattc cccttcaacc aaacggcaaa ccagggtgta accgctcatg
    10381 gtaaagcgag cattcttact atcgaactca acatttactg agccggtttc ctttacaaga
    10441 attgacttta gcaggttggc gggttttttg ggtagaatga acgatgaaaa ctcttcaacc
    10501 tgaacatcct tacggcggta gcgaaccagc ttgtgtgagt cggaagcaac aaaggtcatc
    10561 ccttcggtgg tcagctcaac aaatacgccg ttcatcacgg ggcgcatttc gtcgtcggcg
    10621 gtggcaaaaa tggttttggt aataccatta agcataacgt cggcagccac ctttacacta
    10681 actttactct ctgggctaag ctcagcgggt tttggaaaat catcggcggg ttggccaaca
    10741 atgttgaact ggccattctc cgagtgaata acaactgcaa gggaacctgg gtcaatttcg
    10801 aaagtaaggg gttgctccga gaattccttt agggtatcga taagaatttt aaagggaatt
    10861 gcaattgaac catcacccag aacgttatcg agggtaattt ttgttgtaag tgtcgattca
    10921 aggtcagatg ctgtgatttc cagctcatta ccttccaact tgaataagaa gttgtcgaga
    10981 ataggtaggg tgttcttgct gctgattacc ctgcttacca ccgataagtg gctcagtagc
    11041 atggtgcttg atacaacaaa tttcatatct ctgtaattta gttagttcct tttacctaaa
    11101 ttgattatcc tttagctgtc aaacagttaa ttgtatattt tagtttaaaa attgaaaatc
    11161 aatgcaattc aaatctcaaa aggatttcaa taatacaaaa ttttactcta ataacacaca
    11221 ctatttttca taattattta caaacacatc gatggggcat aaaacgtaat ggaagtgcag
    11281 ataggagcca accaaaaaac ggttttccga tagctttacc aatagacgat ttgggttata
    11341 gcttagttcc tgtccaagga tatttgtgaa tcctttacca gctatacggt aaaatgtgta
    11401 ttcgtaagtt tttccgttta gcattttgag ttttaagctg gaaattatat tctcaggtat
    11461 tgcagaattt gtttcggcct ttgttggata aagtaacttt aaattatcga atgagtagta
    11521 aagattgcta atgttggcaa gattaatgtt tgacaaccta tctcccgctg aattaattaa
    11581 tgctatttgg tttccaccaa gttcaatggt gtaccccttt gctttgtcag ggaaaatagc
    11641 cttgatttct tcaatcgcct caacaggctc gggaaacagt ttgccagcaa gccatttatc
    11701 agggttaacc gaaaagtatt caactaaatt atcaactgca tttgccgact caacgtagta
    11761 gggaacctcc gagccactgt tcagggcaac agttttatag ttgaattcag caactgaggc
    11821 tattctgtac tccttcttta cacgcttccc tttataaatg aaaaccccta tacccttccg
    11881 ttgaaacagc tggattaaat tttcatcggt ttttagggta gctggtgcta tagccgatat
    11941 actctgcaaa atatcgataa aattgcttac aacggctgaa tcagctaaaa agaaatcatt
    12001 tataaaccag ttgccatcgg atttggtcaa cctaagcgtg tcagatttca taaccagttc
    12061 aatagcagtt atttgagaac ttgaaaagga aaaatccgat tgacggaact ctttatacca
    12121 acgggaaagg tagaatagcc ctatacctaa ggctatgagt agtgaaagaa aaataatttt
    12181 tttacttgac atttgtaaaa gtgtatttct tccgtctaac aaaactccaa ataattccag
    12241 ctaaaacaag cataagcgat ggtaacacca tgttaacgag ctgccagtac aacctttcac
    12301 taacagcccg tttcctattg agaagtctaa gcttaacatc tctcgaacgt aaatcgagca
    12361 gtccactatc gtcgcataag tacttaacca tgttaaccac caactcctta tttccgtagg
    12421 tttgctgagt aaacctgtcg tagcccaacg ggataacata tgcgccattt ggacgacgct
    12481 gaatatcgtt tcgaatgata tcggcatcag ctactacaat cattttggta aaaatactct
    12541 tgtcatggaa cttgatggtg ccatttggaa ttattgcatt tatggggcga ttccgaaagt
    12601 tggacttgaa aatcccttgc aactcaacgg caacaggaag cattgaatgc tggaactcgt
    12661 aacgggtaat acccttgctt acctgattta ggttaattaa tgcaggaatt ttcacaagtt
    12721 tagcttcctt agatgtaaac aaaagtatcc gcttttcaac ctcatcatcc ataccaacgg
    12781 tatcaattgg acttataaat ttggtttgaa taaggtttaa acccttagta atagggttat
    12841 cggctggggg taagagcaat ggatagtaca gccagggtgc gggaacaaaa cggggctcct
    12901 gtccaacaag ggcagtattt accggaataa cagcacattg aatgtcctga atcagcaagg
    12961 gattaaggcg aacgccatag cggaaaagca tatcatcaag attatgctga ttcataaaag
    13021 ccagggtaga agcaccagat gataagctat cgatactaac gttaacgggg tcaacgaacc
    13081 atagcacacg tccaccctgc ataatgaact ggtcaatgat gagcttatcg gcctcaggca
    13141 atggcctgct gggtcctgca ataataatag tcttgtaggg ttttaaagca tctgtgtcgc
    13201 catttataat cactctgcta acatcatagt attgagccag ctcacgctca atatccccag
    13261 tttcaaactc gtcaaactca ccatgtccct gaataaaagc aatgcgctgc ctgtgttcgg
    13321 ttgttatacg gtcaattgca tcaaccaggg cgtactcaaa gttttgaatg gataggttga
    13381 tattctcatc gcccgtaaga tttgggttat tccttaaaag attcaccgct aactcctttc
    13441 ccctgtatgt aataatacaa cccgggaaaa tgatgcgctg aatcattgca ccctcattat
    13501 ccttaacatg aacgttggtt ggaacaagtc ccttttcggc caactggcga aacaattgct
    13561 cctgcttttt tgcccccgat gtagagtatg gattgacaaa ttcataccgt atttttttcc
    13621 cggcaatctc ttgaaattca cttagtgtct ccgcaagggc ttttttcatt ctaacaaagg
    13681 caaccggcag ttccccatcg agatacaccc tgataaccac aatatcatct aaaccctgta
    13741 atacctcgcg gctaacaggc tgtatggtaa accttttatc ttcggtaagg tcgattcgga
    13801 agtaaaaaaa cgacgatagc gcattcacta aaagtagtat gagcagtaaa acagcaaatg
    13861 ccctgatact tctaattttt atttttccac taacactcat aacagtccat tttttggttt
    13921 taccacctgc gcgattgaag ttttaaacgg gtaagagcta aaaaaactga tgatatggat
    13981 aaaaagtaaa ccacatcgcg cgaatcaata acaccccggc taactgagct gtaatgctca
    14041 cttatgctaa gagtcaacaa tgccgaccgc gcccactcca tggattgaag ctcggctacc
    14101 caggctaatc catcgtaaaa aacgaagcat aatgcaacag ccaaaaggaa cgcaacaatc
    14161 atattatcgg ttaaactcga ggcaaaaacc ccaagtgctg cataggctga tgctaagaaa
    14221 aatagtccga tgtatgagcc ccaggttgca gcccaatcca gatttccgga aggattcccc
    14281 aaatttgaaa cgctataaac aaaaatgaat gtgggtacaa taactatggc aacgagcacc
    14341 aacgacgcaa agaacttccc cagtaccatt gcggtatcgg aaatgggtcg agtaagaatt
    14401 agctcaatgg tacctgtttt cttttcatcg gcaaacatac gcatagttgc cgcaggcaca
    14461 agaaaaagga aaagccatgg tgcaatgtag aacaacgact ctaatgttgc ataccctgaa
    14521 tcgagtatgt ttgtgtaacc gggcagcacc caaagaaaca aaccagttaa aacaagaaaa
    14581 ataattgcaa taacgtaccc ggttaacgat gaaaaaaagt tctgaagctc tctaataaaa
    14641 attgccttca tggatgcttg tgttttagga taaaattact gtaacacatc aaaaggctta
    14701 acctcaaggg tgtcgggttt taaggaatca aggttaacct tggaaatatc aataatttgg
    14761 atatccttaa agtaaaactg gattatctta tcgtatgtga aaccgcgctg ggccatagcc
    14821 aatgcgccat cctggcatat tcccacacca tgaccgtatc ctttacccga aagacgaacc
    14881 tctgattttc ctgctgaaac cgtaaaccat gcggaacgca acttaaaata cttccgaacg
    14941 gcagttgtag cgatatttac acctaaaaca gtatagtatt taggcctctc cttggggtaa
    15001 aagttaaggt tatcgcgtgt taccttagcg gtgtcaaccc cttgctgagc aagaaatttt
    15061 ttccattcgg taagaggtat tgttctttcc cagcgagcgt taggtgccga tgtgcaatac
    15121 ctatcgttta cactcctgag gtagggcaat gcctgaatcc aaacatcctc ggagtttgct
    15181 gtttgcccac cacagtttgc gtgaaatgca gcgctaattg gctttaagga agaatcaaca
    15241 acaattaaac cctttacatc aagaactgca tcgtaaattt cttgaaaagc ctcaggcctt
    15301 cccttgtatg cctgacagtg ctcctgatcg caaaggttaa aaccctccga ctggtgttta
    15361 tcgatatggg taacagtaaa tgtccgggca agtaaggctt gcgccttata caattcgggt
    15421 tccttattcg ggccggtttc agcaagaaca acagctgcaa tatacttttc gcgatcaatt
    15481 atgttgaccg ccataatacg attaaactca acgtaaaagg taatattatc gtcgtataaa
    15541 cgagccggtt ttgcaggaat tattggctta aggcgaaaaa catcatcgcc ggtttggcca
    15601 ataagcgtga cccgcttgaa tgaaccatag ctttggttct cgtcgcgcac cagaacagaa
    15661 tcgccttgac ggctaatgta cagtatttgt aatggcttaa ttgtaatgat actatcgcca
    15721 agtaccatgc tgtactcacc cttagtgggt gtaacaagta ttgtatttaa attagtatcg
    15781 ttaaaaacgc ttatgcttaa cttttgcgag ttcgatatga atggcagaaa aagcaaaaac
    15841 gaatacagaa ctaccctcat aagcaagtat ttaagatgat tttatcaaat ccacaatcct
    15901 tttacccacc cgttccgatg cacccatctt accaagtttc tccttgagct ggccataaag
    15961 ttccagctga cgatctcgtt ccggattgcc gggtaaaagt tttatggcct cggtgtaaag
    16021 actatctggt gttaaatcgt actgtttcaa ctccctaaca acctcaaacc ccatgttcag
    16081 gttcaccagt gagatatact tcaccttaat aaccatccag gcaattagca tggagaagaa
    16141 gttcccccta taacaaacca cctgtggaac gttcagtagc gccgcctcaa gcgttgctgt
    16201 cccggaagtt acaaaggcaa gtgttgagca tgagagcaac tggtaggttt gaccaaacac
    16261 cacacttaca ccagtaccac taatataagg ctggtaaaca ctataatcca acgatggggc
    16321 accggctatt acaaattgat actcgggaaa ccttttggtt acattcaaca tcacaggcaa
    16381 attgtagcgt atttcctgct ttcggctacc tgcaacaagt gctattattg gtttttgagg
    16441 taatccattt ttttcacaaa aatcacttaa agaaattgga ttaggaagtt tgttagcaat
    16501 ggcatcaatt gaagggttac cctcgtaaac ggggtcaatg cctttacccc tgaaataatc
    16561 aatctcaaaa gggaaaatca caaaaagttt atcgacaaag cttttcagtt tctcaacacg
    16621 cgattccttc caagcccaaa cctttggcgc tatatagtaa aacaccttaa atccggatag
    16681 ttttgcaaat ttagcaatac gtaagttaaa accgggataa tccacaagta ttactacatc
    16741 tggcctaaag tgaataatac tttgtttaca gagctccatg ttagccttta tggttcgtag
    16801 gttaagtaaa acctccaaaa aacccataaa ggccatatcg cggtaatgct ttaaaagggt
    16861 accaccctgc tcagccatta aatcgccacc aaagaaacag aaatcggcat caggatcatt
    16921 atttttaata cccttcatca gatttgatgc atgtaaatcg cccgatgctt cgcctgcaac
    16981 tataaagtat ttcattttaa tctacataaa acgcatgaaa acaattatta aagcaagaat
    17041 tatggtgcta cccagtacac cctgagcggc cttgagccta tcggtccaaa taaatacaaa
    17101 aaacaggcca aggttgggta aaacggcaag cgataatgct ctggacaaat cgcctcgtac
    17161 gtttaaatat ctaataaaat cgacaaactt aaaatcgctg gcaagcgata aatacataac
    17221 taccagcgtg atgacgggca gcaaagctcc aagtaggtat ccatgccact gtttacttat
    17281 ttttttctgc atgcgaaatt gcaatttggt caaaccgcca cgattttata cgatcgagtt
    17341 ctttgtaatc ggtcaaatca acatgaactg gcacaactgt gatatacccc tggtttagcc
    17401 agtactcatc ggtatcggtt gcgttgggtt cctcgttcag gaagtatccg gttaaccagt
    17461 agtaatcaac acctctggga tcggttcgtt tttcaaactc ctcgcgccag gtgcctttat
    17521 tttgtcgtgt aacctttacc ccttttactg actttaacgg caaaacagga acattcacat
    17581 tgagacacga gcctggagtt aacccatttt caagtacgtt tttaactata aaccttccat
    17641 actcaatagc agcagtaaag tcggggtttt cgtcgtaatc aagaatagaa aaaccaatgg
    17701 aaggtacacc gtacaagcat ccctctatgg ctgcagccat ggtgccactg tatattatgc
    17761 taatggatga gtttgagcca tggttaatac ccgatactac caagtcgggc aacttacttg
    17821 taaaaatctg attcatggca agtttcacgc aatccacagg agtaccgtta acggagtaga
    17881 attcaacatc atcggtacgt ttacgttttt ttaacctcaa gggagtttta atggttatag
    17941 catgcgacat gccggaatgc ccttcctccg gagcaacaac cacaacattc ccaaagggtt
    18001 ttaccatttc aacaagtgct tcaataccgg cagccctgaa tccatcatcg ttggttacaa
    18061 gtattgttgg tcttttcaac tcaccgtttc cctcaaacat ccttttctga tttgtgttaa
    18121 ttggcaaaga tataattttt tagacagtaa aactcctcag ccggcagggt tgttttgact
    18181 tttgcaaata ttaacgtcaa gcaataattc tttgttgttt acctcaatca tgcatcaaag
    18241 caataaaaaa gttcggccga ttgttcctgt ggccgaactc aattaagtat taagtcaaaa
    18301 cactaaaaat ccattcttcg agtaattact ccgggtattt taaccagttc gtcgtgtaat
    18361 tttgcttgtt catcggcggg gccagtaagc tcaagtatta ttaatcccga ggaactgcag
    18421 tagtcgtcag aaacctcgtg taaacccaaa cgggttttaa ttgagcaccc gtaacgggtt
    18481 aaaacccgtt gaacctcgcc ggcctcctta attctatcgg taattttaat acccaaaatt
    18541 gtgtggttgt tttccatagc taacagggtt tttggtttcc ataaatttag gttaaaaatt
    18601 gaaacatttc aagtgagatt tggttaaatt tatgtgattt aggtgcaatt tttgtggtaa
    18661 aaaacttacc ttgcagactc actgattaaa actaaaagct taaataaaat gaatagaagt
    18721 tattctattt caatcctttt ggcacttact ttcattagtc aaattgttta tggccaatac
    18781 tttggcagaa ataaacccat ctacaagaat tttaagttta ccgtttatca aaccccaaat
    18841 tttgaggttt accattacct gaaaaacgat agtctactta atatcatatc gctttcagcc
    18901 gaaaagtggt acgaccggca ctaccaggtt ttcagggatt ccataataac aaaaaaccca
    18961 atactgctct ataacgatca tgccgatttc cagcaaacca cggctatatc ggggagcgtt
    19021 ggagtgggaa ctggtggtgt aacagaggcc ctgaaaaacc gtgtaatctt cccattaacg
    19081 gaaacgtggc agcaaaccga tcatgtcctt gggcacgagc ttgtgcatgc ttttcagtat
    19141 aactctttga ttaatggcga ttccacctcg ctcaactcag ttcggaacct tccactctgg
    19201 atggtagagg gcatggccga gtacctatcg ctaggcacat acgactacca aactgcaatg
    19261 tggatgcgcg atgctgtaat taacgacagg tttcccaccc ttgaggatat gacctactac
    19321 cccaacaagt acttccctta ccgctgggga cacgcattct ggtcgtttat tgggcgaaca
    19381 ttcggcgata gccttataaa cccaatattc aaggaaaccg caaagcgggg ctacaacttt
    19441 gcactcatgc gttaccttgg gttgaacgag caatcgttct caactctctg gaaatcggta
    19501 tactatgact attttaagcg taccatgccc gatacggttg aagatcctaa cggaacccga
    19561 atcctttttg aacgaaatgc aggcgaaata aacatttcgc catcggttag ccccgatggg
    19621 cgatatgtgg ctttttactc cgaaaaggac ctcttttcta ttgaccttta cctggcacat
    19681 gccataagcg gtaaaattat tagaaggtta tcgagcatag tgcacgataa tgagattgat
    19741 gcactcaact ttattgagtc ggccgggaca tggtcgcccg atagccgaat gtttgccttt
    19801 gtaggattta gcaagggtaa gagcaaattg attattgttg atgtttttga tggtaaactc
    19861 attcaggagt tcaccatacc cggtgttcca tcgttcaact atcccgcctg gtcgcccgat
    19921 ggtagtagca ttgtggtgtc aggacttgta aatggggtta acgatttata ccagtacgat
    19981 ttaagaatta aaaaggttga ccgcctcacc aacgatccct ggtgcaatat acatcctgca
    20041 tggtcgagcg atggcaaaac cattgttttt tcaaccgata aaccctcaca aacatcagct
    20101 aacggaaaaa cagggtataa ccttgccaca ttagatgtag agactaaaac cattaatgtt
    20161 ttcgatattc tccccggtgc cgataatgta aacccaatgt tttcagccaa caacaaggaa
    20221 atttactttc tttcaaactg cgatggatac cgtaaccttt tcaggtttaa cattgaaagc
    20281 ggcgaagttt acaggatgac aagaatttta accggcatct cgggaatgac accgcttgca
    20341 ccggccatga gcgtagcccg tgagaacgat gaggtggttt actcccattt cttcaaaagc
    20401 aagtactcca tatatactgc cgatagctcc gattttaaac cggttagaat aaatccatat
    20461 caagttgatt ttaacgcatc tatccttcct ccagtaaata gggcaactga aggtttggtt
    20521 gataaaaatc tctcggataa aaacctacct gtggtcttgg tcgattcatt taaaaccgta
    20581 ccgtttaagc ccaagtttaa gctcgattat atttccaata ttggcatggg tataagtacc
    20641 tcaccatacg gaacaggtat ggctggcggg gtagaaatgc tttttagcga tatcactggc
    20701 aaaaacatgg tgtataccgg attgtcgctt aacggcgaaa tatatgattt tggagggcaa
    20761 gtagcattcc tccagcaaaa aaagtacatc acttggggtg taacagcttc ccatatcccc
    20821 tactctttcg gacaatacgg gtacgatact ctccacaggc aaataaatgg cgagcctgta
    20881 atagttgaag attttcagct cattcagata cgaatgttcg aaaccgccac gggactactt
    20941 tcatattttc ccttatcatc cacacagcgg tttgaactaa gtgggtcaat agcccgctac
    21001 tattacagag tcgatatcta ctcaaactat tacattgatg gctactacta cggttccgaa
    21061 cgcaagaaag gggaagtacc accgggcttt tggttacaac aatcgagttt agcatatgtg
    21121 tacgataact ccgactttgg tatagcctcg cccatgcgtg ggcacaggat gcggttccag
    21181 gcttcaagaa cccttgggga atataactac tggggcgcct tagccgacat gcgtaaatat
    21241 atattccgta aaccgcttag cttcgctttc cgcgccctgt actacggtcg ttttggcaca
    21301 tcaataaacg ataacatact atacccgcta tacattggct acccttggta tgttaggggg
    21361 tacgatagca attcatttat taactatggc caatcgggca atgtaaccat tgaccagcta
    21421 accggaaacc atatagcggt tgctaatttt gaaatacgag ttccttttac aggccctgag
    21481 cgcctaacct taattaagtc gggtacattg tttacggaac tggcattctt taccgatgca
    21541 ggtatagcat ggacaaagga gtacccgcca agcttaaaat ggaaacccac ctccataaac
    21601 gaccgaacgc cttttgtaag ttcagggatt agccttagga taaatctgtt cggaatgctt
    21661 attctggaac cttactatgc aataccctac cagctaggcg gattcaaggc ggcaaactgg
    21721 ggtatcaact ttattcccgg gtggtagtgc taaaccaccc ggtttttcca tcggccagtt
    21781 cttaagtaaa taattgagat tactgtaacc agaataccat atagcacctc cgaagtccaa
    21841 actaagctta aggatttagc taaaattatt gctataaggt aagcccagcc taagtaaaat
    21901 gtcagtgtaa acacctcaat ggcaaacgat accttagtgc taccggtacc tgaaacacca
    21961 ttgaaactga taaaggccga agccattgtt aaagcagcta ttgcgattac cctaataaca
    22021 ggcagggtgg cattgattat gtgctcatcg ttagtgtaaa tcttaataat gctatctccc
    22081 agcaaaagaa ccagagccga aaggacaaga actgaggcta aagtaatgac cattactttt
    22141 acaaccagct ttaccacctg atccgactta ccttttccaa tatgttgact tacaaatgaa
    22201 ttggtagccg aggcaaatcc ccaaattggt atcataagga taatgtaaat gcttcgaact
    22261 atgttggaga cagcaagcgg agtttcgccc attttttcaa taataaggaa gaatacaaac
    22321 catacaaaaa ttgatagtga aaattgaagc atcaagggcc aggaaatatc aagtattgac
    22381 ttcaataact tttttgaaaa cgacgaaaag ttgaatagct tatacttact tacattcatt
    22441 ctgcttatgt aaacaagtaa aaatgtagcc cctgcaattt cggcaataac cgatgctagg
    22501 gctgcccctc taacacccat ttgtggtagt ccaaagttac cgaatatcaa tccgtaatcg
    22561 agaacaatgt ttacaaatac tgtaatgaac gatgtaagtg taatcacctt tgtacgtgta
    22621 attcctatta ggaatgcgtt caatgagtat gtgagaaaag caaagggtag cccaaatagc
    22681 ctatacccaa aaaaagaact tgcagaatcg catacaagat ttgacctaac agcaatacta
    22741 aaaaaagccg gatgaagagc atgtgcaaat agcattataa gaatagcaaa ggcgccgaga
    22801 gtaaaaagta aatgctcaac tacacggcca acagcctcat ttgccccctc gcctacacgc
    22861 cttgccacaa taatttggcc acccatagcc attccccagc ccaacataag caaaacatgg
    22921 taaagcagcc ccccaagaac acctccaccc aatgcaatct caccaactcg tcccagaaaa
    22981 gccgtatcgg ttatgttcag aatattttgg gcaacgcttc ctaaaattat gggatatgct
    23041 accctccaga tttccctgta ggaaatatcg agtttcattg gaaaatattt gtgtaaaaat
    23101 acaaaaaggc aggggattcc ctgcctttta ttgaaccctt cgcactataa attatcgtcg
    23161 aagtatcgta taattttatc aatcaaatgc accctatcct tccccattac attatggtcg
    23221 tgttggggat acataaagaa atcaacctgt acgccatttt taataaactc atgaattaag
    23281 gtgaggctat gttgcggaac taccgtttta tccatatcgt catggataat aagaagcttg
    23341 cctttcaggt ttgttacata gttcttaagg tcggcttttt tgtatccatc tggattctca
    23401 tcgggagtat ccatgtaacg ctcaccatac atcacctcat agaatttcca gtcagtaacc
    23461 ggaccaccag caacaccaac cttaaagtgg tcggatgccc taagcatcat ggttattgtc
    23521 ataaaaccgc caaaactcca cccatgaacc cctaaccggg ttgaatcaac ataaggaagt
    23581 gattttaagt aattaactcc tacaagttga tcgccaagtt caaccgtacc caattgccta
    23641 tgaatagctt gctcaaattg agcgccacgg ttcgaagtgc cacgattatc gagtgtgaaa
    23701 agaatatagc ccttgttagc catgtagcac tcccaaagcc ttgcgcctcc catccagcta
    23761 ttggttacca gctgcgaatg tggtccccca taaacgtata ttacaactgg gtacttttgt
    23821 gtagaatcga aatttactgg ttttatcatt cttccatata aagtagttcc atcagttgcg
    23881 gtaagagtga ttaggtttgg caacgaaaca ttatagccag tgtatgggtt tggcgcatca
    23941 attaactgtt ttaccagttt acccttaatg gtgtgcaatg caacccgatt gggaactgtt
    24001 attgagctat aaaaatcaac aaaatactta tagcttggac taatcatgca gcggtgagtc
    24061 cctgagctcg ttgtcaactt tgtaatagag ccatttttag ttgaaacaga gtataaatgc
    24121 ctatccaatg ggctctcctt ggttgacgta aagtaaatca agccattctt aaaatcggtg
    24181 gcatctatac ttgtaacctc ccattcccct ttggtgagct gtttaacaaa cttaccatca
    24241 gccaaataca ggtaaatatg attccatcca tctcgacggc taagccacaa aaatgagttg
    24301 ctgttaatga aaaaaggcgg ttcaagtggt tcaacatact tactactgct ctcctcaaac
    24361 agggttaact caaaatcgcc agtgcttacg ttgtaacgat tcatgcacat gtggttttgt
    24421 tccctattca gaacagcaat cagtatggaa cgctcatccg gagtccatgc aatattggtc
    24481 aaatactgat cacggggttc cccggttttt agttcaaccg ttgaaccgct ttgagtatca
    24541 taaaccctaa cggttaccac atggctttgc atgccagcca taggatacct aatgttcacc
    24601 aactcgccaa cacgctttga aatatcaacc aatggatatt tggtaaccat actctcatcc
    24661 atgtggtaat atgccaggta tcgtccagaa ggcgaccaaa acgtaccctt gttaattcca
    24721 aactcatttc ggtgaacact ctgcccgtta acaattccat cagtagtatc gtttgttact
    24781 gtaataacct caccctgagc atttgtaagg aaaaggttat taccaatggt atacgacatc
    24841 cacctgccgc cagggctaaa ctcgtggttt tcagaattat cggccacaag aaactttgac
    24901 gcaatgcttt tggttacaga gttatacaat acaaagtagt tacctgctat gaagcggagc
    24961 tgcttgccat cgccccaagc cgatggtacc ctttttagag gctggaggct attggccttt
    25021 agagcctcgt ttacttccga taaagttaca gctctgtttt tctttgagtc agtaacattg
    25081 acaatgatta agctgtcgtt gctaacaaag gaatactcat cggttccagg caaccagcta
    25141 aaaccgggta gggttttagg gcgaaactct gtgccctggc gtaacacagc ctgctccagg
    25201 gtaagttcct tttgcccaaa agctgtaaat accagtgtaa taatcaaaaa ggttgaaaaa
    25261 aatcttttca tatctaaaat ttttaaggat tgcactattg aaatttaagc taaaatagtt
    25321 aactttattg tttaaattga tactaaaaag tttgtatggt ttaaaaagta aagcagaaac
    25381 ctacatattt caacatttgt ggtatttttg cttagtaaac atttcgttaa aaagctattt
    25441 ttcttttatg attgaaacac tgacaatgag cttacgatta cccaaatcaa aattagccct
    25501 agtacttata agcgcttttt tagcatcctg taaaccctca cccaaacagg ataacgaaac
    25561 aacccaaccc gcagccgata cgctatcgac actggttaag gttgaaaatg ccattttccc
    25621 aattccttcg ccctggcagg tggttaacct gattcaaaag aacaatatcc cattcaatga
    25681 atcatgcatt aatagtacaa aaaattatca gcgctacact acctcattta agcaagcggt
    25741 aaaccttggt atttatggta ccgacttaag ctaccttaac atatacgata aaactcagga
    25801 gtcgctccaa tacctaagtg taataaaaaa gctaagtgag cagctaagaa tcactgaatc
    25861 gttcgacgaa aagatatttg aaaggattga gaacaatatc gaaaataaag actcactgat
    25921 aaaaataata tcggatacct acgcaagttc tgatttgtac ctgaaaaaga atgaacgtta
    25981 cgatataagt gcattggttg tagctggttg ttgggtggaa agcctgttca ttatgacaaa
    26041 aattgcccag gagcacaacg atagagagat tattaaccgt attggagaac aaaaacaccc
    26101 actcgataac ctggttgagc ttttaacgcc gttctactat aaatcaaccg aatttgccaa
    26161 tttgctcgac agccttattg accttgccta tgagtttgat gggataatct actcctacac
    26221 ataccgcgaa ccaattgttg acgaggcaaa tagggttata accattagaa gtcaatcgcg
    26281 ggtggtaatg tcagaatatc accttaaaat tatcagcaaa aagattgagc aaatacgctc
    26341 ttcgctaacc gaataaatat tccaatatgc agcgcatagt aatcatatta accctaacac
    26401 ttttcagcct aaactcattc tcacagattg agcagctaaa aaaaacatgc tctatcaatt
    26461 tcccatcgaa ctacataagc gatgggcagg aatactttgc agcccttaaa cctgaccagc
    26521 ggattgagtt tagaacaacc ttttatgccg ataatactta tagaatatcg gcctgcacca
    26581 atgccaaaaa gggaaaactg gtttttaccg tttacgatac cgaaaagaac ctactgttta
    26641 ccaatgaaag ctttaactac tcccctttct gggattttcg ctttacatca acagtaacct
    26701 gcataattca ggtagatgtt aaaaatcccc aattcattcc aggctacgta atgctactaa
    26761 taggctttaa gcagtaaatt cctgtaaacc atgagcgagt cgattctaaa agctctcatg
    26821 caactttttg caattattgc ccgcccggtt tccctggacg agaaggagaa taacgattat
    26881 gccgaccggc gaaaggttgt ggaagagttc ttgaggaatc agcttaatat cgacctggtt
    26941 aaagagtacc ttaaggtttt tgactactac tataaccttt accaggaaaa gcaaagcgag
    27001 aaaagcaagt caaaaaagag aacatcatcc agctcggtta gggttcttaa gatatgctcc
    27061 caaataaatg aggaactaca gcaacaacaa aaaattgttg tactaatcag gctacttgaa
    27121 tttgtaaaac ctgacgaagg cgaggttact gaacaggaaa tggctttcat tagcactgtg
    27181 gctgaaacct tttacgttcc tgaggacgag tttcaaaacc ttaaggattt tgtgctctct
    27241 ggtttcgatt cagtaatccc gcaccagaat atcctgctta taaacgactc taaagagcct
    27301 ccctatgacg atttgaggca cctttacgcc gaaggccttc atgaacccat aaaaattctt
    27361 tttgttgagt cggcaaatat ctttttccta cgctacaatg gcttacacga gctttaccta
    27421 aatgggcact tgctggttaa ggataaaatt tttgttttaa ataacggttc atcaatccga
    27481 aattcaaaga taaagccaat atactttagc gatattgttg gccgctttgt tcaggataag
    27541 attaaagaga aaatcgtttt tgaagccatc gatatcgaat accgattcaa aagcggaaag
    27601 attgggttgc accccatgtc gtttgttcag gagagtggcc acttggttgg gataatgggc
    27661 gcatcgggcg ccggtaaaac cactctactc aacgttttga acgggagcga gaagccaaca
    27721 aaagggagta tcctaattaa tggcattgac atacacaacc aaaaggaact ggttgaagga
    27781 caaataggat ttgtttcgca ggacgattta ctgattgagg agctaaccgt ttaccagaac
    27841 ctatacttta atgcccgcct ttgctttgat aactacaccc atgagcagct cgaggctgct
    27901 gtaaaccaaa ccctacaaaa ccttgggcta tatgaaatca aggatatgcg ggtgggttca
    27961 cccctaaaca aaaagattag cggtgggcaa cgcaagcgac ttaacattgc gctggaactc
    28021 atcagggaac cggcaatcct tttcctcgat gaacctacct caggattaag ttcaagggat
    28081 tccgagaaca ttcttgacct cctaaaggaa ctaacgctta aaggcaagct ggtttttgtg
    28141 gttatccacc agccctcgtc ggaaattttt aagatgtttg acaggttact tatactggat
    28201 acaggtggat accttattta caatggcgat cccattgaat ccattatcta cttcaaatca
    28261 cgagtacagc aggccaactg gaacgagagc gagtgccccg tttgcggtaa cgtaaacccg
    28321 gaacaaattt tcaacatagt tgaatcgaat gtgctcgatg agtacggaaa cccaaccaga
    28381 gctcgaaaga taagcccttt tgagtggaac gagtacttta aacgatttgc ccctgaaaac
    28441 cctaccccta acacacaacc aaagtcactc cctcaaattc actttaaaat tcctaactgg
    28501 gttaagcagc ttcgaatttt tgtaaagcgc gacattttaa gcaagctggc caatagccag
    28561 tatatgataa ttaatatctt tgaaacgcct ctacttgctt tactattagc ctatatcata
    28621 aagtactaca acgttgacgt atcaaataaa cttggctata cgctcctaga aaacagcaac
    28681 ttaccggttt acctattcat gtcagttatc gtaggaattt ttgtggggtt aaccgtaagc
    28741 gctgaggaga taataaaaga tagaaagatt ctgaaacgtg aagcgttcct gaacctgagc
    28801 tggtcgagct accttatgtc aaaagttacc atacttctag gcatatcagc ctttcaagcc
    28861 ctgatgtttg taactattgg caacagtata atggaaatta aagggatgta ctttgagtac
    28921 tggctagttc ttttcagcac atggttttca gcaaacatga tggggttagt aatatcggat
    28981 agctttaaaa cggtagttac catatatatt cttatacctt tccttgtaat accacaaata
    29041 atacttagcg gcatcattgt taagtatgag aaactaaacc caaccatctc ctcgccaagt
    29101 aaaataccta tttacgggga actaatagta tcccgatggg cctatgaagc cttggcagta
    29161 taccagttca aggaaaacag atacatgaaa ccgctttacc gtttcgatga agcaatgagc
    29221 ttatcggact acaagcgtaa ctattggcta aaatcaattc agaataaggt tgatttctgt
    29281 atccgaaacc ttaacgataa ggaaaagaaa gccgaattca aaaatactct ggagcttatt
    29341 agaaatgaac taataaagga gatgactgct cccagggcct cacatcttat ttttagcaag
    29401 tacaccatgc taaccccgga ggaggttaat gctgatattc ttaaagagct taacgatttt
    29461 cttgagcagc tccgaaagca ctacgttaag ctgtataaca aagcaagtgc ggaaaaggat
    29521 aactatatta caaggttcca gaatagccaa cccaacggac aacagctatt cttagagcta
    29581 aaaaggcgtt atcacaacga aagtttaagt gagtttgtac gaaactcagg cgaggttgag
    29641 cgaatcattg agtataagaa tcaactaatc cagaagatag accccatata ccagttcccc
    29701 gaatcaaaat ttctacgtgc acacttttac gccccggtaa aacctttctt tggtaactat
    29761 ttcggtacta tttgggttaa tgtaattgtg atttggctat cgagcattct gatgtacttg
    29821 gtactctact accgactcct caaacgctta cttgagtggc tggaaagttt gggtggtgaa
    29881 acggaaagtt gatgagcttg atatgaaaaa aggggcaaaa taatttgccc cttacttttt
    29941 atactaactg ctgattaatc cactatctga atcatcttaa agggaacttt gatgattgac
    30001 tcgtaatcct ccccagcttc aagcatatcc tcatcatcct cctcgtagta ccagttaata
    30061 attacatctt tctggttttt cttaagaacc tcaagctttt taaaaacatc aagaatacac
    30121 tttgatgagg aagtgttgaa atactccaac tgaacgttaa caactgtatt tggctgggga
    30181 ttttttatgt attcatcgag ccattccaca acaggacgat agaattctat tgaattctca
    30241 gggattgatc gaccctttat ttcaattact ccggtactgg ggttaagtat aacggtcgga
    30301 gtttttggcg ttccctcaat gttcagtaat tccataatat tgtagtttta attattcact
    30361 taccaataca ctcaaattaa aaaaagagta ctcatcgtta tatggagtaa actcgtaatt
    30421 cagcttgtta cccgttttcc tagcaatatc aataaggcct aaccccccac cacctttttc
    30481 agaaaacgtc tgattattca gaacaaattt ataaaattct tttaactcat cctttgaaag
    30541 agaattaatc ttgtcaattt tttctttaag gatattactt ttatcgttac gtacaaggtt
    30601 accggtggca attctatacc catcattggt tttggaaagg ataaaagcac ccattctggg
    30661 ctcattctct gagagcgtta ctttatcaac atgatgaaag agattttgga ggcactccac
    30721 catcacattg taaagttttt tccgaaccaa gttttcaacc atctcgtgcg atagctttgc
    30781 ttctaccaat ccaagtgtat cggcaatgat gtttgcatta atttcaccct tataggctac
    30841 aattacatca ccactatgca ttttggcata gcattttgaa ataaaatcgc tcatcgaatg
    30901 gtttgtactt gattcaagca acacctatgt aatttatatt taaacaaata taataaaatt
    30961 ttaattgata aatgcaa
//
LOCUS       DAOSAL010000025        29616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_1157_length_29616_cov_47.824600, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000025 DAOSAL010000000
VERSION     DAOSAL010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 29616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29616
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1157_length_29616_cov_47.824600"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="PK049_05215"
     CDS             <1..527
                     /locus_tag="PK049_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK60989.1"
                     /translation="GEGEFNLSKSLETLGVPVVNLTENVPDWAKHSYVYIQDTFAINL
                     NEVYNQSDLISYIMIRVNIWNEFPLQGNAQIWILDNGQNKLDSLFAQPASVPAAEIYS
                     NGNVVGTKFESYKVDFDQNRVDLWQNAATAVVRAGLVITEEGVTEQNIQYFDRYKLKV
                     QLAARVDFKTIKNN"
     gene            531..1961
                     /locus_tag="PK049_05220"
     CDS             531..1961
                     /locus_tag="PK049_05220"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HOK60990.1"
                     /translation="MLLRFTTLSALFFAYTLAWAQQSNSLFFMHRIPQSNLINPAVQI
                     DCPVYVGVPFLSSLHLNLNSTGFSYNDIANGTGTINFPALVGQMHSWDYLSEELHYTP
                     VSFGFMYDNDQYFNFAWTERVESKLFVSKRLMSLFANGNTQFVGNGLITSNPGLNSFY
                     YREFSFGYSTRLRGDVLVGAHAKILFGLAGLYTKRNSVDIEIDALTHNINARWNPQVN
                     VAYPLGVTTDAAGNVTGVSAGAFSPLPFFLNFSNPGLAFDLGFVKPNDPITWSGSILD
                     LGFIWWFKSTSRFTNDGHFVYRGATPADLTNPDAYIDMLADSINNQVQFQHSNGSFVS
                     FLNPKAYFGGTYAFTPQLLAGAHVRTEWYPGRPVVGLTLSAIAMAKKGSSVSLTYSVM
                     NGSFANIGAAINLGGESFQFYILTDNLLAPFYPERVRNANLRFGFNLFWGCKQKKKKL
                     SIPESSGGGCFWTWGLDQKRKQHGVK"
     gene            complement(2010..5342)
                     /locus_tag="PK049_05225"
     CDS             complement(2010..5342)
                     /locus_tag="PK049_05225"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOK60991.1"
                     /translation="MGLFRTDKKLLPSCVAALLLAATSSVFAQRVNVTLFNSDNGLPQ
                     SFVNALLQDSLGFLWIGTQDGLCRYDGYDFVVFNNNPYDSLSLSNNYVNHLVEGVNGE
                     LWIATRSGLNRVNRKTGKVKVYYNHANNPRSLSSNMILRVYRDRSNRIWVKTIDALHL
                     YNPTTDDFTRFPHFNDIFNISQTSDLSPIFENSNERLWVGTKDGLFYFDKERLVFKRY
                     SEDPANPNSIWGNRVNAITEGLSGKLLIGTDKGINEFDFLTQKFRRVILTLNDPSNKS
                     LTTGIQFLGYDNDSSLWVGSVGGLGKLLPNGTFIKYNSIYSNNLPVNILNVSTVLRDR
                     TGVLWIGSQTGLVKVNPFEHRIGGYSKDPNGRNLFSNNVAASVIESTDGNIWVGTWGS
                     GLHIFNPTTGENRHYWSGGGNYIPNDFIHSLYQTRDGRVLVGTRNGVFQYLPSSRQFV
                     DFFERNNVYVGDLFKNNRVYSITEDLQDRVWFATRQGLHCLKGGNLISFYARRGDSLS
                     LPGNEVYDVVADKNANVWVATISGLCRISADLRTIETFSFIGNKSRLPFEVLCLYIAK
                     NETVWVGTTSGLFWINPKGKNVLHSVVLPDINIRLINDILEDDRGRFWISTNKGLVLY
                     NPLSNLVKVFNNSDGLFSNELNINASFKNSKGRMYFGSIDGVNVFHPDSIPTNKVVPN
                     VCITHIQVYSKSGNTEIIPYQLKFLRIPDDFSNLTFTIAALDFVQPNKNSYRYQLVGH
                     DSRWVELGNTNKITFSNLSEGDYELRIMGSNSDQVWTTEYKSLRIRAVAPWWRSRIAK
                     WIYGVIVLISLLVWAIRRNKHLREINRLLHERETVLEELRLQKDELAFKNKNITDSIN
                     YAKRIQESMLPSISRFKEFVPESFVIFKPKDIVSGDFYWVNETRNKTFIAVVDCTGHG
                     VPGAFMSIIGIELLRNITNVEGVDDAAEILNRLSVAIHNTFATSSFDSAEGVKVKDGM
                     DVSFCVIDKEYNMLQFAGAFSNLFLVRDGKLMEIKGDRYSVGMANELGHSQFSSYYIP
                     IQPNDTIYMFTDGIVDQFGGPEGKKFKYRRFRHLLLSNYMKPIELQRTNIEKAINDWM
                     GNLEQVDDMLIIGIKPELSCMF"
     gene            complement(5367..7256)
                     /locus_tag="PK049_05230"
     CDS             complement(5367..7256)
                     /locus_tag="PK049_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HOK60992.1"
                     /translation="MAYKSLSHFIDVLEQNGELLRVKRFVDPVLEITEITDRHSKLPG
                     GGKALLFENTGTNFPVLTNALGSERRICLALGTNNLEALRDDVGRLLEQFTRPRGSLW
                     SKISALPELTRVASWMPASRTGKGICQQVVHYNPHLDMLPVLKCWPHDGGRFITLPMV
                     HTVDPNTGTRNVGMYRMQIFDSSHTGMHWHKHKTGAKHFEEYKRLGKLMPIAVTLGGD
                     PAYTYAATAPMPENVDEYILAGFLRKKRVKLVKCLTVDIEVPEDVDFVIEGYVDPSEE
                     PAWEGPFGDHTGFYSLADWYPRLKVTCITHRADAVYPATIVGVPPMEDAYIAKATERI
                     FLEPIRRTLLPELTDMVLPTEGVAHNIALVKIKDEFQGQAYRVMNALWGAGQMMFNKV
                     LVAFPNNINLTDGYQMLNHICRTVNPQSDLFFGKGPLDVLDHSSNQFAFGSKLFIDAT
                     ALNYRETGYSISIDDIKSSTLAFSNHIVALNLTLIEKDIPILIASVRKKEIEDWQLFK
                     QELTLQPIFQDLFALVLVDLENQIDDLSLVLWFLSGNIDPSRDLSITRVNGSNLILID
                     GTRKAYPTDNFPRLWPNIVTMDGETIERVDQMWHWLDIGEFIESPSLKFFNLNQTDDA
                     VMRIQ"
     gene            complement(7338..8630)
                     /gene="eno"
                     /locus_tag="PK049_05235"
     CDS             complement(7338..8630)
                     /gene="eno"
                     /locus_tag="PK049_05235"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOK60993.1"
                     /translation="MGQIVSVHAREILDSRGNPTVEVDVITESGYFGRAAVPSGASTG
                     VHEAVELRDGDKGRYLGKGVLKAVNNVNTIIADEIVGMNVLDQQLIDETMIKLDGTPN
                     KGNLGANAILGVSLAVAHAAAQLTNQPLYRYVGGVNARTLPIPMMNILNGGSHADNSI
                     DFQEFMIMPVGANSFTEAIRMGAEVFHNLKSVLKKLGYSTNVGDEGGFAPNLKSNEEA
                     ITVILQAIENAGYRPGEDIYIALDPASSEYYLPEEKVYHLHKSTGEKLTPAQMVDYWK
                     NWVDKYPIISIEDGMAEDDWDGWKLITQTLGNRIQLVGDDLFVTNVNRLQKGIDTGVG
                     NSILIKVNQIGTLTETINAVRLADVHSMTSIMSHRSGETEDTTIAHLAVALNTGLIKT
                     GSASRSDRIAKYNQLIRIEEMLGDAAIYPGKNFKYIKK"
     gene            complement(8716..9240)
                     /gene="rplQ"
                     /locus_tag="PK049_05240"
     CDS             complement(8716..9240)
                     /gene="rplQ"
                     /locus_tag="PK049_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOK60994.1"
                     /translation="MRHNKKFNHLGRKSAHRKAMLSNMASSLILHKRITTTVAKAKAL
                     RSYVEPLITRSKDDSTHQRRVVFSHLQNKQAVTELFREVAQKIGDRPGGYTRILKTGT
                     RIGDAADMCIIELVDFNTTYVKETAKPAAKKTRRAGSKAKKAETGSANVVAEEPKSEN
                     QDSENATSTDEKAE"
     gene            complement(9246..10238)
                     /locus_tag="PK049_05245"
     CDS             complement(9246..10238)
                     /locus_tag="PK049_05245"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOK60995.1"
                     /translation="MAILAFQKPDKVIMLESTDNFGKFEFRPLEPGYGITVGNALRRI
                     LLSSLEGYAITWIKIRGVDHEFSTIPGVMEDVTEIVLNLKQVRFKRIVDGVDNEKVTI
                     TITGQNEFKAGYLSNFLSGFKVLNPELVICRMEPDVKIEIEVNISKGRGYVPAEENKP
                     EDAEFGIIPIDSIHTPIRNVKYSIENYRVEQKTDYEKLIIEISTDGSIHPKDALKEAA
                     KILIYHFMLFSDEKITLETEDKFETEEFDEEILHMRQLLKQKLVDLDLSVRALNCLKA
                     ADVETLGELVRYNKNDLLKFRNLGKKSLAELEDLLDSLNLSFGMDVSKYKLDKD"
     gene            complement(10254..10862)
                     /gene="rpsD"
                     /locus_tag="PK049_05250"
     CDS             complement(10254..10862)
                     /gene="rpsD"
                     /locus_tag="PK049_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOK60996.1"
                     /translation="MARYTGPTTKIARKFGEPIYGPDKYFEKKNYPPGVHGLMKKRKK
                     VSEYGTQLLEKQKAKYTYGLLERQFANLFKQAARSKGITGEILLQLLESRLDNVVYRL
                     GIAPSRAAARQLVLHKHIVVNGKVVNVPSFRLRPGDVVGVRERSKTLEAITDSLQSNR
                     HIKYSWLEWNNDKMEGKFMNVPERSEIPENIKEQLIVELYSK"
     gene            complement(10897..11283)
                     /gene="rpsK"
                     /locus_tag="PK049_05255"
     CDS             complement(10897..11283)
                     /gene="rpsK"
                     /locus_tag="PK049_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOK60997.1"
                     /translation="MAKKTATKKKVVKVDPVGQAHIHSSFNNIIVTMTNSTGQVISWS
                     SAGKMGFKGSKKNTPYAGQTAAADCAKVAYDMGLRKVKVFVKGPGAGRESAIRTINST
                     GIEVTEIVDVTPLPHNGCRPPKRRRV"
     gene            complement(11294..11671)
                     /gene="rpsM"
                     /locus_tag="PK049_05260"
     CDS             complement(11294..11671)
                     /gene="rpsM"
                     /locus_tag="PK049_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOK60998.1"
                     /translation="MARIVGVDLPKNKRGEVALTYIYGIGRSSARKILDKAGISYDTK
                     VADWTDDNVTAIRNILNEGYKVEGELRSMVQLSIKRLMDIGCYRGIRHRLGLPVRGQS
                     TKNNARTRKGKRKTVANKKKATK"
     gene            complement(11709..11825)
                     /gene="ykgO"
                     /locus_tag="PK049_05265"
     CDS             complement(11709..11825)
                     /gene="ykgO"
                     /locus_tag="PK049_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HOK60999.1"
                     /translation="MKVRASVKKRSDECKIVRRKGRLYVINKKNPKFKQRQG"
     gene            complement(11845..12063)
                     /gene="infA"
                     /locus_tag="PK049_05270"
     CDS             complement(11845..12063)
                     /gene="infA"
                     /locus_tag="PK049_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOK61000.1"
                     /translation="MSKQTAIEKDGTIIEALSNAMFRVELDNGHVITAHISGKMRMHY
                     IKILPGDKVRVEMSPYDLTKGRITFRYK"
     gene            complement(12078..12854)
                     /gene="map"
                     /locus_tag="PK049_05275"
     CDS             complement(12078..12854)
                     /gene="map"
                     /locus_tag="PK049_05275"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOK61001.1"
                     /translation="MFLAKTDEEVELLRLNNDLVSRTLAEVAKHIKPGVTTQKLDSIA
                     EEFIRAHGAVPGFLGYNGYPATLCISVNDAVVHGIPSSYELREGDIVSVDCGTFLHGF
                     YGDSAFTFSVGEVKPEVQRLLKVTRECLELGIEAAVAGGRVGDISYAVQSHAESNGYS
                     VVRELVGHGIGRHMHEKPEVPNYGARGRGPKLVDGMAICIEPMINLGSKHVYQDLDGW
                     TIRTRDGKPSAHFELAVVVRKDKAERLSTFKYIDEVLRLS"
     gene            complement(12884..14200)
                     /gene="secY"
                     /locus_tag="PK049_05280"
     CDS             complement(12884..14200)
                     /gene="secY"
                     /locus_tag="PK049_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOK61002.1"
                     /translation="MKAFFETLKNIYKIEDLRKRILYTLGILLIYRLGSFIVIPGLDP
                     SQLGALQEQTKEGIMGLLDMFSGGAFHNASIFALGIMPYISASIVVQLLGIAVPYFQR
                     LQKEGESGRNKLNQITRVLTVGILLFQGPAYITNLYIQLPQGAFLLSKFWFTITATIT
                     MIGGTMFIMWLGEKITDKGIGNGISLIIMIGIIARLPFAFVAELFSKAEELGGGLVML
                     LIELVILFAVFMFSILLIQGTRKIPVQYAKRIVGNKQYGGVRQYIPLKVNAAGVMPII
                     FAQALMFIPMLFARFDATAGIAAAFSYTGFLYNFTFGLLIIVFTYFYTAIIINPNQMA
                     EDMKKNGGFIPGVKPGKKTVEYLDSIMSKITLPGSIFLAIIAILPAFAIKLGVNNQFA
                     QFFGGTSLLILVGVVLDTLQQIESHLLMRHYDGLMKSGRLKGRSGM"
     gene            complement(14207..14653)
                     /gene="rplO"
                     /locus_tag="PK049_05285"
     CDS             complement(14207..14653)
                     /gene="rplO"
                     /locus_tag="PK049_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010956441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOK61003.1"
                     /translation="MKLDKLKPAEGSTHNRKRIARGEGSGHGGTATRGHKGAKSRSGY
                     SRKFGFEGGQMPIQRRLPKFGFKNPFRVEYKPINLEVLQALAEKHGYTTIDIDTLINA
                     GLVSRNDMVKILAKGTLSAKLEVKAHAFSKKAIEAIEAANGTAVKL"
     gene            complement(14680..14859)
                     /gene="rpmD"
                     /locus_tag="PK049_05290"
     CDS             complement(14680..14859)
                     /gene="rpmD"
                     /locus_tag="PK049_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009336598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOK61004.1"
                     /translation="MAKIKVTQIRSVIGRPEGQRLTLRSLGIRRMHHTVEVEATPQVM
                     GMIAKVRHLVRIEEQ"
     gene            complement(14872..15393)
                     /gene="rpsE"
                     /locus_tag="PK049_05295"
     CDS             complement(14872..15393)
                     /gene="rpsE"
                     /locus_tag="PK049_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOK61005.1"
                     /translation="MSQNTNIRRVKASDLELKDRLVSIQRVTKVTKGGRTFSFSAIVV
                     VGNENGVVGYGLGKASEVTAAIAKGIEDAKKNLVKVPILKGTIPHEQEAKFGGAKVLI
                     KPASHGTGVKAGGAMRAVLESVGVTDVLAKSKGSSNPHNLVKATVMALLELRDAHTVA
                     QQRGVDLKKVFNG"
     gene            complement(15399..15758)
                     /gene="rplR"
                     /locus_tag="PK049_05300"
     CDS             complement(15399..15758)
                     /gene="rplR"
                     /locus_tag="PK049_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004380845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOK61006.1"
                     /translation="MALNKLERKLRIRRRIRKRVSGTAQKPRMSVFRSNTNIYVQFID
                     DINGVTLASASSMSKEIAEKKNITKTEQARLVGQLAAKNAMAKGIESVVFDRGGNLYH
                     GRVKALADAAREGGLKF"
     gene            complement(15781..16335)
                     /gene="rplF"
                     /locus_tag="PK049_05305"
     CDS             complement(15781..16335)
                     /gene="rplF"
                     /locus_tag="PK049_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOK61007.1"
                     /translation="MSRIGKKPVNLPQGVSVNISADNVVSVKGPLGELKQKVDPDITV
                     KVEGNEVVLSRPTDHKRHRSLHGLYRALINNMVIGVSKGFEVQQELVGVGYKAEAKGQ
                     ILEMSLGFSHDIYFEMPKEIKVETITEKKGNPKIILKSIDKQLLGQVAAKIRSLRKPE
                     PYKGKGIRFVGEVVRKKAGKSASV"
     gene            complement(16357..16752)
                     /gene="rpsH"
                     /locus_tag="PK049_05310"
     CDS             complement(16357..16752)
                     /gene="rpsH"
                     /locus_tag="PK049_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOK61008.1"
                     /translation="MTDPIADYLTRVRNAVMARHRVVEVPASNMKKDITKILFDKGYI
                     LNYKFEDDGKQGIIKIALKYHPETKACAIKHLERVSTPGLRRYSNAKDLPRVLNGLGI
                     AIISTSQGLMTDKEARAKNIGGEVVCYVY"
     gene            complement(16778..17047)
                     /gene="rpsN"
                     /locus_tag="PK049_05315"
     CDS             complement(16778..17047)
                     /gene="rpsN"
                     /locus_tag="PK049_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004296337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HOK61009.1"
                     /translation="MAKESMKAREVRRAKLVAKYAEKRARLKEEGDYVGLQKLPRNSN
                     PIRLRNRCMLTGRPRGYMRQFGISRITFREMASKGLIPGVKKASW"
     gene            complement(17051..17608)
                     /gene="rplE"
                     /locus_tag="PK049_05320"
     CDS             complement(17051..17608)
                     /gene="rplE"
                     /locus_tag="PK049_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOK61010.1"
                     /translation="MEYVPTLKKKYREEIVPALMKQFNYKSVMQVPRLEKIVINQGIG
                     QAVADRKLIEQAQAELTMIAGQKAIQTYSRKDISNFKLRKGVPIGLKVTLRRERMYEF
                     LERLINVSLPRIRDFKGINDKFDGRGNYTLGIQEQIIFPEIDIDKVSKILGVEITFVT
                     TSENDEEAYALLREFGLPFKNAKKE"
     gene            complement(17608..17928)
                     /gene="rplX"
                     /locus_tag="PK049_05325"
     CDS             complement(17608..17928)
                     /gene="rplX"
                     /locus_tag="PK049_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOK61011.1"
                     /translation="MAKLHIKKGDMVYVISGESKGHQGRVLSVLVKENRAIVEGANMV
                     SRHTKPNSKNPQGGIIKKEAPIHISNLMLVDPATGKPTRIGRRLNDQGKLVRYSKKSG
                     EEIK"
     gene            complement(17949..18314)
                     /gene="rplN"
                     /locus_tag="PK049_05330"
     CDS             complement(17949..18314)
                     /gene="rplN"
                     /locus_tag="PK049_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOK61012.1"
                     /translation="MIQQESRLVVADNSGAKEVLCIRVLGGTGKRYARIGDKIVVTVK
                     SAIPGGDIKKGTVSKAVVVRTKKEIRRQDGSYIRFDDNACVLLNNQDEIRGTRIFGPV
                     ARELRDNYMKIVSLAPEVL"
     gene            complement(18317..18589)
                     /gene="rpsQ"
                     /locus_tag="PK049_05335"
     CDS             complement(18317..18589)
                     /gene="rpsQ"
                     /locus_tag="PK049_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOK61013.1"
                     /translation="MEQVNEVKRNNRKESIGVVVSNKMQKSIVVAVKRKVKHPIYGKF
                     VNKTTKFYAHDENNVCEVGDLVRIMETRPLSKLKRWRLVEIIEKAK"
     gene            complement(18602..18802)
                     /gene="rpmC"
                     /locus_tag="PK049_05340"
     CDS             complement(18602..18802)
                     /gene="rpmC"
                     /locus_tag="PK049_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOK61014.1"
                     /translation="MKVAEIRELTDKEILERIELEKTNLLKLRLNHSISPLDNPMKIK
                     ETRRNIARLQTVLNQRKLSQNK"
     gene            complement(18810..19238)
                     /gene="rplP"
                     /locus_tag="PK049_05345"
     CDS             complement(18810..19238)
                     /gene="rplP"
                     /locus_tag="PK049_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019152134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOK61015.1"
                     /translation="MLQPKKTKYRRQQKGKMKGIAQRGNQLAFGSFGIKALESSWITS
                     RQIEAARQAVTRYMKREGQIWIRIFPDKPITKKPAEVRMGKGKGAPEGFVAPVTPGRI
                     LFECDGVPYEVAKEALRLAAQKLPITTKFVVRPDFVEPTN"
     gene            complement(19270..20007)
                     /gene="rpsC"
                     /locus_tag="PK049_05350"
     CDS             complement(19270..20007)
                     /gene="rpsC"
                     /locus_tag="PK049_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOK61016.1"
                     /translation="MGQKVNPIANRLGIIRGWDSNWYGGKKYAQKLVEDSKIRDYLNA
                     RLAKASLSKIVIERTLKLITVTIHTARPGIIIGKGGQEVDKLREELRKITNKDVQINI
                     FEVKRPEIDAVIVGNNIARQIEGRISYRRAIKTAIASTMRMGAEGIKVQISGRLGGAE
                     MARSETYKEGRIPLHTFRADIDYALAEAHTKVGVIGIKTWICNGIVYGKRDLSPNIGT
                     KSVANVTEKKAKPAPKGGSRPRAKKKK"
     gene            complement(20015..20428)
                     /gene="rplV"
                     /locus_tag="PK049_05355"
     CDS             complement(20015..20428)
                     /gene="rplV"
                     /locus_tag="PK049_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007174500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOK61017.1"
                     /translation="MGARKHNMAERLKAERKNKAVARLRNCPSSPRKMRLVVDLVRGK
                     DVNTALNILKFSRKDAAKDVHKLILSAVANWKAKNENVRIEDANLYIKEIFVDQGRML
                     KRIRTAPQGRAHRIRKRSNHVTVVVDSAVNNESNN"
     gene            complement(20452..20721)
                     /gene="rpsS"
                     /locus_tag="PK049_05360"
     CDS             complement(20452..20721)
                     /gene="rpsS"
                     /locus_tag="PK049_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOK61018.1"
                     /translation="MSRSLKKGPFIDYKLEKRVIQMNESNKKSVIKTWSRASMISPDF
                     VGHTIAVHNGNKFIPVYITENMVGHKLGEFAPTRTFRGHAGNKNK"
     gene            complement(20741..21565)
                     /gene="rplB"
                     /locus_tag="PK049_05365"
     CDS             complement(20741..21565)
                     /gene="rplB"
                     /locus_tag="PK049_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOK61019.1"
                     /translation="MAVRKLKPVTPGQRHKIIGLFDDITTNKPEKSLLVPLKKTGGRN
                     NQGRRTMRYIGGGHKRRYRLIDFLRNRENEPAVVKTIEYDPNRTARIALVEYPDGEKR
                     YIVAPAGLKVGQTIMSGRGVAPEVGNTLYLGEIPLGTIIHNIELYPGRGACMARSAGS
                     YAQLLAREGKYAIIKLPSGETRMVLNECKATIGSVSNSDHALEKSGKAGRSRWLGRRP
                     RTRGVAMNPVDHPMGGGEGRASGGHPRSRKGIPAKGYKTRSKKKHSSKFIIERRKK"
     gene            complement(21571..21861)
                     /gene="rplW"
                     /locus_tag="PK049_05370"
     CDS             complement(21571..21861)
                     /gene="rplW"
                     /locus_tag="PK049_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOK61020.1"
                     /translation="MDILIKPVVTEKMERLTDKLNQYGFIVDKRANKLQIKKAIEELY
                     GVTVDSVNTMRYAGKVKSRYTRTGVLVGRTNSYKKAIVTLKEGDKIDFYSNI"
     gene            complement(21874..22506)
                     /gene="rplD"
                     /locus_tag="PK049_05375"
     CDS             complement(21874..22506)
                     /gene="rplD"
                     /locus_tag="PK049_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOK61021.1"
                     /translation="MEIKVLNIQGKETGRTIQLEDSIFGIEPNDHAIYLDVKQILANR
                     RQGTHKSKQRNEVSGSTRKLKRQKGTDTARAGSIKSPVFRGGGRIFGPVPRDYSFKLN
                     KKIKQLARKSALAYKVKENALLVIEDFSFDTPKTKEFVNICKNLNVADKKTLFLLGET
                     NKNIYLSSRNLQKQATAIASSVNTYEILNANMLVLTESSVDALNKMFKVS"
     gene            complement(22506..23123)
                     /gene="rplC"
                     /locus_tag="PK049_05380"
     CDS             complement(22506..23123)
                     /gene="rplC"
                     /locus_tag="PK049_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOK61022.1"
                     /translation="MPQGLIGRKIGMTSVFGEDGKNIPCTVIEAGPCVVTQVKNAETD
                     GYAAIQLAYDDKKEKHTSRPMLGHFKKANTTPKRKLVEFRDWEGLQLGDVVTVDLFAN
                     DNWVDVVGITKGKGFQGVVKRHGFNGVGGRTHGQHNRQRHPGSMGASSFPSRVLPGKR
                     LAGRTGGDRVKIINLRVLKVIPESNLLLVKGSIPGAKGSYIIIEK"
     gene            complement(23149..23454)
                     /gene="rpsJ"
                     /locus_tag="PK049_05385"
     CDS             complement(23149..23454)
                     /gene="rpsJ"
                     /locus_tag="PK049_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOK61023.1"
                     /translation="MSQKIRIKLKSYDHNLVDKSAEKIVKTVKATGAVVSGPIPLPTH
                     KRIFTVNRSTFVNKKSREQFELSSYKRLLDIYSSNAKTIDALMKLELPSGVEVEIKV"
     gene            complement(23477..25594)
                     /gene="fusA"
                     /locus_tag="PK049_05390"
     CDS             complement(23477..25594)
                     /gene="fusA"
                     /locus_tag="PK049_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOK61024.1"
                     /translation="MSRDLRFTRNIGIMAHIDAGKTTTTERILFYTGKTHRIGEVHDG
                     AATMDWMVQEQERGITITSAATTTFWNYENKQYQINIIDTPGHVDFTVEVERSLRVLD
                     GAIATFCAVGGVEPQSETVWRQADKYRVPRIAYVNKMDRVGADFLGVVQEIKDKLGAN
                     PIPIQLPIGAEDNFNGIIDLVENKAVVFFEDEKLKATNYRLEPVPANMKDEVDEWRAK
                     LIEAVASYNDELLERFFEDPDSITKQEIIETIRKATIDMSIIPVLCGSSFKNKGIQHL
                     LDAVIAFLPSPLDKGDVVGTNPNTGNEEVRKPYIDQPFCGLAFKIATDPFVGRLAFIR
                     VYSGKVDSGSYVFNTRTGKKERISRIYQMHANKQNPIEFCEAGDICAAVGFKDIRTGD
                     TLCLEEKPIALEAMTFPEPVIGLAIEPKTQKDLDKLGIALGKLSEEDPTFRVNTDPDS
                     GQTVISGMGELHLEIIVDRLRREFNVEINQGAPQVAYREAFTRSVSHREVFKKQTGGK
                     GKFADIIFELGPADEGVTGLQFIDEVKGGNIPREFIPSVQKGFELAMSNGVLAGYAIP
                     GMKVVLKDGSFHPVDSDALSFEIAARYGFRAAGPKAAPVLLEPIMNVEVVTPEENMGD
                     VIGDLNKRRGQIAGMESKGNACVVKAKVPLAEMFGYVTVLRTLTSGRATSTMEFSNYA
                     EVPSNIAKEVIEKSKGKELKEIE"
     gene            complement(25626..26102)
                     /gene="rpsG"
                     /locus_tag="PK049_05395"
     CDS             complement(25626..26102)
                     /gene="rpsG"
                     /locus_tag="PK049_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOK61025.1"
                     /translation="MRKAKPKKRIILPDPKFGDIMVTKFVNNLMVTGKKSIAFGIFYN
                     ALDIVGKRMKDSEKSPLEIWKIALENVTPQVEVKSRRVGGATFQVPTEVRPERKISLS
                     MKNLILFARKRVGKGMSDKLAAEIMAAYNQEGGAFKRKEDMHKMAEANKAFAHFRF"
     gene            complement(26132..26521)
                     /gene="rpsL"
                     /locus_tag="PK049_05400"
     CDS             complement(26132..26521)
                     /gene="rpsL"
                     /locus_tag="PK049_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOK61026.1"
                     /translation="MPTIQQLVRKGRSKLVDKSKAPALDACPQRRGVCVRVYTTTPKK
                     PNSAMRKVARVRLTNGKEVNAYIPGEGHNLQEHSIVLIRGGRVKDLPGVRYHLIRGTL
                     DASGVEGRKQGRSKYGAKRPKDKASKK"
     gene            26925..29081
                     /locus_tag="PK049_05405"
     CDS             26925..29081
                     /locus_tag="PK049_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOK61027.1"
                     /translation="MKTYQTEQIRNIALLGNTGSGKTSLAESMMFEGKIIERKGTIDG
                     KNTVSDYTEIEQQNQRSIFSTLLYTEFNNSKLNIIDTPGSDDFVGAVISSMYPTDLLV
                     MVINAQYGVEVGTEIFNRHAEHLHKPMILAVNQLDHDKANFDATLDSLKQTFGNKVIL
                     TQYPVATGAAFDSFIDVITMKMYKFKGDTGIREEFPIPDSEMDKATELHNALIEAAAE
                     NDESLMEKFFEQGSLSEDEMRSGLSKGIASRSIMPVFCLSAAKNIGTKRLMDFIINVG
                     PNPTHSPVLKNNSDKPITCDTSAPTSLFIFKSTVEPHIGEISYFRVMSGKVTEGMDLT
                     NANNSTKERLSQLFVVAGKNRNKVTELVAGDIGATVKLKNSKTNHTLCGPGLDFTFDP
                     MEFPDPKFRTAVKPKNEAEVEKLGELLNRAHQEDPTILVEHSKELRQIILSGQGEFHL
                     NILKWQLTNLHKLEIEYIAPRIPYRETITKVAQADYRHKKQSGGAGQFGEVHLVIEPY
                     VEGAPDPVKYKVNGKEINLSVRGKEVIDLEWGGKLVFYNCIVGGAIDARFMPAILKGI
                     MEKMEQGPLTGSYARDIRVAVYDGKMHPVDSNEISFKLAGRNAFKNAFKDAGPKIMEP
                     IYDVEVLVPADRMGDVMSDLQNRRAIIMGMGSVKGFEVINAKVPLAELNRYSTALSSL
                     TSGRATYTMKFASYEQVPADVQEKLLKAYQEQEEEE"
     gene            29163..>29616
                     /locus_tag="PK049_05410"
     CDS             29163..>29616
                     /locus_tag="PK049_05410"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOK61028.1"
                     /translation="MRTAEIHTSKGVMKVQFYEEDAPNTVANFVKLAKEGFYDGLAFH
                     RVIPNFVIQGGCPYSKDMTDPRVGTGGPGYKINCELTGNNQYHDRGVLSMAHAGRNTG
                     GSQFFICHNRQNTKHLDRQHTCFGKVYEGLEVIDAIRVGDRIEKIVIVE"
BASE COUNT         7694 a         7124 c         6429 g         8369 t
ORIGIN      
        1 ttggtgaggg tgaatttaat ctcagtaaat cgcttgaaac ccttggggta ccagttgtaa
       61 acctgaccga gaatgtccct gactgggcaa aacacagcta tgtttacata caggatacct
      121 ttgcaattaa tctaaatgag gtttacaatc agtccgatct gattagctac ataatgattc
      181 gcgttaacat ttggaatgag tttccactac aaggaaatgc ccaaatatgg atacttgaca
      241 atggccaaaa taagttagac tcactgtttg cacaaccggc cagtgttcct gcagccgaga
      301 tatactcaaa tggcaacgtg gtgggcacca agtttgaatc gtataaggtt gattttgacc
      361 aaaatcgtgt cgacttatgg caaaacgcgg ccacagcagt ggttagggcc ggattggtaa
      421 ttactgaaga aggtgttact gagcagaata tccaatattt tgaccgttac aagctaaaag
      481 tacagctggc tgcacgtgtc gatttcaaaa ctatcaaaaa taattagggt atgttactgc
      541 gatttaccac tctctccgct ttattttttg cttatacatt ggcatgggca cagcaaagca
      601 actcgctttt ctttatgcat cgcataccgc aatcgaacct aataaaccca gcagttcaga
      661 ttgattgtcc ggtatatgta ggtgtgcctt tcctttcgtc gttacaccta aacctgaact
      721 ctaccggttt ttcctataat gatattgcaa acggaacagg taccattaac tttcctgccc
      781 ttgttgggca aatgcatagc tgggactacc tatcggagga actccactac acacccgttt
      841 cgttcggatt tatgtacgac aacgaccagt actttaactt tgcctggact gagcgagtgg
      901 agagtaagct atttgtatcg aaacgtttga tgtcgctttt tgctaacggg aatacgcagt
      961 tcgtgggcaa tgggctaata acctccaatc ccggcctaaa cagcttttac tacagggagt
     1021 tttcatttgg ctactccacc cggttacgtg gcgatgtgct tgttggtgcc catgccaaaa
     1081 ttctgttcgg tcttgcaggc ctttacacca aaaggaatag cgttgatatt gagatagatg
     1141 ccttaaccca caatattaat gcccgttgga atccacaggt aaacgtagcc taccccttag
     1201 gggtaactac cgatgctgca gggaatgtta ccggagttag cgcaggggca tttagcccat
     1261 tgccattctt ccttaacttc agcaatccgg ggcttgcatt cgacttgggc tttgtaaagc
     1321 ccaacgaccc tattacatgg agtggtagca ttctggactt aggatttata tggtggttca
     1381 aaagcacctc gcgctttacc aacgatggcc attttgttta ccgtggtgcc acccctgccg
     1441 accttaccaa ccccgatgcc tatatcgaca tgcttgccga ttccataaac aatcaggttc
     1501 aattccagca ctcaaacggt agctttgtgt catttttaaa ccctaaagcc tattttggag
     1561 gcacttacgc atttacccca caactacttg cgggtgcaca tgtaagaacc gagtggtatc
     1621 cgggcaggcc tgtggtagga ctgaccctat cggccattgc tatggccaag aaaggttcat
     1681 cggtttcatt aacctactct gtgatgaacg gctcttttgc caacattgga gccgctatta
     1741 accttggggg tgaaagcttt cagttttaca ttcttaccga taacctgctt gccccattct
     1801 acccggaaag ggtacgaaat gcaaacctaa ggtttggctt taacctgttc tggggttgta
     1861 agcaaaaaaa gaaaaagtta agcattccgg aatcgagcgg tggtggttgt ttctggacat
     1921 ggggtttgga ccagaaacga aagcagcatg gggtaaagta aaagagtaaa tagaactgga
     1981 attttaggca ttatcaccag taaacaatac tagaacatac agcttaactc tggtttaata
     2041 cctataatta gcatatcgtc aacctgctca aggttaccca tccaatcgtt aatggctttt
     2101 tcaatattag tcctttgcaa ttcgataggc ttcatatagt tactcaaaag aaggtgtcta
     2161 aacctgcggt atttgaactt cttaccctcc gggcctccaa actgatcgac aattccatcg
     2221 gtaaacatgt aaattgtgtc gtttggttgg attggtatgt agtagctgct gaactgactg
     2281 tgccctagct catttgccat acccacggag tacctgtcgc ccttgatctc cattagcttt
     2341 ccatcgcgaa ccaggaaaag gttagaaaag gcgccagcaa actgcaacat gttatactcc
     2401 ttgtcgataa cacagaacga tacatccata ccatccttaa cttttacacc ctcagcacta
     2461 tcaaaactac tggtagcaaa tgtgttatga atggccacgc taagcctgtt aagtatttcg
     2521 gcagcatcgt caacgccttc aacgttggtg atatttctaa gcaactcaat accgatgatt
     2581 gacatgaacg ccccaggaac gccgtgacca gtacagtcga ccacagcaat aaaggttttg
     2641 ttacgggttt cgttcaccca gtagaaatca ccgctaacaa tatccttagg tttaaaaatc
     2701 acaaacgatt caggtacaaa ttccttaaat cggctaatgg aaggaagcat cgattcctga
     2761 atacgttttg cgtagttaat actatcggtg atattcttat tcttaaaggc caactcgtct
     2821 ttctgcaacc taagttcctc aagcacagtt tcacgttcat ggagtaagcg gttaatttca
     2881 cgcaaatgct tatttctacg aatggcccat actaagagag aaatcagtac aataacccca
     2941 taaatccact tggcaatccg cgatcgccac caaggcgcaa cagcacgaat ccttagcgat
     3001 ttatattctg ttgtccaaac ctgatcgcta ttggaaccca taattcttaa ctcgtagtcg
     3061 ccttcagaaa ggtttgaaaa agtaattttg ttagtatttc ccagctcaac ccaacggcta
     3121 tcgtgaccaa caagctgata gcggtacgaa ttcttgttgg gttgaacaaa atcaagggct
     3181 gcaattgtga aggttaagtt gctaaaatca tcaggtatcc ttaggaactt aagctgatac
     3241 gggattattt cggtgttgcc cgacttactg tacacctgaa tgtgagtaat acaaacattt
     3301 ggaacaacct tattggtagg aattgaatcg ggatggaata cgttaacacc gtcgatactc
     3361 ccaaagtaca tcctcccttt actattctta aacgatgcgt ttatgtttag ctcattgcta
     3421 aaaagcccat cggagttatt gaaaacctta accaaattgg aaagaggatt atacagaacc
     3481 aatcccttat tggtgctaat ccagaatcgg ccacgatcat cctccagaat atcgtttata
     3541 agcctgatat taatatcggg tagtacaacc gaatgcagaa cattttttcc ttttgggtta
     3601 atccagaata aaccactggt agtaccaacc caaactgttt cgttcttggc aatgtaaagg
     3661 caaagcactt cgaaaggcaa cctactttta ttgccaatga atgagaatgt ttcaatggtt
     3721 ctcaaatcgg ctgatattct gcaaagtcct gaaattgttg ccacccaaac gttagcattt
     3781 ttgtcggcaa ccacatcgta aacctcattt cccggtaacg aaagggaatc accccttctg
     3841 gcatagaaac ttatgagatt tccacctttc aagcaatgca atccctgccg ggtggcaaac
     3901 caaactctat cctgtaaatc ctcagttatg gagtaaaccc tgttgttctt aaacagatcg
     3961 ccaacataca cattgttcct ctcaaaaaag tcgacaaact gcctcgatga aggaagatat
     4021 tgaaaaaccc cattgcgtgt gcctacaagt accctaccat cgcgtgtttg gtaaagggaa
     4081 tgaataaaat cattaggaat gtagttgcct cctcccgacc agtaatgtcg gttttcacca
     4141 gttgtggggt tgaagatatg caaaccgcta ccccatgttc caacccaaat attaccgtca
     4201 gtgctttcaa ttaccgatgc tgcaacgttg tttgagaata ggttccgccc gtttggatcc
     4261 ttactgtacc caccaatacg gtgctcaaag gggtttacct taaccagccc ggtttgcgaa
     4321 ccaatccaca gtactcccgt cctatcgcga agcaccgttg atacatttaa aatatttacg
     4381 ggtaaattat tgctataaat gctattgtac ttaataaatg taccgtttgg aagtagttta
     4441 cccaatcctc ccacgctccc aacccaaagg gaggaatcgt tatcataacc caggaactga
     4501 attccagtag tcaacgattt gttggaaggg tcgttaagcg taaggattac tctacgaaat
     4561 ttctgtgtaa gaaagtcaaa ctcatttatc cccttatcgg taccaattag caatttcccg
     4621 cttaaaccct cagtaatagc attaacccta ttaccccaaa tggaatttgg attagctgga
     4681 tcttcggagt accttttaaa aactagacgt tctttatcga aatagaatag tccgtcctta
     4741 gtgccaaccc aaagtcgttc gtttgaattc tcaaaaatcg gcgacaaatc ggatgtttgt
     4801 gatatgttaa aaatatcgtt gaaatgcgga aatcgggtaa aatcgtcggt tgttgggttg
     4861 tataaatgta aggcatcaat ggtttttacc caaatcctat tactccggtc gcggtacacc
     4921 ctaagtatca tattggacga cagcgacctt gggttatttg catgattgta gtaaacctta
     4981 accttccctg tttttcggtt aaccctatta agcccactac gggttgctat ccaaagttca
     5041 ccatttacac cctcaaccaa atgatttacg tagttattgg agagcgaaag gctatcgtag
     5101 gggttgttgt taaaaaccac aaaatcgtaa ccatcgtaac ggcataaacc atcctgggtg
     5161 ccaatccaca ggaagcctaa ggaatcctgt aataatgcat ttacaaacga ttggggcaaa
     5221 ccattatcgg agttaaacag ggtaacattt accctttgag caaaaaccga tgatgttgca
     5281 gccagcaaaa gagctgcaac gcaactaggc agtaattttt tatctgttct aaacaacccc
     5341 atggtttagg ctaaggtaca aaaaagttat tgaattcgca ttactgcatc atcggtttgg
     5401 ttcagattaa agaacttaag cgatggcgat tcaatgaatt cacctatatc gagccaatgc
     5461 cacatttgat caaccctttc aatggtttcg ccatccatgg taacaatgtt tggccaaagc
     5521 cttggaaagt tatcggtagg ataggccttt cgggttccat caatcaaaat caagttactt
     5581 ccgttaaccc gtgtaattga taaatcgcgt gagggatcga tattgcccga caaaaaccag
     5641 agcaccagcg aaaggtcatc aatttgattt tccaaatcaa caagaactaa agcaaacaaa
     5701 tcctgaaaga taggctgtaa tgtgagttcc tgtttaaaca actgccaatc ctctatctcc
     5761 tttttcctta ccgaggctat tagaataggt atgtcctttt caataagtgt caggtttaag
     5821 gcaactatat gatttgaaaa tgctagggta cttgatttaa tatcgtcgat actaatactg
     5881 taaccagttt ctctataatt aagcgcagta gcatcgataa acagcttgct accaaaagca
     5941 aactgatttg atgaatggtc gagcacatcc agtgggcctt tcccgaaaaa caagtcggat
     6001 tgagggttta cagttctaca tatatggtta agcatttggt aaccatcggt aagattgatg
     6061 ttatttggga aagcaacaag caccttgttg aacatcattt gtcctgcacc ccaaagtgcg
     6121 ttcattacac ggtaagcctg tccctgaaac tcatccttta ttttaaccag ggcaatgtta
     6181 tgggcaaccc cttcggtagg cagtaccata tccgttagct caggcaggag ggttcggcgt
     6241 attggttcaa gaaagatacg ttcggttgcc ttggctatat aagcatcttc cattggtgga
     6301 acaccaacta tggtggcagg gtaaacggca tcggcgcgat gggtaatgca ggtcacctta
     6361 aggcgcgggt accaatcggc cagggagtaa aaaccggtgt gatccccaaa agggccctcc
     6421 catgccggtt cctcggaagg atcgacatag ccttcaatta caaagtcaac atcctcgggt
     6481 acttcaatat caaccgttag gcacttaacc agctttaccc gttttttacg taaaaaaccg
     6541 gcaagaatgt actcatccac attctcaggc attggggcag tagctgcata ggtataggcc
     6601 gggtcgccac caagagtgac tgcaatgggc attagcttgc ctaagcgctt gtactcctca
     6661 aaatgcttag cgccagtttt atgcttgtgc cagtgcatgc ctgtatgtga ggagtcgaaa
     6721 atttgcatac ggtacatccc aacattccga gttccagtat tgggatcaac ggtgtgtacc
     6781 atgggcaggg ttataaacct acccccatcg tgtggccaac atttaagaac gggcagcatg
     6841 tcaagatggg ggttgtaatg gacaacctgc tggcaaatgc ccttaccggt ccggcttgct
     6901 ggcatccacg atgccacccg ggtaagttcg ggtaatgccg agattttaga ccaaaggcta
     6961 cctcttggcc gtgtaaactg ttcaagcagc ctacccacat cgtcgcgcaa tgcctccaag
     7021 ttattggttc caagagccag gcatattctt ctctcggaac ccaaggcatt ggtaagcacg
     7081 ggaaaattag tgccagtatt ctcaaataga agagctttcc ctccaccagg tagctttgaa
     7141 tgcctatcgg taatttcggt tatttccaga acgggatcaa caaaacgttt caccctcagt
     7201 agttcaccgt tttgctcgag aacatcaata aaatgactaa gcgacttgta ggccatgccc
     7261 gaactattta gcccctaaaa ataaaaaaag gcttcagata atacttccga agcctttgaa
     7321 aagttacaaa aaccgaacta ttttttgatg tacttgaagt tcttacctgg gtaaatagct
     7381 gcatcgccaa gcatttcctc aatacgtata agctggttat acttagcaat cctatcggaa
     7441 cgtgaagcag atccggtttt gataagacca gtgtttaagg ccacagccag gtgggcaatg
     7501 gtagtatcct cggtttcacc tgagcggtgg ctcataattg aggtcatgga gtgaacgtcg
     7561 gctaaacgaa cggcattgat tgtctctgtc aatgtaccaa tctggttaac cttaataaga
     7621 atactgttac ctacgccggt atcgataccc ttctgcaagc ggttcacgtt ggttacgaaa
     7681 agatcatcgc ctacaagctg aatcctattg cctaaggttt gggtgataag cttccagcca
     7741 tcccagtcgt cctcggccat accatcctca atggaaatga ttgggtactt gtcaacccag
     7801 ttcttccagt aatcaaccat ttgagcaggg gttagcttct cgccagttga tttatgcagg
     7861 tggtaaacct tttcctcggg caggtagtac tcggacgaag cggggtcgag agcgatgtaa
     7921 atatcctcgc ccggacggta tccggcattt tcaatggcct gtagtataac ggtgatagcc
     7981 tcttcgttcg atttcaggtt aggagcaaat ccaccctcat cgccaacgtt ggttgagtat
     8041 ccaagctttt tgagaacaga tttcaggttg tggaaaacct cagcacccat acggatagcc
     8101 tcggtaaagg agttggcgcc aacgggcata atcataaact cctggaaatc gatactatta
     8161 tcggcgtgcg aacctccgtt taggatattc atcatgggaa taggaagggt acgagcatta
     8221 acgccaccca cgtaacggta aaggggctgg ttggtaagct gagctgcggc atgggcaaca
     8281 gctagtgaaa caccaagaat tgcattggcg ccaaggttac ccttatttgg ggtgccatcg
     8341 agcttaatca tggtttcgtc gataagttgc tggtcaagaa cattcatacc aacaatctca
     8401 tcggctataa ttgtgttcac attgttaacc gccttaagca cgcccttgcc aaggtaacga
     8461 cccttgtcgc catcgcgaag ctcaacggcc tcatgaacgc cggttgaagc accacttgga
     8521 acagcagcac ggccaaagta tccactctca gtaatcacat ccacctcaac ggttggattg
     8581 cccctggaat cgagaatctc acgggcatga acgcttacaa tttgtcccat agtttgttgt
     8641 agtttttagt aaagttagat aaaaaaaggg attaagccaa aagctcatcc ctaaatcagt
     8701 attgttaaac tggtgttact cagccttttc gtcagttgaa gtagcatttt cgctatcctg
     8761 attttccgat tttggttctt cagcaactac gttagcgcta ccggtttcag cctttttagc
     8821 ttttgagcca gccctacggg ttttcttggc tgcgggctta gctgtttcct tcacgtaggt
     8881 agtattgaag tcaaccagtt cgataataca catatcggca gcgtccccaa tacgggtacc
     8941 agttttaaga atacgggtat aaccgccggg gcgatcgcca attttctgag ccacctcacg
     9001 gaaaagttca gttactgcct gcttgttttg aagatggctg aaaactacac ggcgctgatg
     9061 tgtagaatcg tccttgctcc gggtaatcag tggctcaaca taagagcgta aagctttagc
     9121 ttttgctaca gtagtggtga tacgcttgtg cagtatcaac gacgatgcca tattcgatag
     9181 cattgcctta cggtgagcgc tcttacggcc taaatggtta aattttttgt tatgtctcat
     9241 cgtaattaat ccttatccaa tttatacttt gatacatcca tgccaaacga gaggttcagg
     9301 ctatcgagca agtcctcaag ttcggcgagc gattttttac ctaagttacg gaacttgagt
     9361 aggtcgttct tgttgtaacg aaccaactcg ccaagggttt ccacatcggc agccttgagg
     9421 cagttcaatg cgcgaactga caggtcaagg tcaaccagct tttgcttcag cagctggcgc
     9481 atgtggagga tttcctcatc gaattcctca gtttcaaact tatcctcggt ctccagggta
     9541 atcttttcat cggagaatag catgaagtgg taaatcagaa tcttagcagc ttccttcagt
     9601 gcatccttcg gatgaataga gccatcggta ctgatctcga tgataagctt ttcgtagtcg
     9661 gttttctgct ctacgcggta gttttcgatc gagtacttaa cattacggat aggagtgtga
     9721 atggagtcaa tgggtatgat accaaactca gcatcttcag gcttgttttc ctcagcggga
     9781 acatatcccc tacccttgct gatgttaacc tcaatctcaa tcttaacatc aggctccatg
     9841 cgacagataa ccagttcagg gttaagcacc ttgaatcctg aaaggaagtt ggacaggtag
     9901 ccagccttaa actcgttctg ccccgtaatt gttatggtaa ccttctcgtt gtcaacaccg
     9961 tcaactattc gtttaaagcg aacctgcttc aggttgagaa ctatctcggt tacgtcttcc
    10021 attacaccgg gtatagttga aaactcgtgg tcaacgccac gtattttaat ccaggtaatg
    10081 gcataacctt cgagcgaaga tagcagaata cgccttaacg catttcccac cgtgatacca
    10141 tagcctggct caagaggacg aaactcgaac ttaccgaagt tatcggtaga ttcaagcatg
    10201 ataaccttat cgggtttttg gaatgccagt attgccatat ggtacaggtt ttattactta
    10261 gagtaaagtt caacaattaa ttgctccttg atattctcag gtatttccga acgctcaggt
    10321 acgttcatga atttgccttc cattttatcg ttattccact ccagccatga gtacttgatg
    10381 tgacggttgg actgaaggga atcggtaata gcctcgagtg tttttgagcg ttcacgaacg
    10441 ccaacaacat caccgggacg taacctgaat gagggtacgt ttacaacctt accgtttaca
    10501 acaatgtgct tatgcaacac aagctgacgg gcagctgcac gtgaaggagc aatacccaaa
    10561 cggtatacaa cgttatcgag acgcgactcg agcagctgaa gcaaaatttc accggtgata
    10621 cccttgctgc gagcagcttg cttaaatagg ttagcaaact gacgctcaag taggccataa
    10681 gtgtacttag ctttttgttt ctcgagcagc tgggttccgt actccgaaac ctttttgcgt
    10741 ttcttcatta agccgtgaac cccgggagga tagttcttct tctcgaagta cttatccggc
    10801 ccatagatgg gctctccgaa tttacgggca atcttggttg ttggtccagt atatcttgcc
    10861 attattttgt aattttattg attcgtacta aaatggttat accctacgac gtttaggagg
    10921 gcggcagcca ttgtgtggta gtggagtaac atcaacaatc tcagttacct caataccggt
    10981 agagttgatg gtacggatag ccgattcacg gccggcgccg ggtcctttta caaacacttt
    11041 taccttcctt aagcccatat cgtaggctac tttagcacaa tcggctgctg cagtttgacc
    11101 tgcatacgga gtattcttct ttgaaccctt gaaacccatc ttaccagccg acgaccagga
    11161 aataacctga ccggtgctgt tggtcattgt aacaatgata ttgttaaacg atgaatgaat
    11221 atgggcttgt cctaccggat caaccttaac tacttttttc ttagttgctg ttttctttgc
    11281 catagcttaa ttactattta gtagccttct tcttgttggc aacagttttt ctctttcctt
    11341 tacgtgtacg agcgttattc ttggttgatt gacctctcac aggtaaacca agacgatgac
    11401 gaatacctct gtagcaacca atatccatca agcgcttaat gctgagctga accatggaac
    11461 gcagttcacc ttcaaccttg tacccttcgt tcaggatgtt acggatggct gttacattat
    11521 catcggtcca atcggctact ttggtatcgt agcttattcc ggctttgtcc agaatcttac
    11581 gggcgctgct gcggccaata ccataaatat aggtaagggc tacctcgcct ctcttgtttt
    11641 tcggcaaatc tacacctact atacgtgcca tattttcact taataattag tgtgcaaaat
    11701 taaacaaatt atccttgacg ttgcttgaac ttaggatttt ttttgttaat cacatacaag
    11761 cgtcccttac gacgaactat cttgcactcg tcgctgcgct tctttactga tgctcttact
    11821 ttcattgtat tgaaattttt tggtttactt gtacctaaaa gtaatacgtc ctttagttaa
    11881 atcgtaaggc gacatttcaa ccctcacctt atcgccgggt aaaattttga tgtaatgcat
    11941 acgcatttta ccggagatgt gggcggtaat aacatgtccg ttatcgagct ctaccctgaa
    12001 catggcgttc gatagggcct caataatagt tccgtctttc tcaattgcag tttgcttgga
    12061 cattcgctct taaaaattta acttaaacgt aaaacttcat caatatactt gaaagttgaa
    12121 agcctttcgg ctttatcctt tcgaacaaca acggccaact caaagtgcgc cgagggttta
    12181 ccgtcgcggg tacggatggt ccatccatcc agatcctggt aaacgtgctt tgagccaagg
    12241 ttaatcattg gctcaatgca gattgccatt ccatcgacaa gcttaggccc acggcctcgt
    12301 gctccgtagt tagggacttc gggtttttca tgcatatggc ggcctatgcc atgtcccacc
    12361 aattcgcgga ctacagagta accgttcgat tcggcatgcg attgcacggc gtaggatata
    12421 tcgccaaccc tgccaccggc tactgcagcc tcaatgccta gctcaaggca ttcgcgggta
    12481 acctttaaca gccgctgcac ttcgggctta acctcgccca ccgaaaaggt gaaggcggaa
    12541 tcgccataaa acccgtgcaa gaaggtcccg caatcgaccg atactatgtc gccctcgcgg
    12601 agctcatacg acgatggaat gccatgcacc actgcatcgt tgaccgaaat gcaaagcgtt
    12661 gctgggtagc cattataccc caaaaaaccc ggaacagccc catgagccct gatgaactcc
    12721 tcggctattg agtcgagctt ttgggtggtt acccctggct taatatgttt tgccacctcg
    12781 gctaatgtgc gcgataccag atcgttgttt agccttagca gctcaacttc ctcgtcggtt
    12841 ttagctaaaa acattataaa gaacgtttgg caaaattgcc ctattacata ccggaacggc
    12901 ccttcagcct tcccgatttc attaatccat cgtaatgacg catcagcagg tgactctcaa
    12961 tctgctgtag tgtatcaaga actacaccta ctaagattaa caacgaggtt ccgccaaaga
    13021 actgggcaaa ctggttgtta actcccagct taatggcaaa tgcaggaaga atagctataa
    13081 tagctaagaa gattgaaccg ggtagagtga ttttcgacat gatggagtcg agatactcaa
    13141 cggttttctt tccgggtttt actccgggaa taaaacctcc gttcttcttc atatcctcag
    13201 ccatctggtt gggattgata ataatagcgg tatagaaata ggtgaatact atgatcagca
    13261 atccaaaggt gaagttgtat aggaatccgg tgtagctaaa agcggcagca atgccagcag
    13321 tagcatcaaa gcgggcaaac agcatgggga taaacatcag agcctgagca aaaatgattg
    13381 gcattacacc ggcagcgttt accttaaggg gtatgtactg gcgaacacca ccatactgtt
    13441 tatttcctac aattcgtttg gcatactgca caggtatttt acgtgtaccc tgaataagca
    13501 ggattgagaa catgaatact gcaaatagta ttacaagctc aattaggagc ataaccaatc
    13561 caccaccaag ctcttcggcc tttgagaaaa gttctgcaac aaaggcaaag ggtaagcggg
    13621 caataatacc aatcatgatg attagcgata taccgttacc tataccctta tcggtaatct
    13681 tctcacccaa ccacatgatg aacatagtac caccaatcat ggtgatagtg gctgtaatgg
    13741 tgaaccagaa cttacttaac aggaatgctc cctgtggaag ctgaatgtaa aggttggtta
    13801 tgtaggctgg accctggaat agcaggatac ctactgtaag tactcgagta atctggttaa
    13861 gcttatttct accactctcg ccttctttct gtaacctctg gaagtagggt acagcaattc
    13921 ctaaaagctg aaccacgata gatgctgaga tgtagggcat aatacccaag gcaaaaatgg
    13981 aggcattatg gaatgcacca cccgaaaaca tgtcgaggag gcccatgata ccttccttgg
    14041 tttgctcttg cagagcgcca agctgcgatg ggtcaagacc cggtattact ataaagctac
    14101 caagtcgata aatcagcaga ataccaagag tatagagaat ccgcttgcgc agatcctcta
    14161 tcttgtatat attcttgagg gtttcgaaaa aggctttcat tattcactat agttttactg
    14221 cggttccgtt agctgcttca attgcttcaa ttgctttctt tgagaaagcg tgggctttca
    14281 cctcaagctt agcgctaaga gtacccttgg caagaatttt aaccatatcg ttgcgcgaca
    14341 ctaaaccggc attgataagg gtatcaatat cgatagtggt atagccgtgc ttttcggcaa
    14401 gggcttgcaa cacttccagg ttaatgggct tgtactcaac gcggaatggg tttttaaagc
    14461 caaactttgg tagacgacgc tgtataggca tctggccacc ctcaaagcca aacttgcggg
    14521 agtaacctga gcgtgacttg gcacccttgt gaccacgagt ggcagtgcct ccatgtcctg
    14581 acccctcacc acgggcaata cgtttcctgt tgtgggtaga accttcagcg ggttttagtt
    14641 tatctagttt catgacctta caatcgttag ttgttatacc tattgctctt caattcgcac
    14701 aaggtgacgc accttagcaa tcatacccat aacctgtggg gtagcctcta cctcaacggt
    14761 atgatgcata cggcggattc ccagcgaacg aagtgttaag cgttgccctt cggggcggcc
    14821 aataacgctg cgtatctgtg ttactttaat ctttgccata gtcatcagtt tttatccgtt
    14881 gaatactttt ttaaggtcaa ccccacgctg ctgggccacg gtgtgagcat cgcgcagttc
    14941 aagcaatgcc ataacggtag ccttaaccag gttgtgaggg tttgatgaac cttttgattt
    15001 tgcaagtacg tcggttacac ccacgctctc aagcacggcg cgcatagcac caccagcctt
    15061 aacaccggta ccgtgcgaag caggtttaat cagcaccttg gcaccaccaa acttggcttc
    15121 ctgttcgtgg gggatagtac cctttagtat gggtacctta accagattct ttttagcatc
    15181 ctcaataccc ttggcaatgg cagcggttac ttcgctagct ttacctaagc cataccctac
    15241 aacgccattc tcattaccta ctacaacaat ggctgagaag ctgaaagttc taccaccctt
    15301 ggttaccttg gttacgcgct gaatgctaac cagcctgtcc ttgagctcaa ggtcgctggc
    15361 ttttactctt cgtatgttgg tattctgtga catcgtgctt agaatttaag tccaccttct
    15421 cttgcggcat cagctaaagc cttaacgcgg ccatggtata ggttaccacc acggtcaaac
    15481 accacactct caataccctt agccatggcg tttttagcgg caagctgtcc aacaagtcgg
    15541 gcttgttcgg ttttagtaat attttttttc tctgcaatct cttttgacat cgaagaggcc
    15601 gatgctagtg ttacaccatt gatatcgtca atgaactgaa cgtatatgtt ggtgttgctc
    15661 ctgaaaaccg acatacgagg tttttgagct gttcccgaaa ctcttttgcg gattcgtctg
    15721 cgaatgcgca gctttctttc caatttattt aaagccatat ctctctttca gttttacggg
    15781 ttatacacta gccgacttac cagctttctt acgtacaact tcacccacaa agcgaatacc
    15841 tttaccctta tatggctcag gcttacgaag cgagcggatc ttagcggcaa cctgacctaa
    15901 gagctgctta tcgatgctct tcagaatgat cttggggtta ccctttttct cggtaatggt
    15961 ttcaacctta atttccttgg gcatctcaaa gtagatgtcg tgggagaaac caaggctcat
    16021 ctcaaggatt tgacctttag cttcggcctt gtaaccaaca cccacaagtt cctgctgtac
    16081 ctcaaagccc ttggatacac caattaccat gttgttgata agggcacggt atagcccatg
    16141 tagcgaacgg tgacgcttat gatcagttgg tctggagaga acaacttcat ttccctctac
    16201 ctttacggtg atatccggat caactttttg ctttagttct ccgagagggc ctttcacgct
    16261 taccacgtta tcggcgctaa tgttaacgct tactccttgt ggtaagttta caggtttttt
    16321 tcctattctc gacattagtt taaaaattat actggattag taaacatagc aaaccacttc
    16381 accaccaata ttcttggcgc gagcctcctt gtcggtcatc aacccttgcg atgtgctgat
    16441 aatggcaata cccaggccat ttaacacgcg gggaagatct tttgcgttgc tgtagcgacg
    16501 caagccggga gtactaacgc gttccaggtg cttgatggca catgccttag tctcaggatg
    16561 atacttaagt gcgattttga taatgccctg cttgccatca tcctcaaatt tatagtttag
    16621 tatgtacccc ttatcgaaaa gaatcttggt gatatccttc ttcatattcg aagcgggtac
    16681 ctcaacaacc cggtgacgag ccatcacggc gttacgaact cgggttaggt aatctgcaat
    16741 tgggtcggta atcattgctt cgttttatta ttaaattcta ccaactagct ttcttaacac
    16801 caggaattaa cccttttgag gccatttcgc ggaaggtgat acggctgatg ccaaactggc
    16861 gcatataccc tctgggcctt ccggttaaca tgcaacgatt acgcaaacgg atggggttag
    16921 agttacgagg taacttctga agcccaacgt aatcgccttc ctctttgagc ctggctctct
    16981 tttcggcgta ttttgctacg agtttcgcac ggcgaacctc acgcgctttc attgactctt
    17041 ttgccatagt ctattccttt ttagcatttt taaaaggtaa cccaaactca cgaagaagtg
    17101 cataagcctc ctcgtcgttc tccgatgtgg ttacgaaagt gatctcgaca cccagaatct
    17161 tgctcacctt atcgatgtca atttcaggga aaataatctg ttcctgaatg ccaagggtgt
    17221 aattaccacg gccgtcgaac ttgtcgttaa ttcccttaaa gtcgcggata cgcggcaagg
    17281 aaacgttgat taaacgctca aggaactcat acattctttc gcggcggaga gtaaccttaa
    17341 gcccaatggg aaccccctta cggagcttaa agtttgatat atccttacgc gagtaggttt
    17401 gaattgcctt ttgaccggca atcatggtga gttctgcctg tgcctgctca atgagtttgc
    17461 gatcggctac tgcttgtcct atcccttggt tgatcactat tttttccaac cggggaacct
    17521 gcatcacgct cttgtagttg aactgtttca tgagcgcggg gacaatctcc tccctatact
    17581 ttttctttag ggttggtacg tattccatta cttaatctcc tctcctgatt ttttagagta
    17641 acgaacaagt ttaccctggt cgttcaaccg acgaccgatg cgggttggct tgccagtggc
    17701 agggtcaacc agcattaggt ttgaaatatg aattggggct tcctttttga taattccacc
    17761 ctgagggttc ttggaattgg gtttggtatg cctcgatacc atgttagcac cctcaacaat
    17821 tgcacggttc tccttaacga gcacactgag tacgcgaccc tggtggcctt tagattcacc
    17881 ggagattacg taaaccatat cccctttctt gatgtgtaac tttgccataa ttctacagtt
    17941 attggggttt ataacacctc aggtgcaagt gaaacaatct tcatgtaatt atcacgtagt
    18001 tccctggcaa ccgggccaaa gatacgagtt ccacgaattt cgtcctggtt gttaagcaat
    18061 acgcaagcgt tatcgtcgaa acggatgtat gaaccatcct gacggcggat ctccttcttg
    18121 gtacgaacca caacggcctt tgaaacggta cctttcttaa tatcaccacc gggaatggca
    18181 ctcttaacgg taaccactat cttatcgcct atacgggcgt agcgtttacc agtacctccc
    18241 agtaccctga tgcagagcac ttccttggct ccgctattat cagctactac tagtctactt
    18301 tcctgctgta tcatgactac ttagcctttt cgatgatttc aactaacctc cagcgtttaa
    18361 gcttgctgag agggcgagtt tccatgatgc gaaccaaatc gccaacctcg catacattat
    18421 tctcgtcgtg agcgtaaaac ttggtggttt tattcacaaa ctttccgtaa atggggtgtt
    18481 ttactttacg ctttacggca acaacaattg acttttgcat cttgttgctc accaccacgc
    18541 caatactctc cttacggtta tttctcttta cttcgtttac ttgttccata gctacaacgc
    18601 attacttgtt ttgactgagc ttccgctggt tcaggacggt ttgtaacctg gcgatgttcc
    18661 tgcgggtctc tttaattttc attggattat ccaaaggaga tatgctatgg ttcaacctta
    18721 gcttaagcag gttggttttt tccagctcaa tacgctcaag aatctcctta tcggtgagct
    18781 ctcttatttc tgctactttc atggctcaat tagtttgtag gttcaacaaa gtccggacgg
    18841 acaacaaact tagtagtaat agggagcttc tgagctgcca ggcgcaatgc ttcctttgca
    18901 acttcgtagg gtacgccgtc gcattcaaac agaatgcggc ctggagtaac cggagcaaca
    18961 aacccctcag gagcaccttt acctttaccc atacgaacct cagcaggttt cttggtaatg
    19021 ggcttatcgg ggaaaattct gatccagatc tgaccttcac gcttcatgta acgggtcacg
    19081 gcctggcggg cagcctcaat ctgacggctt gtgatccagc tcgactcaag ggctttaatt
    19141 ccaaatgaac cgaatgccaa ctggtttccg cgttgggcaa tccctttcat ttttcccttt
    19201 tgctgccttc tgtattttgt tttctttggc tgtaacattt cgctaatgct ttagtgttta
    19261 tacaccttac tatttctttt tcttggcacg aggacgtgaa cctcccttag gtgctggttt
    19321 tgcctttttc tcggttacgt tggcaacgct ctttgtacca atgttgggcg aaagatcgcg
    19381 cttgccgtaa actataccgt tgcaaatcca ggtcttaata cctataacgc caaccttggt
    19441 gtgagcctcg gctagagcgt aatcgatatc ggcacggaag gtgtgtaggg gaattcgtcc
    19501 ctccttgtag gtttcagagc gtgccatttc agcgccaccc aagcggccgg aaatttgcac
    19561 cttgataccc tcagcaccca tcctcatggt tgaggcaatg gctgttttaa tagccctacg
    19621 gtaagaaatg cgtccttcaa tttggcgagc aatgttgtta cctacgataa cagcatcaat
    19681 ttcggggcgc ttcacctcaa agatattgat ttgaacatcc ttgttggtaa tcttacgtag
    19741 ctcctcgcgt agcttatcca cttcctgtcc gcccttacca ataatgatac ctgggcgagc
    19801 agtatgtatg gttacggtta tcagcttcag ggtacgctca ataactattt ttgagaggct
    19861 ggccttggca agcctggcat tcaggtagtc gcggattttg ctatcctcaa ccaatttctg
    19921 agcgtatttt ttacctccgt accagttgga atcccatcca cggatgattc ctaaacggtt
    19981 tgctattgga ttaactttct gtcccatgta gctgttagtt gtttgattcg ttattaactg
    20041 cgctatcaac cacaacggtt acgtggtttg agcgtttacg gatgcggtga gcgcgtccct
    20101 gtggtgctgt acgaatacgt tttagcattc tgccctgatc tacaaagatc tccttgatgt
    20161 aaaggttggc atcttcaatg cgtacgttct cgtttttagc cttccagtta gcaactgctg
    20221 aaaggattag cttgtgcaca tcctttgcgg catccttgcg gctgaattta agtatgttga
    20281 gcgctgtgtt tacgtcttta ccgcgaacca ggtcaaccac taagcgcatc ttacggggcg
    20341 aagacgggca gttgcgtaag cgggcaactg ctttgttttt cctctccgct ttaagcctct
    20401 cggccatgtt atgttttctt gcacccatct ctttttcctt ttatcaattc actatttgtt
    20461 cttattacca gcatggccac ggaaggtgcg ggtgggagca aactcgccca gcttatgacc
    20521 taccatgttt tcggtaatgt acacaggaat gaacttattc ccgttgtgaa ctgctatggt
    20581 gtgtccaaca aaatcgggag atatcatgga agcccttgac caagtcttga taacagactt
    20641 cttgttggat tcgttcatct gaatgacacg cttctccaac ttgtagtcaa taaagggacc
    20701 ttttttaagt gacctactca tagttacagt tttttacaaa ttactttttc ctgcgttcaa
    20761 tgatgaactt gctgctgtgt ttcttcttgg aacgagtctt ataaccctta gcaggaatac
    20821 ccttgcgcga gcgtgggtga cctcctgagg cacgtccttc accaccaccc atggggtgat
    20881 caacagggtt catggcaaca ccacgggtgc gaggacgacg acctaaccaa cggctacgtc
    20941 cggctttacc ggatttttcc aatgcatggt cagagttcga cacgctacca atggttgctt
    21001 tacactcgtt aagtaccatg cgggtttcgc cgctaggcag cttaataata gcgtatttcc
    21061 cctcacgggc tagtagctga gcatacgatc cagcgctacg tgccatgcaa gcaccacggc
    21121 cagggtacag ctcgatgttg tggataatgg tacctagggg tatttcgccc aggtaaaggg
    21181 tattacccac ctcaggagca acaccgcgac ccgacatgat ggtttggcct accttaagcc
    21241 ctgcaggggc tacaatgtag cgcttctcgc catcggggta ctcaaccagc gctatacgtg
    21301 cagtgcggtt gggatcgtac tcaatagtct ttaccacagc cggctcattc tcacggttgc
    21361 gcaggaaatc gattaatctg tatctgcgct tgtggcctcc ccctatatat cgcatggtcc
    21421 ttcggccttg gttgttgcga ccaccagtct ttttcagggg gactagtaac gacttctcag
    21481 gtttgtttgt ggtaatgtca tcgaacaaac ctataatctt gtgtctctgt cctggtgtaa
    21541 caggttttag tttccgtaca gccatcttag ttaaatgttg ctataaaaat caatcttatc
    21601 tccttcctta agcgttacga tagctttttt gtagctattg gtacggccca ccaacacacc
    21661 agtacgggtg taacgggact taactttacc ggcataacgc atggtattca ctgagtcaac
    21721 ggttaccccg tataactcct ctattgcctt ctttatctgt aacttattag ctctcttatc
    21781 cacgataaag ccgtattggt tcagcttatc ggttaagcgc tccatctttt cagttacaac
    21841 tggttttatc agtatatcca tggcgcttcc ttgttatgat accttaaaca ttttgttcag
    21901 agcatctaca gaactctcag ttaacaccaa catgttggca tttaaaatct cgtaagtgtt
    21961 tactgatgag gcaattgcag ttgcttgctt ctgtaaattt ctggacgaca aatatatatt
    22021 tttatttgtc tcgcccaaca agaagagtgt ttttttgtcg gctacgttca ggttcttgca
    22081 gatatttaca aattccttgg tttttggagt atcaaagctg aaatcttcaa taacaagtag
    22141 agcattctcc ttaaccttgt atgcaagagc gctcttacga gccagctgct taatcttctt
    22201 gtttagctta aagctatagt cgcgtggaac gggaccaaat atacgtccac caccacggaa
    22261 cacaggcgac ttgatgctac ctgcgcgagc agtatcggta cccttttggc gtttgagttt
    22321 acgggtacta ccggaaacct cgttacgctg tttggatttg tgcgtaccct gacgacggtt
    22381 agctaggatt tgctttacgt cgaggtaaat ggcatggtcg ttgggttcga taccaaaaat
    22441 cgagtcttcc aactgaatgg tcctgccggt ttcctttcct tgtatgttaa gtaccttgat
    22501 ttccattact tctcaataat tatgtaagaa ccctttgccc ctggaatcga tcctttaacg
    22561 agtaaaagat tgctctcagg gataacctta agaacacgta ggttgatgat cttcaccctg
    22621 tcgcctccgg tacggcctgc caaacgcttg ccggggagta cgcgtgaagg gaacgaagat
    22681 gcacccatag aacccgggtg acgctgacgg ttgtgctgac cgtgggttct tccgcccact
    22741 ccgttaaaac cgtggcgctt tacaacaccc tggaatcctt tccctttggt tataccaact
    22801 acgtcaaccc agttgtcatt ggcaaagagg tcaacggtta ctacatcacc aagctgtaat
    22861 ccttcccaat cgcggaattc aaccagctta cgtttgggag tagtgttggc tttcttgaaa
    22921 tggcctagca taggcctgct ggtgtgtttc tccttcttgt catcataggc aagctgaatg
    22981 gcggcatagc catcggtttc agcgtttttc acctgtgtga caacgcaagg accagcctca
    23041 attacggtgc aggggatatt cttaccatcc tcaccgaata cggaagtcat tccgatttta
    23101 cgtccaatta atccttgtgg cattgtcttt ctaatttaca attcacactc atactttaat
    23161 ctctacttcg actccactgg gaagttcgag cttcatcaga gcatcgatag tcttcgcatt
    23221 ggagctgtag atatcgagca aacgcttata ggagctgagt tcaaattgct ccctcgactt
    23281 cttgttaacg aatgttgaac ggttaaccgt aaaaatccgc ttatgtgttg gaagcggaat
    23341 tggaccgctc accacagcgc ctgtagcctt gaccgtttta acgatctttt cggccgactt
    23401 gtcaaccagg ttgtggtcgt acgatttgag tttaattcta atcttttggc tcattgtact
    23461 tatcggataa aagtaattat tcaatttctt ttagctcttt gcccttggat ttctcaatca
    23521 cctccttagc aatgttagag ggtacttcgg cgtagttcga gaactccatg gttgaagtgg
    23581 cgcgtccgga ggttagtgta cggagtacgg ttacgtaccc aaacatctcg gcaaggggaa
    23641 cctttgcctt aaccacgcag gcgttaccct tcgattccat tccggcaatc tggccacgac
    23701 gcttgttgag gtcacctatt acatcaccca tgttctcctc gggggtaacc acctcaacat
    23761 tcataattgg ctcaagtaaa accggagcgg cttttggacc tgctgcacgg aatccgtagc
    23821 gggcagcaat ttcgaacgag agagcatcgg agtcaaccgg gtggaacgaa ccatccttga
    23881 gcaccacctt catgcctggt atggcatagc cagcgagtac gccgttgctc atggctaact
    23941 caaagccctt ttgtactgag gggataaatt cacggggaat attaccaccc ttcacctcgt
    24001 caataaactg cagtccggta acaccttcgt cggctggacc aagttcaaaa atgatatcgg
    24061 cgaacttacc cttaccaccg gtttgcttct tgaacacctc acggtgcgaa acgctcctgg
    24121 taaatgcctc acggtaagca acctgaggag caccttgatt gatctcaacg ttaaactcgc
    24181 gacgtaggcg gtcaacaatg atctcaaggt gtaactcacc cataccgcta ataacggttt
    24241 gaccgctatc ggggtcggtg tttacacgga atgttgggtc ttcctctgaa agtttaccaa
    24301 gcgcaatacc gagtttgtca agatcctttt gggtcttggg ctcaatagcc aaaccgatta
    24361 caggctcagg gaaagtcatt gcctcaaggg ctataggttt ttcctccaag caaagggtat
    24421 cgcctgttct aatatcttta aaaccaactg ctgcacagat atcgccagcc tcgcagaact
    24481 caattgggtt ctgtttgttg gcatgcatct ggtaaatacg gctaatacgc tcctttttac
    24541 cggtacgggt gttgaaaacg tagctaccgc tatcaacctt tccggagtaa accctgatga
    24601 aggccagacg gcctacaaag gggtcggtag caatcttgaa tgctaaaccg cagaaaggct
    24661 gatcgatgta tggtttacga acctcttcgt taccggtgtt aggatttgtg ccaaccacat
    24721 cacccttatc gagagggctg ggtaggaagg caataaccgc atcgagcaag tgttgtattc
    24781 ccttattctt gaacgacgat ccgcaaagaa ccggaataat cgacatgtcg attgtggcct
    24841 tacggatagt ttcaataatc tcctgcttgg taatggaatc ggggtcttca aagaaacgct
    24901 caaggagctc atcgttgtac gatgcaactg cctcaataag cttggcacgc cactcgtcaa
    24961 cctcgtcctt catgttggca ggaacgggct ccagacggta gttggtagcc ttcagctttt
    25021 cgtcctcaaa gaaaacaacg gccttattct caacaagatc gataatgccg ttaaagttat
    25081 cttcggcccc gatagggagt tgaatgggaa ttggatttgc acccagtttg tctttaattt
    25141 cctgaaccac cccaaggaaa tcggcaccca cacggtccat cttgttaacg taggcaatgc
    25201 ggggaaccct gtacttatcg gcctgacgcc aaacggtttc gctttggggt tcaacaccac
    25261 ccacagcaca gaaagtggca atagcgccat cgagtacgcg gagcgatcgc tccacctcaa
    25321 cggtgaagtc cacgtgtccc ggggtgtcga taatgtttat ttgatactgc ttgttctcgt
    25381 agttccaaaa agttgtagta gcggccgagg taatagttat accacgctcc tgctcctgca
    25441 ccatccagtc catcgttgca gccccatcat gtacctcacc aatcctgtga gttttacctg
    25501 tataaaacag gatacgctcg gtagttgttg ttttaccggc gtcgatgtgg gccataatcc
    25561 caatattccg agtgaatctt aaatctctgc tcatcaatct cctaccttcc tcgtactaat
    25621 gggtcttaaa aacggaaatg tgcaaaagcc ttgttagctt cagccatctt atgcatatcc
    25681 tccttacgct tgaaagcacc accttcctgg ttgtaagcag ccataatctc tgcagcaagc
    25741 ttgtcgctca tacccttacc aacgcgtttg cgggcaaaga gtataaggtt tttcattgat
    25801 agtgagattt tacgctcagg acgaacctcg gtgggaacct ggaaagtagc accacccacc
    25861 ctgcggctct taacctcaac ctgtggggta acgttttcaa gggcaatctt ccagatttca
    25921 aggggcgatt tttcgctgtc cttcattctt ttaccaacta tatcaagtgc attgtaaaag
    25981 attccgaagg caatactctt cttacccgtt accattaagt tattcacaaa cttggtaacc
    26041 attatatcgc cgaacttggg atcgggtaag ataatccgtt tttttggttt tgcttttctc
    26101 attgttcaac caattcaaat gaggttaact tttacttttt actagcctta tccttaggcc
    26161 tcttggcgcc gtacttggaa cggccctgtt tgcgaccctc aactccggaa gcatcgagtg
    26221 ttccgcgaat gaggtgataa cgcacaccgg gcaaatcctt taccctaccc ccgcggatta
    26281 gcactatgga gtgctcctgc aagttgtgac cttcaccagg aatgtaggcg ttaacctcct
    26341 taccgttagt aagcctaacc ctggcaacct tacgcatagc cgaattcggc ttcttaggcg
    26401 tagtggtgta cacgcgtaca catacacctc gtctctgggg gcaagcatca agggctggag
    26461 ccttgctctt atccaccagt ttagaccttc cttttcttac tagttgctga attgttggca
    26521 taaatccaat ttattgttat agtttaagtt taaaaaatgg actgcaaagg tattgatttt
    26581 tttacaatta caactatgca aatcaacttt tttgtacatc agctttttta gccggtttgc
    26641 atttttgcgg gcgcaaaggt ataacttttg atttaaaaaa aacaatcctg attcttagta
    26701 acttgtaaaa aatatcggcc ctgtgcccca aaatgtttta caacataagg cttttggcaa
    26761 attttatttg ttggaaacaa aattttacta tttttgggct acccaaaaaa agggtaacca
    26821 gtcaagggaa ttggttgtaa gggatatacc ttattatata tgaatacccg cgattggaaa
    26881 aaataaaata accttatgtt aaaccaaaaa taagcgtaaa ccgcatgaaa acctaccaaa
    26941 ccgaacaaat taggaatatc gccttactgg gtaataccgg ctcgggcaaa acttcactgg
    27001 ccgagtcgat gatgtttgaa ggtaaaataa ttgaacgcaa gggaacaatt gatgggaaaa
    27061 acaccgtttc cgattacacc gaaattgaac aacagaacca aaggtcaatt ttttcaacct
    27121 tgctatacac cgagttcaat aacagcaagc taaacattat tgacactccg ggatccgacg
    27181 actttgttgg cgctgttatc tcatcgatgt acccaaccga tttgctggta atggttatta
    27241 atgcccagta cggggttgag gttggtaccg aaatcttcaa ccgtcacgcc gagcaccttc
    27301 acaagcccat gatactggcc gttaaccagc tcgaccacga taaggctaac tttgatgcca
    27361 cgctcgactc gctgaagcaa accttcggta ataaggttat cttaacccag taccccgttg
    27421 ccaccggtgc tgctttcgat agctttatcg atgtaatcac catgaaaatg tataagttta
    27481 agggtgatac cggtatccgc gaggagttcc ccattcccga cagcgaaatg gataaggcca
    27541 ctgagcttca caatgcgctc attgaggctg ctgccgagaa cgacgaatcg ctcatggaga
    27601 agttctttga gcagggttcg ctctccgagg acgagatgcg ctcaggcctt tcaaagggta
    27661 ttgccagccg cagcattatg ccggttttct gcctatcggc agctaaaaac attggcacca
    27721 agcgcctcat ggatttcatc ataaatgttg ggcctaatcc aacccactct ccagttctta
    27781 aaaacaatag cgataagccc attacctgcg acaccagcgc accaacctca ctttttatat
    27841 tcaaatcgac cgttgaacca cacattggcg aaatcagcta cttccgcgta atgtccggta
    27901 aggtaactga gggtatggac ctaaccaacg ccaacaactc aaccaaggaa cgcctttcgc
    27961 agctatttgt tgtagccggc aagaaccgta acaaggttac cgagctggtt gctggcgata
    28021 taggcgctac cgtaaagctc aagaacagta agaccaacca taccctttgc ggaccaggtc
    28081 tggatttcac cttcgatccc atggaattcc ccgatcccaa gttccgcacc gctgttaagc
    28141 ctaaaaacga ggccgaggtt gaaaagcttg gcgaactgct taaccgtgcc caccaggaag
    28201 acccaaccat tctggttgaa cactccaagg agctccgcca gattatcctt tccggtcagg
    28261 gtgagttcca cctcaatatt ctgaagtggc agcttaccaa cctacacaag ctcgagattg
    28321 aatacatagc cccacgtatc ccataccgcg aaaccattac caaggttgcc caggccgact
    28381 atcgccataa gaaacaatcg ggtggagccg gtcagtttgg cgaggttcac ctggttattg
    28441 agccctacgt tgagggtgca cccgatcccg ttaagtacaa ggttaacggt aaggaaatca
    28501 acctctcggt tagggggaag gaagttattg acctggaatg gggcggcaag ttggtgttct
    28561 ataactgcat tgtaggtgga gcaattgatg ctcgctttat gcctgccatc ctcaagggca
    28621 ttatggagaa gatggagcag ggcccgctta ccggttcata tgcccgcgat atccgcgttg
    28681 ccgtttacga cggtaagatg cacccggttg actccaacga aatctcgttt aagcttgctg
    28741 gccgcaacgc attcaagaac gcattcaagg atgccgggcc taagatcatg gagcccattt
    28801 acgatgttga ggtgctagtt cctgccgacc gtatgggcga tgtgatgagc gacctgcaga
    28861 accgccgcgc cattatcatg ggtatgggca gcgttaaggg atttgaggtg attaatgcca
    28921 aggttcccct agctgaacta aaccgctact ccactgccct atcatcgtta accagcggta
    28981 gagctaccta caccatgaag tttgcctcgt acgagcaggt tcctgccgat gttcaggaaa
    29041 agctcctgaa ggcataccag gaacaggaag aggaagaata accataccat attcagcaaa
    29101 agagggcggc caattggggt tagccctctt tttttgtacc tttgtaccaa acaaacaata
    29161 ccatgagaac agcagaaatt cacacatcga aaggggtaat gaaggttcag ttctacgagg
    29221 aggacgcccc caacaccgtt gctaactttg ttaagctagc caaggaaggc ttttacgatg
    29281 ggttagcctt tcaccgcgta atccccaact ttgtgattca gggcggatgt ccctactcca
    29341 aggatatgac cgatccccgg gtgggcaccg gcggacccgg ctataaaatt aactgcgagc
    29401 ttaccggaaa caaccagtac cacgaccgtg gtgtactttc catggcacac gcaggccgta
    29461 acacgggtgg ttcacagttc tttatttgcc acaacaggca aaacaccaag cacctcgacc
    29521 gccagcatac ctgctttggt aaggtttacg aaggccttga ggtaattgat gctatccgtg
    29581 ttggcgatag gattgaaaag attgtgattg ttgagg
//
LOCUS       DAOSAL010000026        29282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_1173_length_29282_cov_54.946454, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000026 DAOSAL010000000
VERSION     DAOSAL010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 29282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29282
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1173_length_29282_cov_54.946454"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PK049_05415"
     CDS             complement(<1..182)
                     /locus_tag="PK049_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61029.1"
                     /translation="MGSEVIIHQLLIFGVLMVVGALGSYFGIIKDEAKDFLSRFIIDI
                     TLPCLIFSTFAKIDSNP"
     gene            439..663
                     /locus_tag="PK049_05420"
     CDS             439..663
                     /locus_tag="PK049_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61030.1"
                     /translation="MKSKKDFRKPVKNDSFDSDELKRAKKLKPVKKEKNLKRSFYEEI
                     DEFEDLDDYDLKDSLDDYYDDDAYDEEEDY"
     gene            complement(728..1213)
                     /locus_tag="PK049_05425"
     CDS             complement(728..1213)
                     /locus_tag="PK049_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HOK61031.1"
                     /translation="MAETYKVDAIDQKILSYLAKNARMPFLEIARECGISGAAIHQRV
                     KKMEDAGIIAGSRMVVKPKALGFDVCAFVGVQLVSTNLYAGVIEALKMMPEVVECHFI
                     TGEYALLLKIFCRDNEHLMDVLVNTIQNIPGISRTETFISLDQAIERPVYVKDKSVIK
                     G"
     gene            complement(1355..1426)
                     /locus_tag="PK049_05430"
     tRNA            complement(1355..1426)
                     /locus_tag="PK049_05430"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1391..1393),aa:Arg,seq:cct)
     gene            complement(1497..2597)
                     /locus_tag="PK049_05435"
     CDS             complement(1497..2597)
                     /locus_tag="PK049_05435"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOK61032.1"
                     /translation="MRSLAHLLIPALFLFSCSKSNHEPTVYFTKDITPEGFMKVYKQI
                     SKNLEGKVGVKVHFGEEGNSYYVKPELFKDIVLDCKGTFIETNVLYKSPRQKTETHIA
                     LAKQHGFTYAPIDILDDKGELVIENVPGCKHFNKFYFGKNIDEYNSFLIISHFKGHGS
                     AGFGGAIKNISMGFATPRGKLAMHRNNYPVCDRDRCTNCDSCAQQCPANAISTNPIWI
                     DVDKCTGCGKCIDVCPNKALRYPKGDVDVQTVFCERLAEYAKAIYDRHKKMVFINVLV
                     DISTSCDCSRNPGKPFVPNIGILASTDIVAIEKASHDLVAKAHGCADPFLEVNRVSGY
                     RQIQYAKTLGMGKMNYRLVNLDNNQETVVISK"
     gene            complement(2765..3556)
                     /locus_tag="PK049_05440"
     CDS             complement(2765..3556)
                     /locus_tag="PK049_05440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HOK61033.1"
                     /translation="MKIKSLLLAAFAFLLLISCKTKTEKENFNQDHLIYATIWYQNSP
                     EMKALYYQGFNIAEERLKQYTKLKTAKPKAVVVDIDETMLNNVPFQAQEIIDNKEFSS
                     EFWNEWSQLARAEATPGAIEFSKLCDSLGIPIFYISNRKVNEFDATLRNLDSLGFAFA
                     KPEFIILRDNESSKKARREKVMENYEIVLLIGDNLNDFSEVFEDRSDSWGTPTVEKFK
                     NEFGSRFIVLPNPMYGDWEKNIYPSRGMLPAQQDSARRSVLKGFK"
     gene            complement(3568..4170)
                     /locus_tag="PK049_05445"
     CDS             complement(3568..4170)
                     /locus_tag="PK049_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007486937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HOK61034.1"
                     /translation="MKGSLTILSFFTLGLIVGRYTPVNDWLSSVDYSSLSLYLLMFLV
                     GISIGSDTRSLQALKSMNLKILLIPLATIIGTAIGVWFISIFVSRYSIKELMAVGAGY
                     GYYSLSSIIIRNSHSDALGVVALLSNVIREIITLLLAPLMAIYFGKVAPICSGGATSM
                     DTTLPIISRASGKEYAIPSLIHGIILTILVPFIVTLILSI"
     gene            complement(4173..4445)
                     /locus_tag="PK049_05450"
     CDS             complement(4173..4445)
                     /locus_tag="PK049_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015661 - L-lysine efflux transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903401 - L-lysine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysO family transporter"
                     /protein_id="HOK61035.1"
                     /translation="MITVVSIMAGGMLLGFLLRAKQRIVSGNEKLITYAIYLLLFMMG
                     VSIGSNEQIMNSLSTLGIVALIVSMGAIIGSILTGFLVFKLFFKND"
     gene            4542..5315
                     /locus_tag="PK049_05455"
     CDS             4542..5315
                     /locus_tag="PK049_05455"
                     /inference="COORDINATES: protein motif:HMM:NF005596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HOK61036.1"
                     /translation="MYPDYHMHTNFSDGHHTHDEMVYAAEASGIYEIGFTDHISLKPI
                     GWAMDLGRVPELIDTVNRIQHKSNSVTIRFGAEVDYIPNLVNETRGVLQSIPLDYTIG
                     SVHFIDDWNFDTDINGYQGIDIDDFYRTYFKLVGEAAQTGMFDIIGHADLAKKFAVYP
                     TFKLQKIYERCAQIFSDCGVVVELNTSGKNKPCAEFYPSVDFLEILHHYRVPITMGSD
                     AHVEQNIGQYFDEAVNLLKSLGYKEIITFNQRKRIPINI"
     gene            5392..6972
                     /gene="nadB"
                     /locus_tag="PK049_05460"
     CDS             5392..6972
                     /gene="nadB"
                     /locus_tag="PK049_05460"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HOK61037.1"
                     /translation="MSKIETDFLIIGSGIGGMSFALKVADHAKVIVVTKDRLDETNTA
                     YAQGGIAAVTYDPDTPEKHIHDTLICGDGLCNEEVVRIVVNEAPSQIKQLISWGVKFD
                     RKPNGQYDLAREGGHSEHRILHHKDNTGAEIQKVLSNKVRNHPNITVYENYFAVEIIT
                     QHHLGQEVKRGNPDITCFGAYVLNLKTQEVHTFLSRVTVLATGGTGNIYNTTTNPPVA
                     TGDGIAMVYRAKGQVENMAFVQFHPTSLYNPGERPSFLITEAMRGFGGVLKTIDGKEF
                     MQKYDPRGSLAPRDIVARAIDNEMKVRGDDYVYLDVTFKEPKEIIDHFPNIYQKCLSL
                     GIDITKDMIPVVPAAHYMCGGIKVDTNAQSTIKRLYAVGECSSTGLHGANRLASNSLI
                     EAIVYAHRGAQHSMEHYKSYEINHSIPEWDYEGTIHNEEMVLITQSYREMQQIMSSYV
                     GIVRSNLRLQRALTRLEILYRETEELYKKSILTQKLCELRNMINVGYLIIKSAQEMHE
                     SRGLHYSIDYPKQKGQEF"
     gene            7505..8206
                     /locus_tag="PK049_05465"
     CDS             7505..8206
                     /locus_tag="PK049_05465"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013352894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOK61038.1"
                     /translation="MNRIIITSLATAISVLSMAQNTLLTQRVSELNFYQLPYQYAALE
                     PAIDALTVEIHYSRHHKAYFDNFLKQASDLKLTEKSLNEIFAKVSEYPAGIRNNGGGY
                     YNHVLYWEILKPGNRKGLSPTLNKAIADEFGSVENLIAQMNDAALKRFGCGWAWLVVD
                     GNGKLKIGSTPNQDNPLMDVSDFKGIPLIGIDVWEHAYYLKYQNRRAEYVNNFWSLIN
                     WEIVSELYNEAIKKK"
     gene            complement(8251..9549)
                     /locus_tag="PK049_05470"
     CDS             complement(8251..9549)
                     /locus_tag="PK049_05470"
                     /inference="COORDINATES: protein motif:HMM:NF017396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S28 family serine protease"
                     /protein_id="HOK61039.1"
                     /translation="MKRIFSPLLLLIVICLFLLGFQRQNPVAEQVLKSIPIVDSFKVI
                     DNDPLFQTTYEVWFSVPLDHNNPNSEKFPLRAYYSHRSFTRPMVVVIDGYTMYTSRPN
                     ELTRILEANQLTIEHRFFSKSRPKDSIPWSYLNIRQAAADAHAVISAFKPHYQGKWIS
                     TGISKSGQATIFHRRFYPNDVDVSVPYVAPLNFSSEDKRVYEFLSKVGSAECRKKIRE
                     FQIALFRNKNTLLPMFEGLAQKNNWQFTMGIGRAYDLSVLEYEFAFWQWGYSCSDIPA
                     GNSQPSVLFEHWSQVNPFTFFEEKEIEKVRPFFYQAMTQIGMYGYNIEPFRNYLPDKK
                     NITFEFTLPKGHTVSFDSSAMLDVYRWVRDSGNYMLYIYGQNDAWSSTAVDPGTKTNA
                     VKMVCPSGSHSTRIKDFPKPLRDSIYITLERWLEMPVGRK"
     gene            complement(9584..11941)
                     /locus_tag="PK049_05475"
     CDS             complement(9584..11941)
                     /locus_tag="PK049_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HOK61040.1"
                     /translation="MMTERIQQLRKQSLNAVNTLSAERALLVTEYYKKADSNLPIPIL
                     RARAFEHIMLNKKICINPGELIVGERGPAPKAAPTYPEVCLHSLDDLEIINSRPKVSF
                     RVDEETREVYRNIIIPYWTGKTLREKIFAWLSNEWHQAYSAGVFTEFQEQRAPGHTVS
                     GRKVFQKGMLDLINDIDSSLKCIKQEANTREKVNELEAMRIVANAIIGFANRYADNLH
                     LLAEKETDENRKAELQKMEVICRRVPTHAPQNFHEALQHYWFIHVGVITELNPWDSFN
                     PGRLDQHLYPFYRRDIENGTITQEQAIELLQAFWVKFNNHPSPPKMGVTAQESNTYTD
                     FALINLGGVKEDGSDAVNELTYIILDVIEEMRLLQPSSMVQVSKKNPDRFVKRAVKIT
                     KTGFGQPSFFNTDAIVQQLLSQGKSLVDARNGGASGCVETGAFGTEAYFLTGYFNLNK
                     VLEITLNNGVDPLTGKKIGLETGNPENFKSFEEFYEAFERQLNYFIDIKIEGNLVVEK
                     LWAMNMPVPFLSLLIDDCIANATDYNAGGARYNTSYIQGVGLGSITDNLTAIRKHVYE
                     DGSIGMKEMLFALSSNFDGYDEFRYKLIYETPKWGNNDEYADRHAVMVFNSFYKAVNG
                     RPTYRGGVYRINMLPTTSHVYFGSKIGAMPDGRKAFEPLSEGISPVQGADRKGPTGVI
                     LSCSKIDHIKTGGTLLNQKFSPLFFSTDESLDKLVMLIRSYFKLDGHHIQFNVVSANT
                     LRDAQKHPEKYRDLIVRVAGYSDYFNDLGEALQNEIIRRTEHESL"
     gene            complement(11962..12759)
                     /locus_tag="PK049_05480"
     CDS             complement(11962..12759)
                     /locus_tag="PK049_05480"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HOK61041.1"
                     /translation="MEKGLIFDIRHFSVHDGPGIRTTVFLKGCPLRCVWCHNPESQKY
                     EPEKVQRTTKLDGKEYNSSETIGSYMTAEEVFDQVKTHIPIFDESGGGVTFSGGEPLM
                     QPKFLVNILKLCRANDIHTAVDTCGFALHEVFNLILPYTNLFLFDLKLISPQKHESYT
                     GQTNHVIFKNLFLLELNNAPTIIRIPLIPGITDDQNNLEAIKSVLKPLRCVKRVDLLP
                     YHHMAKDKYRRMNLPYKLEDLKSYTANQLGEIKSFFNDIDIPVTIGG"
     gene            13403..13582
                     /locus_tag="PK049_05485"
     CDS             13403..13582
                     /locus_tag="PK049_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61042.1"
                     /translation="MGTPRVELPRDTICHALSVAHVVSLTKPTATFHPRPIAGSGFYA
                     SAYVILCWLRNLINI"
     gene            14201..15298
                     /locus_tag="PK049_05490"
     CDS             14201..15298
                     /locus_tag="PK049_05490"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOK61043.1"
                     /translation="MRNKLLKEKRMRGYFIEAAKEILKGEGIDSMSVRNIADQAGYSY
                     ATLYNYFKDVTDVINECINDFAEECQEYVDEKTRNLPDGPEKLKAIIKSYVGYFLEYP
                     SIFDVFFLEKINKIEKKRDTSQLIVTLLERLCKPQWNYLINNEYISSSSAEKAITILR
                     YQIPGMLLFYLNRSNPDSPKEFYSLFDTQLDKLIRFEIPTRTTQTFEEVVLKFIFDGT
                     YLGENYYFFIHYTREKQVVDSILKTGFKYIESFHNSAEQIIDDKLDFLYKHNIYKPYG
                     NFIVVIGISRNIFDKYAQLIRSKGINTYIENILCDTAPEFDDEAEEYRYTLPTQYIKG
                     YVNYVTGETVKNPSFNPDYDSTNFLNNLNSL"
     gene            complement(15299..16033)
                     /locus_tag="PK049_05495"
     CDS             complement(15299..16033)
                     /locus_tag="PK049_05495"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOK61044.1"
                     /translation="MKKNAWLTILFAFILNLNETMAQQKISFPAKDGIEVTADLYFLA
                     SDKPYVILLHQARYSRGEYIEIAPKIVNLGYNCIAVDLRSGNEVNGVVNETAKRAKEK
                     GLPQNYTDAIPDIEAAIAYVKRQTNLPFVLWGSSYSASLALIQTAKDLRTKAVVAFSP
                     GEYFDTPEFVSSKIKDISVPVIVLSSKAECSSVKGLIEAIKRQNLVTQFCPTDKGKHG
                     SSALWKSNPSSNDYWLAITMFFGRLK"
     gene            complement(16037..16294)
                     /locus_tag="PK049_05500"
     CDS             complement(16037..16294)
                     /locus_tag="PK049_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HOK61045.1"
                     /translation="MICELYSVEKFFQVEDVYYVQVPGTKGSFGILKKHAPIISSLEP
                     GVVKIVRNDGAELFFAIPAGGFVGVENDVISIVSEEINQTF"
     gene            complement(16298..17809)
                     /gene="atpD"
                     /locus_tag="PK049_05505"
     CDS             complement(16298..17809)
                     /gene="atpD"
                     /locus_tag="PK049_05505"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008998434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOK61046.1"
                     /translation="MSDNFGEVVQIIGPVVDVAFPSKEKLPSIYEALELTRPDNTILV
                     LECQQHIGESTVRTIAMDSTDGLSRGMKVRATGKSITMPVGNKIKGRLLNVIGETIDG
                     LPQVDRQSNYQVHSKPPKFDQLSTQKEILYTGIKVIDLLEPYSKGGKIGLFGGAGVGK
                     TVIIMELINNIAKGYSGMSVFAGVGERTREGNDLLREMIESDVIKYGKEFKESMLKGG
                     WDLSLVDTEELEKSQATLIFGQMNEPPGARATVALSGLTVAESFRDGGEDGQGRDILF
                     FIDNIFRFTQAGSEVSALLGRMPSAVGYQPTLATEMGLMQERITSTKKGSITSVQAIY
                     VPADDLTDPAPATTFSHLDATTVLDRKIAELGIYPAVNPLDSTSRILTPEVVGDEHYN
                     TAQRVKNLLQRYKELQDIIAILGMDELSEDDKLVVHRARRVQRFLSQPFHVAEAFTGI
                     KGVMVDIKDTIKGFNMIMDGEVDQYPEAAFNLVGTIEEAIEKGERMLAEAKKQ"
     gene            complement(17880..20927)
                     /locus_tag="PK049_05510"
     CDS             complement(17880..20927)
                     /locus_tag="PK049_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOK61047.1"
                     /translation="MSNFRRANNIVGWIVFAISAIVYLLTIEPTASFWDCGEFIATAF
                     KLEVGHPPGAPLFMMIARLFALFAPDVTKVAALINSMSALASAFTILFLFWTITHLAR
                     KLIVGNSYTTSTGQLIAILGSGAVGALAYTFSDTFWFSAVEAEVYAMSSLFTAFVFWA
                     ILKWEEQADEAHSTRWIIFIAYVMGLSIGVHLLNLLTIPAIAFIYYFRKFKTTRNGIL
                     TTLAVSAIILGSILYIIIPGTFKFGSWFDLLFVNSFGLPYNSGLFFYFVLLFGALAYG
                     IYYSHKKGRVILNTVLVGVTVILIGYSSYMMILIRAYANTPINENNPSNAFSLISYLN
                     REQYGDRPLFYGQYYNAPVTDSKDLETWIPEDGKYVRGYLKTKYEFDDRFKTLFPRMY
                     SNQPDHVAEYKKWANIQGKPVQVNTRDGGTETRYVPTFGENLLFFFRYQIGHMYIRYF
                     MWNFVGRQDDVQSHGGVENGNWISGIKPIDAIFLGNQDNLTDDMKSHPSRNTYYFLPL
                     ILGLLGIYFQLQVKKDKKNFWVVFMLFFLTGIAIVMYLNQYPLQPRERDYAYAGSFYA
                     FTIWIGLGVLAVYEWFRKKLSETPSAIAATTLCLVVPVIMGTENWDDHDRSGRYIARD
                     FAYNYLNSCEPNAILFTNGDNDTFPLWYAQEVEGIRTDVRIVNLSLLGTDWYIDQMKM
                     KCYQSAPVPISMKHDQYVMGTRDVVYIINRLNEPIELKQLMDFVLSDDPAKKYRIPGE
                     KPLDYLPTNKIKLTVDKSKVLSSGTVKEKDASLIVDTMEWTLKGQYITKSALAILDII
                     ANNNWERPIYFVSPFGDGDIGIADYLQHEGFAYRLVPIKSNSSDYLNISRIDSDILYD
                     RLMNRFRWGRINEPDVFIDHNVQRTAMVLKLRNSFSRLAKKLIEEGKKDSALKVLDRA
                     VELLPHPKFPYDFFMFGIIEGYYKLNEVQKANKLTLDYAKVSEQKLRYYVSLDDRMKQ
                     YYQDDIGLTAQTLQELASIAETYGQNEVAVDINKMIQAALTLK"
     gene            21184..21522
                     /locus_tag="PK049_05515"
     CDS             21184..21522
                     /locus_tag="PK049_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013545811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HOK61048.1"
                     /translation="MDWKDKLNFAYSTNPDYKPNDEDDNTTREVVPPSKQRLIVGIER
                     KNRGGKCVTIVKGFVGSDDEIEKLGKTLKTRCGVGGTVKDGEIIIQGEIKQKVASILQ
                     ELGYRVTISG"
     gene            21527..22786
                     /locus_tag="PK049_05520"
     CDS             21527..22786
                     /locus_tag="PK049_05520"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOK61049.1"
                     /translation="MRTKIIIIGFLLFAYRLSLADTLTVMQYNLMYYDKEYSDCNATN
                     NNVDAKDGYLRTILNHVKPDILSVNEVNDAIASVERIKTNTLNYGGETRYKRANFSGS
                     FLVNMIYYNSEKLELKSQDAIATSPRETNVYKFYLKTSGLVNGDTIFLYHFVTHLKAG
                     SYESDAQQRAVAANSIMSYISTKNIKGNVILSGDMNLYSASEGAFVAFTTPVGSNNFR
                     FYDPLNAVGSWHSNYSYRYVHTQSTRTVASEGCFSAGGLDDRFDFILSSNDILAGTSG
                     IKFYSYKTLGQDGNRYNGSITSPTNTSIPTDVANALYGMSDHLPVVMKVTYSSAPTQV
                     NTEKNQVSILYNNPVYNTLNVQILENTSIKAINVYNLLGNCILNLIPESNSQKVDVDL
                     SGAPTGVLLVEVRLNDGRKKTIKVLKQ"
     gene            22802..25642
                     /locus_tag="PK049_05525"
     CDS             22802..25642
                     /locus_tag="PK049_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61050.1"
                     /translation="MFNKKIYFFTLILIILINNSKAQYYQTGVEPFKTDWKKREYKNI
                     RLIYPAEADSLARHYLSALAKSDSLNDVDYNLGTRKIDVVLHPNTVLSNGFVAWAPRR
                     MELVTQPDADGDALLWHKALSIHEMRHVKQMYALNSGSTKVLSWLFGQQAVGFASSFI
                     HPWIFEGDAVWAETNYTFSGRGRSATFFNHYYAHLVSKQKPYTYDKWLLGSYKDFIPN
                     HYQFGYQLVSFINQKWGSTTIPKTYRYVGRYPFTLFPTYLSLKKFTGQSRKSIYQKAF
                     SYNDSLWLVDYTEHYGNELPKAVNEDFKNLIHPYPLTDTTTIVYVTSLSDTPYFAVLD
                     KRGGIKKIVSTGILIGQPSYNDSLIVWAEYQPHARWEWMSSSAIKVYNIKTKRLNTIP
                     GGRYHSPIIDEDRIICIEYSSNGQYKLVGINFLGEKSTLLNFEGGYEVQQICLDTKGK
                     NIYGIAVSETGKKIFKVDSALTFDIVYNAAYRDIRTLACDGSNLFFTFTDGYTESIYK
                     YDIQNNIVYKIINLPFNSSYPTYKNHQLYYAHYTANGYRIAFLDDITSNRKIVDNISP
                     YNEIVKVNPTTVHDNYGIIQTEPTEYKGLNTLVNIHSWFPFYTKPLNDPANIEDESNI
                     YPGITLLSQNLTGSTLFNAGYGYGKTHLLFAEVTYNGLWPVFRLNIEKTDATASLYRV
                     TQFYPENRDYYKKAEFTSILPFTLGESIYSTSLQLYNKTIYSNSYLYRESIDAYRSGL
                     ITNEFGFTFYSLRRMAHRDLQPKSGLIINTGLLTTPWDYGNLPNLWYSQAKIYLPGLH
                     RNHGFGITLLAQKQNIKYIYLNNKVNFPAFYTSRPSTRFTGMYFNYIMPIAYPDLPIS
                     SLVYVKRISLNLFADFAKNSYKTYQQYELVTLTDNLQSIGFDAIVDFHLFRTWYPFRI
                     RFTQAFNGSDFNPYSNIVLTINLYSTFARPKL"
     gene            25862..26407
                     /locus_tag="PK049_05530"
     CDS             25862..26407
                     /locus_tag="PK049_05530"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOK61051.1"
                     /translation="MSKNELKNQIRKAIKSHRLLLDPFLARERASYVFAEIESMEQFQ
                     KATTVLAFWSLPDEINTHDFVIKWAGSKRMVLPVVVGDELELRLFNGVENMEKSGGFG
                     IMEPKTGSLVNPDEIDFAIIPGVAFDRNGNRLGRGKGYYDRTLPLLRNAVKVGIAYEF
                     QIIDSVPVSEHDIPVDLVVSN"
     gene            26420..28666
                     /locus_tag="PK049_05535"
     CDS             26420..28666
                     /locus_tag="PK049_05535"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOK61052.1"
                     /translation="MTGGFRRALTLGLLILTLSVKIFAQIPDGYYATAEGKTGAELKT
                     ALYNIIKNHTVISYDGLWTAFQYTDKKSNGKVWDMYSDKPGQTPPYEFTFVTDQCGNY
                     SSEGDCYNREHSFPKSWFNDASPMYSDLFHLYPTDGYVNNRRSNFIFGEVTSVTWTSL
                     NGSKLGTSSASGATLTVFEPIDEYKGDFARTYFYMATRYENLIATWASYNTEAKAILD
                     GTAYPAYKQWYITLLLKWNQQDPVSQKEIDRNNVVYSSYQHNRNPFIDHPEYAQLIWG
                     STTPITFTSTPITSATVGVTYTYNVTATGGNGTPLTISATQKPTWLTLTATGNGTATL
                     SGTPGQENVGGNQITLKATDGISETQQTFTITVSSPVTPIQFTSTPITTAVENQLYTY
                     NATATGGNGAPLTITATQKPAWLTLTSTGNGTATLSGTPDHSNIGNSQVVLAATDGTN
                     QGTQSFTINVSAAGNSKPLITNVNITPASPVTRQAITISANITDSDGSVAKVLLGWGV
                     TVGNLSNTYEMQFENGAYSAPIASQTSPGTIYFQLAAIDNLQDTAYYNGSFTVNLNQV
                     PTISSVSHTPTNPTSADAVTVTANVSDPEGRIGNIFLLWGITESSLINQLQMSGTNGV
                     YTSVIPANPSQTSVYYRVKAYDAEGQQTLSSISSYIVNPATEIRTETSKQFLVFPNPF
                     NAFIQVEYPSAESYSVTFTNLIGQVVYSKTRVTGNLRIDCSDLQSGIYVLTIKSDTGI
                     VTQKMIKR"
BASE COUNT         8781 a         6037 c         6018 g         8446 t
ORIGIN      
        1 gggttactat caattttagc aaaagttgaa aaaatcaggc agggaagggt aatgtcgata
       61 atgaagcgcg acaggaagtc tttggcttcg tccttaatga ttccgaagta ggagcccaaa
      121 gcgcccacta ccattaaaac gccaaaaatt agtaactggt ggataattac ctcggacccc
      181 atatagcggt ttaaaacttc acaaatgtac gtaaatgtgt ttatgtaaaa aaaatagttg
      241 tttgatgaaa aagtattgac ttttatatag ctgatgtgta acttggtaaa ataatatgct
      301 ttaaacgtaa aaattttcac catacagtag gccaatacct tgcaacgaaa aaaaaaatac
      361 ttaaaattgc ggttgatata tttaatgttt aaccctaaat agttaaaaaa caaaggaaac
      421 atcttaattt gtcttgtgat gaagtcaaag aaagattttc ggaaacccgt aaagaacgat
      481 tctttcgatt ccgacgagtt aaagagagcc aaaaagttga aacctgttaa aaaggagaag
      541 aatttgaagc gctcttttta tgaagagatt gatgagtttg aagatctgga cgattacgat
      601 ttgaaagact ctttggatga ttactatgat gacgatgcct atgacgaaga agaagattac
      661 taaaaaataa aataaaatta aggctgaacc tagggttcag cctttttttg ttgtatggat
      721 tattgtttta acctttaatc acacttttat ccttaacgta aaccgggcgc tcaatggctt
      781 ggtcaaggga aataaaagtt tctgttcttg aaataccggg gatattctga atggtgttaa
      841 ccaacacgtc catcaggtgc tcgttatcgc ggcagaaaat ttttagtaaa agggcatatt
      901 cccctgtgat aaaatggcac tccaccacct cgggcatcat tttaagagcc tcaattactc
      961 ctgcgtaaag gttagttgat acaagctgta ccccaacaaa agcgcaaaca tcaaatccta
     1021 gcgctttggg tttaacaacc attcgcgaac cggcaataat accggcatct tccattttct
     1081 taacccgttg gtgaatagcg gctcccgata ttccacattc gcgagcaatt tccaaaaaag
     1141 gcattcgtgc attcttagcc aagtacgata gtattttttg gtcaattgca tcaaccttgt
     1201 aagtttcagc catagaagtt acaatatgta attaaaattg gtataaaaat aatgcaatga
     1261 taaaaaaaat acaagaaatg ttacaaactt ttacatattt ttcctcatgt ttggtggagt
     1321 gaaaataaaa aaaggggaag aactcccctt gtagtggcct taccaggact cgaacctgga
     1381 tctaaagttt aggaaacttc cgttctatcc cttgaactat aaggccaaat tgctcggcaa
     1441 agatattact taatttattt ttttgcaaca ggtttgcgat tgttggttta gaaactttac
     1501 tttgaaatta ctacagtttc ttggttgttg tccaggttaa ccaggcgata gttcattttg
     1561 cccatgccta acgttttagc gtactgaatt tgcctgtaac cgcttacacg gttaacttcc
     1621 agaaatggat cagcgcatcc gtgggcttta gccactaggt cgtggcttgc tttttcaatg
     1681 gccacaatgt cagttgacgc caaaatacca atgtttggga caaatggctt tccggggttt
     1741 cgggagcaat cgcacgatgt tgatatatcg actaagacat ttatgaaaac catcttttta
     1801 tgacggtcgt aaattgcctt agcgtattca gcaagtcgtt cacagaatac agtttgaaca
     1861 tctacgtcgc ctttagggta acgtaaagct ttatttgggc aaacatcaat gcattttcca
     1921 catccggtgc atttatctac atcaatccaa ataggattag ttgaaatagc atttgccggg
     1981 cattgctgtg cgcagctatc gcagttggta cagcgatccc tatcgcatac agggtaattg
     2041 ttcctgtgca tggccagttt ccctcgaggg gttgcaaacc ccatcgatat atttttaatg
     2101 gctcccccaa aaccagccga tccatgcccc ttaaagtgcg agattatgag gaaagaatta
     2161 tactcatcaa tatttttccc gaagtaaaac ttattgaagt gtttacatcc gggtacattt
     2221 tcaattacaa gctccccttt atcatctaaa atatcgatag gggcataggt aaatccatgt
     2281 tgtttggcca gcgcaatatg ggtttcggtt ttttgccgcg gacttttata taatacattg
     2341 gtttcaatga atgttccctt gcagtcgaga acaatatcct taaatagttc cggtttaacg
     2401 taataggaat tgccttcttc gccaaaatga acctttacgc ctactttccc ttcaaggttt
     2461 ttggaaatct gtttgtaaac cttcatgaac ccctccggtg taatatcctt ggtgaagtaa
     2521 acggttggtt catgatttga ctttgaacat gaaaacagga aaagggcagg tattaacagg
     2581 tgggctaagc ttctcatagt attttatatt tgatgtaaaa catacttaat aaagcaatct
     2641 cattcaaaat tagcaaatta atgcaagtgg ttaataaaca aaaggatgat taatcataat
     2701 tgatattttt tctaaataaa aaagggcgaa aaatcgccct tttttggtga gagaattggt
     2761 gctcttactt aaagcccttt aacacagagc ggcgtgcact atcctgctgg gcaggcagca
     2821 tgccacgact cggatatata tttttttccc agtcgccgta catggggtta ggcaaaacta
     2881 taaatcggct tccgaattca tttttgaatt tctccacggt gggtgtaccc cagctatcgc
     2941 ttcggtcttc aaaaacctca gaaaaatcgt taaggttatc gccaataagc aaaactattt
     3001 catagttttc cattaccttt tcgcgacggg cttttttgct actctcattg tccctgagaa
     3061 ttatgaactc tggttttgca aaggcaaaac ccagcgaatc gaggttgcga agggtagcgt
     3121 caaactcgtt tacctttcgg ttgctgatgt agaaaatcgg aatgcccaac gaatcgcaaa
     3181 gcttgctgaa ctctatggca cctggggtgg cttcggccct ggcaagttgg ctccactcgt
     3241 tccaaaattc actcgagaac tctttgttat caattatttc ctgcgcttgg aatggtacat
     3301 tgtttaacat ggtctcatca atatcaacca ccactgcttt gggttttgca gtttttagtt
     3361 tcgtgtactg tttcaatctt tcctctgcaa tattgaagcc ttggtagtac agggctttca
     3421 tttcggggga gttttggtac caaatggtgg cgtaaatcag gtgatcctga ttaaaattct
     3481 ccttttcggt ttttgtttta caggagatta acagcaaaaa cgcaaatgct gccagcagca
     3541 aagatttgat tttcatggct ttgttattta gattgataga attaacgtta caatgaaagg
     3601 caccagtatg gtaagtatta ttccatgaat aagcgatgga atggcatact ctttccccga
     3661 tgctcttgaa attattggca atgtggtatc catgcttgtg gctcctcccg agcagattgg
     3721 tgcaacttta ccaaaataga ttgccataag cggtgccagc agcagggtaa taatttcgcg
     3781 aattacattc gatagcaaag caacaacgcc aagtgcatcg ctgtggctat tcctgataat
     3841 tatacttgat aggctgtaat agccataccc ggctccaaca gccataagtt ccttaataga
     3901 gtatcgtgaa acaaaaattg aaatgaacca aaccccaata gcagtaccaa taattgttgc
     3961 taagggtatc agcaggattt ttaaattcat gcttttgaga gcttgtaacg atcgggtatc
     4021 ggaacctata ctaattccta ccaaaaacat aagcaggtaa agcgataggg agctgtagtc
     4081 aacggaacta agccaatcgt taactggagt atatcgacca acaattaacc ccagcgtgaa
     4141 aaatgaaagt atggttaaac tccctttcaa ctttaatcgt ttttaaaaaa caatttaaac
     4201 accaaaaaac ctgttagaat gctcccgatt atagcaccca ttgagactat tagggccaca
     4261 ataccaagcg tactcaacga gttcataatt tgttcgtttg aacctatgct aacgcccatc
     4321 atgaaaagaa gcaggtatat ggcataagtg ataagcttct cattaccgga aacaatacgt
     4381 tgctttgctc ttaaaagaaa accaagcaac atacccccag ccattatgct tacaaccgta
     4441 atcaaatcat caaaaatttt ggcaataata gcaataaatg tgataaattt gatatgaaca
     4501 cgaaacgttt ggatttgttt ataaactaaa atcgatataa aatgtacccc gactaccaca
     4561 tgcataccaa tttttctgac ggacatcata cacacgatga aatggtttat gcggcagagg
     4621 catcagggat ttacgaaata ggtttcacgg atcatatatc gcttaaacct attggctggg
     4681 caatggattt gggaagagtt cctgaactga ttgacacagt aaatcgtatt cagcacaaat
     4741 ccaattcagt aaccattcgg tttggagccg aggtggatta tattcccaat ttggttaatg
     4801 aaacccgagg tgttttacaa tctattccct tggattatac cattggttcg gttcacttta
     4861 ttgatgattg gaattttgat accgatatta acggttacca aggcattgat attgatgatt
     4921 tttaccgcac atatttcaaa cttgttggcg aggcagcaca aaccggaatg ttcgatatta
     4981 ttggtcatgc cgatttagca aaaaaatttg cagtttaccc tacgtttaag ctgcagaaaa
     5041 tttacgaaag gtgtgcacaa atttttagcg attgcggtgt ggtggttgag cttaatacca
     5101 gcggaaaaaa taaaccctgt gccgaatttt acccatcggt cgactttctc gaaatccttc
     5161 atcactaccg ggtacccatt accatgggta gcgatgctca tgttgagcaa aatattgggc
     5221 agtactttga tgaagccgtt aacctcctta aatcgttagg atacaaggaa ataattacct
     5281 ttaaccaacg aaaacggata ccaatcaata tttagctttg ataacagcct actaattcat
     5341 agttttgctc aaacataaca cggcaattta ttgctgtata cctttaaccc gatgagcaag
     5401 attgaaaccg atttcctaat aattgggtca ggcataggcg ggatgagttt tgcccttaaa
     5461 gtagccgacc acgcaaaggt tatagttgtt actaaggata gattagacga aaccaataca
     5521 gcatacgcac aggggggcat agccgctgta acatacgatc ccgatacgcc cgaaaagcat
     5581 atacacgata ccctcatttg tggtgatggg ctttgcaacg aggaagttgt ccgcatagtg
     5641 gttaacgaag ccccttccca gattaagcag cttattagct ggggcgttaa gtttgaccga
     5701 aagcccaacg gtcagtacga tttggctcgt gagggcgggc actcggagca ccgcatacta
     5761 caccataagg ataataccgg cgcggaaata caaaaggttt taagcaacaa ggtaagaaat
     5821 caccccaaca ttaccgttta cgagaattac tttgctgttg aaataatcac ccaacaccac
     5881 cttggacagg aggttaaacg cggaaatcct gatattacat gtttcggagc ttacgtacta
     5941 aaccttaaaa cacaggaggt acacactttc ctgagccgag taactgtttt ggcaactggc
     6001 ggaactggca atatttacaa cacaactaca aaccctcctg ttgccacagg cgatggcatt
     6061 gccatggttt accgggcaaa gggacaggtt gaaaatatgg cctttgtgca gtttcacccc
     6121 acatcgcttt acaatccggg cgaacgcccc tcgttcctta taaccgaagc catgcgaggg
     6181 tttggtggtg tgcttaaaac catcgacggt aaggaattca tgcagaagta cgaccctagg
     6241 gggtcgcttg caccgcgcga tattgtagcc cgagccatag ataacgagat gaaggttagg
     6301 ggcgacgact atgtttacct cgatgttacc ttcaaggaac caaaggagat aattgaccat
     6361 ttcccaaaca tctaccagaa atgcttatcg cttggcattg acattacaaa ggatatgata
     6421 ccggttgttc ctgctgcaca ttacatgtgt ggtggtatta aagttgatac taatgctcaa
     6481 tcaaccataa aaaggcttta cgcagtaggt gaatgctcat caaccggttt acatggtgcc
     6541 aataggttag cctcaaattc gcttattgag gcaattgttt atgcgcatcg tggcgcacaa
     6601 cattccatgg aacactataa gagctatgaa ataaaccact ctatacccga atgggactac
     6661 gaaggaacaa ttcataacga ggagatggta cttatcaccc agagctaccg tgagatgcag
     6721 cagattatga gtagctacgt gggaattgtt cgttcaaacc tccgcttaca gcgagcatta
     6781 actcgcctgg aaatacttta ccgcgaaacc gaggaactct acaaaaaatc aattcttacc
     6841 caaaaactat gcgaactccg gaatatgatt aacgttggtt acctcataat aaaatcagct
     6901 caggaaatgc acgagagtag agggctacac tactcaattg attatccaaa gcaaaaaggg
     6961 caggagtttt aggatgtctg ggttaattga ttttacccag agctattttt tctgcatatc
     7021 tatctgtaaa caacggccaa aataaactct cattactatt ttagagtctt ttaagcactt
     7081 gttattttat cgagaagtca atagaaaaac aaaaaacatt taaacatcct gcttctattg
     7141 acttatcgag ataaccccga cttttcaaaa tctttcattc cgagatcgcg gaatgatttg
     7201 tttttattag tcaattcgaa aaattgaatt tttcaattgg cgaatttcca ataaaatgtt
     7261 tctgtattca cattttaatt agcctggttg tatttgttaa tccatggcag tttggtttaa
     7321 ccgttcacat tttactattc ctctaacttc ctctaactcc atcatattct gtatattccg
     7381 actccttcct ctataattag actggctgaa atcgttaatt catggcactt gggtttaagt
     7441 tatagcactt attttcttga acttttcact cctgtgtgtt gtttatacaa aaaattcagg
     7501 aatcatgaat aggataatta ttacttcatt agccacagcg atatcagtac tttcaatggc
     7561 acagaatact cttttaacac aaagggtatc ggaactcaat ttttaccaat tgccttacca
     7621 atacgcagct ttagagccag caattgatgc cctaaccgtt gaaattcact acagccgtca
     7681 ccacaaggct tacttcgata attttttaaa gcaggctagt gacttaaagc tgaccgaaaa
     7741 atcgcttaat gaaatttttg caaaagtgtc tgagtaccct gctggaatta ggaataacgg
     7801 agggggatac tataaccatg tactttactg ggaaatacta aaacctggga atcgaaaggg
     7861 tttgagccca accctaaata aagcaatagc cgatgagttt ggttcagttg aaaaccttat
     7921 tgctcaaatg aacgatgctg cactaaaacg ttttggttgc ggttgggcct ggcttgtggt
     7981 cgatggcaat ggaaagctca aaattggctc tactcccaat caggataatc cgctaatgga
     8041 tgtgtccgat tttaagggca tcccccttat agggattgat gtttgggaac acgcttacta
     8101 ccttaaatac cagaataggc gtgctgaata cgtaaataac ttttggagcc ttataaactg
     8161 ggaaattgta tcggagctat acaatgaagc gattaagaaa aagtaatttt tcccccagaa
     8221 aaaatgcaac ccggtaaagg ttgcattttt ttactttcta ccaaccggca tttcaagcca
     8281 tctttcaagg gtgatataga tactatcgcg cagcggtttt gggaaatcct taatgcgggt
     8341 tgagtggcta cctgacgggc aaaccatttt aactgcattg gtttttgtgc cggggtcaac
     8401 tgcggttgaa ctccatgcgt cgttctgtcc gtaaatgtag agcatgtagt tgcccgaatc
     8461 ccttacccac ctgtaaacat cgagcatggc tgaagagtcg aagctcaccg tgtgaccctt
     8521 aggaagggta aactcaaagg tgatattttt tttatcgggg aggtagttcc tgaatggctc
     8581 tatgttatag ccatacattc caatttgggt cattgcctga tagaagaatg gccttacctt
     8641 ttctatctct ttttcctcaa aaaaggtaaa gggatttacc tgagaccaat gctcaaaaag
     8701 aaccgaaggc tgtgagttgc ctgcagggat atcggagcag ctataccccc attgccaaaa
     8761 ggcaaactca tactcaagta cgcttaagtc gtaagcacga ccaatcccca ttgtaaattg
     8821 ccaattgttt ttttgagcaa ggccctcgaa cattgggagt aaagtgttct tattcctaaa
     8881 gagtgcaatt tgaaattctc taattttttt tcggcattcg gcagagccta ccttgctaag
     8941 gaattcgtaa acacgtttat cttcacttga aaagttaagt ggagccacgt aaggaacgct
     9001 aacatcaacg tcgtttgggt aaaacctgcg gtggaaaatg gtggcttgtc cgctcttgct
     9061 tatgccagtc gaaatccatt tcccctggta gtggggttta aatgccgata ttacagcatg
     9121 cgcgtcggct gcagcctgac gtatgttaag gtaactccat ggaatggaat ccttaggacg
     9181 gcttttggaa aagaaccgat gttcaatggt tagctgatta gcttccagaa tgcgggtaag
     9241 ttcattgggg cgggaggtgt acatagtata cccgtcaatt actaccacca tgggacgcgt
     9301 aaagcttcgg tgcgaatagt atgcccttaa aggaaatttt tcgctatttg ggttattgtg
     9361 gtcaagcgga acgctaaacc atacctcgta tgttgtttga aataaggggt cgttatcaat
     9421 caccttaaaa ctatcgacaa taggtattga tttaagaact tgctcagcaa caggattttg
     9481 ccgctgaaaa cccagtagga acaagcaaat aacaatcaaa agcaagaggg gtgagaaaat
     9541 tcgcttcata acagtttgtg taaggttcat ctaaaataat actttacaat gattcatgct
     9601 ctgttcttcg tataatttcg ttttgcaagg cctcgcccaa atcgttaaag tagtcggagt
     9661 agccagcaac gcgaacaatc aggtcgcggt atttttctgg atgtttttgc gcatcgcgta
     9721 gggtattggc gctcaccaca ttgaattgaa tgtggtgtcc atcgagctta aagtagctgc
     9781 gtattagcat cacaagttta tcaaggcttt catcggtgct gaaaaacaag ggcgagaatt
     9841 tttggttaag aagcgttcct cctgttttaa tatggtcaat tttggagcac gataggatta
     9901 ctccggtagg ccctttgcgg tcagcaccct gaactgggct aatcccttcg cttagtggct
     9961 caaaagcctt ccggccatcg ggcattgctc caattttgct cccaaagtaa acatggctgg
    10021 tggtgggtaa catatttatg cgatataccc caccacggta tgttgggcgt ccgtttacag
    10081 ccttgtaaaa actattaaaa accattactg catgcctgtc ggcatactcg tcgttgtttc
    10141 cccactttgg ggtttcataa atcagtttat agcgaaattc atcgtaacca tcaaagtttg
    10201 acgataatgc gaacaacatc tctttcattc caatgctacc atcttcgtaa acgtgttttc
    10261 taattgcggt cagattatcg gtgatacttc ctagtccaac cccctgaata tagctggtgt
    10321 tatagcgtgc acctccagcg ttatagtcgg ttgcgttagc aatgcaatcg tcaataagta
    10381 gcgaaaggaa tggaacaggc atattcattg cccaaagttt ttcaactact aggttgcctt
    10441 caattttaat gtcgataaag tagttaagtt ggcgttcaaa ggcttcgtag aattcctcaa
    10501 atgatttgaa gttttccgga ttgccggttt ccagaccaat ttttttgccg gttaaggggt
    10561 caacgccatt attgagcgtg atttcaagaa ccttgttaag gttaaagtaa ccagttagga
    10621 agtatgcctc tgttccaaat gcaccagttt ccacacaacc actggcgccg ccatttcggg
    10681 catcaaccaa cgattttcct tgactcaaca gctgctgaac aattgcatcg gtgttaaaaa
    10741 acgagggttg accaaatcct gtcttggtta tttttacggc tctctttaca aacctatcag
    10801 ggtttttctt acttacctga accatgctgc taggctgcag caatcgcatt tcctcaataa
    10861 cgtcgagaat gatgtatgta agttcattta cagcgtcgga accgtcttcc ttaaccccac
    10921 caaggttaat aagcgcaaaa tcggtatatg tgttactttc ctgtgcagta acacccattt
    10981 tgggaggcga tgggtgattg ttaaacttaa cccagaaagc ttgcagaagc tcaatggcct
    11041 gctcctgggt aatggtcccg ttttcgatat cccgacggta aaaagggtaa aggtgctggt
    11101 cgagtcttcc agggttaaat gaatcccaag ggtttaactc tgtgataacg ccaacatgaa
    11161 taaaccagta atgctgaagc gcctcgtgaa agttttgtgg agcatgagtt ggcactctgc
    11221 ggcaaataac ttccattttt tgcagttcgg ccttccggtt ttcatcggtt tccttttcag
    11281 ccagtagatg taggttatcg gcatagcgat tggcaaagcc aattatggca tttgctacaa
    11341 ttcgcatggc ctccagctca ttaacctttt cccttgtatt tgcttcttgt ttaatgcatt
    11401 taagcgatga atcgatgtca ttaatcaggt cgagcattcc cttttggaat acctttctac
    11461 ccgataccgt atgtccgggt gccctttgtt cctgaaactc agtaaaaaca cctgcgctat
    11521 aggcctgatg ccattcattg cttagccaag caaaaatttt ttcacgtaag gttttacctg
    11581 tccagtaggg tataatgata ttgcgataaa cttcacgtgt ttcttcatca accctaaacg
    11641 aaaccttagg gcgggagtta ataatctcca aatcgtcgag gctatgcaag caaacctcag
    11701 ggtaggttgg tgcagccttg ggggctggtc cgcgttcacc aacaatcagc tcgcccgggt
    11761 tgatacaaat ctttttgttt aacatgatat gctcaaaggc ccttgcccta aggatgggta
    11821 taggtaaatt actatcggcc tttttgtagt attcggttac cagcaatgca cgctcagctg
    11881 aaagtgtgtt tacagcattg agactttgtt tgcggagttg ctgaattcgt tctgtcatca
    11941 taatgtaaga ctttgttgtg tttaacctcc aattgttaca ggaatatcta tatcattaaa
    12001 gaatgattta atttcgccaa gttgattggc agtatatgac ttaagatctt ctagtttgta
    12061 gggtaaattc atacggcggt atttatcctt agccatatgg tggtagggta gtaaatcgac
    12121 acgcttaaca cacctcaatg gttttaatac tgatttaatg gcctcaagat tgttttggtc
    12181 atcggttatt cctggaataa gagggattct tattattgtt ggggcgttat taagctcaag
    12241 taagaaaagg tttttgaata ttacatggtt tgtttgcccg gtgtaacttt catgcttttg
    12301 gggcgaaatc aacttcaagt caaaaaggaa aaggtttgta tagggtaaaa tcaagttaaa
    12361 gacctcatgc aatgcaaagc cacaggtgtc aactgctgtg tgaatatcat ttgcccggca
    12421 aagcttaagg atattaacta aaaactttgg ttgcattagc ggttcgccac ccgagaaagt
    12481 tacccctccc cccgattcgt cgaatattgg gatgtgagtt tttacttggt caaaaacctc
    12541 ttcggcagtc atgtaactac caatagtttc ggatgagttg tattccttac catcgagttt
    12601 ggttgttcgt tgaacctttt ccggttcata tttttgactt tcggggttgt ggcaccaaac
    12661 acagcgcagt ggacaaccct taaggaaaac agtagttcta attccaggac cgtcgtgaac
    12721 cgaaaaatgt cgaatatcaa aaattaaccc tttttccatt tgaaaaaatt aaatgattga
    12781 atgtcaacta aagggattag cgttctaatc cttttgaaaa tagatattgg aaaaagggaa
    12841 atcagcaaat ccagcaggca aaaagaccct tgccgttgtt gtggtaaagc gtatgtagcg
    12901 gtttgctgct catgtgagct acctaatcat ttccaaacaa aattaagttt ttttacaccc
    12961 aaatgcaatg agaaaagtca gtttacagat tatctaccag taatccctga acactttatc
    13021 gctgaagtat ttaaactttt tcgagaatca acataaagta ttttcttcaa aattttattg
    13081 aatgtcgttt agttaacaaa aatatacatg actgtcatcc tgcgcgcagc gaatgatttc
    13141 tattcgaccc cataagatgt tttgctaacg ctcaacatga caatagtttc acgtacaacg
    13201 cttccagccc cgtaggggcg taatatttgt aacactatga ttattccaac atgtatagcg
    13261 atgcacgcaa tgctttttcc aaagagcaag ggaaaagtgt tgcatcgtat cagaaaagcg
    13321 tggcaaagag cggtttttat gttctttctt gtcttgatac aagaaagaac caaagaaaat
    13381 caaggctgaa aagcccgacc cgatgggtac cccgcgggtg gaactgccac gcgatactat
    13441 ttgccacgca ttaagcgtgg ctcatgtggt atcgcttact aagcccaccg ccacgttcca
    13501 cccccgaccc attgccgggt caggctttta tgcctctgct tatgtcattc tgtgttggct
    13561 gaggaatctc attaatatat agggatgaaa tcacaaagag atgcttcgac ttggctcagc
    13621 atgacaaagg gagtgaggga gccaatggta ttgtcattcc gagctagtcg aggaatcttc
    13681 ttcaagtata ccgcgaccag ccctgtaggg gcgaaacatt tttaacccca tgtctattcc
    13741 cacatgcata gcgatacacg ccatacatat gccgcaagag tgaaacgttg catcgcaact
    13801 gaagagcatg gcaaagagcg gtttacaaaa aagcaacaag gattaaaggt aaagagatgc
    13861 ttcgactagc tcagcatgac aaggaagcag aaataaagat agggtttttg taatcccgag
    13921 caaaggcgag ggatctcttc aaaggattga agagaaaaga gcaatgagat gctacggcga
    13981 tgtctatccc taaaaatcga aactcactac gacttgtgat gtttcgcttg cgcgcaacat
    14041 gacaaatacc cttaactaaa cgacattgaa attttattaa aaagaaagtt gcaggaactt
    14101 tacctttagg gaaaaatagt atttgtttga tctttttttg gttaacattg ttaaataaat
    14161 taacagcgtt aattatattt gtacaaaatt cggtaaatcg atgcgtaata aactccttaa
    14221 agagaagcgg atgcgtggct acttcatcga agcggctaag gaaatcctta agggggaggg
    14281 catcgatagt atgagcgttc gtaacatagc cgaccaggct ggctactcct atgcaaccct
    14341 atataactac tttaaggatg ttaccgatgt aatcaacgag tgcataaacg actttgcgga
    14401 ggagtgtcag gagtatgttg atgaaaaaac ccgaaacctg cccgatgggc ctgaaaagct
    14461 taaagccata attaaaagct atgtgggata ctttttggag taccccagca ttttcgatgt
    14521 ttttttcctt gaaaaaatca ataagattga aaagaaaagg gacacctcgc agctaattgt
    14581 tacacttctt gagcggttat gcaaaccaca atggaactac ctgattaata atgagtacat
    14641 atcgtcgagt agtgctgaga aggcaataac aattcttcgg taccaaattc cgggcatgct
    14701 tttattctac ctaaacagga gcaaccccga cagccctaag gaattctact cgctatttga
    14761 tacccagctc gataagctaa ttcgatttga aataccaacc cgtaccacac aaacatttga
    14821 agaagtagtg ctaaaattta ttttcgatgg cacttacctt ggcgaaaact attatttctt
    14881 tattcattat acccgtgaaa agcaggtagt tgattcaatt cttaaaactg gtttcaagta
    14941 tattgaatcg ttccataatt cagcagaaca aattattgat gacaagctcg actttcttta
    15001 taagcataac atttataaac cctacgggaa ttttatagtg gttataggga tttcgagaaa
    15061 tatttttgat aagtatgcac agctaattcg tagcaagggt attaatacct atatcgaaaa
    15121 tatattgtgc gacactgccc cggaatttga cgatgaggct gaggaatata ggtacactct
    15181 tccaacccag tacatcaagg gttatgttaa ctatgtaact ggtgaaaccg ttaaaaatcc
    15241 gagtttcaat ccggattatg attctacaaa ctttttgaat aatctgaact cgctctaatt
    15301 atttcagcct accgaagaac atagtaatgg ccaaccagta atcgttgctg gatgggttcg
    15361 atttccagag tgcgcttgaa ccgtgtttgc ctttatcggt gggacaaaat tgggttacca
    15421 ggttttgtcg tttgatagct tcaattaacc cctttacgga tgagcactct gcttttgatg
    15481 aaagcacgat tacaggaaca gaaatatcct taatttttga cgacacaaac tcaggtgtat
    15541 caaaatactc gcctggagaa aatgcaacaa cggctttggt gcgcaaatcc ttggcggttt
    15601 gaatcagagc aagcgatgct gagtatgaac taccccagag cacaaagggt aaattggttt
    15661 gccttttaac gtatgcaata gccgcctcaa tgtcgggtat ggcatcggtg tagttctggg
    15721 gcaagccctt ttcctttgcc cttttggctg tttcgttcac aacgccgtta acctcattgc
    15781 ccgatcgcaa atcaacagct atgcagttat aaccgaggtt aacaattttt ggagcaattt
    15841 caatatattc acccctacta tatcgggcct gatgtaaaag aataacgtat ggtttatcag
    15901 aggctaaaaa atacaaatcg gcagtaacct ctatgccatc ctttgctgga aacgatattt
    15961 tttgctgtgc catagtttca ttcaaattca gaatgaacgc aaagagtata gttaaccaag
    16021 cgtttttttt catggactaa aaggtttggt taatctcctc cgatacaatt gaaatcacgt
    16081 cattttctac acccacaaat cctccggcag gtatggcaaa gaatagttca gcgccatcgt
    16141 tcctgactat tttaactact ccaggttcaa gcgacgaaat aataggggca tgctttttca
    16201 gtattccaaa cgaacccttt gtgccgggta cttgaacgta gtaaacatcc tcaacttgga
    16261 aaaacttttc aacggagtag agctcgcaaa tcataaccta ctgttttttt gcttcggcaa
    16321 gcattcgttc gcctttctct attgcctcct caatggttcc aaccagatta aaagcagctt
    16381 cggggtattg gtcaacctcg ccatccataa tcatattgaa accctttata gtatccttaa
    16441 tgtcaaccat tactccttta atgccagtaa aggcctcggc cacgtggaaa ggttgcgaaa
    16501 ggaatcgctg aacacggcga gcacggtgaa ccaccagttt gtcgtcttcc gaaagttcgt
    16561 ccatacccag aatggcaatg atatcctgca actccttgta gcgttgaagc aggtttttaa
    16621 ccctttgagc cgtgttgtag tgttcatcgc ccaccacttc gggggtcaag atacgcgatg
    16681 tggaatcgag tgggttaacc gcgggataaa tccctagctc tgctatttta cggtcaagta
    16741 cagtggttgc atcaaggtgg ctgaatgtag ttgcaggcgc ggggtcggta aggtcatcgg
    16801 cgggaacgta gatggcctgt acggatgtaa tagaaccttt tttggttgag gtgatacgtt
    16861 cctgcattaa acccatttcg gtagccagcg tgggttggta gcctacagcc gaaggcatac
    16921 ggccaagcag tgccgatacc tcggagcctg cttgggtaaa gcggaaaata ttatcgataa
    16981 agaaaagtat atcgcgcccc tggccatcct ctccaccatc gcggaacgat tctgcaaccg
    17041 ttaatccgga aagggcaacc gtggcacgtg ccccgggagg ttcattcatc tgaccgaata
    17101 tcagagtggc ttgcgacttt tcaagttctt cggtatcaac taaactcaag tcccaccccc
    17161 cttttagcat cgattcctta aattcttttc cgtattttat tacatcggat tcaatcattt
    17221 ccctgagtag gtcgttccct tcacgggtgc gctcaccaac cccagcaaaa actgacatac
    17281 cgctataccc cttggcgata ttgttgatga gttccatgat gataaccgtt ttacctactc
    17341 ctgcaccacc aaataatcca attttacctc cctttgagta gggttcaagc aggtcaatta
    17401 ctttaattcc cgtatatagt atttcctttt gggtgcttag ctggtcaaat tttggtggtt
    17461 tgctgtgaac ctgatagtta ctttggcggt caacctgtgg aagaccatca atggtttcgc
    17521 caattacatt tagtaaacgg ccttttattt tgttgcccac tggcatggtt atcgacttgc
    17581 cagttgctcg tactttcatg ccgcgcgata ggccatcggt tgagtccata gcaatggtcc
    17641 taacagtact ttcaccaata tgttgctggc actccagaac cagtatggtg ttatcgggcc
    17701 gggtcaactc aagagcttcg taaattgagg gtaacttttc cttactggga aaggccacat
    17761 caacaactgg accgataatc tgaacaactt caccaaaatt atctgacata cttttgcttt
    17821 ttgcttactt caaatttatg aatttcccgt tgcaaattct aacaaaatca actataaatt
    17881 tactttaaag ttaaggctgc ttgtatcatt ttgtttatgt caacagccac ctcgttttgt
    17941 ccataggttt cagcaattga tgccagttcc tgtagggttt gagctgttag gcctatgtcg
    18001 tcctggtagt attgcttcat cctatcatcg agtgatacgt agtagcgaag tttttgttcg
    18061 ctaacctttg cataatccag ggtcaactta ttggcttttt gaacctcgtt cagcttgtag
    18121 taaccctcaa taatgccaaa cataaagaaa tcgtaaggga atttaggatg aggaagaagt
    18181 tccactgccc tgtcaagtac tttaagagca gaatcctttt taccctcctc aattagtttt
    18241 ttggccaggc gcgaaaagct gttacgaagt tttaaaacca tagcagtacg ttgcacgtta
    18301 tggtcaataa acacatcggg ctcatttatt ctgccccaac ggaatcggtt cattaaccta
    18361 tcgtaaagaa tatcgctatc aattcttgaa atattcaggt agtccgatga gttcgatttt
    18421 attggaacca gcctgtaggc aaagccttcg tgctgaaggt aatcggcaat gccaatatca
    18481 ccatcaccaa aaggtgaaac aaagtaaatg gggcgttccc aattattgtt tgcaatgata
    18541 tcaagaatgg ctaaggcgct tttagtaata tattgccctt tgagcgtcca ttccatggta
    18601 tcgacaatca agctggcgtc cttttccttt acggttccgc tcgacagcac tttagactta
    18661 tcaacagtta gctttatctt gttagtaggt aagtaatcga ggggcttttc gccaggtatt
    18721 ctatattttt ttgcaggatc atcgcttaaa acgaaatcca tgagctgttt tagctcaatc
    18781 ggttcgttta gccggtttat aatgtaaacc acatcgcggg ttcccattac atactggtcg
    18841 tgtttcatgg aaattggcac gggtgctgat tggtagcact tcattttcat ctggtcaata
    18901 taccagtcgg tacccagcaa gctaaggttg acaattctaa catcggtacg gataccttcc
    18961 acctcctgtg cataccaaag cgggaaagta tcgttatcgc cattggtgaa caggattgca
    19021 ttgggctcgc aggagtttag gtagttgtat gcaaagtcgc gtgcaatgta tcgaccggaa
    19081 cggtcgtgat cgtcccagtt ttcggttccc atgatcaccg gcacaaccag gcaaagggtt
    19141 gtagcagcaa ttgccgatgg tgtttcggaa agttttttac ggaaccactc ataaacagca
    19201 agaacaccaa gaccaatcca aatggtaaat gcgtagaatg aaccagcata ggcataatcg
    19261 cgctcacgcg gttgaagcgg gtattggttc aggtacataa cgatggctat tcccgtaagg
    19321 aagaacagca tgaatacaac ccaaaaattc ttcttatcct ttttaacctg aagctggaag
    19381 tatataccca gcaaacccaa tattaggggg aggaagtagt aagtattacg cgatgggtgg
    19441 cttttcatat cgtcggtcag gttgtcctgg tttccaagga aaatggcatc gataggtttg
    19501 ataccgctaa tccagtttcc gttttcaacc cctccatggc tctgaacgtc atcctgacgg
    19561 cccacaaagt tccacatgaa gtaacgaatg tacatgtgac caatctggta gcggaagaag
    19621 aataaaagat tttcgccaaa ggttggcaca tatcgggttt cggttccacc gtcacgggtg
    19681 ttcacctgaa cgggttttcc ctgtatatta gcccacttct tgtattctgc cacatggtcg
    19741 ggttggttgc tatacatacg tgggaaaaga gtcttgaacc tatcgtcaaa ctcgtacttt
    19801 gttttcaggt acccccttac gtacttaccg tcttcaggta tccatgtttc taagtcctta
    19861 ctgtcagtaa caggggcatt gtagtactgt ccgtagaaca atggtctatc gccatactgt
    19921 tcgcggttca ggtagctaat aagcgaaaag gcgttcgatg gattattctc attaatgggt
    19981 gtattggcat aggcccttat tagtatcatc atgtacgatg agtagccaat tagtattacg
    20041 gttacaccta ccagaacggt attaagaata accctacctt ttttatggct atagtaaata
    20101 ccataggcaa gggctccaaa aagtaaaaca aagtaaaaaa acaaacctga gttatagggt
    20161 aaaccgaatg agttaacaaa aagaaggtcg aaccatgagc caaacttaaa ggttccggga
    20221 ataattatgt atagaatact acctaaaata attgctgata ctgcaagggt tgttagaatc
    20281 ccgtttcggg tagtcttgaa ctttcggaag tagtaaataa atgctatggc cgggattgta
    20341 agtagattta gcaggtgaac gccaatcgat aaacccatga cgtaagctat aaaaattatc
    20401 catcgggttg agtgcgcctc gtcggcctgc tcctcccact tcaggattgc ccagaaaaca
    20461 aaagctgtaa ataacgacga catggcgtaa acctctgcct caacggcaga gaaccaaaag
    20521 gtatcggaaa aagtataggc aagggcgcca actgctccac tacccagaat ggcaattagc
    20581 tgaccagtgg aagttgtgta gctattacca acaattagct tgcgggcaag gtgggttata
    20641 gtccagaaaa gaaaaagtat ggtaaatgcg cttgccaaag ccgacatgga attaataagc
    20701 gcagcaacct ttgttacatc tggcgcaaag agagcaaaaa ggcgggcaat catcataaaa
    20761 agtggtgccc ctggggggtg accaacttcg agtttgaatg ccgtggcaat aaactcaccg
    20821 cagtcccaaa aacttgctgt gggttctatg gttaacaggt aaactattgc cgatattgcg
    20881 aacacaatcc atccgacaat attgttggct ctacggaaat tgctcatgat ttattttttt
    20941 attttgacaa ttgtttattg cagcttacaa gaaagcaaat gtagaatatt ttgttttacc
    21001 ctgattacca atttgcagtt tttaagcctt acgctaaaat tatccttttt tatatctgcc
    21061 tttcttgttg gtttttatcc taattcaata aaatactgga tgagctgtaa aatctgttag
    21121 gcaacctcta ctttaaaata atcgtaaaat ggactatgtt tgtatattgt aaattttaaa
    21181 aatatggatt ggaaggataa actaaatttt gcctactcaa ccaaccctga ctataagccc
    21241 aacgatgaag atgataatac gacccgggaa gttgtccctc catcgaaaca acggctaatt
    21301 gtaggaattg aacgcaagaa taggggaggg aagtgtgtta ccattgtgaa agggtttgtt
    21361 ggttccgacg atgaaatcga aaagttaggc aaaacactaa aaacaaggtg tggtgttggc
    21421 ggaaccgtaa aggatggcga aatcattatt caaggcgaaa taaaacaaaa agtggcctca
    21481 attttgcaag aactgggtta tagggttacc atttcaggat aggtttatgc gtactaaaat
    21541 tattatcata ggatttttgc tttttgcata tcggctatca ctagctgata cacttaccgt
    21601 tatgcagtat aacttgatgt attacgacaa ggaatacagc gactgtaatg ctacaaataa
    21661 caatgttgat gctaaggatg gctatctgcg cactattcta aaccatgtaa agcctgatat
    21721 cctttccgta aacgaggtaa acgatgccat tgccagcgtt gaacgaatta agacaaatac
    21781 ccttaattat ggtggcgaaa cccgctacaa acgagctaat tttagcggca gctttctggt
    21841 taatatgatt tactataatt ctgaaaagct ggagctgaag tcgcaggatg caatagccac
    21901 atcgccacgc gaaacaaacg tttataagtt ttaccttaaa acatcgggtt tggtaaatgg
    21961 cgacacaatt tttctttacc actttgttac ccatctcaaa gctggaagtt acgaatccga
    22021 tgcacagcag cgcgcagtag cggctaattc cataatgagc tacatatcaa ccaaaaacat
    22081 taaggggaac gtaatactct cgggcgatat gaacctatac agcgcctctg agggcgcatt
    22141 tgtggctttt actactccgg ttggttcaaa caacttccgc ttttacgacc ccctaaatgc
    22201 agttggcagc tggcactcga actatagcta tcgctatgtt catacacaat cgacccgaac
    22261 agttgctagc gagggctgct tttcagccgg agggctggat gaccgtttcg actttattct
    22321 ttcctcaaac gatattcttg ctggtaccag cggtattaaa ttttatagct ataaaaccct
    22381 tggccaggat gggaatcgat acaacggttc cattacatcg cccactaata caagcatccc
    22441 aacggatgtg gcaaatgcac tatacggcat gtccgatcac cttcccgttg tgatgaaggt
    22501 cacttattca tcggctccca ctcaagtaaa taccgagaaa aatcaggtaa gtattctgta
    22561 caataatccg gtttacaata cccttaacgt ccaaatcctt gaaaacacat caatcaaggc
    22621 aataaatgta tacaacctgt tgggtaattg cattcttaac ttaatccctg aaagcaatag
    22681 tcaaaaagtt gacgttgacc tttcaggtgc tcctactggt gttttactag ttgaggtaag
    22741 gttaaatgac ggtaggaaaa aaactataaa agtgctaaag caataaatga tgataatcaa
    22801 tatgtttaat aaaaaaatct acttttttac tttaatactg ataattttaa taaataattc
    22861 aaaagcacag tactatcaaa caggagtaga gccatttaaa accgattgga aaaagagaga
    22921 atacaaaaat atcaggttaa tatatcctgc cgaagccgat agcttagcca gacattacct
    22981 ttccgcttta gctaaatccg atagccttaa cgatgttgat tacaatctag ggacaaggaa
    23041 aatagatgtt gtgcttcacc caaacactgt tctatccaac ggatttgttg cctgggcccc
    23101 gcgcaggatg gaacttgtaa cccaacccga tgctgacgga gatgcattgc tttggcacaa
    23161 agccctttcc attcacgaga tgcgccatgt gaaacaaatg tatgcgctga atagcggatc
    23221 taccaaagtg ttatcgtggc tgtttggcca gcaggccgtt ggttttgctt catcgtttat
    23281 acacccatgg atttttgaag gcgatgccgt ttgggctgaa acaaattata catttagcgg
    23341 aaggggtcgc tcagcaactt tttttaacca ttactacgcc catttagtat caaagcaaaa
    23401 accttatact tacgataaat ggcttttggg ctcttacaag gattttattc caaaccatta
    23461 ccaatttggc tatcagcttg taagtttcat taaccaaaag tggggatcaa ctactattcc
    23521 caaaacatac aggtatgttg gtcgatatcc attcacgtta tttcctacct atctaagtct
    23581 gaaaaagttt acaggtcagt caaggaagag tatctaccaa aaagcgtttt catacaacga
    23641 ttcgctttgg cttgtagatt atactgaaca ttacggtaac gagttgccta aagctgtaaa
    23701 tgaggatttt aaaaatttaa tacaccccta tccgttaacc gacacaacaa caattgtata
    23761 tgtaacatcg cttagcgata caccctattt tgctgttttg gataaaagag ggggaataaa
    23821 aaagattgtg agtacaggca tactaattgg ccaaccatcg tacaacgaca gccttattgt
    23881 ttgggcggaa taccaacccc acgcgcgctg ggaatggatg agcagtagcg ccatcaaggt
    23941 ttataatatt aaaacaaagc ggttgaacac aataccgggt ggccgatacc attccccaat
    24001 tattgacgag gataggatta tctgcattga atatagctca aatggacaat acaagttggt
    24061 tggtataaat ttcctaggag agaaaagtac tttgttaaat tttgaaggag gatatgaagt
    24121 acagcaaatt tgtttagata ctaaggggaa aaatatttat ggcattgcgg taagcgaaac
    24181 cggcaaaaaa atatttaagg ttgattctgc attaactttc gatattgttt acaatgcagc
    24241 ttatcgcgat atcagaacac ttgcttgtga tggctcaaac ctatttttta cttttactga
    24301 cggttacact gaaagtatat ataaatatga tatacagaat aatatagtat ataaaataat
    24361 taacctacca tttaattcat cgtatccaac ctataaaaat catcagctat attatgccca
    24421 ctatacagca aatggctacc gaattgcttt tttagatgat attacaagta acagaaaaat
    24481 agtagataat atatcaccat ataatgaaat agttaaagta aatccaacaa cggttcacga
    24541 taattatggg ataattcaaa cagaaccaac cgaatataaa gggttaaata cattggtaaa
    24601 catacatagc tggtttccat tttacacaaa gccgctaaac gaccctgcca atattgagga
    24661 tgaaagcaac atttatcccg gaataacgtt actttcacaa aacttaacag gatcaaccct
    24721 ttttaatgct ggttatgggt atggcaaaac tcacttgtta tttgcggagg taacctacaa
    24781 tggactttgg cctgttttta gattaaatat tgagaaaacc gatgcaactg caagccttta
    24841 ccgagtaaca cagttttacc ctgaaaatag ggattactat aaaaaggcag agtttacttc
    24901 tattttacct tttactttag gtgaaagcat ttatagtaca tcattacaac tatataacaa
    24961 aaccatttat agcaacagtt atctataccg cgagagtatt gatgcatatc gttcaggctt
    25021 aattaccaat gagtttggtt ttacttttta ctcgttacga cgaatggccc atagggattt
    25081 acagcctaaa tcgggcttaa ttattaatac aggattactg acaacacctt gggattacgg
    25141 caacctaccg aatttgtggt atagtcaagc caaaatttac ttacctggat tgcacagaaa
    25201 tcatggattt ggaattacac tattagcaca aaaacagaac attaagtata tatacctaaa
    25261 taacaaggtt aattttcctg ctttttacac ttcaagacct tcgacacgat tcacaggtat
    25321 gtactttaat tacataatgc ccatagctta tcctgattta cccataagtt cacttgttta
    25381 tgtaaagcgt atatcgctta accttttcgc cgattttgca aagaacagct ataaaaccta
    25441 ccagcaatat gaattagtta cattaaccga taatttacag tccataggtt ttgatgccat
    25501 tgttgatttt catttattca gaacatggta tcctttcagg attaggttta ctcaggcatt
    25561 caatggttcc gattttaatc cttactcaaa tatagtttta actatcaatt tatattctac
    25621 ctttgccagg ccaaaattgt agttggtttt tcaatgttat ttggttaagg acttttgtca
    25681 tgttgagcga agcgaaacat ctcaaaacct agaaaaatag atatacttct gatttactca
    25741 ggatggtaat gtctgttttg atatctaaac aacattggtt attgggtaga aaatttgaat
    25801 gtctccataa tttgagtagt gttggattaa acctttctgg gttaattccg taataaaatt
    25861 tatgagcaag aacgagctaa aaaatcaaat ccggaaggct attaagtcgc ataggctatt
    25921 actggatcct ttcttggcaa gggaaagggc gagttatgta tttgcagaaa ttgaaagcat
    25981 ggagcagttc cagaaagcaa cgactgttct agcattttgg tcgttaccgg atgagataaa
    26041 tacacatgac tttgtaataa aatgggcagg cagcaagcgc atggttttgc ccgtagtggt
    26101 tggtgatgaa ttggagttga ggttattcaa cggagttgaa aacatggaga agagtggtgg
    26161 ttttggaata atggagccca aaactggcag tctagttaac cccgatgaga ttgattttgc
    26221 cataattcct ggtgttgcat tcgacagaaa tgggaatagg cttggcaggg ggaaaggtta
    26281 ctacgataga accctccctt tacttcgcaa tgcagttaaa gtaggcatag cctatgagtt
    26341 tcaaataatc gattcagttc cggtttcgga acacgacatt cctgttgacc ttgtggttag
    26401 caattaaaag ttgattttta tgactggagg atttaggaga gcacttacac tcgggttact
    26461 tattttaacc ctatcggtaa agatttttgc acaaattcct gatggttact acgccacggc
    26521 cgaagggaaa acaggtgctg agctaaaaac tgctttgtac aatatcataa agaaccatac
    26581 ggtaatatcg tacgatgggt tatggacagc ctttcagtac accgataaga aaagcaatgg
    26641 aaaggtatgg gacatgtact ccgataaacc cggccaaaca ccaccttacg agtttacttt
    26701 tgttactgac cagtgtggaa actatagttc cgagggcgat tgctataacc gtgagcattc
    26761 gtttcccaag agctggttta acgatgcatc tcccatgtat tccgacttgt ttcaccttta
    26821 ccctaccgat ggttatgtaa acaataggcg cagcaatttt atttttggtg aggttacatc
    26881 tgttacctgg acatcgctta atggaagcaa gttaggaacc tcttctgctt ccggagcaac
    26941 attaacagta tttgaaccca ttgacgagta taagggcgat tttgcccgta catactttta
    27001 catggcaacc cggtacgaaa acttaattgc aacttgggca agctacaata ccgaagccaa
    27061 agcaatactc gatggaaccg cctatcctgc atacaagcag tggtatatta ctttactgct
    27121 caaatggaat cagcaagacc cggtaagcca gaaggagatt gaccgtaata acgtagtgta
    27181 ttccagctac cagcataatc gcaacccgtt catcgatcat ccagagtatg cacagttaat
    27241 ttggggaagt acaacaccaa ttacattcac aagcacgcct attacttcag ctactgttgg
    27301 ggtgacttat acatataatg ttactgcaac cggtggaaat ggaacgccac tcaccatttc
    27361 ggcaacgcaa aagccaacat ggcttacttt gacagccaca ggtaatggta ctgcaaccct
    27421 aagcggtaca ccaggccagg aaaatgtagg tggcaatcag ataaccttaa aagctaccga
    27481 tggaattagt gagacacaac aaacatttac cataacagta agttcgcctg ttacccctat
    27541 tcagtttacc tcaacgccta taaccacggc agttgaaaat cagctttaca catataatgc
    27601 tactgcaact ggcggaaatg gagcaccatt aaccattacg gcaacacaaa agcctgcatg
    27661 gcttacatta acctcaaccg gaaatggtac agcaacccta agtggcaccc ccgatcacag
    27721 taatattgga aacagccagg tagttctggc tgcaaccgat ggaaccaacc agggtacaca
    27781 aagttttaca ataaatgtaa gtgccgccgg aaactcaaag cctttaataa ccaatgttaa
    27841 cataacccca gcgtcgcctg taacccgaca ggccataact atttccgcaa acataaccga
    27901 tagcgacggc agcgttgcaa aggttctctt aggttggggc gttactgttg gcaacttgag
    27961 taatacctac gaaatgcagt ttgagaatgg tgcatacagt gcacccattg cttcgcaaac
    28021 gagtccggga acaatctatt ttcagctagc ggcaattgat aatttacagg ataccgccta
    28081 ctacaacgga tcgtttacag taaatctcaa tcaagtacca accatttcta gcgtttcaca
    28141 taccccaaca aacccaactt cagccgatgc ggttactgta accgcaaacg taagcgaccc
    28201 tgagggccga ataggaaata ttttcctact ttggggtata acagaaagca gtttgatcaa
    28261 tcagctacag atgagtggca ctaacggagt gtatacctcg gttattccag caaatccatc
    28321 gcaaacaagc gtttactata gggttaaggc ttacgacgcc gaggggcaac agactttaag
    28381 cagtattagt agttacattg ttaatccagc taccgaaata cgaactgaaa ccagcaagca
    28441 atttttggtt tttcccaatc cgtttaatgc ttttatacaa gttgaatacc cttcagctga
    28501 aagctactcg gttactttta caaatcttat aggacaggta gtttatagta aaacgagagt
    28561 aaccggtaac ctacgaatag attgtagtga tttacaaagc ggaatctatg ttctaaccat
    28621 caaaagtgat actggcatag taactcaaaa aatgattaaa cgataagcat ttaatggctt
    28681 tatactagca accgagatgc agtttttagt aacccattaa aatgtgagta actatttcaa
    28741 tgtcgtttag ttaacaaaaa tatacatgcc tgtcattctg agcgcagcca aggattacta
    28801 atcgacccca tgagatgttt cgcttagctc aacatgaaag tagcttcacg aacaacgatt
    28861 aacgaacaaa gaacaacgct tcccgccctg taggggcgta atatctgtaa ccccacctgt
    28921 tagcgtgctt ctccatacgc gtgcattatt cgccctaaaa gggcaaatca tattagccct
    28981 gggttttaac ccagggtatt gaggtattca aaatattaag cgatgcacgc tatggtattc
    29041 caaagagcaa gggtgaatcg ttgcatcgca atgggaaagc agggcaaaga gctatttaaa
    29101 cgttctttct tgtcttgata caagaaagaa ccaaagaaaa tcaaggctga aaagcccgac
    29161 ccgatgggta ccccgtgggt ggaactgccg cgcgatacta ttcgccacgc ctgcagcgtg
    29221 gctcatttgg tatcgcttac tagacccacc gccacgttcc acccccgacc cattgccggg
    29281 tc
//
LOCUS       DAOSAL010000027        28100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_1238_length_28100_cov_47.733785, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000027 DAOSAL010000000
VERSION     DAOSAL010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 28100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28100
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1238_length_28100_cov_47.733785"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            300..863
                     /locus_tag="PK049_05540"
     CDS             300..863
                     /locus_tag="PK049_05540"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOK61053.1"
                     /translation="MSTGYKIQEQDGAYYLTFQIVGWVDLFTRKEYRDVVIDSLKFCQ
                     KNKGLNLFAYVIMSNHIHLLAQSQNGNLSGFIRDFKSYTSKRFLEIMQSGKESRSEWL
                     RVVFEYHGKLKSKQTFQLWTHENHAEHIYSQKFIEQKVHYIHHNPVRSGIVVNPEDYL
                     YSSARNYVGLDSVIGVITVDIRWKTYN"
     gene            complement(959..2395)
                     /locus_tag="PK049_05545"
     CDS             complement(959..2395)
                     /locus_tag="PK049_05545"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOK61054.1"
                     /translation="MKNIKYAVLIFSFFIISCESSNKNKILIKKPVIIAGKIENPKTE
                     LVSIDYQDIVKGSVHYSQVIDTLDGTFKFVFDLVHGQDLRFEFNNTFITLFAEPSDSL
                     FIKINTTLTDSKNSIVFSGSNKLINEEINKYLSHKKIPPFNAECEGKSVKEYHNNLKF
                     QIKNELLELDSFVKKFEPSAKFIKWAKNDIIYNNSNYLIDYKFYLTNNNLPLTESLFN
                     SELFPTDDEQALISSMFGVYLWNYAYDKYIQNNSIVKDLLSKGKTLEAYRLCINKIMT
                     EEKKGLIRDIIVFKVILSLKEKSLTNSIKLTKNIDYKIDNPVLASELELRLHNASNIG
                     KILKFNSRGIHSEVTQNVMDILKKESEGRTIYVDFWAIWCGACRAEIPSLIKLNEKIK
                     NKNIQILSFCFDSDRNAWNALIKEKKIPGIHYLLSKEQASILKSKLKIQGYPRYMIIK
                     NGMVIDSNSLRPSSKEKILNELLRISAM"
     gene            complement(2537..3145)
                     /locus_tag="PK049_05550"
     CDS             complement(2537..3145)
                     /locus_tag="PK049_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61055.1"
                     /translation="MTENELRDLLIEKLEGIVNFPYLIDTEVPIPYKHIYIPANDFTK
                     LEIWCFKQDITIYKILFDKTVKQKDSKITKNEETLVEVILEKDSGQNSRHTGIPFVIL
                     ELKKGQPNTHEILTYSQKAEMIKTIFPYCQFLFLIYGDISARTYRHGVNFDEVISLTN
                     PNDIKEIDNLKATLLKHFDIALTKLKQLTKSNYKRKENKSIP"
     gene            complement(3306..4184)
                     /locus_tag="PK049_05555"
     CDS             complement(3306..4184)
                     /locus_tag="PK049_05555"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOK61056.1"
                     /translation="MRTIVITGANSGIGYETAKYLAFKGNTIIAASRQKDETLKIIDN
                     LNLQCKKANSKGSVKFYHLDLNDLKSVKTFAEKVITDYPIIDTLICNAGIMNSPYKVT
                     EDGYEQQFQTNFLSHFYLTHLLIDTIIKSDNPKIINVCSASAEKGTINTIEELEKISK
                     ISEQEYNAMKSYREAKLAQQISVMQFARMQEYIKIKFSVIHPGIVNTNLFYRNSGTLY
                     KIAMLPFVYLGYAFGFFKTPKQGAKTTIFLSENDNYESGFYWNNTKQLTPNPISNDEN
                     YSKQLYEWTLKKIEKR"
     gene            complement(4181..4711)
                     /locus_tag="PK049_05560"
     CDS             complement(4181..4711)
                     /locus_tag="PK049_05560"
                     /inference="COORDINATES: protein motif:HMM:NF024055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOK61057.1"
                     /translation="MKTLIAYSTKTGNTEKIAKTINGICPFESDLLKIDNNLNIDISD
                     YELCIVGYWVENSKANNDTINFIKPIKNKKVLLFGTTGTDPNHPYIKSVLTKTENEFD
                     KSNTILGHFICQGEIAKSVIDNFEFLVNSQPENKMLLALLNIFKEQFPISIGHPNDAD
                     IKGAKDYFQNIFNSLK"
     gene            complement(5027..6109)
                     /locus_tag="PK049_05565"
     CDS             complement(5027..6109)
                     /locus_tag="PK049_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61058.1"
                     /translation="MKKYVFFLILMFAITMQLLASSNDTIPAFIGIWYTPKMNWYVNF
                     IQHKAIGKPLEVDFNTSSMMSVEGNFGIRRIGLRLGLNAQFENNAISKAYRWGGYLGF
                     KGYWLKIQRSNISGTLYWTGELPPGDYQSQADFNNSYFNIDILKNFKKKRYVDGNWQR
                     DVTDNSGFYWGFGYTSMGFPVEIATLTTEGNLPDDLKFGKPAYDSLYKIRSFNLSGGF
                     DMLRQLCQVGGRYGLIPGQPPMKLAVYASTEDRIGFGPGKLTSRSIQMAEALNPGRTV
                     TSSSFFNVNVHYFLSVGFRYYIKSGPSLIVFAAGYDFEGTAFTPFGANTRKKEDLGLD
                     FAGVLLNHGFTFKMYIAFDRRWNDME"
     gene            complement(6199..6459)
                     /locus_tag="PK049_05570"
     CDS             complement(6199..6459)
                     /locus_tag="PK049_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61059.1"
                     /translation="MGTPWVELPRDTIRHACSVTHVVSLTKPTAMFHPRPIVGSGFYA
                     FICFFHSKLSQGKRLGVRSFPTNNEQLTRITSFVILSVAKDL"
     gene            7312..10941
                     /gene="dnaE"
                     /locus_tag="PK049_05575"
     CDS             7312..10941
                     /gene="dnaE"
                     /locus_tag="PK049_05575"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010801691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOK61060.1"
                     /translation="MGQFVHLHVHSHYSILDGASSIDKLISRALELGMPGIALTDHGN
                     MFGVKEFLSKVSKVNGKVNDEIKQLKCDIENAEGEIERGGGNPAGLEELRLKLKEAQA
                     KLFKPIVGCETYIAKKSRFDRTDKEDRHNYHLILLAKNKEGYHNLMKLVSYGYTEGFY
                     YKPRIDKELLRKYSKGIIASSACLGGEIPQAILNGNIDEAERLIYEYKEIFGDDFYLE
                     LMLHKSGEPRIDYEVYENQLKVNEVLIELSKKTGVKCIATNDVHFVLADDASAHDILI
                     CLNTGKKLSETNRMRYTFQEYLKSEEEMLELFPNCPEAISNTMEIYHKVEVYDISSKP
                     IMPYFPLPGGFTDDNDYLRHLTYEGAKQRWGENLSDTIVERLEFELGVIARMGYPSYF
                     LIVWDFIKAARDMGVSVGPGRGSAAGSAVAYCLRITDIDPIKYDLLFERFLNPERVSM
                     PDIDIDFDEDGREEVLQYVVNKYGAKRVAHIVTFGTMAAKSSIKDVARTLELELPTAD
                     RLAKMVPERPNITLKKAFEEVPELEKETKSDNPLIRQTLKHAQVLEGTVRQTGVHACG
                     IIIGRDDLEEYIPLCTNSDAKLFVTQYEGTHVEDIGLLKMDFLGLKTLSIIKDTLQLI
                     EQSTGKKVDIDTIPLDDSKTFELFSRGETTAIFQFESPGMKKYMRALQPNRFEDLIAM
                     NALYRPGPMDYIPSFVNRKHGREPIVYDIPEMEEVLKDTYGITVYQEQVMLLSQKLAG
                     FTKGQADALRKAMGKKLKKVMDELKEKFIEGCLSKGYDKKIVEKIWSDWESFAEYAFN
                     KSHSTCYAYVAYQTAYLKAHFPSQFMAAVLSRNLSDIKKITTFMDECRRMGIQVLGPD
                     VNESQIKFAVMPNGDIRFGLGAIKGMGENAAQNIIEIRAKGGKYKNIFDFFERVNLQT
                     VNKKNLEVLAVAGAFDNLIDFDRSVLLAVDAKGVSYLEQLMRYGIKVQSEKNSTQQSL
                     FGSVPGFEVPKPAPPKADPWPTIEKLNKEKEVIGIYLSAHPLDEFRFEIESLCNVSIA
                     DLKSNMEQYRDRDVTIAGMTIASRIDTAKNGKQYGRITLEDYTDSIDIMLFGKDFESY
                     RNFCFNGYYIMVRGKVQPKAYKPEELELHIKSINMLYDVREKMIKSITLNLPLDEIND
                     IFIDDILKYVQRDKANITLKFKVYDPEYQVSVDLFSRAYRVNITQDFIDYLNDSEINY
                     KVAME"
     gene            11016..11333
                     /gene="trxA"
                     /locus_tag="PK049_05580"
     CDS             11016..11333
                     /gene="trxA"
                     /locus_tag="PK049_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOK61061.1"
                     /translation="MALAVTDANFDELVLNSGKPAMVDFWAEWCGPCRMIAPYVEQMA
                     EEYKDKAVVAKVDVDSNPGIAARYGIRNIPTVLFFKNGQMVDKQVGAVPKNALVAKLE
                     ALL"
     gene            11399..11974
                     /locus_tag="PK049_05585"
     CDS             11399..11974
                     /locus_tag="PK049_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOK61062.1"
                     /translation="MRICVYCASSSKVDAKYFEATKVLAKDLVAKNVTIVYGGGSHGL
                     MGCIADTALEHGGKVIGVLPRFMEKVEWGHRNLTQLILVKDMHERKKLLIQDVDAVVA
                     LPGGCGTLEELMEVITLKRLGKFTKPIIILNTDNFYYHLKEMLQKMIDERFMRDEHNA
                     IWQFVDNPEEVIPAILNAPHWDSDAINFAAV"
     gene            complement(12074..>12909)
                     /gene="ribD"
                     /locus_tag="PK049_05590"
     CDS             complement(12074..>12909)
                     /gene="ribD"
                     /locus_tag="PK049_05590"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOK61063.1"
                     /translation="YGKTPPCTDRIVEYEIPRVVIATTDPNPKVAGRGIEILRQNGKA
                     VTVGILENEAIELNRRFITYHTKNRPYVILKWAQTMDGFIDKKREPNEPVGSNWITNE
                     LARSLVHRWRSEEQAVLVGTNTVEKDNPQLNVRNWSGRSPIRVVVDRKLRLPLNSNVF
                     DGSQPTLLVTGNNSSALARKTEFSGIDNLEIITIDFVKGVEAQILKELAEREILSVII
                     EGGAMLLNSFIQKNLWDEARMFVGNKFFGEGVKAPTLKGKMVSYDEIGDSRLFVYRNE
                     F"
     assembly_gap    12910..13009
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13010..13311)
                     /locus_tag="PK049_05595"
     CDS             complement(<13010..13311)
                     /locus_tag="PK049_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012056136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOK61064.1"
                     /translation="MENADVHRKYMARCLELALGGQGNVAPNPMVGAVIVHNNQIIGE
                     GFHAMYGQAHAEVNAINSVQNKELLKESTLYVSLEPCSHYGKTPPCTDRIVEYEIPR"
     gene            13325..14215
                     /gene="prmC"
                     /locus_tag="PK049_05600"
     CDS             13325..14215
                     /gene="prmC"
                     /locus_tag="PK049_05600"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOK61065.1"
                     /translation="MFYTKLSNFMEHITLSGLLNHVSRSLSGSFNENELHTIKKYIAE
                     AVLHVPYHQVFINKHHEVKHEDAQEVERIISELISGKPLQYAIGLAYFSDLVLKVTPS
                     VLIPRPETEELVSLIVKENRIVAPRILDVGTGSGCIALALAKHIPDASVAAIDISTDA
                     LDVARQNAKSNNLNVNFFQGDILQPTVLDGYTFDIIVSNPPYIRDSEKVLMNRNVLDF
                     EPHIALFVPDSDPLKFYRAIALHSVRRLETMGWLWFEINEALGSEIVSLLKSLGFGNV
                     LLIDDFRGKPRFVKAQKCNP"
     gene            14212..14703
                     /locus_tag="PK049_05605"
     CDS             14212..14703
                     /locus_tag="PK049_05605"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOK61066.1"
                     /translation="MIKGENHKALTPQKAMERLQYLCARQEKCTHDLVQKLKQWGFAQ
                     AEIDKIVGKLSSDGYVDDNRFARLYVREKSRINKWGPIKLRAMLVSKGIAKDIIDQAL
                     NELRYAENPVNLFDLLNKKMPQVKAKNRFDLRNKLIRFGASRGFALDEVIDTVNRLLG
                     AEE"
     gene            complement(14712..16880)
                     /locus_tag="PK049_05610"
     CDS             complement(14712..16880)
                     /locus_tag="PK049_05610"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOK61067.1"
                     /translation="MRANLLVFLLFVQSAFSQSINHWETAIYPSDNWRYRVNETATPT
                     GWNLSNFDDSQWSLGQGGFGYGDNDDATTVPNCISVSIRTTFNVSDINEIAMAVLSVD
                     YDDAFIAWINGVEIARSAGLQGVNVNWDTPSSENREAVMYTGGRPTDFLIKKDILASA
                     LRNGNNVLAIEVHNTSSGSSDLTIIPYLSVGITVSNSYYQLTPDWFNPPVTALSSNLP
                     IVIIDTHGQTVQSDNEIVATMKVVDKQSPPNSIADNDFTYNGTIHLEYRGQSSLWNDW
                     PKKSYNVELINALGENIDSALMGMPPGNDWALYGPYNDKSLIRNALAYEIGRRMGRYA
                     PRTAFCELILNEQYAGLYLLIEKVRRDKNRVNIAKLTQNDISGDDLTGGYIVKIDKGD
                     FDEFGWASPYTHNGKPINFLWHYPKPENILPVQKNYIRNYITTFENTLNSSYWNDPFL
                     GYYRYVNMASAVDYFLVNELSKNIDAYRISTFLHKDKDSKGGKLTFGPMWDYDLTFGN
                     ANYYSGDSPQGWVFKSISTDDYYQPPFWWDKFTTDDKFNNMARCRWEELKSSGLLTRT
                     GVHAIIDSMTTLIQDARIRNYNTFNSLGVWVWPNAFVGSTYEQEVNYLKNFVSSRLLW
                     LDANLPGVCKDEPNPIMISAIPNPFNEYFDLRVILPEVGQITLKLTSISGSVVWQMSE
                     NATLTSFVYRYQAQDLQKGIYILTAYLNGKYLGAVKVIKG"
     gene            complement(17095..18171)
                     /locus_tag="PK049_05615"
     CDS             complement(17095..18171)
                     /locus_tag="PK049_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOK61068.1"
                     /translation="MSKIIYGIQQVGIGVSNLTEAWKWYKEHLGFDIRIFEDNTVAEL
                     MLPYTGGQPQQRHAALALNMQGGGGLEIWQYKGRTPLMPEFQVQLGDHGIFAAKIKCP
                     DIHKAFNYYETKGIPVSQLTIRPNGEEHFFIKDPFGNFFEIYSHSYIFKNEKKPVSGI
                     GGAIIGVSNIDKALKVYSDILGYDTAIYDKTGTFDDLTELPGGKGTFRRMLLTHSDTR
                     KGSFSNFFGISEIELVQALDREPNKIFKNRFWGDLGFIHLCFDMKNMNDLEKECVEKG
                     FPFTVNSNVKHNDKGSFDMGEAAGHFSYIEDPDSTLIEFVETHRLPLIKPLGIQLNMD
                     KRDPFKPIPNWITNLLSLKRVKNL"
     gene            complement(18177..18974)
                     /locus_tag="PK049_05620"
     CDS             complement(18177..18974)
                     /locus_tag="PK049_05620"
                     /inference="COORDINATES: protein motif:HMM:NF004825.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOK61069.1"
                     /translation="MISFKNRLVWITGASSGIGKELALQLAEEGAHLIISSNDAQELS
                     HVADECREFTSFCQEYPFDLSNPEEVKTTAEAIINTYEQIYLLINNGGISQRSLVKDT
                     PVDIDRKIMEIDYFSYIIITKAVLPGMIETGEGFIAATSSISGKFGFPLRSSYAAAKH
                     AIQGFFETLRAEMKPHNISVTIAYPGRIQTNISLRAIDEKGKEHGVMDPGQLNGLSAK
                     ECARQYIRAIKKRKPEVYIGKSEILMVHIKRLFPSIFFRIVTKIKPT"
     gene            complement(19063..19521)
                     /locus_tag="PK049_05625"
     CDS             complement(19063..19521)
                     /locus_tag="PK049_05625"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HOK61070.1"
                     /translation="MKLFLTLLISLVSLVSFAQTNVVEREGKYYDSNGKLYSGRYTSY
                     YDNGNIKVEALITNGLPDGEMKLYYPTGKIQEIRTYSLGEKNGRWEKWNEMGIKVSEA
                     NFVKGKKHGKWYVWDDNGTLRYDMTYTDGEKSGTWIIYDENGKETSRKEY"
     gene            complement(19622..20965)
                     /locus_tag="PK049_05630"
     CDS             complement(19622..20965)
                     /locus_tag="PK049_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61071.1"
                     /translation="MNRKKSNLLLIAVALLGLMVTSCDKNDDDTPKGTKKTVSGEISQ
                     NTLWEAKDTIILDGYVYVKSGATLTIEPGTLIKGLSDSKACLIVERGARIVANGTAEK
                     PIIFTSDKPVGERKPGDWAGIIICGKAPINQTGGEAEIEGGTGAVYGGTDPNDNSGIL
                     RYVRIEFAGYEVSNGNEINGLTMGGVGRGTTIEYVQVSFGRDDSFEWFGGTVNTKYLI
                     SYRGGDDDFDTDNGFSGNVQFGLILRGPEEADKSDASNGFESDNDANGSSNSPLTSAK
                     FSNITILGPYATKTQANVNPNHKFGLRLRRNTRLNLYNSIIAGFNTGISLEGPSMELY
                     NAASGPEIKNCLMAGMVKNFNAKTDGTSLTQAQYETMFNAGNNTLYAENTQLQLTNAW
                     NWGNVNAMPQSGSPALTGASFTGLTGFETVTFRGAFGTQNWAQGWTNWDPVNTNY"
     gene            complement(21153..21494)
                     /locus_tag="PK049_05635"
     CDS             complement(21153..21494)
                     /locus_tag="PK049_05635"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOK61072.1"
                     /translation="MKKILSVLIVSMLALNLIAANEDAKKESAEATSNMVSTISGKVV
                     DKTTGEALAGVAVKLDENTVVYTDFEGNFTINLTQPKAKLCVSLISYAPAEVEVKKSS
                     KEVKIELNTVE"
     gene            complement(21672..24482)
                     /locus_tag="PK049_05640"
     CDS             complement(21672..24482)
                     /locus_tag="PK049_05640"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOK61073.1"
                     /translation="MVKTNNKIVLLLLSGFFVVFEAWAQKGSIEGVVYDRSHNETLVG
                     ANVIIEGTTTGTITDFDGRFTLSNLNPGTYNVLVSFISYQPVILNNIKVEPNKSTVVK
                     VELEEITTAIEGVTVTAARKTNTEVAMLSTIKASLTVASGISAQQITRSQDRDASEVI
                     RRVPGVSIIDDRFVVVRGLNQRYNNAWINNASTPSSEADQKAFSFDVIPSAMIDNMVI
                     YKSPSPELPADFTGGFIKISTKNMPDENFLSIGYATGFNTSTNYGNFKQITKSKTDWL
                     GFDDGTRNLPADFPSTLKGLTNSQLAGLGQKLNTSWAPQSVQAVPDQKFNLSFGRKWQ
                     KGNKRLGNITSLSYSNTFETEEVNHSEYQVQLVPGQEPPFNHNYTDSIFTRKVKIGLL
                     HNWSFFTGKGSKIEFRNLFNLIGKSNAIIRNGLNGHEGFTIRSYQDNFQNRTTYSSQL
                     GGEHRWGDNQKNKLDWVTGYALSYRNEPDLKQLRTTLQDEPLIPHYNEYYAAVGITPS
                     VSDAGRVYMKLVEHIASLGVNYERKLKIGNWEPTLKSGTYGEFKARNFDARILGFAKN
                     SSYTESVWLPIDQIFSKENINTSPDGFLLRESTSKSDSYNVSSILGSAYIALNTNITK
                     KLSFYGGLRAEWMNQILNSYDRYGRPVKVEDDGIDIFPSANITYNFNEKNLIRLAAGI
                     SINRPEYREIAPFYFYNFTDEAEFVGNTNLKNCLIHNYDLRWELYPNTGETFSIGLFY
                     KKFNGPIELVFKPAGARPLFTYNNAEGAYSFGVEVDLRKSLQAIPLLKDFYMVLNASY
                     IHSKVIFPEGSIFRNRPMQGQSPYVVNAGIFYQNDKNNLSVSVQYNVIGDRILAVGIP
                     FQNQDQDIPDYYEKHQHLVDITVSKEFGKKVEIKAGVKNLLNQKYNTYQPFIGSNNND
                     MTMNNKLYNQGITFSLGANLRF"
     gene            24668..25360
                     /locus_tag="PK049_05645"
     CDS             24668..25360
                     /locus_tag="PK049_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOK61074.1"
                     /translation="MNVSRYRILIVDDEPDIVEFLSYNLKKEGFQIQTASNGREAIER
                     AIDFRPHLILLDVMMPDMDGIETCEQLRKQPSTSNTLIAFLTARSEDYSQIAGFDAGG
                     DDYITKPIKPKVLISRIKALLKRSGTLIDDAESPDTIPAEGIYIDKERYSVYVNGNEL
                     SLPKKEFELLALLYSKPQKVFTRDEIYQAVWGDDIVVGDRTIDVHIRKLREKLGDDYI
                     KTVKGVGYKFTS"
     gene            25495..25902
                     /locus_tag="PK049_05650"
     CDS             25495..25902
                     /locus_tag="PK049_05650"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HOK61075.1"
                     /translation="MQIAIWKPEYSVGVKSIDEDHQKLFDLLNQLFESMTKGKGKEII
                     NDIISELERYAVFHFGREESYFRVTNYPFALQHIKEHQYFKQKVAELKKDVALNKGMV
                     APDVLGFLSDWLKNHIAKSDKAYEDHLKKHAVI"
     gene            complement(26036..27514)
                     /locus_tag="PK049_05655"
     CDS             complement(26036..27514)
                     /locus_tag="PK049_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61076.1"
                     /translation="MNVLRFIVLMGTFALALSFAGNDLVNFIGVSMAGLKAYQIHAAN
                     PDIAPESLRMTELAGEVSTNFMYLFIAGLIMVITLWTSRKARTVTDTEVGLARQDAGM
                     ERFGSTQFSRIIVRIARRFAEHVDKVTPRFIRKLVAKQFDTTKAPRYSNPKDAPSFDL
                     VRASVNLVMASILISSATALKLPLSTTYVTFMVAMGTSLSDKAWGRESAVYRITGVIT
                     VIAGWFFTAFVTFTIAFGIATALYFGKAPALIILLAIAIGLLIKSHLLHKKKTKQLTD
                     EEIVDEVSIVNRCTNELRKYLKQSIKVYNRTIDGLTTENRRQLKEVNEEVEALNMEAK
                     KLKYNVYYVLKQLEADSIETGHYYVQVIDYLRELAHSLEFVSRPSLQHVENQHKGLTR
                     EQAAELKDLSNQLSDLFDDILEMIKQNDYTRVPDAIKKQQDILEDLNKIRKKQVKRIK
                     QGETGTRNSVLYLGLLNEIKNLLLHTINILKAQRDFILNNIE"
     gene            complement(27579..>28100)
                     /locus_tag="PK049_05660"
     CDS             complement(27579..>28100)
                     /locus_tag="PK049_05660"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOK61077.1"
                     /translation="GSMFSSGMMEVARKGVFRPENFYFTEMMVIFLAVMITDVIVLDF
                     FNTFGLPTSTTVSLVFELLGSAVAVSIFKIMNTEGETISNLGNYINSGKALAIISGIL
                     SSVVVAFVVGMVVQYIARLLFSFNYQKNLKFVGGIWGGIAMTAITYFIVMKGVKDTTL
                     ISKETIIYIRSFI"
BASE COUNT         7943 a         5623 c         5748 g         8686 t
ORIGIN      
        1 aatattttca caaatcatca taagaccttt tattttcttg caaaccattt tccctgataa
       61 tacatgggaa cgcatgcgtc tagaggttca cccccgtgtt cggcggcggt tgcaccgccg
      121 tcgggctatc agtagcaggc tgtgcctgtt ttttataatt caagtacagg caaagccata
      181 agcataatga gcctaagcct aacgttacac tttagccaga ataggttaac aaaatctaag
      241 ttacacgtaa aagttattgc tgaatttttg tagatttgtt atagcaacag accggatcaa
      301 tgtccacagg ctacaagata caggagcagg atggcgcgta ctacctaacg tttcagatag
      361 tgggatgggt ggatctgttc acccgcaagg agtaccgcga tgtggttatc gatagcctaa
      421 agttctgcca aaagaataag gggctaaacc tattcgcgta cgtaatcatg tcgaaccaca
      481 ttcacctgct ggcgcagagc caaaatggta atttaagtgg gtttatccgt gattttaaga
      541 gctacaccag caaacggttc ctcgaaatca tgcaaagtgg aaaggaaagc cggagcgaat
      601 ggcttcgggt ggtatttgag taccacggca agctaaaaag caagcaaacc ttccagcttt
      661 ggacgcacga gaaccatgcc gagcatattt acagccaaaa gtttatagag cagaaggttc
      721 actatatcca ccacaaccca gtacgcagcg gcattgtagt aaacccagag gattacctgt
      781 actccagcgc acgcaactac gtagggttag attcagtaat aggtgtaatt accgtagaca
      841 tacggtggaa aacgtacaat tagctatatt taccgggcag tgcccgaata gatagattcg
      901 actgaggcgt atagcctcag ccgaacgggg ggcgggtcaa ttctaaagct gatataattt
      961 acatggcgct aattctcaat aactcattaa gtattttttc ttttgatgat ggcctaaggg
     1021 agttactgtc tattaccata ccattcttta taatcatgta ccttggatat ccttgaattt
     1081 taagttttga ttttaatatg gaggcctgct ctttgctaag caaataatga attccaggaa
     1141 tttttttctc ttttattaaa gcattccatg catttctatc agagtcgaag cagaatgata
     1201 aaatttgaat attcttattt ttgatttttt catttaattt gattaaactt ggaatctcag
     1261 ccctacaagc tccgcaccaa attgcccaaa aatcaacata aatagttctt ccttctgact
     1321 cttttttaag aatatccata acattttggg taacttcaga atgaattccc ctagaattaa
     1381 attttagaat tttgccgata tttgatgcgt tatgtaaacg cagttcaagt tctgaagcta
     1441 atacaggatt atcgatttta tagtcaatgt ttttggttaa ctttattgaa ttagttaacg
     1501 atttttcttt aagcgataaa attaccttaa atactataat gtccctaatt aaaccttttt
     1561 tctcttccgt cataatttta ttaatgcata gtctgtatgc ttcaagagtt tttcctttac
     1621 ttagtaaatc cttaactatt gaattatttt gaatatattt atcgtaagca taattccaaa
     1681 gataaactcc aaacatcgat gatattaagg cctgttcatc atcagtaggg aacaattcgg
     1741 aattaaatag actttccgtt aaaggtagat tattattagt cagataaaac ttatagtcaa
     1801 ttaagtagtt tgagttattg taaataatat cattctttgc ccatttaata aattttgctg
     1861 atggttcaaa cttttttaca aaactatcca attctaataa ttcattttta atctgaaatt
     1921 ttaaattatt atggtattct tttacactct taccttcaca ttcggcgtta aatggcggaa
     1981 ttttcttgtg tgataaatac ttattaattt cttcattaat taatttattt gaaccagaaa
     2041 agactatgct gtttttacta tcagttaatg ttgtattaat tttgataaat agactatcgg
     2101 atggctcagc aaataaagta atgaatgtat tattgaattc aaatctcaaa tcttgtccat
     2161 ggactaaatc aaaaacaaat ttaaaagttc catccaaagt atcaataacc tgggaataat
     2221 gaaccgaccc tttcacgatg tcttgataat caatactaac caattcagtt ttaggattct
     2281 caattttccc agcaattatc actggctttt taattagtat cttattctta ttagatgatt
     2341 cacaactaat aataaagaat gaaaagatta aaacagcata ctttatattt ttcatttgat
     2401 ataattttaa ataacttctg caaagttaat gtttttggtc attatgagtt tgagcaactt
     2461 tcttatgtaa aaccacacta aattattaat aactttcgta tctatttaaa aagccttaaa
     2521 taggctaaat caccttttag gggatgcttt tattttcttt tcgtttgtag ttacttttgg
     2581 tcaattgttt aagttttgtt aatgcaatgt caaagtgttt caataaagtc gcttttaaat
     2641 tatctatttc ttttatatca ttggggtttg taagagaaat cacttcgtca aaattaactc
     2701 cgtgtctata agtcctagca gaaatatctc cataaattaa aaacagaaat tgacaatatg
     2761 ggaaaattgt ttttatcatt tctgcttttt gagaataagt caatatttcg tgtgtatttg
     2821 gttgcccttt tttaagttcg agaataacaa atggaattcc tgtatggcgt gaattttgac
     2881 cactgtcttt ttcaagaatt acttctacta atgtttcttc gttctttgta attttactgt
     2941 ctttttgttt aactgttttg tcaaacaaga ttttgtatat cgttatatcc tgtttaaaac
     3001 accaaatttc aagtttagta aagtcatttg ctggaatgta aatatgtttg taaggaatag
     3061 ggacttcggt atcaattaaa tacgggaaat taacaattcc ttctagtttt tcaattaata
     3121 agtctctaag ttcgttttct gtcatatttc tgtatgtttg actattcgaa agtagcagct
     3181 aacggaaaat gctaaacgca ggtgcaggat ttgaaagtat aatgtttcat ttacaggaaa
     3241 atgtaaattc ttattccaat gtttcaagtt acgcacaatg cctgcacttg tcgttttagc
     3301 atatgttagc gtttttctat cttttttaat gtccattcat acaattgttt agaataattt
     3361 tcatcgttcg aaatcggatt tggcgtaagt tgtttggtat tattccaata aaatccgctt
     3421 tcataattat cattttctga caaaaatatc gttgtttttg caccttgttt tggggttttg
     3481 aaaaatccaa aagcatatcc caaataaaca aagggtagca ttgcaatttt atataaagtt
     3541 cccgaatttc gataaaataa gttggtattt acaattccgg gatgtataac cgaaaactta
     3601 attttaatat attcctgcat tcgtgcaaac tgcataacac tgatttgttg ggctaatttt
     3661 gcttctctat acgatttcat tgcattgtat tcctgctcag aaattttgct tattttttct
     3721 aattcttcaa tcgtatttat tgtgcctttt tcggctgatg ccgaacaaac atttataatt
     3781 tttggattat cggatttaat aatcgtatct atcaataaat gtgttaaata gaaatgactt
     3841 aaaaaattgg tttgaaactg ctgttcgtaa ccgtcttcgg tcactttata tggtgaattc
     3901 ataattccag cattacaaat tagcgtatca attatcggat aatcagtaat tactttctct
     3961 gcaaaagttt ttaccgattt taaatcgttc aaatctaaat gatagaattt gaccgagcct
     4021 tttgaatttg cttttttaca ttgtaaattc aagttatcta taatttttaa tgtctcgtct
     4081 ttttgtcttg aagcggcaat aatggtattt cctttaaaag cgagatattt tgctgtttcg
     4141 taaccaattc cgctatttgc accggtaatt acaatagttc tcattttagc gaattaaaaa
     4201 tattttgaaa ataatctttt gcacctttta tgtctgcatc atttgggtgt ccgattgaaa
     4261 ttggaaattg ttctttaaag atgtttaata gagccaaaag cattttgttt tcgggttgcg
     4321 aatttacaag aaattcaaaa ttatcaatta ccgattttgc aatttcgccc tgacaaataa
     4381 agtgtcctaa aatagtatta gatttatcaa attcgttttc ggtttttgtc aaaacacttt
     4441 ttatgtaagg gtggtttggg tcagttccag tagttccaaa taatagaact tttttatttt
     4501 taattggttt gataaaatta atcgtatcat tgttggcttt gctgttttct acccaatatc
     4561 caacaataca taattcataa tcagaaatat ctatgtttaa attgttatct atttttagca
     4621 aatcactttc aaacggacaa attccgttta tcgtttttgc aattttttcg gtatttcctg
     4681 tttttgtaga atatgcaatt aatgttttca tctaatcatg tttttagtaa acgctaactc
     4741 gtttataacc ttaccaaaag taattatata catctatttt gggtgtataa ccgtactttt
     4801 tgtaaggttt atctaaacaa aagtaaaata ttttcacaaa tcatcaaacg agtttttaaa
     4861 tttcttcaga ccattttccc taacacttga agcttttcgt ttatgattga tagtgagttt
     4921 catagaacgc agattacgct gaattattgg atttacacag gttaaattaa ccaaacacta
     4981 aacaccaaac actatctccc ctgtacactg cacactgcac actaccttat tccatgtcgt
     5041 tccaacgcct atcaaaggct atgtacattt taaaggtaaa tccgtggttt agcagaacac
     5101 cagcaaaatc cagccccaaa tcttcctttt ttcgagtgtt tgctccaaag ggtgtaaacg
     5161 cggttccctc aaagtcgtat cctgctgcaa aaactattag cgatgggccc gatttaatgt
     5221 aatacctaaa acctaccgag aggaagtagt gcacattcac attaaaaaac gatgaagagg
     5281 taaccgtgcg tcctggattc agggcctcag ccatttgtat acttcttgag gtaagcttac
     5341 cgggcccaaa accaatcctg tcctcagttg atgcataaac tgccagtttc attggaggtt
     5401 ggcctggtat caggccatat cggcctccaa cctggcatag ctgccttagc atatcaaaac
     5461 caccacttaa gttgaacgat ctgattttat agagcgagtc gtatgcaggc ttgccaaact
     5521 ttaggtcgtc agggaggtta ccctcggtgg ttagcgtggc aatctctacg ggaaatccca
     5581 ttgatgtata tccaaatccc cagtaaaatc cagaattgtc agtgacatca cgttgccaat
     5641 tcccatcaac gtaccttttc tttttaaaat ttttcaggat atctatattg aaataggaat
     5701 tgttaaagtc agcctgactc tggtaatcgc caggtggtaa ctcaccagtc cagtatagcg
     5761 tacccgagat gttggagcgt tgaatcttca accagtagcc cttgaaccca aggtatcccc
     5821 cccaacggta tgctttgcta attgcgttat tctcgaactg cgcatttaaa cccagtcgca
     5881 aaccaatacg ccgtatgcca aaattcccct caacgctcat catcgatgag gtgttgaaat
     5941 caacctcaag gggtttacct atggccttat gctgaatgaa attaacatac cagttcattt
     6001 tgggagtata ccaaatccca ataaaggctg gtattgtatc gttagagctg gctaagagtt
     6061 gcatcgtaat tgcaaacatc aggattagaa aaaaaacata ctttttcatc cgtacaaaat
     6121 atttatttat taattcaatg ttgtttagtt aaggttattt gtcatgttgc actcaagcga
     6181 aacatctcaa tgggtcgatt agagatcctt cgctacgctc aggatgacaa atgaggttat
     6241 tcgtgtaagt tgctcgttgt tcgtgggaaa cgacctcaca cccagtcgtt ttccctgaga
     6301 gagtttggag tggaagaagc aaataaaggc ataaaagcca gacccgacaa tgggtcgggg
     6361 gtggaacatg gcggtgggct tagtaagcga taccacatga gtcacgctgc aggcgtggcg
     6421 aattgtatcg cgtggcagtt ccacccacgg ggtacccatc gggtcgggct tttcagcctt
     6481 gattttcttt ggttctttct tgtatcaaga caagaaagaa cgtttaaata gctctttgta
     6541 atgcttttcc tttgcgatgc aaggcttcag tgttgctctt tggaaaaggt gttgcgtgca
     6601 tcgccttaca tatgggagta ttcatagggt tacaaatatt tcgcccctac ggggctggaa
     6661 gcgttgttcg ctatttgtga atcgatgttc gagaatatca cgtcacacgc tctgtgtaac
     6721 accgtgttac attgtgtaac tctatgatac ttgctgttac tcgttgttcg tgaaactatt
     6781 gtcatattga gcgcaagcga aacatctcat ggggccgatt agagatcctt cgctccccgc
     6841 tagggcggga caggccgctc aggatgacaa gcatgtttat gtttgttaac taaactacat
     6901 tgattattaa ttactaactc gttactctct tgttttcaac aaactttatc gagcggatgg
     6961 aacacccata ctttgttcat ccgtgtttgt ataagttgcc ttacatgggt gtttcatggt
     7021 gttttttatt aaaggtcaat aataattgag aaggtagaac atgaaatgcc tttttaaaac
     7081 gtcatcaatt tagctgtttt tcacacttgg ttactaatat ttcttcgttt tcagccattt
     7141 ttttattgat acatagtaat atgggtttaa ctgcataaaa ttgttgataa aaattcaata
     7201 attacacttt tccattcacg ggtaagtggg gttatttggt taacttcgca tgtaaggttt
     7261 aagtgctatt ttgcaagtaa tcaggtagat attaattcaa tacacaggta aatgggccag
     7321 tttgttcatc ttcatgttca tagccactac tcaatcctcg atggggcatc gagtattgac
     7381 aaactaattt cacgtgcctt ggagctggga atgcccggca ttgcccttac tgaccatggt
     7441 aacatgtttg gggtaaagga gttcctatca aaggtaagca aggtaaatgg caaggtaaac
     7501 gacgaaataa aacagcttaa gtgcgatatt gagaacgccg aaggtgaaat tgaacgtggc
     7561 ggaggtaacc ctgccgggtt ggaggagctt aggttgaaac ttaaggaggc acaagccaag
     7621 ctttttaagc ccattgttgg ctgtgaaaca tacattgcca aaaaaagccg tttcgataga
     7681 accgataaag aggataggca caactatcac cttattctgc ttgccaagaa caaggagggt
     7741 taccataacc ttatgaaatt ggttagctat ggctataccg agggttttta ctacaaaccc
     7801 cgtatcgata aggaactact tcgcaagtac agcaagggaa ttattgcctc aagcgcttgt
     7861 ttgggaggcg aaatccctca ggccatcctg aatggtaata tcgatgaggc cgaaaggctc
     7921 atttatgagt ataaggagat ttttggcgac gatttttacc ttgagctaat gctgcataag
     7981 tcgggtgagc cccgtattga ttatgaggtt tacgagaacc agctaaaggt aaacgaggtg
     8041 cttattgagc tatcgaaaaa gacgggagtt aagtgtattg caaccaacga tgtgcatttt
     8101 gtgttggccg acgatgcatc agctcacgat atccttattt gcctcaacac cggtaaaaag
     8161 ctaagcgaaa caaaccgaat gcgctacacc tttcaggagt acctgaaaag cgaggaggag
     8221 atgctggaac tttttcccaa ctgtcccgag gccataagca ataccatgga aatttaccac
     8281 aaggttgagg tttacgatat atccagtaaa cctattatgc cttattttcc cttacccggt
     8341 gggtttactg acgataacga ttacctccgc cacctcacct acgaaggggc aaagcaacgt
     8401 tggggcgaga acctaagcga taccattgtt gagcgtctgg agtttgagct gggtgtaatt
     8461 gcccgaatgg gataccccag ctacttcctt atagtttggg actttattaa ggctgctcgc
     8521 gatatggggg tatcggttgg gcccggtagg ggttctgctg ccggttcagc agtggcctac
     8581 tgcttacgca ttaccgatat cgaccccatt aagtacgacc tgctgtttga gcgtttcctt
     8641 aaccccgagc gtgtttcaat gcccgatatc gatatcgact ttgatgaaga cgggcgtgag
     8701 gaagtgttgc agtacgttgt taacaagtat ggcgctaagc gtgtggctca tattgtaacc
     8761 tttggtacaa tggcagccaa gtcgtcaatt aaggatgtgg cacgtaccct tgaacttgaa
     8821 ttaccaactg ccgacaggtt ggccaaaatg gttcctgagc gcccaaatat caccctaaaa
     8881 aaagcatttg aggaggtgcc tgagcttgaa aaggagacca aatccgacaa cccgttaatt
     8941 cgtcaaaccc ttaaacacgc tcaggtttta gagggtacag tgcgccaaac cggagtgcac
     9001 gcctgcggta ttatcattgg ccgcgatgac cttgaggaat acattccgct ctgcacaaac
     9061 tccgatgcaa agctgtttgt tacccagtac gaaggcaccc atgtggagga cataggtttg
     9121 ctaaaaatgg acttcctggg gttgaaaacc ctgtccataa ttaaggatac ccttcagctt
     9181 attgagcaat caacaggcaa aaaggttgat atcgacacca taccgcttga cgattccaaa
     9241 acctttgagc ttttctcgcg aggcgaaact actgccatct tccagttcga gtcgcctggg
     9301 atgaaaaagt acatgcgcgc cctgcagccc aaccgctttg aggatcttat agccatgaac
     9361 gccctttacc gcccgggccc catggattac attccaagct ttgttaaccg aaagcacggc
     9421 cgtgaaccta ttgtttacga catccccgag atggaggagg tgcttaagga tacctatggc
     9481 atcaccgttt accaggagca ggtgatgttg ctttcccaaa aacttgcagg ttttacaaag
     9541 ggtcaggccg acgccttgcg taaggcaatg ggtaagaagc tcaaaaaggt aatggatgag
     9601 cttaaggaga aatttattga gggttgcctt agtaaaggat acgataaaaa gattgttgaa
     9661 aaaatttggt ccgactggga atcgtttgct gagtatgcgt ttaacaagtc gcactcaacc
     9721 tgctacgcat acgttgccta ccaaaccgct tacctaaagg cacacttccc gagccagttc
     9781 atggctgccg tgcttagccg taacctttcc gatatcaaaa agattaccac ttttatggac
     9841 gagtgccgcc gcatgggtat tcaggtactt ggtcccgatg tgaatgagag tcagattaag
     9901 tttgccgtta tgccaaacgg cgatatccgc tttggtttag gcgctattaa agggatgggt
     9961 gagaatgcag cgcaaaacat cattgaaata agagctaaag gagggaagta caagaatatc
    10021 ttcgattttt ttgaacgggt aaacctacaa actgtaaata agaagaatct tgaggttctt
    10081 gcagttgccg gcgcctttga taatcttatt gattttgacc gcagtgtact attggcagtt
    10141 gatgctaagg gtgtatcgta ccttgagcag cttatgcgct acgggattaa ggtacaatcg
    10201 gaaaagaata gtactcagca atcgcttttt gggtcggttc ccggctttga ggttcctaaa
    10261 cctgcaccgc ccaaggcaga cccatggcca accattgaaa agctgaacaa ggaaaaggag
    10321 gtgataggta tttacctttc agcccatccg ctcgatgagt tccgttttga aattgaaagc
    10381 ctttgcaatg tgtccattgc cgaccttaag tccaacatgg aacagtatcg cgacagggat
    10441 gtaaccattg ccggcatgac catagcctcg cgaatcgata cagctaaaaa tgggaaacag
    10501 tatgggcgca taacccttga agattacacc gattccattg atatcatgct ctttggtaag
    10561 gactttgaga gctaccggaa cttttgcttt aacggctact acatcatggt caggggtaag
    10621 gttcagccta aagcctataa gcccgaggaa cttgagctac acatcaagtc aataaacatg
    10681 ctatacgatg tgcgtgaaaa gatgataaag agcattacgc ttaaccttcc cctcgatgag
    10741 attaacgata tttttattga cgatattttg aagtatgtgc aacgcgataa ggctaatatc
    10801 accctaaaat ttaaggttta cgatccggag taccaggttt ctgtggattt attctccagg
    10861 gcttaccgcg tgaacataac tcaggatttt attgattacc ttaacgatag cgagattaac
    10921 tacaaagttg ccatggagta aaccttttat caaatttttg gtttaaattt gtaattgttt
    10981 tgatatcgac aaatctaact taaaacatat aaactatggc attagcagta accgacgcaa
    11041 attttgatga gcttgtgctc aactccggaa aacccgcaat ggttgatttt tgggcagagt
    11101 ggtgtgggcc ctgccgtatg attgctcctt acgttgaaca aatggctgag gagtataagg
    11161 ataaggcagt ggtagcaaag gttgacgttg acagcaaccc aggcattgcc gcacgctatg
    11221 gtattcgcaa tattcctacc gttttattct ttaagaacgg acaaatggtc gataaacaag
    11281 taggcgctgt acccaagaac gctcttgttg ctaagctcga ggcattactg taagtatttc
    11341 aatcaccata aaagccgggt gtgctttacc cggctttagt ttttttaatt tgatagctat
    11401 gcgaatttgt gtttactgtg cttccagctc aaaggtagat gccaaatact ttgaggcgac
    11461 taaagtttta gccaaggacc ttgtggccaa aaatgtaaca attgtttacg gtggcggctc
    11521 ccatgggctt atgggctgca ttgccgatac cgctttagag catggtggta aagtaattgg
    11581 cgttttaccc cgattcatgg agaaggtgga gtgggggcat aggaacctta cccagctcat
    11641 actggttaag gatatgcatg agcgtaaaaa gctgcttatt caggatgttg atgcggttgt
    11701 agccttgcct ggcggttgtg gtacacttga ggaactcatg gaggtcataa cccttaaacg
    11761 gcttggcaag tttaccaaac ctattataat actgaacact gacaatttct actaccacct
    11821 taaggagatg ctccaaaaga tgattgatga acgattcatg cgtgatgagc ataatgccat
    11881 ctggcagttt gttgataatc cggaggaagt aatacctgca atactaaatg ctccccattg
    11941 ggattccgat gcaattaact ttgcggcggt ataaatagtt aaatcaatga ttccgtaata
    12001 aattctcctt ttagataaac ttattgtttt gttgtattaa gcgcacttaa ctattactta
    12061 atgtcgttat tcattaaaac tcgttcctat acacaaacag cctgctatcg ccaatttcat
    12121 cgtacgatac cattttaccc ttgagggtag gggctttaac cccttcgcca aagaacttat
    12181 tgcccacaaa cattcgggct tcgtcccaaa ggtttttctg tataaagctg ttgagtagca
    12241 ttgctccgcc ctcaattatt actgaaagta tttccctttc tgctaattcc ttaagtattt
    12301 gagcctctac gccttttaca aagtcaatgg ttataatctc caggttatcg attcctgaaa
    12361 attcagtttt tcgcgccaaa gcgctactat tgtttccggt aacaagaagg gttggctggc
    12421 ttccatcaaa cacattggag ttaaggggca agcgcagctt gcggtcaaca accacgcgta
    12481 ttggtgaacg accactccag ttccgtacgt taagctgggg attatccttt tccaccgtgt
    12541 tagttccaac aagcacagcc tgttcctcgc tgcgccagcg gtgaacaagg gaacgtgcca
    12601 gctcattggt tatccagttg gagcctacgg gttcgttggg ctcacgtttt ttatcaataa
    12661 aaccatccat ggtttgagcc catttcaaaa ttacgtaggg gcggttcttg gtatgatagg
    12721 taataaaccg ccggtttagc tcaatggcct cgttttcaag tatcccaact gtaaccgctt
    12781 tgccgttttg cctgagtatt tcaattcctc ttccagcaac ctttgggttt gggtcagtgg
    12841 tagcaattac tacccgggga atttcgtatt caacaatcct gtcggtgcag ggtggggttt
    12901 tgccatagtn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ggggaatttc
    13021 gtattcaaca atcctgtcgg tgcagggtgg ggttttgcca tagtgtgagc atggctcaag
    13081 cgaaacgtat agtgttgatt ctttgagtaa ctccttgttc tgaaccgaat taattgcatt
    13141 cacctcagca tgggcttgcc cgtacatggc gtgaaaaccc tctccaatta tttggttatt
    13201 atgaacaata acggcaccaa ccataggatt tggggcaaca ttaccctgcc cacccaaggc
    13261 aagttcaagg catcgggcca tgtattttcg gtgaacatcg gcattctcca taaaaaatgt
    13321 aattttgttt tacacaaagt taagcaattt tatggagcat atcaccctta gcggtttact
    13381 taatcatgtt tccaggagtc ttagtggaag tttcaatgag aacgaactac acaccataaa
    13441 aaagtacata gcagaggcgg tgttacatgt tccataccat caggtgttta tcaacaagca
    13501 tcatgaggtt aagcacgagg atgctcagga ggttgaacga atcatttctg aactcataag
    13561 cggtaagcca ttgcaatacg ccattggttt ggcttacttt tccgacttag ttttaaaggt
    13621 tacgccctcg gttcttattc cgcgccccga aaccgaagag ctggtgtcgt taattgtgaa
    13681 ggagaatagg attgttgccc caagaattct tgatgtagga acaggaagcg gttgtattgc
    13741 ccttgcacta gcaaagcata ttcccgatgc aagcgttgct gcaatcgata tttccaccga
    13801 tgcccttgat gtggcgcggc agaatgcaaa atcaaataat ttgaatgtga atttttttca
    13861 gggtgatatt ttacaaccca cagtgctcga cggttatacc ttcgatatca ttgttagcaa
    13921 tccgccctac attagggatt cggaaaaggt gctaatgaac aggaatgtac tggactttga
    13981 gccgcacatt gctctgtttg tccccgattc cgacccctta aagttctacc gagcaatagc
    14041 cctacactcc gttaggcgtt tggaaactat gggctggctt tggtttgaaa ttaatgaggc
    14101 tcttggttca gagattgtca gccttttaaa atcattagga tttggtaatg ttttgctgat
    14161 tgacgatttt agaggcaaac cccggtttgt taaggcccaa aagtgtaacc catgattaaa
    14221 ggcgaaaacc ataaagcgtt aactccccaa aaggccatgg agcgcttaca atacctttgt
    14281 gcccgacagg agaaatgcac tcacgatttg gtgcaaaagc ttaagcagtg gggttttgct
    14341 caagcagaaa ttgataaaat agttggcaag ctctcatccg atgggtatgt ggacgataac
    14401 cgctttgccc ggctttacgt tagggaaaaa agccgaataa acaaatgggg accaataaag
    14461 cttagagcaa tgcttgtttc aaagggtatt gcaaaggata taatcgatca ggcccttaat
    14521 gagcttagat atgccgaaaa tccggttaat ctttttgacc tccttaacaa gaaaatgccc
    14581 caggtaaagg ctaaaaacag gttcgacttg cgtaataagc taattcggtt tggcgcttcg
    14641 cggggctttg ccctggacga agtgattgat acggttaacc gtttgcttgg agctgaagag
    14701 taaaaacctt attagccctt aatcacctta accgcgccaa gatacttacc attgagatat
    14761 gctgtgagta tgtaaatccc tttctgcaaa tcctgggcct gatatcggta cacaaacgac
    14821 gttagggttg cattttcgct catttgccaa accactgatc ctgaaatgga tgtaagtttt
    14881 agggtgattt gacctacttc gggtagtatt acccttaggt cgaagtattc gttgaacgga
    14941 ttgggtattg ctgagatcat aattggatta ggctcgtcct tgcatacccc gggtaggtta
    15001 gcatcaagcc aaagtaaccg gctggagacg aaatttttca ggtagttgac ctcctgctcg
    15061 tatgtgcttc caacaaatgc attaggccaa acccatacac caagtgagtt gaaggtattg
    15121 tagtttcgta ttctggcatc ttgtattagc gttgtcatgc tatcgataat ggcatggaca
    15181 ccggtacggg ttagtaatcc actactttta agttcttccc atcggcatcg agccatattg
    15241 ttgaacttat cgtcggtggt aaacttatcc caccaaaagg gaggttggta gtaatcgtcg
    15301 gtgctaatcg atttgaaaac ccaaccctgt ggcgagtcgc cactgtaata gttggcattg
    15361 ccaaaggtaa ggtcgtaatc ccacattggg ccaaaggtta acttacctcc cttactatcc
    15421 ttatccttgt gcaggaaagt gcttatgcgg taggcatcaa tgttttttga taattcatta
    15481 accaaaaagt aatcaacagc cgatgccata ttaacgtagc ggtagtagcc caggaaaggg
    15541 tcgttccagt acgatgagtt tagggtattc tcaaaggtgg tgatgtagtt gcggatataa
    15601 ttcttttgaa ccggtaatat attctctggt ttagggtagt gccaaaggaa attaatgggc
    15661 tttccgttgt gtgtataggg cgatgcccaa ccgaactcat caaaatcacc cttatcaatt
    15721 ttaactatgt agccaccagt taaatcatcg cctgaaatat cgttttgggt aagtttagca
    15781 atattcacgc ggtttttatc gcgacgaact ttctcaataa gaaggtatag gccggcatac
    15841 tgttcgttga ggattagctc acagaaagcc gttctggggg catagcgccc catgcgccta
    15901 ccaatttcgt atgcaagagc gttacgaatg agcgatttat cgttgtaagg cccgtaaagc
    15961 gcccagtcgt tgccgggggg cattcccatc agggcagagt caatgttttc gcccagtgcg
    16021 ttaattagct ccacattgta gcttttcttt ggccagtcgt tccatagcga cgattggcca
    16081 cggtactcaa gatgaatagt gccattgtag gtaaaatcgt tatcggctat actgttaggg
    16141 gggctttgct tatcaacaac tttcatggtt gccactatct cgttgtcgga ttgtacggtt
    16201 tgcccatgag tatcaataat cacaatgggc agattactac tgagtgcggt tacgggtggg
    16261 ttaaaccagt cgggtgtgag ctggtagtat gaattactta ccgtaatgcc taccgacagg
    16321 tatggaataa tggttaaatc cgatgagcca ctacttgtat tatgaacctc aatggccaaa
    16381 acgttattgc cattccttaa tgcgcttgca agtatatctt tttttatcag aaaatcggtt
    16441 ggcctaccgc ctgtatacat cactgcctca cggttttccg acgaaggagt atcccagttt
    16501 acatttacgc cctgcaaacc tgccgagcgt gctatttcaa ctccattaat ccatgcaata
    16561 aaggcgtcgt cgtaatccac gcttagcaca gccattgcta tctcattgat atcggaaaca
    16621 ttaaaggtag ttctgatgct taccgatatg cagttgggaa cagttgtggc atcgtcgtta
    16681 tcgccatagc caaaccctcc ctgacctagt gaccactggc tatcgtcaaa gttggataaa
    16741 ttccaacctg taggcgttgc ggtttcattt accctatatc gccaattgtc gcttggataa
    16801 atggcagtct cccaatggtt tatgctttgg gagaaggcac tctgtacaaa aaggaggaaa
    16861 acaagaaggt tagctcgcat ttaacaaaaa cttaaccttc aaaaatacta aaaggattta
    16921 gcgaaatgct acgatgaaag aaaagtagta gtatttatat cgattttaga tataaattta
    16981 ttcttctgcc aaaagcgtaa tactgcttaa attgccttgg taatatcttt cgaattgcca
    17041 ttaaagccat gtgtaaataa aaaatgaagg ctaccgtgaa ggcagccttt catgctataa
    17101 gtttttaacc cttttcaaac taagcagatt agtaatccag ttcggtatgg gcttaaaggg
    17161 atcgcgctta tccatgttaa gttggatgcc aaggggtttg atgagtggca gccgatgggt
    17221 ttcaacaaac tcaatgagag tactatcggg atcttcgatg tacgaaaagt gccctgcggc
    17281 ttcacccata tcaaaactac ctttatcgtt gtgttttacg tttgaattca cggtgaaagg
    17341 gaatcctttt tcaacgcact ccttttcaag gtcgttcatg tttttcatgt cgaagcatag
    17401 gtgaataaat cccaagtcgc cccagaaacg gtttttgaaa atcttgttgg gttcacggtc
    17461 gagtgcttgc accagttcaa tttccgatat gccgaagaag ttgctgaaac tacccttcct
    17521 ggtatcggag tgtgtgagca gcatacggcg gaaagtccct ttgccaccgg gtaactcagt
    17581 aaggtcgtca aaggttcctg ttttgtcgta aatagctgtg tcgtatccca atatatcgct
    17641 ataaaccttt aaagctttat cgatattgct aacgcctata atggcaccgc caatacccga
    17701 tacaggcttt ttctcgtttt tgaaaatgta gctgtggctg tatatctcga aaaagttacc
    17761 aaatgggtct ttgataaaaa aatgctcttc gccattagga cgaatagtaa gttgacttac
    17821 tggaatgccc ttggtttcat agtaattaaa ggctttgtga atatcggggc atttgatttt
    17881 agctgcaaaa attccatgat cgccaagttg aacctgaaac tcaggcatta gcggagtgcg
    17941 acctttgtac tgccaaattt caaggcctcc gccgccttgc atgttaagag caagggcagc
    18001 atggcgctgt tgtggttgcc ctccggtata ggggagcatt agctcggcaa ctgtattgtc
    18061 ttcaaaaata cgaatgtcaa atccaagatg ctccttgtac catttccatg cctccgttag
    18121 gtttgatact ccgatgccaa cttgctgaat accgtatatt attttcgaca taatgtttat
    18181 gtgggtttga ttttggttac aattctgaaa aagatgcttg ggaataggcg tttaatgtgc
    18241 accatcaaaa tctcactttt gcctatgtaa acctccggtt ttcgtttttt aatggctcga
    18301 atgtactgcc gggcgcattc ctttgcactt agtccgttca gctgaccagg gtccattacg
    18361 ccatgttcct tacccttttc gtcgatggct ctgagagaga tattggtttg gatgcggccc
    18421 ggataggcaa tggttaccga tatgttgtgg ggtttcattt cggcacgaag tgtttcaaaa
    18481 aaaccctgaa tggcatgttt tgctgctgcg tacgatgagc gtaatggaaa cccaaacttt
    18541 cctgatatac tacttgtagc cgcaataaaa ccttcaccgg tttcaatcat accgggtaaa
    18601 accgctttgg taattataat gtaggagaag taatcaatct ccataatttt ccggtcgata
    18661 tcaactggtg tatccttaac aagagaacgt tggctaatgc caccgttgtt aatgagcagg
    18721 tagatttgtt cgtaagtatt aatgatggcc tcggctgtgg ttttgacctc ttcgggattc
    18781 gaaaggtcga aagggtattc ctggcagaat gaggtgaact cgcggcactc atcggccacg
    18841 tggcttaact cctgggcatc gttcgacgag ataatcagat gagccccttc ctcagcgagc
    18901 tgtaaagcca attcctttcc tatgcccgat gaggcaccag ttatccaaac aagtcggttt
    18961 ttaaatgaaa tcatcaaaca tcaaattata ctttgagcat gtgtatgccc aaaaattttg
    19021 atgtcaaatg taacactttt ttataaagtt aattctgaga acctagtatt ctttccgtga
    19081 ggtttccttc ccgttttcgt cataaatgat ccaggtaccg cttttttcgc catcggtata
    19141 ggtcatatcg tatcggagag tgccgttatc gtcccaaaca taccatttcc catgtttttt
    19201 acccttcaca aagtttgctt cggaaacctt tatgcccatt tcgttccatt tctcccatcg
    19261 gccattcttt tcaccaaggc tgtaggtcct aatttcctga atttttccgg ttgggtagta
    19321 tagtttcatt tcgccatcgg gtagtccgtt ggtgattaag gcttcaactt taatgtttcc
    19381 attgtcataa taggaggtgt agcgacctga atacaatttc ccattactat cataatattt
    19441 accctcgcgt tcaacaacat tggtttgggc aaagctaacc agcgaaacta atgagatgag
    19501 taacgttaaa aataatttca taatgtgcat tttagactta ttttacgata attacttcaa
    19561 aaaaggtgca aaaaaacttc ccgaatatcg ggaagtttat tgattttatg aaataaaagc
    19621 actagtaatt ggtattaaca ggatcccagt tggtccatcc ctgagcccag ttttgggttc
    19681 caaaagcacc gcggaaagta actgtctcaa atcctgtgag tccggtaaac gaagcaccag
    19741 ttaatgcagg ggaaccgctt tgtggcatgg cgtttacatt accccagttc caggcattcg
    19801 taagttgaag ttgggtgttt tcagcataaa gggtattgtt acctgcatta aacatggttt
    19861 cgtattgcgc ctgtgttaag cttgttccat cggttttagc attaaagttc tttaccatac
    19921 cagccattaa acaattcttg atttcgggac cactggcagc attgtaaagt tccatcgatg
    19981 gtccttctag agaaattcca gtattaaaac cagctataat ggagttgtat aggttcaaac
    20041 gggtatttcg gcgtaacctt aagccgaatt tatggttggg gtttacgttg gcttgagttt
    20101 tagtggcata agggccaaga attgtaatgt tgctaaactt tgctgaagta agagggctat
    20161 ttgatgaacc gttagcatca ttatccgatt caaaaccgtt ggaagcatcg ctcttatcgg
    20221 cttcttccgg accacgaagg attaaaccaa actgcacatt accgctgaaa ccattatcgg
    20281 tatcaaagtc gtcgtcgcca ccgcgatagg aaatcaggta tttagtattt actgttccgc
    20341 caaaccattc gaatgaatca tcgcggccga atgaaacctg tacatactca atggtagttc
    20401 ctcggcctac gccgcccatg gttaaaccat taatttcgtt accattggaa acttcatagc
    20461 cagcaaactc aattcgcaca taccttagta tgcctgagtt atcgttaggg tctgttccac
    20521 cgtatacagc accggttcca ccttcaattt ctgcttcacc cccggtttgg ttaatagggg
    20581 ctttaccgca aataattatc cctgcccagt cgccgggttt acgttctcct actggcttat
    20641 cgctggtgaa aataataggt ttttcagcag ttccgtttgc tacaattcta gctccacgtt
    20701 ccacaataag gcatgccttt gagtcgctta acccttttat cagtgtacct ggttcaatgg
    20761 ttaaggttgc tcctgattta acatacacat agccatcgag aataatggtg tcctttgctt
    20821 cccaaagggt gttttgtgaa atttcacccg atacggtttt tttggttcct ttgggggtat
    20881 cgtcgtcgtt cttatcgcac gatgttacca ttaagcctaa tagggccact gcaattaata
    20941 acaaattact ttttttccga ttcatacaat tagtatttag aggttagttt atttcttttt
    21001 cacaatccaa aagtatccag caaatcgaac tttaacgtta aggtgagctt agctttacat
    21061 tagcagtttg ttacattaag gttaaggtga aaaaaaagag ccggattagg gcatccgact
    21121 ctggtttagt atgttatgtt aacttctaaa ttctactcaa ctgtgtttaa ctcaatcttt
    21181 acctcctttg aacttttttt cacctcaacc tcagcaggtg catacgatat caaactaaca
    21241 cataattttg ccttaggctg agtaaggttt attgtaaagt taccctcaaa atcggtataa
    21301 acaactgtat tctcatcaag cttaaccgct acaccggcca gagcctcgcc tgttgtttta
    21361 tcaactacct ttccactaat ggtgctaacc atgttggaag tagcttcagc cgattccttt
    21421 ttagcatctt cgttagcagc tataaggttt agtgccaaca tgctaactat taaaactgac
    21481 agtatttttt tcattgcttt atggttttct catttaacta ctgcaaagta aatactccaa
    21541 tattgcctca ttattaccca aaaatgattt gtatgttaag tttatgtaaa gtgtataaat
    21601 catgctagga aaaagaaaag ccggatgtta aaactaatct aaacatccgg cattggtggt
    21661 taaaacctac actaaaacct aagattggca ccaagggaaa atgttatacc ctggttgtat
    21721 aatttattgt tcatagtcat atcgttgttg tttgaaccta tgaaaggctg gtatgtgtta
    21781 tatttttggt ttaaaagatt ttttacgcca gctttaattt caaccttttt accaaactct
    21841 ttcgatacag ttatgtcaac taaatgttgg tgcttttcgt agtaatcggg aatatcctgg
    21901 tcctggttct ggaaaggaat tccaaccgcc agaattctat cccctataac attgtactgc
    21961 acggaaacgc ttaggttgtt tttatcgttc tggtagaaaa taccagcatt aaccacgtat
    22021 ggagattggc cctgcatggg tctattcctg aaaatacttc cttcagggaa aataacctta
    22081 ctatgtatat acgacgcatt caagaccatg taaaaatctt taagcaatgg aatggcttgc
    22141 agggacttgc gaaggtcaac ttctacacca aagctatagg ccccttcagc attattgtaa
    22201 gtgaagaggg gtcgagcacc agctggttta aaaacaagtt caattgggcc attaaacttt
    22261 ttataaaaaa gcccaattga aaaggtttca ccagtattgg gataaagttc ccagcgtaag
    22321 tcgtagttat gaatgaggca gttcttaagg tttgtgttcc ccacaaattc agcttcatcg
    22381 gtaaagttat agaagtagaa tggggcaatc tcgcggtatt caggacggtt tatagagata
    22441 cctgctgcca gcctgataag gtttttttcg ttaaagttat aggtaatatt tgctgatggg
    22501 aaaatatcaa tgccatcatc ctcaacttta actggcctac cgtaacggtc gtaactgttc
    22561 agaatctggt tcatccattc cgccctaagt cccccataaa atgataattt tttggtaata
    22621 tttgtgttaa gggctatgta tgctgatccc aaaatggatg aaacgttgta tgagtcagat
    22681 tttgaggttg actctctgag caaaaaccca tcggggcttg tgttgatgtt ttccttacta
    22741 aaaatctgat cgataggtaa ccaaaccgat tcagtgtacg atgaattttt ggcaaatccc
    22801 agaatgcgtg cgtcaaagtt gcgtgcctta aattccccgt aagtaccgct ttttagggta
    22861 ggttcccaat tccctatttt taattttctt tcgtagttga ctcctaaact ggcaatatgc
    22921 tcaacaagct tcatgtaaac tctacccgca tcgcttactg agggggtaat tccaactgct
    22981 gcgtaatact cattgtagtg tggaataagg ggttcatcct gcagggtagt gcgtagctgc
    23041 ttcaggtcag gctcgttcct gtatgaaaga gcatagcctg taacccaatc taatttattc
    23101 ttttgattgt cgccccagcg gtgttctcca ccaagctgtg atgagtaggt tgttcggttt
    23161 tgaaagttat cctggtagga acgaatggta aaaccttcat gcccatttag cccatttcgg
    23221 attattgcat tgcttttacc aattaggtta aaaaggttac gaaattcaat tttactccct
    23281 ttaccagtga aaaacgacca gttgtgaagc aagccaattt ttacttttcg ggtaaatata
    23341 ctatcggtat agttatggtt aaagggaggt tcctggccag gcactaactg cacctggtat
    23401 tcactatgat taacttcttc agtctcaaag gtgttgctat aggaaagtga agtaatgtta
    23461 cccaagcgtt tattcccttt ctgccatttt cttccaaagg aaaggttgaa cttttggtca
    23521 ggtactgctt gtacactttg gggcgcccaa ctggtgttta atttctgccc taatcctgct
    23581 agctgtgagt ttgttaagcc tttaagtgtt gatggaaagt cggcgggtaa attccttgta
    23641 ccgtcatcaa agcccagcca atcggtttta cttttggtta tctgcttaaa attgccatag
    23701 tttgtgctag tgttgaaccc agtagcataa ccaatgctaa gaaagttttc atcgggcata
    23761 tttttcgtag agattttaat aaatccaccc gtaaagtccg ctggtagctc tggcgagggg
    23821 cttttgtaaa tcaccatgtt gtcaatcatg gcacttggaa taacatcgaa ggaaaaagct
    23881 ttttgatcgg cttcgctgga aggagttgaa gcattgttaa tccaggcgtt gttataacgc
    23941 tggtttaacc ctcgcaccac aacaaacctg tcgtcaataa ttgaaactcc gggaacacgt
    24001 cgtataacct ccgaagcgtc gcgatcctga gaacgggtaa tttgctgtgc cgatattccg
    24061 cttgccacag taagacttgc ctttatggtg cttaacatgg caacctcagt gtttgtttta
    24121 cgtgcggctg ttacagtaac cccttcaatg gcagtggtta tttcttcaag ctcaactttt
    24181 actactgtac ttttattagg ctcaacttta atgttattca aaattacggg ttggtaggag
    24241 ataaaagaaa ccagaacatt gtaagttccc ggattaagat ttgataaggt aaacctacca
    24301 tcaaaatcgg taatagtacc ggtagtggta ccctcaatta ttacattggc acccacaagg
    24361 gtctcgttgt gcgacctgtc gtaaaccaca ccttcaattg atcctttttg tgcccaggcc
    24421 tcaaaaacaa caaaaaaacc tgaaagcaaa agcaatacaa tcttattatt agttttcacc
    24481 atagtttgtt tagtatttcc tactttttta cagctacaaa gtaagccaca ttaaatggca
    24541 tagattttaa ttccacgtta ccgttttgtt aatctaaccc taacccgttt tgtaaaaaag
    24601 ttaactttga gttaacttta aacacatgct aagcctttta tatttgtaaa aacaaaaatt
    24661 taaaggaatg aatgtatcac ggtatagaat cctgatagtt gatgatgaac ccgatattgt
    24721 agagttcctc agctataacc ttaaaaagga ggggtttcaa attcaaactg cctccaatgg
    24781 ccgtgaggcc atcgaaaggg ctatcgattt ccgaccacac ctaatactcc tcgacgtaat
    24841 gatgcctgac atggatggaa ttgaaacctg tgaacagctt cgtaaacagc catcaacttc
    24901 aaatacttta atagccttcc ttacagcgcg cagcgaggac tacagccaaa ttgcaggatt
    24961 cgatgctggg ggcgacgatt acatcaccaa gccaattaag ccaaaagtgc tgataagcag
    25021 aataaaggca ttactcaaac gaagcggcac cctcattgat gatgctgaaa gccctgatac
    25081 tataccagcc gaaggaattt atatagataa ggaacgctac tccgtttatg taaatgggaa
    25141 tgagctatcg ctcccaaaaa aggagtttga gctacttgct ttgctttaca gcaaacccca
    25201 aaaggttttc acccgcgatg aaatttacca agccgtttgg ggcgatgaca ttgtggttgg
    25261 cgatagaact attgatgttc atatccgcaa gctacgcgaa aaacttggtg atgactacat
    25321 taaaactgta aagggggttg gttacaagtt tacttcgtaa atcaagttga tgttgtatat
    25381 tatttgcccc caatttgtaa aattacccta aacccaattg gagtaatgtt tgtaaagata
    25441 tatttttgaa aaaataattt ttaatttgat agctcaattt ttgttaccat ttatatgcaa
    25501 atagcaattt ggaaacccga gtatagtgtt ggtgtaaaaa gtattgatga ggatcaccaa
    25561 aaactcttcg atttacttaa ccagcttttt gagtcaatga ctaagggtaa agggaaagaa
    25621 ataataaatg atattatttc agagctggaa aggtatgcgg ttttccattt tgggagagag
    25681 gagtcgtatt ttagggtaac taactaccca tttgctcttc agcatattaa ggagcatcag
    25741 tacttcaagc agaaagtagc agagcttaaa aaggatgttg cattaaacaa aggcatggtt
    25801 gctccagatg tacttggttt cctgagcgat tggttaaaga atcacattgc caaaagcgat
    25861 aaggcctacg aagatcattt aaagaaacac gctgtaattt aataaataca atttatttcc
    25921 attttaaaat ggttgacaca ggttttatgg tcttgaatta attttagttc aatccataaa
    25981 acctgtgtca tttctattat aatgatattg tttcttagct ttataaaatg ggctgctact
    26041 caatattgtt tagaataaag tcccgttgag ctttcaggat gttaattgta tgtaaaagca
    26101 ggttcttaat ttcgtttagc aatccaagat aaagcacgct attgcgagtt cctgtttcac
    26161 cctgtttaat acgttttacc tgtttcttgc gtattttgtt cagatcttca aggatatcct
    26221 gctgcttttt gatagcatcg ggaacgcggg tgtaatcgtt ctgcttaatc atttccagaa
    26281 tatcatcgaa gagatccgaa agctggttgc tcaaatcctt cagttcagca gcctgctcac
    26341 gggttaaccc cttatgttgg ttctcaacat gctgtaagct ggggcgtgag acaaactcca
    26401 gggagtgggc aagctcacgc aggtaatcaa ttacctgaac gtaataatga ccagtttcaa
    26461 ttgagtcggc ttcaagctgc ttgagaacat agtatacgtt atactttaac tttttggctt
    26521 ccatgttaag ggcctccacc tcctcgttta cctccttaag ctgccggcgg ttctcggttg
    26581 ttagcccatc aatggttcta ttgtaaacct ttattgactg ttttaagtat ttacgtaatt
    26641 cattggtgca tcggtttacg atactaactt catcaactat ctcctcgtca gttaattgct
    26701 tggtcttttt cttgtgcaac aggtgtgatt taattagtag accaatagct atagccagta
    26761 aaataattag tgcgggtgct ttgccaaagt aaagtgcggt agctattcca aaagcaattg
    26821 taaaggttac aaaggcagta aagaaccatc ctgcaataac tgtaattact cctgtaatac
    26881 gataaactgc gctttcgcga ccccaggctt tatcgctcag cgatgttccc atagcaacca
    26941 taaaggttac gtaggtggtt gaaaggggga gctttagtgc ggtagccgaa ctgattaata
    27001 tacttgccat tacaaggtta acggaggctc ttactaaatc gaacgatggg gcatcctttg
    27061 ggttagagta tcggggtgct ttagtggtat cgaactgctt agcaacaagt ttcctgataa
    27121 aacggggtgt aactttatcg acatgctctg caaaccttcg ggctattcga acaattatcc
    27181 ttgaaaactg tgtacttcca aaccgctcca tacctgcatc ctgacgggct aagcccactt
    27241 cggtatcggt aactgttctt gctttgcgcg atgtccaaag ggtaattacc attattaagc
    27301 ctgcaatgaa taggtacata aagttagtgc ttacctcacc agctaattca gtcattctca
    27361 gcgattcagg agcgatatcg ggatttgctg catgtatttg gtaagctttt agtccagcca
    27421 tcgaaacacc aataaagttc acaaggtcgt tcccggcaaa cgataatgct agggcaaaag
    27481 tacccataag tactataaac cttagtacat tcattttgaa caggctaata agtagttgga
    27541 atactactgt ccaaactaaa aagctgtaaa gtattatgct aaatgaatga tctgatatat
    27601 attatggttt ccttggaaat taaggtagta tcttttacgc ctttcattac tataaaatag
    27661 gtgatggctg tcatggcaat accaccccat attccaccaa caaatttcag gtttttctgg
    27721 tagttaaagc tgaaaagtag tcgtgcaatg tactgaacaa ccattccgac tacaaaggca
    27781 acaaccactg agctcaatat ccccgatata attgctaggg ctttaccact atttatgtaa
    27841 tttcccaggt ttgaaatggt ttcaccctcg gtattcatta tcttgaaaat ggaaacagca
    27901 actgccgaac ctaaaagctc aaacacaagg gaaaccgtgg ttgatgtggg tagtccaaag
    27961 gtattaaaga agtcgagaac aataacatcg gtaatcatta cggcaaggaa aataaccatc
    28021 atctctgtaa agtagaagtt ttccggccgg aatacgccct ttcgggcaac ttccatcata
    28081 ccgcttgaaa acattgaacc
//
LOCUS       DAOSAL010000028        27903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_1251_length_27903_cov_48.049662, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000028 DAOSAL010000000
VERSION     DAOSAL010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 27903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27903
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1251_length_27903_cov_48.049662"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /gene="mnmE"
                     /locus_tag="PK049_05665"
     CDS             complement(<1..927)
                     /gene="mnmE"
                     /locus_tag="PK049_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOK61078.1"
                     /translation="MTLDTTQTIVAIATPPGMGAIAVLRLSGERSLEITSSVFRPAKK
                     GKSLKEQKAYTIHYGSIVDGDREVDHVLVSLFRAPHSYTGEDVIEISCHGSTVIQQQL
                     LELLVRHGARLAQPGEFTLRAFMNGKIDLSQAEAIADLIASTSETARRVALNQMRGGI
                     SNKLTELRNELLRFTALVELELDFSEEDVEFANRQELETLVNNIISEIDKLSASFSQG
                     NAIKAGIPVTIAGKPNVGKSTLLNRLLNEEKAIVSEIAGTTRDAIEDTIHLGGINFRF
                     IDTAGLRQTTDTIESIGIERAFAKIEKARIVLY"
     gene            complement(1606..1869)
                     /locus_tag="PK049_05670"
     CDS             complement(1606..1869)
                     /locus_tag="PK049_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61079.1"
                     /translation="MGTPWVELPRDTIRHAYGVTHVVSLTKPTATSTPAPLPGQAFMP
                     LNASSASFLFREKVGVRSYLTNNLDDPSKSPLRGKTLAMQKVS"
     gene            2326..3741
                     /locus_tag="PK049_05675"
     CDS             2326..3741
                     /locus_tag="PK049_05675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HOK61080.1"
                     /translation="MNTFLESFDYVIQLYNEYVGGYLILALLVPTGIYYSIRLKFLQV
                     SHFKHAIDLVRGKYSKHDDVGDVSHYKALTTALSATVGTGNIVGVTLAIYFGGPGAVF
                     WLWVTGFFGMMLKMVECTLAQKFRKINADGTVSGGPMYYIEHGLKDKLGVWAKRLAVF
                     FAFAGILCSLGTGNMAQANSISDVLKTSYNIPVLYTGIAISALVFLIVVGGLRRIANV
                     TSKLVPLMAVIYFGSAILVIGVHYADIPSALWLIVKSAFTGQAAVGGFVGSTFFMTMI
                     WGVRRGLFSNEAGQGSAPMAHAAAKTPYPMREGFVASLEPFIDTIVICTLTALVIVLT
                     GVWRLDGAVKGVGMTVAAFQEGLGKIGIGFLARHMVAISLLLFAFSTIISWSYYGTRT
                     AQYIFGDKAIKPYYYVFTLFVFFGSVWGIDLVWHFVDMVITFMSIPNLIALLLLFPVM
                     KAEAEGYLKRLKAGEFNLNRD"
     gene            complement(4111..5154)
                     /locus_tag="PK049_05680"
     CDS             complement(4111..5154)
                     /locus_tag="PK049_05680"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HOK61081.1"
                     /translation="MDIHTPIVINGINIKNRVALPPIVNFGWSDSDGKVSSKHIAHYE
                     RIARGGTGLIIVEATCVNPTGRIFSYQLGIWDDNHIEGLARIAEAVHKHDAKVLIQLH
                     HAGLTTRKAVAEKAFGPSVDPENDRSVALTKEQVSELVENFVSAAKRAHLAGFDGVEL
                     HGAHGYLLNQFASSAINHRTDEYGGNSENRLRFARQIIEGIKEVTPSTFIIGYRMAGC
                     SPTLNDGIEIAKLLQAYGVHYLHVSHGGEKGINPTVPSNFDYNHVVYMGTEVKKFVDV
                     PVMVVNQIKSPERANKLVTHGLADMVAIGRDMLTDPDWVTKAKTGEAINLCIDCQPRC
                     KRYTKPESCPLAE"
     gene            5305..5931
                     /locus_tag="PK049_05685"
     CDS             5305..5931
                     /locus_tag="PK049_05685"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOK61082.1"
                     /translation="MTPKKIEKDTESLILEAAKRVFVQKGLDGARMQEIADEAGINKA
                     LLHYYFRSKEKLFMTIFKVELNNFFPRLIPVLLSMEMTYEQKIRHFVEEYIELFLRNP
                     FLPAFVLREANRNPEMIAQFITESGINAEKLKDVMKVLSHELGLSVNEVLHLMVTTVS
                     SCFFPFAGKPIIEQVLFNGNEGDYQKFLSERKRYVADFMIYFLKGRLK"
     gene            5940..7220
                     /locus_tag="PK049_05690"
     CDS             5940..7220
                     /locus_tag="PK049_05690"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOK61083.1"
                     /translation="MRKARIISLTITLLASTYMLKSQNTVTLPGLLKSMEQWNKLYRV
                     SAATDSVYWLRKSNIKVNYLPKVELNAEATWQSDVTKVNIPMSGISIPTPDNDNYKFT
                     IDVSQLIWDGGATKSMHQLEDESRKLEQNKVESELYTQKERVASLYFGILSTDMAIRQ
                     LKAMAVELDKRIEELEAGVNVGVVLESSANALKAERLRLEQSIDANLSQRYSLTESIY
                     SLTGVKIDESAQGIIPSLAIPNQDVCSRPDYKLFDLQNGYLSTAAGALTSRRMPKLIA
                     FARVGYGKPGLNMLSNEFDSFALVGARLSWSIWDWNSTSRERQTLKIQQNILQYRRNA
                     YDEGCSAQVSSALAQIQSLQRQIQTDEKIVELLEKSVKASASQLKNGTITSSVYLSDF
                     NSLLRSRIDMDLHKIKLSQEVVKLYFTLGMSIND"
     gene            7233..8126
                     /locus_tag="PK049_05695"
     CDS             7233..8126
                     /locus_tag="PK049_05695"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOK61084.1"
                     /translation="MNWKNLINVILPTAIVISCSRDNGKADAYGNIESNDVTISAEVG
                     GKLLELKIDEGYKVEKNQLIGIVDTLPQVLKLKQLNAQLKAAKAKLQGIDAQIAVQDE
                     QLNVLKKELDRVKKLVADSAATLRQLDDIEGKFKIAEKQKATFDAQRSGVLAEIDAVD
                     AQIAQAQDLLWRCHMVSPVSGTVISRYVSQGELVITGKPVCKIGTLDTVYARVYIDET
                     QLPDFSIGRKVTILTDTPDGKLKEDKGEIAWVSSEAEFTPKIIQTRNERVNLVYAVKV
                     RVPNPNGYFKIGMPVEVRIED"
     gene            8258..9178
                     /locus_tag="PK049_05700"
     CDS             8258..9178
                     /locus_tag="PK049_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK61085.1"
                     /translation="MPAIQVTNISKSYGKVKALTDVSLSVEEGEMFGLIGPDGAGKST
                     LIRIITTLLVPDSGNVTVFGLDTVSQYKLIRGEIGYMPAVFSLYSDLTVEENLNFFAG
                     VFGTTVKENYELVKGVYHMLEPFRNRPAGKLSGGMKQKLALSCALIHRPRVLILDEPT
                     TGVDAVSRKELWDLLHSIKKQGITVFVSTAYMDEAKQCDRVALIQKGRILGTNTPEGF
                     AEMFPYKLFSIQANNAWSSLDAIRHLSTCETAYMFGDSIHFATNYNEESPESLHDRLM
                     SVGVEANVAQIQASIEDYFIYQLSEKNLVK"
     gene            9175..9915
                     /locus_tag="PK049_05705"
     CDS             9175..9915
                     /locus_tag="PK049_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008148381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK61086.1"
                     /translation="MTNAIEVENLVKRFGSFIAVNHLTFSVGKGEIFGLLGANGAGKT
                     TTIRMLCGLLSPTSGKISVAGYDAYTQPEQIRASIGYMSQKFSLYSDLTVWENIRFFG
                     GIYGLWGKELRIRALELVEEFKMHDFKDTKVESLPVGWRQRISLAVSLVHKPPIIFLD
                     EPTSGVDPVGRRMFWETIHQISSMGTTVLVTTHYMDEAEYCHTVLVMVDGRIEAQGAP
                     AQLKSTHGVESMNDLFLLLSRNGKLNIE"
     gene            9918..11030
                     /locus_tag="PK049_05710"
     CDS             9918..11030
                     /locus_tag="PK049_05710"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOK61087.1"
                     /translation="MAKGRFSNFITKEFKHIFRDTRTLLIVLVMPVLQLLIFGFVISS
                     EIKNANYAVLDYTNDYNTKKLVQRISSSGYFNFKGYLKSENEINEVFKNGDAKLVLII
                     GNNFTQKLQNENFANYQVVTDASDPNTASILASYAQGIVNSFTAEYFGSSQQKERVNV
                     QSRMLYNEEMNSAFMFVPGTMVLILMLVTAMMTSISITREKEMGTMEILLVSPLKPWQ
                     IVVGKVIPYIGIAFTNVLVIILMGYTVFHLPLRGNFLLLMGVSSLFIVLALSLGILIS
                     TLSNSQAVAMFMSMFALMLPTILLSGFIFPIENMPKILQWLSLIMPPRWFLSALKTIM
                     IKGQGLQYVISEVLVMLTMTTVFIALSIKKFKVRLE"
     gene            11042..12145
                     /locus_tag="PK049_05715"
     CDS             11042..12145
                     /locus_tag="PK049_05715"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOK61088.1"
                     /translation="MRRILILLQKEFIQIFRNKTILPIIFVLPIVQLLILANAATQEM
                     KNIRLTIVDFDNSSESRRLINAFDQSKFYTLIPVSGNVDRAVELIDLGKTDAALVIPK
                     RFGESLVEGAPVNIQLMLNAINITAAALTQSYTTGIIQNFASSISIQNINHIKPKQSV
                     RYWYNPELNYKLYMVPGILVILVTIVGMFLTAINIVREKELGTIEQVNVTPIKPYQFV
                     IGKLLPFWIIALFELSFGLLIARLVFNLTIAGSIPLLFAFAAIYLLVALGLGLILSTS
                     ASNQQQVMFMAFFFMIVFLMLSGLFTPTESMPKWANWFNTINPLYYFMKVNRMLILKG
                     SDFTNLKDSFLSISLMAVAVLSIAILRYRKKSA"
     gene            12225..13214
                     /locus_tag="PK049_05720"
     CDS             12225..13214
                     /locus_tag="PK049_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61089.1"
                     /translation="MVLLLFSNLADGQPVFADTSLLDVKLEFGIQQVFADIGEDPSYH
                     KGYLSYENENGATISIPVEIRTRGIFRRKASNCSQPPLLIKFKPKETSNTIFEGIEKL
                     KLVVPCQKSSKYEDLVLKEYLVYKLYQIISPYSYRVRLLRLNMVDSYYGDQSICYAFV
                     IEPVEILSRRLRGVVRDAKNTHPNACNNYYYNRMAIFQYMIGHTDWSIKALHNITLIE
                     PEPFAPSIPVPFDFDFSGFVDAPYALPAEHLPIKSVKERHFNGYCKPEQQFADAFSYF
                     LNLKDTINHTITTFKYLPQAQRNELIRYTNEFFDIIASDTKRKSRIITKCRTD"
     gene            13313..15013
                     /locus_tag="PK049_05725"
     CDS             13313..15013
                     /locus_tag="PK049_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61090.1"
                     /translation="MKSIISKAFFTISVIILLCLSSIAQNGLQVSARLIVKKGDLQGS
                     KVTVTQNGNVVQTISVSNKGVFDFILEYNSEYIIVFEKPGYITKKVLIDTRVPETFRK
                     DIQQMTGFDVVLEKQSEDVIKEYANPVAKIMYARDIRDFTYDTDYSLKVLKEIENENK
                     KLAEEKKKKEEEEKARKAEEEKQRILAEAQKRKEEEEAKRKAAEEARKKEEERLRKEA
                     EERRIAEEKKRLEEQKRLEELKKKAEEEARLKAEEEARKKEELRKQEEERLRKEQERI
                     RQEEEAKRKAAEEARLKEEERLRKEAEERRLAEEKAKLEEQKRLEEQRKKAEEEARLK
                     AEEEARKKEELRKQEEERLKQEQERVRQEEEAKRKAAEEARLKEEERIRKEAEAQRLA
                     EEAKRKAEEEAKLQEAERLKKEAEERAIAEAKLREERLKAEQEKARQEAERLKREEEE
                     RRRAEEQKKLLLEEQRKRDAELRLKLVAEKMSAQIGKEDADQVVTLLAAEDIAKRYTR
                     ERTDEVIRYPRIILTRTVFNRNNTITFYTRVDHEYGAVYYLKDGFFISKEQYLAETQK
                     "
     gene            15020..17068
                     /locus_tag="PK049_05730"
     CDS             15020..17068
                     /locus_tag="PK049_05730"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOK61091.1"
                     /translation="MKEFITACPRNCYSTCSLVVQVDKGKVVGINHLPLNKATPFGGC
                     LKGLSYVERANSPDRIIYPLKKVNGSFKRISWNEALETIAEKLIHFRDVYGQQSVLFY
                     AASGMAGLLNDFSGKFWRYVYGGATTVYGNLCWPAGLEATRLTLGENKHNVPWDLENA
                     KLIVLWGKNPAESNVHEMIPLNNALDKGAELVVIDPRRTQSTQRATLLLQPYPSTDAP
                     IALAVANIIIQNGWIDSDFIKLHVHGFDQFAEHVKPFTAQWASNISGVPVSLIEKLAW
                     YIGNVKPMTLIPGYGMQRYTNGGQTVRAILALSVITGNIGKSGACWHYANLQSYVFDD
                     VLEPLCYYPPDKPDGVIRRSISTARLGEDMLATKNPELKMIWVERGNPLTQNPNTGKV
                     LEAFRKLDFRVVVEQFMTDTALEADIILPAKNMFEQSDIVGSYWNPYIQLKQKVVEPA
                     GEVKPETEIYWWLAQKLGFNEAELSNILLPPGDEHVDRYLDSKLREFNLSLEELRKGP
                     ILAPGLQEIAFSDYRFPTPSGKIELYSEQVQTLWGENPLPTYKPAVELFQNQTPYPLF
                     LMSPNTKNRIHSQFNNLKVISMISGAPVLQINPADAIQRGIGDGDLVEIFNSRGSFRV
                     KAQLTYEVRVGCVVIPNGWWRTQGAPVNVLSEGRETDMGHGSAFHNNRVEVKLVKRKP
                     "
     gene            17065..18366
                     /locus_tag="PK049_05735"
     CDS             17065..18366
                     /locus_tag="PK049_05735"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF016838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HOK61092.1"
                     /translation="MSDRGFIFNYSRCVGCHACIVACYNQNHTEPPMAWRMVVNGNPL
                     KIPLKGFINLSLACNHCIDAPCMTNCPAIAYSRDDETGAIIHSPLKCIGCKYCTWACP
                     YEAPKYNPGKGVVEKCNFCNDLLKVGGIPACAAACPTGALTFGEIKVEANLSKPGFPE
                     VATSPRINVANESINDSLPEMAIEATGLQQSDFAETYSPRIHPTKEIPLFVFTSLSAV
                     LVGWFITFYRLEKISYFSKISFILLLAFAGFASLFHLGKPLRAIRALLHVKSSWLSRE
                     IALFGLFAFSGLLYVLTEKPPLFWFSVVVGTVFLISLEMVYHVVRKNYSTPIHSANTL
                     LTASTLFSLITLSKAFVLLATIKLLLYLVRHAYIQKFNPKKVIFSFIRFLGILIPLVG
                     LLFNLIPANIAPFLTLFLIGEFIDRYEYYASIDTHNPFQSI"
     gene            18507..18896
                     /locus_tag="PK049_05740"
     CDS             18507..18896
                     /locus_tag="PK049_05740"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOK61093.1"
                     /translation="MDALIIKGTNETPNITFDKDANKFEITGKSLPEDVKEFYNPVLR
                     WLKAYAEDPNPKTVFKVKMEYFNTASSKMLLEVLELLDGMYSAGKDVEVEWHYMEDDE
                     DMHDAGNDYADMLELPFKMISYQHISK"
     gene            18909..19292
                     /locus_tag="PK049_05745"
     CDS             18909..19292
                     /locus_tag="PK049_05745"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOK61094.1"
                     /translation="MERLHLEPTLTTPKVIFDRDSGEFTIQGKSLPEDVKSFYTPLIA
                     WLDDYMKNPNKTSLFEFDFEYFNTASSKMILIIMNRIKELFKKGYEVQIVWKYPQQDA
                     ELEEAGEELAELISVPFKLVPKPEM"
     gene            19305..19718
                     /locus_tag="PK049_05750"
     CDS             19305..19718
                     /locus_tag="PK049_05750"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOK61095.1"
                     /translation="MEPLRIKARKDSPDITFDPQSGNFTIIGVSHPENVTSFYEPVVK
                     WLTQFKIEIQKNGAKSVKPINFRLFFKYINSASYKYMITLFQHMQEIFEMGVPIQIVW
                     NYEPGDEDMAESGWELIEYSGIKAPSVCEVCNDPI"
     gene            complement(19796..20890)
                     /locus_tag="PK049_05755"
     CDS             complement(19796..20890)
                     /locus_tag="PK049_05755"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOK61096.1"
                     /translation="MKRVLVATGGGDCPGLNAVIRAIVKRASQEPDWEVIGSIQSFDG
                     ILREPTEIKVLDERAVAGIHVQGGTIIGTTNKGGPFAWPVKNKDGSWGAVDRSDEMIR
                     KLQYLGIDAVISIGGDGSQRISQQLFEKGLNIIGVPKTIDNDLSATDFTFGFQTAVQI
                     ATEAVDKLVTTAASHNRVLILEVMGRYAGWIALHAAIAGGAEVCLIPEIPYDIHKVAE
                     RLQSRYNKGKGHAIVVVAEGALPKGGTLVSAESDEVGYHNLRLGGVAHKITRDLKSIG
                     FEADIRETVLGHLQRGGIPVAYDRVLATQFGVKAFEMVLEGKFGEMVAYRHPDIISVP
                     LIDAINRPNFVDPDCDLVRTARGVGISFGE"
     gene            complement(21353..24274)
                     /locus_tag="PK049_05760"
     CDS             complement(21353..24274)
                     /locus_tag="PK049_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOK61097.1"
                     /translation="MKDLEIKINELSDLLSCEVKWDESTLIQYSTDASAYKEKPYAVV
                     WPQDVNDIQQVIRFAKKHKIPVIPRAAGTSLAGQVVGKGIIIDISKHFRRFINLDIEK
                     RRVTVEPGVILDELNLFLKPHGLFFGPETSTSNRCTLSGMVGNNSCGSHSLVYGSTRD
                     HLYSVKMVLDDGNEYLFGEVNKTEYDEKLRLSNREGDIYRYIHNLIGNEQNQKLITSN
                     FPDPSVKRRNTGYALDTFIGTVAAGGELPLNLAKLIAGSEGTFGIITEITLNLVPLPP
                     KEKAVVAVHLKNRTDAFKANLIALKHKPWAVELMDNIILDCTKGNIEQQKNRFFVQGD
                     PGAILIVEFFADSKGEIESAAQKMESDMREAGYGYHFPIIWGAETSKVWNLRKAGLGV
                     LSNVVGDAKPVSLIEDTAVPVERLPEYMDDFEKIMQRYGLECVYHAHIGTGELHLRPV
                     LNLKDPKDVELFHTIGYEVAMLVKKYKGSLSGEHGDGRLRGEFIPIFYGEEVYQLMRE
                     LKFAFDPDNIFNPGKIIDTPPMNSSLRYIPGQKERVIDTIFDFSSSLGIIRATEKCNG
                     SGDCRKTHLSGGTLCPSYMATLNEANTTRARANLLREFLSYSPKRNPFNQRELYEILD
                     LCLSCKGCKNECPSSVDMAKLKAEFLQHWYDANGIPIRSRMVAYIAHINRMGSYFPRL
                     TNWFLSNKVTSKILMQAIGFEPKRKMPLLGESTLKKWFKRNRCHSAKHNGKVYLLPDE
                     FTNYNDVHIGIKAVELLNRLGYEVELVDIFESGRTYISKGLVRTAKRIANSNIEKLSG
                     IINPDTPLIGIEPSAILSFRDEYPDLVSQENKQKANSLAENSLLFEEFFIREAAKGRI
                     NPEMFTTRAQHIKLHGHCQQKAIASTTPTKAMLSFPVNYTVEEIPSGCCGMAGAFGYE
                     KEHYDLSMKVGELVLFPEIRKTPLDTIISAPGTSCRHQIKDGTGRKALHPIEVMWEAL
                     V"
     gene            24610..25821
                     /locus_tag="PK049_05765"
     CDS             24610..25821
                     /locus_tag="PK049_05765"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOK61098.1"
                     /translation="MIVLVLNCGSSSIKYQLLNMANDGQLLAKGIVERIGFTDAILTH
                     KPEGKDRYEVVTSIPDHTTGINLILEALVNEKHGVIKSLNEITAVGHRVAHGGEFFTK
                     SSLIDDFTKSEIEKCIELAPLHNPANLKGILSMEKLLPGVPQVAVFDTSFHQTMPKYA
                     YLYAIPHDYYDKYKIRRYGFHGTSHKYVAQKACRILGVDFESQKIITCHLGNGASIAA
                     IKHGKSVDTSMGFTPVEGLIMGTRSGDVDAGILLYLAEKENLSIKEVNDVINKKSGVL
                     GISGLSSDMRDLKNAAAQGNERAQLALDMYYYRVKKYVGAYAAAMDGVDLIIFTGGIG
                     ENDPILREKVSTAIDFMGIDFDSEANKGVRGKDKLLTKPSSKVKVMVITTNEELVIAT
                     DTANIVKELKK"
     gene            25847..26752
                     /locus_tag="PK049_05770"
     CDS             25847..26752
                     /locus_tag="PK049_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HOK61099.1"
                     /translation="MRVEKLDQLFDILRSKPRKRLVAAYANDHHTIEAVSMAIDMGIV
                     DATLVGDEATIKKVCGEHNIDFSKFRVVQEADEMKAARKAVELINKGEGDVLMKGLVS
                     TDKYMRAILDKENGLLPPKAVLSHVTVVQVPTYHKLLIVGDVAIIPAPDLNQKIAITN
                     YLIQTAHSLGIEMPKVALNAATEQMSAGMQACVDAAIISKMADRGQIKGAIVDGPLAL
                     DVAIDKESAQIKKVSGEVAGDADCILFPNIESGNVFFKACTKLAKGELGAMVMGAKVP
                     CVLTSRGDSVQSKMYSIALAANAAK"
     gene            26787..27863
                     /gene="buk"
                     /locus_tag="PK049_05775"
     CDS             26787..27863
                     /gene="buk"
                     /locus_tag="PK049_05775"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HOK61100.1"
                     /translation="MEEYKILAINPGSTSTKIAVFRNNKNIFLKNIKHSADDLAKFKT
                     IADQFSFRKEIILEELKQAGIDVNKISAVVGRGGLLKPIESGVYEVNEQMKVDLRNSI
                     MGEHASNLGALIADDIARSLPSAKAYIADPVVVDEMEDVARISGHPRFKRVSIFHALN
                     QKAIARLHAKSIDKKYEELNLVIAHLGGGISVGAHRQGRVIDVNNALDGDGPFSPERA
                     GTVPAGQLAKLCFSGEVTYDEVKKMLTGKGGLVAHAGTNDAYEIELKARAGDAKAKLL
                     QDAMAYNIGKAIGSMAAVLKGKVDAIILTGGIAHNPDLVDYVKEMVGFIAPVAVYPGE
                     DEMQALAMNGLMVLKGEVMPKEYK"
BASE COUNT         8115 a         5647 c         6162 g         7979 t
ORIGIN      
        1 gtaaagcact atgcgtgctt tttcaatttt ggcaaaagcc ctttcaatgc ctatgctctc
       61 aatggtatcg gtggtttgac gcaacccagc agtatcgata aagcggaagt tgataccgcc
      121 caaatggatt gtatcctcaa tggcatcgcg ggtggtaccg gcaatttccg aaacaatagc
      181 cttttcctcg ttaagcaaac ggttcagcag tgtcgatttg cccacattgg gcttgccggc
      241 aatggtaacc ggaatgccag ctttaatggc attgccctgg ctaaacgatg ccgatagctt
      301 atcgatttcc gaaatgatat tattaacaag tgtttccagt tcctggcggt tggcaaattc
      361 cacgtcctcc tcgctgaaat cgagttccag ctcaacaagg gcagtgaacc tgagcagctc
      421 gttacgtagc tcggtaagct tgttcgatat gcccccgcgc atctggttta gcgcaactcg
      481 ccgggctgtc tcgctggtcg atgcaatgag gtcggcaatg gcctcggcct gcgataggtc
      541 aattttacca ttcatgaaag cccttaatgt aaactccccg ggctgtgcaa gccgtgcgcc
      601 atggcgcaca agtagttcca gcagttgctg ttggataact gtggaaccgt ggcacgatat
      661 ttcaatcaca tcctcgcccg tgtagctgtg tggtgccctg aaaaggctta ccaaaacatg
      721 gtccacctcg cggtcaccat caacaatgga gccgtagtga atggtatagg ctttctgctc
      781 cttgaggctt ttcccctttt tggcaggacg gaaaacagaa ctggtaatct ctaatgagcg
      841 ttcaccgctt aaacggagaa cagctattgc acccattcct ggcggtgtgg ctatggcaac
      901 tatggtttgt gtggtatcga gtgtcatgaa ttgttccctt aaattggttc aggctataaa
      961 actttacaaa actggcaata acaattactg cttttaatcg tcattcatct aaaattatat
     1021 agtacaaagg taagcctttt aagccattat ggcaaaaagt ataaaaattg gttattataa
     1081 ggatggagta gcattataac ccaaaaaatc taaaagaatt gataattatt cttgatttaa
     1141 taatttaaat ccaatgttgt ttagttaagg ttttttgtca tgttacgcac aagcgaaaca
     1201 aaacgctgtc gtgcagagtc ccgatttata gggatagact tcgccatagc atctcattgc
     1261 tcttttcact tcaatccttt gaagagatcc ctcaactttg ctcgggatga caaaagccct
     1321 atcttccttt ctgctcactt gtcatgctga gctagtcgaa gcatcgcttt gcttaaaccc
     1381 tttaattctt gttgtttttt tggaaaccgc tctttgccaa acttttctgt tgcgatgcaa
     1441 cgtttcaccc ttgctctttg gaatgccatg gcgtgcatcg ctttacatgc gagagttcca
     1501 ttgggttaca aatgttgagc ccctacgggg caggaagcgt tgttcgttgt ttgtggaatt
     1561 gacctcaccc ccagcccctc tccttatatg agagggaaaa agcaattagg aaactttttg
     1621 catggctaaa gtcttccctc tcaggggaga tttagagggg tcatcaaggt tgttcgtgag
     1681 atacgacctc accccaacct tttccctgaa aaggaatgaa gcggaagaag cattcagagg
     1741 cataaaagcc tgacccggca atggggcggg ggtggacgtg gcggtaggct tagtaagcga
     1801 taccacatga gtcacgccgt aggcgtggcg aatagtatcg cgtggcagtt ccacccacgg
     1861 ggtacccatc gggtcgggct tttcagcctt gattttcttt ggttctttct tgtatcaaga
     1921 caagaaagaa cgtgaaaatc actctttgca atgtttttcc tttgcgatgc aatgtttcac
     1981 ccttgctctt tggagtgcca tggcgtgcat cgctatgcat gagggagtaa ctattgtgtt
     2041 acaaatattg cgcccctacg gggctggaag cgttgtttgt tgttcgtgaa gctattgcca
     2101 tgttgagcta agcgaaacgt ctcatggggt cgattagtga tccttcgctg cgctcaggat
     2161 gacaggcttg tatttttttg ttagctaaac gacattgaat ttaaatctac caaatcaaat
     2221 ctaaatgaca aaaattttta aacttattaa caataaacct gtctatcctt tgacaggttt
     2281 ttgttttttc tatttttgtg cgataaccta aaccaaacca aaaccatgaa cacttttctg
     2341 gaaagtttcg attacgttat tcagctttac aacgagtatg ttgggggtta cttgatatta
     2401 gccctgctgg ttcccacagg catttactac tcaatcagac ttaaattttt acaggtaagc
     2461 cattttaagc atgctatcga cttggttcga gggaaatata gcaagcacga tgatgtaggc
     2521 gatgtaagcc attacaaggc tcttaccacg gcgctttcag ccactgttgg aaccggaaat
     2581 attgttggag ttacccttgc aatctatttt ggcgggcctg gggctgtgtt ctggctttgg
     2641 gttaccggct tctttggcat gatgcttaaa atggttgaat gtacattggc gcaaaagttc
     2701 cgtaaaatta atgccgatgg cactgtttcg ggcggtccta tgtactacat tgaacatggt
     2761 cttaaggata agctgggtgt ttgggctaag cgtttggctg ttttctttgc ctttgctggg
     2821 attctttgtt cattaggaac tggcaatatg gctcaagcca attcaatctc cgatgtttta
     2881 aaaactagct ataatatccc tgttctttac accggtattg ccatttcagc gttagtgttt
     2941 ttaattgtag ttggcggatt gcgccgcatt gccaatgtta cctctaagct tgtgccttta
     3001 atggctgtta tctactttgg ttctgcaatt cttgtcatag gtgttcacta tgccgatata
     3061 ccttcagctt tatggttgat tgtaaaaagt gcttttacgg gccaagcagc agttggtggt
     3121 tttgtgggtt caaccttttt catgactatg atatggggtg ttcgccgggg tttgttttcc
     3181 aatgaggccg gacagggctc agcgcctatg gcgcatgctg ctgcaaaaac tccatacccc
     3241 atgcgtgagg ggtttgtggc ttcgcttgaa cctttcatcg ataccattgt aatttgtacc
     3301 cttactgcat tggttattgt tttaaccggc gtatggcgat tagatggtgc cgttaaaggg
     3361 gtagggatga cagttgctgc ttttcaggag ggcttgggta aaattggcat tggcttttta
     3421 gcgcgtcaca tggtggccat tagcctgcta ctttttgcgt tctctaccat cattagctgg
     3481 tcatactacg gaaccagaac tgcacagtac atttttggcg ataaggccat taaaccttac
     3541 tactacgttt ttaccctatt tgtattcttt ggttcagttt ggggtatcga tttggtttgg
     3601 cactttgtgg atatggttat cacctttatg agcattccaa acctgatagc gctactgctt
     3661 ttattcccag taatgaaagc cgaggccgaa ggctatttaa agcgacttaa agcaggggaa
     3721 tttaacttga acagagatta gcttaaaggg ctaggaaaat atctgcagtg cgctgtccgt
     3781 tgttcgcaaa atgaaatgga acacggatgg cgcacattag ttgatggttg acggttgata
     3841 gttgatagtt gatagttgat agttgatagt tgatggttga tagttgaaga ttgataatga
     3901 caaaaaacaa tgcgaaatgc ttatatatga ctgattacta aatatttgga tttttcataa
     3961 caccaaacat aaaacacaaa acacgaaaca ccttttactt ctattcccgc taacatagga
     4021 cgggtcaacc tttaaccttt aacctttatc ctttatcctt tatcctttat cctttatcct
     4081 ttatccttta tcctttatcc ttccctctac ctactccgcc agtgggcacg attccggttt
     4141 ggtgtagcgt ttacagcgcg gctgacaatc aatacaaagg tttatagcct cgcctgtttt
     4201 tgccttggtt acccaatcgg ggtcggttaa catgtcacgt ccaatggcta ccatatcagc
     4261 taagccgtgt gtgaccaact tattagccct ttcaggtgat tttatttggt taaccaccat
     4321 tacaggaaca tctacaaact tttttacctc tgttcccatg taaaccacat ggttgtagtc
     4381 aaaatttgag ggaactgtgg ggttaatacc cttttcgcca ccgtgcgata catgaaggta
     4441 atgaacgccg tatgcctgga gcagtttggc aatttcaata ccatcgttca gggtggggga
     4501 acaaccagcc atgcggtaac caataataaa tgttgagggt gtaacttcct tgataccttc
     4561 aatgatttgc cttgcaaatc gtaacctgtt ctcagagttc ccaccatact catcggtgcg
     4621 gtggttaatg gcagaagagg cgaactggtt aagcaggtaa ccatgggcac cgtgtagttc
     4681 aacaccatca aagcccgcta ggtgtgccct tttggctgcg cttacaaaat tttctaccag
     4741 ttcacttact tgttctttgg ttaaggctac ggaacggtcg ttttccgggt caacggaggg
     4801 accaaaggcc ttttcggcaa cagctttccg tgtggtaagt cctgcatggt gaagctgaat
     4861 aagaaccttg gcgtcgtgct tgtggacagc ctcagcaatt ctggcaagcc cctcaatgtg
     4921 gttgtcgtcc catatgccta actggtagct gaaaattcta ccagtgggat tcacacaggt
     4981 ggcttcaaca ataattagac cggtgccacc cctagcaatt ctttcgtagt gtgcaatatg
     5041 cttgctgctt acctttccat cggagtcgga ccatccaaag ttaacaatgg gtggcaaggc
     5101 aacccggttt ttaatattta ttccgttaat tacaattggc gtgtgaatat ccataataac
     5161 tttaattaac taatcacaaa tatagataat tagccgcata caaaaaaaat taaccaaatg
     5221 gttaaaaaag agactaaaaa atttggagca taaaaaaatg tgtcagatat ttgccgtatg
     5281 gtttaaccaa atggttaata ttgtatgaca ccaaaaaaaa tagagaaaga cactgagagt
     5341 ttgatacttg aagctgccaa acgcgtgttt gtccaaaagg gattggacgg ggcgcgtatg
     5401 caggaaattg ccgatgaagc aggaattaat aaggctctgc tgcattacta tttccgttca
     5461 aaggaaaagc ttttcatgac catctttaag gttgagctga acaacttttt tccacggctc
     5521 atacccgtat tactatctat ggagatgacc tatgagcaaa aaattcggca ttttgtggaa
     5581 gagtatatcg aacttttcct tagaaatccc tttctacctg cttttgtgct gcgcgaggct
     5641 aatcgaaatc ccgaaatgat tgcacagttc attactgaat cgggcatcaa tgccgaaaaa
     5701 ctaaaagatg tgatgaaggt tttatcccat gaattgggat taagcgtgaa cgaggtgctt
     5761 caccttatgg taactactgt tagctcatgt tttttcccgt ttgccggtaa accaatcatt
     5821 gaacaggtgc tttttaatgg caacgagggc gattaccaaa agtttttgag tgaacgtaaa
     5881 aggtatgttg ccgactttat gatttacttt ctaaaaggaa gattaaaatg attttaacaa
     5941 tgagaaaggc aagaattatt tcgcttacca ttactttact ggcttcaacc tatatgctaa
     6001 agagccagaa cacggttact ctgccgggct tacttaaaag catggagcag tggaataagc
     6061 tttacagggt tagtgcggca accgactcgg tttactggct taggaagtcg aatatcaagg
     6121 taaactactt accaaaagta gaattgaatg ccgaggctac ctggcaatcg gatgtaacaa
     6181 aagttaacat tcccatgtcg ggcatttcaa tccctacacc cgacaacgac aactataagt
     6241 ttactatcga tgttagccag ctgatttggg atggaggtgc aacaaaatcg atgcatcagc
     6301 ttgaagatga atcgcgtaaa cttgagcaga acaaggttga aagcgaactt tacacccaaa
     6361 aggaaagggt ggcttcgctt tactttggaa tactttccac cgatatggca atcaggcagc
     6421 ttaaagctat ggccgtggag ctcgataagc gaatagaaga gcttgaggca ggagtaaatg
     6481 tgggcgtagt gctggaatca tcagcaaacg ctttaaaggc tgagaggctt aggttggagc
     6541 aaagcattga tgcaaacctt tcgcaacggt atagtttgac agaatcaatt tactcactta
     6601 cgggagtaaa aattgatgag agtgcccaag ggattatacc ctcattagct ataccaaacc
     6661 aggatgtttg ttcacgcccc gattataaac tttttgacct gcagaacggc tacctgtcaa
     6721 cagcagcagg tgccttgact agcaggcgaa tgcctaagtt aattgcattt gcacgggttg
     6781 gctatggcaa accgggactt aacatgcttt ccaatgagtt tgattccttt gccttggttg
     6841 gagcccgatt atcatggagt atatgggatt ggaatagcac atcgcgcgag aggcagaccc
     6901 tgaaaatcca acagaacatt ttgcaatata ggcgcaatgc ttacgatgaa ggatgtagtg
     6961 cgcaggtttc atcagcgcta gcgcagatac aatcgttaca aaggcaaata caaaccgatg
     7021 aaaaaatagt tgagctgcta gaaaagtcag taaaggcatc agcatcgcag cttaaaaacg
     7081 gaaccataac atcatcggtt tacttgtccg acttcaatag cttgcttaga tcaagaattg
     7141 atatggactt acataaaata aagctctcac aggaggttgt aaagctatat ttcacacttg
     7201 gcatgagtat taacgattaa aaaattatac caatgaattg gaaaaatctt atcaacgtaa
     7261 ttcttcccac tgcaattgtt atttcatgta gtagggataa tggaaaagct gatgcttacg
     7321 ggaacattga atcgaacgat gtaaccattt ccgcagaagt gggcggtaaa cttctggaac
     7381 ttaaaatcga cgagggatat aaagtagaga aaaatcaact tattggaatt gttgatactt
     7441 taccgcaagt ccttaaacta aagcagctta atgcccagct aaaggctgcc aaggcaaagc
     7501 tgcaaggtat tgatgctcaa attgccgttc aggatgagca gctgaacgtc ctgaaaaagg
     7561 aacttgatag ggtaaaaaaa ttagtagccg atagcgcagc aacactgcgt cagctcgatg
     7621 atattgaggg aaagttcaaa attgccgaaa agcaaaaagc aacgtttgat gcgcaacgct
     7681 caggggtgtt ggcagaaatt gacgctgtag atgctcaaat tgcccaggcc caggatttat
     7741 tgtggcggtg ccacatggtt tcacctgttt cagggacagt aatatcgcgc tatgtgagcc
     7801 agggagagct ggttattacc ggcaaaccgg tttgcaaaat tggtacgctc gatacggttt
     7861 acgctagggt ttatattgat gaaactcaac ttcccgattt ttccattggc cgtaaagtga
     7921 caattttaac cgatacgcct gatggtaagc tcaaggagga taaaggcgaa attgcatggg
     7981 tttcatcgga agctgaattc actccaaaaa ttattcaaac aaggaacgaa agggttaacc
     8041 ttgtgtatgc tgtaaaagtt cgagttccca acccaaatgg ctattttaag ataggaatgc
     8101 cagtggaggt taggattgaa gattagtgtg cattgttttg tgttctatgt gaagagaatg
     8161 atagacagtt atgtttttgg ggtttacttt tttatctact tcttcctgac actagttttt
     8221 tgctgaacat tgacgtttaa ttctaaactc tatctaaatg cctgcaatac aagtaactaa
     8281 catatcaaaa tcatacggca aggtaaaagc gcttaccgat gtaagtctga gcgttgagga
     8341 gggtgagatg tttggtttaa ttggccccga tggagcagga aaatcaacgt taatccgtat
     8401 tattacaaca ctgttagttc ccgatagcgg gaatgtaacg gtttttggtt tggataccgt
     8461 aagccagtat aaactgataa ggggcgaaat tgggtatatg cctgctgttt tctcacttta
     8521 ttccgattta accgtggaag agaacttaaa cttttttgcc ggggtttttg gaacaacagt
     8581 aaaggaaaat tacgaactgg taaagggtgt atatcacatg cttgaaccat ttagaaatcg
     8641 ccctgccgga aagctatcgg gtggtatgaa acaaaaactt gcactttcgt gcgcattgat
     8701 tcatcgccca agggtgctga tactcgatga gccaacaacc ggagttgacg ctgtttcaag
     8761 aaaggaacta tgggatttgt tacattctat taagaaacag ggtattactg tttttgtttc
     8821 aacagcgtac atggatgagg caaagcagtg cgatagggtt gcattgattc agaaagggcg
     8881 aattttagga actaacactc ccgaagggtt tgctgaaatg tttccctata aactctttag
     8941 cattcaggct aacaatgcat ggagtagttt agatgccatt aggcatttaa gtacatgcga
     9001 aaccgcctac atgtttggcg atagtattca cttcgcaact aattacaatg aggaaagtcc
     9061 tgaaagcctt cacgatagat tgatgtcggt aggagttgaa gctaatgtag ctcaaataca
     9121 ggcaagcatt gaggattatt tcatttacca actctcggaa aaaaatcttg tgaaatgaca
     9181 aatgccattg aggttgaaaa cctcgtaaaa cgctttggaa gttttattgc tgtaaatcac
     9241 ctaaccttta gtgttggtaa aggggagata tttggactac ttggagccaa tggtgcaggt
     9301 aagaccacca caatcaggat gctttgcggg ctgctttcac ccacatcggg gaaaattagc
     9361 gttgcgggct atgatgcata cacgcagcct gaacaaatta gggccagcat tggctatatg
     9421 agccaaaagt tttcgcttta cagcgattta acggtttggg agaacatacg attttttggt
     9481 ggtatttacg ggttatgggg aaaagagcta agaatacgtg cacttgaact tgtggaggag
     9541 ttcaaaatgc acgattttaa agacactaag gttgaatcgt tgccggttgg gtggcgacaa
     9601 agaatttcgc tagcggtatc gttagtgcat aagcccccaa tcatcttcct cgatgaaccc
     9661 acaagcggtg ttgacccggt tggacgacga atgttctggg aaaccattca tcaaatctcc
     9721 tctatgggaa caaccgtgct ggtaaccact cattacatgg acgaagcaga gtactgccat
     9781 actgttcttg tcatggttga tggaagaatt gaagctcagg gtgcacccgc gcaactaaaa
     9841 tcgacacatg gagttgaatc aatgaacgac ttgttcttac tactatcccg aaatggtaaa
     9901 ttaaacattg agtagctatg gcaaagggta ggttttcgaa ttttattacc aaggaattta
     9961 agcacatatt tcgcgatacg cgaacgctgc ttattgtgct tgtaatgcct gtcctgcaac
    10021 tgctaatttt tggctttgtt atatccagtg aaatcaagaa tgccaattac gccgtgcttg
    10081 actacaccaa cgattataac acaaaaaagt tagtgcagcg tatttcatca tcggggtact
    10141 ttaattttaa gggatatctg aaaagcgaaa atgaaattaa tgaagttttc aagaatggcg
    10201 atgccaagct ggttcttatt attggtaaca atttcactca aaagcttcaa aatgaaaact
    10261 ttgccaatta tcaagttgta accgatgcct cagaccccaa tacagcaagt attctggcat
    10321 cgtatgcaca aggaatagta aattcgttta ctgcggagta ttttggttca agccagcaaa
    10381 aggagagggt aaacgttcaa agccgaatgc tgtataatga ggaaatgaac tcggcattca
    10441 tgtttgtgcc aggaaccatg gtacttatac taatgttagt tactgcaatg atgacctcta
    10501 tttcaattac tcgtgagaaa gagatgggta ccatggaaat actgttggtt tcaccattaa
    10561 aaccttggca gattgtggta ggtaaagtaa ttccttatat aggcattgca tttaccaatg
    10621 ttctggtaat cattttaatg ggctacacag tttttcatct gcccttaagg ggaaacttcc
    10681 ttttgctgat gggggttagc tcgttgttca tagtgttagc cctatcatta ggaattttaa
    10741 tatccacttt gagcaatagt caggctgttg ccatgttcat gtcaatgttt gcattgatgt
    10801 tgcccaccat tctcctctcg ggattcattt tccccattga aaacatgcca aaaattctgc
    10861 aatggttaag cctgattatg ccaccacgat ggttcctttc ggcattaaaa acaataatga
    10921 taaaagggca aggcttacaa tacgtaataa gcgaagtgct ggtaatgcta accatgacaa
    10981 ctgtttttat agcccttagt atcaaaaagt ttaaagttcg tttggagtaa tacagcttgc
    11041 tatgagaagg atactaatac ttttacagaa agagtttatt cagattttca ggaataaaac
    11101 tatactacca ataatattcg tattgcccat cgttcagctg ctcatcctgg ctaacgcagc
    11161 tacacaggag atgaaaaaca tcaggttgac catagttgat tttgataatt catcggaatc
    11221 gcgcaggcta ataaacgctt ttgaccaatc aaaattttat accttgatac ctgttagcgg
    11281 caatgttgac agggctgttg aactaatcga tttaggtaaa accgatgctg cactggtaat
    11341 cccaaagcgt tttggggaaa gcctagttga gggggcaccc gtaaatattc agcttatgct
    11401 gaatgccatt aacattacag cggcagcatt aacacagagt tataccaccg gaattattca
    11461 gaacttcgca tccagcattt caatacaaaa tataaatcat atcaaaccta aacaaagcgt
    11521 aaggtattgg tataatccag aactgaacta taagctttac atggttccag gtattcttgt
    11581 aattttagtt accattgttg gcatgttctt aacggcaatc aatattgtta gggagaaaga
    11641 gcttggcacc attgagcagg ttaacgttac ccctattaag ccctatcagt ttgtaattgg
    11701 caagttactt ccattctgga taattgcgtt atttgaactg tcgtttggtc tactaattgc
    11761 caggcttgta tttaacctaa ccattgcagg cagcattcca cttctgtttg cttttgctgc
    11821 aatatacctt ttggtagcac tgggattagg tttgatactc tcaacatcag ctagcaatca
    11881 gcagcaggta atgtttatgg ctttcttttt catgattgtt ttcctgatgc taagtgggtt
    11941 gttcacccca actgagagca tgccaaaatg ggcaaactgg tttaatacca tcaacccgct
    12001 atactacttt atgaaggtaa acaggatgct cattctaaaa ggctccgatt ttacaaacct
    12061 gaaagatagt tttctatcaa tcagtctaat ggcagttgca gttctaagca tagccatact
    12121 gcggtataga aaaaaatcag catagtagcc gaaataattg tatttttaca ggagaaccat
    12181 ttcaatgagt ggtgctaaac agaattgcat caaatactat ttttttggta ttgctacttt
    12241 ttagcaattt agcagatggg caacctgtat ttgccgatac ttcattgctt gatgttaaac
    12301 ttgaatttgg cattcagcaa gtttttgccg atataggcga agacccatcg taccataaag
    12361 gatatttaag ctatgagaac gaaaatggcg ctactattag tattcccgtt gagataagga
    12421 ctcgtggaat tttccgacga aaagcatcaa attgttccca accaccgctg ctcataaaat
    12481 ttaaaccaaa agagacctca aataccattt ttgagggtat tgaaaaacta aaattggtgg
    12541 taccatgcca aaaatctagc aaatacgaag atttagtcct aaaggagtac cttgtttaca
    12601 agctttacca gattatttcg ccctattcat acagagtaag gcttttaagg ttgaatatgg
    12661 tagatagcta ctatggcgac cagtcaattt gttatgcctt tgtaattgaa ccggttgaaa
    12721 tactttcaag aaggttaaga ggagtggtta gggatgctaa aaacactcac ccaaacgcct
    12781 gtaataacta ctactacaat aggatggcca tttttcagta catgataggc cataccgatt
    12841 ggtcaataaa ggcattgcac aatatcaccc taattgaacc cgaacccttt gctccatcca
    12901 ttcccgttcc ttttgatttc gacttttctg gctttgttga tgctccttat gcgcttcccg
    12961 ctgagcacct acccataaaa tcggtaaaag agcgacactt caatggctac tgtaagccgg
    13021 aacagcagtt tgctgatgcc ttcagctatt tccttaacct aaaggataca ataaaccata
    13081 ctattaccac gtttaaatat ttaccccagg cgcaaagaaa tgagctaatt aggtatacca
    13141 atgaattctt cgatataata gcatcggata caaaacgtaa atcaaggatt attacaaaat
    13201 gtcgaaccga ttagtcaatt tattcgtatc actttcaaat atttttactt aatttgttag
    13261 aacttttgtt attttgcaat tgcttggact gaaaatgcag aatatgtcgc tcatgaaatc
    13321 aataatatca aaggcttttt ttacaatatc ggttattatc ttactttgcc tatcgtccat
    13381 tgctcagaat ggattgcagg tatcggctag gctaatagtt aaaaagggcg atttacaggg
    13441 ttcaaaggta acagtaaccc aaaacggaaa tgtagttcaa accatatcgg ttagcaataa
    13501 aggggttttt gattttattc ttgagtataa ctccgagtat atcatagttt ttgagaaacc
    13561 tggctatatc actaaaaagg ttttaattga tactcgtgtt cctgaaacat tccgtaagga
    13621 tattcagcaa atgaccggat ttgatgtggt tcttgaaaaa cagagtgagg acgttattaa
    13681 ggagtatgcc aatcctgttg caaagataat gtatgcaagg gatataagag actttaccta
    13741 cgataccgac tactccctta aagtgcttaa agaaattgaa aatgaaaaca aaaagttagc
    13801 tgaggagaaa aagaagaagg aggaggaaga aaaagcccga aaggcagagg aggaaaaaca
    13861 acgcatttta gctgaagccc aaaaacgtaa agaggaagaa gaggctaagc gcaaagctgc
    13921 tgaggaggca agaaaaaagg aggaggaaag gttaaggaaa gaggctgaag agcgacgcat
    13981 tgcagaggaa aagaaaaggt tggaagagca aaagcgtctg gaggaactta agaaaaaggc
    14041 cgaggaggaa gcacggttaa aagctgagga ggaagctcgt aagaaagagg aactcagaaa
    14101 acaggaggag gaaaggctga gaaaagaaca ggaacgtatc cgtcaggagg aggaagcaaa
    14161 acgtaaagct gccgaggagg ccagacttaa agaagaggaa aggctgagaa aggaagccga
    14221 ggagcgaagg ctggcagagg aaaaagcaaa acttgaggaa caaaaaagat tggaagagca
    14281 acgcaaaaaa gctgaggaag aggcgaggtt aaaagcagag gaggaagccc gtaagaaaga
    14341 agaattgaga aagcaggagg aggaaaggct taaacaggag caagagcgtg ttcgacagga
    14401 agaagaagct aaacgtaagg ctgccgagga ggctcgttta aaggaagaag aacgcattcg
    14461 caaggaggct gaggctcaac gattggccga ggaagccaaa cgtaaagccg aggaagaggc
    14521 aaaactgcaa gaggctgaaa ggttaaagaa agaggctgaa gaaagggcta ttgccgaagc
    14581 taaactacgt gaggaacgtc tgaaggcaga acaggaaaaa gctagacagg aagccgaacg
    14641 gctaaaacgt gaggaggagg aacgccgtag agcagaggag caaaaaaagt tgctccttga
    14701 ggagcagcgt aagcgcgatg ctgagttacg ccttaagctt gtggccgaga aaatgagcgc
    14761 ccaaattggc aaggaggatg ccgatcaggt tgtaaccctg cttgctgccg aggatattgc
    14821 caaacgatat acccgtgaac gtaccgatga ggttatccgt tatccacgaa taatacttac
    14881 ccgaacagtg tttaaccgga acaacacaat caccttctac actcgtgtcg accatgagta
    14941 tggtgcagtt tactacctaa aggatggttt ctttatttcc aaggagcaat accttgccga
    15001 aacccaaaag taaagttaag tgaaggagtt catcactgct tgcccacgaa actgctacag
    15061 cacatgtagc cttgttgtgc aggttgataa gggaaaagtt gtaggtatca accatttacc
    15121 cttaaataag gcaactcctt ttgggggatg ccttaaaggt ttaagctatg tggaacgagc
    15181 aaattcaccc gatcgtatta tttatccact caaaaaggta aatggttcct ttaaacgcat
    15241 ttcatggaac gaggctcttg aaactattgc tgaaaagctg atacatttcc gcgatgttta
    15301 cggtcaacaa tcggttttgt tttacgctgc cagcggcatg gccggcttgt taaacgattt
    15361 cagcggaaag ttctggcgtt atgtttacgg aggggctaca accgtttacg gtaatctttg
    15421 ctggcctgca ggtttggagg ctacgcgctt aacgcttggt gaaaataagc acaatgttcc
    15481 ttgggattta gagaatgcta agctaattgt tctctggggg aaaaacccag ctgagagcaa
    15541 cgttcacgaa atgatacccc tgaacaatgc gcttgataag ggggcggaac ttgtggttat
    15601 cgatccacgt agaacccaat caacccaaag agctactttg ctgctacagc catacccatc
    15661 aaccgatgca cctattgcat tggctgttgc caacataatt attcaaaacg gatggattga
    15721 tagtgatttt atcaaactcc atgttcatgg ttttgatcag tttgctgaac atgttaaacc
    15781 ctttacggct caatgggcat ccaatatatc gggggtgcca gttagcttaa ttgagaagct
    15841 ggcatggtac attggcaatg taaaaccaat gacattaata ccaggttatg gcatgcagcg
    15901 atatacaaac gggggacaga ctgtaagggc aatacttgcc cttagtgtaa ttacaggtaa
    15961 tattgggaaa tccggcgcat gctggcacta cgctaacctt caaagttatg tttttgacga
    16021 tgtactggag ccactctgtt actatccgcc cgataaaccc gatggcgtta tacgtcgttc
    16081 catttcaact gcgcggttag gagaggacat gcttgccact aaaaatcctg aactaaaaat
    16141 gatttgggta gaacgcggaa atccgctaac ccaaaaccca aatacgggca aagttcttga
    16201 ggccttccgg aaactcgatt tcagagtggt ggtggagcaa ttcatgaccg atacggctct
    16261 ggaagccgat attatcctac cggctaaaaa catgtttgaa caatccgata tagttggttc
    16321 gtactggaat ccttacattc agctcaagca aaaagtggtt gaaccagccg gagaggttaa
    16381 gcccgaaacc gaaatttact ggtggcttgc acaaaaacta gggtttaatg aggccgagtt
    16441 atccaatatt ttacttcccc cgggcgatga acatgttgat aggtaccttg atagtaagct
    16501 gcgcgagttt aacttatcgc tcgaagagct caggaaaggc ccaatacttg ctcccggtct
    16561 tcaggaaatt gcattctccg attatcgatt tccaactccc tcggggaaaa ttgagttgta
    16621 ctctgagcag gttcaaactt tatggggtga aaatccactt ccaacctata aaccagccgt
    16681 agagctattc cagaaccaaa cgccatatcc gcttttttta atgtcgccaa acaccaaaaa
    16741 ccgaatacat agccaattca ataatttaaa agtgatatca atgattagtg gcgcaccggt
    16801 attacaaatt aacccggctg atgccattca aaggggaata ggcgatggcg atctggttga
    16861 aatttttaac agtaggggtt cgttcagggt taaggcacag ctaacctatg aggtaagggt
    16921 gggctgtgtg gttatcccaa acggatggtg gagaacacag ggtgcaccgg tgaatgtcct
    16981 ttcggaaggt agagaaaccg atatgggaca cggatcagca tttcataaca atagggtaga
    17041 ggttaagctc gtaaaacgta aaccatgagc gacagggggt tcatattcaa ttattcccga
    17101 tgcgtggggt gtcatgcatg catagtggca tgctataacc aaaaccatac cgaaccccca
    17161 atggcatggc gaatggtggt taatggcaac cccttaaaaa ttccattaaa gggtttcatc
    17221 aacctttcat tagcatgtaa ccactgtatt gatgcacctt gcatgactaa ttgccctgca
    17281 attgcatatt caagggacga tgaaactgga gcaataatac acagccctct aaaatgcatt
    17341 ggctgcaagt actgtacctg ggcctgccct tatgaagcgc ctaaatataa cccgggtaag
    17401 ggtgtggtgg aaaaatgtaa tttctgtaac gatctgctaa aggttggagg catacctgct
    17461 tgtgctgcag catgtccaac aggcgctctc acctttggtg aaataaaggt agaggctaat
    17521 ctatcaaaac ccgggttccc tgaagtcgct acttcgcctc gcattaatgt agctaatgaa
    17581 agtatcaacg atagtttgcc cgaaatggct attgaggcca cgggccttca acaatccgat
    17641 tttgctgaga catatagccc ccgtattcat cccacaaagg aaatcccttt gtttgttttt
    17701 acttcccttt cagctgtatt agtgggatgg tttatcacct tttatcgcct tgagaaaatc
    17761 tcatactttt ctaagatttc cttcatttta ttacttgctt ttgcgggttt tgcaagttta
    17821 ttccatttag gcaagcctct aagggcgata agagccctgc ttcatgttaa atcgagctgg
    17881 ttgagtcgtg aaattgctct ttttggtttg tttgcctttt caggtctgct ttacgtttta
    17941 actgaaaaac ctccattatt ttggttttcg gtggttgtag gaacggtttt tctcatctcc
    18001 cttgaaatgg tttaccacgt ggttcgtaaa aactatagca cccctataca cagtgcaaat
    18061 actttgctaa ccgcctctac gctattttcg ttaataactt tgtcaaaagc ctttgttctt
    18121 cttgcaacca tcaaactatt gctttacctg gtacgccatg catacattca aaaattcaac
    18181 cctaaaaagg taattttttc atttataaga tttttaggta ttctcatacc acttgtagga
    18241 ttactcttta atttgattcc tgcaaacata gccccatttc tcaccctctt tcttattggg
    18301 gagtttattg accggtacga gtattacgct agcatcgata cccataaccc ttttcagagc
    18361 atttaaacct taggttgttt taaggaattt ccccggcgtt ttgtcaaaat tattttcaaa
    18421 caataacttt ttgctattga tattttgggt aaaatgttta tatttgtttt attaagttga
    18481 aaagtgattt ttataaacat agcagtatgg atgcattaat catcaaaggg actaacgaaa
    18541 ccccaaatat tacattcgac aaggatgcca ataagttcga aatcacaggg aaatcgctac
    18601 ctgaggatgt taaggagttt tataaccctg tacttaggtg gttaaaagct tatgccgaag
    18661 atccaaaccc aaaaaccgtt tttaaggtaa aaatggagta ttttaatact gcatcatcaa
    18721 aaatgctgct tgaggttttg gaattgcttg atggaatgta ttcggctggt aaggacgttg
    18781 aagttgagtg gcactatatg gaggatgatg aggatatgca cgatgctggt aacgactatg
    18841 ccgatatgct tgagttgcct tttaagatga ttagctacca gcatatttcc aaataacttc
    18901 taacgtccat ggaaaggctt cacctcgaac caacacttac cacacccaaa gttatttttg
    18961 accgggatag cggtgagttt actattcaag ggaaatccct tccggaggac gttaaatcgt
    19021 tttacactcc acttattgct tggttggacg attacatgaa aaaccccaac aaaacctcac
    19081 tctttgaatt cgattttgag tattttaata ctgcttcctc aaaaatgata ttgattataa
    19141 tgaaccgcat caaagagctt tttaaaaagg ggtatgaggt tcagatagta tggaaatatc
    19201 ctcagcaaga tgctgaactt gaggaggcag gcgaggaact tgccgagttg atatcagtac
    19261 cttttaaact tgtaccaaag cctgaaatgt aaaaacatac cataatggaa ccattacgca
    19321 ttaaagccag aaaggattcc ccagatatta catttgatcc ccaaagtggc aatttcacta
    19381 taattggggt tagtcatccg gaaaatgtaa ccagttttta cgaacctgta gtaaagtggc
    19441 tcacccagtt taaaattgaa attcaaaaaa atggagccaa atcggttaag ccaataaact
    19501 ttaggctttt cttcaaatac attaactccg cttcgtataa gtatatgatt acccttttcc
    19561 agcacatgca ggagatattt gagatggggg taccaatcca aatcgtttgg aactatgaac
    19621 ccggagacga ggatatggct gagtcagggt gggaattaat tgagtattct ggaattaaag
    19681 ccccctcggt ttgcgaggtt tgcaacgatc ctatttagaa gtctgaaagt acgctgaaac
    19741 ttgaaaaaaa aggggaagtt taaacttccc ctttcctgtt attgataaat actttttact
    19801 caccaaagct tataccaaca cctcgagctg tgcgcaccag gtcgcaatca gggtcaacaa
    19861 agttgggacg gttaatggca tcaataaggg gcacggaaat gatatcgggg tgacggtatg
    19921 caaccatttc gccaaattta ccctcaagca ccatttcaaa ggcctttacg ccaaactgag
    19981 tagccaaaac tctatcgtaa gctacgggaa tgccaccacg ctgcaagtgt ccaagaacgg
    20041 tttcgcggat gtcggcctca aaaccaatgc tcttaaggtc tcgtgtaatt ttgtgcgcaa
    20101 ctccaccaag tcggaggtta tggtaaccaa cctcatcgct ttcagccgat accagtgtgc
    20161 cacccttggg tagagcaccc tcagctacta ctacaatggc atgtcctttc cctttattgt
    20221 aacgactttg gagtcgttca gcaaccttat ggatatcgta tgggatttca ggaattaagc
    20281 aaacctcagc accgccggca atagcagcat gcagtgcaat ccacccggca taacgtccca
    20341 ttacctctag aatcagaact cggttatggc tagcggccgt ggtaaccagc ttatcaacgg
    20401 cttctgttgc aatttgaact gcagtttgga aaccgaaagt aaagtcagtt gctgaaaggt
    20461 cgttatcgat ggttttggga acaccaataa tattcaaccc tttttcgaat agctgttgtg
    20521 aaatgcgttg agaaccatcg ccaccaatac tgataacagc atcaatcccc agatattgta
    20581 gtttgcggat catctcatcg gaacggtcaa ccgcacccca tgagccatcc ttatttttaa
    20641 caggccaagc aaaggggcct cccttattgg ttgttccaat tattgtacca ccctgtacgt
    20701 gaatgcctgc cacagcacgc tcgtcgagca ccttgatttc agtaggctca cgtagaattc
    20761 catcgaacga ctgaatacta ccaataactt cccagtcggg ttcctgagag gcacgtttaa
    20821 caattgcccg gatcaccgca tttaatccag gacaatcgcc gcctcctgtt gctactaaaa
    20881 ctcttttcat tggttttaat ttcgtttgtt aaggtttttt acgctaaagt ctttgtttta
    20941 aaaactgaag taaaactgcc gaaaaagtta caaattgtaa tcattttttc gatatacctc
    21001 aataattaaa actggttgtt catgtttacc tcattatttg gtatattaca aattgtaaaa
    21061 agccaaattt tttccacgtt cgcaaaggta tgaatttttt taaacgtaca atgctaaaaa
    21121 gtatcaaaac tcttgatgtt acaaataatt atgcaccgca aacgtgtgaa aacgggaagg
    21181 ggaagaatcc gaataattag tatgaaacta aaaatcagaa agcagaaatg ctgagatagt
    21241 tctgaaatat tgcaaagtgg attgttttaa cccctttaac cttaaacttt gaacctcaaa
    21301 tctttaacct ttatccttta ccctttatcc tttacccttt atcctttatc ctttaaacca
    21361 acgcctccca catcacctca atggggtgaa gggcttttcg gccagtacca tctttaatct
    21421 ggtgcctaca gcttgttccg ggtgcagaaa ttattgtgtc aaggggtgtt ttacggattt
    21481 cggggaacag aactaactca cccactttca tcgataaatc gtaatgttcc ttttcataac
    21541 caaatgcccc tgccatgccg caacaaccac tcgggatttc ctccactgta tagtttacag
    21601 gaaacgatag cattgcttta gtaggtgttg ttgaggcaat tgctttttgc tggcagtgtc
    21661 catgtaactt aatgtgctgg gctcttgtag taaacatctc tgggttgatt ctgcctttag
    21721 cagcctccct aataaaaaat tcttcaaaaa gaagagagtt ctctgctaaa ctatttgcct
    21781 tttgcttgtt ttcttgggat accaaatcgg gatactcatc tctaaaacta agtattgccg
    21841 aaggttctat tccaataagg ggtgtatctg gatttatgat acccgatagc ttttcaatgt
    21901 tgctgttggc aatacgcttg gcggttctta ccaaaccctt tgaaatatag gttctaccac
    21961 tttcaaaaat atcaaccagt tccacctcat aaccaagccg attaagcaat tcaacagcct
    22021 taatgcctat atgtacgtcg ttgtagtttg taaactcgtc aggtaggagg taaacctttc
    22081 cgttgtgttt agctgaatgg catctgttcc gcttaaacca tttcttaagg gtagattcac
    22141 ctagcagggg catttttcgt ttaggttcaa atcctattgc ctgcatcaga atttttgatg
    22201 ttaccttatt gcttaaaaac caatttgtta atcgggggaa gtaactaccc attcggttta
    22261 tatgggcaat gtaggctacc atcctacttc gtatggggat gccgtttgca tcgtaccagt
    22321 gctgtaagaa ttcggcttta agctttgcca tatcaacgct cgaggggcat tcgttcttac
    22381 atcccttgca tgagaggcat aaatccagta tttcgtatag ctccctttga ttaaatgggt
    22441 ttcttttagg gctgtatgat aaaaattccc tgagtaggtt agctcgtgcg cgtgttgtgt
    22501 tagcttcgtt aagtgttgcc atgtagcttg ggcaaagtgt tccgcccgac aaatgtgttt
    22561 ttcggcagtc gcctgaacca ttacattttt ctgtagcgcg tattattcca agcgaggacg
    22621 aaaaatcgaa aatggtatca ataacccttt ccttttgacc gggaatatac cttaaagagc
    22681 tgttcatggg tggggtatcg atgatttttc ccggattaaa aatattatca gggtcaaaag
    22741 cgaacttcag ctctcgcatg agctggtaaa cctcttcgcc gtaaaagatg ggtataaatt
    22801 cgcctcgcaa tcttccatcg ccgtgttcgc cgcttaaaga acccttgtac tttttaacga
    22861 gcatggccac ttcgtagcca atggtatgga atagctcaac atccttagga tccttaagat
    22921 taagcacggg ccttaaatgg agttctcccg ttcctatgtg tgcatggtaa acgcattcaa
    22981 gcccatatcg ttgcattatt ttttcgaaat cgtccatgta ttcgggtagc ctttcaacag
    23041 gaaccgcagt atcttcaata agcgaaacgg gttttgcatc gcccacaaca ttcgataata
    23101 caccaagtcc agccttacgc agattccaaa cctttgaggt ttcagcaccc caaataattg
    23161 ggaaatgata accgtagcct gcctcacgca tatcactttc cattttctgg gcagcgcttt
    23221 caatttcgcc tttactatcg gcaaagaatt caacaataag tatggctccc gggtcgccct
    23281 gaacaaaaaa gcggttcttt tgctgctcaa tattgccctt agtgcaatcc aggataatgt
    23341 tgtccattag ttccacagcc cagggtttgt gcttcagggc tataaggttt gccttgaagg
    23401 catcggtgcg attttttaaa tgtacagcga ctacagcttt ttccttaggg ggaagaggta
    23461 ccaggtttag ggttatttcg gtgataatgc caaaagttcc ttcactcccg gcaatcaatt
    23521 ttgcaaggtt aagcggtaat tcaccaccag cagccactgt tccaataaac gtgtccaggg
    23581 catagcccgt attccgacgt tttacactgg gatcaggaaa attcgaagta attaactttt
    23641 gattttgctc attacctata aggttatgta tgtaacggta aatatcccct tcgcgatttg
    23701 aaagccttag tttttcatcg tattcggttt tatttacctc tccaaaaagg tattcatttc
    23761 cgtcgtcgag taccattttt actgagtaaa gatgatcgcg ggtgctcccg tataccaaag
    23821 aatgtgagcc gcagctgtta ttgcccacca tacccgagag ggtacaccga tttgatgttg
    23881 atgtttcagg gccaaagaat aaaccatgtg gtttaagaaa gaggttaagc tcatcgagta
    23941 taacaccggg ttcaacggta acccttctct tttcaatatc aaggttgatg aatctgcgga
    24001 aatgctttga gatatcaata ataatgcctt tgccaactac ctgtcccgcc aacgaggttc
    24061 ctgctgcacg tggaataact ggtattttat gctttttggc aaagcgaata acctgttgga
    24121 tatcatttac atcttgtggc caaactacag catagggttt ctccttgtat gccgatgcat
    24181 cggttgaata ctgaataagc gtggattcgt cccattttac ctcgcagctt agcagatcgc
    24241 tcagctcatt tattttgatt tccaaatctt tcatacctct ttaaaaattc atacaaactt
    24301 aaagcaattt atttttctat gattttcttc ccgtaatttt gattctaatg atttgatttt
    24361 gaaaaaatat aaaactgggt ataacatcgt aagcaattca agaaaaaaaa ataccatgtt
    24421 gaaaaacata tgctacatat tgcaatgtaa ttcagagtgt tagaggtgtt ttaaacgcct
    24481 gttttactat aaatcaacca gttaatgctt taaaaagcct taaatgcttt gaaggtgaag
    24541 gtatttattc ctaattttgc gtatgtaaat tgaattaact aattgtttga ctatcaaaat
    24601 aataaaacaa tgattgttct cgttctgaac tgcggaagtt catccattaa gtatcagctg
    24661 ctaaacatgg ccaatgatgg tcagttacta gctaagggta ttgttgaacg tattggtttt
    24721 accgatgcta ttcttactca caagcctgag ggtaaggata ggtacgaggt ggttaccagc
    24781 atccccgatc acacaaccgg tattaatctt attcttgagg cgcttgttaa cgaaaaacat
    24841 ggcgtaatta agagtttaaa tgagattact gctgttggac accgtgtggc tcatggtggt
    24901 gaatttttca ccaagagtag cttaattgac gatttcacca agagtgaaat tgaaaagtgt
    24961 atagagcttg ccccccttca caatcctgcc aaccttaaag ggattctttc catggaaaag
    25021 ctactcccgg gcgttcccca ggtagctgta ttcgatacat cgttccatca aaccatgcct
    25081 aaatatgctt acctctacgc tattcctcat gactactacg ataagtataa aatccgtagg
    25141 tatggctttc acggaacaag ccataagtat gtagcccaaa aggcatgtcg catacttggt
    25201 gttgatttcg agagccagaa aatcattacc tgtcacctag gtaatggcgc atcaatagct
    25261 gcaataaagc atggtaaatc agttgatacc tcaatgggtt ttactccagt agaaggtcta
    25321 ataatgggaa cccgcagcgg tgatgttgat gctggtattt tactttacct tgctgaaaag
    25381 gagaacttaa gcattaaaga ggttaatgac gtaattaaca agaagagtgg ggtgcttggt
    25441 atatcgggtt tatcgtccga tatgagagac ctgaaaaatg ccgctgctca gggcaatgag
    25501 cgtgctcagc ttgcactcga tatgtactac taccgcgtta aaaagtatgt tggagcttac
    25561 gctgctgcaa tggatggtgt tgaccttatc attttcaccg gtggtatagg cgagaacgat
    25621 ccaattctcc gcgaaaaggt ttcaaccgct atcgatttca tgggtatcga ttttgattct
    25681 gaagccaata aaggtgtgcg cggtaaggat aagttgctaa ccaaacctag ttcaaaggtt
    25741 aaagtgatgg taatcaccac caacgaggag cttgtgattg ccaccgatac ggcaaacatt
    25801 gtaaaagaac ttaaaaagta gtataaacac aaaatgcgat aaagccatga gagtagaaaa
    25861 acttgatcag ctattcgata ttcttcgtag caaaccccgt aaacgattag tagcagctta
    25921 tgctaacgac catcacacca ttgaggctgt tagcatggct attgacatgg gtattgttga
    25981 tgccactctg gtgggcgatg aggcaaccat caagaaagtt tgcggcgaac acaatatcga
    26041 tttttcaaag ttccgcgttg ttcaggaagc cgatgagatg aaagcagccc gcaaagctgt
    26101 tgagcttatt aataagggtg aaggcgatgt actgatgaaa ggtctcgtta gcaccgataa
    26161 gtacatgcgt gccattttgg acaaggagaa tggcttacta cctcctaagg cggtgctaag
    26221 ccatgttacc gttgttcagg tgcccacata ccacaaactt cttatagttg gcgacgtggc
    26281 aatcattcct gctccagatt tgaaccaaaa gatagccatt accaattacc tcatccaaac
    26341 cgcccattca cttggcattg aaatgccaaa ggtagccctc aatgctgcaa ctgagcaaat
    26401 gtcggcaggt atgcaggcat gtgttgatgc agccattatc tcaaaaatgg ccgatagggg
    26461 tcagataaag ggtgccattg ttgatggacc tcttgccctt gatgtggcaa tcgataagga
    26521 atcggctcag attaaaaagg tttcaggtga ggttgccggt gatgccgact gcatactttt
    26581 cccaaatatt gaatcgggta atgtattctt taaggcctgc acaaagcttg ctaagggcga
    26641 acttggagcc atggttatgg gcgcaaaagt gccttgcgtg ctaacatcac gtggcgacag
    26701 cgtacaatca aaaatgtatt caatcgccct tgctgccaac gctgcaaaat agtacaaacc
    26761 agtaacctga aaatattata attagcatgg aagaatataa aatactggct atcaaccccg
    26821 gttccacgtc aaccaaaatt gccgtattcc ggaataataa aaacattttc ctgaaaaaca
    26881 tcaaacactc ggccgacgat ttggcaaagt ttaagaccat tgccgaccag ttcagtttcc
    26941 gtaaggaaat tattcttgag gagttgaaac aggctggtat tgatgttaac aaaatatcgg
    27001 cggtagttgg tcgtggtgga ctgctaaaac ccattgaatc gggggtttac gaggttaatg
    27061 agcaaatgaa agttgactta cgcaacagca taatgggcga gcatgccagt aaccttggcg
    27121 ctttaattgc cgacgatatt gcccgttcgt taccctcggc taaggcatac attgccgacc
    27181 cggtggtggt tgacgagatg gaggatgttg ctcgcatttc aggccatccc cgtttcaaaa
    27241 gggtatctat cttccatgct ctaaaccaga aggccattgc ccgtttgcat gcaaaaagta
    27301 tcgataaaaa gtacgaggag ctcaaccttg ttatcgcaca ccttggggga ggtatatcgg
    27361 tgggtgcaca tcgtcagggc agggtaattg atgtaaacaa cgccctcgat ggcgatggac
    27421 ctttctcacc tgagcgtgct ggaactgtac ctgctggtca gcttgcaaag ctttgcttta
    27481 gcggtgaggt aacctacgat gaggtaaaaa agatgcttac cggtaaggga ggattggttg
    27541 cccatgctgg aaccaacgat gcatatgaaa ttgagctaaa agctcgtgcc ggcgatgcta
    27601 aggctaaact ccttcaggat gctatggcct ataacattgg taaagctatt ggttccatgg
    27661 ctgcagtgct gaaaggtaag gttgatgcaa ttattcttac cggtggtatt gcacacaatc
    27721 ctgacctggt tgattacgtt aaggagatgg ttgggtttat tgcaccagtt gctgtttacc
    27781 ccggtgaaga tgagatgcaa gcccttgcca tgaatgggct catggtgcta aaaggcgagg
    27841 taatgccaaa ggagtataag taatatgaat gattaacaaa gaggaggggc aaatgcccct
    27901 ctt
//
LOCUS       DAOSAL010000029        26239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_1346_length_26239_cov_50.371792, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000029 DAOSAL010000000
VERSION     DAOSAL010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 26239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26239
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1346_length_26239_cov_50.371792"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PK049_05780"
     CDS             <1..308
                     /locus_tag="PK049_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018361219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOK61101.1"
                     /translation="LCDKTSPYALTGAIFSSDRQALNKACRVLRYSAGNFYFNDKPTG
                     AVVGQQPFGGARASGTNDKAGGPFNLIRWTSPRTIKENLNPPIDYRYPYMTNARCGE"
     gene            411..2555
                     /locus_tag="PK049_05785"
     CDS             411..2555
                     /locus_tag="PK049_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011107669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HOK61102.1"
                     /translation="MEIASKTKHVELIAAELNINSRGVANTIKLLDEGATIPFISRYR
                     KEMTGNLDETQITAIRDLSVKYVELDKRKATILSTIEEQGKLTDELRSRIISCYNANE
                     LEDIYLPYKPKRRTKATIAKEKGLEPLALLIFKQQERNIHAKAEAFINDKVADADEAL
                     AGARDIIAEWISEDEKARNIARKHFRKSAIIKSKVVKGKETEGIKYSDYFNWEEDAKR
                     CASHRFLAMQRGQDEGFLRVSITPPAEPVIEELEHVFIRSNGEVADQMKQAIADSYKR
                     LIEPSIENEFTATLKEKADDEAIKVFAENLRQLLLSPPLGQKSVLAIDPGYRTGCKVV
                     CLDPQGNLLHNETIFPHPPQSEQGKAMSKISALVDTYKVEAIAIGNGTASRETEALIK
                     RIRFNRDVKVFVVSEDGASVYSASPVAREEFPEYDVTVRGAVSIGRRLMDPLAELVKI
                     DPKSIGVGQYQHDVDQAKLKGSLDQVVESCVNHVGVNLNTASKHLLTYVSGLGPQLAK
                     NIVEYRAQNGAFKSRDELKRVPRLGEKAFEQCAGFLRIAEAENPLDNTAVHPESYHIV
                     EKMAADLGCSVVDLINNEELRTKINLEKYVTNKVGLPTLNDIMQELSKPGRDPRKAIK
                     VFEFADGIFSIDDLKPGMVLPGIVTNITNFGVFVDIGVKQDGLVHISQLANRYVSNPL
                     DVVKLHQHVKVKVLEVDIPRKRIQLSMKDVEQ"
     gene            complement(3050..4180)
                     /locus_tag="PK049_05790"
     CDS             complement(3050..4180)
                     /locus_tag="PK049_05790"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOK61103.1"
                     /translation="MDFIISLLISTLVSGVAAAILLSRVYKNSIFVKVGIVWLINLLF
                     IMTTVSIRIKFYDGDTLIRYVMLAVNVLFSVLCFYYATIKVVRPLSRAIDKLYKLAEG
                     DFNVPDDDTNVNPKTDLGQLIQATSMIKDNFGRVVLQIQNNVSRLRDMSDELNTISSQ
                     LAEGAAEESSSIEEISSSMEQIAAHIEKNADNSKQTEVYSRKVLDGVQNLNKSAEENL
                     EAINHIAQKINVITDIAFQTNILALNAAVEAARAGAHGKGFAVVAAEVRKLAETSKAA
                     ADEIVQMAHKSVSVAQHTQTMLNELIPDITKTTELIQEITAASIEQHVGVEQVNNAIQ
                     QQNVVAQQNVAISEEVVTTSNKLSEFANQFVELVSYFKIRKK"
     gene            complement(4748..4990)
                     /locus_tag="PK049_05795"
     CDS             complement(4748..4990)
                     /locus_tag="PK049_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61104.1"
                     /translation="MGAPWVELLRDTIRHAYGVTHVVSLTKPTAMFHPRPIAGSGFYA
                     SACFFRSIPSQGKGLGRGLFPQTTINALSPVGAKYL"
     gene            5433..7910
                     /locus_tag="PK049_05800"
     CDS             5433..7910
                     /locus_tag="PK049_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOK61105.1"
                     /translation="MIYPDTFEQKVGFDKVRELTLARCLCELGEQRVRDAGFNTNPDI
                     VFEWIEQTHEMKTICQMEDGFPTDGYIDVTPALRKLNVEGQWLDENELQQLRRSLDTI
                     KQLLAFVKRVSDKYPAIAKLATGINYYPYVIERIDSILDRYGRIKDNASAKLAEIRAS
                     IRAKEASVNKLVQGIMRSAREQGVVEADTQPTVREGRVVIPVAAANKRKLKGIVHDES
                     ATGKTVFIEPVEVVELNNEIRELEYEERREIVRILIELADSIRPYLPELLTAYDFMGE
                     IDFIRAKALLAMEFDGVKPILNSNSTVIYLRQARHPILQLSLKREGKRIVPLDIELNL
                     NDRILLISGPNAGGKSVCLKTVGLLQYMLQCGFLPCVLENSEMGIFSKIFIDIGDEQS
                     LENDLSTYSSHLINMKQMLRHADGQSLVLIDEFGAGTEPTAGGAIAEAILQELCNKGT
                     FGVITTHYSNLKHFAANTPGIVNGAMLFDNERIEPLFKLEIGKPGSSFAFEIARKIGL
                     PEKVLDVASQKVGSDYITFEKHLREISRDKRYWEKKRESIRLANKRAEEAEEKLQKEL
                     EELEKKRKEIIATAKKEAQELLANVNKQIENTIRTIKETNADKERTREARKALENLKI
                     EIENPETANALIERKMELIKQRQERKARKNQQKETITSKPVKEDEVRAIEPGDKVKVE
                     GSDMVGEVISISDKSASVAIGGMITVVRVNKLKRLSTGEYREAAKQKPTLNTGFDVYK
                     RRLNFKSDIDVRGYRAEEALEAVQDLIDDALMLGFNRVRILHGKGNGILKQVIRDFLK
                     ATPGVKNFSDEHVEFGGAGITVVDLDV"
     gene            7970..9289
                     /locus_tag="PK049_05805"
     CDS             7970..9289
                     /locus_tag="PK049_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61106.1"
                     /translation="MELLIYSRQSSTRLEYICRFIFKDILRMPYRLSSSYTEASEYKG
                     PILSYDKQPIPGSISIIPTTLLFETGINQIPMEIGEWNGTPAFPMTWNHKADIPFDLL
                     AGAFYLVTRYEEYLPHKPDKHGRFRFTNSIAYKNGFLEIPVVDVWAHKLGAVIKEKFG
                     NSHSKHRSFTSQITFDLDSAFAFKGKGIIRSALGFTKSAITFDFKKAKTRFRVLANKE
                     QDPFDIYDHLFAKLPAGEATKWFIHVGNWSKYDKQVKTSNPSFLNLVNKLSTRYPIGI
                     HPSYRTFLSDTIFRQELSKLVDILGQAVSASRFHYLRQSIPYSYYPLMRAGITQEYSM
                     GYADKPGFRAGTCTPYPFFDLFDNTTQNLKIFPFAIMDKSLLNIVRYDPNQAFELVKR
                     LVQVVKNVNGNFVSVWHIDYLAQNNPGISLMDILEKTIEMCRNEEKS"
     gene            9273..10214
                     /locus_tag="PK049_05810"
     CDS             9273..10214
                     /locus_tag="PK049_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61107.1"
                     /translation="MKKNLEIEFLHREEIDCKRWNATIDNAFNGSIYAYSWYLDVVSP
                     NWGALATNDYSYLFPLPIKRKYGINYIVQPLHCQQLGLFSTNHISADIVDLFIDQIPA
                     KFKYVDLNLNIHNKTNKHTGHINKRKTYMLDLLNYNEQVFRNYNQNTKRNIFKAIANE
                     FTLTTTLNPVDFMKFYRQYGFMVNRKKDVIVIEKLIESLLTHNKATIAGVTDRNNKLC
                     AVALFAESHNTLHYLLGSAEPENKKKGVMFYLMDGVIKNHCGMDKVLDFEGSMILSIA
                     RFFSGFGAKPCTYFNYHRNSLPAGEALVSIKQKLKLI"
     gene            10219..11307
                     /locus_tag="PK049_05815"
     CDS             10219..11307
                     /locus_tag="PK049_05815"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOK61108.1"
                     /translation="MLSISKISLRELKLFNDDTGLSEKDFLPISKVRAMSLMANPRAN
                     SNDNVLYQAHWNEKLIAYLTVLPDIAFANNQSIPFAWISGAWVHPSFRRQGIATKLID
                     AVVLDWKGMLMATNFSKITEAVFEKTKTFKTVKKIEGQRFFLRKTLLSSYNCCSHSNP
                     ITYITEKFINLLNHSNLTRKFLKIPKNIEIEYFTRPDDEILNKLTLATANTLTKRSTE
                     EINWIIRYPWLINGLLPDRAAQKFFFASVIPAFAYYMVKVFKDDELIGVLLMQHSNTR
                     FTVPYYWFENGTEDVMAKVILLHASRAKASFINIYNQKIVNSMLNLRPYYFYSKKRTR
                     KYLVADSMADSIGDSFELMDGDGDCAFI"
     gene            complement(11313..12020)
                     /locus_tag="PK049_05820"
     CDS             complement(11313..12020)
                     /locus_tag="PK049_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOK61109.1"
                     /translation="MDNFTVDIRSANIFQEDNLVLSDVNFSLGKGEFVYLVGRVGSGK
                     TSLIKTITAEIPLKEGDGLVCGFNLKKLKRKHVPKLRRKIGVVFQDFQLLTDRSVYDN
                     LLFVLKATGWSDKSAIKQRIAEVLDMVELKFKDYKMPHQLSGGEQQRVVIARALLNNP
                     ELILADEPTGNLDMETSEGIMQILHNLSASGISIIMATHNNQIIKKFPARTIKCEKGK
                     VVETEQMTEIDFESLMD"
     gene            complement(12069..12998)
                     /locus_tag="PK049_05825"
     CDS             complement(12069..12998)
                     /locus_tag="PK049_05825"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HOK61110.1"
                     /translation="MEKGIDNKVAVIAGGTGLIGRSLVPMLLNEGYRVKLLTRNPKKV
                     QKSGINVDIVEWSGKPDSELTVLLEGADVVINLSGYSIATLWTKRNRKRIVDSRILPT
                     RALAQAIASCRKTPKVFVQASAVGVYPYSSNSVLTEKSAVGNGFLPALVSDWENEALA
                     CAYVTRVILLRTGIVLASDGGYFPKIAKPIRYFAGAILGDGKNIVPWIHIADHVRAIK
                     FLIDNSAASGAFNLTAPTTNSNADIVFQVAHHYRRPILFRIPSIILHLLPGNMANEVL
                     LANQPVYPERLTQLGFSWNLPVLENAIADLLAK"
     gene            complement(12998..13723)
                     /locus_tag="PK049_05830"
     CDS             complement(12998..13723)
                     /locus_tag="PK049_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61111.1"
                     /translation="MRTYTILVSVILGLLVLQGCKPTQQSQSETITQELTTSPFSNGV
                     PAQVVVIGGQEYKYPIIAIWVEDLRGNFIGTVYASQSISTGIFRYGVYDKGKWLPGER
                     NRPAALPRWKNLTASNAPRVNQGVDAFSGATPSGSFALKTMLPAGIDEFKVYMEVNKP
                     WDFNNYWHNNRYPGDEEYATSGQPAIIYEALVSRKTGDSLFYFKPIGHSNPSGANGEI
                     IPDIATFTSALKIVERAWIELSK"
     gene            complement(13736..16141)
                     /locus_tag="PK049_05835"
     CDS             complement(13736..16141)
                     /locus_tag="PK049_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOK61112.1"
                     /translation="MLRKTLLLFISIVLLNPLNAQDKGMIKGRVFDSKTNEPIPFATV
                     IVYGKIIGTTSDFDGNFIIAGLEPGWVELQVTAIGFKPYISEAVMVTRSKNIFIDIPL
                     EEMPYEIESVVVKASPFRRSEEAPLSLRRIGIDEIERNPGGNRDISRVIQSLPGVAPS
                     LAYRNDVIVRGGGPNENRFYLDGVEIPNLNHFATQGASGGPVGIINVDFVREVNFYSG
                     AFPASKGNALSSVLDFRQVDGNSERLKVKGAVGASDLALTLDGPLSNNTTMIFSARRS
                     YLQFLFNALGLPFLPNYNDFQFKTKTRIDAKNEITVIGLGAIDEFELNLGLKNPDEYQ
                     RYILGYLPVNTQWNYTLGTVYKHYASNGYHTLVVSRNMLNNRQYKFTNNDDNLPKVLD
                     YTSWEAENKLRYERDLGLKNYSINFGAGLEYARYFNSTYRAKFVGETFTPDTYETNMD
                     LFKWNIFGQVNRTFFTKLTVSLGVRADASSYSAEMNNLFKQLSPRFSASYMLKPNLFL
                     NANVGRYYQLPPYTALGFANTTGSLVNKTNGLSYIQSDHLMAGFEWQPNEQSQITVEG
                     FLKFYDRYPVSINDSVSISSKSADYGTFGDEPLVSTGKGRTFGVELLYRNKKLLGFNT
                     LFSYTLVRSETSLMNSNLKPSGKWIPTSWDNRHLVTLTATRSFKKGWDFGFKWRFVGG
                     PPYTPYDLATSSLINVWNVQRQPSLDYSSFNSLRLQSFHQLDIRVDKSYYFQSWMLNF
                     YVDIQNVYNFQGDRPPLYTTVSDTNGNPVVDPNDATRYLLKKIEGTGGGTVLPTIGVI
                     VEF"
     gene            complement(16248..18290)
                     /locus_tag="PK049_05840"
     CDS             complement(16248..18290)
                     /locus_tag="PK049_05840"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HOK61113.1"
                     /translation="MTDKTGFDSFVEAILWIVKNLAVGVLILFISRLIFLFSFANWND
                     LSGLSGDIAKAFTVGLKFDLKVLTIALLPCLLLAFVRLIFSDRVGNFTKFFRYYSTAL
                     MILVVMFEVINFYFYKFYHTKISVIIFGFFEDDTWAVLVSIWKEYPVIPILALFIVLI
                     WTFSKLFVHLYKPISCNFCKRLRPIWLRVLIVVAISGLYFLGMLGNIGVTSIDEQHTI
                     ISKNIFVNTLPVNGLFSTKIAWTDRQRSRIDINIPKMLKSLKFKSIEDAAGVFYGLKV
                     DSVSAETFYTTTPQNHFLENNPPHVVFILMESMSNFYIDMHSDSCNMLGSLADVLDSC
                     YLFRNFLPSYSGTIYSLESILVNTPKSPLSQSPYQNFSFDASAAKPFYEKGYTNIFLT
                     GGKMGWRNMDKFIPRQYFNSIEAEPTLRKLYPEGKEGEWGMHDEVLFQRAFDVLANSN
                     GKPYLIFGMTISHHTPYDIPDNYQGYPISIPDWARKRMKFDEAIVLKGLRAYQYANSC
                     LGDFIKRIITSPLGEKTIIVATGDHNIRQNFEYPQADLFMQYSVPLLIYIPPKYRPKN
                     PINTRRFASHKDIFSTLYNLSLSSARYPNFGNNLCNTYCANDFGLYCYSIAADSIGLV
                     DFSSTPLFYRWSDREHRKLEPSNYSPDKHLDSLMLRAKALTACMNYLLIKDIEKNR"
     gene            complement(18292..19083)
                     /locus_tag="PK049_05845"
     CDS             complement(18292..19083)
                     /locus_tag="PK049_05845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HOK61114.1"
                     /translation="MVRLDIYLVKKGLCRSRERAKAHITAGEVLVNGKKVTKPSFAVA
                     ETDSIQLSINPNDFFSRGELKLQKAIDDFGLNFQDKLVLDVGASTGGFTYCALKHGAR
                     FVWAVDVGTNQLDPELINNSRVCSLEKTDIRTLDISALGTRVDVITADLSFISLTQVA
                     QSLVKFLKPDGFMVLLIKPQFEAGKENIGKNGVVKDKKVHEVVIENVAKCFAQHNIYL
                     NSITFAPLKGKGYNIEYLALFSNSIKGLPNVKTIVDSAFENNRLV"
     gene            complement(19070..19639)
                     /locus_tag="PK049_05850"
     CDS             complement(19070..19639)
                     /locus_tag="PK049_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61115.1"
                     /translation="MEIDNLTERLKYSLRKSEVQQLASFFSAIPNGTDTLFELSLSKN
                     SQLAFHAAWVLENCLLDNTSLLDVHADRLLAVIPSLQNNSVRRHFCKLFKTWLESNQN
                     KLTEWIEARNSKVEGLIEACYGWLLSTDVPVAVRVHCLEIMWILSNQYTWIAEELPNT
                     LRLIQIDSTQGVKAMVRRIKKTRAGNGKA"
     gene            19672..20379
                     /locus_tag="PK049_05855"
     CDS             19672..20379
                     /locus_tag="PK049_05855"
                     /inference="COORDINATES: protein motif:HMM:NF015949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HOK61116.1"
                     /translation="MANNYDILEQLFDKAKSINPFSDGHFTFGEKYVAVQNGLGQIGV
                     CATLGKKVDHTNLENFDLGKAECRVAANAFANATLNYLVEPDGVGDIFDTVDFSSHTN
                     LVMIGYFGSLVQKLQTKGIKPLVFDIDQHEAPVLPMDKQHEYLTVADCVILTSTSLSN
                     GTFNGIVKTVNPQCSIYMLGPSTPLDDLMFTIPQVKGLFGSIFPIHSSETLNLIAQGY
                     GTKGFMHTMRKVYRIRK"
     gene            complement(20448..22607)
                     /locus_tag="PK049_05860"
     CDS             complement(20448..22607)
                     /locus_tag="PK049_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOK61117.1"
                     /translation="MRKSFLLILLAVSLTFRGMADEGMWIPMLIGKNIAEMQRMGFKL
                     TAEDIYSVNKASLKDAIVHFGGGCTGELISAEGLLITNHHCGYPEIQSHSTVENNLLA
                     NGFWASSKAEELPNPGLKVRFLVRMDDVTNLVLKNIAPSMTEQERAKSIQQVADSLIA
                     KAEKEGIGYEASIRPFYYGNQYYMFVYQVFEDVRLVGTPPESIGKFGGDTDNWVWPRH
                     TGDFSLFRIYADKNNNPAPYSPSNVPYKPKKFFKISMKGVNEGDFTLVYGFPGRTQQF
                     ITSHAVRLLIDQSNPHKINLRDIRLSIFNKFTESNDTIAIKYAAKHARVANAWKKWIG
                     ETMGLRRLDAVSKKQEQESMFAAWAATNPELKERYGWLLPTFDRLYAELAPLSLVVDY
                     RNEAVYGVEMLSFASRFERLLSMALDPGVDKGKLAEQRNKLVEYVRDFFKDFELSVDR
                     EVFAAMMQAYYERIPQQLQPEFLGKFIQQSGNWRELAKQIYSQTAFADSVRVLTLLND
                     IDSTNAREFLNDPIFSIYFEFEDLYRKNVQKRYFEITDSLDILYRTYVEGLMRMQPDK
                     RFFPDANSTLRIAYGKVGGFAPRDGVVYDYYTTIDGIYEKSQQRDVPDYVPPKRLIEL
                     YQTKDFGPYAVNGTVPVAFIASNHTTGGNSGSPVLDAEGNLIGVNFDRCWESTMSDVM
                     FDPNYCRNISLDIRYALFVIDKYAGAKNLINEMVILQ"
     gene            complement(22624..23232)
                     /locus_tag="PK049_05865"
     CDS             complement(22624..23232)
                     /locus_tag="PK049_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HOK61118.1"
                     /translation="MLLHELLQGKRLVLASKSPRRRELLKGLDVEFEIWDTNHDDEEP
                     FLANLPLADVPVYLAKQKATHLADRLDDSTILITCDTIVICCNQILGKPKDEADARRI
                     LSALSNKSHTVITGVCLKSKNSERVFDVATEVHFKRLSMEEIDYYISRYKPYDKAGAY
                     GVQEWIGYVGVERIDGSYFNVMGLPIHTLHSQLISFINSLSK"
     gene            complement(23245..24207)
                     /locus_tag="PK049_05870"
     CDS             complement(23245..24207)
                     /locus_tag="PK049_05870"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HOK61119.1"
                     /translation="MLKYFRLVRFKNLLIIAATMVLVRYMLIQTILSGYGLELQMPIW
                     YFVALVLATMLVAAGGYVINDYFDTKADLINRPETVVVGKQVTRRGAIAFHLVLSILG
                     IFLGTVVSFRVGRPVFSLLFFLTAGVLWFYSTTYKRQLFVGNLVVAFLTAMVPLIPLI
                     FEFPLLGTFSDVIFIYQLNMKVIVYWVGGYAFFAFMLTLAREIIKDIEDFEGDTSLGR
                     NTIPVHFGVKTSKLIIATVLSLTLIALLVAFSAYLFKLSVKPFDYVSLVYLVIFIVIP
                     IFILIFSVLLASSKKDYHKASSLCKIIMLLGLIYTLIFRFIVNL"
     gene            complement(24222..24752)
                     /locus_tag="PK049_05875"
     CDS             complement(24222..24752)
                     /locus_tag="PK049_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HOK61120.1"
                     /translation="MVGKKNFKELLSHVKAFAFDVDGVFTDGTIIIHPSGELLRTSNT
                     RDGYAVHVAIERGFPVAIISGGKSEAVRQRFQGLGVTDIYLGTTDKIEALEDFRFKYG
                     LELSDILYMGDDLPDFEVLQRVGIPTCPRDAAPEIQQISAYISSFAGGKGCVRDVIEQ
                     VLRVNGKWGPSANGLQ"
     gene            complement(24768..25565)
                     /locus_tag="PK049_05880"
     CDS             complement(24768..25565)
                     /locus_tag="PK049_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018668359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HOK61121.1"
                     /translation="MIHDVVLLGAGNLATSLAVALSNAGFPILQVYSRTVESASKLAN
                     LVGAEPICKLESLRTSASIYIVALPDDVIASIVSVLPELKGCVVHTSGSTPMSVFEGL
                     RAKGFGVFYPFQTFSRSKIVPFSNIPICLEASNDECYASLEQFASKLSGTIVPMSSEQ
                     RRWLHLSGVFACNFTNHMLALAYQLTTEHHISFEVVKPLVEETIRKAFEGNPALYQTG
                     PAVRNDIQTMERHAQMLKVFGLADLYKELSLSIQKLSGSDKGLKNRT"
BASE COUNT         8064 a         5682 c         5632 g         6861 t
ORIGIN      
        1 agctttgcga taaaacctcg ccatatgcac ttacgggtgc aatattctcg tccgataggc
       61 aagcattgaa taaggcatgt agggtactgc gttactcggc tggtaatttc tactttaacg
      121 ataaacccac aggggctgtg gtaggacaac aaccctttgg tggcgctcgt gcaagtggaa
      181 ccaatgataa ggccggtggc cctttcaacc tgattcgctg gacaagcccc cgaactatca
      241 aggaaaacct aaacccacca atcgattaca gataccccta catgactaat gcccgctgtg
      301 gcgaatagca taaattcata aaattaagaa cggctactta aattaaggtg gccgtttttt
      361 tttgcaagcc aaaatattat ttcgtttttt tgcgatgaaa attgaaattt atggagatag
      421 caagtaaaac caagcacgta gaactgatag cagctgaact taacattaac agccgaggag
      481 ttgcgaacac cattaagcta cttgatgagg gtgctacaat ccctttcata agccgctacc
      541 gcaaggagat gaccggaaac cttgatgaaa cacaaattac tgctatccgc gacctatctg
      601 ttaagtatgt tgaacttgac aagcgcaagg caaccatact atccaccatt gaagagcaag
      661 gtaaactaac cgatgaacta agatcaagga ttataagctg ctataacgca aacgaactgg
      721 aagatatcta cctaccatac aaacccaagc gaagaactaa agcaaccata gccaaagaga
      781 aaggattaga accattagcc ttactgattt ttaagcagca ggaaagaaac atccatgcta
      841 aagcagaagc ctttataaac gataaagttg ccgatgctga tgaagcactt gctggcgccc
      901 gcgatattat tgcagaatgg attagcgaag acgaaaaagc gcgaaacata gcccgaaagc
      961 atttccgaaa atcggccatc ataaagtcga aggtggtcaa aggcaaggaa acggagggca
     1021 ttaaatactc cgactacttt aactgggagg aggatgcgaa gcgttgcgca tcgcacaggt
     1081 tcttggccat gcaacgtgga caagacgaag gatttctacg agtaagcata accccaccgg
     1141 cagagcctgt aattgaagag ctggaacacg tatttatccg ttcaaacggt gaagttgccg
     1201 atcagatgaa gcaagcaata gccgacagct acaaaagact aattgaacca tcaattgaaa
     1261 acgaatttac agccacgcta aaagagaaag ctgacgatga ggctataaaa gtttttgccg
     1321 aaaacctgag gcaactctta ctctcgccac cccttgggca aaaaagcgtg cttgccattg
     1381 acccaggcta ccgaacggga tgcaaggtgg tttgtcttga cccgcaaggg aacttactgc
     1441 ataatgaaac catttttcca cacccacccc aaagcgagca agggaaggcc atgagtaaga
     1501 tatcggcatt ggttgacacc tacaaggttg aagcgatagc aattggtaat ggtacagcga
     1561 gccgtgagac cgaagcattg attaaacgaa ttaggtttaa ccgcgatgta aaagtttttg
     1621 ttgttagcga ggatggtgca tcggtttact cggcttcacc ggtggctcgc gaagaattcc
     1681 cggaatacga tgtaaccgtt cggggagcgg tttcaattgg ccgtcgacta atggacccat
     1741 tggctgagct ggttaagata gatccaaagt cgattggcgt aggccaatac cagcacgatg
     1801 ttgaccaagc caaattgaaa ggtagcctcg accaggttgt ggaaagctgc gttaaccacg
     1861 ttggggttaa cctgaatact gccagcaagc acctactaac atacgtttcc ggacttggtc
     1921 cacagctggc aaaaaacatt gttgaatacc gagcccaaaa tggcgcattt aaaagcagag
     1981 atgagctaaa gagggtccct cgcttaggcg aaaaggcatt tgaacagtgc gcgggattcc
     2041 ttaggattgc cgaggctgag aacccactcg ataatacagc cgttcatcct gaaagctacc
     2101 acattgtaga gaaaatggca gccgatttgg gctgctcagt tgttgactta atcaataacg
     2161 aggagctaag gacaaaaatc aaccttgaaa aatatgtaac gaacaaggta ggcttaccca
     2221 cactaaacga catcatgcaa gagctttcaa aacccggacg cgacccccga aaagccataa
     2281 aagtatttga gtttgccgat ggcattttca gcattgatga ccttaaaccg ggtatggttt
     2341 taccgggcat tgtaaccaac atcaccaact ttggggtatt tgttgatata ggagttaagc
     2401 aggatgggct ggttcatata tcgcaattgg ccaaccgcta cgtttcaaac ccgctcgatg
     2461 tggttaagct acaccaacac gtaaaggtga aagtgcttga ggttgatatt cccaggaaaa
     2521 ggattcagct ctcaatgaag gatgtggagc agtaagaaat gcatttacac caagtgacat
     2581 gaattttaag gaatgagccg gaatgagtcg gaatagacag aaagtgatgg agttagagga
     2641 agttagagga agttagagga agttagagga agtttggtct gtgaaacgtg agccgggaaa
     2701 cgtgaaacgt gaaaagaaga attgagaata tgatggagtt agaggaagtt tggcccgtga
     2761 aacgtgagcc gggaaacgtg aaacgtgaaa agaagaattg agaatatgat ggagttagag
     2821 gaagtttggc ccgtgaaacg tgaaccggga aacgtgaacc gtgaaaagaa gaattgagaa
     2881 tatgaggaag ttagaggaag tttggtccgt gaaacgtgag ccgtgaaact tgaaccgtga
     2941 aaagaagaat tgagaatgtg atgaagttag aggaagttaa aggaaaagtg aacagttaaa
     3001 acaaaattct ctaattgaaa tatacagtta atgaatcaac taacaaaaac tatttcttcc
     3061 taatcttaaa atacgatacc aattccacaa actggttagc aaactctgaa agcttattgc
     3121 tggttgtaac aacctcctcg ctgatagcaa cattttgctg tgcaacaaca ttttgctgtt
     3181 gaattgcatt atttacctgc tcaacgccca catgttgctc aatgcttgca gcggtaatct
     3241 cctgaattaa ctcggtggtt ttagtaatat cggggataag ttcgttgagc atggtttggg
     3301 tatgctgagc cacgctcacg ctcttgtggg ccatttgaac aatttcatcg gctgcggcct
     3361 tactagtttc agcaagttta cgtacctcag cagcaaccac ggcaaagccc ttcccatgcg
     3421 caccggcacg ggctgcctca acggcggcat taagtgcaag tatattggtt tggaaggcaa
     3481 tatcagttat aacattgatt ttttgggcaa tatggttaat agcctccaaa ttctcctcag
     3541 ccgatttatt caggttttgc acaccatcta atactttacg cgagtaaact tcggtttgtt
     3601 tggaattatc ggcattcttt tcgatatgag ctgcaatttg ttccatggag gaagaaatct
     3661 cctcgattga cgaagattcc tcagcagccc cttcagccaa ttggcttgaa atagtgttta
     3721 gctcatcgct catgtcgcgt aacctgctca cattgttttg gatttgcaaa acaactcgtc
     3781 caaagttatc ctttatcata ctggtagcct gaataagctg acccaaatcg gttttggggt
     3841 taacatttgt atcgtcatcg ggaacgttaa aatcgccctc ggctagcttg taaagcttat
     3901 caattgccct tgatagcgga cgaaccactt ttatggttgc gtaataaaag catagcacgc
     3961 tgaaaagcac gttaacggca agcatcacgt aacgaatcaa tgtgtcgcca tcgtaaaatt
     4021 ttatcctgat gctcaccgtt gtcatgatga acagcaggtt tattagccaa acaatgccaa
     4081 cttttacaaa tatactattc ttataaactc tgctaaggag aattgcagcg gccactcccg
     4141 aaactagagt gctgataagt aaactaatta tgaagtccat agatagtttt ttaaaatgtt
     4201 ggctactgat ttgaactaat tgatgtgtaa gttataaaaa aaatcaatat caaattacag
     4261 cattaacata atggtatccg aaacatgaat agctcaagga ttacaatcgc aattgcattc
     4321 taaaccataa caattcaggt tttgacaact gaaacctata tcctttcaat acattaacct
     4381 taaatagaat gcaatgttgt atagtaaagg ttatatgtca tgttgcgcga aagcgaaata
     4441 ttacgctgtc gtgctgagtc ccgatttata gggatagact tcgccaaagc atctcattgc
     4501 tcattttctt caatcctttg aatagatccc tcgactttgc tagggatgac aaaaacccta
     4561 tcttcctttc tgctcccttg ttatgctgag ctagccgaag tatctctttg ccttaaaccc
     4621 tctaatactt gttaattttt tggaaactgc tctttgcggt gcttttttat tgcgatgcaa
     4681 agcttcacct ttgctctttg gaaaaagtat tgcgtgcatc gctatacatg taggaataat
     4741 cttggtgtta caaatatttc gcccctacgg ggctgagagc gttgattgtt gtttggggga
     4801 aaagacctct ccccagccct tttccttgag aggggatgga tcggaagaag caagcagagg
     4861 cataaaagcc tgacccggca atgggtcggg ggtggaacat ggcggtgggt ttagtaagcg
     4921 ataccacatg agtcacgccg taggcgtggc gaatagtatc gcgtagcagt tccacccacg
     4981 gggcacccat cgggtcaggc ttttcagcct tgattttctt tggttctttc ttgtatcaag
     5041 acaagaaaga actttaaaat agctctttgc aatgtttttc ctttgcgatg caaagcttca
     5101 cccttgctct ttggaaaatg tattgcgtgc atcgcaatac aagtgggagt atacataggt
     5161 ttacaaacgt ttagccccta cggggctaga agcggtgttc gttgttcgtg aatcgttgtt
     5221 cgtgaagcta ttgtcatgtt gagcacaagt gaaacgtctc atggggtcga ttagagatca
     5281 ttcgctgcgt gcaagatgtc agacatgtat atttttgtta actaaacaac attgaaatag
     5341 aatgagttat tttctatact acaagcaaca aattttcatc cacatcggta tcaaatcgct
     5401 aactttgcac aaactaacat tttgcattta caatgattta ccccgatacc tttgagcaaa
     5461 aggttggatt tgataaggtt cgcgagttaa cgcttgcgcg ctgtctatgt gaacttggcg
     5521 aacagcgagt aagggatgcc ggattcaaca ccaatcctga cattgttttt gaatggattg
     5581 agcaaaccca tgaaatgaag actatctgcc agatggagga tggcttcccc accgatggct
     5641 atattgatgt tactccagcc ctacgtaaac ttaatgtgga agggcaatgg ctcgatgaga
     5701 atgagctaca acagctacgg cgctcgttgg ataccataaa gcaacttctt gcatttgtga
     5761 aaagggtatc ggataaatac ccagcaattg cgaagcttgc aacaggaata aactactacc
     5821 cttatgttat tgaacgtatt gacagtattc tggacagata cggtcgaata aaggataatg
     5881 catcggcaaa gctggccgaa attagagcaa gcataagagc aaaggaagca tcggtaaaca
     5941 agctagtgca aggaataatg cgcagcgctc gcgaacaagg agttgttgaa gccgacacac
     6001 agccaacagt ccgcgaagga cgggtggtta ttcccgttgc ggctgcaaac aaaaggaaac
     6061 taaaggggat tgttcatgac gagtcggcta ccggcaaaac cgttttcatt gagcccgttg
     6121 aagtggttga acttaacaat gaaattcggg aacttgagta cgaggagagg cgcgaaatag
     6181 ttagaatact gattgaacta gccgatagta taagaccgta cctgccggag ctgcttactg
     6241 cctacgattt catgggcgaa attgatttca tcagggcaaa agcgctacta gccatggagt
     6301 ttgatggggt caaacccata ctaaacagta actcaacggt aatatacctt cgtcaagccc
     6361 gacatccaat tctacagctg agccttaaaa gagaggggaa aaggattgtt ccgctcgata
     6421 ttgagctgaa cctaaacgat aggattctac tgatatcggg ccccaatgcc gggggcaaat
     6481 cggtttgttt aaaaacggtt gggttactgc agtacatgct acaatgcggt ttccttccgt
     6541 gtgttttgga aaattccgaa atgggaatat tttcaaagat tttcatcgac attggcgatg
     6601 agcagtcgtt ggagaatgac ctaagcactt acagctcgca cctaatcaac atgaaacaaa
     6661 tgcttaggca tgccgatggc caaagccttg tgctaattga tgagtttggg gctggaactg
     6721 aacctaccgc tggaggagca atagccgagg caatccttca agagctatgc aacaaaggaa
     6781 cttttggggt tattacaact cactactcca accttaaaca ttttgccgcc aatacacctg
     6841 gcattgtaaa cggagccatg ctttttgaca acgaacgtat tgagcccctg tttaaacttg
     6901 aaattggcaa gcccggcagc agctttgcat ttgaaattgc gagaaaaatt ggacttccgg
     6961 aaaaggtttt ggatgtggca tcacagaagg tgggctccga ttacatcacc tttgaaaagc
     7021 atctgcgtga gatatcgcgc gacaaacgat actgggaaaa gaaacgcgaa agcataaggc
     7081 ttgccaataa acgagccgaa gaagctgagg aaaaactcca aaaggaactg gaagagcttg
     7141 agaaaaaacg taaggagatt attgctactg caaaaaagga agcgcaggaa ttgctggcca
     7201 atgtgaataa acaaattgaa aacaccataa gaaccattaa ggaaaccaac gccgataagg
     7261 aacgaacgcg cgaggccaga aaggcgttag aaaatttaaa aatcgaaata gaaaatccag
     7321 aaaccgctaa cgctctaatt gagcgtaaaa tggaactcat taagcaacgg caggaaagaa
     7381 aagcacgaaa gaatcagcaa aaagagacca taacaagcaa acctgtaaag gaggatgaag
     7441 taagagcaat agaacctggc gataaggtga aagtggaagg aagcgatatg gttggcgagg
     7501 tgattagcat tagcgataaa agcgcatcgg ttgccatagg tggaatgata accgtagtta
     7561 gggttaataa gcttaagaga ttatctactg gcgaataccg tgaagcggca aagcagaaac
     7621 caactttaaa cactggattc gacgtttata agcggaggtt aaacttcaag tccgatattg
     7681 atgtacgagg atacagggcc gaagaggccc ttgaagcagt tcaggattta atagatgatg
     7741 ccctgatgct cgggttcaac cgggtgcgga ttctgcatgg gaaaggaaat ggaattctca
     7801 agcaggtaat tcgtgacttt ttaaaggcta cgccgggcgt aaaaaacttt agcgacgaac
     7861 acgtggagtt tggcggggca ggaattacgg tggttgacct tgacgtttag cgccttagcc
     7921 tatgaaaatt gaaaggcatt ttgtatattg ccaatcaaaa taacaggttg tggaattact
     7981 tatttacagc cgtcaatcaa gtacgaggct agaatatatt tgccgattta ttttcaagga
     8041 catactccgt atgccctacc gattatcgag ttcctacacc gaagcatcgg aatacaaagg
     8101 gccaatacta agctacgata aacagccgat acccggctca ataagtatta taccaacaac
     8161 cttactgttt gaaacaggaa tcaaccaaat tccaatggaa ataggtgaat ggaatggcac
     8221 gcctgcattc cccatgacct ggaaccataa agccgatatt ccttttgacc tgcttgcagg
     8281 cgcattctac ctggttaccc gctacgaaga gtacttaccc cacaagcccg ataaacacgg
     8341 acgtttcagg tttactaata gcattgcata caaaaacggt tttctggaaa tacctgttgt
     8401 tgatgtttgg gcacacaagc taggagcagt tattaaggaa aagtttggca actcacactc
     8461 aaaacaccgt agctttacca gccaaataac ctttgacctg gacagcgcat ttgctttcaa
     8521 gggcaaagga ataatcagga gcgctctggg ctttaccaaa tcagccatta cattcgattt
     8581 taaaaaggct aaaactcgat tcagggtgct tgcaaacaaa gaacaagacc cctttgatat
     8641 ttacgaccat ttatttgcta aactaccagc aggcgaagcc accaaatggt ttatacatgt
     8701 tggcaactgg agcaaatacg ataagcaggt aaaaaccagt aacccttcgt tcttaaatct
     8761 ggtcaacaag ctgtcgacac gctatccaat tggtatacac ccatcataca gaacctttct
     8821 gagcgataca attttccgac aggaactgtc gaaactagta gatattctag gacaagctgt
     8881 atcggcatcg aggtttcatt atctacgaca aagcatacct tactcatact accccctaat
     8941 gcgagcagga atcacccagg agtactcaat ggggtatgcc gataagccag gatttagggc
     9001 aggaacctgt accccctacc cttttttcga cctatttgat aacacaaccc agaatctaaa
     9061 aatcttcccc tttgccataa tggataaatc gctgcttaac attgtccgct acgacccgaa
     9121 tcaagccttt gagctagtta agcgcctggt acaggttgtt aaaaatgtaa acgggaactt
     9181 tgtcagcgta tggcacatcg attacttagc tcaaaataac ccaggtattt cgctcatgga
     9241 tattctggaa aaaaccattg aaatgtgcag gaatgaagaa aaatcttgaa atagagtttt
     9301 tacatcgcga ggagattgac tgtaagcgtt ggaatgcaac aattgacaat gcctttaatg
     9361 gaagtattta tgcatactca tggtatctgg atgtggtttc tccaaactgg ggggctctgg
     9421 caacaaatga ttacagctac ctttttccgc ttccaataaa acgaaaatat ggaataaact
     9481 acatagttca acccttacac tgccagcagt tggggctatt ctcaacaaat cacatttcgg
     9541 cagatattgt agatttattc attgaccaaa tacccgcaaa gttcaagtat gttgatctaa
     9601 atcttaacat ccataataaa accaataagc acacgggtca tatcaacaaa cgaaaaacat
     9661 acatgctcga tttattaaac tataacgagc aagtattcag aaactacaac caaaatacca
     9721 aaaggaatat ttttaaagcc attgccaacg agtttacact taccaccacg ttaaatccgg
     9781 tagattttat gaaattttac aggcaatacg gctttatggt taatcgaaaa aaggatgtca
     9841 ttgttattga gaaactcatt gagagccttc taacccataa taaagcaacc attgccggag
     9901 taactgatag gaataacaaa ctttgtgcag tggcattgtt tgctgaaagt cacaacacct
     9961 tacactacct gcttggctca gccgaacccg agaataagaa aaaaggtgta atgttttact
    10021 taatggatgg agtaataaaa aaccactgcg gaatggataa agtacttgac tttgaaggtt
    10081 caatgatttt atcaattgca cgatttttta gtggttttgg ggcaaaaccg tgtacttatt
    10141 ttaattacca tcgtaactcc ttaccggcag gtgaggcatt ggttagcatc aagcaaaaac
    10201 ttaaactcat atagggatat gctgtcgata tcaaaaatct cgttaaggga gctgaaacta
    10261 ttcaatgatg atacgggttt aagcgagaaa gattttttgc ccattagtaa ggttagagca
    10321 atgtcgttaa tggcaaatcc ccgtgccaac tcaaacgaca atgtacttta ccaagcccac
    10381 tggaacgaaa aacttattgc atacctaact gtattgcccg atattgcatt tgccaacaac
    10441 caatcgatac catttgcatg gattagtgga gcatgggttc atcccagttt ccgaaggcaa
    10501 ggaatagcca caaaactaat tgacgcagtg gtattggact ggaagggaat gttaatggca
    10561 actaacttct caaaaataac cgaagctgta tttgaaaaga ccaaaacatt taaaactgtc
    10621 aaaaagattg aagggcaaag gtttttcctg cgcaaaaccc ttttaagttc ttacaactgc
    10681 tgcagccata gcaatcccat cacctatata accgaaaagt tcataaactt gttgaaccac
    10741 tcaaacctta cacgcaaatt tcttaaaatt ccaaaaaata ttgagattga atactttaca
    10801 agacccgacg acgaaatact aaacaagcta accctagcga cagccaacac gcttacaaaa
    10861 cgaagcactg aagagattaa ttggattatc cgatatccgt ggttgattaa cggccttttg
    10921 cccgacagag cagctcaaaa gttctttttt gcctcggtga tacctgcctt tgcatattac
    10981 atggtaaaag tatttaaaga tgatgaactt ataggtgttt tactcatgca gcatagcaat
    11041 acccgtttta cagttcctta ctactggttt gaaaacggca ccgaagatgt tatggccaag
    11101 gtgatactgc tgcatgcttc aagggccaaa gcatcattta tcaacattta caatcaaaag
    11161 attgtgaatt ccatgcttaa tctacgacca tactacttct acagcaaaaa acgaacccgt
    11221 aaatatctag tggccgacag tatggctgat tcaataggag acagttttga actaatggat
    11281 ggcgatggtg actgtgcatt tatatagtgg cgctaatcca tcaaactttc gaaatcaatt
    11341 tcggtcattt gctcggtctc aacaacctta cccttttcac acttaatggt gcgggctgga
    11401 aatttcttta tgatttgatt gttgtgggtt gccattatta tggagattcc tgaagctgag
    11461 agattatgga gtatttgcat gattccctcc gaagtctcca tatctaggtt accggttggc
    11521 tcatcggcaa gaataagttc aggattattc aatagtgccc ttgcaatcac aacacgctgt
    11581 tgctcaccac ccgatagctg atggggcatc ttataatcct taaactttag ctctaccata
    11641 tccagaacct cagcaatacg ctgcttaatt gctgatttat cgctccatcc ggtggctttt
    11701 agcacaaaaa gcaggttatc gtatacactt ctgtcagtaa gcagctgaaa atcctgaaat
    11761 actacaccaa tttttcggcg taacttagga acatgcttgc gtttaagctt cttcaggttg
    11821 aaaccacaca caagcccatc gccctctttg agaggtattt cggcggtaat ggttttaatt
    11881 agacttgttt ttccgctacc aactctaccc accaagtaaa caaactcacc cttacctagc
    11941 gaaaagttaa catcggacag aacaagatta tcctcctgaa atatgttggc tgagcgaata
    12001 tcaactgtaa aattatccat ataatgccga aatgtttcaa aacgctaagg taagaaaaaa
    12061 aatagaatct actttgccaa caaatcggca atggcatttt caaggacagg gaggttccag
    12121 ctaaagccta actgggttaa acgttctggg taaacaggct ggtttgccaa tagcacctcg
    12181 ttagccatat ttcccggtaa aaggtgcagg ataatagatg gtatgcggaa taggattgga
    12241 cggcggtagt gatgggcaac ctggaaaact atatcggcat tggaattggt agtgggtgcg
    12301 gtaagattaa atgctcccga ggcagccgaa ttatcgataa gaaactttat tgccctaaca
    12361 tggtcggcta tatgaatcca cgggacaata tttttaccat cgcccagtat tgcgcctgca
    12421 aagtaacgga ttggtttcgc aattttagga aaataaccac catcgctggc cagcactata
    12481 cccgtgcgga gtaaaatcac ccgggtaaca taagcgcaag ccaaagcctc attttcccaa
    12541 tcgcttacaa gggccggtaa aaaaccatta cctactgccg atttttcagt taaaacagaa
    12601 ttactactat atggatatac accaacagct gaagcttgga caaacacttt gggtgtttta
    12661 cggcatgatg ctatagcctg cgctagagcc cttgttggaa gtattcggct atcaacaatg
    12721 cgttttctat ttcgttttgt ccaaagcgtt gcaatactgt agcccgaaag gttaataaca
    12781 acatcggcac cctcaagcag gacagttaat tcgctatcgg gtttaccgct ccattctaca
    12841 atatcaacat tgataccaga tttttgaacc tttttggggt tacgggtaag caattttacc
    12901 cgataccctt catttaggag catgggaacc agactccttc ctataagacc agttccccct
    12961 gcaattactg caaccttgtt atcaatacct ttctccatta tttggacagt tcaatccagg
    13021 ctctttctac tatttttaac gcagatgtaa aggtagctat atcgggaata atttcgccat
    13081 tagcccccga agggttgcta tggccaatgg gtttgaaata gaataggcta tcgcccgttt
    13141 ttctagaaac caaagcctca tatatgattg ctggctgccc cgatgtggca tactcctcat
    13201 cgcccgggta ccggttgttg tgccagtagt tgttaaagtc ccacggttta ttgacctcca
    13261 tatacacctt aaattcatca ataccagcag gcagcatggt ctttaaagca aatgaaccgg
    13321 aaggggttgc accgctaaac gcatcgaccc cctgattaac gcgaggagcg tttgacgcgg
    13381 ttaaattttt ccagcgcggc aatgcagccg gcctatttcg ctcacccggc agccatttcc
    13441 ctttatcgta aaccccatat cggaaaattc ctgttgaaat ggattgcgat gcataaactg
    13501 tgcctataaa gtttcctctt aaatcttcaa cccaaatggc aataatggga tacttgtatt
    13561 cctgtccgcc aattactaca acctgggctg gtacaccatt gctgaatggc gaggttgtca
    13621 gttcttgagt aattgtttcg gactgtgact gctgggttgg tttgcacccc tgcaaaacca
    13681 acaaacccaa aatcacggat actaggatag tatatgttct catcgttatc attttttaga
    13741 attcaacaat tacgccaatg gtaggtaata cggttccccc accggttccc tcaatcttct
    13801 ttagcaggta tcgggtagcg tcgtttgggt caaccacagg attaccattg gtatcggaaa
    13861 ctgtagtgta aagcgggggc ctatcgccct ggaagttgta aacattctga atatcgacat
    13921 agaagttcag catccacgac tgaaagtagt aagacttatc gacacggata tcgagctggt
    13981 ggaacgactg cagtcgaaga ctgttgaaac tggagtaatc gagcgatggc tgacgctgaa
    14041 cattccatac atttatcaac gatgaggttg ctaaatcgta aggggtgtac ggcgggccac
    14101 ctacaaatcg ccacttgaag ccaaaatccc atcctttttt aaaggaacga gtggctgtaa
    14161 gtgtaaccaa atggcgatta tcccaggatg ttggaatcca tttacctgaa ggttttaggt
    14221 tactattcat taagctagtt tcgcttctaa ccaaggtgta tgagaaaaga gtattgaaac
    14281 ccaaaagctt tttatttcgg taaagtaact caaccccaaa tgttcgccct ttccctgtgg
    14341 acactaaagg ctcatcgcca aaggttccat agtcggcact tttgcttgat attgacaccg
    14401 agtcgtttat ggaaaccggg tacctatcgt agaatttcag gaagccttca acagtaatct
    14461 gactttgctc attgggttgc cattcaaaac cagccatcag atggtccgat tggatatacg
    14521 acaaaccatt tgttttattc actaaagagc cagtggtgtt ggcaaacccc aaggctgtgt
    14581 atggaggcag ttggtagtat cttccaacat ttgcattcaa aaataaattg ggtttaagca
    14641 tatacgacgc tgaaaaccta gggctaagct gcttaaagag gttattcatc tcggctgaat
    14701 agctacttgc atcggcccga acccctaacg aaaccgttaa ctttgtgaaa aaggttctgt
    14761 tcacctggcc aaaaatattc cacttaaaca aatccatgtt ggtttcataa gtatcggggg
    14821 taaaggtttc accaacaaac tttgctctat aagtggagtt aaaatatcgg gcatactcca
    14881 aacccgctcc aaaatttata ctgtaatttt tcaaccctaa atcgcgctca tagcgtaact
    14941 tattctcggc ctcccacgat gtatagtcga gaaccttggg gaggttatca tcgttattcg
    15001 taaacttgta ctgcctgttg tttagcatat ttcggctaac cacaagggta tggtaaccat
    15061 tgctggcata gtgcttgtaa actgttccaa gggtataatt ccattgtgta tttacaggta
    15121 aataaccaag gatgtaacgt tgatactcat cggggttttt caagcccagg ttcagctcaa
    15181 actcatcaat ggcaccgagt ccaattacgg taatctcatt ttttgcatcg atacgggttt
    15241 tagtcttgaa ctgaaagtcg ttatagttgg gcaaaaacgg aagtcctaag gcgttaaaaa
    15301 ggaattgcag gtatgagcgg cgagccgaga aaatcatggt agtattattg cttaatggcc
    15361 catcgagggt cagcgctaaa tccgatgctc ctactgctcc cttaaccttt agtcgctcac
    15421 tgtttccatc aacctgacgg aaatcgagta ctgaactaag cgcgttgccc tttgaggctg
    15481 ggaaagcgcc agaatagaag tttacctcac gcacaaaatc aacattaatg atgcctactg
    15541 ggcctccgga agccccctgt gtggcaaagt gattcaggtt tgggatttca acaccatcga
    15601 ggtagaacct gttctcattt ggccctccac ccctaacaat aacatcgtta cggtaagcaa
    15661 gcgagggagc aacgccaggc aacgactgaa taacgcggct aatgtcgcgg tttcctcccg
    15721 ggttacgctc aatctcatcg ataccgatac ggcgtaaaga aagcggggct tcctcgcttc
    15781 gcctaaaagg cgatgccttt accaccacac tctcaatctc atagggcatt tcctccagtg
    15841 gaatatcgat aaaaatattt tttgagcggg ttaccattac tgcttcggaa atgtagggtt
    15901 taaaaccaat ggcagtaacc tgtagctcaa cccaccccgg ctccagaccg gcaataataa
    15961 aattgccatc aaagtcagag gtggtgccaa taatttttcc atatacaatt actgtggcaa
    16021 atggaattgg ttcgttggtt ttactatcaa atactctccc ctttatcata cccttatcct
    16081 gggcattgag tgggtttaat agtacaatcg atataaaaag caataatgtt ttacgtaaca
    16141 tagcataaag aattttgcat aagaaacgca tcgcagcatg ttttgtttaa catttgcgat
    16201 attgccttat ataaaaatag ctaaattttc aattccccta aacaaaccta cctgtttttc
    16261 tcaatgtctt taataagcag gtagttcata catgcggtaa gcgccttagc cctcaacata
    16321 agtgagtcga gatgtttatc gggtgaatag ttgcttggct caagcttacg atgttcccta
    16381 tcgctccacc gataaaacaa aggggttgag ctaaaatcga ccagaccaat gctatcggca
    16441 gcaatgctgt agcagtaaag tccaaaatcg tttgcacaat aggtgttaca taggttgttt
    16501 ccaaagtttg gatatcgcgc acttgaaagc gaaaggttgt aaagggtaga aaaaatgtcc
    16561 ttatgcgatg caaaacgcct ggtattgatt ggatttttag gacgatattt aggtggaata
    16621 tagatgagta atggaactga gtactgcata aacaaatcgg cctgcggata ctcaaagttc
    16681 tgtcggatat tgtggtcgcc agtggctaca attatagttt tttcgccaag gggggaagta
    16741 ataatacgtt tgataaaatc gcccaaacag ctattggcgt actggtatgc cctaagacct
    16801 ttcaacacta ttgcctcgtc gaacttcatg cgtttgcgcg cccaatctgg aattgaaatg
    16861 gggtaaccct ggtagttgtc aggaatgtcg tatggagtat ggtgcgaaat ggtcataccg
    16921 aatatcaggt aaggtttgcc attagaattt gcaagcacat cgaaagccct ttggaaaagc
    16981 acctcatcgt gcatgcccca ctccccttcc ttgccctcgg ggtataactt cctgagggtt
    17041 ggttctgctt caatgctatt aaaatactga cgtggtataa acttatccat attcctccaa
    17101 cccattttac ctccggttaa aaatatattt gtatatccct tttcataaaa aggctttgct
    17161 gccgaagcat caaatgaaaa gttctgataa ggtgactgag atagtggact tttgggagtg
    17221 tttacaagta tactttctaa ggagtagatt gttcctgagt acgatggtaa aaagttacgg
    17281 aacaggtagc agctatccaa aacatcggca agggaaccca acatgttgca gctatcggaa
    17341 tgcatatcga tataaaagtt actcatgctt tccattagga taaaaaccac atgcggcgga
    17401 ttattttcaa gaaaatggtt ttgaggagtg gtggtgtaaa aggtttcagc tgatacacta
    17461 tcgaccttta aaccataaaa aacacctgct gcatcctcta ttgatttgaa ttttaaggac
    17521 ttgagcattt taggtatatt gatgtcaatc cggctccttt gcctatcagt ccacgcaatt
    17581 tttgtgctga aaagaccgtt aacaggcaac gtatttacaa aaatattttt agatatgatt
    17641 gtatgctgct catcaatcga agtaacgcct atgttgccaa gcattcccaa aaagtataag
    17701 cccgaaattg caaccacaat taatactcgc agccaaatag gccttaacct cttgcagaag
    17761 ttgcaactta tgggcttata aagatgcacg aacagcttac taaaagtcca aatcaacact
    17821 ataaacaacg ccagaatcgg gattacggga tattctttcc aaatggaaac tagcacagcc
    17881 caggtatcat cctcaaaaaa accaaaaatt attacgctaa tctttgtatg gtagaactta
    17941 taaaagtaaa agttgattac ctcaaacatc accaccaaaa tcataagtgc agtagagtaa
    18001 tacctgaaaa actttgtaaa gttccccacc ctatcgctaa aaatcaatct aacaaaagcc
    18061 agtaataaac atggtaacag ggcaatagtc agcactttta ggtcgaactt taagcccacg
    18121 gtgaaagcct ttgcaatatc gcccgacaat ccactaaggt cgttccaatt agcaaacgaa
    18181 aacagaaata ttaacctcga aataaaaagg attagtactc caaccgctaa gttttttact
    18241 atccaaagaa tagcctcaac aaagctatcg aaacccgttt tatctgtcat actaaactag
    18301 cctgttattt tcaaaagcgc tatcaactat tgtctttacg ttgggtaaac ctttaatgga
    18361 attgctaaaa agggcaaggt attcaatatt atagccctta cccttgaggg gtgcaaaggt
    18421 aatactattc aggtagatgt tatgctgagc aaagcacttt gccacgttct caattacaac
    18481 ctcgtgaacc tttttatcct ttaccacccc atttttacca atattttcct ttcccgcttc
    18541 aaactgtggc ttaatgagca aaaccataaa cccatcgggt ttaaggaatt tcactaacga
    18601 ttgagctacc tgcgtaagcg aaatgaacga aaggtcggcg gtaattacat caaccctagt
    18661 acccaatgcc gagatatcca aggttcgaat gtcagttttt tccaaggagc agacccgtga
    18721 attgttaatc aattctgggt ctaactggtt ggttcccaca tcaacagccc aaacaaacct
    18781 tgcaccatgc tttagggcgc aataggtaaa gccaccggtt gaagccccca catcgagcac
    18841 cagtttgtct tggaagttga gcccaaaatc gtctattgcc ttttgaagct taagttcccc
    18901 gcggctaaaa aaatcgttgg ggttaatgga gagctgaata gagtcggttt cggctaccgc
    18961 aaacgatggt ttagtaacct ttttcccgtt caccagaacc tcgcctgcgg taatatgcgc
    19021 ttttgcgcgc tcgcgcgagc ggcataatcc tttcttcacc aaatatatgt caagccttac
    19081 cattccctgc ccttgtcttt tttattcgcc gaaccatagc tttaaccccc tgagttgaat
    19141 cgatttgaat aagccttagg gtattaggca actcctcggc aatccaggta tattgatttg
    19201 atagaatcca cataatttca aggcaatgaa ccctaactgc tacgggaaca tcggtgctaa
    19261 gcaaccagcc atagcaagcc tctatcaatc cctcaacctt gctattgcgc gcttcaatcc
    19321 attcagtaag tttattttga ttactttcca accatgtttt gaacagcttg cagaaatggc
    19381 gacgaactga gttgttttgc aaactgggaa taacagccag taacctatcg gcatggacat
    19441 ctaaaagcga ggtattatcc aataagcagt tctccagaac ccaagctgca tggaaagcca
    19501 actggctatt tttggacaag cttagttcaa aaagcgtatc agttccattg ggtatggcag
    19561 aaaaaaaaga tgccagctgt tgcacttcgc ttttacgaag tgagtatttt agccgttccg
    19621 ttaggttatc gatttccatc agcaaaaatt gcgaaaccac aaatttagtg tatggccaac
    19681 aattacgata tactcgaaca gctttttgat aaggcaaaga gcataaaccc ctttagcgat
    19741 gggcatttta cctttggtga aaagtatgtg gcagttcaga acggactggg acaaataggt
    19801 gtatgcgcca cattaggtaa aaaggtagac cacacaaacc ttgaaaattt tgaccttggc
    19861 aaagctgaat gccgggttgc tgctaacgca tttgccaacg caaccctaaa ctaccttgta
    19921 gaacccgacg gggttggtga tatttttgac acggttgatt ttagcagcca taccaacctg
    19981 gtgatgatag gctactttgg ctcactggtt cagaagctgc aaaccaaggg tatcaaaccc
    20041 ctggttttcg acatcgacca gcacgaagcg ccagtgctac cgatggataa acagcacgaa
    20101 tacctcacgg ttgccgactg tgttattctc acctcaacca gcctaagcaa tggaacattc
    20161 aacgggattg taaaaaccgt aaacccgcaa tgctccatat acatgcttgg cccctccacc
    20221 ccgctggacg acctgatgtt taccattcca caggtgaagg gactatttgg ctcaattttc
    20281 ccgatccata gctccgaaac cctcaacctt atagcccagg gttacggtac caagggcttt
    20341 atgcacacca tgcggaaggt ttacaggata agaaaatagc tattacgaaa aaacgcttag
    20401 ctacataaag aagctaagcg tttaagttaa tacttgatat ttacctatta ttgcaaaatc
    20461 accatctcgt taatcaaatt cttagcaccg gcatacttat cgataacaaa aagcgcgtag
    20521 cgtatatcga gcgatatgtt acggcagtag ttggggtcaa acattacgtc gctcatggtg
    20581 ctttcccagc agcggtcaaa gtttacacct ataagattgc cttcggcatc gagcacggga
    20641 ctaccggaat taccgccagt tgtatggttt gaggcgatga atgcaaccgg aacagttcca
    20701 ttaacagcat aggggccaaa atcctttgtc tggtaaagct ctatcaaccg ttttggcgga
    20761 acgtaatcgg gaacgtcgcg ctgctgcgat ttttcgtaaa tcccatcgat agtggtatag
    20821 tagtcgtaaa ccacaccatc gcgcggggca aaaccgccca ccttgccgta tgcaatgcgg
    20881 agggtagagt tggcatcggg aaagaatcgc ttatcgggtt gcatacgcat taacccttcc
    20941 acgtaggttc tgtaaaggat atccagacta tcggtaattt caaagtagcg tttttgcaca
    21001 ttcttacggt aaaggtcttc gaactcaaag tagatgctaa agattgggtc gttcaggaac
    21061 tcacgggcat tggtgctatc aatgtcattc aaaagggtta aaacccttac gctatcggca
    21121 aaagcggttt gtgaatagat ttgcttagct aactcccgcc aattacccga ctgctggatg
    21181 aacttaccta aaaattcagg ttgaagctgt tggggaatac gctcgtagta ggcttgcatc
    21241 atagcagcaa aaacctccct gtcaacactg agttcaaagt ccttgaagaa atcgcgaaca
    21301 tactccacta gcttattacg ctgttcagcc aatttcccct tatcaacacc ggggtcaaga
    21361 gccatgctca acaatcgctc aaaacgcgat gcaaagctga gcatttcaac cccataaacc
    21421 gcctcgttgc ggtaatccac aaccagcgac agaggagcta actcagcgta aagcctatcg
    21481 aaggtaggaa gtaaccaacc gtaacgctcc ttaagctcag ggtttgtagc cgcccacgca
    21541 gcaaacatac tctcctgctc ctgctttttg gaaacagcat ccaacctgcg caaacccatt
    21601 gtttcaccaa tccacttttt ccaggcattg gccactcggg catgcttagc cgcatactta
    21661 atggcaatag tatcgtttga ctcggtaaac ttattgaaaa ttgaaaggcg gatatcgcgc
    21721 agatttattt tatgcgggtt gctttggtca ataagcaacc taacggcatg ggaggttata
    21781 aactgctgag tgcggccggg aaagccatag acaagggtaa aatcgccttc gttaacacct
    21841 ttcatcgata ttttgaagaa ttttttgggt ttgtagggaa cattgcttgg tgagtatggt
    21901 gctggattat tgttcttatc ggcgtagata cggaatagcg agaaatcgcc agtatggcga
    21961 ggccaaaccc agttgtcggt atcgccacca aacttaccta ttgactctgg gggtgtaccc
    22021 accaggcgca catcttcaaa cacctggtaa acaaacatgt aatactgatt gccatagtaa
    22081 aaaggacgaa tgctagcttc gtaaccaatg ccttcctttt cagcttttgc aattaggcta
    22141 tcggccacct gctgaatgct ctttgccctt tcctgctcgg tcatagatgg ggctatgttt
    22201 ttcaggacta aattggttac atcgtccatg cgcacaagga acctcacctt gagacccgga
    22261 ttgggcaatt cttcggcttt actgctagcc caaaagccgt tggctaaaag gttgttctca
    22321 accgttgagt gcgattgtat ttcagggtat ccgcaatgat ggtttgtaat cagaaggccc
    22381 tcagccgaga taagttcacc ggtgcagccc ccgccaaaat gaactatggc atccttaagg
    22441 cttgccttat ttacgctata aatatcctcg gcagtaagct taaagcccat tcgttgcatc
    22501 tcggcaatat ttttaccaat aagcattggt atccacatgc cctcatcggc cattcccctg
    22561 aaagtaaggg acacagcaag cagtattaac aaaaatgatt ttctcatagc tattcggatt
    22621 tttctacttt gacagtgaat tgataaaaga gattaattgc gagtgcagcg tatgaatggg
    22681 taaccccatt acgttaaagt atgaaccatc gatgcgttca acccccacgt atccaatcca
    22741 ctcctgcacc ccgtaagctc cagccttatc gtacggttta tacctgctga tgtagtaatc
    22801 tatctcctcc atacttaagc gtttgaaatg aacctcggtt gcaacatcga aaaccctttc
    22861 ggaatttttg gatttaaggc acacgccggt aataaccgta tgcgacttat tggacaaagc
    22921 gcttagtatt cggcgggcat cggcttcatc ctttggcttg ccaaggattt ggttgcagca
    22981 aataacaatt gtatcgcagg tgataaggat tgtgctatca tcgagcctat cggctaagtg
    23041 tgtagccttt tgctttgcga ggtaaaccgg cacatcggcc aaaggaaggt tggcaaggaa
    23101 tggctcctca tcgtcgtgat ttgtatccca tatctcaaat tccacatcaa gccctttaag
    23161 cagctcacgc ctcctaggcg atttagatgc cagaactagt cgtttcccct ggagcaattc
    23221 atgtagcaac atcaacaaaa aactttacag gtttactata aaacggaaaa tgagtgtgta
    23281 aatcagccct aaaagcatga taatcttgca tagcgaactt gccttatggt aatccttctt
    23341 ggacgatgca agcaatacgc taaaaatcaa aataaaaatg gggattacta tgaatatcac
    23401 caggtaaact aacgaaacat aatcgaaagg cttaaccgaa agcttaaaaa gatatgccga
    23461 aaaggcaact aacagggcaa tcagggttaa cgacaacact gtagcaataa tcaattttga
    23521 agttttgacc ccaaagtgaa cgggtattgt gtttcgccca agcgatgtat cgccttcaaa
    23581 atcttcaata tccttaataa tttcccgtgc aagagtaagc ataaaggcaa aaaaggcata
    23641 cccccctacc cagtaaacaa tcaccttcat gttaagctgg taaatgaaaa tgacatcgct
    23701 gaaggttcca agcaagggga actcaaaaat cagggggatg agcggaacca ttgcagttaa
    23761 aaacgcaacc accaggttac ccacaaatag ctgacgctta taggtagttg agtaaaacca
    23821 aagcacaccg gcagtaagga agaatagtaa cgaaaacact ggtcttccca cacgaaacga
    23881 aactaccgtg cccagaaaaa tcccgagaat ggaaagcact aaatggaaag caattgctcc
    23941 ccgacgggtt acctgttttc caacaaccac cgtttcggga cgattaatca gatcggcttt
    24001 agtatcaaag taatcgttta taacgtatcc gccggctgca accagcatgg ttgcaagaac
    24061 taaggctaca aaataccaaa tgggcatctg taattctaat ccataacctg aaagtatggt
    24121 ttgtataagc atatacctaa ccagcaccat tgttgctgca ataatcagca agttcttgaa
    24181 ccggactaat cgaaagtatt taagcatagc acaaatgttt tttactgtaa cccgtttgcg
    24241 gatgggcccc atttcccatt gacccttaac acctgctcaa ttacatcgcg cacacagccc
    24301 ttgcctcctg caaaactcga aatgtaggct gaaatttgct gtatttcagg tgctgcatcc
    24361 cttgggcagg ttgggattcc tactcgttgc agaacctcaa aatcgggtaa atcgtcgccc
    24421 atgtaaagta tatcggagag ttcaagccca tacttaaacc gaaaatcctc aagagcctca
    24481 attttatcgg tagtaccaag gtagatatcg gttacaccta atccctgaaa gcgttggcga
    24541 accgcctcgg acttgcctcc ggaaatgata gccaccggaa aaccacgttc tatagcaacg
    24601 tgaacagcat agccatcgcg cgtgttcgag gttctaagta gttcacccga tgggtgaatg
    24661 ataattgtac catcggtaaa caccccatca acatcaaagg caaatgcctt gacatgacta
    24721 agtaattcct taaaattctt tttacccacc ataaagaaag attatgccta tgtacggttt
    24781 tttaaccctt tgtcacttcc tgacaatttt tgaatgctca aagatagctc tttgtacaaa
    24841 tcggccaagc caaatacttt caacatctga gcatggcgct ccatggtttg aatatcgtta
    24901 cgtacagcgg gaccggtttg gtaaagtgcc ggattcccct caaaagcctt gcgaatggtt
    24961 tcctccacaa gaggtttaac aacctcaaaa ctaatatgat gctccgtggt taattggtat
    25021 gccaaagcca acatgtggtt tgtaaaatta catgcaaaaa cacctgaaag gtgtaaccac
    25081 cttcgctgct ctgaactcat gggaactata gttcccgaca attttgaggc aaactgctcc
    25141 agggaagcgt agcattcatc gttacttgcc tcaaggcaaa taggaatgtt actaaaggga
    25201 actatcttac tccgcgaaaa ggtttgaaaa gggtaaaaaa cgccaaagcc cttagccctc
    25261 aacccttcaa aaaccgacat gggagtactt ccactagtat gaacaacaca accctttagc
    25321 tcaggcaaaa ctgaaacaat actggcaata acatcatcgg gaagagcaac aatatagata
    25381 ctcgcactgg ttctcaaact ttcaagtttg caaattggct cagccccaac aaggtttgcc
    25441 aatttacttg cactctccac ggttctggag taaacctgta ggataggaaa ccctgcattg
    25501 cttaacgcaa cagccaacga ggttgccaaa ttgcccgcac caagcagtac cacatcgtgt
    25561 atcatggcgt agatgtaatt tgaggtataa aggtaagaaa gaaaaatgaa aagaagtccg
    25621 tgaaacgtga aaagaagaat tgagaatatg agggagttag aggaagttag aggaaaagtg
    25681 aatagtgaaa agtgaaaagt gaaaagtaaa accaacgaaa atgagtcgga atgctcggaa
    25741 tgagtcggaa tgagtcggaa tagacagaat atgatggagt tagaggaagt tagaggaagt
    25801 ttgagtccgt gaaccgtgag ccgtgagccg tgagccgtta aacgttaata tgtgactttt
    25861 agcgggagtg aatcggaatg agtcggaata atcggaatgc tcggaatgag tcggaataga
    25921 cagaatataa tggagttaga agaagttaga ggaagttaga ggaagtttga gtctgtgaaa
    25981 cgtgaaaaga agaattgaga atatgaggga gttagaggaa gttagaggaa aagtgaaaag
    26041 tgaaaagtga aaagttaaac tcaacgagaa tgagtcggaa tgagtcggaa taatttggaa
    26101 tggacactct ttaaccaaca actgtcaacc aacaactgtc aactaccagt taaaccttaa
    26161 actcccagca tgctctttac cattgccacc aacctttcgc catttattgg cttagtgatg
    26221 taggcatcac agcctacct
//
LOCUS       DAOSAL010000030        25140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_1421_length_25140_cov_49.442695, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000030 DAOSAL010000000
VERSION     DAOSAL010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 25140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25140
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1421_length_25140_cov_49.442695"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            271..1230
                     /gene="trxB"
                     /locus_tag="PK049_05885"
     CDS             271..1230
                     /gene="trxB"
                     /locus_tag="PK049_05885"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOK61122.1"
                     /translation="MESAGKELPVENVRVLILGSGPAGYTAAIYAARANLKPVLYEGI
                     QPGGQLTTTTEIENFPGYPEGITGTEMMEDLKKQAMRFGTDVRFGIATSTDLSKQPYR
                     VVIDDEKVIETDALIIATGATAKYLGLESEEKFKGMGVSACATCDGFFYRGKDVAVVG
                     GGDTACEEATYLAGLCNKVYMIVRKPFLRASKAMQDRVFNTPNITVMFEHVTKEIVGD
                     QSGVNGVILTNTKTGEDVKIDISGFFLAIGHHPNSDIFKPWIETDEVGYIKTIPGSTK
                     TNLPGVFACGDVQDPHYRQAITAAGSGCMAAIDAERYLGERNF"
     gene            complement(1301..2539)
                     /locus_tag="PK049_05890"
     CDS             complement(1301..2539)
                     /locus_tag="PK049_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOK61123.1"
                     /translation="MDIFDKINNMGPIGQWQQYSHGYFSFPKLEGEIKPRMIFRGKEV
                     LTWSLNNYLGLANHPEVRKADAEAAAKYGMAYPMGARMMSGQTTKHEELEQKLAKFVG
                     KESAYLLNYGYQGMVSIIDTMLNRNDVVVYDSESHACIIDGLRLFPGKRFVYQHNDMD
                     SCRKMLQHATKTAEKTGGGILLITEGVFGMAGDLGNLKAIVDMKKEFNFRILVDDAHG
                     FGTMGKTGAGTPEHFGVQNEIDLYFATFAKSMAGIGGFVASNEKVIRYLAYNMRSQIF
                     AKSLPMPMVFGALKRLELLQTQPELKENLWKVVKALQSGLKEMGFDIGKTESPVTPVY
                     MKGGIPEAANMVFDLRENYGIFCSMVVYPVIPKGQILLRLIPTAVHTLDDVKETINAF
                     AAVREKLLAGKYDKEKIAQI"
     gene            2800..3615
                     /gene="bamD"
                     /locus_tag="PK049_05895"
     CDS             2800..3615
                     /gene="bamD"
                     /locus_tag="PK049_05895"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOK61124.1"
                     /translation="MKIQGLFKFERIVLLSFIIVLLSSCSGFEKVLKSKDYEYKYKMA
                     LEYYKKKDHYRYVTLLEQLQSVYKGTFRADTIDFYLAQGYYHQGDYLLAGHQFDMFRK
                     NYQRSVFTEEAEFMYAYCYYMSSPRPELDQSNTLAAIEAFTEFINRYPNTSHKAEVNK
                     IMVELRDKLVEKSYLSAMLYYKIGDYKAAIVAIRNSIRDYPESKHREELMFHLLKASY
                     LLADNSIESKRRERFQNALDEYYSFISEYPSSKYSSEAKKMYDATMKVLQNLN"
     gene            3630..3968
                     /locus_tag="PK049_05900"
     CDS             3630..3968
                     /locus_tag="PK049_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOK61125.1"
                     /translation="MDIKKINAPVNTVTRDLDKLDRETGNIYQSVRIIAKRANKISID
                     MKQELNRKLEEFAYYTDNLEEVFENREQIEISKFFERLPKPTLIAAQEFLDGKLHYEF
                     KEDQTSESEA"
     gene            3958..5166
                     /gene="coaBC"
                     /locus_tag="PK049_05905"
     CDS             3958..5166
                     /gene="coaBC"
                     /locus_tag="PK049_05905"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOK61126.1"
                     /translation="MRLKGKHILIGVTGSIAAYKAATLIRLLVKEGAEVKVLMTPLAK
                     EFITPLTLATLSKNPILVDFFNPENGDWNSHVDLGLWSDLYIIAPASANTIAKMATGV
                     ADNLLLTTYLSARCPVMVAPAMDLDMYRHPATQQNISTLQARGNIIIEAATGELASGL
                     SGKGRMEEPENILEQVVSFFLTSQRLSGVRVLVTSGPTYEHIDPVRFIGNHSSGKMGN
                     AIALELARQGAIVDFVSGPVSQHPNHPNINIHRVTSAAEMYKAATTLFGEAKVAILAA
                     AVADFTVENVANQKIKREADDLVLRLKPTNDIAAHLGKVKKSNQLLVGFALETANEIE
                     NAKAKLQRKNLDMIVLNSMNDPGAGFGTDTNKVTFIMRSGQVVPFETKPKDMVAIDIV
                     DAVCEMLNKG"
     gene            5172..6083
                     /locus_tag="PK049_05910"
     CDS             5172..6083
                     /locus_tag="PK049_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HOK61127.1"
                     /translation="MNRFVGLLLVLLPFVANSQELRCNVSVNSQRIQGTNRLLFQNMQ
                     TAIYEFMNNTPWTNHVYGFDERIECSIMLNITEQIGSDEFKGTIQVQSRRPVFNTSYN
                     TTMLNFQDNNLHFRYREYEKLEFSESSHLSNLTSILAFYAYIIIGLDYDSFSKLGGTE
                     YFQKAERIVNNAQNAVERGWKAYEGNRKNRYWMIENLLNPKYQPLREALYRYHRLGLD
                     VMSEKAAEGRAEIAESLALLQRVYREKPDPYLFALQLFFDAKNEELVNIFSESYPTEK
                     ARVVNILTEIDNPNAAKYKRIIEQNTQ"
     gene            6097..7767
                     /gene="recN"
                     /locus_tag="PK049_05915"
     CDS             6097..7767
                     /gene="recN"
                     /locus_tag="PK049_05915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOK61128.1"
                     /translation="MIRKLYIQNYAIIDELEIEFKQGLNIITGETGAGKSILLGALSL
                     IQGQRADTSVLKHNDRSCIVEVEFDISEYNLNDFFEANDIDYDDITTIRRQISENGKS
                     RAFINEIPVNLNQLKELTEKLIDIHSQHQNLLLGNTRFQLDVLDAFAVSRDLLTDYRQ
                     LYDEFRKLKTELDRLEANAENAKRDFDYLSHQLNELNAANLRSGELDELEALQKQLTH
                     ASEIKTALEFSYGTLNADELAVLSKVKEIESNLRRIESVFSQANNLISRIESCRIELK
                     DIADEIDLLNSKVEVDDEQLHTVTQRIDLIYSLLNKHRLANFDELVEFRNQLESRVNE
                     IAQIDFNLDEKRKALRILEQKLQEKSKNLSQIRASSAPKVEQFVTELLQQLGIKHAVF
                     RIRIEKLEEFQPWGTDRVTFYFSANKQIEPQELSRVASGGELSRLMLSLKSLLVKSTG
                     LPTIIFDEIDTGVSGEIADRMGTIIYELAKGMQVINITHLPQIAAKGTNHFLVYKSVT
                     NSQSSTGIKLLTPDERVVEIAKMLSGEKVTDAALMNAKELLKSNVSLS"
     gene            7826..8665
                     /locus_tag="PK049_05920"
     CDS             7826..8665
                     /locus_tag="PK049_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HOK61129.1"
                     /translation="MAYNLLKGKKGLIFGALNEKSIAWKVAERAFEEGAEVVLTNTPV
                     ALRFGNVYELAERMNTVVIPADATNIQDLENLIDQTMERFGGKIDFVLHSIGMSPNVR
                     KGRTYDDLDYEFFLKTLDISAISFHKILQTCWKKDAITHGGSVVALSYIAAQRTLYGY
                     NDMADAKALLESIARSFGYIYGRERGVRINTVSQSPTETTAGGGVMGFDALLDFSDRM
                     SPLGNASALDCADFCITLFSDLTRKITMQNIYNDGGFSSMGMSNRAMAVYNRNLEECK
                     DCK"
     gene            complement(8714..9574)
                     /locus_tag="PK049_05925"
     CDS             complement(8714..9574)
                     /locus_tag="PK049_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HOK61130.1"
                     /translation="MRQLKITKSITNRESASLDKYLQEIGKEDLITVEEEVELAQRIR
                     KGDQAALEKLTRANLRFVVSVAKQYQNQGLSLPDLINEGNLGLIKAAEKFDETRGFKF
                     ISYAVWWIRQSILQALAEQSRIVRLPLNQVGSLNKINKAFSKFEQEFERTPSPEELAD
                     LLELPKEKVNDTLRASGRHVSVDAPFVDGEDNSLLDVLVNNDSPSADRGLINESLSRE
                     IERALATLTERERDIIKLFFGIGCQEMTLEEIGEKFGLTRERVRQIKEKAIRRLRHTS
                     RSKLLKSYLG"
     gene            complement(9732..11177)
                     /locus_tag="PK049_05930"
     CDS             complement(9732..11177)
                     /locus_tag="PK049_05930"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HOK61131.1"
                     /translation="MKAQKFILPVIYATLGGMLAIILSNHVLSSNNNEGVVTQKTPAQ
                     VVRAFAPDSSSTDFTWAAERSVDAVVHVTTEFTQSVDYSFGNPLFDFFFGPRDYMPRQ
                     KVQSSGSGVILTHDGFIVTNNHVIENADEITVVLNDKRKFTAKLVGTDPSTDIALLKI
                     DATDLPFLSFGNSDNIKIGEWVLAIGNPFNLTSTVTAGIVSAKARNINILSGREFAIE
                     SFIQTDAAVNPGNSGGALINLRGELIGINTAIASRTGSFTGYAFAIPSNIVKKVVTDI
                     IEFGEVQRAILGVRIQELTAELAKEKGIKEIKGVYIFDVEKGGAADAAGIKPGDVILS
                     VNGVDVNSTAQLQEQISRYRPNQQVDIVVNRDNSKKRFNVTLRNLKGGLGVVKTDDTL
                     SSLGAEFKEITDKQKQELGINYGLQIVELQDGKLKEGGIKKGYIITRMNRTPIRNIDD
                     LKRVLSISSGGVLIEGVYPNGVVAYYAIGVN"
     gene            complement(11354..11728)
                     /locus_tag="PK049_05935"
     CDS             complement(11354..11728)
                     /locus_tag="PK049_05935"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOK61132.1"
                     /translation="MKPLKLLETSDTPEVTLDKERGRFEFYGKIMPENPKEFFEPIIE
                     WFHEYLKEPNQETNLVFKLDYFNTAASKKIIEILSLLQNIKKENKAVQVNWYYKSIDE
                     DMLEVGETFSEIIHIPFNFYPY"
     gene            complement(11810..12589)
                     /gene="dapF"
                     /locus_tag="PK049_05940"
     CDS             complement(11810..12589)
                     /gene="dapF"
                     /locus_tag="PK049_05940"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOK61133.1"
                     /translation="MMLQFFKYHGAGNDFIMIDNRDQAFTPMVQGVKHLCDRHYGIGA
                     DGLILLETQNGMPFMRYFNCDGNESTMCGNGGRCFATFAKKIGLANGLSFDFQGIDGM
                     HSAIFNTDGTVKLKMIDVETLEIIDDCYFINTGSPHYVTMVRDVNGVDVDREGRLIRQ
                     SVNFNNGGTNVNFIQEVAPGRIKIRTYERGVEAETLACGTGATASAIAYSHYFDINQT
                     PIEVEALGGLLKVYFDQIEEKYLNVWLEGPAEEVFEGKINY"
     gene            complement(12676..14544)
                     /locus_tag="PK049_05945"
     CDS             complement(12676..14544)
                     /locus_tag="PK049_05945"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOK61134.1"
                     /translation="MKQIKLIFLLFYVMATAIHAVGQQEIVTIPKEKQDDIDRYKELV
                     VRYKQANNSQQAAFYLNKIAFIYWEFGNPKEAINYFLETIPLNEKVGNYNDIKAVYSN
                     IALIYSDMDRLDLTLEYFYKSLEARRKLNNKGEVAAGLIDVAYIHTALNQSEKAIQLL
                     EEALELSREVNNPRLILNSLKLLAQNYDNLGNKAKATEYGAMANAYEQQLATQQIKTE
                     YETKVVKSQAEAERERMQRTQQELLMSLRELQSKAMQDSLNFVVLRKQDSLRKAEEEA
                     QRKQTEIDLLNTDRMLKEAQLREREAQSRVQRIIIFAGAIFVLVLIISSVAIYKNYTD
                     KKKANELLNLQNIEIQQQRDQIQKQNENISKSINYAQGIQRALLPPQSNLQAIFPESF
                     IFFRPRDVVSGDYYWFKELTTGYGSNEKTGKVAVSAIDCTGHGVPGAFLSMIGYNLLD
                     DIVFRGIHKPGAILTELNNGIRRTLRQDETDNRDGMDMALCVVDTKTNIVEFSGAKNP
                     LVYVVNNEVNRIRGDKESIGGGMDYRNDEFTTHVIKVESPTWFYMFSDGFIDQFGGPD
                     GRKYMIKNFIDLLAGISILPPDQQREILKNTLKDWLGTKYPQVDDILVVGFKIDPK"
     gene            14837..15688
                     /locus_tag="PK049_05950"
     CDS             14837..15688
                     /locus_tag="PK049_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61135.1"
                     /translation="MLKRNLFYIIGGLLFSNLALGQDFEVAPVRLEFDAEPATSQMKT
                     ITVKNHSSRRVSYTVSFADFLPTTSGERKILPPNSTKRTCANWINVNPAFFELNPGED
                     IQIQVNMLVPGEEFGTAWCMLFIQPTREQTSWSADKQLAAGITVSGRIGVLVYQSPKS
                     NQNYSIKVSNFQEVTKPGDPQRKFSAVIENLGDKVAHCKVFLLASDMLTAEEKQFPPK
                     DFEIFPKLTRTVEMELPNDLPAGKYALAIIVDYGPKYPLEGSQLVIDIAGEIPKADKT
                     DSTKVNP"
     gene            15797..16858
                     /locus_tag="PK049_05955"
     CDS             15797..16858
                     /locus_tag="PK049_05955"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HOK61136.1"
                     /translation="MVKEKFQGVVVNGDTLRQGRYLYFFDNGVLNQEGFYTNGKPDSV
                     WITYYPSGARLSLFHYNSGKKDGPFLFWNSDGTLYQQGYYKNDLLDGVLETYYPNGKL
                     SAKSEYSKGRLNGISEVFDESGLLRLRSGYKNDTLNGLWETYYDTGELNYKGTKCLGN
                     YCDTLYTYYKNGVLMRKANYSNGRIDGVLISNYENGVLQIKAEYRNGSQDGNYTEYYP
                     DGSIKETGSYLMNQKVGWWTSYYQGGQKHGQGNFSKGVLDGEWIFWWPNGNVKQHGSY
                     CKGKACGRWKYFNSMGALIIDGNYIDDKENGIWTTFYPNGAIAGRIWYLRGKPVSVKW
                     LYDGQGLLQKRFSLEGLEK"
     gene            complement(16884..18863)
                     /gene="gyrB"
                     /locus_tag="PK049_05960"
     CDS             complement(16884..18863)
                     /gene="gyrB"
                     /locus_tag="PK049_05960"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOK61137.1"
                     /translation="MSDKEKELKGENGSASYSASSIQVLEGLEAVRMRPAMYIGDTGN
                     KGLHHLVYEVVDNSIDEALAGYCNRIDLVINEDGSITISDNGRGIPVDFHEKEKKSAL
                     EVVLTVLHAGGKFDKDSYKVSGGLHGVGVSCVNALSVNLIAEVKRDGKIYRQEYSRGK
                     PTTELKVIGSTEETGTTITFMPDSQIFTQTTVFNFEILESRLRELAFLNKGIKLTLTD
                     KREIIENGTRSYRYAEFYSQDGLKEFIKFLDINREPLIENVIYIENDKGEVPVEVAMQ
                     YNTSFSENVHSYVNNINTIEGGTHLTGFRSALTRTLKKYADDSGMLSKLKFEISGDDF
                     REGLTAVISVKVAEPQFEGQTKTKLGNPEVRIAVDQAVSEALANYLEENPKDARNIVQ
                     KVILAAQARHAARKARELVQRKTVLSGSGLPGKLADCSDKDPAVSEIFLVEGDSAGGT
                     AKQGRDRRFQAILPLRGKILNVEKAMQHKIFENEEIKNIFTALGVSIGTEEDSKAINL
                     SGLRYNKIIIMTDADVDGSHIATLIMTFFFRYMTDLINKGHLYIATPPLYLVKKGKEE
                     RYCWNEEERLKAVEELGKGKDGSVHVQRYKGLGEMNAEQLWTTTMNPETRILRQVTIE
                     SAAEADRIFSMLMGDEVPPRREFIERHAKYANIDA"
     gene            complement(19083..20753)
                     /locus_tag="PK049_05965"
     CDS             complement(19083..20753)
                     /locus_tag="PK049_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOK61138.1"
                     /translation="MRSETKYIVIPLTAAMFLMAGSHAAAQTNDLKKEVQVVRPYEPS
                     ISDANKINLQPKIIDTIKVNPNFTYSIIQRPIETYFTPTPLSAARMVSEPLPDLHMSY
                     MRVGLGNTALPYAEAYLSSNRNKDYLYGAWVKFFNTSSKLELTNNLKVKAPASNIDIM
                     AFGKRMFNQKILLGDIGFTQNNRTYYGYFFENPALTPSNDKQSINRFAANLEFKTTNK
                     DSASINYHAQTNFYHLTDRYDLQETMVTGLFNLNKYFGQEQFGGEISFTHYGRSNPTG
                     SKNNALLQIKPWAHFFGDQWHAYAGLNIIYDANGNVNNTYFFPRAYLSYDIVSHYIIP
                     YVEIDGYVQENSYSHLLKQNPWLTSSIDTWNTVYKLIVGGGVKGKFSPTISYHVNANY
                     SIIDSLGLMVNTNSDNNNPLFNRFSLLYENTERTTVKGEIAFEPSKSFLVKLNGAFYQ
                     YKTQKISHPWHLPDYEITGFIQYAFRNKLKLNAEVFAVGKRWAKDPNDSPIRLKEYLD
                     INLGAEYVYNKRMSIFVYANNITGSKYQLWYLYPTQRFNMLAGVSYKF"
     gene            complement(20764..>21821)
                     /locus_tag="PK049_05970"
     CDS             complement(20764..>21821)
                     /locus_tag="PK049_05970"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOK61139.1"
                     /translation="LLYYNSSDLENSLAYYKRVVENYPNSPEAKNALIGIRNIYIDKG
                     DAATYFNYASKLGSIANISASEKDSLSYMAAEKVYLTGNCEKSILAFEDYLSENPQGN
                     FAVNAMYYIADCNIRNNNVDKAVEYFENIINRPRNSFTEQSLLRISNIYLNQKQYEKA
                     YTYLDNLESIAEVKSMLLDARLGKLRCAFELQRDDQIPVIAANILSTEKLSPELERET
                     RFKLAKANYRSKKYDDALIEFSRVAQNLKTIEGAESKFMKALIYFERGEYNRTESEVF
                     SFAENNTPHQYWLAKSFILLADSYAAQNDFFQAKATLQSVLDGYSYTTDGIIDEATSK
                     LNQLVKSEKERQSVKQD"
     assembly_gap    21822..21921
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<21922..23969)
                     /locus_tag="PK049_05975"
     CDS             complement(<21922..23969)
                     /locus_tag="PK049_05975"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOK61140.1"
                     /translation="MMKRLLLLVYLIVFFFQIALIAQESSRFNSLNLTYEKGLDLYQK
                     EMYADAQKTFEQYIAGLPKGYYQQKVSAEFYRAMCAIFLNNDDAEYLIGQFIQNYPES
                     QLIDQAYFAMGKMQYQQKKYSQAVYWLSKIDKNGLDKDSRYEWQFMLGYGYFMTNDYE
                     SANRWLFQIKDIDNKFAAPATYYYSHIAYIQKKFATALKGFEKLQNDELFAQLVPYYI
                     THIYYLQGEYKKVTLYSPSLVRNNKNKRAPEVAKIVGDAFFKLKQYDSSLIYLKIYEE
                     KNKQQLSREDIYLLGFAYYKNSKYSEAAKYLERIATIDDSLSQNANYHLADCYLKLDD
                     KNKARQAFGLASKLDFDRIIKEDALFNFAKLTYEQLYAPFNEAIDAFKAYIKEFPSSP
                     RIDEAYNYLTLAYLSSRNYKEAVESLEKISKKDATINTALQRAAYFRGLELFQNLNYT
                     DAIQMFTKSLEYAGYNQQIAAQALFWRAESHYRLSNFENAANDLERFIVTPGAFGLEE
                     YLIAHYNLGYAYFKMKQYDTAITWFRKYNSLLNDSRVNTVADAYNRIGDCFFIQRRYW
                     AAIDYYEKAANLDVLDADYALFQRGFSLGLVSRPEKKIETLKGLLSKYPTSSYTDDAI
                     FEIAEAYNILENTSQAIDFYKRVESEFPNSSYYPKALVQIGLLYYNSSDLENSLAYYK
                     "
     gene            24141..24983
                     /locus_tag="PK049_05980"
     CDS             24141..24983
                     /locus_tag="PK049_05980"
                     /inference="COORDINATES: protein motif:HMM:NF017536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HOK61141.1"
                     /translation="MVTVKAIYNPKRMTEGAGVSIQRILGFETRYECDPFLLLDFFGF
                     SSESEYKAGFPEHPHRGFETITYMLEGQVKHTDSTGGSGIIGPGEVQWMTAGSGIIHS
                     EMPTSDSGKMYGIQLWLNLPQEYKMVDPVYRELKREEIPVVETTSSQVKVISGKYRKR
                     DGGLQGLYKPVDIFDIKLWPNSAFDEVITPEFWNYKYFLFIYEGEFTVAHDLKPIKAP
                     CGVALSQGSIIQIRAGESGAKFLFFGAEPNSEPIAWHGPIVMNTDSEIQKALEELSNG
                     TFLK"
BASE COUNT         7081 a         5051 c         5347 g         7561 t
ORIGIN      
        1 cacctctggt ttttgtatct aattataatt ttagctgata ggcagaggct tgtcaaatgc
       61 atttttttga aacgatccat tacactttaa agctcatggc ctatcaaaaa aatccctatc
      121 tttattcatc aaaaaatatt gtttaaaatc aatttaaata attgtttagg cattgcaaat
      181 tattagtatt tttgcatcaa attgaaatat ctaaattaat aaatatatat agctatggcg
      241 atatttaaat cactaagcac cgatgatgct atggaatcag caggcaagga actgcctgtt
      301 gagaatgtgc gggttctgat tcttggttca gggcctgcag gctatactgc tgcaatatat
      361 gcagcaagag ccaacctcaa gcctgttcta tacgagggta tccaacccgg cggacagcta
      421 actaccacaa ctgaaattga aaatttcccc ggttaccccg agggtattac tggtaccgaa
      481 atgatggaag acctcaaaaa acaggcaatg cgttttggta ccgatgtgag gtttggtata
      541 gctacttcca ccgatctatc caagcaaccc tacagggttg ttatagatga tgagaaggta
      601 attgaaaccg atgctctaat cattgcaaca ggagcaacag caaagtacct tggtcttgaa
      661 tcggaagaaa agttcaaggg catgggggtt tcggcttgtg ccacatgcga tgggttcttt
      721 taccgtggaa aggacgttgc tgtagttggg ggtggcgata cggcctgtga agaggctacc
      781 tacctggctg gactgtgtaa caaggtttac atgattgtcc gtaaaccctt cctaagagca
      841 tcaaaggcaa tgcaggatag ggtattcaac acacccaaca tcaccgttat gtttgagcac
      901 gtaacaaaag agattgtggg tgaccaaagt ggggtaaacg gagtaatact taccaacaca
      961 aaaaccggcg aggatgtaaa aattgatatt tccggatttt tcttggccat tggccatcac
     1021 cctaactccg atatattcaa accctggatt gaaaccgatg aggtgggata tataaaaaca
     1081 atacccggaa gtaccaaaac aaatcttcct ggagtatttg cctgcggcga tgtacaggat
     1141 ccgcattacc gtcaagcaat tactgctgcc ggttcgggtt gcatggctgc cattgatgct
     1201 gaacggtatc ttggggagcg aaacttttaa tgcctctttg atttcaataa aaacaaaaac
     1261 cccgttaaaa cggggttttg ttttgaattt ctgtttacta ctatatctga gcaatctttt
     1321 ccttatcgta ctttcctgcc agcagttttt cccttacggc tgcaaaggcg tttattgttt
     1381 ccttaacatc gtccagggta tgtacggccg taggtataag tcggagcagg atttgtcctt
     1441 ttggaatcac agggtaaacc accatggagc agaaaatgcc atagttctca cgcaaatcga
     1501 aaaccatgtt tgctgcttcg ggtattccac ctttcatgta aacgggggtt actggcgatt
     1561 cagttttacc tatatcaaat cccatttcct ttaatcccga ttgaagggct ttaactacct
     1621 tccaaaggtt ttcctttagt tctggttggg tttgtagtag ttccaaacgc ttaagtgctc
     1681 caaataccat aggcatgggt aacgacttgg caaagatttg cgaacgcatg ttgtaggcta
     1741 ggtaacgtat cactttttca ttcgatgcta caaaaccgcc aatgcctgcc atgctcttgg
     1801 caaaagttgc aaagtaaagg tcaatttcgt tctgtacgcc aaagtgttca ggagtaccgg
     1861 caccggtttt acccatggtt ccaaagccat gcgcatcgtc aaccaaaatg cggaagttga
     1921 actccttctt catgtcaaca atagccttaa ggttacctag gtcgccggcc atgccaaata
     1981 cgccttcggt aatcaggaga attccgccac cggttttctc tgcggttttg gttgcatgct
     2041 gcaacatttt gcggcagcta tccatgtcgt tatgttggta aacaaaccgt ttacctggga
     2101 aaaggcgaag cccatcaata atacaagcat gcgactcgga atcgtataca accacatcgt
     2161 tacggttaag catggtatca ataatggaaa ccataccctg gtagccgtag tttagcaggt
     2221 aagctgattc cttgcccaca aactttgcaa gtttttgctc aagttcctcg tgcttggtgg
     2281 tttgaccgct catcatgcgg gctcccatcg ggtaagccat gccatacttt gcggcagcct
     2341 cagcatcggc tttgcgaact tcgggatggt tggctaaacc caggtagttg ttcaaactcc
     2401 atgttaaaac ctctttcccg cggaaaatca tgcggggttt aatttcgccc tcaagttttg
     2461 gaaatgaaaa atagccatgg ctatactgct gccattggcc tattgggccc atgttgttaa
     2521 tcttatcaaa aatatccacg gttataaaaa attttggtta gtaattcgtt acctcaaatt
     2581 ctcagcaaat ctatactaaa tttgctatca atgcgtttgt tggttataaa atttattcac
     2641 atcatttgct aaatatctgt aatactgtgt attaacaaat tttatgatcg aatggcatga
     2701 attttttagt catgtaattc caaaaacatt aattttcaca aagattttga ataaatttgc
     2761 ctgcttaaaa caaagcatta aataataatt ggtttgatta tgaaaattca gggccttttt
     2821 aagtttgaaa gaattgtatt gctttccttt attattgttc ttttatcgtc gtgtagtgga
     2881 tttgaaaagg ttcttaagag caaggactat gagtataaat acaagatggc cttggagtac
     2941 tacaaaaaga aagatcatta ccgttatgta accctacttg agcagcttca aagcgtgtat
     3001 aagggtacat tccgtgccga caccattgat ttttatttgg ctcaaggtta ctaccaccag
     3061 ggcgactacc tgcttgcagg tcatcagttt gatatgttcc gtaaaaacta tcagcgcagc
     3121 gtttttaccg aggaggctga attcatgtat gcttactgtt actacatgtc gtcgccaagg
     3181 cctgagctgg atcaatccaa tacccttgcg gccattgagg cttttactga atttataaat
     3241 cgctacccca atacctccca taaggccgag gtgaataaga taatggttga attgcgcgat
     3301 aagctggttg aaaaatcgta ccttagcgca atgctttact acaagattgg cgattacaag
     3361 gcggctattg tggcaattcg aaatagtata agggactacc ctgaaagtaa gcaccgcgag
     3421 gagctaatgt ttcatctact taaagcatcg taccttttag ccgataatag tattgagagc
     3481 aagcgaaggg aacgttttca gaatgcgctt gatgagtact actcctttat cagtgagtac
     3541 ccaagttcaa agtactcgag cgaggcaaaa aaaatgtacg atgctactat gaaagtgttg
     3601 caaaatttaa actaagtata aatatcgcta tggacatcaa gaaaatcaat gcaccagtta
     3661 acacagtaac ccgcgatttg gataagttgg atagggaaac aggcaatatc taccaatcgg
     3721 ttcggataat tgccaaacgt gccaataaga ttagcattga tatgaaacag gaacttaacc
     3781 gtaagctcga ggaatttgct tactataccg ataaccttga agaggttttt gaaaaccgtg
     3841 agcaaattga gatttcaaag ttctttgagc gtcttcccaa acctacactc atagcagcac
     3901 aagagtttct ggatggaaag ctgcactatg agtttaagga agatcaaact tccgagagtg
     3961 aggcttaaag gaaaacatat tctaatcggt gttaccggaa gcattgcagc ctataaggct
     4021 gcaacgctta ttcgtttgct tgttaaggag ggtgccgagg ttaaagtgtt aatgacaccc
     4081 ctggccaagg agtttattac tccccttacg ttggcaactc tctcaaaaaa tcctattctg
     4141 gttgattttt tcaatcctga aaatggcgat tggaatagtc atgtggattt aggcctttgg
     4201 tccgatttat atatcattgc gccggcttcg gccaatacca ttgctaaaat ggctaccgga
     4261 gtagccgata atctgttgct tacaacatat ctttcggccc gttgcccggt tatggtagct
     4321 cccgcaatgg atttggatat gtataggcac ccagcaactc aacagaatat ctcaacactt
     4381 caggcgcgag ggaatattat tattgaagcg gcaacaggag agcttgctag cggcctgagt
     4441 gggaaaggta gaatggaaga acccgagaat attcttgagc aggttgtaag ttttttttta
     4501 accagtcagc gccttagtgg agttcgggta ttagtaactt cggggccaac ctacgagcat
     4561 attgatcccg ttcggtttat tggtaaccac tccagtggca agatgggtaa tgccattgcc
     4621 cttgagctgg ctcgacaggg tgctattgtt gattttgtaa gtggacctgt ttcgcaacac
     4681 cccaaccacc ccaatatcaa catacacagg gtaactagtg cagcagaaat gtacaaggca
     4741 gctaccaccc tttttggcga ggctaaggtt gccattcttg ctgctgccgt agccgatttc
     4801 acagttgaaa atgttgccaa tcaaaaaatt aagcgcgagg ccgatgacct tgttcttagg
     4861 cttaaaccca ccaacgatat tgctgcccac cttggtaaag taaagaagag caatcaactc
     4921 ctagttggtt ttgcactgga aacagctaac gagattgaga atgcaaaggc aaagctgcaa
     4981 cggaaaaatc tcgatatgat tgttctaaac tccatgaatg accctggtgc aggttttgga
     5041 accgatacca ataaggtaac ctttattatg cgcagtggtc aggtagttcc ctttgaaaca
     5101 aaacccaagg atatggttgc aatcgatatt gttgatgcag tatgcgaaat gttgaataaa
     5161 ggctgatttg catgaatagg tttgttggct tacttttagt attattaccc tttgtggcaa
     5221 actcgcagga actgcgatgc aatgtctccg taaactctca aaggattcag ggtaccaaca
     5281 ggctactatt ccagaacatg caaactgcca tttacgagtt catgaacaac actccgtgga
     5341 ccaaccatgt ttacggattt gatgagcgta ttgagtgtag catcatgctc aacataaccg
     5401 agcagatagg ctccgatgag tttaagggaa ccattcaggt acaatcacgt cgaccggtat
     5461 ttaatacatc gtacaacact acgatgctca attttcagga taataacctt cactttcggt
     5521 atcgcgagta cgagaagcta gagttttcag aatcatcgca cctcagcaac ttaacttcca
     5581 tccttgcgtt ttacgcatat atcattatag ggctggacta cgattcattt tcaaaactag
     5641 gaggaactga gtactttcag aaagcagaac gcattgtaaa taatgctcaa aatgctgttg
     5701 aacgaggctg gaaagcctat gagggaaaca gaaagaatag gtactggatg attgagaatc
     5761 tcctaaaccc aaagtatcaa cccttgcgcg aagcccttta tcgctaccac cgcttgggat
     5821 tagatgtgat gagcgaaaag gcagctgagg gaagggctga gattgccgaa tcgcttgctt
     5881 tgcttcagcg agtttaccgc gagaaacccg atccctatct tttcgccctg cagctgtttt
     5941 ttgatgctaa aaacgaggag cttgtcaata ttttttctga atcgtaccca accgaaaaag
     6001 ctcgtgtggt aaatatcctt acagaaattg acaaccccaa cgctgcaaag tataagcgta
     6061 taatagaaca gaatacacag taacaccgat aatagcatga ttcgtaaact ctacatacag
     6121 aactatgcta taattgatga gcttgaaata gagttcaagc aagggttaaa tataattacc
     6181 ggtgaaaccg gtgccggtaa atcgatattg ttaggtgcac tttccttgat acaaggccag
     6241 cgagccgata catcggtact caaacataac gataggtcgt gcattgttga ggttgagttt
     6301 gacatttcag agtataacct aaatgatttt tttgaggcaa acgatattga ttatgatgat
     6361 attaccacca ttcgtcgcca gataagtgag aatggtaaat cacgtgcatt tatcaacgag
     6421 attccggtta atcttaacca gctcaaggag cttaccgaaa agctcatcga tatccactcc
     6481 cagcatcaga accttttact gggtaacacc cgttttcagc tcgatgtgct tgatgccttt
     6541 gctgtatcaa gggatttact taccgattac cgtcagctgt acgatgagtt ccgaaagctt
     6601 aaaactgagc tcgataggtt agaggctaac gctgaaaatg caaagcgcga tttcgattac
     6661 ctatcccatc agctcaatga gctaaatgct gctaacctgc gtagcggcga actcgatgag
     6721 ctggaagcac ttcagaaaca acttacgcat gcatcggaaa ttaaaacagc tcttgagttc
     6781 tcctatggaa cccttaatgc cgacgaactt gcagttttat caaaagtaaa ggagattgag
     6841 tccaacctgc ggcgcattga atctgtattc tcccaggcta ataacctaat atctcggatt
     6901 gagagttgcc gtatagagct taaggatatt gccgatgaaa ttgacttgct caactcaaag
     6961 gttgaagttg acgatgaaca gttacatacc gtaacccaac gtatcgatct tatttactct
     7021 ttacttaaca agcaccgttt ggctaacttt gatgagctag ttgaattccg aaatcagctt
     7081 gaaagtagag taaatgaaat tgcacaaatt gacttcaacc tggatgaaaa gagaaaagcg
     7141 ctcaggatct tagagcaaaa gcttcaggaa aagtcaaaga accttagtca gattagagca
     7201 agctcagcgc ctaaggttga gcagttcgta acagaattgc ttcagcagct gggaatcaaa
     7261 cacgctgttt ttaggattcg tatcgagaaa ctggaagagt tccaaccctg gggtaccgat
     7321 agggtcacct tttacttttc agccaataag caaatcgagc cccaggaact ttcgagggtt
     7381 gcatcgggtg gcgagctttc caggttaatg cttagcctaa aatcgttact ggtaaaatca
     7441 accggtttac caaccataat ttttgatgag attgacacag gcgtttctgg cgagatagct
     7501 gataggatgg gaaccatcat atacgagttg gctaaaggta tgcaggtaat caatatcacc
     7561 cacttaccgc aaattgctgc aaagggcacc aaccacttct tggtttacaa gtcggtaacc
     7621 aacagccaaa gctctacagg tataaagctt cttacccccg atgaaagggt agtggaaata
     7681 gctaaaatgc taagtggaga gaaggttacc gatgctgcac tcatgaatgc aaaggaactg
     7741 cttaaatcaa acgttagttt aagctaattg aaccaatttc cggtttcttt gtttatcttt
     7801 gttagttcaa ttaaacgatg tatttatggc ttacaattta ctgaaaggga agaagggact
     7861 tatttttggt gccctaaacg aaaaatccat tgcgtggaaa gtggcagagc gtgcttttga
     7921 ggagggcgct gaggttgttt taaccaatac cccagtagca cttcgctttg gcaatgtata
     7981 tgagcttgct gagcgaatga acacggttgt tattcccgcc gatgccacta atattcaaga
     8041 cctggagaac cttattgacc aaaccatgga gcgttttggt ggtaaaattg attttgtgct
     8101 ccattccatc gggatgtcgc ctaacgttag gaagggcaga acttatgacg atttggatta
     8161 cgaatttttc cttaagacac tggatatttc agccatttca ttccataaaa ttttgcaaac
     8221 ctgctggaaa aaggatgcta ttacccatgg cggttcggta gtagcccttt cctatatcgc
     8281 agctcaacgt accctttacg gttacaacga tatggccgat gcaaaagccc tgcttgagtc
     8341 tattgcacgg agctttgggt atatctatgg cagggaacga ggtgttcgta tcaacacggt
     8401 atcgcagtca ccaactgaaa ccaccgcagg tggtggagta atgggttttg atgccctgct
     8461 cgatttctct gatcgtatgt cgcccttggg taatgccagt gcgctcgatt gtgccgattt
     8521 ttgtataacc ctattcagcg atttaacccg caaaatcacc atgcaaaaca tttataatga
     8581 tggaggattc tctagcatgg gtatgagcaa cagggccatg gctgtttaca accgaaacct
     8641 tgaggagtgc aaggactgta agtaatgtat ttggataaat aattgaaaag cccgccaatt
     8701 ggcgggcttt ttgttaacca agatacgatt ttagtaattt actacgtgaa gtgtggcgca
     8761 aacggcggat agccttttct tttatctgac gtacacgttc acgggttaag ccaaattttt
     8821 cgccaatttc ttcaagggtc atctcctggc aacctatccc gaagaaaagt ttaatgatgt
     8881 ccctttctcg ctctgtgagt gtggcaagag cgcgttctat ttcgcggctt aacgattcgt
     8941 ttattaaacc acggtcggcg cttggcgaat cgttgttgac aagaacgtcc aaaaggctat
     9001 tgtcctcacc atcaacaaag ggagcgtcaa ccgatacgtg gcggccactt gcgcggagcg
     9061 tatcatttac cttttccttg ggtagttcaa gtaaatcagc taactcctcg ggcgaaggtg
     9121 tacgttcaaa ctcctgctca aactttgaga atgctttgtt aattttgttt agcgatccta
     9181 cctggttaag cggtaggcga acaatgcgcg attgttcagc taatgcttgc agtatggatt
     9241 gacgaatcca ccaaacagca taggaaatga atttgaaacc acgggtttca tcaaattttt
     9301 cagcagcttt aataagccct aggttaccct cgttaattag gtcgggtagg ctaagtccct
     9361 ggttttggta ctgcttggct accgaaacca caaaacgcaa attggcacgt gtcaatttct
     9421 ccagcgctgc ctgatcgcct ttgcgaatac gctgggcaag ctcaacttcc tcttctacag
     9481 tgataaggtc ttccttacca atctcttgta aatacttatc gagcgatgcg ctctccctgt
     9541 tagtgattga ttttgtgatc tttaattgtc tcatccgacc cgtatattta atgcaaaatt
     9601 actacaaaca ctagttaata tcaagaaaaa aaatatgtta aacaaaataa acaagcaacg
     9661 ttctaacgtg ttaagttaaa acgttgctat ttttaaatag tttcatttaa tatgtcaaaa
     9721 tctcataatt tttagtttac gccaattgca tagtaggcaa ctacaccatt ggggtaaacc
     9781 ccctcaatta aaacaccacc cgatgaaatg cttagtacac gctttaagtc atcgatattg
     9841 cgaattggtg tccggttcat acgagtaata atgtatcctt tctttattcc gccttcctta
     9901 agtttaccat cctggagttc aactatttgc aaaccgtaat taattccaag ctcttgcttt
     9961 tgcttatcgg tgatttcttt aaactctgcc cctaagctac ttaacgtatc atcggttttt
    10021 actacaccca aaccaccttt aaggttgcgg agtgttacat tgaatcgctt ctttgaatta
    10081 tcgcgattta ccacaatgtc aacttgctga ttaggacggt aacggctgat ttgttcctgt
    10141 agctgagcag tgctgttaac atcaacgcca ttaaccgaga ggattacatc gccgggttta
    10201 ataccagcag catcagcggc gccacccttt tcaacatcaa atatgtaaac tcctttaatt
    10261 tcctttatac ctttttcctt ggctagctct gcggtaagtt cttggattct aacacccaga
    10321 atggcccgtt gtacctcacc aaattcaatg atatcggtca caaccttttt aacaatgttt
    10381 gagggtattg caaatgcata accagtaaaa gaacccgttc ttgaagcaat ggctgtatta
    10441 ataccaatga gttcacccct aaggttaatt aaagcaccgc cgctgtttcc ggggttaact
    10501 gcagcatcgg tttgaataaa cgattctatg gcaaattcgc gtccactaag tatgttgata
    10561 tttcgtgctt tggcacttac aattccggca gtaaccgttg atgttaggtt aaagggattt
    10621 ccaatggcta atacccattc gccaatttta atgttatcgg agttgccaaa ggataggaag
    10681 ggcagatcgg tagcatctat ctttagaagc gcaatatcgg tacttggatc agtccccact
    10741 agttttgcgg taaacttacg cttgtcgttc agcactacag taatttcatc ggcattttca
    10801 atcacatggt tattggtaac aatgaaacca tcgtgggtta aaataactcc agagccagag
    10861 ctctgaacct tttggcgcgg catatagtcg cgtggcccaa aaaagaagtc aaataagggg
    10921 ttcccgaacg agtagtcaac agattgggtg aactctgttg ttacgtgtac tactgcatca
    10981 accgatcgct ctgcagccca ggtaaagtca gtagaagagg aatcgggtgc aaacgctcta
    11041 actacctgag cgggagtttt ttgggtaaca actccctcat tattattcga actcaataca
    11101 tggttgctta aaatgatggc cagcatgcca ccaagagtgg cataaattac tggtagtata
    11161 aatttctgtg ctttcatagt ctaacttgtt ttatggtttc tgctaattta ggtaacaaaa
    11221 aaaatacctt gtgcagctga ttaacaaatg ttaaccagag gtatgtcaat ttttcatgtt
    11281 tttaaaataa agccacgaca aaattaaatg tgagctaaat cacattctga atttttggca
    11341 tcatgcgaaa tacttagtac gggtaaaagt taaagggtat atgaattatt tccgagaagg
    11401 tttcaccaac ctcaagcata tcctcatcaa tacttttgta ataccagttt acctgtacgg
    11461 ctttattttc ttttttaata ttttgtagta atgagagaat ttcaataatc tttttagagg
    11521 cagcagtgtt aaagtagtcg agcttgaaaa ccagattagt ttcttgattt ggctccttta
    11581 aatactcatg aaaccattca attataggct caaagaactc cttggggttc tcgggcataa
    11641 tttttccata gaactcaaac cggcccctct ccttatccag tgtgacctct ggggtatcgc
    11701 tggtttccaa caactttaat ggcttcatct ttttcaactt ttagtaaagt aacacaaagc
    11761 aaggactgtt aacaaaatta agaaaaaatg gaattaatgc aattttgttt tagtaattaa
    11821 ttttgccctc aaaaacttct tcggcaggcc cttcgagcca aacgttcagg tatttttctt
    11881 caatttgatc aaaatagacc ttgagtaaac cgccaagggc ttcaacctca attggggttt
    11941 ggttgatatc gaagtagtga gagtatgcta tggctgatgc tgttgcacca gttccgcagg
    12001 ctagggtttc agcctctaca ccccgctcgt aggttcggat ttttattcta ccaggagcta
    12061 cttcctgaat aaaattcaca ttggttcctc cattgttgaa attcacgctt tgccttatga
    12121 ggcggccctc gcggtccaca tcaacgccat taacatccct aaccatggtc acatagtgcg
    12181 gggagcctgt attgataaag taacagtcgt caattatttc tagggtttct acatcaatca
    12241 ttttgagctt tactgtgcca tcggtattga aaattgccga gtgcatccca tcaatccctt
    12301 gaaagtcgaa cgataatccg ttggccagcc caattttctt ggcaaaagtg gcaaagcatc
    12361 ttcccccatt cccgcacatg gtcgactcgt taccatcgca attaaagtaa cgcatgaatg
    12421 gcataccatt ctgggtttcg agcaatatca gaccgtcggc tccaattcca taatgtctgt
    12481 cgcaaaggtg tttaacacct tgaaccattg gtgtaaaagc ttggtcacga ttgtcaatca
    12541 ttataaagtc attaccagca ccatggtatt taaaaaactg tagcatcata aatcatgtaa
    12601 taatacccta caatattcgg gaaaaataac gaagaaccca ataaaattgg aaattaatag
    12661 gagaaaaatt aaaaattatt taggatcgat tttaaagcca accactagga tatcgtcaac
    12721 ctgcggatat tttgttccca accaatcctt taatgtgttt ttaagaatct cgcgctgctg
    12781 atcggggggc agaatggata tgccagccag aaggtcaata aaatttttaa tcatatattt
    12841 tcgtccatcg gggcccccaa attgatcgat aaacccatcg gagaacatat agaaccatgt
    12901 agggctttca accttaatta cgtgggtggt gaactcatcg ttacggtaat ccattccgcc
    12961 tccaattgat tctttatcgc ctctaatacg gttcacctcg ttgttaacca cataaaccaa
    13021 ggggttttta gctccagaga attcaacaat gttagttttg gtatcaacaa cgcaaagggc
    13081 catatccata ccatcacggt tatcggtttc atcctgcctg agagttcttc ggataccatt
    13141 gttaagttcg gttagaatgg ctcctggttt atgaattcca cggaatacaa tatcatcgag
    13201 caggttgtaa ccaatcatgg acaggaacgc accgggtaca ccgtgtccag tacagtcaat
    13261 tgccgaaacg gcaactttac ccgttttttc attggatccg tatccggtgg ttaactcctt
    13321 gaaccagtag tagtcgccac tcaccacatc gcgtggacgg aaaaatatga acgattcagg
    13381 gaaaatagcc tgtaggttgg attgtggagg gagtaaggcc ctttgaatgc cttgtgcgta
    13441 gttaatactt ttacttatgt tctcgttctg tttctggatt tggtcgcgtt gctgttgtat
    13501 ttcaatattt tgcaggttaa gcagttcgtt cgctttcttt ttgtctgtgt aattcttgta
    13561 gatggcaacc gatgagataa tcagtactaa aacaaatatt gcaccggcaa aaatgattat
    13621 cctttgaact ctggattgag cctctcgctc cctcaactgc gcctccttga gcattctatc
    13681 ggtattaagc agatcaattt cggtttgctt gcgttgggct tcctcctctg ccttgcgaag
    13741 gctatcctgc ttacgtagaa ctacaaagtt aagggagtct tgcatggctt ttgattgcaa
    13801 ctcgcgcaaa ctcataagga gttcctgttg agtgcgctgc atgcgttccc tttctgcttc
    13861 ggcctgcgat tttacaacct ttgtctcgta ttcagttttt atttgctggg tggcaagttg
    13921 ttgttcatag gcgttagcca tagcgccata ctcagttgct ttggccttgt ttcctaggtt
    13981 atcgtagttt tgggcaagca gtttaagcga gttaaggatt aagcggggat tgtttacttc
    14041 cctggaaagt tcaagtgcct cctcaagcag ttgaatggct ttttcggatt ggttaagggc
    14101 tgtgtgaatg tatgcaacat caattaaacc ggcagcaacc tctcctttat tattaagttt
    14161 tctgcgggct tcaaggctct tgtaaaagta ctcaagggta agatcaagcc tgtccatgtc
    14221 agagtatata agagctatgt tgctgtaaac agccttaata tcgttatagt ttccaacctt
    14281 ttcgttgagc ggaatggttt caagaaaata gttaatagcc tctttaggat taccaaactc
    14341 ccaataaata aatgctattt tattcagata aaatgctgct tgctgtgagt tgtttgcctg
    14401 cttgtatcga actactaact ccttgtatct atcaatatca tcctgttttt cttttgggat
    14461 tgtaacaatc tcctgctgac caactgcatg aatagcagtg gccatcacat aaaatagtaa
    14521 aaaaatcagt ttaatctgtt tcaacctaaa cttatatttt actcaaacat acttaatttt
    14581 tttcattgtt caaaaaacaa ccatgtattt tatttaatcc aaacatgagt taactaagtt
    14641 gctgattagc cttaggataa atattaactt tttaaaatgc cctttgctct gcttaagctg
    14701 tataaccatg ataaatgcaa tttttttaaa tacggttacc ttctatttat cgatattttt
    14761 aatattttta tggaaaatat tttgctttta tcatttattt ttgaaaattg gaaaatattt
    14821 aaaaggttaa aaaagtatgc ttaagcgaaa cctgttttac attattggcg ggttactatt
    14881 ttctaatctt gccttaggtc aagattttga ggttgcgccg gtgcgtttgg agtttgatgc
    14941 agaacccgct acaagccaga tgaaaactat taccgtaaag aatcatagca gtaggagagt
    15001 atcatatacc gtttcgttcg ctgattttct cccaacaaca agcggtgagc ggaaaatact
    15061 acctcctaat tcaaccaagc gcacctgtgc aaactggatt aacgttaacc cggctttttt
    15121 tgagttaaat ccgggcgagg atattcagat tcaggttaac atgctagttc ctggcgagga
    15181 attcggaact gcatggtgta tgctttttat acaaccaacc cgtgagcaaa ccagttggag
    15241 tgccgataag cagcttgctg caggaataac tgtaagcgga agaattggtg tattggttta
    15301 ccagtcgccc aagtcaaacc aaaactactc cattaaggtt agcaactttc aagaagtaac
    15361 caagccaggc gaccctcaaa gaaaattttc tgcagttatt gaaaaccttg gcgataaggt
    15421 tgctcactgt aaggttttcc ttttggcatc ggatatgcta actgcagagg aaaaacaatt
    15481 tcctccaaag gattttgaaa tttttcctaa gcttactagg actgtggaaa tggagttgcc
    15541 aaatgattta ccagcgggaa agtatgcttt ggctattatt gttgactatg gcccaaaata
    15601 cccactggaa ggatcgcaac ttgttattga tatagctggc gagattccta aagcagataa
    15661 aactgatagt acaaaagtaa atccataatt acccttgagg ttaaaagcca ttctatttgt
    15721 agtatttttt cagatggcgg gctgtttatt tgcccagtca acgtttgttt ttgttgaaag
    15781 gcaagctcca aatgggatgg ttaaagaaaa gtttcaggga gttgtagtta atggcgatac
    15841 cttacgacaa ggccgttacc tttacttttt tgataacgga gttttaaatc aagagggttt
    15901 ctatacaaac ggaaaacccg atagtgtttg gattacttac tacccaagtg gtgctaggtt
    15961 aagcctattc cattataaca gtggcaaaaa ggatggccct ttcctcttct ggaattccga
    16021 tggtacgctt taccagcagg ggtactacaa aaatgaccta ttggatggag tgcttgagac
    16081 ctattatcct aacggaaagc ttagcgcaaa atcagaatat agtaagggta ggctaaatgg
    16141 gattagtgag gttttcgacg agtccggatt actccgtctg cgtagtggat ataaaaacga
    16201 tacccttaac ggactctggg aaacctatta cgatactggc gagcttaact ataagggcac
    16261 taaatgtttg ggtaattact gcgatacgct ttacacatac tataaaaatg gagtattaat
    16321 gcgaaaggcc aactatagta atggcaggat agatggcgtt ttgatatcga attatgagaa
    16381 tggtgtattg cagattaagg cagagtatcg aaatggttct caggatggga attatactga
    16441 gtattacccc gatggtagta taaaagaaac cggaagctat ttaatgaacc aaaaggtggg
    16501 atggtggaca agctactatc agggtggaca aaagcatggc caaggcaatt tttcaaaagg
    16561 ggttttagat ggcgaatgga ttttttggtg gccaaacgga aatgtgaaac agcatggtag
    16621 ttactgtaag gggaaagctt gcggtaggtg gaaatatttt aacagtatgg gagcgcttat
    16681 tattgatggg aattacatag atgataaaga aaatgggatt tggacaacat tctatcccaa
    16741 tggagcaatt gccggtagga tatggtattt gagggggaaa ccggtatcgg taaagtggct
    16801 ttacgatggc caaggtttgc tgcagaaacg atttagcctt gaagggttag aaaaataaaa
    16861 ggccggaaat ccggcctttt ctactacgca tcgatatttg catatttggc atgacgttca
    16921 ataaactccc tcctaggggg aacctcatcg cccatgagca tggagaaaat cctatcagct
    16981 tcagcggcac tttcaatggt tacctgacgg agtattcggg tttcagggtt catggttgta
    17041 gtccaaagct gttcggcgtt catctcgccc aaacctttat agcgttgaac atgcactgat
    17101 ccatccttcc ccttaccaag ctcttcaact gctttcagcc gttcctcttc gttccagcaa
    17161 taacgctctt ccttaccctt ctttacaagg tatagaggag gtgttgcaat atagaggtga
    17221 cccttgttta tcaaatcggt catatagcgg aaaaagaaag tcataatgag tgttgcaatg
    17281 tggctaccat caacatcggc atcggtcatg ataataatct tgttgtaccg taaccctgaa
    17341 aggttaatgg ccttactatc ctcctcggtt ccaattgaca cacctaatgc agtaaaaata
    17401 ttcttaattt cttcgttttc gaatattttg tgttgcatgg ctttttccac gttcaggatt
    17461 ttaccacgta gcggtagaat tgcctggaaa cgcctatcgc gcccttgttt tgctgtacct
    17521 cctgccgaat caccctcaac aaggaaaatc tctgatacag cagggtcctt atcggagcag
    17581 tcggcaagtt tacccggaag gccagatcct gaaagaacgg ttttacgctg aaccagctca
    17641 cgtgctttac gtgctgcatg acgagcctga gcggcgagaa ttaccttttg aacaatgttc
    17701 cttgcgtcct tagggttttc ctccaggtag ttggccaagg cttcgcttac cgcctggtca
    17761 accgctattc gaacctcggg gtttcccagc ttggttttgg tttgaccctc aaattggggt
    17821 tcggccacct taacagagat aacggcggtt aatccctcgc gaaaatcgtc gccgctgatt
    17881 tcgaatttga gcttgctcaa cattcctgag tcgtcggcat atttttttag tgtgcgggta
    17941 agtgcacttc tgaatcccgt aaggtgggtg ccaccttcaa ttgtgttgat gttatttacg
    18001 tatgagtgaa cgttttcgga aaatgaagtg ttgtattgca tggcaacctc aacaggaact
    18061 tcacctttgt cgttctcaat gtaaataaca ttttcaatta aaggctcacg gtttatatcc
    18121 aaaaatttga tgaattcctt taaaccatcc tgtgagtaga attcagcgta acggtagcta
    18181 cgggtaccgt tttcaattat ctcgcgttta tcggtaagtg ttagcttaat gcctttatta
    18241 aggaatgcta gttctcttag ccgcgattcc agaatttcaa agttaaacac agtggtttga
    18301 gtgaatatct ggctatcggg catgaaggtt atagttgtac cggtttcctc agtgcttcca
    18361 ataaccttaa gctccgttgt tggcttaccg cgggaatact cctgcctgta tatctttcca
    18421 tcgcgtttga cctcagcaat taggtttact gagagtgcat taacgcacga tacacctaca
    18481 ccatgcaatc caccggaaac cttgtatgag tccttatcga acttaccacc ggcatgcaga
    18541 accgtaagca ccacctccag tgccgatttc ttttcctttt cgtggaagtc aaccggaatt
    18601 ccacgtccgt tatcggaaat agttattgac ccatcttcgt taataactaa atcgatgcga
    18661 ttacagtagc ctgcaagtgc ttcgtcaata gagttgtcaa ccacctcgta aaccaagtgg
    18721 tgtaatcctt tattaccagt gtcgccaata tacatggccg ggcgcatgcg caccgcctct
    18781 aatccttcca gaacttgaat gctactggct gagtaactag cactaccatt ttcacccttt
    18841 aattcttttt ccttatcgct catagcagaa tagcactaaa tcaactttaa atttaacctt
    18901 caaagataac caaaatattt ggtttatctt aatggttttg tatctttttt atttgatcaa
    18961 ctgattgata tttaattaat tacgcgtgat gttgatattt tttaaaattg atatcaaacc
    19021 ctacatattt gggtataagc agaagatgtc tttttgatta ttctggataa ttagcaatat
    19081 cactaaaatt tgtaacttac tccagccagc atattgaacc tctgggttgg gtatagatac
    19141 cagagctggt attttgatcc cgttatatta tttgcgtata caaaaatcga catcctttta
    19201 ttgtaaacat actcagcacc aaggttgata tccaggtatt ctttaagcct aattgggcta
    19261 tcgttagggt cttttgccca gcgcttacct acagcaaata cctcggcatt caatttcagc
    19321 ttattccgga atgcatactg gataaaaccg gttatttcat aatcgggcaa gtgccagggg
    19381 tgtgatatct tttgcgtttt gtactggtaa aatgctccat taagttttac tagaaacgat
    19441 tttgatggtt caaaggctat ttctccttta accgtagttc gctccgtatt ttcgtataat
    19501 aagctgaatc ggttaaataa ggggttattg ttatcggagt tggtgttaac cattagtccc
    19561 aaactatcaa taatggagta gttagcatta acatgatacg aaatggtagg gctgaattta
    19621 cccttaaccc ctccgcctac aatgagctta tatactgtat tccatgtatc aatgcttgag
    19681 gtcaaccatg ggttttgttt tagaagatga gagtatgagt tctcctgaac atagccatcg
    19741 atttcaacgt agggtataat gtaatggcta acaatatcgt acgagaggta agcgcgaggg
    19801 aagaagtagg tgttgtttac atttccattg gcatcgtaaa taatgtttaa accggcatag
    19861 gcatgccatt ggtcgccaaa gaaatgggcc cagggtttaa tctgcaaaag tgcgttattc
    19921 tttgaacctg ttggattgga gcgaccgtaa tgggtaaacg aaatttcgcc gccaaactgt
    19981 tcctgtccaa agtatttgtt gaggttaaag agtccagtaa ccattgtttc ctgaaggtcg
    20041 tacctatcgg ttaaatggta gaagttggtt tgagcatggt aatttatgct agctgaatct
    20101 ttgtttgtgg ttttaaattc aagatttgca gcaaacctat ttatgctttg cttgtcgttg
    20161 gaaggtgtaa gtgcaggatt ttcaaaaaag taaccatagt aagttcggtt attttgggta
    20221 aaaccgatgt cgcccagtaa aattttttgg ttaaacattc tcttaccaaa tgccatgata
    20281 tcgatatttg atgcaggggc tttaaccttt aggttgttgg taagttcaag tttggagcta
    20341 gtattaaaaa attttaccca agcaccgtaa aggtaatcct tgtttcggtt actgctgagg
    20401 taggcctcag cataggggag ggctgtgttc cctaaaccta cacgcatgta actcatatgt
    20461 aaatcgggca atggttcgga aaccattcgg gcagccgata ggggagtagg agtaaagtag
    20521 gtttcaatgg ggcgttgaat tatggagtaa gtaaagttgg ggtttacctt tatggtgtca
    20581 ataatcttgg gttgcagatt aatcttattt gcatcggata ttgatggttc ataggggcga
    20641 acaacctgaa cttccttttt tagatcgttg gtttgcgcag cggcatggct accagccatt
    20701 aaaaacattg ctgcagttag gggtattact atgtattttg tttcgcttct cattgctgtg
    20761 aaattaatct tgttttacag attgacgttc cttttcggat tttactaact ggtttagctt
    20821 tgaggtggcc tcgtcgatta ttccatcggt tgtgtacgag tagccatcta gaacgctttg
    20881 gagggtagct ttggcttgga aaaagtcatt ttgagcggca tacgagtcgg caagcaaaat
    20941 gaagcttttt gccaaccagt actggtgggg agtattattc tctgcaaacg agaaaacttc
    21001 gctttcagtt ctattgtact cgccgcgttc aaagtaaatt agagccttca tgaacttgga
    21061 ttcagcgccc tcaatagtct taaggttctg tgcaaccctt gaaaactcaa taagcgcatc
    21121 gtcgtacttt ttggaacgat agttggcttt ggctagttta aaacgggttt cacgctctag
    21181 ctcaggcgat agcttctcgg tgctcaatat gtttgccgca atcacgggta tttggtcatc
    21241 gcgttgcagc tcaaatgcac atcgtagctt gcctagacgg gcatcgagta acatgctctt
    21301 aacttcagct atcgactcta agttgtcaag gtaggtgtaa gctttttcgt actgtttttg
    21361 gttaaggtag atattgctaa tccttagtaa gctttgttca gtgaaactgt tacggggtct
    21421 attaataata ttttcaaagt attccactgc cttatcaaca ttgttattcc ggatattaca
    21481 gtcggcaatg tagtacattg catttactgc aaagttacct tgagggtttt cgctgaggta
    21541 gtcctcaaat gcaagtattg atttttcaca gttacctgta aggtaaactt tctccgctgc
    21601 catgtagctt agcgagtctt tctcggaagc acttatattg gcaatagatc caagtttgga
    21661 tgcatagtta aagtaggttg ctgcatcgcc tttatctata taaatgtttc taataccaat
    21721 tagggcattt ttggcttcag gtgaattcgg ataattttcc acaactctct tgtagtatgc
    21781 gagtgagttt tccaaatcgc ttgagttgta gtaaagcagc cnnnnnnnnn nnnnnnnnnn
    21841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21901 nnnnnnnnnn nnnnnnnnnn nctcttgtag tatgcgagtg agttttccaa atcgcttgag
    21961 ttgtagtaaa gcagcccaat ttgaactaaa gcttttgggt agtaactgct gttggggaat
    22021 tccgactcaa ctcgcttata gaaatcaatg gcctggcttg tattttcaag aatgttatat
    22081 gcctcagcta tctcaaaaat tgcatcatcg gtgtagcttg atgttgggta tttgctaaga
    22141 agccccttta atgtttctat ctttttctcc ggacggctaa ccaaacccaa tgaaaaacct
    22201 ctttggaata gggcgtagtc ggcatcgagc acatcaaggt tagccgcttt ttcatagtaa
    22261 tcaattgcag cccaatagcg tcgttggata aagaaacagt caccaatcct gttgtaagcg
    22321 tcggcaaccg tgtttactcg tgaatcgttt agtaagctat tatacttacg gaaccaagta
    22381 atggctgtat cgtactgttt cattttaaag tatgcatagc ccaggttata gtgggcaata
    22441 agatactctt ccagaccaaa agccccaggg gtaactataa acctttcaag gtcgttggct
    22501 gcgttttcaa agtttgatag gcggtaatgc gactcagctc tccagaatag cgcttgtgct
    22561 gcaatttgtt ggttataacc ggcatattca agcgattttg taaacatttg tatggcatcg
    22621 gtgtagttaa gattttggaa aagttcaagt ccgcggaagt atgctgcacg ctgcaacgca
    22681 gtatttatgg ttgcgtcttt cttactgatt ttttccaacg attccactgc ctccttgtag
    22741 tttcgtgaac ttaagtaggc aagtgttagg tagttataag cctcatcaat cctgggtgag
    22801 ctggggaact ctttaatgta tgccttaaag gcatcgattg cttcattgaa aggcgcgtaa
    22861 agctgttcgt atgttagctt agcgaagtta aatagtgcat cctccttgat aattctatca
    22921 aaatcaagtt ttgaggcaag gccaaatgct tggcgagcct tattcttatc gtcaagctta
    22981 aggtagcagt cggctaagtg gtagttagcg ttttggctaa gcgagtcgtc aatggtggca
    23041 atacgttcca agtactttgc tgcttcggaa tacttgctgt ttttgtagta ggcaaatccc
    23101 aaaaggtaaa tatcctcacg tgagagttgc tgtttgtttt tttcctcgta aatcttaagg
    23161 tagattagtg agctatcgta ctgtttaagc ttaaaaaagg catcgccaac aattttggca
    23221 acttcaggtg cacgtttgtt tttattattc ctaactaagg atggggaata caaggtaacc
    23281 tttttatatt cgccctgtag gtagtatata tgagtgatgt agtaaggaac aagttgagca
    23341 aaaagctcat cgttctgtag tttctcaaaa ccttttaggg ctgtagcgaa tttcttttga
    23401 atgtaagcaa tgtgtgagta atagtaggtt gctggtgctg caaacttatt atcgatatct
    23461 tttatctgga atagccatct attggccgat tcgtaatcgt tagtcatgaa atagccgtat
    23521 cctaacataa actgccactc atagcgagaa tctttgtcta ggccgttctt atcaattttt
    23581 gataaccagt acaccgcttg cgaatacttt ttttgttggt attgcatttt tcccatggca
    23641 aagtatgcct gatcaataag ctgactttca gggtagtttt gaataaattg ccctataaga
    23701 tattcagcat catcgttatt cagaaaaatt gcacacattg ccctgtagaa ctccgctgaa
    23761 accttttgct ggtagtaacc tttaggcaaa cctgcaatgt attgctcaaa tgttttttga
    23821 gcatcggcat acatttcctt ttggtatagg tcaagaccct tttcgtaggt caggtttaaa
    23881 gagttaaacc tactgctctc ctgcgctata agcgcaattt gaaaaaagaa aacgatgagg
    23941 taaaccagga gtaataacct tttcatcatt cgatataata tattaatcag gcctaaaaat
    24001 agtaactatt aacctaaaca tttattgttg agccaacttt taatcaacaa ttaaaataat
    24061 ttattaaaag ttgagtaaac tattaaacaa cggtattcta aaaagtgtta tatttacaga
    24121 tgtaacaacc tatacatata atggtaacag taaaagccat ctacaatccc aaacgaatga
    24181 ccgaaggagc tggggtaagt attcaaagga tattgggttt tgaaaccaga tacgagtgcg
    24241 atccctttct gctgctggac ttctttggtt tctcgtcgga atcggaatat aaggcaggct
    24301 tccctgagca tccccatagg gggtttgaaa ccattacata catgcttgaa ggccaagtaa
    24361 agcataccga tagcactgga ggtagtggaa taattggccc gggtgaggtt cagtggatga
    24421 ctgcaggaag tggcataatc catagcgaga tgccaacaag cgatagtgga aagatgtatg
    24481 gcatccagct ctggctaaat ctgccacagg agtataaaat ggttgatcct gtataccgtg
    24541 aactaaagcg ggaggagata cctgttgtag aaacaacctc ctctcaggtt aaagttattt
    24601 ccgggaagta tcgcaaacgc gatgggggat tgcagggcct ttacaagcct gttgatattt
    24661 ttgatatcaa gctttggccc aattccgcat tcgatgaagt tattactcct gagttttgga
    24721 attataagta ctttctgttt atttatgaag gagagtttac agttgcacat gatctaaaac
    24781 caataaaggc tccttgtggt gttgccctat cgcagggttc aataattcaa attagggcag
    24841 gtgaaagcgg agctaaattt ctgttctttg gcgccgagcc taatagtgag cccattgcct
    24901 ggcatggccc cattgtgatg aataccgact ccgaaatcca aaaagcgtta gaggaactca
    24961 gtaacggaac ttttttgaaa tagcatttat ggcagaacca cgaattgcat tagttgatac
    25021 caatgacaat attattgggt atggtgagaa acagcaagtt catttcgatg gaattctaca
    25081 cagagccttt tccattctaa tttgcaacgc aaagggtgag atgcttattc accaaagggc
//
LOCUS       DAOSAL010000031        24855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_1449_length_24855_cov_46.764113, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000031 DAOSAL010000000
VERSION     DAOSAL010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 24855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24855
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1449_length_24855_cov_46.764113"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="PK049_05985"
     CDS             complement(<1..762)
                     /locus_tag="PK049_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOK61142.1"
                     /translation="MLRDGFKISLSRFWAIFRSLGERRITLILSFVIGLLSGVAGVAL
                     KNTLHGVRDFVSGLVPMNTASLLYFGLPTIGILLTLLFVRYLIKDDLSHGVTKVLYSI
                     SRQGSKLKAHHMFSSIFGSALTIGFGGSVGAEAPVVLTGSAIGSNVGSFFRMNYKIRT
                     LLIGCGAAGAIASIFKAPIAGIVFTLEVLMLDLTTASIVPLLISAVTASSVSYLLMGR
                     NVVFAYDVVRPFALHNIPWFILLGIFCGLVSLYFIR"
     gene            885..>1828
                     /locus_tag="PK049_05990"
     CDS             885..>1828
                     /locus_tag="PK049_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOK61143.1"
                     /translation="MDFVKYKLDNGLVLIVHTDYSTPIAAFNLLYSVGSKNEDPERTG
                     FAHLFEHLMFGGSANIPVFDEPLERVGGENNAFTSNDLTNYYITVPTENIETAFWLES
                     DRMLSLAFSQKSLDIQKSVVLEEFNQRYLNQPYGDIWLYLRPLAYKVHPYRWPTIGQS
                     PEHISRATLADVKNFFFSHYAPNNAILCVAGPVDPNEILELTHKWFGSIEPRKLANKS
                     IPREPEQTEPRFLTLEREVPFNAIYKAYHMCNRMHPDYPAVDLLSDLLSNGRSSRLYQ
                     RLVKEKQLFSSVNAYITGDVDEGLFILTGQLYNSTTFE"
     assembly_gap    1829..1928
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1929..3009
                     /locus_tag="PK049_05995"
     CDS             <1929..3009
                     /locus_tag="PK049_05995"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOK61144.1"
                     /translation="SRTSQQIAEELDFYGSFLEPSFSRDFASVTFYTIGKHFYRSLDV
                     LADILTNPTYPQHELELFCKKGKQSLLVDLEKVSTLSRQRFFSALFGSNHPYGSFATP
                     NDYDVLSQTDLVQFRNRYHKASNGLIVIAGKVDAKQVDFILNGFTNPIFEQCGEIELG
                     LPSFTTTDYRVFSYKKDAVQAALRIGRVLPKRNHPDMPGLVVLNTILGGYFGSRLMRN
                     IREDKGFTYGISSFIIPMAELSALVVSTEVGSAFAESTVDEVFKEMLKLQTEPVSNDE
                     LELVRGYMTGQVLRTFDGPFAVADSLASLFQYNNLDFEYINNLIRTINEITPTQLLGL
                     AKKYLDVDRMVVSIAGSKRIRGYKE"
     gene            3015..4628
                     /locus_tag="PK049_06000"
     CDS             3015..4628
                     /locus_tag="PK049_06000"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOK61145.1"
                     /translation="MKNIRQVILMVGLTFYMPTLWAQFSDKPNPPMLHYISNNLDNGD
                     VTIFWTKPPHEPQIADPLGYIIYVPRNPIDPDADGWDPIATVDQNTFQYTHIGANGNG
                     QRVYYRVALLGASEPSQMTVKHSQVWITASYDSCNAKIDLSWIGYEAWPLNASAPYRN
                     IRYQLYMGHDLNWNTYNQIGDYDGYSNRHSVTNVQENQDYYFYIKAIRMDKGYESYSN
                     LYLIHTQMARRPTYVSVDSVIATNNGNRIYYSIDPNSRVIDYKLWRWEQPDTAQSIFS
                     AKTIEGFDVPTKSMSIDTNDTWAARTRTFYYKVDAYNGCNDAVLVSNLCNTIIPRLSP
                     KGNVVSIQWDPLILDTQRKPDRAGNRIEYSIYRRSFTVNDDISGPGNLERIANGLSVT
                     EYADDLSGFKGQSPLYRIRFNYYIEALELSPANDTVTLSRSREISTEILPGVTMPTAI
                     APNDPRSENGHERSLFKPVIDFEANFILTIYDRWGGVVYHGNQGWDGRNSRGEYVKEG
                     TYVYRLVVSTADAGDIIKTGSISVVYPKK"
     gene            4644..6881
                     /locus_tag="PK049_06005"
     CDS             4644..6881
                     /locus_tag="PK049_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HOK61146.1"
                     /translation="MSLQTDEEKLLIQSHFEDLLNSCTRCNSPEDKELIVKAFNLANE
                     AHKGVRRKSGEPYILHPIAVAKIVTSEIGLGAKAVACALMHDVVEDTDYTIEDIERIF
                     GKKIASIIDGLTKITDVFDANSTIQAENFRKILLTLADDIRVILIKLADRLHNMRTLE
                     SLPATKQMKIASETIYLYAPLAHRLGLYAIKTELEDLSLKYRYPQVYEEIRSKIADNE
                     KRRVQLINHFSLPIIQKLEENNIDFEISGRPKSVYSVWKKMQTKNVSFEEIYDLLAVR
                     IVFNPYPHIPEKTQCWHIYSLITDIYKPKPDRLRDWVSTPKANGYEALHCTVMGPDGK
                     WVEVQIRSRRMDENAERGFAAHWRYKEMDATSQENELDKWIRRVREMLENPQENALEF
                     LDEFKLNLFTSEIIVFTPKGETRSLPKGATALDFAYEIHTEVGNRAIGAKVNHRLVPL
                     SYQLNTGDQVEIITAASQKPTWELLNFATTAKAKAAIKNALKGEVKNRIEKGQQLLEE
                     KLLEMGIQPSSRVFKKILPAYEVSSKDELYAKIGSGLINLDNLKKILKKNTQSKWIKY
                     WKLQLGFGAKKVEPESEIQIKYDLKKPIVLGADTDSDNPSYLVANCCNPIPGDEVIGY
                     LSPDDIVIVHKATCAEAVRLMAQHGDKIVAAKWTTQKALSFLARVEVNGMDRIGIISE
                     LTKVISDELMVNIRKFHIESHDGIFEGFIDLYVHDVTHLNNLILNIMKVKGMENVRRM
                     DKFDD"
     gene            complement(6978..7367)
                     /locus_tag="PK049_06010"
     CDS             complement(6978..7367)
                     /locus_tag="PK049_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOK61147.1"
                     /translation="MKIAVPTRHGHVDEHFGHCEFYTVYTIENGKVTAKETLGSPQGC
                     GCKSNIAFDLQQMGVEIMLAGGIGSGAINVLAAHGIQVVRGCSGNTDEVVDLYINGEL
                     TDSGESCSHHEHHHGSHQGEHGHSCNH"
     gene            complement(7406..7735)
                     /locus_tag="PK049_06015"
     CDS             complement(7406..7735)
                     /locus_tag="PK049_06015"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HOK61148.1"
                     /translation="MPRSKCPRRICHSPDIHLFKPAGKPLHLMEIVVLEADELEAIRL
                     ADGIGLYHEEAAKQMGVSRQTFGRILEQARSKVAKALTNGMALRINKSQTEIFCDQQN
                     NQNSEHI"
     gene            8449..8922
                     /locus_tag="PK049_06020"
     CDS             8449..8922
                     /locus_tag="PK049_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOK61149.1"
                     /translation="MTCSDTKLVVKRILTDYLERKGHRKTPERFAILDEIYSQESHFD
                     IETLYIKMKNKNYQVSRATLYNTIDLLLECGLVIKHQFGKNSSLYEKAYECKQHDHLI
                     CSRCGKVIEFCDPRIQEIINSAQKNSGFTISHHSLYLYGLCKDCQTEIDKVDLSL"
     gene            9056..10324
                     /locus_tag="PK049_06025"
     CDS             9056..10324
                     /locus_tag="PK049_06025"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOK61150.1"
                     /translation="MSKVDILLGLQWGDEGKGKVVDVLTPNYSVIARFQGGPNAGHSL
                     EFNNIKHVLHTIPSGVFHPGCLNLIGNGVVIDPIILAEEIHSLEKLGVNLKKSLKISK
                     KAHLILPSHRELDAASEAAKGKGKIGSTLKGIGPTYMDKTGRNGLRVGDILLPDFKDR
                     YNFLKNKHKEILRQYNYSSNIEQHEEEWFKGIELLRQYDLIDSEYEINSYIKQGKRVL
                     AEGAQGSLLDIDFGSYPFVTSSNTICAGACTGLGIAPHAVGEVYGIFKAYCTRVGSGP
                     FPTELDNEIGEKMRKIGVEFGATTGRPRRCGWLDLVALKYAIMLNGVTQLIMTKPDVL
                     DSFETIKVAVAYKVNGIETDRLPYDINTPIEPVYKEFKGWMTDISGFKSDSDLPDELM
                     AYIQFIEQETGVPVRIISVGPNRDETIVRE"
     gene            complement(10332..10886)
                     /locus_tag="PK049_06030"
     CDS             complement(10332..10886)
                     /locus_tag="PK049_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61151.1"
                     /translation="MEMQTVDESTTLISTIVSFTAIQDQISNTLSINFPKAQEVIINR
                     SKARITDSKGNVIWKGSLEKKVTSENTLNFKFGKDWLNKKGAQPYTIEWETNVLVSSP
                     TGNYVWPACLSGNINSVKYLGLRVFADNEFREKIETNLEVSSRLMDQSSGLSGVVWEL
                     SNYRTKKAWQYTKPIENPFVKISF"
     gene            complement(11020..12417)
                     /locus_tag="PK049_06035"
     CDS             complement(11020..12417)
                     /locus_tag="PK049_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOK61152.1"
                     /translation="MEKIRQTLRDHAWARWTALAVVAFTMLTGYYLTDVMAPLKGLLE
                     QQLSWDSSEYGLFTSAYGWFNVFLFMLIIGGIILDKMGVRFTGLMAATIMVIGTSIKF
                     WAVSTHMLDGQVWNILWIFPIKAQVFMAALGYAIFGVGVEVAGITVSKIIVKWFKGKE
                     LALAMGLEMATARLGTALALATSVPIAKAFGHVSKPILFCLILLVIGLISFIIYTFMD
                     KKLDASQAEYDKSNNVTNEEEAFKISDIFNIITNKGWWYIAILCVLFYSAVFPFLKYA
                     ADLMVNKFGVDESLAGTIPAMLPFGTILLTPFFGNIYDRKGKGATIMLIGSLLLIFVH
                     TMFSIPFLNHWIIAIVLIIILGIGFSLVPSAMWPSVPKIIPENQLGTAYSLIFWVQNW
                     GLMGVPALIGWVLDKYCITGQKVVEGVTVNTYNYTIPMIIFTSLGILALVFALLLKAE
                     DKKKGYGLELPNIQK"
     assembly_gap    12498..12597
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12817..13740)
                     /locus_tag="PK049_06040"
     CDS             complement(12817..13740)
                     /locus_tag="PK049_06040"
                     /inference="COORDINATES: protein motif:HMM:NF024747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HOK61153.1"
                     /translation="MDENKIFDLSCCTLCPHECGIDRYAHTGFCNVGVNPVVSSIFPH
                     KGEEPVISGEKGICNVFFAHCNLQCIYCQNHQISRNTKHSKKWLTDNKVIVDEIKSIL
                     NTGIGMVGFVSPSHQVAQMVDIVNSLNKSGYKPIIVYNSNGYDKVETLQMLEGIVDVY
                     LPDLKYYDSILAQRFSGVSDYFQLASKAIKEMYRQKGSTLILNNEGLAEFGMIIRHLV
                     LPGHANDSIKLLNFLAEEVSTKLYISLMSQYYPPAELTLPNELNQHVGIEEYKKVIEV
                     LENHSFRGWVQDPVSSHFYRPDFESNNPFSE"
     gene            13839..>15679
                     /locus_tag="PK049_06045"
     CDS             13839..>15679
                     /locus_tag="PK049_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005638066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOK61154.1"
                     /translation="MMKTIIDLFEESVSKFSDNPYIWEKVTDRYEPTSYAQTRTEVYR
                     LAAGLLSLGVKKDDKIALLSEGRKLWVISELGILYTGAVNVPLSVKLDGNDLLFRLVH
                     SETSMIFVSGQQASKVEKLRSQLPNVKRVIYLDQPEKLEGDDLFIGDVFKLGNEFLKN
                     NPGKVEEVSKSIKPADYANISYTSGTTADPKGIILSHRNYTANVEQALTLMDIPVHYK
                     TLLILPLDHCFAHVAGLYSFMAKGASIATVQVGKTPMDTLKNIPINIKELKPNLLLSV
                     PALAKNFKKNIENGIRAKGKFTEMLFNHALKISYKYNKEGWNKGTGFTFIYKPLIWLY
                     DKILFSKIREVFGGELDFFIGGGALLDIELQRFYYAIGIPMLQGYGLSEATPIISSNS
                     LKRHKLGSSGHLVKYLELKICDENGNELPVGEKGEIVVKGENVMVGYWKNEKATAETV
                     RDGWLYTGDMGYMDKDGFLYVLGRFKSLLIASDGEKYSPEGIEESLVQHSKFIDSVVL
                     HNNQKPYTTALIVPSKEALKKAIVAKGIDPESHEAAKEAITIINNEIARFKPGGEFGD
                     MFPERWLPTTFAILDEPFTEQNGLVNSTMKVVRGKVEKLYENRLEFM"
     assembly_gap    15680..15779
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15780..16042
                     /locus_tag="PK049_06050"
     CDS             <15780..16042
                     /locus_tag="PK049_06050"
                     /inference="COORDINATES: protein motif:HMM:NF017913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HOK61155.1"
                     /translation="ETIKGFLRDEILHVELVVLVAIIAISRKVIVWGISKTTSQEMFS
                     LAAMLAALALTYWVIKTCYKKKYNKLGSNLPKANSSCGDVNV"
     gene            16134..16943
                     /locus_tag="PK049_06055"
     CDS             16134..16943
                     /locus_tag="PK049_06055"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOK61156.1"
                     /translation="MRKGALGILMNYLHFRKKCLPKGGYGIHSPFVFDLYTTTIENDS
                     RESVFESIEVYRKELLGCDIGIIRKSIAESLNGGFRGELVKLSRIARTASVPPHLGRL
                     LYRIVNRFKPNVMLELGTSVGISSMYIAKANPHGVLYTIEGDEAVFGIAQKRFDTMNI
                     TNIKPICGIFETELPSIAREVPGFDFVFIDGDHNGTKLLQYFNVILPKLNENSVVVVD
                     DIRWSNDMEDAWRELKANRQVSVSIDLFRCGVLFFRKGIAKQNFMLRYGPF"
     gene            16969..17700
                     /locus_tag="PK049_06060"
     CDS             16969..17700
                     /locus_tag="PK049_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005808801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK61157.1"
                     /translation="MSEHYLINITNIKKYYQVGSVVVKALKGVDLTIKRNEYVAIMGP
                     SGSGKSTLMNILGCLDTPTDGTYILNGTDVSKLSDDRLAEIRNKEIGFVFQTFNLLPR
                     YTALDNVMLPLIYAGIPKHERIEMAKLALKNVGLDDRMEHKPNELSGGQRQRVAVARA
                     LVNSPSIILADEPTGNLDSKTSIDIMNLFEDIYEQGNTIIVVTHEEDIARHARRIVRL
                     RDGHIESDEPNPNPILRQKVVITEQ"
     gene            17778..18326
                     /locus_tag="PK049_06065"
     CDS             17778..18326
                     /locus_tag="PK049_06065"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOK61158.1"
                     /translation="MKIYTKTGDKGSTSLIGGTRVPKYHPRIETYGTVDELIAFTGLL
                     RDQDIDRHTVETLIFIQDRLMNCASILAADCTDCKVRIPKIESADIQRLENEIDAMDA
                     QLEPLTSFVLPGGHPVVSVCHVCRTVCRRAERLVTHLAEETPLPENLVVFLNRLSDYF
                     FTLSRKLGKDFGVEQIKWQPNL"
     gene            18406..18627
                     /locus_tag="PK049_06070"
     CDS             18406..18627
                     /locus_tag="PK049_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HOK61159.1"
                     /translation="MYWTLELASKLEDAPWPATKEELIDYAIRSGAPLEVIENLQELE
                     DDNEIYESIEDIWPDYPTKEDFLFNEDEY"
     gene            complement(18660..>20172)
                     /locus_tag="PK049_06075"
     CDS             complement(18660..>20172)
                     /locus_tag="PK049_06075"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HOK61160.1"
                     /translation="RIRFEGAKSMKNLNINGQKIIRYIGNVRFRHENTRIVCDSAYHN
                     PNDNWFDAFGNVSIQQGSTRITGQTLHFDGKTSVGKITGKEVKLIDDETTLTTQTLYF
                     NSKTNVAYYTTPGEITSKESKLTSQRGYFNRSSNLLSFAGNVVMLDPDGTIYTDSLSY
                     NTKNELAYFFGPTHIFNEDNFAYCEKGWYNKKIEQTNLQQNAYILSQENKIFGNNIFY
                     DKSIKFAHAIGNVVLVDTLNRAYIYGQKANFWQETEQAEVTENPYLMLIDNSDSLFLK
                     ANYFKVITQKHENSTDSSYRVLKALGNVAFYRHDVQGFCDSMIYNTRDSAIDMYVDPI
                     LWNDVNQMTADHITIHSKNNKINQMDFNGNAFITSFEQDKFYNQIKGKTITAFFSNGK
                     AEKIDVNGNGQAVYFIRDGNTLTLANRSESARISIMVKDNKASRVTFHEKPSSNLYPI
                     EKVELEDVMLKGFKWLEAKRPANRYSVIPKDFKEQPEERGNKKQREILSSKNK"
     assembly_gap    20173..20272
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<20273..21240)
                     /locus_tag="PK049_06080"
     CDS             complement(<20273..21240)
                     /locus_tag="PK049_06080"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOK61161.1"
                     /translation="MRAFSLPTLFLLIFSFSFSSHAQENISIDKVIAVVGNERILLSD
                     VEQELLRIKMQGSTNANIDRCQLLEQLLIQKLLITQAKIDSIEVNEGSVESEVDRRLK
                     YFINQIGSEKALENYFNKPIFEIKDDLREIIREQQLTQQMQRKIVDKVKITPSDVKAF
                     YKKIPSDSLPDIPMQYELQQIAIYPPDAEKAKLEAKEKLLEIRNRILNGERFSTLAAL
                     YSEDRASAIKGGELGFRSREELVKAFADAAFNLKEGQISQVVETEYGLHIIQMIERRN
                     DQVNVRHILLKPQFTTDLLSKAVQKLDSIATLIRNDSITFENAALR"
     gene            complement(21248..22087)
                     /locus_tag="PK049_06085"
     CDS             complement(21248..22087)
                     /locus_tag="PK049_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOK61162.1"
                     /translation="MNRLLFSLIVVTGFLTACQNADNSRPVARVYDKYLYESDLQNIF
                     LTNVSKEDSILIAKAYIEKWIQTQLLLKQAENNLTDKEKNVARQLEDYRASLLIHKYE
                     QAYVNQKLDTLVKETEVEQYYYSNKDNFVLSEPIVKALFIKLRKEAPQTPKIKEIYRS
                     KKQEDIQLLDNLAYQAAIFYDFFNDQWIPFSVITRQLPYTIDNIKETFSRQYIEMEDG
                     SFIYLVSLREVKEQGETAPLAYITNEIKQLIINARKQNLIDQLERNLFIKAKDDRNFE
                     IYK"
     gene            complement(22092..24059)
                     /locus_tag="PK049_06090"
     CDS             complement(22092..24059)
                     /locus_tag="PK049_06090"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOK61163.1"
                     /translation="MKKVFRVAIITAGVLAGQIAYAQKQGSETLLTINNTPISADEFI
                     SLYIKNTKATGAKIDKSTLDDYLNLFINFKLKVQAARDAGIDTLYSFRNEYNGYVDQL
                     AQSYLIDQETLDGLTREAYQRMLEEVSASHILISIPETVTGSDTLKYYQKAIEARSKV
                     LSGEPFGKVALTTSNDPSVSRNNGYLGWFSAFQMVYPFESAAYNTPVDSVSMPVRTRF
                     GYHIIKVHGRRPAKGQIKVAHIMVRVNPNASPQEMENARQKIVDIHNRLLNGENFAEL
                     AKKESQDNSTAPNGGELPWIRSGQIIPEFEEVAFALKDIGDISKPFQTSFGWHVVKLL
                     DRKLPGEFEEMLPEIKSLMARDSRSMLIRSSFIKKLKKEYGVEEFPAESAKHLSSLID
                     SSIFKGNWKAPNIKTNPTLLVVKGLNYTLSDLLQFIGQNQQKSKGNSLQSFISSIYTD
                     WVNQTIINFEKSLLPQKYPEFKQLANEYLEGMLLFEISNREVWTKASDSTALNTFFEN
                     HRDKYLWGEKIYYTVYQSADSKIAQALVKSLKKNPAINPDELVKKWNKKAQNVTYNQQ
                     VGFPDNPLLSGYTQWNSGINSSVQNNNHLVVHISKITTGEPKELNDCRAEVMADYQNY
                     LEESWIQTLKEKYKVDINNDTYSKLLENLNK"
     gene            complement(24103..>24855)
                     /locus_tag="PK049_06095"
     CDS             complement(24103..>24855)
                     /locus_tag="PK049_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOK61164.1"
                     /translation="DVDAIVIDTAHGHSKGVIEMLKMVKKEFPKLEVIVGNIATADAA
                     KMLVDAGADAVKVGIGPGSICTTRVVAGVGVPQLNAIWDVASAIQGSGVPVIADGGIR
                     YSGDIVKALAAGADTIMAGSLFAGVEESPGETIIYNGRKFKSYRGMGSLDAMQQGSKD
                     RYFQDVEDDIKKLVPEGIVARVPYKGTLAEVFYQLIGGLRAGMGYCGAPNLDALKKAK
                     FVRITHAGIIESHPHDVAITREAPNYSRDTSY"
BASE COUNT         7030 a         5093 c         5212 g         7120 t
ORIGIN      
        1 tctgataaag taaagtgaca ccaaaccgca gaatatacca agcagtataa accaggggat
       61 attgtggagt gcaaatggcc gaacaacatc ataggcaaac accacattcc gccccatcag
      121 caaatagcta accgacgatg ccgtaacggc cgaaattagc agcggcacaa tggaagcggt
      181 ggtaaggtcg agcattagta cttcaagggt gaacacaatg ccagcaatgg gtgccttaaa
      241 aattgatgct atggcacctg ctgcgccgca tccaattagc agtgttcgga ttttgtaatt
      301 catcctgaaa aagctcccaa cgtttgaacc aattgctgac cctgttagca ctactggagc
      361 ctcagctccc actgaacctc caaacccaat ggtaagggca ctgccaaaaa tggaactaaa
      421 catatggtgt gcctttagct tgctaccctg tcgcgaaatg gaatacaaaa ccttggttac
      481 tccgtggctt aaatcgtctt taatgaggta tcgaacaaat agtagggtta gtaaaatccc
      541 tattgtaggc aaaccaaagt aaagcaagct agcggtattc ataggcacta atccgctaac
      601 aaaatcccta accccgtgaa gcgtattttt tagtgcaaca ccggcaacac cgcttaaaag
      661 tccaataaca aaacttaaaa tcagtgtgat tcttcgttcg cctaagctgc gaaaaattgc
      721 ccagaacctt gataacgata ttttaaaacc atccctaagc atgatgctaa tttaaaaaaa
      781 gtttagcaat gcctcaaaat atcgacattg cttttaaatc tctaatcatt atacctaaaa
      841 catcaacaaa aatagttaat attgaggata tttttgtgag tatgatcgat tttgtgaaat
      901 ataagctaga taatgggtta gtgctaattg ttcacactga ttatagcact cccattgcag
      961 cctttaactt gctttacagc gtgggttcaa aaaacgagga cccggaacga acaggctttg
     1021 cccatctgtt tgagcattta atgtttggtg gttcggccaa catacctgta ttcgatgaac
     1081 cgcttgagag ggtggggggg gagaataatg catttacctc caacgattta actaattact
     1141 atatcaccgt tcctactgaa aacatcgaaa cagccttttg gcttgaaagc gacagaatgc
     1201 ttagccttgc cttttcgcag aaaagcctcg atatacaaaa aagtgtggtg ctggaagagt
     1261 tcaaccagcg atacctaaac caaccttacg gtgatatttg gttgtacctt cggcctttgg
     1321 cctacaaggt tcacccatac cgttggccaa ccatcggaca gtcgcccgag catataagcc
     1381 gtgccacatt agccgatgta aaaaatttct tctttagcca ttatgcccca aacaacgcaa
     1441 tactttgtgt ggcaggccct gttgacccca atgaaattct ggagcttacc cataaatggt
     1501 ttggttccat tgaaccacgt aaacttgcaa ataaaagcat tccaagggaa cctgagcaaa
     1561 ccgaaccccg tttccttacc ctggagcgcg aagttccttt caacgccatt tataaggcct
     1621 accacatgtg taaccgtatg caccccgatt accctgcagt ggacttactc tccgatttgc
     1681 tatcaaacgg tcgatcgtcg cgtttatacc agcgactggt gaaggaaaaa cagcttttca
     1741 gctcagtaaa tgcttacatt acaggcgacg tagatgaggg attattcatt ctaaccggac
     1801 agctctacaa ttctacaacc tttgaggann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnta gtagaacatc gcagcaaatt gccgaggagt tagattttta tggaagtttt
     1981 cttgagccct ctttctcacg cgattttgca tcggtaacct tttacaccat tggtaaacac
     2041 ttctaccgct cattggatgt tttggctgat attttaacta atcctactta cccgcaacat
     2101 gagctggaac ttttctgcaa aaaggggaaa caatcgttat tggtcgattt ggaaaaggta
     2161 tctaccctct cacgccaacg atttttcagt gctcttttcg gtagcaacca tccctatggc
     2221 tcttttgcta cccctaacga ttacgatgtg ctatcacaaa ccgatttagt tcagttccgt
     2281 aatcgttatc ataaagcctc aaatggtttg attgttattg ctggcaaggt agatgctaaa
     2341 caggtcgatt ttatactaaa tggttttacc aaccccattt ttgaacaatg cggtgaaatt
     2401 gagttagggt taccctcatt taccacaacc gattacagag ttttttcgta taaaaaagat
     2461 gcagttcaag cagctttgcg aataggcagg gttttaccca aacgcaatca ccccgatatg
     2521 cccggtttag ttgttcttaa taccatactg ggtgggtact ttggctctcg cctgatgcgc
     2581 aatattaggg aagacaaagg gtttacatac ggaatatcct cgttcattat tccaatggct
     2641 gaactgtcgg cgctggttgt ttcaaccgag gttggttcag cctttgccga aagtacggta
     2701 gatgaggtat ttaaagaaat gcttaaattg cagacagaac cagtaagcaa cgatgaactg
     2761 gaacttgtta ggggctacat gactggtcaa gtacttagaa ctttcgatgg cccatttgct
     2821 gtagccgata gccttgcaag ccttttccag tataataacc ttgattttga gtatatcaat
     2881 aatctgatca gaacaatcaa tgaaattaca cccacacaac ttcttgggct ggcaaaaaaa
     2941 tacttggatg ttgacaggat ggtagttagc attgccggaa gcaaaagaat tagagggtac
     3001 aaagagtaat gtgtatgaaa aatatacgac aagtaatttt gatggttggc ttaacctttt
     3061 atatgccaac actttgggcg cagtttagcg ataaaccaaa tccgccaatg ctccattata
     3121 tcagtaacaa tctggataat ggggatgtaa cgatattttg gactaaacca ccccatgagc
     3181 cccaaattgc cgatccattg ggttatatta tttacgtccc ccgtaatccc attgaccccg
     3241 atgccgacgg ctgggatccc atcgctacgg ttgaccaaaa tacattccag tacacccata
     3301 ttggtgccaa tgggaatgga caaagggttt actaccgagt ggcattgctc ggagcctctg
     3361 aacccagtca gatgacagtt aaacactcgc aggtttggat taccgcaagc tacgatagct
     3421 gtaatgccaa aatcgaccta tcgtggattg gttacgaggc ttggccttta aatgcctcag
     3481 caccttaccg caacatccgc tatcagcttt acatggggca cgaccttaac tggaatacct
     3541 ataatcaaat tggcgattac gatggctata gcaacaggca cagcgtgaca aatgttcagg
     3601 aaaaccagga ttattacttt tacatcaaag caattcgaat ggataaaggt tatgagagtt
     3661 actccaacct atacctgatc catacccaaa tggcccgtag gccaacctat gtcagtgtcg
     3721 atagcgttat agcaaccaac aatggtaata gaatttacta ctccatcgat cccaatagcc
     3781 gcgttatcga ttacaaactc tggcgatggg aacagcccga tacggctcag agtattttca
     3841 gcgcaaaaac tattgaaggg tttgatgttc ccaccaaaag catgagcatt gataccaacg
     3901 atacatgggc agcccgcacc cgcacgtttt actataaagt tgatgcctat aatggctgca
     3961 acgatgccgt tttggtttca aatctctgca ataccatcat acctcgccta agccccaagg
     4021 gcaatgtggt cagtatccag tgggatcctt taatcctcga tacccaacgt aaacccgata
     4081 gggctggcaa taggattgag tactccatct ataggcgttc ctttaccgtt aacgatgata
     4141 tttctggccc aggcaattta gagcgaattg ccaacgggct gtcggttacc gaatatgccg
     4201 atgatttatc agggtttaaa ggacaaagcc cactttaccg gataaggttc aactactaca
     4261 ttgaggcatt agagctctcg cctgctaacg atacggtaac tcttagccgg tcgcgtgaaa
     4321 tatcgacaga aattctaccc ggggtaacta tgcctacagc cattgccccg aatgatcccc
     4381 gaagcgaaaa tggccatgag cgtagccttt tcaagccggt tatcgatttt gaagctaact
     4441 ttatcttaac catatacgac cgctggggcg gtgtggttta ccatggtaat cagggttggg
     4501 acggacgcaa tagccgtggt gagtatgtta aagagggaac ttacgtttac cgtttagtgg
     4561 ttagtactgc cgatgctggt gatataatta aaaccggaag catctccgtg gtttacccca
     4621 aaaaatagct aattggaaat agcatgagtt tgcaaaccga cgaggaaaaa cttcttatac
     4681 aatcgcattt tgaggatttg cttaatagct gtacccggtg caactcacct gaggataaag
     4741 agctaattgt taaggctttt aatcttgcca acgaggcgca caagggcgtt cgccgaaaat
     4801 cgggcgaacc ctatatactg catcccattg cggtagcaaa aattgtaacc agcgagattg
     4861 gactgggtgc aaaggctgtt gcctgtgccc ttatgcacga tgtagttgag gataccgatt
     4921 atactattga ggatatcgaa agaatttttg ggaagaaaat cgcctccatc atcgatggat
     4981 taaccaagat taccgatgtt tttgatgcaa actcaaccat tcaggccgag aatttcagga
     5041 aaattctttt aacccttgcc gacgatattc gggtaatact tattaagctg gccgataggt
     5101 tacataacat gcgaactctt gagtcgctgc cggctaccaa gcaaatgaaa attgcgagcg
     5161 agaccatcta cctttatgca ccattggctc accgtttagg actctacgcc attaaaacag
     5221 aacttgagga cctaagttta aagtatcgtt accctcaagt atacgaagag ataaggagta
     5281 aaattgccga caacgagaag cgaagggtgc aacttatcaa tcacttctcg ttaccaatta
     5341 ttcagaaact tgaagagaat aacatcgatt ttgagatcag tggaagacca aaatcagttt
     5401 actccgtttg gaaaaaaatg caaaccaaga acgtttcgtt cgaggagata tacgatttac
     5461 ttgcagtccg catagttttt aatccctatc cacatatacc tgaaaaaacc cagtgctggc
     5521 acatttactc gctgattact gatatttaca agccaaagcc cgacaggttg cgcgattggg
     5581 ttagcacccc aaaggctaac gggtacgaag ccctgcactg taccgtgatg ggacctgacg
     5641 ggaagtgggt tgaggtccaa attcgctcac gccgaatgga cgaaaatgca gaacgaggtt
     5701 ttgctgcgca ctggcggtac aaggaaatgg atgccaccag ccaggaaaat gaactcgata
     5761 aatggattcg acgcgttagg gagatgctgg aaaatccaca agaaaatgca cttgagttcc
     5821 tcgacgagtt taagctaaac ctgtttacct ccgagattat tgtatttact ccaaaaggcg
     5881 aaacccgtag cttgcctaag ggagcaaccg ccctcgattt tgcctacgaa attcatactg
     5941 aggttgggaa tagagctata ggtgccaagg ttaaccatag gctggttcca ttaagctacc
     6001 agctcaacac gggcgaccag gtagaaataa tcactgcagc ctcacaaaaa ccaacatggg
     6061 aattgctcaa ttttgctact acggccaaag ctaaagcagc cattaaaaac gcccttaaag
     6121 gtgaagtaaa gaataggata gagaaggggc agcaattgct tgaggaaaaa cttttagaga
     6181 tgggtattca accctcatcg cgtgtattca agaaaattct accagcttat gaggtttcat
     6241 caaaggatga gctttatgcc aaaattggtt cagggcttat caacctcgat aacctgaaaa
     6301 agattctgaa aaaaaatacc caaagcaagt ggattaagta ctggaagttg cagctaggct
     6361 ttggcgctaa aaaggtagag cccgaaagcg aaatccaaat aaagtacgac ttaaaaaagc
     6421 ccatagtgct gggcgcggac actgactccg acaacccgtc atatcttgta gcaaattgct
     6481 gcaacccaat tccaggcgac gaggttattg ggtacctctc gcccgatgat attgtaattg
     6541 ttcacaaggc aacctgtgct gaggctgtta ggctcatggc tcaacatggc gataagattg
     6601 ttgctgcaaa gtggaccacc cagaaagccc tatcgttcct tgcccgtgtt gaggttaacg
     6661 gaatggaccg aatcgggatt atcagtgagc ttaccaaagt gatttccgat gagctaatgg
     6721 tgaacattcg caaattccat atcgaaagcc acgatggcat ttttgagggc tttattgacc
     6781 tatacgtgca tgatgttact caccttaaca acctgatact aaatattatg aaggttaagg
     6841 gaatggagaa cgttcggcga atggacaagt ttgatgatta aacccggatt acacaccatt
     6901 gcaatgtgca acccgggtta atgtttattc ctcaggtatt tctattaatc aaccgcaatg
     6961 agtttattca aagcgtgtta atggttacac gagtgaccat gttcgccttg gtgtgagcca
     7021 tgatgatgtt catgatggct acagctttct cctgagtccg ttaactcgcc attgatgtag
     7081 agatcaacaa cctcatcggt atttcctgag caacccctaa ctacctgaat gccatgagct
     7141 gcaagcacat taatagctcc tgaacctatt cctccggcaa gcattatttc aacacccatt
     7201 tgttgtaaat caaatgcaat gttcgattta cagccacagc cctggggcga tccaagtgtt
     7261 tcctttgctg ttactttgcc gttctcaatg gtgtaaaccg tgtagaactc acaatgacca
     7321 aaatgttcat caacatggcc atgtctggtt ggtactgcaa ttttcataat catttttttt
     7381 gttagtaaac cacttattct ctatatcaaa tgtgttctga attctggtta ttttgctgat
     7441 cgcagaatat ttcagtttgg cttttgttga ttctaagagc cataccgttt gttagcgctt
     7501 ttgccacctt actccttgcc tgctcaagta tccgcccaaa ggtttgcctt gataccccca
     7561 tttgttttgc cgcttcttcg tgatacaaac ctataccgtc ggcaagcctt attgcctcaa
     7621 gttcatcagc ctctaaaaca acaatttcca tcaagtgtaa aggtttaccg gcaggcttga
     7681 ataaatgaat atcaggggaa tggcagattc ttcgtggaca cttgcttctt ggcatatgct
     7741 caaaatattt ttcacaaatg taagcatatg cccaaaatga tagctataat taatgttaaa
     7801 attttaggca aggcctatta ttaacaaaca actactacca ttgtcatccc cagcggagtg
     7861 aaggatctct aatagacctc aatgagatgt ttcgctagcg ctcaacatga caacattatt
     7921 acaagtagtt ttcaacgaat aaccatcctt atcatgctga gctaacccaa gcatctcctt
     7981 acctttaatc cttgtttttt tggaaatcgc tatttaccat acttttcttt tgcgatgcaa
     8041 cgtttctgtg ttactctttg gaaaatgtat tgcgtgcatc gctatacatg tgggaataat
     8101 catggtgtta caaatattac gcccctacgg ggcttaagtc gttgttcgtg aaactattct
     8161 tttaactttt aacttttaac tagttctaac ttcctctaac tccatcatat tccatctatt
     8221 ccgactcatt ccgactcaaa gtcacgcgtt cttaacggtt cacgtttaac ggactagaac
     8281 ttcctctaac ttccgcctat tccgtctcat tccgattcat tcttcaagaa atgagcccgg
     8341 ttaattcatg gcacttcgga taaacttttt ctatgtttta ctgttagatg ttgtataaca
     8401 aaaattttat cctatttttg ctatttaaag taagtctaaa tttaagctat gacctgcagc
     8461 gatactaaac ttgtagtaaa acgcatactc accgattacc tggagcgtaa agggcacagg
     8521 aaaactcccg aacgtttcgc cattcttgac gagatttact ctcaggagtc gcactttgat
     8581 atagaaacgc tctatatcaa aatgaaaaat aaaaactacc aggtaagtag ggcgacgctc
     8641 tacaacacta tcgacttgct actggagtgt gggctagtaa taaaacacca gtttggtaaa
     8701 aattcatcgc tatacgagaa agcttatgaa tgtaagcaac acgatcacct tatttgtagt
     8761 cgctgtggta aagtaattga gttttgcgac ccccgaatac aggaaataat taactccgcc
     8821 caaaagaata gtggattcac tatttcgcat cactcgcttt acctttatgg cctttgtaaa
     8881 gattgtcaaa ccgaaataga taaggttgat ctttcccttt agattgttaa tatcttcctt
     8941 ttcaaaattg tgctaaaagc attaccttta tatgcttttt ggccttaaaa tttgtgtgaa
     9001 caattggttt cgctaaggat tttatttgcc aaaagcagca taattattaa aggagatgag
     9061 taaagttgat attctattag gcctccagtg gggcgacgaa ggtaaaggga aagttgttga
     9121 tgttctaact cccaattaca gtgtaattgc acgattccaa ggtggtccaa acgcaggtca
     9181 ttccttggag tttaataaca ttaaacatgt gttgcatacc attccttcag gggtattcca
     9241 tcctggatgc ttaaacttaa ttggcaatgg tgtagtaatt gacccaatta tcctggctga
     9301 ggaaatacac tcgcttgaga agcttggggt aaacctgaaa aaatcgctca aaatttccaa
     9361 aaaggctcac ctcattttac cctcgcaccg tgagctcgat gcagcatcgg aggcagcaaa
     9421 gggaaaagga aagataggtt caaccctgaa aggaattggc cctacctaca tggataaaac
     9481 cggacgtaat gggctacgtg tgggcgatat cctcttaccc gattttaagg atagatacaa
     9541 tttcctaaag aacaaacaca aggaaatatt aaggcaatat aactattcaa gcaacattga
     9601 gcagcatgag gaggagtggt ttaagggcat agagcttcta cgtcagtacg accttatcga
     9661 ctctgagtac gaaataaata gctacatcaa acagggaaaa cgggtgcttg cggaaggtgc
     9721 tcagggatca ttgctcgata tcgattttgg ttcttacccg ttcgtaacct catcgaacac
     9781 catttgtgcc ggcgcttgca cggggttggg tattgctccc catgcggtgg gtgaggttta
     9841 tggcatattc aaggcatact gtacccgagt aggctctgga ccattcccca ccgagcttga
     9901 taacgagatt ggcgaaaaga tgcgaaagat aggcgttgag tttggtgcca ccactggtag
     9961 gccgcgtcgt tgtggttggc tcgacctagt tgcattgaag tatgccatta tgctcaatgg
    10021 tgtaacccag ctaataatga ctaaacccga tgtgcttgat tcctttgaaa ccatcaaggt
    10081 tgcggtagca tacaaggtaa acggtattga aaccgatagg ttgccctacg atattaatac
    10141 gcccattgag ccggtttaca aggagtttaa gggttggatg accgatattt caggcttcaa
    10201 atctgatagt gatttgcctg acgagctaat ggcttacatc cagtttattg agcaggaaac
    10261 aggcgttccg gttaggatta tttctgttgg accaaacagg gacgaaacaa ttgtaaggga
    10321 atgaaaaaca gttagaaaga tatttttaca aagggatttt caataggctt ggtatattgc
    10381 caggcctttt ttgtacgata gttgctcaac tcccaaacta caccgctaag acctgaggat
    10441 tgatccatta accttgagct tacctcaagg ttggtttcaa ttttttctct aaactcgttg
    10501 tcggcaaaaa ctcgtaatcc gaggtacttt actgagttaa tgtttcctga aaggcaggca
    10561 ggccaaacat agtttccggt aggcgaactt accaaaacat tcgtttccca ctcaatggtg
    10621 tatggttgag cacctttttt attcagccag tctttaccaa acttaaagtt aagcgtgttt
    10681 tccgatgtaa cttttttctc aaggctacct ttccaaataa cattaccctt tgagtcggta
    10741 attcttgctt tacttctatt tataataact tcctgcgctt ttgggaagtt aatggatagt
    10801 gtattggata tctgatcttg aatagctgta aacgagacta ttgttgaaat aagtgttgtg
    10861 ctctcgtcta cggtttgcat ctccattgca aacctatcaa tctctatttg tgtaccagaa
    10921 atttcttgag agaaactttg ttgtgaaaaa gttaataaac aggcaaaagc aaataaagag
    10981 gacatccgcg atgcggacat cctctttaaa aatgaaaaat tacttttgaa tatttggcag
    11041 ttcaagtcca taaccctttt tcttatcttc agccttaagc agtaatgcaa aaactagggc
    11101 aagtatccca agtgaggtga aaataatcat tggtatagtg tagttgtagg tattaactgt
    11161 aacgccttca accacttttt gacctgttat acagtactta tcgagcaccc aaccaataag
    11221 ggcaggaaca cccattaagc cccagttttg tacccagaaa attaaggagt aggcagttcc
    11281 aagctgattc tccgggataa tttttggaac tgaaggccac attgccgagg gaaccaatga
    11341 gaatccaata cccaaaataa taataagtac aattgcaata atccaatggt taaggaatgg
    11401 gattgagaac atggtgtgca caaagataag cagaagtgag ccaatcagca tgatagttgc
    11461 ccctttgcct ttcctgtcgt aaatatttcc gaaaaaggga gtaagcagta tggtaccaaa
    11521 gggcaacatg gcaggtattg tgccggcaag cgattcatca acaccaaact tgtttaccat
    11581 taagtcggcg gcatacttaa ggaatgggaa tacggctgag tagaatagta cacaaagtat
    11641 ggcaatatac caccaaccct tgttggtaat gatattgaaa atatccgata tcttaaatgc
    11701 ttcctcctcg ttggtcacat tgttactctt gtcgtattcg gcttgggaag catcgagctt
    11761 cttatccata aaagtgtaaa tgataaatga aattaagcca attacgagaa gaatcaagca
    11821 gaaaaggatg ggctttgaaa catggccaaa agccttagca atgggtaccg atgtagccag
    11881 agcaagtgca gtccccaaac gggcagtagc catttccaaa cccatagcaa gcgctagctc
    11941 cttgcccttg aaccatttta caatgatttt tgaaacggta ataccggcca cttcaacacc
    12001 tacaccaaaa atggcatacc ccaatgcagc cataaacact tgtgctttaa tggggaaaat
    12061 ccatagaata ttccatacct gcccatcgag catatgggtg gaaacagccc agaacttaat
    12121 ggatgtacca ataaccatta tggtggcagc cattaggccg gtaaaccgaa cacccatttt
    12181 gtcgaggata ataccgccaa tgattagcat gaaaagaaat acattaaacc acccgtaggc
    12241 gctggtaaaa agtccgtatt ctgaactatc ccatgagagt tgttgttcta aaagtccttt
    12301 gagcggggcc atcacatcgg ttaagtagta accggttagc atggtaaaag ctacaactgc
    12361 cagggctgtc caacgtgccc aggcatgatc gcgcagtgtt tgtcgaatct tttccattta
    12421 gtttaaaaat taggtttagt ttgttttgtg cagcaaataa aatcaaaata gctcaaacca
    12481 aatacatttt ttgtcaannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngct
    12601 ttttgcaggg caatggggtc aacctttttg tcttttttaa agtacttgca acctgtgcca
    12661 acaaatgctg ccagtactat aaacagtata attcttttca tagggtttct cggttaaggt
    12721 ttaatttcag tgcaatatat atacaaattt tcaataaatc aaaatttagc gctaaaagtt
    12781 caagcaaccg tgctgatttt atccctttca ggtatcctat tctgaaaagg gattgttact
    12841 ttcaaaatcg ggacggtaaa aatggctgct taccgggtct tgaacccagc ccctgaagct
    12901 atgattttcc aatacttcaa taactttttt atattcttca atacccacat gctggtttaa
    12961 ttcattgggc agtgtaagct ctgcaggggg gtaatactgt gacatgagtg agatgtatag
    13021 ttttgttgaa acctcttcgg caagaaagtt gagaagttta atgctgtcgt tggcatgacc
    13081 tggcagaact aaatgcctga taatcattcc aaactctgct aaaccctcgt tgtttaggat
    13141 tagggttgac cccttttgcc tatacatttc cttaatggct tttgaggcta actgaaaata
    13201 atccgatacc cctgaaaacc tttgcgctaa tatgctgtcg taatatttta aatcgggtaa
    13261 gtaaacgtca actataccct caagcatttg tagggtttca accttatcgt atccattgga
    13321 gttgtataca ataattggct tatagcccga tttattcaag gaatttacaa tatcaaccat
    13381 ctgggcaact tggtgcgacg gtgaaacaaa gccaaccatt ccaataccag tgttgagtat
    13441 gctttttatc tcatctacaa ttaccttatt atcggttaac cattttttgc tatgcttggt
    13501 attcctactt atttggtggt tttggcagta aatgcattgc aggttacaat gcgcaaaaaa
    13561 aacattacat attccttttt cccctgatat aactggctcc tcacctttgt gaggaaaaat
    13621 tgatgataca actggattta ccccaacatt acagaaaccg gtatgagcat acctatcaat
    13681 gccacattca tgggggcaga gggtgcagca gcttaaatca aagattttat tttcatccat
    13741 gccacaaaaa tactaaacct actactttta gatatgatta ggtgagttgc aaaacaaaat
    13801 tttatagttt tgtctatgct aatggtgaaa attttgaaat gatgaaaaca ataatcgatc
    13861 tttttgagga gagcgtaagt aagttttccg acaaccctta catttgggag aaagtaaccg
    13921 ataggtacga accaacatcg tatgcccaaa cacggactga ggtttaccgc ttagctgcag
    13981 ggctattaag ccttggagta aagaaagacg acaagattgc gttactgagc gaaggacgca
    14041 agctttgggt aataagtgaa ttggggatac tctatactgg tgctgtaaat gttcctcttt
    14101 cggtaaagct agatggtaat gacttactat tccgattggt tcattccgaa acatctatga
    14161 tttttgtatc gggacagcaa gcatcaaagg ttgaaaagtt acgctctcag ctacctaacg
    14221 ttaaaagggt aatatacctc gatcagccgg aaaagttaga gggcgacgat ctgtttattg
    14281 gcgatgtttt taagctaggc aatgaatttc ttaagaataa tcctggaaaa gttgaggagg
    14341 tttcaaaaag cattaaacct gccgattatg ccaatatcag ctatacatca ggcaccacag
    14401 ccgacccaaa aggcataatt ctttcgcaca ggaactacac tgcgaacgtt gagcaggcct
    14461 taacccttat ggatatacct gttcattata aaacgttgct aattttaccg ctcgatcact
    14521 gctttgcgca tgtggccggt ttatactcat ttatggctaa gggagcaagt atagctaccg
    14581 ttcaggttgg caagaccccc atggatactt tgaaaaatat ccccataaac atcaaggaac
    14641 ttaaaccaaa ccttttactc agtgtgcccg ctttagccaa gaacttcaaa aagaatattg
    14701 aaaacggcat tagagctaag ggaaaattta ctgagatgct ttttaaccat gccttaaaaa
    14761 tctcatacaa gtacaataag gagggttgga ataagggaac cggttttact ttcatttata
    14821 agccccttat ctggctttac gataaaattt tgttcagcaa aatacgtgag gtttttggtg
    14881 gcgagctcga ttttttcatt ggtggtgggg cattacttga tattgagcta caacgctttt
    14941 actatgccat tggaattcca atgctacagg gctatggatt atcggaagca actcccatta
    15001 tcagttcaaa ctcattaaaa cgccataaac tgggttcatc gggtcacctg gttaaatacc
    15061 ttgaacttaa aatttgcgat gagaatggca atgaactacc agtaggggaa aaaggcgaaa
    15121 ttgttgtcaa gggcgagaac gtaatggtgg gttactggaa gaacgagaag gcaaccgccg
    15181 aaacggtgcg cgatggatgg ctttacactg gcgatatggg atacatggac aaggatggct
    15241 tcctttacgt tttaggaaga ttcaaaagct tgcttattgc cagcgatggc gaaaagtata
    15301 gccccgaagg aattgaagag tcgttggttc agcactccaa gtttattgat agcgttgtgc
    15361 ttcacaacaa ccaaaagcct tacaccacag ccctaattgt tccctcaaag gaggctttaa
    15421 aaaaagccat agttgctaag ggaatcgatc ctgaatcgca cgaggcagcc aaggaggcca
    15481 ttaccattat aaacaacgag atagcccgct ttaaacccgg aggcgagttt ggcgatatgt
    15541 tccctgaacg atggctaccc actacctttg ccattcttga cgaacccttt actgagcaga
    15601 acggtttagt taatagcaca atgaaggtgg taagaggaaa ggttgaaaaa ctttatgaaa
    15661 atcgtcttga gttcatgtan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
    15781 ggaaaccata aagggttttc tcagggatga gatactccat gtggagcttg tggttttggt
    15841 tgccataatt gctatctcgc gcaaggttat agtatgggga atttcaaaaa ccacatcgca
    15901 ggagatgttt tccctggctg ccatgctggc tgccctggca ttaacctatt gggttattaa
    15961 aacttgctat aaaaagaagt ataacaagtt agggtcaaac cttccaaagg caaattctag
    16021 ttgtggcgat gttaatgttt gataatatta ggtataataa acttttttta acacatccag
    16081 cggaggttta aattaccctg gggcttacct tagcatttca tagaattgcg aaaatgagaa
    16141 agggagcctt ggggattttg atgaattacc ttcatttcag gaagaaatgc ttaccgaagg
    16201 ggggatatgg catacattca ccttttgttt ttgatctgta taccactaca attgaaaacg
    16261 atagcaggga atcggtcttt gagagtattg aagtgtaccg caaggagctg ttaggctgcg
    16321 atattggaat tattcgtaaa tcgattgccg aaagcctgaa tgggggattt agaggggaac
    16381 tggttaagct aagtagaatt gcccgaactg cttcagtacc accacacctg gggaggttgc
    16441 tatacaggat tgtaaatcgc tttaaaccca atgtaatgct cgagcttgga acatcggttg
    16501 gtattagctc aatgtacatt gccaaggcaa acccacacgg agttctttat accattgaag
    16561 gcgatgaagc agtttttgga attgcccaaa aacgtttcga taccatgaat attacaaata
    16621 ttaaacccat ttgtggtata tttgaaacag aattgccaag tattgcaagg gaagtacccg
    16681 gtttcgattt tgtttttatt gacggcgacc ataacgggac taaacttttg cagtatttca
    16741 acgtcatact tccaaagtta aatgaaaatt cagtggttgt ggttgacgat attcgctgga
    16801 gtaacgatat ggaagatgcc tggagggaac tcaaggccaa caggcaagtg tcagtttcca
    16861 ttgacctgtt taggtgtgga gtgcttttct tcaggaaagg catagccaaa caaaatttta
    16921 tgcttcgata cggaccattc taacaagtaa aatataataa cctagtatat gagcgagcat
    16981 tacttaatca acattacaaa cattaaaaag tactaccagg taggaagcgt tgttgtgaaa
    17041 gccttaaaag gggtcgacct aaccataaaa cgaaatgagt atgtagcaat tatggggcct
    17101 tcgggttctg gaaaatcaac cctgatgaac attctgggtt gcttggatac accaaccgat
    17161 ggaacctata tactaaatgg aactgatgta agcaagttgt ccgacgaccg gttagccgaa
    17221 atccgtaata aggaaattgg gtttgttttc caaaccttca acctcttacc ccgttacact
    17281 gcactcgata atgtaatgct accgctaatt tatgccggaa taccaaaaca tgagcgtatt
    17341 gaaatggcaa agttagccct taagaatgtt gggcttgacg accgaatgga acataaacca
    17401 aacgagctct ctggcggtca gcgtcagcgc gttgccgtgg cacgagccct ggtaaatagc
    17461 ccttctatca ttcttgccga tgagcctact ggtaacctcg actcaaaaac gtctatcgat
    17521 attatgaacc ttttcgagga tatctatgag caaggcaata ccataattgt tgttactcac
    17581 gaggaggata ttgcccgcca tgcccggaga attgttaggc tccgcgatgg tcatattgag
    17641 tctgatgaac caaatccaaa ccccattctc agacaaaaag ttgtaattac tgaacaataa
    17701 atattggttt gcgttgattt aaatatttct tgtttaaaca aggtagataa actatattgc
    17761 aacgcaacaa ttattgtatg aaaatttaca caaaaacagg cgataagggc tcaacatcgc
    17821 tgataggcgg aactcgcgtt cccaagtacc atccacggat tgaaacctat ggcacagtag
    17881 atgagctgat tgcatttacc ggtttgctcc gcgaccagga tattgataga cacactgttg
    17941 aaaccctcat atttattcag gacaggttaa tgaactgtgc atcgatactt gctgcggact
    18001 gcaccgattg caaggtaagg atacctaaaa ttgaaagcgc cgatatacag cgtctggaga
    18061 atgagattga tgccatggat gctcagctag agcctttaac atcgtttgtg ctacctggag
    18121 gtcatccggt tgtttcggtt tgccatgttt gccgaaccgt ttgccgcagg gctgagcgtt
    18181 tggttactca cctagctgaa gaaacaccac tccctgaaaa tcttgtagtt ttccttaacc
    18241 ggcttagcga ttactttttt accctttcac ggaaacttgg caaggatttt ggggtggaac
    18301 aaataaaatg gcaacctaat ttgtaattcc aaaaaattta tatatttgtg aggttgtcag
    18361 ttgttatatt gaactgtcaa tcagtttttt aaaaccaaaa acactatgta ttggacatta
    18421 gaattagcat ccaagttaga agatgctcca tggccagcca caaaagagga attaattgat
    18481 tacgccattc gttctggtgc tccccttgag gtaattgaaa atcttcagga gctggaggat
    18541 gataatgaaa tttacgaaag tattgaagat atctggcccg actatcccac caaagaggat
    18601 tttctattca acgaggatga gtactaaaaa ttaaggagag gtcaacctct cctttttgct
    18661 tatttatttt tactgctgag tatttcgcgt tgctttttat taccacgttc ctcaggttgc
    18721 tctttaaaat ccttaggtat tacgctgtac ctgttagccg gtcgtttagc ctcgagccat
    18781 ttgaaaccct taagcataac atcttccagt tcaaccttct caatggggta aaggtttgat
    18841 gatggtttct cgtggaaagt tacccggctt gctttattat cctttaccat aattgaaatt
    18901 cttgcgctct cggagcggtt ggctaaggta agggtattgc catcgcggat aaagtaaacc
    18961 gcttgtccat ttccatttac atcaatcttt tcggctttgc cattactaaa aaatgcggtt
    19021 atggttttac ctttaatttg gttgtaaaat ttgtcttgct caaatgaggt aataaaggca
    19081 ttgccgttaa aatccatttg gtttattttg ttattttttg aatgaatggt aatgtggtcg
    19141 gcggtcattt ggtttacatc attccataga ataggatcaa catacatatc aattgctgag
    19201 tcgcgggtat tgtatatcat tgagtcgcaa aatccttgta catcgtgcct gtaaaatgca
    19261 acattgccta atgctttaag aactctatag gatgagtccg ttgagttttc atgcttttgg
    19321 gtaattactt taaagtagtt tgctttaagg aataaactat cggaattatc aattagcatc
    19381 aggtaggggt tctccgtaac ctcggcttgc tcagtttcct gccaaaaatt ggctttttgg
    19441 ccatagatgt aagccctgtt taaggtatca actagcacta catttccaat ggcatgggca
    19501 aacttaatag atttgtcgta aaaaatgttg ttgccaaata ttttattctc ctggctaagt
    19561 atgtaagcat tttgctgtag attggtttgc tcaatttttt tattgtacca acccttttcg
    19621 cagtaggcaa aattatcctc attaaagata tgggttggac caaagaagta agctagttca
    19681 ttcttggtgt tgtagctaag ggagtcggtg tatattgttc cgtcaggatc caacatcact
    19741 acatttccag caaacgatag gaggttagaa gagcggttaa agtagcccct ttggctggtt
    19801 agcttcgact cctttgaggt aatttcaccc ggagttgtgt agtaggcaac attagttttt
    19861 gaattaaagt atagggtttg ggtagtgaga gtggtttcgt catcaataag cttaacctct
    19921 ttgccggtaa tcttacctac tgaggttttg ccatcaaagt gaagggtttg cccggttatt
    19981 cgggttgaac cctgctgaat gctaacatta ccaaaggcat caaaccagtt atcattggga
    20041 ttatggtaag cgctgtcgca aactatacgg gtattctcat gccgaaagcg aacattccct
    20101 atatacctga taattttctg gccattaata ttgagatttt tcatgctctt tgccccttca
    20161 aacctaattc ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaaacggag
    20281 tgctgcgttc tcaaaggtaa tactatcgtt gcggataagc gtggcaatgc tgtcgagctt
    20341 ttgaactgct tttgaaagca aatcggttgt gaactgaggt ttgagaagga tatgcctaac
    20401 atttacctgg tcgtttcgtc gttcaatcat ctggatgatg tgcaatccgt actcggtttc
    20461 caccacctgg ctaatttgac cttccttaag gttaaatgcg gcatcggcaa atgccttaac
    20521 aagttcttcg cgtgaacgga aacccaactc tccgcccttt atggccgatg ctctatcctc
    20581 agagtagagg gcggcaaggg ttgaaaatcg ttcaccatta agtattcgat tacgaatttc
    20641 aagcagtttt tctttagcct caagcttagc cttttcggca tcgggggggt aaattgcaat
    20701 ctgttgaagt tcgtactgca tgggtatgtc gggtaggctg tcggagggaa ttttcttgta
    20761 aaaagctttt acgtccgatg gggttatttt aaccttatct acaatcttac gctgcatctg
    20821 ctgggttagc tgttgctcgc gtatgatttc tcttaaatcg tccttaattt caaatatcgg
    20881 cttgttaaag tagttctcca gtgccttttc cgatccaatt tgattgataa agtactttaa
    20941 acgacggtca acttcgcttt caactgaacc ttcgtttacc tcaatactat caattttggc
    21001 ctgggttatt agaagtttct gaattaggag ttgctccagt aactggcaac ggtcgatatt
    21061 agcatttgta ctgccttgca ttttaatgcg caggagttcc tgctcaacat cggatagaag
    21121 tatgcgctca tttccaacaa ctgcaattac cttgtcgata gagatatttt cctgcgcgtg
    21181 cgaactaaaa ctaaacgaaa aaatcaataa aaaaagtgta ggtaaactaa atgctctcat
    21241 ggttgtacta tttgtagatt tcaaaattac gatcgtcttt tgctttaata aacaaattgc
    21301 gttccagctg gtcaatcagg ttttgctttc gcgcgttaat aattagctgt tttatctcgt
    21361 ttgttatgta agctagaggt gcagtttcgc cttgctcttt aacctcacgc agcgaaacca
    21421 ggtagatgaa actgccgtct tccatttcaa tgtattgtct tgaaaaggtc tcctttatgt
    21481 tatcaattgt gtaaggaagc tgacgggtaa ttaccgagaa tggtatccat tggtcattga
    21541 aaaaatcgta gaaaatggcc gcctgatagg ccaaattatc cagtagttgt atgtcctcct
    21601 gtttcttaga acggtatatt tccttgatct tgggggtttg aggggcttcc ttgcggagct
    21661 ttatgaatag tgctttaact attggctctg agagaacaaa gttatccttg ttggagtagt
    21721 agtactgctc tacctctgtt tcctttacca gtgtatcgag tttttggttt acgtaagcct
    21781 gttcgtactt gtgtattagt agtgatgccc tataatcttc cagctgacgt gccacgtttt
    21841 tctccttatc ggtaaggttg ttctcggcct gttttagcag gagttgagtt tgaatccact
    21901 tctcaatgta agccttagca attagtatac tatcctcctt acttacattt gtgaggaaaa
    21961 tattttgaag gtcgctttcg tataggtact tatcgtatac ccttgctaca ggccggctat
    22021 tatcggcgtt ttggcaagca gtaagaaagc cagttactac aatcagcgaa aaaagtagtc
    22081 tattcatttt tttatttgtt gagattttca agtagcttac tgtaagtgtc attgttaata
    22141 tctaccttgt atttttcctt aagcgtttga atccatgatt cctccagata attctgataa
    22201 tcagccataa cttctgctct gcagtcgttt agctccttag gctcaccggt agttatttta
    22261 ctgatatgta ctacaaggtg attgttgttc tgaacactac tgttaatacc tgagttccat
    22321 tgggtataac ctgaaagcaa tggattatcg gggaagccta cctgttggtt gtaggtaaca
    22381 ttttgtgcct tcttattcca tttcttaacg agctcatcag gatttattgc cggattcttt
    22441 tttaggctct ttactagtgc ctgtgctatc ttactatcag cgctttggta aacagtgtag
    22501 taaatttttt cgccccacag atacttatcg cggtggttct cgaaaaatgt gttgagtgcc
    22561 gtgctatcag atgcttttgt ccatacctcg cggttcgata tctcaaaaag cagcataccc
    22621 tcgaggtatt cgttggcaag ctgtttaaat tcaggatatt tttggggtaa aagcgatttt
    22681 tcaaagttaa ttatggtttg gtttacccaa tcggtataga tagaactgat gaacgactgt
    22741 aaggaattgc ccttgctttt ttgctggttt tggccgataa actgaagcaa gtccgatagt
    22801 gtataattca acccttttac aaccagtaaa gttggattgg ttttgatgtt tggagctttc
    22861 cagttgcctt taaaaatgga ggaatcgata agcgatgaga gatgcttggc cgattcggca
    22921 gggaactcct cgaccccgta ctctttttta agctttttaa tgaatgagga gcgtatgagc
    22981 atggatcggc tgtcgcgagc cattaggctt ttaatttcgg gcagcatctc ctcgaactcg
    23041 cccggtagct ttctatcaag tagtttaacc acgtgccatc caaatgaggt ttggaaaggc
    23101 ttggagatat cgccaatatc ctttagggca aaagcaacct cctcgaactc aggtattatt
    23161 tgcccggaac gaatccaggg tagttcgcca ccgtttgggg ctgtgctgtt gtcctgcgat
    23221 tcctttttgg ctaactcggc aaagttttcg ccgttaagta ggcggttatg aatgtcgact
    23281 attttttggc gggcgttttc catttcctga ggcgaggcat tggggttaac cctcaccatg
    23341 atgtgggcaa ccttaatttg ccccttggca gggcgacgac cgtgaacctt aatgatatgg
    23401 taaccaaagc gggtgcgaac cggcatcgaa acggaatcta ccggagtatt gtaagctgca
    23461 ctttcaaagg ggtaaaccat ttgaaaggct gagaaccaac caaggtaacc attgtttcgg
    23521 ctaaccgaag gatcgttgct agtggttaag gcaactttgc caaagggttc gccgctaaga
    23581 actttggagc gagcctcaat ggctttttgg tagtacttaa gagtatcgga acctgttact
    23641 gtttccggaa tgctaataag gatgtggctg gcgcttacct cttccagcat acgctggtag
    23701 gcctcacggg ttaacccatc gagcgtttcc tgatcaatga ggtacgattg cgcaagctga
    23761 tcaacataac cattatactc attcctgaac gaatataggg tatcaattcc tgcatcacgg
    23821 gctgcttgaa cctttagctt aaagttgatg aaaaggttta ggtagtcatc aagggtcgat
    23881 ttatcaattt ttgcaccagt agccttggtg tttttaatgt aaagcgatat aaactcatca
    23941 gctgaaatag gagtattgtt gatggttaaa agcgtttcag agccctgttt ctgggcataa
    24001 gcaatttgtc ctgctaacac tccggctgta atgattgcta ctcgaaatac ctttttcatc
    24061 ggttttgggt ttaagggtta ccttacaaaa cattagtttg gactaatagc tggtatcgcg
    24121 actatagttt ggagcctcgc gtgtgattgc cacatcgtgg gggtggcttt caataatacc
    24181 tgcatgagta atccgaacaa acttggcttt tttgagtgca tcgaggtttg gtgcgccaca
    24241 gtagcccata ccggcacgta ggccgccaat aagctggtag aaaacctcgg cgagggttcc
    24301 cttgtaggga acgcgagcca caatgccttc gggtacaagt tttttaatgt cgtcctcaac
    24361 atcctggaag taacggtcct tggaaccctg ctgcatggcg tcgagtgaac ccattcctct
    24421 gtacgacttg aacttacggc cattgtaaat gattgtttcg cctggtgatt cttccacacc
    24481 tgcaaaaagc gatccggcca taatggtatc ggcgcctgcc gctagggctt ttacaatatc
    24541 gcctgagtaa cgaattccac catcggcaat tacaggaacg cctgagcctt gaatggcact
    24601 tgcaacgtcc caaatagcgt ttagctgagg aacaccaacg cctgcaacaa ctcgggttgt
    24661 acatattgaa cccgggccaa tgcctacctt aaccgcatcg gctccagcat caacaagcat
    24721 tttagcggca tcggctgtgg ctatgttccc aacaatcact tcaagcttgg ggaattcctt
    24781 tttcaccatt tttagcatct caatcacacc cttgctgtgg ccgtgtgcag tatcaataac
    24841 aatggcatca acatc
//
LOCUS       DAOSAL010000032        24523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_1480_length_24523_cov_48.064492, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000032 DAOSAL010000000
VERSION     DAOSAL010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 24523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24523
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1480_length_24523_cov_48.064492"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(768..1268)
                     /locus_tag="PK049_06100"
     CDS             complement(768..1268)
                     /locus_tag="PK049_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61165.1"
                     /translation="MNYLKYFAFALSAAVMVLGSFSCDKNDENEIKKDTLYVKFINDP
                     ESQYTITTLQIRNRGPVDSQLQPTEPWSENILKNGQRIAPGQHIYFTLDLPSGHWAEY
                     RLGVDNGSGVEVMLYDQPNYDGFTNLPITHWATDNRTVTVTITYDNYSQTITVSGWSD
                     MAGIDE"
     gene            complement(1415..2179)
                     /locus_tag="PK049_06105"
     CDS             complement(1415..2179)
                     /locus_tag="PK049_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HOK61166.1"
                     /translation="MAKEKIKPAEQSTELSIEEKLRFLYKLQQVDSKIDKIKLLRGEL
                     PLEVQDLEDEIEGLTTRIDNYKKEIHDLETQINQKKIEIKDAEALIKKYQEQQKNVRN
                     NREYDSLTKEIEYQTLEIELCNKRIKEYTQQIKDKKQLVEQTEKTVEERTHDLNGKKQ
                     ELENITQETQKEEDELYRLSEEYAMNIEPRLLTAYKRIRGNARNGLAVVTVQRDACGG
                     CFNKIPPQRQLDIKMHKKVIVCEYCGRILVDDSLLE"
     gene            complement(2183..3277)
                     /locus_tag="PK049_06110"
     CDS             complement(2183..3277)
                     /locus_tag="PK049_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOK61167.1"
                     /translation="MTVREVTSILESFAPLAFQEDYDNSGLIVGDANMEVTGVLLTID
                     VTPQVVAEAVAGRANLIVAHHPVIFKGLKRITGKSYVEQVVVNAIKNNVAIYAAHTNI
                     DSVQGGVSFKMAEVLGLTSTQVLSPLTGQLVKLVTFVPTEYAEAVRQAMFNAGAGVIG
                     NYDFCSFNIQGQGTFRAGDGTNPFVGEKGKIHFEPEVRVETIVPRHILNAVVKAMVEA
                     HPYEEVAYDVYPLDLPYKKAGLGVVGDLPEPMNTIDFVKHVKQQFGAPCVRYTNPVKD
                     FISRVAICGGSAISLLNDAIAANADVFITADVKYHQFFDAENRIVIIDIGHFESEQFT
                     VDIFYDLLSKKISNFAVLKSKVRTNPINYI"
     gene            complement(3284..3622)
                     /locus_tag="PK049_06115"
     CDS             complement(3284..3622)
                     /locus_tag="PK049_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOK61168.1"
                     /translation="MPYKEKKIEKYYYSIGEVAEMLGVNTSLIRFWEKNFDVIKPHKN
                     KKGNRYFTKNDIENLKLIYHLVKEQGMTLEGARKKLSAQKEELTDNYKIVECLTEIKG
                     MLIEIRDLLD"
     gene            complement(3638..4609)
                     /locus_tag="PK049_06120"
     CDS             complement(3638..4609)
                     /locus_tag="PK049_06120"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOK61169.1"
                     /translation="MVKHKYKFHPEQLIFVRERKTIRDHLFRAAKYLLATFVIGFISY
                     LVFPIFIDTPEVRKLKRENQELLTNYELITRRIEQLNSVINNLQRRDEGIYRIIFEAE
                     PIPASIREAGIGGVNRYAELEKLSNSKLIIETTKKVDALTKKAYIQSRSFDEITDLAK
                     RKDEMLRSIPAIMPIRDKELTRVASSFGMRIHPFYKVLKMHNGMDFTAPTGTEIFATG
                     DGVVVDIVYAQRGYGNHVIIDHGFGYSTLYAHMSKVAVRKGQRVRRGMVIGYVGNTGT
                     SVAPHLHYEVHRNGQPVNPIHYYFNDLTPEQYEKLVDIASQPTQSFD"
     gene            complement(4616..5578)
                     /locus_tag="PK049_06125"
     CDS             complement(4616..5578)
                     /locus_tag="PK049_06125"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOK61170.1"
                     /translation="MAKFKYQFNPHTLKFDVIRIPLYKQFAKILIHLGFYAGVFLGLG
                     YAFSVIYDTPVEQSLKRANAEYVLKYELAQKQIFELSNALAVLEAKDNNIYRAIFEAD
                     TIPNTIRRGGYGGVDKYANLRSATNSLTLIKTLRALDELTWRAYVQSRSFDDVAEMAK
                     NKEKMILCVPAIQPINTKDLIRVSSLYGFRRDPINGAVTMHAGIDFVGSVGTPIYSTG
                     DGIVVEAGYSFNGYGNEVIVDHGFGYKTRYAHLSKVLTKVGQRVKRAEKIGLLGSSGK
                     STGPHLHYEVILRGKPVNPINYFNDMTEDDYQNMLKKYSSQFLD"
     gene            5730..8348
                     /gene="alaS"
                     /locus_tag="PK049_06130"
     CDS             5730..8348
                     /gene="alaS"
                     /locus_tag="PK049_06130"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOK61171.1"
                     /translation="MTSKELRQKFLDFFAGKSHQIVPSAPMVIKDDPTLMFTNAGMNQ
                     FKDIFLGNAPVKYPRVANSQKCLRVSGKHNDLEEVGHDTYHHTMFEMLGNWSFGDYFK
                     REAIDWAWEFLVDVLKIDKNRLYATIFEGSPDEGIERDLEAFECWKKHLPEGRILLGN
                     KKDNFWEMGDTGPCGPCTEIHIDLRDDDERKAIPGEQMVNKGHPQVIEIWNLVFIQFN
                     RKANGSLEPLPAKHVDTGMGFERLAMVVQGKKSNYDTDVFTPIIDRISQMCGIPYGTD
                     PKADVAMRVIADHLRAISFSIADGQLPSNVKAGYVIRRILRRAVRYGYTFLNFREPFI
                     DSLVAVLVEQMGDHYPELKAQQTLITNVINKEEATFLRTLETGIKLLEQLIEKAAKEN
                     RTEISGKDAFVLYDTYGFPLDLTELILREHNLSVNRSEFDAEMATQKERSRADAAVEA
                     DDWINVTNAEKVEFVGYDTLKTDTRIIRMRTVKTKGKEQYHVVLDRTPFYAESGGQVG
                     DSGLLESDSESIKVINTFKENNLIIHLVDRLPQNPNSPFFATVDYEKRIRTANNHSAT
                     HLLHNALRAILGTHVEQKGSLVHPDYLRFDFSHFQKMTDEEIRKVEQRVNKLIRQNIQ
                     LDEHRSVPMEQAQKMGAIALFGEKYGENVRVIKFGESVELCGGTHTKATGNIGYFKIV
                     SEGAIAAGVRRIEAITGEKAEEFVYSQIDTINQVKSTLGNPPNVIQAVSKLVDENNLL
                     KDQLNTLQAEKLKAVKAELKAKATERNGINLICERISIGNADAIKDLAFQLKNEVPNL
                     FLVLGTEADGKALLTIMINETLVEKHGLNAAAIVKEAAKEIQGGGGGQPFFATAGGKN
                     PDGLDKAIVKAKEAIK"
     gene            complement(8411..8986)
                     /gene="rsxA"
                     /locus_tag="PK049_06135"
     CDS             complement(8411..8986)
                     /gene="rsxA"
                     /locus_tag="PK049_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HOK61172.1"
                     /translation="MEYLIIIIAAIFVNNVVLSQFLGICPFLGVSNKIDTSIGMAGAV
                     TFVIVLATMVTFLIQHYVLNPLGVGFMQTIVFILVIAALVQMVEIMLKKLSPALYQAL
                     GIFLPLITTNCAVLGVAILVIKKNYNLLEGVVFGGATAIGFGLALVIMAGIREQLDLA
                     NVPKGMKGVPIALLTAGILALAFMGFAGIVK"
     gene            complement(8998..9576)
                     /locus_tag="PK049_06140"
     CDS             complement(8998..9576)
                     /locus_tag="PK049_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HOK61173.1"
                     /translation="MSKLYNLTKGIIKENPIFVIVLGMCPTLATTSSAMNGLGMGLAT
                     TFVLLGSNTVISLIARFIPDKVRIPSYIVVIATFVTIVDLVMQAYTQSLYEKLGIFIP
                     LIVVNCIVLGRAEAFANKNSVFDSILDGIGMGLGFTFALTLLGSVREILGAGSLFDHK
                     FISGDAILVFILSPGAFIALAYLIAIFNRLKK"
     gene            complement(9569..10150)
                     /locus_tag="PK049_06145"
     CDS             complement(9569..10150)
                     /locus_tag="PK049_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HOK61174.1"
                     /translation="MAKVESNFKNMTATLLVITLVASAALGLVYQSTKEPIALAQQAK
                     INNAIKAVLPEFDNQPGNEAYTVAVDGGELTFYPATKEGQPVGTAVKTFSNNGFAGRI
                     ELMVGFLPDGTINKIAVISHKETPGLGDKMDPKKSNFSLQFEGKNPETFKLKVKKDGG
                     DVDAITASTISSRAYCDAVIRAYNALKEGGKHE"
     gene            complement(10143..11147)
                     /locus_tag="PK049_06150"
     CDS             complement(10143..11147)
                     /locus_tag="PK049_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOK61175.1"
                     /translation="MDSKLVVSPSPHVHSGESTSRLMFNVVLALMPAFAVSLYYFGVG
                     ALIVTLLSVLSCVLFEYLIQKFILKVQPTITDGSAIVTGLLLAFNVPSNLPWYIVVIG
                     SLFAIGVAKMSFGGLGSNPFNPALAARVFMLISFPVQMTSWPLPIESRLKYIDAITGP
                     TPLAILKEGLKKGDTISQIADQIPSHLQLFMGVMGGSLGEISALALLIGFAWLLYKKV
                     ITWHIPISIFTTIFVFTGILWLINPEQNADPIFHLVTGGVMIGAIYMATDYVTSPMHP
                     KGMLIFGIGIGLLTVIIRVWGAYPEGVSFAILIMNAFVPLINRYVKPKRFGEEVKRNG
                     "
     gene            complement(11179..12510)
                     /gene="rsxC"
                     /locus_tag="PK049_06155"
     CDS             complement(11179..12510)
                     /gene="rsxC"
                     /locus_tag="PK049_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOK61176.1"
                     /translation="MLKTFAKGGVHPPENKFSADRPIEVLPLPAKVSIPVSQHIGAPA
                     TIVVAKGDKVKVGQLIAQSAGFVSANIHSSVSGTVESVDPVLDSSGYKRQAVTIVVEG
                     DEWEPTIDRSPELKRTIDADAQTIIKKIAEAGIVGLGGATFPSHVKLSVPTGKKADVL
                     IINGVECEPYLTSDHRLMLEKGHELMVGIQLLMRALGVDKAMIGIENNKPDAIKYLSD
                     ISKDYQGISVHALKVKYPQGGEKQLIKALINREVPSGGLPIDIGAVVHNVGTAFAVYE
                     AVQKNKPLFERVVTVTGKNLAKPSNFLVRIGTPVSDLIAAAGGLPDDTAKVVNGGPMM
                     GKAVTDISVPVTKGTSGIIVFPANESRRKADSVCIRCAKCVTACPMGLEPYLLIRMSQ
                     RMMFDKMEHERVLDCMECGSCSFECPANLPLLDYIRLGKSEVNKIIRARKN"
     gene            complement(12539..13405)
                     /locus_tag="PK049_06160"
     CDS             complement(12539..13405)
                     /locus_tag="PK049_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018668709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster domain-containing protein"
                     /protein_id="HOK61177.1"
                     /translation="MNSIIITAVLVLSLLGVALAIVLYLVAQKFKVIEDPRIDLVEAV
                     LPGANCGGCGYPGCRGFAEAFVKTDDIGSMFCPVGGNDTMAKAAEVLGQTAEAKDPMI
                     AVVRCSGSPEQRKRLVEYDGASSCKLATALYGGDTGCQYGCLGLGDCVAVCKFDAIYI
                     DPVTLLPVVDEEKCTACGACVKACPKFIIELRKKGPKGKRIFVSCINKDKGGPARKAC
                     QVACIGCGKCVKVCPHDAITLENNLAYIDYNKCKLCRKCVEECPTGAILEINFPPRKQ
                     KVEGQAENVENN"
     gene            complement(13424..13846)
                     /locus_tag="PK049_06165"
     CDS             complement(13424..13846)
                     /locus_tag="PK049_06165"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HOK61178.1"
                     /translation="MSLKPNVIEHLGRVEAITATDIRVTIISQSACASCQAKGACSAA
                     DVAEKQVVVSKPYHNYTVGETVKVTMQQSLGFKALFLGYILPLIVLVVSLFVFNALGF
                     SESVSGLLSLAVLVPYYLMLYLFRKSIEKQFHFDIEKL"
     gene            14553..14735
                     /locus_tag="PK049_06170"
     CDS             14553..14735
                     /locus_tag="PK049_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61179.1"
                     /translation="MGAPRVGLPRDTIRHACGVAHVVSLTRPIATFHSRPISGSGFYA
                     IACFFLSIPSQGKGLG"
     gene            14994..17306
                     /locus_tag="PK049_06175"
     CDS             14994..17306
                     /locus_tag="PK049_06175"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOK61180.1"
                     /translation="MKLLIAVFKRFFFLLGLPLLVLTGYAQGVVENNPLFDDSEVARI
                     DITINPTYLQSILQAGNEESNTEYPATFSFTSSKFTAVVENAGFRLRGNTSRYAQKKS
                     FKVSFNTFEPGNKLKGIEKLNLNGEHNDPSIVRSKLVWDLMGEIGLPAPRANHVRLYI
                     NNQYYGLYINVEHIDENFVKARFGNNDGNLYKCLYPADLTYRSSDPNAYKFVQNGYRV
                     YDLKTNTEQDNYSDLAQLIDIINRTSISDLPAKLEPIFDVNNFLKYLAVEALTGNWDG
                     YSYNKNNFYLYRNTATGRFEFIPYDTDNTFGIDWFNIDWATRNVTTWVSSQARPLTKN
                     ILAVDVYRKRYYFYLKQLINGAFSTNSIQSKAIALRSKIEAYATEDPFRPLDYGWSSS
                     DFYSSYFNALGGHVKYGLIPYVTKRIQYANSQFSIGDIPPIVSNVSWLAYGYKVPVTV
                     FADVDDEEKANIKLYFKADDSNWQSFEMQNTNRNHYISTLGPWSKPVKAISFYLECTD
                     KSGKTTREPLIGEYSVKFNQIAIPICINEVMTSNQSTYPDEYGNYSDWVELYNYGKNP
                     ISLQGMSISDSLGKPGKWALPSITINPGEFLLLWADGEPEKGNNHLPFRLSNQGEEIG
                     LFTSKTDGYKLIDGYQFGKIAKNESFGYYPNAVGLPQALLSPTPGKSNVYTSKRIDMV
                     LPEIIAYPNPFTESVTITLSYQPAYDYTISIVNLQGITVLEHYMKKDDNPKLTWRPQN
                     LSKGTYLIVFTIHGKQNQLIKPQKVIFEKF"
     gene            17311..18015
                     /locus_tag="PK049_06180"
     CDS             17311..18015
                     /locus_tag="PK049_06180"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HOK61181.1"
                     /translation="MIRNKLALIFASTLLIVTNASSQVARTLWSGAELQLPFSKAFRV
                     TFSPELRLTDEKRIDDFILEAEAQFRIHKLLQPTFSYRYYRFYDDPGEYFNGQRLGAG
                     INSSVELGRFKLTNRIYYFHRLINRYGYGYSIETRRELREGIKITYNIRRTKLEPFVQ
                     AEIYYDISPDRNHEFSRVRIRTGLEYPFSKRSSIDLFFQMQDKLNTSNPLRTYTIGIF
                     YNYKLPTPKPKSVDIE"
     gene            18074..20035
                     /locus_tag="PK049_06185"
     CDS             18074..20035
                     /locus_tag="PK049_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOK61182.1"
                     /translation="MISVNNLTLSFGGFDLFKGISFQLNDNEHVGLVGKNGAGKTTLL
                     RLIAGELQPTKGSISLPSDIRLGYLPQQMKLASGKTVIEETRKAFAETIQIKNDINHI
                     SEQISHRTDYESKEYLELINKLTELNDRYAILEGDSADARAEQTLLGLGFKRADFSRQ
                     TTEFSGGWRMRIELAKILLQNPQVLLLDEPTNHLDIESIQWLEDYLKDFQGALLLISH
                     DRAFLDNVTQRTIEISLGNVYDYRVPYSQYVELRRERREQQMAAYRNQQKLIEDTQEF
                     IDRFRYKATKAVQVQSRIKMLERMDIIEVDEEDSATLNIRFPEAPRSGDVVIEAKEVS
                     KSFGNKHVFSDVHFTIERGQKVAFVGRNGEGKTTMSRIIVGELPYDGHLKIGHNVTIG
                     YFAQNQDELLDGSLTVLETIDHVAVGDIRTRMRDILGAFLFRGEDVDKRVSVLSGGER
                     NRLAMAKLMLQPYNLLVLDEPTNHLDMRSKDVLKNALQAFNGTLILVSHDRDFLNGLV
                     DKIYEFTDGKVKEHLGGIYDFLQRKKLQNLKELERKQVEQKAKNSTSANSEQKIQWEE
                     RKELDRQIRKLESQVEKLEKEIGMLESTIAETDTKLSNPTAHGIDLSDKDFFKKYESH
                     KTELMGKMEQWENLLAQVEELKTKRNNTN"
     gene            20038..20778
                     /gene="rlmB"
                     /locus_tag="PK049_06190"
     CDS             20038..20778
                     /gene="rlmB"
                     /locus_tag="PK049_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOK61183.1"
                     /translation="MENSENLIFGIRAVLEALNAGAKISKVIVRQGLTGNLASELMGL
                     LKHNNIPFQYLPNEAFTRYKDKNHQGVIAFTSPIEVYRVEDILPQLQEKTNSPFLLIL
                     DSITDVRNFGAIVRTAECAGVDAIIVPAKGAANIGGDAIKTSAGALYNIPICRVGSLK
                     MLSYQLRDYGYRIVSASERGEKLLYEANLTGPIAIVMGAEDTGIHPEVLRLSHEIVRI
                     PLKGKIESLNVSVATGIVLFEAVRQRTL"
     gene            complement(20773..21696)
                     /locus_tag="PK049_06195"
     CDS             complement(20773..21696)
                     /locus_tag="PK049_06195"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOK61184.1"
                     /translation="MVKSKNVALVLGSGGARGLAHIGVIEELTRRGYSITSISGSSMG
                     ALVGGLLAANRLDDYKNWILKLDKIDILTLIDFTFSSKGIVKGQKVFEKMQELNMVPD
                     VNIEDLPLPYTAVAVDILSNQLIVFNSGKLQTAIRASISIPSVFTPLPYNGTLLVDGG
                     VLSPLPLEYVRRTKNDLLVAVDLNAIIPYDLAVPTNKQSKNSWVKDNPKIEQLVAKWD
                     KFFGHNAAAKESEVKAKQSPKSIGYFDLAMRSVQLMQSQLTQYAIKVTPPDVLVPISK
                     NSATVFEFYKAAELIEYGRLQCAKVLDEKGL"
     gene            complement(21776..22249)
                     /locus_tag="PK049_06200"
     CDS             complement(21776..22249)
                     /locus_tag="PK049_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012457472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOK61185.1"
                     /translation="MSEHEKFMDEAIRLAVDGVMAGDGGPFGAIVVKDGKIVGRGNNH
                     VTSTNDPTAHAEVMAIRDACKNLNTFQLNGCVLYTSCEPCPMCFGAIYWARLDKVYYS
                     ANRHMASYHGFDDSFIYDEVNLSFDRRRIPFIHVSPEKGVVPFREWDNKENKVKY"
     gene            complement(22362..23639)
                     /locus_tag="PK049_06205"
     CDS             complement(22362..23639)
                     /locus_tag="PK049_06205"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOK61186.1"
                     /translation="MEGMEEQVIRKYLFLEDLESWLLSQGINETFAQFFKVAISLAAI
                     FILALIADFIAKRIFLTTMTSVARRTKTDWDDVLVEKRFFHRLAHFAPAIVVYLTLGI
                     ALYDYSPKVTLFLQALTKIYMIFIAISVFNAFLDTVNEIYQELPYAKNRPIKGYLQVL
                     KIIIYVFAGILIIGIIINKNPASILVGLGASTAVLMLVFKDVITGLVASIQLSANEML
                     KLGDWIEMPGRNLDGTVVDISLTTVKVQNFDNTISTVPPFALITESFKNWRGMEESAG
                     RRVMKSIIIDSKTVKFCDKELIQKLSNINLIASKLKDIDVDKLHETDPIQLMNAGAQT
                     NLNLFRQYVQEYLNHHPEVNKHLTLVVRLKQPVEYGIPMEIYCFLKEKRFVQYEIIQS
                     EILDHIMAALPVFDLKVFQRMSSDDIRAIAKGN"
     gene            complement(23685..24050)
                     /locus_tag="PK049_06210"
     CDS             complement(23685..24050)
                     /locus_tag="PK049_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HOK61187.1"
                     /translation="MEIQGKLIKKLDQQTGHGKNGAWVKQEFIIETQEQYPRKICISL
                     WGDKVRELDPIQEGSQIKVAINIESREFNGRWYTDVRAWRIEPMSKGATPEQAPEPPP
                     FNDNLYPPMEGGSDDGLPF"
     gene            complement(24116..>24523)
                     /locus_tag="PK049_06215"
     CDS             complement(24116..>24523)
                     /locus_tag="PK049_06215"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005640538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOK61188.1"
                     /translation="EASNKFLDENKTKEGVVVDSSGLQYKIIEEGTGLMPTAEDVVKV
                     HYKGTLIDGTQFDSSYDRGEPAQFKLNQVIPGWTIGLQKMKVGSKFILYIPSELGYGE
                     RVRPGSPIGPNQALIFEVELLDIVKENAQDSKK"
BASE COUNT         7134 a         5566 c         5209 g         6614 t
ORIGIN      
        1 tgcgatgcaa cgcatctgaa ttgctctttg gaaaagttgt tgcgtgcatc gcaaaataat
       61 ttgaacacct caatgccctg ggttaaaacc cagggctaat gtgatttgcc ctttcagggc
      121 gatttatgtg tgcgtctaaa gacgtgtgct aactagtgag ttcttaaatc ttaaatcgag
      181 atcactcgac tagctcggaa tgacaatgga gttgtaaatg tttcgcccct acggggctgg
      241 gaacggcgtt cgttgttcgt gtatcgttgt ttgtgaatgc tacgttatac tctctgtgca
      301 acacgtgtta cactgtgtaa cactgtgata cttgctgttg ctcggttcat gagtgggttg
      361 tcatgctaag ctaagtcgaa gcatctcttt ccccttttac ccttatatga gatccctcga
      421 ctacgctcgg gatgacaaca tcatggctcc ctcactcact ttatcatgtt gagcgcaagc
      481 gaaacatctc atggggtgga ttagagatcc tttgctcccc gctagggcgg gacaggccac
      541 tcaggatgac aagcggagtt gacagttgat ggttgatagt tgatagagaa aaaaacattt
      601 cacgaaacac tacaaaccaa acactaaaca ccaaacacca aacactaaaa acccctgtac
      661 actgcacact attcaaaaga tccttcgctg cgctcaggat gacaaaagca atgtcagttc
      721 tgctatctaa acgacattga atccggaatt agtcctgtag taagctgtta ttcgtcaatt
      781 ccagccatat cggaccatcc gctaacggta atggtttggc tgtagttgtc gtatgtgatg
      841 gtaacagtaa ccgttctatt atcggttgcc cagtgggtaa tgggcaaatt ggtaaagcca
      901 tcgtaattgg gttgatcgta aagcataacc tctacaccac taccattgtc aacccctaaa
      961 cgatattcag cccagtggcc gcttggtaga tcaagggtaa aatagatatg ctggccagga
     1021 gcaatccgtt gtccattctt aagaatgttt tcactccagg gttcggttgg ctgaagctgt
     1081 gaatcaactg gtccacggtt tcggatttgc agggttgtaa tggtgtactg cgattccgga
     1141 tcgttgatga actttacata cagggtatcc tttttgattt cgttttcatc atttttatcg
     1201 cacgagaatg agccgagaac cattaccgct gctgacagcg caaacgcaaa atactttaga
     1261 tagttcatat tttaaaggat ttgaaatttt ttgcaatgta aacaaaaatc aatactatat
     1321 gttgaaatgc cgcattaatc cgataaggta aacttaatga ataaataaaa cgcccattgc
     1381 agggcgttaa tggtattgat gataattaat tagcttattc gagcagtgaa tcatcaacca
     1441 gaatacggcc gcagtactcg caaacaatga cttttttgtg cattttgata tcgagctggc
     1501 gctggggagg aatcttattg aagcaaccgc cgcaggcgtc gcgctgaaca gtaaccacag
     1561 caaggccatt acgggcattg cccctgatac gtttgtaggc agtgagcaat cggggttcaa
     1621 tgttcatggc atactcttcg ctcaggcggt aaagttcatc ctcctctttc tgggtttcct
     1681 gggtgatatt ctcgagttcc tgtttcttac cgttcaaatc gtgtgtacgc tcttcaacgg
     1741 ttttctcggt ttgctcaacc agttgctttt tatcctttat ctgttgggtg tactccttga
     1801 tgcgcttgtt gcaaagctca atttcaaggg tttgatattc aatctccttg gtaagtgagt
     1861 cgtactccct gttgttgcga acgtttttct gctgctcctg gtacttcttg ataagagcct
     1921 cagcatcctt gatttcaatt tttttctggt tgatttgggt ttccaaatcg tggatctctt
     1981 tcttgtagtt atcgatacgg gtagtaagac cctcaatttc atcttccaga tcctgaacct
     2041 caaggggaag ctccccgcgt agcaatttaa tcttatcaat tttggaatct acctgctgaa
     2101 gcttgtaaag gaaacggagt ttctcctcaa tggacagttc agtgctctgc tcagccggtt
     2161 tgatcttctc tttagccata atctatatgt agttaatagg attagttcgt actttggatt
     2221 ttaagactgc aaaattagat attttttttg agagtaaatc ataaaaaata tcaacggtaa
     2281 attgttcgct ttcaaaatgt ccgatatcaa tgattacaat ccgattttcg gcatcaaaaa
     2341 actgatggta tttgacatcg gcggttataa aaacatcggc attggcagca atagcatcat
     2401 tcaaaagcga aatggcgcta cccccgcaaa tggcaaccct ggaaataaaa tccttaacag
     2461 gatttgtgta cctaacgcaa ggagctccga attgttgctt tacatgctta acaaaatcga
     2521 tagtattcat tggctcaggt aagtcgccta ccactcccaa cccggccttt ttatagggta
     2581 aatcgagggg gtaaacatcg taggcaacct cttcgtaggg atgagcctca accatagctt
     2641 taaccacagc gttaagtata tgacggggca caatggtctc aaccctaact tcgggctcaa
     2701 agtggatttt gcccttttcg ccaacgaacg gatttgtgcc atcgccagcc ctgaaggttc
     2761 cctggccctg aatattgaag gagcaaaaat cgtagtttcc aataacccct gcgccagcat
     2821 tgaacatagc ctgccgaaca gcctcagcgt actcagtagg aacaaaggta acaagcttta
     2881 cgagttgacc ggtaagcggg cttaacacct gtgtgctggt taagcctaaa acctcagcca
     2941 ttttaaagct aacaccaccc tgcacactgt cgatatttgt atgggcggcg taaatggcaa
     3001 cattgttttt tatggcatta accacaacct gttcaacgta acttttacca gttatgcgct
     3061 taagcccctt aaaaattaca gggtgatgag ccactatcag gtttgccctt cctgcaacag
     3121 cctcagcaac tacttggggg gtaacatcaa ttgtaagcaa aacaccagtc acctccatgt
     3181 tagcatcgcc aacaataagg ccggagttat cgtaatcttc ctgaaaagca agcggagcaa
     3241 acgattccag gattgaggtt acttctctaa cagtcataaa cgcttaatcg agcaaatcgc
     3301 gaatttcaat gagcatgccc ttgatctcgg tgaggcactc cacaatcttg taattatcgg
     3361 taagttcctc cttttgagcg gaaagctttt tacgggcacc ttcaagggtc attccctgtt
     3421 ctttaaccag atggtaaatc agtttcaggt tttcgatatc gttcttggta aagtagcgat
     3481 tgcctttttt gttcttgtgg ggctttatta catcaaaatt tttttcccag aagcggatta
     3541 acgaggtgtt tacgcccaac atctcggcca cttcgccaat ggagtagtag tacttttcaa
     3601 tttttttctc tttgtatggc attttagtaa ggattggcta atcgaaactt tgtgtgggtt
     3661 gcgaggctat atcaaccagt ttctcgtatt gctcgggtgt taagtcgttg aagtaatagt
     3721 gaatggggtt aacgggctgg ccattccggt gcacctcgta gtgtaaatgg ggagccaccg
     3781 atgttccagt gttgccaaca tatccaataa ccataccgcg cctaacacgc tgcccctttc
     3841 gtactgcaac cttgctcatg tgagcgtaaa gggttgaata accaaagccg tggtcaataa
     3901 taacatgatt gccataacca cgctgagcat aaacaatatc aaccactacc ccatcgccag
     3961 tagcaaatat ttcggttcct gtaggtgcgg taaagtccat accgttatgc attttaagca
     4021 ccttatagaa aggatgaatg cgcatgccaa acgatgaggc cacacgggtt agctccttat
     4081 cgcgaatagg cataatggca gggatgctgc gcagcatctc gtccttgcgt tttgccaaat
     4141 cggttatctc atcgaatgag cggctttgga tgtatgcttt tttggttagg gcatcaacct
     4201 tcttggtggt ttcaataatc agcttgctat tggatagctt ctccagctca gcataacggt
     4261 ttacaccacc tataccggcc tcgcgaattg aggcaggaat gggttcggcc tcaaaaatga
     4321 tacggtagat accctcatcg cgacgctgta aattgttgat tacggagttt agctgttcaa
     4381 tacgacgggt tataagctcg tagttagtaa gcagctcctg attttcgcgc tttagcttac
     4441 gaacctcagg ggtatcgata aatattggga aaactaagta ggaaataaaa ccaattacaa
     4501 aggtggccag taggtatttt gcagcacgga aaaggtgatc tcttatggtt ttcctttccc
     4561 tgacaaagat cagctgttcc ggatgaaatt tatacttatg ctttaccata tagccttaat
     4621 ccaaaaattg acttgaatac ttcttgagca tattttggta gtcgtcctcg gtcatgtcgt
     4681 taaagtagtt gattggattc acgggtttac cacggagaat aacctcgtaa tggaggtgtg
     4741 ggccagtgct tttacctgaa ctacccagta atcctatctt ctctgcccgt ttaacccgtt
     4801 gtccaacctt tgtcagcacc ttactcaggt gggcatagcg tgttttatag ccaaagccat
     4861 ggtcaactat aacctcgtta ccatagccgt tgaaggagta accggcttca actacaatac
     4921 cgtcgccggt ggaatagata ggagtaccaa ccgagcccac aaaatcaatt cccgcatgca
     4981 tggtaacagc cccgtttatg ggatcgcgac ggaaaccgta caagcttgaa accctaatca
     5041 gatcctttgt gttgatgggc tgaatggctg gaacgcataa aatcatcttt tccttattct
     5101 tggccatttc agcaacatca tcaaaagagc gcgattgaac gtaggctcgc caggtaagct
     5161 catcgagcgc ccttagagtc tttataaggg ttaatgagtt ggtagctgaa cgtaggttag
     5221 catacttatc cacaccgcca taaccacccc tacgaatggt gttaggaatg gtatcggcct
     5281 caaaaatagc ccggtaaata ttattgtcct tggcttcaag taccgccaaa gcattgctca
     5341 gctcaaatat ttgcttttga gccaactcat atttaagtac gtactcagca ttagcccttt
     5401 taagcgactg ctcaaccgga gtatcgtata taacagaaaa agcatagcct aaccccaaaa
     5461 aaacacctgc ataaaatccc aaatgaatta gtatctttgc aaactgtttg taaagaggta
     5521 ttcgaataac atcgaatttg agtgtatggg ggttaaactg gtatttaaat tttgccatgt
     5581 cctttaacac aaactgttac aatgtaacac acttttgaca aactgcaaca aaactatggt
     5641 aaaaatattt tttacacaag taaaaagaac aattttttta ctttaaatag ttaaatagag
     5701 aatattaaca ttttcaatac taacttttta tgacttcaaa ggaattgcgt cagaaatttt
     5761 tagacttttt tgctggcaag tcgcaccaaa ttgtgccttc ggcaccaatg gtaattaagg
     5821 atgacccaac gctgatgttc accaatgcag gcatgaacca gtttaaggat attttccttg
     5881 gcaacgcacc ggttaagtat ccccgagtag ccaactcgca gaaatgcctc agggtatcgg
     5941 gcaagcataa cgatttggaa gaggttggtc atgataccta ccaccacacc atgtttgaga
     6001 tgcttgggaa ctggtcgttt ggcgattact ttaaacgcga agccattgat tgggcctggg
     6061 agtttctggt tgatgtgctt aaaatagata agaacaggct ttacgcaaca atatttgagg
     6121 gtagccctga cgaaggaatt gagcgcgacc ttgaggcttt tgagtgctgg aagaaacacc
     6181 tgcccgaagg cagaatcctt cttggaaata aaaaggataa tttctgggaa atgggcgata
     6241 caggaccttg cggaccctgt accgagattc atattgattt acgcgatgat gatgagcgta
     6301 aggctatacc cggtgagcaa atggttaata aggggcatcc ccaggtaatt gaaatctgga
     6361 accttgtatt catccaattc aaccgtaaag ccaatggttc attagagcca cttccggcca
     6421 agcatgttga tacgggtatg ggctttgaac gtttggccat ggtggttcag ggcaaaaaga
     6481 gcaactacga tacagatgta ttcacgccaa ttattgaccg tatatcgcaa atgtgcggaa
     6541 taccctatgg gactgatcct aaggcagatg tggccatgcg cgttattgct gaccacctca
     6601 gggcaatcag tttttcaatt gccgatgggc agctaccatc caacgttaag gctgggtatg
     6661 taatacgccg cattctccgt agggcggttc gatacggtta cacattcctg aacttccgcg
     6721 aaccgtttat cgatagcctt gtggccgttc tggttgaaca aatgggcgac cactaccctg
     6781 aacttaaggc tcagcaaacc ctcatcacca atgtaatcaa caaggaggag gcaacattcc
     6841 tgcgcactct tgaaacgggt atcaagctgc ttgagcagct tattgaaaag gctgcaaagg
     6901 aaaacagaac cgagatatcg ggtaaggatg cctttgtgct ctacgatacc tacggttttc
     6961 cgctcgacct tactgaactc attttgcgtg agcataacct gagcgttaac cgctcagagt
     7021 tcgatgccga gatggctacc caaaaggagc gctccagagc tgacgctgca gttgaagctg
     7081 acgattggat taacgtgacc aatgcggaaa aggtagagtt tgttgggtat gacactctta
     7141 aaaccgacac ccgcattatc cgtatgcgaa cggttaaaac caaggggaaa gagcagtacc
     7201 acgttgtgct cgaccgcact cctttttacg ctgaatcggg cggtcaagta ggcgattcgg
     7261 gattacttga gagcgactcg gaatccatta aggtgattaa cacctttaag gagaacaacc
     7321 ttatcataca tcttgtagat agattgcctc aaaaccccaa ctcgccgttc tttgccacag
     7381 ttgattacga aaaacgaatt cgaactgcta ataaccactc agccacacac ctgctacaca
     7441 atgcgctacg cgcaattctg ggcacccatg tggagcaaaa aggttcattg gttcaccccg
     7501 attacttgcg tttcgacttc tcacacttcc aaaaaatgac cgatgaggag atccgtaagg
     7561 tagaacaacg ggtaaacaag cttatccgac aaaacataca gctcgatgaa caccgaagtg
     7621 ttcccatgga gcaggctcaa aaaatgggag ccattgccct atttggcgaa aagtatggcg
     7681 aaaatgtaag ggttattaag tttggcgagt cggttgaact ttgcggtgga acccacacca
     7741 aggcaaccgg taacattggt tacttcaaaa tagtttcgga gggcgccatt gcagccgggg
     7801 tacgccgtat tgaagcaatt accggcgaaa aagccgaaga gtttgtttac tcacagattg
     7861 atacaatcaa ccaggtaaag tcaactcttg gcaatccacc aaacgttatt caggctgtat
     7921 ccaaactggt tgatgaaaac aacctgctaa aagatcagct aaatacccta caagccgaaa
     7981 aactaaaagc ggttaaagct gaacttaaag caaaggctac cgagcgtaat ggtatcaacc
     8041 tgatttgcga gcgcataagc ataggtaatg ccgatgccat aaaggattta gcattccagc
     8101 tgaaaaatga ggtgccaaac cttttcctgg tattgggcac tgaagccgat ggcaaagcgc
     8161 tacttaccat tatgataaac gaaacgcttg ttgagaagca tggcctaaat gcagctgcta
     8221 ttgttaagga agccgctaag gaaatccaag ggggcggtgg cggacaacca ttctttgcca
     8281 cagctggagg taaaaacccc gatggattgg acaaagcgat agtaaaagct aaggaagcaa
     8341 taaagtagaa atcataaagg ctgccccaaa taaggcagcc tttaatttat tgtcaaaaaa
     8401 ctaaacgatt ctacttaaca atgcctgcaa agcccataaa ggccagagca agaatacctg
     8461 cggtgagtag agcaatggga acccccttca tccctttagg aacgtttgct aaatcgagct
     8521 gttcacggat tccagccatt attacaagtg ctagtccaaa accaattgct gttgcacctc
     8581 caaaaacaac cccttcaagc aggttatagt tctttttgat aaccagaatg gctacaccaa
     8641 gaacagcaca gtttgtagtg ataaggggaa gaaaaatacc tagggcctgg tataatgcag
     8701 ggctcaactt cttcagcata atctccacca tttgcaccag agcagcaatt accagaatga
     8761 acacaatggt ttgcataaaa ccaactccca aaggattcag tacgtaatgc tgaatgagga
     8821 aagtaaccat tgttgccagt acaattacaa aggtaacggc acccgccata ccaatagatg
     8881 tgtctatctt atttgataca cccaagaaag gacaaatacc aaggaattgc gatagaacaa
     8941 cgttgtttac aaatatggcg gcaattataa ttattagata ttccatactg tttcctccta
     9001 tttcttgaga cggttaaaaa tggcaatcag gtaggcaagg gcaataaatg cacccggcga
     9061 aaggataaat acaagaattg catcgcccga tataaactta tggtcaaaca gtgagcctgc
     9121 acctaggatt tcccttacag agcctaaaag agttaaggca aatgtaaagc ccagccccat
     9181 accaatacca tcgagtattg agtcaaatac gctgtttttg ttggcaaaag cctcggcacg
     9241 accaagaact atgcagttta caacaataag agggatgaaa ataccaagtt tctcgtaaag
     9301 cgactgggtg taggcctgca taaccaaatc gacaatggtt acaaatgtag caattactac
     9361 tatgtacgaa ggaatacgca ctttatcggg aataaagcgt gcaataagcg agattaccgt
     9421 attggaacct agcaagacaa aggttgttgc aagtcccatc cccaatccat tcatggctga
     9481 ggacgtggta gccagggtag gacacatccc aagaacaatt acaaatatgg gattttcctt
     9541 tattatccct ttagtcaggt tatatagctt actcatgctt acctccttcc tttaaagcgt
     9601 tatatgcacg gattactgca tcgcagtagg cgcggctcga aatggttgaa gcagtaatag
     9661 catcaacatc tcccccatcc tttttaacct taagcttaaa tgtttcgggg tttttcccct
     9721 caaactgaag gctaaaattt gattttttgg ggtccatctt atcgcctaaa ccgggagttt
     9781 ccttgtgtga gataacagca atcttattaa tggttccatc gggaaggaaa ccaaccatga
     9841 gttcaattcg acctgcaaaa ccattgttgc taaaggtttt tactgccgtt cctacaggtt
     9901 gcccttcctt tgtagccgga taaaaggtaa gttcgccacc atcaacagca acagtgtaag
     9961 cctcgtttcc gggttgatta tcaaactcgg ggagtacagc tttaatggca ttatttattt
    10021 ttgcctgctg cgcaagggca attggctcct tggttgactg ataaacaagg ccaagcgccg
    10081 cagaggctac cagggtaata accagcaggg tagccgtcat attcttgaaa ttcgattcca
    10141 ccttagccat tgcgctttac ctcctctcca aaacgtttag gtttaacata tcggttaatt
    10201 aatggcacaa aagcgttcat gattagaatg gcaaacgaaa cgccctctgg gtatgctccc
    10261 cataccctaa taatcactgt taacaaccct ataccaatac caaaaatcag cattcctttt
    10321 gggtgcatgg gtgaggtaac ataatcggtt gccatgtata tggcaccaat cattacacct
    10381 cctgtaacca agtggaaaat tgggtcggca ttctgttcag ggtttattag ccataggata
    10441 cccgtgaaaa caaaaatggt tgtgaaaata gatattggaa tatgccaggt tatcaccttt
    10501 ttatagagta accacgcaaa acctatgagt agagccaagg ctgatatttc gccaagcgaa
    10561 ccacccataa cccccatgaa aagctgaagg tgcgagggaa tttggtcagc aatttgcgaa
    10621 atggtatcac ccttttttaa cccttccttc agtatggcca ggggtgttgg gcctgtaata
    10681 gcatcgatat actttaaacg cgattcaatt ggcagtggcc aactggtcat ctgaaccggg
    10741 aaggatatca gcatgaaaac gcgagccgct aaagcggggt tgaacggatt gcttcccaac
    10801 ccaccaaagc tcattttggc aactccaatg gcaaataagc taccaattac aacaatgtac
    10861 cagggcaagt tgcttggaac gttaaaggca agcagcaaac ctgtaacaat ggctgaacca
    10921 tcggtaatgg taggctgaac cttaaggatg aacttttgaa taagatattc aaacagaaca
    10981 catgagagta ccgagagtaa tgtaactatt agggcaccta cgccaaagta gtaaagtgaa
    11041 accgcaaagg cgggcataag cgccaatacc acgttgaaca taagtcggct ggtcgattcg
    11101 ccgctatgca cgtggggtga tggtgaaact acaagtttac tatccattat ctttttaagt
    11161 ttggctggtt atgtcaaatt agttctttcg agccctgatg attttattca cttcgctctt
    11221 acctagcctg atgtaatcga gcaaaggtag gtttgctggg cactcaaagc tgcacgatcc
    11281 gcactccata caatcgagca cacgctcatg ctccattttg tcgaacatca tacgctggct
    11341 catacgaatg agtaagtatg gttcaagccc catgggacag gctgtaacac acttggcgca
    11401 gcgtatacat acgctatcag ccttacgtct cgattcgttt gcagggaaaa cgattatacc
    11461 cgatgtaccc ttggtaaccg gaacagaaat atcggttacc gctttaccca tcatggggcc
    11521 gccgttaacc accttagcag tatcgtcggg taaaccaccg gcagcagcta tcaggtcaga
    11581 aaccggtgtc ccaattcgaa ctaaaaagtt tgatggtttg gctaagttct tcccggttac
    11641 ggtaaccacg cgctcaaaaa ggggcttatt cttttgaact gcctcgtaaa ccgcaaaagc
    11701 tgtacccaca ttgtgtacaa ctgcacctat atctataggt aagccacccg atggaacctc
    11761 gcggttgatg agcgctttga taagctgctt ttcaccccct tgagggtatt ttacctttag
    11821 cgcatgaacg ctaatgcctt ggtaatcctt tgaaatatcg gaaagatatt taatggcatc
    11881 gggcttgtta ttctcaatgc caatcattgc cttgtcgaca cctaaggccc tcatgagtaa
    11941 ctgaataccc accatcagct catgcccctt ttcgagcatc aagcggtgat ccgatgtgag
    12001 gtatggttca cactcaacac cgttaattat gagcacatcc gcttttttgc cggttggaac
    12061 cgaaagttta acatgcgatg ggaatgttgc tccgccaaga cctacaatac cagcctcggc
    12121 tattttcttg atgatggttt gcgcatcggc atcgattgtg cgctttagct caggcgatct
    12181 atcaattgtg ggttcccact catcgccctc aactacaatt gttacagcct gacgcttgta
    12241 gccagaactg tcgagaactg gatcaaccga ttcaactgta cccgacaccg atgaatgaat
    12301 attggccgag acaaatcctg cgctttgagc aataagctga ccaaccttaa ctttatcgcc
    12361 cttagcaacc actatagttg caggcgctcc aatgtgctgc gatacaggaa tgcttacctt
    12421 tgcaggtagc ggcaacacct caatgggcct atcggccgaa aatttattct caggcggatg
    12481 aacgcctcct ttagcaaatg tcttcaacat tactatatgg ttatgttagg tgttacaatt
    12541 aattattttc tacattctca gcttgccctt cgaccttttg cttacgtggt gggaagttta
    12601 tttcaaggat tgcaccagta ggacactcct ccacgcactt acggcaaagc ttacacttgt
    12661 tgtaatcgat ataggcaagg ttattttcaa gggtgatggc atcgtggggg caaaccttaa
    12721 cacacttacc acagccaatg catgccacct ggcaagcctt acgggctggg ccacccttat
    12781 ctttgttaat acacgataca aagatgcgct tacccttggg cccctttttg cgaagctcaa
    12841 tgatgaactt tggacacgcc ttaacgcaag caccgcaagc ggtacacttc tcctcatcaa
    12901 ccacgggtaa aagggttacc ggatcgatat aaatggcatc gaacttacaa acggctacgc
    12961 aatcgcctaa gccaaggcaa ccatactggc aacctgtatc gccaccatat agggctgtgg
    13021 ccagcttaca cgatgatgcg ccatcgtatt ccacaaggcg tttccgttgc tctgggctac
    13081 ctgagcatcg tacaacggca atcatgggat cctttgcctc ggcggtctgg ccaagaacct
    13141 cggcagcctt agccatagta tcgttaccac ccacagggca aaacatcgat ccaatatcgt
    13201 cggttttaac gaatgcctcg gcaaagcccc ggcaaccggg atatccacac ccgccacagt
    13261 tagccccggg cagtacggcc tcaaccaaat cgatacgcgg atcctcaatt accttaaatt
    13321 tctgagccac aaggtataac actatggcaa gcgccacacc taaaaggcta agaactaaaa
    13381 ctgctgtaat aatgatagaa ttcatagatt aggttggttt tacttataat ttctcaatat
    13441 caaaatgaaa ctgtttctca atacttttcc taaataagta aagcataagg tagtagggta
    13501 caagaacagc taacgagagc aaacccgata ctgactcgct aaaccccaag gcgttgaaca
    13561 caaaaaggga aacaacaaga acgattaacg gaaggatgta tcccaaaaat aaagccttga
    13621 aaccaagcga ttgctgcatg gtaaccttaa cggtttcgcc tacggtgtaa ttatggtatg
    13681 gctttgatac tactacctgt ttctcggcaa catcggctgc tgaacaggcc cctttagctt
    13741 gacagctggc gcaagcggac tggctgatta ttgttacccg aatatcggtt gcagttattg
    13801 cctcaacacg tcctaaatgc tcaataacat tgggttttaa agacatctac ggaaaacgtt
    13861 tgaaatcaac cacaaaaata attttcttta gtatacaatt cagccgtaaa tgcaatttgg
    13921 aatcattcta aacatttgca aaataataat caatttcgat tagatagcca aacagactct
    13981 ccttttgtca tcctgagcgt agcgaaggat cttttgatta gtgtgcagtg tacaggggtt
    14041 tttagtgttt ggtgtttggt gtttagtgtt tggtttgtag tgtttcgtga aatgtttttt
    14101 tctctatcaa ctatcaacca tcaactgtca actccgcttg tcatcctgag cggcctgtcc
    14161 cgccctagcg gggagcgaag gagctctgac ctgccaaatg agatgtttcg ctaccgctca
    14221 acatgacaat agttttggga actgatcaaa aacaagtaac acagagttac acaatgtaat
    14281 acagtgtttc acagagtaaa tgacatgaca ttcacggaca acgaacaacg attcacgaac
    14341 aacgaataac gttctcagcc ccgtaggggc ggaacatttg taactaaatg aaacttccgt
    14401 atgtacagcg atgcacgcaa ttcataatcc aaagagcaag ggtgaaccgt tgcatcgcaa
    14461 cagaaaagca tggcaaagag cggtttacat gttctttctt gtcttgatac aagaaagaac
    14521 caaagaaaat caaggctgaa aagcccgacc cgatgggtgc cccgcgggtg ggactgccac
    14581 gcgatactat tcgccacgcc tgcggcgtgg ctcatgtggt atcgcttact agacccatcg
    14641 ccacgttcca ctcccgaccc attagcgggt ctggctttta tgccattgcc tgcttcttcc
    14701 tctccattcc ctctcagggg aaaggactgg ggtaaagccg ttttctaaat ttaacctaga
    14761 cgacccctct aaatctcccc tgaaagggga gactttaacg gtgatcccct ctcctgccag
    14821 gagaggggtt tggggtgagg tcattttcca cgagcaacaa acaacgaact acgtctccag
    14881 ccccgtaggg gcgtattacc aatgtaaagt tcagcaatcg attgcatggc ttttacctca
    14941 aatctatttg aaattcttca taaatctacc gttaataata cctttgctat cttatgaaat
    15001 tgttaattgc agtgtttaaa agattttttt tcctattagg gttaccgctt ttagtactga
    15061 ctgggtacgc acaaggagta gttgagaaca atccgctgtt cgatgatagt gaggttgcac
    15121 gtatcgatat cacaattaat cctacatact tacaaagtat actgcaggct ggaaatgaag
    15181 agagtaacac tgaataccct gccactttca gcttcacttc atcgaaattt acggccgtag
    15241 tggagaatgc tggtttcagg ttaaggggta atacctcgcg ctacgcccaa aagaaatcgt
    15301 ttaaggtatc gtttaatacc tttgaacctg gaaataagtt gaagggtatt gaaaaattga
    15361 acctaaacgg tgaacataac gacccatcga ttgttcgctc caagcttgtt tgggatttaa
    15421 tgggtgaaat tggcttacca gctccacggg caaaccatgt taggctttac attaacaatc
    15481 agtattacgg gctatacatc aacgttgaac atatagatga gaattttgtg aaagcacggt
    15541 ttggcaacaa tgatggaaat ctttataaat gtctatatcc ggccgattta acataccgga
    15601 gcagtgaccc taatgcctat aaatttgttc aaaacggata ccgtgtatat gatctaaaaa
    15661 ccaataccga acaggacaac tattccgatc ttgcacaact tattgatatt attaaccgca
    15721 catccatatc ggatttgcca gctaaacttg agccaatatt tgatgttaat aatttcctga
    15781 aatacttagc agttgaggcg ttaacgggca actgggatgg atactcatac aacaagaaca
    15841 acttttacct ttacagaaat acagccaccg gacgctttga gttcataccc tacgataccg
    15901 ataacacatt tggaattgac tggttcaata ttgactgggc aacacgcaac gttaccacat
    15961 gggtaagcag ccaggcgcga cctttaacaa aaaacatact tgcagttgat gtttaccgca
    16021 agcgctacta cttttaccta aagcagctaa taaacggagc attttcaact aattccattc
    16081 agtccaaggc tattgctcta cgatcaaaaa ttgaagccta cgccactgaa gatccttttc
    16141 gcccactcga ttacggatgg agtagttccg atttctatag ctcctacttc aatgcacttg
    16201 gcgggcatgt taagtacggt ttaatccctt acgtaaccaa gcgcatacag tacgcaaact
    16261 ctcagtttag tatcggtgat attccgccaa ttgtttccaa tgttagctgg ttagcttacg
    16321 gttacaaagt tccagttaca gtatttgctg atgttgacga tgaagagaag gctaacataa
    16381 aactatactt caaagctgat gatagcaatt ggcaaagctt tgaaatgcaa aacaccaacc
    16441 gcaaccacta catttccact ttggggccat ggagcaaacc tgttaaagct atcagctttt
    16501 accttgaatg caccgataag agcggtaaaa caacccgcga gccactcatc ggtgagtatt
    16561 cagtaaaatt caatcaaatt gcaataccca tttgcattaa cgaggtgatg acatccaacc
    16621 aatccaccta tcccgatgag tatggcaact actccgactg ggttgagctt tacaactacg
    16681 gcaaaaatcc catatcgcta cagggcatga gcataagcga tagcttgggt aaacccggga
    16741 aatgggcttt gcccagcatc actattaacc ctggcgagtt tctgcttctt tgggccgatg
    16801 gtgaacccga aaaaggcaat aaccacttac ctttccgact cagtaaccaa ggcgaagaaa
    16861 ttgggctttt tacatctaaa accgatgggt acaagctgat tgatggttac caatttggca
    16921 aaatagccaa aaatgaaagt tttggctact accccaatgc tgtcggcttg cctcaagcac
    16981 tgcttagccc aacacccgga aaaagtaatg tgtacacatc caaacggatt gatatggtgt
    17041 tacctgaaat tattgcctac cccaacccat ttactgaatc tgttacaatt accctatcgt
    17101 atcaaccggc ctacgactac actatttcaa tagttaacct tcaaggaatt accgtgctgg
    17161 agcattacat gaaaaaggat gataacccaa agctaacctg gcgcccacaa aacctttcaa
    17221 agggtacata cttaatagtt tttacaattc acggcaagca aaatcaactt attaaaccgc
    17281 aaaaagttat ttttgaaaaa ttttgaattg atgatacgga ataaactggc attgattttt
    17341 gcatctactt tactaatagt cacaaatgct agctcgcagg ttgctcgcac attatggagc
    17401 ggtgctgaac tccagcttcc cttttcaaaa gcatttcgtg ttacattctc ccctgagcta
    17461 aggctaaccg acgaaaagag aattgatgat tttattcttg aagccgaggc tcaatttcgt
    17521 atccacaaac tcctgcagcc tactttttca tacaggtatt acaggtttta cgatgatccg
    17581 ggtgaatact ttaatggtca acggttagga gcaggtataa actcatcggt tgagttgggt
    17641 cgatttaaac tgaccaatcg catttactac tttcataggc taataaaccg ttacggttat
    17701 ggctactcca ttgaaacaag gcgcgaactc cgcgaaggta tcaaaataac atacaacata
    17761 aggagaacaa agcttgaacc ctttgtgcag gccgaaatat actacgatat ttcacccgac
    17821 cgcaaccatg aattttcaag ggtcagaatc agaaccggtt tggaataccc tttctcaaag
    17881 cggtcgagta tagacctgtt tttccaaatg caggataagc tgaatacaag caacccactc
    17941 cgaacctaca ctattggtat tttttacaac tataaactac ctacgccaaa acccaaatcg
    18001 gttgatatag agtaaatgta ttattaacca ttttaatgta ttacctttgc agcaatttta
    18061 ttgctgtttt acaatgattt cggttaataa tcttacgctt agctttggtg ggttcgacct
    18121 atttaagggg ataagctttc agctaaacga taatgagcat gttgggcttg ttggaaagaa
    18181 tggagcaggt aaaaccacat tgctaaggct aattgctggc gaactacaac ccacaaaggg
    18241 aagtatctca ttacccagtg atattaggtt agggtatttg ccccaacaaa tgaagctggc
    18301 ctcgggaaaa accgtaatag aggaaacccg caaggctttc gccgaaacaa ttcaaataaa
    18361 aaatgacatt aaccacatct cggagcaaat ttcacaccgc accgattacg aaagcaagga
    18421 gtaccttgag cttatcaata agctcaccga attaaacgac cgctatgcca tacttgaagg
    18481 cgatagcgcc gatgcacggg cagagcaaac ccttttgggc ttaggtttta aacgagccga
    18541 ttttagccgt caaaccactg agtttagcgg gggatggcga atgcgtattg agctggctaa
    18601 aatattactc cagaacccac aggttttgct cctcgacgag ccaaccaatc atttggatat
    18661 tgaatcgata cagtggttgg aggattacct taaagacttt cagggtgccc tactgcttat
    18721 ttcgcacgac cgtgcatttc tggataatgt tacccagcga accattgaaa tctcgcttgg
    18781 caatgtttac gattaccgtg taccctactc gcaatacgtg gagttgcgcc gtgaacggcg
    18841 cgaacagcaa atggccgcct accgcaacca acaaaagctc attgaggata cccaggagtt
    18901 tattgacagg ttccgttaca aggccaccaa ggctgttcag gtgcaatcgc gtattaagat
    18961 gcttgaacga atggatatta ttgaggttga tgaggaagat agtgctaccc tgaatatccg
    19021 tttccccgaa gcaccccgct caggcgatgt ggttattgag gccaaggagg tttcgaagag
    19081 ctttggaaac aagcatgttt tttccgatgt ccattttacc attgaacgcg gccaaaaggt
    19141 agcctttgtt ggccgtaatg gcgaagggaa aactaccatg agccgcataa ttgttggtga
    19201 actcccttac gatggacacc tcaaaattgg tcataatgtt accataggtt actttgccca
    19261 aaatcaggat gagctgctcg atgggagcct cactgtactt gaaaccattg accacgtggc
    19321 tgtaggagat attcgcaccc gtatgcgcga tatacttggc gcattcctct tccgtggtga
    19381 ggatgttgat aaacgggtta gcgtactatc cggaggtgaa cggaaccggc ttgccatggc
    19441 aaagctaatg cttcaaccct acaacctgct tgtactcgac gagcccacca accacctcga
    19501 tatgcgatcg aaggatgttc tgaaaaatgc ccttcaggca tttaatggaa ccctaatcct
    19561 tgtttcgcat gaccgcgact tcctaaacgg gctggtagat aaaatctatg aatttaccga
    19621 tggaaaggta aaagaacacc ttggcggtat atacgatttt ttgcagcgca aaaagttgca
    19681 aaacctaaag gaattggaac gtaaacaggt tgagcaaaaa gcaaaaaact caaccagcgc
    19741 aaactccgaa caaaaaatac agtgggagga acgcaaagaa ctcgaccggc aaatccgcaa
    19801 gctggagtcg caggttgaga agctggaaaa ggaaatcgga atgctggaaa gcactattgc
    19861 cgaaaccgat actaagctat cgaaccctac agcccatggc attgacctat ccgataagga
    19921 ttttttcaaa aaatatgaaa gccataagac tgaacttatg ggaaaaatgg agcagtggga
    19981 aaatctactt gctcaagttg aagaactaaa aacaaaacga aacaacacaa actaacaatg
    20041 gaaaactcgg agaacctaat atttggcata agagccgttt tggaagcact taatgctgga
    20101 gcaaaaatta gcaaagtgat agtacgccaa gggcttaccg gcaacctagc atcagaactt
    20161 atgggattgc taaagcataa taacattccc tttcagtatc tacccaacga ggcttttact
    20221 aggtataagg ataaaaatca tcagggggtt attgctttta cctcaccaat tgaggtttat
    20281 agggttgaag atattttacc tcaactacag gaaaaaacca actcgccatt cctacttatc
    20341 ctcgacagca ttaccgatgt acgcaacttt ggtgcaattg ttcgaactgc cgaatgcgca
    20401 ggcgttgatg ccattattgt tccagccaaa ggagccgcca atattggcgg cgatgccata
    20461 aaaacctcgg ctggcgcact atacaacatc cccatttgta gggtaggtag cctaaaaatg
    20521 ctaagctatc agctacgcga ttatggttat aggattgttt cggcaagcga aaggggcgaa
    20581 aagttgctct atgaagctaa ccttaccggg ccaattgcta ttgttatggg agcagaggat
    20641 acaggcatac accccgaagt gctaaggcta tcgcatgaaa ttgttcgtat accattaaag
    20701 ggtaaaattg agtcgctaaa tgtttcggtg gccactggca ttgttctgtt tgaagccgtt
    20761 aggcagcgaa ccctataacc ccttttcgtc cagcaccttt gcgcattgta agcgtccgta
    20821 ctcaatcagc tctgcagcct tgtaaaattc aaaaacggta gccgaatttt ttgatatggg
    20881 aacaagcaca tcgggcgggg ttacctttat tgcatactgg gttaactgcg actgcatgag
    20941 ctgcaccgaa cgcatggcta aatcgaagta gccaatactt tttggcgatt gcttagcttt
    21001 tacctcgctt tccttagctg ccgcattgtg cccaaagaat ttatcccact tggcaacaag
    21061 ttgctcaatt ttagggttat ccttaaccca gctgtttttg gattgcttat tggttggcac
    21121 agccagatcg taagggataa tggcattcaa atcaacagcc accagtaaat cgtttttagt
    21181 tcgcctaaca tactccaggg gcaatggcga aagcactccc ccatcaacaa gtaatgtacc
    21241 attgtatggc aatggtgtga aaacgcttgg gatggaaatg gatgcgcgaa tggcggtttg
    21301 gagcttgcca gaattaaaaa caataagttg attacttaaa atgtcgactg caacagcagt
    21361 gtatggtaga ggcaaatcct ctatgtttac atcaggaacc atattgagtt cctgcatctt
    21421 ttcaaaaacc ttttgccctt taacaatacc ttttgatgag aatgtaaaat cgatcagcgt
    21481 taggatatca attttatcga gcttaagtat ccagtttttg taatcatcaa gcctgttggc
    21541 agccagcaag cctcccacaa gtgcccccat tgatgagcct gagatagatg taatactata
    21601 accccttcgg gttaactcct caatcacccc aatatgggct aaaccccgag cgccaccact
    21661 ccccagtacc agtgctacat tctttgattt aaccatagcc aattgcaaca attatttgaa
    21721 taccaatgtt tttactcaaa gaatgctaat aaaggcaaaa ttgaggaaca accgattaat
    21781 actttacctt gttctcctta ttatcccact ccctaaaggg tacaacaccc ttttcgggac
    21841 taacatgaat gaatgggata cgcctgcggt cgaacgatag gtttacctca tcgtaaataa
    21901 agctatcgtc gaaaccgtgg tacgatgcca tatggcggtt agctgaatag taaaccttat
    21961 cgagacgagc ccagtaaatg gccccaaagc acatggggca aggctcacat gaggtataga
    22021 gaacacaacc gtttagctga aaggtattca ggttcttaca tgcatcgcgt atggccatta
    22081 cctcagcgtg agcggttggg tcgtttgttg atgtaacatg gttattgcca cgccctacaa
    22141 tttttccatc cttaaccaca atagcgccaa aagggcctcc atcgccagcc attacgccat
    22201 caacggctaa ccttatggcc tcgtccataa atttttcgtg ttcgctcata gcttactttt
    22261 aaatctctaa tttactaaga aaatattaaa aggcaattat accaaatatt tattgaaaca
    22321 gaagcgaaac ccaatggttg gattccgctt ccggaagcta attagttacc tttagcaatt
    22381 gctctaatat catcgctcga cattcgctga aaaaccttta aatcaaaaac aggaagcgca
    22441 gccatgatat ggtcaagaat ttccgattgt ataatttcat actgaacaaa tcgtttttcc
    22501 ttgaggaagc agtatatctc catgggaata ccatactcaa caggctgttt cagcctaaca
    22561 acaagggtaa ggtgtttatt aacctcaggg tgatggttta ggtattcctg aacatattgc
    22621 ctgaaaaggt tcaggttggt ttgcgctccg gcattcatca actgaatggg gtcggtttca
    22681 tgcagcttgt caacatcaat gtccttaagc ttgctggcta tcaggtttat attcgacagt
    22741 ttctgtataa gttccttatc gcagaactta acggttttgg agtcaataat aatcgatttc
    22801 attaccctac ggccagccga ctcctccatg ccacgccagt tcttgaagct ctcagtgatt
    22861 agcgcaaagg gcggaacagt agaaatggta ttgtcaaagt tctgaacctt tactgtggta
    22921 aggctaatat caactacagt tccatcgagg tttcttccgg gcatctcaat ccaatcgcca
    22981 agctttagca tctcattagc cgatagctgg attgatgcaa ccaaaccagt tattacatcc
    23041 ttgaatacca acatcaaaac ggcggttgag gctcctaaac ctaccagaat cgatgccgga
    23101 tttttattga ttattattcc tataatcaaa atgcctgcaa aaacataaat aataattttt
    23161 agaacctgca ggtatccttt aattggtcta ttcttggcat atggtaattc ctggtaaatt
    23221 tcgttaactg tgtcgagaaa agcattaaat acactaatgg caataaaaat catgtagatt
    23281 tttgtaagtg cctgtaaaaa cagtgtaacc ttgggtgaat agtcgtacag ggctatacca
    23341 agggtcaagt acaccacaat ggcaggagca aagtgcgcta aacggtgaaa gaatcgtttt
    23401 tcaaccaaaa catcgtccca gtcggttttg gttcgcctag ctacactagt catggtggtt
    23461 aaaaaaatcc gctttgcaat aaaatcggca attaatgcaa gtataaaaat ggctgcaagc
    23521 gatattgcca ccttgaaaaa ctgggcaaag gtttcattta taccttggct taggagccac
    23581 gactcaaggt cctcgagaaa aagatacttg cgaattactt gttcctccat accttccata
    23641 ttcaagtatt ttttgttaat agtttactaa aggcaaagtt acagctaaaa aggtaaacca
    23701 tcgtcgctcc ccccctccat gggtggataa agattatcgt tgaacggagg gggttctggg
    23761 gcttgttcgg gtgtggcacc cttgctcatt ggttcaattc gccatgccct aacatcggtg
    23821 taccaccgac cgttgaactc gcgcgattca atgttgattg cgacttttat ttgactgccc
    23881 tcctgaattg gatcaagctc tctcacctta tcgccccaaa gcgatatgca aatctttcgg
    23941 gggtactgct cctgcgtttc aataatgaat tcctgcttga cccaagcgcc atttttccca
    24001 tgcccggttt gctggtcgag ctttttaata agtttacctt ggatttccat ttttcaatcg
    24061 attaaaaaac cgggcgaatg atcaacccgg ttatgtatga ttgactataa ttaaactact
    24121 tcttgctgtc ttgagcattt tcctttacaa tgtcaagcag ttcaacctca aaaatcaggg
    24181 cttggttagg gccaattggg cttcccggtc gaaccctttc cccgtaacct aactcactag
    24241 ggatgtaaag aatgaactta gagcctactt tcattttttg caaaccaata gtccagccag
    24301 gaataacctg gttgagttta aactgggcgg gttctcccct gtcgtacgat gaatcgaatt
    24361 gggttccatc aatcagagta cccttgtaat gaacctttac cacatcctca gctgtaggca
    24421 taagccctgt accttcctca ataatcttat actgcaaacc cgatgagtca acaactacac
    24481 cctccttggt tttgttctca tcaaggaact tgttgcttgc ctc
//
LOCUS       DAOSAL010000033        24494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_1482_length_24494_cov_51.992594, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000033 DAOSAL010000000
VERSION     DAOSAL010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 24494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24494
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1482_length_24494_cov_51.992594"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            270..497
                     /locus_tag="PK049_06220"
     CDS             270..497
                     /locus_tag="PK049_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61189.1"
                     /translation="MKKIRTFNDEIERPERLCETCIYYIKNRTCVAYLDRIPEKYWKQ
                     KVGEKPLHDSPKDNPDYDNIFYKEKYIGKLE"
     gene            709..1845
                     /locus_tag="PK049_06225"
     CDS             709..1845
                     /locus_tag="PK049_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOK61190.1"
                     /translation="MARVKDINPFLVNSYISKEYFCNREDELNVLLRNVRGNVNTTLI
                     SPRRLGKTGLILRFFEMLGEMGDYGTVYVDIYSSRNLSDFIKLLTTAIINKFPERTPV
                     GKRFLKLLKGLRPVFSFDAITGAPQVQFNYQSESDKEYTLQQLLAFINEQPHPVVVAI
                     DEFQQIAHYPEKNVEAILRTYIQQFNRLCFIFSGSRRHTLMDIFANANRPFYSSTRFI
                     NLDAIERVTYRNFIREKFEQGGRAIDDESIDYVLEWTKGYTFYTQSLCNRLYSLRKIN
                     LDAVKAECLALLRENEPIYLQFRNLITSKQWEFLLALAKEESVSQIYSKEFLVKHQLG
                     TPSSIHRMLDALLEKEMILESVSSTGSSYSVYDVFLMRWMQRTY"
     gene            complement(1934..2836)
                     /locus_tag="PK049_06230"
     CDS             complement(1934..2836)
                     /locus_tag="PK049_06230"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HOK61191.1"
                     /translation="MSTIAITDCEGNVLYSLEECEMFPNYTAVLKDAIAQGKDLTGLC
                     IKNQNISNVVFQGTTLYNAVFENCNFHKVEFIECQADSVWFNRCNLDGLKVKKTNLSK
                     AQIHKCSISKAHFTRSNLNDSLIFDSNLHLAVFLKTQLNNVGFDNCNLEKAKFEECSL
                     AEAGFYNLSPTYQWINDVLFSRCILNNCNLSSLNSLSMLFIRESNIEDAELQDQNFTR
                     VINTYTKVLYAIESDTVWWEDFRGNFEQFKDMVENDTMENGTKYYLDDFLYDELSKVV
                     YYLEQWHNEQTIIAQNTLSINKCE"
     gene            3229..5244
                     /locus_tag="PK049_06235"
     CDS             3229..5244
                     /locus_tag="PK049_06235"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006044650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HOK61192.1"
                     /translation="MTLEEFRKDILQGIPDELPAPKPWDPSVNHAPKRKKILTLEEKK
                     LAVKNALRYFPPKHHAVLAEEFARELEEYGRIYMYRFRPDYKIYARSINDFPHKSVQA
                     ASIMLMLSNNLDYAVAQHPHELITYGGNGAVFQNWAQYRLTMKYLAEMTDEQTLVLYS
                     GHPMGLFPSHPDAPRVVVTNGMMIPNYSSKDDWERYNALGVTQYGQMTAGSFMYIGPQ
                     GIVHGTTITVMNAGRKQMKPGETDLRGKIFVSSGLGGMSGAQPKAAVVAGMVGVIAEI
                     NPKAVEVRHGQKWVDEVYSSLDELIPRIREASANKEAVSLAYHGNIVDLWERLVEENI
                     HVDLGSDQTSLHNPFSGGYYPVGLSFEEANRMMAEQPELFKEKVYESLRRHVNAINKM
                     TERGMYFFDYGNAFLLESSRAGADILKPNGDFRYPSYVQDIMGPMFFDYGFGPFRWVC
                     TSSDPKDLATTDAIALEVLEEIAKNAPAEIMGQLQDNIHWIREAGKNNLVVGSQARIL
                     YADAEGRIKIAAAFNKAIRDGRISAPVVLGRDHHDVSGTDSPYRETSNIYDGSRFTAD
                     MAIHNVIGDAFRGATWVSIHNGGGVGWGEVINGGFGLTLDGSDAADRRLHMMLHWDVN
                     NGIARRSWARNSGAMLAIKREMERTPLLKVTLPNIADDSLIDKVVKG"
     gene            5273..5677
                     /locus_tag="PK049_06240"
     CDS             5273..5677
                     /locus_tag="PK049_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61193.1"
                     /translation="MKRTAQLLLFIATFVLLSGSSCDKYEKGEGITGVWRCRENYQGT
                     KFRSYNVSVDRYTQLDSSTYVIYNLYNLGLDVETYVQLKDTVFTVLSTNSDLYFITGR
                     GVWHRYSQTIDWEYSVSGQVNDPFVSATFDRP"
     gene            complement(5674..6003)
                     /locus_tag="PK049_06245"
     CDS             complement(5674..6003)
                     /locus_tag="PK049_06245"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOK61194.1"
                     /translation="MVKVIQNESDLSKALSEPASAIYFNSQACGVCHVMLPQLEQIVG
                     SEFPKMHIYVVNASETPEICGKFSVFTFPTVLIFFEGKESLRMARNINLATFRQAVAR
                     PYSILFD"
     gene            complement(5997..6386)
                     /locus_tag="PK049_06250"
     CDS             complement(5997..6386)
                     /locus_tag="PK049_06250"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOK61195.1"
                     /translation="MEVGNDILPKTLVLGASQKPERYSYLAIRSLRKHGIEVVAIGAR
                     KGMVDDIEIHTELQDIPDIHTITLYLSARNQAPYYNYIINLKPKRLIFNPGTENPELE
                     KLAREHGIDVVEGCTLVMLSNGTYYEW"
     gene            complement(6391..6870)
                     /gene="ispF"
                     /locus_tag="PK049_06255"
     CDS             complement(6391..6870)
                     /gene="ispF"
                     /locus_tag="PK049_06255"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007749445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOK61196.1"
                     /translation="MDFRIGFGYDVHQLQQGLPFWLGGVQIEHTKGSLGHSDGDALIH
                     AICDAMLGAANLGDIGVHFPDTDPSLKGIDSKILLRKTMDIIAQIGYTIGNIDSTICL
                     QRPKVKDYIPKMQAELAKALDVEPQQISIKATTTEKLGFVGREEGVTAYAVVLLRKN"
     gene            complement(6870..8063)
                     /gene="porV"
                     /locus_tag="PK049_06260"
     CDS             complement(6870..8063)
                     /gene="porV"
                     /locus_tag="PK049_06260"
                     /inference="COORDINATES: protein motif:HMM:NF033710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HOK61197.1"
                     /translation="MIKKFFGIATILLVGLQPTVKAQFDSTISLSGGLNALRIAVPFL
                     TIAPDSRSAALGDAGVASVPDVNSQHWNPAKFPFIEGKKWGMGFSYTPWLRNLVNDIN
                     LTHLTGYYQFDKMQSISGSLLYFSMGEITFRDNNNNYIQAFNPNEFAIDFAYSRKFSD
                     HIAGAMAFRYIRSDLTGNFTTTGTGSGKPGTSFAADLAVYYQNAINLAGKSGEMAIGG
                     NISNIGTKLSYNEDPQKEFIPINLRVGGRLSLNIDQYNGFSYLLDLNKLLVPTPPVYN
                     SQDEIVSGMDPNVSVVQGMIQSFYDAPGGFKEEMQEIYIATGVEYWYARQFAIRAGYF
                     HESQNKGNRKYITLGAGLRYNVFNIDFSYLIPTGGFNSPMANTLRFSISFEFEPARTR
                     TNRNK"
     gene            complement(8153..11905)
                     /gene="porU"
                     /locus_tag="PK049_06265"
     CDS             complement(8153..11905)
                     /gene="porU"
                     /locus_tag="PK049_06265"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HOK61198.1"
                     /translation="MRAVLFIILLTTQALGAFTQEFHRILEWVETDKKNINCLWFNGE
                     DFPDPASKVPYYTELIPIPDSKEYRIEITHIKWGKLNRDFDSSIVQTEPNPTTKSLRV
                     GANRVLLFSIPAMRNNASGSIEKLESFSFRIEPISSHEKNIDKTIRTSLSNSVLASGK
                     WVKVGVQKSGIYRISFEDLQRYGFSNPENISIWGIGGKSLPLMNAQSNLDEVAPIPIQ
                     ISKGADGIFNQGDYILFYAQGPETLTYNETYSAWKWEKHGYTKTIYYFLTTSQPQSII
                     GFADTPTETENYATNEFDQVTTFESNDTNLVKSGREWFGEIFDIKTTYSYNVSTPLPV
                     NNSTYKVWVRVAARSSSTSTFTLTNNGTLGSITLNSVIIGDELANVVSVNEKVFSGNT
                     VGQNLTFDLTYNKPNSGSVGFLDFISIQSRHGLSYSNAQLPFQDFQSVGAGRISRFTI
                     SNANSSVQVWDITDINEAKRVNISYANGTITFKQATNTLKTFIAFEPDKAYSVVGYAN
                     VDNQNIRGSGFFDYFIVTHPSFKTYAEELVQLHRQRSNLSVAVYTTDQVYNEFSGGNP
                     DVAAIRNMLRYFYKAATNDDDKPKYLLLFGDGSFDNLSNSQVNTNFIPTYQSPRSINR
                     VESFVSDDFFGLLDDNEGEASGYVDIGIGRLPVSSPEQAANLIAKIKSYMENEDIDDW
                     QSKLVFIGDDEDGNVHMQDANTLAKYIETNYPAYNIQKIFFDAYQQEVVSGGQRYPDV
                     TSAINSAVNNGALLLNYTGHGNERWLAHEKVLMLNDVLGWKNAKRLPLFVTATCEFSR
                     FDDYHMTSTGEWILLTPKGGAIALLSTTRLVYSSPNFTLNYNFIQQLFKTDANGNYLR
                     LGDLVRITKILSGTGYNKRNFTLLGDPALMLKYPTYNIKISYINERPASEPADTLKAL
                     NKVNLKGFIADNLDTTVENFNGTVSVTLYDKEREITTLANDGGTPMTFKSRDNIIFKG
                     KATVSQGTFSIDFTVPKDINFSYGNGKISLFATDGMQTALGVHDSIVVGGIGNPESID
                     SEGPQIKIFLNDTKFTNGGICNSNPLLLVYLSDASGINTTGAGIGHDLTATLTYPAGQ
                     SETFNLNNFYKANTDDYTQGIATYQFSGLTPGTYTIEVKAWDAFNNSNTGKIVFRVIG
                     DDKLVVGNFYCVPNPVKDNTQFYFETNTNETELDVKLEIFNINGSKVAELLKKRIYTE
                     GYRIGPLEWNGCNSNGEKLAKGIYFARIQVSSVQGEQVATTKFVVL"
     gene            12009..13046
                     /locus_tag="PK049_06270"
     CDS             12009..13046
                     /locus_tag="PK049_06270"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HOK61199.1"
                     /translation="MISKNSFFLLYLLGLVLCFEKSHGQDPVFSQVFFNPTMLNPSYA
                     GASKDMRLGIIYRNQWTMIDMPYSTFGVSFDRRIDFGPSWKHSFRNNRSQSSGRSGFG
                     INIMSDVEGKGTFSRNTLDLIYSYGIQPTYNSHVRFGLQTSAIFRTRSFSGLVFPDMI
                     DPAGNVIGNSDAVGYTTWNYDIGLGVAGDFENFYGGFAVHHLLQPVEASFSGGKAYIP
                     RKYTFHIGADFNLYKWRRFKEVLLFSPNVIYIQQLNFNQLNLGFYLSKNWAVAGLWFR
                     ENLSLNSHSFVVTLGYADYMFRIGYSYDFSIMQHGLRGLPTSSHEVTLGWNFEYKRGK
                     KKYRYIKCPKF"
     gene            13129..14661
                     /locus_tag="PK049_06275"
     CDS             13129..14661
                     /locus_tag="PK049_06275"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOK61200.1"
                     /translation="MSLKYRILLLASAGIFGLNSCGLFGGGGGGTGASEKTGWEYNSP
                     DNGGFEVVENWRPEAGPGLVFIEGGTFTMGRVEQDVMYDWNAKPRRVTVTSFYMDETE
                     ISNVDWREYVHWIKRVYSTYPQVIKNALPDTLVWRSPLGYNEPFVTDYFRHPAYNDYP
                     VVGVNWLQANDYCIWRTDRVNERLLVDKGIIEFDLAQKGADAFSTEAYLSGQYDARVK
                     KLLPSLDPEKPEGRHVRFEDGILLPKYRLPTEAEWEFAASGLVGNTYDERMVERRIYP
                     WNGHNVRNPESKVRGQMMANFVRGRGDYMGLAGNLNDENTFPGPVKSYWPNDYGLYCM
                     AGNVNEWVLDVYRPLSFEDVDEIRPFRGNVFKTPYDLDGNYSKKDSLGRIPYREVTEE
                     EAANRQNYNKAYYINYKDGDVASTLDFRDGQTVKEKNSDRMYYQGDTQQKNVRMTTLI
                     NDHVRVYKGGSWKDRAYWLSPGTRRFLDEASSTNDIGFRCAMEAVGAQSAKKRGERIG
                     GK"
     gene            14729..16012
                     /gene="murF"
                     /locus_tag="PK049_06280"
     CDS             14729..16012
                     /gene="murF"
                     /locus_tag="PK049_06280"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOK61201.1"
                     /translation="MIEHIYKEFEKSYHVTIDSRKVKAGSIFFALKGENNDGNAYAAN
                     ALQDGAALAVIDNPHYMTKGCVLVENALNALQQLANYHRQKINAQIFAITGSNGKTTT
                     KELLYTVLSQKFKTYATVGNLNNHIGVPLTLLSITPDVDFAIIEMGANHPGDISELCR
                     IAEPDFGLITNIGKAHLGGFGGFEGVKNTKGELYNFLALRDRAIFYNHQNPILVELLN
                     KYKANNVIPYSKYFEDFIVVEKPESPFLSISISEGGKLTTILTNLVGQYNVENVMAAY
                     AVGRHFGVGPGTIKDAIESYIPSNNRSQLLKTQNNTVIMDAYNANPTSMENALKNLAK
                     LHASKKVAILGEMLELGEYSLDEHKKIVELASALNFDKILLVGKGFSSFNGMFYYFDN
                     SSECAEYLKQNPIKDSMVLVKGSRGVKLEIVVEAL"
     assembly_gap    16106..16205
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(16296..16550)
                     /locus_tag="PK049_06285"
     CDS             complement(16296..16550)
                     /locus_tag="PK049_06285"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOK61202.1"
                     /translation="MHYTYIIYSSSTDRFYIGSTSDEISQRIRRHNAPHRGFTSMAND
                     WRLVYLEQFDSIQQARKRERQIKAWKSRKRVEELCGFSKP"
     gene            complement(16585..16659)
                     /locus_tag="PK049_06290"
     tRNA            complement(16585..16659)
                     /locus_tag="PK049_06290"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16622..16624),aa:Val,seq:tac)
     gene            complement(17148..18431)
                     /locus_tag="PK049_06295"
     CDS             complement(17148..18431)
                     /locus_tag="PK049_06295"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOK61203.1"
                     /translation="MTYNEALDYIYSHLPIFQRVGKAAYKADLGNTLKLDEYFGHPHR
                     TFKTIHIAGTNGKGSTSHMLAAVLQKAGYRVGLYTSPHLRDFRERIRVNGEMIPEDEV
                     AKFISQHKDFFDQIMPSFFEMTVALAFDYFSRMNLDVAVIEVGLGGRLDSTNIINPIL
                     SVITNIGFDHTDLLGDTLPKIASEKAGIIKPDTPVVVSKYQPEVENVFIAKAKEMNAP
                     IYFADQEIQVYEQYIDTTGLQVLKLADKVRDIKIEIDLLGNYQRYNISGVLKSIEILS
                     GKGFLLSNEALMEGLRNVQTLTGLMGRWQKLSENPLIYCDTGHNVDGIKYVVEQLRHI
                     PHNNLHMVIGVVADKNIDGMLALLPKNATYYFTQAAIPRALNHEELKKRAAEHQLYGH
                     SYSTVALALAAAKEASNPNDLIFVGGSTFVVAEVV"
     gene            18571..19899
                     /locus_tag="PK049_06300"
     CDS             18571..19899
                     /locus_tag="PK049_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOK61204.1"
                     /translation="MAKSKVNKIFVIDTNVILHDFQCLYKFQDNDIVIPIVVLEELDK
                     FKKGNEMINYHAREFVRELDKIAGDKLFNGGLPLGKNLGKLRIETGKPMPPEMKESFA
                     EFTPDNRILAITIHLVKHNPERPVILVTKDINLRMKAKSLGIVAQDYLNDKVEDIDSL
                     KKEIEIIEKVDDALIQKLYNSEEKLLAKDLKLKPYANQYYILKGNKSSALAHYDKATD
                     TLDRVEKVRAYGIDPRNAEQTFALDALLRPDIQLVALTGKAGTGKTLLALAAALQQEE
                     QFDQILLARPIVALSNKDIGFLPGDVDEKIGPYMQPLFDNLGVIKNRFKPTSKDYMHI
                     EEMQRADKLVITPLAYIRGRSLSNVFFIVDEAQNLTPHEIKTIITRAGEGTKFVFTGD
                     IHQIDHPYLDMKSNGLTYLSDRMHGQEIFAHINLVKGERSYLAELASDLL"
     gene            19917..20405
                     /locus_tag="PK049_06305"
     CDS             19917..20405
                     /locus_tag="PK049_06305"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOK61205.1"
                     /translation="MAKTLLILRHAKSSWAEADKADKDRALKAKGISDIVNTARAIAP
                     KVQGLDLIITSSANRAVHTAILFAETIGFPHDKIRIEPMVYQADERELVEMIKSLPDE
                     YKSVMVVGHNPTLTYLVNTFVANRIHELPTSGLVGVKFDINSWIELSAEKVYSVFQSF
                     DW"
     gene            20482..22566
                     /locus_tag="PK049_06310"
     CDS             20482..22566
                     /locus_tag="PK049_06310"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HOK61206.1"
                     /translation="MAPKKIPLVNRELSWLTFNGRVLQEAANPSVPLIERIRFLGIFS
                     NNLDEFFRVRVATLRRLLVVEKGAKKIMGDNPRKILEKIQRIVIDYQKKFDNTFQEII
                     DELRKENIYLINETQLSAGQIDYLNGFFDDHISEFVAPVILKRNNKFPELVDQSIYLA
                     VKLTNSQKPNTKEFALIEIPSRFVKRFIQLPNEGDKSFIILLDDVIRLCLSKVFKILP
                     FDTFEAYTVKITRDAELDVDNDISESLLEKISKGVKNRKRGEPVRLVYDEKIPTDLLK
                     FIVKGLNLDEDDYVIPGGRYHNFKDFIRFPSLGRNHLINLPLEPVSVKELDEATRIMD
                     IMSDHDFMLHYPFHSFQYYIRLLREAALDPAVKSIHITLYRVATESRVVHALINAAQN
                     GKKVTAIVELRARFDENANIYWSKQMQEAGVQVIFGVPGLKVHSKMTLITRQEKNKER
                     KYAVVSTGNFHEGNANIYTDVNLFTADSRITSEILKVFTFLETNYKTFSYKHLLVAPF
                     NMRRRLYSLIDTEIENAAKGLPAYLIGKINNLVDEEMIKKLYQASKAGVKIKLVVRGT
                     CALVPGVKGISENIEVYGIVDRFLEHSRIFIFANGGNEVCYISSADWMTRNLDYRIEV
                     ATPIYSKPLIEEMKTVVNYALRDNVKARLVNHGGCNEFKPCKEGEPEFRSQLELYSFY
                     QNIERHKNGEVK"
     gene            22563..23468
                     /locus_tag="PK049_06315"
     CDS             22563..23468
                     /locus_tag="PK049_06315"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HOK61207.1"
                     /translation="MKILKFAAIDIGSNAARLLLTNVVEDGETVVFSKSSLVRVPIRL
                     GEDAFSIGYISEHRAQKLLKTMIAFKNLMEAQDVVAYRACATSAMRSSSNAPELVSMV
                     KQHANINIEIIDGSREAEIIYANGIAEMLDHDRPYIYVDVGGGSTEITVFEKGEIKAS
                     NSFNIGTIRILKGMIGKPDFDQVRDWIKSIVKKKNQPKIIGSGGNINKLYKLARKPIG
                     EPLYHKEVKNLYDFLDSYSVDDRIKLLGLNPDRADVIIPATKIFLKVMKWTGAKEVIV
                     PTIGISDGIIRTLYADYKQSLALQQ"
     gene            23549..>24494
                     /locus_tag="PK049_06320"
     CDS             23549..>24494
                     /locus_tag="PK049_06320"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HOK61208.1"
                     /translation="MNQGRKRVAFYTLGCKLNFSESSTIARQFADMGYERVSPDSEAD
                     VYVINTCSVTEHADKKCRQAIRKFTTKSPNAMVAVVGCYAQLKPEEIAQIDGVDFVLG
                     AADKFNLPHLIGNDAKKTKVKVYSCDIDSVSDFFPAYSTGDRTRSFLKVQDGCDYHCS
                     YCTIPLARGKSRNMPISQIVDQAKAIASQNVKEIILTGVNTGDFGKSSGETFLELIQQ
                     LDRVEGIERFRISSIEPNLITPEMVDFVAQSAKFLPHFHIPLQSGSNRILALMRRRYR
                     RELFAERIELIRSRMPDTFFGVDVIVGFPGETDDDFSDA"
BASE COUNT         6931 a         4886 c         5349 g         7228 t
ORIGIN      
        1 gttgtcaaga taagaaaagt tatgaccaat atgtcaaaga tacaatctga accgaacttt
       61 ttttgcgagc tcggcgaagc caaagcaatt cacatcacca caaacctaca aaaaattggt
      121 tgaaatgttg tgaccagtat gtcaaagata caatcttaac cgagtgtttt ttgcaagttc
      181 ggctaagcat atgcaattaa caatagaaat gattgaaatt gtagaaatgt tacttaaatt
      241 agacaatcct tacctaaaaa aatagagcca tgaaaaaaat tagaacattt aacgacgaaa
      301 tcgaacgccc cgaaaggctt tgcgaaacgt gcatctatta cattaaaaac cgaacttgtg
      361 ttgcttacct tgaccgcata cccgaaaagt attggaagca aaaggttgga gaaaagccgt
      421 tgcatgacag ccctaaggac aatcccgact atgataacat attttacaag gaaaaataca
      481 ttggaaaact agagtaatga aagggtttga caagttaggg gactacggcg ccagagccgc
      541 taaactatta gtcgcacccc gcgcgggtgc gtggattgaa taagtttggt taacccataa
      601 actttgatta ttaaatacat caatttcgca atatgcgtta tacaaaatgc gttatatatt
      661 ttgcgaaatg tgtttttcta catatattag cataaaataa atggtaaaat ggctagggta
      721 aaggatataa atcccttctt ggttaatagt tatatctcaa aggagtattt ttgcaatcgt
      781 gaggatgagt tgaatgtcct gctgaggaat gtaaggggta atgtgaatac aactttgata
      841 tcgccacgca gattggggaa aacagggcta attttacgct tttttgagat gcttggcgaa
      901 atgggtgatt acggaactgt ttacgtggat atttactcct cacgcaatct ctctgatttc
      961 attaaactgc ttaccactgc aattatcaat aagtttcccg agagaacccc tgtgggaaag
     1021 cgtttcctaa aactgcttaa gggcttacga ccagtattct cgttcgatgc catcactggc
     1081 gcaccgcaag tgcagtttaa ttaccaatcc gagagcgata aggaatacac gctccagcaa
     1141 ttgcttgctt ttataaatga acaacctcat cctgttgtgg ttgccattga cgagtttcag
     1201 caaattgctc attaccccga gaaaaatgtg gaggctatac tccgtacgta tattcaacaa
     1261 tttaaccggc tttgttttat ttttagtggt agtcgaaggc atacccttat ggatattttt
     1321 gccaatgcca atagaccttt ctactccagt acccgtttca tcaaccttga tgctattgaa
     1381 agggtaacgt acagaaattt cattcgtgaa aaatttgaac aagggggaag agctatagat
     1441 gatgaaagca tcgattacgt gctggaatgg acaaagggct atacatttta cacgcagagt
     1501 ctttgcaata ggctatattc gttgagaaaa attaacttgg atgccgtgaa ggcagagtgt
     1561 ttagcgttgc ttagggagaa tgagcccata taccttcagt ttagaaattt aatcaccagt
     1621 aagcaatggg agtttctact tgccctggca aaggaggaat cagtatcaca aatatatagc
     1681 aaggaattcc ttgtcaagca tcagctgggt acaccctcgt ccattcatag gatgctcgat
     1741 gccctccttg agaaagaaat gatacttgaa agcgttagct caacgggatc aagctatagc
     1801 gtgtatgatg tatttttaat gcgttggatg caaagaacat attaacatgt ctgtagcact
     1861 ttatgcggtg tagcaaaggg tatgaccaat atgcaaacga tacaatctga accaatcgtg
     1921 tttagtgcaa ttgctattcg catttattga tgcttagcgt attttgagca ataattgttt
     1981 gttcattatg ccactgctcc aggtaataca caaccttgct taactcgtca tacaaaaagt
     2041 catcaaggta atatttagtt ccgttttcca tcgtgtcgtt ctcaaccata tctttaaact
     2101 gctcaaagtt tcctctaaag tcttcccacc aaaccgtatc ggattcaatg gcatacagca
     2161 ctttagtgta agtattgatt accctggtaa agttttgatc ctgaagttct gcatcttcaa
     2221 tattgctttc ccgaatgaaa agcatcgaga gggagttcag cgaactcagg ttacagttat
     2281 tcaatatgca tcttgagaat aggacatcgt taatccattg gtaggttgga gataaattgt
     2341 aaaatcctgc ttcagccagt gaacactctt caaactttgc tttttctagg ttgcaattat
     2401 caaacccaac attattgagt tgtgtcttaa ggaaaacggc aaggtgtaaa ttactatcaa
     2461 atattaaaga gtcgttaaga tttgacctgg taaagtgtgc cttgctaata ctacatttgt
     2521 gaatttgggc tttggaaagg tttgtctttt tgactttaag cccatcaagg ttgcatctat
     2581 tgaaccaaac ggaatcggct tggcattcaa tgaactcgac cttatgaaag ttacaattct
     2641 cgaacaccgc attgtaaagc gtggttcctt ggaatactac attacttatg ttttggtttt
     2701 taatgcacaa tcccgttagg tctttaccct gtgcaattgc atcttttagt actgcagtgt
     2761 aattggggaa catctcacat tcttcaaggc tatacaaaac gttgccttcg cagtcggtta
     2821 ttgcaattgt gctcatatct tcatctttat tctatctatt ccattttgaa aagtgataat
     2881 tttgggttgt atttaatatc gttgcatttg gaactttaag ctattgcaat aggtaaagta
     2941 ttactaaagt aatgcaattt ataaaaaaca tttgtttaca tcaatggatt aaacgttagt
     3001 tttttttaat acagcaatac cgccctgtta caactggttc cctcccattc cgaataattc
     3061 cgaataattc cggttcattc caccccattc ctctaacttc ctctaacttc ctctaacttc
     3121 ctctaacttc ttctaactcc ttttacatct taataatctt tcccacctcc ctcgcctgaa
     3181 atcgttttaa aattcctacc tttgcggtaa gagtaaaaaa atgtaactat gacacttgaa
     3241 gagttccgta aggatatact gcaggggatt cccgatgagc ttccagcacc caagccatgg
     3301 gatccctcgg ttaaccatgc gcctaagcgc aaaaagattc ttacccttga ggagaagaag
     3361 ctagcggtta agaatgccct taggtatttt ccgccaaagc atcatgctgt tctggccgag
     3421 gagtttgccc gtgagctgga agagtacggt cgtatctaca tgtaccgttt ccggcccgat
     3481 tataagattt atgcacgatc cattaacgat tttccgcata aatcggttca ggctgcatct
     3541 atcatgctaa tgctaagtaa taacctggat tatgctgtgg cgcagcatcc gcatgagctt
     3601 ataacctacg gcgggaatgg ggctgtgttc cagaactggg cgcagtaccg cttaaccatg
     3661 aagtacctgg ccgagatgac cgatgagcaa acccttgttc tttactcagg ccaccccatg
     3721 gggcttttcc catcgcaccc ggatgcaccc cgcgtggtgg ttaccaatgg aatgatgatt
     3781 cccaactact ccagtaagga cgactgggag cgttacaacg cacttggcgt gacccaatac
     3841 gggcagatga cagccggttc attcatgtat attggccctc aaggaattgt gcatggaaca
     3901 accattaccg tgatgaacgc cggtcgtaag cagatgaaac cgggcgaaac cgatttgcgg
     3961 ggtaaaatat ttgtatcatc aggtcttggt ggaatgtcgg gtgctcagcc caaggctgcc
     4021 gtggttgcag gaatggttgg cgttattgcc gagattaacc ccaaggctgt tgaggttcgc
     4081 catggacaaa agtgggtgga tgaggtttac tccagccttg acgagctaat tcccagaatt
     4141 cgtgaggcct ctgccaacaa ggaagcggta tcgctggcct accatgggaa tattgttgac
     4201 ctttgggaac ggttggttga ggagaatatc catgttgatt tggggagcga ccaaacctca
     4261 ctccataacc cattttctgg agggtactac cctgtaggcc ttagctttga ggaggctaac
     4321 aggatgatgg ctgaacagcc agaacttttc aaggaaaagg tttacgagag tcttcgcagg
     4381 catgtaaatg ctattaataa gatgacagag cgtggcatgt actttttcga ctatggcaat
     4441 gcattcctac tggagagtag ccgcgccggt gccgatatcc ttaaacctaa cggcgatttt
     4501 aggtacccgt cctatgttca ggacataatg gggcccatgt tctttgacta cggctttggc
     4561 ccattccgct gggtttgcac ctcgtccgat cctaaggatt tggccaccac cgatgctata
     4621 gcacttgagg tacttgagga gatagccaag aatgctcctg ccgaaatcat ggggcagcta
     4681 caggataaca ttcactggat acgtgaggct ggtaagaaca acctggtggt tggatcacag
     4741 gcccgaatcc tttacgccga tgccgagggc cgaattaaga ttgctgctgc ctttaacaag
     4801 gcaatccgcg acgggcgcat ttcagccccg gtagtgctgg gtcgcgacca ccacgatgta
     4861 tcgggaactg actcgccata ccgcgaaaca tcaaacatct acgatggctc acgcttcact
     4921 gccgatatgg ctatccacaa cgtaattggt gatgctttcc gtggtgccac ctgggtatca
     4981 attcacaacg gaggtggtgt tggctggggc gaggtaataa acggcggctt tggcctcact
     5041 ctcgatggct ccgatgctgc cgatagacgc ctccacatga tgctccactg ggatgtgaac
     5101 aatggcattg cccgtaggag ttgggcgcgt aacagcggcg caatgcttgc cattaagcgc
     5161 gagatggaac gcacccctct gctcaaggtt acgcttccta atattgccga cgatagttta
     5221 attgataagg tggtgaaggg ctaatgccct tcatcactta tatacttgag ttatgaaaag
     5281 gactgcccaa ctgttattgt tcatagcaac ttttgtattg ctatctggtt catcgtgcga
     5341 caagtacgaa aagggggagg ggattaccgg ggtttggcgg tgccgggaga attaccaggg
     5401 aaccaaattc aggtcgtata acgtgagtgt tgaccgctac acccaactcg atagctcaac
     5461 ctacgtgatt tataaccttt acaaccttgg gcttgacgtt gaaacctatg ttcagcttaa
     5521 ggataccgtt tttacggtgc taagcaccaa tagcgatcta tactttataa ctggcagggg
     5581 tgtttggcac aggtacagcc aaacaattga ctgggagtac agtgtttcag ggcaggtaaa
     5641 cgacccattt gttagcgcaa cattcgatcg tccttagtcg aataggatgg agtaggggcg
     5701 agctacggct tgcctgaaag tggccaggtt gatattcctt gccatacgca acgattcttt
     5761 cccttcaaaa aatattaaca cggttggaaa ggtaaatact gagaattttc cgcatatctc
     5821 aggagtttcg ctagcattta ccacgtaaat gtgcatcttg gggaattcac taccaactat
     5881 ttgctcgagt tggggcagca taacatggca aaccccgcat gcctgtgagt taaagtatat
     5941 agcactggct ggctcgctca gggccttact taaatcggac tcgttctgta taacttttac
     6001 cattcgtagt atgttccgtt acttagcatt acaagggtgc acccctccac cacgtcaatt
     6061 ccatgttctc gggcaagttt ttccagctca gggttttcag ttcccgggtt aaaaatcaga
     6121 cgtttgggtt tgaggttgat gatatagttg tagtatggag cctgattacg tgcgctgaga
     6181 tatagtgtta tggtgtgaat atctggtata tcctgtagtt cggtatgaat ctcaatatca
     6241 tcaaccatcc ctttacgtgc accgattgct actacctcaa taccatgttt cctaaggctt
     6301 cgtatggcta ggtacgagta acgctcaggt ttttgactgg cgcctagtac caaagttttt
     6361 ggaagaatat cgtttcctac ctccatacag ctaattcttt ctcagaagaa ctacggcata
     6421 ggccgttacg ccttcctcgc gtccaacaaa accaagtttc tcggtagtag tagcttttat
     6481 tgaaatttgc tgtggctcaa catctaatgc tttggcaagt tcagcctgca ttttgggtat
     6541 ataatccttt acctttgggc gttgtaggca gatggttgag tcgatgtttc caatggtgta
     6601 acctatttgg gcaattatat ccattgtttt ccgtaaaagt attttactat cgataccctt
     6661 taaagaggga tcggtatcgg ggaaatggac acctatatcg cccaggttgg ctgcacccag
     6721 catggcatcg caaatggcat ggataagggc atcgccatcg gagtgaccta atgagccctt
     6781 ggtgtgttca atttgaactc cacctagcca aaaggggagc ccttgctgca gctgatgaac
     6841 atcgtagcca aaacctatcc taaaatccat tacttattcc tatttgttct tgttcttgca
     6901 ggttcaaatt caaaagagat tgaaaagcga agggtattgg ccatagggct attgaaccca
     6961 ccggttggga ttaggtacga gaaatcgata ttaaaaacat tgtaacgcaa tccagcccca
     7021 agtgtgatat acttacggtt ccccttattt tggctctcat ggaagtatcc tgccctaatg
     7081 gcaaactgtc ttgcgtacca gtactcaacc ccagtggcaa tgtatatttc ctgcatttcc
     7141 tccttgaaac cacctggggc gtcgtaaaac gattgaatca taccctgcac aaccgatacg
     7201 tttggatcca ttccgcttac aatctcatcc tggctgttgt aaacaggtgg agtaggaact
     7261 agcagcttgt ttaaatccag tagatacgaa aagccattgt attggtcgat gttaagcgat
     7321 aaccttccac caacccttag gttaatgggt ataaactcct tttgtggatc ctcattatac
     7381 gatagttttg ttccaatatt ggagatatta ccaccaattg ccatttcgcc acttttcccg
     7441 gcaaggttaa tagcgttctg gtagtatact gccaaatcgg ctgcaaacga ggtaccgggt
     7501 ttaccgctgc cagtgcctgt ggtagtaaaa ttaccggtta aatcggaacg gatgtaacgg
     7561 aaagccattg caccggcaat atggtcggaa aacttacgcg agtatgcaaa atcgattgca
     7621 aactcgttag ggttaaaggc ctggatgtag ttgttattgt tatccctgaa ggtgatttca
     7681 cccattgaga agtaaagcaa cgatccgcta atggattgca tcttatcaaa ctggtaataa
     7741 ccggttaaat gggtaaggtt gatatcgtta accaagtttc tgagccatgg ggtgtaggaa
     7801 aatcccatgc cccacttttt accttcaata aatggaaact tagcagggtt ccagtgttgc
     7861 gagtttacat cgggtaccga tgccactccg gcatcgccaa gggctgcact ccgtgaatcg
     7921 ggtgctatgg ttaggaatgg tacagcaatg cgcaatgcat ttaaaccacc cgatagcgat
     7981 atggtactat cgaactgagc cttaacagta ggctgtaatc ctactaaaag aattgttgca
     8041 attccaaaaa atttcttaat catcaactta acaattatga gtttgcaaaa ataacattta
     8101 tacagtaaca aatgtaaacg gtgtaatatg atacttattg tttaatatca aactaaagta
     8161 caacgaattt tgttgtggct acctgctcac cctgtaccga tgaaacttga atgcgagcaa
     8221 agtaaattcc ctttgcaagt ttctccccgt tggagttaca tccgttccat tcaagtggcc
     8281 ctattctgta accttcagta taaatccttt tttttaggag ttcggcaact ttagagccat
     8341 tgatgttgaa tatttccagc tttacatcaa gttcagtttc attggtgttt gtttcaaagt
     8401 agaactgtgt gttatcctta actgggtttg gaacacagta aaagttacca accacaagtt
     8461 tgtcgtcgcc aattacccgg aaaactattt tgccggtatt gctattatta aaggcatccc
     8521 aggcctttac ctctatagtg taagttccag gagttagacc agagaactga taggttgcta
     8581 ttccctgagt gtaatcgtcg gtattggctt tgtaaaaatt attaaggttg aatgtttcgc
     8641 tttgcccggc tggatatgtt aatgttgcgg taagatcatg ccctataccc gctccggttg
     8701 tgtttatgcc actcgcatcg gaaaggtaaa ccagtagcag agggttggag ttgcaaatac
     8761 caccgttagt gaacttggta tcgttcagga agattttaat ttgagggcct tcactatcaa
     8821 tgctttcagg gttaccaatt cccccaacaa ctattgaatc gtgtacgcct agggctgtct
     8881 gcatgccatc ggttgcaaaa agacttattt ttccattccc gtatgagaag ttaatatctt
     8941 ttggtaccgt aaaatcgata ctaaaagtac cctgtgaaac agttgctttc cctttaaata
     9001 tgatattatc gcgactttta aaggtcatcg gagtgccgcc gtcgttagcc agggtggtaa
     9061 tttcgcgttc cttatcgtat agggtaaccg aaacggttcc attgaaattt tcaacagttg
     9121 tgtcaaggtt atcggcaata aaccctttaa ggtttacctt attaagtgcc tttaaagtat
     9181 cagctggctc tgatgctggg cgttcgttaa tatatgagat ttttatgttg tatgtagggt
     9241 attttagcat caatgcggga tcgcctaata gggtgaagtt gcgtttattg taaccggttc
     9301 ccgatagtat tttggttatg cgaaccaaat caccaagcct gagatagttg ccattggcat
     9361 cggttttgaa aagctgctgt ataaagttgt agtttaaagt gaagtttggg cttgagtaaa
     9421 caagccgggt agtagagagt aacgctattg caccaccctt tggggtaagt agtatccatt
     9481 ccccggttga ggtcatgtgg taatcgtcaa aacggctaaa ctcgcaggtt gctgtaacaa
     9541 atagaggaag gcgtttagcg tttttccatc ccaacacatc gtttagcatt aaaaccttct
     9601 cgtgggcaag ccacctttca ttcccatgac cagtatagtt taaaagcaaa gcgccattgt
     9661 taacagcaga attgatggca ctggtaacat cggggtagcg ttgaccaccc gaaacaacct
     9721 cctgttggta agcatcgaaa aatatttttt ggatattata tgccgggtaa ttggtttcaa
     9781 tgtactttgc cagcgtgttg gcatcctgca tatgaacgtt accatcctcg tcgtcgccta
     9841 tgaaaaccaa tttgctttgc cagtcgtcaa tatcttcatt ctccatgtaa ctcttaattt
     9901 tggcaattaa attggcggct tgttccggtg aactaaccgg tagccgacca attccaatgt
     9961 ctacatagcc tgaggcttcg ccttcattat catcgagtaa tccaaagaaa tcgtccgata
    10021 caaatgactc aaccctgttt attgagcgag gcgactggta tgtgggaata aaattagtat
    10081 tgacctggct attggaaagg ttatcgaatg agccatcgcc gaataggagt aggtatttag
    10141 gtttatcgtc gtcgttagtt gcggctttat aaaagtaacg tagcatattg cgaatagctg
    10201 caacatcggg gttaccaccc gaaaactcat tgtaaacctg gtcggtagta taaacagcaa
    10261 ctgagaggtt gcttctttgt cggtggagtt gaactagctc ttcggcatag gttttaaaac
    10321 ttggatgtgt aactataaag taatcgaaga agccactccc gcgaatattc tggttatcga
    10381 catttgcata ccctacaaca ctgtaggctt tatcgggctc aaaggcaata aaggttttga
    10441 gagtattggt agcttgcttg aatgttattg tgccattggc gtaggaaata ttaacccttt
    10501 tggcctcatt gatatcagta atgtcccaaa cctgaacgga gctgtttgca ttgctgattg
    10561 tgaacctgct aattcttcct gcccctaccg attgaaaatc ctgaaagggc agttgggcat
    10621 tactatacga taagccatgt cggctctgaa tcgatataaa gtccaggaaa cccactgaac
    10681 ccgaattggg tttattgtag gtaaggtcaa atgttaggtt ttgtccaaca gtattacccg
    10741 agaatacttt ttcgtttaca gaaaccacat tggctagctc atcgccaatt attacggaat
    10801 ttaaagttat gctaccaagt gttccattat ttgtaagggt aaaagttgag gttgacgaag
    10861 atcgtgccgc tactcttacc catactttat aggtggagtt gtttactggg agtggtgttg
    10921 atacattgta gctgtaggtg gtttttatgt caaaaatttc gccaaaccat tccctgcccg
    10981 atttaacaag gtttgtatcg ttgctttcaa aggttgttac ttgatcgaac tcatttgttg
    11041 cataattttc ggtttcggtt ggcgtgtcag caaaaccgat gatgctttga ggttgactgg
    11101 ttgttaaaaa gtagtaaatg gttttggtgt atccatgctt ttcccatttc catgcactat
    11161 atgtttcatt atacgttaaa gtttcggggc cctgagcgta gaatagaata tagtcgccct
    11221 gattaaaaat gccgtcggca cctttactta tttgtattgg tattggagct acctcatcga
    11281 ggttgctttg ggcattcata aggggtaacg actttcctcc gattccccaa atgctaatat
    11341 tttctgggtt gctaaaacca tacctttgta aatcttcgaa tgaaatccta taaatcccac
    11401 ttttttgcac gcccactttt acccatttac ctgaagcaag aaccgagttc gaaagcgagg
    11461 tacgaatcgt tttatcaata tttttttcat gggaactgat gggctcaatt ctgaagctaa
    11521 acgattcaag tttctcaatt gagccagagg cattgttcct cattgcgggt attgaaaaaa
    11581 gcagaaccct gtttgcccca accctcaagc tttttgttgt tggatttggt tcagtttgaa
    11641 caatactaga atcaaaatct ctattgagtt taccccattt tatatgtgtt atttcaattc
    11701 tgtactcctt actatcggga attggtatta attcggtata gtatgggacc ttactggctg
    11761 ggtcaggaaa atcttcgcca ttaaaccata aacagttgat attttttttg tcggtttcaa
    11821 cccattcaag aattctgtgg aactcctgtg taaaagcccc caatgcttgt gttgttaata
    11881 atatgatgaa taaaactgcc ctcataattg actgatgtgt tgcaaagaaa caaaacttta
    11941 ctttaccttt acgtttgatt ctaacaataa aaaacgaata atgttagtta ttattacaaa
    12001 ccagttaaat gatatctaaa aacagttttt ttttattgta cttgctgggc ttagtcctat
    12061 gttttgaaaa atcacatggt caggatcctg tgttttcaca ggtttttttt aatccaacca
    12121 tgctcaaccc atcgtatgca ggcgcatcaa aggatatgcg attaggcatt atatatcgca
    12181 atcagtggac catgatagat atgccatact caacctttgg ggtaagtttt gacaggagga
    12241 ttgattttgg cccaagctgg aaacactcgt ttcgtaacaa tcgttctcaa tcctcagggc
    12301 ggagcggctt tggtataaac attatgagcg atgtggaagg gaaaggaaca ttctcccgga
    12361 acactctcga tcttatttac tcatacggaa ttcaacctac atataactca cacgtacgat
    12421 ttggacttca aacctcagct atttttcgta cacggagttt ttcaggtttg gttttcccgg
    12481 atatgattga tccggctggt aatgtgattg gaaattccga cgccgtaggc tatacaacct
    12541 ggaattacga tatagggctt ggtgttgccg gtgattttga aaatttttat ggtggatttg
    12601 cggtgcatca tcttttacaa cctgttgagg ccagtttctc gggcggaaaa gcatacattc
    12661 cccgtaagta tactttccat attggtgccg attttaacct atataagtgg agaaggttta
    12721 aggaggttct gcttttttct ccaaacgtaa tctatattca acagctaaat tttaatcagc
    12781 taaaccttgg cttttacctt tcaaagaact gggcagttgc aggactctgg ttcagggaaa
    12841 atttaagttt aaatagccat tcgtttgttg ttaccttggg atatgccgat tatatgtttc
    12901 gtattggtta cagttacgat ttcagtatta tgcaacatgg tcttagaggg ttaccaacct
    12961 catcgcacga ggtaacctta gggtggaatt ttgagtataa aagggggaaa aagaaatacc
    13021 ggtacatcaa atgtccaaaa ttttaatttt tttttaaact tttagggcgc attacaaaat
    13081 attatttata tatttgagtt taattataat gagaaaatcc gtacagctat gagcttaaag
    13141 tatagaatct tattattggc atcagccgga atttttggtc ttaactcttg cggtttattt
    13201 ggtggtggtg gtggcggaac tggagcgtcc gaaaaaactg gctgggagta taacagccct
    13261 gataatgggg gctttgaggt agttgaaaac tggcgacccg aggcaggtcc cggcttggtg
    13321 ttcattgagg gtggtacatt tactatggga cgagtagagc aggatgttat gtacgactgg
    13381 aatgctaaac cccgcagggt aacagttaca tcattctaca tggacgaaac cgaaattagc
    13441 aatgtagatt ggcgtgaata tgttcactgg attaaacgtg tatattcaac ctatccacag
    13501 gtaattaaaa atgccctacc cgatactctt gtatggcgtt cgccccttgg ttacaatgaa
    13561 ccttttgtaa ccgactactt caggcatcct gcctataacg attatcctgt agtaggagtt
    13621 aactggcttc aggcaaacga ttactgtata tggcgtaccg atagggtaaa tgagcgtctt
    13681 ttagttgata agggcattat tgaatttgac ctggctcaaa aaggtgctga tgcatttagc
    13741 actgaggctt acctttcggg ccagtacgat gcgcgcgtta agaagctttt acccagcctt
    13801 gaccctgaaa aacccgaagg acgtcacgtt cgctttgaag atggtatcct gctacccaag
    13861 tatcgcctcc caaccgaggc tgaatgggag tttgccgcat cgggacttgt aggtaatacc
    13921 tatgacgagc gcatggttga gcgtcgtatt tacccttgga atgggcacaa cgtgcgtaat
    13981 cctgagtcaa aagttcgtgg ccagatgatg gctaactttg ttcgcggtcg tggtgactat
    14041 atggggcttg caggtaattt gaacgacgag aacacattcc ctggaccagt taaatcatac
    14101 tggcctaatg attacggcct atactgtatg gctggtaatg ttaatgaatg ggtgcttgac
    14161 gtttaccgcc ccttgagttt tgaagatgtt gatgaaattc gtccgttccg tggtaatgtt
    14221 ttcaaaaccc catatgattt agacggtaac tactccaaga aggatagttt aggtcgcata
    14281 ccctaccgtg aagttaccga ggaagaagca gctaaccgac agaactacaa taaagcctac
    14341 tatatcaact ataaggatgg tgatgttgcc tcaacgcttg atttcagaga tggacaaact
    14401 gtgaaagaga aaaactccga caggatgtac tatcagggcg atactcaaca gaagaatgtg
    14461 cgtatgacaa ctctgattaa cgaccatgtt agggtttata agggtggttc ttggaaggat
    14521 agggcatact ggttaagccc aggtacccgc cgtttcctcg acgaagcatc atcaaccaat
    14581 gatattgggt tccgttgcgc aatggaagcc gttggtgctc aatcggcaaa gaaacgtggt
    14641 gagagaattg gaggaaaata gtcaatataa atcaatattt ttggaaaacc tcatatcttt
    14701 atgaggtttt cttttttact ggtttgttat gattgaacat atttataaag aatttgaaaa
    14761 atcctatcac gtaactatcg atagccgaaa ggttaaagct ggctcaatat tttttgcttt
    14821 aaagggtgaa aataacgatg ggaatgccta tgctgccaat gctttgcagg atggagcagc
    14881 ccttgctgta attgataatc cccattacat gaccaagggg tgtgtgttgg tggagaatgc
    14941 cttaaatgca cttcagcaac ttgcaaatta tcataggcaa aaaatcaatg cacaaatttt
    15001 tgctataaca ggttccaatg gaaaaaccac taccaaagaa ttattgtaca ctgtcctttc
    15061 tcaaaaattt aaaacttatg caacggttgg taatttgaat aaccacattg gggttccctt
    15121 aacgttacta tccattactc cagatgttga ttttgcgatt atcgaaatgg gtgcaaatca
    15181 tccaggtgat attagcgaac tttgcagaat tgctgaacct gatttcggtt tgattaccaa
    15241 tattgggaaa gctcaccttg gtggttttgg cggttttgaa ggtgtaaaaa atacaaaggg
    15301 ggaactttat aactttttag ccttgagaga tagggcaata ttttacaacc atcaaaatcc
    15361 tattcttgta gaacttttaa ataagtataa agcaaataac gttattccat actctaagta
    15421 ttttgaggat ttcatagttg tggagaagcc cgaaagccct ttcctttcta tttcaataag
    15481 tgagggaggt aaactcacaa ctattctgac taaccttgta ggccaatata atgtggagaa
    15541 tgttatggca gcttatgctg ttggaagaca ttttggagtt ggccctggta ccataaaaga
    15601 tgcgattgaa agctatattc cctcaaataa tcgatcacaa cttttaaaaa cccagaacaa
    15661 tactgttatt atggatgcat ataacgccaa tccaactagt atggaaaatg ccttaaaaaa
    15721 tttggcaaaa ttacatgcat caaaaaaggt tgctattcta ggtgaaatgc tagagttagg
    15781 ggaatatagt ctcgatgagc ataaaaaaat tgttgagctt gccagcgctt tgaattttga
    15841 taaaatctta cttgtgggta agggcttttc aagttttaac ggtatgtttt attattttga
    15901 taattcttct gaatgtgctg agtatctgaa gcagaatcca attaaggact ccatggtgtt
    15961 ggtaaagggc tcccgtggtg taaaacttga aatagtggtt gaggctcttt aatttttaaa
    16021 aaagcaaaaa tcctaattaa taaattcatt cttttacggc tcttccaggc cttaatctgg
    16081 cgttcgcgct tacgtgcctg ctgtannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16201 nnnnngcctt cgataaagtc gaaggctttg tgggagctac agggatcgaa cctgtgaccc
    16261 tcccgccgga tgcgggatgc tctgaaccgt agggttcagg gcttactgaa cccacacagt
    16321 tcctcaaccc ttttacggct cttccaggcc ttaatctggc gttcgcgctt acgtgcctgc
    16381 tgtatggagt cgaactgctc gaggtaaacc agcctccaat cgttcgccat ggaggtgaac
    16441 cccctgtggg gtgcgttgtg gcgccggatg cgttgggaaa tctcatcgga ggtagacccg
    16501 atgtagaaac ggtcggtaga actggagtaa atgatgtatg tgtagtgcat acaaaaaaag
    16561 ccttcgataa agtcgaaggc tttgtgggag ctacagggat cgaacctgtg accccctgct
    16621 tgtaaggcag gtgctctgaa ccagctgagc taagctccca atattttatc tcagcctgtg
    16681 accctcccgc gaggcggaat gctctgaacc agctgagcta aactcccaat attttatctc
    16741 agcctgtgac cctcccgcga ggcgggatgc tctgaaccag ctgagctaag ctcccaatat
    16801 tttatctcag cctgtgaccc tcccgcgagg cgggatgctc tgaaccagct gagctaagct
    16861 cccaatattt tatctcagcc tgtgaccctc ccgcgaggcg ggatgctctg aaccagctga
    16921 gctaagctcc caatatttta tctcagcctg tgaccctccc gcgaggcggg atgctctgaa
    16981 ccagctgagc taaactccca atatttggtc tttttttatt ggtcattccc cgttggaaat
    17041 gcgatgcaaa agtatgcgaa agtttgaaat ctgcaaaata ttttcggaaa aaattaaaga
    17101 aaaaattttt aaaatcttta attctattga taatcagcaa tcaaaaacta tacaacctca
    17161 gcaactacaa aagtgcttcc acccacaaaa attaaatcgt ttggattgga ggcttccttt
    17221 gctgccgcta atgccaaagc gactgttgaa tatgagtgac cgtatagttg gtgttctgcc
    17281 gccctctttt ttaattcttc gtggtttaat gcccttggta ttgcagcttg ggtaaagtaa
    17341 taggttgcat ttttaggcag tagtgccaac ataccatcaa tgttcttatc ggcaactacg
    17401 ccaattacca tgtgcaagtt attgtgagga atgtgcctga gttgttccac aacgtattta
    17461 ataccatcta cattgtgtcc tgtatcgcaa tagatgagtg gattctccga taatttttgc
    17521 cagcgtccca taagcccggt aagcgtttga acattcctta aaccttccat tagcgcttcg
    17581 ttggagagta aaaacccttt accgcttagt atttctattg attttagaac cccagaaata
    17641 ttgtaacgtt ggtaattgcc taacaggtca atctcaattt taatatctct aaccttatca
    17701 gctagtttta atacctgtaa ccctgtagta tctatatact gttcgtaaac ctgaatttct
    17761 tgatcagcaa aatagatagg agcattcatt tcctttgcct ttgctataaa gacgttttca
    17821 acctcaggtt gatacttact tacaacaact ggtgtatctg gtttaataat tccagctttc
    17881 tctgatgcaa tttttggtaa tgtatcgcca agcaagtcgg tgtgatcaaa cccaatattt
    17941 gtaattacac ttagaatggg gttgataata tttgttgaat cgagtctacc tcctaaaccc
    18001 acctcaataa ctgcaacatc aaggttcatt cgggagaagt aatcaaatgc cagcgcaact
    18061 gtcatttcaa aaaatgacgg cataatttga tcaaaaaaat ccttatgctg tgaaatgaac
    18121 ttagcaacct catcttcggg aatcatttca ccatttaccc ttattcgttc tctgaaatcc
    18181 cttaggtgtg gggaggtgta taacccaact ctgtatccag ctttttgaag aacggcagcc
    18241 agcatgtgcg atgtgcttcc ctttccattt gtccctgcaa tatgaatggt tttaaaggtg
    18301 cgatgtggat gcccaaaata ctcatcaagt ttaagggtat tccctaggtc agctttgtat
    18361 gctgctttcc caaccctttg aaaaataggt agatgtgagt agatgtaatc aagtgcctcg
    18421 ttgtacgtca ttttacttgg ttttgggtgc aagtttagtg attttctcgc tcaaaactta
    18481 ttagctgtcg gttaacaaat atttagggtg ataatgattg ttttgattga gcttttctta
    18541 tatttagatg aatttttaaa tcgcaaaact atggcaaaga gtaaagtgaa taagattttc
    18601 gtgattgata ctaatgtcat tcttcacgac tttcagtgtt tgtataaatt tcaggacaat
    18661 gatattgtta ttccaatagt agtacttgag gaactagaca agtttaagaa ggggaacgaa
    18721 atgattaatt accatgcacg ggagtttgtt agggagttag ataaaattgc cggcgataag
    18781 ctttttaacg ggggcttgcc cttagggaaa aatctaggaa aactcagaat tgaaactggt
    18841 aagccaatgc cccctgaaat gaaagaatca tttgctgagt tcacgcctga taataggatt
    18901 ttagccataa ccattcattt ggttaagcat aatcctgaac gtccggttat acttgttact
    18961 aaggatatca accttagaat gaaggccaaa tcgttgggca ttgttgcaca ggattacctt
    19021 aacgataagg ttgaagatat tgatagtctg aagaaagaga ttgagataat tgaaaaggtt
    19081 gatgatgcac taatccaaaa gctttacaat tccgaggaga aacttttagc aaaggattta
    19141 aagttaaagc cttacgctaa tcagtactat attttaaaag ggaacaaatc gagtgcactt
    19201 gctcattacg ataaggcaac cgataccctt gatcgtgttg aaaaggttag agcatatggc
    19261 attgatccaa gaaatgcgga gcaaacgttt gccctggatg cactcttgag gcctgatatt
    19321 cagctagttg cgcttaccgg aaaagcagga acaggcaaaa ccctacttgc tctagcggca
    19381 gcattacaac aggaagagca gttcgatcaa attctacttg ccagaccaat tgttgcgctt
    19441 tccaataaag atattggctt tttaccgggt gatgttgatg aaaaaatagg gccatacatg
    19501 cagcccctat tcgacaacct aggtgttata aaaaataggt ttaaaccaac aagcaaagat
    19561 tacatgcata ttgaagagat gcaacgtgcc gataagcttg taattacacc attggcatat
    19621 attaggggac gaagtctttc aaatgttttt ttcattgttg acgaggctca aaacctcact
    19681 ccacacgaga ttaagacaat tataacccgg gcaggtgagg gtactaagtt tgtatttaca
    19741 ggcgatatac atcaaattga ccacccttac ctcgatatga aatcgaatgg gttaacctat
    19801 ttaagcgata gaatgcatgg ccaggagata tttgcccata ttaaccttgt taagggcgaa
    19861 cgtagctacc tggctgagct ggctagcgat ttgctttaac ttaaaccaga ttataaatgg
    19921 caaaaacgtt gctcatactc cgtcatgcca aatcctcttg ggccgaggcc gataaagcag
    19981 ataaggatag ggcattaaaa gccaaaggta taagcgacat tgttaacacc gcaagggcta
    20041 ttgcacccaa ggtgcaaggg ttagatctga ttattactag ttctgctaac cgtgcggtgc
    20101 atacagcaat actttttgcc gaaaccattg gttttccgca tgataaaatc cggatagagc
    20161 caatggttta ccaggccgat gaaagagagc tggtggagat gattaaatca ttacccgatg
    20221 aatacaaaag tgtaatggtg gtagggcata atccaacact tacctatttg gtaaacactt
    20281 ttgtagccaa tcgcattcat gaactaccaa catcgggact tgttggggtt aagtttgaca
    20341 ttaacagttg gatagaactc tctgccgaaa aggtatattc tgtttttcag agctttgatt
    20401 ggtagtttaa caatttctta acatcagtgt ttaacctgta aacggttaat caattaatat
    20461 tgcccgaaat actagatttg aatggcacca aaaaaaatac ctcttgttaa ccgtgagcta
    20521 agctggttga catttaatgg cagagtgttg caggaagccg caaatccatc agtaccgtta
    20581 attgaacgta ttcgtttcct tggaattttt tcgaacaacc tcgatgagtt tttccgggtg
    20641 agggttgcca ccttacgccg tttattggtg gttgaaaagg gggcaaaaaa gattatgggc
    20701 gataatcccc gtaaaattct cgaaaagatc caacggatag tcattgacta tcagaaaaag
    20761 ttcgacaaca catttcagga aattattgat gagctcagaa aggagaatat ctatctcatt
    20821 aacgaaacac agctatcagc tgggcaaatt gattacctta acggtttttt cgatgatcac
    20881 atttcggaat ttgtggcacc ggtaatttta aagcggaaca ataaattccc tgaactggtc
    20941 gatcaatcca tttacctagc ggttaagctt acgaacagtc aaaaacccaa tacaaaggag
    21001 tttgcactca tcgaaatccc ttcgaggttt gtaaagcgtt ttatacagtt accgaacgaa
    21061 ggcgataaaa gttttattat cctcctcgac gatgtaatca ggctttgcct atccaaggta
    21121 ttcaagatac ttcctttcga tacttttgag gcatacactg taaagattac gcgcgacgca
    21181 gagctcgatg tggataatga tatttctgag agccttttgg agaagatatc aaagggagtt
    21241 aagaatagga aacgtggcga accggtaagg cttgtttacg atgaaaaaat tcctactgac
    21301 ttgctaaagt tcattgtgaa agggcttaat ttggacgagg atgattatgt tattccggga
    21361 gggcgatatc acaacttcaa ggattttatc cgattcccca gcttgggacg aaatcacctt
    21421 ataaatttac cgcttgagcc cgttagtgtc aaggaattag atgaggctac tcgcataatg
    21481 gatattatgt ccgatcacga tttcatgctt cattatcctt tccatagctt ccagtattac
    21541 attcgcttat tacgtgaggc tgcccttgat cctgctgtta aatcaattca tattaccctt
    21601 tacagggttg ccactgaatc gcgtgttgtg catgcattaa tcaatgctgc tcaaaacggt
    21661 aaaaaagtta cagccatagt tgagttgcgc gctcgtttcg atgagaatgc taatatatat
    21721 tggtctaagc aaatgcaaga ggctggcgtt caagtgatat tcggggtgcc tggtttaaag
    21781 gtgcatagta aaatgacact tattacccgt caggaaaaaa acaaggaacg taagtatgcg
    21841 gtggtgagca ccggtaattt tcatgagggg aacgcaaaca tctatacaga tgttaacctt
    21901 tttactgccg attcaagaat aacctctgaa atacttaaag tttttacttt tcttgaaaca
    21961 aattacaaga cattttcata caagcatttg ctagttgctc cattcaacat gcgcaggcgc
    22021 ttatactcat taattgatac tgaaattgag aatgctgcca aaggactccc tgcctacctt
    22081 attggaaaaa taaataatct ggttgacgag gagatgataa agaaactata ccaggcgagc
    22141 aaggcaggtg taaagattaa acttgtggta aggggaacat gtgcacttgt gccaggcgta
    22201 aagggcatta gcgagaatat tgaggtatat ggaattgttg ataggttttt ggagcactct
    22261 cgcattttca tatttgctaa tgggggtaat gaagtatgct atatttcatc ggccgactgg
    22321 atgacccgta accttgacta ccgcattgaa gtggcaactc ctatttacag taaaccccta
    22381 attgaggaga tgaaaacggt ggttaactat gcgcttcgcg acaatgtgaa agcgcgtttg
    22441 gttaaccatg gtggttgcaa tgagtttaag ccctgtaaag agggtgaacc cgagtttcgt
    22501 tcacaactgg aactttatag cttttatcaa aatattgaaa ggcacaagaa cggagaggta
    22561 aaatgaagat attaaagttt gccgcaattg acataggttc aaacgcagcc cgattgctac
    22621 ttacaaacgt ggtggaggat ggagaaactg tagtttttag caagtcctcg ctggtgcgag
    22681 ttcctatcag gttaggcgag gatgcttttt ctattggtta tatcagtgaa cacagagctc
    22741 agaaactgct caaaaccatg attgctttca aaaacctgat ggaagcgcag gatgtggttg
    22801 cctatagggc atgtgccaca tcggcaatgc gttcgagcag caatgcgcct gaacttgtta
    22861 gtatggttaa acaacatgca aacattaaca tagagattat tgatggatca cgcgaagccg
    22921 aaataattta cgctaatggt attgcagaga tgcttgacca tgatagacct tatatatatg
    22981 ttgatgtggg tggtggtagc actgaaatta ctgtttttga gaaaggtgaa atcaaggcat
    23041 caaactcttt taatattgga actattcgca ttcttaaggg aatgattgga aagcccgatt
    23101 ttgaccaggt acgcgactgg attaaaagca tagtaaaaaa gaagaatcaa cctaaaatta
    23161 ttgggtcagg tggaaatatc aataagcttt acaagcttgc tcgtaaacca ataggggagc
    23221 ctttatatca caaggaagtg aaaaatctat acgatttttt ggacagttac tctgttgacg
    23281 ataggattaa gttgcttggc ttaaaccctg accgtgcaga tgttattata ccggcaacca
    23341 aaatctttct caaggtgatg aaatggactg gtgcaaaaga ggtgatagtt cctacaatag
    23401 ggatttccga cggtattatt cgtacgcttt acgctgatta taagcaaagc cttgcgttgc
    23461 agcaatagca ccatctgtta cattctaata ttcatttggt gtaaacctta atggttttca
    23521 tttgttacct ttgcaaaaat tttttacaat gaaccagggg agaaagcgtg ttgcgttcta
    23581 tacgctgggg tgcaagttga atttttcaga atcatctacc attgcccgcc agtttgccga
    23641 tatgggctac gaacgtgttt cgcccgattc ggaggccgac gtttatgtaa ttaatacctg
    23701 ttcagtaacc gagcatgccg ataagaaatg ccgtcaggca atccgaaagt ttacaaccaa
    23761 atcgcccaac gcaatggttg ctgtggtggg ttgctacgct cagctaaagc ccgaagaaat
    23821 tgcacaaata gatggggttg actttgtgct tggagcagct gataagttca accttcctca
    23881 tctgattggg aatgatgcta aaaagacaaa agtaaaagtt tatagctgtg atatcgattc
    23941 ggtaagtgat tttttcccgg catactcaac tggcgataga acacgatcgt tcctcaaggt
    24001 tcaggatggt tgcgattacc attgcagcta ctgcacaatt ccccttgcgc gaggtaaaag
    24061 ccgcaatatg cccattagtc aaatagtgga tcaggccaaa gctatagcca gtcagaatgt
    24121 taaagagatt attcttacag gtgtcaatac aggcgatttt ggcaagtcct ctggcgaaac
    24181 attcctggaa ctcatccaac agctcgatag ggtagaggga attgagcgct tccgaatatc
    24241 gtccattgag ccaaatctta ttacccctga aatggtcgat tttgtggcac aatcggctaa
    24301 gttccttccc catttccata taccgcttca atcgggttca aataggatac ttgcgcttat
    24361 gcgcaggcgt taccgccgtg aactttttgc cgaacgcatt gagctaatcc gaagccgtat
    24421 gcccgacacc ttttttggtg tagatgtaat tgttggcttt ccaggcgaaa ccgacgacga
    24481 ttttagcgat gcat
//
LOCUS       DAOSAL010000034        24297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_1496_length_24297_cov_52.506353, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000034 DAOSAL010000000
VERSION     DAOSAL010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 24297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24297
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1496_length_24297_cov_52.506353"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(869..2254)
                     /gene="gatD"
                     /locus_tag="PK049_06325"
     CDS             complement(869..2254)
                     /gene="gatD"
                     /locus_tag="PK049_06325"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF003217.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatD"
                     /protein_id="HOK61209.1"
                     /translation="MEDIFQGYKGRALEVLKKFNVRVWGKAIIETTRGEFQGTVLPRA
                     ENDDDTHIVLKIATGYNIGIDIFTIKAMKEVGYYKATYKIPEKEFPYTPNLPKVKLLG
                     TGGTIASRLDYRTGAVIPAFSPGELYGAVPELADICNLSTEKLFAVFSENMGPEQYKA
                     LAIAIGKEIENGIDGIIIGHGTDTLSHTAAALTFMVQNSPIPIVLVGSQRSSDRPSSD
                     AALNLMHAAYTAGHSDIAEVMVCMFGPTSDEYGFLHRGTRVRKMHSSYRSTFRTIGDT
                     PLATVSLKDGVKPIKDTYNHRRKDRKAKILPYFEEKVSIVYYYPNMKPDIIDSLVDNG
                     YRGIIIAGTGLGHVNKPLYPAIERAKKKGVHMFMTVQTLWGYAHMYVYDTGRDLMAKG
                     IIPAENMLPETAYIKLGWVLGQTDDPEEVKRLMLTPINDEITPREPYNGYLIYQGGVP
                     EVEEFIRTFRK"
     gene            complement(2280..4277)
                     /gene="gatE"
                     /locus_tag="PK049_06330"
     CDS             complement(2280..4277)
                     /gene="gatE"
                     /locus_tag="PK049_06330"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF003107.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatE"
                     /protein_id="HOK61210.1"
                     /translation="MAKKQTPGKHFDPQKNYRETMKAIGYVTRAEATPKDYERIGFKS
                     GLEVHQQLKTKQKLFCRCPAGIYHDDNDYHAEIIRHMRPTLSEMGEYDGTALMEFKTR
                     KEIVYRINNKTTCTYEIDDTPPFPINREALEIALEISLLCKLNIVGEVHITRKQYLDG
                     SIPTGFQRTAILGVEGQIQLKNKKVGLIQLSIEEDACREISDVGHRRIYKTDRLGMPL
                     IETVTHPQCLTPDELREAADYIRFLNRSTGKVRTGIGAARQDTNVSCEGGTRVEIKGV
                     SHNKWLPMLSHNECFRQWALLHIRKLLLERVPLPEKWVMRHIEIDPYSQGIEHAVANR
                     FADMGYKVVAIALPAFHSILSHFTQPGKCFTNELADRLKVIACIERPNITSNEDLEIP
                     LSESDTTRLASLLKASPDDAIVLIWGPANDIPTAIETIEERCRMAFEGVPRETRRPLA
                     DGTTIFERVLPGADRMYPDTDSAPIPLDEAYIEKLRERLPIDIWHRFQQLAQMQIPED
                     AHPFLLRRNLVPVIEEIVGLGFPSRYVGSLLGHKLKRIEGKYPKHKDFNYSRIVELFR
                     FIKENKLHHSIAKLMLPVVYQYPNMDFNSVLTSINFKRKTEEELLAPVDYLIEKFKEI
                     KIAKKDKPENAINWVMGQLHKQALGNVDPAVLHSRIKQRIS"
     gene            complement(4738..7992)
                     /locus_tag="PK049_06335"
     CDS             complement(4738..7992)
                     /locus_tag="PK049_06335"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOK61211.1"
                     /translation="MKFTKIILALALASISISGSSQDQVEWYRYPAISPNGKTIAFVY
                     KGDIYTVSASGGEAVALTANPAHDYLPVWSHNGDYIAFASNRYGNMDVFIVPSRGGEP
                     KRLTYHSADELPYAFTADDKHVLFGAARYDMASSRLYPTASQPELYSVPVNGGLVNQV
                     LPVPAEWVSVSGDGKLLAFHDKKGGENEFRKHHLSSIARDIWIYNTQTQEYNQLTNYK
                     GEDRNPVFAPGDSVIYFIREDGNFNVFKLSLSNSSEVKQVTNFTTHPVRFLSVSQNGT
                     LCFGYDGSIYTMGNNGISNKVDIRINRDFQQNPEQTVSLSGNIRELSVSPDGKEVAFI
                     ARGDVFVTSVEGAFTKRITQTPEQERFVAFTPDGKSLVYASEREGRWQIFKASRVRPE
                     EPFFFASTLINEDLLIGNEYDNYQPLPSPDGKKLAYIEDRTTLKVLDLESKSAKTLLT
                     SKELYYMSDGDQRFCWSPDSRWLLVEYSPTMSNNEIIALPADGSEPFRNLTQNGYDDF
                     TPRWSNGGKQVLWLSTRDGLRSYANSGMRQSDVYTLFLTRDSWDKFRLSKDEYKLLKA
                     IEDKQKETKQKKDDDEKGKKGKDKKDNEKVDSSLKFDWEGIEYRTARLTTSSADIADA
                     LLSKDGETLYYLARYDKGYNLWSMNIREKEAKILVEMDVDYAKLYWDNDEKNMFMLAD
                     GKIYKVDLDKGKREALKIAAELTVNTDAERQAMYEHVVIRTRKGFYTPTYHGIDWNFM
                     AEHYRKFLPHIGNGFEFAELLSELLGELNVSHSGARFYLNNPNGDQTASLGIIPDVTY
                     QGQGIRVDEILLGGPLDRKELSLSTPFIITAIDGNTIGTDKDWAYYLNRKADRYILLT
                     LTELSNGKERQVTVKPTNLSQESRLLYRRWVKRNADEVEKLSNGTLGYVHIPGMSDGP
                     YRNIYGEMMGKYYDRSGVVVDTRFNGGGDLVSDLAMFFTGTKFITYHNHVRELGSEPT
                     FRWTKPTVALVNEANYSDGSCFACGYKQLGIGKLIGMPVPGTCSFAGWETLQDNQIRW
                     GMVPVSAKDMYGVWMENVEAIPDYVIMNEPSVAPKGRDQQLEKAIKALMEATVK"
     gene            8113..9261
                     /locus_tag="PK049_06340"
     CDS             8113..9261
                     /locus_tag="PK049_06340"
                     /inference="COORDINATES: protein motif:HMM:NF013761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HOK61212.1"
                     /translation="MFSLSNTEHVFEGRSNASLRCESLILSILSKKMVAKSLILLSIF
                     AKRLKIFRDFPGKLLVKRFFSHNSIDELKEISTQNFHNRILSCILPVSNGNGSTVNLL
                     SDLANDKYNIPGLEFLFLNVDDYLEPRILSLVSNDGFLNQVDKKAFQSFTDYVDTLCE
                     IASRNNKSIILYTANFAGFSAIHAIAIDLMQRYNTQKVTVFLLFHLCHLNSQNRIVDL
                     VRRGSSQGFTPGIAIAKYLTTEYVNKKFVDNSHNDSSCHGFNQTKRVFYEVTKFLLNN
                     IDHVSALLISHNPDNILYLTSLMEGCGVGHSNKNVTFLQRYGMAKSLTLNLANRGYNV
                     ATIIPYGNLTDTLRWMHGLYGFDPSLPNIFTEQRFAILRELNRRKLEN"
     gene            9268..9747
                     /locus_tag="PK049_06345"
     CDS             9268..9747
                     /locus_tag="PK049_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61213.1"
                     /translation="MKFDNQDTVYGIHSLKLVLAVVVVIVIVLMYLTSIPDYIESSIG
                     ISQGWVIAIVVILYLMFFGYFIWMDAAFISYNDEGNKLVIRTFKLRPWGGKKISMEIP
                     HSEFYKYEITHKWPKKQLHIYVKKGNKIMKYPPVSVVSLTAEQYQKMESSLNQLSKA"
     gene            9751..10872
                     /gene="dprA"
                     /locus_tag="PK049_06350"
     CDS             9751..10872
                     /gene="dprA"
                     /locus_tag="PK049_06350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOK61214.1"
                     /translation="MGESELQYRIAIELIPKVGSITAKRLIAYCGSAEAVFRQSKKAL
                     LKIPGVGETIAAEVVNQNVLHLAEKEIEFIERYKIKALYFTDPEYPERLRQCEDGPVM
                     LYVKTHSSINFNSDKIISIVGTRKATAYGKQTCEAIVNGLAAKGHNPIIVSGLAYGID
                     IVAHKSALKNNLQTVAVLGHGLDTIYPVAHRAVAKEMVEQGALVTDFISGTPFDPNNF
                     LRRNRIIAGISDATIVVESALEGGALVTADIANSYNRDVFAVPGNVTSTYSMGCNMLI
                     KQNKAALVESADDIEFLLGWSTTSKTQTVKQYTINFDHFTNEEKKIINFLKEKGEETV
                     DLIALGTGLPVSTVLSSLLNLEFSGVVSSKPGKVFALKN"
     gene            10877..12226
                     /locus_tag="PK049_06355"
     CDS             10877..12226
                     /locus_tag="PK049_06355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOK61215.1"
                     /translation="MSKQPKPNRIMELYKKVQRFLLHGIWSMNLDELPPKLKWPFKYL
                     RVLLLALKGFFEDKVIVKASALTYYTLMSIVPIFAMAFGIAKGFGFERYLEQQLTTQF
                     KGQEEVINKVIVFANSLLARTGGGLIAGIGVVLLFWSVINVLSSIEHAFNDIWQVEKP
                     RSWVRKFTDYLSIMLISPILLIASGSIHIFLATTVTSIAEKHEHVSYISPFLIDMLQF
                     LPYLLIWILFSFIFIAIPNTKVSYKSGIVAGIIAGSGFVILQWLYITLQIGVSRYNAI
                     YGSFAALPLFLFWVQISWQIVLFGAEISFAYQNVDMYEFERETTHISHKNRKLLALLV
                     LSTIVKRFMNGEKPATNLELSVNLKIPQRLMRNLLDLMVNCELLNEVVITESKDIGYQ
                     PALHVEHLTIAFVEEKLDSYGFEIEPDIPELKQVKQVCTSFTERYRELTASTLIGSI"
     gene            complement(12228..12746)
                     /locus_tag="PK049_06360"
     CDS             complement(12228..12746)
                     /locus_tag="PK049_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018451909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOK61216.1"
                     /translation="MNDTLRIILNRKSVRSFTGQEVSNELLEELARAGMAAPSARNLQ
                     PWAFVIVTNRATLDTLAERLPYAKMLFEAPAAIAVCGDLSKAGDSPEGYWVQDCSAAT
                     QNILLAAEAMGLGAVWTGVYPRPDRVAIVKEVLGLPDNVLPLNVIPIGFPKGEHQPKE
                     KFKVENIHWQNW"
     gene            13038..14987
                     /locus_tag="PK049_06365"
     CDS             13038..14987
                     /locus_tag="PK049_06365"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOK61217.1"
                     /translation="MTYSNSKKSISGIVAIATIVVVALVSSWMALSALMGNALAYQLN
                     LGLAIGSVPIKIDGLSAWFLLIINFTSITGAIYGLGYLKGYSNPAAKLNLHWIMFAIF
                     HLSMILVCIVQNGFVFLVIWEIMSLSSAMLVMFDGHNPKTLKAGINYLVQMHISIVFL
                     TVGFIWLYAVTGSFNFDSLQSFFIGNNNIWLFLVFFTGFGLKAGFIPLHSWLPHAHPS
                     APSHVSGVMSGVIVKMGIYGIIRVASYLTNNHLLLGEIVISISVLTGIYGILNAAAHR
                     DFKRMLAYCTIENIGIIGIGIGIGLIGISIKSDIIYFLGFGGALLHVLNHSLFKSLLF
                     YSAGSVYQQTHTRDMDKLGGLSKYMPKTAFLFLIGAVAIAGLPPFNGFVSEFIIYSGL
                     VNGFGTGDNGLTILLILSFAGLSIIGGLSLLTFAKTYGVIFTGTPRTSLDHKPHEVSN
                     LMIVPQLAIIAAMLSVAFFPSFYVNTINFIIKDFSNGLPINPAFSSDFSNTLKSIMVF
                     SLAVFAVILVVWGIRKISTKNKSILADETWGCGYTNPTPRIQYTGKSFSKQLGKIFNF
                     FLIEKKKYNELNSGEIFPHKRQYFSFYTDFFETRFINLITQRILFTANYFRFIQNGRI
                     QSYVLYGIVFILAIFILSALNLFSF"
     gene            14997..17024
                     /locus_tag="PK049_06370"
     CDS             14997..17024
                     /locus_tag="PK049_06370"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOK61218.1"
                     /translation="MEALKLFLTIPLIAFLAMPFMSIKWRGIVAYLAVILNAILSSYI
                     AINVLGGENYQFIFSGNAVTGEIPVRIDALSAWFILIINFTILTGGLYGIYYMREYLT
                     MRNSITLHVTAFILLHTALLSLCGLQNSIVFLLTWEIMALSAFVLVIFEHNKVSTIKA
                     GINFLIQSHVSIIFLLLGFVWVAAKTGSYDFLSISQFTHEHGNSVSLVLFIFLFIGFA
                     IKAGFVPFHTWLPYAHPAAPAHVSGIMSGVLIKIGIYGILRVILLQNTNLEVIGYIII
                     AFSAISGIYGVMLAILQHNLKRLLAYHSIENIGIIGLGIGIGCVGLGTQNLIVASLGF
                     AGALLHTLNHSLFKSQLFFSAGIVYQATHTVQIEKLGGLIKKMPQTAFLFLIAALAIS
                     GLPPFNGFISEFLIYSGLYNWLPSADSGIIITVIFSILALVLIGGLALLCFTKAFGIV
                     FLGNPRHQFKHQISEASFGQILPLYFITALIVAIGLFPNAFLTIVSKPLNLFIALDQN
                     NTSTSIGGNSEILQMISFSFALLLVFTAIIFGIRQMVQRKHAISADETWGCGYTGETK
                     KMQYTANSFARSYYKLFRFILLVFKKEKTVENVFPRHAEFESSPYDKIEKLLIDKPIQ
                     SIRFFMGKFTFLQNGRLQYYIIYGITFILAIIAIPLLYEKLLQLIEILKHL"
     gene            17027..17932
                     /locus_tag="PK049_06375"
     CDS             17027..17932
                     /locus_tag="PK049_06375"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOK61219.1"
                     /translation="MLSAILIILAAIFYTGIINRTKSIASGRKGPGLFQHLSDVIRLF
                     KKGAVYSKTTSFIFQIAPTIYFSTVVIAMLLVPIGNSKGLFSFQGDFILFAYILALGK
                     FFSIIAALDTGSSFEGMGASREALFSLFAEPAFFILMGSLALLTGHTSFHEIFSALHL
                     GSYTTYAIAALGSFVLVMISMVENSRMPIDDPKTHLELTMIHEVMILDNSGFDLGLIL
                     IAGYLKFAIYAAIVFNLFVGTIHYEYSIPLFFFTQFILAVFMGLVESFMARFRMSHNP
                     QFIMALSSVALLIFFGVLLIIGKFI"
     gene            17955..18587
                     /locus_tag="PK049_06380"
     CDS             17955..18587
                     /locus_tag="PK049_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61220.1"
                     /translation="MINVLLITFLITLLYMSIANRLLTYLKVLAFQGVILFVVTFIQL
                     HEINTLNLILILLETIVFKSVAVPLFMAHILKRNKITREAEPYVPYFVSLIITTLIVV
                     TTILLSGTIKDPNLDKIFFVVALSTIFFGLYIIASRRKIITHVMGYLVIENGVFVLSL
                     AVGNEMPMLVNLGVMLDIFASVLILGIFLNKIGDVFKNIDVEELSNLKDY"
     gene            18607..20031
                     /locus_tag="PK049_06385"
     CDS             18607..20031
                     /locus_tag="PK049_06385"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOK61221.1"
                     /translation="MTILYLLGALSIGAGLFINRNKLVNYILTSLFIVLQWGFTIYAY
                     FHLNITEAEYFTFDSLGVLLLLVLSTISIPAIFHSHRYIDLHNEPAKSQSIYFSALII
                     LIAAIGLAYLANHIAVMWIFAEITTLAASALIYHHRNKLALEGTWKYVFICAVSVAFI
                     FIGILFLSLSLGKASSDSLSFQYMISRATTLNQFWLKLAFLFIFTGFTVKAGLVPMFT
                     AGVDAKDKAPGPIAALLSSVLMNMGVVGIFRTYKIIANTPIQYWANLVIGISAVLSIF
                     VATVYMIKVKNIKRMFAYSSIEHMGLVMLAISVGGIGYYAAILHIVLHAFVKSSLFFQ
                     YNQIYRVYQDKNIYHIGNYFKYNPTGAIVILLGFISATAMPPSGLFVSEFLIFRALFE
                     GHQIFILIAVLLLLTIIIWAFAKNILKVLFTPLTNFDESNVPIIKPWESYSQYALIIL
                     SIYLGLTPPDFFVQLIKESVMFLS"
     gene            20037..21536
                     /locus_tag="PK049_06390"
     CDS             20037..21536
                     /locus_tag="PK049_06390"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOK61222.1"
                     /translation="MNYISIKNNQTIPVADIPVLDYQQFSETNISLLSKHADRHCVNY
                     FGYIQGNSIKLVSCIADDTSHEIYISSCLVDSSKKLESMSSRLLCFEKFEREIHENFG
                     VVFENHPWMKPVRYPYNRADKSSVMDNYPFYSIDSEELHEVGVGPIHAGIIEPGHFRF
                     ICNGEQILHLEIQLGYQHRGLENLFLQKKKLLQRVTLAESIAGDTVAGHTTAFCYLWE
                     SLSGFDPKPEIDFYRTLALELERIAIHTGDLSAICTDIAYQLGSSVFGRLRTPIINFI
                     QEWGGNRLSKGLIRPGKTQFPFTKELGDRLNEILNDYEPDFNEMCKELFKLPSALSRL
                     ERTGTVSYDDVLNIGAVGMSARMSGLLRDIRTTHPYALYPQLNHKPILKHHGDVYSRA
                     QIRREEIWQSMAYIRQLLKDIPESKANYEMPIKLKPNAFSISLVEGWRGEICHCAITD
                     ADGDLAVYKVKDPSFHNWLALALAVRNNEISDFPICNKSFNLSYCGHDL"
     gene            21548..22300
                     /locus_tag="PK049_06395"
     CDS             21548..22300
                     /locus_tag="PK049_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase"
                     /protein_id="HOK61223.1"
                     /translation="MLDNLKILYHQGKQFIPDVRTAKVPGIFRGRPIINSHKVNVTEL
                     VDLCPMNAISSNPVSIDLGKCTFCGECALRFPEKIKFTTDYKISTNERERLIVKEGDD
                     FAIEVNPVTVRKEIRKIFGKSLKLRQVSAGGDNSCEWELNAANNVQFDMSRFGIEFVA
                     SPRHADGIAITGPITQNMAKALEIAYNATPDPKVIVLIGTDAISGGIFADSPALDRSF
                     LENHRVDLYIPGNPAHPLTIINGLLDLTRKKK"
     gene            complement(22308..23348)
                     /gene="rlmN"
                     /locus_tag="PK049_06400"
     CDS             complement(22308..23348)
                     /gene="rlmN"
                     /locus_tag="PK049_06400"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002935 - tRNA
                     (adenine-C2-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0070040 - rRNA
                     (adenine-C2-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOK61224.1"
                     /translation="MQKGETLVGKDLKQVEAWLSELGFPTHYGVKLTGWIYRKRIQNY
                     ADINDIPKHILKALKDEFGEVRGHIGYERYPGTDGSVKYLFTNAQGQHYESIFMDNGK
                     RKTLCVSTQAGCRMGCRFCMTGSIGFKGNLDAGSIVGQLLAIPESKSVNRLVIMGMGE
                     PFDNWVEVSQALEILTSAWGVAFGRSNITLSTVGILDGLERFLMNPPCNLAISLHSPF
                     PYERALLMPSEVKNPISRVVDILLKHPIPKPLRLSFEYVALGGINLSEQHAKAVCELL
                     KGLNYHINIIPWNEHSAASEFRKPTPVELENFTRWLNQHGALWTIRQSRGNEVGAACG
                     QMAGKRIDLKRI"
     gene            23759..>24297
                     /locus_tag="PK049_06405"
     CDS             23759..>24297
                     /locus_tag="PK049_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOK61225.1"
                     /translation="MSKLQVKLNNIYQFVDASDVLSLKNKVNDCQKLIEGKTGKGNDF
                     LGWVTLPAEITDDQLKSIEKVANELRSKSEFVVVVGIGGSYLGAKAVIEALGNSFSML
                     DNGGTKVLFAGNSICEDYHAELLKLLDKHTYSIVVISKSGTTTEPAIAFRLLKQHLEG
                     KVGKTEAASRIVAITDQSKG"
BASE COUNT         6805 a         5016 c         5133 g         7343 t
ORIGIN      
        1 agaaccaaag aaaatcaagg ctgaaaagcc cgacccgatg ggtaccccgc gggtgcaact
       61 gccgcgcgat actattcgcc acgcctgtgg cgtgactcat gtggtatcgc ttacaaaaac
      121 ccaccgccac gttccacccc cgacccattg ccgggtcggg cttttatgcc tctgcttgct
      181 tcttccgctc cattccctct cagggaaaag ggttggggtg aggtcgtatc ccacgaacaa
      241 cgagcaacct acacgaataa cttcatttgt catcctgagc gtagcgaagg atctctaatc
      301 gatctcatgt gacgtttcgc ttagctcaac attgcaatag cttcacgaac aacaaataac
      361 gcttccagcc ccgtaggggc gtaatatttg taactccatg gctactcctg ctctaaaacg
      421 atgcacgcta tacctttgcc aaagagttac actgaaacgt tgcatcgcaa cagaaaagta
      481 tggcaaagag cggtttacac aaaaaataac aaggattaaa ggtaaagaga tgcttcagct
      541 agctcagcat gacaagggag cagaaaggaa gatagggttt ttgtcatccc aaacaaggcc
      601 gagggatctc ctcaaaggat tgaagtgaaa agagcaataa gatgcttcgg cgaagtctaa
      661 ccctatataa ttcgggactc agcacgacag cgtgatgttt tgcttgcgct aagcatgaca
      721 ataactttaa ctaaacaaca ttaaaacagg aattgtaaca atgggcgaac tatttagttt
      781 aggcataata gaaaaaacat tgcacaaaat ggctcagatt tgcgataagc atcaaatctc
      841 agttattgtg tgacatttaa aaccttgttt acttcctgaa cgtcctgatg aactcctcaa
      901 cctcgggcac gccaccttgg taaatgagat aaccgttata gggttcacgt ggggtaatct
      961 catcgttaat gggagtaagc atcaggcgtt tcacctcctc ggggtcatcg gtttggccaa
     1021 gcacccaacc caatttgatg taggcagttt cggggagcat gttctcggcg gggatgattc
     1081 ccttggccat caggtcgcga ccggtgtcgt acacgtacat gtgggcatac ccccaaaggg
     1141 tttgaacggt catgaacatg tgaacaccct tctttttagc ccgctcaatg gcggggtata
     1201 gaggtttgtt aacgtggcca agtcctgtcc cggcaattat tataccccta tatccattat
     1261 cgaccaatga gtcgataata tcgggtttca tatttgggta gtagtacact atcgatactt
     1321 tttcttcaaa gtagggaagt attttggctt ttctgtcctt acggcggtgg ttgtaggtgt
     1381 ccttaatggg tttaacgcca tcctttaggc taacggttgc cagcggcgta tcgccaatgg
     1441 tacggaaggt gctgcggtac gaggagtgca ttttacgtac ccttgtgccc ctgtgaagga
     1501 atccgtactc gtcggaggtg gggccaaaca tacataccat tacctcggca atatcggaat
     1561 ggcctgctgt gtatgcagca tgcatcaggt taagcgctgc atcgctgctg gggcggtcgc
     1621 tacttcgctg ggagccaacc agtacaatgg ggattggtga gttttgaacc ataaaggtga
     1681 gtgccgctgc agtatggcta agggtgtcgg tgccatgacc aattattatt ccatcaatcc
     1741 cgttttcaat ctctttgcca atggctattg caagtgcctt gtactgctct gggcccatgt
     1801 tctcactgaa tactgcaaat aacttctctg ttgataggtt gcaaatatcg gctagttcgg
     1861 gtacagcgcc gtatagttcg ccaggcgaga atgctggtat tacagctcca gttcggtaat
     1921 cgagtcgcga tgcaatagta cctccggttc ccagcagctt aaccttggga aggttagggg
     1981 tgtatggaaa ctccttttct ggaattttat aggtggcctt gtagtacccc acctctttca
     2041 tggctttaat tgtaaaaatg tcgatgccga tattgtaacc cgttgctatc tttagcacaa
     2101 tgtgagtgtc atcgtcgttt tcggcacggg gtaaaacagt accctgaaac tcacctcggg
     2161 tagtttcaat gatggctttt ccccaaaccc taacgttgaa cttttttaga acttcaagcg
     2221 cacggccttt atatccctga aaaatatctt ccataatctt ttggttattc attgagtttt
     2281 tagctaattc gttgctttat ccttgagtgc aatacggcag ggtcaacatt ccccagtgct
     2341 tgcttatgca gctgtcccat tacccaattt atagcattct ccggcttatc ctttttggca
     2401 atctttatct ccttgaattt ttcaatgagg taatctacag gagcaagcag ttcttcctcg
     2461 gtctttcgct tgaagttgat actggttagt accgagttga aatccatgtt ggggtactgg
     2521 taaacaaccg gaagcataag cttagctatg gagtgatgca gcttgttctc tttaatgaaa
     2581 cggaatagtt ctacaattcg ggagtagtta aaatccttat gtttagggta tttcccttca
     2641 attcgtttca gcttatgccc aagcagtgaa cctacatagc gcgaagggaa tcccaaacca
     2701 acaatttcct cgataaccgg aaccaggttt cgccttaaca ggaagggatg ggcatcctca
     2761 ggaatttgca tttgggcaag ctgttggaaa cggtgccaaa tgtcgatagg taatcgctca
     2821 cgcaatttct caatataggc ctcgtccaga ggaatggggg ctgagtcggt atcggggtac
     2881 atgcggtcgg caccgggtag aacgcgttca aaaatggttg ttccatcggc aagcgggcgg
     2941 cgggtttcgc gtggaacccc ctcaaaggcc atgcggcacc gctcttcaat ggtttcaata
     3001 gctgttggga tatcgttggc ggggccccaa ataagtacaa ttgcgtcgtc gggtgaggct
     3061 ttgagcagcg atgctaaacg tgtagtgtcg ctttcagaaa gcggaatttc caaatcctca
     3121 ttactagtga tgttgggtcg ttcaatgcag gctataacct tgagcctatc agctagctca
     3181 ttggtgaagc actttccggg ctgggtgaaa tgcgagagta tgctatgaaa tgccggaagg
     3241 gcaattgcaa caaccttgta acccatatcg gcaaacctat ttgcaaccgc atgctcaatt
     3301 ccttgtgagt aggggtcgat ttcaatatgg cgcattaccc acttttcggg caaaggcaca
     3361 cgctccaaaa gcaattttct gatgtggagt agcgcccact gtctgaaaca ctcattatgc
     3421 gaaagcattg gtagccactt gttatgcgaa actcccttga tttccacacg tgttccaccc
     3481 tcgcagctta cgtttgtgtc ctgtcgggca gcaccaatac cagtacgaac cttgcctgta
     3541 ctacggttca gaaaccgaat atagtcagca gcctcgcgga gctcatcggg agtaaggcac
     3601 tgaggatgcg taacggtttc aattagcggc atgcccaacc tgtcggtttt gtatatgcga
     3661 cgatgcccaa catctgagat ttcacgacag gcatcttcct caatactgag ctggattagt
     3721 ccaacctttt tattctttag ctgaatctga ccctcaacac ccagaatggc ggttcgttgg
     3781 aatccggttg ggatggaacc gtccaggtat tgctttcggg taatgtgaac ctcgcccaca
     3841 atgtttaact tgcaaagcaa tgatatttca agagctattt ccagcgcctc tcggtttatg
     3901 ggaaaaggag gcgtgtcgtc aatttcataa gtgcaggtgg ttttgttgtt tatgcggtaa
     3961 acaatctcct ttcgggtttt aaactccata agggcagtac cgtcgtattc gcccatttcg
     4021 ctaagggttg gtcgcatatg gcgaataatt tccgcatggt agtcattatc gtcgtggtaa
     4081 atgcctgccg gacagcggca gaaaagtttt tgcttggttt tgagttgctg atgaacttca
     4141 agccccgatt tgaatccaat gcgttcataa tccttagggg tggcctcggc tcgggttaca
     4201 tagccaatag ccttcatggt ttcccggtag ttcttttgtg ggtcgaaatg cttaccgggc
     4261 gtttgttttt tggccatttt ggtttaggtg tgggttggtt aacagccttc taagttaaaa
     4321 aactgctagg aatttccaaa tgatttatca gtgctatgtg attgcttgaa aaattgcgta
     4381 taatcattaa gtaacagaaa attaactttt tagtggtttt ttgttgatga tttatcggaa
     4441 ttttaaattg aaatgattgc tagtggcata gtgtttgatg ctagttaaat gtttttctct
     4501 ggttttgcaa ttattttgaa accattcaaa atgtaatcta aaaccaaacc aaaatccaat
     4561 ctaaaaaaac gaccgcccct tttggggcgg tcgttttatg ctaaaatcaa ataaagtcag
     4621 gcttgaaaag gtgtggcaat gaagttaacc ctaatgaatt tcgatgggac agcagcggtc
     4681 tagaaacgaa aaagctcagg catagcctga gccaaatctc ttagaaagat tgaaacacta
     4741 ctttacagtt gcttccatta aagctttaat ggccttttcg agttgctggt cgcgtccctt
     4801 aggtgcaact gatggttcat tcattatcac gtaatcggga attgcttcaa cattttccat
     4861 ccaaacgcca tacatatctt tggcactgac cggtaccatg ccccagcgga tttgattatc
     4921 ctgaagtgtt tcccaaccag caaagctaca tgtgccagga acaggcattc caataagttt
     4981 cccaataccc aattgcttgt agccacaagc aaagcaacta ccatcgctgt agtttgcttc
     5041 gtttacaagt gctacagttg gttttgtcca tcggaaagtt ggctcggagc caagttcgcg
     5101 aacgtggtta tggtatgtga taaacttagt gccggtaaag aacatagcca aatccgaaac
     5161 caggtcgcca ccaccattga aacgggtatc aaccactacg cctgacctgt cgtaatattt
     5221 tcccatcatt tcaccataaa tattgcggta agggccatcg ctcatccctg gaatatggac
     5281 ataacccaat gtaccgttgc tcaacttttc aacctcatcg gcgttacgct taacccaacg
     5341 gcggtatagc aatcgggact cctgggatag gtttgtaggt ttcactgtaa cctgtctttc
     5401 ctttccatta cttagttctg ttaaggtaag tagaatatac ctgtcggctt ttcgattcag
     5461 gtagtatgcc caatccttat cagttccaat ggtattcccg tcaattgcag taataatgaa
     5521 gggtgtagag aggctaagtt ccttacggtc aagtggcccc ccaagcagaa tctcatcaac
     5581 cctaatgcct tgcccttgat aggttacatc gggtataata ccaagcgaag cggtttggtc
     5641 gccattgggg ttgttgaggt agaagcgtgc acctgaatgc gatacgttaa gttcgccaag
     5701 cagctcgctg agcagctctg caaactcaaa cccgtttcct atatgaggga ggaatttccg
     5761 gtagtgttct gccatgaaat tccagtcaat gccgtggtat gtgggtgtgt aaaatccttt
     5821 tcgggttctg ataaccacat gttcatacat ggcttgcctt tcggcatcgg tgttaaccgt
     5881 tagctctgct gctattttaa gtgcttcccg ttttcccttg tcaagatcaa ccttataaat
     5941 tttgccatcg gcaagcatga acatgttttt ctcgtcgtta tcccagtata gcttggcata
     6001 atcaacgtcc atctcaacaa ggatttttgc ctctttctcg cggatgttca tcgaccaaag
     6061 gttgtaacct ttatcgtagc gggcaaggta gtaaagggtt tcgccatcct tgctcagtag
     6121 ggcatcggca atatcggccg aggatgtagt taagcgtgct gtacgatact caatgccttc
     6181 ccagtcgaac ttcaatgagc tgtcaacctt ttcattatct tttttatcct tacccttttt
     6241 acctttttca tcgtcatcct ttttttgctt agtttctttc tgtttatcct caattgcttt
     6301 cagcaacttg tactcgtcct tgctaagcct gaatttatcc catgaatcgc gggtaaggaa
     6361 aagtgtatat acatcgcttt gacgcatacc gctattggcg tagctcctaa ggccatcgcg
     6421 agttgaaagc caaagaacct gcttgcctcc atttgaccag cgaggggtaa aatcgtcgta
     6481 accattttgt gtaagattcc tgaaaggttc agagccatcg gcgggtaagg caattatctc
     6541 gttgttggac atggttggtg agtattcaac caggagccag cggctatcgg ggctccagca
     6601 aaagcgttgg tcgccatcgc tcatgtagta tagttccttt gatgtgagta atgtttttgc
     6661 tgatttgctt tccaaatcta aaaccttaag ggttgttcta tcctcaatgt atgctaactt
     6721 tttgccgtcg ggtgagggta ggggttggta gttgtcgtat tcgttgccta tcagtagatc
     6781 ctcattaatt agggttgatg caaaaaagaa gggttcctcc gggcgaacac gcgaagcttt
     6841 aaatatttgc cagcggccct cccgctcgct tgcatataca aggcttttac catcgggagt
     6901 aaaggctaca aagcgttcct gttcaggggt ttgggttatt cgttttgtaa aagccccctc
     6961 taccgaggta acaaatacat cgcctcgtgc aatgaaagca acctctttgc catcgggcga
     7021 cacggaaagt tcgcgtatgt ttccgctaag cgatactgtt tgctcagggt tctgctggaa
     7081 gtcgcggttt atccttatat ctactttatt gctaattcca ttattaccca tagtgtatat
     7141 ggaaccatcg tagccaaagc aaagtgttcc gttttggctg acgcttaaaa aacgaaccgg
     7201 atgggtggta aagttggtaa cctgcttaac ctccgatgag ttggatagtg aaagtttgaa
     7261 tacattaaaa ttaccatcct cacggatgaa gtaaattact gaatcgccag gagcaaaaac
     7321 cggattacga tcctcaccct tatagtttgt gagctgattg tattcctggg tttgggtgtt
     7381 gtatatccag atatcgcgtg caatagaaga aaggtggtgc ttgcgaaact cattctcacc
     7441 gcctttttta tcgtgaaatg ctagaagctt tccatcgcct gagaccgata cccattctgc
     7501 gggaacgggt agaacctgat taactaaacc cccgttgacc ggaacggaat aaagttcagg
     7561 ttgcgatgcg gttgggtata ggcggctgga ggccatgtcg tatcgtgcgg caccgaatag
     7621 cacatgcttg tcgtcggctg taaaggcgta gggtaactca tcggctgaat ggtaggttaa
     7681 acgtttgggt tcaccacccc tggatggtac aataaacaca tccatattcc catacctatt
     7741 cgaggcaaag gcaatgtaat cgccgttgtg gctccatacc ggtaggtaat cgtgtgccgg
     7801 attagctgtc aatgcaactg cctcgcctcc gctggcagaa accgtgtaaa tatcaccctt
     7861 gtaaacaaat gcaatggttt ttccattagg cgaaatggct gggtaacgat accattctac
     7921 ttggtcttgg gaactccctg atattgatat gcttgcaaga gctagggcga gaattatttt
     7981 agtgaatttc attttggtat gttttaaaag ttcgtttagt catactctaa attaaaccgc
     8041 ttatccagtt aatggtttaa aatgtttagt tttatttaaa aattgataat tttgaagata
     8101 aaattaagga aaatgttctc gctcagtaac actgaacatg tttttgaagg aagaagcaat
     8161 gcctcgctcc gctgcgagtc cttaattctt tccattcttt ccaaaaaaat ggtagcaaag
     8221 tccctaattt tactctcaat ttttgccaaa aggttaaaaa tttttagaga ttttccaggt
     8281 aaacttttag taaaacgatt ttttagccac aattccatag atgagctaaa agaaatatct
     8341 acccaaaatt ttcacaatag aattctctcc tgtattttac cagtttcaaa cggaaatggt
     8401 tcaactgtca atttgttatc ggatttagct aacgataaat ataatattcc ggggttggaa
     8461 ttcctttttc ttaatgttga cgattaccta gaaccccgca ttcttagcct ggtatcaaac
     8521 gatggttttt tgaatcaggt cgataagaaa gcttttcaat cgtttactga ttatgttgat
     8581 accctatgcg aaattgccag tagaaataat aaatcgataa tcctatatac agccaatttt
     8641 gcaggttttt cagcaattca tgcaatagcc attgacttaa tgcaacggta taacacccaa
     8701 aaagtaacgg tatttttact tttccatttg tgccatctta actcacaaaa cagaatagtt
     8761 gaccttgtaa gaaggggctc aagccaaggt tttacccctg gtattgcaat tgccaagtat
     8821 ttaaccacag agtatgtgaa taaaaagttt gtcgataata gccacaacga ctctagctgt
     8881 catggtttta atcagaccaa gcgagtgttt tatgaggtaa ctaaatttct gctaaataac
     8941 atcgatcatg tttcagcatt actgattagc cacaaccccg ataatatact ttaccttaca
     9001 tcgcttatgg aggggtgtgg tgtagggcat agcaataaga atgttacctt tttgcaacgt
     9061 tacggaatgg caaaatcctt aacattaaac cttgccaaca ggggatataa tgttgcaaca
     9121 attataccct acggtaacct aactgataca ttacgatgga tgcatgggct ttatggcttt
     9181 gacccgtcgt taccaaatat ttttaccgaa cagcgttttg ccattttaag agagctaaat
     9241 aggcgcaaac tagaaaattg atgcattatg aaatttgata accaggacac tgtatatgga
     9301 atccactccc tgaagcttgt tttagcagta gtggtggtaa ttgttatagt attaatgtac
     9361 ttaacatcaa ttcccgatta tattgagagt agtattggaa ttagccaggg atgggtgata
     9421 gccattgttg taatacttta cctgatgttt ttcgggtatt ttatttggat ggatgctgca
     9481 tttatctcgt ataacgatga gggcaacaag ctggttattc gtactttcaa gctacgtcct
     9541 tggggtggta aaaagatatc gatggaaata ccacactcag aattctacaa gtatgagatt
     9601 acccacaaat ggccaaaaaa acagctacac atttatgtaa aaaaggggaa taagatcatg
     9661 aaataccctc ccgtttcggt tgtgtcgtta acagcagagc aataccagaa aatggagagt
     9721 tcccttaacc aactatcgaa agcataatgc atgggcgaaa gcgagttgca atatcgcata
     9781 gcaattgagc ttatcccaaa ggtagggagt ataactgcta aacgtttaat tgcctactgc
     9841 ggaagtgccg aggcggtttt tcgtcaatcc aaaaaagcgc tgcttaaaat acctggcgtt
     9901 ggcgaaacca ttgcagctga agtggtcaac cagaatgtgt tacaccttgc cgagaaagag
     9961 attgagttta ttgagaggta taaaattaaa gccctatact ttactgaccc tgaatatccc
    10021 gaaaggctgc gccagtgtga ggacggccca gtaatgcttt acgtgaaaac tcattccagc
    10081 ataaacttta attctgataa gataatcagc atagtgggaa cccgaaaggc aacggcttac
    10141 gggaagcaaa catgcgaagc cattgtaaat ggacttgccg ccaagggtca taacccaata
    10201 atcgttagcg gattagctta cgggattgat atcgtagctc acaaaagcgc attgaaaaat
    10261 aacctgcaaa cagttgcagt gctaggacat ggtctggata ccatttatcc tgtagcacac
    10321 agggcggtag ctaaggagat ggttgagcag ggagctctgg taaccgattt tatttctgga
    10381 acgccatttg accccaataa ctttctgagg cgaaatagga ttattgccgg catatccgat
    10441 gcaaccattg tggttgaaag cgccctggag ggtggtgcgc ttgtaacagc cgatattgca
    10501 aactcataca atcgcgatgt atttgccgtg cccggcaatg taacatcaac atactccatg
    10561 ggttgtaaca tgttaataaa acagaacaag gcagcactgg tggagtcggc cgacgatatt
    10621 gagttcctgc tgggttggag cactaccagt aaaacccaaa ccgttaagca gtacacaata
    10681 aactttgatc attttaccaa cgaggagaaa aaaattatta actttctgaa ggaaaaaggg
    10741 gaagaaaccg ttgacctgat tgcccttgga acaggacttc cggtttcaac cgttttatcc
    10801 tcgcttctta atttggagtt ttcgggagta gtaagtagca aaccaggcaa agtattcgcc
    10861 ctaaaaaact aatgcaatga gcaaacaacc aaaaccaaac aggataatgg agctgtataa
    10921 aaaggttcag cgtttcctgc ttcatggtat atggagcatg aaccttgatg agcttccgcc
    10981 taagctgaaa tggccattta agtatttacg ggtgctcttg cttgcgctca agggtttctt
    11041 tgaggataag gtaattgtta aggcttcggc tttaacatat tacacactaa tgtccattgt
    11101 tccaattttt gcaatggcat ttggaattgc aaaggggttt gggtttgaac gataccttga
    11161 acagcagtta actacccagt ttaagggaca ggaagaggtg attaataagg ttattgtgtt
    11221 tgcaaactct ctactagcac gaaccggggg cggcttaatt gctggaattg gtgttgtact
    11281 ccttttttgg tcagttatca acgtgctatc cagcattgag catgccttta acgatatctg
    11341 gcaggtagaa aaacctcgca gctgggtacg caagtttacc gattatcttt ccattatgct
    11401 catttcgccg attctactta ttgcttccgg gagtattcat attttccttg ctactacagt
    11461 gacaagcatt gctgaaaagc atgagcatgt gagctacatc agcccattcc tgattgatat
    11521 gctccagttt ttgccgtatc tattaatttg gatacttttc agttttattt ttatagctat
    11581 tcctaacaca aaggtttcct acaaatccgg tattgttgca ggaattattg ctggttctgg
    11641 ttttgtaatt ctccagtggt tgtatatcac ccttcaaatt ggggtttccc gatataacgc
    11701 gatttacggg agttttgcag ctttaccctt attccttttc tgggttcaaa taagctggca
    11761 aatcgtgtta tttggtgctg aaatatcttt tgcataccaa aatgtcgata tgtatgaatt
    11821 tgaaagggaa acaacccata taagccataa aaatcggaag ttgttagcct tgctggtgct
    11881 aagcaccatt gttaaacgat ttatgaatgg agagaaaccg gctacaaacc ttgaactatc
    11941 tgttaatcta aaaatacccc aacgactcat gcgcaacctc ctcgacttga tggttaactg
    12001 tgagctgcta aacgaggtgg taattaccga gagtaaagat attggttatc agcctgcact
    12061 tcatgttgaa cacctaacaa ttgcttttgt tgaggaaaaa ctcgatagct atgggtttga
    12121 gattgaaccc gatatcccag agttgaagca ggtaaaacag gtttgcacaa gttttaccga
    12181 gcgttaccgc gaattgaccg ctagcacttt gataggtagc atttagtcta ccagttttgc
    12241 cagtggatgt tttctacttt aaacttttcc ttgggttgat gctccccttt aggaaaacca
    12301 attggtataa cgttgagggg taaaacgtta tcggggagtc ctaaaacttc ctttacaatt
    12361 gcaaccctat cggggcgggg gtaaacgcct gtccatacag cccccaaacc cattgcctca
    12421 gcggcaagca gtatattttg cgtagcagca gagcagtcct gaacccagta tccttcaggg
    12481 ctatcgcctg ctttggaaag gtcgccgcaa acagctattg cggcaggtgc ctcgaaaagc
    12541 attttggcgt agggtaaacg ctcagcaaga gtatccaggg tggcccggtt agtaacaata
    12601 acaaatgccc atggttgaag gtttcttgcg gagggtgcag ccatacccgc acgggcaagt
    12661 tcttcaagta gttcattgct tacttcctgg ccagtgaagc tgcgcacgct tttgcggtta
    12721 agaataattc taagtgtatc attcatctcc ataatattta tcatttatgc tagaagatct
    12781 attttatgaa agcctaaatt tatataaaac taagttaaat aaaatgaatt atgggaaata
    12841 acgtatttca ttatttcgtt actttgcacc ctgaatgaag aattactaat aataggtgca
    12901 aatctgcttt ttaagaatta catagtgtta aaattctcat tcgatagtat agatttgaaa
    12961 tgtaaacatt ggggggaacc tatatcacca agggaaagtt atagtatctt tttttattct
    13021 gaatttggta acttgttatg acttatagca atagcaaaaa aagcattagt ggaatagtag
    13081 caattgctac aatagtagtt gttgcattgg ttagcagttg gatggctttg tcagcactga
    13141 tgggaaatgc actggcgtac cagttaaatt tagggctagc aataggatca gtcccaataa
    13201 aaatagatgg gctttccgct tggtttctgt taatcatcaa ctttacctca ataaccgggg
    13261 cgatctatgg gctcggatac ctaaaaggat actcaaaccc cgcagcaaag ttgaatttgc
    13321 actggataat gtttgccata ttccatcttt ccatgatttt ggtttgcatt gtacagaatg
    13381 gatttgtatt ccttgtgatt tgggaaataa tgtcgttgtc ttcggcaatg ctagttatgt
    13441 tcgatgggca taatcccaaa acgcttaagg ctggcataaa ctaccttgtg caaatgcaca
    13501 tcagcatagt atttttaacc gttggtttta tctggttata cgctgttact ggttcattta
    13561 atttcgattc gttacaatca ttcttcatcg gcaataataa catatggtta ttcctagtat
    13621 tcttcactgg ctttggactt aaggctggat ttattccact acatagctgg ctaccacatg
    13681 ctcacccctc tgctccttcg catgtatcgg gcgtaatgtc tggtgtgata gttaaaatgg
    13741 gtatttacgg tataatcagg gttgcctcct acctcacaaa caatcatcta cttttaggtg
    13801 aaatagttat ttcaatctcc gtactcactg gtatctatgg aattctaaat gccgctgcac
    13861 accgggattt taaacgcatg cttgcgtact gtacaatcga gaatatcgga ataattggaa
    13921 ttggcattgg aattggcctc attggcatta gtataaaatc tgacataata tactttttag
    13981 gttttggagg agctctgctc catgtgctga accattcctt gtttaaatcg ctgctattct
    14041 actccgcggg ctcggtttac cagcaaacgc acacaaggga tatggataag ttgggcggac
    14101 tgtcaaagta tatgcccaaa actgcatttc tatttttaat tggagcagtt gcaattgcag
    14161 gacttccacc tttcaatggt tttgtatcgg aatttataat ttacagcgga ttggtgaatg
    14221 gctttggcac tggcgataat ggattaacaa ttctacttat cctatccttt gctggactga
    14281 gcataattgg aggtttgtcg ctattgacat tcgcaaaaac ttatggcgtg atattcactg
    14341 ggacacctag aactagtctc gatcataaac cccacgaagt atcaaaccta atgatagttc
    14401 cgcaattggc aattattgcg gccatgctaa gcgttgcatt tttcccatca ttctatgtaa
    14461 acacaataaa cttcataatt aaggatttct caaatggatt accaataaac ccagcatttt
    14521 caagtgattt ctcgaacacg ctgaaatcta taatggtatt ttcgctcgca gtctttgccg
    14581 tgatacttgt agtatggggc atcaggaaaa tatcaacaaa aaacaaatcc attttagcag
    14641 acgaaacatg gggatgcgga tatacaaatc caacgcctcg catacagtat accggaaagt
    14701 cgttctcgaa acagttggga aaaatattca acttcttttt gattgaaaag aaaaagtata
    14761 acgaactaaa tagcggggaa atcttcccgc ataaaaggca gtatttctcg ttctacaccg
    14821 atttcttcga aactcgtttt ataaatctga taactcagcg gattcttttc accgcaaatt
    14881 acttcagatt tattcagaat gggcgaatac agtcgtacgt gctgtatgga atagtattca
    14941 tattggccat ttttattctt tcagcactaa acctattctc attttaaagt gtattgatgg
    15001 aagcattaaa attatttcta actataccgc taattgcctt tctggcaatg ccatttatga
    15061 gtataaaatg gcgaggaata gttgcatacc ttgcagtaat acttaatgct attctatcat
    15121 cgtatatcgc tataaacgtt ttaggtggag aaaactacca attcatattc tcaggcaacg
    15181 cagtaacagg cgaaattcca gttagaattg atgcgctctc ggcatggttt atattaataa
    15241 tcaatttcac cattttaacc ggaggcctgt acggaattta ctacatgaga gagtatttaa
    15301 ccatgcgcaa cagcatcacc ctacacgtta ctgcgttcat cttgctacat actgccttgc
    15361 tgagcctttg cggattgcaa aattcgatag tatttctact aacatgggaa ataatggctc
    15421 tcagcgcatt tgtgctggtt atatttgagc acaacaaggt ttccacaata aaagctggaa
    15481 ttaatttctt gatacaatca catgtatcga taatattttt gctactcgga tttgtttggg
    15541 ttgcagcaaa aactggcagt tacgacttct tatccatatc gcaatttaca catgagcatg
    15601 ggaactcggt ttctctggtt ctgttcatat tcctttttat cggatttgcc attaaggctg
    15661 gctttgttcc atttcacaca tggctgccct atgcacatcc tgcagctcca gcccatgttt
    15721 cgggaataat gtcaggggta ctgataaaaa taggtatata cggaatactc cgagttattc
    15781 tattgcagaa taccaacctt gaggtaattg gatatataat tattgcattt tcggcaatat
    15841 cgggaatata tggagttatg ctggccatcc tgcaacacaa tttaaaacgg ttattggcat
    15901 accatagcat agagaatatt ggaataatag gactaggaat tggaattgga tgtgttgggc
    15961 ttggcaccca gaatttaatc gttgcatcgc ttggttttgc aggtgcactc cttcacacgc
    16021 ttaaccactc gctatttaag tctcaacttt tcttttcggc tggcattgtt taccaagcaa
    16081 cccatactgt tcaaatcgaa aagcttggtg gcttaatcaa gaaaatgcca caaacagcat
    16141 tcctattcct tattgctgca cttgcaatat cgggacttcc accatttaac ggttttatat
    16201 ctgaattttt aatatacagt ggattgtata attggcttcc aagtgcagac agcggaataa
    16261 taattacagt aattttttcg attcttgcac ttgttctgat cggcggactg gctttacttt
    16321 gctttaccaa agcatttggg atagttttcc ttggaaatcc tcggcatcaa tttaaacatc
    16381 aaatttccga ggcatcgttt ggtcaaattc ttccacttta ctttatcacc gcactaattg
    16441 ttgcaattgg gctattccct aatgcgtttt taacaatagt tagtaagccg ttgaaccttt
    16501 tcatcgcact agatcaaaac aataccagca caagcatagg aggtaattct gagatactcc
    16561 agatgatatc attttcattt gcattgttgc ttgtatttac tgctataata ttcggtatta
    16621 gacaaatggt acaacgcaaa catgctattt ctgctgatga aacatggggc tgtggctata
    16681 ccggtgaaac aaaaaaaatg cagtacactg caaactcctt tgctcggtca tattacaaac
    16741 tattccgatt tatcctttta gttttcaaaa aagaaaaaac tgtggaaaac gtttttccaa
    16801 ggcatgctga atttgaatcg agcccatacg ataagattga aaagttactg attgacaaac
    16861 ccattcaatc aatacggttt ttcatgggta agtttacctt tctgcagaat ggaaggctgc
    16921 agtactatat catttatggg ataacgttta ttcttgcaat aattgctatt ccattactat
    16981 acgagaaact gttacaactt attgaaatac tcaaacattt ataatcatgt taagtgcgat
    17041 actgataatt ttagcggcaa tattctatac gggtataatt aacaggacaa aaagtatagc
    17101 atcgggtcgt aaaggacctg gactatttca gcacctaagc gatgtcatta gattatttaa
    17161 gaaaggggca gtttacagca aaaccaccag ttttattttt caaattgctc caaccatcta
    17221 tttctctacc gtagtaattg ctatgctact tgtgccgatt ggtaattcta aaggattatt
    17281 tagctttcag ggggatttta ttctttttgc ctacatactg gctttaggta aattcttcag
    17341 tataattgca gcgttggata ccggtagcag ttttgaggga atgggggcaa gccgcgaagc
    17401 attattctca ctattcgcag agcctgcatt ttttatcctt atgggctcct tagctttgct
    17461 tactggacat acctctttcc atgagatttt ttcagcactg cacttaggtt cttacactac
    17521 ttacgctatt gcagcattag gttcttttgt acttgtaatg atttcaatgg ttgagaatag
    17581 tcgcatgcct attgacgatc caaagactca ccttgagtta accatgattc atgaggttat
    17641 gatactagac aatagcggtt ttgatttagg gttaattctt attgctggat atttgaagtt
    17701 tgccatatat gcagcaattg ttttcaacct ctttgttgga accatccatt acgagtattc
    17761 tattccattg ttctttttca cccagtttat tttagctgtg tttatggggc tagtggaatc
    17821 gtttatggca cgtttcagaa tgagccataa cccacagttt attatggcac tttcatcggt
    17881 ggcactgctt attttctttg gcgtgttact tataattgga aaattcattt aaaccagtaa
    17941 taaaagttaa cgcaatgata aacgtactgc taataacatt tctgataacg ctgctttata
    18001 tgtcaattgc taatcggcta ctgacatatc tgaaagtgtt agcattccaa ggggtgattt
    18061 tgtttgtcgt gacatttatc cagctgcacg aaatcaatac cctcaaccta atattgattt
    18121 tgcttgagac tatagttttt aagtcggttg cagttccgtt atttatggca cacatcctaa
    18181 aaagaaacaa aataacacgc gaggcagagc catacgttcc atactttgtt tcattaatta
    18241 tcacaacatt gattgtggta actacaatcc ttctatctgg aaccattaag gatcccaacc
    18301 tcgataagat attctttgtt gttgctctct ccacaatttt ttttgggcta tacatcatcg
    18361 cttcgcgcag gaagatcatc acacatgtta tgggttacct tgtaatagaa aatggtgttt
    18421 ttgttttatc tctagccgta gggaacgaaa tgccaatgct tgtaaacctt ggtgtaatgc
    18481 tcgatatttt tgccagtgtc ctgattctgg gcattttctt aaataaaatt ggtgatgttt
    18541 tcaaaaacat cgatgttgaa gaattaagta atcttaagga ctattaatta acctaaactc
    18601 atgataatga ctattctata cttattaggg gctttatcaa taggagcagg actatttatc
    18661 aacaggaata agttggtcaa ttatattctt acctcattat tcattgtttt gcaatggggt
    18721 tttacaattt atgcatattt ccatttaaat ataactgagg ccgaatactt tacttttgat
    18781 tcgcttggag tcctactatt gcttgttttg agtacaattt caattcctgc aatatttcac
    18841 agccatcgtt acattgacct acacaatgaa cctgccaaat cgcaatcaat ttatttttcc
    18901 gcgctgataa ttttaattgc agcaatagga cttgcatatc ttgcaaacca tattgcagtg
    18961 atgtggattt ttgccgaaat tactacactt gcagcatcgg cattaattta tcatcaccgt
    19021 aataagcttg cccttgaggg tacctggaag tatgtattta tatgtgcggt tagtgttgcg
    19081 ttcatattta taggaattct ttttctaagt ctttcattgg ggaaggctag ctctgacagc
    19141 ctttcatttc aatacatgat atcccgtgcc acaaccctta atcaattttg gctaaagctg
    19201 gcattcctat ttatttttac gggctttaca gtcaaggcag gccttgttcc tatgtttact
    19261 gcaggtgtag atgcaaaaga taaagcccca gggcctattg cagcactatt atcgagcgta
    19321 cttatgaata tgggtgtagt tggcattttt agaacttaca aaataattgc taatacacct
    19381 atacaatatt gggccaacct tgtgatagga atttcagctg tcttatcaat ttttgtggct
    19441 actgtttata tgataaaggt gaaaaatata aagcgcatgt ttgcttactc aagcattgaa
    19501 cacatgggat tggttatgct tgctatatcg gtaggtggaa tagggtacta tgctgcaatt
    19561 ttacacatcg tgcttcatgc ttttgttaaa tcaagccttt tctttcagta caaccaaatt
    19621 tacagagtat atcaagataa aaatatttac catataggta attactttaa atacaatcct
    19681 actggggcta ttgtcatttt gcttggattc atcagtgcaa cggcaatgcc accttcgggt
    19741 ttatttgtga gcgaattcct tatcttcaga gcattattcg aagggcacca aatattcata
    19801 ctcattgctg tgctattatt gttaacaata ataatttggg cttttgcaaa aaatatactc
    19861 aaggtgcttt ttacaccttt gactaatttc gacgaaagca atgtccctat aataaaacca
    19921 tgggaatcct actcgcagta tgcattaatc attttatcga tataccttgg gttaacccca
    19981 cccgattttt ttgttcaact aattaaagag agtgtaatgt tcttatcata aaagacatga
    20041 actatatatc aataaaaaat aaccaaacta ttccagtggc tgatattcca gtgttggatt
    20101 atcaacaatt cagtgaaaca aatatttcgt tgctttctaa gcatgccgat cgacattgcg
    20161 taaactattt tgggtacatc caaggcaata gtatcaaact tgtaagctgc attgccgatg
    20221 acacaagcca tgaaatttat ataagctcgt gcctggtgga ctcatccaaa aaattagaat
    20281 cgatgtcgag caggctgctt tgcttcgaga agtttgagcg ggaaatacac gaaaattttg
    20341 gagtagtttt cgagaaccac ccctggatga aaccggttcg atacccctac aatagagctg
    20401 acaagtcaag tgttatggat aattacccat tttactccat tgacagcgaa gaacttcacg
    20461 aggtaggggt agggccaatc catgcgggca taattgaacc cggtcatttc cggtttattt
    20521 gcaacggaga gcaaattctc catctggaaa ttcaacttgg gtatcaacat agagggttag
    20581 agaatctttt cctgcaaaaa aagaaactgc ttcaacgggt aactcttgcc gaaagcattg
    20641 ctggtgatac tgttgctggc catacaactg ctttttgcta cctttgggaa agtttaagtg
    20701 gttttgaccc aaaacctgaa atcgactttt acagaaccct agcattagaa cttgaacgaa
    20761 ttgccataca tactggcgat ttaagcgcca tttgcaccga tattgcctac cagttgggta
    20821 gttctgtttt tggccgatta agaactccaa ttataaactt tattcaggaa tgggggggga
    20881 acaggctttc aaaaggtttg atacgaccag gaaaaacaca atttccattt acaaaggaac
    20941 ttggcgatag gctcaatgag atattaaatg attatgagcc tgacttcaat gagatgtgca
    21001 aggaactatt taaactgcca agtgctctgt caagacttga acgtaccggc acagtttcct
    21061 atgatgatgt tctgaacatt ggggcagtag gaatgtcggc acgcatgagt ggcctcctaa
    21121 gagatatcag aaccactcat ccatacgctt tatatccaca gctaaaccat aagccaatcc
    21181 taaaacacca tggtgatgtt tactcaaggg cacaaattcg cagggaagag atatggcaat
    21241 ccatggccta tattcgtcag ttgctaaagg atattccgga aagcaaggcc aactatgaaa
    21301 tgcccattaa acttaaacca aatgcattct caatatcatt ggtagagggc tggaggggtg
    21361 agatctgcca ttgtgccatt acggatgccg atggagatct tgccgtttac aaggtgaaag
    21421 atccctcatt ccataactgg cttgcgcttg cacttgcggt tcgtaataac gaaatctctg
    21481 atttcccgat ttgtaacaag agttttaacc tatcatattg cggacacgac ctataaaact
    21541 atttgccatg ctagacaatt taaaaattct ataccaccaa ggtaaacaat tcatacccga
    21601 tgttagaaca gcaaaggttc ccggaatatt taggggaaga cccatcatta actcacataa
    21661 ggtaaacgta acggaattag ttgacctttg ccctatgaat gcgattagca gcaatcctgt
    21721 ttcaatcgac ttgggcaagt gtaccttttg tggggaatgc gcactaagat ttcccgaaaa
    21781 aattaagttc actacggatt ataaaatttc aactaacgaa cgggagagac taatagtaaa
    21841 ggaaggcgat gatttcgcaa tagaagttaa ccccgtaaca gttcgcaaag agatccgaaa
    21901 aatctttggg aaatcgttaa aacttaggca ggttagcgct ggtggcgata atagttgcga
    21961 gtgggagtta aatgcggcca acaacgttca gttcgacatg agccgtttcg gaattgagtt
    22021 tgttgcctcg ccccgccatg ccgatggcat agcaataaca ggtccaatca cccagaacat
    22081 ggcaaaagcc ctagagattg cttacaacgc aactcccgat cctaaagtca ttgtcctaat
    22141 tggtactgat gccataagtg gtggtatttt tgctgatagc ccagccttgg atagaagttt
    22201 cctggagaac cacagggttg acctgtacat accaggaaac ccagcacatc cgcttacaat
    22261 tattaatggg ctattggatt taacaaggaa aaagaaatag caatcgttta aatacgtttt
    22321 agatcaatgc gtttgccggc catttggcca caggccgccc caacctcgtt tccccttgat
    22381 tgacgtatag tccaaagcgc tccatgctgg tttaaccagc gagtaaagtt ttccaactcc
    22441 acaggggttg gtttcctgaa ttcgctagca gcagagtgct cgttccaggg aattatattg
    22501 atatggtaat tcagcccttt cagcagttca cacacagctt tggcgtgctg ttcactcagg
    22561 ttaattcctc ctagagcaac atactcaaac gagagccgta aaggctttgg tatggggtgt
    22621 ttgagtagta tgtcaaccac tcggctaatc ggatttttta cttccgatgg catcagcaac
    22681 gccctttcat agggaaaggg cgaatgaagg ctaatggcca gattgcatgg cgggttcatc
    22741 aagaacctct ctaaaccatc aaggatacca acggttgaga gcgtaatgtt tgaccttcca
    22801 aaggcaaccc cccaggccga tgttaaaatc tccagcgctt ggctaacttc aacccagtta
    22861 tcgaaaggct cacccatgcc catgataacc aatcggttta cacttttact ttcggggatt
    22921 gctaacagct gtcctacaat actgccggca tccaaattac ctttgaaacc tatactcccg
    22981 gtcatacaga acctacatcc catgcgacac ccggcctgag ttgacacgca aagcgtttta
    23041 cgcttgccat tgtccatgaa aattgattcg tagtgttgcc cttgtgcatt ggtaaacagg
    23101 tattttactg aaccatcggt tccgggatat cgctcatagc cgatgtgacc acgcacctca
    23161 ccaaattcat cttttagcgc ttttagtata tgcttgggaa tatcatttat atcagcataa
    23221 ttctgaattc gcttacgata aatccatcca gtaagcttaa ctccatagtg ggtagggaat
    23281 cccaattccg ataaccatgc ttcaacctgc tttaggtctt taccaactag cgtttcccct
    23341 ttctgcattt aggaattagt ttaaactgca aatttagctt ttgttataat ttttatttga
    23401 ataagtgtgg tttcagcttc aaacggttga aatatagaat ataggcaatt tattatcaaa
    23461 ccatacttga aatcaaaatt actttgattt taaattcttt gtaacttcgc ggagcaatga
    23521 gaaatcttct aaaaatagta ttccctgttg cggcaatttt tttattgctg cttgcttacg
    23581 ggtgcaatcg gagggtggca cccaaaagcg ctaaggttga taaaacccga aagctaaagt
    23641 gcaggtgtgg taaaaccaaa tttcccaaaa gccatttcca ggttactatg accagttttg
    23701 ataacaggtg atgctagtta atttaacaat gattggaata cataaaccat agacaaagat
    23761 gagtaaacta caggttaaac ttaacaacat ttaccagttt gttgatgcta gcgatgtgct
    23821 ttcgcttaag aacaaggtaa acgattgcca aaagcttatt gaagggaaga ctggaaaagg
    23881 gaacgatttt ttgggctggg taaccttacc tgccgaaatc actgacgatc agctgaaaag
    23941 cattgaaaag gttgccaatg agctccgttc caagagtgag tttgtggtag tggtaggtat
    24001 tggcggctct taccttggag caaaggctgt gattgaggcc ttgggcaata gcttttccat
    24061 gctcgacaac ggtggcacaa aggtgctttt tgcaggcaac agcatttgtg aggattacca
    24121 tgccgagctt ttaaagttac tcgataagca cacctattca atagtagtca tttcaaaatc
    24181 aggaactacc acagagcctg ccatagcatt ccgcctgtta aaacagcacc ttgaggggaa
    24241 ggtggggaaa accgaggcag catcgcgtat agttgcaata accgatcaat ctaaggg
//
LOCUS       DAOSAL010000035        23701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_1540_length_23701_cov_48.821492, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000035 DAOSAL010000000
VERSION     DAOSAL010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 23701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23701
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1540_length_23701_cov_48.821492"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..2381)
                     /locus_tag="PK049_06410"
     CDS             complement(99..2381)
                     /locus_tag="PK049_06410"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOK61226.1"
                     /translation="MKSKYSLKYIVQGSLLGLTVGLVFLAIAYVVGVLTNDLALTPGG
                     IQDLHRIMPLFYIFDFLPIILGAVGFFVYQKLSLRINDFETTLQENISRERKLYRFVE
                     KLREGDVEAEYTPDDTDALGKAIVSLRNDIRQSRMEEEKRKKEDEQRHWAAEGMALFG
                     EILRQNNDNIEELTFQVIRNLVNYVGANQAAFYLLEDDDETDIHFRMTACYAYERRKY
                     ADRIIPWGDGLVGACALEKEKIILKKIPQGYLSITSGLGKANPKFLMLIPLKMNDVVH
                     GVIELASFNPFENYQEEFIDKVAESVATTIATVKINIRTAKLLKESREQAEELALKEE
                     QMRQNMEELQATQEEASRQSAKFISFSNSVNHTMIRADFDVDGTLLYANTKFLQKLEY
                     STNSEVEGKHISMFINKKDRLWFDDIWSTLSKGGKHFEGDMKLLTRNGNDLWTIATFT
                     CVRNDAGGVDKILFLGIDTTDQKKLSLDYEGQISALNRSSIKAEFSPTGDLTEANEKF
                     LLTLSYNLNAAKEKSIFEFVPSAERKNIERVWDDVIHGIPYEGQFRMLTSNGDERWFR
                     CTFTAVNDMYNEVAKVILIANDITREKNMEFETQRQTEQLKKQEEQLRQNEVELNKKL
                     REAKEEVRNQFKEIEKVKIRNEKTLEGFLDAIITTDHDGIVQFFNKAAEELFEVKREQ
                     VIGQSVRILFPDNAAENDEFLDAYLDPNKEKITGQRKEITILTKSGKELPVLMLITEA
                     KVGREITYTAFIQNISVDLF"
     gene            complement(2492..>2689)
                     /locus_tag="PK049_06415"
     CDS             complement(2492..>2689)
                     /locus_tag="PK049_06415"
                     /inference="COORDINATES: protein motif:HMM:NF013502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HOK61227.1"
                     /translation="GMGDDGAKGLLEMKEAGAYTIAQDEKSCVVYGMPREAVKLDAAS
                     TILPLQDIANHIIQRVKTRLS"
     assembly_gap    2690..2789
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2790..3547)
                     /gene="cheB"
                     /locus_tag="PK049_06420"
     CDS             complement(<2790..3547)
                     /gene="cheB"
                     /locus_tag="PK049_06420"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HOK61228.1"
                     /translation="MLNRIKVLIVDDSALVRQTLLEIISSDSELEVIGTAADPYFAAR
                     KMAEVTPDVITLDIEMPRMDGLTFLRKIMTQHPIPVVIISSLTSRATEMGMRALELGA
                     VELITKPQVNTKAFLEESKVIICDAIKAAARSRIARRKILTPPIVEPKLSADVIIPKQ
                     VVSSTPLTTTEKIIAVGASTGGTEAIATFLQAMPVDCPGIVIVQHMPEKFTTSFANRL
                     NDICKITVKEAQNGDALLPGQALIAPGNYHMLLKR"
     gene            complement(3600..4109)
                     /locus_tag="PK049_06425"
     CDS             complement(3600..4109)
                     /locus_tag="PK049_06425"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HOK61229.1"
                     /translation="MDHFKSHYLYPASLFADKEPHVVTTILGSCVAVCLYDSVLNQGG
                     INHYMLPLWNGQGLASPRYGNIAIERLIERMISLGSKKENLKAKVFGGGEVLETNLDN
                     FHIGQRNIAIALELLAEHGIPIIAKSVGGNQGRKIEYNTATGEVRHKFIQKTLNSNEV
                     KSISTKKAD"
     gene            complement(4119..5030)
                     /locus_tag="PK049_06430"
     CDS             complement(4119..5030)
                     /locus_tag="PK049_06430"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOK61230.1"
                     /translation="MTKKPEELTDNELLAELQRRFELNKKALDELKVLNAELKQVNKK
                     LEESEALKSHFISNITNEIINPFTSIIGLSKAILSVDKENWKKVISMVALIHSEAFNL
                     DFQLRNIFVAAKIEAGEIQPEIMQVDVNNLLESVIESFKYELRKKNLKVEYSYNTSTN
                     LVNPKIFKTDPEKLKLIIANLLSNAVNFSFEGGIIEIITSRGVNKLTICVRDHGIGIS
                     QENHKIIFDRFKRLDTGINSLNRGHGLGLSVNRAIIDMLNGSIEIESELGKGACFTIS
                     IPEPNVDVDDLATDANEMFFDQDAEIF"
     gene            complement(5042..5896)
                     /locus_tag="PK049_06435"
     CDS             complement(5042..5896)
                     /locus_tag="PK049_06435"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HOK61231.1"
                     /translation="MQANEFVDYYKLQLTDDEFKRLSALIYKESGIKLPPIKKVMLQC
                     RLQKRLKHLNIGSFKAYIDYLFSREGFDSEIIHMLDVVSTNKTDFFREPAHFEFLTEE
                     LLPEYYKANKSGTLKVWSAGCSSGEEPYTLAMVLFEFAESHPGFNFTIYATDISTRIL
                     QTAIDAVYKEEKVEGIPLTLKRKYMLRSKDRTNPTIKMAPHVRSKVTFARLNLMDSSY
                     DTKDLFDIVFCRNVLIYFDRNTQEAVINKLCQRIKPNGYLILGHSESILNMDVPLKQV
                     KPTIYRKV"
     gene            complement(5908..6423)
                     /locus_tag="PK049_06440"
     CDS             complement(5908..6423)
                     /locus_tag="PK049_06440"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOK61232.1"
                     /translation="MDTENKNQIKSYLTFKLGNEEFAAHVSKVLNILEMTPITKVPKA
                     PDYLKGVINLRGAVLPVIDTRIKFGMPETEYTNNTCIIVLDIDVDGESVHVGAIVDSV
                     QAVLEIDNSQIMPLPTLGSRYKSEFIIGMAKVEERFIIILNMDAIFSVDEITSIVDSN
                     QSVAEGTDAQQ"
     gene            complement(6443..8470)
                     /locus_tag="PK049_06445"
     CDS             complement(6443..8470)
                     /locus_tag="PK049_06445"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HOK61233.1"
                     /translation="MDSFRKKFIEEAAELIDKLEVSLLELEKSPNDEALIQQVFRVMH
                     TLKGNSAMFGFDLIDSFTHNLETIYDMIRNGQLEVSKDILNITFSSVDHLKAMLDEQN
                     YEDPDFKTVHNGLMGKINRIINPSNAETEAESAKSSNEKSESLSTYYILFEPNENIFK
                     NGTNPLYLLDELCSLGEHKVFAHFNRLPGIKEITPNLCYTYWEVLLTTDQDVNAIHDV
                     FIFVESDSKLEIQKLAEENLLADKLFVEEVSQLAEVQKDVGLVTIQRLATKAAARINK
                     EKAKQFTKERIATKDKTAASIRVSSDKLDHLMNLVSELVTTQARLSLFAEQTNVPGLA
                     PIVENVHKLTRQLRDIAFSIVLIPIENLFGRFQRLVRDLSAELHKEVDFIVEGADTEL
                     DKTIIENLSDPLMHIIRNSMDHGIEDARVRITNGKPAKGKIHLKAFYSGASVIIQVSD
                     DGAGIDPEIIHAKAISKGLVPSDKKMSKKEILDLIFLPGFSTAKKVTDVSGRGVGMDV
                     VKKKISDIRGEVEVESEVGKGTTITIKLPLTLSIIDGLLVSIDSSPFVIPLSSIDKIY
                     AVDKESIYSSFNNLVTLDGTQIPFFSLRKEFNLQPSSEELEQVVVVNFEDNRVGLVVD
                     YVIGEYQAVLKPLGKHYKSHDIFSGATILGDGTVALVMDTNKIIKMFATSK"
     gene            complement(8470..8835)
                     /locus_tag="PK049_06450"
     CDS             complement(8470..8835)
                     /locus_tag="PK049_06450"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOK61234.1"
                     /translation="MAKTILIVDDSDSIREVVRFTLENEGYNVLVGVDGKDALKHLDG
                     KTIDLVITDLHMPEMDGIELIKHIRAMPEYERIPILFLTTESQTSKKMEAKDAGATGW
                     IIKPFVPAKLISAINKVIR"
     gene            complement(8857..9165)
                     /locus_tag="PK049_06455"
     CDS             complement(8857..9165)
                     /locus_tag="PK049_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61235.1"
                     /translation="MSKQEKNFSIERQKGSSLVILSGDLSLNNISDIKSELIKLFNEA
                     KPTDLLIKNAEVIDLGFIQLLRSFNFSMKERGIKSTVKFELTADQSTILERSGIKSEY
                     "
     gene            complement(9179..10912)
                     /locus_tag="PK049_06460"
     CDS             complement(9179..10912)
                     /locus_tag="PK049_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61236.1"
                     /translation="MKKIKEISEQFAVIDSLIVEILNCAAEDFLGLNERFKEAYSKST
                     SISANAEEVFAVYASSYTSESLLNLRLLFKKFSQAKKETNKYADSIVKSIDEVYDILD
                     SIDLHSKNINQNLLTLKFLLANLKITGIESQSVELTEEKDELFIEFNRLVNKSKLAEL
                     ELAKSLHGNMKLLREGVDRVKKNMRNANQQIGIAIDIINESIQIFSEKQQDLSLNIPK
                     LQENNAKLRDSIDSIITNLQYHDIIRQKIEHVQVSHKEILNNLSSEDESEQKNYLNQI
                     GELVNIQSALLVRANKEYQRAIEQIIDKFKIIGSIAQSILNQCKDLKRIQESDLEQPF
                     TSVAHKLTNTTLPVSKFLSLLARVNNLLQSIKHNVNLMVETSAIDDEANRFGELLLKR
                     IKQPELMLQSNLIQQIETVTFDIITSRNQIGTLYMHLESKITDIERLSELISENTKSQ
                     EWESIPTTLKEIASNLLECDDKVSVLLREKLQASNDLGEIIHRAVNQVKYYEVFELKI
                     LEVIKLLNHIYSTLTGKEESQFDRDDLEFMRRLYTMESEHRIHNMVIKGNAGFDDLSE
                     TDQDESNIEFF"
     gene            complement(10924..11301)
                     /locus_tag="PK049_06465"
     CDS             complement(10924..11301)
                     /locus_tag="PK049_06465"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOK61237.1"
                     /translation="MDFKGYVLVIDDSVTNQVLVEALLGEEGIRVVTVSNAADAYRLI
                     DKEPPSLILLDLLMPNVTGIDFLKTIKQSERVNTIPVFIVTAANTELYRNQCEVLGVQ
                     EFFPKPVDIPLLVKRVKEVLAGV"
     gene            11447..12145
                     /locus_tag="PK049_06470"
     CDS             11447..12145
                     /locus_tag="PK049_06470"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOK61238.1"
                     /translation="MADVITISFEKTFEKCKLPVNQLLTAEQQAIIMNSINIVNYNRK
                     DIIVKQYSASSHVYYIIKGIIKVSSEQRKGKNLVMKIDTTGSFVGLISLLGGDSYDFT
                     VTAVENCTLAQIPITTLKSVMLQNARFALEISQLISRHGLFLINRLAGLLYKQLPGRV
                     ADLILYFADDIFCNNEFEFPVNRTELAELCGTTKESLIRTLSEFNHDRIIELERNRIR
                     IISYDILKTLSRFG"
     gene            12166..12540
                     /locus_tag="PK049_06475"
     CDS             12166..12540
                     /locus_tag="PK049_06475"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOK61239.1"
                     /translation="MPKILIVDDIFVNRLLLKEVMKNLNVTCLEAQNGKEAIDIITKE
                     KVDLVFMDIEMPVMNGLETTKYVREKLPSPKKYTPIVALTAHNPANFFEDFRDAGFDF
                     LMTKPYSIEKIKQAIEKVLTPT"
     gene            12609..13802
                     /locus_tag="PK049_06480"
     CDS             12609..13802
                     /locus_tag="PK049_06480"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOK61240.1"
                     /translation="MDRSILAELIRKNKRLIIPNVGAFLHRDTVSNNQLSITFSPFLK
                     YNDGQLEELLISNYGLSKIEAADQIKKLSIEIIEEIKESGSYSIPGIGILINDSKGSI
                     NLTSEESSSQRKSTDHDDGKNIQTTNIDPPLEVDASTGDKNQTTNDLAVKEEKVQEEN
                     TITKHYTEVSPGVERVNTNALDSIIVEKTSTDNSNNSNQFEYNDLEQVNMKSSSKKKP
                     SSPLRVFVFIAVSLFVILTFAFILRELAFAPDEADWENIKNKPEKVEPIKLPTDYTSK
                     SDDFDKKFEATNPADEESETVTGNEQPSQTTEEVIETSLTQSTPVGNPSTIYSLVVGS
                     FAEKANAQKFVNDLKAKGFSAEVYSKPNGKHLAVIGRYSKKDEANQQKEKLKNQFQGI
                     WIISR"
     gene            13805..14512
                     /locus_tag="PK049_06485"
     CDS             13805..14512
                     /locus_tag="PK049_06485"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HOK61241.1"
                     /translation="MNRTKQCTYPHIGTVTYTYRTGSKRITLRVKKDSSIHVTIPYLV
                     RFSYAEDFVLSKTDWIIKKIQENAERIKPITEFKSKYHLVRLQAENSLSKCKVLKKGA
                     DVLLLYPSTADPTSYNIQSLTKKVITEVLRAEAKYYLPQKLKELATKHNFTYGKVSIR
                     NSKSRWGSCSGKNNISLSLRLMYLPEHLIDYVLLHELCHTKEKNHGPKFWQMLDSVCQ
                     GNSKMFSKELKKFPIPF"
     gene            complement(14542..14823)
                     /locus_tag="PK049_06490"
     CDS             complement(14542..14823)
                     /locus_tag="PK049_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61242.1"
                     /translation="MASVKDLKKDIKQMVKHLLDECYTQLTYSEPISKERILDIISDI
                     MVLEQETISKISKKTYKRGESTKVDYQKIANDFYDEVVELAERINSLDE"
     gene            complement(14937..15401)
                     /locus_tag="PK049_06495"
     CDS             complement(14937..15401)
                     /locus_tag="PK049_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005928220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOK61243.1"
                     /translation="MKSRTITQQAEIEQVINSVDVCHIGMVNLEGNPYVLPFNFGYEE
                     GVLYFHSGPEGKKIDIWKHNPSVCVAFSSDYHLRYQHEDVACSWSMKYRSVLIYGKVE
                     EITSLDEKRKCMNIIMKKYSGRDNFEYSMPAIKNVKVFRVIPERIEGKAYGY"
     gene            complement(15473..18289)
                     /locus_tag="PK049_06500"
     CDS             complement(15473..18289)
                     /locus_tag="PK049_06500"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HOK61244.1"
                     /translation="MSSYKGLTDSEVIQSRNLHGVNIITPPPQTPWWKLLLEKFEDPI
                     IKILIVAAIIAIAIGITNGHYLEGVGIIVAILLATVMAFINEYKASKEFELLNSVNDE
                     LPVKVIRNGGVTSVPKRDVVVGDIVIVDRGDEIPSDGVLLESVSLIVNESSLTGEPST
                     NKTSIPTDSFKTTYSPNQVLKGTTVTDGHGIYRTTAVGDSTEIGKTARAATVEVNNPT
                     PLTKQLQKLSEYISSAAFFIAILAFVILTVFDLINPANNYTLEQEYILAVTVIAIIIL
                     LAKYWVPSVNAILSWIKLSAKIHLPYPNNTRRQMLVLLASAILLVLLGLVIAVLLNID
                     IFNASVVLDSNLLSHLLSYFMVSMTLIVVAVPEGLSMSVTLSLAYSMRKMTAENNLVR
                     KLQATETMGAATVICTDKTGTLTQNRMQVIECDFLGDTSSNGLPFLVKLSLAVNSTAH
                     IDFSHEPYTIVGNPTDGALLLWLYQNGVDYRVLRDGFTTLAQHPFSSEKKYMLTLGQS
                     TDFVEKLLLVKGAPEKVLSMCDPQSVSSSIMEKLREFQVKALRTIAFAYSKSEVDSIT
                     DSDTPLGLTFLGVVGIADPLRDDVPTAISECHMAGIDVKMVTGDNQDTAIAIGKNAGL
                     LASNAEHASISGPDFEKLPDDEALKVLPGIKVMYRARPTDKLRMVSLLQRLHNVVAVT
                     GDGTNDAPALNQADVGLAMGSGTQVAKDASDIILLDDSFSSIVNATRWGRALYQNIQK
                     FLYFQLTINLVAVAIVLTGPFTGIDLPLTVTQMLWVNLIMDTFAALALATEPPHSEVM
                     KKKPRSPHEFILSSSIFRYLITTAVLFYLVLVGLLFYLKHNEGFSDRNLTGFFNVFVL
                     MQFWNLFNARGLFGGVGVFSFSSNKLFIAIAIIILLGQFAIVQFGGEAFRTVPLTGKE
                     WLIYIIATMPVLIFGYLAKKFIKI"
     gene            18401..19117
                     /locus_tag="PK049_06505"
     CDS             18401..19117
                     /locus_tag="PK049_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOK61245.1"
                     /translation="MGRAFEYRKARKLKRWGNMARTFTRLGKEITMAAKAGGPDPETN
                     PKLRLLIQNAKAENMPKENVERAIKRATDKDTKDYKEVVYEGYGPHGIAILVETATDN
                     PTRTVANVRSYFNKYGGSLGTTGMLDFIFERKCSFKVAKNPNISMEDLELELIDFGVE
                     EVFAEDETIMLFAEFTQFGPIQKFLEEKGLSIISFQFERIPNDTKKLTPEQQTEVEKL
                     IEKIEEDEDVTNVFHNMAIE"
     gene            complement(19165..20268)
                     /locus_tag="PK049_06510"
     CDS             complement(19165..20268)
                     /locus_tag="PK049_06510"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOK61246.1"
                     /translation="MEDKLIVIATESNSKALVLKSYLEANGVECFLRSVNVLQGAIAE
                     GVMVLIRESDAEKALKLLSAMHHDEEIKKADQQLRKILVPVDFSVPSHNAARYAVMLA
                     SKYKAQIKLLHVFNSPVVDMIPFTDVSSIQIDFDMNYHVLYNSAKVRLVKFHENILEF
                     ANQMGFGEVQIGYTLREGYAAYGIIEASKRYKPGIIVMGTKGEGYRSTELVGSVAAEV
                     SDETHIPLLVIPEKAVLKGVDEVKRVLYITNFDSSDNASIRKLLKIVSPFQVELHCIH
                     VTGKSETPGINALITSTREYLASVTSNVSVKCDIIVGKETDKSVLEYIKKNNINLVAL
                     TSVKRNLIYKLFNPSLAKRMIYQSDVPVLLFHA"
     assembly_gap    20346..20445
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<20446..21176)
                     /locus_tag="PK049_06515"
     CDS             complement(<20446..21176)
                     /locus_tag="PK049_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61247.1"
                     /translation="MKMKRFTLFALFLAGMVSFVACEKDDTKDLNKDEAQQQITNAEQ
                     ELLTKSGEIVATDGYKVQEYFATQLPSPFYVKSDVSKSVVPSRFQKAYELTRQNLLLE
                     NPEIEFHFDYFLIEFFNDVKGTWTWAPDGWTHESTPTDKVIVKFPYPATNATNNVTIT
                     YYDFTTTLVYGETVPTGIKVKIEKDGNQVFSMVYSATIANMMKYSTKVTVTFGPFEIS
                     NEESFDASVNNKVLYSKNFTFKKDG"
     gene            21394..23130
                     /locus_tag="PK049_06520"
     CDS             21394..23130
                     /locus_tag="PK049_06520"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOK61248.1"
                     /translation="MKQFLSYILLSLNVIAWADANACTNYLVTRGASADGSTMVSYAA
                     DSHIRYGELYFKPRGIWPAGTMITLYDRGINKPLGQIPQAPETYQVIGFMNEYQVAIG
                     ETTFGGIEELMDSTAIVDYGSLMFLALQRAKTAREAIKVMVELVDQYGYASSGESFSI
                     GDPNEVWILEMIAKKTNLKYDKKTKQNVNIDKGAVWVAIRIPDGYISAHANQARIQQF
                     PLENHKTSISSKNLNLIFNPEVETVYSHDVISYARNHGYFNGSDSEFSFSDVYNPITF
                     DGARYCDARVWAFFNHASDGMGKYWDYAKGENLKNRMPLYVKPNRKLTPRDLMTFKRD
                     HLEGTELDMSKDAGAGPHGLPYRWRPLTWNVDGVTYFHERVTATQQTGFSFIAQMRSW
                     LPNPIGGIFWFGVDDAASTVYVPIYCGISRVPESYSEGNGDMLTYSPTSAFWVFNRVA
                     HFAYLRYDLIMKDVKKLQDELENKFEAYTPAIDAAALKLWENNTNLAIEFLTDYSVNT
                     ANSTVQKWNDLSNWLLVKYIDGNVKKEIDGKFLRNPWGYPVSPAQPGYREEWLRTIIN
                     ETGNKFKVPNQN"
     gene            23154..>23701
                     /locus_tag="PK049_06525"
     CDS             23154..>23701
                     /locus_tag="PK049_06525"
                     /inference="COORDINATES: protein motif:HMM:NF025111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spi family protease inhibitor"
                     /protein_id="HOK61249.1"
                     /translation="MKKFYTLLLLALSGLLVIANPVDVKLAKKVAINYLSAKKGASID
                     TFDLKLVNTHQYEGKDALYIFAMSKGGFIIVSSDDEAKPIIGWSLTNPMPKKIDNPVV
                     LERFNWYAKQVNYAAKSNIGDKSVKQEWQDILDGKIAKGIKGGGPLLATTWDQSPYYN
                     MLCPTGTPTGCVATAMSQIMRY"
BASE COUNT         7022 a         4881 c         4533 g         7065 t
ORIGIN      
        1 attttttctg tagcctttgc tttccacact tatcgaaaag aaataagcat ctcctggatt
       61 aaagagatgc ttaccaaata aagaagttat tgataatttt agaataaatc cactgagata
      121 ttctgaataa atgcagtata ggtgatttcc ctaccaacct tagcttctgt tataagcata
      181 agtactggta gttctttacc gcttttagtg agaatggtta tttccttgcg ttgtccagta
      241 attttttcct tattggggtc aaggtaggca tcaaggaatt catcattttc ggctgcatta
      301 tcggggaaaa gaattcttac actttgtcca atcacctgtt cgcgtttaac ctcaaaaagc
      361 tcttcggctg ccttgttgaa gaactgaaca ataccatcgt ggtcggtggt aatgatggcg
      421 tccagaaaac cctccagggt tttctcgttc cgtattttta ccttctcaat ttctttaaat
      481 tggttgcgca cctcctcctt agcctcccgt agctttttat taagctcaac ttcattctgg
      541 cgcaattgct cttcctgttt ctttagctgt tccgtttgac gctgagtttc gaactccatg
      601 tttttctcac gggtaatatc gttagctatt aggataacct ttgccacctc attgtacata
      661 tcgttaacag cagtaaaggt acaacggaac caacgctcat cgccattgct agtaagcatt
      721 cggaattgtc cctcatatgg aattccatgt attacatcgt cccatacgcg ctcaatattc
      781 ttacgttccg ctgaaggtac aaactcaaaa attgattttt ccttagcagc attaaggtta
      841 tatgaaaggg tgagaaggaa cttctcatta gcttcggtta agtcgccagt aggggagaat
      901 tcagctttaa ttgatgaacg gttaagagca ctaatttgcc cttcgtagtc aaggctcagt
      961 ttcttttggt cagttgtgtc aattccaagg aataggatct tatcaacacc tccggcatca
     1021 tttcgcacac aagtgaatgt tgcaattgtc cagaggtcat taccattgcg ggtaagtaat
     1081 ttcatatccc cttcaaagtg tttaccgccc ttactcagag tactccagat atcgtcgaac
     1141 cacagcctat cctttttatt gataaacatc gatatgtgtt tcccttccac ctcgctattg
     1201 gttgaatact ccaatttctg taaaaacttg gtgtttgcat acagcaatgt accgtccaca
     1261 tcgaaatcgg cacggatcat ggtgtggttc acggagttgg agaagctaat aaactttgct
     1321 gactgacggc ttgcctcttc ctgtgtggct tggagttctt ccatgttttg gcgcatttgt
     1381 tcctccttaa gcgccaactc ctcggcttgc tcgcgcgatt cctttaaaag ctttgcagtt
     1441 cgaatattta ttttaaccgt agcaatagtt gttgccacgc tctcagcaac cttatcgata
     1501 aattcctcct gataattttc aaaagggtta aacgatgcca attcaattac accatgaacc
     1561 acatcgttca tcttgagagg aataagcata agaaacttgg gatttgcttt accaagtccg
     1621 ctggtaatac tcaaataacc ttggggaatt ttcttaagta ttattttctc tttttccaaa
     1681 gcacatgctc ccactaaccc atcgccccaa ggtataattc tgtcggcata ctttctacgc
     1741 tcataggcgt agcaagctgt catgcggaaa tggatatcgg tttcatcgtc atcttccaaa
     1801 aggtagaatg ctgcttgatt tgcaccaacg taattcacta aattgcgaat aacctgaaag
     1861 gttaactcct ctatgttatc gttgttctga cgtaggattt caccaaagag agccataccc
     1921 tctgcagccc agtgccgttg ctcgtcctcc ttttttcttt tctcctcctc catcctgctt
     1981 tggcgaatat cgtttcgtaa gctgactatt gccttcccaa gtgcatcggt atcatcgggg
     2041 gtgtattcgg cttcaacatc accttcacgt aacttttcca caaacctgta gagcttacgc
     2101 tccctactta tgttttcctg taatgtggtt tcgaaatcgt ttattcggag cgaaagcttc
     2161 tggtaaacaa aaaatccaac ggcaccaaga atgataggta aaaaatcaaa tatatagaac
     2221 aatggcatga tgcgatgtag gtcctgaatg cctcccggag ttaatgcaag gtcattggtt
     2281 aatacaccta caacatatgc aatggcaaga aacacaagcc ctacggtaag tccaaggaga
     2341 ctcccttgaa caatgtattt caatgagtac ttagatttca tgtacgactc aatttttgat
     2401 gcttaaaaat aagcattttt tcaacttata tgcaaatcta ttgattcgaa ttcaaatgaa
     2461 ataataatgt tattataaaa gaaccggttt atcagcttaa tcgtgttttt actctttgga
     2521 taatatgatt cgcaatatcc tgtaaaggca gtatggtaga tgctgcatca agttttacgg
     2581 cctcacgggg catgccgtaa accacgcacg atttttcgtc ctgtgctatt gtgtaagcac
     2641 cagcctcctt catttcgagc agcccttttg ctccgtcgtc gcccatgccn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc gctttaaaag catatggtaa tttccggggg
     2821 ctattaacgc ttgacccggg agaagagcat cgccattctg tgcttcctta accgtaatct
     2881 tacatatatc gtttaagcga ttagcaaacg aggttgtaaa tttctcaggc atatgctgaa
     2941 ctataacaat tccaggacaa tcgacaggca ttgcttgtaa gaatgtagca atagcctctg
     3001 ttcctcctgt agaggctcct actgctataa ttttctcggt agttgtaagg ggtgttgagg
     3061 aaacaacctg tttagggatt ataacatcgg ccgagagttt tggctcaaca ataggaggtg
     3121 taagaatttt acgccttgct attctactgc gagcggctgc tttaattgca tcgcaaatga
     3181 taacctttga ctcttcgaga aaagctttag tgttaacctg gggtttagtg atcaattcaa
     3241 cggctccaag ttcaagggca cgcataccca tttcagttgc cctgcttgtg aggctcgaaa
     3301 ttattacaac cggtatgggg tgttgtgtca ttattttacg aagaaaggtt agcccatcca
     3361 tccggggcat ttcaatatcg agggttatta catcgggggt cacctcagcc atcttacggg
     3421 ctgcaaagta tgggtcggca gcagttccaa taacctccag ctcggaatcg gatgagatga
     3481 tctccaataa cgtttgcctc actaatgcag aatcgtcaac aattagcact tttattctat
     3541 tcagcatatc agccatttac atcaacttta aatttaagga aaataaaaat atctgcaatt
     3601 taatcggcct tttttgtact tatactttta acctcattgg agtttagtgt cttttgaata
     3661 aacttatgtc gaacctctcc tgtagcggta ttgtattcaa tctttcttcc ctggttacca
     3721 cctacacttt tagctattat tggtattcca tgttcagcca ataattctaa agcaatagca
     3781 atgttacgtt gaccgatatg gaagttgtcc agatttgttt caagcacctc gcctccccca
     3841 aataccttgg ccttaaggtt ttcctttttt gagccaagtg aaatcattct ttcaatcaat
     3901 cgttcaatgg caatgttacc ataccttggg cttgcaagcc cctgtccatt ccacaatggc
     3961 aacatgtaat gattaattcc accttgattt aataccgaat cgtataggca tactgccaca
     4021 catgagccca gaattgtagt aacaacatga ggctccttat cggcaaatag cgatgcaggg
     4081 tataggtaat gtgacttaaa gtggtccatc aaccggcttt aaaaaatttc tgcatcctga
     4141 tcaaaaaaca tctcgttagc atcggtggct aaatcgtcaa cgtcaacatt gggctcgggt
     4201 attgaaattg tgaagcaagc gcctttgcca agttcgctct caatttcaat gctaccattg
     4261 agcatatcaa taatagccct atttaccgat agtcctaaac catgccctcg gttcaatgaa
     4321 tttattcctg tgtcgagcct tttaaaccta tcaaaaataa ttttgtggtt ttcctgagag
     4381 ataccaatgc catgatccct aacacaaatc gttagcttgt taacgcctct actggtaatt
     4441 atttcaatta tgccaccctc aaagctaaag tttactgcat tgctgagcaa atttgctatg
     4501 atcagcttca gcttttcagg gtcggtttta aaaatttttg gattaaccaa attggtgctg
     4561 gtgttgtagg agtattcaac ctttaagttc ttcttgcgta gctcatactt aaacgattct
     4621 ataacacttt caagcaggtt gttaacatca acctgcatga tctcgggttg gatttcgcca
     4681 gcctcaatct tggcagctac aaagatatta cggagttgaa aatcgaggtt gaatgcctcg
     4741 gagtggatga gagccaccat ggagataacc tttttccagt tctccttatc aactgagagt
     4801 attgctttgg aaagtcctat tattgatgta aatgggttaa taatctcatt ggtgatgttt
     4861 gaaataaagt gcgatttgag ggcttcggac tcctctagct tcttgtttac ttgctttagt
     4921 tcggcgttta gaactttcag ttcatcaagc gcttttttat tgagttcaaa cctgcgctgc
     4981 aattcagcaa gtagttcatt atcggtaagt tcttcaggct ttttagtcat agcagaatgt
     5041 attatacttt cctgtatatt gttggtttaa cctgttttag cggaacatcc atatttagta
     5101 ttgattctga atgcccaagt attaggtagc cattgggttt aatacgttgg catagcttat
     5161 taataacggc ttcctgtgtg ttcctatcaa agtaaataag tacgttgcgg caaaaaacaa
     5221 tatcaaataa atcctttgta tcgtagctgg aatccatcag gtttaaacgt gcaaatgtta
     5281 ccttggagcg aacatgtggt gccattttga tggtagggtt agttctatcc ttgctcctta
     5341 gcatgtattt ccgcttaagg gttaacggta taccctcaac cttctcctcc ttatatactg
     5401 catctatcgc cgtttgaaga attcgggttg aaatatcggt tgcatatatg gtaaagttaa
     5461 aaccagggtg cgactcagcg aattcaaaaa gcaccatggc aagggtgtaa ggttcctcgc
     5521 cacttgaaca acctgcgctc caaaccttaa gcgtgcctga cttattggct ttataatatt
     5581 caggcagtaa ttcttcggtc aagaactcaa aatgggctgg ctcacggaaa aaatcagttt
     5641 tgttggtgct aactacatcc aacatatgga taatctcgct atcaaagcct tcgcggctga
     5701 aaaggtaatc gatgtaagcc ttaaaagagc caatgtttaa atgctttaac ctcttttgga
     5761 ggcgacattg gagcattacc ttttttatag gtggtagttt tatgccactc tccttgtaga
     5821 ttaatgcgct aagacgttta aactcatcgt cggtaagctg tagcttgtag taatccacaa
     5881 actcattagc ctgcataaaa tattcaatta ctgttgtgca tcggttccct cggcaactga
     5941 ctggttggaa tcaacaatcg aggtgatttc atctactgag aagatggcat ccatgttaag
     6001 tatgatgata aaacgttcct caacctttgc catgccaatt ataaactcgg atttataacg
     6061 gctgccaagg gtgggcaatg gcattatctg gctattatca atctccagaa ccgcctgcac
     6121 ggaatcgaca atggcaccaa catgcaccga ttcgccatca acgtcaatat ccagaactat
     6181 aatacaggtg ttattggtgt actcggtttc aggcattcca aacttgatac gtgtatcaat
     6241 tacaggcaat acagcccccc taaggttaat aacacctttc aggtaatcag gtgccttagg
     6301 aacctttgtt attggagtca tctccaaaat attcaacacc ttgctgacat gtgcagcaaa
     6361 ctcctcgtta cctagcttga aggtaagata tgatttaatt tgatttttgt tctcggtatc
     6421 cattgtaaaa catttttggt tgttacttac ttgttgcaaa cattttgata atcttattgg
     6481 tgtccattac aagggcaact gttccgtcgc ctagtattgt cgcacctgag aaaatatcgt
     6541 ggctcttgta gtgtttccct aaaggtttga gtactgcctg gtattcacca attacatagt
     6601 caaccactag gccaaccctg ttatcctcaa agtttactac tacaacctgt tctagttcct
     6661 cgctacttgg ctgcaggtta aactccttcc gtaagctaaa gaatgggatt tgagttccat
     6721 cgagggttac aagattgttg aaagagctat agatgctttc cttgtccacc gcataaatct
     6781 tgtcgattga agaaagaggg ataacaaaag gagagctatc aatgctaacc agcaagccat
     6841 cgataattga aagggttaag ggcagcttta tggtaatagt tgttcccttg ccaacttccg
     6901 attcaacctc aacctcgcct ctgatatcgg aaattttctt tttaaccaca tccataccca
     6961 ctcccctgcc cgacacatcg gttacctttt tggccgtaga gaatccgggc aaaaagataa
     7021 ggtcaagaat ctccttcttg ctcatctttt tatcggaagg tactaaaccc tttgagattg
     7081 ccttggcatg tatgatctca ggatcaatac cagcaccatc atcgctaacc tggattatta
     7141 cacttgcccc ggagtagaat gctttcaggt gaatttttcc ctttgccggc tttccgtttg
     7201 taatacgaac ccgagcatcc tcaattccat gatccatgct gttcctgata atgtgcatca
     7261 gggggtcgga caggttttcg atgatggtct tatcaagctc agtatcggct ccctcaacaa
     7321 tgaaatcgac ctccttgtgc agttcagccg ataaatccct gacaagcctc tgaaaacgac
     7381 caaaaaggtt ttcaatgggt atcagaacga tactaaaggc aatatcgcga agctggcggg
     7441 ttagcttgtg aacgttttca actatgggcg ccaaacctgg cacattggtt tgctcggcaa
     7501 aaaggcttaa acgggcttgt gtggttacca gctctgacac aaggttcatt aggtggtcaa
     7561 gcttgtcgga cgacacgcga atgcttgcag ccgttttgtc ctttgtggct attcgctctt
     7621 tagtgaactg tttggccttt tccttattga ttcgagcagc agcttttgtt gccaggcgtt
     7681 gtatggttac caatccaaca tccttctgaa cctctgccaa ttgcgatact tcttcaacaa
     7741 ataatttatc ggcaagcaag ttttcctctg ccaacttttg tatttcaagc tttgaatcgg
     7801 attcaacaaa gatgaacaca tcatgaatgg catttacatc ctggtcggtt gtcagcaaca
     7861 cttcccagta ggtatagcaa aggtttgggg tgatttcctt tattccaggc agcctgttga
     7921 aatgggcaaa aaccttgtgc tcacctaatg agcaaagctc atcaagaagg taaagggggt
     7981 ttgttccgtt tttaaaaata ttctcattgg gttcaaaaag aatgtaatag gtagatagtg
     8041 attccgattt ttcgttactt gatttagcag attcggcttc tgtttcagca ttactggggt
     8101 taattattct attaattttc cccattaagc cattgtgaac cgttttaaag tcgggatcct
     8161 catagttttg ctcatcgagc atcgccttga ggtgatccac cgatgaaaag gttatgttca
     8221 atatatcctt gcttacctcg agctgtccat tgcgaatcat gtcgtaaatg gtctcaaggt
     8281 tgtgcgtaaa gctatcgata aggtcaaacc caaacatggc gctgttaccc ttaagggtgt
     8341 gcatcaccct aaagacctgt tgaatcagag cctcatcgtt agggcttttt tcaagttcta
     8401 aaagcgacac ttcaagctta tcaattagct ccgcagcttc ctctataaac tttttcctaa
     8461 agctatccat tacctaatca ctttattgat tgccgaaatc agtttagctg gaacaaacgg
     8521 tttaataatc caccccgtag caccggcatc tttagcttcc attttttttg aggtttgcga
     8581 ttcggtagtc aggaatagga ttggaatgcg ttcgtactcc ggcattgccc taatatgctt
     8641 aataagctct atgccatcca tttcaggcat atgaaggtcg gtgatcacca aatcgattgt
     8701 tttaccgtca aggtgtttta aggcatcttt gccatcaaca ccaacaagca cattgtaccc
     8761 ttcgttctca agggtaaatc gaacaacttc gcgaatacta tcggaatcgt ctacaattag
     8821 tattgttttt gccatagcaa tggatgattt attttgttaa tattctgatt ttatgcctga
     8881 tcgttcaaga attgtgctct ggtcagcagt taattcaaac ttaactgtcg attttatacc
     8941 tcgctctttc atggagaagt taaaagagcg taagagttgt ataaagccaa ggtcaattac
     9001 ctcagcgttt ttaattagca agtcagtagg tttagcctca ttaaatagct taatcagttc
     9061 actcttgata tcactgatat tattaaggct taaatcgcca ctcagaatga ccagcgatga
     9121 acccttctgc ctttcgatgg aaaaattttt ttcttgcttt gacatactct tggtttgttt
     9181 aaaagaactc aatgtttgac tcatcctgat cggtttctga taagtcatcg aaacccgcat
     9241 tccctttaat taccatgttg tgtatcctat gttccgattc catggtataa agtcttcgca
     9301 taaactccaa atcatctcta tcgaactggc tctcttcctt gccagttaac gtggaatata
     9361 tgtggtttag tagttttatt acttcaagaa ttttaagctc aaaaacttca tagtacttaa
     9421 cctgatttac agccctatgg attatttcac ctaaatcatt actagcctga agcttttccc
     9481 ggagtaaaac gcttacctta tcgtcacatt caagcaggtt gctagcaatt tccttaaggg
     9541 ttgttggaat tgactcccat tcctgcgatt tagtattttc tgaaattaac tccgataatc
     9601 gctcaatatc agttattttg gattcaaggt gcatgtacag cgtaccaatt tggtttcggg
     9661 aggtaattat gtcaaatgtt actgtttcaa tctgttgaat tagattcgac tgaagcatta
     9721 gttccggctg tttgattctt ttcaacaaca attcaccaaa tcggttagcc tcatcatcaa
     9781 ttgcagaggt ttcaaccatt aagttaacgt tatgcttaat tgactgaagt aaattgttta
     9841 cccttgccag taacgaaaga aatttgctaa caggcaaggt agtattggta agcttatgcg
     9901 ctacacttgt aaaaggttgc tccaaatccg attcctgtat tctttttaaa tccttgcact
     9961 ggtttagtat gctttgcgca atgcttccaa taatcttgaa cttgtcaatt atctgctcaa
    10021 tggctctttg atactccttg tttgccctaa caagcagcgc cgactgaatg ttaacaagtt
    10081 ctcctatctg atttaaatag tttttctgct cactttcatc ttcagaggat agattattta
    10141 gtatttcctt atgcgatacc tgtacgtgtt caattttttg gcgaataata tcgtggtact
    10201 ggaggtttgt aataatgctg tcaattgaat cgcggagttt cgcgttattt tcttgcagtt
    10261 ttggtatatt gaggctaagg tcttgctgtt tttccgaaaa aatttgaatg gattcattaa
    10321 taatatcaat ggcaatccct atttgttggt tggcatttcg catgttcttt ttaaccctat
    10381 caacaccttc gcgcaaaagt ttcatgttgc catggagcga cttagcaagc tcaagttcag
    10441 ctagctttga cttgtttact aacctgttaa actcaataaa aagttcatcc ttttcctcgg
    10501 taagttcaac tgattgggac tcaatgcctg ttatcttaag gttggctagt aaaaacttga
    10561 gggttaggag attttggtta atgtttttgc tatgtaggtc aatggaatcg agaatatcat
    10621 aaacctcatc aatgcttttt acaatggaat cggcgtattt attggtttct tttttagcct
    10681 gactgaactt tttaaaaagg agcctgaggt tcagcaacga ttcactagta tagctggaag
    10741 catatacggc aaacacctct tcagcatttg ctgaaattga ggttgacttt gagtatgctt
    10801 ccttgaacct ttcgttaaga cctaaaaaat cctcagcggc gcaatttagt atctcaacta
    10861 taagactatc aattacggca aactgttcag atatctcctt gatttttttc ataacaattt
    10921 atattacacc ccagccaaaa cctctttcac cctcttaacc agtaatggta tatcaactgg
    10981 tttgggaaag aattcctgta ctcctagcac ttcgcattga ttacggtaaa gctcagtatt
    11041 agcagcggtt actatgaaaa ctggaatggt atttacgcgc tcactctgct tgatggtttt
    11101 aagaaaatct attccggtaa cattaggcat aagtaaatcc aagagaatta ggcttggtgg
    11161 ttctttatca atcagcctat aggcgtcagc ggcattgctt actgttacca ccctaattcc
    11221 ctcttcgcca agcagagctt ccacaagtac ctgattcgta accgaatcgt caattactag
    11281 aacatagcct ttaaaatcca tagtatttca aaatcttttc aaatctataa aaacgaaacc
    11341 acagaataac aaatcatatg atgatataaa ttaccgcaac aggtgattta tatcactttt
    11401 atatttgata gaaattattt atctttaaga gtctttaaat gaaatcatgg ccgatgtaat
    11461 aaccatatcg tttgaaaaga catttgaaaa atgcaaattg cctgttaacc agcttctaac
    11521 agcagaacag caagctatca ttatgaatag tatcaatatt gtaaactaca accggaagga
    11581 tataattgtt aaacaatact cagcatcaag ccatgtttac tatattatta aaggtattat
    11641 aaaggtaagt agcgaacaaa ggaagggcaa gaatttggtc atgaagattg acaccaccgg
    11701 aagttttgta ggcctaattt cgcttcttgg tggtgattca tatgatttta cggtaacagc
    11761 ggttgaaaat tgcacattgg ctcaaatccc tattactacg ctgaaatcgg ttatgcttca
    11821 aaatgcacgc tttgccctag aaattagcca gctaataagc aggcatggtt tattccttat
    11881 aaacaggcta gccggattac tttacaagca gctacccggc cgagtagccg atttgattct
    11941 ttactttgcc gatgacatat tctgcaacaa cgagtttgaa tttcccgtaa acagaacaga
    12001 gctagcagag ctgtgtggaa caactaaaga aagtttgatt agaaccctat cggaatttaa
    12061 ccacgataga ataattgagt tggaacggaa tagaatcaga attattagtt acgatatatt
    12121 aaaaacattg agcagattcg gttaacgtaa taaaagtaaa aatatatgcc aaaaattctt
    12181 attgtcgatg acatttttgt taacaggctt cttctaaaag aagtgatgaa aaacttaaat
    12241 gtaacttgcc ttgaggcgca aaacggtaag gaagctattg acattattac taaggagaaa
    12301 gttgacctgg ttttcatgga cattgaaatg cctgtgatga atgggctgga aaccaccaag
    12361 tacgttcgtg agaaactacc ttcacccaaa aagtacaccc ccattgtagc gttaaccgca
    12421 cataatccag caaacttttt tgaggatttc agagatgcag gttttgattt tctgatgact
    12481 aaaccatact ctattgaaaa aattaaacag gctattgaaa aggttcttac tcccacataa
    12541 aaagtacaac taataggata ataatttacc tttgctggta ttgagctaac cataaaacat
    12601 ctcgcacgat ggacaggtca attctggcag agctaattcg caaaaacaaa cgactaataa
    12661 tacccaacgt gggtgccttc cttcatcgtg atactgtaag caacaaccag cttagcatta
    12721 cttttagccc attcctaaag tataacgatg ggcaactcga agaattactg ataagtaatt
    12781 atggactttc aaagattgag gcagccgacc aaataaaaaa gctatcgata gaaattattg
    12841 aagaaataaa agaaagtggt tcatactcaa tacccggtat cggaatacta ataaatgatt
    12901 caaagggttc cattaacttg acatcggagg aatcatcaag ccaaagaaag tcaaccgacc
    12961 atgacgatgg taaaaatatt caaaccacca acatcgatcc ccccctcgag gtagacgcga
    13021 gtaccggtga caaaaaccaa accaccaatg atttagctgt aaaagaagag aaagttcaag
    13081 aagagaacac tattacaaaa cattacactg aggttagccc tggggttgag cgtgttaaca
    13141 ccaatgccct cgacagcata atagttgaaa agacgtcaac ggataatagc aataatagca
    13201 atcaatttga atacaatgat ttggaacaag tcaacatgaa aagttctagt aaaaaaaagc
    13261 catcatcgcc cctgcgagtt tttgttttta tagcagtgtc gctgtttgta atacttacat
    13321 ttgcattcat tttaagggag ttggcctttg cccctgatga agccgactgg gaaaacatta
    13381 aaaacaaacc ggaaaaggta gaacctatta agttaccaac cgattatact tcaaaatccg
    13441 atgattttga taagaaattt gaagccacaa accctgccga cgaggagagc gaaaccgtta
    13501 caggtaatga gcaaccatca caaacaacgg aagaagtaat tgaaacatca ttaacccaaa
    13561 gtactccagt tggcaaccca tcaacaattt acagccttgt ggttggaagt tttgccgaaa
    13621 aggcaaatgc ccaaaaattc gtaaacgatc ttaaggcaaa ggggtttagc gctgaggttt
    13681 atagcaaacc taacggaaag catcttgctg taattggccg atatagtaaa aaagacgaag
    13741 ccaatcaaca gaaggaaaag ctcaagaacc aatttcaggg aatatggatt attagccgat
    13801 agcgatgaac agaactaagc aatgcaccta cccacacatt ggaactgtaa cctacacgta
    13861 tagaaccgga agtaaaagaa tcactttaag agtaaaaaaa gatagttcaa tccatgttac
    13921 catcccctac cttgtaagat tctcatatgc agaggatttt gtgctatcaa aaactgactg
    13981 gattataaag aaaatccaag aaaatgcgga gcgtataaag cctattactg agtttaaaag
    14041 taagtatcac ttagttcgcc tgcaagccga aaatagccta tcaaaatgta aggttttaaa
    14101 aaaaggagct gatgtgctgt tgctttatcc gtcaaccgct gatccgacaa gttacaatat
    14161 acagtcgcta acaaaaaaag ttataaccga agtattacgc gccgaggcta aatactatct
    14221 gccccaaaag ctaaaggaat tagccactaa gcataatttt acctatggta aggtttccat
    14281 tcgtaactca aaatcgcgat ggggaagctg tagcgggaag aacaatatta gccttagcct
    14341 aaggctaatg tatttgccgg agcatcttat tgactatgta ctcctccatg aactttgtca
    14401 cacgaaggaa aagaatcatg gccctaaatt ttggcaaatg ttggactcgg tttgccaagg
    14461 aaattcaaaa atgttctcaa aagagcttaa gaaatttcca atccctttct agctttaacc
    14521 taataaccca caaacaggga attactcatc caaggagtta atgcgttcag ccagttcaac
    14581 cacttcgtcg taaaaatcat tggctatttt ctggtaatca actttggttg attcgcccct
    14641 tttgtatgtt ttttttgaga tttttgaaat ggtttcctgt tctaaaacca ttatatcgga
    14701 aatgatgtca agaatccgct ccttagatat tggctcagaa taagttaatt gggtatagca
    14761 ctcgtcaagc aaatgtttta ccatttgctt gatgtccttt ttaagatctt ttacgctggc
    14821 catatcaaaa tattaactac aatttttcca tataaatata aaccatttct taaaaaacta
    14881 gtaataattg atattttttt tgatgaaaag tgttttcaaa attttactgg atttcactag
    14941 tatccgtaag ccttaccttc aatcctctca gggataactc taaaaacttt tacattttta
    15001 atggctggca tactatactc aaaattatcg cggccagaat attttttcat tataatattc
    15061 atacatttcc tcttttcatc taaactggtt atttcctcaa ccttcccgta gataagaacg
    15121 ctacggtact tcattgacca tgagcaagca acatcctcat gctggtagcg taaatggtag
    15181 tcggaactaa aagctacaca tacgcttggg ttatgtttcc aaatatctat tttttttcct
    15241 tcggggccag aatgaaagta aagcacacct tcttcatacc caaagttaaa gggtaaaacg
    15301 taagggtttc cctccaaatt gaccatacca atgtggcaaa catcaaccga attgataacc
    15361 tgttcaattt ctgcttgttg agtaatagtc cgagatttca ttgcaattac ttttattgtt
    15421 taatacaaat taagtaaaaa atgatttcgc acgctactca ctatgtaccg ttttaaattt
    15481 ttatgaactt ttttgctagg taaccaaaaa tcagcacagg catggtagca ataatataaa
    15541 ttagccattc cttaccggtt aatggaactg ttctaaaagc ttcgccacca aactgtacta
    15601 ttgcaaattg tcctaacaga attattatgg caatagcaat aaatagttta ttggacgaaa
    15661 agctaaacac accaacacca ccaaacaacc ctcgtgcgtt aaaaaggttc cagaactgca
    15721 ttaaaacaaa tacattaaag aagcctgtga gattcctatc actaaaacct tcattatgct
    15781 ttaagtaaaa tagtaagcca accagaacta agtaaaataa aactgctgta gttattagat
    15841 acctaaaaat actagaactc aaaataaact catgaggtga acggggtttc tttttcataa
    15901 cctcagaatg tggtggttca gtggctagtg caagagctgc aaaggtgtcc ataatcaggt
    15961 taacccacag catctgggta acagttaagg gtaaatcgat cccagtaaat ggcccagtta
    16021 gcacaattgc aacagcaaca agatttatgg taagctgaaa gtacaggaat ttctgaatgt
    16081 tttggtaaag agccctaccc cagcgggttg catttacaat gcttgagaac gaatcgtcca
    16141 aaaggataat atcggaggca tctttggcca cctgggttcc tgaacccatt gctaaaccca
    16201 catcggcttg gttaagggcc ggggcatcat tagtaccatc acctgtaacg gcaacaacat
    16261 tatgtaacct ttgaagtagc gataccatcc gcaacttgtc agtaggtcga gccctgtaca
    16321 tcacttttat accgggcaac acctttaaag cctcatcgtc gggaagtttt tcaaaatcgg
    16381 ggcccgaaat ggatgcatgt tcagcattgc tggccagtaa tcctgcattt ttaccaatag
    16441 caattgcagt atcctggtta tcgcctgtaa ccatcttaac atcgatacca gccatatggc
    16501 actcacttat tgcagtagga acatcgtcgc gcaatgggtc agcaatacca acaactccca
    16561 ggaatgttaa gcccagaggc gtatcagaat cggtaatgct atcaacttca ctcttggaat
    16621 aggcaaatgc aatagttcgc aatgccttta cctgaaattc ccttagtttt tccattatac
    16681 tactactaac cgattgggga tcgcacatgg acaaaacctt ttcgggtgcg cctttaacca
    16741 aaagtaactt ttccacaaag tcagtagatt gccccagggt taacatgtac tttttttccg
    16801 atgaaaaagg atgctgagct aaagttgtaa aaccatcgcg aagcacacga taatcgacac
    16861 cattctggta aagccaaagt aaaagggcac catcggtagg atttccaaca atggtatatg
    16921 gttcatgcga gaaatcgata tgagccgtag aatttacagc caacgataat tttacaagaa
    16981 acggaagtcc attgctactt gtatcaccca aaaaatcgca ctcaataacc tgcatacggt
    17041 tctgggtaag tgttccggtt ttatcggtac atattaccgt ggcagccccc atggtttcag
    17101 ttgcttgaag ttttcgtacc aggttgttct cagcggtcat tttgcgcatg ctgtaggcaa
    17161 ggcttagggt aacgctcatt gagagacctt caggaacagc aacaactatc agggtcatgg
    17221 ataccataaa atagctaagc aggtgactta atagattact atcgagcacc accgatgcat
    17281 taaaaatatc gatgtttaat aaaaccgcta ttaccaatcc cagtaaaacc agtaaaattg
    17341 ccgatgctag tagcacaagc atctgtcgtc tcgtattatt ggggtaaggt agatgaattt
    17401 tagctgacaa ctttatccag ctaagaattg catttactga aggaacccag tacttggcta
    17461 gcaaaatgat aatggcaatt actgtaacag ccagaatata ctcctgctca agggtatagt
    17521 tgttagctgg atttatcaaa tcaaaaacag tgagtattac aaatgccaga atagctataa
    17581 agaatgctgc tgaactaatg tactcgctca gcttttgaag ctgcttggtt aaaggggttg
    17641 gattatttac ctctacggtg gcagctcggg ctgttttacc aatttcagta ctatcgccta
    17701 cggcagttgt acggtatatg ccatgaccat cggtaaccgt agtgccctta agaacctggt
    17761 tgggtgaata tgttgtttta aagctatcag ttggtattga ggttttgttt gtagatggct
    17821 ctccggtaag ggatgactcg ttcactatta gcgaaaccga ttccaaaaga accccatccg
    17881 agggaatttc atcaccacga tcaacaataa caatatcgcc gactaccaca tcgcgcttag
    17941 gaaccgatgt aacccctcca ttacgaataa ccttaacagg gagttcatcg ttcacactgt
    18001 taagcagttc aaactctttt gatgctttgt actcatttat aaatgccatg accgtagcca
    18061 atagaatagc cacaataatc ccaacgccct ccaagtaatg gccattagtt atgccaatag
    18121 caattgcaat aatggcggct acaatgagaa tcttaataat aggatcttca aacttttcaa
    18181 gtaatagttt ccaccatgga gtttgaggtg gcggggtaat gatattcaca ccatgcaaat
    18241 tcctgctttg aataacctca ctatcggtta aacccttgta actactcata tctgcaaaaa
    18301 ataagcaaca caaagaaaac aaaaagattt aagaatggtt ggtcgaatag ccaaaaaaat
    18361 atacgtttgc agttaaattg aactaacaac ttttcacaaa atgggaagag catttgaata
    18421 ccgaaaagca cgcaaactga agcgctgggg aaacatggct aggacattta ctcgtctggg
    18481 aaaagaaata accatggccg caaaagccgg cggacccgat cctgaaacta accctaaact
    18541 ccggctttta atccagaatg caaaggccga aaacatgcct aaggaaaatg ttgagcgtgc
    18601 cataaaaaga gcaaccgata aagacaccaa ggattacaag gaagttgttt acgagggtta
    18661 tggcccccat ggcatagcca ttttggttga aacggcaact gataacccta caagaactgt
    18721 ggctaatgtt cgtagctact ttaacaagta cggaggttcg cttggaacta ctggtatgct
    18781 cgatttcatc tttgaacgaa agtgctcatt caaggttgcc aaaaatccaa acatatcaat
    18841 ggaggattta gaacttgaac tcattgactt tggagttgag gaggtatttg ctgaggatga
    18901 aaccataatg ctatttgctg agttcaccca gtttgggcct atacagaaat ttctcgaaga
    18961 aaaaggatta agcattatta gcttccagtt tgagcgaatc cctaacgaca ccaaaaaact
    19021 cacgcccgaa cagcagacag aggttgaaaa actcattgaa aaaattgaag aagatgagga
    19081 cgtaaccaat gtgtttcaca atatggctat tgagtaaatg tccattacaa ttatcaaaat
    19141 ggccacccta aagtggccat ttttttatgc atgaaaaagg agtaccggta catccgattg
    19201 gtaaatcatc cgtttagcca acgatggatt aaagagttta taaatcagat tccttttaac
    19261 cgaagttaga gcaacgaggt taatattgtt cttttttatg tattcaagga cactcttatc
    19321 cgtttccttg ccaacaataa tatcgcactt tactgacaca ttagatgtta cgcttgccag
    19381 atattcacgg gtagaggtta taagggcatt tattcccggg gtttcggatt tacctgttac
    19441 atgaatgcag tgaagctcaa cctggaatgg tgaaacgatt ttaaggagtt tacgaataga
    19501 agcattatcg ctactatcaa aattggtaat gtaaagcacc cttttaacct cgtcaacacc
    19561 tttaagaaca gccttttcgg gtattacgag tagtggaata tgggtttcgt ccgaaacctc
    19621 ggcagccacg ctacctacaa gttcagtact cctatagcct tcacctttag tgcccataac
    19681 tattattccg ggcttatagc gcttactggc ctcaataatg ccgtaggcgg catatccctc
    19741 gcgtaaagtg tatcctattt gcacttcgcc aaaccccatc tgattggcaa attctaaaat
    19801 attttcatgg aattttacca agcgcacctt tgcggaatta taaagcacgt ggtagttcat
    19861 gtcaaaatca atttgaatgc ttgacacatc ggtaaaaggt atcatatcaa ccaccggtga
    19921 gttaaatacg tgcaatagct taatttgagc tttatactta gaggcaagca ttacagcata
    19981 acgagcggca ttatgcgaag gtactgaaaa atcgactggt acaaggattt tgcgcagctg
    20041 ttgatctgcc tttttaattt cctcatcgtg gtgcattgcc gatagtaatt tcaaagcctt
    20101 ttcagcatct gattcccgga ttagcaccat caccccctcg gctatggctc cctgcagtac
    20161 gttaacactc ctcaaaaagc actccacacc atttgcctcg aggtacgatt ttagtaccaa
    20221 agcttttgaa ttactttcag ttgcaatgac tattagttta tcttccatgg cattttagtt
    20281 tttaataaat ttaaacaact gaacaataaa agtcaagttt tttataaaag aaaaaaggct
    20341 gcaaannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnttgcc atccttctta
    20461 aaggtgaagt tcttactgta aagaacttta ttgtttactg atgcatcgaa tgattcttca
    20521 ttagaaattt caaatggccc aaaagtaacg gttaccttag tgctgtattt catcatgtta
    20581 gcaatggtag cagaataaac catgctaaac acctggttgc catccttttc aatcttaacc
    20641 ttaataccag ttggaacagt ttcaccgtaa accagggtgg ttgtaaagtc atagtaggta
    20701 atggttacgt tgttggtagc attagttgcc gggtaaggga atttaacaat aaccttgtcg
    20761 gttggagtag attcatgtgt ccacccatca ggagcccagg tccaggttcc tttaacatca
    20821 ttgaaaaact caattagaaa ataatcaaaa tggaattcaa tttctgggtt ctctagcagt
    20881 aggttctgac gagtcaactc gtaagccttt tgaaagcgcg agggtacaac gctttttgaa
    20941 acatctgatt ttacgtagaa tggcgaaggc aactgggttg caaaatattc ctgaaccttg
    21001 tacccatcgg tagcaacaat ctcaccactc ttggttagta actcttgctc agcattggta
    21061 atttgctgct gtgcttcatc cttgttcaaa tcttttgtat catccttttc gcaggcaaca
    21121 aaagatacca ttcctgccaa aaacagggca aataaagtga accttttcat tttcatagct
    21181 ttaaatttta tagttattaa attaaacata caagcaagtt aatgcaaatg gcatgccaaa
    21241 gcaatttttt cagctgtttt atgtcaatcg atacatgaat ttagtcaaca cttatggtga
    21301 aattgtcaac aaaacatgat tttaagaaat tgctccttag ttcataaata aatttttcta
    21361 tttttgttgg ttaacataaa ttaaggattg gagatgaaac agtttctttc atatatttta
    21421 ttaagtctga atgttatagc ttgggctgat gctaatgctt gtaccaacta cttggtaacc
    21481 cggggcgcaa gtgccgatgg ttcaaccatg gtaagctatg ctgccgattc ccatatcaga
    21541 tatggggagt tatactttaa accacgtggc atatggcctg caggtacaat gattaccctt
    21601 tacgataggg gaataaacaa gccccttggg caaatccctc aggcccccga aacctaccag
    21661 gttatagggt ttatgaatga gtatcaggtt gctataggtg aaactacttt tggaggaata
    21721 gaggagttga tggactcaac cgccattgtt gactatggta gtttaatgtt tcttgcactc
    21781 caaagggcaa aaactgctcg tgaagccatt aaggtgatgg ttgaacttgt agatcagtac
    21841 ggttatgcca gtagcggaga gtctttctca attggcgatc ccaatgaggt ttggatcctg
    21901 gaaatgattg ctaagaaaac caacctcaag tatgataaaa aaacaaagca aaacgtaaat
    21961 attgataagg gtgctgtttg ggtggccatt cgcatccccg acggttatat ttcagcacat
    22021 gccaaccagg caagaattca gcaattccct ctcgaaaacc acaaaacttc aattagtagc
    22081 aaaaacttga acttaatttt caacccagag gttgaaacgg tttactcaca cgatgttatt
    22141 agctatgccc gtaaccacgg ttactttaac ggttccgaca gtgaattcag tttcagcgat
    22201 gtgtataacc caatcacctt tgatggtgcc cgatattgcg atgcccgggt ttgggctttc
    22261 tttaaccatg ctagcgatgg aatgggtaag tattgggact acgccaaggg tgaaaacctc
    22321 aagaacagaa tgccccttta cgtaaaacct aaccggaaat taaccccacg tgatttgatg
    22381 acctttaaac gcgaccacct ggaaggcact gaacttgata tgagcaagga tgctggagca
    22441 ggtccccatg ggctacctta ccgctggcga ccactaacat ggaatgttga tggtgtaacc
    22501 tactttcatg agcgtgttac ggccactcaa cagacaggtt tttcgtttat tgcgcaaatg
    22561 cgcagctggc ttcccaaccc aattggtggt attttttggt ttggggttga cgatgccgct
    22621 tcaacagttt acgttccaat atattgcgga ataagcaggg ttcccgaaag ctactctgag
    22681 ggtaatggcg atatgctaac ctactccccc acttcggctt tctgggtgtt taaccgtgtt
    22741 gcacattttg catacctacg ctacgatttg ataatgaagg atgtaaaaaa attacaggac
    22801 gaactggaaa acaagtttga agcctataca cctgcaattg atgcagcagc tctaaagctt
    22861 tgggaaaaca atactaacct tgccattgaa tttttaaccg attattctgt aaacactgcc
    22921 aacagcacgg tgcaaaaatg gaacgactta agcaactggc tattagtgaa gtacattgat
    22981 ggcaatgtga agaaggaaat agatggtaaa ttcttacgaa acccatgggg ctatcctgtc
    23041 agtcccgcgc agcctggata ccgtgaggaa tggttaagaa caataataaa cgaaactggc
    23101 aataagttta aagtaccaaa tcaaaattaa cagtaactca aaaatcaaaa ccaatgaaaa
    23161 aattttacac actactgcta cttgcgctct caggattact tgttattgct aatcctgttg
    23221 acgtaaaatt ggccaaaaag gttgccatta actacctgag cgcaaaaaag ggggctagta
    23281 ttgatacctt cgatttaaag ctggtaaaca cacatcagta cgaaggtaaa gatgcccttt
    23341 acatttttgc tatgagtaaa gggggattca ttatagtatc gtccgatgat gaggcaaagc
    23401 ctattattgg atggtcactt accaatccta tgccaaaaaa gattgataac cctgtggttt
    23461 tagaacggtt taattggtat gccaagcagg taaactatgc agcaaaatca aatattgggg
    23521 ataagtcggt taagcaggaa tggcaggaca tacttgatgg gaaaattgct aaaggtatta
    23581 aaggtggcgg tccgctttta gcaaccacat gggatcaatc gccctactat aatatgcttt
    23641 gccccacagg aactcctact ggctgcgtag ccacggcaat gtcacaaata atgcgttacc
    23701 a
//
LOCUS       DAOSAL010000036        23533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_1557_length_23533_cov_51.354417, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000036 DAOSAL010000000
VERSION     DAOSAL010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 23533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23533
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1557_length_23533_cov_51.354417"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="PK049_06530"
     CDS             <1..720
                     /locus_tag="PK049_06530"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOK61250.1"
                     /translation="VPPGSSPEMFEPTPQQMAELTQAKGYFSIGLLDFEKGMEQKLKD
                     NAEFRYIDLSQGLELIKGECEHNHNHESHQGHNHAVDPHTWMSAANARVMAKSIAQSL
                     TALFPERESEFNANLNRLVAHIDSVDNEVRGILAGSGRKTFVIYHPALGYFSRDYGLT
                     QLSVEHEGKSPSAKGVMELVERCRAEGITTILSQSQFDTHNSQTIANELGGTVVKVDP
                     LQENWAESMIFIANAIAGNVK"
     gene            717..1499
                     /locus_tag="PK049_06535"
     CDS             717..1499
                     /locus_tag="PK049_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOK61251.1"
                     /translation="MTNQTDKLIELVNVTAGYGKNIVLRNVNLAINTNDFIGIIGPNG
                     GGKTTLIKLLIGQLKPFTGEVIYHTKGNGSLIGYLPQASQIDKNFPISVREVVLSGLL
                     EKKGIFGGYSRQQKGVALDLMKQVGIANLQNKSIGQLSGGQLQRALLCRALISAPQVL
                     ILDEPNTYVDSNFEGELYEMLRKLNERMAIVMVSHDLGTITSYVKTIACVNGTLFYHQ
                     SNQITNEQLAAYNCPIQIIAHGPVPHTVLLDHGKENQHNHNH"
     gene            1511..2347
                     /locus_tag="PK049_06540"
     CDS             1511..2347
                     /locus_tag="PK049_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOK61252.1"
                     /translation="MIEAVKQFFQYAFIQNAIISAFLLSVITGFIGSYIVVRRLVFLS
                     GGITHASFGGIGIAWYFGLNPILGATVFSVFSALGLEYLSQGRTLREDSAIGMLWSFG
                     MAVGILFVFLTPGYAPNLMSFLFGSILTISQTDILLLLILATLTLVFFVAFYYPILFV
                     AFDSDFAKTQNVPVKFISYSLAVLIALSIVFAIRAVGIILIISLLTIPANIANIVTHR
                     FRRIIVLSALISLFSIVAGIAISFRLNVPSGATIIITMMAFYHIAKGIQLVRQRIFTL
                     KV"
     gene            complement(2352..2798)
                     /locus_tag="PK049_06545"
     CDS             complement(2352..2798)
                     /locus_tag="PK049_06545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HOK61253.1"
                     /translation="MRDKISIALSSFQGSANCAQSVLLAFATDEGLTTEQCLKLGAGL
                     GGGIGHKQHVCGAINAGAMIIGLRFGNSNLNDSDSKQKATTLVAEYMDKCAKRLGSTD
                     CCRILGIDLNNPEAKQKARDAGLFDMVCGNAIKVVCQLLEEDYRKV"
     gene            3031..4419
                     /gene="lpdA"
                     /locus_tag="PK049_06550"
     CDS             3031..4419
                     /gene="lpdA"
                     /locus_tag="PK049_06550"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOK61254.1"
                     /translation="MNFDLIVIGSGPGGYVAAIRASQLGMKVAVVEKENLGGICLNWG
                     CIPTKALLKSAQVFEYASHAADYGVLAADVKPDFEKMIARSRGVADAMSKGIQFLFKK
                     NNISVINGFGKLKDNFTVTVTASDGTQADYTAKHIILATGARSRELPNLKQDGVKVIG
                     YRQALTLPKQPASMVVVGSGAIGSEFAYFYNAIGTKVTLVEYMPNIVPLEDEEVSKTL
                     ERAFKKAGITVKTEASVEAVDTSGELCKVTIQTKKGPEVVEAEVVLSAVGITPNLEGI
                     GIEELGIILDKGKVKVDEFYRTNVEGIYAIGDIVPGPALAHVASAEGIVCVEKIAGLN
                     PHPINYSNIPGCTYTTPEVSSVGMSEKAARDAGYEIKVGKFPFTASGKATAAGNRDGF
                     VKLIFDAKYGELLGAHMVGGNVTEMIAEMVVARNLETTGHELIKSIHPHPTMSEAIME
                     AAAAAYGEAIHI"
     gene            4939..6879
                     /gene="dnaG"
                     /locus_tag="PK049_06555"
     CDS             4939..6879
                     /gene="dnaG"
                     /locus_tag="PK049_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOK61255.1"
                     /translation="MIDQHTVDKILAAADIVEVVKEYVTLRKRGVNYLGLCPFHNEKT
                     PSFTVSQAKGIFKCFGCGKGGNVVNFIMEHERLNYVEALKFLAKKYHIEIEEKEVTPE
                     EIEKRNERESLMVINSYAQRYFSDMLHKHSDGKTIAMAYFRERGFKDHIIERFQLGYC
                     LDQRDAFTQSALRDGFKLDYLVKTGLTIKRDDGSTFDRFAGRVMFPIHSISGRVIGFG
                     GRILKTDKKTAKYLNSPESEVYHKSEVLYGIFHAKKAITQENKCYLVEGYTDVISLHQ
                     TGIENVVASSGTSLTQDQIKLIKRFTPNVTILYDGDAAGIKASLRGIDMILEEGLNVK
                     VVLMPEGEDPDSFARSHNASEVLEYINQNETDFIKFKTRLLLDDAKSDPIKRANLITD
                     IVRSISAIPDQVIRAVYIKECAKLMDIGEDVLYSEVGKLRRSRMEQLLQRERTEAVRE
                     HETPKIPAFVQGISCEEQEKEIVRFLLNSGNQVLFSQEVEETNELITITAAQYIIDEI
                     LNDDLEFQNLIYKKVFDEYVRLLETNVEVDNRFFINHTDPEISQMAVDLLTEKYVLSR
                     IWEKHKAEVTESPDFLQTAIPKAIMVYKSKVLKLAISKLTDELSKLKPDQVEETNQLL
                     LRLRELYALMNKMSKDLDRVIL"
     gene            7119..7931
                     /locus_tag="PK049_06560"
     CDS             7119..7931
                     /locus_tag="PK049_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61256.1"
                     /translation="MKNYTNTLRVLTLAMLATVFFSCGNKQAKQAEELQAESKEQAFQ
                     EVTSYPIPTSFEVIQMINKAGASFIIGICNPVDNVGKYMTEKAKAVNLGIYGADLAYS
                     TTYQMKQETMNFLKVCKQLVEELNINAGFTPELALNVEKNIDNKDSLIVIFTNSYYET
                     YRTLVQNQKESLSLLVVAGSWIEGVYITSQIALTSRDNTEFIKILANQKAPLGKLMEL
                     MTPRVDIPEVKELMDMLAPIQAIYNKVEGEKLTNDQFQKLMEEITKVRNALV"
     gene            8051..11125
                     /locus_tag="PK049_06565"
     CDS             8051..11125
                     /locus_tag="PK049_06565"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOK61257.1"
                     /translation="MDKNIAIGLIDTLADLTRKDWDIEQCRSIIQSFFSSPDFDVNGE
                     ELLKQFDSIISSESFVSGNLKHDAGLPCETMTTEQRIELAIAFYHVLSSYPESGHQKL
                     FTAWNVIGIQPEDAICLYHFLSFGPSANQFQLPGNSFISQDVLEGWEQNNSNRMIFLY
                     DENSPTQELDKALCEKRTGLGGNLLFAYFHSIGCYIVKYHGDRQVLIEDEPLIAGRSY
                     CFKPGSSITLSNGEQIRYDDITRIRLLGSGVQPVKLVVQDLEFTFPGSNQGIKPFSTV
                     EESGRVVGILGGSGVGKSTLLNLLSGKLKPHRGKVLINGYDVHNESHKLMGVVGFVPQ
                     DDLLIDNLSVYQNMLFNARLCFGNYSRKQVRELVDSTLKSLDLWEIRNLKVGNALDKV
                     ISGGQRKRLNIGLELLREPAVLFLDEPTSGLSSNDSVMVLNLLRNLADAGKLVVVNIH
                     QPSERLFRMFDRLWVLDKGGYPVYVGNPTEAVGYFREKSARVGNLVTSAVQRKRVNPE
                     DILDIIEQRQVDRSGSFTVNRKVSPEEWYRLYRESIQPKVIEIEGKTALPQGKFRLPD
                     VIKQLNVFTKRNLLSKLANRQYMLLNILEPVVLGLILSFFIKYSPGNDYIFAANRNIP
                     AFIFMSVIVSLFLGLSVSAEEIIGDRRILERESFLNLSRFSYINSKVLYLFGLSAIQM
                     LLFVGVSLTIIGVKGLTLKYWLVLFSSACFANMLGLIISSVMKSVVAIYITIPLLLVP
                     QILLSGTVVDFDNLNSSLTRRVYVPVIGDVMTSRWAYEALAVTQYVDNRYEKNFFDAE
                     MQLSRAAFRASFLIPRLQVKLEECVRLDDLDEDRSADISRHLRFIANEIEHLAQSDDV
                     PQFELLVDLKKGQLYDQLAFELNGYLLYIKKLFQEQQRAATAKRDSVYLVLKGKLGDD
                     GVYKLKQGNHNTALAEWVLRSNEVTKYLETDNRLIQKTEPIYMLPDHPFGRAQLYAPY
                     KYFNGQYIKTIWFNIIAIWLFTIALYTLLNLINNKKTGRVPVLWRVIGKFTR"
     gene            complement(11115..12230)
                     /locus_tag="PK049_06570"
     CDS             complement(11115..12230)
                     /locus_tag="PK049_06570"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HOK61258.1"
                     /translation="MNENAFDFNKLIADSKNTLLNPKGYFESMSTAGGMVEPLLKALI
                     YGVVAAIINAIWFFALGSLVTLGAFGGTFGIVAFIFTIIAAIIGLFIAAVIILLLSSI
                     SSGNTDFEANLRVSAALMVLMPINSLLAVFGFISGLLGTIVSIAIGLYGLYLLYLALT
                     KTLKGKEETARIIIYVLIALLVIGQIISYFSRRALQNFGKDLSRYEQAYGEKEEDQNA
                     DYNYYSNEKPDEFPSKALEVVKVHLSTGSGSITKEKIERLVKLTEELDKYESDNAKMM
                     ELVKEFGYVDISEYTNDYLVVISGITAVSSLNAMEQLMKASEKEKKAAEGFMVDEMLK
                     NAAIQSILSGKLTESDLVTVFNNWDTVKDLENKTVTE"
     gene            complement(12319..13653)
                     /locus_tag="PK049_06575"
     CDS             complement(12319..13653)
                     /locus_tag="PK049_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOK61259.1"
                     /translation="MAKILVVDDDSTFCLMLKTFLVRNGHKVTEAFSFDDARQAIQTT
                     TFDVVLTDFRLPEKSGMDVLTEAKRKNRDTIVIVMTGYGDIRMAVKAIKQGAYDYVTK
                     PVNPEEILATINNALPISVGKTPETDTDAFTFIEGKSRYSQEVMRYINIVAPTNLSVI
                     IQGDSGTGKEFVARQIHRASKRNSKPFVAIDCGALPRELAASEFFGHIKGSFTGAIAD
                     KIGQFEVANGGTLFLDEIGNLPYDVQVNLLRAIQERKIKRIGSSKEIPVDVRIIVATN
                     EDLLEMVNRGTFREDLYHRLNEFTIRVAPLAERKDDLMVYAQWFLNNANREMGKSIHD
                     FSPEAIDAMLQYSWPGNLRELKNAIRRAVLLEQSSSITVESLPTEIVSPSQKSSSTKT
                     DNLKNTKEKAEQELIIATLEKVKYNKTKAAKLLNIDRKTLYNKLKQYGIDTD"
     gene            13995..14459
                     /locus_tag="PK049_06580"
     CDS             13995..14459
                     /locus_tag="PK049_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61260.1"
                     /translation="MERIQIYKKQKGLEIYVKIFGLLFILFGMTFLIKSLMNGDKTDF
                     NQGDWNYLFYIIQVIQGAALFALGYSNKKNEKLFIEWDEKEMRYLLSKNKTIEKIKFE
                     DIRKVTIQLYEIKIELPSTEKVLNLKNIQYNELKKIKEKFEELKLDLEKRNG"
     gene            complement(15643..17952)
                     /locus_tag="PK049_06585"
     CDS             complement(15643..17952)
                     /locus_tag="PK049_06585"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOK61261.1"
                     /translation="MKKTFLFFAMAFFSLNTFSQVSLSGKVIDKETGNGIPFAIVEVK
                     DSDIKTVSQSNGAFLLQITGNNTIEITAKSLGYSSKSLVISPDSFNKEITIMLDAEIY
                     ELKTVVVTATRSERKILDVPQRVEVIGNNKIQGIPALSADNYLYSIPGVSISRGASIF
                     GAADISLRGMGNEAGRTLVMIDGAPINKNDGGTVNWNSINTANLKQIEVLKGSGSTIH
                     GGNAMGGVINLITPTPSEPLQGDVSLSYGSFSTLQTQANVSGIKDRLYWSINGSHRKS
                     DGYITSQADEVDEFSIPSFLTEYNVGAKVGFQLSAKHKIECSGGYYSGQRGTGSNFTG
                     YGFTNENLASSKGAYNWYTDINSRVSYQGIFNDNSNLKVNFYTQREYYKNIRESLKNN
                     EIKRYDVLSTRDDIGLLSGYSFSLGKYNKLTSGIDMRYGAVDGADTYITSTDKVLNLG
                     KMNLVGVYLLDEIKIGETPLSILAGIRYDYAMFYDGKFLVENPTSETAFLQNFSGDLN
                     NANFSAFSPRVSFQYYQPQKFRTYLGYSRGYRAPVLDDLCRTGRISGGMKIANPLLKP
                     EYIDNYEIGADILYFKKVSFSTSFFYSIGQDYHAYIATGDSIILNNKLRPIMTKSNIG
                     KVEIYGAEFNVNFTLSNSFIWSLAYSHIVTNILDYKRLNSEIEDNLVGKELVYQPSDI
                     FNTTLIWKNSIINSSVVFNYKGAQWINDINTEKINSYYFIDLQLWRPIYKGLQASLMI
                     HNLLNNHYIDSRNMISPGRMLSLQIKYEF"
     gene            18145..19224
                     /locus_tag="PK049_06590"
     CDS             18145..19224
                     /locus_tag="PK049_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61262.1"
                     /translation="MRKNITKILSALGFITISAACSYLPRTWIDALSGASITLSQNGN
                     SLFHQTDAKKLNISEISIQGEVKKTGVVDLRKFYKREVFVKEAILDESNDPKFIGAFR
                     YIGYSLFDLLNSYNLDKKNEKFFRPSIDVYVVISNDKGEHVSFSWSEIFHTINPHQII
                     IATEVAQIKPHKKEVNYLTGNCWKVVAANDLFSYRTIENPTSITIKSFDKKEYPINRE
                     IKPMYSPAVNVVFDNGFDFSIPTISDTSRIIKYTSIFVGMGMGYHPNPEFKGIPLKSL
                     MKKPNNLFDREWIMNGLVCFSSIDGYRAIYSYSELFNRSDQVIPILSIPENVEDGGYY
                     RIYLPTDFFADRSVKSLKEIYFFKD"
     gene            complement(19663..21816)
                     /locus_tag="PK049_06595"
     CDS             complement(19663..21816)
                     /locus_tag="PK049_06595"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HOK61263.1"
                     /translation="MDVKEMAKQHKMLNQWKYEWKPLYRGYTDKTLYINVGTNEVKEK
                     PVPPIMKEKFIGGKGYDLRLLWDATKPNTKWNDPENEIVISSGPIGGITQYSGTGKSI
                     VCTISPQTDIVIDSNVGGFFGPFLKFSGFDALEIQGKAKNDVIIYIDGVEHKVEIFEA
                     PAEPLDSHILGEVLSEMFANDEADKKNIGIVSTGAAADHSLIGMLNFTFYDPKRKCIR
                     LKQAGRGGIGTVFRDKKIKAIVCKIPGVKGNLNNVADLEAIAERGRRFNREIRELDDK
                     QCQMRSKGTAHLVEIMNDYDLLPTHNFQYGSHKDAHKIHSDVWKSYFTQGVPDGCWIG
                     CNMSCAKGVDKFEIKTGPYKGQKVIVDGPEYETAAGLGSNCGIFDPEYVIEANFYCDT
                     YGICTITWGTTMAFIMECYERGILNKERTGGLELRFGNKDAALELLHQVARGEGFGKL
                     AGMGVKKLKEHFVKEYGADEKFLNDIAMVNKGLEYSQYVSKESLAQQGGYAMTNKGPQ
                     HDEAWLIFMDMVNNHIPTFEDKAEALHYFPMFRTWFGLQGLCKLPWNDVEPENNAETD
                     EPAKVPEHVDNYVTIYNAVTGSNIDKHELIRQSERVYNFQRIFNIRRGYGTRKYDAQP
                     YRAAGPVTKEEYLSREERYDKQLKEQVGVDPTKMTLEEKMAALRKYREDRYEKLLDAV
                     YERRGWNKNGVPTIEHLKKIGMDLPELIEVVKPLQ"
     gene            22143..22739
                     /locus_tag="PK049_06600"
     CDS             22143..22739
                     /locus_tag="PK049_06600"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOK61264.1"
                     /translation="MNQLEDSHKGMRENIVSAATEVFSRYGFKKTSMEDIARALRMGK
                     SSIYYYFKGKEEIFQAVVDREANLLRVKVKEILESGLPVTEKLRSYVKMRMDLIKQLS
                     NYMEILKNDDLMNLELTEKFRKKYDDEEITIVKQMLEEGINRNELKVKDLNLSALAIV
                     TAMKGLEIPLVTSTMGVESLNMVIDDMLDILFYGIVKR"
     gene            22752..>23533
                     /locus_tag="PK049_06605"
     CDS             22752..>23533
                     /locus_tag="PK049_06605"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOK61265.1"
                     /translation="MKILRLVSFFGAWLIGIGYVSGQQVLTLEQCRQMALEHNQKVLI
                     ADEHVEAAGALKRSAQTQFLPSISANGLYTRTNKKFSLLDKDLMLPVIPYTAIDPVTG
                     GLNSAILSPTLPDGTPNPNFNPSVFGSTFAIDPSTGQPYTDSDGNPLFQRYTWLPKDK
                     AEFGNKNVYVAGVNLTQPIFTGGKIRETYRIAKYGEKLARAQSEAERTEVLYKTEESY
                     WRVAAVAEKVKLVQAYIALLDKLNTDLENYYAEGIIIKNDLL"
BASE COUNT         6793 a         4858 c         5068 g         6814 t
ORIGIN      
        1 gttcctccgg gcagcagccc tgagatgttt gagccaaccc ctcagcaaat ggctgagctt
       61 acacaggcta aaggctactt ttccataggg ctactcgatt ttgagaaggg aatggagcaa
      121 aaactaaagg ataatgcaga attcaggtat atcgacctgt cgcagggcct tgagctgatt
      181 aagggtgaat gtgagcacaa ccacaatcac gaaagccatc aggggcataa ccatgccgtt
      241 gacccccata cctggatgtc ggctgccaat gcaagggtaa tggctaaatc catagcccaa
      301 agcctaaccg cacttttccc cgagagggaa agcgagttca acgccaacct caaccgcttg
      361 gttgcccata tcgattcggt tgataatgag gttaggggta ttctggcagg ttccgggcgt
      421 aaaacctttg taatatacca tccagcttta ggttactttt cccgcgatta cgggttaacc
      481 cagctaagcg ttgagcacga agggaaaagc ccatcggcca aaggggtgat ggagctggtt
      541 gagcgctgcc gtgccgaggg tatcacaacc attctatcgc agagccagtt cgatacgcac
      601 aactctcaaa ccattgccaa tgagcttggc ggtacagtgg ttaaggttga tccgcttcag
      661 gaaaactggg ctgagagtat gatttttatt gctaacgcaa ttgcaggtaa tgtaaaatga
      721 caaaccaaac cgacaagctg atagagctgg ttaacgttac agctggatac ggtaaaaaca
      781 ttgttttgcg taatgttaac ctcgccatca acactaacga ttttattggt ataattggcc
      841 cgaatggtgg agggaaaaca accctaataa aactactaat aggtcagcta aaacccttta
      901 ccggagaggt tatttaccac acaaagggaa acggttcact aattggctac ctgccacagg
      961 caagccagat tgataaaaat tttcccatat ccgtaaggga agtagtgcta tcggggcttt
     1021 tggagaaaaa ggggattttt ggcggctaca gccgccaaca aaagggtgtt gcccttgacc
     1081 tgatgaaaca ggtaggaatt gcgaaccttc agaataaaag tatagggcag ctctcgggtg
     1141 gtcagctgca aagggcattg ctttgcaggg cattgatatc ggccccacag gtgcttatcc
     1201 tggatgagcc caacacatac gtggatagca actttgaggg ggagctttac gagatgcttc
     1261 gcaagctaaa cgagcgaatg gctattgtaa tggtatcgca tgatttgggt actataacct
     1321 cgtatgttaa aaccattgct tgcgttaacg gtacactctt ttaccaccaa tcgaaccaga
     1381 ttaccaacga gcagcttgca gcctataatt gcccaatcca aattattgct catggtccgg
     1441 ttccccatac cgttctgctc gatcatggta aagaaaatca acataatcac aatcactaat
     1501 tatccctgtt atgattgagg ccgttaaaca gttctttcag tatgcattta ttcagaatgc
     1561 aataatttca gcattcctgc tttcggtaat aaccgggttc attggttcgt acattgtggt
     1621 taggcgattg gtttttctga gcggtggcat tacccatgcc tcctttggtg gtattggtat
     1681 agcttggtat tttgggctaa accccatcct aggggctact gttttttcag tattttcggc
     1741 actggggctt gagtacttat cgcaggggcg aacgctgcgt gaggattcgg caatagggat
     1801 gctttggtcg tttggcatgg ctgtaggtat tctttttgtt ttcctaacgc cgggttatgc
     1861 tccaaaccta atgtcgttcc tgttcggaag catactaacc atttcccaaa ccgatatatt
     1921 actgctgctg atacttgcca cgcttacgct agtatttttt gtggcttttt actacccaat
     1981 tctttttgtg gcattcgata gcgattttgc caaaacacag aatgtcccgg ttaagttcat
     2041 tagctactcg ttggctgttc tcattgctct ctcaatagtt tttgccataa gagctgtggg
     2101 catcattcta atcatatcgc tgctaaccat acctgcaaac atagccaata tagtaaccca
     2161 tcggtttaga aggataattg tgttatcggc cttaatcagc cttttttcga ttgttgcggg
     2221 tatagctatc tcgttcaggt tgaatgttcc ctcgggtgca acaatcatta ttaccatgat
     2281 ggctttctac cacattgcaa aagggattca attggtaagg cagcggattt ttacactgaa
     2341 agtgtaaatt gttaaacctt acggtaatcc tcctccagca gctggcaaac aaccttaatg
     2401 gcattgccac acaccatatc gaacaggcct gcatcgcggg ccttttgttt tgcttcgggg
     2461 ttgttcaaat ctattcctag tattctgcag caatcggttg accctaatcg tttggcacat
     2521 ttatccatgt actcggccac aagtgtggtt gctttttgct tggagtcgga atcgttcagg
     2581 ttactattgc caaaccttaa tccgataatc attgcccctg cgttaattgc tccgcacaca
     2641 tgttgcttat gaccaatacc cccacccagt ccagcaccta gcttcaagca ttgctcggta
     2701 gttaacccct cgtcagtggc aaatgcaagc aatacagact gtgcgcaatt ggcgctcccc
     2761 tgaaaactcg atagggcaat ggatatttta tctcgcataa aatctgcagt tttagttcat
     2821 ttacgaatag gtttcccaaa ttgtttctgt ttttttataa ctcatcatgt ttatattgat
     2881 tagcagcact tgtattaatc gaaatcaatc tgataatgcc caaatattat atgaacaatg
     2941 atattagtga actattaatg cgatgtatga catctaacat tttcccattc catttgttat
     3001 tgtaattttg acaaacaaaa atttgactct atgaacttcg accttatagt aattggaagc
     3061 ggccccggag gttatgtggc cgcaatacga gcaagccagc taggcatgaa agtggctgtg
     3121 gtggaaaagg aaaaccttgg aggtatatgc ctgaactggg gctgtatccc caccaaagcg
     3181 ttacttaaga gcgcacaggt ttttgagtat gcatcgcatg ctgccgatta tggagtgttg
     3241 gcagccgatg taaaacccga ttttgaaaaa atgattgctc gtagccgcgg tgtggccgat
     3301 gccatgagca agggaataca attccttttc aaaaagaata acatctccgt tattaacggg
     3361 tttggtaagc taaaggataa ctttaccgta acagtaaccg cttccgatgg tacacaagcc
     3421 gactacactg caaaacacat catacttgcc acgggtgcgc gttcgcgcga gctaccaaac
     3481 ctaaaacagg atggggtgaa ggtaattggc taccgtcagg ctctcacttt acccaaacaa
     3541 ccggcctcca tggtggttgt tggttcaggt gctattggca gtgagtttgc ttacttttac
     3601 aacgcaattg gcaccaaggt aactttggtt gagtatatgc ccaatattgt tcccctggag
     3661 gacgaggagg taagtaaaac ccttgagcgc gccttcaaaa aggctggcat aaccgttaaa
     3721 accgaagcgt cggttgaggc ggtcgataca tcgggcgaac tatgtaaggt taccatccaa
     3781 accaaaaagg gccctgaggt ggttgaggcc gaggttgtgc tttcggccgt aggcattacg
     3841 cccaaccttg agggaatagg tattgaggag ttgggtatta ttctggacaa gggcaaggtt
     3901 aaggttgacg agttctaccg caccaacgtt gagggcattt acgccattgg cgatatagtg
     3961 ccgggtcctg ctttagccca cgttgcttcc gccgagggta ttgtttgcgt tgaaaagatt
     4021 gccgggctta acccccatcc aataaactac agcaacattc ccggttgcac ctacaccacg
     4081 cctgaggttt cgagcgtagg tatgagcgag aaagcagccc gcgatgctgg ttacgagatt
     4141 aaggtgggca agtttccttt tacagcctcg ggtaaggcaa ctgcagccgg taaccgcgat
     4201 ggctttgtga agctcatatt tgatgccaag tatggtgaat tgcttggcgc ccacatggta
     4261 ggtggtaatg taaccgaaat gatagccgaa atggttgtgg cacgcaacct cgaaaccact
     4321 ggtcatgagc taatcaagag catacatccg cacccaacca tgagcgaggc aattatggaa
     4381 gccgctgctg ctgcttacgg cgaagcaata catatctaaa tttactttga tattaccaca
     4441 aaaccgctac gtttcccgtg gcggtttttt gtttttatga aaatctacgc tttttctcca
     4501 tgaatgtccg tggctgcctg ggtttccaac tcttaatcaa aaaatagatt acagggccta
     4561 gtattggcgg gaaaaatgcc aaaacaacaa ttatgggagt cctctgcttt ttcaggtttt
     4621 tcagcacatc aaaaaaagta aacagatgta acccaatgat tagaatgtat gctaccgttt
     4681 caatacttat attccacata cttattgcat ttaaccacaa agataatcaa tatggttgtt
     4741 aactcatcga taaataagtg tgagaagtaa tgtacctaaa aaacgagtaa aaatcaacta
     4801 acaacgaaaa cccacttatt aacaccttta gttaatcact ttgctgtaac tgttcataaa
     4861 cataccctga aaatatcgag ccataaaatc aacttacact atctttacaa aagaaaaagg
     4921 ccaaaaatgt attgctaaat gattgaccag catacagttg ataaaatact ggcagcagcc
     4981 gatattgttg aggtggtaaa ggaatacgtt acccttcgga aacgcggggt taattaccta
     5041 ggcctttgtc cattccataa tgagaaaacg ccctcgttca ccgtgagtca ggcaaagggt
     5101 attttcaagt gctttggctg tggcaagggt gggaatgtgg tcaatttcat tatggagcat
     5161 gagcggctta actacgtgga ggctcttaag ttcctggcca agaagtatca cattgagatt
     5221 gaagagaaag aggttacccc cgaggagata gagaagcgga acgagcgcga gagcctaatg
     5281 gttatcaact cgtatgccca gcgctacttt tccgatatgc tgcataagca ttccgatggc
     5341 aaaaccattg ccatggctta cttccgcgag aggggattta aggaccatat cattgagagg
     5401 tttcagctgg gttactgttt ggaccagcgc gatgccttta cccaaagcgc acttcgcgat
     5461 ggtttcaagc tcgactacct tgttaaaacc ggacttacca taaagcgcga cgacggcagc
     5521 acctttgatc gctttgccgg tagggtgatg tttccaatcc acagcataag tggcagggtt
     5581 attggttttg gggggcgaat acttaaaacc gataaaaaaa cggctaagta ccttaacagc
     5641 cccgagagcg aggtttacca taaaagcgag gtgctttatg gcattttcca tgccaaaaaa
     5701 gccatcaccc aggagaacaa gtgttacctg gtagagggtt acaccgatgt aatatcgttg
     5761 caccaaactg gcattgagaa tgtggtggcc agttccggaa catcgcttac gcaggatcag
     5821 attaagctga taaagcgctt taccccaaac gttaccattc tttacgacgg cgatgccgcc
     5881 ggcattaagg cctcgctcag gggtatcgat atgatactgg aggaggggct taacgttaaa
     5941 gtggtgctta tgcccgaggg cgaagacccc gacagctttg cccgttccca taacgcctcg
     6001 gaggtgctgg agtacatcaa ccaaaacgaa accgatttca tcaaattcaa gacccgcctt
     6061 ctgctcgatg atgccaagag cgatcccatt aaacgcgcta atcttattac cgatattgta
     6121 cgctcaataa gtgccatacc cgaccaggtg attcgggcgg tttacatcaa ggagtgcgcc
     6181 aagttaatgg atattggcga ggatgtgctt tactccgagg ttggcaagct gcgtaggagc
     6241 agaatggagc agctgctcca gcgtgaacgc accgaggcgg tcagggaaca cgaaacaccc
     6301 aaaattcctg cttttgttca aggaattagc tgcgaggagc aggaaaagga gattgtacgg
     6361 tttctgctga atagcggaaa ccaagtgcta tttagccagg aggtggagga aaccaatgag
     6421 cttatcacta ttactgctgc tcagtatatc attgatgaga ttcttaacga tgatttagag
     6481 tttcagaatt tgatttacaa aaaggttttt gatgaatacg tgcgtttgct ggaaacaaac
     6541 gttgaggtcg ataaccgatt ttttattaac cacaccgatc ctgaaattag ccaaatggcc
     6601 gtggatttac ttaccgaaaa atacgtgctt agccggattt gggaaaaaca caaggccgag
     6661 gttactgaaa gccccgattt tctacaaaca gccataccca aggctataat ggtttacaaa
     6721 agcaaagtgc tgaagctggc cattagcaag ctaaccgatg agctgagtaa gctcaaaccc
     6781 gatcaggttg aggaaaccaa tcagttactc ctacggctca gggagcttta tgccctaatg
     6841 aataagatgt cgaaggattt agatagggta attctgtagc aattttagca gtttacataa
     6901 gcataatgac accttccctt aaattctgct aactttttta agtgtttttc ttttgttatt
     6961 attgttttta gtaaatttac aaaaaccaaa ttagggtttc ggttgcttta aacctttgcc
     7021 ggttatactg gttcaaacat attaaagcaa ttgtggttat tgtttatgtc atgttttttg
     7081 ctgaacctaa atagtttaac ctttaaacat ttataaatat gaaaaactac actaacaccc
     7141 tacgagttct tacgttagcc atgctggcaa cagttttttt cagctgtggc aataagcagg
     7201 caaagcaagc cgaagaacta caagccgagt caaaggagca agcttttcag gaagtaacca
     7261 gctatccaat tccaacctct tttgaagtaa ttcaaatgat taataaggcc ggagcaagtt
     7321 ttattattgg catttgtaac ccggttgata atgttgggaa atacatgacc gaaaaggcta
     7381 aggccgttaa cctgggtatt tacggtgctg accttgccta ctcaacaacc taccagatga
     7441 aacaggaaac catgaatttc cttaaggttt gtaaacagct tgtagaggag cttaacataa
     7501 atgccggatt tacccctgaa ctggcattaa atgttgaaaa gaatatcgac aataaggatt
     7561 cattgatagt tatttttacc aattcctact atgaaaccta ccgcaccctg gttcaaaatc
     7621 aaaaggaaag cctctcgctt ttggtagttg ctggtagctg gattgagggt gtttatatca
     7681 catcgcagat tgctttgaca agtcgcgaca acactgagtt tatcaaaatc cttgcaaacc
     7741 aaaaagcccc acttggcaag ctgatggagc tcatgactcc ccgtgtagat attcctgagg
     7801 ttaaggaact gatggatatg cttgccccca ttcaggctat ttacaataag gttgagggtg
     7861 aaaaattgac caacgatcag tttcaaaagc taatggaaga aattacaaag gttcgtaatg
     7921 ccttggtata attcattttt ataaaccatc attaaaacgc accccaaaaa ggtgcgtttt
     7981 tttatttttt tcgtgtaact tttaactttt taaactatct aattagttag aaagaaattg
     8041 caaaaagagt ttggataaga atattgccat aggacttatc gatacgctag ccgatttaac
     8101 tcgcaaggac tgggatattg agcaatgccg gagcattatt caaagcttct tttcatcacc
     8161 cgattttgat gttaatggag aggagcttct taagcagttc gatagtatca ttagctcaga
     8221 gagtttcgtt agcggtaatc ttaaacacga tgccggtttg ccctgcgaaa cgatgaccac
     8281 cgaacagcgt attgaattgg ccatagcatt ttaccatgta ctctcttcgt atcctgaatc
     8341 tgggcatcaa aagcttttta ccgcatggaa tgtaattggc attcagccag aggatgcaat
     8401 ttgcctgtac catttcctaa gctttggccc atccgctaat caatttcagc ttccaggcaa
     8461 ttcttttatt tcacaagatg ttcttgaggg ttgggagcaa aacaatagca ataggatgat
     8521 tttcctatac gatgaaaatt cccctaccca agaacttgat aaagcattat gcgagaaacg
     8581 tactggactt ggaggcaatc tactttttgc ctacttccat agcataggct gttacatagt
     8641 taagtaccat ggcgatagac aggttttgat tgaagatgaa ccacttatag ctggacgttc
     8701 gtactgtttt aagccgggtt caagcattac gcttagcaat ggtgagcaaa tccgatacga
     8761 cgatataaca cgtatacgct tgctgggtag cggcgttcag ccggttaaat tggttgttca
     8821 ggatcttgag tttacttttc ccggaagcaa ccagggcatt aagcccttta gtaccgttga
     8881 ggagtcgggg agggtagtgg gaatacttgg cggtagcggg gtgggtaaat caaccctact
     8941 gaacctcctg tcgggcaagc ttaagcccca taggggcaaa gtgctgatta acggctacga
     9001 cgttcataac gagtcgcata agctgatggg tgttgttggc tttgtacccc aggacgactt
     9061 gcttattgat aacctctccg tttaccagaa catgcttttt aatgcaaggc tttgctttgg
     9121 caactatagc cgtaagcagg ttagggagct tgtggattct actttaaaaa gcttggattt
     9181 atgggaaatt aggaatttaa aggtagggaa tgctctggat aaggttataa gcggagggca
     9241 gcgcaagagg ctaaacattg ggcttgagct gctacgcgag cctgcggtgc ttttcctcga
     9301 tgagcccacc agcggcctat cgtccaacga ttcggttatg gtacttaacc tgctccgcaa
     9361 ccttgccgat gccggcaagc tggttgtggt aaatatacat caaccctccg aaaggctttt
     9421 cagaatgttc gataggctat gggttttgga taaaggcggg tatccggttt atgtgggcaa
     9481 cccaaccgag gcggttggtt actttaggga gaaatcggct agggttggca acctggtgac
     9541 atcagcagtg caaaggaaaa gggttaaccc tgaggatata ctcgatatta ttgagcagcg
     9601 tcaggttgat aggagcggtt cgtttactgt aaaccgtaag gtatcgcccg aggagtggta
     9661 taggctctac cgtgaatcga ttcagcccaa agtgattgag attgagggga aaacggcatt
     9721 accccagggt aagtttcggt tacccgacgt aataaagcag ctaaacgtat ttactaagcg
     9781 taacctgcta tcaaagctgg ccaacaggca atacatgttg ctcaatatcc tggaacccgt
     9841 tgttctgggg cttatccttt ccttctttat taagtactcg ccaggcaacg attacatttt
     9901 tgcggcaaac cgcaacatac ccgcttttat atttatgagc gtgatagttt cgctctttct
     9961 gggccttagt gttagcgccg aggagataat tggcgaccgc cgcattctgg agcgtgagtc
    10021 gtttcttaac ttgagccgct tcagctacat caactcaaag gttctttacc tgtttggtct
    10081 ttcggccatt caaatgctac tgtttgtagg ggtgagtctt accattattg gagttaaagg
    10141 tttaacgctc aagtactggt tggtgctttt cagctcggca tgctttgcca atatgctcgg
    10201 gttgataatc agctcagtga tgaaatcggt tgttgccata tatattacca taccgttgct
    10261 gcttgtgccg caaatccttt taagcggaac ggtggttgat ttcgataacc taaactcatc
    10321 gctcactcgc agagtttacg ttccggttat tggcgatgtt atgacatcgc gatgggccta
    10381 cgaggcgctt gcggtaacgc agtacgtgga caaccgttac gaaaagaatt tctttgatgc
    10441 cgagatgcag cttagccgtg ctgcttttag ggcgtcgttt cttattcccc gcctgcaggt
    10501 taaacttgag gaatgcgtga ggctcgatga ccttgatgaa gataggagtg ccgatatatc
    10561 gcgccatttg cgctttattg ccaatgaaat tgaacacctt gcccaatccg acgatgtacc
    10621 tcagtttgag ttactggtag acctaaaaaa gggtcagctt tacgaccagc ttgcttttga
    10681 gctgaatggt tacctacttt acatcaaaaa gctttttcag gagcagcagc gtgccgccac
    10741 tgccaaacgc gatagcgttt acctggttct taagggcaag cttggcgacg atggcgttta
    10801 caagctgaaa caaggaaacc ataacactgc tttagccgag tgggtgctaa ggagcaacga
    10861 ggttacaaaa tacctcgaaa ccgacaatag gttgattcag aaaaccgagc ccatttacat
    10921 gttacctgac catccctttg gccgcgcaca gctgtacgca ccctataagt acttcaacgg
    10981 gcagtatatc aaaactatat ggttcaatat aattgccatt tggcttttta ccattgccct
    11041 atacacgctg ctgaacctta ttaacaataa aaaaactggc agggtgccgg ttttgtggag
    11101 agttattgga aagtttactc ggtaacagtc ttattctcga ggtctttaac cgtatcccag
    11161 ttgttgaata ctgtaaccaa atccgattcg gttagcttac cgctcagaat tgattgtatg
    11221 gctgcgtttt taagcatctc gtcaaccata aagccttcgg ctgctttctt ttccttttcc
    11281 gaggctttca ttagctgctc catggcattt aagctcgata ctgctgttat gcccgatatt
    11341 acaacaaggt aatcgttggt gtattcggaa atatcgacat agccaaactc ctttaccagc
    11401 tccatcattt tggcattatc gctttcgtac ttatcgagct cttcggtaag ctttactaat
    11461 ctttcaatct tctcctttgt tattgaacca ctacccgttg aaaggtgaac cttaaccact
    11521 tccagcgctt tgcttgggaa ctcatcgggt ttctcgttgc tgtagtagtt atagtcggcg
    11581 ttttggtcct cctctttttc gccataagcc tgttcataac gcgataaatc cttaccaaag
    11641 ttttgaagag cccttctgct aaagtaacta atgatttgtc caattactaa aagggctatt
    11701 aaaacataaa taataatgcg ggctgtttcc tctttccctt ttagtgtttt ggtaagagca
    11761 aggtaaagca gatataatcc gtataagcca atggctatcg atactattgt tcccagtaag
    11821 ccgcttataa acccgaagac tgcgagtaaa gagttgatgg gcataagcac cattagtgct
    11881 gcgcttaccc ttaggtttgc ctcaaaatcg gtattgccac tacttatact cgatagcaga
    11941 agaataatta cggctgcaat aaatagccca attatggctg caattattgt aaaaataaac
    12001 gctacaatac caaatgtacc accaaatgcc ccaagtgtta ctaggcttcc cagggcaaag
    12061 aaccaaatgg cgtttattat tgcagcaact actccgtaaa tgagagcctt gaggaggggt
    12121 tctaccatac cgccagcggt actcatcgac tcaaaatacc ctttggggtt aagcagggta
    12181 ttctttgaat cggcaatgag cttattgaag tcgaaagcat tctcgttcat agttattagt
    12241 tttttgggtt caagaacgaa agttctgtta aaaaaacgag aaagtcaact ttaggcaaat
    12301 atttatcaag ctagggcttt aatcggtatc aataccgtac tgcttaagct tattgtagag
    12361 cgtctttcta tcgatattaa gcagcttggc ggccttggtt ttgttgtatt taaccttttc
    12421 taaagtagcg ataatcagct cctgctcggc cttctccttg gtgtttttaa ggttatcggt
    12481 ttttgtggag ctgctttttt ggctgggcga tacaatttca gtgggtagcg attcaacggt
    12541 aatactgctt gactgctcaa gaagcactgc cctgcgaatg gcatttttta gctccctgag
    12601 gttaccgggc cagctgtact gcagcatggc atcaatagcc tcgggcgaaa aatcatggat
    12661 actctttccc atttccctgt tggcattgtt caggaaccac tgggcgtaaa ccatcaaatc
    12721 gtccttgcgc tcggctagcg gcgctaccct gatggtaaac tcgttgagcc tgtggtaaag
    12781 gtcctcgcgg aatgttccgc ggttcaccat ctcaagcagg tcctcgttgg tggcaacaat
    12841 tattcgcacg tcaacgggta tctccttgct gctgccaatg cgctttattt ttcgttcctg
    12901 aatggcacgt agcaggttga cctgaacatc gtagggtagg tttccaattt cgtcgaggaa
    12961 gagagtaccg ccattggcca cctcaaactg gccaatcttg tcggcaatgg ccccggtaaa
    13021 cgaacccttg atatgaccaa agaactcgct ggcggccagc tcgcgcggta gcgcgccaca
    13081 gtcgatagct acaaagggtt tgctgttccg tttgctggcg cggtgtattt gacgggcaac
    13141 aaactccttg ccggttccgc tatcgccctg tataataacc gataggttgg tgggtgcaac
    13201 aatgttgatg taacgcatca cctcctggct gtaacggctt ttgccctcaa taaaggtgaa
    13261 agcatcggta tcggtttcgg gggttttccc caccgagatg ggcagagcgt tgtttatggt
    13321 tgccaggatt tcctccgggt ttacaggctt ggtaacgtaa tcgtaggcac cctgcttaat
    13381 ggccttaacc gccatgcgta tatcgccata gccggtcatc acaattacaa tggtatcgcg
    13441 gtttttacgt tttgcctcgg taagcacatc cattcccgac ttctcaggca ggcggaaatc
    13501 ggttagtacc acatcgaacg ttgtggtctg aatagcctgc cttgcatcat caaacgagaa
    13561 ggcctcagta accttatgcc cgttccttac cagaaaggtt ttaagcatca ggcaaaaggt
    13621 agaatcgtcg tcaactacaa gaatcttagc catttagttt atcccattca ccaacgctaa
    13681 ggtagaaact attttggaga aatagaaatt taagtgtttg gagtatggtg tttagtgctt
    13741 agttaataaa aaaaatatgt aaatcaagca aagcagttta atttgagccc cactcattca
    13801 atcatctact aacaaattca tttccaggta aaatggtcta aaaaaaatta aaaagtcctt
    13861 ttgatgattt atgaaattat tttatttttg aataaataaa ccctacaaaa agtacggtta
    13921 tacacccaaa atagatttat aaaattactt ttggtacggt tataaacgag ttagagcaaa
    13981 taataagaaa cgatatggaa agaattcaga tttacaaaaa acaaaaaggc ttagaaattt
    14041 atgttaagat ttttggcctt ctatttattc tttttggaat gacttttttg ataaagtcgc
    14101 taatgaatgg ggacaaaaca gattttaatc aaggagactg gaactattta ttttatatta
    14161 tacaagttat acaaggtgca gctttatttg ctcttgggta ttctaataaa aagaatgaaa
    14221 aattattcat cgagtgggat gaaaaagaaa tgagatactt gctatcaaaa aataaaacga
    14281 tagaaaaaat aaaatttgaa gatattagaa aagtaactat ccaactttac gaaataaaaa
    14341 tagaattacc atcgactgag aaagtactaa atttaaaaaa catccaatac aacgaactta
    14401 aaaaaattaa agaaaaattt gaagaattga aattggattt agaaaagaga aatggttaaa
    14461 ttacttgcgc taacaggcag ttcctttcac tagggctgat gatgcagata cgaaagtttt
    14521 tagtaattta gtttcgtttt aacgtgggat gacgccgctg gctcttaact ccccagcagc
    14581 ccaaaccacc gaccgttgta attaaagaaa tgcaatgaat ttaggtttat agtaagagat
    14641 atcgataaat tttttgaaag ttttcagaag attgctctga aggttaacag gattttagaa
    14701 tataaaaatt actttagttc atatttcgac tataaaacga ttttattaaa atgaataagc
    14761 atatttactt caccctattg tttataatta tatcatttta tcatgtaaaa accatgaata
    14821 tagatgcaac acgaatacta tttcaggtaa tgataaagga ttaaaaaaag cataaaatag
    14881 attagtataa ataatagaat aattgaaaaa tcattaataa ttgaaaaaga aatagcaaag
    14941 cagcacaatt ctgtagaaca attacaaaat ttgctacccc ctgaaattgg cgataaatat
    15001 catgatttta gtggagtaaa tagtaatggc gaatttatga agatttcaga tattaaaggg
    15061 aaatatattc tattgaaatt tacaggatct ggttgtggtg cttgcgtaaa atctatacct
    15121 gaactcagaa gggtatatga aaaatatcat aaatcacttg aaatagttga ttttactctg
    15181 acgtaagtgt atatgactgg ttaaagcatg ttgaaaagga aaaaattcct tggctaacat
    15241 tatgtaacgg aagaggaatg tatagtaata cttgggagaa ttacgggata aagggaatac
    15301 ctacattttg ccttattaac aaaaaaggta ttattattca aaaatggatt ggatacgaat
    15361 aatggctaat agaagaacag attgaattaa taatagaaaa taataatgga gcccaacaag
    15421 ggatgtaacc attttgggtt gaatggatag gcaagcgggt tggcacgcag gaactcctta
    15481 aagtggaata tatctcaagc cccgtacccc caaactggtc acaccccaac cgttggacac
    15541 aagagtatta aacgacgaat aaatcgtctt gtaaacgtta atacttaaac ctatttgatt
    15601 aaaagcaaga aaaacttgac tttatcattc attttaacgc acctagaatt catattttat
    15661 ttgcaatgac agcatacgac caggtgaaat catatttcgt gaatcaatat aatgattatt
    15721 aagtaaatta tgaatcatta gtgaagcttg caagccttta tatataggcc tccatagctg
    15781 taagtcaatg aaataataac tattaatttt ttctgtatta atatcattaa tccattgcgc
    15841 tcctttatag ttgaaaacaa ctgatgaatt gattatagaa tttttccaaa ttaaagtcgt
    15901 attgaatata tcacttggtt gataaactag ttctttccca actaagttat cttcaatttc
    15961 agagttaagt ctcttataat ctagaatatt agtaacaata tgactgtatg ctaaagacca
    16021 aataaaacta ttgcttaacg tgaaatttac attaaattct gctccataaa tttcaacttt
    16081 tccgatattg cttttagtca taattgggcg aagcttatta ttcaaaataa ttgaatcgcc
    16141 agttgcaata tatgcgtgat aatcttgccc aatagagtag aaaaatgatg ttgaaaaact
    16201 taccttttta aaatacaaaa tatctgcacc aatttcgtag ttatctatat attcaggttt
    16261 tagcaaagga ttagcaattt tcattccgcc tgaaatacga cctgtacggc acaaatcatc
    16321 aagtacagga gctctgtaac ctctgctata gccaaggtag gttctgaatt tttggggttg
    16381 gtaatactga aatgagaccc ttggactaaa agcagaaaaa tttgcattgt tcaaatctcc
    16441 cgagaaattc tgtaagaaag cagtttcact tgttggattt tcaactaaaa atttaccatc
    16501 gtagaacatt gcgtaatcat atctaatgcc agctaatatg ctaagcggtg tttctccgat
    16561 ttttatctca tcaagcaggt aaacacctac taaattcatt ttccctaagt ttaaaacttt
    16621 atcggtagat gtaatgtaag tgtcggcacc atcaacagca ccataacgca tatcaatacc
    16681 tgaagttaat ttgttgtatt tgcccaatga aaaactgtaa ccagaaagta agccaatgtc
    16741 atcacgagtt gaaagcacgt catatctttt tatttcatta ttctttaaac tttctcttat
    16801 gttcttatag tactcacgtt gagtataaaa attaaccttt aggttgctgt tgtcattaaa
    16861 tataccctga taagaaaccc tgctgtttat gtcagtgtac cagttataag cacctttgct
    16921 agaggctaaa ttttcatttg taaaaccata acctgtgaag tttgaaccag taccacgttg
    16981 ccctgaatag tacccacctg aacattcaat tttatgtttt gcagaaagtt gaaatccaac
    17041 ttttgctcca acattgtact cagttaagaa tgaaggaatt gaaaactcat caacctcatc
    17101 cgcttggcta gtaatatatc catcgctttt cctgtgactt ccatttattg accagtataa
    17161 tctatctttt atacctgaaa cgttagcttg tgtttgaagt gttgaaaagc taccataact
    17221 tagtgaaacg tcaccctgta gcggttcgct tggcgtagga gtaattaaat ttataactcc
    17281 gcccattgca tttccaccat gtattgttga cccagagcct tttaaaactt ctatttgttt
    17341 taaattagcc gtattaatag aattccagtt aactgttccc ccatcattct tatttatggg
    17401 tgcaccatca atcattacca atgttcgacc tgcttcgttg cccataccac gcaacgaaat
    17461 atcagctgct ccaaaaattg atgcacctct acttatggaa acgcctggga tggagtacaa
    17521 gtaattatca gccgaaagag cagggattcc ctgaatcttg ttgttaccaa taacctcgac
    17581 cctttggggt acatctaaaa tttttctctc gcttcgggtt gctgttacaa ctacggtttt
    17641 taactcatat atttcagcat caagcattat tgtaatttct ttattaaatg aatcggggct
    17701 tataacaagt gacttggatg agtatcctaa acttttagct gtaatttcaa ttgtattgtt
    17761 acctgtaatt tggagtaaaa atgccccgtt actctgtgat actgttttga tatcagaatc
    17821 ctttacttca acaattgcaa atgggattcc atttcccgtt tccttatcaa ttacttttcc
    17881 gctgaggcta acctgcgaaa aggtgtttag gctaaaaaac gccattgcaa aaaataaaaa
    17941 ggtctttttc atttaaaatt gtttttaaaa ggtgaaattt aaacaactga atatctgact
    18001 taaaattgaa gcaacactaa tgttaatagg caaccctaag cgaaaaaaat tttttcagta
    18061 tgtatcattt gcttctggaa ttttttttgc acttttgcga tatgcatata tttgcataac
    18121 gcaaaaataa caaaagattg gtatatgaga aaaaatatta caaaaatttt aagtgcactc
    18181 gggtttatta cgatttctgc tgcttgtagc tatttgccaa gaacttggat tgatgcatta
    18241 tcgggtgcat ccattacatt atcccagaat ggtaatagtt tattccacca aactgatgca
    18301 aaaaaactga atatttcaga gattagcatt cagggggagg tgaaaaaaac aggagtggtt
    18361 gatttaagaa aattctacaa gcgggaagtt tttgtcaaag aagctattct tgacgaaagc
    18421 aatgatccta aatttattgg tgcttttcgt tacattgggt attctctttt tgaccttttg
    18481 aactcttata atcttgacaa aaagaatgaa aagtttttta gaccttcgat tgatgtgtat
    18541 gttgttattt caaatgataa aggggagcac gtttcgttca gttggtcaga aatttttcat
    18601 actattaacc cacatcagat tattattgcc actgaagtgg ctcaaataaa gccacataaa
    18661 aaagaagtaa actacctcac gggcaattgt tggaaggttg tagcagcaaa tgatcttttt
    18721 tcttatagaa caatagaaaa cccaacttct attacaatca aatctttcga caaaaaagaa
    18781 tatccaatca acagagaaat taaacctatg tatagcccag ctgtaaatgt tgtatttgac
    18841 aatggtttcg atttttctat tccaacaatt tcagatactt caagaattat taaatacact
    18901 tcaatatttg ttggtatggg gatgggctat caccccaatc ccgaatttaa gggaatacct
    18961 ttgaagtcgc ttatgaaaaa acctaacaat ttatttgata gggaatggat aatgaatgga
    19021 ttagtttgct tttcatcaat tgacggatac agagcaattt atagctatag tgagttgttt
    19081 aatagaagcg accaagtaat tcctatcctt tctattccag agaatgttga agatggtgga
    19141 tattatagaa tatacttgcc aactgacttt tttgcagata ggagtgttaa atccttgaaa
    19201 gaaatctatt tctttaagga ttaataatca atatagtata gttaacaaac cggatactcc
    19261 ttttattatt cttagtgaat cgagggtttt ctatttttat tgttttgaga tgcttcgcat
    19321 cgctcaactt gataaatatc cttaactaaa cgacacttga aataatttgt gataaagtaa
    19381 acaggttaaa tttaaacttt aagaaaaaac gctagggttc aacaagggat gtaaccattt
    19441 tgggttaaat ggattaggca agcgggttgg cacgcaggag cttcttaacg taggatatat
    19501 cttaagcccc gcacctccaa actggtcacc ccccccagcc gttaaatccc tcccttactc
    19561 cttgactttt tcttcatagc tctgcgttaa aacacactaa ataaaatccc ccggactgtt
    19621 tatgtaccgg gggatttggt taggtttggt taggtgagtg tattactgta atggttttac
    19681 cacttcaatg agctcgggta aatccatgcc aattttcttg aggtgctcaa tggtgggcac
    19741 gccattctta ttccagccac ggcgctcgta aacggcatcg agcagtttct cgtagcgatc
    19801 ctcgcggtac ttgcgtaggg cggccatctt ctcctcaagg gtcatcttgg taggatcaac
    19861 tcccacctgc tccttaagct gcttatcgta gcgctcctcg cggctgaggt actcctcctt
    19921 ggtaacagga ccagcagcgc ggtagggttg agcgtcgtac ttgcgggtac catagccccg
    19981 gcgaatgttg aagatgcgct ggaagttgta aacgcgttcg ctctggcgga taagctcatg
    20041 cttatcgata ttgctgccgg ttacggcgtt gtatatggta acgtagttgt caacgtgctc
    20101 gggaacctta gctggctcat cggtctcggc gttgttttcg ggctcaacat cgttccaggg
    20161 gagcttgcaa agaccctgaa gcccaaacca tgtacggaac atggggaagt agtgcagcgc
    20221 ctcggcctta tcctcaaagg ttggtatgtg gttgttaacc atatccatga aaatgagcca
    20281 agcctcgtcg tgttgtgggc ccttgttggt catggcgtag ccaccctgct gtgccaacga
    20341 ttcctttgaa acgtactggc tgtactccag gcctttgtta accatggcaa tgtcgttcag
    20401 gaatttctca tcggcaccgt actccttaac aaagtgttcc ttaagctttt taacgcccat
    20461 cccggcaagt ttaccaaagc cttcgcctcg ggcaacctgg tgcagcagct caagggcagc
    20521 atccttgttg ccaaacctga gttccagacc accggtacgc tccttattaa gaataccgcg
    20581 ctcatagcac tccatgatga atgccatggt ggtgccccag gtgatggtgc aaatgccgta
    20641 ggtatcgcag tagaagttgg cctcaataac gtattcagga tcaaaaattc cgcagttgga
    20701 gcctaatccg gcagcggttt cgtactcggg accatcaaca atcactttct gtcccttgta
    20761 ggggccggtt ttaatctcga acttatccac acccttggcg cagctcatgt tgcaaccaat
    20821 ccagcaacca tcgggaacac cctgggtgaa gtagctcttc catacatcgg aatgaatctt
    20881 gtgagcatcc ttatgcgagc cgtactggaa gttgtgggtt gggagcaggt cgtaatcgtt
    20941 catgatttca accaggtggg cggtaccctt gctgcgcatc tggcactgct tatcgtcgag
    21001 ctcacgtatt tcgcggttaa agcggcggcc acgctcagca atggcttcaa ggtcggccac
    21061 gttgttaagg ttacccttaa cgccgggaat cttgcaaaca atagccttaa tcttcttatc
    21121 gcggaaaacg gtaccaatac caccacggcc ggcttgcttt aggcggatgc acttacgttt
    21181 gggatcgtag aaggtgaagt ttagcatgcc aataaggcta tggtcggcag cagcgccagt
    21241 tgatactata ccgatattct tcttatcggc ttcgtcgttg gcaaacatct cgcttagcac
    21301 ctcgccaaga atatggctgt cgagcggctc ggcgggagct tcaaaaatct caaccttatg
    21361 ctcaacgccg tcgatgtaaa tgatcacatc gtttttagcc tttccctgaa tctcaagggc
    21421 atcaaaacct gagaacttga ggaaaggacc aaagaaacca cctacgtttg agtcgataac
    21481 aatatcggtt tggggcgaga tggtacaaac tatcgattta ccagtaccag agtactgggt
    21541 gataccgcca atgggacccg aagagataac aatctcgttc tcggggtcgt tccatttggt
    21601 attgggtttg gttgcatccc acaaaaggcg caggtcgtaa cccttaccgc caatgaattt
    21661 ctccttcatg attgggggta cgggtttttc cttaacctcg ttggttccaa cgtttatgta
    21721 caaggtttta tcggtgtagc cgcggtaaag cggtttccac tcgtacttcc actggttcag
    21781 catcttgtgc tgcttagcca tttccttaac gtccatagtg tttatgtttt ggtttaattt
    21841 ttagttctgt tatagtattc gcagctataa aagtacccgt aaagttatca ataaaaaatg
    21901 acttctatca atatgaaatc gtttgcatat tggaatattg ccgcgaaagc catgtgcaaa
    21961 ctatcatttt tatagcgatt tttcaatttt ttattcgaat tgttattcct ctgaaattct
    22021 atcacttaaa aaattattcg aaatttaaca caaattagtc gaatatttta ttcaccgttt
    22081 aagcctatgt tgtatttttg gaacgttaaa ctaaaatgtt cgaaagttta aatactctaa
    22141 atatgaacca gttagaagat agccacaagg gaatgcgcga gaatattgtt agcgcggcta
    22201 ccgaagtttt cagccggtat ggctttaaga aaacatcgat ggaggatatt gcccgggcac
    22261 tacgaatggg taaaagctcc atttactact actttaaggg taaggaggag atttttcagg
    22321 cagtggttga ccgggaagcc aacctgctta gggttaaggt taaggagata cttgagagtg
    22381 ggctgcccgt taccgaaaag cttcgctcct acgttaagat gcgaatggat ttaatcaagc
    22441 agctatcaaa ctacatggaa atccttaaga acgacgacct aatgaacctg gagctaaccg
    22501 aaaagttcag gaaaaagtac gacgacgagg agataaccat tgtaaaacag atgcttgagg
    22561 agggtataaa taggaatgag ctcaaggtaa aggatttaaa cctatcggca ttggccatag
    22621 ttacggccat gaaaggattg gagattccac tggttacctc aaccatgggt gtggagagcc
    22681 ttaatatggt tattgacgat atgctcgata ttctttttta cggaattgta aaacgttaac
    22741 atacaacagc tatgaagatt ttaagattgg tttctttttt cggggcatgg ctaattggta
    22801 ttggctatgt ttcgggtcag caagttctaa ctctggaaca gtgccggcaa atggcgctgg
    22861 agcataacca aaaggtttta attgccgatg agcatgttga agcggcaggt gcattgaagc
    22921 gttctgcaca aacacaattc ctgccaagta tcagcgcaaa cgggctttac acccgaacca
    22981 acaaaaagtt cagcctgctc gataaggatt tgatgcttcc ggttataccc tacacggcca
    23041 ttgacccggt aaccggcggg cttaacagcg caatccttag cccaaccctc cccgatggaa
    23101 ccccaaaccc aaactttaac ccatcggttt ttggcagcac ctttgccatt gaccccagta
    23161 ccggacaacc ctataccgat agcgatggga acccgctctt tcagcgatat acctggctcc
    23221 caaaggataa ggctgagttt ggcaataaga atgtttacgt ggcaggtgtt aatctcaccc
    23281 agcccatctt tacaggtgga aaaatcaggg aaacctatcg cattgcaaag tatggcgaaa
    23341 agttagcccg ggcgcaaagc gaagctgaac gtaccgaggt actatacaaa acagaggaat
    23401 cgtactggcg agttgcagca gtggctgaaa aggttaagct ggttcaggct tacattgccc
    23461 tgctcgataa actcaatacc gacctggaga actactatgc tgagggcatt atcataaaga
    23521 atgacctgct taa
//
LOCUS       DAOSAL010000037        23512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_1560_length_23512_cov_49.093192, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000037 DAOSAL010000000
VERSION     DAOSAL010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 23512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23512
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1560_length_23512_cov_49.093192"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            736..918
                     /locus_tag="PK049_06610"
     CDS             736..918
                     /locus_tag="PK049_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61266.1"
                     /translation="MGTPWVELPRDTIRHACGVAHLVSLTMPTATFHPRPIFGSGFYA
                     SSCFFLSIPYQGKRLG"
     gene            1508..2512
                     /gene="asnA"
                     /locus_tag="PK049_06615"
     CDS             1508..2512
                     /gene="asnA"
                     /locus_tag="PK049_06615"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018967810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HOK61267.1"
                     /translation="MESKVFETLTGDICRSVETEDAITLIKETFPKLLAKKLNLRRVT
                     APLIVEAGLGINDDLNGIEKPVSFTANSIGKRIEIVQSLAKWKRLTLARLELPSGSGI
                     YTDMNALRPDETPDAIHSVYVDQWDWEKRIDTSERGVEYFRKTVNAIYEAICEMEMVV
                     ATHVDGITPELPKSIHFVHSAELEAMYPTLSSRERENVICKQFGAVCIEGIGYPLSDG
                     KPSDGRAPDYDDWSTLRPDGYRGLNGDILVWHKPLGIALELSSMGIRVDSAALKLQLQ
                     MQGLEHRSKLHYHSLLLNGKLPQSIGGGIGQSRLCMFLLRKVHIAQVQASVWPEGQGL
                     "
     gene            2992..4011
                     /locus_tag="PK049_06620"
     CDS             2992..4011
                     /locus_tag="PK049_06620"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HOK61268.1"
                     /translation="MQNLDWKNLPFGYVKTKYNVRCYYKDGKWSSLEVSDSEYINIHM
                     AATALHYGQEAFEGMKAYRGKDGKIRLFRWDENAQRLQHSAEGILMAPVPKELFYEAV
                     VKAVKLNEEFVPPFGTGASLYIRPLLFGSGAEVGVRPAKEYMFVVFVTPVGPYFKEGF
                     NPVKIAIVRDSDRAAPLGTGTFKVGGNYAASLRGVEKARKAGYSSPMYLDAKEKKYID
                     EIGAANFFGIKNNTYITPKSTSILGSITNKSIIQLAEDMGLKVERRPVPVEELETFEE
                     VGACGTAAIISPIGEIKDLETGKVYTFCKDGKPGQISTKLYETLVGIQYGELEDKHGW
                     VTVIE"
     assembly_gap    4030..4129
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4432..7305
                     /locus_tag="PK049_06625"
     CDS             4432..7305
                     /locus_tag="PK049_06625"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOK61269.1"
                     /translation="MKFLKKIIAFALIAIALQSFAQTQTIRQFTLPNGLTVILDEQHD
                     QPEVFGLIAVKTGGKNDPADATGMAHYQEHMLFKGTTTLGTIDWEKEKPHIDRIFQLY
                     DSLGKTKDPEKRALIQKAINEESVEANKFAIPNEMNNLLNEMGSTLINAGTGPDYTLY
                     YNKFPASQVERWLDLYAHRFTEPVFRGFQAELEVVYEEKNLYNDQFQTRLLEEFQKYF
                     FKNHPYGQQTLIGSIEDLKNPSLTKMYEFFKTYYVPNNMALILSGDFNSEEVIPAIEE
                     KFGKWQPRELPEPRKWDEAPFNGREFHEAKLSPIKLAILGYRAPNATDPRKPLLDVTT
                     RLLNNSYSTGLLDKLTLDGKLLAAQAMMMPYYDHGAIIFIVVPKVVGQKLEDAEKLVL
                     SEIEKLKKGEFDESLLEAIKLEIYRNTNTKLENIQNRALTLSEAFTQGKSIDEALTYP
                     EKVKSITKADIVAIANEVFGPNYLAFYSKMGFPKKEKIAKPDFKPLLSNTKARSAYAQ
                     HFDSLPIRTPNFKFIDFEKDLKVVELAGGHRLQCVENPKNDIFSLSIEFKVGKASLPL
                     LGFATQGMALAGAGEYDVNALKTEFAKLGSSYSIWANENKTTITINGIESNLEPTLKL
                     VGLLLSDPKLEQSKIKTIVANEKATRKMERSEPDNVADALVEYGLYGDESYYTKRLSM
                     SSVKKLKAQELVDAFKQAAGYTATISYCGNTSADKISDIIASYLPLSSSPVVDTTPLD
                     KPAKQYTENTILFVNKPKARQSKMFVFINGKPFEIGDAAYIDAFNDYFGGGFSGLMLQ
                     EIREYRSLAYATGGSFRYPLEKGKPVNFIGYVGTQSDKTLIAMETLDTLIRQMPAKPE
                     RIEMIKSHLELSAQTSRPNFRNIASTVDVWRKRGFPVDPLIIKLPIYRGLTWENIDEF
                     YKDRMKNNPVVYMIVGDEKNIDMKSLAKYGKLVKISEKTLFSK"
     gene            7394..8968
                     /locus_tag="PK049_06630"
     CDS             7394..8968
                     /locus_tag="PK049_06630"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF006006.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HOK61270.1"
                     /translation="MKIKIILIIAVAATLVSCQRQGSSDIDSTAYLEEITIKQLQDGY
                     REKRFTVEKVVNDYLKRIELLDKEGPCLNSVIAINPDALKIARELDKELAQGKVRGPL
                     HGIPVILKDNIDTRDSMPTTAGATVLRNSFVGRDSWIAAKLREAGAVIIAKANLSEWA
                     NFRASRSSSGWSGVGGQTRNPYELDRNPCGSSSGSGVAVSANLCAFAIGTETDGSIMC
                     PSNNNGIVGLKPTVGLISRSGIIPISFTQDTPGPMCRSVSDVAYCLGAMVGPDPNDPK
                     TLDPNARFYNDYTQFLRLDGLMGKRIGFITNASGFHHKVDTLMSKAISDLRRFGAEVF
                     EVSLPIPQEVNSAEFQVLLYEFKDGLNRYFAGLGEKAPIKSLEELISFNRTDTIELRH
                     FDQHLLELAQGKGDLNSPEYIKALETMQKGIRQNGIDKVMDENKLDLLIIPTGSPAWK
                     TDLILGDNYIGGNTSYAAMAGYPSITVPMGNIDGLPVGISFIGRAWCESTLIEAAYSY
                     EQGTKHRIVPKFKASK"
     gene            complement(9129..9374)
                     /locus_tag="PK049_06635"
     CDS             complement(9129..9374)
                     /locus_tag="PK049_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61271.1"
                     /translation="MGAPGVELPRDTIRHACGVTHVVSLTKPTATFHPRPIAESGFYA
                     TTCFFRYIPPQGIGLGEKPFSTFNLDAPSKSPLKGRL"
     gene            10045..10944
                     /locus_tag="PK049_06640"
     CDS             10045..10944
                     /locus_tag="PK049_06640"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HOK61272.1"
                     /translation="MQKYLLALIAVLAFSFVHGQQAGGKKRQPKGPYPNDWVLVEKGG
                     SFGFISSTGKEIIKPQYTRIHPFDEFQRGWAMVEKDGFFGFISAEGKEIIKPQYTKIY
                     PFGDFNRGWAMVEVNGKFGFISVEGREIIKPIYSKIYPIGEFYKNLAMVEKDGLIGFI
                     SLDGREVLKPQYQKVHPFGEYQKDWALVEKDGFLGFINGEGREIVKPQYSKISQFNEY
                     QKGWALVQRDNYMGFINSEGKEVIKPQYTQIMPFDQVQRGWALVERDGLWGFISSEGR
                     EVVKPKYEKISFNDSTAFQKTRP"
     gene            11013..11198
                     /locus_tag="PK049_06645"
     CDS             11013..11198
                     /locus_tag="PK049_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61273.1"
                     /translation="MGRFRIEAKWALIFSLMTLAWMLLEKVSGLHGKYIDYQYYLTNL
                     FAIPAIAVMVQISFMAT"
     assembly_gap    11231..11330
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11331..11637
                     /locus_tag="PK049_06650"
     CDS             <11331..11637
                     /locus_tag="PK049_06650"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HOK61274.1"
                     /translation="NFFHGNMSYLNGLITGIVLSLFIAILSPLTQWIISYVISPEYFP
                     NVIKRSVELGYYKSVAEAEAFFNYKNFAIQSAIGALVMGILTTAIAMIFIRTKGSKK"
     regulatory      11795..11898
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            12149..13453
                     /locus_tag="PK049_06655"
     CDS             12149..13453
                     /locus_tag="PK049_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HOK61275.1"
                     /translation="MNNTYHFDTLQVHAGQGVDTNTLSRAVPIYQTTSYLFRDSEHGA
                     NLFALKEFGHIYTRLSNPTTDVLEQRLAALEGGKSAVVVSSGHAAQFIALTNIMESGD
                     NFVSSPYLYGGSHNQFKVTLKRLGIEARLAKSLKPEDFECLIDSKTKAIYVETIGNPS
                     FNIPDFKALTTLANQHQIPLIVDNTFAGAGYLCRPIEWGANIVVESITKWAGGHGTSM
                     GGVIVDAGNFNWGNGKFPQFSEPSEGYHGLVFWTAFGEDSPHGNIAFAIRARVEGLRD
                     FGPAISPFNAFQILQGIETLSLRMERHCSNALALAKWLKTNRHVERVCYPGLPDDPNH
                     IQAKKYLKNGFGGVLTFTLKGNLEQTQTFVDSLKLVSHLANVGDAKTLIIQPAATTHQ
                     QLSEEERLAAGVLPNLLRVSVGIEHINDIIADFEQAFQKTFS"
     gene            13504..14523
                     /gene="metX"
                     /locus_tag="PK049_06660"
     CDS             13504..14523
                     /gene="metX"
                     /locus_tag="PK049_06660"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004414 - homoserine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HOK61276.1"
                     /translation="MKVTEGNLELKTLQLECGEVLYDVNIRYHILGDIQKNPERVIWI
                     CHAFTANSNPADWWPEMVGQGRLFDPEKYTVVCANIIGSCYGSTGPMSVNPKTGKPYL
                     RSFPLVTFRDVVTAHQILKEYLGIDKIWVLTGGSIGGFQAMEWECMYPGTTENLILIA
                     TAAQASPWVKAFSQTQRLAILADGTFDGANPNGGKAGLKAARSIALLSYRGRSSYNLT
                     QQDSDPNQLSDFKACTYQDYQGDKLVKRFDAYSYFALSLMLDSHNMGRGRVSNEAALN
                     SIKARTLVVGIDSDILFPVEEQHFIADHIPSAVYREIHSQFCHDGFLIEYQQLTSIIS
                     EFINL"
     gene            14537..15853
                     /locus_tag="PK049_06665"
     CDS             14537..15853
                     /locus_tag="PK049_06665"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOK61277.1"
                     /translation="METLTITNTRKNSKSNGVNQQVIGLFGLGNVGNALYHVIAEIPK
                     SKAYIKRICVKQRHKNREVPHELITYNAADIINDEEINLIVELIDNADEAYHIVKSAL
                     LRGKSVVSGNKTMLAHHLPELIAIQHETGAALLYDASACGSIPVIRNLEEYYDNDLLK
                     SIRGILNGSTNYILSNVFSQGVDYHWALKQAQELGFAESNPDFDVLGFDALYKLIILA
                     VHGFGTYVHPSDAFCYGINNLKAYDIQFAREKGKKFKLVAQLQKLNADYFTLFVIPTL
                     VEPSDYIYNVENEFNGVVVEGQYYDRQFMFGKGAGGYPTGSAVLSDITARFHNYRYEY
                     KKQKYFTPPAYSTDVELELYIRYHNLTDVSIFGERKVTEQHFATDYRWEIAWVKLNEL
                     IRVKELLPKLNLFLAFTGNFRFNPIDKALSSKVTCADDYQPIVDAF"
     gene            15865..19536
                     /gene="metH"
                     /locus_tag="PK049_06670"
     CDS             15865..19536
                     /gene="metH"
                     /locus_tag="PK049_06670"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF007024.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HOK61278.1"
                     /translation="MEKTTVFRELLKSRVLVLDGAMGTMIQRYRLKEEDFRGDILKNH
                     PVNLKGDNDLLNLTRPDVIGQIHRAYLEAGADIIETNTFNSTRISQSDYGLEQMVYEL
                     NLAGARLARSIADEFSNANPQKPRFVAGSIGPTNRTASMSPDVERPGYRAVTFDQLVN
                     SYTEQAETLIDGGVDILLIETIFDTLNAKAALFAISEILERRGIDIPVMVSATIADNG
                     GRLLTGQTLEAFVCSVSHFPLLSIGLNCAFGAEQMYPFVEELSAITPFFVSAHPNAGL
                     PNQFGEYDQSAQEMAEIVEQMLANGLVNIVGGCCGTTPKHIELVANLAQRYKPRIIPE
                     IPRHTRLSGLELLEIRPESNFINIGERTNVAGSKKFARLIAEGKYAEALSVAREQVEG
                     GAQAIDICMDDALIDAPRAMTEFLNLIASEPDVARIPVMIDSSRFDVIEAGLKCTQGK
                     SLVNSISLKEGETEFARRAKVIKRYGAAVVVMLFDESGQAVTTEHRCRVAERSYRILT
                     EKVGFSPEDIIIDPNILAIGTGMVEHSTQAVSFIETIKWIKQNLPYAKVSGGVSNLSF
                     SFRGNNLVREAIHSVFLYHAIQAGMDMGIVNPSLLMVYTDIEPNLLKLTEDLVLNRRR
                     DATERLLAYASKLEETDLQHHKTDDWRLLPVEQRLKHSLIKGTDEFIRVDVEEAYNKL
                     KSPISVIEGPLMDGMREVGNLFGTGRMFLPQVVKSARVMKQAVKVLEPYLEAQAMDSH
                     RTEQKKIVLATVKGDVHDIGKNIVGVVLACNGYSIIDLGVMVPAERIVDEVKASKADI
                     VGLSGLITPSLDEMVHVVKELKRNGINIPVLIGGATTSELHTALKIAPEYSGAVVHVR
                     DASQASGIVASLLSTDKSASTINAYQKRYQQQRESYSGSKTVLVPIEQARKNSLKLNW
                     DSYKPVRPKQIGINTLSNIDLNELIHYIDWTFFFRAWGLKGRYPDILTDPVHGSEAQK
                     LKAEALAMLKQISEQKLLTPEGVYGIFPAASSGDDVIVYTDETRTSPLTHLPFLRNQE
                     QKPEGEPNMCLSDFIAPANSGINDWIGCFAVTTGSTTSTLIEEYRKNGNDFAAIMIQI
                     LADRLAEAFAEWLHLKIRKDFWGYSPNESLTVEQLLQEKYVGIRPAPGYPACPDHRGK
                     QWIFQLLDIPRNASISLTENMAMNPLASVAGFYFASSASKYFSVGKIDNDQLADYAKR
                     IGVSTTEAARLFPTFLANQ"
     gene            19548..20501
                     /gene="metF"
                     /locus_tag="PK049_06675"
     CDS             19548..20501
                     /gene="metF"
                     /locus_tag="PK049_06675"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HOK61279.1"
                     /translation="MKVIDILNNSKSTLFSFELLPPLKGHNIETIFATIDKLLPYNPT
                     YINITYHREEVVYMSNENGIQVPVKVRRRPGTIGLAAAIQQRYAIDVVPHIICGGFDA
                     HETEDALIDLDFLGIQNILAVRGDADKITGRFEPRAGGHRYAIDLVKQVTDLNRGVYL
                     EKLMEKPKPTNFCIGVAGYPEKHPESPNCESDIHYLKKKVEAGASYIVTQMFFDNKLY
                     FNFVDKCRKAGIAVPIVPGLKPISIKEHLSILPRVFDVHIPQALVLELEKCKTNNQVA
                     QLGVEWAIEQSRELKSSGVKAIHFYTMGKDDNIAQIAKAVF"
     gene            20815..23151
                     /locus_tag="PK049_06680"
     CDS             20815..23151
                     /locus_tag="PK049_06680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOK61280.1"
                     /translation="MAQKKVGEKRFEGSFEYDGELKYRLIFEKSPIGLFQYNTDAVIT
                     ECNDRFVEILKSKRELLVGLNMHKINDKSVLPAILDALDGKNGYYEGPYTTTTSNAQI
                     YVLLKAVPLTNPKTGQVVGAMGIVEDISDLYRTQEELTIKEERFRILSSLTSDSASIL
                     TIQPDGSFKREWLSSALMVQLGYKPEDVDSFEKWATIVHPDDIDVFKRAIGTITQKGE
                     KVTLEFRIKAKNGKVHWIENTVYPEFDSSGKPFRLISAVKDITAKKEQEQELQQQRNL
                     LKSIVDNAPLGIWVVNPDGSYPIINPWFSQNIGYGTSQFSMTEEEVAQCRRSDMQALN
                     SDGPIETTEEVTFTDGKKHILQIFKQKLTSTTGETIGVLGIANDISERTQYEKALVEA
                     LEKAEESDRLKTAFLANMSHEIRTPLNGVIGFAKFLRNFPDTTLQEREKFLGIISTSA
                     DHLLTLINDIIDISKIDVGQLTINPEAVNINELLNEIYTFFYNANPDLPKREIAFNYT
                     TSLPDSKAMVNADRMRLRQVLTNLINNALKFTDKGKVEFGYNINSETNEFEFFVSDTG
                     IGIAKDKQEIIFQRFRQAYTDITKQYGGTGLGLAICKSLVELMGGRIWVESETGKGST
                     FRFTLPINAPTTQNRGKVYGYELQELKNLLKGKSILIAEDDPNCMYYLKTVLKDFDIN
                     ILEADNGLLATEIQYQHPEIAAILMDIKMPVMNGYDAIKRIRSTNAQVPIIVQTAHTF
                     SNERAISIALGCNQFITKPIDSNSLYQSLYSVLKESRR"
BASE COUNT         7070 a         5123 c         5161 g         5958 t
ORIGIN      
        1 gaggagttgg ttgaagggat agcaaggtta aaggataaag gataaaggat aaagggtaaa
       61 gcgtgaaccg tgaaccgtga accgtgaacc gttaaacggg atagaacatt atgtactgcc
      121 tagtgtaatc acgaaggata aaggattaag tttagaggag gttaatacac aaggttcttt
      181 ttacgaaaaa cgattcacga acaacgctat ttgtcattcc gagtcccgat tatatcggga
      241 tagactatgt cgaggaatct cattaatgaa gagttgtagg tgcaaagaga tgcttcggcg
      301 agctcagcat gacaaacgta tcatgaaaca ggcaacagca agtatcacag agttacacag
      361 tgcaacacgg tgtttaacag aatggcattc acgaacaacg agcaacgttt cacgaacaac
      421 gctatttgtc attccgagct agtagaggaa tctcgattta tttaagattt aaaatttaag
      481 aactcactag ttaacacacg tctttcgaca cgcacattat tcgccctaaa agggcaaatc
      541 acattagccc agggttttaa ccctgggtat tgtggtattc aaaattatag gcgatgcacg
      601 caatactttt tccaaagagt aatggtgatg cgttgcatcg caaaagtaaa gcattgcaaa
      661 gagcggtttg catgttcttt cttgtcttga tacaagaaag aaccaaagaa aatcaaggct
      721 gaaaagcccg acccgatggg taccccgtgg gtggaacttc cacgcgatac tattcgccac
      781 gcctgcggcg tggctcattt ggtatcgctt acaatgccca ccgccacgtt ccacccccga
      841 cccattttcg ggtcaggctt ttatgcctct tcttgcttct tcctctccat tccttatcag
      901 ggaaaaaggc ttgggtgagg tcaatttcac gaacaacgaa caacgtttca caaacaacga
      961 acaatgcttc cagccccgta ggggcttaat atttgtaacc caataactac tctcataaat
     1021 tgcgatgcac gcaatgcata tgccaaagag gtaaattgaa acgttgcatc gcaacagata
     1081 agcagtgcaa agagccattt tcacgttctt tcattccttg acacaagaga gaactaaaga
     1141 aaatccagag taaaaatttg catttgttat attctttttc gatattgcac gtgtaaatgt
     1201 gaaattaaaa tgattcgata ctccaaagcc aataataatt ccaatttcaa caataacctg
     1261 ttgaaacggg aaggttttgg cgtttggtga gcaagataca atactagttc aaggccttcc
     1321 taaaaaaaca ggaaggcctt ttttgtttaa cacattccat atgctgcaaa gagtaaataa
     1381 taacaacaat aatcgtccgc caccccccgg gaatggttat gctttaccct gcccttcccg
     1441 gtaattccgc gaagggcatt cttttttcaa acttcaaact atcaacacta accaaaataa
     1501 tataaccatg gaatcaaaag tttttgaaac acttacaggc gacatttgcc gttcggttga
     1561 aaccgaggac gctatcaccc taatcaagga gacctttcca aagctgctgg caaaaaagct
     1621 aaacctgagg cgggtaaccg caccgcttat agttgaggct ggtcttggca ttaacgacga
     1681 tttaaacgga attgaaaagc ccgtaagttt taccgccaat tccattggca aacgaattga
     1741 aattgttcaa tctcttgcga aatggaagcg attaacactt gcccgtttgg aattacccag
     1801 tggtagcgga atatataccg atatgaatgc actaaggccc gatgaaaccc ccgatgcaat
     1861 tcactcagtt tacgttgacc agtgggactg ggaaaaacgt atcgatactt cggaaagagg
     1921 cgtggaatac ttccgtaaaa cagttaatgc tatttatgaa gccatctgcg aaatggaaat
     1981 ggtggttgcc actcacgtcg atgggattac tcccgaacta cccaagagca ttcattttgt
     2041 tcattcggca gaattggagg caatgtaccc aactctatcg tcgcgtgaga gagagaatgt
     2101 tatatgcaaa cagtttggag cagtttgcat cgaagggatt ggatacccgt tgtccgatgg
     2161 taaaccatcg gatggaagag cccccgatta cgatgattgg tcaacactcc gccccgatgg
     2221 atacagaggt ctaaatggcg atattcttgt ttggcacaag ccactaggaa tagcccttga
     2281 actctcttcg atgggaatta gggtggacag tgcagcccta aagttacagc tgcagatgca
     2341 aggcttagag catcgaagta aattgcacta ccatagcttg ctactaaacg gtaaacttcc
     2401 acaaagcatt gggggaggca tagggcaatc gcgactctgc atgttcttac tccgcaaggt
     2461 acatattgca caggtgcaag catcggtttg gccggagggg caaggattat gacgaaggat
     2521 aaaggataaa ggataaagga taaagggtaa agcgtgaacc gttaaacgtg agccgttaaa
     2581 tgggattgaa caataagcga aaccccctgt tatcaagaag gataatggat aaaatttaga
     2641 agaggttaat acaaaaggtt ctttttacga ataacgaatc acgaacaacg ctacttgtca
     2701 ttccgagtca tatcctaaaa aataacacac atgattttgt tataaaaaac aggcattgct
     2761 ggtttcaatt attcctaata cactcctacc caaattaacg cgcgctgaat gattgcgtgc
     2821 gtttttttta cataaccgaa atgtatcttt caaattatta ccgttttacg agtcaaactt
     2881 acattttaaa cctttttatt gtaaaatatc taaaaatatg aagcgattaa aacagatttg
     2941 ctttaacttt gtcatatcac aatatgtcca attcattaaa ctgttggaat catgcaaaat
     3001 ttagactgga agaatcttcc gtttggttac gttaaaacca agtataacgt tcgatgctat
     3061 tacaaggatg gcaaatggag cagccttgag gtatcggatt cagaatatat taacattcac
     3121 atggccgcta cggccctgca ttacggccaa gaggcctttg agggcatgaa ggcttaccgt
     3181 ggcaaagatg ggaaaattcg tcttttcaga tgggatgaga acgcccagcg gcttcaacac
     3241 tcagctgaag ggattttaat ggcacctgtg cccaaagaac ttttttacga agccgtggtt
     3301 aaagccgtaa agctgaacga ggagtttgta ccgccattcg gaactggggc atcgctttac
     3361 attaggcctt tgctatttgg ctctggtgcc gaggttggcg ttcggcctgc taaggagtac
     3421 atgtttgttg tgtttgttac tcctgtagga ccatacttta aggaaggctt caaccctgtt
     3481 aaaattgcca ttgttcgcga tagcgaccga gcagccccac ttggtactgg tacctttaag
     3541 gtaggtggaa attacgcagc cagtcttcgg ggggtagaga aagcccgcaa agcaggatac
     3601 agttcaccaa tgtatcttga tgcaaaggag aagaagtata ttgatgaaat aggtgctgcc
     3661 aatttctttg gtatcaagaa caacacctac atcaccccaa aatcaacatc aatactaggc
     3721 tccattacta acaagagcat tattcagctt gccgaggata tgggcttgaa ggttgaacgc
     3781 cgccctgttc ctgttgagga acttgaaact tttgaagagg ttggagcatg cggaaccgct
     3841 gcaatcattt caccaattgg ggagatcaag gatttggaaa cagggaaggt ttacacattc
     3901 tgcaaggatg gtaagcccgg ccaaatctca accaaacttt acgaaaccct tgttggaatt
     3961 caatacggcg agcttgaaga taagcatgga tgggtaaccg tgattgagta agatagataa
     4021 ggataaaggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt gaagataagc
     4141 atggatgggt aaccgtgatt gagtaagata gataaggata aaggataaag gataaagggt
     4201 aaaaggatgt cttttgtgtg acaatagaaa cattaaaacc tagataactt attttaagca
     4261 aaaccggatt aatagttcat ctctgaactt ttccggtttt gtttttttgg gggaataata
     4321 acgccgttaa aaagcaaaat cttaaacagt cagtcagcta agtaagattt ttttatcttt
     4381 acaaataaca aaatccatag gctaaccaat aaacgtaaac catttaaaac tatgaaattt
     4441 ttaaaaaaaa ttattgcatt tgcacttatt gccattgcgt tacagtcatt tgctcaaacc
     4501 caaacaatca ggcaatttac cttgcccaac ggattaaccg ttatacttga tgaacaacac
     4561 gaccaacccg aagtgtttgg cctgattgca gtgaaaactg gcggcaagaa cgaccccgcc
     4621 gatgctaccg gcatggccca ctaccaggaa catatgcttt ttaaggggac aaccacgctg
     4681 ggaaccattg actgggaaaa ggagaaacca catatcgaca ggatatttca gctttacgat
     4741 tcgcttggca aaaccaaaga tcccgaaaaa agagcattaa ttcagaaagc gataaacgaa
     4801 gagtccgttg aggcaaacaa attcgccata cccaacgaga tgaataacct gctgaatgaa
     4861 atgggttcaa ccctaattaa tgccggaaca ggtcctgatt acaccctata ctacaacaaa
     4921 tttccggcaa gccaggtgga acgttggctc gacctatacg cccatcggtt cactgaaccc
     4981 gtatttagag gttttcaggc agaactcgaa gtggtttatg aggagaaaaa cctttacaac
     5041 gatcagttcc agacccgttt actagaggaa ttccagaaat attttttcaa aaatcaccct
     5101 tatggacaac aaaccctgat aggaagcatt gaggacttaa agaatccttc gcttaccaaa
     5161 atgtatgagt ttttcaaaac atactatgtg cccaacaaca tggccctaat cctatcgggc
     5221 gattttaatt ccgaagaagt tattcccgcc attgaagaaa agtttggcaa atggcagccc
     5281 cgtgaactgc ctgaaccccg aaaatgggat gaggctccct ttaacggacg cgaattccac
     5341 gaggcgaaac ttagtcctat aaagcttgca atacttggtt atcgcgctcc aaacgctacc
     5401 gatccccgta agcccttgct cgatgttact acccgattac ttaacaacag ctactctacc
     5461 ggtttactgg acaagctaac gctcgatgga aaattgcttg cagctcaagc catgatgatg
     5521 ccatactacg accatggagc aataatcttt attgttgttc ccaaggtggt tggccaaaaa
     5581 cttgaagatg ccgaaaaatt ggttctctct gaaattgaaa agcttaaaaa aggagaattt
     5641 gacgagagcc tgttggaagc aataaagctt gaaatttacc gtaacacaaa cacaaaactc
     5701 gaaaacattc agaacagagc attaaccctc agcgaagcct tcacccaagg taaaagtatt
     5761 gatgaggcac tcacctaccc cgaaaaggta aaatcgataa ccaaagccga tattgtagct
     5821 attgcaaacg aggtttttgg ccctaactac cttgcctttt actccaaaat gggcttcccc
     5881 aaaaaggaaa agatagccaa gcccgacttc aagcccctac tctcaaatac aaaagcccga
     5941 agcgcttatg cccagcattt cgactcgctc cctattcgca cccctaactt taaattcatt
     6001 gattttgaaa aagatttgaa agtggttgaa cttgccggtg ggcataggct ccaatgcgtt
     6061 gaaaacccca agaacgatat tttctcattg agtattgagt ttaaagtagg taaagcttca
     6121 ctgccattgc ttggatttgc tacacagggt atggctttag ccggagcagg cgaatacgat
     6181 gtaaacgcac tgaaaactga atttgccaag ttaggctcat cgtatagcat ctgggctaat
     6241 gaaaataaaa ctactataac cattaatggt attgaaagca acctggaacc cacactcaaa
     6301 cttgttggct tactgcttag cgaccccaaa cttgaacaat caaaaattaa aacaattgtg
     6361 gccaacgaga aggcaacgcg taagatggaa cgctccgaac ccgacaacgt ggccgatgca
     6421 ctagttgaat atgggttata tggcgatgaa tcctactata ccaaacggtt atccatgagc
     6481 agtgtaaaaa agcttaaagc ccaagagctg gttgatgctt ttaagcaagc ggctggttat
     6541 actgccacca taagctattg cggtaatacc agtgccgata aaatttccga tataatagcc
     6601 agctacttac cactctcctc aagcccagtg gttgacacaa cacccctcga taaacctgct
     6661 aaacagtaca ccgaaaacac catccttttt gtcaataaac caaaggcacg tcaaagcaag
     6721 atgtttgtat ttattaatgg caaacccttt gaaattggtg atgctgcata catcgatgcc
     6781 tttaacgact actttggcgg aggtttttcc ggcttaatgc ttcaggaaat acgcgaatat
     6841 cgctcactgg catacgctac aggtggctcg ttccgctacc cgctagaaaa gggtaaaccg
     6901 gttaacttta ttggttatgt tggaacccag tccgataaaa cgcttattgc catggaaacc
     6961 ctcgatactc ttatcaggca aatgccagca aaacctgaac gtattgagat gattaaaagc
     7021 cacctggagc tatcggcaca aaccagccga ccaaacttcc gcaatattgc atcaactgtt
     7081 gatgtgtgga gaaaaagagg ctttccggtt gatccactaa ttattaaact accaatttac
     7141 cggggtttaa catgggaaaa tattgatgaa ttctacaagg atcgcatgaa aaacaaccct
     7201 gttgtttaca tgattgttgg cgatgaaaag aatatcgaca tgaagagcct tgccaaatac
     7261 ggcaaactag taaaaatcag cgaaaaaaca ctcttcagta agtagttcct attacagtat
     7321 tacaataaag gcagtcccta attgactgcc tttatttttt tcactaaatt tcgattgcca
     7381 aaaacaaaat accatgaaaa tcaaaatcat attaataata gctgttgcag cgacacttgt
     7441 atcgtgccaa aggcaaggct cgtcagatat tgacagcact gcatacctag aagaaattac
     7501 cataaaacag ctgcaggacg gttatcgcga aaagcgcttt accgtggaga aggttgtaaa
     7561 cgactatctg aagcgcattg aactccttga taaagaaggt ccttgcctga actcggttat
     7621 agccatcaac cccgatgcgc taaaaatagc cagggaatta gataaggaat tagcacaagg
     7681 caaggttcgt ggcccacttc atggaatacc tgtaatccta aaggataaca ttgatacacg
     7741 cgactccatg ccaaccactg ccggtgcaac ggttttaagg aactccttcg ttggacgcga
     7801 tagctggatt gctgcaaaac ttcgcgaagc cggtgcagta ataatagcca aagcaaacct
     7861 gagcgaatgg gctaacttta gggcgagtcg ttcgtcgagc ggatggagcg gagttggtgg
     7921 gcaaacccga aacccatacg aacttgacag aaacccctgt gggtcgagtt cggggtcggg
     7981 cgtggccgta tcggctaacc tttgtgcatt tgccattggc actgaaaccg atggttcaat
     8041 catgtgcccg tcgaacaaca acgggattgt gggcctaaag cccacagttg ggcttattag
     8101 ccgcagcggg ataattccca tctcgttcac ccaggacaca ccaggcccca tgtgccgttc
     8161 ggtttccgat gtggcctact gccttggagc aatggttggc cccgatccaa acgacccaaa
     8221 aacacttgac cctaacgcca ggttttataa cgactacaca cagttcctcc gacttgacgg
     8281 gctaatggga aaacgtattg gatttattac caatgcatcg ggctttcacc ataaggttga
     8341 cacgctcatg agcaaggcca taagcgattt gcgccgtttt ggagccgagg tgtttgaggt
     8401 tagcctgccc atacctcaag aagtaaacag tgcagaattt caggtattgc tctatgagtt
     8461 taaggatggg cttaaccgct actttgccgg tttaggcgaa aaagcaccaa ttaagagctt
     8521 agaggagtta atttctttta accgtacgga cactattgaa ctacgccact ttgaccaaca
     8581 tctgcttgaa cttgctcaag gcaaaggtga cctaaactcg cctgaatata taaaggcact
     8641 tgaaaccatg caaaaaggta taaggcagaa tggaattgat aaagtgatgg acgaaaacaa
     8701 acttgaccta ctcataattc caacaggttc gcctgcatgg aaaaccgatt tgattttagg
     8761 tgataattac ataggtggta acacttcgta tgcagctatg gctggttacc caagcattac
     8821 cgttccaatg gggaatattg atggtttgcc tgttggtatc tcgtttattg ggcgcgcctg
     8881 gtgtgagtca acacttattg aagcagcata ctcctacgag caaggaacaa agcacagaat
     8941 agttcctaag ttcaaagcct caaagtaaga cattgaagat ttgttatttc agtaaaaact
     9001 aaattacgcc cctacggggt ttaagacgtt gttcgttgtt agttgctcgt tgttcgtgaa
     9061 attgacctca ccccctcccc tctcctgaaa ggagaggagt gatgcaatta ggaaacctta
     9121 ttgcaaggct aaagtctccc tttcagggga gatttagagg gggcgtctag gttaaatgta
     9181 gaaaacggct tctcccccag ccctattccc tgaggtggaa tgtagcggaa gaagcaagta
     9241 gtggcataaa agcctgactc agcaatgggt cgggggtgga acgtggcggt gggcttagta
     9301 agcgatacca catgagtcac gccgcaggcg tggcgaatag tatcgcgtgg aagttccacc
     9361 cccggggcac ccatcggttc gcgcttttca gccttgatct tctttggttc tttcttgtat
     9421 caagacaagc aagaacatgt aaatcgctct ttgccatgca tttcagttgc gatgcaacgt
     9481 ttcaatattg ctctttgtag tatcatagcg tgcatcgcta tacttgaggg attagccatg
     9541 gtgttacaaa tattacgccc ctacagggct ggaaacggtg ttcgttgttc gtgaaactat
     9601 tgtcaagctg agctcgccga agcatctctt tgtgccttca ttctcttatt tactttttgc
     9661 tctttggaat gttcttctgt tgcgatgcaa caattcagcg ttgctctttg gaaaaagcat
     9721 tgcgtgcatc gcctaaaatt ttgattacca caataccctg ggttaaaacc ctggtctaat
     9781 gtgatttatc cttttagggc ggaataatat gcgtgtcgaa agacgtgtat taacaagtga
     9841 gttcttaaat tttaaatctt aaatctacct ttttaataga ttgtgatgga aattgatttt
     9901 cgaagtttac tgatttcaaa cgcaaaatgc ttatactttg cctacttttt tatcaaccta
     9961 tatgaatttt aagaaaaatc atgtaggttt gtatgatagt gaagcaagta tttacacttg
    10021 tgccctaaca ttaatatgtt ggctatgcaa aaatatctac tcgcactaat tgctgttttg
    10081 gcgtttagtt ttgtacatgg gcagcaagcc gggggtaaaa agcggcaacc taaagggcca
    10141 taccccaacg actgggtttt ggtggaaaag ggtggctcat ttggatttat ttccagcacc
    10201 ggcaaggaga ttattaagcc acaatacaca cgtattcatc ctttcgatga atttcagcgg
    10261 gggtgggcaa tggttgaaaa ggatggattt ttcggattta tctcggccga gggcaaagag
    10321 attatcaaac cccaatacac aaaaatatac ccctttggtg actttaaccg cggatgggca
    10381 atggtagagg tgaacggtaa gtttggattc atatcggttg aaggtcgtga aattatcaag
    10441 ccaatttata gtaaaattta ccctatcggc gagttctaca aaaacctagc catggttgag
    10501 aaagatggat taattggctt tatatcgctc gatggaaggg aagttctaaa accccagtac
    10561 cagaaggtgc atccgtttgg cgaataccaa aaggattggg cattggttga aaaggatggt
    10621 tttcttggct ttatcaatgg cgaagggaga gaaatagtta aaccacaata ctcaaaaatc
    10681 agtcagttta acgagtacca aaagggttgg gcgttagtac agcgcgacaa ctacatggga
    10741 tttattaact ccgagggcaa ggaagtcatt aaaccacagt acactcagat catgcctttt
    10801 gaccaggtgc aaaggggttg ggctcttgtg gaacgcgatg gcctttgggg ttttatttcg
    10861 agcgaaggcc gggaagtggt aaaaccaaag tacgaaaaga ttagttttaa cgattccact
    10921 gccttccaaa agacaaggcc ataatggaat gctttgattc ttgctgggtt tactcaaaga
    10981 acttgttcaa acaaaccata atctagtaaa acatggggag attccgaatt gaagccaagt
    11041 gggcattaat attttcgcta atgaccttgg cttggatgtt gcttgaaaag gtatcggggt
    11101 tacacgggaa atatattgat taccagtatt acctcacaaa tctttttgct attccggcta
    11161 tagctgtaat ggttcaaatt tctttcatgg caacatgagc tacctgaatg gcttaattac
    11221 tggtattgtg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aaatttcttt
    11341 catggcaaca tgagctacct gaatggctta attactggta ttgtgctctc cctattcatt
    11401 gcgattttaa gccccttaac ccagtggata atatcgtatg tgatttcacc cgagtatttc
    11461 ccaaatgtta taaaacgttc tgtggaattg ggttattaca agtctgttgc cgaagctgaa
    11521 gcctttttca actacaaaaa ttttgctatt cagagtgcta ttggtgcatt ggtaatggga
    11581 attttaacca ctgctattgc tatgattttt attaggacaa agggttcgaa aaagtaactt
    11641 tatcccattc ctgatgtcaa cctactatta atagtttacc acatttaatc aattcaaaat
    11701 ttaatataag ttttaccgcc ataaatttca taaatagaat tttttatata tttgttgcca
    11761 tcgaatgcca ttctttttag ataaagaagg gtttgaatta tagagaagag cggagagaac
    11821 aggctcaatg aagctctagc aacctgcccc ccaaaaggca aggtgctaaa acctgacccg
    11881 agagggtaat tataactcta aactctgatg ttatgcaaag taaacatctc aacagtattg
    11941 aattatgcgc aatcagttct tttgcgctaa acaatttact ttttaggctt tcccactctc
    12001 tttcaatgcg aatgcgcccc tgcgcgcaat agctaattca taaaacctat tggttttagc
    12061 ctgcatgccg tttgcggcgg gctaaacaat tgcctgtata taggcaaaaa tctcacactt
    12121 taccatttcc aataaaaata ttgagacaat gaacaataca tatcattttg acaccctgca
    12181 ggttcatgca gggcaagggg tggatacaaa cacgctttcg cgggctgtac ctatatatca
    12241 aaccacatcg taccttttcc gggattcaga gcacggggca aacctttttg ccttaaagga
    12301 gtttgggcat atttacacac ggttaagcaa tcctacaacc gatgtacttg agcaacggct
    12361 tgctgcgcta gagggaggca aatcggctgt ggtggtatcg tcggggcatg ctgcgcagtt
    12421 tatagcgctt accaacatta tggagtcagg cgataatttc gtaagctctc cctacctgta
    12481 tggcggaagc cataaccagt ttaaggtcac ccttaaacgc ctgggcattg aggcaaggct
    12541 tgcaaaaagc cttaaaccgg aagattttga atgcttgatt gatagtaaaa ccaaagccat
    12601 ttacgttgaa accattggaa acccaagctt taacatcccc gattttaagg cacttacaac
    12661 cctggcaaac caacaccaaa tcccactaat tgttgataac acctttgcag gtgctggtta
    12721 cctttgcaga cccattgaat ggggggctaa cattgttgtg gaatcaataa ccaaatgggc
    12781 aggaggacac ggaaccagca tgggtggtgt catagttgat gccggcaact ttaactgggg
    12841 aaacggaaaa tttccacagt tttcggagcc atcggagggt taccatgggt tagtattctg
    12901 gactgcattt ggcgaggata gccctcatgg gaacattgca tttgccatta gggcaagggt
    12961 tgaagggtta cgcgattttg gcccagccat cagccctttc aatgcttttc aaatattaca
    13021 aggaattgaa actttatcgc tgcgcatgga aaggcattgc agcaatgccc ttgcgcttgc
    13081 caagtggctc aaaacaaaca ggcatgttga aagggtttgc taccccggac tacccgatga
    13141 cccaaaccac attcaagcaa aaaaatacct taaaaatggc tttgggggag tattgacatt
    13201 tacactcaag ggtaacctgg agcaaaccca aacgtttgtc gatagcctta agcttgttag
    13261 ccatcttgct aatgttggcg atgccaaaac gttgattatc caacccgctg ctacaactca
    13321 ccaacagctt agtgaagagg aacgccttgc agcgggcgtg ttgccaaacc tacttagggt
    13381 ttcggttggc attgagcaca tcaacgatat tattgccgac tttgaacagg cctttcaaaa
    13441 aacctttagc taactcaaag caatttaata cattgtggca gaaatctttt aaaaaaaacc
    13501 gacatgaaag taacggaagg caacctggaa cttaaaacac tccaacttga atgtggcgaa
    13561 gtgctatacg atgtaaacat tcgttaccat atactaggcg atattcaaaa aaatcccgag
    13621 agagttattt ggatttgcca tgcctttacg gccaacagca atccggccga ctggtggccc
    13681 gagatggttg gacaaggtag gttgttcgac cctgaaaaat acactgtggt gtgcgccaac
    13741 atcattggct catgctatgg ttcaaccggg cctatgagcg ttaaccctaa aacaggcaaa
    13801 ccttacctca ggagctttcc gttagtaacc ttccgcgatg tggttactgc gcaccaaatc
    13861 cttaaagagt accttggtat tgacaagata tgggtattaa ccggcggttc aattggcgga
    13921 ttccaggcca tggaatggga atgtatgtat cccggcacaa ccgagaatct tatactgatt
    13981 gccactgctg cccaggcttc gccttgggtt aaagcattca gccaaacgca acggcttgcc
    14041 atacttgccg atggaacctt tgacggtgct aatccaaacg gaggtaaagc agggcttaaa
    14101 gcagcgcgta gcattgcgct gcttagctac cgcggacgca gcagctacaa cctaacccaa
    14161 caagatagtg acccaaatca gctatcggac tttaaagcct gcacatacca ggactaccag
    14221 ggcgataaac tggttaagcg gtttgacgcc tactcctatt tcgccctgag cctaatgctc
    14281 gatagccata acatgggcag aggtagggta agcaatgaag cggcgttaaa ctcaataaaa
    14341 gcacgaacac tggttgtggg tatcgatagc gatatacttt tcccagttga ggagcaacat
    14401 ttcattgcag accacatccc aagcgcggtt taccgcgaaa ttcactccca gttctgccac
    14461 gatggatttc tgattgagta ccaacagcta acatcaatta tcagcgaatt catcaacctt
    14521 taaaaaatca acagaaatgg aaactttaac aatcacaaat acacgtaaaa actcaaagtc
    14581 aaacggagta aaccaacagg ttattggtct tttcggactt ggcaatgtgg gcaatgcgct
    14641 ttaccacgta attgctgaaa ttccaaagag caaagcatac ataaagcgca tctgtgttaa
    14701 gcaacgccat aaaaacaggg aggttccaca cgaattaatt acctacaatg ctgcggatat
    14761 aattaacgat gaggaaatta acctgattgt tgaacttatc gataacgccg atgaagcata
    14821 ccacatagta aaatcggcgt tacttagagg caagagtgtg gtttcaggga acaaaaccat
    14881 gctggcacac catcttccgg aactaattgc catacagcat gaaacgggag cggctttgct
    14941 ttacgatgct tcagcatgtg ggagtatacc tgtaattcgg aaccttgaag aatactacga
    15001 caacgacctt ttaaaatcga ttcgtggcat ccttaatggc tcaacaaact atatcctttc
    15061 caacgtattc agccaagggg tagattatca ttgggcgcta aaacaagcac aggaactggg
    15121 ttttgccgaa agcaaccccg attttgatgt gctaggattc gatgcactct ataagctgat
    15181 aatacttgcc gtgcatggat ttggtacata cgttcaccct agcgatgcct tttgctatgg
    15241 cataaacaat cttaaggctt acgacataca gtttgcccgc gaaaagggga aaaagttcaa
    15301 gctggttgct cagctacaaa agctaaatgc cgactacttt acactttttg ttatcccaac
    15361 actggtagaa ccctcggatt atatctataa tgttgagaat gagtttaacg gggtggttgt
    15421 tgagggacaa tactacgacc ggcaattcat gtttggtaag ggtgcgggcg gatacccaac
    15481 cggttcggct gtgctgagcg acattacagc tcgcttccat aactaccgtt acgagtataa
    15541 aaagcaaaaa tactttacgc caccagccta ttcaacagac gttgagctgg agctctatat
    15601 ccgataccac aacctaactg atgtcagtat ttttggggaa cgcaaggtta cagaacaaca
    15661 tttcgccacc gattaccgat gggaaatagc atgggtaaaa ctcaatgaac ttatcagagt
    15721 aaaggagttg cttccaaagt taaacctgtt tcttgctttt accggtaact ttaggtttaa
    15781 ccctattgac aaggcactat caagcaaggt aacttgtgct gatgattacc aaccaattgt
    15841 tgatgcattc tgaacattga ttttatggaa aaaacaacag tttttaggga gttactgaaa
    15901 agcagggtac tggtgctgga tggtgccatg ggcaccatga ttcaaaggta cagactaaag
    15961 gaggaggatt ttagaggcga tattctcaag aatcacccgg taaaccttaa gggcgacaac
    16021 gacctgctta accttacccg ccccgatgtg atagggcaga tacaccgtgc ctaccttgag
    16081 gctggagccg acataattga aaccaacacc tttaactcca cacgcatttc gcaatcggac
    16141 tacggtttgg agcagatggt ttacgagcta aacctagccg gagccaggct tgcccgttcc
    16201 attgccgatg agttcagtaa tgccaatccg caaaaaccgc gatttgtagc cggttccata
    16261 gggccaacca accgtactgc cagcatgtcg cccgatgtgg agcgtcctgg ctaccgtgca
    16321 gtaacctttg accagcttgt aaactcatac acagagcaag ctgaaacgct tattgatggt
    16381 ggggttgata tcctgctaat tgaaaccata ttcgataccc ttaacgccaa agctgcgctg
    16441 tttgccattt cagagatact tgaacgcaga ggtatcgata ttcctgttat ggtgtcggca
    16501 accatagccg ataacggggg acgattgctt accgggcaaa cccttgaggc atttgtctgc
    16561 tctgtatcgc acttcccgct actaagcatt ggcttgaact gcgcctttgg cgcagaacag
    16621 atgtatccgt ttgtcgagga actttcggct attacaccat tttttgtaag cgcacacccc
    16681 aatgccgggc taccaaatca gtttggggaa tacgaccaat cggcgcagga aatggctgaa
    16741 attgttgagc aaatgctagc caatgggctg gtaaacattg taggaggatg ttgcggaacc
    16801 acacccaagc acattgagct tgttgccaac ctggcccaac gctataaacc caggattata
    16861 ccagaaattc cacgccatac tcgactaagc ggtttggaat tgcttgagat aagacccgaa
    16921 agcaatttca tcaacattgg cgagcgaacc aatgtggcag ggtcaaaaaa gtttgcccgc
    16981 ttgatagccg aaggtaagta tgccgaggcc ctttcggttg cccgtgaaca ggttgagggt
    17041 ggcgcccagg ctattgacat ttgcatggac gacgccctaa ttgatgctcc aagagcgatg
    17101 actgaattcc taaacctgat agcatcggaa cccgacgttg cccgtattcc tgtaatgatt
    17161 gattcatcgc gtttcgatgt aattgaggca gggctgaaat gcactcaggg gaaaagctta
    17221 gtaaactcaa taagtttgaa agagggtgaa acggagtttg cccgtagggc aaaagttatt
    17281 aaacggtatg gcgccgcagt ggttgtgatg cttttcgacg aaagcgggca ggctgttact
    17341 accgagcacc gctgccgcgt tgccgaacgt agctaccgaa ttttaaccga aaaggttggc
    17401 ttttcgcctg aggatatcat tatcgaccca aatatccttg ccattggaac aggcatggtt
    17461 gaacattcaa cacaagccgt tagctttatt gaaaccatta aatggataaa acagaaccta
    17521 ccctacgcca aagttagcgg tggcgttagt aacctttcat tctcgttcag gggcaacaac
    17581 ctggtaaggg aggccattca ctcggtattc ctttaccacg ccattcaggc aggtatggat
    17641 atgggtattg taaacccatc gctgctgatg gtttacaccg atattgaacc aaatttactt
    17701 aaactcactg aggacttagt gctaaatcgc cggcgcgacg caaccgaaag gcttttggcc
    17761 tatgccagta agcttgaaga aactgacctg cagcatcaca aaaccgacga ctggcgttta
    17821 ctacccgttg agcaaaggct taagcactca cttatcaagg gaaccgatga gtttattcgg
    17881 gttgatgtgg aggaagccta caacaagcta aaatcgccta tcagtgtaat tgaggggcca
    17941 ttgatggatg gaatgcgcga ggtgggcaat ctttttggta caggccgaat gtttctgccc
    18001 caggttgtta aaagtgcgcg cgtaatgaaa caggctgtaa aggttttgga accctacctc
    18061 gaagcacaag caatggattc acataggaca gaacaaaaga aaatagtact ggcaactgta
    18121 aaaggcgatg ttcacgatat tggtaagaat attgttggtg tggttctcgc ctgcaacggt
    18181 tacagcatca tcgatttggg ggtaatggta ccagccgaga gaattgtgga tgaagtaaaa
    18241 gcaagtaaag ccgatattgt tggccttagc ggccttatca ctccctcgct cgacgagatg
    18301 gtacatgtgg taaaggaact aaaacgaaac ggcattaaca ttccggttct cattggtggt
    18361 gctaccacat cggaactgca tactgccctg aaaattgcac ctgaatactc gggagcagtt
    18421 gtccatgtta gggatgcctc acaggcaagc ggtattgttg ccagcttgct gagcactgac
    18481 aagtccgcaa gtaccataaa tgcctaccaa aagcgttacc agcaacaacg cgaaagctat
    18541 tcagggagta aaactgtttt agttccaatt gagcaagccc gcaaaaatag ccttaagctg
    18601 aactgggata gctacaaacc agttaggcca aagcaaatag gcatcaatac cttaagcaat
    18661 atcgatttaa atgagctaat acactatatc gattggacat tctttttcag ggcatggggt
    18721 ttaaaaggcc gttaccccga tatacttacc gatcctgttc atggcagcga agcacaaaaa
    18781 cttaaagcgg aagccctggc aatgctcaag caaatcagtg aacagaagct tttaaccccc
    18841 gaaggggttt acggaatatt cccagccgct tccagtggcg acgatgttat tgtgtacacc
    18901 gacgaaaccc gaacatcgcc cctaacccat ttgccgttcc tgcgaaacca ggagcaaaag
    18961 ccagagggtg agccaaatat gtgcctttct gactttattg cgcccgcaaa ttcaggcatt
    19021 aacgattgga taggatgttt tgcggttact actggctcca ccacatcaac acttattgag
    19081 gagtacagga aaaatggaaa cgactttgcc gccataatga ttcaaatttt ggccgacagg
    19141 cttgccgaag cttttgctga atggctacac ctaaaaatcc gaaaggactt ctggggctac
    19201 tcacccaacg agtcgctaac cgtggaacag ctgctgcagg aaaaatatgt aggcataagg
    19261 cctgctccgg gttacccggc ttgccccgat catcgtggta agcaatggat ttttcagctt
    19321 ctcgatattc cacggaatgc ttccataagc cttactgaga atatggccat gaacccattg
    19381 gcctccgtgg caggcttcta ctttgccagt agtgcaagca agtatttcag cgttggtaaa
    19441 attgacaacg accagctggc ggactatgcc aaacgaattg gtgttagtac aaccgaagca
    19501 gctcggttgt ttccaacctt tttagcaaac caataaaatt tactgagatg aaggtaatcg
    19561 atatacttaa taattcaaag tcgacacttt tttcctttga acttttacca ccgctcaagg
    19621 ggcataacat tgaaaccatt tttgccacaa tcgacaaact tttgccctac aacccgacat
    19681 atatcaatat aacctaccac cgcgaggagg tggtttacat gtccaacgaa aacgggatac
    19741 aggttcctgt aaaggttagg cggcgccccg gcaccattgg acttgccgct gcaatacagc
    19801 agaggtacgc aattgacgtt gtgccccata ttatctgcgg cggatttgac gcccacgaga
    19861 cagaggatgc ccttattgac ctcgattttt tgggaataca aaacatactc gcagtgcgtg
    19921 gcgatgccga taagattacg ggcaggtttg aacccagggc aggaggacat aggtatgcca
    19981 ttgacctggt gaaacaggtt actgacctga accgtggcgt ttaccttgaa aagctgatgg
    20041 aaaaacccaa accaactaac ttttgcatag gggtggctgg gtaccctgaa aaacaccctg
    20101 aatcgccaaa ctgcgagtcg gatattcact acctaaagaa aaaggttgaa gccggagcaa
    20161 gctacattgt tacccaaatg tttttcgaca acaagcttta cttcaacttt gttgataaat
    20221 gccgtaaggc cggaatagct gtccccatcg ttcccgggtt gaagcccatc agcataaagg
    20281 agcatcttag cattcttccc agggtgttcg atgttcatat acctcaagcg ctggttcttg
    20341 aactggaaaa atgtaagaca aacaaccagg tagcgcagct tggagtggaa tgggcaattg
    20401 aacaatcaag ggaactaaag agttcgggag taaaagccat ccatttctac accatgggta
    20461 aggacgataa tattgcacaa attgcaaaag ccgtatttta gtcattgtta gcattgctta
    20521 cacttattat cctgagcaga gcgcaggaaa atattcatta cttgcttaat ggtattttca
    20581 gccctctaca cccaaatcgt ctagctagga atgcttgtca tgttgagcta atcgaaacat
    20641 ctcaaataaa gcggtacaga gagatccttc gctccccgcc aaggcgttgt aggttgctca
    20701 ggatgacaaa ggagtgattt ttaccaacta aactaacagt gaatcagcac cccttaaaaa
    20761 gtttttggat taccgccgta aaagtcgtat cttacgaaaa aatctactat acctatggct
    20821 caaaaaaaag tgggcgaaaa gaggtttgaa ggtagctttg aatacgatgg cgaacttaag
    20881 tataggctga tatttgaaaa atcgcctatt ggtttatttc agtataacac cgatgctgtt
    20941 atcaccgaat gcaacgaccg ttttgttgag atactaaaat caaagcgcga gctgctggtt
    21001 ggtttgaaca tgcataaaat taacgacaag tcagtattac cggccatact cgatgcactt
    21061 gatggaaaaa acggatacta cgaggggcca tacacaacca caaccagcaa cgctcagatt
    21121 tacgttttac tgaaagcagt gccattaacc aaccctaaaa ccggacaggt tgtgggagca
    21181 atgggaattg tagaggatat ttccgatctt taccgaacac aggaggagct aaccatcaag
    21241 gaggaacgtt tcagaatcct atcatcgctc acttccgatt cggcttcaat actcaccatt
    21301 caacccgacg gctcatttaa acgtgagtgg cttagctccg cactaatggt tcagctaggc
    21361 tataagcccg aggatgttga ttcatttgaa aaatgggcaa ccattgtcca ccccgacgac
    21421 attgatgttt tcaagagagc cattgggact atcacccaga aaggcgagaa agttacttta
    21481 gagtttagaa taaaggccaa aaatggcaaa gtacactgga ttgagaatac tgtttaccct
    21541 gaattcgata gcagcggaaa acctttccga ctgattagcg cagtaaagga cattaccgct
    21601 aaaaaagaac aggagcagga acttcagcaa cagcgcaacc tattaaaatc aatagtagac
    21661 aatgccccgc ttggcatttg ggttgttaac cccgacggct catacccaat aataaatcca
    21721 tggtttagcc aaaacatagg ctatggcaca tcgcaattct ccatgaccga ggaggaggtt
    21781 gcccaatgcc gccgtagcga tatgcaggca ctaaactccg atgggccaat tgaaaccacc
    21841 gaggaggtta cttttaccga tggcaaaaag cacattcttc aaatattcaa gcaaaagcta
    21901 acctcaacaa caggtgaaac cattggtgtt ttgggtattg caaacgacat tagcgaacgt
    21961 acccaatacg agaaggcact tgttgaggca ttggaaaagg ctgaggagag cgacaggcta
    22021 aaaacagcat ttctggctaa catgagccac gaaattcgca caccgctaaa cggtgttatt
    22081 ggctttgcca agtttctccg caacttccct gataccacgc tccaagagcg cgagaaattc
    22141 ctgggaataa ttagcaccag cgccgatcat ctccttaccc taatcaacga tattattgat
    22201 atctcaaaga ttgacgttgg tcagctaacc attaaccccg aagcggttaa cattaacgag
    22261 ctgcttaacg agatatacac tttcttctac aacgcaaacc ccgacttacc caagcgtgaa
    22321 atagctttta actacaccac ctcactgccc gactccaagg ccatggttaa tgccgaccgc
    22381 atgcgattac gtcaggtgct tacaaacctt ataaacaacg cccttaagtt caccgataag
    22441 gggaaagtgg aatttggcta caacataaat agcgaaacca atgagtttga gttctttgta
    22501 agcgataccg gtataggtat tgccaaggac aagcaggaaa ttattttcca gcgtttccgt
    22561 caggcctata ccgatataac caagcagtac ggtggcacgg gcttaggact ggctatctgc
    22621 aaatcattgg ttgagcttat gggcggcaga atatgggttg aatcggaaac gggtaagggc
    22681 tccactttcc gtttcacatt acctattaat gctcctacaa cccaaaatag gggaaaggtt
    22741 tacgggtacg aactccagga actgaaaaat ctactcaaag gtaaatccat tcttattgcc
    22801 gaggacgacc ccaactgcat gtactaccta aaaacagttc tcaaggactt tgacattaac
    22861 atacttgagg ctgataatgg tctactagca actgagatcc agtatcagca tcccgagata
    22921 gccgccatac ttatggatat caaaatgccg gtgatgaatg gctacgatgc cattaaaagg
    22981 atacgctcca ccaacgccca ggtgccaata attgtgcaaa ctgcacacac cttcagcaat
    23041 gagagggcta taagcatagc cttaggctgc aaccagttta taaccaaacc cattgactca
    23101 aactcgcttt accaaagcct ctactcggta ttgaaggaaa gccgtaggta ataaattcgt
    23161 aaattacttt tcaatgtcgt ttagttaaca aacataaaca tgcttgtcat cctgagcggc
    23221 ctgtcccgcc ctagcgggga gcgaaggatc tctaatcggc cccatgagat gtttcgctta
    23281 gctcaacatg acaatagatc catgaacaac taataacgca tcccgccccg taggggcgta
    23341 atatttgtaa caccatgaat attccctcat gtaatacgat gcacgctata gtattccaaa
    23401 gagtaaggaa gaaacgttgc atcgcaaatt aaaagtattg caaagatcgg tttacatgtt
    23461 ctttcttgtc ttgatacaag aaagaaccaa agaaaatcaa ggctgaaaag cc
//
LOCUS       DAOSAL010000038        22387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_1660_length_22387_cov_50.030360, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000038 DAOSAL010000000
VERSION     DAOSAL010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 22387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22387
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1660_length_22387_cov_50.030360"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="PK049_06685"
     CDS             complement(<1..266)
                     /locus_tag="PK049_06685"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOK61281.1"
                     /translation="MAKFLGLGNALVDILVQLPNDAKLFELNLPKGSMQLVDAERMQS
                     ILAQTEGLPRTLAAGGSAANTIHGLANLGMSTAFIGKVGDDSFG"
     gene            complement(335..408)
                     /locus_tag="PK049_06690"
     tRNA            complement(335..408)
                     /locus_tag="PK049_06690"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(372..374),aa:Asn,seq:gtt)
     gene            complement(502..1452)
                     /locus_tag="PK049_06695"
     CDS             complement(502..1452)
                     /locus_tag="PK049_06695"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOK61282.1"
                     /translation="MSKRIVFAEPIGLNRTTAEAFTAEMLKHDYEVVYFDTVPASQSD
                     LQQRIQGAEVLVISNHPIAASTLQSCPTLKLISVAFTGVDHVPLDYCRKHSITVCNAA
                     GYSTTAVAELTLALAVSLLRNIVPFDALTRNGGTRSGFLGGELAGKTFGILGFGAIGQ
                     RVANLALAYGCKVITHTRTPKPTPGVSFVELEKLFSDSDILSFHLPATQQTVGLVNKS
                     LISLMKPTAVIINTARGSIVDCMALSTALQQGQIAGAAIDVYEHEPPIEADHPLLKAP
                     NTILLPHIAYATHEAIGKRASIVLDNIQKWILQNPQNVVV"
     gene            complement(1494..2003)
                     /locus_tag="PK049_06700"
     CDS             complement(1494..2003)
                     /locus_tag="PK049_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HOK61283.1"
                     /translation="MRLDSQFYQRDVLEVAPDLLGKTLVRKFESGDELRVTITEVEAY
                     RGTDDLGCHASKGMTERNRVMYARGGLVYVYLIYGMYWMFNVVTSVENNPQAVLVRGV
                     TGADGPGKLTRLLAIDKSFYGEDLVTSNRLWLEDNGVTPSFITTPRIGIDYAGPYWSK
                     VPWRFLLST"
     gene            complement(1990..3384)
                     /locus_tag="PK049_06705"
     CDS             complement(1990..3384)
                     /locus_tag="PK049_06705"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOK61284.1"
                     /translation="MAMYLPILLSGLLSSNHRLGFEIVRLWLPVLLIPPIIAASQELT
                     GKEFRFLLWLFIFSTLIVTILGTYNYLAGDTADIRKISPFISHIRLALMVNLALAIMV
                     YFIAHQPPTKTLAILLHLFVLIWFLFFLFLLASFTGILMLVLLIVLIAIKYLFGLGSV
                     ARFVTITGLSTLILLMLSYLLHKYDSMNTIRLIPENSPKTTTVNGNPYQHDVLSKETE
                     NGFLVNINICEDELRNEWLKRSNLSYDGTDKKGQPLRSTLIRYLTSAGLTKDSVGLSR
                     LDSADIALVELGYTNTIFKKRATGFESRLYEFFYEFNKFRQSGTLTGGSVLRRLIYAR
                     AAWHVIKGSPILGVGYGDLPTAMNQFYDIYKVDLPQSYRFMPHNQYLTIWASAGIIGL
                     AVFLLSLALPFLFSSNLKAMPVLYFWVMILISMLFEDTMLTHTGISFVAVFSALFLFG
                     HSFPVVKKEDYAAR"
     gene            3383..3595
                     /locus_tag="PK049_06710"
     CDS             3383..3595
                     /locus_tag="PK049_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61285.1"
                     /translation="MKTKTIGWDFSRCNFMAQLPLKRKLITKAIVPMLTPIIEDGKLQ
                     RITAKAARNTFRWNFSPNIKVKFLQI"
     gene            3930..5372
                     /locus_tag="PK049_06715"
     CDS             3930..5372
                     /locus_tag="PK049_06715"
                     /inference="COORDINATES: protein motif:HMM:NF012372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HOK61286.1"
                     /translation="MADIKDVNIDFISDCCVEKTTRNSLSKAILTHYLHNFENGISKH
                     YEKDAFNIIDQYISHVKKEHNISIDKSILFNKLIKEVSEVPFPPPKKHSFTFIDLFAG
                     LGGFRIAMQELNGKCVFSSEWDKDAQKTYKENFGEYPFGDITKQEVKNFIPDSFDLLC
                     AGFPCQPFSKGGFQNGFEDTRGTLFFDICEIIEKHKPKYLLLENVANLASHDNGNTYR
                     VITKNLDKLGYYFPDKPLIISPDSFGIPILRPRVYIPCIRKEFAKGKESLIKNFREEI
                     EKHFVNTTFTIDKIIDPEIEERISPYEEKVLKMWDDFYKGIDIKVIGFPIWMEYFKFD
                     GDLNQFPLWKAKIISKNINLYQKNKKFIDDWLKKYDNLDWCVKTHRKMEWQAGDKYES
                     VFDCLIQFRPSGVRIKKPDKFSTLVAMNHRQIIGKLKRKISVEEAKLLQSFPKEYKLV
                     GTPNTIFKQLGNSVNVTVVKVIFNILSKYY"
     gene            5390..6337
                     /locus_tag="PK049_06720"
     CDS             5390..6337
                     /locus_tag="PK049_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61287.1"
                     /translation="MIEKLKQNNKIIINSDIDGVISGLLLTNFCNCEVVGFSNSADRV
                     WLDTSKCNSIYDGVYIDMYVPNKSTITIDQHIVAINNEHLEKIKINNNKINPNIYNPR
                     FLSPTNSYIKKYPFGTCHFIISMLCKNGINLSSICLNNSSDELMFIDYLLRADDAMNT
                     SISRYKINAQEWWNWLLEYSNKSETIKSLTSYLYSIDIEHATKIKTKITERLINQFKC
                     ERPDGGFIQIMNENGRLKQNLIDYILFISDLSNLKCFDLNISFKEFRGVHIRTKLSKD
                     QQDELMNHNSVNGEKVFSYAFVRSIDKAENFSYTVMVNQ"
     gene            6334..7473
                     /locus_tag="PK049_06725"
     CDS             6334..7473
                     /locus_tag="PK049_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61288.1"
                     /translation="MKKIEQILEHNIVNDFDERLTPNEIRDILKADLEILNKSNPYTC
                     IVNQKEIKIYVKQITYLGHPHLAFKKRIQISRGWQIGLKEENAYLMGVYKYKETILYA
                     IFDKKNFVTRVTNNSSAHVSTFDLLNAQQKGIFTKKDIRGNVITCVRKDLIKIVFSKI
                     VSNESVLCQEILLFENFKLSLNSIYHGIECYTELISNNYRNKFQPEWFGFFIEFKFEK
                     FLEENSNYKSICWYQSKKSKNDIDLDLNFNNKFLGDLKTHSNESSAILGNNIKNINMA
                     LEKYGKLWYIVFNHNTFRDSENNFEVTIFWNEQQKKDNLMSYSKKMKNRIELTDFMIL
                     EINEYNKKYLSVFNQGINSNKLPREPKIKIDKKMINNFLIYSSKF"
     gene            7630..8748
                     /locus_tag="PK049_06730"
     CDS             7630..8748
                     /locus_tag="PK049_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61289.1"
                     /translation="MAYYINLFSPDTYQKFSDSDKSISGVSDKLKDVASTIKPGDKLI
                     CYVTKLSRWVGVLEVTSNYFINDSPIFTQTADPYVVRFNVKPIIWLPLENAIPVHEDI
                     IWNNISFTKGIEKKSTKWTYMVKRSLCKLNDGDGQFLESVLLNQIEKPTLYPLGEAEQ
                     KKLKTLTVKTQNSKQVTVSIPENEDVPTITVNQQQSTQRDSIKIQALLAEIGERMNFK
                     IWVPRSDRQRVLEVWHPKTQCLLEQLPLNYDDATLKTIENIDVLWIKGRSIVRAFEVE
                     HTTSIYSGILRMADLMALQPNLNIRAHIVAPAERKEKVLQEISRPVFAFLEKGPLSES
                     CTFISYESIQELSNEKSLEYMNDNVLEKFEEYAEEADI"
     gene            9095..10198
                     /gene="meaB"
                     /locus_tag="PK049_06735"
     CDS             9095..10198
                     /gene="meaB"
                     /locus_tag="PK049_06735"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOK61290.1"
                     /translation="MEQKKKEEKSALIVNPGIEQPPMVNEDAIRRFKAKKRKLLSIEE
                     YMDGIKNGNIPILSQAITLIESSLQQHQELARELVEQCLPLSGRSVRIGITGVPGVGK
                     STFIEALGSTITAQGHRLAVLAVDPSSERSKGSILGDKTRMETLSADPNAFIRPSPSA
                     GSLGGVARKTRETIILCEAAGFDTIFIETVGVGQSEVAVHSMVDFFLLLMLAGAGDEL
                     QGIKRGIMEMADMIAITKADGSNIQKAKLAQAEYQNALHLFPATESGWVPQAVTCSSV
                     NKTGILEIWSTINQYMAFTKANGYFSHRRSQQAKYWMYETINEELKNRFYHSTQVSTM
                     IKWIEHEVTHNKLSSFAAAQKLIDVYFEKNGNK"
     gene            10204..10788
                     /gene="thiF"
                     /locus_tag="PK049_06740"
     CDS             10204..10788
                     /gene="thiF"
                     /locus_tag="PK049_06740"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS adenylyltransferase
                     ThiF"
                     /protein_id="HOK61291.1"
                     /translation="MTFDEIKQRLKHYTVGIAGAGGLGSNVAMHLTRVGIGKLIVADF
                     DVVQESNLNRQFYFRHQLGAKKVEALKTNLLAINPELNIVIHSIKLDALSIREIFSDV
                     DIMVEAFDRAEMKEVIAEVFQTEYPNIPLVMGNGMAGWGKSNEMRVNRFGNLYICGDG
                     VSEIADELPPLSPRVGICAAMQANVVLELLLGSK"
     gene            10903..11526
                     /locus_tag="PK049_06745"
     CDS             10903..11526
                     /locus_tag="PK049_06745"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOK61292.1"
                     /translation="MFSGIVEEPAKVVALEREKENLHITLTCSFVNDLKIDQSVSHNG
                     VCLTVVKKDNSTYTVTAIKETLEKSNLGQLKVGDKVNVERSMKIDSLLDGHMVQGHVD
                     QTAVCTDVQEADGSWYYTFEYDPSKGNITVEKGSIAVNGVSLTVVNSRENSFQVAIIP
                     YTYEHTNFHEIKKGTVVNLEFDIVGKYITKILKQYLESGQLNRLTGK"
     gene            11532..12590
                     /locus_tag="PK049_06750"
     CDS             11532..12590
                     /locus_tag="PK049_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOK61293.1"
                     /translation="MTVKKIFESPRSLSLTIASIVSVLAAIFFLFIVNFTQGQQLLKL
                     VAFTLSVFTITFLSSLLLVNLFVSKRIKPLYKALYSIDKANADLVDELDQNNLLQRLE
                     YDLRVWAEAKTDEIDRLKQMERYRKEFLGNVSHELKTPIFNIQGYVLTLLDGGLEDPS
                     INRRYLERTEKSINRLIGIVEDLELITKLEAGELQLNLERFNIISLIEETFEALEQRA
                     KHKSITLELDKEYSKPIWVYADKKRISQVLYNLIINSIFYGKENGVTTVSVSDIGTRV
                     QVEVNDNGIGIDQKDLPRIFERFYRVDKSRSRDQGGTGLGLAIVKHIIEGHKQVIGVR
                     SSLNQGTTFSFTLDKPKK"
     gene            complement(12609..13484)
                     /locus_tag="PK049_06755"
     CDS             complement(12609..13484)
                     /locus_tag="PK049_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61294.1"
                     /translation="MRLKVTPLVVLLLVLFLVNAEAQNRPPQNVRRVIVTSRYEVKDG
                     KRTINSRAIKQEIKDSLDRVHTILFRDYETQGVYRYIWHTYEGEQIVRTDEFANEKLF
                     RYSLFTYTPDSLVATQKVYLVNPGDTVHYVTLSFKYQNRLLIQVDAHNSKGKRVYRTK
                     SVYDNYGTEILRQVKVSKGYIPVDSIVNLSCKPTYDTLGRKVAETITKTFADGRKEAK
                     SFKYEYNKKNLRSAVEEFDSAGKLLSRTTLEYNDKGMLKFISVFNSEGQLIDYQALRY
                     ELYPSLDRRKQIIEY"
     gene            13608..14426
                     /locus_tag="PK049_06760"
     CDS             13608..14426
                     /locus_tag="PK049_06760"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HOK61295.1"
                     /translation="MHEDLRQLVEQAWDNRELLNQKEYIQGIEAVIDLLDKGQIRVAQ
                     PIEKGWLVNEWVKKAVLMYFPIRKLEVMEAGILQFYDKIPLKRDFSAIGVRAVPHAVA
                     RYGSYLAKGVVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKGVHLSGGVGIGGVLE
                     PVQAAPVIVEDGCFIGSRCIIVEGVHIGREAVLGANVVITQSTKIIDVSGPEPVEYRG
                     FVPERSVVIPGSYTKKFPAGDYQVSCALIIGKRKESTDLKTSLNNALRDFGVSV"
     assembly_gap    14428..14527
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            14854..15699
                     /locus_tag="PK049_06765"
     CDS             14854..15699
                     /locus_tag="PK049_06765"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOK61296.1"
                     /translation="MADIDFLKKMAAQVRRDVIRMVHKPASGHPGGSLGCADFFTALY
                     FEVMNIDPKNFTMDGTGEDVFFLSNGHLSAAWYSVLARRGFFEVAELSTFRLINSRLQ
                     GHPTTAEHLPGIRVASGSLGQGLSVACGAALAKKLNGDKNLVYTLHGDGELQEGQIWE
                     AALFAAAKKIDNLIATVDYNGKQIDGPVDEVVTLGNLKAKWESFGWEVLESNGNDMEK
                     LISTFALAKSKTGQGKPIVILMRTEMGMGVDFMMGTHKWHGKAPNDEEFARAMAQLPE
                     TIGDF"
     gene            15733..16134
                     /locus_tag="PK049_06770"
     CDS             15733..16134
                     /locus_tag="PK049_06770"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF003559.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HOK61297.1"
                     /translation="MQNIALIAHDAKKPELVRFLKEHEDWLPGVNLLATGRTAEFLEE
                     QGIKCRHLSPGKSGGYNEITEMIGKGEIDIVIFLRDHKVVQPHHEDIRRLLEACNVNN
                     IPLATNKATAELVILGLIRKESIERMKRNID"
     gene            16235..17185
                     /locus_tag="PK049_06775"
     CDS             16235..17185
                     /locus_tag="PK049_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HOK61298.1"
                     /translation="MKYTSTGNKDTRSGFGAGLHELGRTNPNVVALCADLIGSLKMDD
                     FIKENPNRFFQVGIAEANMIGLAAGLTVGGKIPFAGTFAAFATGRVYDQIRQVVAYSN
                     KNVKICASHAGLTLGEDGATHQMLEDIGMMKMLPNMTVIVPCDYNQTKAATLAIADHV
                     GPVYLRFGRPAVPNFTPADQKFEIGKAQLLVEGSHVTIFATGHMVWKALEAADILEQK
                     GISAEVINIHTIKPLDEEAVLKSVSKTRCVVTAEEHMMNGGLGDSIAQLLARRMPTPV
                     EMVAVNDTFGESGKPEELLVKYGLDTPNIVEAAQKVLARM"
     gene            17201..17749
                     /locus_tag="PK049_06780"
     CDS             17201..17749
                     /locus_tag="PK049_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOK61299.1"
                     /translation="MDNHTDSELLVMFRNPETKHYAFNLLVRKYQERLYWQVRKIVIS
                     HDDADDVIQNTFLKVWGGLENFREDSQLFTWLYRIATNEALTFLKKKRTKLLLPIVDV
                     EQQLARTLEGDPFIDGDSTALKLQKALLKLPEKQRLVFNMKYFEEMKYEEISEILGTS
                     VGALKASFHHAVKKIEKFLEED"
     gene            17820..18257
                     /locus_tag="PK049_06785"
     CDS             17820..18257
                     /locus_tag="PK049_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61300.1"
                     /translation="MKSMEEKFNKKLPFKVPEGYFDNLPLRVQERIAATETQHEPKSR
                     WVTLKSQLAFAAGFAFMALLSYLGYYMARPLMADKKAVKTDYVEIVSRTIYEFDDIDL
                     YKAIEQKKKTDSLKEVYNEIPHRLYVRSSNCITIIDEKKEIKP"
     gene            18254..18697
                     /locus_tag="PK049_06790"
     CDS             18254..18697
                     /locus_tag="PK049_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61301.1"
                     /translation="MRQLFRILIVFIFIALGAKAYSQTDDTEKALKAEKVAFLTKKLD
                     LTQDEAKKFWPLYDEYWEKKIKILNDRRKLADDFIQNINNITDKEAVDYARRYVESQK
                     RESDLIAEYNKLLLQILPPKKVMLLYQSNYEFKNYLLQKVQETKK"
     gene            18784..19173
                     /locus_tag="PK049_06795"
     CDS             18784..19173
                     /locus_tag="PK049_06795"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaC family regulatory phosphoprotein"
                     /protein_id="HOK61302.1"
                     /translation="MTELVDLFIPATTETPEIKCEKSSGRIMMVGNLIPSDPQSFLAP
                     IHRWLQSYVTTYTPQNITVEFYLTFINGCSEHHLGKLLKYLEELHINGMSVKVICHYE
                     NEDLDMKEWGKDLSMALRIPIELVEIN"
     gene            19274..19696
                     /locus_tag="PK049_06800"
     CDS             19274..19696
                     /locus_tag="PK049_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HOK61303.1"
                     /translation="MSINEIQDAIVEEFSVFDDWMDRYQQLIEYGKDLPPIDERKRTE
                     QYLIQGCQSKVWLDAELRDGKIYFTADSDAIITKGIVALLIKVLSGRTPDEILNAELY
                     FIDKIGLKENLSPTRSNGLVAMVKQMRMYALAFKAKAE"
     gene            19746..20060
                     /locus_tag="PK049_06805"
     CDS             19746..20060
                     /locus_tag="PK049_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008146258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HOK61304.1"
                     /translation="MRDELEIQTDIVKTLKGIHDPEIPVNIYDLGLIYEVDVDDNRKV
                     TITMTLTAPNCPLADQVVEEVQEKISKIDGVTKVEVRLTFDPPWDKSRMSDEAMLELG
                     FL"
     gene            20075..20986
                     /locus_tag="PK049_06810"
     CDS             20075..20986
                     /locus_tag="PK049_06810"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOK61305.1"
                     /translation="MEKKSQSRAYFLASFVVLFWGTSATAFKIGLSHTEPVQLLFWSS
                     LSASIILFVVLTFQKKLPLLKFTSKKEILYSLAESVLNPFLYYLVLFKAYSLLPAQVA
                     QPLNYIWPIVLVVLAALLLKHPLRFTDIIALLFSLIGVAFISSQGNINIFAKSNPLGV
                     LLAVGSSVIWASFWIVNMYDKHRPEEVKLFISFALATVLTLLYMAAIGDLPVFNLKPL
                     AASVYIGAFEMGITFVLWLKALNSATNTARLGNFSYLVPFVALVFVSLVLREKIVWTT
                     LVGLIVIIGAIVFQQLFSKKDIKDEPI"
     gene            20973..22226
                     /locus_tag="PK049_06815"
     CDS             20973..22226
                     /locus_tag="PK049_06815"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOK61306.1"
                     /translation="MNRFKLAILLLFGLAAFTSCEDDEQKVNVNKEVYNLMKDWYYWY
                     DQLPNVDPNSYPDPVELVKAIRVNPPDRWSYVTTKQELDAYYKQAAYIGFGFGSAFTA
                     DNKLIITFVFNTSPLYANGIGRGWEIVSIDGQTPTPDNYTSPIGPSEVGVTKTFVFKS
                     PSGQTTQHTFSKAQIEMNTVLLDSVYTINGKKVGYFVLKGFIEKTNDELNAVFQKFKS
                     AGVSELVCDLRYNGGGQVNVSQFLGNLIGGSIAFGRVFGQYIHNDKHSDKNSHLYFST
                     TQNSLELQRAIFITTSITASASELVINALKPFMDVVLVGSKTHGKPVGMYTFEFKDPS
                     IDWAIVPVCFSIRNANNDGDYYDGIPVTIEAADDVLTPLGEVTESSLNASLNYLGLGT
                     KVVTKRYVTAQPITGKGLYEEIGAW"
BASE COUNT         7010 a         4410 c         4684 g         6183 t
ORIGIN      
        1 tgaccaaacg agtcgtcgcc caccttgcca atgaaagcag tactcatgcc aagatttgcc
       61 aagccgtgaa tggtgttagc agcgcttccg cctgcggcaa gagtacgggg gagcccctcg
      121 gtctgggcca gtatgctttg catgcgctca gcatcaacta gctgcatgct tcctttgggt
      181 aaattcagct caaagagctt tgcatcgttt ggtagctgca caaggatatc aaccaatgca
      241 ttcccaagtc caagaaactt tgccataatc tatcagcggt ttaatcagat ggtaaatata
      301 caaaaaataa aaccccgatt ttcatcgggg tttgctcctc atctaggact tgaacctagg
      361 accccctgat taacagtcag gtgctctaac caactgagct aatgaggagt gctgttctgt
      421 tattgcggtg caaaaataat attttttgag aatacgaacc aaatttttta aaaaaatttt
      481 tatccaaatc gcccaatacc actaaactac aacattctga gggttttgca aaatccactt
      541 ttggatattg tccagaacaa tacttgcccg cttgccgatt gcctcgtggg tggcgtaagc
      601 aatgtggggt agtaaaatag tattgggggc tttaagtaat gggtgatcgg cctcaatggg
      661 tggctcatgc tcatatacat caatagcagc cccggctatc tgcccttgct gaagggctgt
      721 gcttaaagcc atacaatcga caatactgcc cctggcggtg ttaataatca ccgccgttgg
      781 cttcatcagg cttatcagcg acttattaac taaacccacc gtttgttgcg ttgcaggtag
      841 atggaacgat aaaatatcgg agtcactgaa caacttttca agctccacaa aggaaactcc
      901 gggagtgggc ttgggtgtac gggtatgagt tataacctta catccgtaag ctaatgccag
      961 gttagccact cgctgaccaa ttgcaccaaa cccaagtatt ccgaatgttt tgccagccag
     1021 ttcgccacct aagaaaccac tacgggtgcc cccgttacgg gttaaagcat caaaaggaac
     1081 aatattgcgg agtaatgaaa ccgctagggc taacgttaac tcagccactg ctgttgtact
     1141 atatcctgct gcattacaca cggtaattga gtgtttccta cagtaatcga gcgggacatg
     1201 gtcgaccccg gtaaatgcta ccgaaattag cttaagcgtg gggcacgact gtagggttga
     1261 tgctgcaatg ggatggtttg agataacgag cacttcagca ccctgaatgc gctgctgcaa
     1321 atcggactgt gaggctggaa cggtatcgaa atacaccact tcatagtcat gtttaagcat
     1381 ttctgctgta aacgcttctg ccgttgtcct gttaagccct atgggctcag caaatactat
     1441 tcttttactc attgctaaac ttttttatca atactgctgg taatgaatac aaactatgtg
     1501 ctcagaagaa accgccatgg tacctttgac cagtatggcc cggcatagtc aattccaatg
     1561 cggggcgtgg ttatgaaact aggtgtaact ccgttatcct ctagccatag gcggtttgag
     1621 gtcaccaaat cctcaccata aaaggacttg tcaatggcaa gcaagcgggt aagctttcct
     1681 gggccatcgg ctcctgttac cccccttaca agtacggcct gtggattgtt ttcaaccgag
     1741 gtaaccacat tgaacatcca gtacatcccg tagataaggt aaacgtaaac cagcccaccc
     1801 cgtgcgtaca tcaccctgtt acgctcggtc atgcctttgc tggcatggca acccaaatca
     1861 tcggttccgc gataggcctc aacctcggtg atggttaccc gtaactcgtc cccgctttcg
     1921 aattttcgaa ccagtgtttt gcccagcagg tctggggcca cctcaagaac atcgcgctgg
     1981 tagaactgac tatcgagccg cataatcttc tttctttact actgggaaac tatgcccaaa
     2041 aaggaaaagc gcactgaaca ccgctacaaa cgatatgcct gtatgggtaa gcatggtatc
     2101 ctcaaaaagc atcgaaatca gaatcattac ccaaaaatag agtactggca ttgcctttag
     2161 attcgacgaa aataaaaagg gtagagctag cgagagcagg aaaactgcta acccgataat
     2221 tcctgccgaa gcccaaattg ttaagtactg attgtgtggc ataaaccggt aactctgtgg
     2281 taaatcaacc ttataaatat catagaattg gttcatggca gtaggcaaat cgccgtaacc
     2341 aacaccaagt attgggctcc ccttaataac gtgccatgcc gcacgggcat aaatcaaacg
     2401 gcgaagaaca gaacctcctg ttagtgtgcc tgattgcctg aacttattaa actcatagaa
     2461 aaactcgtag agtcggctct caaaacctgt tgccctcttt ttgaaaattg tatttgtata
     2521 ccccagctca actaaagcga tatcggccga atcgaggcga cttaatccga cagagtcctt
     2581 tgtaagccct gccgatgtta aatagcgtat taaggtgctc cttaagggtt gacccttttt
     2641 atcggttcca tcgtacgata gattgctccg tttaagccac tcgttccgta gttcatcttc
     2701 gcaaatgttg atatttacaa gaaacccatt ctccgtttcc ttggataaca catcgtgttg
     2761 gtagggattt ccatttactg tagtagtttt tgggctattt tccgggatta gcctaatagt
     2821 attcatgcta tcatacttgt gcaataagta cgataacata agtaaaatca atgttgataa
     2881 accagtaata gtaacaaagc gagcaacaga gccaagccca aacagatact tgatagcaat
     2941 tagcactatc aaaagaacta gcattaaaat gccggtaaac gaagccaaga gaaagaggaa
     3001 aaacagaaac catatcaaaa caaagagatg aagcagtatg gcaagggttt tcgttggcgg
     3061 ttgatgcgca ataaagtaaa ccatgatggc aagggcaaga tttaccatca gtgcaagcct
     3121 aatatgcgat atgaacggcg atatttttct aatatcggct gtatcgccag caaggtaatt
     3181 gtaggtacct agtattgtaa ctattaaagt ggaaaaaata aaaagccaga gtaaaaacct
     3241 aaactcctta ccggttaatt cctgggaagc agcaataatt ggtggtatca gtagaacagg
     3301 cagccaaagg cggacaatct caaagcctaa acgatgatta ctggaaagca atccgctaag
     3361 caggataggt aaatacattg ccatgaagac caaaaccatt ggttgggact tcagccgctg
     3421 taactttatg gcccagctgc cgctcaaaag aaagttaata accaaggcaa ttgttccaat
     3481 gcttacccca ataattgagg atggtaaact gcaaagaatt acggccaaag cagcaaggaa
     3541 tacattccga tggaacttct caccaaacat caaggtcaaa tttttacaaa tatagctatt
     3601 tgtagagtgg acgagcagcc tatatgttac cagtagttaa acgaatccag gagaggggct
     3661 ggggatgagg tcgcttttac catcaaccat caactttact tgtctgttaa atttgattat
     3721 aaaaaaataa gattcctttt gatgatgtaa aatatttttt ttagttattg caaatagtcc
     3781 atcaaataac aaacctttac acccaaataa gatgtataaa gataattttt ttaactattt
     3841 atgtgtaatg attaataaaa tgtagacgag attttccaac taaaatattt gagtttttta
     3901 gtacctttgc gagaaagatt taaacactta tggctgatat taaagatgtt aacattgatt
     3961 ttataagtga ttgttgcgtt gaaaaaacaa caagaaattc tttgagtaaa gcaattttaa
     4021 ctcattatct tcataatttt gagaatggga tatcgaagca ttatgaaaag gatgccttta
     4081 atattattga ccaatatatt tctcacgtta aaaaagaaca caatatcagt atagataaat
     4141 caatattatt caacaagctt ataaaagaag tttctgaagt tcctttccct ccaccaaaaa
     4201 agcattcatt tacttttatt gacctatttg cagggcttgg tgggttcaga attgcaatgc
     4261 aagaactgaa tggaaaatgt gtttttagta gtgaatggga taaagatgcg caaaaaacat
     4321 acaaagagaa ttttggagag tatccatttg gtgacataac aaaacaagaa gtaaagaact
     4381 ttattccaga tagttttgat ttactttgtg caggttttcc ttgccaacct ttctcaaaag
     4441 gtggttttca gaatggtttt gaagatacta gaggtactct attttttgat atttgtgaaa
     4501 taatagaaaa gcataaacct aagtatttac tattagaaaa cgttgcaaat ttagcaagcc
     4561 atgataatgg aaatacttac agagtaatta caaaaaattt agataaactt ggctactatt
     4621 tccccgacaa accattaata atatcgcctg atagttttgg aataccaatt ttacgcccac
     4681 gtgtttatat accgtgtata agaaaggaat ttgcaaaagg caaagagagt ttaataaaga
     4741 atttcaggga agagattgag aaacattttg ttaataccac tttcactatt gacaaaataa
     4801 tagaccctga aattgaagaa agaataagcc cctatgagga aaaggttctg aaaatgtggg
     4861 atgattttta caagggaatt gacataaaag tgattggttt ccctatttgg atggaatatt
     4921 ttaaattcga tggggatttg aatcagtttc cactttggaa agctaaaatc atttcaaaaa
     4981 acataaacct ttatcaaaag aataaaaaat tcattgacga ttggcttaaa aaatatgaca
     5041 accttgattg gtgtgtaaaa acacaccgaa aaatggagtg gcaggctgga gataaatatg
     5101 aaagtgtttt tgattgctta attcaattta gaccgtcagg cgtaagaatt aaaaagccag
     5161 ataagttctc aacgttagta gcaatgaacc atcgtcaaat cattggaaaa ctcaagagaa
     5221 aaatatcggt tgaagaagct aaacttttgc aatcattccc aaaggagtac aaacttgttg
     5281 gtacaccaaa tactattttt aaacaacttg gcaattcagt caatgtaact gtagtcaaag
     5341 taattttcaa tatactttct aaatactatt aattctttat cataaataaa tgattgagaa
     5401 gttaaaacaa aacaacaaaa taataatcaa ctctgatatt gatggagtta ttagcggttt
     5461 gttgttaacc aatttctgca actgtgaagt tgttggtttt tcaaatagtg cggatagagt
     5521 ttggcttgac acttcaaaat gtaactctat atatgatggt gtatacatag atatgtacgt
     5581 tcctaacaaa agtacaatta caattgacca gcacattgtt gctattaaca atgaacattt
     5641 ggaaaaaatt aaaatcaaca ataacaagat taaccccaat atatataatc caagattttt
     5701 atctccaact aactcataca taaaaaaata tccatttggc acatgtcatt ttatcatttc
     5761 aatgctatgt aagaatggta ttaacttgtc aagcatttgt cttaataatt catcagatga
     5821 attgatgttc atagactatc ttctaagagc tgatgatgct atgaacactt caatttctag
     5881 atataagatt aatgctcaag aatggtggaa ttggctttta gagtattcta acaaatctga
     5941 gacaattaaa tctcttacat cttacttata ttcgattgac atagaacatg ctactaaaat
     6001 taaaactaag attaccgaaa gattaattaa tcaatttaaa tgcgaaagac ctgatggtgg
     6061 tttcattcag ataatgaacg aaaatggtag gttaaagcaa aatttaattg attatattct
     6121 gtttatttcg gacttgagta acctgaaatg ctttgattta aatatttctt ttaaagagtt
     6181 tagaggtgtt cacataagaa ctaaactttc caaagatcag caagatgagt taatgaatca
     6241 taattcagta aatggggaaa aagtcttttc ttacgcattt gtaaggtcta ttgataaagc
     6301 ggaaaatttt agttatacag taatggtaaa tcaatgaaaa aaatagaaca aatattagaa
     6361 cacaatatag taaatgattt tgatgaaagg ttaacaccaa atgaaatcag agacatatta
     6421 aaggcggacc ttgaaatact aaacaaatca aatccatata cttgtatcgt taatcaaaaa
     6481 gaaataaaaa tatatgtaaa acaaataact tatttagggc atcctcactt agcgttcaaa
     6541 aaaagaattc agatttctag aggttggcaa atagggttaa aagaagaaaa tgcctattta
     6601 atgggtgttt ataagtataa agaaacaata ctttatgcaa tttttgataa aaagaatttt
     6661 gtaacacgtg ttaccaataa ttcttctgct cacgtctcaa ctttcgacct tttaaacgcc
     6721 caacaaaaag ggattttcac aaaaaaagac attcgaggaa acgtaataac ctgcgtaaga
     6781 aaagatttaa taaaaattgt tttttccaaa attgttagca atgaaagtgt tctatgccaa
     6841 gaaattttgt tatttgaaaa ttttaaactt tctctaaact caatctacca tggtattgaa
     6901 tgctatactg aactaatatc aaataattat cgaaataaat ttcaaccaga atggtttggt
     6961 ttctttattg aattcaaatt tgaaaaattt ttagaggaaa actctaacta caaatctatt
     7021 tgttggtatc aatctaaaaa atcaaaaaat gacattgact tagatttgaa ttttaacaat
     7081 aaatttcttg gcgacttaaa aacacattct aatgaatcaa gtgcaatttt aggtaataat
     7141 ataaagaata taaacatggc gctcgaaaaa tatggtaaat tatggtatat tgtttttaat
     7201 cacaatacat tccgtgattc tgaaaacaac tttgaagtaa ccattttttg gaatgaacaa
     7261 caaaagaaag ataacttaat gagttattca aagaaaatga agaatagaat tgaattaact
     7321 gattttatga tacttgaaat aaatgagtat aataaaaaat acttatcagt ttttaatcaa
     7381 gggattaact ctaataaatt accaagagaa ccgaaaataa aaatcgataa gaaaatgatt
     7441 aacaactttt taatatacag ttcaaagttc taaaacatag aacataacaa gtgttgcatt
     7501 gcatgttagg aatctgctag atatgatttt tttgcttcaa accatttttc ttaacattgt
     7561 acgacttggt tcaaaacacc agcccgcaaa cgtttcggtg caaccgaaaa agacaaaaaa
     7621 cattaacata tggcatatta cattaactta ttctcacccg acacgtatca aaagttttcg
     7681 gactctgata aaagtatttc aggagtttca gacaaactaa aagatgttgc ttcaactatc
     7741 aaaccaggcg ataaactaat ttgttatgtg acaaaattat cacgctgggt tggagtttta
     7801 gaggtaacga gtaactattt tataaatgac tcaccaattt tcacccaaac ggccgaccct
     7861 tatgttgtaa gatttaatgt aaaaccgata atctggctac cattggaaaa tgctatacca
     7921 gttcatgaag atataatttg gaataatata tcatttacta agggtataga aaaaaagagt
     7981 accaaatgga cttacatggt taaaagaagt ttgtgtaaac tgaatgacgg agacgggcaa
     8041 ttccttgaaa gtgttttatt aaatcaaata gaaaaaccta ctctatatcc cttaggtgaa
     8101 gctgagcaaa aaaagttaaa gacgctgaca gtaaagacgc aaaacagtaa acaggtaacc
     8161 gtttcaatac ccgaaaacga agatgttccc actataaccg taaatcaaca acaatcaacc
     8221 caacgtgatt ccattaaaat acaagcacta cttgcggaaa ttggcgaacg gatgaatttt
     8281 aaaatttggg taccacgtag tgacagacaa agagttttgg aagtttggca tcctaaaaca
     8341 caatgtttac ttgagcaact tccgttaaat tatgacgatg ccactttgaa aacgattgaa
     8401 aacattgatg ttttgtggat taaagggcgt tcaattgttc gtgcttttga agttgaacat
     8461 acaacatcaa tttattcagg aattttaaga atggctgacc taatggcctt acagccaaat
     8521 ttaaatatta gggctcacat tgtggctcct gccgaaagaa aagaaaaagt cttacaagaa
     8581 atatcaaggc cagtttttgc atttttagaa aagggtcctt tatctgaatc gtgtacattt
     8641 atatcttatg agtcaattca agaactttcc aatgaaaaga gtttagaata tatgaatgac
     8701 aatgttcttg aaaaatttga ggaatatgct gaagaagcag acatatagac aaaaacggaa
     8761 gcctttaata aaactgtaat ttttaatcct gttaaaatta cattcatgct tatttgagca
     8821 gaaccgatgc taaatcgcac aacttcatat ttcttagcca tttgggcaga ttgcttatca
     8881 tgacaatgtt tggaagttaa tttaatcgct acattattgc aatcatcttg taaccaacag
     8941 ccctcatcta cttcaaccgc aacgagctgg tagcttaatg aaccaaggat gttcgtaaac
     9001 aaaaagaaat tgtgcctctt tttaagtaaa cccattcacc caattaggtt ttacagaata
     9061 gaatgaataa attcgcgtat ccaagcgata cgaaatggaa cagaaaaaga aagaggagaa
     9121 aagcgcactt atagtaaatc cgggcattga acagccaccc atggtgaatg aggatgccat
     9181 taggcgattc aaggcaaaaa agcgcaagct cctgagcatt gaggagtaca tggatggtat
     9241 caagaatggc aatattccca tactcagcca ggcaattacc cttattgaga gttcgcttca
     9301 gcaacatcag gagctggcaa gggaactggt tgagcaatgc ctcccccttt cgggtcgttc
     9361 ggttaggatt ggtataaccg gtgttcccgg agttggtaaa agcactttca ttgaggcgct
     9421 gggaagcacc ataaccgctc agggacacag gctagccgtg ctggcagttg acccaagcag
     9481 cgagcggagc aagggaagca tacttggcga taaaacccgc atggaaaccc ttagcgccga
     9541 tcccaatgca ttcatcaggc cttcgccctc ggcgggttca ctgggcgggg ttgcccgcaa
     9601 aacgcgcgag accattattc tttgtgaagc tgccggtttc gatacaatat ttattgaaac
     9661 ggttggtgtt gggcaaagcg aggtggcggt tcattccatg gtcgatttct tcctgctgct
     9721 tatgcttgcc ggcgccggtg atgaacttca gggaattaag cgcggcataa tggaaatggc
     9781 cgatatgatt gccattacca aagccgatgg aagcaacatc caaaaggcaa agcttgccca
     9841 ggccgagtac cagaacgcct tacacctttt ccctgccacg gaatcgggat gggtacccca
     9901 agcggttaca tgctcatcgg ttaacaaaac aggtatattg gagatttgga gtaccataaa
     9961 ccagtacatg gcatttacca aggcaaatgg ctactttagc cacaggcgaa gccagcaggc
    10021 aaagtactgg atgtacgaaa ccataaacga ggagctgaaa aaccgttttt accacagcac
    10081 acaggtgagt accatgatta aatggattga gcacgaggtt actcataaca agcttagctc
    10141 atttgcggcc gcacaaaaac taatcgatgt gtattttgaa aagaatggta ataagtagag
    10201 tgtatgacct ttgatgagat aaaacaacgc ttaaagcact acacggttgg cattgccggt
    10261 gcaggtggac ttggcagtaa tgtagccatg cacctcacgc gggttggcat tggaaagctg
    10321 atagttgccg actttgatgt ggttcaggaa agcaacctga acaggcagtt ctacttcagg
    10381 caccagcttg gcgccaaaaa ggttgaggcg ctaaaaacaa accttttagc cataaacccg
    10441 gaacttaaca tagtaataca cagcattaag ctcgatgccc taagcatcag ggaaattttt
    10501 agtgatgttg atattatggt tgaggccttt gacagggccg aaatgaaaga ggtaattgcc
    10561 gaggttttcc aaaccgaata ccccaacatt cccctggtaa tgggcaatgg tatggccggt
    10621 tggggtaaat cgaacgaaat gcgggttaac cggtttggca acctttacat ctgcggcgat
    10681 ggagtatcgg agattgccga tgagctgcct cccctctcgc ccagggtggg tatctgtgca
    10741 gccatgcagg ccaacgtggt tttggaattg ctgttgggta gcaagtagta taagtcaagt
    10801 cctgcagctt cataaagaag cgatagcctt ttaatacaaa atctgtttga agcaaaatca
    10861 atttatacta tatctttgca tcaaaagtaa aaatcaaact ttatgttttc aggaatagtt
    10921 gaggaaccgg caaaagtggt tgcgttggag cgggaaaagg agaacctaca tatcactcta
    10981 acctgctcgt ttgtaaatga tttaaagatt gaccagagcg tttcgcacaa cggcgtttgt
    11041 ttaactgtag tgaaaaagga caactctact tacactgtta ccgccattaa ggaaaccctt
    11101 gagaaatcga acctagggca actcaaggta ggcgataagg ttaacgtgga acgcagcatg
    11161 aaaatcgata gcctgctcga tgggcatatg gtacaaggcc atgttgacca aactgccgtt
    11221 tgcaccgatg ttcaggaagc cgatggtagc tggtactaca cttttgagta cgacccctcc
    11281 aaggggaata ttaccgttga aaagggttca attgccgtaa acggcgtaag cttaacggtg
    11341 gttaactccc gtgagaactc gtttcaggtg gctataatcc catatacata cgagcatacc
    11401 aactttcatg aaattaagaa aggaaccgtt gtaaacctgg agttcgatat tgttggtaaa
    11461 tacatcacca aaatacttaa gcagtacctg gaatcgggcc agcttaacag gcttaccggc
    11521 aagtaaaaca catgacggta aaaaaaatct ttgaatcgcc acgttccctc tcgttaacta
    11581 ttgcatctat agttagcgta ctggctgcaa tattttttct gtttatcgtc aactttacgc
    11641 aggggcaaca gctgcttaag cttgttgctt ttacgctttc tgtttttaca ataacctttc
    11701 tgagtagcct tttactggtc aacctatttg tgtcaaaaag gattaagcct ctatacaagg
    11761 ctttatacag cattgataag gccaacgccg accttgttga cgagcttgac cagaacaacc
    11821 tgctacagcg gctcgagtac gacttaaggg tatgggccga agcaaaaact gacgaaatag
    11881 accggttaaa gcagatggag cgttaccgta aggagttcct gggaaacgta tcgcacgagc
    11941 ttaaaacccc catttttaat attcagggat atgtgctcac cctgctggat ggcggactgg
    12001 aggacccaag tattaatcgg cgttacctgg aacgtaccga gaaaagcata aaccgcttaa
    12061 ttggtattgt tgaggatctg gagctaatca caaagcttga ggctggtgaa ctgcagctca
    12121 acctggaacg cttcaacatt attagcctga ttgaggaaac atttgaggct ttagagcaac
    12181 gggcaaaaca taaatcaatc acccttgagc tcgataaaga atacagcaaa cccatctggg
    12241 tttatgccga taaaaaaaga atctcgcagg tactttataa tcttattatc aactccattt
    12301 tttatgggaa ggagaacggg gtaaccactg tatcggtttc cgatattgga acaagggtac
    12361 aggttgaagt aaatgataat gggattggga ttgaccagaa ggatttaccc cgaatatttg
    12421 aacgctttta cagggttgat aaaagccgtt cgcgcgacca gggtggaacc ggacttggcc
    12481 ttgccattgt aaagcatatc attgaagggc acaagcaggt tattggtgta cgcagtagcc
    12541 taaaccaggg aacaaccttt agctttacgc tcgataaacc taaaaaatag gcagcaccaa
    12601 gctgcgtttt agtactcaat gatttgtttc cgcctatcga gcgaggggta aagctcgtat
    12661 cgcaaagcct gatagtcgat tagttgaccc tctgagttaa aaaccgatat aaacttaagc
    12721 atgcccttat cgttgtactc aagggttgtt cggcttagta gtttccccgc agaatcgaac
    12781 tcttccaccg ctgagcgcag gtttttcttg ttatactcgt atttaaagct tttagcttcc
    12841 ttacggccgt cggcaaaggt ttttgtaata gtttcagcca ccttcctgcc cagggtgtcg
    12901 taagtgggtt tacaacttaa attcactatg gagtcaaccg gaatatagcc ctttgatact
    12961 ttaacctgac gtagtatttc ggttccgtag ttgtcataaa ccgacttggt tcggtaaacc
    13021 cgcttcccct tgctgttgtg ggcatcaacc tgaataagta gcctgttctg gtacttaaag
    13081 ctaagtgtaa cgtaatgaac ggtatcgcca gggtttacca ggtaaacctt ttgggtagct
    13141 actagcgaat cgggtgtata ggtgaaaagg ctgtacctga aaagcttttc gttagcaaat
    13201 tcatcggttc taacaatttg ttccccctca taggtgtgcc aaatgtagcg atacaccccc
    13261 tgggtttcat aatcgcggaa tagaattgta tggactctat cgagtgaatc cttaatctcc
    13321 tgcttaatgg cccggctatt aattgttcgt tttccgtcct ttacctcata gcggctggtt
    13381 actatcacac gccttacatt ctgtggtgga cggttttgag cctcggcatt cactaggaaa
    13441 agcaccagta acagaacaac taaaggggta acttttagcc tcatagcaat cagtttttat
    13501 gttgcaattt aacgaaattt tccctgaaag gttttactct aaaactgttt tgaaaagtat
    13561 aagcagtaaa ctacctattt gttacatttg caaaaatgtt tgagtagatg catgaagatt
    13621 taagacaact tgttgagcaa gcctgggata acagggaact tcttaaccag aaagagtaca
    13681 ttcagggtat cgaggctgtt attgacttac tcgataaggg gcaaatccgt gtagcacaac
    13741 ccattgaaaa gggttggctg gtaaatgaat gggtcaagaa agcggtactg atgtactttc
    13801 ccatacggaa gcttgaggtt atggaggcag ggattctgca attctacgat aaaatacccc
    13861 ttaaaaggga ttttagtgcg attggtgtac gcgcggtgcc gcatgccgta gcccgctatg
    13921 gcagttacct tgccaagggt gtggtgctga tgccctcgta tgttaacatt ggggcatatg
    13981 ttgatgaagg aaccatggtg gatacatggg ccaccgtggg ctcctgcgca caaattggga
    14041 aaggtgtcca cctgagcggt ggcgttggaa ttggcggggt tcttgaaccc gttcaggctg
    14101 caccggtaat agttgaggat ggttgcttta ttggttcgcg ttgtatcata gttgaggggg
    14161 tgcacattgg ccgggaagct gtacttggcg ccaatgttgt gataactcaa tcaacaaaaa
    14221 ttattgatgt gtcgggccct gagcctgttg aataccgcgg atttgttccg gaacgctcag
    14281 tggttatacc gggatcatac acaaagaaat tccccgccgg cgattatcag gtttcgtgcg
    14341 cattaattat tggtaaacgc aaggaatcta ctgacctaaa aacctcgctc aacaacgccc
    14401 tcagggattt tggggtgagt gtttaacnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14521 nnnnnnngac ctaaaaacct cgctcaacaa cgccctcagg gattttgggg tgagtgttta
    14581 acgcaagtac tgtgagtata gagtaatgaa gcggaatgtg taggaatagt ataatttgat
    14641 tgaaattgag tacgttaatt gtaactggga tgttaaattt ttaaggaaat tcgtaaatga
    14701 aaaaagttgt ttagatgttt tcagtatttc cattaatcaa tctcagttta acaaatctta
    14761 aatgtactaa ttccgcctta cctgaaagca gtatctttta agtgaagaaa agtattacct
    14821 ttgtcggtta attatcatgt aaatacagtt ttgatggcag atattgattt tcttaagaaa
    14881 atggcggcac aggttcgccg cgatgttatc cgtatggttc ataaacctgc aagcggtcac
    14941 cccggaggtt cgcttggctg cgccgatttc tttaccgcac tctactttga ggtgatgaac
    15001 atcgatccaa aaaactttac catggatggt actggtgagg atgttttctt tttgtcgaat
    15061 ggtcacctat cggctgcgtg gtacagcgtg cttgcccgcc gtggattttt tgaggttgca
    15121 gagctatcaa cattccgatt aatcaactca cgtttacagg gccaccccac cactgctgaa
    15181 cacttacctg gaattcgcgt ggcttcggga tcgttgggac aaggcctttc ggttgcatgt
    15241 ggtgctgctc tggcaaaaaa gcttaatggc gataaaaatc tggtttacac cctgcacggc
    15301 gatggcgagc tgcaggaggg tcagatttgg gaagcagccc tttttgccgc tgccaaaaag
    15361 attgataacc ttattgctac tgttgactac aacggaaaac aaattgacgg cccggttgat
    15421 gaggtggtta cccttggcaa cctaaaggct aaatgggaat cgtttggctg ggaggtgctg
    15481 gaatccaatg gcaacgacat ggagaaactc atttccacct ttgctctcgc taaaagcaaa
    15541 accggccaag gtaagcccat tgtcatactg atgcgaaccg aaatgggtat gggggttgat
    15601 tttatgatgg gaacgcacaa atggcatggc aaggccccca acgacgaaga gtttgcccgt
    15661 gctatggcac aactccctga aacaattggc gatttttagg ctttagttat aaaaaataaa
    15721 aaaagcaaag ctatgcagaa cattgccctc attgcgcacg atgccaaaaa acctgaactt
    15781 gtcaggttcc tgaaagagca tgaagactgg ttaccaggtg taaacctttt ggcaaccggt
    15841 agaactgctg agttcctgga ggaacagggc attaaatgcc gtcaccttag cccggggaaa
    15901 tcgggcgggt ataatgagat tacggaaatg attggtaaag gtgaaattga tatagtgata
    15961 ttcttgcgcg accataaggt ggtgcaaccc caccacgagg atatccgccg tttgctggaa
    16021 gcatgcaatg tgaataacat tccgcttgct accaacaaag ctacagctga actggttatc
    16081 cttggcctta tccgtaagga atccattgag cgaatgaaac gaaatatcga ctaaagaact
    16141 tttcacttta ccagaatcag gttaacccta cctactaggg taatggcttt taatcatttt
    16201 tttacagtta caacgcaaaa gcaaaaaata aataatgaaa tatacttcga ctggaaataa
    16261 ggacacccgt tccggatttg gagccggact acatgagctg ggacgcacta accccaatgt
    16321 ggtagcgcta tgtgccgatc ttattggatc gcttaaaatg gacgatttta taaaggagaa
    16381 tcccaatcgt ttctttcagg ttggaattgc tgaggccaac atgattggac ttgcagccgg
    16441 attaaccgtt ggtggcaaaa ttccatttgc cggcaccttt gccgcttttg caaccggtag
    16501 ggtttacgat caaattcgcc aggttgtagc ctactccaac aaaaacgtta agatttgcgc
    16561 ttcgcacgcc ggccttacgc tgggcgagga cggagcaacg caccaaatgc tggaggatat
    16621 tgggatgatg aaaatgcttc ccaatatgac cgttattgtc ccctgcgact ataaccaaac
    16681 caaagccgca acgctggcca ttgctgacca tgtggggccg gtttaccttc gctttgggcg
    16741 tccggcagta cccaacttca ccccagccga ccagaagttt gaaattggca aggctcagct
    16801 cctggttgag ggtagccatg ttaccatatt tgccaccggc catatggttt ggaaagccct
    16861 tgaggcagcc gatatacttg agcaaaaggg tatctcagcc gaggttatta acatccacac
    16921 cattaagccg ctcgatgagg aagcagttct gaaatccgtt tccaaaacac gctgtgtggt
    16981 aactgccgag gagcacatga tgaatggagg tcttggcgat agcattgcac agcttttagc
    17041 acgcagaatg cccacaccgg ttgaaatggt tgccgttaac gatacctttg gggagagtgg
    17101 caaacctgaa gaactactgg tgaaatacgg gctcgatact cctaatatcg ttgaagctgc
    17161 tcaaaaggta ttggcacgca tgtaaaataa ccatttacga atggataacc ataccgatag
    17221 cgagttactc gtaatgtttc ggaatcccga aacgaaacat tacgctttta acttgctggt
    17281 taggaaatat caggaacgac tatactggca agtccgcaaa attgttatta gccacgacga
    17341 tgccgacgat gttattcaaa atactttcct taaggtatgg ggcgggctgg agaactttcg
    17401 ggaagattcc cagctcttta cctggttata ccgaattgcc accaacgagg cattaacgtt
    17461 tctgaagaag aaacgtacaa aattgctcct ccccattgtt gatgtggaac agcagcttgc
    17521 tcgaaccctt gagggcgatc cctttattga tggcgacagc acagcactta agttacagaa
    17581 ggcactcctt aaactacctg agaaacagcg cttggtcttt aatatgaagt actttgagga
    17641 gatgaagtac gaagaaattt ctgaaatcct tggcacctcc gttggtgcgc ttaaggcttc
    17701 gttccatcat gctgttaaaa aaattgaaaa atttttagag gaggattaaa cctataccta
    17761 aacatactat catagtttta gatagatata gcaatagcgc atttggaagg agaatgatta
    17821 tgaaaagcat ggaggaaaaa ttcaacaaaa agttaccctt taaggtaccg gaaggctact
    17881 ttgataacct acctctaagg gtacaggagc gcattgcagc taccgaaaca cagcatgaac
    17941 caaaaagccg atgggtaacc cttaaatcgc agctggcatt tgctgctggt tttgctttca
    18001 tggctttgct aagctatttg ggttattaca tggctcgccc acttatggct gataaaaaag
    18061 ccgttaaaac cgattacgtt gaaattgtaa gccgaaccat ttatgagttc gacgatattg
    18121 atctttacaa ggccattgaa cagaaaaaga aaaccgatag ccttaaagag gtttacaacg
    18181 aaattcccca caggctatac gttaggagca gcaactgcat aacaattatt gacgaaaaga
    18241 aagaaattaa accatgaggc aactctttag gatactaatc gtttttatat ttatagccct
    18301 gggggcaaag gcttactcac aaaccgacga taccgagaaa gcccttaagg ccgaaaaggt
    18361 tgcttttttg accaaaaagc tcgatttaac ccaggatgaa gccaaaaagt tttggccttt
    18421 atacgatgag tactgggaaa aaaagattaa aatacttaac gaccgtcgca agcttgccga
    18481 tgactttatc caaaacataa acaacataac cgataaggaa gctgttgatt atgccagaag
    18541 gtacgtggag tcccaaaaaa gggaatcgga tctgatagca gagtataaca agctactcct
    18601 tcaaatactc ccacccaaga aggttatgct tctgtaccaa tccaactacg agttcaagaa
    18661 ttacctgctt caaaaagttc aggaaacaaa aaaatagtta taaacatcat aagtttttaa
    18721 ctttttttta catttgtcgg gttacatgtt aattcttttg aggtaatggt aaacgaggag
    18781 aaagtgactg aattagttga tttgtttatt ccggcaacaa ctgaaacacc tgaaattaaa
    18841 tgtgaaaaga gttcagggcg aataatgatg gtgggcaacc tgatccctag cgatccccaa
    18901 tcatttctcg caccaataca tcgttggcta cagagctacg ttacaaccta caccccacaa
    18961 aacataaccg ttgagttcta cctaaccttt ataaacggat gctctgagca tcaccttggg
    19021 aagttactga aataccttga ggagcttcac atcaacggga tgtccgttaa ggttatttgc
    19081 cactacgaga atgaggattt ggacatgaag gaatggggaa aagatctaag catggcactc
    19141 cgaatcccca ttgagctggt tgaaatcaac taagaagtca gcattagtat aatacgttac
    19201 agcaaagctc aaccgctttg ctgtttttcg ttccaatgcg gaaactgctt atatttgcta
    19261 aaaaaataga aaaatgtcaa ttaacgagat tcaggacgca atagttgagg aattcagcgt
    19321 tttcgacgac tggatggatc gctaccaaca gctcatagag tatggcaagg acttgccgcc
    19381 tatagatgag cgcaagcgga cagaacagta ccttatacag ggctgccaaa gcaaggtttg
    19441 gctcgatgct gagctgcgcg atggaaaaat ctactttact gccgatagcg atgccataat
    19501 caccaaagga attgttgcgc tgctaataaa agttctctcg ggccgcaccc ccgatgagat
    19561 tcttaatgct gagctttact ttatcgacaa aattggctta aaggaaaacc tatcgcctac
    19621 ccgtagcaac ggactagttg caatggtaaa gcaaatgcgt atgtatgccc ttgcctttaa
    19681 ggcaaaagcg gagtgaaccg gttaaacctt atgaaacaaa agctgtttct tataaaaaaa
    19741 tagatatgcg cgacgaactc gaaattcaaa ccgatattgt caaaacactc aagggcattc
    19801 acgaccctga aataccggtt aacatttacg atttagggtt aatttacgag gttgatgttg
    19861 acgataatcg taaggttacc ataaccatga ctcttaccgc tcctaactgc ccgcttgccg
    19921 atcaggtagt tgaagaggtt caggaaaaga tttcaaaaat tgatggggta accaaggtag
    19981 aggtacgctt aacctttgac cctccctggg ataaaagccg tatgagcgac gaagctatgc
    20041 tggaacttgg ctttctgtaa ccatcggtat aaaaatggaa aagaaaagcc aaagccgtgc
    20101 ttactttctg gcatcgtttg tagttctctt ttggggcaca tcggccacag cttttaaaat
    20161 cgggttaagt catacagaac cggttcaact gcttttttgg tcatcgcttt cggccagtat
    20221 aatactgttt gttgttttaa catttcaaaa aaaactaccg ctattaaaat tcactagcaa
    20281 aaaggagata ctttactcgc ttgccgaatc ggttttaaat cctttcctat actaccttgt
    20341 gctttttaag gcctacagcc ttttacctgc tcaggttgct caacccctaa attacatttg
    20401 gccaatagtt ctggttgttt tggcggcatt actactaaag caccctctta ggtttactga
    20461 tataattgca ttactattca gcttgattgg cgttgcgttc atatcgtcgc aaggcaatat
    20521 caatattttt gcaaaatcta atccgctggg agttctactg gcagtaggga gttcggtaat
    20581 ttgggctagc ttttggattg tcaacatgta cgataagcat cgccccgaag aggttaaact
    20641 gtttataagt tttgctttag ctacggttct caccctgctt tacatggctg caatagggga
    20701 tttaccagtt tttaatctga aacctttagc cgcatcggtt tacattggag catttgaaat
    20761 gggcataacg tttgtgcttt ggctaaaagc attaaatagc gcaacaaaca cagcccgttt
    20821 aggcaatttt tcgtatcttg taccgttcgt tgctttagta tttgtgagcc tggtgctccg
    20881 cgaaaagata gtatggacta ctctagtagg acttatagtt attattggtg ccattgtatt
    20941 tcaacaactt ttttctaaaa aggatattaa agatgaaccg atttaaacta gcaatcctcc
    21001 ttttatttgg attagcagca tttacctcgt gcgaggatga cgagcaaaaa gtaaatgtta
    21061 ataaagaggt ttacaacctc atgaaggatt ggtactactg gtacgaccag ttgcccaatg
    21121 ttgatcccaa cagctatcct gaccccgttg agctggtaaa agccatccgg gttaaccctc
    21181 ccgaccgttg gagctacgtt accactaagc aggaacttga tgcctactat aaacaagccg
    21241 catatatagg ctttggattt ggtagtgcat ttacagctga caataagcta attattacat
    21301 ttgttttcaa cacatcgcca ctttatgcca atggtattgg tagaggatgg gaaattgtta
    21361 gtatcgatgg gcaaacccca actcccgata actacacctc accaattggc ccatcggagg
    21421 ttggtgttac taaaaccttt gtgttcaaat cgccctcagg gcaaacgact cagcatactt
    21481 ttagtaaagc ccaaattgaa atgaacacag tgctattgga tagcgtttac accatcaacg
    21541 ggaaaaaggt tggctacttt gtcctaaaag gttttataga gaaaacaaac gatgaattaa
    21601 atgctgtttt ccaaaagttt aagagcgcag gtgttagcga gttggtttgc gatttacgct
    21661 ataatggtgg tgggcaagta aatgtatcgc aatttttagg aaatttaatt ggaggcagca
    21721 ttgcttttgg ccgggttttc gggcagtata tccataacga taaacattcc gacaaaaact
    21781 cccatctata cttttccacc acgcagaact cactcgaatt acaacgtgcc atttttatta
    21841 ctacgagcat cacagcgtcg gccagtgaac ttgtaattaa tgcactcaaa ccattcatgg
    21901 atgttgtgct ggttggcagt aaaactcatg gcaaaccggt tggcatgtac acctttgagt
    21961 ttaaagaccc aagcatcgac tgggcaattg tgccagtatg cttcagcatt cggaatgcca
    22021 ataacgatgg cgattactac gatggcattc ctgttaccat tgaagccgcc gatgacgtac
    22081 tcaccccctt gggggaggtt accgaaagca gcctaaatgc ctctctcaac tatcttggac
    22141 ttggaacaaa agtagtaaca aaacgttatg taacagccca acctataact ggaaagggac
    22201 tttacgaaga aattggtgcc tggtgaccat caatgtaata aataatggta tggcagcatt
    22261 caagttccct tttacttttt tctttatcat taaccttttc tttatccttt atcctttacc
    22321 cataagcagt ttctcattct tatccaagtt cttacttaac cttttcttta tcctttatcc
    22381 tttaccc
//
LOCUS       DAOSAL010000039        22058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_1685_length_22058_cov_50.331000, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000039 DAOSAL010000000
VERSION     DAOSAL010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 22058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22058
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1685_length_22058_cov_50.331000"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3357
                     /gene="glgP"
                     /locus_tag="PK049_06820"
     CDS             <1..3357
                     /gene="glgP"
                     /locus_tag="PK049_06820"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOK61307.1"
                     /translation="AKDSLVIGISGRYEFKNKGIDVFLDALAKLNRENNLEKEVLAFL
                     LIPAGHHGPRKDVFHNLRDGKESDNYIKLDDTHVTHYLNDPESEIILKRIRALGLNNA
                     STDKVKVFWVPCYLNGNDGIFNKPYYDLLIGMDITVFPSYYEPWGYTPLESLAFRVPT
                     ITTTLAGFGDWINKHYNNPKDAIRIIHRTDDNDPEVVEQIALTIDYIMKLDNNKKLNL
                     AHNADEISRVALWENFIDYYFQAYDKALKVVGDRTQMYMEFERQEVLPTVERKFKQYQ
                     PNWVRLIIQKRLPEKLKPLDEISRNLWWSWNTDAKEIFETIDKDLWNECEHNPIQFLE
                     RISLPRFQKLENDTTFVDKLQSVYLKFTSYMMKRYERKGPRIAYFSMEFGLHSSLKLY
                     SGGLGVLAGDYLKEASDRNIDMVGIGLLYRYGYFTQNLSASGQQVAVYDQQNFTQSVA
                     TPVRNENGKWITVKVAFPGRDVFVRVWKVEVGRTDLYLLDTDFEDNQPLDRTITHHLY
                     GGDLENRFKQEMILGIAGIRTLNELGITPDIYHINEGHAAFIGLERLRRYIVEEKLSF
                     PEALELVRASSLFTTHTPVPAGHDAFPEELVRTYMAHYPERLKITWDQFMDLGRMTPG
                     DTTERFSTSHLAIRLSQEVNGVSWLHGEVSRKMFAGMWPCYFPNELHISYVTNGVHFP
                     TWTAREWKAILEDSSDSSELGYTLPNWNAIENLPDNKIWDIRNKLRKRLIKLINKRLN
                     NPNLVRFESPRQVIEIQEKLNDKILTLGFARRFATYKRAWLLFKDQDRLAELVNNPQR
                     PVQIFFAGKAHPHDKAGQDLIKKIIEISKQPRFLGRIIFLQNYDMELARRMVQGVDVW
                     LNTPTRPMEASGTSGQKAVMNGVLHFSVLDGWWVEGYRENAGWALPMEPTYPQEEFQD
                     ELDAELIYTILENEVVPAFYERTADDVPQKWISFIKKSMTQVASNFTTLRMINDYQNR
                     FYNKLYYRFNELSENDFEEAKQISAWKRRITRSWDEIEVIRVKQFDMNREPILLGNEY
                     EAEVVLDLGSLAPDEIGVELVVADLIENQQVTVKRVHEFELAGIEGSRATYRLKLIPV
                     EPGAFECGIRIFPKNPLLPHRMDFCLVRWI"
     gene            3513..4523
                     /locus_tag="PK049_06825"
     CDS             3513..4523
                     /locus_tag="PK049_06825"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HOK61308.1"
                     /translation="MSKHKPNMIIIQRPNQTRRKELALTLGFFDGVHLGHRELIQTVV
                     HDAEIMGLESAVLTFWPHPRLVLHKDPEKLRFLNTLNEKAKIIAKFGIDYFIVQEFSR
                     DFFNLDATDFIKLLVYEYKVKHFVIGADHRFGKGGSGDSDLLNKLSGEMGFTVKVIPS
                     QRIEGIDVSSTKIREALLTGNLELANQMLGYPYLITGAVQSGNQLGRRIGFPTANIRP
                     NDPLKLIPKEGVYAVVVNANDTIYKGMLNIGFRPTVGTDNKQTIEVNIFDFEEDLYNS
                     GIEVALVARLRDEKRFASVELLKEQLVIDKQNALEALKNEKVERYQNLFLTLRPERNN
                     NQ"
     gene            4535..5953
                     /gene="ahcY"
                     /locus_tag="PK049_06830"
     CDS             4535..5953
                     /gene="ahcY"
                     /locus_tag="PK049_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOK61309.1"
                     /translation="MEQVIQATLPYKVKDIALAEWGRKEITIAEKEMPGLMSLRKKYG
                     PHKPLKGARITGSLHMTIQTAVLIETLVELGAQVRWASCNIFSTQDHAAAAIAAAGIP
                     VFAWKGETLEEYWWCTAQALSFPEGKGPNLIVDDGGDATLLVHWGYKAENDKSFLDRV
                     PSSHEEKVILSLLKDILAKEPNKWHNLVKELKGVSEETTTGVHRLYQMLERKELLIPA
                     INVNDSVTKSKFDNLYGCRESLADGIKRATDVMIAGKVVVVCGYGDVGKGCAHSMRSY
                     GARVIVTEIDPICALQAAMEGFEVKTVEDTLDEGNIYVTATGNCDVITLEHMQRMKDQ
                     AIVCNIGHFDNEIQMDRLNSQKDVKRINIKPQVDQYTFPDGHSIFILAEGRLVNLGCA
                     TGHPSFVMSNSFSNQTLAQIELWTKNLPVDVYRLPKHLDEEVARLHLEQIGVKLTQLS
                     PKQAEYIGVKPEGPYKPEHYRY"
     gene            complement(5989..6912)
                     /gene="queG"
                     /locus_tag="PK049_06835"
     CDS             complement(5989..6912)
                     /gene="queG"
                     /locus_tag="PK049_06835"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HOK61310.1"
                     /translation="MVKAEDIISIALGVGFHACGVSRAENLDNLEGVYFKWLHNQYHA
                     TMAYMERNIDLRLDPTMLVPGAKSVISVLLSYNTGDVPVNIQSPRIARYACRVDYHFL
                     MKKMLWELLNRMRKSYGEINGRAFVDSAPVMEREWAVRAGLGWIGKNSNLINRNLGSY
                     VFIGELIVDIEIEPTIHLERDGCGSCTRCMDACPNSAIVAPGTVDARKCISYLTIERK
                     QLIADDEVSNLKGWCFGCDICQEVCPWNSKAVRVNGVDQETHIFSGIKVAELSSLSES
                     EFTYKFGNTPLERAGYEKIMGAIKLITRAKG"
     gene            7000..7215
                     /locus_tag="PK049_06840"
     CDS             7000..7215
                     /locus_tag="PK049_06840"
                     /inference="COORDINATES: protein motif:HMM:NF024672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOK61311.1"
                     /translation="MIEFDIETEYIQLDKLLKACRLVYSGGEAHQLVDEGLVKLNGTV
                     ETRRRAKIRPGDKVQVGSETIVVKTKK"
     gene            7221..8138
                     /locus_tag="PK049_06845"
     CDS             7221..8138
                     /locus_tag="PK049_06845"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HOK61312.1"
                     /translation="MEKRVSFLLLVMLAISVIGCGTKKSNEYANLESISSNRSEAFTE
                     TVVDDIAAPVAKSVAAVDIEPKVIKTANVSIEVADYLKSRQKVDSIVAKHKGFVTNEA
                     FQETDTQKSNSITIRVPAQNFDHLLSDIATIAKRVDYQNIYTQDVTEEYFDVKTRLEN
                     KQEVERTYRKHLREAKNIEDILKIENKLAEIRSEIESAQGRLKYIDNQVNLSTVSLYL
                     YQKLEYKYIPEQLPGFWQRVKEGFHWGWKGFLWFLILLVKLWPLWLVGAIAYFTVILI
                     EKQIKSRKKKEKKAKKLQKKAKQTDETVM"
     gene            8423..8496
                     /locus_tag="PK049_06850"
     tRNA            8423..8496
                     /locus_tag="PK049_06850"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8457..8459,aa:Met,seq:cat)
     assembly_gap    8545..8644
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9410..9811
                     /locus_tag="PK049_06855"
     CDS             9410..9811
                     /locus_tag="PK049_06855"
                     /inference="COORDINATES: protein motif:HMM:NF039344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HOK61313.1"
                     /translation="MKGTVSTNQKVELEYQVNTSPGVLFKRLSTPHGLSEWFANDVNL
                     SGNVFTFIWDGDTKKAELVDKKENKFVKFRWITEQNEAGHHFAFKLTKDELTGEVSLT
                     VIEEVNEEEDIDEVVSLWNYQIGELKRLIGA"
     gene            9883..11361
                     /locus_tag="PK049_06860"
     CDS             9883..11361
                     /locus_tag="PK049_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOK61314.1"
                     /translation="MKTLHRFILKSYIGPLVMTFFITMFVLLMQFLWKYIDDLVGKGL
                     SWNVIAELLLYASAGLLPMALPLATLLASLMTMGNLGENNELLAMKSAGISLPRILTP
                     LIVLTIFISVGAFFFSNNVLPYTNLKISTLLYSVKQQKPELIIKEGVFTNLIEGYSIK
                     VGDKDPNTGLMRRILIYDHSKDEENTTVTYADSGYMKPTADQKFLVATLYSGNSYKEV
                     RDNRKPRYSYKEKLPAQKQTFEKEVIIFELKGFELQRADESFFKDSYQTQNIAQLEKT
                     EDSLSKAFNTQIESFSQHVIRSSIFRNPYWGRRQNDAKVTRSLNVDSLYNTLPPVEKI
                     SALERAMEFARSTKSYVSSSKDEFFYKQKYIARHRIEWHRKFTLAFACFVFFFIGAPL
                     GAIIRKGGLGTPVVISVLFFIIYYIINITGEKFARELYWNPFMGMWISSFILLPLGIF
                     LSYKATTDSVIMNADFYIEKVKKLFSFSTTRKKHTELSPKTE"
     gene            11369..12577
                     /locus_tag="PK049_06865"
     CDS             11369..12577
                     /locus_tag="PK049_06865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOK61315.1"
                     /translation="MRILIISHKPPYPSVDGGTLATLNMCLGLAKAGNNVTVLTLSTQ
                     KHPSSLEIIPNEIKALINFEILHVNIKTTLLGYILNFIFSLRPYNIERFISTPFKRLI
                     RKILLNEYFDVVQLEGLYLYPYISTIRSHFRGTLSLRSHNVEHHIWLKLAENETNKLK
                     RFYFRILAKRLARVESNISSKVDALVAIAEPDRQWFVENNFNKPTITIPAGYLKEEVA
                     EDIEPVDFPSICYIGALDWLPNTEGLNWFINWVWPRIQSEIPEIEFHIAGRNASEELA
                     ERLITERNIIFHGQVANSSTFLSRCPIMVVPLLSGSGIRIKIIEGMYLQRAIVATSMA
                     VKGIDIKHNEHILIADSPDEFAKAVCSLIYNPDLGKKIAHNAMEFAQNNLDAIVLAEK
                     LTQFYKQLEG"
     gene            12574..13755
                     /locus_tag="PK049_06870"
     CDS             12574..13755
                     /locus_tag="PK049_06870"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOK61316.1"
                     /translation="MMLHFLFWLTIGIVLYTYCIYLLVLWILCVKREKNNSPENNISQ
                     LPEVTIVIAAHNEEKYILAKYQNTLALNYPSGKLFQLWSEDGSTDGTKEVLGKLKNIS
                     FISNPERVGKAQSINNAMKLVKTPITVFTDANTYLSKNSIIDLVKHFENPEVGCVSGQ
                     KKIKWDDADALASKGEGIYWSFESFLKKVESCSGSTVSGAGELYAIRTNLFKPLPVNT
                     ILDDFEISASIIKQGYKVKYENGAVASETGSLTVEDEKKRKVRIAAGCFQALGRHTYL
                     LNPFKNTEVAFKFFSHKLLRWVLVPPAIVGAPFLNAAILLLYPDSSIIYLASMLTITF
                     FYLLVAIGYLFRNRSNVHPVVTFPYYGFMMNITMLKGLIIFLTGKQTHIWEKANRKAD
                     I"
     gene            13823..15058
                     /locus_tag="PK049_06875"
     CDS             13823..15058
                     /locus_tag="PK049_06875"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HOK61317.1"
                     /translation="MANNDEIKLNTIEEAIEELRNGRVIIVVDDEDRENEGDFIAAAE
                     LITPEIINFMAKYGRGLICASLPEKRCDELELELMVGRNTALHHTAFTVSVDLIGNGC
                     TTGISAADRAKTIKALVDPNTKPEDLARPGHIFPLRAKEKGVLRRPGHTEAAIDLTQL
                     AGLTPGGVLVEIMNDDGTMARLPQLVQVAQEFNLKIVTIKDLISYRLKFESIVEKGVK
                     VKLPTEFGDFALIPFRQKSNGLEHVALIKGEWEPDEPVLVRVHSSCVTGDIFGSYRCD
                     CGPQLHEAMKMIDNAGKGVLVYLNQEGRGIGLFNKIHAYKLQEEGMDTVDANVELGFQ
                     PDERDYGVGASILREVGVKNMRLITNNPKKMSGLEGYGLHVVENIPLVIPPNPYNEFY
                     LKTKHERMGHINLFNPKKD"
     gene            15065..16045
                     /gene="fmt"
                     /locus_tag="PK049_06880"
     CDS             15065..16045
                     /gene="fmt"
                     /locus_tag="PK049_06880"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004294037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOK61318.1"
                     /translation="MPAKESPRIVYMGTPEFAVEPLRAIIEAGYNVSAVVTVPDKPAG
                     RGLKLQPSPVKVFAEQNHIPVLQPEKLKDPQFIQTLKEINPDIAVVVAFRMLPRDVWQ
                     IPRLGTFNLHASLLPQYRGAAPINWAIINGEKETGITTFLLDEQIDTGNILLQKSVPI
                     GETETAGELHDRLMYQGAGLVVETICMLTAGNINPIPQNQIKISGELKPAPKIFKETC
                     RVDWNNPSQKVFNHIRGLSPYPAAWTELKTKRNNVETIIPIKIYQAEKLDQAQSQRPG
                     MLKTDGKTHLHVFCSQGAIALKTVQQAGKKAMGIEEFLRGWQNTELIEVL"
     gene            complement(16040..16975)
                     /locus_tag="PK049_06885"
     CDS             complement(16040..16975)
                     /locus_tag="PK049_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005819141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HOK61319.1"
                     /translation="MNRILVALFFILTGIHLSVFSQNKISREEYISMYSHIAVNNMKQ
                     FGVPASITLAQAMLESDNGNSTLAVKANNHFGIKCHKDWTGATIYHDDDRKGECFRKY
                     KNPEQSFNDHSLFLRGGKRYAFLFDLTPTDYKAWAHGLKKAGYATNPKYAELLIKIIE
                     DNELYRFDQGITVAIKPPKPVVTDWDNYEIDLYKTRPVFIRNRVKYIVAKDGDTFESL
                     ARELDLMPWQLYRYNDLTRDSVIRPGQELYIQPKRWKADRSNPVHTAEEGETMYKISQ
                     MYGVKLKSLYRKNRMKPGDEPEVGQLIYLRKRKPL"
     gene            complement(16984..17682)
                     /locus_tag="PK049_06890"
     CDS             complement(16984..17682)
                     /locus_tag="PK049_06890"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007557940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOK61320.1"
                     /translation="MINFSSDRILYEDNHFLAVNKNCGEIVQADKTGDESLVETVKRF
                     IKERDSKPGRVFLEVTHRIDRPVSGVVLFAKTSKGLSRINNLFQEGKITKKYWAIVKN
                     QPPVESQTLVHYLLRDNKKNKSFVYHREVHGSKIAKLKYELVGRTENYYLLVIDLITG
                     RHHQIRAQLSKIGCPIRGDLKYGYPRSNPDGGIDLHSRVFAFTHPISGEEIKIVAPPP
                     SEKLWSEFLLLGLE"
     gene            complement(17691..18506)
                     /gene="panB"
                     /locus_tag="PK049_06895"
     CDS             complement(17691..18506)
                     /gene="panB"
                     /locus_tag="PK049_06895"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOK61321.1"
                     /translation="MSSESKVKVVTTHVLQEMKVRGEKIAMLTGYDYSMARILDAAGI
                     DVLLVGDSASNVMAGHISTLPITLDQMIYHASSVVRGVKRALVVVDMPFGTYQGNSKL
                     ALRSAIRIMKESGADAVKLEGGSEIIDSVERILSAGIPVMGHLGLTPQSIHKFGTYVV
                     RAREEAEAKKLVEDAHLLERTGCFAIVLEKIPAALGKQVASELKIPVIGIGAGSGVDG
                     QVLVTHDMLGINQEFSPRFLRRYHNLYAEMLGAFQAYISDVKSLDFPNEREQY"
     gene            18815..20728
                     /gene="dnaK"
                     /locus_tag="PK049_06900"
     CDS             18815..20728
                     /gene="dnaK"
                     /locus_tag="PK049_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOK61322.1"
                     /translation="MSKIIGIDLGTTSSCVAVMEGNEPVVITNSEGKRTTPSVVAFAE
                     NGERKVGDPAKRQAIINPQKTIFSIKRFMGETYDKVAQVEIPRVPYKVVRGDNNTPRV
                     DIDGRLYTPQEISSMILQKMKKTAEDYLGHEVTEAVITVPAYFSDSQRQATKEAGEIA
                     GLKVRRIINEPTAAALAYGLDKKNKDIKVAVYDLGGGTFDISILELGDGVFEVKSTNG
                     DTHLGGDDFDQVIIDWLAEEFLKDEGVDLRKDPMALQRLKEAAEKAKIELSSTTTTEI
                     NLPYIMPVNGIPKHLVKTLTRAQFEKLADHLIQRTVEPCRKALADAGLQPSDIDEVIL
                     VGGSTRIPKVQEVVEKFFNRKPNKSVNPDEVVAVGAAIQGGVLTGEVKDVLLLDVTPL
                     SLGIETLGGVFTKLIEANTTIPTRKTEVFTTAADNQPSVEIHVLQGERPLAKDNKTIG
                     RFHLDGIPPAPRGVPQIEVTFDIDANGILHVTAKDRGTGKEQKIRIEASSGLTEEEIK
                     RMREEAKRNEEADRREKERIEKINAADSLIFQTEKQLKEFGDKLPADKKASIEAALGK
                     LKDAHKSQNLAAIENATNELNAAWQAASEELYKAQAQANAGAGQQANANAGSSSSSGN
                     DQEVTDVDFEEVK"
     gene            20875..21096
                     /locus_tag="PK049_06905"
     CDS             20875..21096
                     /locus_tag="PK049_06905"
                     /inference="COORDINATES: protein motif:HMM:NF037437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="HOK61323.1"
                     /translation="MFNAIEVDRKNLTIMGVKFSDLKTLELTARALGSNMFEGFTPTQ
                     KGIEIIRDYVTGKISLTELVAFAKQKAYV"
     gene            21089..21694
                     /locus_tag="PK049_06910"
     CDS             21089..21694
                     /locus_tag="PK049_06910"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOK61324.1"
                     /translation="MSNSYKYIDPDYSYIDPKTGILRNLQDISDPEVLLFVESSAVTK
                     RLLELYENPIKIKGIDSLFEIHRHLFQDIYIWAGKKRVVEISKDGKQFFPTTYFDNAF
                     RYIDQLIDEYRKIPKNNKRKLAEKLAEILDNVNYLHPFREGNGRAQREFIRLLALEKG
                     LILNLNPPDNISVYERYMKGTVESDVTTLTELIFELITADK"
BASE COUNT         6713 a         4788 c         4622 g         5835 t
ORIGIN      
        1 gcaaaggata gcttagtgat tggcatcagc gggcgttacg agttcaagaa taaaggaatt
       61 gatgttttcc tggatgcgct tgcaaagcta aaccgcgaaa ataaccttga aaaggaggta
      121 cttgcattcc tgttgattcc tgcaggtcat cacggaccca ggaaagatgt attccataat
      181 ctacgcgatg ggaaggaaag cgataactac atcaaacttg atgatacgca cgttacacac
      241 tacctcaacg atcccgaatc cgagataata ctaaaacgga ttcgtgcact gggacttaac
      301 aatgcttcca ccgataaggt taaagtattc tgggtcccct gttacctcaa cggaaacgat
      361 ggtattttta ataagccata ctacgatttg ctcataggga tggatatcac tgttttccca
      421 tcgtactatg aaccctgggg ttatacaccg cttgaaagtt tagcctttag agttcctaca
      481 attaccacaa cccttgcggg ttttggcgat tggatcaata agcattacaa taaccccaag
      541 gatgccatta gaataattca ccgtaccgac gataatgacc ccgaagtggt tgagcaaatt
      601 gctttaacca tcgactacat catgaaactc gacaacaaca agaagttgaa tctagcccat
      661 aatgccgacg agatatcgcg cgttgccctc tgggaaaact ttattgacta ctactttcaa
      721 gcatacgaca aagcccttaa ggttgtaggc gatcgcacac aaatgtacat ggagtttgaa
      781 cggcaagagg ttttgcctac agttgagcgg aaattcaaac agtatcaacc caactgggtt
      841 aggttgataa tccaaaaacg cttaccggaa aaactaaaac ccctcgatga aatttcacgt
      901 aacctatggt ggtcctggaa taccgatgcc aaagaaatat ttgaaacaat tgataaggac
      961 ttgtggaacg aatgcgagca caaccccatt cagttcctgg aaagaataag tttaccccgc
     1021 ttccagaaat tagaaaacga tactacgttt gtcgataaac ttcaatcggt ttacctgaag
     1081 tttacctcat acatgatgaa acgctacgag cgaaaaggcc cccgaattgc atacttcagc
     1141 atggagtttg gcctgcacag ctcacttaaa ctttactcag gaggtcttgg tgtactagca
     1201 ggcgactacc tgaaagaggc ttcggaccgc aatatcgata tggttggtat cggcctttta
     1261 taccggtatg gatatttcac acaaaacctt tccgcttccg gccaacaggt tgctgtttac
     1321 gaccagcaaa actttaccca gagcgttgcc acccctgtgc gtaacgaaaa cggtaagtgg
     1381 atcaccgtta aggtggcatt ccctggacgc gatgtattcg tccgggtttg gaaagttgaa
     1441 gtggggagaa ccgaccttta cctcctggac accgactttg aggataacca gccccttgac
     1501 cgtaccatca ctcaccacct ttacggtggc gacttggaaa accggtttaa gcaggaaatg
     1561 attcttggta tagcgggcat tagaacctta aacgaattag gaattacccc cgatatctac
     1621 cacatcaacg agggtcatgc cgcatttatt gggctagaac gtttacgccg ttacattgta
     1681 gaagaaaaac tatcattccc tgaagctttg gaacttgtta gggcctcgag ccttttcact
     1741 acccatacac ctgttcctgc cggccacgat gccttccctg aagagttagt acgaacctac
     1801 atggctcatt acccagaaag gttaaaaata acctgggatc aattcatgga tttaggccga
     1861 atgacccctg gcgacacaac cgagcgtttt tcaacgagcc atctagccat aaggctctcg
     1921 caagaggtta acggggttag ctggctccat ggcgaggtta gccgtaaaat gtttgccggc
     1981 atgtggccct gctacttccc caatgaactt catataagct atgtaacaaa tggcgttcac
     2041 ttccctacat ggactgcccg tgaatggaaa gcaatactgg aagatagttc tgacagttct
     2101 gaattgggct acacacttcc taactggaac gcaattgaga acttgcccga taataagata
     2161 tgggatattc gaaacaagct aaggaaacgc cttatcaaac taattaacaa gcggttaaat
     2221 aatccgaacc tggttaggtt tgaaagccca cgtcaggtta ttgagataca agaaaagctg
     2281 aacgacaaga tacttaccct aggttttgcc cgccgatttg ccacctacaa aagggcatgg
     2341 ttgctattta aggatcagga taggttagcg gaactagtca acaaccccca acgacctgtt
     2401 caaatctttt ttgctggcaa agcccaccca cacgataaag ccggccagga tttaatcaag
     2461 aagattattg aaatatccaa gcaaccacgt ttccttggca gaataatctt cctgcaaaac
     2521 tacgatatgg aactggcccg acgaatggta caaggtgttg atgtttggct caacaccccc
     2581 acccgtccca tggaagcttc aggaaccagc ggtcaaaaag ccgtaatgaa tggagtgctt
     2641 cactttagcg ttctggatgg atggtgggtt gaaggctacc gcgagaatgc cggttgggca
     2701 ctaccaatgg aacccacata cccccaagag gagttccagg acgaactcga tgctgaactc
     2761 atctatacca tacttgagaa tgaggttgta cccgcattct atgagcgcac cgccgacgat
     2821 gtccctcaaa agtggatttc gtttatcaaa aagtccatga cccaagtggc atcgaacttt
     2881 acaacgctta gaatgataaa cgactaccaa aaccggtttt acaacaagct atactatagg
     2941 tttaatgagc tatccgaaaa cgattttgag gaagccaaac agatttccgc atggaaacgc
     3001 cgcattactc gtagctggga cgagattgaa gtgatacggg ttaaacagtt tgacatgaac
     3061 cgtgaaccca tattgctggg taatgagtat gaggctgaag ttgttctcga tttgggttca
     3121 cttgcccccg atgaaatagg ggtagagctt gtggttgccg accttattga gaaccaacaa
     3181 gtaaccgtta agcgtgttca tgaatttgaa cttgctggaa ttgaaggctc aagagctact
     3241 tacaggttaa aactgatccc cgttgaaccc ggtgcgtttg agtgtggtat tagaattttc
     3301 ccaaaaaacc cactactgcc ccaccgcatg gatttttgct tggttcgatg gatataatgg
     3361 aaaaaccgca ttggggaaaa ggttaatgca agccttttcc ccttaccaaa agtttacgtc
     3421 ggttcgtcaa tgctttttat gctttggtca acaagaaaaa tatcatgaac aataatatta
     3481 ctttgaaaca ataaattttt gcaaattagc atgtgagtaa gcacaagcct aacatgatta
     3541 ttattcaacg tccaaatcaa acgcgccgta aggaattagc acttacctta ggcttttttg
     3601 atggcgttca ccttggacac cgcgagctaa tccaaacagt agttcacgat gccgaaatca
     3661 tgggtttgga atcggctgtg ctaactttct ggccacaccc cagacttgtc ctccataaag
     3721 atcctgagaa acttcgtttc cttaacacgc ttaacgagaa agctaaaata attgccaagt
     3781 ttggaataga ctattttatt gttcaggaat tttcccggga ttttttcaat ttggatgcca
     3841 ccgatttcat caaattgctg gtttacgaat ataaggttaa gcattttgta attggtgccg
     3901 atcatcgctt tggaaaaggt ggatcaggcg attcagacct tttgaataaa ctttctggcg
     3961 aaatgggttt caccgtgaaa gtgatcccat cgcaaagaat tgaaggaatt gatgttagct
     4021 ccacaaagat acgcgaagcg ctcctaactg gaaaccttga actggcaaac cagatgctgg
     4081 gttacccata ccttataacc ggtgctgttc aatccggtaa tcagcttgga cgaagaatcg
     4141 gtttccccac tgccaacatt cgtccaaacg accctcttaa gcttattccc aaggaagggg
     4201 tatatgcagt ggttgttaac gccaacgaca ccatttacaa gggaatgctt aacattggtt
     4261 ttagaccaac agttggtact gacaacaagc aaactattga agtaaacatt ttcgattttg
     4321 aggaagacct ttacaattca ggcattgagg ttgcccttgt cgcccggtta agggacgaaa
     4381 aacgttttgc ctccgttgaa ctcttgaaag agcaacttgt tattgataaa cagaatgccc
     4441 tggaagcctt aaagaacgaa aaagttgaac gttatcaaaa cttattccta actttacggc
     4501 ccgaaaggaa taataaccaa taaaatcaat attaatggaa caagtaattc aggctacctt
     4561 accctacaag gttaaggata tcgcattggc agaatgggga aggaaagaaa tcactattgc
     4621 cgaaaaggaa atgccgggac taatgtcgct tcgtaagaag tacggtcctc acaagcccct
     4681 taaaggggca cgcattaccg gttcgctcca catgaccata caaaccgctg tacttattga
     4741 aacccttgta gaactgggag cacaggttcg ttgggcaagt tgcaacattt tctcaactca
     4801 ggatcatgct gcagcagcca tagcagctgc aggcatacct gtatttgcat ggaaaggaga
     4861 aacacttgaa gagtactggt ggtgtaccgc gcaggctctt tcgttccccg aaggtaaagg
     4921 cccaaacctc attgttgacg atggtggcga tgctacactc cttgtacatt ggggttacaa
     4981 agccgagaac gacaagtcgt tcctcgatag ggtgccatcg tcgcacgaag aaaaggttat
     5041 actctcacta ctcaaagata ttttggctaa agagcctaac aagtggcata acctggtgaa
     5101 ggaacttaaa ggtgtttcgg aagaaactac taccggggtt catcgccttt accaaatgct
     5161 tgagcgtaaa gaactactta tacccgccat aaatgttaac gactcggtta ccaagagtaa
     5221 gttcgacaac ctctatgggt gccgcgaatc gcttgccgat ggcattaagc gggcaaccga
     5281 tgttatgatt gccggtaagg ttgttgtggt atgcggttac ggcgatgtag gaaagggttg
     5341 tgctcacagc atgcgctcct acggcgcccg tgtgattgta actgaaattg atcccatatg
     5401 cgcccttcag gctgccatgg aaggttttga ggtgaaaaca gttgaggaca ctctcgatga
     5461 gggtaatatc tacgttaccg ctacaggcaa ctgcgatgtt attactcttg agcatatgca
     5521 acgcatgaag gatcaagcca ttgtttgcaa tatcggccat tttgacaacg agattcaaat
     5581 ggataggctc aactcccaaa aggatgttaa gcgcatcaat attaagccac aggtggatca
     5641 gtacacattc cccgatggcc actccatttt tatcctggcc gaaggccgac tggttaacct
     5701 tggctgcgcc acaggccatc ccagctttgt gatgagcaac agtttctcca accaaaccct
     5761 tgcacaaatt gagctctgga ctaaaaacct acctgttgat gtgtaccgac ttcccaaaca
     5821 cctcgacgag gaagttgctc gcttgcacct tgagcaaatt ggtgttaaac taacccagtt
     5881 gagcccaaaa caagcggaat atattggtgt taaacccgaa ggaccataca aacccgaaca
     5941 ttaccgatac taaacaatta gaactgccgg gaacctccgg cagtttttct atccttttgc
     6001 tctagtaatt aactttattg ctcccataat cttttcatat cctgcgcgtt caagcggagt
     6061 attgccaaat ttataagtaa attcactctc gctaagactt gaaagttcgg caactttaat
     6121 cccactaaaa atatgggttt cctgatcaac tccattaacc cgcacagcct tgctgttcca
     6181 tgggcaaacc tcctgacaga tgtcgcagcc aaaacaccat ccttttaggt tgctcacctc
     6241 atcatcggca atcaactgtt tacgctcaat agttaagtac gatatgcact tccgcgcatc
     6301 tacagtaccc ggggcaacta tagcactatt ggggcaggcg tccatgcatc gggtacaaga
     6361 gccacaccca tctcgctcca ggtgaatggt tggctcaatt tcaatatcaa caataagttc
     6421 cccaataaaa acgtaggaac ctaagttcct gttaataagg ttcgagttct tcccaatcca
     6481 tcccaaccca gcacgaactg cccactcacg ctccattacc ggcgcagaat caacaaatgc
     6541 tcgtccattt atttcaccat agcttttccg cattctgttc aacaactccc agagcatttt
     6601 tttcatcaga aaatggtaat caaccctgca ggcataacga gcaattcggg gggattgtat
     6661 gttaacaggc acatcgcctg tattgtacga aagtaaaaca cttatcaccg atttcgcccc
     6721 tggaaccaac atggtaggat caagcctaag gtcaatattc cgctccatgt aggccatggt
     6781 tgcatggtac tgattatgca accacttgaa gtatacccct tcaagattat ccaagttttc
     6841 ggcccgactc accccacaag catgaaaacc aacacctagc gcaatactga taatatcttc
     6901 agcctttacc atcggtaagt atttgcaaat acaaagattg ttaaatagaa tcgaaattta
     6961 gcttttttac ttaacttggt aaaaatttga catattagta tgattgagtt tgatattgaa
     7021 acggaatata ttcagctgga taagctactc aaggcgtgcc gccttgttta tagcggtggc
     7081 gaagcccacc agcttgttga cgaaggtctt gttaaactga acggtacagt tgaaacccgg
     7141 cggagagcaa aaataagacc tggcgacaag gttcaggttg gttctgaaac aatagtggta
     7201 aaaacaaaaa aatagatgtt atggaaaaaa gagtatcttt tcttttgctg gtaatgctgg
     7261 caatatcagt tattggctgt ggcacaaaaa agtcaaatga gtacgcaaat ctcgaatcga
     7321 ttagttcaaa tcggagtgaa gcctttaccg aaacggttgt tgatgatatt gcagctcctg
     7381 tagcaaaaag tgttgctgcg gttgatattg agccaaaagt aattaaaaca gccaatgtta
     7441 gcattgaggt agccgattac ctaaaatcgc gccaaaaagt agattcaatt gtggccaagc
     7501 ataaaggctt tgtaactaac gaagcattcc aagaaaccga tacccaaaaa agtaacagca
     7561 taaccattag ggtaccggct caaaactttg accatctact tagcgatatt gctactattg
     7621 ccaaaagagt cgattaccag aatatatata cccaggatgt aaccgaagag tatttcgatg
     7681 ttaaaacacg cttggagaac aagcaggaag ttgaaagaac ctacaggaaa catctgcgcg
     7741 aggcaaagaa cattgaggat attctgaaga ttgagaacaa gttggccgaa atccgttccg
     7801 aaattgaatc ggctcaggga aggttaaagt atatcgacaa tcaggttaac ctaagcaccg
     7861 tgtcgttata tctgtaccag aaattggaat acaaatatat ccctgagcaa ttgcctgggt
     7921 tctggcaaag ggtaaaagaa ggattccatt ggggatggaa aggttttctt tggttcctaa
     7981 tccttctggt taagctttgg ccactctggc tagttggtgc tattgcatat tttacagtta
     8041 ttctaattga aaagcagata aaaagtcgca aaaagaagga aaagaaggct aagaaactgc
     8101 aaaaaaaggc caagcaaacc gatgaaaccg tcatgtaacg ggtaaccctt ttggcttgaa
     8161 atacagcgtt gaattagact gccctgcaga aaaaattgca aataacctta aaactatttt
     8221 gcgaataaaa aatttactta tttttgcagt gctaaaaaaa attgcattac ctgctgaaac
     8281 atgctagaat gctgcatatc gaagcaagtg gcaatttaaa atagtgatgg cgaggtagct
     8341 cagttggtta gagcgtcccg attttatggg gaaggtcgcc ggttcgaaat gtaaacgttc
     8401 ttttttgaaa tataagttag ttggcgaggt agctcagttg gttagagcgc atgattcata
     8461 atcatgaggt cgccggttca atcccggctc tcgctacaaa gtcgaaaagc ccgtaaacag
     8521 tgagtttgcg ggctttttta ttttnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8641 nnnnctcgct acaaagtcga aaagcccgta aacagtgagt ttgcgggctt ttttattttt
     8701 aacctttacc aatagtaacc tagttccccc tgtgggtagc tcagttggtc agagcgtccc
     8761 gatttcatcg ggaaggtcgc cggttcaatc ccggctctcg ctacaaagtc gaaaagcccg
     8821 taaacagtga gtttgcgggc ttttttattt tcaaccttta ccaataataa cccagttccc
     8881 cctctgggta gctcagttgg ttagagcgtc ccgatttcat cgggaaggtc gccggttcaa
     8941 tcccggctct ctctacaaag ccgaaaagcc cgtaaacagt gagtttgcgg gcttttttgt
     9001 ttttaacctt taccaatagt aacctagttc ccccctgtgg gtagctcagt tggttagagc
     9061 atcccagact cataggaaag tcgctggtta ggaatgtaaa cgttcttatt tgagatatta
     9121 gttagttgac gaggtagctc agccagtcag agcgtcccga tttcatcggg aaggtcgccg
     9181 gttcaatagg agtcatagag gttataatat cacttacttt aacatcaagg tacttggcca
     9241 caacggattg cgccctctta aataggctaa caatttcttt gtttattaac aacctattgt
     9301 taataaatac cttcaaaaca ttggcataag tattgcattt tatttttaaa ttgcttatgc
     9361 taagttcaag aattggtatg agcagagcgt aaatttaaaa cattcaagta tgaaagggac
     9421 agtaagtact aatcaaaagg ttgaacttga gtatcaggtt aacacctctc ctggtgtgct
     9481 ttttaaaagg ttaagcaccc cccatggttt atcggaatgg tttgctaacg atgtaaacct
     9541 ttcagggaat gtgttcactt tcatctggga cggagatacc aaaaaagctg aattggttga
     9601 taaaaaggaa aataagtttg ttaagtttcg atggattact gagcagaatg aggctggcca
     9661 ccattttgct tttaaactta caaaagatga actcaccggc gaggtatcgc ttaccgtaat
     9721 tgaagaggtt aatgaggagg aggatatcga tgaggttgtt tcactctgga actaccaaat
     9781 tggtgaactc aaacggctga ttggggcata agctaaatat aacatctatc actttcaagg
     9841 aaaaacatta catttgtgat gtttttcctt aatttttaat ccgtgaaaac acttcatcgt
     9901 tttatcctga aatcgtatat tggcccgcta gtaatgacat ttttcattac tatgttcgta
     9961 ctgctcatgc agtttctctg gaagtatatc gacgatttgg ttgggaaagg gctgagctgg
    10021 aatgttattg cagagcttct tttatacgca tcggcaggac ttttgcccat ggcattgcca
    10081 cttgcaaccc tactcgcctc gcttatgacc atgggcaacc taggggagaa taatgagctg
    10141 cttgccatga aatcggcagg aatctcacta ccccggatac ttactccact tatagtactt
    10201 accattttta ttagcgttgg cgcattcttt ttctcaaaca atgttctgcc ctacacaaac
    10261 cttaaaatat cgacactgct ctactcggtt aagcaacaaa aaccagaact tataataaag
    10321 gaaggggttt ttaccaacct gattgaagga tacagtataa aagtaggcga caaagaccca
    10381 aataccggat taatgcgccg aatccttatt tacgatcaca gcaaggacga agaaaacact
    10441 accgttacct acgccgattc cggttacatg aaacccactg ccgatcaaaa gtttttggta
    10501 gccacactct atagcggtaa tagctacaag gaggttaggg ataatcgtaa gccccgttac
    10561 agctataagg aaaaactccc agcccagaag caaacctttg aaaaggaagt aatcatattt
    10621 gaactaaaag gttttgaact tcagcgtgcc gatgagagtt ttttcaagga cagctaccaa
    10681 acccaaaata ttgcccagct agaaaaaacg gaggattcac tttccaaagc atttaacact
    10741 caaatagaat cattctcgca acatgtaatc aggagtagta tcttccgaaa tccatactgg
    10801 ggccgtcgcc aaaacgatgc taaagtcaca cgtagtttga atgttgattc cctttacaat
    10861 acactacctc ccgtagaaaa aatttcggca ctggagcgtg ccatggaatt tgcccgctca
    10921 accaagagct atgtctcatc gtcaaaggat gaatttttct ataaacaaaa gtatattgcc
    10981 aggcatagga ttgaatggca ccgaaaattc acccttgcat ttgcatgctt tgtattcttt
    11041 ttcataggcg caccacttgg ggccataatc aggaaaggcg gattgggtac acctgttgtg
    11101 atttcggttc ttttcttcat tatctactac atcataaaca ttaccggcga aaagtttgct
    11161 cgcgagcttt actggaaccc ctttatgggc atgtggattt catcgtttat tcttcttccc
    11221 ctagggatat tcctctctta caaggcaaca accgattcag tgatcatgaa tgcagatttc
    11281 tacattgaaa aggttaaaaa gttgtttagc tttagcacga caagaaagaa gcataccgaa
    11341 ttatcgccta aaaccgaata aattttaaat gcgaatcctt ataatttcac acaaaccacc
    11401 ctaccccagt gttgacggag gaacactggc aacccttaat atgtgcctag ggctagccaa
    11461 agcaggcaat aatgtaaccg tactaacctt aagtacccaa aagcatccta gctcactgga
    11521 aataattcca aatgagatta aagctcttat taactttgaa atactacacg ttaatattaa
    11581 aacaaccctt ttagggtaca tcctcaactt cattttttcg cttcgcccct ataacattga
    11641 gcgattcata agtaccccct ttaaaaggtt aataagaaaa attcttttga acgagtattt
    11701 tgatgttgtt cagcttgagg gtttatacct ttacccatac attagtacca ttcgcagtca
    11761 ttttagggga accctttcgc ttaggagcca taatgtggag catcatattt ggctaaagct
    11821 tgccgaaaac gagaccaata agctcaaaag gttctatttt aggattcttg ccaaacgttt
    11881 ggccagagtt gaatcgaaca tttcaagtaa ggttgatgcc cttgtagcta tagctgagcc
    11941 cgacaggcaa tggtttgtgg aaaataattt taacaaacca acaattacta tccccgctgg
    12001 gtacttaaag gaggaggttg cagaagatat tgagcctgtt gacttcccat caatatgtta
    12061 cataggcgca ttggattggt tgccaaatac agaaggttta aattggttta tcaactgggt
    12121 ttggccacgt attcaatcgg aaattcctga gatagagttt catattgccg gacgaaacgc
    12181 ctcagaggaa ctggcagaaa gacttattac cgagcgcaac atcatttttc atggccaggt
    12241 agccaattca tctacttttc tgagtagatg cccaataatg gtcgtacctc tactctcagg
    12301 tagtggaatt cggattaaaa ttattgaggg catgtacctg caaagagcca ttgttgccac
    12361 aagtatggct gttaagggta ttgatatcaa gcacaacgag cacattctca tagccgattc
    12421 gcccgatgaa tttgccaaag cggtatgctc gctaatctac aaccccgacc taggaaagaa
    12481 aattgcgcat aacgccatgg aatttgctca aaacaacctc gatgctattg tgctagccga
    12541 aaaacttacc cagttttaca aacaacttga gggatgatgc tccattttct tttctggcta
    12601 acaattggta tagttcttta cacatactgc atatacttac tggtactttg gatattatgt
    12661 gtaaaacggg aaaaaaacaa ttcacctgaa aacaatatta gtcaacttcc ggaagtgacc
    12721 atcgttattg ccgcacataa cgaggaaaaa tacatattag caaaatatca aaacacccta
    12781 gccctgaact atccttccgg aaaattattc cagctctgga gtgaagatgg aagcacagat
    12841 ggtactaaag aagtgctagg taaacttaaa aacatatcat tcatctctaa ccctgaaagg
    12901 gtaggaaaag cgcaatccat taacaatgca atgaagctgg tgaaaacccc aattacagtt
    12961 tttaccgatg ccaatactta tttatccaaa aactcaataa tcgatttagt taagcatttc
    13021 gaaaacccag aagttggatg cgtttcaggt caaaaaaaaa taaaatggga tgacgccgat
    13081 gccttggcat cgaaaggaga aggaatatac tggagtttcg agtcgttttt aaaaaaagtt
    13141 gaaagctgct caggttcaac agtatctgga gcaggcgaac tttatgcaat tcgaacaaac
    13201 ctgtttaaac cattaccagt caatactata ctcgacgact ttgaaatatc ggcaagtatt
    13261 attaaacagg gatataaggt taaatacgaa aatggagcag tagcttcgga aaccggctcc
    13321 cttacggtag aggatgagaa aaaacgaaag gtaagaatag ctgctgggtg ctttcaagca
    13381 ctcggtagac atacctatct acttaatcca tttaaaaata ccgaagtggc ttttaagttt
    13441 ttttcacata aacttttacg atgggttttg gtacctcctg caattgttgg tgcaccgttc
    13501 ttaaatgctg ccattctatt attatatcca gatagttcca ttatctactt agcttccatg
    13561 cttactatta ctttttttta ccttttagta gccataggat accttttccg gaatcgcagc
    13621 aatgtgcatc ccgtggttac cttcccatac tatggtttta tgatgaatat taccatgctt
    13681 aaagggttaa ttattttctt aactggcaaa caaacacata tttgggaaaa ggcaaaccga
    13741 aaagctgaca tttagcaaga tatttttcct ttcaaaatac taattttgcg tcatcctttt
    13801 taatcatttt attggcagca caatggcaaa caacgacgag attaaactaa ataccatcga
    13861 agaggcgatt gaagagcttc gtaatggaag agtaataata gtggttgacg atgaggatag
    13921 ggagaacgag ggcgatttca ttgctgctgc tgagctaatc acacctgaaa taattaactt
    13981 tatggctaag tatgggcgtg ggctaatttg tgcatcactg cccgaaaagc gctgcgatga
    14041 gttggagctt gagctcatgg taggccgtaa caccgctctg caccatacag cctttaccgt
    14101 atcggtcgac cttattggaa acggatgtac aactggtata tcggcagccg atagagccaa
    14161 aaccataaag gcactcgttg accccaatac taaaccggaa gaccttgccc gaccaggtca
    14221 tattttcccg ctacgtgcca aagaaaaggg cgttttacgt cgcccaggcc ataccgaggc
    14281 agctattgac cttacacagc ttgccggcct aacccccggt ggtgtattgg ttgaaataat
    14341 gaacgacgat ggcaccatgg cacgccttcc ccagcttgtt caggttgccc aggagttcaa
    14401 cctgaaaatt gtaaccataa aggacctaat tagctatagg ctgaagtttg aaagtattgt
    14461 tgagaaaggt gttaaggtaa aactccctac ggagtttggc gattttgcac taataccttt
    14521 ccgccagaag tcgaacgggc ttgagcatgt tgccctaatt aaaggcgaat gggaacccga
    14581 tgagcccgtt ctggttcgcg tacactcctc gtgtgttacc ggcgatattt ttggctcgta
    14641 ccgttgcgat tgcggccctc agctccatga ggccatgaag atgattgaca atgcaggtaa
    14701 aggggtgctg gtttacctta accaggaagg gcgtggcata ggccttttca acaagataca
    14761 tgcatataaa ctccaagagg aagggatgga tacagttgat gccaacgtgg aacttggatt
    14821 ccagcccgat gagcgcgact atggtgtggg agcaagtatt ttgcgcgaag ttggtgtaaa
    14881 gaacatgcgg cttattacca acaaccctaa aaagatgagt gggcttgaag ggtatggatt
    14941 acacgtagtt gaaaatattc ctttagtaat accaccaaat ccttacaacg aattttacct
    15001 aaaaactaaa cacgaacgca tgggacacat caaccttttc aaccccaaaa aggactaaaa
    15061 cccaatgcca gcaaaggaat caccccgtat tgtttacatg ggcacgcccg agtttgccgt
    15121 tgaaccactt agggcgatta ttgaagcagg gtacaacgtg tcagctgtag tcactgttcc
    15181 agataaaccc gcaggccgag gtttaaaact tcagccttca ccggttaagg tatttgccga
    15241 gcaaaaccat attcccgtac ttcagccaga aaagctcaag gatccgcagt tcattcaaac
    15301 actgaaagag ataaatcccg atattgccgt tgttgttgcc tttagaatgt taccaaggga
    15361 tgtttggcaa attcctcgtt taggaacctt taaccttcat gcttcgctct taccccagta
    15421 caggggcgct gcacccatta attgggcaat cataaatggt gaaaaggaaa ccggaattac
    15481 cactttttta cttgatgaac agattgatac gggtaatatc cttcttcaaa agagtgtacc
    15541 cataggcgaa accgaaaccg ccggcgaact ccacgatagg ctaatgtatc agggggcagg
    15601 acttgttgtt gaaactatat gcatgcttac tgctggcaac atcaatccaa ttcctcaaaa
    15661 ccaaatcaag ataagtggag agctaaaacc agccccaaaa atttttaagg aaacctgtcg
    15721 tgttgattgg aacaatccat cccaaaaagt tttcaaccac attagggggc ttagcccata
    15781 ccctgctgca tggactgaac ttaaaaccaa aagaaacaat gttgaaacaa ttatacctat
    15841 aaagatttac caggcggaaa aacttgacca ggctcaatca caaaggcctg gaatgttaaa
    15901 aaccgatgga aaaacacacc tccatgtttt ttgttcacag ggtgcaattg cgctaaaaac
    15961 tgttcagcag gcaggtaaaa aagccatggg aattgaagag ttcctaaggg gatggcagaa
    16021 tactgagctt atcgaagtac tataacggtt tacgtttcct caagtaaatt agttgaccca
    16081 cctctggttc atcgcccggt ttcattctat ttttccggta aagtgacttt aacttaacgc
    16141 cgtacatctg cgaaatttta tacatggttt cgccctcttc cgctgtatga accggattcg
    16201 acctatctgc tttccatcgt ttgggctgta tgtatagttc ctgcccagga cgaatcactg
    16261 aatcgcgagt caaatcgttg tacctgtaaa gctgccaggg cattaaatct aactccctag
    16321 ctaacgattc aaaagtatcg ccatccttgg caacaatata ttttaccctg tttcggatga
    16381 aaacaggccg agttttgtaa aggtcaattt cgtaattatc ccaatcggtt acaaccggtt
    16441 tgggtggctt aatggcaacg gtaattccct gatcgaatcg gtaaagctca ttgtcctcaa
    16501 ttatcttaat aagcagctct gcatactttg ggtttgtagc ataaccagct ttcttgagcc
    16561 catgggccca tgccttataa tcggttgggg tcaaatcgaa taggaaagca tagcgctttc
    16621 cgcctctgag aaaaagacta tggtcgttaa acgattgctc cggatttttg tattttctaa
    16681 agcactcccc ttttcggtcg tcatcgtggt aaatggttgc accagtccag tccttatggc
    16741 actttatccc aaagtggtta ttggccttta cggccaatgt gctattccca ttatcgcttt
    16801 ccagcatggc ctgggcaaga gtaatacttg caggtacgcc aaactgcttc atattattaa
    16861 cggcaatatg cgaatacatg ctaatgtatt cctcgcgcga aatcttgttt tgagaaaata
    16921 cagatagatg aatccctgta aggataaaaa acagtgcaac taaaatccta ttcatagcta
    16981 aaactactcc aagcctaaca acaaaaactc actccataac ttctcgctgg gaggaggagc
    17041 tacaattttt atttcttccc cagaaattgg atgggtaaat gcaaaaaccc ttgaatgcag
    17101 atcgataccg ccatcggggt tagaacgggg atagccatat tttaaatcgc ctcgaattgg
    17161 acatccaatt ttcgagagct gagcacgtat ttggtgatgc ctaccggtaa tcaaatctat
    17221 tactaacagg tagtaatttt cagttctacc aacaagctca tacttaagct tggcaatttt
    17281 tgagccgtgc acctcacggt gatatacaaa cgacttattt tttttgttat cgcgcaaaag
    17341 gtaatgaaca agggtttgcg attcaacagg gggttggttc tttacaatag cccaatactt
    17401 tttggtgatt ttcccttcct gaaaaagatt attgatacgg cttaaccctt tgctggtttt
    17461 ggcaaaaagc actactccac tcacaggcct atcaatccgg tgggtcactt ccaggaatac
    17521 gcgacctggc ttactgtcgc gctctttaat aaaccgcttt accgtttcaa ccaacgactc
    17581 atcgccggtt ttgtcggcct gcacaatctc accacagttc ttatttaccg ctaaaaaatg
    17641 gttatcctcg taaaggattc tgtcgcttga aaaatttatc atcttcagta ttagtattgc
    17701 tcacgttcat tgggaaaatc gagcgatttc acatcgctaa tataggcttg aaatgctcca
    17761 agcatttcag cgtaaaggtt gtggtaccgc ctgagaaagc gtggtgagaa ctcctggtta
    17821 ataccaagca tatcgtgggt aactagcact tgaccatcaa ctccactgcc agcaccaatt
    17881 ccaattacgg gtattttcag ctccgaggca acctgttttc ctaatgcggc gggaattttt
    17941 tcaagcacaa tggcaaaaca gcctgttctt tccagcaaat gtgcatcttc aaccagtttc
    18001 ttggcctcag cttcctcgcg tgcccttaca acgtaagttc caaacttatg gattgactga
    18061 ggggtaagcc ctagatgccc cataaccggt atccctgcag ataggattcg ctccacggag
    18121 tcaataatct ccgagccacc ctcgagctta acggcatcgg ctccgctctc cttcataatt
    18181 cgaatggccg aacgtaatgc taatttggag ttgccctggt aggttccaaa gggcatatca
    18241 acaacaacca atgcacgctt tactccacgt accactgaac tggcatggta aatcatctgg
    18301 tcaagggtga tagggagtgt tgatatatgg cctgccatta cattcgaagc agagtcgcct
    18361 accagcaata cgtcaattcc agcggcatcg agtatccggg ccattgaata gtcgtaccct
    18421 gtcagcattg caattttttc acccctgacc ttcatttcct gaagcacatg ggtggttacc
    18481 actttaactt tactctcgct cgacataagt attaaacttt aaggattgtc aaaggtaatc
    18541 tattattcag gaaaacctaa gctaatttat taaaattgaa aaaactgaca aaaagacaca
    18601 attaatgact actatctgaa aacctgaacc ttataaatat atcaactcaa atataaacac
    18661 agaaacctga caaaggatta ctgaaaaact aaaaattttg tgacagattt tcgtctttca
    18721 gtccattttt ggtgttggca caatgattga acaaagtgaa aggaatgttt aaaaacgaaa
    18781 caaatcaaaa gtataacaat aaaaatctaa agcaatgagc aaaataattg gaattgacct
    18841 aggaacaacc agctcatgcg ttgccgtaat ggaaggtaac gaaccggttg ttataacaaa
    18901 cagtgagggg aaacgcacta ccccatcggt agttgcattt gccgaaaacg gtgagcgcaa
    18961 ggttggcgac ccagcaaagc gtcaggcaat aatcaacccc caaaaaacca tattctcaat
    19021 taagcgattc atgggcgaaa cctacgataa ggttgcccag gttgaaatac cccgtgtacc
    19081 ctacaaggtg gttcgtggcg ataataacac cccacgtgta gatattgatg gccgcctgta
    19141 cacccctcag gaaatatcat cgatgattct tcagaagatg aagaaaactg ctgaggatta
    19201 cctgggacac gaagtaaccg aagccgttat caccgtacca gcttacttta gcgactcgca
    19261 acgtcaggct accaaagaag ctggagaaat tgcaggttta aaggttcgtc gtatcatcaa
    19321 cgagcccact gccgcagcac tggcatatgg tctcgataaa aagaataagg atatcaaggt
    19381 ggcagtgtat gaccttggtg gtggtacttt cgatatctca atccttgaac ttggcgatgg
    19441 agtttttgag gttaagtcaa ccaatggcga tacccacctt gggggcgatg actttgacca
    19501 agtaattatt gattggctgg ctgaagagtt cctgaaagat gaaggcgttg acctccgtaa
    19561 agaccctatg gcactccaac gcttaaagga ggctgcagaa aaggctaaga ttgagctctc
    19621 aagcaccaca actaccgaaa tcaaccttcc gtacatcatg cccgttaacg gcatacccaa
    19681 acacctggtt aaaaccttaa cccgagccca gtttgagaaa ctggccgacc acctcattca
    19741 gcgtaccgtt gaaccatgcc gtaaagcttt agccgatgca ggcttacagc cttccgatat
    19801 cgacgaggta atcctagttg gtggttcaac ccgtatccct aaggttcaag aggtagttga
    19861 aaaattcttc aaccgcaaac ctaataagag cgtaaacccc gatgaagttg ttgccgttgg
    19921 tgcagccatc cagggtggcg tgcttaccgg tgaggtaaag gatgttctgc tgcttgacgt
    19981 tacacctctt tcgttgggta tcgaaacctt aggtggtgtt ttcaccaagc tgattgaagc
    20041 taataccacc attcctaccc gtaaaaccga agtatttaca accgctgccg ataatcagcc
    20101 ttccgttgaa atacatgtgc tccaagggga acgtcccttg gctaaggaca acaaaacaat
    20161 tggacgtttc cacctcgatg gaataccacc tgcaccacgt ggcgtacccc aaattgaggt
    20221 tactttcgat atcgacgcaa atggtatttt acatgtaacc gctaaggata gaggtacagg
    20281 caaggagcaa aagatacgta ttgaggcctc atcgggtctt accgaagagg aaatcaagcg
    20341 catgcgggag gaagccaagc gtaacgagga agccgaccgt agggagaaag agcgtatcga
    20401 gaagattaac gctgccgaca gcttgatttt ccaaaccgaa aagcaactca aagagtttgg
    20461 cgataaactc cctgccgaca agaaagcttc cattgaagca gcacttggta aacttaagga
    20521 cgctcataag agccagaatt tagctgccat tgagaacgca accaacgaac tcaatgctgc
    20581 atggcaggct gcatcggagg aactttacaa agcgcaagca caagctaacg ctggtgctgg
    20641 ccaacaggcc aatgccaacg caggtagctc gtcatcatcg ggcaacgacc aggaagtaac
    20701 cgatgttgat tttgaagagg tgaaataacc cgaatggata ctaaccaaag ccgcctaatt
    20761 ggcggctttt tatttatttt atactgtcct atagaccctt ctaataacac tataaaaata
    20821 aagatttgac gaagattttt tgtaaatttg aagaaattta agaactgaaa agagatgttc
    20881 aatgcaattg aagtagacag aaagaatctg acaataatgg gggtaaaatt ttcggacctg
    20941 aaaactttag aactcacagc aagggctttg gggagtaaca tgtttgaagg ttttacacca
    21001 actcaaaaag gaattgaaat tattagggat tatgtgactg gaaaaatatc cctaacagaa
    21061 cttgtggcat ttgcaaaaca aaaagcctat gtctaattct tacaagtaca tagacccaga
    21121 ctacagctac attgacccca agacaggaat tttaagaaat ttacaggata tatcagaccc
    21181 cgaagtatta ctctttgttg agagtagtgc ggtgactaaa cgacttttgg aactttacga
    21241 gaacccgata aaaattaagg gaattgacag cctttttgaa attcacagac atttgtttca
    21301 agatatttac atttgggctg ggaaaaaacg ggttgttgaa atcagcaaag acggcaaaca
    21361 attcttccca actacttact ttgacaacgc ttttagatat attgaccaac taattgatga
    21421 gtacaggaaa atcccgaaaa acaacaaaag gaaattagca gaaaaattgg cagaaatatt
    21481 agacaatgtt aactatttgc atcccttcag agaaggaaat ggacgagcac aaagagaatt
    21541 tattcggctg ttggcattgg aaaagggctt gatattaaat ctcaacccac cagacaatat
    21601 aagtgtttac gaacgttata tgaaaggaac tgttgaaagc gatgttacaa cgctgactga
    21661 gcttatcttt gaacttatta cagctgataa atgagaaaaa gaaggttaat atcaagtgtt
    21721 ttgtttaatg gtaatatacg aggttaattg aaaatttata ttgcatcaac attttgattt
    21781 gttgacaatt ttaacttata aatcttgcac tttaccataa accaatttgt tatactaacc
    21841 aaaggcattc aatttgcaat agaacttaat aaaaaacagt aagcatgaaa cgtatattgg
    21901 cttttctagt tctacttatt ttttctggat tagccattgg gcaaaccgat ggtgcaaaaa
    21961 accaacttga tagtaaaaac cgaaagcagg gttattggtg caagtattac ccaaacggca
    22021 agaaagccta tgagggttgg ttcaaggacg acaaacca
//
LOCUS       DAOSAL010000040        22054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_1686_length_22054_cov_48.763444, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000040 DAOSAL010000000
VERSION     DAOSAL010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 22054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22054
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1686_length_22054_cov_48.763444"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(511..1485)
                     /locus_tag="PK049_06915"
     CDS             complement(511..1485)
                     /locus_tag="PK049_06915"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HOK61325.1"
                     /translation="MQNWVEVISRYNYPDHLKSWWQVINSVLPYFALWVAMYHSLKVS
                     YWLTLALSLIAAGFLVRIFIIFHDCGHGSFFRAERLNRLVGIPLGLLVFTPYHRWHRD
                     HFIHHSTIGNLDKRGIGDVETLTVREYLELTKWGKFKYRIYRHPLFLFAIAPILLFLI
                     QYRIPKSYMNFKQQMYIHLSNIAIVGTIVLLMWWIGWREFILIQLPIIYFASIAGVWL
                     FYVQHQFERVVWKRSHEWDYQSVALEGSSFLKLPKVLQWFSGNIGYHHIHHLSPRIPN
                     YKLQKCHDENEMFSHIKPLSFREILRSFRLRLWDEDRKRLIPFDELQC"
     gene            complement(1687..3147)
                     /locus_tag="PK049_06920"
     CDS             complement(1687..3147)
                     /locus_tag="PK049_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HOK61326.1"
                     /translation="MNKEIFKLEPQRVWKHFHSLTQIPRPSKSEKAVIDFVVKFGNDL
                     GLETIIDEVGNVIIRKPATPGMEDRKGVILQGHLDMVPQKNNDKVHDFEKDPIEAYVD
                     GEWVTANGTTLGADNGIGVAAAMAVLEATDLVHGPVEALFTIDEETGMTGAFGLKPGL
                     LKGDILINMDSEDEGELYVGCAGGLDANIEFNTKLESVPQGTTAYKLAITGLKGGHSG
                     MDINLGRGNSIKLLFRFLYIAEKEFGVRIASIDGGSLRNAIPREAFAVVVVPADKAAA
                     FEKAVKEYESIYKSEFALSEPTLSFTSEKTGNPSTVIDNQTQSKVIRAFFGCPNGVVR
                     MSDSIPGLVETSNNLARVFTDGEKVKGQCLLRSSVDSSKDALAQKMAAIFELAGANIN
                     FTGGYPGWKPNPNSAILKVMQDVYNKKFGKVPEIKAIHAGLECGLLGGVYPNWDMISF
                     GPTIRHPHSPDEKVNIATVQKFWDFLVETLKNIPKK"
     gene            3307..4050
                     /locus_tag="PK049_06925"
     CDS             3307..4050
                     /locus_tag="PK049_06925"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOK61327.1"
                     /translation="MSVVFQVEWYKRVSSTNDVCQQYATKGADEGIVVAAEFQEQGRG
                     QRGNAWESQEGQNLTFSVLLRPNFLPVAEQFYLSKAISLAVSDWLVAYVTNKPVKIKW
                     PNDIYIGDSKVCGILIENSFSSQYLESSVVGVGINLNQVSFPDDLPNPTSLLLETGSR
                     VDPRKALAELVSCIYGRYQQLTEGYRENIDNDYFNAIYRKNQLCKYYSNGEEFYATII
                     GIQPTGELVLRTAEGAIRTFAFKEVTFII"
     gene            complement(4057..4464)
                     /locus_tag="PK049_06930"
     CDS             complement(4057..4464)
                     /locus_tag="PK049_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide cyclase"
                     /protein_id="HOK61328.1"
                     /translation="MTTYESKIVAISKRAEDVFRILSDFRNFSPMAKDKLDDWQATED
                     SCSFKYKGMGPLGVRIIERDEFKTIKFSGDEKSPMEFFMWIQLKEVAPYDTCMKITVK
                     AELNMMMRMMVGNKLKEGIDALADGIAAAFNAV"
     gene            4574..5518
                     /locus_tag="PK049_06935"
     CDS             4574..5518
                     /locus_tag="PK049_06935"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HOK61329.1"
                     /translation="MRIILQPTHDPYFNIATEEYLLKHSNDDYIILYRNTPSVIVGKH
                     QNTMAELNHSFINSSKIPVIRRLSGGGTVFHDLGNVNFCFMVNGREGNLVDFKRFTTP
                     IIEFLKELGLNAYRGEKNDIRIGNYKVSGNAERVHKNRVMHHGTLLYSTNMFWLEQAI
                     KSNENSYTDRAIRSNRSRTINIAELLPHPMTIETFEIALSEFLTKYHSATPLQLDDSA
                     KRTINTLATTKYTDWNWNYGYNASYDFRVKIDSNILVLKVTSGIIEKVEIIQNCSSWI
                     HKLAKLLEGKRHDYAYLKNELGNTEYSEKIQEIIENLF"
     gene            complement(5521..6081)
                     /locus_tag="PK049_06940"
     CDS             complement(5521..6081)
                     /locus_tag="PK049_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61330.1"
                     /translation="MKITGMRFIALLLITFLPIFSIAQETLDTIILVSNRKLICNVKS
                     VSSSKVTILKKDAAAPEELSRKQIHKILYANGRVEKFNDLAFKMIDETNFQSVVITTN
                     PADVDGLYIYGKVESVSGKNSKNAKSAEKNARIRLQRKAAALGANYVLVTKSESRGGY
                     KEVPTHYYEGVAYGFEPPKNENQTPH"
     gene            complement(6148..6483)
                     /locus_tag="PK049_06945"
     CDS             complement(6148..6483)
                     /locus_tag="PK049_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61331.1"
                     /translation="MKPLKFLDKIAIWMLRLTFAGYLILANIGYFRSIAISDFQFYIA
                     LAVVVLAVLFIVGGFTSNQGLTVISSIGIFLLLLYKALTPWPPTLTDHFLVQIVLASA
                     ALVFASRGN"
     gene            6790..7575
                     /locus_tag="PK049_06950"
     CDS             6790..7575
                     /locus_tag="PK049_06950"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HOK61332.1"
                     /translation="MKGAKHILMLIVSIFLWLFVAITAFLLFLIDFVVWLLTFWWDRR
                     LKLLHKYSSVWAMFYIWINPGWRIKVSGLEKIDKSKSYVIVCNHQSAFDIVLLYRLFT
                     HFKWVAKKELAKVPVIGWNLILNNSILIDRASAVSTKKMISQGLKHLKGGSSVLIFPE
                     GTRTKDGQVKRFKEGAFLLAKQANSPILPVVIEGSKDIIPKPGIVKLRQNLTLKVLDP
                     IEVEEVLSTSVPDLTKRLNQLIMEEHKKLAPEKYMNSDNKQVK"
     gene            7674..7985
                     /gene="rplU"
                     /locus_tag="PK049_06955"
     CDS             7674..7985
                     /gene="rplU"
                     /locus_tag="PK049_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOK61333.1"
                     /translation="MYAIVDIAGQQFKVEKDQKIYVNLLEGEAGTEVKFNKVLLVDND
                     GKVAVGTPELKNASVTAKILGHVKGEKVIVFKKKRRKGYRVKNGHRQNYTQIQIAEIV
                     A"
     gene            8006..8266
                     /gene="rpmA"
                     /locus_tag="PK049_06960"
     CDS             8006..8266
                     /gene="rpmA"
                     /locus_tag="PK049_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOK61334.1"
                     /translation="MAHKKGVGSSRNGRESHSKRLGVKLFGGQVAKAGNIIVRQRGTK
                     HYPGMNVGMGKDHTLYALIDGVVTFRKKADDKSYVSVLPQES"
     gene            8355..9632
                     /gene="serS"
                     /locus_tag="PK049_06965"
     CDS             8355..9632
                     /gene="serS"
                     /locus_tag="PK049_06965"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018029164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOK61335.1"
                     /translation="MLTLKYIRENTEEVIRRLAVKHFDGSVIIAKIIELDDKRKAIQL
                     QLDNNLAEQNRIAKEIGKLFSEGKSAEANVAKAKSAELKELSKNLSQELKEVEEEQTK
                     LLLQVPNLPHESVPQGVSASDNVVVRSGGSIPKLHEEALPHWDLASKYNIIDFELGVK
                     ITGSGFPVYRGKGAKLQRALINFFLDYNTAAGYEEVEPPHMVNEDSAYATGQLPDKDG
                     QMYHVTADGFYMIPTAEVPVTNIYRDVILHAAMLPIKLTAYTPCFRREAGSYGKDVRG
                     LNRLHQFDKVEIVQIQHPDRSYETLEEMVNHVEGLLMKLELPYRIVKLCGGDMSFTSA
                     LTYDFEVFSAAQHKWLEVSSVSNFESFQANRMMLRFKEEGDKKAQLAHTLNGSSLALP
                     RIVAALLENNQTPHGIKIPKVLQPYCGFDKIDL"
     gene            9846..11411
                     /locus_tag="PK049_06970"
     CDS             9846..11411
                     /locus_tag="PK049_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61336.1"
                     /translation="MKAFQKILVSIMLTFSFVAAYAQMEELPKSDTAGVTGGAVIGAV
                     TIDGKTYQQFALRTDIPIGKFGFGIDIQLLFDENGQIRKDEWDEWQDYIDKIYYIRYG
                     KKDDPFYIRLGGLDYTYLGYANIVNGYSNMIQYPSRRRYGGELSFWIPAERYKGMPVA
                     GGEFFFNDMKELFVESPRKSAMLVGSRIFYRPLRFLEFGVTGVADLNEYNAFEDRDGD
                     GVPNLIDLYPRDKNLATDMDKISLNDWNYLLNDTYWGPTREERYQKLLSLGLVPADTL
                     RMENLFNLNNQTSKMFAYSFDIGIPIARKENFKFDIYSHITQLRGENRTYGWGVAIPG
                     IRMILGTSNVQNFLTFKAEYRRSSKEFLFGYFNNTYELERAQFIGRDSVITKQQKLIA
                     IQQELNGIYASAELNMFGYIVGYAGYQDLTGSDKTHMRSLHGEVRMGETLKKMLHFAD
                     LKGYYIQNNVQDFRQWKTPSSLVGFNIGYNYKGAIVGIDYRWTFQDLDGNGIIKGSAE
                     TIKTISFRTAVTF"
     gene            complement(11453..12898)
                     /locus_tag="PK049_06975"
     CDS             complement(11453..12898)
                     /locus_tag="PK049_06975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter"
                     /protein_id="HOK61337.1"
                     /translation="MSASIQLALVTIGYLLFIIGWGIYQGRKVKNNADYSIAGRNLPG
                     WVAALSERATGESSWALLGLPGAAYAMGIAELWTAIGCVVGIITAWWLLAWRLRDEAE
                     KYSATTFSDYLAARHGEVAKWIRLVSSSTIVFFFFLYVGAQFLGGGKTLDTMFGIKPQ
                     WGMLITALIIIPYTIYGGFQSVVYTDVVQALVMIFTLTVGPIVGFFYLANHPEVYSSS
                     ITIALESASPTHASLFNGLKGAAAGMFLVGGFSWFFGYLGGMPQLTMRFMAIKDARQA
                     KLARNIGITWTVVAYIGAMLLGMIGLAIFGPNALTDREYVMPATIMKIFPPALAALLI
                     TGAIAAMISTADSLLVLSSTELSENIVKPFRRINDERLVLRQSRIITASLAIIALAIA
                     YVSPQKVIFTLVSYVWAGIGCTFSVVILLTLFWKPFHGRAAITAMILGLVFTIVWISS
                     GMDKVVTAKLVNFFFTLIVAVLSTYLIPSKK"
     gene            13021..14031
                     /gene="amrS"
                     /locus_tag="PK049_06980"
     CDS             13021..14031
                     /gene="amrS"
                     /locus_tag="PK049_06980"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOK61338.1"
                     /translation="MEALYYMHLENKAVQCNLCPHECKLTPGKRGICKTRYNDNGEMR
                     LLISGVIASSGLDPIEKKPLFHFYPGSKIYSIGGYGCNLRCSFCQNYEISQYVPDSNS
                     RLRENLPENIVDKALFLPQNIGIAYTYNEPTVFFELMLATARLAKGKDLKNVMISNGF
                     INHKPLLELLDVIDAFNIDLKAFTNEFYKTQAGGKLAPVLNNLKTIKKNGNHLEIAFL
                     VIPELNDSTNEAVEMFRWINENLGNDTPLHINRYYPAYKLSIPPTPPEKLVELRDVAM
                     EYLKFVYIGNVHNSDIGTKTTCPSCNTTVIERHGYSTTLKMIDTRGYCKICGYGPIAV
                     MG"
     gene            complement(14028..14600)
                     /locus_tag="PK049_06985"
     CDS             complement(14028..14600)
                     /locus_tag="PK049_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOK61339.1"
                     /translation="MLRDLVVQNRSYRRFDQSHNIDLGLLEQLVELCRFCPSGRNLQP
                     LKFMIINTPEWCNNLFPNLAWAGYLKDWDGPVEGERPSAYIVVLEDQRLSTASQWDQG
                     IVAQTILLGAVEKGLGGCIIASVKKENLHKLLNLPEYLKVALVIALGKPVEKVVIDDI
                     DDSGSVIYWRDSEQVHHVPKRKLSELIWRG"
     gene            complement(14603..15190)
                     /locus_tag="PK049_06990"
     CDS             complement(14603..15190)
                     /locus_tag="PK049_06990"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOK61340.1"
                     /translation="MKHELPKLQYSLNALAEVISEKTMEFHYGKHHQAYVNNLNNLIP
                     GTKFENASLEQIVMEAEGGVFNNGAQVWNHTFYFEAFSSVPKHKPEGDLLKAIEASFG
                     SFEEFLSKFSNAAATLFGSGWAWLVVDTDGKLNITQESNAGNPLRKGLKPLLTCDVWE
                     HAYYLDYQNRRPDYIQAFWKIVDWSVVEKRFEKVR"
     gene            complement(15202..17640)
                     /locus_tag="PK049_06995"
     CDS             complement(15202..17640)
                     /locus_tag="PK049_06995"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOK61341.1"
                     /translation="MFFNRIHIQNLILSCFITVIFPVLAHGQKMMVDTAQVNSQLATV
                     RSLIDVREFSAAKKIAQKVLANTSASEYNEARAWCEYYLGIIYFEITPIDSAFYWLNR
                     AKADFSLINNQIALIKTYNRLGIFYRRTDLNDSSLHYYNLALNKATAIADSTGLAEAN
                     YGLGMVNAQIGNYTKALEHYSNSLAIREKLNDKAGIASVYNSMGILFWEQGAYSTALD
                     FFFKALPLRVETKDRQGEAFLLNNIGLIFRDVAQYEKSLDYLRKSWKIKIEINDKRGI
                     SNSLMNIGSVYLLLGQVDSALLYFEDALALKQILLDRGGVANINRFLGEAYMKIKMYD
                     KAQLYLTKAIEGYTKLEEPRGVVESKYDYALLLFQMGNSPKAFALVDEVLEQAKKHGM
                     MDMVAQSYNALYQFNLAKNNCQGALQNYEKYIAVKDSIAGTEKMKQILAAQLQVEYEN
                     IVRQHFERFNEQLSLVDSQKQSRTRLLILVAIAFVLLLGLFLGLLYTMYKRKQALVEV
                     ESQRISVDIKQRELQEQRDELERQKNLVVYQRDRIINILTDLGESIEYARKIQQAVFP
                     TDALLSQYFRDFFVLYLPKETVGGDFYWVGNCSGKIGFAVADCTGHGVPGGFMSMLGI
                     SMLNDLVANTPTSSPGLLLGKLRDNIISALRQGGHEDDSHDGMDVAFCTFDRSTHTLN
                     YSGANMPIIISTNSNIPNDNRIVQVGEGLIEFRPDRMPVAYYERMERFNELSIKLEPN
                     DTVYLFSDGFTDQFGGKPSKKFGYHSFRNLILSIKHLPLYEQKMTLYRTLEKWKGEEE
                     AQTDDILILAVKPI"
     gene            complement(17742..18353)
                     /locus_tag="PK049_07000"
     CDS             complement(17742..18353)
                     /locus_tag="PK049_07000"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOK61342.1"
                     /translation="MAFQLPKLKYDYKALEPYIDEMTMDIHYNKHHGAYTNNLNNAIA
                     GTSIEALPIEEILRGVSKHSPAIRNNGGGFFNHNLFWEIMSPNGGGKPNGELLKGIEK
                     FFGSFDEFKEKFSNAAATRFGSGWAWLVWTGKDLQVFSSPNQDNPLMDVSEVKGTPIL
                     GLDVWEHAYYLKYQNRRPEYIQNFWNVIDWKAVEEKYLVATGK"
     gene            complement(18431..18616)
                     /locus_tag="PK049_07005"
     CDS             complement(18431..18616)
                     /locus_tag="PK049_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61343.1"
                     /translation="MKRPFIGKSKHLQDTILSLDHKWYVEFWVIRYYFLGIEVYRTKK
                     EKVRYVNGKNGNSVNSQ"
     gene            complement(18762..19586)
                     /locus_tag="PK049_07010"
     CDS             complement(18762..19586)
                     /locus_tag="PK049_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HOK61344.1"
                     /translation="MNLSLNFSTCPNDTFMFYALVHGLLTDKYTFDVHMADIEDLNLL
                     ALEAKPDITKVSYGAYPLIAEHYQLLDSGSALGKGVGPLLISKETIPFEQVKHIRIAL
                     PGEHTTAHLLFNLVFPDVHDKRFYLFSQIEDAILNNEVDAGVIIHESRFTYQFKGLTK
                     LMDLGDEWEKISGLPTPLGGIAIRRSLPDDVKHDINRLLRESVEYAFQYPENTMGFVS
                     QFAQELSTEVMRNHISLYVNDFSITLGQVGRKAIIQLISSSKPTHNFDERSVFVYE"
     gene            complement(19588..20244)
                     /locus_tag="PK049_07015"
     CDS             complement(19588..20244)
                     /locus_tag="PK049_07015"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61345.1"
                     /translation="MKKILIVVASQVEILPQYRQPGNDFYSLQQYGIHADILISGPGA
                     VPTVFALSRAADAYDIIINGGIAGSFTEQLPLGNVVLIDSDSFADYGVDDNGTFRHID
                     NILPPLQKALPLRELVNPYTIKPNPKLFGVRGITVSTVSGSNERIAMIKRSWDAQIET
                     MESAAVFYACLKLGKPFFCLRAVSNYVEPRNRQNWKIDIAVQNLWNELVLLVSYLNTL
                     "
     gene            20372..20791
                     /locus_tag="PK049_07020"
     CDS             20372..20791
                     /locus_tag="PK049_07020"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HOK61346.1"
                     /translation="MPIAYLTRRERFSAAHRLYKQELTDEENFRLFGQCSHPNWHGHN
                     YELFVTIKGEVNQVLGYVVNLKVVSQIIKERIISKLDHKNVNIDVDFMKGVLATTENL
                     AIAIWKELEKPLSDVGAQLHCIKIKETENNFVEYYGD"
     gene            20796..21434
                     /gene="folE"
                     /locus_tag="PK049_07025"
     CDS             20796..21434
                     /gene="folE"
                     /locus_tag="PK049_07025"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HOK61347.1"
                     /translation="MEIVNQNASFNEDEGYSKIERWNPETTEKLAYHYKEILKLIGED
                     VGREGLLQTPVRVAKAIQFLTHGYNIDPHVLVRSAKFHEEYKQMVLVKDIELYSMCEH
                     HMIPFYGKAHVAYIPNGYITGLSKVARVVDAYARRLQVQERLTVQIRDCLQETLNPLG
                     VAVVIEAAHMCMQMRGIQKQNSVTTTSAFTGAFLTHNETREEFIHLIGVKLH"
     gene            complement(21510..>22054)
                     /locus_tag="PK049_07030"
     CDS             complement(21510..>22054)
                     /locus_tag="PK049_07030"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOK61348.1"
                     /translation="GDFGFTNRFSFQTELNFISKGTKGIYGTVEETKRMPAIQIPLLA
                     KYTFSLLGLKRVYANGGVYSTTRIGSGKVIYEDGAVYDDYHWTRFDWGFSVGCGAEYP
                     TKDGIWGLDLRYDLGMVDIYRQMDENTKSRFRSFQFALTYKFDMVDLYFKLRKKNNDE
                     GGDRETGNKGPKGLKVEQRN"
BASE COUNT         6948 a         4611 c         4425 g         6070 t
ORIGIN      
        1 gcgctcccct ccgaaagcgg gtgcaaaagt agaaaagcgt ttttaattac gcaagaggat
       61 tatcaaaaaa aacaagattt ttttaaaaaa agttacatat tgttgactat caatgtcaat
      121 ttatacaaga aaaattaaga acgtgatgtt aaatcaaccc aagtgcccat ggattaagaa
      181 attcagcaag gctaatttct agaggaatga gtcggaatga gtcggaatag acggaatatg
      241 atggagttag aggaagttag aggaagttca attccgtgaa acgtgaaccg tgaaacgtga
      301 accggaagaa ttaacaatat agctgtttta gaggatgtta gaggatattc aaatccgtta
      361 aatgtgaacc gtgaaccgga agaattaaga ctatgacaga gctagaggaa gttagaggga
      421 aaaaaaagtt aaatcaaaaa ccttagatta acaaatttaa ccgcccaaaa ataggaatac
      481 agagacattt ttagagaaat ttcgaactac tcaacattga agttcgtcaa atgggataag
      541 tcttttcctg tcctcatccc ataacctcaa cctgaaagat cttaatatct ccctaaatga
      601 gagaggctta atatgtgaaa acatctcgtt ctcatcgtgg catttctgaa gcttatagtt
      661 aggaatcctt gggcttaggt gatgaatatg atgataccca atgttaccgc taaaccattg
      721 taaaaccttg gggagcttaa ggaatgaact tccctctaga gcaaccgatt gatagtccca
      781 ttcatgggag cgcttccata ctaccctttc aaattgatgt tgaacgtaaa aaagccaaac
      841 acctgcaatt gatgcaaaat agattatggg tagctgtatc aaaataaatt cacgccaacc
      901 tatccaccac atcagtaata caattgtacc aacgatggcg atattggaaa ggtgaatata
      961 catctgttgc ttaaagttca tgtacgactt tggtattctg tactgaataa gaaatagaag
     1021 aattggggca attgcaaaaa ggaaaagcgg atgcctataa attctgtact taaatttacc
     1081 ccactttgta agttcaaggt actccctaac cgttagggtt tcaacatcgc caataccacg
     1141 tttatcgagg ttgccaatgg tagagtgatg aataaagtga tcgcgatgcc agcgatggta
     1201 aggagtaaat accaataacc caaggggtat tcccactaat cgatttaacc tttcagctct
     1261 aaaaaaggaa ccgtgcccac aatcgtgaaa aatgatgaaa attcgaacaa gaaagcctgc
     1321 tgcaatgagg gagagagcta gggtcaacca gtatgatact ttcaaactat ggtacatagc
     1381 cacccaaagc gcaaaatagg gcagcacgga atttattacc tgccaccagc tttttagatg
     1441 gtcgggatag ttgtaccttg aaataacttc aacccagttt tgcattttcc agaatagtaa
     1501 ttcagtataa taatttcaat aaaactaact cctaaatata caagggttat gaaagaaatt
     1561 caagacttac gatacaaaag agcaaggatt tgtaacaaat attaacagtg gataaatatt
     1621 ctcagaaccg atagattaac taataaaaaa ggggctttta gccccttttt attgaaacca
     1681 tactggttat tttttgggga tattcttgag tgtttcaacc aaaaagtccc aaaacttttg
     1741 aacggttgca atgtttacct tctcatcggg tgagtggggg tgacggatgg ttggcccaaa
     1801 cgaaatcata tcccagttag ggtaaacacc acctagcaag ccgcattcta acccggcatg
     1861 gattgcctta atctcgggca ctttgccaaa ttttttattg taaacatcct gcattacctt
     1921 gagtatggct gagtttggat taggtttcca tccagggtaa cccccggtaa aattaatgtt
     1981 agcaccggcg agttcaaaaa tagcagccat tttttgagct aatgcatcct ttgaggaatc
     2041 aactgagctg cgcagcaagc attggccttt tactttttcg ccatcggtga aaacgcgtgc
     2101 aaggttgttg gaggtttcaa ccaaacctgg gattgagtcg ctcattcgaa caacgccatt
     2161 ggggcagcca aaaaaggctc tgattacctt tgattgtgtt tggttgtcaa taactgttga
     2221 aggattccct gttttttccg aggtgaacga aagcgttggt tcacttaaag caaactctga
     2281 tttataaata ctttcatact ctttaacggc tttttcaaat gcagcagctt tatcagcagg
     2341 aaccacaaca acggcaaatg cttcgcgtgg gattgcatta cgcaagctcc cgccatcgat
     2401 tgaggcaatg cgaactccga attccttctc ggcaatgtat aggaagcgga aaaggagttt
     2461 aatggagtta ccacgaccaa ggttaatatc cataccggag tgtccaccct ttagtcctgt
     2521 aatggcaagc ttataggcag tggtaccttg gggtacagac tcaagtttgg tattaaactc
     2581 aatgttggca tccagtccac cagcacaacc cacatagagt tcaccttcgt cctcggagtc
     2641 catgttgata aggatatcac ccttaagcag gccaggtttt aacccaaaag ccccggtcat
     2701 tccagtctcc tcgtcaatgg tgaaaagggc ttcaacaggg ccatgtacta agtcagttgc
     2761 ttccaggaca gccatagcag ccgccacacc tataccgttg tcggctccaa gagttgtacc
     2821 gttggctgtt acccattccc catcaacata agcttcaatt gggtcctttt caaaatcgtg
     2881 aactttatcg ttattctttt ggggtaccat atcgaggtgg ccctgaagaa taacaccttt
     2941 acgatcctcc atgcccggag tagccggctt acggataata acattcccta cttcatcaat
     3001 aatggtttcg agcccaagat cattcccaaa ttttactaca aaatcaatta cagccttctc
     3061 gctttttgat gggcgaggaa tttgcgttaa gctatggaaa tgtttccaaa cacgctgtgg
     3121 ttcaagtttg aagatttctt tattcatatc aacctaattg tttaaatttt taatttgatt
     3181 ggccttaaag ataccaatta ttaaccaaca taacaaactg ctgttttaaa acatcaatct
     3241 atgcccccaa acatgcaact ataccaaaaa atcaacgtaa taaaacgggc ataaaaaata
     3301 attgacgtgt cagtagtttt tcaagttgaa tggtataaac gcgtttcgtc cacaaacgat
     3361 gtttgtcaac agtatgctac caaaggtgca gatgaaggaa tagttgtagc ggctgaattt
     3421 caggagcaag ggcgaggaca gcgaggtaac gcatgggaaa gccaagaggg gcaaaacctc
     3481 acctttagcg ttttacttcg cccaaatttc ctacccgttg ctgaacaatt ctacctttca
     3541 aaagcaatat cgcttgcagt ttccgattgg cttgtagcct atgtgacaaa caaaccagta
     3601 aaaattaaat ggccaaacga tatatatata ggtgatagta aagtttgcgg aatactcatt
     3661 gagaatagct ttagtagtca gtacctggaa agttcagttg ttggtgtggg tataaattta
     3721 aaccaggttt ctttccccga cgatttgcca aatccaactt ctttactact tgaaaccgga
     3781 agcagggttg atccccgtaa agcattagcc gaattggtta gctgcattta tggacggtac
     3841 caacagctta ctgaaggcta cagagaaaac atcgataatg actactttaa tgccatttac
     3901 agaaaaaatc agctttgcaa gtattactcc aatggcgagg agttttatgc tacaataata
     3961 ggcattcagc ccactgggga gttagtccta aggactgcgg aaggtgcaat taggacattt
     4021 gcatttaagg aagtaacctt tataatatag gttaagctat accgcattaa aagcggcagc
     4081 aatgccatcg gcaagggcat cgattccttc cttaagctta ttgccaacca tcatgcgcat
     4141 catcatgtta agctctgcct taacagtgat tttcatacag gtatcgtaag gtgctacctc
     4201 cttaagctga atccacatga agaattccat aggtgatttt tcatcaccag aaaattttat
     4261 agttttaaac tcatcgcgct caatgattct gacacccaaa ggccccatcc ccttgtactt
     4321 gaagctacag ctatcctcag tggcttgcca gtcatcaagc ttatccttgg ccattggcga
     4381 gaaattcctg aaatcagaaa gaatcctaaa aacatcctcg gcccttttac taattgctac
     4441 aatcttactc tcgtaggtag tcattttgtt aatttttggt ttgcaaaaat agtaaactgc
     4501 cgtttaaaaa gaaccaattc aaatttttgt agtttatatt tatattgaat aatttacagg
     4561 ttttaagttt aaaatgagaa taatactaca acctacacac gacccatact ttaacattgc
     4621 tacagaggaa tacctcttaa agcatagcaa tgacgattat attattttat accgtaatac
     4681 cccgtcggtt attgttggaa aacaccagaa tacaatggcc gaacttaacc acagttttat
     4741 caacagtagt aaaattcccg taattcggag gttgtcgggt ggagggacag tatttcacga
     4801 tttagggaat gttaacttct gcttcatggt aaacggccga gagggtaatc tggttgattt
     4861 taaacgcttt acaacaccga ttattgaatt tctgaaagag cttgggttaa atgcctaccg
     4921 cggcgaaaag aatgatatta ggattggcaa ctataaggtt tccggcaatg ccgaacgtgt
     4981 gcataaaaac agggtaatgc accatggaac cctcctgtat agtaccaaca tgttttggct
     5041 ggagcaagcc ataaagtcca atgagaacag ctacaccgac agggcaatac gatcgaaccg
     5101 aagccgaaca ataaacatag cggagcttct tccccatcct atgactattg aaacttttga
     5161 aatcgcccta tcagaatttc ttactaaata ccactctgcc actcccttac aactggacga
     5221 cagcgcaaag agaactataa acacactcgc caccacaaag tataccgatt ggaactggaa
     5281 ctacggctac aatgcaagct atgatttcag ggtaaaaatc gactcaaata ttcttgtttt
     5341 aaaggtaact agtgggatta ttgagaaagt tgaaataatt caaaattgca gtagttggat
     5401 acataagctg gcaaaactac tggaagggaa acgacacgat tacgcttacc tcaagaatga
     5461 attaggtaat actgaatact ctgaaaagat tcaagaaata attgagaatc tattctagcg
     5521 ctagtggggt gtttggtttt cgttcttagg tggctcaaag ccgtaggcaa ccccctcgta
     5581 atagtgagtt ggaacctctt tataaccgcc tctactctcg cttttggtaa ccagaacata
     5641 gtttgcccca agggcagccg cttttcgctg caagcggata cgggcattct tttcagccga
     5701 tttggcattt ttgctatttt tccccgatac agattctact ttcccatata tatagagacc
     5761 atctacgtcg gcaggattag tagttatcac taccgattga aaattggtct catctatcat
     5821 cttaaaggcc aaatcgttaa atttttcgac tctgccattg gcataaagta ttttgtggat
     5881 ttgtttgcgt gaaagctctt caggtgcagc agcatctttt ttaaggatcg ttactttact
     5941 tgaactaacc gattttacat tgcaaataag ttttcggtta cttaccagta taattgtatc
     6001 caaggtttcc tgtgctatgg agaagattgg aagaaaagtt attaggagta atgctataaa
     6061 tcgcatgccc gtaattttca ttttaatgaa ttgactgcaa agttaatgcc atttgatgtt
     6121 ataacaaaaa caaaccatta aaatatatca gtttccacgg ctggcaaata ctagtgccgc
     6181 cgaagccagt actatctgga ctaaaaagtg atcggtgagg gttggaggcc aaggagttag
     6241 tgctttatac agtagtagta aaaagatacc gatagatgat atcactgtta aaccttgatt
     6301 tgaggtgaaa cctcctacaa tgaacaacac agctaaaacc acaactgcta atgcaatata
     6361 gaattggaaa tcggaaatag ctatgcttct gaaatatccg atgtttgcca aaataagata
     6421 ccctgcaaag gttagcctta acatccaaat ggctatcttg tcaagaaatt ttaagggttt
     6481 catataccag tttattaatc tgttagtaat aacgttttac aaatgtatta ataattaaca
     6541 tactaaatac catgcaaaaa gaattattca gttcaaactc catgtaggag taacaaaacc
     6601 atttgcatat tgaacaatgc ttaacctctc aatgtggaaa ttcttaactc acacactgaa
     6661 acgtatggca taggttctaa aataacttaa acggcataga tctcaaatta gtatatcaac
     6721 aaatattgta agaaaacccc acgatcaatt cctgcaaaca attgctattt ttgcaggaaa
     6781 attattgcta tgaaaggagc caaacatatt ctgatgctaa ttgtatccat ttttttatgg
     6841 ttatttgtgg ccattacggc attcctactc tttctaattg attttgtagt atggctctta
     6901 actttctggt gggataggcg tttaaaacta ctgcataaat attcgtcggt ttgggcgatg
     6961 ttttatattt ggattaaccc gggttggaga atcaaagttt cagggttgga gaaaattgac
     7021 aaaagcaaat cctatgttat cgtgtgcaac caccaaagcg ccttcgatat tgtgctgctt
     7081 tacagacttt tcacccattt caagtgggtg gccaaaaagg agttggccaa ggttccagtt
     7141 attgggtgga accttattct taacaatagc atactaattg acagggccag tgcagtgagc
     7201 actaaaaaga tgatttctca ggggctaaaa cacctaaaag gagggagttc cgtactaata
     7261 tttcctgaag gtacaagaac aaaggatggt caggttaagc gatttaagga aggggcgttt
     7321 ctgttggcta agcaagcaaa ctcgcccatt ctacctgtgg ttattgaagg ttcaaaggat
     7381 attatcccaa aaccaggaat tgttaagcta cgccagaact taacattaaa ggttcttgac
     7441 cctattgaag ttgaagaggt tttatcaact tcagtccctg atttgacaaa gcgcctgaac
     7501 cagttaatta tggaagagca taaaaagtta gcccccgaaa agtacatgaa ttccgataac
     7561 aaacaagtta aatgataaaa acacaaaaaa gttttactga ttatttgaca attaattaaa
     7621 cttttatacc tttgcaagcc caaaatattg tcaaacaatt catactaaat acgatgtacg
     7681 ctattgtaga tattgcagga cagcaattta aagtagaaaa agaccaaaag atctacgtga
     7741 acctgcttga gggagaggca ggaacagagg ttaagttcaa caaggtgctg cttgttgaca
     7801 acgatggtaa agtagccgtg ggaactcctg aactaaaaaa cgcttcggtt actgctaaaa
     7861 tccttggcca tgtaaaaggc gaaaaggtta ttgtattcaa gaagaaacgt cgtaaagggt
     7921 atagggtgaa aaatggacac cgccaaaact atactcagat tcagattgca gaaatagtag
     7981 cataacacga aaatattgat tagtcatggc acacaaaaaa ggagttggta gttcccgtaa
     8041 cggacgcgaa tcgcacagca aacgcttagg cgttaagctt tttggtggtc aggttgccaa
     8101 agctggcaac attattgtac gccagagagg aactaagcac tatcccggta tgaatgttgg
     8161 catgggcaag gatcacaccc tttacgcctt aattgacggt gtggtaactt tccgcaaaaa
     8221 agccgacgac aaatcgtacg tatcagtttt accacaagaa tcgtagtttt actaaaattt
     8281 tcataacttt ggtctcctgt gcaatgcctt caagtattga acaggagatt ttgttttatt
     8341 aaaaacccaa cactatgctt accctaaagt atatcagaga aaataccgag gaagttatcc
     8401 gtaggttagc agtaaagcat tttgatggtt ctgtaattat tgccaaaatt atagaacttg
     8461 acgacaaacg gaaggctatt cagcttcagc ttgacaataa ccttgctgag caaaaccgga
     8521 ttgcaaagga aattggaaaa cttttttccg aggggaagtc agctgaggca aacgtagcca
     8581 aggcaaaatc ggctgagctt aaggaactgt ccaaaaacct ctcgcaggaa cttaaagagg
     8641 ttgaggaaga gcaaactaaa cttctgctcc aggtgcctaa cctgccccat gaatcagttc
     8701 cgcagggtgt aagcgcaagc gacaacgttg tagttcgatc tggagggagt attccaaaac
     8761 tacacgagga ggccttgcca cactgggacc tggcttcaaa gtacaatatc atcgattttg
     8821 aacttggcgt caaaataacc ggtagtggtt tcccagtata tagaggtaag ggtgcaaagc
     8881 tccaaagagc cttgattaac tttttccttg actacaatac tgcagccgga tacgaggagg
     8941 tagaaccacc gcacatggtg aacgaggact ctgcttacgc aaccggacag ctccccgata
     9001 aggatgggca aatgtaccat gtaaccgctg acggatttta catgatacct actgcggagg
     9061 ttccagtaac aaacatttac cgcgatgtaa ttttacatgc agcgatgctc cccattaagc
     9121 taaccgctta cactccatgc ttccgccgcg aggcaggttc gtacggaaag gatgttcgtg
     9181 gtttaaacag gttacaccag ttcgataagg tagaaattgt tcagatacag caccccgacc
     9241 gttcatacga aacccttgaa gaaatggtta accacgttga ggggctactt atgaaactgg
     9301 aactccccta ccgaattgtt aagctttgcg gtggcgatat gagtttcact tcagcgctaa
     9361 cctatgactt tgaggttttt tcggcagccc agcacaagtg gcttgaggtt agctcagtat
     9421 cgaactttga atcgtttcag gcaaatcgca tgatgcttcg ctttaaggag gagggcgaca
     9481 agaaagcaca gctggctcat acccttaacg gaagttcgct tgcattgccc cgtattgtgg
     9541 cagcgctact tgagaacaat caaaccccac atggtattaa gattcccaaa gttcttcaac
     9601 cctactgcgg atttgacaag attgatttgt aaagtacgaa ttgcaataaa atcagtcccc
     9661 ttgagaactt tttcaagggg atttttgtta gtgggaataa aatgaagact ggcaaaggag
     9721 aaattgcatt cccatgaaaa aaagtttggc ctgttatttg tgttaaatgg ttttaaaata
     9781 aatttgaaac aattaatatt acccttttgg cactgaaaaa aatgtttaac caaacactat
     9841 tagcaatgaa agcattccaa aaaatcctgg taagcattat gcttacgttt tcatttgtag
     9901 cggcttacgc acaaatggag gaactcccca agagcgacac agcaggtgtt actggtggag
     9961 ccgttatagg agcggttacc attgatggta aaacctatca gcaatttgcg ctccgtaccg
    10021 atatcccaat aggtaagttc ggtttcggca tcgatatcca gctacttttt gacgaaaacg
    10081 gacaaatcag gaaagatgaa tgggatgaat ggcaggacta tatcgataaa atttactaca
    10141 tccgatatgg taaaaaggac gatccgtttt acatccgttt gggcggattg gattacacct
    10201 acttgggtta cgccaacatt gtgaatggct actccaacat gattcagtat ccatcgcgca
    10261 ggcgttatgg tggcgaactg tcgttctgga ttccggctga gcgttacaag ggaatgccgg
    10321 ttgcaggtgg tgaatttttc tttaacgaca tgaaagaact ttttgttgaa tcgccccgca
    10381 agtcggcaat gctggtgggt agccgtattt tctaccgccc gctacgcttc cttgagtttg
    10441 gggttactgg cgttgcagac cttaacgagt acaacgcatt tgaagataga gacggcgatg
    10501 gtgttccaaa cctgattgac ctttacccca gggataaaaa cctggcaacg gatatggata
    10561 aaatatccct aaacgattgg aattacctgc ttaacgacac atactgggga ccaacccggg
    10621 aagagagata ccaaaaactt ctgagtcttg gcctggtacc cgccgatacc ctgaggatgg
    10681 aaaacctttt caacctgaat aaccaaactt caaaaatgtt cgcctacagc ttcgatattg
    10741 gaattcctat tgcccgcaag gaaaacttta agtttgatat ttactcgcat ataacccagc
    10801 tacgagggga gaacagaacc tacgggtggg gcgtagctat acccggtatt aggatgattc
    10861 ttggaaccag caatgttcag aacttcttaa ccttcaaggc tgaataccgt cgttcttcca
    10921 aagagttcct gtttggatac ttcaataaca cctatgagct ggaaagagca cagttcattg
    10981 gaagggatag cgtaataacc aagcaacaga aattaatagc tatccagcag gaacttaatg
    11041 gtatttatgc cagtgctgaa ctgaacatgt ttggatatat tgtaggttat gcaggctacc
    11101 aggatcttac tggatctgac aaaacccaca tgcgttcact tcacggtgag gttcgtatgg
    11161 gcgaaaccct caagaaaatg cttcactttg ctgacctaaa aggatactat atacagaata
    11221 acgttcaaga ttttaggcaa tggaaaaccc caagttcact tgtgggcttt aatattggct
    11281 acaactacaa aggagccatt gttggaattg actatcgatg gacattccaa gaccttgacg
    11341 gaaatggaat cattaagggg agcgctgaaa ccatcaaaac aatcagcttt agaactgctg
    11401 taacgttcta atcaataaaa ccccaaaaaa gggagctgtt cagctccctt ttttatttct
    11461 ttgatggaat taaataggtt gataaaacag ccactatcaa tgtaaagaag aaattaacaa
    11521 gttttgctgt aaccacctta tccattccag agctaatcca tacaatagta aaaactaaac
    11581 ccaaaatcat agccgttatt gctgccctcc catgaaaggg tttccagaaa agggtaagaa
    11641 gtattactac agaaaaggta caaccaatac ccgcccacac gtagcttacc agggtgaata
    11701 tcactttctg gggcgataca taggctattg ccaaagcaat tatggcaagc gaagcggtaa
    11761 taattcgtga ttggcgtaaa acgagccttt cgtcattaat acgcctgaat ggtttaacaa
    11821 tattctcgct tagctcagtt gatgatagca caagcaatga atcggcagta gaaatcattg
    11881 cagcaatagc ccctgttata agtaaggctg caagtgccgg agggaatatt ttcatgattg
    11941 tagcaggcat cacatactcc ctgtcggtaa gggcgttggg gccaaaaatg gctaaaccaa
    12001 tcattccgag caacatagcg ccaatgtagg caacaactgt ccaggtaatg ccaatattac
    12061 gcgcaagctt agcctgcctg gcgtccttaa tggccatgaa cctcatggtt agctggggca
    12121 ttccacctag gtaaccaaag aaccagctaa agccacccac aagaaacata cctgcggctg
    12181 ctccctttaa accattgaat aacgaagcat gggttggact cgcgctctcc agagcaatgg
    12241 taatggagct tgagtatacc tcaggatggt ttgccaggta gaaaaacccg acaattgggc
    12301 caaccgttaa ggtgaaaatc attactaaag cttgaaccac atcggtgtat acaacgctct
    12361 gaaatcctcc gtaaatggta taagggataa tgattagtgc ggtaatgagc attccccatt
    12421 gaggcttaat gccaaacatg gtatctaagg ttttaccgcc tcccaggaat tgagcgccta
    12481 cataaaggaa gaaaaagaaa acaatggttg aactactcac cagccgtatc catttggcaa
    12541 cctcaccatg acgtgcagca agataatcgg aaaaagtggt agccgaatac ttttcggctt
    12601 catcgcgcag gcgccaagcc aaaagccacc aagcggtaat tattcccaca acacaaccaa
    12661 ttgcagtcca caactcagct attcccattg cgtaggcagc acctggtagt cccaacagcg
    12721 cccacgacga ttcgccagtt gccctttcgg aaagggcagc aacccaaccc ggtaggtttc
    12781 ggccggcaat ggagtaatcg gcattattct taacctttcg tccttgataa atcccccacc
    12841 caatgataaa aagtaagtaa ccaatggtta caagtgcaag ttgaatgctg gctgacatag
    12901 taaaaaggtt ttaacattat caccctaaat atattaaata gccgatataa aaaccaatgc
    12961 ttgctgttga ttttcaccta taaaaataac taacttgtga actggcaaaa caagtgaggt
    13021 atggaagcat tatattacat gcacctagag aataaggcgg tacaatgcaa tttatgccct
    13081 catgagtgta aactcacccc cggtaagaga ggtatctgca aaacccgtta taacgataat
    13141 ggcgaaatga gactcctgat ctcaggagta atagcatcgt caggattaga tcccattgag
    13201 aagaagcccc ttttccattt ctaccccggt agcaaaattt actcaattgg tggctatggc
    13261 tgcaacctaa ggtgttcatt ctgccagaac tatgaaatat cgcaatatgt tcctgactca
    13321 aatagccgat taagagaaaa tttgccggaa aatattgttg ataaggctct gtttttacca
    13381 cagaacattg gcatcgccta cacctacaat gaaccaaccg tgttctttga acttatgctt
    13441 gcaacggcac gattggctaa aggcaaagat ttaaaaaatg taatgatatc gaacggattt
    13501 attaaccata aacccctact tgaacttctt gatgttattg atgcatttaa tatcgattta
    13561 aaagctttta ccaatgagtt ttataaaact caagcaggcg gaaagttagc cccagtgcta
    13621 aacaatttaa agaccataaa aaagaatggc aaccatttag agattgcctt cctggttatt
    13681 ccggaactta acgactcaac caatgaagca gtagagatgt ttaggtggat taatgaaaat
    13741 ttgggtaacg acacaccctt acacatcaac aggtattatc ccgcttacaa gttaagcatc
    13801 cctccaaccc ctcctgaaaa attggtagaa cttcgagatg ttgccatgga atacctaaaa
    13861 tttgtatata ttggcaacgt ccacaactcc gatatcggca caaaaacaac atgcccctcg
    13921 tgtaacacca ctgttattga gaggcatgga tatagtacta cattgaaaat gattgacact
    13981 agaggatact gcaagatttg tggatacggc cctatagcag taatgggcta accacgccaa
    14041 atcagttcac ttaactttcg cttgggaaca tggtgtacct gttctgaatc gcgccagtaa
    14101 attacacttc ccgaatcatc aatatcatca ataacaactt tctcaactgg tttgccaagg
    14161 gcaatcacca gtgcgacctt tagatattca ggtagattga gtaatttgtg taaattttct
    14221 tttttaaccg atgcaataat gcacccccca agcccctttt caaccgcacc caaaagaatg
    14281 gtttgcgcca ctataccctg atcccactgc gatgctgttg acaacctctg atcctcaaga
    14341 actacaatat aagccgatgg acgttccccc tcaacagggc catcccaatc cttcaagtaa
    14401 cctgcccatg ccagattagg aaacaaattg ttgcaccatt caggagtatt gataatcata
    14461 aacttaagtg gctgtaggtt acggcccgaa ggacaaaacc tacacagctc aaccagctgt
    14521 tcaagcagcc ctaaatcgat attatgggat tggtcaaacc ttcgatagct gcgattttga
    14581 actacaagat cccttagcat gtttacctaa ccttttcaaa gcgtttttca accacactcc
    14641 agtcaacaat tttccagaaa gcttgaatgt agtcgggtcg acgattctgg tagtcgaggt
    14701 agtaggcatg ctcccacaca tcgcaggtta gtaagggctt tagccctttt cgtaacggat
    14761 taccggcatt tgattcctga gtaatgttaa gtttcccatc ggtatcaacc acaagccaag
    14821 cccaacctga accaaaaagg gtggcagcgg cattggagaa cttgcttaaa aattcttcaa
    14881 atgaaccaaa tgaagcctca atagctttta gtagatcacc ctcgggctta tgctttggaa
    14941 ccgaactaaa agcctcaaag tagaacgtgt gattccaaac ctgtgcgcca ttgttaaata
    15001 caccaccctc agcctccata actatttgct caaggctagc attctcgaac tttgttccgg
    15061 gtatcaggtt attcaggttg ttaacgtaag cctgatggtg cttcccgtaa tggaactcca
    15121 ttgttttctc cgatattacc tcagctaaag cattcagtga gtactgcagc tttggaagtt
    15181 catgtttcat agctatagtt tttaaattgg tttaactgcc aaaatgagta tgtcgtcggt
    15241 ttgcgcctcc tcctcaccct tccatttttc tagtgttcta tacagtgtca tcttttgctc
    15301 gtagagagga agatgcttaa tgcttaaaat aagattacgg aatgaatggt aaccaaattt
    15361 tttactgggt ttaccaccaa actgatcggt aaatccgtcg gagaataggt aaaccgtatc
    15421 gttaggctct aactttattg aaagttcgtt gaatcgctcc atgcgttcat agtaagcaac
    15481 gggcattcta tcgggacgaa actcaataag cccttcgcct acctgaacaa tccgattatc
    15541 atttgggata ttagaatttg tgctgataat aattggcatg ttggctcctg aatagttaag
    15601 ggtatgtgta ctcctatcga aagtacagaa agctacatcc atgccatcat ggctgtcgtc
    15661 ctcgtgtcca ccttgccgta aggcagaaat tatgttatcg cggagtttac cgagtagcaa
    15721 acccggcgat gatgtgggag tgtttgcaac aaggtcgttg agcattgaaa tcccaagcat
    15781 actcatgaag ccaccgggaa cgccatggcc agtacagtca gcaaccgcaa atccaatttt
    15841 accggagcag ttcccgaccc agtaaaaatc gccaccaacg gtctccttag gaagataaag
    15901 cacaaaaaag tccctaaagt actggcttag cagtgcatcg gttgggaaaa ctgcctgctg
    15961 tattttgcgg gcatactcaa tggattctcc caaatccgtt agtatgttaa tgattcgatc
    16021 gcgttggtaa actacaaggt ttttctgcct ttcaagttca tcgcgttgtt cctgaagctc
    16081 acgctgtttg atatctactg aaattcgctg gctctcaacc tcaaccaagg cctgcttgcg
    16141 tttatacata gtatatagca gtcccaggaa caacccgagg agtaaaacaa aagcaatggc
    16201 aaccagtatc aacaggcggg ttctactctg cttttggcta tcaaccaagc taagctgctc
    16261 attgaacctc tcaaaatgct gacgaacaat attttcgtac tcaacctgga gctgagctgc
    16321 gagaatctgc ttcatctttt cagtgcctgc aattgagtcc ttaaccgcaa tgtacttttc
    16381 gtagttctgt aaagccccct gacaattatt tttggctagg ttgaactggt aaagcgcatt
    16441 atacgattgg gccaccatat ccatcatccc gtgtttttta gcctgttcaa ggacctcatc
    16501 gactagtgcg aaagccttag ggctattgcc catttggaaa agtagaagag cgtaatcgta
    16561 ctttgactca accacaccac ggggttcttc gagtttagtg tatccttcaa ttgcttttgt
    16621 aagatatagc tgagccttat cgtacatttt gattttcatg taagcttcgc caagaaaacg
    16681 gttgatattg gcaaccccac ccctatcgag cagaatctgc ttaagcgcaa gagcatcttc
    16741 aaagtaaagc aaggcgctat caacttgccc taaaagcagg taaaccgatc caatattcat
    16801 caatgaattg gaaattcctc gcttatcgtt tatctcaatt tttattttcc acgacttcct
    16861 gagataatca agagactttt catactgagc cacatcgcgg aatattagac ctatgttgtt
    16921 tagtaggaat gcttcgccct gtctgtcctt ggtttcaacc cttaggggta atgctttgaa
    16981 aaagaaatcg agggcagtag aatacgcacc ctgctcccag aaaaggattc ccatgctatt
    17041 atatacggag gcaataccag ctttatcgtt caatttctcg cgtattgcca aagagttaga
    17101 gtaatgttca agggcttttg tatagttacc aatttgagca ttaaccatac ccaaaccata
    17161 gttagcttca gccaaaccgg tagaatcggc aatagccgtt gccttattaa gtgcaagatt
    17221 gtaataatgc aatgagctat cgttcaaatc ggttcttcta taaaatattc ctaagcgatt
    17281 gtatgttttt attaaagcta tttgattgtt gatgaggcta aaatcagctt ttgctctatt
    17341 tagccagtaa aaagcggaat caattggtgt tatttcaaag taaatgattc ccaagtagta
    17401 ctcacaccaa gcacgcgcct cattatattc tgaagcgctt gtattggcta atactttttg
    17461 agcaattttt ttggcagcgc tgaattccct gacatcaatt aacgaccgta cagtagcaag
    17521 ctgagaatta acctgcgctg tatcgaccat cattttttgc ccatgggcta aaacagggaa
    17581 aataaccgtt ataaaacaag acagaataag attttgaata tgaattctat tgaaaaacat
    17641 agcataaata ttaacccaat aaaaatacaa aaaaaaagcg accggtaaaa ccggccgcca
    17701 aaccaaaaca taaaccaaac caaacttaaa ccaataaaaa actatttacc tgtagcaaca
    17761 agatattttt cttccaccgc cttccagtca atgacattcc aaaagttctg tatatactca
    17821 gggcggcggt tttgatactt taagtagtat gcatgctccc aaacatcgag tccgagaatg
    17881 ggagtgcctt taacctctga aacatccatt aaggggttat cctgattggg ggatgaaaag
    17941 acctgaaggt ccttacctgt ccaaaccagc caagcccaac ccgaaccaaa acgggtagct
    18001 gcagcgtttg aaaatttttc cttgaactca tcaaaagagc caaaaaactt ttcaatcccc
    18061 tttagtagct cgccattagg ctttccacct ccatttggcg acataatctc ccaaaaaaga
    18121 ttatgattga agaaaccccc tccgttgttt cggattgctg gtgaatgctt ggaaaccccc
    18181 cgaagtatct cctcaatcgg aagcgcttca attgatgtgc cagcaatggc attgttcaaa
    18241 ttattagtat atgcaccatg gtgtttattg taatgaatat ccatggtcat ttcgtcgata
    18301 taaggctcaa gcgccttgta atcgtatttc aatttaggta actgaaaagc catatgttta
    18361 ctatttaaat tgtttctaaa tagtaaaacg cagcttgata aattttgttc aaaggattat
    18421 taagaaatga ttactgagag ttgacagaat ttccattttt cccattaaca tatcttacct
    18481 tttccttttt ggttcggtaa acctcaattc ctaaaaagta gtagcgaatt acccaaaact
    18541 caacatacca cttatgatcg agtgaaagta ttgtatcttg gaggtgtttt gattttccaa
    18601 taaagggtcg tttcattaaa tctaaagttt cagcttgtcg tatgtaagta cccacaataa
    18661 atttaattta aaattaacat cttttaagtt aatctccaaa cagcaattgg tttctaacag
    18721 taaaaacaaa cgattatctg atttgtttac aagggtttat gttattcgta aacaaaaacg
    18781 ctcctttcgt caaaattatg tgtgggtttg ctgctactta tgagctgtat tatggcctta
    18841 cgtcctactt ggccgagggt tatggagaag tcattaacat ataaactgat atggttgcgc
    18901 attacctcag tgcttagctc ctgggcaaac tgtgaaacaa atcccatagt attttccgga
    18961 tattgaaatg catattcaac actctccctt aacaaccggt taatatcgtg ttttacatca
    19021 tcaggtaaag accgacgaat tgcaataccc ccaagaggcg taggtaatcc agaaattttc
    19081 tcccactcat cgcctaaatc cattagcttg gttaatccct taaattgata ggtaaatcgt
    19141 gattcatgaa ttatgacacc cgcatcaacc tcattgttta gaattgcatc ctcaatctgt
    19201 gagaataggt aaaacctctt atcgtgcaca tcaggaaata ctaggttaaa caaaaggtga
    19261 gcagtggtat gttcaccggg cagggcaata cgtatgtgtt taacctgttc aaatggtatg
    19321 gtttccttgc ttataagcaa tgggccaacc cctttgccca gtgcacttcc cgaatcgagt
    19381 agttggtaat gctcagcaat taaagggtat gcaccatagc taaccttagt aatatcgggt
    19441 ttagcctcaa gggctaaaag gttcaaatcc tcaatatcgg ccatgtgaac gtcaaaggtg
    19501 tatttgtcag taagcaaccc atgcaccagt gcataaaaca taaaagtgtc gtttggacaa
    19561 gttgaaaagt tcagcgatag gttcattcta cagtgtattt aagtaactca caagtaagac
    19621 tagctcattc caaaggtttt gaacagcaat gtcgattttc cagttttggc gattccgggg
    19681 ttcaacataa ttggatacag cccttaggca aaaaaatggt ttaccaagtt ttaagcaagc
    19741 atagaaaacc gcagccgact ccatggtttc aatctgagca tcccatgaac gtttaatcat
    19801 agcgattcgc tcattagaac ccgaaacggt tgaaacagtt attcccctaa ccccaaataa
    19861 cttcgggttt ggcttaatag tgtatgggtt aaccaactct cttaagggta aagccttttg
    19921 caacgggggt aaaatattat cgatatgtcg aaaagtgcca ttatcatcta caccataatc
    19981 ggcaaaagaa tccgaatcaa taagaactac attgcctaat ggtaattgct cagtaaaact
    20041 tccagcaatt cccccgttaa taatgatatc ataagcatcg gctgctctgg aaagggcaaa
    20101 tactgttgga actgctcctg gtccgctaat cagaatatcg gcatgaatac catactgttg
    20161 aagcgagtaa aaatcattac ccggttgcct gtactggggc aaaatttcta cttggctggc
    20221 aacaactatc aaaatttttt tcaaaaactg attacttttt aaaataaata gttaaacaat
    20281 aaagtaaaag tgttgttaaa aaataatggt taaaaataaa taatgatagg tattttaaca
    20341 aaaacaaaat tgtttgtaac tttaaaagaa aatgcccatt gcgtacctga cacgaagaga
    20401 acgatttagc gctgctcaca ggttatacaa gcaagaattg accgatgaag aaaattttag
    20461 gctatttggg cagtgctcac accccaactg gcacggacat aactatgagc tttttgttac
    20521 gattaaaggt gaagtgaacc aagtgctggg ttatgtggta aacctcaaag tggtaagtca
    20581 aatcataaaa gagcggataa ttagcaagct ggatcacaaa aatgtaaata ttgatgtaga
    20641 ctttatgaaa ggagtgcttg ccactaccga aaacctagcc atagcaatat ggaaagaact
    20701 tgaaaaacca ttgagtgacg taggagcaca acttcattgc ataaaaataa aagaaaccga
    20761 gaataacttt gtagaatatt acggagatta aatttatgga gatagtaaat caaaacgcat
    20821 cgttcaacga agatgaaggc tactcaaaga ttgagaggtg gaaccctgag acaaccgaaa
    20881 agctagccta ccactacaag gaaatactta agctaattgg ggaggatgtt ggtcgtgagg
    20941 gattattgca aactcctgta agagtggcaa aagccattca gttcctgacc catggctata
    21001 atattgatcc ccatgtgctt gtaagaagtg caaagttcca cgaggagtac aaacagatgg
    21061 tgctggttaa ggatattgaa ctatactcca tgtgcgaaca ccacatgata ccattctacg
    21121 gaaaagctca cgtggcctac attccaaatg gttacattac gggtcttagc aaggtagcaa
    21181 gggttgttga tgcatacgcg cgcaggttac aggtacagga aaggttaaca gttcaaatac
    21241 gcgattgctt gcaggaaacc ctcaatccgc ttggagttgc cgtagttata gaagcagccc
    21301 atatgtgtat gcagatgcgt ggaattcaaa agcaaaactc tgttaccaca acatcagcat
    21361 ttacgggtgc cttccttacc cacaacgaaa cacgcgagga gttcattcat cttattggag
    21421 ttaagctcca ttagcgtgat gaaccagaat aaaaccaaag ggtgcctcaa aatttgaagc
    21481 accctttact ttatgtgaat ggttaatgac tagttgcgtt gctctacctt aagtcctttt
    21541 ggacccttgt tgccggtttc tctatcacca ccctcgtcgt tattcttttt acgcaacttg
    21601 aagtataggt caaccatatc aaatttatag gttagagcaa actgaaatga acgaaagcgc
    21661 gatttagtat tctcatccat ttgtcggtaa atatcaacca tacccaagtc gtaacgtaaa
    21721 tctaaccccc aaatgccatc cttggttggg tattcggctc cgcaacccac tgaaaatccc
    21781 cagtcgaaac gggtccaatg ataatcgtcg taaactgcac catcctcgta aataacttta
    21841 ccgctaccaa ttcttgtagt agagtaaaca ccaccatttg catatactct ctttaacccc
    21901 agcaagctaa aagtgtactt ggcaagtaag ggaatctgaa ttgcaggcat acgctttgtt
    21961 tcctcaactg tcccatagat tccttttgtt cctttggaaa taaagttcaa ctctgtttga
    22021 aaggaaaacc tattcgtaaa cccaaaatca ccaa
//
LOCUS       DAOSAL010000041        21927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_1696_length_21927_cov_48.417337, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000041 DAOSAL010000000
VERSION     DAOSAL010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 21927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21927
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1696_length_21927_cov_48.417337"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="PK049_07035"
     CDS             <1..246
                     /locus_tag="PK049_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61349.1"
                     /translation="IDEVGRMELYGEVWAELIEHHLTKTKNVLVLTVRRENLMHVVER
                     WNLTDAYVFDINKVGVTDAANSIKNLVLSYHAASSRK"
     gene            complement(208..2259)
                     /locus_tag="PK049_07040"
     CDS             complement(208..2259)
                     /locus_tag="PK049_07040"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOK61350.1"
                     /translation="MENLGFHLPISEPITVFGITLLVIFIVPLVFSKLKLPGIIGLII
                     AGMILGTSGLNILDKERSIELLGNVGLIYLMFLAGLELEVGSFLKNQRKNITFGLLTF
                     TIPFLFGFAICKYFLGFGYLPSLLIASMFSTQTMIAFPIVSRLRLTKTLPVEISIGGT
                     IITDTLVLLIFVIITSLSANSGLNGFLLLIPKILAFVLFIILLLPRFTHWFLRNLSSD
                     QMAQYVFVLATVFTNSAIARYLGIEPIIGAFLAGIVLNRQIPPSAMLMNRIEFIGNAL
                     FIPIFLFYVGMLIDLKAFVSGYESLELSAILISFAIISKWLAAYLTQKIFKLKTLDRQ
                     LIFGLSTAHAAATIAIILVGYKIGIVDINVLNATIVVIFASCVVSAMVTERAARSYAV
                     AFPAVKFKPSDTEKVLIPVANPSNIEHLIEFAGYVSERTKKNPITLLRVVKDETEISK
                     VNDLAAKAEEISHSFGVDTEWAVRVDVNISSGIVRAAKEMLASKIIMGWSGKYVSSRR
                     WIFGTILEGVLADVKQPVYICSLKQSIAQISQVHILIPRNAEFDPDFKNYMKSLERII
                     KKTRQQVTIIVDKIHTENVIAELPPTFSKLKPTVKEIRKWEEGFNHLKSIDSNALGII
                     ISARKGTIAYNSDIQNTLIFTSQIISDKNVVIVYPAIHGTSIIDSRSLLSGTGRVVTE
                     N"
     gene            complement(2350..2931)
                     /locus_tag="PK049_07045"
     CDS             complement(2350..2931)
                     /locus_tag="PK049_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOK61351.1"
                     /translation="MKNDIILAGVGGQGILSIAAAIGLAAVNSNLYIKQSEVHGMSQR
                     GGDVQSHLRISDKPIASDLIPAGKADMIISVEPMEGLRYLPFLAQTGWLVTNVTPFNN
                     IPDYPNINDILSKIKAVKNHIAIDADAIAKEAGSTKASNIVMLGAASLFLDIPFEKIE
                     EGIRQLFGRKGDEVVQLNLKALRLGREFSEKNK"
     gene            complement(2950..4566)
                     /locus_tag="PK049_07050"
     CDS             complement(2950..4566)
                     /locus_tag="PK049_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOK61352.1"
                     /translation="MNNNKLLLLGDEAIAQGAIDGGLSGIYAYPGTPSTEIMEYVQAS
                     KQAAELNIHRQWSANEKTAMEAAIGMSYAGKRAMVCFKHVGLNVAADAFINASITGVN
                     GGLIVVSADDPSMHSSQNEQDSRFYGKFSMIPILEPSNQQEAYDMAYFGFELSERLNV
                     PVLMRITTRMAHSRAGVQRRESRPQNEIRLPSDPRQFVLLPSIARKNYKKLLVSQTAM
                     ELESEKSAYNQMIEGTDKSLGIITTGIAYNYLIENFPDHKCPYPVLKICQYPIPRKQV
                     TELYNSCSKLLILEEGYPLVEEMIKGFLNIGKTIHGRLDGTLPRDGELNPNIVAKALG
                     LTVTEGEPVPEVVKNRPPSLCQGCGHRDVYEDLNEALKEYGTGRVFSDIGCYTLGALP
                     PFNAINSCVDMGASITMAKGAADAGLVPAVAVIGDSTFTHSGMTGLLDAVVENSPITV
                     IISDNSTTGMTGGQHSSATGRIVEICKGIGVHPDHIRVVTPLKKNHEENVKIIKEELA
                     YNGVSVIIPQRECIQTFARRKKAEKKETNE"
     gene            complement(4840..5247)
                     /locus_tag="PK049_07055"
     CDS             complement(4840..5247)
                     /locus_tag="PK049_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOK61353.1"
                     /translation="MIKQIEIVLRPYPQGFHLVTAEIVNQLPELPLVGLLHLFIKHTS
                     AALSINENADPSVRTDLNAFFNRHYPMEPYFNHDLEGDDDMPAHIKTTVVGTSISIPI
                     TNGRLNLGTWQGIYLCEFRKHGGSRKIIATVIE"
     gene            complement(5249..5674)
                     /locus_tag="PK049_07060"
     CDS             complement(5249..5674)
                     /locus_tag="PK049_07060"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61354.1"
                     /translation="MKEILIVSQNNACRSAMAEGWFKYYARGAANVTSAGIEPTQLDL
                     NAAQSMMDAVIDITKHQPKSLNSLTDKEFDIVLVLDNKVAKTASSNLQYKNLYVFHFD
                     SPKAAKSDDSLTRKAYDTLRDDIENWAFDFIHQHIKRLM"
     gene            5796..8252
                     /locus_tag="PK049_07065"
     CDS             5796..8252
                     /locus_tag="PK049_07065"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOK61355.1"
                     /translation="MSVKPPYYIFQCVLVIFITGFASLHAQERRVKISGIVKDTLNMP
                     IPSASVIIVGSSLGVSTDDEGRFSITVTDIDTISIRFSCLGYQTQEMNVKAERGKFLE
                     VILKPSEEHIEGVYVRSQQRKVGNIERIGIDGLKTSPNVSGGLESLVKLLPGVSSSNE
                     LSSQYSVRGGNFDENLVYVNDVEVYRPFLIRSAQQEGLSFINPDMVGAVEFSSGGFTA
                     EFGDKMSSVLNVRYREPKANALRVSAGLLGASATLEGVAIGSSLTYMSGVRYKTSRYI
                     LNSLETKGDYTPSFFDWQGIADYKLSERITIGLLGNVAINRFDFQPRVRETRFGVFNN
                     TLQLKIYYDGQEKDRYLSSMAALITTIRVTPNFNVKVIGSSYYSNERETYDLLGQYFL
                     NELDNSMGSKTYGDSLINVGIGGFLDHARNHINLKVQSLNLLGDMWFGKSLIKLGVQL
                     QREVLSDELLEWGLVDSSGYAIPYTGSQVGLDRFFGAASTIESYRALGFVQADLHFNV
                     GNWQYRTNIGFRVSHWSFNKEILFSPRLALSFNHSNYRSLQYHLSFGYYYQPPFYREI
                     RLASGELNRNIKAQQSIHALAGAEYYFTAWSRPFRMSLEFYNKWLNRLIPYSVDNVRL
                     RYSGENLARGYARGADLKINGELVSGAESWLSLSILKTKEDNLYDSYTDEGGNTVYPG
                     YYFRPTDQTFSVSLFLQDYLPGDKSFRVNLTGVYGSGLPMLDSSVPDQKSFRMPSYKR
                     IDVGFTKSFIDNGKRLVGNLKIANLSVGVDIFNLFNFNNTVSYLWVQTVANQEGNSYR
                     FAVPNYLTSRRVNVRFQMNF"
     gene            complement(8261..8464)
                     /locus_tag="PK049_07070"
     CDS             complement(8261..8464)
                     /locus_tag="PK049_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61356.1"
                     /translation="MNSRRRGFNTEKLKRVHRKEILFNTSELEAINHYCKRYKVRNKS
                     KFLREAIISKILNKFDQDYPRLF"
     gene            8702..11185
                     /locus_tag="PK049_07075"
     CDS             8702..11185
                     /locus_tag="PK049_07075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOK61357.1"
                     /translation="MLQNHNNNLGLGYPFNVRHFLSNLNRYYKAGFWFWDSNTNRIHF
                     GKKFVNALGFKESDNDNIPLDEFFKLVHRDDKKSLEKSFQRLRHNSALHQEMDFRLKI
                     REEWQHKRIFAFAVKDNPDASSYYIIGQFRNVDPVKSDTNTHDESITFERFANLLHKH
                     QLALLEIDLDGTVVNWNKSTTRLFQFDSSNTQSVSLREGFADDTWKKFQEWLLSGSGK
                     PFTSIYRNISGNEFNLSWSKLADIPQDKKRFIVAARDISQLVEFQNQIKRHDFQNEVI
                     SSFFSQIKGQTTPDEIFLLLGQTIEKAFPKSISIVFSYSADDSFVTLESIFGVKTKAW
                     DTFIVELGWNPVGRRLFIEKEKLATLISANVQENIIPFNELLDGIVSATTFKVFERTF
                     KIGKNYISGIVKDDKLYGGIILFQTSDSEPIDTQFLNRISIVASTVIDMSKANLDSCQ
                     MVDGLKKQLAEKYELLTYINHSIRTPLNSIIGFSSMLDFTELDKSLQTEYLRIIQNQS
                     KSLLDLTNELQDFVRISTGALTVIKTKQNVNDFFHELKANIDFRLGLLELYNIKVNYT
                     IPENTDFLELYIDSGRLNQALSIYFIQFIKFIQTGSLNIGYSIEDDKIKIFIHEGDSS
                     IDSKIRTLFTDDLKALLNGTYSHSSHFNILLANRLLTLLEGEVEILNDGKLKLEVKFG
                     LIANETQPQADELSEEQAIKRVEFKNSVVLIVEDEEINYLILNELLTAWGVSTIWAKN
                     GREAVDLVSSLNQGINLILMDIRMPVMDGYAATMEIKQVNSAIPVVAQTAFSAPQERL
                     KAQAAGCNGYITKPIDPNILHHVLEMYLA"
     gene            11240..13021
                     /locus_tag="PK049_07080"
     CDS             11240..13021
                     /locus_tag="PK049_07080"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOK61358.1"
                     /translation="MTLTIQERLSLLRKQMKKADVDAYIVHHSDPHLSEYIPDYWKER
                     EWLSGFTGSAGTLVVTQKNAALWTDSRYFIQAEMQLSGTGIELCKIGMPETPDIQSWL
                     ALNLKQNSIVACNGLLISLSNQRTLSENLKAKKFKVRFDTDLVSKIWDDRPPIPQQKV
                     FVHTAEFAKVSVQEKLGRIRKELLVRNATAYLMCALDEICWTFNIRGADISYNPVFLA
                     YAIVDDDKATLYVDENKLTDEVIQFLANEQVSVKPYSKIFDKLAKLKKKDVLVLDPAK
                     VNFSIYSAIPGNTKVIETTGLATQIKARKQPVEVEGINDAMVQDGIAMVEFLYWLEQN
                     VKSGKVIEITVAEKLLEFRSKRKNFISESFGSIVGYADHGAIVHYSATPQTSYTIKPE
                     GFLLVDSGGQYLNGTTDITRTIHLGNPTPQEKRDFTLVLQGMVRLSMAKFPKGTRGSQ
                     LDTYARMALWSQGLNYGHGTGHGVGYFLNVHEGPQQIRPENHLPIEPGMVMSNEPGLY
                     RPNAYGIRIENLIVCVEDETNDFGSFLRFDTLTLCPIDIKAIDLDMLTVQEREWLNHY
                     HQLVYFKLSPHLAAEHEKWLKEITKPL"
     gene            complement(13061..13477)
                     /locus_tag="PK049_07085"
     CDS             complement(13061..13477)
                     /locus_tag="PK049_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOK61359.1"
                     /translation="MKSYRKELWFETRNRREYINITRDVEVCLAESGIKEGLCLVNAM
                     HITSSVFINDDESGLHHDFEVWLEKLAPEKPYSQYKHNGFEDNADAHLKRTIMGREVV
                     VAVTNGKLDFGPWEQIFYGEFDGKRRKRVLVKIIGE"
     gene            complement(13480..15213)
                     /locus_tag="PK049_07090"
     CDS             complement(13480..15213)
                     /locus_tag="PK049_07090"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOK61360.1"
                     /translation="MVEKEIMEKAKQWLNPPFDEETRARVAQMIEQDPKELTESFYCD
                     LEFGTGGLRGIMGVGTNRMNIYTVGMATQGLCNYLKNNFKHLPEIRAAIAHDSRNNST
                     LFANVAAQILSANGIKVYLFDALRPTPELSFAIRTFKCQCGIVVTASHNPKEYNGYKV
                     YWEDGGQIVPPHDKGIISEVKAISSIEQVNFKGNDNLIVKVGRELDEIYLNTLSTLSL
                     SPDIIEKYRDMPIVYTPIHGTGVNLVPMALRKFGFKNIITVDEQCVVDGNFPTVASPN
                     PEERATMQMAIDKAIKTNAEIAMATDPDADRVGIGIRKPNGEFTLLNGNQAASILIYY
                     ILKKWHEHNRITGNEFVVKTIVTTDLISSIAHGFGVDCYEVLTGFKYIAEKIREFEGV
                     KKFIAGGEESYGYLYGDFVRDKDAVISCAMFAEIAAWAKSQSKTLYDILSDIYREYGL
                     YKEHLISLTRKGIDGLAEIKAMMDAFRSKPPQTIGGSKVTSIIDYQNQVFVDVHTGKE
                     KHTNQPKSDVMQFITADGTKVSVRPSGTEPKIKFYIGVKGTMAANDDIDTALAKLDKK
                     IETIVAELNLR"
     gene            complement(15235..16092)
                     /gene="rfbD"
                     /locus_tag="PK049_07095"
     CDS             complement(15235..16092)
                     /gene="rfbD"
                     /locus_tag="PK049_07095"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOK61361.1"
                     /translation="MKILVTGANGQLGTAIKKESSTFPDLTIFYTDIEETDICSKESV
                     QLALKKYAPNVVVNCAAYTAVDKAEDEEDKAFALNAHAPANLAEQCKLMGVKLIHIST
                     DYVFDGKSNVPYVETDKVNPQSVYGKSKLEGELRSMALGNTMVIRTSWLYSAYGNNFM
                     KTMLKYGKERPELRVVFDQTGTPTLANDLAYAILRIAESPERLFIPEIFHFSNQGVCS
                     WYDFAYEIIKQAGLTTKITPITTSDYPTKAIRPQYSVLNKQKITNLLEMEIPHWRQSL
                     VKCLQEYLR"
     gene            complement(16098..16664)
                     /gene="rfbC"
                     /locus_tag="PK049_07100"
     CDS             complement(16098..16664)
                     /gene="rfbC"
                     /locus_tag="PK049_07100"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007558911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HOK61362.1"
                     /translation="MTIEPTQIPDVLIIKPKVFTDHRGYFFESYNVDAFKKMGIEINF
                     VQDNESYSRKNVIRGLHYQLSPYSQTKLVRVVAGRVFDVAVDIRKNSPTFGQYVGVEL
                     SDENKYQLLIPKGFAHGFAVLSDFAIVSYKCDQYYNPEADRGIRYNDPEIGIDWKIPM
                     EQAIVSAKDNVHPAMKDAELNFVFGSTY"
     gene            complement(16676..17554)
                     /gene="rfbA"
                     /locus_tag="PK049_07105"
     CDS             complement(16676..17554)
                     /gene="rfbA"
                     /locus_tag="PK049_07105"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HOK61363.1"
                     /translation="MKGIILAGGAGTRLYPITKSISKQIIPIYDKPMIYYPLSVLMLA
                     QIKDILIISTPKDIHLYQDLFGDGSQLGLNIQYAIQPSPDGLAQAFIIGENFIGTDSV
                     CLVLGDNIFYGGNLSELLKESAKLNDGAIVFGYYVKDPHRYGVAEFDETGKVLSIEEK
                     PENPKSNFAVTGLYFYSNDVVEKAKSLKPSKRGELEITDLNRLYLNEGRLNLKIMGRG
                     MAWLDTGTHESLLQASNFIQTIEDRQGLKVACLEEIAYRNGFISKEQLLKLAEALANN
                     QYGQYLYRIANEKVYL"
     gene            complement(17631..18569)
                     /locus_tag="PK049_07110"
     CDS             complement(17631..18569)
                     /locus_tag="PK049_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008768242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOK61364.1"
                     /translation="MRKRVLVTGGAGFIGSHLCERLLSEGNEVICLDNYFSGNKQNIV
                     HLLANPYFELVRHDVTVPYYAEVDEIYNLACPASPIYYQQNPIKTIKTSVMGAINMLG
                     LAKRVKAKILQASTSEVYGDPEIHPQPEHYWGNVNPIGPRACYDEGKRCAETLFVNYH
                     MQNGVKIKIARIFNTYGPRMSLSDGRVISNFTVQALTNQNITIYGDGSQTRSFMYIDD
                     LVDALIMLMNTPESVTGPINIGNPEEYTIIDLAKKIVALTGSKSEIIYKAATVDDPKQ
                     RKPDMEVARKILPKWTPKVNIDDGLTKTIEYFKQQL"
     gene            18756..20591
                     /gene="rpsA"
                     /locus_tag="PK049_07115"
     CDS             18756..20591
                     /gene="rpsA"
                     /locus_tag="PK049_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOK61365.1"
                     /translation="MTMNDEMAKEALKNQNAGIPDFDWTSYADDHAYDQNQRKELEKV
                     YDQTLSTVAENEVVEGAVVAITKREVFVNIGYKSEGVISINEFRYNPDLKVGDKVEVY
                     VESSEDKKGQLVLSHKKARVMRSWERVNEAFDKNEIIKGYIKCRTKGGMIVDVFGIEA
                     FLPGSQIDVKPIRDYDVFVGKVMEFKVVKINHEYKNVVVSHKALIEEELEQQKKDIIA
                     KLEKGQVLEGTVKNITSYGVFIDLGGVDGLIHITDLSWGRVNHPEEVVQLDQKLNVVI
                     LDFDDDKKRIALGLKQLTPHPWDSLDPNLKVGDKVKGKVVVIADYGAFVEIAPGVEGL
                     IHVSEMSWSQHLRSAQEFLKVGDEVEAVILTLDREERKMSLGIKQLKPDPWTSIEEKY
                     PVNSKHKAKVRNFTTFGVFVELEEGVDGLIHISDLSWTKKIKHPAEFTNIGDEIEVVV
                     LEIDKENRRLSLGHKQLEENPWDVFETIFTVDSVHEGTIVEIFDKGAVIALPYGVEGF
                     ATPKHLVKEDGSTAKVDEKLAFKVLEFNKSAKRIIVSHSRTFEDEKKVEEKAEKKSKA
                     SSAQKVVKKLNSNIEKTTLGDISELQALKSELEANQKKSDSKSEE"
     gene            20633..20995
                     /locus_tag="PK049_07120"
     CDS             20633..20995
                     /locus_tag="PK049_07120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOK61366.1"
                     /translation="MEFKIQKFEKFTQIEVLEEKLDTNIAPSLKSELVLISGNGERNI
                     VLDLSSCRYCDSSGLSAILVANRLCKNAGGTFVLTGLTDAVERLITISQLDTVLNIAY
                     SKDEAVQIIEKAENEAKK"
     gene            20992..21921
                     /locus_tag="PK049_07125"
     CDS             20992..21921
                     /locus_tag="PK049_07125"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HOK61367.1"
                     /translation="MTFSVTILGSSSALPTSKRFPSAHVLNVHERLFLIDCGEGTQIQ
                     LRRFKLPMNRINHIFLTHSHGDHVLGIFGLISTFNLLGRKYDLHVYGVADFEEIFYKN
                     IDYFTDQMSFKVIYHVVDPFKVETIYSDKHVVVTTLPLRHRVPTVGYLFREAPRPLNI
                     RKELIAKYNLSLTDIVKIKNGSDYTLNDGTVIPNAELTYISSLPRSYAYCSDTLYSER
                     LVGLVKNVDLLYHEATFLHSDLRLARLTGHSTAQQAATVALKASVGKLLIGHFSSRYK
                     NEKDLQREAMEIFPATEVVNDGLTFSVEPKSTY"
BASE COUNT         6261 a         4393 c         4701 g         6572 t
ORIGIN      
        1 attgatgagg ttggccgaat ggaactttat ggtgaagttt gggcagagct gatagaacat
       61 catcttacta aaactaagaa tgttttagta ttaacggtaa ggcgcgaaaa cctaatgcat
      121 gtagtggagc ggtggaacct aaccgatgcc tacgtatttg atattaataa ggttggtgta
      181 accgatgctg caaatagcat taaaaattta gttctcagtt accacgcggc cagttcccga
      241 aagtaatgaa cgtgaatcaa taattgatgt accgtggatg gcagggtaaa caattactac
      301 atttttatcg gagattattt ggctagtgaa tatcagagtg ttttggatat cgctattgta
      361 agcaatagtt cctttgcgtg ccgatattat aattcctaga gcgttactat caatactttt
      421 caggtggtta aaaccttcct cccattttcg tatttccttt acggttggtt ttagcttgct
      481 gaatgtagga ggaagttctg ctataacatt ctcggtatga attttatcaa caatgatggt
      541 aacctgttga cgtgtttttt taataatcct ctcaaggctt ttcatgtagt tcttaaaatc
      601 ggggtcaaac tcagcattcc tagggattaa aatatgcacc tgcgaaattt gagcaattga
      661 ttgcttaagg ctgcaaatgt aaacgggttg ttttacatcg gctaaaaccc cttcgagtat
      721 tgtcccaaaa atccatcggc gcgatgaaac gtatttcccc gaccatccca taataatttt
      781 agaagcaagc atttcctttg ctgctctcac aattcctgat gagatgttga catcaacccg
      841 aacagcccac tccgtatcaa cgccaaaact atggctaatt tcttctgcct tagcagcaag
      901 gtcgttaact ttggagattt cagtctcgtc cttcacgacc ctaagtaagg tgatgggatt
      961 tttcttagtg cgttcactta catatcctgc aaactcaatt aggtgctcaa tgtttgaggg
     1021 gtttgcaact ggaatcaaca ctttttcagt atcggagggt ttaaacttta cagcagggaa
     1081 ggctacggca taactccttg cagccctttc ggttaccatt gcactcacaa cacacgaggc
     1141 aaaaatcacc acaatagtgg cgttcaacac attaatatcc acaataccaa tcttgtaacc
     1201 aaccagaata attgctatgg tagcggccgc gtgagcggtg ctcaaaccaa aaattagttg
     1261 ccgatcgagt gtttttagct taaagatttt ttgagtgagg tatgccgcca accattttga
     1321 gattatggcg aatgatatga gaattgcaga tagttcaagc gattcgtatc cgcttacaaa
     1381 agcttttagg tcaattagca ttccaacgta aaaaagaaag atagggataa atagggcgtt
     1441 tccaataaac tcgatacgat tcatgagcat ggctgagggt ggaatttgcc tgttaagtac
     1501 tattcctgcc aggaaagccc ctattatggg ttcaattcct aaataccgtg caattgcact
     1561 gttagtaaat actgttgcaa gaacaaaaac gtattgagcc atttggtcgg aggaaaggtt
     1621 tcttagaaac cagtgggtaa atcgaggaag taatagaatg ataaaaagga caaatgccag
     1681 tatcttgggt ataagcagaa ggaatccatt taaaccgcta tttgctgata acgatgttat
     1741 gataacaaaa atgagaagaa caagggtatc ggttattatt gttccaccaa ttgatatttc
     1801 aaccggcaat gtttttgtga gtcgaaggcg cgaaactatt ggaaatgcaa tcatggtttg
     1861 agttgagaac atgcttgcaa tcaggagcga tggcaaataa ccaaacccca gaaaatactt
     1921 gcagatagca aaaccaaata gaaaaggaat tgtaaaggtc aaaagaccaa aagtgatgtt
     1981 cttgcgttga ttttttaaaa aacttccaac ctctaattct agtccggcca aaaacattaa
     2041 atatattagt cctacgtttc caagtagttc aatacttctc tccttatcaa gaatgtttag
     2101 tccagatgtt cccagtatca tgcctgcaat aataagacca atgatgccag gaagttttag
     2161 ctttgaaaaa actaaaggga caataaaaat caccaaaagg gtaattccaa atactgtaat
     2221 gggttcacta attgggagat gaaaccctaa attttccatt tgaagttgga ttaaattttg
     2281 cgtaaaatac taaaggttaa cttaaaaaaa aagccacctg gagggtggct tttaaatcaa
     2341 tcactcaaat tatttgtttt tttcgctgaa ctcacgccct aaccggagtg ccttaaggtt
     2401 tagctgaacc acctcgtcgc cttttctacc aaaaagctgg cgtattcctt cttcgatttt
     2461 ttcgaaagga atatcgagga atagcgaggc agcgccaagc attactatgt ttgaagcctt
     2521 tgttgagcct gcttccttag caatggcatc ggcatcaata gcaatgtggt tctttacagc
     2581 ttttattttg gatagtatat cgttgatatt ggggtaatcg ggaatgttgt taaagggggt
     2641 aacattggtt accaaccagc ctgtttgagc caaaaagggt aaatacctaa ggccctccat
     2701 gggctcaaca gaaataatca tatcggcttt gccagccgga atgaggtcgg aggcaattgg
     2761 cttatcggat attcgaaggt gcgactgcac gtcgccgcca cgttggctca taccgtgcac
     2821 ctcggattgt ttgatgtaga gattgctgtt tacggcggca agtccaatgg ctgctgcaat
     2881 ggaaagtatt ccctgtccgc ctacgcctgc aagtattatg tcgtttttca tgattgaagt
     2941 cgttttatgt tactcattag tctctttctt ctcagccttt ttgcgtcggg caaaggtctg
     3001 gatacattca cgctgaggaa taataaccga aacgccattg taagccagct cttccttaat
     3061 gattttcacg ttctcttcgt gatttttctt taagggggtt acaaccctta tgtggtcagg
     3121 gtgaacgcca attcctttac atatttcaac tattcgtccg gtagccgaag aatgctggcc
     3181 tcctgtcatt ccggttgttg agttgtcaga aataataact gtgatgggcg agttctctac
     3241 aacagcgtcc aaaaggccgg tcattccgct atgtgtaaag gttgaatcgc caataacagc
     3301 aacagcagga acaagacctg catcggcagc acctttagcc atggtaatgg aggcacccat
     3361 atcaacacag gagttgatag cattgaatgg aggcagcgca cccagggtgt agcaaccaat
     3421 atcggagaaa acccttcccg ttccgtattc ctttagtgcc tcattcaaat cctcgtaaac
     3481 atccctatgc ccacaaccct gacagagtga tgggggacgg ttttttacaa cctcgggcac
     3541 aggctccccc tcagtaaccg ttagcccaag ggctttggca acaatgttgg ggttaagttc
     3601 gccatcgcgg ggtaatgttc catcaagtcg tccgtgaatg gttttgccaa tgtttaggaa
     3661 accttttatc atttcctcaa caagggggta tccttcctca agtatcaaaa gcttactaca
     3721 gctgttatag agttcagtaa cctgtttgcg tggtattggg tactggcaaa ttttaagaac
     3781 aggatatgga catttatggt cagggaaatt ctcgataagg taattgtaag ctatgccagt
     3841 tgttattata cctaaacttt tatcggttcc ctcaatcatc tggttatagg ccgatttttc
     3901 agactcaagt tccattgcag tttgggaaac cagcagtttt ttgtagttct tgcgagcaat
     3961 ggagggaagc aaaacaaact ggcgtggatc cgatggcagt cggatttcat tttggggtct
     4021 tgactcgcga cgctgcaccc cggctcttga gtgagccatg cgtgtagtaa tacgcattaa
     4081 tacaggaacg ttaagccttt cggatagttc aaatccgaaa tatgccatgt cgtaagcctc
     4141 ctgttggttc gatggttcca gaatgggaat cattgagaac ttaccgtaaa agcgtgaatc
     4201 ctgttcattc tgtgaggagt gcatcgatgg gtcatcggct gaaaccacaa ttagcccacc
     4261 attaaccccg gtaatggagg cattaataaa agcatcggcg gctacgttaa gcccaacatg
     4321 tttaaaacat accatggccc ttttaccggc atacgacata ccgatggcgg cctccatggc
     4381 agttttctca ttagctgacc attgcctgtg aatattcaat tcggctgctt gctttgaggc
     4441 ttgcacgtat tccattattt cggttgatgg agtgccagga taagcgtata tccctgaaag
     4501 gcctccatca attgcgcctt gcgctattgc ttcgtcgcct aacagtagta acttgttgtt
     4561 attcatattg ttattatgtt tcttgttgtt ttacttgggt tgaattttga gtagtaaagc
     4621 cttaagtgat aaataataaa cttatagacc gccaaaacgc tttaaaaagc caacattcaa
     4681 tgctacatta tgcaggtagt aaataaaact tacatccatt aaacttacaa tttgtaattg
     4741 atgctggtta atcaatttaa tgttgttagc aaaaatacaa aaaacaataa tcgcaataaa
     4801 gtaaaatgct gaaaaatata aaatattgta attgagtggt tattcaataa cagttgcaat
     4861 aatttttcga ctcccgccgt gttttcggaa ctcacaaagg tatattcctt gccaagttcc
     4921 caggtttaat cttccgttag ttatgggtat agatatcgat gttcctacaa ctgtggtttt
     4981 tatgtgtgct ggcatatcgt catcgccttc caggtcgtga ttaaagtatg gctccatggg
     5041 gtagtgcctg ttaaagaaag cattcaggtc agttctaacg ctggggtctg cattctcatt
     5101 gatgctcagt gccgctgatg tatgcttaat gaataggtga agtaaaccta ccagtggtag
     5161 ttcaggtagc tggtttacta tttctgcagt taccagatga aaaccttggg ggtaaggtct
     5221 aagcactatt tctatctgtt ttatcatact acatcaacct tttaatgtgt tggtgaatga
     5281 aatcgaaagc ccagttctca atgtcatctc tcagagtatc gtaggcttta cgggtaagcg
     5341 aatcgtcact cttagcagct ttagggctgt cgaaatgaaa tacatatagg tttttatatt
     5401 gcagattact gcttgcagtt tttgccacct tattatccaa aaccaaaaca atgtcgaatt
     5461 ccttgtcggt aagcgagtta aggcttttgg gttgatgctt ggttatatca attacagcat
     5521 ccatcatgct ttgagctgcg ttaaggtcaa gttgtgtagg ttcaattcct gcgctggtta
     5581 catttgctgc accacgagca tagtatttaa accaaccttc ggccatggcc gaacggcagg
     5641 cgttgttctg cgaaactata agtatttcct tcatcctttt tcagttatga ttttccgaga
     5701 ataacaaaaa acacaataaa ggtaaagatt ttgagtttat ggtttacgat gtttggttcg
     5761 attactacct ttggggttaa atttccaatg gctaaatgag cgtaaaaccg ccatattaca
     5821 tctttcaatg tgttttggtg atatttatca ccggttttgc gtcgctacat gctcaggagc
     5881 gaagggtgaa gatatctggc attgttaagg atacgcttaa tatgcctata ccctcggcaa
     5941 gcgttattat tgtaggaagt tctttaggtg tttcaaccga cgatgagggg cgcttctcaa
     6001 tcacagtaac cgatattgat acaatatcta ttaggttttc gtgcctggga taccaaacac
     6061 aggaaatgaa tgttaaagct gaaaggggta agttccttga ggtaatttta aaaccatcgg
     6121 aagagcatat tgaaggggtt tacgttcgtt cgcagcaacg gaaggtggga aacattgagc
     6181 gcattggaat tgatggatta aaaacgtccc cgaatgtgtc gggcgggttg gagtcactgg
     6241 taaagctttt gcccggagtt tcatcgtcca atgagctgag ctcacagtat agtgtaagag
     6301 gagggaattt tgatgaaaat ttggtttacg ttaacgatgt tgaggtttac aggcctttcc
     6361 tgattcgctc agctcagcag gagggtttaa gtttcataaa ccccgatatg gttggggcgg
     6421 ttgagttctc atcgggtggg tttacggctg aatttggcga taagatgtcg tcggtgctta
     6481 atgtgcgtta ccgtgagcca aaggcgaatg cgttaagggt cagtgccggt ttgctgggtg
     6541 cttcggcaac cctggaagga gttgccattg gcagctcgct tacctacatg tcaggggtgc
     6601 gctataagac atcgcgttat atactcaact cactggaaac caaaggcgat tatactcctt
     6661 cgttcttcga ttggcaaggc atagctgact ataaactctc cgagaggatt accattgggc
     6721 tgttgggcaa tgtggccata aaccgtttcg actttcagcc aagggtacgt gaaacccgtt
     6781 ttggtgtttt taacaacacc cttcagctaa aaatatacta cgatggccaa gagaaagaca
     6841 ggtatttatc gtcaatggct gccttaatta ctacaattag ggtaactccc aattttaatg
     6901 tcaaggtaat tgggtcatca tattactcaa acgaacggga aacatacgat ttgcttggac
     6961 agtatttcct gaatgagctc gataactcca tgggatcaaa aacttatggc gatagcctga
     7021 taaatgttgg gattggagga tttctggatc atgcccgtaa ccatatcaac cttaaagttc
     7081 aaagcttaaa cctgcttggc gatatgtggt tcgggaaaag cctaattaaa ttgggggtgc
     7141 agttgcagcg cgaagtttta agtgatgagc tcctggagtg gggattggtt gattcatcgg
     7201 gatacgctat accctacaca ggctcacagg ttgggcttga tagatttttt ggggcagcct
     7261 caacaattga gtcgtaccgc gcattagggt ttgttcaggc cgatttacat tttaacgttg
     7321 gaaattggca gtatagaact aatataggat tcagggtaag ccactggagt tttaacaagg
     7381 aaattctttt tagcccccgc ttagcactca gctttaatca ttcaaactat agaagtttac
     7441 agtatcacct ttcgtttggg tattactatc aacctccctt ttatagggag attcgtttgg
     7501 ctagcggtga gctaaatagg aacatcaaag cgcaacaatc cattcatgca cttgcaggag
     7561 ccgagtatta ctttacagct tggagccgac cattccgaat gagcctagag ttctataata
     7621 agtggctaaa ccgtttaatt ccatactcag ttgataacgt tcgtttacgc tactccggcg
     7681 aaaacctggc aaggggatac gcacgaggag ccgacctaaa gataaatggt gagctggttt
     7741 ccggagccga atcgtggctc agcctttcga ttctcaaaac aaaggaggat aatctttacg
     7801 actcatatac cgatgaaggt gggaatacgg tttaccccgg atactatttc cgccctaccg
     7861 atcaaacctt ttccgttagt ttatttttgc aggattacct gcctggcgat aaaagcttta
     7921 gggttaacct taccggggta tacggcagtg gcctgccaat gttggatagc tccgttcctg
     7981 atcaaaaaag ctttaggatg ccatcgtaca agaggattga tgttggattc acaaaatcct
     8041 ttattgacaa tgggaaacga ctggtgggta atttaaaaat tgctaacctc tcagtagggg
     8101 tcgatatttt taatcttttc aactttaaca atacggtttc ctatttatgg gtgcagactg
     8161 tagccaacca ggagggtaac tcctatcggt ttgccgtgcc caactatcta acttcgcgca
     8221 gagttaatgt taggtttcaa atgaattttt agcaatacct ctagaataaa cgggggtaat
     8281 cctgatcgaa tttgtttaaa atttttgaga tgattgcctc acgcaggaac tttgacttgt
     8341 tccttacctt ataccgcttg cagtagtggt taatggcttc taactcgctg gtgttaaaca
     8401 gtatctcttt tctatgaacc cgttttagct tttcggtatt gaaccctcta cgtcggctat
     8461 tcatcggtta aatgactttt ttgcaaataa accaactttt ttcatcggtt ttcattattg
     8521 gttcatacag ttatcaaaaa gtttttaaca actgctatat gtaaacctct ttaatacttt
     8581 agcttgtagc aaattagttc ctttttgcca tttaacttac actgatctat tttgattatt
     8641 acccaaaacg ttttaaattt aggatttatt aacataacct tagtcattat aaccatacgt
     8701 tatgctgcag aaccataaca ataaccttgg cttaggctat ccttttaacg ttaggcattt
     8761 tctgtcgaac ctaaaccgat actataaagc aggattttgg ttttgggatt caaacaccaa
     8821 tagaattcat tttgggaaaa agtttgttaa tgctttgggc tttaaggaat ccgataatga
     8881 taacattccc cttgatgaat ttttcaaatt agttcataga gatgataaaa agtccctgga
     8941 aaaatcgttc cagaggttaa gacataattc tgccttacac caggagatgg attttagatt
     9001 aaagattcgt gaggagtggc agcataagag gatttttgca tttgcagtta aggataaccc
     9061 cgatgcaagc agttactaca ttattggaca gttcagaaat gttgacccgg tcaagagtga
     9121 tacaaatacc catgatgagt ccattacatt cgaacgtttt gccaacctac tacataagca
     9181 tcagcttgct ttgcttgaga ttgatttgga tggcaccgtt gtgaactgga acaaaagtac
     9241 cacaaggctt tttcagttcg attcatcgaa tacacaatca gtttcgctta gggaaggctt
     9301 tgccgatgat acctggaaaa aatttcagga gtggttgctt agtggttcag ggaagccttt
     9361 tacctcaatc tatagaaata tcagtggaaa cgagtttaat ctaagctggt caaaacttgc
     9421 cgatattcct caagataaaa aaagatttat agtagcagca cgcgatatta gtcagcttgt
     9481 tgagtttcaa aaccaaatca aaagacatga ttttcagaat gaggtaatat catcgttctt
     9541 ctctcagatt aaagggcaaa caacacccga tgagattttc ctactgcttg gccaaaccat
     9601 tgaaaaggct ttccctaaat caatatccat agttttttcc tacagtgccg acgatagctt
     9661 tgttacgctg gaatcaattt ttggggtaaa gaccaaagcg tgggatactt ttattgttga
     9721 gctgggttgg aacccggttg gccgtaggct ttttattgaa aaggagaaac ttgccacctt
     9781 aatttcggcc aacgtacagg agaacattat tcccttcaat gagttgcttg atggcatagt
     9841 atcggcaaca acctttaaag tatttgagcg aacctttaag ataggcaaga actacatttc
     9901 cgggattgtt aaggacgaca aactgtatgg tggaatcata ctatttcaaa catccgattc
     9961 tgaaccgatt gatacacaat tcttgaatag aatatcgata gtagcctcaa cggttatcga
    10021 tatgagtaaa gcgaatctgg attcatgcca aatggttgat gggttaaaaa aacagcttgc
    10081 cgagaagtat gagctattaa catacattaa tcacagcatt agaaccccgc ttaactctat
    10141 aattgggttt agcagtatgc tcgattttac tgagttagat aagtcgctgc aaaccgaata
    10201 ccttagaatt attcaaaacc aaagcaaatc gctgttggac ctcaccaatg aattacagga
    10261 ttttgtcaga attagtactg gggcgctcac agttattaaa accaagcaaa atgtaaatga
    10321 cttttttcat gagctaaaag caaatatcga ttttcgattg gggttgctgg aactctacaa
    10381 cataaaggtt aactatacaa ttcctgaaaa tactgatttt cttgaactct acattgattc
    10441 cgggcgttta aatcaggcac taagcatcta tttcattcaa ttcataaaat tcattcaaac
    10501 cggaagccta aatattggat attcaattga ggatgataag attaagattt ttattcatga
    10561 aggggattca tcaattgact caaaaatacg aacattattt actgatgact taaaagcctt
    10621 gctaaacggc acctatagcc attcttccca tttcaacata ttgttggcca acaggctttt
    10681 aaccttactt gagggtgagg tggagatact caacgacggc aagttaaaac ttgaggtgaa
    10741 atttggttta attgctaacg agacccagcc ccaagccgat gaactaagcg aggaacaggc
    10801 cataaagaga gttgaattca agaatagtgt agttctaatt gttgaagatg aagagattaa
    10861 ctatttaatc ctaaacgaac ttctgacggc atggggggtg tcaaccattt gggctaaaaa
    10921 tggtagagaa gcagtggatt tagtcagttc gcttaatcaa ggtattaacc tcatccttat
    10981 ggatattagg atgccagtaa tggatggtta tgcagcaact atggaaataa aacaagttaa
    11041 ttctgctatt cctgttgtag cccaaacagc cttctcggcg ccacaggagc gtttaaaagc
    11101 tcaggcagct ggatgtaatg gttacatcac aaaacccatc gaccccaaca ttttacacca
    11161 tgttcttgaa atgtatttag cctgaatcat tgcaataagc tatttttgta acccattccg
    11221 agtttaacat taaaaagata tgactttaac cattcaggag agattaagtc tactccgaaa
    11281 gcaaatgaaa aaggctgatg tagatgccta cattgtccac catagcgatc cacatcttag
    11341 tgagtatatt ccagactact ggaaggaacg cgaatggctg tcggggttta caggttctgc
    11401 tggcacctta gtggttaccc agaaaaacgc tgccctgtgg actgactcaa ggtattttat
    11461 ccaagccgaa atgcagctaa gtggtactgg catagagctg tgtaaaattg gtatgcccga
    11521 aacgcctgac attcaatcgt ggttggctct taaccttaag caaaactcaa tagttgcttg
    11581 caatggactg cttatctcac tctcgaatca aagaacccta agcgagaact taaaagcaaa
    11641 aaagttcaag gtaaggttcg ataccgattt agtgagtaaa atttgggacg atagacctcc
    11701 catacctcag caaaaagttt ttgttcatac tgctgaattc gccaaggttt ccgtacagga
    11761 gaaactggga agaataagga aagagctact ggtacgaaat gctacagcat acctgatgtg
    11821 cgcactcgat gagatatgct ggacttttaa tattaggggt gccgatattt cgtacaatcc
    11881 tgtttttctt gcatatgcaa ttgttgacga tgataaagca acgctatatg ttgatgaaaa
    11941 caagctaacc gatgaggtta tacaattcct tgccaatgag caggtatcgg taaaacctta
    12001 cagtaaaata ttcgacaagt tagcaaagct taaaaagaag gatgtactgg tattagaccc
    12061 tgccaaggtt aacttctcaa tttattcagc aattccaggt aacactaaag ttattgaaac
    12121 aactggtttg gcaacccaaa ttaaggcccg aaaacaacct gttgaggttg agggtattaa
    12181 cgatgcaatg gtgcaggatg gaattgccat ggttgagttc ctatactggt tggagcagaa
    12241 tgttaaatcg ggtaaggtca ttgagattac tgttgccgaa aagttactgg aatttaggag
    12301 caaacgcaag aattttatca gtgagagttt tggaagtatt gtaggatacg ccgatcatgg
    12361 ggctatagtg cattatagcg caacccctca aactagctac accatcaaac ccgaaggttt
    12421 cctattggtt gattccggtg gacaatacct aaatggcact accgatatta cccgcactat
    12481 tcaccttggt aacccaaccc ctcaggagaa gcgtgatttc acgcttgttt tgcagggtat
    12541 ggtaaggctt tctatggcaa agttccctaa gggtacgcgt ggttcacaac tcgatactta
    12601 tgcccgaatg gctttatgga gtcaggggct aaactatggg cacggaacag ggcatggtgt
    12661 tggatacttt ttgaatgtgc atgaggggcc gcaacagatt agaccggaaa atcatcttcc
    12721 cattgagccc ggtatggtta tgtccaatga acccggatta taccgaccca atgcctatgg
    12781 cattagaatt gaaaacctga ttgtttgcgt tgaggatgaa acaaacgact ttggttcatt
    12841 cctacgtttc gataccctta ccctttgccc aattgatatc aaagccattg accttgatat
    12901 gctaaccgtt caggagcgag agtggttaaa ccattaccat cagttggttt actttaagct
    12961 gtcgccacac ctggcagctg agcatgaaaa atggctaaag gaaattacaa aacccttata
    13021 ggaaaataaa gaaggctgtc atttactgac agccttttaa ctattcacca ataatcttaa
    13081 ccagaaccct ttttcggcgt ttgccatcga attcaccgta gaaaatttgt tcccagggac
    13141 caaaatcgag tttgccattg gtaacagcaa caaccacttc gcgtcccata atggttcgtt
    13201 ttaggtgtgc atcggcatta tcttcaaacc cgttgtgttt gtactgtgag taaggttttt
    13261 cgggtgcaag cttttcgagc catacttcaa aatcgtggtg caatccactt tcgtcgtcgt
    13321 tgatgaacac gctcgatgta atgtgcatgg cgtttaccag gcatagtcct tccttaattc
    13381 cgctctcggc taggcaaact tcaacatcgc gtgtaatatt gatatactca cgccgattcc
    13441 gtgtctcaaa ccatagctct ttgcgatacg atttcattgc tatctaaggt taagctctgc
    13501 tacaatagtt tcaatttttt tgtcgagttt tgccagcgcc gtgtcaatat catcgtttgc
    13561 agccatagtg cctttaacac ctatgtagaa ttttattttg ggttcagtcc ccgatgggcg
    13621 aaccgatact ttggtgccat cggcagtgat gaactgcatt acatcggatt tgggctggtt
    13681 tgtatgcttt tcctttccgg tatgaacatc tacaaaaact tgattttggt agtcgataat
    13741 ggaagttacc tttgaaccgc caatggtttg tgggggttta cttcgaaaag catccatcat
    13801 cgctttaatc tctgctaagc catctattcc cttacgagta agtgagatca aatgctcttt
    13861 gtacagtcca tattccctgt aaatatcgct cagaatatcg tacagggttt tgctctggct
    13921 tttagcccaa gctgcaatct cggcgaacat ggcgcagctg attaccgcat ctttatcgcg
    13981 gacaaaatcg ccatacaggt aaccgtagct ctcctcgcca ccggcgatga atttctttac
    14041 cccttcaaat tcgcgaatct tttcggctat atatttaaat ccggtgagca cctcgtagca
    14101 atcaacccca aatccatgag cgatggaact aattaggtca gtggttacaa ttgttttaac
    14161 cacaaactca ttaccggtaa tgcggttatg ttcatgccac tttttgagaa tatagtagat
    14221 aagaattgaa gccgcttggt tgccgttaag cagggtaaac tcgccgtttg gctttcgaat
    14281 cccaattcca acacggtcgg catcggggtc ggtagccatg gcaatttcag catttgtttt
    14341 aattgctttg tcaattgcca tttgcatagt ggcacgttcc tcggggtttg gcgatgctac
    14401 tgttggaaaa ttaccatcaa ccacgcattg ctcatcaacg gtgataatat ttttaaagcc
    14461 aaatttgcga agtgccatgg gtacaaggtt tacacctgtt ccgtgaattg gggtgtaaac
    14521 aataggcata tcgcgatact tttcaatgat atcgggcgaa agtgaaagtg ttgaaagcgt
    14581 attcaggtaa atctcatcta actccctacc aactttgaca attaggttat cgttcccttt
    14641 aaagttaacc tgctcaatgc ttgaaatagc cttaacttca ctaattattc ccttgtcgtg
    14701 gggtggaaca atttgaccac catcttccca gtaaacctta tagccgttgt attcctttgg
    14761 attgtgcgat gcagttacaa caataccaca ctgacacttg aacgttctaa ttgcgaagct
    14821 aagttctggt gttggtctaa gggcgtcgaa caggtaaacc tttatgccat tggcacttag
    14881 aatctgtgct gccacattgg cgaatagggt gctgttgttc cgggagtcgt gtgcaatggc
    14941 ggcccgtatt tcaggtagat gtttaaagtt gtttttcagg tagttacata agccctgggt
    15001 ggccatgcca acagtatata tgttcattct atttgtgcca acacccataa ttccacgtaa
    15061 accgcctgta ccaaactcca aatcgcagta aaacgattcg gttagctcct tggggtcttg
    15121 ctcaatcatc tgggcaaccc ttgccctggt ttcctcatca aaaggtggat taagccattg
    15181 cttggctttt tccattattt ccttttcaac catattgtag tattttttag tgttttacct
    15241 taagtattcc tgtaagcatt tcactaagct ttgcctccag tgtgggattt ccatttccag
    15301 caagttagta attttttgct tattcaacac gctatactga ggtcgaattg ctttagtggg
    15361 gtagtcactg gttgtaatgg gggttatttt tgtagtaagc ccagcctgtt tgatgatttc
    15421 gtaagcaaaa tcgtaccagc tgcaaactcc ctggttggaa aaatggaaga tttccggtat
    15481 gaataacctc tctggtgact cagcgattcg aagaattgca tatgcaagat cgttggcaag
    15541 ggtaggggtt cctgtttggt caaaaaccac tctaagctca gggcgttcct ttccgtactt
    15601 tagcatggtt ttcatgaagt tgttaccgta agccgagtat aaccacgatg ttctgattac
    15661 cattgtattg ccaagtgcca tagaccttag ttccccctca agcttacttt ttccgtaaac
    15721 cgattgtggg tttaccttat cggtttcaac gtaggggaca ttggacttac catcaaaaac
    15781 atagtcggtt gaaatatgta tcagcttaac acccattaat ttgcattgtt cggcgaggtt
    15841 tgcgggagca tgggcattca gggcaaatgc cttgtcttct tcatcttcag ccttgtccac
    15901 tgccgtatag gctgcacagt tcacaacaac attaggtgca tactttttaa gtgccagctg
    15961 aaccgattcc ttgctacaga tatcggtttc ttctatatcg gtataaaaaa tagttaaatc
    16021 tggaaaagta cttgattcct ttttaatggc agttcctagc tgaccgtttg ctccggttac
    16081 aagaattttc atggtagtta gtaggttgaa ccaaaaacaa agtttagctc ggcatccttc
    16141 attgccgggt gaacattatc cttagccgat acaatggcct gctccatagg aattttccaa
    16201 tcaatcccaa tttcaggatc gttgtaccga atacccctat cagcctcagg gttatagtat
    16261 tgatcgcact tgtagctaac tatagcaaaa tcgcttagaa cggcaaagcc gtgtgcaaaa
    16321 ccctttggaa tcaacagttg atatttattt tcatcggaga gttcaacacc aacatattgc
    16381 ccaaatgtgg gggaattctt tctgatatca accgccacat caaatactct accggcaaca
    16441 accctaacca gtttggtttg tgagtaaggc gaaagctgat aatgcagacc acggattaca
    16501 ttttttcttg agtacgattc gttatcctga acaaaattga tttcaattcc catttttttg
    16561 aaggcatcta cattgtaact ttcgaaaaaa taacccctgt ggtcagtaaa aaccttaggt
    16621 ttaataatca gcacatcggg aatttgggtt ggctcaatag tcatactgaa atattttaaa
    16681 ggtaaacttt ttcgtttgca attctgtaaa ggtattgccc gtactggttg tttgccaggg
    16741 cttcggcaag ttttagcagt tgttcctttg agataaatcc atttcggtaa gcaatttctt
    16801 cgaggcaagc aaccttaaga ccctgtctgt cctcaatggt ttgaatgaag tttgatgcct
    16861 gaagcaagct ttcatgggtt ccggtgtcga gccaggccat acctcgtccc attattttaa
    16921 ggtttagcct accctcattt aggtaaagtc tgttcagatc ggtaatttct agctcacccc
    16981 ttttggaagg tttaagcgat tttgcctttt ctaccacatc gttgctgtag aagtaaagcc
    17041 ccgtaacagc aaagttcgat ttgggatttt cgggcttttc ctctatgctt aatactttcc
    17101 ctgtttcatc aaactcagca acaccatacc gatgcggatc tttaacgtag tagccaaaaa
    17161 ctatagcgcc atcgttcagc ttggcgcttt ccttaagcag ctcgctaaga tttccgccgt
    17221 aaaaaatatt atcgcccaat acaaggcaaa cgctatcggt accaataaag ttttcaccaa
    17281 ttataaaggc ttgagccaaa ccatcgggtg atggttgaat ggcatactga atatttaaac
    17341 ccaattgcga accatcgcca aaaagatctt gatacaggtg tatatctttt ggtgttgaga
    17401 taatgagtat atccttaatc tgtgccagca ttagcaccga taaggggtag taaatcattg
    17461 gtttatcgta aatgggaatg atttgctttg aaatgctttt ggttattggg tataaccgtg
    17521 ttccggctcc tcctgctaaa attattcctt tcatggtgtt ttttttaact aatcagtttt
    17581 taaaaaatta cacaaaaata ggctttggca tcagcttcac aaaattataa ttacagctgt
    17641 tgtttaaaat attcaatggt ttttgttaat ccatcatcaa tattcacctt tggtgtccat
    17701 tttggaagga tttttcgggc aacttccata tcgggctttc gctgctttgg gtcgtcaaca
    17761 gtggcagctt tatagattat ttccgatttg cttcctgtta atgcaactat ctttttagcc
    17821 aggtcaatta ttgtatattc ctctgggtta cctatgttta ttgggccggt aacactttca
    17881 ggggtgttca tcaacataat cagagcatca accaaatcat caatgtacat aaagctacgg
    17941 gtttgagagc catcgccgta aatggttatg ttttggttgg ttagggcctg aacggtaaaa
    18001 ttggatataa ctctgccgtc ggataaactc atccttgggc cgtaggtgtt gaaaatacga
    18061 gcaatcttga ttttaacgcc attttgcata tgataattta caaacagggt ttcggcgcag
    18121 cgtttgccct catcgtaaca ggcccttggc ccaatggggt ttacattacc ccagtaatgc
    18181 tcaggttgag gatgaatctc aggatcgccg taaacctcgc tggttgatgc ctgtaatatt
    18241 ttggctttta ccctttttgc cagtcccagc atgttaattg cacccattac cgatgtctta
    18301 atggttttaa tggggttttg ctggtagtat attggcgagg cagggcaggc taggttgtaa
    18361 atttcatcaa cctcggcata gtagggaacg gtaacatcgt gcctaaccag ctcaaagtag
    18421 gggttagcca gcaaatgcac aatattttgc ttgtttccgc tgaagtagtt gtcgaggcaa
    18481 attacttcgt taccctcgga aagcaaccgt tcacaaaggt gggaaccaat aaagcctgct
    18541 ccccctgtaa ccaatactcg ttttcgcatg ttcaatagca tttactttac caaaagtagg
    18601 taatttcggc catatttgat acaaatcaac caaacttgtt gatatacatt tcaaaataat
    18661 tgggtaaatt tgattttatt ttagaaagtt atgtattttt gcaagttgaa ttttatctac
    18721 aaataatgtc taacaaatta acaatctgaa agttaatgac aatgaatgac gaaatggcca
    18781 aagaggcttt aaagaatcaa aatgctggaa tacctgattt tgattggaca tcttacgcag
    18841 atgatcatgc ctacgatcaa aaccagcgca aagaacttga aaaagtttac gatcaaaccc
    18901 tatcaaccgt agccgagaat gaggtggttg aaggtgctgt agttgctatt acaaaacgtg
    18961 aagtttttgt aaacatcggc tacaaatccg aaggcgtaat aagcatcaac gaattccgtt
    19021 acaaccccga tttgaaggtt ggcgacaagg ttgaagttta cgtagaaagc tccgaggata
    19081 aaaaaggtca gcttgtgctt tcgcacaaaa aagctcgtgt aatgcgttca tgggaacgtg
    19141 ttaatgaggc ctttgacaaa aatgaaatta ttaagggtta catcaagtgc cgtactaagg
    19201 gcggtatgat tgtggatgta ttcggtattg aggcattcct tcccggttca caaattgatg
    19261 ttaaacctat ccgcgactac gacgtatttg taggaaaggt aatggagttc aaggttgtaa
    19321 aaatcaacca cgagtacaag aacgttgttg tatcgcataa agcgcttatc gaggaagaac
    19381 tcgaacagca aaagaaagat attattgcta agctcgaaaa gggtcaggtt cttgaaggaa
    19441 ccgttaaaaa tattaccagt tatggtgtat tcatcgacct tggcggcgta gacggactta
    19501 ttcacatcac tgacctttcc tggggccgtg ttaaccaccc cgaagaggtt gtgcagcttg
    19561 accagaagct taacgtggta attctcgatt ttgacgacga taagaaacgt attgcacttg
    19621 gtctcaagca gctcacccct catccttggg attcgctcga tccaaacctt aaggttggcg
    19681 acaaggttaa aggtaaggtt gttgttattg ccgactatgg tgcattcgtt gagattgccc
    19741 ctggcgttga gggcctaatt cacgtttctg aaatgtcgtg gagtcagcac ctacgcagtg
    19801 ctcaggagtt cctgaaagta ggcgatgagg ttgaagctgt tatcctaact cttgatcgtg
    19861 aagagcgcaa gatgtcgctt ggtattaaac agctcaaacc agatccctgg acaagtattg
    19921 aagagaagta ccctgttaac tccaagcaca aggcaaaagt tcgcaatttc actacttttg
    19981 gagtgtttgt tgagttagag gaaggtgttg atggcttaat ccacatttct gaccttagct
    20041 ggaccaaaaa gattaagcat cctgctgaat tcaccaacat tggcgatgaa attgaggtgg
    20101 ttgttcttga aatcgacaag gaaaaccgtc gcttaagtct tggtcacaag cagcttgagg
    20161 aaaacccatg ggatgttttt gagaccattt tcaccgttga ctccgttcat gaaggtacaa
    20221 tagttgaaat tttcgacaaa ggcgctgtaa ttgccttacc atacggtgtt gaaggttttg
    20281 ctactcctaa acatttggta aaggaagatg gttcaactgc taaggttgac gagaaattag
    20341 cctttaaggt gctggaattc aacaagagcg ctaaacgcat aatcgtttcg catagccgta
    20401 cttttgagga tgagaagaag gttgaagaga aagcagaaaa aaaatctaag gcctcttccg
    20461 cacaaaaagt tgtaaaaaag ttgaattcta acatagaaaa gactacctta ggggatattt
    20521 ctgagctgca agctctgaag tcggaactcg aggcaaatca gaaaaaatct gattccaaga
    20581 gcgaagagta ataattgagt tgcgaaagtt taaccattta atcctgaaag aaatggaatt
    20641 caaaattcag aaattcgaga agttcaccca gattgaggtg cttgaagaaa aacttgatac
    20701 caatattgca cccagtttaa aatctgagtt ggttttaatc tcaggcaatg gtgagcgtaa
    20761 cattgtactc gatctgagca gttgtcgata ctgcgattct tcagggttaa gtgctattct
    20821 ggttgcaaac aggctctgta aaaatgccgg tggaactttt gtgcttaccg gacttaccga
    20881 tgccgttgaa cgtttaatta ccatttcgca actcgataca gtgcttaata ttgcctattc
    20941 aaaggatgag gcagtgcaaa taattgagaa agccgaaaac gaagctaaaa agtgacgttt
    21001 tcagttacca tactgggtag tagttctgca ttacctacat ctaaacgttt cccatccgct
    21061 catgtgctca atgtacatga gcggttgttt ttaattgatt gcggcgaggg aacccaaata
    21121 caacttcgca ggtttaagct cccaatgaac cgcatcaacc atatctttct tacccattca
    21181 catggcgacc atgtgctggg cattttcggg ttaatatcaa ctttcaatct tttagggcgc
    21241 aagtacgatt tacatgttta cggcgtagcc gattttgagg agatattcta taagaatatc
    21301 gactacttta ctgaccaaat gtcatttaag gtcatttacc acgttgttga tccttttaag
    21361 gttgaaacaa tttattccga taagcatgta gttgtaacaa ccttgccctt gcggcatagg
    21421 gttccaacgg tggggtattt gttccgcgag gccccacgac ccttgaatat acgtaaggag
    21481 ctaatagcaa agtacaacct ttcgcttact gacattgtta aaataaaaaa tggttcggac
    21541 tatactttaa acgatggaac tgttattcca aatgctgagt tgacctatat ttcatcgcta
    21601 ccccggtcct atgcttactg ctccgatacg ctttacagcg aaaggcttgt tggactagtg
    21661 aaaaacgttg acctacttta ccacgaagcc acctttttac actcggattt acgattagct
    21721 cggttaaccg gtcatagtac cgcacagcag gcagcaacgg ttgccttaaa ggcaagcgta
    21781 gggaaattgc taattgggca cttctcatcg cggtacaaga atgaaaagga tttgcaaagg
    21841 gaagcaatgg aaatattccc agcaacagaa gttgtaaacg acggcctcac cttttcggtt
    21901 gaaccaaaat caacctatta atcaatt
//
LOCUS       DAOSAL010000042        21405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_1745_length_21405_cov_55.929321, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000042 DAOSAL010000000
VERSION     DAOSAL010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 21405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21405
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1745_length_21405_cov_55.929321"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..231)
                     /locus_tag="PK049_07130"
     CDS             complement(106..231)
                     /locus_tag="PK049_07130"
                     /inference="COORDINATES: protein motif:HMM:NF028380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein THX"
                     /protein_id="HOK61368.1"
                     /translation="MGKGDKKTRRGKIFLGSFGNTRPKKSKVRKEKRDKAKVQAK"
     gene            431..841
                     /gene="mce"
                     /locus_tag="PK049_07135"
     CDS             431..841
                     /gene="mce"
                     /locus_tag="PK049_07135"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HOK61369.1"
                     /translation="MSISHIEHIGIAVKSLEESIPFYEKVLGLKCYNIEEVKDQKVKT
                     AFFLVGQTKIELLESTDPEGPIGKFIEKKGEGIHHIAFAVKDIEEQLKRMEEQGVTLI
                     DKAPRKGAEGLDIAFLHPKSTLGVLTELCEDKNK"
     gene            870..2432
                     /locus_tag="PK049_07140"
     CDS             870..2432
                     /locus_tag="PK049_07140"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009128927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOK61370.1"
                     /translation="MSTQEQKIKELIDLREKAKLGGGPKRIEAQHKKGKYTARERIDM
                     LLDEGSFEEFDMFVSHRCIDFGLDKESYLSDGVVTGYGTIDGRLVYVFSQDFTVFGGS
                     LSEMFAAKICKIMDMAMKVGAPVIGINDSGGARIQEGVKSLGGYADIFQRNILASGVI
                     PQISAIFGPCAGGAVYSPALTDFIIMSRNTSYMFVTGPKVVKTVTGETVSSEELGGAG
                     VHSTKSGVAHFVSETEEEGIMLIRKLLSYLPQNNLEEPPLMPCNDPIDRLEDALNEII
                     PDSPNKPYDVKEVIGAIVDNGEFLELQRDYAPNIVTCFARFNGQSVGIVANQPKYLAG
                     VLDINASRKAARFVRFCDAFNIPIVTLVDVPGFLPGTGQEYNGIIIHGAKLLYAYGEA
                     TVPKVTVILRKAYGGAYDVMSSKHLRGDINYAWPSAEIAVMGPKGAIEVLLSRELETI
                     EDEKAKVEFLLRKEQEYKEKFANPYVAAKFGYLDDVIEPRNTRFRIIRALQSLATKKD
                     TNPPKKHSNLPL"
     gene            2450..3367
                     /locus_tag="PK049_07145"
     CDS             2450..3367
                     /locus_tag="PK049_07145"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family transporter subunit"
                     /protein_id="HOK61371.1"
                     /translation="MMKIKRLILATVLVVLGVVIVNAQSVYDLRINEVMLTNKTNYID
                     SYGERSAWIEIVNTGYNSVNVAGCFLTNDISNPMKYRIPSADPITLIPQQQYLVFFAD
                     GKSVHGTLHLNFTLDSINRFVALIAPDGRTLIDSVTLPAIGIDKSFVRLPNGKGEWRI
                     SELATPNSTNELFETHVSTGERFVKFDPFGVIMAIIAMSVVFIALLVLYRLFKLTARL
                     VQKPIRIEFRKSKVAKAEAVEEVAEEVPGEVFAAISAALYFYETEKHDHESTVLTIER
                     VSRRYSPWSSKLYGLQQTPNRIIVHRKLN"
     gene            3392..3829
                     /locus_tag="PK049_07150"
     CDS             3392..3829
                     /locus_tag="PK049_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOK61372.1"
                     /translation="MKDFKFKINGNEYVVHISNVDGNIAELEVNGTPYRVELDKELKQ
                     TKTPKLIRPEAVPATDSHPAVAKTVSPGSATSGAVKSPLPGVILDVLVKPGDHVKVGQ
                     RLIVLEAMKMENNIDSDKEGKVVEVKVRKGDSVLEGDTLVIIG"
     gene            3832..4995
                     /locus_tag="PK049_07155"
     CDS             3832..4995
                     /locus_tag="PK049_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005847422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOK61373.1"
                     /translation="MEQSGSFFSFLLDNLTQFLSFTGFANLTVGHGIMIVVGLLFIYL
                     AIAKEFEPMLLVPIGFGIIVGNIPFTPGYQIGVYESGSVLNYLYFGVLKGIYPPLIFL
                     GIGAMTDFSALISNPKLMLIGAAAQLGIFGAYMLALLLGFSPQEAGAIGIIGGADGPT
                     AIFLSSKLAPELMGAIAISAYSYMALVPVIQPPVMKLLTTQKERLIRMKPPRQVSKLE
                     KIMFPIIGMLLTTFIVPSGLPLLGMLFFGNLLKESTVTKRLADTAKGPMIDIVTILIG
                     LTVGASTQATTFLTPKSVGIFALGAASFVVATFGGVMFVKFLNLFLKEGNKINPLIGN
                     AGVSAVPDSARVSQIVGLEYDKTNHLLMHAMGPNVAGVIGSAVAAGILLSFLA"
     gene            5127..5654
                     /locus_tag="PK049_07160"
     CDS             5127..5654
                     /locus_tag="PK049_07160"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOK61374.1"
                     /translation="MDISGQLVEQCRRGDSKAQFELYKLYANAMFSVSCRIIPDKMEA
                     EDAMQEAFFKAFDKIDTFRNEVTFGAWLKRIVINTCIDHLKRKRLLTVSIDDTPGVSA
                     QASDDDYSPESVEEVKVAMQKLPEGYRLVLTLHLIDGYEYDEIAEMLGIAQSTVRSQF
                     VRAKQRLIELVKNRK"
     gene            5662..6177
                     /locus_tag="PK049_07165"
     CDS             5662..6177
                     /locus_tag="PK049_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61375.1"
                     /translation="MSNIERFIRENSKEFEFQEMLPGHEERFMQRLKQKHRSLTVRTY
                     LMAAATVAVIVAITGAISLYYNYHYGADIARYFTSPESKQLYEVEAYYRSQLLRKYRS
                     IEKIAHVDEQMLPSPEQLFGESLADKPLLKSEISANPRGDVALGAIIQSYQIRLETME
                     RVEQALKQARE"
     gene            6207..7283
                     /locus_tag="PK049_07170"
     CDS             6207..7283
                     /locus_tag="PK049_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61376.1"
                     /translation="MKSIRFFPVVLLLLVANSVLAKVIPQTREYSKEFQVTDKSKLVV
                     ENRFGQVNIQNWDKSAISIYVQVKVDNSNATRAKALLDAIEVELSQEGDVVKAITSFN
                     EEFIRSNRRMFKMSSDELSIDYTIKMPKNVDVQIENKFGDIYINELNSHLAVELKYGN
                     IKIDRLTRGDSESLNTIDVSYGNASIVEANWIKTQLKYGKLEVQKLVAGVVLSRYSKI
                     SLSNVSSLVLDSKYDKYEIGQVNNLVGESGYTVFNIDVLNKKFNLNAKYGDVKIASVD
                     ANFENISFNAAYTGLKASIPSGTSYLLNANVSFGKVDIGNVPSKLNRIEGNTTVELSG
                     VVGESANPKARVEINMKYGNATLY"
     gene            7405..8217
                     /gene="pgeF"
                     /locus_tag="PK049_07175"
     CDS             7405..8217
                     /gene="pgeF"
                     /locus_tag="PK049_07175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HOK61377.1"
                     /translation="MPLIYKDILYFKFDNLMQYQSAITHFVSSRIGCWDDEDFTIGLN
                     GYLDNKIVLRNREYLATKFGFKAADFVFAEQVHGVKVEVVTPMQKGKGAFSKNDALQH
                     SDAWITNYPGICLVAQAADCVPILFYDPVNNAIGAAHAGWKGTVNRIAANVVKSMISE
                     YGTNPANLLVGIGPSAGPCCYEVGDDVASVVNKEFPQKYDLLLYYPDREKPVLDLWRA
                     NLYALLEVGVPVGNIELAGLCTICNNHVFFSARCGDRGRFGGAIMLNVLGQN"
     gene            8400..10334
                     /locus_tag="PK049_07180"
     CDS             8400..10334
                     /locus_tag="PK049_07180"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOK61378.1"
                     /translation="MQATRYIIVILTLVLCQLSVNAQSVKKKYTISNKRVIEQYEKAL
                     SSFDRYDYITAKKLLVECVQKEPKFIEAYLVLSQVYMGMGELNLAIESILKAQSIDKD
                     FHPRAYYILGLLYFSTGEYQKALNSFETFLTYKDKRATMNDSARKMIEKCQFALNQIA
                     NPVPFVPKNIGEGVNSDLDEYWPSLSVDERTLVYTVRLPKNPDKGIKGTKWQEDLYIS
                     YRNEDGQWGRGEPVKSPLNTEFNEGAQAVSSDGKTIYFTICRGVCNLYQSVLEPDGFW
                     SLPQKLPAAINSERYSEKQPSISPDGKTLYFVSNRPGGLGKFDIWKSELKPDGTWGNP
                     VNLGDVINTNESEQSPFIHFDNKTLYFSSSGHMGMGGHDIFVSRLDSNGAWTKPVNLG
                     YPINTHRDEDGLIVNARGNIAYYSTDIKPENGRDIFTFELYPEVRPTPTSYVLGTVRN
                     SKTLEPVEASIKLVDLKLDRVVMDIVSALKGEFLVCLPVSNRYGLFVSASGYLFHSEH
                     FDLTEYYSFDKPLKLDVLLQPIEKDEVITLRNIFFAFDSYELLPESRPELNYLLDILK
                     RNPEMRIEISGHTDDTGSAEYNLKLSEQRAKAVADYLISQGIDSARLKWVGYGKSKPV
                     APNSTPEGRTLNRRTEVKIL"
     gene            10503..12395
                     /gene="speA"
                     /locus_tag="PK049_07185"
     CDS             10503..12395
                     /gene="speA"
                     /locus_tag="PK049_07185"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008767598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HOK61379.1"
                     /translation="MRKWRIEDSAELYNINGWGVKYFSINEKGNVVVTPKSNGVEIDL
                     RELVDELQVRDVAAPMLIRFTDILDNRIEDMSRSFKQAAEEYGYQGKNFIIYPIKVNQ
                     MRPVVEEIINHGKKFNIGLEAGSKPELHAVIASNPDNESLIICNGYKDEDYIELALLA
                     QKMGKKIFIVVEKLNELKVINKVSQRLKIKPNLGIRIKLASSGSGKWEESGGDVSKFG
                     LTSSELLQALDFLDKNDLKGSLKLVHFHIGSQVTKIRRIKIALREASQFYVQLRLMGY
                     NVEFVDIGGGLGVDYDGTRSSNSESSINYSLQEYVNDSVSTLVDASDKNNIPHPNIIT
                     ESGRSLTAHHSVLIFEVLETASLPSWDEDDELPADAHELVVELYEQWSKLNQSRMLET
                     WHDAQQIREEALDLFSLGMLDLKTRAQIEKLFWSIARETFEMTSQLKHIPDELKSLPK
                     LLADKYFCNFSLFQSLPDAWAIDQVFPIMPIQRLNEKPDRVATIQDVTCDSDGKIDNF
                     ISTKNISYFLPVHSIKSNDSYYIGVFLVGAYQEILGDLHNLFGDTNAVHVSVDDEGYS
                     IDQIIDGETVAEVLEYVQYNPKKMVRTLETWVTSSVKAGVISPKEGKEFLSNYRSGLY
                     GYTYLE"
     gene            complement(12749..12973)
                     /locus_tag="PK049_07190"
     CDS             complement(12749..12973)
                     /locus_tag="PK049_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61380.1"
                     /translation="MGTPWVELPRDTIRHACGVTHVVSLTRPTATFHPRPIAGSGFYA
                     TAYGTPCWLRNRIKEELKVLRDASTGLSMT"
     gene            complement(13613..14989)
                     /locus_tag="PK049_07195"
     CDS             complement(13613..14989)
                     /locus_tag="PK049_07195"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOK61381.1"
                     /translation="MGKNHLSVLASTVIAAISITCHAQVRVELKVHQSIGSTAIVSRY
                     EGKNQIEVDSCKPTPDGVYTFSVPADTPKGIYKVSVGKGASFDFIVSTDTIVRFETYS
                     FAVEDSLKVKQSAENEVFINFRKLRQKAEQKMWLIESLRKYYTEPTMFSQMLENEHQR
                     TAFDLYMQGNALASKANSSLVSSAIRLELTPQPITQGDECSVKKELSEIWWQGIDLTN
                     PDIHFLPRLIPRLWDYLENLLCEGNYTKEEQDSVLSEYIQKLFNLPMHSEIKSLLLSS
                     LCNGFAESDYYGVISTLQQLNENKICPIFNDPELKAKLVLEAGLMPGKKAFDFTFKPV
                     GQKKSIKLSNSTSKYTLVLFWSVWCPHCIESVPQIYKTYKQYQGKGFDVIAICIDDED
                     DAFQNFIKQNELHWKNIRIPYDSNSKVILKYNVDETPKMFIVDKKLNILSRPSTPEHV
                     KVFLEKNL"
     gene            complement(14998..18213)
                     /locus_tag="PK049_07200"
     CDS             complement(14998..18213)
                     /locus_tag="PK049_07200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOK61382.1"
                     /translation="MRDFATVSMSSDSQNNNSYSNWDNFDSNFLIPLIFQNESSIYLI
                     QNFRVVFANPSFTKLTGYAQDELNEILFLDVVHPKDRKLVKILFNNDFAEIRKHTSNS
                     FTLRILTRYNELKWIKTIFSIIDWQESPALLCTSYDITQQKEAEESMANQEQNLSMLV
                     NAFGDLVFIVNHNYNIVQINNAVLSALGHHEHEILLESFVKLHAESERGIAITSLMEV
                     FDGDRKLYITEFVRKNGKPLPLEVRMIKGYWRKRDVVFVIARDITERIEAEAAIKQSE
                     EKFFKAFNSGAVMMTISTLNEGTYIDANRAFLEKIGYDYSEVVGQKSSELRIFKQIER
                     RNELIDAVKRENRVDKIEVEIVNKKGETFTTLLSAEIINIQGTDCLLVAMSDITYRKQ
                     VEEELARSRAQLRGTIDNLPFIAWLKDSKCGYLLVNKKFTNHFGITDDQILGKVDTEP
                     WPSPLLDTLKQKENEVLKSKQTVKWEIREGERRIEEWWEFQLTPVFNVNKKVIAITGI
                     ARKITDQKINQIKIQKHLDRQILLTKVSYVFNKNLPFSKQANESLNLIVSEIGLRKAF
                     ILIGSESDFDIFTCCSSTANDLAEPLRVYYSDNYTSITSHFQQENKVIFDISGSTMPS
                     YDNLKKLTSSQHLLLFPIRVKEKCQGIFAVDYPYGDKVKAADDRDFLLTMSNILSASY
                     ESHLNEEQLRKAKELAEKASQAKEKFLSTMSHEIRTPMNAIIGMANLLIDEDPKPEQV
                     DNLNSLKYAAENLLSLLNDILDYSKIEAHKLDLLQSNFDINDLFKGLYSTFAKMASSK
                     GLTLTYNIDTNIPTPLIGDRVRLNQVLTNLIGNAIKFTEKGEISFTAKLVEKNKEIST
                     IRFTVSDTGIGIPKKMQVDIFNEFTQVHEGKFRATGTGLGLAISQRIVKLMGGEISLE
                     SDEGKGSSFFFTIPFKVGTVQPTDDTVEINIPPGKHRVLIVEDNELNTFIVKRFLTNW
                     GIDFEHASNGREALSCLAQNDFDLILMDLEMPEMNGYEAAKAIRKLHNKAKASIPIIA
                     LSASALLNVQQRIFGIGMNDFVLKPFKPQELKSKLAKYLLK"
     gene            18418..18894
                     /locus_tag="PK049_07205"
     CDS             18418..18894
                     /locus_tag="PK049_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61383.1"
                     /translation="MIIKKIAFYIVIATIFFFLTNGSCDKTECGEFWFINRTTHRVKI
                     NSYTYFGLITDMSYEIQQIDSISKVTCDMGMPSNPFTSNYFQVDSIVIVFDDTLSINI
                     FKYLEYNPMNFKVRKKNKFVYEITDSLYCKTLEINGYSSKIVSLPVNGRGTLTQFE"
     gene            18976..19398
                     /locus_tag="PK049_07210"
     CDS             18976..19398
                     /locus_tag="PK049_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61384.1"
                     /translation="MKAYKLYLFAVIVSFLNSCGDKDVCEDYRFENATDHRVYIRSCC
                     GEATDSKTSLSIGPKSSATLTSCGLGYAQFVYNGVSRCAVDKVIIIFDDTLEFNTQEF
                     WEFDLLNPLNYQRRNKEYIYEINQLHYQKALEVNGYLK"
     gene            19443..20123
                     /locus_tag="PK049_07215"
     CDS             19443..20123
                     /locus_tag="PK049_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61385.1"
                     /translation="MNSDNRFEMSTPYTPSVLKPKLKVGYYHHDHWRDINGSAVPFRE
                     NLTIPHVCIYGKDGSGSWSTTDFVYATTCHEVVHVSHWEMIGEGAFAILWLNPKTRII
                     PESWAVAVEWGLTNLEYKVLGQKYLSPQALSYNFNYGYQSWYLGRNDFYTPLFIDLID
                     DFNQKTYYNGDNRPDDKVKNYTLSSIESILFGVRDYEVLKAMLKMNKPVGVTDGDIDD
                     LINYYRNI"
     gene            20137..20556
                     /locus_tag="PK049_07220"
     CDS             20137..20556
                     /locus_tag="PK049_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61386.1"
                     /translation="MKTFFKLLISVGLIVIFSHASCDKWVCGDNWFVNKTMHKINVNV
                     YCHGEANNELSFHIAPYDSINRMTCGLGGPVDPFTSNTCGPDSILVVFNDTIPLNTFR
                     YLEYNPMNLKKIGKTKFRYEFTEMHYQKALEVNGYLR"
     gene            20625..21260
                     /locus_tag="PK049_07225"
     CDS             20625..21260
                     /locus_tag="PK049_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61387.1"
                     /translation="MSTPYTPSVLKPKLKVGYYHHDHWRDINGSAIPFRENLTIPHVC
                     IYGKDGSGYWSTTDFIYATTCHEVAHVSHWEMVGEGAFALIWLNPKTRIIPESWAEAV
                     SWQLTRNEYKRFGDFALSSADFNFSKQVWSNNMNKYYTPIFIDLIDNINQRVKYSGLL
                     NYPNDNVTGYTVAKLEQLLYAFMDLDLLEATLLVNKPSGVTNESIKELGFL"
BASE COUNT         6095 a         4178 c         4758 g         6374 t
ORIGIN      
        1 caatttttga ttaaattgca ctatgcgtta acatagtgtt ggtttgaggt gataaataaa
       61 aaaaaccggc tgtgccggtt tgagtcattc gttagtaagt tcgctttact ttgcttggac
      121 tttagcctta tcgcgttttt ccttacgaac ttttgatttt tttgggcggg tgttgccaaa
      181 cgaacccaaa aatattttac ccctgcgggt ttttttatcg ccttttccca tggtgttata
      241 gtattagttt attactcaaa gttggctgca aaaataagcg attattttat tttatgttcc
      301 attttcggca aaattctaac aaacatgttc tttaacaatg ctgaaaaaca actaaaacgt
      361 ttgaaacttt tatggttaaa gtaaactata caaagtaact ttgcacaaac taatttaaaa
      421 taatacagct atgagcatat cacacattga acacatcggt atagccgtta aaagtcttga
      481 ggaatcgatt cctttctacg aaaaggtact gggtttaaag tgttacaata ttgaagaggt
      541 aaaggatcag aaggttaaga cagccttttt cctggttggc cagaccaaaa tagaattgct
      601 tgaatctaca gaccctgaag ggccaatagg aaaattcatt gagaaaaagg gtgagggtat
      661 ccaccatatt gcttttgctg taaaagatat tgaggagcag ttaaagcgca tggaggagca
      721 gggagtaacc cttatcgata aagctccccg taagggtgct gaagggcttg atattgcttt
      781 ccttcatccg aagtcaaccc tgggcgtatt aactgagctt tgcgaggata aaaataagta
      841 aaaaacatcc ccaaacacca aatataacga tgagcacaca ggagcaaaaa atcaaagaac
      901 ttattgattt aagggaaaaa gcaaaacttg gcggaggccc caagcgtatt gaggctcagc
      961 ataaaaaagg aaaatacaca gcacgcgagc gtatcgatat gctgctcgat gagggtagtt
     1021 ttgaagagtt cgatatgttt gtttcgcacc gctgtatcga ttttggtctc gataaggaga
     1081 gctacctatc tgacggtgta gtaaccggct acggcaccat tgatggccga ttggtttacg
     1141 tattttcaca agattttaca gtgtttggag gctcactatc ggagatgttt gccgctaaaa
     1201 tctgcaagat catggatatg gccatgaaag ttggtgctcc ggtaatcggt atcaacgata
     1261 gcggcggcgc tcgaattcag gaaggagtaa aaagtttagg cggatatgcc gatatttttc
     1321 agcgtaacat tttagcctcg ggtgttatac cccagatatc agccattttt ggtccatgcg
     1381 ccggtggggc ggtttattca cccgctctta ctgacttcat catcatgtcg cgtaatacca
     1441 gctacatgtt tgttactggt ccaaaggtgg ttaaaactgt gaccggtgag actgtttcat
     1501 ccgaggaact tggtggtgct ggggttcatt ccacaaaatc gggggttgca cattttgtat
     1561 ccgaaaccga ggaggaaggc attatgctta tccgcaagct cttgagctac ctgccccaaa
     1621 acaaccttga ggagccaccg ctaatgccct gcaacgatcc tatcgatcgg ttggaggatg
     1681 ccctaaacga gattatcccc gatagcccaa acaagccata cgatgtaaaa gaagtaattg
     1741 gagctattgt tgataatggc gagttcttgg agttgcagcg cgactatgca cccaatattg
     1801 taacctgttt tgcccgtttc aacggacagt cggtgggaat tgtggccaac caacccaaat
     1861 accttgccgg tgtgctcgat atcaatgctt cccgcaaagc tgctcgcttt gtaaggttct
     1921 gcgatgcatt caatattcct atagttacac ttgttgatgt tccgggcttt ttgccaggta
     1981 ccggccagga gtacaatggt atcataatac atggagcaaa acttctttac gcctacggcg
     2041 aggctactgt tcctaaggtt acggtaatat tacgtaaagc ctacggcggc gcttacgatg
     2101 tgatgagctc aaagcacctg cgtggcgata tcaactacgc ttggccaagt gctgaaatag
     2161 cggttatggg acccaagggt gccattgagg tattacttag ccgtgagttg gaaaccattg
     2221 aggatgaaaa agctaaggtt gagttcctgc ttcgcaagga acaggagtac aaggaaaagt
     2281 ttgctaaccc atacgtagca gccaagtttg gttacctcga cgatgtgatt gaaccgcgca
     2341 acacccgctt ccgcataata agggcgttac agtcgttagc aaccaaaaag gataccaacc
     2401 caccaaagaa acactctaac ctaccgttgt aaccaaaatg attgtagcaa tgatgaaaat
     2461 aaaaagattg attttggcta cagtccttgt agttctgggg gttgttatcg taaacgcaca
     2521 aagcgtttac gacctccgaa ttaacgaggt gatgttaact aacaaaacga actacattga
     2581 tagctatggt gagcgaagtg catggattga gatagtaaat acgggctaca atagtgttaa
     2641 cgttgcaggc tgtttcctta ccaacgacat ctcaaacccc atgaaatatc gtattccatc
     2701 ggccgatccc atcacgctca taccgcaaca gcagtatttg gtgttttttg ccgatggtaa
     2761 aagcgttcat ggtacattac acttgaattt tacacttgac agcatcaacc gttttgtggc
     2821 acttattgca cccgatggcc gtactctgat tgatagtgtt accttacctg caattggtat
     2881 tgataagtcg tttgtacgat tgcctaacgg aaaaggggaa tggcggatta gcgaacttgc
     2941 tactccaaac tctaccaatg aactttttga gactcatgta tcaactggtg aaaggtttgt
     3001 aaagtttgac ccctttggcg taattatggc aatcatagct atgtcggttg tgttcattgc
     3061 tttactagtg ctataccgtc tttttaagtt gactgccagg ttggttcaaa agcctattcg
     3121 tattgaattc cgaaagtcaa aagttgccaa ggcagaggca gtggaagaag tggctgaaga
     3181 ggttccgggt gaggtttttg cagccatatc agctgcgcta tacttctatg aaaccgaaaa
     3241 gcacgaccat gaatcaactg tacttaccat tgagcgggtt tcgcgccgct attcgccttg
     3301 gagttcaaaa ctttatggtt tgcaacaaac tccaaatcgc attattgttc ataggaaatt
     3361 gaattagtta aaacgctaac aatcactcaa aatgaaagat tttaagttca agataaatgg
     3421 caacgagtat gtagttcaca tctcgaatgt tgacgggaac atagcagaac tcgaagtgaa
     3481 tggaacgcca tacagggtgg agctcgataa ggagcttaag caaactaaaa cgcctaaact
     3541 tattcgacct gaggctgtgc ctgcaaccga ctcgcatccc gcagtagcta agacagttag
     3601 ccctggttca gctacctcgg gtgctgttaa atcgcccctg ccaggcgtta tactcgatgt
     3661 gcttgttaag ccgggtgatc atgtgaaagt gggacagcgc ttgatagtac ttgaagccat
     3721 gaaaatggaa aacaacatcg attccgataa ggaagggaaa gttgttgagg ttaaggttcg
     3781 taagggcgat tcggttcttg aaggtgatac acttgtaata attggttagc catggagcaa
     3841 agcggaagtt ttttctcgtt cctgttggat aacttaaccc agttcttatc ctttaccggt
     3901 tttgccaacc tcactgttgg ccacggtatt atgattgttg tggggttgct gtttatttac
     3961 ttagccattg ccaaggagtt tgagcccatg cttctggttc caattgggtt tggtattatt
     4021 gttggaaata ttccattcac tccgggttat cagataggag tttatgaaag tggaagcgtt
     4081 ttaaactacc tatactttgg tgtacttaaa ggaatttatc caccgctgat attcttaggt
     4141 ataggggcta tgaccgactt tagtgcgcta atttcaaacc ccaagcttat gttaattgga
     4201 gctgctgctc agctgggtat ttttggggcc tatatgcttg ccctgttact tggcttttcg
     4261 ccgcaagagg ctggtgcgat tggtattatt ggtggtgccg atggaccaac ggcaatcttc
     4321 ctatcgtcaa agttagcccc ggagcttatg ggtgctatag ccatatcggc atactcatac
     4381 atggcgttgg tgcctgtaat ccagccaccc gtgatgaaac tccttactac acaaaaagag
     4441 cgtttaatcc gaatgaaacc acccagacag gtttcaaagc ttgaaaaaat tatgtttcca
     4501 attattggaa tgctgctcac cactttcatt gtaccctcag gcttaccatt gctgggaatg
     4561 ctcttctttg gcaacctgtt aaaggaaagt actgttacca agcgtttggc cgatacagcc
     4621 aagggcccaa tgattgatat tgtaactatt ctgattggtt taacggttgg cgcatcaacc
     4681 caggctacta ctttccttac accaaaatcg gttggtattt ttgctctggg agcagcatcg
     4741 ttcgtggtgg caacctttgg tggtgtgatg tttgttaagt ttttaaacct attcctcaag
     4801 gagggaaata aaattaaccc attaattggt aatgccggag tatcggctgt tcccgatagc
     4861 gcccgcgtat cgcagatagt tggtttggag tatgataaaa ccaaccacct gcttatgcat
     4921 gccatgggtc ccaatgttgc cggggttatt ggtagcgcag ttgctgctgg tatcctgctc
     4981 agcttccttg cctaacatat aagtatccga tgatataaag ccccgttacc ggggcttttt
     5041 ttgttttttc aatttttttt attccgcaat gcagcaaaag gcattaactt gcagtctata
     5101 tagtagattt tggttttatc ggctttatgg atatttcagg gcagctagtt gaacagtgtc
     5161 gacgtggcga cagtaaagct caatttgaac tttacaagct atatgccaat gccatgttta
     5221 gcgtgagctg caggattatc cccgataaaa tggaggccga agatgccatg caagaagcgt
     5281 tctttaaagc attcgacaag attgatacct tcagaaatga ggtgactttt ggggcttggc
     5341 ttaagcgtat tgttatcaat acctgcattg accatttaaa acgcaagcgt ttgctcaccg
     5401 tttccattga cgatactccc ggggtatcgg ctcaggcaag cgatgacgac tatagccccg
     5461 aatcggttga agaggtaaag gttgccatgc aaaagctgcc cgaaggctac cgattggttc
     5521 tcacccttca ccttattgat ggttatgagt acgatgaaat tgctgaaatg cttggtattg
     5581 cccaatcaac tgtccgttca cagtttgtaa gggccaaaca aaggctgata gagttggtta
     5641 aaaacagaaa gtaggtttgc tatgtcaaat attgagagat ttatcaggga aaacagcaag
     5701 gaattcgaat tccaagagat gctaccaggg catgaggagc gcttcatgca aaggttaaaa
     5761 caaaagcatc gctcgctaac cgttcgtacc tacctaatgg ctgctgctac tgttgcggtt
     5821 attgtagcaa ttacgggggc aattagcctt tactacaatt accattatgg tgccgacatt
     5881 gctaggtact tcaccagtcc tgagtcaaaa cagctttacg aggttgaggc atattaccgt
     5941 agccagttgc tcaggaagta tcgctcaatt gagaaaattg ctcatgtaga tgagcaaatg
     6001 ctgccctcgc ccgagcaact atttggtgaa tcgctagccg ataagcccct gctaaaatct
     6061 gagatatcag ctaatccaag gggcgatgtt gccttgggcg caatcattca gagttaccag
     6121 attcgactgg aaaccatgga acgcgttgaa caagctttaa aacaggctcg agagtaaaaa
     6181 agtagaaatc attaaactca aacaatatga aaagtattag gttttttccg gttgttctgt
     6241 tgctacttgt tgctaatagc gtattagcaa aagtaatccc tcagaccagg gagtatagca
     6301 aggaattcca ggttaccgat aaatccaaat tggtggttga gaataggttt ggtcaagtaa
     6361 acatccagaa ctgggataaa agtgctatat caatttacgt gcaggttaag gttgacaatt
     6421 caaatgcaac cagggccaaa gccttactcg atgccattga agtagagctt agccaggaag
     6481 gtgatgtggt aaaggccatt acctcattca atgaggagtt cataaggtca aaccgcagaa
     6541 tgtttaaaat gtcgagcgat gagttgtcca ttgactatac aattaaaatg cccaagaatg
     6601 ttgatgttca gatagagaat aaatttggcg atatctatat caatgagcta aacagccatc
     6661 ttgcagtaga gctgaagtat ggtaatatca agattgatcg gctaacccgg ggagactccg
     6721 aatccctcaa taccattgat gtcagctatg gtaatgcatc tattgttgag gccaactgga
     6781 ttaagaccca gttgaagtat ggtaaactcg aagtacagaa actggttgca ggggttgttc
     6841 tatcgcgata ctcaaaaatt agcctgtcga acgtaagttc gttagtattg gattccaagt
     6901 acgataagta tgaaattgga caggtaaaca atctggttgg tgaatcggga tatacggttt
     6961 ttaatatcga tgtcctcaat aaaaagttta acctcaacgc caagtatggt gatgttaaga
     7021 ttgcatcggt agatgctaat tttgaaaaca tcagctttaa cgctgcctat accggattaa
     7081 aagcttcaat tccatctgga acatcttatt tgctgaatgc aaatgtgtcg ttcgggaagg
     7141 ttgatattgg taacgtacca agtaaactta atcgtattga ggggaataca acggttgagc
     7201 tatcgggggt tgtaggcgag tccgctaacc caaaggcaag ggttgaaata aacatgaagt
     7261 acggtaatgc aacgctctac tagtgtccca ccattcatac ccaaaaccta acagttaggt
     7321 gctaaaatcc gacagttcgt cggattttat tttttattgg tggccgattt ttagttactt
     7381 ttgttacata ccaaaaaaca gcgaatgcct ttaatctaca aagatatttt gtacttcaag
     7441 ttcgataatt taatgcaata ccaatcggca atcactcact ttgtttcttc gcgcattggt
     7501 tgctgggatg atgaggattt tacaattggc ttgaatggtt acttggacaa taaaattgtc
     7561 cttaggaata gggagtacct tgcaaccaag tttggattta aagcagccga ttttgttttt
     7621 gctgagcagg tgcatggggt taaggttgag gttgttacgc caatgcaaaa ggggaaaggg
     7681 gcttttagca aaaacgatgc ccttcagcat tccgatgcat ggataaccaa ctacccggga
     7741 atttgtcttg tagcccaggc tgccgattgc gttcccattc ttttctacga cccagtgaat
     7801 aatgcaatag gtgcagccca tgcgggctgg aagggaacgg ttaatcgaat tgcagcaaat
     7861 gtggtaaagt ctatgattag cgagtacgga acaaaccccg ccaacctttt agtaggaata
     7921 ggcccttcgg cagggccatg ctgctacgaa gtgggcgatg atgttgcttc agttgttaat
     7981 aaagagtttc cccaaaagta cgatctttta ctatactatc ccgataggga aaagcctgtt
     8041 cttgaccttt ggcgtgcaaa cctttatgca ttattagagg ttggtgtacc tgtgggtaat
     8101 attgaactag ctggtttatg taccatttgc aataaccatg tattcttctc ggctcgctgt
     8161 ggtgatagag gtaggtttgg cggtgccata atgcttaatg tgcttggcca gaattaacct
     8221 ttttctattc ttattgctgg aagtggtttg ggttgaactg tacatcttga atagccagga
     8281 ggatatcact ttaggttctg gtaaattttt cacgcataag ttctaagcta tcaacttggt
     8341 gtcattttcg aaaacaatga ttacctttat ttcgtatcaa aataatatag aggtaagtta
     8401 tgcaggctac acgatatatt atagttattc ttaccctagt gctttgtcag ctgagtgtaa
     8461 atgcacagag tgtcaagaaa aagtatacaa ttagtaataa acgagtaatt gaacagtacg
     8521 aaaaagctct gagttcattt gaccgttacg attatataac agctaaaaag ttgctagttg
     8581 aatgtgttca gaaagagcca aagtttattg aggcatacct tgtgctttcc caggtttaca
     8641 tgggaatggg ggagcttaac ttagccattg agagtattct gaaagcccaa agcatcgata
     8701 aagattttca tccaagagcc tactacattt tggggttgct ttacttttca acaggggagt
     8761 accaaaaggc gctcaacagc ttcgaaactt ttttgacata caaagataaa agggcaacca
     8821 tgaacgattc ggcacgcaaa atgattgaga agtgccagtt tgccctaaat cagattgcca
     8881 acccggttcc ttttgtacca aaaaatatag gcgaaggggt taattccgat ttggatgagt
     8941 actggccaag cctttcggtc gatgagcgta cactggttta taccgttcgc ttgcccaaga
     9001 atcccgataa gggaatcaag ggaaccaagt ggcaggaaga cctttacatt tcctacagaa
     9061 atgaggacgg tcaatgggga agaggagagc ctgtaaaatc acccctgaac accgagttta
     9121 atgaaggagc ccaagcggtt tcgtccgatg gcaagaccat atactttaca atttgtcgcg
     9181 gagtatgtaa tttataccaa tcggttctag aacccgatgg attttggagt ttgccgcaga
     9241 aattaccagc tgccataaat tctgaaaggt attcggaaaa gcagccttcc atatcccccg
     9301 atggtaaaac gctatacttt gtaagtaata ggccgggtgg ccttggtaag ttcgatatat
     9361 ggaagtcaga gcttaagcct gatggtacct ggggaaaccc ggtgaatttg ggtgatgtga
     9421 ttaataccaa cgagagtgaa caatcgcctt tcatccattt cgataataaa actctatact
     9481 tttcatcgtc gggccacatg gggatgggcg gtcacgatat ctttgtaagt cggctcgata
     9541 gtaacggtgc ctggacaaag ccggtcaacc tgggttatcc aattaacacc catcgcgatg
     9601 aggatggctt aattgtaaat gctaggggta acatagccta ctattccact gacatcaaac
     9661 cggaaaatgg acgggatatt ttcacctttg aactttatcc cgaggtgcgt ccaactccaa
     9721 catcgtatgt tcttggtact gtgcgaaaca gtaaaaccct tgagccagtt gaggcgagca
     9781 ttaagctggt cgatttaaag cttgataggg ttgtaatgga tatagtttcc gcattgaagg
     9841 gtgagttcct ggtttgccta ccagtaagca ataggtacgg tttgtttgtt tcggcatcgg
     9901 ggtatctttt ccattccgag catttcgatt tgacagaata ctactctttc gataagccat
     9961 taaagcttga tgttctactg caacccattg aaaaggatga ggtgataacc ctcaggaata
    10021 tcttttttgc atttgactcg tacgagctat tacccgaatc gagacctgag ctaaactacc
    10081 tgttggacat tcttaaacgt aaccccgaaa tgaggattga gattagcggg cacactgacg
    10141 atacgggaag tgccgagtac aacctgaagc tatccgagca gcgcgcaaaa gcagtggccg
    10201 actacctaat ttcgcaaggg attgattcgg caaggctgaa gtgggttgga tatggtaaaa
    10261 gcaaaccggt tgcccctaac agtacgcctg agggtaggac gctgaaccgc agaacggagg
    10321 tcaaaattct ttaggtggct tatggtttta tatgagttga cattatttaa cggttttgaa
    10381 ccatatttct cattactgta ttgtaattgt tgaaataaca atataacttc gcaagcctta
    10441 aaagtttaat agcaagatac tgtatgatag atgtttaact tagcaatatc tggtaaagag
    10501 agatgagaaa atggcgcatt gaggattcag ccgaactcta caatatcaac ggctgggggg
    10561 taaagtactt ttcgattaac gaaaagggaa atgtagtggt taccccaaaa agtaatggcg
    10621 tggagattga cctgcgcgaa ctggttgatg agttacaggt tcgcgatgtg gctgcgccca
    10681 tgctaattcg tttcaccgat attctcgaca atagaatcga ggatatgtcg cgctcgttca
    10741 agcaggcagc cgaggagtac ggctatcagg gtaaaaactt catcatttac cccattaagg
    10801 ttaaccagat gcgccctgtg gttgaggaga ttatcaacca cggtaaaaag tttaatattg
    10861 gactggaggc tggctcaaag ccagaacttc atgcagttat tgcaagtaac cccgataacg
    10921 agtcgctgat tatctgcaac ggttacaagg atgaggatta cattgagctg gctctgcttg
    10981 cccaaaagat ggggaaaaag atatttattg tagttgaaaa gctcaatgag ctgaaggtaa
    11041 tcaataaagt ctcacagcga cttaaaatta agccaaacct tggcattagg attaagttgg
    11101 ctagctccgg tagtggcaaa tgggaggagt caggtggcga tgtcagcaag ttcgggttaa
    11161 catccagtga gctcctacaa gctctggatt ttctggataa gaatgaccta aagggtagct
    11221 taaagctggt tcacttccac attggaagcc aggtaaccaa aatcaggcgt ataaaaattg
    11281 ccctgcgcga ggcatcgcag ttttacgttc agcttcgctt aatgggctat aacgtagagt
    11341 ttgttgatat tggtggtgga ctaggtgttg actacgatgg aacccgctca tcaaacagtg
    11401 agagtagcat taactatagc cttcaggagt acgtaaacga ctcggtatca accctggttg
    11461 atgccagcga taaaaataac attccgcacc ccaatatcat tactgaatcc ggcaggtcgt
    11521 taactgcaca tcactccgtg ctcatttttg aggtactgga aactgcttcg cttccctctt
    11581 gggatgagga tgatgagcta ccggccgatg cccatgaact ggtggtggaa ctttatgagc
    11641 agtggagcaa gctaaaccag tccagaatgc ttgaaacttg gcacgatgcc cagcaaatca
    11701 gggaggaggc actcgacctg tttagccttg gcatgctcga ccttaaaacc cgtgcgcaaa
    11761 tcgaaaagct tttctggtca attgcccgcg aaacctttga gatgacctca cagcttaagc
    11821 atatacccga tgagctaaaa tcgttgccta aactcttagc cgataagtat ttctgtaatt
    11881 tttcgctgtt ccagtcgttg cccgatgcct gggctatcga tcaggtgttc cccattatgc
    11941 ctattcagcg tttaaacgaa aaacccgaca gggttgccac catacaggat gtaacctgcg
    12001 actccgatgg taagattgat aactttattt caaccaaaaa tatttcgtat ttcctaccgg
    12061 ttcactccat caagtccaac gattcctatt acattggagt attccttgta ggtgcttacc
    12121 aggaaatact gggtgacctc cacaacctgt ttggcgatac caatgctgtt cacgtatcgg
    12181 ttgacgatga agggtatagc attgatcaaa taattgatgg tgaaactgta gctgaggtgt
    12241 tggagtatgt acagtacaac ccaaagaaaa tggttcgtac cctggaaacc tgggttacca
    12301 gctcggttaa agctggggtt atttcgccta aggaaggaaa ggaattcttg tccaattacc
    12361 gctcaggctt atatgggtac acttaccttg agtaaatttg catagaatag gatataaaag
    12421 cccacatagg tgggcttttt tcttttctgg tttcaagttc gttagttgaa aaaatcaatt
    12481 gaagaatcga ctaataaaat ctaatcatct cgtaacttcg gaatgaaaga ttttgataaa
    12541 tcgggattat ctcgataatt atgtaatttt tattgtgtat gtattgacta atctcttcaa
    12601 aaagcttaca gtgttcctat tttttgcctc gatgagttag ttaattcaga acacttagat
    12661 tgttaagatt tacactgcat aacaatttga aagtaattta gttgatagca gacaattgtt
    12721 tgttgttagt aaaaacagaa ttcagtcttc atgtcatgct gagcccagtc gaagcatctc
    12781 ttagtacctt caactcttct ttaatgcgat tcctcaacca acacggtgta ccatacgcag
    12841 tggcataaaa gcctgacccg gcaatgggtc gggggtggaa cgtggcggtg ggcctagtaa
    12901 gcgataccac atgagtcacg ccacaggcgt ggcgaattgt atcgcgtggc agctccaccc
    12961 acggggtacc catcgggtcg ggcttttcag ccttgatttt ctttggttct ttcttgtatc
    13021 aagacaagaa agaacgtgta aattgctctt tataatgctt ttcttctgcg atgcaacact
    13081 ttccccttgc tctctcgaaa atgtattgcg tgcatcgcta tacatgtggg aataatcatg
    13141 gagttacaaa tattacgccc ctacagggct ggaaacggtg ctcattgttg ttcgtgaatc
    13201 gttatccgcg aataccgtat cattcactct gtgaaacaac gtgttacatt gtgaaactct
    13261 gtgataccag tttagttgat agttgatggt tgatggttgt tagcagaaga gaatttagcc
    13321 ttgatgtcat gctgagctag cccaagcatc actttgtacc ttcattcact tattaatgtg
    13381 atttctcgat actgcctatt ccgtgtatca ggactagaca ccgtttaacg gttaacggct
    13441 cacggttcac gtttaacgga ctagaaattc ttctaacttc ctctaactcc atcatattct
    13501 tcttattctg attcattccg attcattccg acttattcag gttcattcca cctcattcct
    13561 ctaaaatcag gcaagttgga tttgttaatt catggaactt ctgtttagct aattacaggt
    13621 tcttttcaag aaaaacttta acgtgttcag gcgtagatgg gcgcgataga atattcaact
    13681 ttttatcaac tataaacatt ttaggggttt cgtcaacatt gtattttaaa attaccttac
    13741 tattgctatc gtaaggaatt ctgatattct tccagtgcag ctcattttgt ttgatgaagt
    13801 tttgaaaggc atcgtcctca tcgtcaatgc atattgctat aacatcaaag cctttaccct
    13861 ggtattgctt ataggtttta tagatttgtg gtacgctttc gatgcagtgg gggcaccaaa
    13921 ctgaccagaa tagaaccaaa gtgtattttg atgtgctatt tgatagtttt atcgattttt
    13981 tctgacccac tggcttaaat gtaaagtcga atgctttttt cccgggcatt agccccgctt
    14041 caaggacaag ttttgctttg agttcggggt cgttaaaaat ggggcagatc ttattttcgt
    14101 tcagctgctg cagggttgat atcactccat agtagtccga ctcagcaaaa ccgttacaaa
    14161 gtgagcttaa tagtaacgat ttaatttcgg aatgcatggg caggttaaat agtttttgga
    14221 tgtattccga taaaacagag tcctgttctt ccttggtata gttgccctcg catagcaagt
    14281 tttccaggta atcccataat cggggtataa gtctaggcaa aaaatgtatg tcgggattgg
    14341 taaggtcaat tccttgccac catatttcgg atagctcttt tttcactgag cattcatcgc
    14401 cctgggtaat cggttggggt gtcagctcaa gccttatggc tgacgatacc agcgaactat
    14461 tggcttttga agcaagcgca ttaccctgca tatacaggtc gaaagctgtt cgttgatgct
    14521 cgttttcgag catttggcta aacatagttg gttcagtgta atacttacgg agcgattcta
    14581 ttagccacat tttttgctca gccttttgcc ttagctttct gaagtttata aaaacttcgt
    14641 tctcggccga ttgttttacc tttaagctat cctcaacggc aaatgaatag gtttcaaagc
    14701 gcacaatggt atcggttgaa acaataaaat cgaacgaggc ccctttccct accgatacct
    14761 tgtaaattcc tttaggtgta tctgcaggga cactaaaagt gtaaacacca tcgggggttg
    14821 gtttgcacga gtccacttca atctgatttt taccctcgta acgcgatact attgccgtgc
    14881 taccaatgga ttggtgaacc tttaactcaa cccttacttg tgcatgacat gtaattgaaa
    14941 ttgccgctat taccgttgat gccaataccg ataaatgatt tttacccata ttttacgcta
    15001 tttaagcaga tactttgcca gtttactttt tagttcctga ggtttgaaag gtttaagcac
    15061 aaaatcgttc ataccgatgc caaaaatacg ttgctgcaca ttgagtaaag cagaggcgct
    15121 aagggcaatt attggaattg aggctttggc cttattgtgt aacttgcgga tggccttagc
    15181 tgcctcgtag ccgttcattt cgggcatttc aaggtccatt agaattaaat cgaaatcatt
    15241 ctgtgctaaa cagctcaagg cctcacgccc attgcttgca tgctcaaaat cgatacccca
    15301 gttggtaagg aaacgtttaa caatgaacgt gttgagttca ttatcctcca caatcagaac
    15361 ccgatgtttc ccgggtggaa tgttgatctc tactgtatca tcggtgggtt gaactgttcc
    15421 aactttgaac gggatggtaa aaaagaaact tgaccctttg ccttcgtcgc tttcaaggct
    15481 aatttcacca cccatcagtt ttacaattct ttgggagatg gctagaccta aaccagtgcc
    15541 ggtagctcta aacttaccct catgtacctg ggtaaactca ttaaaaatat caacctgcat
    15601 tttcttagga attccaatgc cggtatcgga tacagtaaac ctaattgtac tgatttcctt
    15661 gtttttttca acgagtttgg cggtgaagct aatttcgccc ttctcagtga attttatggc
    15721 attcccaata aggttggtaa gaacctgatt taaacgaacc cggtcgccaa tgagtggggt
    15781 tggaatattg gtgtcaatgt tataggtcag ggttaaaccc ttgctcgatg ccattttagc
    15841 aaaagtgctg tatagccctt taaacaaatc gttgatgtca aagttggatt gaagtaaatc
    15901 gagcttatgg gcttcaattt tactgtaatc tagtatgtcg ttcaggagcg aaagtagatt
    15961 ctcagctgcg tatttaaggg agtttaggtt gtcaacctgc tcgggttttg gatcctcatc
    16021 aattaaaagg tttgccatac ctattatggc gttcataggc gtacgaattt catgactcat
    16081 agttgaaagg aacttttcct tagcttggct tgccttttcg gcaagttctt tggctttacg
    16141 gagttgttcc tcgttcaggt gcgactcgta tgaggccgac aggatatttg acatggttaa
    16201 aaggaaatcc ctgtcgtcgg cggctttaac cttatcgccg taagggtagt caactgcaaa
    16261 aatgccttgg catttctctt ttacacgaat ggggaagagc agtaagtgtt ggctgctggt
    16321 taatttttta aggttgtcgt acgagggcat ggttgagccg gaaatgtcga aaattacttt
    16381 gttctcctgc tggaaatgtg aagtaatgct ggtgtagtta tcggagtagt atacccggag
    16441 tggttcagcc aaatcattag ccgtacttga acaacaagta aaaatatcaa aatcactttc
    16501 actaccaata aggataaagg cctttcgtaa accaatttcg cttacaataa ggtttaacga
    16561 ttcgtttgcc tgctttgaaa aagggaggtt tttgttaaaa acgtacgaaa cctttgtgag
    16621 cagtatttgc cggtcgaggt gtttctggat ttttatttgg ttaatttttt ggtcggttat
    16681 ttttcgtgct atcccagtaa tggcaattac ctttttgttc acattaaaca caggtgttag
    16741 ctgaaattcc caccattcct caattctgcg ttcgccttca cgaatctccc attttacagt
    16801 ttgcttgctt tttagcacct cgttttcctt ctgtttgaga gtatcgagaa gtggcgaggg
    16861 ccaaggttca gtatctacct tgcccaaaat ttgatcatcg gtgattccaa agtggttggt
    16921 gaactttttg ttcactagca aatagccaca tttgctatcc ttgagccagg caatgaaagg
    16981 taaattatca attgttcctc ttagctgtgc tctactacgg gcaagctctt cttcaacctg
    17041 tttacggtag gttatatcgc tcatggcaac aagcaagcaa tcggtgccct gtatgttgat
    17101 aatctcagcg gagagaagcg ttgtaaaggt ttcacccttt ttattaacta tctccacctc
    17161 aattttatcg accctgttct cccgctttac agcatcaatc agctcattgc gccgttcaat
    17221 ctgcttaaat attctaagtt ccgatgattt ttgcccaaca acttcagagt aatcgtatcc
    17281 tatcttttcc aaaaacgctc tgtttgcatc aatgtatgta ccctcgttaa gggtgctaat
    17341 ggtcatcatt acagcaccag aattgaatgc tttgaagaac ttttcttccg attgcttaat
    17401 ggccgcttcg gcctcaatgc gttcagtaat gtcacgtgca ataacaaata caacatcgcg
    17461 cttgcgccag tagcccttta tcattctaac ttcaagcggg agaggctttc cgtttttacg
    17521 cacaaactca gtgatgtata gtttgcggtc gccatcaaaa acttccataa gcgaggttat
    17581 ggctatacct ctttcactct cggcatgtag tttgacaaac gactcaagca gtatctcgtg
    17641 ttcatgatgc ccaagggcgc tcagaacggc gttgtttatt tgtacaatgt tatagttgtg
    17701 gttaacgata aaaactaaat cgccaaaggc gttcacgagc atgctcaggt tctgctcctg
    17761 gttggccatg ctctcctctg cttccttttg ttgggtaatg tcgtagctag tacataggag
    17821 tgccggcgat tcctgccaat caattattga aaaaatggtt ttaatccatt ttagctcgtt
    17881 atagcgggtt agaatacgca gggtaaagct gttggatgtg tgcttgcgga tttcggcaaa
    17941 atcgttgttg aacagtattt taaccagctt tctgtccttg gggtgaacca cgtcaaggaa
    18001 aagtatttcg ttgagttcat cctgtgcata gccggttagc ttagtaaaac ttggattggc
    18061 aaaaaccacc ctaaagttct ggattaggta tatgctgctc tcgttctgaa agattaaggg
    18121 tatcagaaag ttggaatcaa aattatccca attactgtag gagttattat tttggctatc
    18181 agagctcata cttaccgtgg caaaatcccg caaaagttac aaaaaaatgt ttacagtttg
    18241 ttgcttcgca taatttggcc tcttgttcat atcattaaaa ttattatttt agtgaaaaaa
    18301 ttggggttac gaattttctc tttttaagat ctttgctaaa acaaaataaa cctcaaaatg
    18361 taaaaaaaaa cgatgatatt gataatttaa ttgatttttg ttcaaatatt taatattgtg
    18421 ataatcaaaa agattgcttt ttacattgtg atagcaacta tatttttctt ccttacaaat
    18481 ggcagttgcg ataaaacaga atgtggagaa ttctggttta taaaccgtac tacacatagg
    18541 gtgaaaatta attcatatac atatttcggt ttaataactg atatgagtta tgaaatacag
    18601 caaattgatt ccatatcaaa agttacctgc gacatgggca tgccttcgaa tccttttaca
    18661 tcaaactatt ttcaggtaga ttctatagtt attgtgtttg atgatactct ttcaataaac
    18721 atatttaaat atttagaata caatcccatg aactttaaag ttcggaaaaa gaataaattt
    18781 gtttatgaaa ttaccgattc gctttattgc aaaactcttg aaataaacgg gtactcaagt
    18841 aaaattgttt ccctaccagt aaacggcagg gggactttaa ctcaatttga atagttcagt
    18901 gccaaaatta ttactaaaag ctctaagggg cttactaata aaaaaaaatg catacttttt
    18961 taaattttaa aagcgatgaa agcatataaa ttatacctat ttgctgtaat tgtatccttt
    19021 ctaaattctt gtggagataa ggatgtatgt gaagattaca ggtttgaaaa tgccacggat
    19081 catagggtat atatccggtc ctgctgcggg gaagcaactg acagcaagac atcccttagc
    19141 attggaccta agagtagcgc tacattaacc agttgtggtt taggttatgc tcaattcgta
    19201 tataatggag ttagtagatg tgcagtggat aaagtaataa ttatttttga cgatacgctg
    19261 gagttcaaca cgcaggagtt ttgggagttt gacctattaa atcctttaaa ttaccagcgc
    19321 aggaataagg agtacattta cgaaataaac cagttgcact accaaaaagc gcttgaggtg
    19381 aacgggtact taaagtaaaa cggtctccct gccagtaaaa ggcagggagc gtttagctaa
    19441 acttgaacag cgacaacaga tttgaaatga gtacgccgta caccccatcg gttctaaaac
    19501 caaagctcaa ggttgggtat taccaccacg accactggag ggatattaat ggcagtgctg
    19561 ttccattcag ggaaaaccta accatacccc atgtgtgcat ttatggtaag gatggctcag
    19621 gtagctggag taccactgat tttgtttatg caactacctg ccacgaggtg gtacatgtat
    19681 cgcactggga aatgattggt gaaggtgctt ttgccatttt atggcttaat ccaaaaacca
    19741 gaataatccc tgagagttgg gctgttgctg tagaatgggg tcttactaac ttggaatata
    19801 aagtattagg gcagaagtat ttgagtcctc aggctttgag ttataatttt aattacggct
    19861 atcaatcttg gtacttggga cggaatgatt tttatacacc actatttatt gatttgatag
    19921 atgattttaa tcaaaagacc tattataatg gggataaccg tcctgatgat aaggttaaaa
    19981 attacacttt atcttctata gaaagcattc tttttggtgt acgagattat gaagtactta
    20041 aagccatgct caaaatgaac aaacctgttg gggttacaga tggggatatt gacgatttga
    20101 ttaattatta tagaaatatt taaacctatg aaacctatga aaacgttttt caaattatta
    20161 atatcagttg ggttaatcgt gatattttca catgccagtt gtgataagtg ggtttgtgga
    20221 gataactggt tcgtaaataa aacaatgcat aagataaatg tgaatgtgta ttgtcatggt
    20281 gaagcgaata atgaattatc gtttcacatt gcgccttatg attcaataaa tagaatgact
    20341 tgtgggttgg gtgggcctgt cgacccgttt acttcaaata cctgtggccc tgattcaatt
    20401 ttagttgttt ttaatgatac tattccatta aatactttta ggtatttgga atacaacccc
    20461 atgaatctta aaaaaatcgg taaaactaag tttaggtatg agttcactga aatgcactac
    20521 caaaaagcgc ttgaggtgaa tgggtactta aggtaaaacg gtctccctgc cagtaaaagg
    20581 cagggagcgt ttagctaaac ttgaacagcg acaacagatt tgaaatgagt acgccgtaca
    20641 ccccatcggt tctaaaacca aagctcaagg ttgggtatta ccaccacgac cactggaggg
    20701 atattaatgg cagcgcaatt ccgttcaggg aaaacctaac cataccgcat gtttgtattt
    20761 atggtaaaga tggatcaggt tactggagta ctactgattt tatttatgca accacttgcc
    20821 acgaggtggc acacgtatcg cactgggaaa tggtaggcga gggagcattt gcccttatat
    20881 ggcttaatcc aaaaaccaga ataatcccgg agagttgggc agaagcagta tcgtggcaat
    20941 tgaccaggaa tgaatataaa cgatttggag attttgcgtt aagtagtgct gatttcaatt
    21001 ttagtaaaca agtttggagc aacaatatga acaaatatta tacaccaatt tttattgact
    21061 taatcgataa cattaaccag agagtaaaat actctggctt actgaactac cccaatgata
    21121 atgtaacagg ttacacagtt gctaagctgg aacagctact gtatgcattc atggatttag
    21181 acttgctgga ggcaacttta ctggtaaata aacccagtgg ggttaccaat gagagcatta
    21241 aagaactagg gtttctataa aaacctataa gtaccatgaa aaaaagtaaa ctctatttaa
    21301 ttgctgtgtt tgcatcactt ttttattcgt gcgataaaag tgtatgtgat gattacaggt
    21361 ttgaaaatgc cacggatcat agggtatata tccggtcctg ctgcg
//
LOCUS       DAOSAL010000043        21153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_1776_length_21153_cov_49.251398, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000043 DAOSAL010000000
VERSION     DAOSAL010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 21153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21153
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1776_length_21153_cov_49.251398"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(171..893)
                     /locus_tag="PK049_07230"
     CDS             complement(171..893)
                     /locus_tag="PK049_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOK61388.1"
                     /translation="MRAMILAAGLGTRLLPLTADKPKALIEVNGKTLLEICINNLIGF
                     GFNHIVVNVHHHAQQIIDFLRNSSFNAQIDISDETERLLDTGGALVHARSFLDQGEPF
                     LVHNVDIISNIDLHYLYQYHLNSSALASLAVSKREASRVFLFNEQMVLSGWRNMVTGK
                     VIITNPSKALTAYAFSGIHVISPEIFKQLPPKGEFSIVDAYLKLSKEFLIQGADLSHS
                     DVIDVGKPDTLELASQFVTRKV"
     gene            complement(900..1025)
                     /locus_tag="PK049_07235"
     CDS             complement(900..1025)
                     /locus_tag="PK049_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61389.1"
                     /translation="MSKKKCAKGKAGVKVEGKDYTCIKCGASSNSKDKLCKPIKK"
     gene            complement(1030..2457)
                     /locus_tag="PK049_07240"
     CDS             complement(1030..2457)
                     /locus_tag="PK049_07240"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOK61390.1"
                     /translation="MHSISHIENLSKLFSEVYGHSALNIVQLPGSGSARIYFRIESQQ
                     LSCIGTFNDDVKENETFYYLTEHLHHRNISVPKVLGVSSCRRYYLQSDLGSLSLYDKI
                     TDANSDTLEVQNLIGQAISNLARFNILGFDGIDESKLYPISHFDIKSVMWDLYYFKYC
                     FLKPSGVTYSEAELENVFGYLAEKVLNGDNQFLQFRDFQSRNIMILANQPYFIDYQSA
                     RVGSGLYDIASFLYQAKANFSDSTRQQFLVHYLDELSKYRSIDRSEMVAQFPYMAIFR
                     ILQTLGAYGFRGLFERKTHFIQSVPLALHNLSNLLAKVDDRRLRYLSQLNKELLSKYQ
                     VEQSEDFDGLTVQVYSFSIKSGYPPLNPEHGGGFVFDCRYLPNPGQLDLYKNLTGLDK
                     PVIDYLLSLDEVNVFCDNAFSLVKSAIDRYLSRNFKHLSVGFGCTGGQHRSVFCAENL
                     VKQLKSTFGDSGLRILVKHIELEKR"
     gene            complement(2450..3136)
                     /locus_tag="PK049_07245"
     CDS             complement(2450..3136)
                     /locus_tag="PK049_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HOK61391.1"
                     /translation="MTGTLVNVIAVLAGGSIGLLIGSRMPEKYSKVFFQVVGLFTLVL
                     GLSMAIKTNEPLLVVFSLILGVLSGTLLDLQSRLDNLSNRLKNRFKISGNRFSEGLVV
                     AFLLYCMGSLTVLGAIEEGLGGTPKLFYLKSLMDGFSSIVLASTLGVGVLFSALPLLL
                     YQGSLTLIASFAASSLSGSMVNELSAVGGILLVTLGFNILEIKRFHVINMLPALIFIV
                     ILTSLFANHA"
     gene            3257..3739
                     /locus_tag="PK049_07250"
     CDS             3257..3739
                     /locus_tag="PK049_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61392.1"
                     /translation="MPLPTSSKMRISLLKKDEIIDFVEFNITGDAENYIRCDDESYYL
                     QTNVFNIFAGCFERSHHLFDYISPTMYNARRFIPLRNELLVNLEKLLVIKNVSQFQQY
                     LSQVFLGKDLLEVLKQIDPEYERHWKGYIRRLIQVNRHIMRLIDQCIDEERVLWVIPY
                     "
     gene            complement(3749..4837)
                     /locus_tag="PK049_07255"
     CDS             complement(3749..4837)
                     /locus_tag="PK049_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase"
                     /protein_id="HOK61393.1"
                     /translation="MKLVADSHIPYLKGVFEPFFDVEYLPGHLINREVVKDADVLIIR
                     TRTICSQDLLQGSRVKFIASATIGYDHIDTQYCDSNGIRWVTAPGCNAAAVQQWVGAA
                     LVKWISSKHLNPKTLTIGIVGIGNVGSKVVALGNALGMNVVCCDPPRAERENIKSFVD
                     LHELLKVSDIITFHVPLTRADNYPTSHMLSSKNLALCKPTAYFLNSSRGGVVDEVALL
                     SYLEQNPNVAVALDVWENEPDLNLELAKRCLVATPHIAGYSLEGKVMATKMVVDAVSR
                     YFNLGLTPWWPTPNPLTVKQVIGSAENILDLIGKTYRIERDDIRIIAKDFEDYRNTYN
                     YRRDFTGFKVLKIEEDAEKVGMLGFELG"
     gene            4931..5380
                     /locus_tag="PK049_07260"
     CDS             4931..5380
                     /locus_tag="PK049_07260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HOK61394.1"
                     /translation="MIVAPLSEFGTYLNLHPLFERVEQALITLDFSKPGEKIYVDGEN
                     LIAIPSFNKARSAGEAPLESHDKYIDIQICLQGEETMGWRDRQTCSAIKEPYNSDNDI
                     TFYSDHPTCFITLKPNQFAIFFPSDCHAPLIGEGFIKKVVFKVKLAM"
     gene            5430..6725
                     /locus_tag="PK049_07265"
     CDS             5430..6725
                     /locus_tag="PK049_07265"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOK61395.1"
                     /translation="MKRNLLRIAAVPAMLTMGACATILNTTKQKVNLTSNPIGAEVYI
                     NGKSTNKTTPCAVWVKRKVKKGPANGRNQYIYEFRKDGFVPYTYTDNAEVSSKIWLNL
                     FLNVAALPAFGVDFISGGAYYYNNDVLATLQKESAGYVVIRDTVVKQVYVTSPEAKGY
                     MFEKLSDVDVDIPNNNLEYPLRFALIIGNEDYSSKQVDLNREVNVDFARNDASAFKEY
                     AIKVLGIPESNIIFLLDATAGQMNQAIAKTNLIIKNAKGKAEVFVYYAGHGLPDEVTR
                     EPYLIPVDISGQNARDGIKLADLYAKLTEYPTKRVIVFLDACFSGGARNKELVAARGV
                     KVRPTESVLQGNIASLSAVSGEQSALPFKDKHHGFFTYYLLKKLKETKGNITFKELST
                     YLDEIIPLQSVLLNSKEQTPVLRMGINPTEDLENWVLTK"
     gene            6733..9114
                     /locus_tag="PK049_07270"
     CDS             6733..9114
                     /locus_tag="PK049_07270"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOK61396.1"
                     /translation="MALYLLKLGAKPSFFYSMIKKIIGILFITFLCFQGIKGQVKYNV
                     SGYVKDSQTGEVLIGATVIVKGINIGTATNSYGFFTITVPKGEHTISFGYLGYKTQEM
                     TLQVNKDNRIDIEISPNAQEIQEVVVSREKGNSNVQRAEMSLVKLDMKSVKQIPALMG
                     EVDVIKAIQLLPGVQSTAEGGSGFSVRGGASDQNLIILDEATVYNASHLMGFFSVFNN
                     DAIKDIKLYKGDMPASFGGRLSSVLDIKMKEGNSKDFNITGGIGSISSRLTVEGPILK
                     DRSSFMVSGRRTYVDVFFPLFNNDDLKQSTLYFYDLNAKLNLTLNPNNRIFISGYFGR
                     DMFGRDINGFGFGNQTLTARWNHIFKHNLFMNTTLISSNYTYFLESNTNDANAIRWES
                     NMDEVSAKIDFNYAIGPKYQLRFGVSSAYLTFMPGLIKGTNSQSFIQRWEIPKQHTLE
                     HGIYLSNEFDLGRISFKAGLRISAFQNIGKGQSLVLSPDYKVTDRISHPKGEIYNTFI
                     SPEPRVGLVYRLSDSSSIKASYSHTTQYVQLASNSQGGNPLDVWFPASPNIKPQQADQ
                     WALGYFRNFLNNQIEASAEVYYKNVKNFVDFKDFADVLLNDELEADIRQGKAYSYGLE
                     LFTRIDLGKWEGWVSYTYGRTFRKTQGVNMGRVYSATYDKPHNTTVVLSYRLNKQITL
                     SANWIYSTGQTYTQPTGRYEIDNITIPIYSERNAKRYPDYHRLDFAVNIKTKKSLNRR
                     WKSEWNISVYNLYNRHNAWTINFVQDDKNPNITYAEKTYLFPIIPSVTYNFKF"
     gene            9117..9983
                     /locus_tag="PK049_07275"
     CDS             9117..9983
                     /locus_tag="PK049_07275"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HOK61397.1"
                     /translation="MRKISTIIGILTLALAACTERINIELDSSDSRLIVYGSITTDTM
                     SHSVRLIKSVPFYSPTPPPPVSNATVYIMEGNTRYDLTEDANNPGYYYTTPDVFGKSG
                     TTYTLHIEDVNIGGVTSFQAQTQMPTLLEGYQTNYLDSINVSYNRNWDGWVVNGYAKE
                     PQNMRNYYMFRVKINDILYSDSLSNLVLVDDKFFNGNNTEGAAFYFISEKDTLKVGDK
                     VTLELCAITQDYYQYLNEALKASSPQFPLFSPPPANPITNLNNGALGYFSAYAIIRAS
                     YTMKEEDFEGQP"
     gene            complement(10076..13183)
                     /locus_tag="PK049_07280"
     CDS             complement(10076..13183)
                     /locus_tag="PK049_07280"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOK61398.1"
                     /translation="MSESLLDALIKLFAILVNIESKADSGFALDVLEDYLHHDFSDEQ
                     AKVFIDRFTNFGVSYRKELEDVSLDRYTESPMVHAIIDSINHEFEQHQKVWLVLQLLE
                     FLADSRLTTPDRLEFARRVANQFNISDFEFSNGRDFILSESPGQIPWNQQVLLISPER
                     DNPIPEIKHLVNDRLQGQLYVLRIASTNTLLVKYFGELELFMNSRMLKPGRTYLFGPG
                     AVIRGPRMSPVYYSKVVSVFIQTPGKPFVSIIAKDIEYKHKGSRNGVYPFSFSAYSGQ
                     LVGIMGGSGVGKSTLINLLNGNLKPVSGKVYINGYDVHNSKTELEGVIGYVPQDDLLV
                     EELTVFQNLYYNALFCFSHLSKNEVVELVDKTLVDFDLVEARDLKVGNPLNKYISGGQ
                     RKRLNIAMELMREPSVLFVDEPTSGLSSLDAERIMLILRKQTFKGKLVITNIHQPSSD
                     VFKIFDKIIVLDQGGRPIFQGNPMDAVVYFKRMANFLKPEESECITCGNVNTDIILRI
                     VEARVVNEYGRLTRKRKRSAKEWYELYLQNIQSKIIVAEPACSSPIPPNNFKIPSKLQ
                     QMWIYFRRNLRSKLANSQFMNISILASPVLAVVISFFSKYIHGTLTNPNAYIFSENDN
                     IPAFIFMSVVAMIFLGLTISAEEIINDRKVLFREKFLNLSYFSYINSKLVVLSFFSAF
                     QSLLFVLISTYVLEIQGMFWSYWAILFSTSFCASMIGLNLSAALNSVVAIYVMIPLII
                     VPQLMFSGVVISYNKLHKSLSHSEYVPIIGDLTLSRWPYEALCVQQFSNNEYNKHFFF
                     INKDISNNNYNATLLIPKLVLLLQEAQTDLAVGKQTPRTRQRLSIVQNELERLKHNFP
                     IINFTYPDTGLLGISRVTPSYIDITLAKLDTLKSHFNSKYRVGVERRDALVKSLEKEL
                     VGKTTLFELSRMHHNNALEALVTNRNDFTKIVEVDNKLVRRYEPIYAYPTARNGRAHF
                     YSYYKILGTLLIPTFWFNLVVIWLMSVIFYLTLLSDMFRVVNRFVERFRFRKLTARIA
                     RYLPT"
     gene            complement(13376..14848)
                     /locus_tag="PK049_07285"
     CDS             complement(13376..14848)
                     /locus_tag="PK049_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 protein"
                     /protein_id="HOK61399.1"
                     /translation="MKAIQLFALTAFTYVVLSSCQKVGTIRVDSNAGISFAGKTYEVF
                     TTANDSVTRLALTSKGEFDSARQPFETEVAVFVNPSKQFQEIIGIGGAITDAAAETFA
                     RLTPENQQKLLDAYFHPVKGIGYSFIRTSIHSCDFSSGSYTYIADGDSALATFSIAHD
                     MQYRIPLIKKALETSGSTTKLLASPWSPPAFMKDNNNMLYGGKLMQRYYQSWANYFVR
                     FVQEYEKAGIPVWGVTVQNEPMARQTWESCIFTAEEERDFLKHYLGPTFWNNGLKDKK
                     IIVWDHNRDLMPYRADVIFSDPEASKYAWGIGFHWYEDWWGGKKMYDNVRNVHESYPE
                     KHIIFTEGTVERFVPDHYNYWPNAEKYATSMIYDFNSGTSGWIDWNILLDEQGGPNHA
                     GNFCFAPMHGDTRTGELFITPAYYYLGHFSKFIRPGARRISTSPSRSNLLAASFNNTD
                     GSIVTVVLNTTDLPINYYLICSNAMAQLQIPAHAIQSIRY"
     gene            complement(15152..>16277)
                     /locus_tag="PK049_07290"
     CDS             complement(15152..>16277)
                     /locus_tag="PK049_07290"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOK61400.1"
                     /translation="AQMAFDRVLQQVDSNNLMLKALRQKLEAERIATRIGLSPSNPQA
                     EVIYQWGSPIDIGNKTTISITQQFDFPTAYIYRAKLAKGMSSALENEYRSKRSEILQK
                     AGLLYADLVYHNALLVELNRQLKNALVIAQSLKQKFEAGEIGVIPYTQAELVYHELNS
                     RITQVKLEQKSLADELRALNGSVAIAIADSVIIVPELPDSFDSWFGLAEPQNPELAYL
                     KSEIENSRLKLKLSKAEVLPTFNAGYASEKVGGERFQGIVAGISIPLWEKSNTVRYAR
                     ANERYMQLLTDDYLFSFRQSSQRLYSKVKALQELTINLSQNISSFDIAGKLYLALQKG
                     EISIVDYYTELNSYNSIKERLLEVQHECLKTYIELTRFAY"
     assembly_gap    16278..16377
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(16478..19585)
                     /locus_tag="PK049_07295"
     CDS             complement(16478..19585)
                     /locus_tag="PK049_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOK61401.1"
                     /translation="MLDRIIGFSLRNKYLILISTLVLAVIGLRTVSNMDIDVFPDLTA
                     PTVVVMTDAHGMAAEEVERLVTFPIETAVNGATDVRRVRSVSAQGFSFVWVEFDWGVD
                     VLKARQVVSEKLIAVAQQMPLGVSQPMLAPQSSVMGEIFFIGLQADSTNMMELRTIAE
                     WTVKPLILATGGVSQVTIIGGDYKQYQVVANPTRMLHLGVSMHELANACRFISMNSNG
                     VNIREFGNEYVVRGIARTSDIEQLGQTLVATRNGMPVKISDIAEIRIAPATKMGYASS
                     NASPAIILSISKQPNINTLEVTRKIEANLENLKKSLPPDVKLNTKIFRQSDFIERSVG
                     NVQQALIEGAIFVTIILLLFLGSFRTTIISLLAIPISLLATFVVMKLLGININTMSLG
                     GMAIAIGSLVDDAIIDVENVYKRLRQNREKPEHERQSSFTVVYEASREIRASILNATL
                     IIIVAFVPLFFLSGMEGRMLKPLGISFIVSLFMSLLVAMTLTPVLARMLLTGENYLKQ
                     NENEKWLAAKLRNGYEKSLQWVLCRKRLVLGSTLILLVLSVILLFGLGRSFLPEFNEG
                     SLTLSVVTPPGTSLDETNKVGNYIEKMLLAVPEVTSTARRTGRGELDEHSQATNSSEI
                     DVNFQLKERSNDVFLADVRASLARIPGIAVTVGQPLGHRIDHMLTGTRASIAIKLFGD
                     DLTKLYTLANQIKTEIVGINGLVDVNVEQQVEVPQIQIRANRALLAEYGISLEEFNEF
                     VDIVLGGEKLADIYEGQQRFDLVVKLDSTYTYTIEGIKSILIDSPTHGKVPLYQVADV
                     ISTTGPNTISRENVQRKLVISANVAGRDLRGVVNDIRSRVDESITFPEGYRVEYGGQF
                     ESEARASRTLLIASLISILIIFMLLFVEFRNFSLAGIILLNLPLALIGGVFAIWLTSG
                     VLSIPAIIGFISLFGIATRNGILLISNYIRFRKMGFDVQEVVVMGSSDRLNAILMTAL
                     TAALALIPLAFKGHLSGNEIQSPMAKVILGGLLTSTFLNIYIIPIVYTMFDKNEIVSD
                     YDNQE"
     gene            complement(19598..21124)
                     /locus_tag="PK049_07300"
     CDS             complement(19598..21124)
                     /locus_tag="PK049_07300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOK61402.1"
                     /translation="MVKNYLIIALATFIFTACNDTQRQQSQNHEGHSHEQAEQAEEEV
                     AHFTAYTDSLEIYIVGNPIIAGKEVELTAHVTGMKSFKPVKVDSLMLNVTAEGKSYSF
                     TALNTGNDGIYRLKVQFDVNGKADASIEMVINGIKGFYRLGQFTIYHCEHDWADSPQH
                     SHASNTIKFTKEQARAVDFATEIVTQQPIGQVVKTTGMVMPAQVDEVVLVAGISGVVS
                     YTEKSVLPGMQVRKSEVLLSLISKGMADDNFALRYAEAKSRYELAKSAYERQKALAAE
                     QVISTAEFQKAKSDYEVALAAFKAFENGYSAEGQQIVSPANAEVLKVFVSNGDYVNAG
                     QPLVKLSKLSVSSIQCYVQPRFLNLLSSISDANIRVSSSGMVVNLKSSGGRLAAVGRT
                     ISSENHLIPVVLELPNVEVLPVGEVVDVYLLCQPKGNSLTVPVSALLEEQGNHFVMVQ
                     LTPETFIKREVKLGVTDGSRVEVLSGLQPGNRIVTRGAVYVKMAQSSGALDAHSGHVH
                     "
BASE COUNT         6424 a         4598 c         4248 g         5783 t
ORIGIN      
        1 cgcgctcccc tccgaaagcg ggtgcaaaag tagaaaagcg tttttaatta cgcaaatcaa
       61 ttttcgaact tttttaagaa aaatgacaaa aattcaggca acgttctgtt agacagtaag
      121 aaaaatttta aaaaaatttc atttgaaaaa gacgcaggga gtattgcagg ttaaaccttc
      181 ctagttacaa attgcgatgc aagttccaga gtatcaggct tccccacgtc aattacatcg
      241 gaatgggata aatcggcgcc ctgaatcaaa aattcctttg atagtttaag ataagcatca
      301 acaattgaga actcaccctt tggtggcagt tgcttgaata tttcgggtga aataacatga
      361 atgccagaaa atgcatatgc agtaagggct ttggaagggt tagtaataat aaccttacca
      421 gtaaccatgt tcctccaacc agatagtacc atttgctcat tgaaaaggaa taccctggaa
      481 gcctctcttt tacttacggc tagcgaggca agcgcagatg agttaagatg atactgatat
      541 aggtaatgta aatcgatgtt tgagattata tcgacattgt gaaccagaaa aggctcgccc
      601 tgatcgagaa aactacgggc atgaacaaga gcaccaccgg tgtcgagtaa tctttcagtt
      661 tcatcggaaa tatcgatttg agcattaaaa ctgctattac gtaaaaaatc aataatctgc
      721 tgtgcgtggt gatgaacatt aaccactatg tgattaaaac cgaaaccaat taggttatta
      781 atgcatattt caagcagggt ttttccgtta acctcaataa gggctttggg tttatcggct
      841 gttaagggaa gtaatcgggt gcctaggcca gcggccagaa tcattgctct catataaact
      901 tactttttaa taggtttaca aagtttatcc ttgctatttg agctagcacc acactttatg
      961 caggtgtagt ccttaccctc aaccttaacc cctgctttac ctttcgcaca tttctttttt
     1021 gacattctgc taacgctttt caagttcgat atgcttaacc aaaattctta atccactatc
     1081 gccaaaagta cttttaagtt gcttaacaag gttctctgca caaaatactg atcgatgttg
     1141 gccaccagta caaccaaaac caaccgataa atgtttaaag tttcgagaca aatacctatc
     1201 tattgccgac ttaactaatg aaaaagcgtt atcgcaaaaa acatttactt catccaaaga
     1261 aagtaaatag tctattacag gcttatcgag tccggtcaga ttcttgtaca aatcgagttg
     1321 gccaggattg ggtaaatatc ggcaatcaaa tacaaaccct ccaccatgct caggatttag
     1381 tggtggatag ccactcttaa tagaaaaact atatacttga acggtaagac catcaaaatc
     1441 ttcactttgc tcaacttgat atttacttaa taactctttg ttaagctgac tgagatatct
     1501 taaacggcga tcgtcaacct tagcaagcaa attgctgagg ttatgcaacg caagaggaac
     1561 gctttgaatg aaatgggttt tacgctcaaa aagacctctg aaaccataag ccccaagtgt
     1621 ttgcaaaatt ctaaagatag ccatataagg aaattgggcc accatttcac tcctgtcaat
     1681 tgaacgatat ttggacagct catctaggta atgcactaga aactgctgcc gagtagaatc
     1741 gctgaaatta gcctttgcct ggtatagaaa agaagctata tcgtaaaggc ctgagccaac
     1801 tctggcgctc tgataatcaa tgaaatatgg ctggttagcc agaatcataa tattacggga
     1861 ttgaaaatcg cggaattgca ggaattgatt atcgccattg agcacctttt ctgctaaata
     1921 gccaaagaca ttttccaact cggcctcact ataagtaacc cctgaaggct taaggaagca
     1981 gtacttgaaa tagtacaaat cccacatcac cgatttgata tcgaaatgcg aaattgggta
     2041 aagctttgat tcatcaatac catcaaaacc caaaatattg aaccgggcaa ggttggatat
     2101 tgcttgccca atcaaattct gaacttcaag agtatcgctg ttagcatcag ttattttatc
     2161 gtacaacgac aatgaaccca aatcggattg taagtagtac cttctacaac ttgaaacacc
     2221 caataccttt ggaaccgaaa tattgcgatg gtgtagatgt tccgtgaggt agtaaaaggt
     2281 ttcgttttcc tttacatcat cgttaaatgt tccaatgcac gacagttgct gtgattcaat
     2341 cctaaagtag atgcgtgcag aaccagaacc gggcagctga acaatgttaa gggccgaatg
     2401 cccatatacc tcggaaaaga gcttggaaag attctcgata tgggaaatgc tatgcatggt
     2461 ttgcgaaaag tgaagttaga ataacgatga atattagtgc agggagcata ttgatcacgt
     2521 ggaaacgttt tatctctagt atattaaacc ctaaagtcac taaaagaata ccgcctacag
     2581 ccgatagttc atttaccatg cttccactta ggctgctagc cgcaaaagag gcgatcaatg
     2641 tcaatgaacc ctgatatagc agcaagggta aggcggaaaa cagaacccct accccaaggg
     2701 ttgatgctag cacaatggat gagaacccat ccataaggct tttgaggtaa aaaagtttgg
     2761 gtgttccacc caaaccctcc tcaatggcac ccagaacagt taaagaaccc atgcagtaaa
     2821 gcaggaaagc cacaaccagt ccttccgaaa agcgattccc tgatatttta aatcgatttt
     2881 ttaaccggtt cgataggtta tcgagcctgc tttgcaaatc gagtaaagtc cccgatagca
     2941 cacccagaat taagctgaat acaaccagta agggttcatt ggttttaatt gccattgata
     3001 agcccagtac cagcgtaaag agcccaacca cttgaaaaaa aactttactg tacttttcgg
     3061 gcatacggga tccaataagc aaacctattg agcccccagc caatacagct attacgttaa
     3121 ctaaagtccc agtcaccttt tagcattttt aatatactgc taaaatagaa tgtttacgtc
     3181 agaataaaca attgttaaaa aaaaatttaa gagtaaatga ataaagttag tatatttgaa
     3241 ttgaaaaata tgatttatgc ccttacccac tagtagtaaa atgcgtatta gcctgttgaa
     3301 aaaggacgaa atcattgatt tcgtggagtt taatattaca ggcgacgctg aaaactatat
     3361 ccgttgcgat gatgagtcgt attaccttca aacaaacgtg ttcaatatct ttgcaggatg
     3421 ctttgaacgg agccatcatt tattcgatta catctcgcca accatgtata acgcccgacg
     3481 tttcattcct ctccgtaatg aacttcttgt aaaccttgaa aaacttttgg ttattaagaa
     3541 tgtatcgcaa tttcagcagt acttaagcca agttttttta gggaaggatt tgctggaggt
     3601 tttaaaacaa attgatcctg aatacgaaag gcactggaag ggatatataa gaaggctcat
     3661 tcaggttaac cggcacatca tgaggctcat tgatcaatgc attgacgaag agcgtgttct
     3721 gtgggtaatt ccttactaag ccgattggtt aacccaactc aaacccaagc ataccaacct
     3781 tttcagcatc ttcttcaatc tttaatactt tgaaaccggt aaaatcgcgc cggtagttat
     3841 atgtattcct gtaatcctca aaatctttag ctataattct aatatcatct ctctcgatac
     3901 gatacgtttt gcctattaaa tctaaaatat tttcggctga gccgataact tgctttacag
     3961 taagtggatt tggcgtaggc caccaagggg ttaggccaag gttaaagtaa cggctaacag
     4021 catcaactac cattttagtt gccataacct taccctccag cgagtaacca gcaatatgcg
     4081 gtgttgccac aaggcatcgc ttagcaagtt ccaaattcaa atcaggttca ttctcccaca
     4141 catctaaagc aacagctaca ttagggtttt gctccaaata gctaagcagc gccacctcgt
     4201 caactacccc accccgcgat gaatttaaaa agtatgctgt tggcttgcac aaagccaaat
     4261 tcttactgct taacatatgg ctggtagggt aattatctgc tcgcgttaaa ggaacatgaa
     4321 aggtgataat atcggacacc tttagtaatt catgtagatc aacaaaagac tttatattct
     4381 ccctctcagc cctaggcgga tcgcagcaaa ccacattcat gcctaaggca ttgccaagag
     4441 caaccacttt actgcccaca tttccaatgc ctactatacc aatggttagg gttttgggat
     4501 tgagatgttt tgaactgatc catttcacaa gtgcagcacc cacccactgc tggacagcag
     4561 cagcgttgca accgggagcg gttacccacc taataccatt tgaatcgcag tactgagtat
     4621 cgatatgatc atagccaata gttgccgaag caatgaactt aacccttgaa ccttgcagta
     4681 aatcttggct acaaatagtg cgtgttcgaa taattagcac atcggcatct tttaccacct
     4741 ctcggtttat aagatgcccg ggtaaatact caacatcaaa aaagggttca aaaacacctt
     4801 taaggtaggg gatgtgacta tcggcaacta acttcatagg taaattttgc acaaagatag
     4861 caagtaaaaa ctgttatacc cagcattgtc aaactgtcaa aataaactaa ttttacaaaa
     4921 aatatgcgta atgattgtag caccattatc ggaatttggt acctacctaa accttcatcc
     4981 cctttttgag cgggttgaac aggcgctaat tactttggat ttcagcaaac caggagaaaa
     5041 aatttacgtc gacggggaaa acctcatagc catcccctcg tttaataaag cacgcagtgc
     5101 aggagaagcc ccattagaat cgcacgataa gtatatcgat attcaaatat gcctgcaggg
     5161 cgaagaaaca atgggctggc gcgacaggca aacctgtagt gctattaagg agccctataa
     5221 tagcgataac gacataacct tttactccga ccaccctaca tgttttatca ctctaaaacc
     5281 taatcaattt gcaatatttt tcccatccga ttgccatgca ccgttgatag gagagggttt
     5341 tataaaaaaa gtggtgttta aggttaaatt ggccatgtaa ttgcataaaa tttgtagtaa
     5401 aaatattacc aaaccaaact gaccaatcta tgaaacgcaa tttattgaga attgcagctg
     5461 taccggcaat gctaaccatg ggtgcctgtg caaccatact taacacaact aagcaaaagg
     5521 taaacttaac ctcaaatcca attggagccg aagtttatat taatggtaaa agcaccaata
     5581 aaactacacc ctgtgcggtt tgggttaagc gcaaggtaaa gaaagggcct gcaaacgggc
     5641 gcaaccagta tatttacgag ttccgtaagg atgggtttgt tccttacacc tataccgata
     5701 acgcagaggt tagcagtaaa atatggctaa acctgtttct aaacgtagca gccctacctg
     5761 cttttggcgt cgattttatc tcaggtggtg catactacta caataacgat gttttggcaa
     5821 ccctgcaaaa ggagtccgcc ggttatgtgg taatcaggga cacagttgtt aaacaggttt
     5881 acgtaacttc ccctgaagcc aagggttata tgtttgaaaa attatcggat gttgatgttg
     5941 atatcccaaa caataacctg gagtatccct tgcgctttgc gctgataatt ggaaacgagg
     6001 actacagtag caaacaggtt gatttgaata gagaggttaa tgttgatttt gcccgaaacg
     6061 atgccagcgc atttaaggaa tatgccataa aggtattagg gattcccgaa agcaacataa
     6121 tctttctgct cgatgccact gctgggcaaa tgaatcaggc cattgcaaaa acaaatctga
     6181 taataaaaaa tgccaagggc aaagccgagg tttttgttta ctatgcaggg catggcttac
     6241 ccgatgaggt tacccgcgaa ccatacctta taccggttga cattagcgga caaaatgccc
     6301 gcgatggaat taaactagcc gacttatatg ccaagcttac agaatacccc acaaaaaggg
     6361 tgatcgtatt tctggatgca tgcttctcgg gcggagcccg caataaggag ttagttgcag
     6421 cccgcggggt taaagttcgc cccactgaaa gcgtgcttca gggcaatatt gcttcactat
     6481 cggccgtatc gggcgaacaa agcgctctgc cattcaagga taagcaccat ggtttcttta
     6541 cctattattt actcaaaaaa ctaaaagaga ctaagggcaa cattaccttt aaggaactta
     6601 gcacctacct cgatgagata atacccctac agtcagtgct actgaacagt aaggagcaaa
     6661 cccctgtgct tagaatgggt attaacccaa ccgaggattt agaaaattgg gttcttacaa
     6721 aatagaacta atatggcatt gtatttgtta aagctaggtg ctaaacctag ctttttttat
     6781 tccatgatta aaaagattat tggcatactt tttattacat tcctttgctt ccaaggcata
     6841 aagggacagg taaagtataa cgtaagcggt tacgttaagg attcccaaac gggcgaggtt
     6901 ctcattggag caacagtaat agtaaaggga atcaatatag gcactgcaac aaatagttac
     6961 gggttcttta caattacagt tccaaaaggt gagcatacaa tctcatttgg atatctggga
     7021 tataaaaccc aagaaatgac tttacaagtc aataaggata ataggattga tattgagata
     7081 tcgcccaacg cacaggaaat ccaggaggtg gttgttagcc gggagaaagg gaatagcaat
     7141 gttcaacgag ccgaaatgag ccttgtaaaa cttgacatga aaagtgtaaa acaaatacct
     7201 gccttaatgg gtgaggttga tgtaattaaa gctattcaat tactgccggg ggtccaaagt
     7261 actgccgaag ggggatcagg atttagcgtt cggggtggtg cttcggatca gaacctgata
     7321 atactcgatg aggcaaccgt ttacaatgct tcgcatctaa tggggttttt ctccgttttc
     7381 aataacgacg caattaagga tatcaagctc tataagggcg acatgccagc ctcgtttgga
     7441 ggcagattat cgtcagtgct cgatataaag atgaaggaag gcaacagcaa ggattttaac
     7501 ataaccggag ggataggtag catatcgagc cggttaacgg ttgaaggtcc aatactaaag
     7561 gataggagtt cgttcatggt atcgggacgc agaacctatg ttgatgtgtt ttttccacta
     7621 tttaataatg acgatttgaa acaaagcaca ctctactttt acgacctgaa cgcaaaactg
     7681 aacttaactc ttaatcccaa caaccgaatt tttatatccg gatactttgg acgcgacatg
     7741 tttggccgcg atatcaacgg ctttggcttt ggtaaccaaa cgcttacggc aaggtggaac
     7801 catattttca agcataacct tttcatgaat actactttga tatcaagtaa ttacacatac
     7861 tttttggaat cgaataccaa cgatgcgaac gccattaggt gggaatcgaa catggatgag
     7921 gttagcgcaa aaattgattt taactacgca attggtccaa aatatcagtt aaggtttggc
     7981 gtatcgagcg catacctaac atttatgcct ggtttgataa aaggtactaa ctcccaatcg
     8041 tttattcagc gttgggaaat ccccaaacag catacgctgg agcatggtat atacttatcg
     8101 aatgagtttg atctaggaag aatttcattt aaagcaggtt tacgcatatc ggcctttcag
     8161 aacattggga aaggccaaag ccttgtcctt tcgcccgact acaaggttac cgacagaata
     8221 tcccatccaa agggtgagat ttataataca tttattagtc ctgagccccg agtgggctta
     8281 gtttataggc ttagcgatag cagctcaata aaagccagct actcgcatac cacgcagtat
     8341 gttcagctgg catcaaactc acaaggtgga aatccgcttg atgtttggtt tccggcatcg
     8401 cccaatatta agccccagca agctgaccag tgggcccttg gctactttag gaactttttg
     8461 aacaaccaaa tagaggcatc ggcggaggtt tactataaaa acgtaaaaaa ttttgtggac
     8521 tttaaggatt ttgccgatgt tctgctcaac gatgagcttg aagcagacat caggcagggc
     8581 aaagcatact cttacggatt agaactattc acacgtatcg acctagggaa gtgggaagga
     8641 tgggttagct atacttacgg cagaaccttt cgcaaaacac agggagttaa tatgggaagg
     8701 gtttatagcg caacctacga taagccccat aatacaaccg ttgtgcttag ctatcgactt
     8761 aataagcaaa ttaccctttc agccaactgg atttactcca cgggtcaaac ctacacccag
     8821 ccaaccggac gatacgaaat tgataatatt accataccca tatactctga aaggaatgcc
     8881 aaacgctatc cggattacca caggctcgac tttgccgtaa acattaaaac taagaaaagt
     8941 cttaaccggc gatggaaaag cgaatggaat atctctgttt acaaccttta caacagacat
     9001 aatgcatgga ctataaattt tgttcaggac gataaaaatc cgaacataac ctatgccgaa
     9061 aaaacatacc tattcccaat tataccctca gtaacctata acttcaagtt ttagctatga
     9121 gaaaaatatc gaccattata gggattttaa ccttagcact agctgcatgc acggaacgga
     9181 taaacattga actagattca tcggattcca ggcttatagt ttacggcagt ataaccaccg
     9241 ataccatgag ccactccgta aggctaatta aatcggtacc gttctatagt cctactccac
     9301 ccccacccgt ttccaatgcc acggtttaca tcatggaagg caatacccgc tacgacctta
     9361 ccgaggatgc caataaccca ggctactact acacaacccc ggatgttttt ggtaaatcgg
     9421 ggacaacata cacgcttcat attgaagatg tgaatattgg gggtgttact tcgtttcagg
     9481 ctcaaacaca aatgccaaca ctgctcgagg gatatcaaac caattacctg gattcaataa
     9541 acgtaagcta caacaggaac tgggatggat gggtagttaa tggttatgct aaagagcccc
     9601 aaaacatgag aaactattac atgttccggg taaaaataaa cgacatacta tacagcgaca
     9661 gcctcagtaa tctggttctg gttgatgata agttctttaa cggcaataac actgagggtg
     9721 ctgccttcta ctttatttct gagaaagata ctctcaaagt aggcgataaa gtaaccctag
     9781 agctttgcgc cataacccag gattactacc agtacctaaa cgaagccctg aaggcctcaa
     9841 gcccacaatt cccattattt agccctcctc cagccaatcc gattactaat ctaaataatg
     9901 gagcgttggg atattttagc gcttacgcaa ttattcgggc ttcgtacacc atgaaagaag
     9961 aggattttga gggacaacca tgaagattta cagataatat atgttcctaa tattttaaat
    10021 cacttcaaga tacagctcaa aactcaatta aataccagca ttgcgaaaaa caatgttagg
    10081 ttggcaaata ccttgcaata cgagccgtaa gcttgcggaa acggaagcgc tcaacaaaac
    10141 ggttaaccac gcggaacatg tcgcttagta atgtaaggta aaatattact gacattagcc
    10201 agataaccac cagattaaac cagaaggtag ggattagcaa agtgcctaga attttatagt
    10261 aggaataaaa atgtgctctt ccatttcggg ctgttgggta tgcgtatatg ggttcgtagc
    10321 ggcgaacaag cttgttatct acttcaacaa tcttggtaaa atcgtttcta ttagttacca
    10381 aagcctccaa agcattattg tgatgcatgc ggcttaactc aaaaagtgtg gttttaccaa
    10441 ccaattcctt ttcaagcgat tttaccagcg catcgcgccg ttcaacccca actctatact
    10501 ttgagttgaa gtgagatttt agggtatcga gcttagcaag tgttatgtcg atatagctag
    10561 gcgtaactct actaatgccc aggagtcctg tgtcagggta agtaaaatta ataattggga
    10621 aattgtgctt taacctttcg agttcattct gcacaatgct taacctctgc ctggttcgag
    10681 gcgtttgctt gccaacagcc aaatcggttt gtgcttcttg cagcaataac accagcttag
    10741 gaatcagcaa cgtggcattg tagttgttat tgctgatatc tttattgata aaaaagaaat
    10801 gtttattata ctcattgtta ctaaactgct gtacacaaag agcctcgtag ggccaacggc
    10861 tcagcgttaa atctccaata attggaacat actccgaatg ggaaaggctc ttgtgtaact
    10921 tgttatagct gattaccaca ccgctgaaca tcagctgcgg aacaatgatt aagggaatca
    10981 ttacgtaaat tgctacaacc gagtttaatg cggctgaaag gttcagacct atcatgcttg
    11041 cacaaaatga tgtggagaac agaatggccc agtaggacca gaacattcct tgaatctcaa
    11101 gcacataggt tgatattaga acaaatagta acgattgaaa tgctgaaaaa aatgaaagta
    11161 ctacaagttt tgagttgata tagctgaaat agctgaggtt aagaaacttt tcccggaaaa
    11221 gaacttttcg gtcgttaatt atctcctcag cactaatagt cagacctaaa aaaatcatgg
    11281 ccaccacgct catgaaaatg aacgcgggaa tattatcgtt ttcgctaaaa atgtaagcat
    11341 ttgggttagt aagggttcca tgaatatact tggagaagaa actgataaca accgctaata
    11401 ctggcgaagc cagaatgcta atgttcatga actgggagtt agcaagctta cttcgtaaat
    11461 ttcttctaaa gtaaatccac atctgctgta gtttcgatgg gattttaaag ttattgggcg
    11521 gtattggcga agagcaggct ggttcagcaa caataatctt actttggata ttttgtaggt
    11581 aaagctcata ccattcctta gctgaacgtt ttcgctttcg ggttaagcgg ccatactcgt
    11641 taacaacccg ggcttcaaca atcctaagaa taatgtcggt attaacattt ccacaggtaa
    11701 tacactcact ctcctcgggc ttaagaaaat tggccatacg cttgaaataa accacggcat
    11761 ccattggatt accctggaat attggcctcc ccccctggtc aagcacaatt atcttatcga
    11821 atatcttaaa gacatcggat gatggctgat ggatattggt aattacaagt ttccccttaa
    11881 atgtttgctt tcgtaaaatt agcattatgc gctcggcatc gagcgatgag agccctgagg
    11941 taggctcatc gacaaataga accgatggct cacgcattag ctccatggca atgttaaggc
    12001 gcttgcgctg ccctccgcta atgtacttat tgagcgggtt gccaacctta aggtcgcgtg
    12061 cctcaaccag gtcgaaatct acgagggttt tatctaccag ctctacaact tcatttttac
    12121 tgagatggct gaaacagaaa agggcgttgt agtagagatt ttgaaagacc gtaagctctt
    12181 caaccagtaa atcgtcctgg ggaacatagc ctataacccc ttcaagttcg gttttgctat
    12241 tatgaacatc gtacccgttg atataaacct tacctgaaac gggctttaaa ttaccattga
    12301 gcaggttgat aagggttgac ttgcccacgc cacttccgcc cataattccc accagctgac
    12361 cggagtaggc tgagaatgaa aaggggtaaa ctccattccg actccccttg tgcttatact
    12421 caatatcctt agctataatg gaaacgaaag gcttaccggg ggtttgaata aaaaccgata
    12481 caaccttgct gtagtatact ggcgacatac gtggcccacg aattaccgca ccaggaccga
    12541 aaaggtatgt tctccccggc tttagcattc ggctattcat gaaaagttcc aactcaccaa
    12601 agtactttac cagcaaggtg ttggtgctgg caatcctaag tacgtaaagt tgcccctgga
    12661 gtcggtcgtt aaccagatgt ttaatttccg gtatagggtt atcgcgttca gggcttatga
    12721 gaagaacctg ttgattccat ggtatttgac cgggcgactc gcttagtata aaatcgcggc
    12781 cgttggaaaa ctcaaaatca ctaatattga actggttggc taccctacgg gcaaactcaa
    12841 gcctatcggg tgtggtaagc ctgctatcgg ccaggaattc caacaactgt aaaaccagcc
    12901 aaaccttttg atgctgctca aactcatggt tgatagaatc aattatagca tgaaccatgg
    12961 gcgactcggt gtagcggtca aggcttacat cctctaactc cttacggtag cttaccccaa
    13021 agttggtaaa tctatcaata aacaccttag cctgctcatc gctaaagtcg tgatgcagat
    13081 aatcttccaa tacatcgaga gcaaagccac tgtcggcttt actctcaatg ttcacgagta
    13141 tggcaaaaag ctttataagt gcatcgagca gcgactcgct catggtttaa ggtttggata
    13201 agcaaagata gaatttttca attgtattga accgtacggt tataaattgg attgtaattt
    13261 tataatcgct ttacccaaaa aattggtgac aaccaccttt ccctagcgag tgcttttaaa
    13321 aatcatttaa ccttcatata attaattcaa tacccaacgc attactctac catatttaat
    13381 acctgatact ttgaattgcg tgtgctggta tttgcaattg agccatagcg ttactgcaaa
    13441 tcagatagta gtttatgggt aaatcggtgg tgtttagtac aaccgttact atacttccgt
    13501 cggtattatt gaacgatgcg gcaagtaaat tgctgcgcga tggtgaggtg cttattcgcc
    13561 gtgctccagg cctaataaac ttggaaaaat ggccaaggta gtagtatgca ggggtaataa
    13621 aaagctcacc tgttcgtgtg tcgccatgca tgggagcaaa gcaaaaattt ccagcatgat
    13681 tgggtccacc ctgctcgtcg agcaggatat tccaatcaat ccatcctgat gtgccactat
    13741 taaaatcgta aatcattgat gtagcatact tttcggcatt tggccagtaa ttgtaatggt
    13801 caggcacaaa acgttcaacc gttccctcag tgaaaatgat atgtttttcg gggtaacttt
    13861 cgtgaacatt ccttacgtta tcgtacatct ttttcccgcc ccaccaatcc tcataccagt
    13921 gaaagccaat accccaagca tatttcgatg cttcagggtc agaaaatata acatcggcac
    13981 ggtaaggcat caaatcgcgg ttgtgatccc aaacaattat ttttttatcc tttagcccat
    14041 tgttccagaa ggtggggcca aggtagtgct tcaggaaatc gcgctcctct tcggcagtaa
    14101 atatgcacga ctcccacgtt tgacgagcca ttggctcatt ctgaacagta acaccccaaa
    14161 ctgggattcc ggccttctcg tattcctgta cgaaccggac aaaataatta gcccacgatt
    14221 ggtagtaacg ctgcataagc ttaccaccgt aaagcatatt attgttgtcc ttcatgaaag
    14281 ccggtgggct ccaaggactt gcaaggagtt tagtggtact gcccgatgtt tccagcgctt
    14341 ttttaatgag aggtatcctg tactgcatgt cgtgcgctat gctaaaggta gccagtgcgc
    14401 tatcgccatc ggcaatgtaa gtgtagctac cgctgctgaa atcgcagcta tgaatactgg
    14461 tgcgaataaa tgagtaacct atccccttta ccgggtgaaa gtaagcatcg agtagcttct
    14521 gctgattctc aggtgttaaa cgggcaaagg tttcggcagc agcatcggta atggctccac
    14581 caataccaat tatctcctga aactgtttac ttggatttac aaaaacagcc acttcggttt
    14641 caaaaggttg acgagccgaa tcgaattccc cttttgatgt tagtgctagt cgagtaacag
    14701 aatcgtttgc tgttgtgaaa acctcatagg tctttccggc aaatgatatg cctgcattgc
    14761 tatcaaccct aatcgttcct accttttggc aagatgataa tacaacataa gtaaacgcgg
    14821 taagggcaaa caattgtatc gctttcattc caattccttt tttgagttcg tttaacaaag
    14881 atacataggt ggtgttaaga cttattggaa tgattcataa taatttttaa tgagcaagat
    14941 ttgagaaaat attgcaaaaa aagatttgct tataccatca atacttacca accatttgta
    15001 ggcttaccag cctcagtatt aaatgtgatt tgtgtagatt tgagaagcta atagttaact
    15061 tctactacat catcctcggg ttaatgaaaa aaacgcgcat caaataatgc gcgctcttta
    15121 actataaaaa aatccatgca aatacgaaac atcaataggc aaacctagta agttctatgt
    15181 aagttttaag gcactcatgt tgcacttcaa gcaatcgttc cttgatactg ttgtagctat
    15241 ttagctctgt gtagtaatca acaattgata tttcaccctt ttgaagcgca aggtataact
    15301 ttccagcaat atcgaaacta ctgatgtttt ggctaaggtt aattgtgagt tcttgcaatg
    15361 ctttaacctt actgtaaagt cgttgtgagc tttgcctaaa tgagaatagg taatcgtcag
    15421 taagcaattg catatacctc tcattagccc ttgcatacct cactgtattt gatttttccc
    15481 ataatgggat ggaaatacct gcaacaatac cctgaaagcg ttcacctcca accttttcgc
    15541 tggcataacc agcgttaaaa gtaggcaaaa cctcggcctt tgaaagttta agttttaaac
    15601 gactattttc aatttcgctt tttaggtagg caagctccgg attttgcggt tcagcaagtc
    15661 caaaccagga atcaaaacta tcgggcaatt ccggaacaat aattactgaa tcagcaatag
    15721 ctatagcaac cgaaccattc aaagctctaa gctcatcggc taagcttttc tgctcaagtt
    15781 ttacttgagt gattcttgaa tttaactcat ggtaaacaag ctctgcctgt gtgtatggaa
    15841 ttacacctat ttcaccagcc tcaaactttt gcttaagcga ttgggcaata accaaagcat
    15901 ttttcagctg cctatttagc tcaactaaaa gagcgttgtg gtaaaccaaa tcggcataaa
    15961 gcaaaccagc tttttgaagt atctcagagc gttttgacct atactcattc tctaaagcac
    16021 ttgacatacc tttggctagc ttagccctgt aaatatatgc agttggaaaa tcgaactgtt
    16081 gagtaatact gattgtggtt ttgtttccaa tatcgatagg gctaccccac tggtaaataa
    16141 cctcggcttg ggggttgctg ggcgataacc ctatacgggt agcaatcctt tcggcttcaa
    16201 gcttttgcct tagtgctttt agcataaggt tgttagaatc aacctgctgc aaaaccctat
    16261 caaatgccat ttgggcannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncat
    16381 aaggttgtta gaatcaacct gctgcaaaac cctatcaaat gccatttggg catttgtaat
    16441 gccagcaaaa gcaagcgcaa caaaaactaa gctaacttta ttcctggtta tcataatcac
    16501 ttacaatttc gtttttatca aacatggtgt aaacaattgg tataatgtat atgttcagaa
    16561 atgttgatgt taaaagtcca cccagaataa ccttagccat tggactttgt atctcgtttc
    16621 cggaaaggtg tcccttaaag gcaagtggta ttaaagcaag ggcagctgta agcgcagtca
    16681 taagaattgc gttaagccgg tccgacgaac ccataactac cacctcctga acatcaaatc
    16741 ccatttttct aaaacgaatg tagttagata ttaacagtat tccatttcgt gtagcaatac
    16801 caaaaagcga gataaatccg ataattgccg gaatgcttaa aactcccgaa gtaagccata
    16861 tggcaaaaac cccaccaata agcgccaagg gaaggttcaa tagtattatt ccagccaagc
    16921 tgaagttacg aaattccacg aaaaggagca taaaaattat taaaattgaa atgagagaag
    16981 ctataaggag tgtccgcgat gcacgggcct cgctctcgaa ttgtccacca tactcaactc
    17041 tatatccttc agggaaagtg atactctcat caaccctgct acgaatatcg ttaacaacac
    17101 ctcgcaggtc gcgccctgct acgttggccg atataaccag cttacgttga acattttcac
    17161 gactaattgt gttggggccg gtagttgaaa tcacgtcggc aacctggtaa agaggaacct
    17221 taccatgcgt aggcgagtca ataagaatcg atttaatccc ttcaatggta tatgtgtagg
    17281 ttgaatcaag cttaaccact aaatcgaaac gttgctgccc ctcgtaaata tcggcaagtt
    17341 tttcgccccc aagcacaata tcaacaaact cattgaactc ctcaaggctg atgccgtatt
    17401 ctgccagaag tgcccggttg gctctaatct ggatttgtgg cacctcaacc tgctgctcaa
    17461 cgtttacatc aaccaaaccg ttaattccca ctatttcagt ttttatctgg ttggctagag
    17521 tgtaaagttt agtcaggtcg tcgccaaaga gcttaatggc aatgcttgcc cgcgtaccag
    17581 taagcatatg gtcgatacgg tgccctaaag gctgcccaac agtaactgcg atgcccggaa
    17641 tgcgggcaag cgatgctcga acatcggcga ggaacacatc gttgctgcgc tccttaagct
    17701 gaaagttcac gtctatctcc gagctgttgg tagcctgcga atgctcatcg agttcgcctc
    17761 tgcctgttcg gcgggctgtt gatgttacct cgggtactgc cagaagcatt ttttctatgt
    17821 agtttcccac cttattggtt tcatcgagcg aggtgcccgg tggtgttacc accgatagcg
    17881 ttagcgagcc ctcattaaac tccggaagga agctacgccc caatccaaat aagagtataa
    17941 ctgacaaaac aagcagtatc agggtgctac ctaacaccaa tctttttcta caaagaaccc
    18001 attgcaacga cttttcatag ccattcctga gcttagcagc caaccatttc tcgttctcgt
    18061 tttgtttgag gtagttctcg cccgttagca acatgcgcgc cagcacaggg gtaagagtca
    18121 tagcaaccag caacgacata aaaagcgata caatgaatga aataccaagt ggtttaagca
    18181 ttcgcccttc catgccgctc aagaaaaaca agggaacaaa ggctacaata ataatcaaag
    18241 ttgcatttag gattgaagcc ctaatctcac gtgaagcttc ataaacaacc gtaaagctcg
    18301 attgcctttc gtgctcaggt ttctccctat tttgcctcaa tcgcttgtaa acattttcaa
    18361 catcaattat agcatcgtca accagcgaac caatggcaat tgccattcca ccaagactca
    18421 ttgtatttat gttgataccg agcaatttca tcaccacaaa ggtggcaagt aacgaaatgg
    18481 gtatggcaag cagcgatatt attgttgtcc gaaaactacc caggaaaagt agaagaatta
    18541 tggttacaaa gattgcaccc tcaattaatg cctgctgaac gttacccact gagcgctcaa
    18601 taaaatcgga ttgacggaat attttggtgt taagtttgac atcggggggt aacgattttt
    18661 tcaaattttc cagattagcc tctattttac gagttacctc aagcgtatta atattgggtt
    18721 gctttgagat tgatagaata attgccggcg atgcattgct agatgcataa cccattttag
    18781 tagcaggtgc aatacggatt tcagctatgt cgctaatctt tacaggcatt ccgttacggg
    18841 tagcaaccag tgtttgacca agctgctcaa tatcggaagt gcgcgctatt cctcggacta
    18901 catactcgtt gccaaactcc cttatgttaa ccccattgga attcatgctg ataaatcggc
    18961 atgcgttggc cagctcatgc atactaacgc ccaaatgaag catgcgtgtg ggatttgcca
    19021 ccacctggta ttgcttgtaa tcgccaccaa taatggtaac ctgcgaaact ccaccagtgg
    19081 caagaatgag cggcttaacc gtccattctg caatagtccg aagttccatc atatttgtac
    19141 tatcggcctg caggccaatg aaaaagattt cgcccatcac actgctctgt ggcgcaagca
    19201 taggttggct tacgcctaag ggcatttgtt gggcaaccgc aataagcttt tcgctaacta
    19261 cctggcgtgc ctttaaaaca tctactcccc aatcgaactc tacccaaaca aacgagaaac
    19321 cctgggccga tactgaccta acccgcctaa catcggtagc gccattcaca gcggtctcaa
    19381 ttggaaatgt tactaacctc tcaacctcct ccgctgccat gccatgagca tcggtcatta
    19441 caacaacggt aggcgctgta aggtcaggaa aaacgtcaat gtccatattg gatacagttc
    19501 ggagtccaat aaccgcaagc actagggtgc ttataagtat gagatattta tttctcagcg
    19561 agaaaccgat tattctatcg agcatagcca atttgtttta gtgaacatga cccgagtgag
    19621 catcgagcgc acctgaactt tgtgccattt ttacatatac ggcaccccga gttacaatcc
    19681 gattgccagg ttgcaagccc gataaaacct caacccttga gccatcggta acacctaact
    19741 tcacctcgcg tttaatgaaa gtttccggag tgagctgcac catcacaaaa tggttaccct
    19801 gctcctccag gagcgctgaa accggaacag taagggagtt gcccttgggt tggcaaagca
    19861 ggtaaacatc aaccacttcg cctactggta atacctcaac attgggtaat tccagaacaa
    19921 ccggaattaa atggttttcc gatgatattg ttctgcctac tgcagctaac ctgcctcctg
    19981 atgatttaag gtttacgacc attccggaag agcttaccct aatatttgca tcggaaattg
    20041 agcttagtaa attcaggaaa cgaggctgaa cgtaacactg tatacttgaa acagatagtt
    20101 tactcagctt tactaaaggc tgacctgcgt ttacgtaatc gccatttgat acaaatacct
    20161 tcagaacctc agcatttgca gggcttacaa tttgttgacc ctcggctgaa tatccgtttt
    20221 caaaggcctt aaacgcagca agggctacct cgtagtcgct ttttgccttt tggaactcag
    20281 cagtggaaat aacctgctca gctgccaaag ctttttgacg ctcgtaagcg gacttggcaa
    20341 gctcatacct acttttagcc tcggcataac gtaaagcaaa gttatcgtcg gccatgccct
    20401 tggaaattaa gctaagcaaa acctccgact tacgaacctg cattccgggt agaacagatt
    20461 tttcggtata gctcactact cccgaaatac ctgccaccaa aaccacctcg tcaacctgtg
    20521 ccggcataac catgccggtt gtttttacaa cctgcccaat tggctgctgg gtaactattt
    20581 cagtagcaaa atcaacagcc ctagcctgct cctttgtaaa tttgatagtg tttgatgcat
    20641 ggctatgctg gggcgagtct gcccaatcgt gttcgcaatg ataaatggta aactgcccca
    20701 accggtaaaa tcctttaatg ccatttatta ccatttcaat tgaagcatcg gccttaccgt
    20761 ttacatcaaa ttgtactttt agcctgtaaa ttccatcgtt accagtattt aaagctgtaa
    20821 aggaatacga ttttccttcg gctgttacgt taagcattaa tgaatcaacc tttacaggtt
    20881 tgaagctctt catccctgtt acgtgagcgg taagctcaac ctccttgccg gcgataattg
    20941 ggttgccaac aatgtatatc tcaaggctat cagtataagc tgtaaagtgc gcaacttcct
    21001 cttcggcttg ttcagcttgt tcatgcgaat gcccttcatg gttttgactt tgttgccttt
    21061 gagtatcatt acaagcagta aaaataaatg tagcaagggc tattattaaa taatttttaa
    21121 ccattccaaa tcgaattaat tattaacgaa aaa
//
LOCUS       DAOSAL010000044        21062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_1782_length_21062_cov_51.822012, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000044 DAOSAL010000000
VERSION     DAOSAL010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 21062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21062
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1782_length_21062_cov_51.822012"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1580
                     /locus_tag="PK049_07305"
     CDS             <1..1580
                     /locus_tag="PK049_07305"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOK61403.1"
                     /translation="TGEYISCPPFDVEIENNSLGTNLTFEYDFGDGDDSTTTSHANML
                     HTFHNLSNQTQPYQIKLRTVTEFGCDDSVFQTIYVFPEVVASYVANPGYAACSPFEVN
                     FTNNTINARFYEWDFADGITSSFPTPRHSFLNFGETDSVFNVVLYASSEFGCFDRDTQ
                     QIIVYATPIANIAAEPPLQVFPNATFNIYNQSSPAASSWNYNWAFGDNTFSTSKDPLT
                     KTYTRWGPKEQDFRYRITMRVDAPHGCWDADTVLVYLLPTEPEAFFTSSVDSACAPME
                     VQLVDASQFADSYLWEFGDGATSTERNPRHTYTEPGYYTVKLTVTNDAGSRFYYKIFR
                     VYESPEASFAIFPQRVMLPDATIHAYNLSTQFTRCQWDFGDGYQTTERDPVHTYDRLG
                     EFRVSLWAYMDYGNDVCVDSISKFPAVWVEGVGYLKFPNAFKPNLAGPNGGLYDAIDY
                     KNDVFHPYHYGVVEYKLMIFSRWGEQIFTSTDVNIGWDGYVNGKLAQQGVYMWRAIGK
                     FTNGKPFDMKGNVTLLR"
     gene            1594..2580
                     /locus_tag="PK049_07310"
     CDS             1594..2580
                     /locus_tag="PK049_07310"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HOK61404.1"
                     /translation="MNAIRRYVILVVFTFSAFLGFSQDMQFTQFYANPLYLSPSFTGG
                     VVGYRVAANYRNQWTFVPGTFNTFSVSWDHNSPAFRSGFGIMAMRDQAGDGNLANTRM
                     SVLYSYDIEPFPDWHLRPGLAFFLQQISIDFSKLIFSDQLTSTPMPPTSVTQPGKDAV
                     WDIDVSSSLLAYSDNIWLGVTWDHMLKPVATFYDDNSRLPYKITVHGGYRYITKGFLL
                     SRIEESFTVAFNYRVQDIYRQLDLGLYFYSQPISFGVWWRGLPKNSVGRRVDALAFMV
                     GYKWENVSMGYSYDFTISRLGLASGGSHEISMIYEFRLTPKKRWKAIPCPTF"
     gene            complement(2577..4925)
                     /locus_tag="PK049_07315"
     CDS             complement(2577..4925)
                     /locus_tag="PK049_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61405.1"
                     /translation="MKAFRISVFLLLIAWLPFYSSGQTKTEIDETYRDANSYFYFEDY
                     EEALALYQKIYKFQPDNYNLNYRIGFCYLNIPGSKHKSIQFLEKAVQNTTTRYNEESI
                     TEQRAPIDAIFYLGNAYLTVNRIDDALKAYEKFYSLTKGKSDWDFDYLNHQINVAKNS
                     LKIQRNPVDFIMANLGEKINDRFPNFNAVVSGNGKVLAFTTKLKFYNAINISRKNEKN
                     QWEKPVNITLDLQTDISCSTLCLSYDGNELYLFRDDNHDGNIYVTRFNGKKWLPIKKL
                     NPNINTEAYETHASLSPDGKQLFFTSNRKGGFGELDLWVSTRTSGDDWGPARNLGATI
                     NSPFNENTPFISTDGTTLYFSSEGHENMGGYDIFVSQLSGKGDWTTPINLGYPLNTTD
                     DDLFYHPIEDGATALIARFDDNSFGETDIFEIELFIPRFRKSIVPKTVAQERTTDKTF
                     ARLIVDTLSTKGMASIDANEAITKIDISGNNTLKLFLNGKAYGVIPSRIEELAEAKRV
                     SETENAAKAYTEETDNQTPSPISSNSATDATFKNNFGDTTPRSQWPTDTPTVKQKAFE
                     LNNAGINDDSYFSEILLLLTPNEKQQDILPVLKRNWKFSAENLNETIIHFASSFKTDK
                     ELNTIIYSLTELYDKIGNLSNTQSGKKEQKFAKNNRTLIIKAYNELINKASKQLGNDL
                     ALVMLKNKGIGNLTQLIELYSKQNPEGFNANREELTLLLARMAISEYVALPNEQKISL
                     YNSSTGKTSVEPSYIWLWVLLTLIAMGVIIYLGIEYYKKKTS"
     gene            complement(4929..6218)
                     /locus_tag="PK049_07320"
     CDS             complement(4929..6218)
                     /locus_tag="PK049_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61406.1"
                     /translation="MRQSIVLMVLLLISFSSSSAQRSSYYRRIFVDAEYYMLYEEYRD
                     ALPLYQELYKAYPNNYNLAYRIGLCYLNIPNEKAKSLPYFEKAITSISDSYKEGYFTE
                     TNAPKDAYLHYGRALRILGQFDKAQKAFETYKNLLKPNEITELRIVRKELEALDNARY
                     FTSNPLKHRMTPVGRNITTRFPEVNPVSDSSGNTLVYTSVQRFYNAILISNRGNEFWN
                     NPLNLNPQLYADGDIRTVGITSNGNVLLLSRNDNDIYNLYYSTYNKETNSWAPITKFP
                     KEINTSKSWQTFGSLSRNGDTLYFSSNREGGFGGFDIWMSIKTSNGWSSPINLGKNVN
                     TPFDEIAPFLTEDGKRLFFSSNGLKTMGGFDIFYSDYENKAWGIPINVGYPLNTTDDD
                     TFFFPIKNGKEGYYSKNSESKGDEDIYHVALDIKSPR"
     gene            complement(6530..7843)
                     /locus_tag="PK049_07325"
     CDS             complement(6530..7843)
                     /locus_tag="PK049_07325"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HOK61407.1"
                     /translation="MAEVKIVLPAMGEGVTEAKITKWLVKPGDLVEVDQPLVEIATDK
                     VDSEVPSTSKGKVKQLLFNEGDSPQVGQAICILEVEGVIAEGSKTHVAPAEPKSAQKE
                     KPIAKPIEITEKAEVKQEIKTLRYLSPLVRSMAKQENIALEELENIEGTGLDGRITKD
                     DLLKYIENRSNRISQIEAEAKERLQTETPIQYTSSTDTNISVLAHEVVPMDRMRKLIA
                     EHMVRSKQTSAHVASFIEVDVTNLVNWRETNKDKFQKTYGDKITLTHLFAQATIAEVK
                     RYPLLNSSVEGDNIILKRDVNLGIATALPNGNLIVPVVKRAETLNLIGIAKSINDLAQ
                     RARDNKLKPDEIVGGTFTITNLGSFDTLTGTPIINQPQVAILAIGAVKKRPVVIETPS
                     GDMIAIRHICILSLSYDHRVIDGALAGQFLKSLRDRLENFTPSEI"
     gene            complement(7949..9883)
                     /locus_tag="PK049_07330"
     CDS             complement(7949..9883)
                     /locus_tag="PK049_07330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOK61408.1"
                     /translation="MELSVVVVNYNVRYFLEQCLVSVFAALKGIEGEVWVVDNASTDG
                     SVEMVKLKFPQVKLISNQTNLGFSKANNQAIKLAKGRYTLLLNPDTVVQEDTFRECIN
                     FMDAHPDAGGLTVKMIDGKGRYLPESKRGFPSPWASFCKIFGLTSLFPKSKLFAHYYL
                     GHLDKNATHAIEIMPGAFMFLRSEVLNQVGLLDEQFFMYGEDIDLSYRILQHGYKNYY
                     YPKCQIIHYKGESTKKGSLNYVIVFYKAMILFAQKHFSKQKQNHFVTLIKLAVAIRAT
                     ASIIKRLIIKVWLPFTDFALMFLGAYLLIPWWEQFRHGVTNIYPRDILYTLIGFYLLT
                     WIMSLWLYGAYDKPQNLKSGAKGILYGTIAILVVYSILPQNFRFSRAIILILSAWSLL
                     SITLNRILASTFIEGLLKWKARKKSVCFIGTASELKGSEELLIQAGFHRNNITHLLPE
                     EVLDEHQRAKKEIIADAIKYKNISEIIFGTEGIPMTSIIHAMMYFSEFDIDFKIALHG
                     GDSIVGSSSVEAQGELYTLEVIPLAKPVARRQKRIFDILSSALIIVLFPILWIFLVKP
                     FMVLKSALQVFKGEKTWIGYNHPNISKQNSVLKPSVYLYSKWGKGLTSNVGLDFYYAQ
                     HFSITLEVSELIRNIFKTQD"
     gene            complement(10125..11336)
                     /locus_tag="PK049_07335"
     CDS             complement(10125..11336)
                     /locus_tag="PK049_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOK61409.1"
                     /translation="MINDLDQILSASAKRMKRSVIRELLKMTQRPELISFAGGLPSPE
                     SFPLEQLKQVVVEVLDTDSARALQYSETEGDKRLREILVEKYRKEGLNININNLIIST
                     ASQQALDLIPKIFVNPGDKVICGLPSYLGGISAFATYGADLVGIELDDHGMRSDLLEQ
                     KMQELQRKGQKPKFIYVIPDFQNPTGICMPERRRLEIIEIARKYNVLIVEDSPYREVR
                     FSGTPQRTMYELDGTGQVILLGTMSKIFVPGFRLGWIVAHEEVIDKLVVAKQNTDLCT
                     SSFVQKIAAKYFDKGYFEPNLAKTNAMYKEKRDAMVEAFHKFMPKGVKWTEPEGGLFL
                     FVTLPEYIDAEDLFKIAIEENVAFVPGTVFYCNGEGKNTLRINFSFMSKELNIEGSRR
                     LANAIKRMIKE"
     gene            complement(11785..12513)
                     /locus_tag="PK049_07340"
     CDS             complement(11785..12513)
                     /locus_tag="PK049_07340"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOK61410.1"
                     /translation="MFKYCLTLIIAATISLFAVAQSNISDLIANGNQKIKEKDFKGAL
                     AEFKNALNVSPNDSLALNGIIRANTLLEDYKEAQRWIAIALEAYPNNPDFLFRRGIIQ
                     NLNGYHDKAIEDFESALGQNPNTSLKVQILLNKASAHFKIEQFAESLADYNKVVELDP
                     RNFNAYNFRGLVNFRLGYYIDAVTDYTTAIDLDPTSPLPYYNRGMSLLKLNENQKACA
                     DFRKACQMGYMTSCKMVVAECGAR"
     gene            complement(12570..13199)
                     /gene="recR"
                     /locus_tag="PK049_07345"
     CDS             complement(12570..13199)
                     /gene="recR"
                     /locus_tag="PK049_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005468795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOK61411.1"
                     /translation="MQTHTYPSKLLERAVEELAKLPGIGNRTALRLALHLLRQPQADV
                     EQFLTAIGEMKTNIHFCSVCHNISDTDICSICSSPSRDKTTICVVETIKDVMSIENTM
                     QYKGVYHVLGGVINPMEGIGPNDLTINSLVERLSTSEVNEVIMALSATTEGDTTNFFI
                     YKKIKNFPVMVSTLARGVAFGNELEYTDEVTLGRALLNRVRFDNSFSVE"
     gene            13353..14816
                     /locus_tag="PK049_07350"
     CDS             13353..14816
                     /locus_tag="PK049_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007479058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOK61412.1"
                     /translation="MSTSLIIAVIIGYFVTLFVISYFTSRNSNNDTFFIGNRKSPWYL
                     VSIAMIGTSISGVTFISVPGWVLTSQFSYMQMVLGYLLGYFVVANVLLPLYYRLNLKS
                     IYTYLDARFGFYSYKTGAFLFLVSRVIGSAFRLFIVSNVLQLSIFNALGVPFWVTVSI
                     TVLLIWLYTRKGGVRTVLWTDSFQAIAMLTAVSLCVYYISSDLNLSLPGIVKAVSSSP
                     LSRVWFFDDPNQRFYFWKQFLGGAFITIVMTGLDQDMMQKNLSCRNYKDARKNMYWYG
                     FAFLPVNLLFLSLGVLLHTFAVQKGIPLPALSDDLFPTLATQGYLPQIVGVLFFLGLI
                     AAAYSSADSALTALTTSFTIDVLNTKGMDEDRLRRVRQWVHVFISILMILVIIVFNAF
                     NDRSVIDAIFTIAGYTYGPLLGLYAFGLFTRFQVNDKLVPFIAVFSPLACYLLNKYSI
                     LLFNGYKFGFELLIVNGAIVFLLLLITRKRGVLVQKV"
     gene            15259..15792
                     /locus_tag="PK049_07355"
     CDS             15259..15792
                     /locus_tag="PK049_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61413.1"
                     /translation="MKFKCFYFGLFIIPSLTLNAQESMFLQKNHSVSMEVLGLTYNYE
                     SPIAKRVTLIGRLGMQGGAGYSSSLGSYYAISPAISFETRFYYNILKRESSGKNIKGN
                     SANFLSLSTWYISNPVIEHKSSGADFFAVTPQWGVRRVYWKHFMLEAGAGISYFFNLE
                     GDSKTNLMLNLRLGYMF"
     gene            15834..16508
                     /locus_tag="PK049_07360"
     CDS             15834..16508
                     /locus_tag="PK049_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61414.1"
                     /translation="MDEFMKTLSFIIGLIFVSVSAKGQELYQKSWLIDVGLGSPMLKK
                     GETNPVQARISVGYEIQFTESGLLGLAMRGGYAITASHAYNINETGLPSTDHELLYSY
                     TGNSFFAAVSPMLIFEQYDSNVDRIFAELELGLASHGVNIKFYEPNPVTSSNWVYFKG
                     FFALKIGLRVAWEKNSKSRAMSIWGALHNQSPIDAIDKSIPVEWPYKFNEDLNSQWQV
                     GISVYL"
     gene            complement(16572..18383)
                     /locus_tag="PK049_07365"
     CDS             complement(16572..18383)
                     /locus_tag="PK049_07365"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HOK61415.1"
                     /translation="MEIKRTFDILEAGKDTFENRTVIAVKKNGVWDEYTIERYSQMAH
                     NLSYGLLSMGLRPGDKVANISGNRPEWNIVDMGITQAGMVHVPIYPTISTDEYKYILK
                     HAEVRAIFVGDKALYQKLQPLTADLENFIGIFSTVENGDIRSWNEVLERGKQTAHEWV
                     EELFKIKASVQPDDIATIIYTSGTTGVSKGVMLSHSNFLSNVEACKELFPLENGDIAV
                     SFLPLSHVFERMVNYYYQSIGVSIYYADNLATIGDVIKEVKPHIFMTVPRMLEKTFDK
                     IIAVGKDLTGLKKQIFFWAVNLGYKYNEVRNSWYYRIRLNLARKLIFSKWQKALGGRL
                     KLIISGGAALQPRLMRVFWAAGIPVFEGYGLTETSPVIAVNHPASPGNIRIGSVGPVV
                     PNVMVKIADDGEILMQGSSLMKGYYKAPDLTQMAIDEEGWFHTGDIGHLDDGKFLTIT
                     DRKKEIFKLSNGKYVAPQLIENKLKESFFIEQCMIVGENEKFVSALISPNFSFLHDWC
                     SRHKIHFENNEELITIPEVIARYQKEITTVNKQLSDYEQIKRYRLVAEQWTPQTGELS
                     PTLKLKRKVLYERYEKILEEIYGHAKQTEVRGVNKEL"
     gene            18471..19346
                     /locus_tag="PK049_07370"
     CDS             18471..19346
                     /locus_tag="PK049_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007756741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HOK61416.1"
                     /translation="MKQVSFCLIVCLISVNVFGQDKHAYTLFNGKGEKVVYQSVVSAV
                     KNSDLLLFGELHDNPIAHWLELELTVDVFDATKGNIILGAEMFEADNQLILDEYLGGL
                     ISQQRFEAEVRLWPNYKTDYKPLVEFAKSKGLKFIATNIPRRYAAMVSAGGFDTLQSL
                     SEMAKSFIAPLPVEYDPNLGCYKGMLSMMGMPGKGKSNPEYFPMAQAIKDATMAYNIT
                     RNTSSGKVFLHFHGSYHSNNYEGIYWYVKRKMPNLKIVTIATVTQDDVNALAKENMGI
                     ADFIIVVPERMTRTY"
     gene            complement(19351..19482)
                     /locus_tag="PK049_07375"
     CDS             complement(19351..19482)
                     /locus_tag="PK049_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61417.1"
                     /translation="MRTNLDRYSEYENNLLNRVTTLLIIVVITAILGLVCVENFLLR"
     gene            complement(19537..19947)
                     /locus_tag="PK049_07380"
     CDS             complement(19537..19947)
                     /locus_tag="PK049_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61418.1"
                     /translation="MKAKFFLISALGLSTLFGCIEGDNYVRFNDYVTMEAGLLPDTMI
                     LNEPYNLQIRASAPNGCWYNVKVYLNVKSDSAYFFSAAATYENHGEPCTQQLVTHDTT
                     ITITPKVPKTHLMYFYNPTGNPQIRIDTIYIKAE"
     gene            complement(20518..20703)
                     /locus_tag="PK049_07385"
     CDS             complement(20518..20703)
                     /locus_tag="PK049_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61419.1"
                     /translation="MGAPWVELLRDTIRHAYGVTHVVSLTKPTAMFHPRPIVGSDFYA
                     TAYVIPCWWRNLINIME"
BASE COUNT         5555 a         4244 c         4443 g         6820 t
ORIGIN      
        1 taaccggtga gtatatatcg tgcccgccat tcgatgttga aattgaaaac aactcattag
       61 gtactaacct aacctttgag tatgattttg gtgatggaga tgattccact actaccagtc
      121 acgctaatat gctgcacact ttccacaacc taagtaatca aacccaaccc taccaaataa
      181 agctacgtac agttaccgag tttggttgcg acgatagcgt tttccaaacc atttatgttt
      241 tccctgaggt agttgcgtcg tatgtggcca atcccggcta tgccgcttgt agcccatttg
      301 aggttaactt tactaacaat accattaacg cccgattcta tgagtgggat tttgccgatg
      361 gcatcacctc gtcgttccca accccaaggc attctttcct caactttggg gagaccgact
      421 cagttttcaa cgtagtatta tatgcatcct ctgagtttgg ctgtttcgat cgcgatacac
      481 agcaaataat cgtttatgca acaccaattg ccaatattgc cgctgagcca cccttgcagg
      541 tattccctaa tgctaccttt aatatttata accagagtag ccctgctgcc agcagctgga
      601 attacaattg ggcctttggc gataatacct tcagtaccag taaagatcct ctcactaaga
      661 catatactag gtgggggcca aaggagcaag actttaggta ccgcatcacc atgcgtgtag
      721 atgcgccaca tggatgctgg gatgccgata ccgttttggt ttacttgcta ccaacagagc
      781 cagaagcttt ctttacaagt agcgttgatt cggcatgcgc tcccatggag gtgcaacttg
      841 ttgatgcctc ccagtttgcc gatagctacc tgtgggagtt tggcgatgga gctacatcaa
      901 ctgaacgtaa tcctcgccat acatacactg agccaggtta ctacaccgtt aagttaactg
      961 ttactaacga cgcagggtca aggttctact ataagatttt tcgggtttac gagagccctg
     1021 aagccagttt tgccattttt ccccaacgcg taatgttgcc cgatgccact attcacgcgt
     1081 acaatctatc aactcagttt actcgctgcc agtgggattt tggcgatggt taccaaacca
     1141 ctgaacgtga tccggttcac acctacgatc gcttaggcga attccgagtt tccctatggg
     1201 cctacatgga ttatggcaac gatgtctgtg tcgattcaat ttcgaagttc cccgctgttt
     1261 gggtcgaagg ggttggatat ctgaaattcc ccaacgcatt taaaccaaac cttgcaggac
     1321 ctaatggtgg tttatacgat gctatcgatt acaagaatga tgtattccac ccgtatcact
     1381 atggtgtggt tgaatataag ctaatgatat tctcacgctg gggagagcag atatttactt
     1441 caaccgatgt gaatattgga tgggatgggt atgttaatgg taaacttgct caacagggag
     1501 tttacatgtg gcgggcaatt ggtaagttca ccaatggaaa gccttttgat atgaaaggga
     1561 atgtaaccct tttgcgctaa tttagtccac gttatgaatg caattcgacg gtatgtaata
     1621 ttggttgttt ttaccttttc tgcctttttg ggattctctc aggatatgca gtttacccag
     1681 ttttatgcaa atcccttgta cttatcccct tcgtttaccg gtggagtagt aggatatagg
     1741 gttgccgcta actaccgtaa tcagtggacg tttgtccctg gaacattcaa cactttcagt
     1801 gtttcatggg atcacaactc tcccgctttc cggagcggct ttggaattat ggctatgcgc
     1861 gatcaggccg gtgacggcaa cttggcaaat acaaggatga gtgttttgta ttcatatgat
     1921 attgaacctt tccccgattg gcacctgcga cctggtttgg ctttcttcct tcagcagatt
     1981 tccattgatt ttagtaagct gatttttagt gaccagctca cctccacacc tatgccacca
     2041 acctcagtta cacagcctgg taaggatgct gtatgggata ttgatgtttc ctcttcgctc
     2101 ctggcatact ccgataacat atggcttggt gttacatggg atcatatgct caaacccgtg
     2161 gccactttct acgacgataa ttccaggctg ccatataaga taaccgtgca tggtggatat
     2221 aggtacatta caaaaggttt tttgctcagt agaattgagg agtcctttac tgttgccttt
     2281 aattatcgtg ttcaggatat ttaccgccag ctcgatttag gactatattt ctattcacaa
     2341 cccattagtt ttggtgtttg gtggagagga ctacccaaaa attctgttgg ccgaagggtt
     2401 gatgccttag cttttatggt tggttacaag tgggagaacg taagcatggg gtatagctac
     2461 gattttacaa tatcccgact tgggttagcc tccggtggtt cacatgaaat atctatgata
     2521 tatgaattta ggttaacacc taaaaagcgc tggaaggcca ttccttgtcc aacgttttag
     2581 cttgttttct ttttataata ttctattccg agataaataa tcacccccat tgctatcagt
     2641 gtaagcaata cccatagcca aatatacgaa ggttcaacac tggtctttcc tgtactgcta
     2701 ttgtagagcg atattttttg ctcattgggc aatgcaacat attcactaat tgccattcga
     2761 gccaacagga gtgtaagttc ctctctattt gcattgaagc cttcagggtt ttgcttgctg
     2821 tataattcaa tgagctgtgt cagatttcca atccctttgt ttttaagcat aaccaatgct
     2881 aggtcattgc caagctgttt tgaggctttg ttaataagtt cgttatatgc ctttatgatt
     2941 aaagttcgat tgttcttggc aaatttttgt tcctttttgc ctgattgagt attgcttaga
     3001 tttccgattt tatcgtatag ttcggttaaa ctataaataa tggtgttcag ctctttgtcg
     3061 gttttaaacg atgatgcaaa atgtataatg gtctcgttta ggttttcggc actaaatttc
     3121 cagttccttt tcagaacagg aagaatatct tgttgtttct cattaggggt tagtaaaagt
     3181 aatatctcag agaaatagct atcgtcgtta atgcctgcat tgtttagttc aaatgctttt
     3241 tgttttacgg taggggtgtc ggttggccac tgggaacgtg gagtagtatc gccaaaatta
     3301 ttcttaaagg tagcatccgt agctgaatta cttgatatgg ggctaggtgt ctgattgtcg
     3361 gtctcttcgg tgtaagcctt agctgcattc tcagtttctg atactctttt agcttcagcc
     3421 aactcctcta ttcttgatgg gattaccccg tatgctttcc cgttgaggaa tagttttaat
     3481 gtgttgttgc cgctaatatc aatctttgta atggcttcat tggcatcaat ggatgccatg
     3541 ccttttgttg aaagggtgtc aactattagc cgggcaaaag ttttatcggt ggtgcgttct
     3601 tgagcaactg tttttggtac aatgcttttt ctgaatcgtg gaataaaaag ttctatctca
     3661 aaaatatcgg tttcaccaaa tgaattatca tcaaagcgag ctataagggc tgttgctcca
     3721 tcctcgatgg ggtgatagaa caggtcatcg tcagtagtat ttaaggggta tcccagattt
     3781 ataggggttg tccaatcgcc tttccctgaa agctgcgaaa caaaaatgtc gtatcctccc
     3841 atgttttcat gaccctcgga gctaaaatag agcgtagtgc catcggtgct gataaacggt
     3901 gtgttttcgt taaaaggcga gttaattgtt gcgcccagat tgcgagctgg cccccagtca
     3961 tcgccagatg ttcttgtgct aacccaaagg tcaagttccc caaatccccc tttcctattg
     4021 ctggtaaaga aaagctgttt accatcggga cttaacgagg cgtgggtttc atatgcctcg
     4081 gtattgatat tggggtttaa ctttttaatt ggtaaccatt tcttcccatt aaaccgggtg
     4141 acatagatgt ttccatcatg attatcgtct ctgaacaagt atagctcgtt tccatcgtac
     4201 gagaggcata aggttgagca gcttatgtcc gtttgtaaat ctaaagtgat attaactggt
     4261 ttttcccact ggttcttttc gtttttacgg gaaatattaa tggcattgta aaactttagc
     4321 ttggttgtga atgcaagaac tttaccattg cccgatacaa cagcgttgaa gtttggaaat
     4381 ctgtcgttga ttttttcacc caggtttgcc attataaaat ccaccggatt acgttgaatt
     4441 ttgagtgagt ttttggcaac atttatctga tggtttaagt aatcaaagtc ccagtcggat
     4501 ttgccctttg ttaagctata aaatttttcg tatgctttta aagcgtcgtc aatcctattg
     4561 actgttaggt aggcgttacc caggtaaaat attgcatcaa ttggagcacg ttgctcggtg
     4621 atggattcct cgttgtacct tgtagtagtg ttttgaacag ctttttctag gaactggata
     4681 gatttatgtt tactgccagg gatgttaagg taacaaaagc ctattctata gttgaggttg
     4741 tagttgtcgg gctggaactt gtaaattttt tggtagagtg ctagcgcttc ctcgtaatcc
     4801 tcaaagtaga agtatgagtt tgcatcccga taggtttcgt caatttcggt tttagtttgg
     4861 cctgaggagt agaacggcag ccaagctatc agcagaagga ataccgaaat tctaaatgct
     4921 ttcattgact agcggggcga tttaatatcc agggcaacat ggtaaatgtc ttcatctccc
     4981 ttgctttccg aattctttga gtagtagcct tctttcccat ttttaatagg gaagaagaag
     5041 gtgtcgtcgt cggtggtgtt gagcggatag ccaacgttta ttggtatgcc ccaggctttg
     5101 ttttcgtaat cggagtagaa gatgtcaaaa ccacccattg ttttaagccc attggagctg
     5161 aaaaatagac gtttcccatc ctcggttagg aatggagcaa tctcatcgaa tggggtgtta
     5221 acatttttgc caagatttat gggcgaagac caaccattcg atgttttgat ggacatccag
     5281 atatcaaaac cgccaaagcc accttcgcgg ttactgctga agtaaagagt atcgccattt
     5341 ctggaaagtg acccaaaggt ctgccagctt ttggaagtat taatctcctt ggggaacttt
     5401 gtgattggag cccaggagtt ggtttccttg ttgtatgtac tgtagtacag attgtatata
     5461 tcgttatcgt ttcgggaaag caaaagaaca ttaccgttcg atgttatacc cacggttcta
     5521 atatcaccgt cggcgtagag ttgaggattg aggtttagcg gattgttcca aaactcgttg
     5581 cccctgttgc tgataagaat agcattgtaa aaccgctgta ccgaggtata tactagtgtg
     5641 ttgccgctac tatcggaaac cgggttgacc tcagggaaac gggtggtaat atttctaccc
     5701 acgggggtca ttctatgctt tagtggattt gaggtaaagt aacgtgcatt atcaagtgcc
     5761 tcaagctctt ttcttacaat tctaagttca gttatttcgt ttggtttaag taggttctta
     5821 taggtttcaa aggctttttg cgccttgtcg aactgcccca atattctcag tgcccggcca
     5881 tagtggaggt aagcatcttt tggcgcattg gtttcagtaa aatacccctc tttgtagctg
     5941 tctgaaatcg aagttattgc cttttcaaag tagggtaacg atttggcttt ttcgttcgga
     6001 atattcaggt aacacaggcc aatgcggtac gctaggttgt agttattggg ataggccttg
     6061 taaagttcct ggtatagcgg tagggcatcg cggtactcct cgtaaagcat gtagtattcc
     6121 gcatcaacaa atatacgacg gtagtaagag ctccgttgag cgctcgaact gctaaacgaa
     6181 ataagcagta aaaccattaa aacaatactc tgcctcattt gtcgaatact agtaaactaa
     6241 aatgtttttt ctactttaaa caagccaatg taaaacttta ttcttcaaat atattaccaa
     6301 taagttaaat atcacacaat attacggcat tttttacact ggcacaagcg ataatccctt
     6361 tgttttgtta cgtttattaa tgttttttgt ttccatggct atatggttgg ttctctttaa
     6421 ttttgataaa atgttgttat agattgataa aaaaagtcta ccgcgcctca ataaaaagga
     6481 tgagtttagc tggaactcat ccttttttat tttaaacgtt tataaaagtc tatatttcag
     6541 atggggtaaa gttttcaagc ctatcgcgta acgatttaag gaattgccct gcgagggctc
     6601 catcaatcac tcgatgatcg tatgatagtg aaaggatgca aatgtgacgt atggcaatca
     6661 tatcgcccga tggggtttca ataacaacgg gacgtttctt tacagcacca attgccagga
     6721 tggcaacctg gggttggtta ataattgggg ttcctgttag ggtgtcgaac gagccaaggt
     6781 tggtaatggt aaaagtccct cctacaatct catcgggttt gagtttgttg tcgcgtgccc
     6841 gctgggcaag atcgtttatg ctctttgcaa tcccaatcag gttgagagtc tctgctcgct
     6901 ttactaccgg aactatcaga ttgccattgg gtagagcagt agcaatgcca agattaacgt
     6961 cgcgctttag aatgatatta tctccctcaa ccgacgagtt aagcagtggg taacgtttta
     7021 cttcagcaat ggttgcctgt gcaaacaagt gggttagtgt aatcttatcg ccgtatgttt
     7081 tttggaactt atccttattg gtttcgcgcc agttaaccaa attggtaaca tcaacctcaa
     7141 taaacgaggc tacatgtgcc gaggtttgct tggatcgaac catgtgctca gcaattagtt
     7201 tgcgcatccg atccattggt actacttcat gcgcaagaac gctaatgtta gtatcggtgg
     7261 acgaagtgta ctgaatgggt gtttcggtct ggagccgctc cttggcctcg gcttcaattt
     7321 gagaaattcg attactacgg ttttctatgt acttcaggag gtcgtccttg gttattcggc
     7381 catctaaccc ggtaccttct atattttcca actcctcaag ggcaatgttt tcctgtttag
     7441 ccatgctacg caccaacggt gaaaggtaac gcaacgtttt tatttcttgt ttaacttctg
     7501 ctttttctgt aatctcaata ggcttagcaa taggtttttc cttttgtgcc gatttaggct
     7561 cagccggagc aacatgtgtt ttgcttcctt ctgcaataac cccttccact tcaagtatgc
     7621 aaatagcttg cccaacctga ggcgagtcgc cttcgttaaa aagtagttgt tttaccttac
     7681 ctttgctggt cgatggaact tcggaatcaa ccttgtcggt tgctatttct actagaggtt
     7741 ggtcaacttc aaccaaatcg ccgggtttaa caagccattt tgtaattttt gcttcggtaa
     7801 caccttcgcc cattgctggg agtactatct ttacttctgc catgttattt gaattttctt
     7861 tttcacctct attaacacct aattgtgtaa aaggttgaaa gtcatcagac caaaattgaa
     7921 gaaaatccag acaaaatggt ttttaatgct aatcttgggt tttaaaaata ttcctgatta
     7981 gctccgatac ttccagagtg atactgaaat gttgagcgta gtagaaatcg aggcccacat
     8041 tactagttaa acctttgccc catttactat acaaataaac cgatggtttt aaaaccgaat
     8101 tttgtttgga aatgttagga tggttatagc caatccatgt tttttcccct ttaaaaacct
     8161 gcagtgcact ttttaaaacc ataaatggtt ttacaaggaa tatccataga atagggaaaa
     8221 gcacgattat cagagccgac gataaaatgt cgaaaatcct tttctggcgc cttgctactg
     8281 gttttgccag tggtattact tcaagagtgt aaagctcacc ttgggcttcc acggaactgc
     8341 tgccaacaat tgagtcgcca ccatgtagag caatcttaaa atcgatatca aattctgaaa
     8401 agtacatcat ggcgtgaata atacttgtca tagggatgcc ctctgttcca aaaattatct
     8461 cggagatatt tttatattta attgcatcgg ctataatctc tttttttgct ctttgatgct
     8521 catctaaaac ctcctcaggg agcaggtggg tgatattatt acggtgaaaa ccagcctgta
     8581 ttaaaagttc ttctgaaccc ttgagctcac tagctgtgcc aataaagcaa acgctttttt
     8641 ttcgggcttt ccatttaagt aaaccctcaa tgaatgttga tgctaatatt ctgtttaggg
     8701 tgattgatag taatgaccat gccgatagga ttagaatgat ggcacgtgaa aatcggaagt
     8761 tctgaggtag aatggagtaa acaaccagta tggcaattgt gccatatagt atacctttcg
     8821 ctccggattt taagttttga ggtttatcgt atgctccata cagccaaagg gacataatcc
     8881 aggttaaaag gtagaaaccg attagcgtat aaagaatatc gcgggggtat atattggtta
     8941 caccatgcct aaattgctcc caccaaggta taagtaggta ggctcccaaa aacattaagg
     9001 caaaatcggt aaatggtaac caaactttaa ttattaacct ttttattatt gaggccgttg
     9061 cccgtattgc gacggctagc tttatcaaag tcacaaagtg attttgcttc tgtttgctga
     9121 aatgcttctg tgcaaacagt atcatggctt tatagaatac tattacgtaa ttcaaactac
     9181 ccttttttgt gctttcgccc ttgtaatgta ttatttgaca ttttgggtag taatagttct
     9241 tatatccgtg ttgtaggata cggtatgaaa ggtcgatatc ctctccatac ataaagaact
     9301 gctcatcgag cagtccaacc tgatttagaa cttccgaacg aaggaacatg aaggcgccag
     9361 gcattatttc tattgcatgg gtcgcatttt tatccaaatg gccaaggtag tagtgtgcaa
     9421 aaagttttga tttagggaaa agtgaggtta gcccaaaaat tttgcagaac gatgcccaag
     9481 gcgaagggaa ccctcgcttg gattcgggta ggtagcgccc cttcccatca atcatcttaa
     9541 ctgttaaccc gccggcgtca ggatgggcat ccataaaatt tatgcactcc ctgaaggtgt
     9601 cctcctgaac aaccgtatcg gggtttagta aaagggtata tcttcccttt gcaagtttta
     9661 tggcctggtt gttggctttg gaaaagccaa ggtttgtttg gtttgatatt agttttacct
     9721 ggggaaattt aagttttacc atttcaaccg aaccatcggt tgaagcattg tcaactaccc
     9781 atacttcgcc ttcaatccct ttaagtgctg caaatacact tacaaggcat tgctctaaaa
     9841 aataccgaac attatagttt acaactacaa ccgagagttc catgaatcag taaaacgccg
     9901 attaattttg tggaaatata caaaaatgtt gcaaaaatgc aggtattcct agcagtaata
     9961 cttaaaactt gctgatggga aggatttcgg ataaaaaagc gtaccaacag ggcaaggtaa
    10021 atgaggttta attccagtat aaagtccaaa aaaaagtgcc actgagcaat tattaacaaa
    10081 tttaaactca gtggcaatac taaaagatgt gtggatgata ggtattactc ttttatcatt
    10141 cgtttaattg cattggcaag ccttcggctt ccttcaatat ttagctcttt cgacatgaat
    10201 gagaagttga ttcgaagagt attcttgccc tcaccattgc agtaaaatac agtgcctgga
    10261 acaaaggcta cattttcctc aattgcaatt ttgaataggt cttcggcatc aatatattca
    10321 ggcagggtaa caaataggaa gagtcctccc tcgggttcag tccatttcac acctttgggc
    10381 ataaacttat gaaaggcctc caccatggca tcgcgctttt ccttgtacat ggcattggtt
    10441 ttagccaaat tgggttcaaa gtatcccttg tcaaagtatt tagcggctat tttttgaaca
    10501 aacgacgagg tgcaaaggtc ggtgttttgt tttgctacaa ccaacttgtc aataacttct
    10561 tcatgagcta ctatccagcc caagcggaat ccaggtacaa atattttcga cattgtgcca
    10621 agtaatataa cctgtccagt gccatccagc tcatacattg tgcgctgtgg agttccgctg
    10681 aaccgcacct cacggtatgg gctgtcctca actattaaaa cgttgtattt tctagcaatc
    10741 tcaattattt ctagtcttcg cctttcaggc atgcagattc cggttgggtt ctggaagtca
    10801 ggtataacgt aaataaattt gggcttttga cctttccgtt gcaattcctg cattttctgt
    10861 tcaagcaaat cggagcgcat tccatggtcg tcgagctcaa tgcctacaag atcggctccg
    10921 tatgtggcaa atgccgaaat cccccctaaa tagctgggta gcccacaaat aaccttgtcg
    10981 ccagggttaa caaatatttt tggaataagg tcaagcgcct gttgcgatgc ggtggagatt
    11041 atcaggttgt ttatattgat gttcagccct tctttcctat acttttcaac cagaatctcc
    11101 cgaagccttt tatcaccttc ggtttcgcta tactgaaggg cgcgagcgct atcggtatct
    11161 aatacttcta ccactacctg ttttagctgt tcaagcggaa aactctcagg cgaaggaaga
    11221 cctccagcaa aagagataag ttcaggacgt tgagtcattt ttagcaattc ccttatcact
    11281 gaacgcttca tccttttagc gcttgccgat agaatttgat ctaggtcgtt aatcatggtg
    11341 tttttcttat aatatgttat taaaccaagg ctattacaaa atatctatta ctaaatattg
    11401 ctgctaagtt aaaaaatgta attagatatt tcaaaatttt tagaccgaga ttgttcagtt
    11461 tacatacaaa aaattaaata acttgtttct attggcttat agatgtaatc ccgactctaa
    11521 aaacgttact gcgttcatta tattaatagt tgaatttgtt aattgatgat gcttcgcttt
    11581 tgcttttcac ttttcactat tcctctaact tcctctaact tcttctaact tcttcaaatt
    11641 ctaactccat catattctgt ttattccgac tcattccggt tcattcctct aaaattagcc
    11701 ttgtttagtt catgatgttt gagttaaata aaaaagtccc caatagggac taatttttat
    11761 tgagcaatat atttattaac ttttctaccg ggctccgcat tccgccacca ccatcttgca
    11821 ggaggtcatg taacccattt ggcaagcctt acgaaaatcg gcgcaggctt tttggttttc
    11881 attcagctta agcaggctca tgcccctgtt gtagtagggt agtggagatg ttggatcgag
    11941 gtcgatagcg gttgtgtaat cggtaacggc atcgatatag tagccaagcc tgaagttaac
    12001 aagccctcgg aagttgtagg cattaaaatt gcgcggatcc agttccacta ctttgttgta
    12061 gtcggcaagc gattctgcaa actgttcaat cttaaaatgg gccgaagcct tattaagcag
    12121 gatttgaacc ttgagcgacg tgttagggtt ttggccgagg gcgctttcaa agtcctcaat
    12181 agctttatcg tggtaaccgt ttaagttctg aataataccc ctacgaaaca ggaaatcggg
    12241 attgttaggg tatgcctcaa gcgcaatggc aatccatcgt tgagcctcct tgtaatcctc
    12301 gagcagagta tttgccctga taataccatt taaagctagg gagtcgttag gcgatacgtt
    12361 gagcgcattc ttaaattcag caagggcgcc cttgaaatcc ttttccttaa ttttttgatt
    12421 gccgttggca ataagatcgc taatgttgga ttgggcaaca gcaaaaagcg aaattgttgc
    12481 agctattata agggttaagc aatacttgaa catgtttgta tagaataatg atgttttaaa
    12541 aatacaatct ttttgttaaa gttcaaaaat tattccacgg agaaagaatt gtcaaacctt
    12601 acccggttaa gcagggcacg gccaagcgtg acctcgtcgg tatactcaag ctcattgcca
    12661 aaagccacac ctcgggcaag ggtggaaacc attaccggga aatttttaat ctttttgtag
    12721 atgaagaagt tggtggtatc gccttcggtt gtggcgctta aagccattat cacctcattt
    12781 acctcgctgg tcgacagcct ttcaaccaag ctgttaatgg ttaaatcgtt tgggccaatt
    12841 ccctccatgg ggtttattac tccacccagc acgtggtaaa cacctttgta ctgcatggta
    12901 ttttcaatgc tcatcacatc ctttatggtt tcaaccacac aaatggttgt tttatcgcgc
    12961 gatggcgatg aacatattga acagatatcg gtatcggaaa tattgtgaca aacggagcag
    13021 aaatggatat ttgttttcat ttcgccaata gcagttagga attgctcaac atcggcttgg
    13081 ggctgcctaa gtaaatgaag ggctaacctt agcgcagttc tattccctat cccggggagt
    13141 ttagccaatt cctcaacagc tctctccagc agttttgatg ggtatgtatg ggtttgcatg
    13201 ttaatgtcgt ttgtttggca aatctagtta ataagagtag attattgcga tgatacatta
    13261 aacgattttc gctcaaatag gtttgcagcc ttgcttagtt tttgtaatgt gtagattttt
    13321 tcatacactt gttggctcaa atttattgcc gtatgtcaac aagcctgatt attgctgtaa
    13381 tcattggata tttcgttact ctctttgtga tttcctattt cacttcgcgc aattccaata
    13441 acgatacttt ttttattggt aaccgtaaat caccctggta tttggtatcc attgccatga
    13501 taggaacctc catatctggt gttaccttca tttcggttcc aggatgggtt ttaaccagcc
    13561 aattttcata catgcaaatg gtgttgggct acctgctggg gtattttgtt gtggcaaacg
    13621 tgctcttacc cctctactac cggttaaacc taaaatcgat atacacctat ttggatgcac
    13681 gctttggttt ttactcctac aaaaccgggg cattcctctt cttggtttca agagttattg
    13741 ggtctgcatt caggcttttc atcgtatcga atgtattgca gctctcaatc tttaatgctt
    13801 tgggcgttcc tttctgggtt acagtgtcca taacggtttt actgatttgg ctctacacgc
    13861 gcaaaggcgg tgtgcgcacg gtgctatgga ccgattcatt tcaagccatt gcaatgttaa
    13921 cagccgtttc gctttgtgtt tactatattt caagcgattt aaatctcagc ctcccaggca
    13981 ttgtaaaggc ggtaagcagc agtccgcttt ccagggtctg gtttttcgac gatccgaacc
    14041 agcgcttcta cttctggaaa cagtttcttg gtggcgcatt tataaccatt gtaatgactg
    14101 ggcttgacca ggacatgatg cagaaaaacc ttagctgccg gaactacaaa gatgcccgca
    14161 aaaacatgta ctggtatggt tttgcatttc taccggtaaa cctacttttt ctatcgcttg
    14221 gtgtgctttt gcacactttc gctgtacaga aaggcatacc tcttcctgca ttgtccgacg
    14281 atttgttccc taccctggcc actcagggat acctgccaca aatagttggt gttttgttct
    14341 tcctagggtt aattgctgcc gcatactcca gcgccgactc agccttaacc gctttaacca
    14401 catcgtttac cattgatgtc cttaatacca aaggtatgga cgaggataga ttacgaaggg
    14461 ttaggcagtg ggtgcatgtt tttatatcaa tactgatgat acttgttatc attgttttta
    14521 atgcattcaa cgaccgctca gtgattgatg ccatttttac tatagccgga tatacctatg
    14581 gtcctctact tgggctgtac gcgtttggtc tgtttactcg ttttcaggtg aacgataagc
    14641 ttgtaccatt tattgcggtt ttctccccat tggcttgcta tctcctgaat aagtactcta
    14701 tccttttgtt taatggatac aaatttgggt ttgaactttt aattgtgaat ggggcaattg
    14761 tttttctcct attgctcatt acccgtaaac gcggagttct tgtacaaaag gtttagagtt
    14821 taaaaataag gtgttttaat ttcgcttagt tacggatgct tgtcatgttg agtcccgaaa
    14881 tatcaggatt gaatcctgcg tcatatctat ctgtactctg tgttcgccga agcatctctt
    14941 tgtcctcttt cttttatccc ctttgatgag atccctcctg gtcctcggga tgacaaatgg
    15001 aattgttcgt gaaactattg tcatgttgag cgcaagcgaa acatctcata gtgtcgttga
    15061 gagatccttc gctacgctca ggatgacgaa tgaagtaatt gcatatatta acaaacgata
    15121 tatttttgtg agtttttaat tcgagtaatt cttattgttt taaaatattt tggaattgta
    15181 taaataaata tctttcaaaa taattaaatc ttgttttaat aattgtttaa taatcaattt
    15241 actaaatctt tttttattat gaaatttaaa tgtttttatt tcgggttatt tattatcccc
    15301 tcattaaccc taaatgctca agaatccatg tttttacaga agaatcattc cgttagcatg
    15361 gaggtgcttg gtttaaccta taattacgag agccctattg ctaaaagggt tactttgatt
    15421 ggtagattgg ggatgcaagg aggtgcgggt tacagcagct cattgggttc atactatgca
    15481 ataagccccg caattagttt tgaaacacgt ttctactaca atattttaaa gagggaaagt
    15541 agtggtaaga atattaaagg aaactctgct aattttttat cgcttagcac atggtacatt
    15601 tctaatccgg taatagaaca taaatcttca ggtgccgatt tttttgcagt taccccacag
    15661 tggggagtaa gaagagttta ttggaaacac tttatgctag aggcaggggc ggggattagc
    15721 tattttttca acttagaagg cgatagcaaa acaaatttaa tgttaaactt gagattaggt
    15781 tatatgtttt aatgtttcca gtattagttc atataatata ctaacatttt gatatggatg
    15841 aattcatgaa aacactttca ttcattatag ggctaatttt tgttagtgta agtgccaaag
    15901 ggcaagaact ttatcaaaaa tcatggctga ttgatgtagg cttaggatct ccaatgttaa
    15961 aaaaaggaga aacaaaccct gttcaagcaa gaatatcagt tggatacgaa attcagttca
    16021 ctgaatctgg attattgggt cttgccatgc gtgggggtta tgccataact gcaagtcatg
    16081 catataatat caatgaaaca ggtttgccca gtactgacca tgaattactt tatagttata
    16141 ccggaaatag tttttttgca gcggtgtctc cgatgctaat atttgaacag tatgatagca
    16201 acgtggacag gatatttgct gaattagagt tggggttggc aagccatggt gtaaatatta
    16261 aattttacga acctaatcca gttaccagtt ctaactgggt ttattttaaa ggtttttttg
    16321 cacttaaaat tggattaaga gtagcttggg aaaaaaacag taaaagtaga gctatgtcaa
    16381 tatggggagc cttgcacaat cagagtccta tagatgcaat tgacaagtca attcctgtcg
    16441 aatggccata taagtttaat gaggatttga actcacagtg gcaagtaggg atttcggttt
    16501 atttgtagta ttaattaaaa gcaaaagagg ctgttcttaa cagcctcttt tatttcataa
    16561 aatatacttc attataattc cttgttcaca cctcttacct cggtttgttt ggcgtggcca
    16621 taaatttcct caagtatttt ttcgtaacgt tcgtacagaa ccttacgttt tagcttaagg
    16681 gttggagata gctcgccggt ttgtggtgtc cattgctctg ctactaagcg gtaacgttta
    16741 atttgttcat aatcgctgag ctgtttatta acggtagtaa tttccttttg gtaccttgca
    16801 ataacctcag gaatggtaat cagttcctcg ttgttttcaa aatgtatttt atgtcggctg
    16861 caccagtcgt gcaggaagct gaagtttggc gaaattaatg cacttacaaa cttttcgttt
    16921 tcccctacta tcatacattg ctcaatgaag aacgattctt tcagtttatt ctcaattagt
    16981 tgtggtgcaa cgtacttccc gtttgagagt ttaaagattt cctttttcct atcggttatt
    17041 gttaggaact taccatcgtc caaatggcct atatcgccgg tgtggaacca gccctcctcg
    17101 tcaatagcca tttgggttaa atcgggggcc ttatagtagc ctttcatcag gctggagccc
    17161 tgcattaata tttcgccgtc gtcggctatt tttaccatta cattaggcac tacagggcct
    17221 actgaaccta tcctaatgtt gccgggtgaa gcagggtggt taactgcaat tacaggcgag
    17281 gtttcggtaa gtccatatcc ctcaaaaaca ggaatgccag cagcccagaa taccctcatg
    17341 agtcggggtt gaagagcagc tcctccacta atgataagct tgagccttcc accaagtgcc
    17401 ttttgccact ttgaaaagat taacttgcgg gcaaggttaa gcctaatccg gtagtaccag
    17461 ctattgcgta cctcgttata cttgtagcca aggttaactg cccaaaagaa aatctgtttc
    17521 tttagccctg ttaaatcctt tccaaccgca attatcttgt cgaatgtttt ctcaagcatg
    17581 cggggaacag tcatgaaaat atggggttta acttctttaa ttacatcgcc aatggtggca
    17641 aggttatcgg catagtaaat gcttacccct atgctttggt agtaatagtt caccatgcgt
    17701 tcaaatacat ggcttaatgg aagaaaactt acggcaatgt cgccgttttc aagggggaat
    17761 agttctttac aggcttcaac attactaagg aagttggagt gtgaaagcat tacccctttt
    17821 gaaacgcctg ttgtgcccga agtgtaaata attgtagcaa tatcatcggg ttgaaccgat
    17881 gcttttatct tgaaaagctc ctcaacccat tcatgtgcag tctgtttgcc gcgctccagc
    17941 acttcgttcc agctgcggat atcgccattt tctactgttg agaatatccc aataaaattc
    18001 tcgaggtcgg cggtaagggg ttgaagcttt tggtacaagg ccttatcgcc tacaaaaatg
    18061 gctcttacct ctgcatgctt tagaatgtat ttgtactcat cggtgctgat tgttgggtaa
    18121 atgggcacat gaaccattcc ggcctgggta atacccatgt caacaatatt ccattcaggc
    18181 cgattgccgc tgatgttagc aaccttatcg ccaggcctaa gtcccatgga cagcaatcca
    18241 tagctgaggt tgtgagccat ttgagagtat cgctcaatgg tgtactcatc ccaaacccca
    18301 tttttcttaa cagcaattac tgttctgttc tcaaaggtgt ccttgcctgc ttcaagaatg
    18361 tcaaaagttc ttttaatttc catgctttat cagataaagt tttacttatt tgaactattt
    18421 gctcagaaaa aggttaattt agtcccggcg acaaatataa actaatttct atgaaacaag
    18481 tatccttttg tttaatcgtt tgtttaattt ccgtaaacgt tttcggacag gacaagcacg
    18541 cctacaccct cttcaatggg aaaggggaaa aggttgttta ccagagtgtt gtaagtgcgg
    18601 taaaaaattc tgacttattg ttatttggag agctgcacga caaccctata gcgcattggc
    18661 ttgagctgga attaacggtt gatgtttttg atgccacaaa agggaatata atactgggtg
    18721 cagagatgtt tgaagcagat aatcaactta ttctggatga gtatcttggt ggcttaatta
    18781 gccagcaacg gttcgaagcc gaagttcgct tgtggcccaa ctataaaact gactataagc
    18841 cactggttga atttgctaaa agcaaaggtt taaaatttat tgccacaaat attccgcgcc
    18901 gctatgctgc tatggtttcg gcaggtggtt ttgatacgct acaatctcta tcggaaatgg
    18961 caaaatcgtt cattgcccct ttgccagtag agtatgaccc aaacctggga tgctataagg
    19021 gaatgctcag tatgatggga atgcccggta aagggaaatc gaaccctgaa tatttcccaa
    19081 tggcacaggc cattaaggat gcaactatgg catataatat aacccgaaac actagtagcg
    19141 gtaaggtttt tctacatttt catggttctt accactctaa caactacgag ggaatatact
    19201 ggtatgtaaa gcgaaaaatg ccgaacctga aaattgtgac aattgcaact gtaacccagg
    19261 atgatgtcaa tgctttggca aaggaaaata tgggcattgc cgattttatc atagttgtac
    19321 ccgaacgaat gacccgaaca tactaatccc ttacctgagc aggaagtttt caacacaaac
    19381 taaccctaaa atagctgtaa taactacaat tatcagcagg gttgttactc tgttcagcag
    19441 gttgttctcg tattcagagt atcgatccag attagttctc atggctatag tttcttttac
    19501 ttatactaca accatgaggg gttttaccct aaccggctat tcagccttaa tgtaaatagt
    19561 atcaatccta atttgaggat taccagtggg attgtaaaag tacattaggt gagttttcgg
    19621 cactttgggt gttatggtta ttgttgtgtc gtgagtaacc aattgttggg tacatggttc
    19681 accatgattc tcgtatgtgg cagctgcgct gaaaaagtat gcagaatcgg atttaacgtt
    19741 caggtaaacc ttaacattat accagcagcc atttggcgcc gatgccctta tttgaaggtt
    19801 gtatggttca tttaatatca ttgtatcggg cagtaatcct gcttccatgg ttacataatc
    19861 gttaaacctt acatagttat cgccctcaat acatccaaaa agggttgaca atcctaacgc
    19921 tgaaatgagg aaaaattttg ctttcattga gtttttcatt agtatttctg caaatttata
    19981 caaaaattgt accgtttggc tatttaacct ctaatttgtt gtatggcgtt ggtgaggtat
    20041 gaaaatacta ctcaaaaatt tgtcatgctg agctagccga agcatcacat tgcgtcttca
    20101 ttcttttatt cactttttgc tctttggaat gttctccttt tgtgatgcaa cgtttcagtg
    20161 ttgctctatg gaatgccata gcgtgcatcg ctattcgtgt gtaaagctat ggtgttacaa
    20221 atattacgcc cctacggggc tgggaacgtt gctcgttttt cgttgttcgt gaatcgttgc
    20281 tcgttgttcg tgaatgcctt tttgtgaaac actgtattac acagtgtaac tctgtgatgc
    20341 ttgtttttac taagctcatt agacgattgt catgctgagc tcgtcgaagc atccctttgc
    20401 tctattgccc ttcaatcctt taatgagatc cctcgactaa gctcgggatg acaaaaaccc
    20461 catcattcca tttgccccct tgtcttgctg agttagccga agcatctctt tgtactttca
    20521 ttccattata tttatgagat tcctccacca acacggaatg acataggcag tggcataaaa
    20581 gtctgacccg acaatgggtc gggggtggaa catggcggtg ggtttagtaa gcgataccac
    20641 atgagtcacg ccgtaggcgt ggcgaatagt atcgcgtagc agttccaccc acggggcacc
    20701 catcgggtcg ggcttttcag ccttgatttt ctttggttct ttcttgtatc aagacaagaa
    20761 agaacatgta tgccgctctt tgctaggctt ttccgctgcg atgcaacgtt tgctccttgc
    20821 tctttggatt atgtattgcg tgcatcgcaa cacttgtggg attagccata gtgttacaaa
    20881 tattacgccc ctacagggct ggaaacgttg ttcgttgttc gtgaatcgtt gtttgttgct
    20941 cgtgaatgcc gcgccacata ctctgtgtaa cttcgtgtta catagtgtaa ctctgtgatg
    21001 cttgctgttg ctcggttcat gagacggttg tcatgctgag cctagccaaa gcatctcttt
    21061 gt
//
LOCUS       DAOSAL010000045        20366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_1861_length_20366_cov_51.809167, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000045 DAOSAL010000000
VERSION     DAOSAL010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 20366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20366
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1861_length_20366_cov_51.809167"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="PK049_07390"
     CDS             <1..714
                     /locus_tag="PK049_07390"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOK61420.1"
                     /translation="FAQKAKRVVTVSQFSRTDIANNFNIDPNKIDVAYNGASNSYKPL
                     SESEIQRVRESISEGKPYFLFVGAFNPRKNVARLIEAYDIFRKRTGSAINLLLVGEPM
                     FKTNDIGIAYKNCNHKNDIKFLGRKGLDELTKITGAAHAVIYPSTFEGFGIPLLEAMK
                     CGIPIAASNVTAIPEVTGNSAIYFDPYSVDEMADAMQRVAYDTELRETLSANALNRQQ
                     QFSWDNTAQVLYSSIEKCF"
     gene            717..1295
                     /locus_tag="PK049_07395"
     CDS             717..1295
                     /locus_tag="PK049_07395"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008763678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOK61421.1"
                     /translation="MLKQYLNVNVIEAGCDEAGRGCLAGPVFAAAVILPPDFNDPLIN
                     DSKQLNHKQRMQLRKVIEHYALDWAVASVSNQEIDKINILNAAIHAMHIAIGKLSKLK
                     PEHLLIDGNRFKPYPGIPHTCIIKGDSKFLSIAAASILAKTYRDEFMVNISKEYPLYN
                     WDINKGYPTKKHRESIAKHGTTPYHRLSFRLL"
     gene            1371..3521
                     /locus_tag="PK049_07400"
     CDS             1371..3521
                     /locus_tag="PK049_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOK61422.1"
                     /translation="MIKRLTTLLLGFLLLVSSAIKADEGMWLLPLLQKYNIQAMQQKG
                     FKLTADDIYNLNQASIKDAVIIFGRGCTGEVVSDQGLVLTNHHCGYGAIQQHSTPEHD
                     YLEDGFWAKSFEEEIPTPGLTATFLIRIEDVTDQVNAAFKPEMSEADRDKAAMEIGRK
                     IAKQATDGTHYTASVRSFFGGNQYFLLVYEVFNDVRLVGAPPSSIGKFGHDTDNWMWP
                     RHTGDFSVFRIYASKDNKPADYSRENVPYKPKHFLPISLKGVNNGDFTMIMGYPGSTQ
                     RYMTSVEVQERINILNANRIYVRGIKQEIWLKDMQADKAVNIKYASKYAGSSNYWKNS
                     IGMNKALKRLKVYDFKKQQEEEFTKWVLASPERQQKYGEALSLINEGVTGRKEYLHAS
                     QYISEALFSGTEIIGMAAMTRSLVDALKGNKTEEVTKISKSLKARSENFYKNYNTPTD
                     QKTAKAMFRIFMENVPLTYQPDIFNTIRTKYKGDVDKFVDQMFAKTMFTDPAKFNAFL
                     DKPSLKVLEKDPVYVAAASISNKYEEISKVMAPFNEKLQRGQRLYIAGVLEMNEGKAM
                     YPDANFTMRLTYGEIKDYYPMDAVHYDYLTTLDGVMEKEDPDNWEFVVPAKLKELYNS
                     KDYGRYGKDGKMPVAFISTNDITGGNSGSPVMNGNGELIGIAFDGNWEAMSGDIVFEN
                     KLQRTISVDIRYVLFVIDKYAGATRLIDEMKIIE"
     assembly_gap    3569..3668
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3851..4369
                     /locus_tag="PK049_07405"
     CDS             3851..4369
                     /locus_tag="PK049_07405"
                     /inference="COORDINATES: protein motif:HMM:NF038262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HOK61423.1"
                     /translation="MEKGEVLINHYGPLSYEEIGFLLNKMTSLLERYNFGITIRKKVY
                     SAMVESLENIYKHQDVIKGSNNYQPKFSLVLEKDKVDLSCSNSLLKVKIGPLKERLEK
                     VNQLDKAGLKEFYKEVILSGNVTQKGGAGLGIINIAKVTENKLEFTFDDIDEQYTYFT
                     LRIKVSLQNQNN"
     gene            4375..4785
                     /locus_tag="PK049_07410"
     CDS             4375..4785
                     /locus_tag="PK049_07410"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOK61424.1"
                     /translation="MEPLYIEPSEFTPRVFFDPENSVFEISGFSRPENTVGFYRPLIK
                     WLEDYEDNVLAKNTNFNKSNLVLNLKLTYFNSASSKFLMDILMVFMKFHSKGNAVEIN
                     WYYDEDDDEIFESGEEISEMINFPFNFIPITPNS"
     gene            4797..5198
                     /locus_tag="PK049_07415"
     CDS             4797..5198
                     /locus_tag="PK049_07415"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOK61425.1"
                     /translation="MNPLVLQASNTTPYIDFNPSENRLTISGYSRPENVRDFYMPVMD
                     WLESFSNELKSKMLKEEQIQPIEFQFKLVYFNSSSAKFLYDIIIILNQIQKAKVPLNI
                     NWYYDKDDSELREAGEDLSELAQVPFNFVTY"
     gene            5416..7110
                     /locus_tag="PK049_07420"
     CDS             5416..7110
                     /locus_tag="PK049_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOK61426.1"
                     /translation="MTEHELTLGDSGERPKNFLEEIIEEDIRTGKHGGRVLTRFPPEP
                     NGYLHIGHAKSICLNFGLAQKYGGKTNLRFDDTNPVKEDVEYVNSIKEDIRWLGFEWA
                     NEFYASDYFEQLYEWAELLIKKGLAYVDDQSQEEIRINRGTVTQPGKESPWRNRSVEE
                     NLDLFRRMRAGEFKDGEKVLRAKIDMAHPNMLLRDPIMYRIIHAEHHRTGDKWCIYPM
                     YDYAHGQSDSIEQITHSICTLEFDVHRPLYDWFIEKLEIFPSKQYEFARLNLSYTVMS
                     KRKLLQLVQEKVVMGWDDPRMPTICGLRRRGYTPEAIRYFADKVGVAKRDNVIDLSLL
                     EFCIREDLNKRAERRMAVLNPLKVVITNYPEGQVEELEAVNNPEDETAGKRLIPFSRE
                     IYIERDDFMENPPKKFFRLAPGQEVRLRYAYFIKCEEVIKDSQGNVIELHCTYDPASR
                     GGNSPDGRKVQGTIHWVSIPHAVKAEIRLFDRLFSKPDPDDVPEGQDWKSSLNPESLT
                     VIEGYLEPSLKHAKQLDKFQFERLGYFCVDYDSTPEHLVFNRTVTLKDTWAKLAGK"
     gene            7212..7284
                     /locus_tag="PK049_07425"
     tRNA            7212..7284
                     /locus_tag="PK049_07425"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7245..7247,aa:Gln,seq:ctg)
     gene            7351..8457
                     /locus_tag="PK049_07430"
     CDS             7351..8457
                     /locus_tag="PK049_07430"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOK61427.1"
                     /translation="MTNVIPIYHIGLIQAWFAALLVYRKPNKGAADRLLVIWLIVIGI
                     EMLYSLLNAKYLTWLPDLIIIPLSYGPFLFFYTKLLTSKKPTTLRNIHRHLIPFYLFI
                     LIAAVWGKPIDIDSVNFYSKGPITWLAIFNYALFLIVILYYWRASLILIRKHNRTLGD
                     HFSYRSETIMLNWVKNIAIWILSGFFASGITFIIFSTQNIFPFNPIELFHLGLLVFTF
                     SISYYGIHQPVLYQYQKPKQIEVTEDLQTKTSQKLSDLLYEHMVKNKPYLNNELTIQD
                     LADQLKVSSQEISSYLNKELGVNFFNYINGFRIEEAKQRLTNPNFDNETLLGIAYDSG
                     FNSKTSFNTLFKKETGMTPSEYKKAFRSSTKKNT"
     gene            8620..9942
                     /locus_tag="PK049_07435"
     CDS             8620..9942
                     /locus_tag="PK049_07435"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOK61428.1"
                     /translation="MRKVIFALLICMVAMPSFAQENQEKKTNEEVKTGWTVGVLPALG
                     YDSNLGFLYGAIVNSFDFGDGSRYPDFNHNLYLQLSAYTKGSMDAIAYFDSYTLIPNK
                     HFIGRLSFNRNRTYPFYGFNGTQTVYNADFENINATDDFISQVFYKYDRKLAKADFLL
                     EDRIADSKFKWIAGFDLGWYDVATVDIDHLNKGRDDDELIPDVPILYDQLVSWGIISA
                     DEKDGGFDNSVKFGLVFDTRDRLTNPMKGVWTELLFRASPKFIGNNDNYGRVSLIHRQ
                     YLTLVKEKLSFAYRLWYEGNLGNVPFYARQHLTTSNYYEGFGGAQTVRGVLMNRVVGQ
                     HTAIGNFELRWKAYRFHAIGQNFYLGFNLFADAGKVLKGYDMDLTNVPVAQRNQLFSN
                     SYKDLYTTFGAGLKIVMNENFVVSADYGVAMDKNYGSSGLYVLVGYLF"
     gene            10011..13124
                     /locus_tag="PK049_07440"
     CDS             10011..13124
                     /locus_tag="PK049_07440"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOK61429.1"
                     /translation="MLQAQNWQDPKVFAINRLPSHTLLVPFPEKSSSISYIFNSEFVQ
                     SLNGDWKFMLINNPLNTPENFSNVEFTDSEWSTIPVPSNWQTKGYGTPIYSNQIHPFP
                     ANPPFVPNEGNETGLYRHTFRIPENWNSSRVIIHFAGVQSAIDLWINGHYVGYSQGSM
                     TPAEFDITPYITAGQNLLAARVINWSDGSYLEDQDFWRLGGIFRDVFVYAQPQNALWD
                     IEVNTSFSKSYTEATLSVSGVLTNTTSQLPNISLSLFDSNGIAVFEKTVQPKLSSGIA
                     TFKVDEIVNNPQLWSAETPNLYQLIVEIEGRYYQQLVGFRDVKVENGQLLVNGQPIKI
                     KGVNRHEFDPYNARTVSYELMEKDVVLMKQNNFNAVRTAHYPHHPYFYELCDRYGLYV
                     MDEANVESHYLWQYRNQSPVLYPEWKDAIVDRGVSMVMRDRNHPSVIIWSLGNESGDG
                     PNMVAMADTIRKIDAAKRPIHYESKALKRPLSFDGVGAFEKLRRMLSALQWSKALTEY
                     DFNAGMYPTLDRLKEMAKLDKKERPILICEYSHAMGNSNGHFKEYWDLFESHPRMVGG
                     YIWDWTDQGLIKHTSNGKPFYAYGGDFGDKPNDKDFCLNGVTFPDRSLKPAMAEIKKV
                     QQYIKFTGIDFDRKTVNLKNTYAFLNLKSFNLTWSISEDGITVQKGELPLPDVKPNGE
                     GSVTIPYTLPQINPGKRYHLNLSVQLANDEPWAKKGFEVATYQYEMPWLVQKPTTIAA
                     TSGNLNVTENADTWIVSGQAFSIEFNKTTGTISKWKSNGEMVAQNGPRVNLWRAPTSN
                     DIGTGFNPDPRFSFHGKLWQKYGLDNLIIKKSKVTISTTGKGVVVLTHQTLEGNKSRF
                     NAVITHVINPDGVVDINYKLTCSKKFNLPRVGLMLELPKEFSQVEWLGRGPQENYRDR
                     SYAAHWGLYRKTVDSMVTPYIKPQENGNRYDVDRVSIFNPNGTSGIEVTGKSFCFSIH
                     PYSLETLTKATHTPDLVESPVNHLYIDLAQNALGSESFFYNYLQEYVLKGKKFEFQVS
                     LKPVTKN"
     gene            13148..15112
                     /locus_tag="PK049_07445"
     CDS             13148..15112
                     /locus_tag="PK049_07445"
                     /inference="COORDINATES: protein motif:HMM:NF025861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HOK61430.1"
                     /translation="MKRISILTALIFTTDLLFGQSYTLKSPNGILVANIDLKDRLTYS
                     LSCNGNAIVEASGIAMRTSAFAESDFLGKHTATFNSKNQAVKLLWGTTKEIDENFNQL
                     KLNYSNGLSVEFRCYNEGFAWRVVSAFDREVIVYNETSEFNIPNSATVYFPEVKGFLT
                     PFEVNYLPKSMQTIADGTLGLTPFMYKTPSGELVVISESNLFEYPGMFIRKDAGNSVK
                     AVFPLYPKKEKQELPGRLHLVKWPQISRMVVKKTENYIAKSNGNRSYPWRVVMVAQND
                     AEILQNSLVYLLADEPKMDYGWVKPGKVVWDWYHNWNLQGVEFKPGINTQTYKYMVDF
                     AANNGIEYINIDDGWAKLWDFKKVNPNLNLKEVIDYANSKGVKVFIWAMWNTIDKDFT
                     ANLDMFSKLGVAGIKVDFFDRADQRIVDFVNKLADECAKRNLLLNLHGMYKPTGINRT
                     FPNIVNIEGVLGLEYNKFSDKCTPVHNLTIPFVRNVVGPMDYTPGSMRHVTPEQFVKS
                     WENPHSMTTRAQQMAMYIVYHGGVQMLADSPTLYQKDSIAQNFLSGVPVTWDKTIALE
                     GKVGESIAVARKHGDKWYIGCMTANQEHDFNFNFNLNLNLLDDREYKMTIIAEGNSDD
                     ELVLSSKRIGKNDRQNFKVKPRSGLVIILE"
     gene            15117..16268
                     /gene="galK"
                     /locus_tag="PK049_07450"
     CDS             15117..16268
                     /gene="galK"
                     /locus_tag="PK049_07450"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HOK61431.1"
                     /translation="MVERIVSTFKDRFGSDPFVFRAPGRVNLIGEHTDYNNGFVMPAA
                     INRYAYFAIKPNGLGLYRFHAADFRRDFTTPVYKIEPSNAHWTNYLLGVIAQMVAEGK
                     DIPGFDCVFGGDVPIGAGMSSSAAIESGLAFALNHIYGLGFTRMQLAQIAQRAEHEYA
                     GVKCGIMDQFASLHGKQNHVLLLDCRSLEFEPFELNMDNHSIILVNTGVKHSLASSEY
                     NKRRQECQAGVDILKKYNNSIQSLRDVTINDLNNHKREFSSKVWERCLYVVEENQRVV
                     NAGKALTQKDFESFGKLMYQSHYGLRDMYEVSCPELDKLVELTEKLDYVVGSRMMGGG
                     FGGCTINVVKNAHIDEFTKCVKENYKTPDGKIPQIITCVISNGAELLVY"
     gene            complement(16334..17254)
                     /locus_tag="PK049_07455"
     CDS             complement(16334..17254)
                     /locus_tag="PK049_07455"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOK61432.1"
                     /translation="MKISVVLPCYNEEGNIKLLYSELRSVLTDKYEYEIIFVDDGSVD
                     GTLSTIKEIAMDDRKVKGISLSRNFGHQSAIIAGMNSASGDYIITMDADMQHPPTLIP
                     TLINKANEGYDIVLTIRKSTVDAKFIKRITSHLFYRFFNYLSDIPLKEGSADFRLMTR
                     NAANVFLGLPEKVRFNRGLINWMGFKTAFVEYHSPRRISGKSKYTFIRMIKFAIAGIL
                     SFSSKPLKIPFILGLLSFIFGLGYSIYVLVQFINGMTIPGWAFTIILILMLGGAQMLS
                     LGIVGLYIARIFEETKQRPIYIIKERINIE"
     gene            complement(17251..18843)
                     /locus_tag="PK049_07460"
     CDS             complement(17251..18843)
                     /locus_tag="PK049_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61433.1"
                     /translation="MIRFLKDINLRLKLLLVLIVLTFLYGFYIDFFEGPKPIHEWRKA
                     DCLSITYNYMKGEPFFEPKTNLILENGNRNAVSEFPIIYFLMGKLWSVIGQNEFLYRL
                     LSFVLLLLGIILFATVVQYYLKSQKYTIIYSVFMFSSPILIGYAHNFIPNPISFALNL
                     IAGFFVFQFLQNNKLYGLIGFFVFSTLAILIKITSILVFMSFIGAFIFYELFDNNYFW
                     RKYKIKISLLSGSFILTLALVAAWYYYAIYYNTKYDSHLFSTTIRPIWEIPAEKRNEI
                     FWHIINTMINYAYHRVIVFALIIFGVVALFSRSINAFLKWLIVMSIGALFSYLILWYW
                     VFDVHDYYLIEILFVFNVLFFIFLYKINSFFSFLDLKKINLALYFFIIFVVLHAYTCS
                     WVRFSANPKLIVNNIFLKKRDIDTWRWFNYDYGINLKTLQSKSDEIKKIIQPSDTILC
                     LRDYSPNVHLYTLGRIGYSKFLYNGFSDREAIVDAYKRGARYVVFLGSEEERAKIPYS
                     DYVAYQYERIYIFDLKPYKKIQ"
     gene            19076..19582
                     /locus_tag="PK049_07465"
     CDS             19076..19582
                     /locus_tag="PK049_07465"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HOK61434.1"
                     /translation="MIDVKLSIVNSKPEIFRIIRVDENINLQLFHEVIQAAFEWSNMH
                     SHFYNADKSILKNEEEILLGNALNKWESLIYVYDLADFWTISISLLATNSGELSKKPV
                     CLQGQGKSPPEDSGGIINYCEVLELLAKHNKGDRQYPLIADWLGENYNPNEFNCEQVN
                     LNFKNLVL"
     gene            19587..20093
                     /locus_tag="PK049_07470"
     CDS             19587..20093
                     /locus_tag="PK049_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HOK61435.1"
                     /translation="MEYRGDPLVYQTLNELSIPFEYYEHPEAPTVEIASKYWEGINSS
                     HCKNLFFRNHKGNRHYLVILEYRKMLNIHQLEKLLKQGKLTFASSERMKRYLGLTPGS
                     VSPFGLLHDTSRHVHLFIDSELKNTQRLSFHPNLNTASLVIPTIDFFRYLNWVGNTYE
                     FIDYVNEL"
BASE COUNT         6407 a         4309 c         4056 g         5494 t
ORIGIN      
        1 tttgcccaaa aggcaaagcg ggttgttacc gtaagccaat tttcacgtac cgatattgcc
       61 aataatttta atatcgaccc aaataaaatt gatgtagcat acaacggtgc aagcaacagt
      121 tacaagcctc tttccgaaag tgaaattcag cgggtacggg agagtatctc cgagggtaaa
      181 ccctactttc tttttgtggg agcatttaat ccgcgcaaga atgttgcccg tttaattgaa
      241 gcctacgaca tattccgcaa aaggactggt tcagcaataa acctcctgct tgtaggtgaa
      301 cccatgttta aaaccaacga tatcggcata gcctataaaa actgtaacca caaaaacgat
      361 atcaaattcc ttggcaggaa aggccttgat gaactcacta aaattactgg ggctgcacat
      421 gctgtaattt acccttcgac ctttgagggc tttgggattc ccttgctgga ggccatgaaa
      481 tgtggcatac ccatagcagc ttcaaacgtc acggctattc ctgaggttac gggtaattca
      541 gctatttact tcgaccccta ttccgtggat gagatggctg atgccatgca gcgtgtagcc
      601 tacgatactg aactgcgaga aacacttagc gccaacgcgc tgaaccgaca acaacaattc
      661 agctgggata atactgctca ggtattgtac agtagcatcg aaaaatgttt ttagcaatgc
      721 ttaagcagta cctaaatgtc aatgtgattg aagccggatg cgatgaagcc ggaaggggat
      781 gcctggcagg ccctgttttt gcagccgctg ttattcttcc acccgacttc aacgacccgc
      841 tcattaacga ctccaagcag ctaaaccaca aacagcgcat gcagctccgc aaggttattg
      901 agcactacgc attggattgg gcagtagcaa gtgtatcgaa ccaagagatt gacaaaataa
      961 acatactgaa cgcagccata catgcaatgc acatcgctat tggaaaactt agcaaactga
     1021 aaccagaaca tttgctgatt gacggtaacc gttttaaacc ttatcccgga attccccata
     1081 catgcattat caagggcgac tctaaatttc tttccatagc cgcagcatcg atacttgcaa
     1141 aaacttaccg cgatgagttc atggtgaaca ttagcaagga gtacccgctt tataactggg
     1201 atatcaacaa gggctatcct acaaaaaagc atagggagag tattgccaaa catgggacta
     1261 ctccttacca ccggctaagt tttaggctat tgtagctata ttgtgtgaag ttgaattaaa
     1321 ctaataccat atttagttgt ccaaaaaaac aaacctaaaa aataaatgcc atgattaaac
     1381 gattaacaac acttttactg ggatttttgc tcctggttag ttcagccatt aaagccgacg
     1441 aggggatgtg gcttctccct cttctgcaaa agtataacat ccaagccatg cagcagaaag
     1501 gctttaagct tactgccgac gatatctata acctcaacca ggcaagtata aaggatgctg
     1561 ttattatttt tgggcgcgga tgcaccggcg aggtggtttc cgaccagggt ctggttctca
     1621 ctaaccacca ctgtggttac ggcgccattc aacagcacag caccccggaa cacgattacc
     1681 ttgaggatgg tttctgggct aagtcgtttg aggaggaaat acctacaccc ggcttaacag
     1741 caactttcct tattcgtatt gaggatgtta ccgaccaggt taacgctgcc tttaaacctg
     1801 aaatgtccga agctgatcgc gacaaggctg ccatggagat tggccgtaaa attgcaaagc
     1861 aagcaaccga tggcacccac tacaccgcaa gtgtacgcag tttctttggg ggaaaccagt
     1921 atttccttct ggtttatgag gtattcaacg atgttcgact ggttggagca cccccatcat
     1981 caataggtaa gtttggtcac gacaccgaca actggatgtg gccccgccac accggcgatt
     2041 tctcggtatt ccgcatttat gccagtaagg ataacaaacc tgccgactac tcgcgcgaaa
     2101 acgttcccta caaacccaag catttcctac caatatcgct gaagggggta aacaatggcg
     2161 atttcaccat gataatggga tacccgggca gcacacaacg ctacatgacc tctgtagagg
     2221 ttcaggaacg tattaatata ctcaacgcca acaggatata cgttagaggt attaagcagg
     2281 aaatctggct aaaggatatg caagccgaca aggccgttaa cattaagtac gcctcaaagt
     2341 acgccggatc cagcaactac tggaaaaact ccattggcat gaacaaagcg ctaaagcgcc
     2401 ttaaggttta cgatttcaag aaacagcagg aagaggagtt taccaagtgg gttcttgcct
     2461 cgccagaacg tcaacagaag tatggcgaag cacttagctt gattaatgag ggtgtaacag
     2521 gccggaagga gtacctacac gccagccagt acattagcga agcactattc agcggaactg
     2581 aaataattgg aatggcagcc atgacccgtt cattggttga tgctctaaag ggcaacaaaa
     2641 ccgaagaggt taccaaaatc agtaagtcgc taaaggctcg ctccgagaac ttctacaaga
     2701 actacaacac accaaccgac caaaaaacgg ccaaggccat gttccgcatt ttcatggaaa
     2761 atgttccgct aacctatcaa cccgatatct tcaacaccat cagaacaaag tacaagggcg
     2821 atgttgacaa gttcgttgac cagatgtttg ccaaaaccat gtttactgat ccagctaagt
     2881 tcaacgcttt ccttgacaag ccaagcctaa aggttcttga aaaagaccct gtgtacgttg
     2941 cagcggcttc catctcaaac aaatacgaag aaattagcaa agtaatggca ccattcaacg
     3001 aaaagttgca acgcggacag cgcctttaca tagctggggt gcttgaaatg aacgaaggca
     3061 aagccatgta ccccgatgcc aatttcacca tgcgcctcac ctatggtgaa ataaaagact
     3121 attaccccat ggatgcagtt cactacgact acctaaccac ccttgatgga gttatggaga
     3181 aggaagatcc ggacaactgg gaatttgttg ttcctgccaa actcaaggaa ctatataact
     3241 caaaggatta cggccgttac gggaaagatg gtaaaatgcc agtggcattt atatcaacca
     3301 atgatatcac tggcggtaac tcaggaagcc ctgtaatgaa cggcaatgga gaacttattg
     3361 gcattgcttt cgatggaaac tgggaagcta tgagcggcga cattgttttt gaaaacaagc
     3421 tgcagcgcac catatcggtt gatatccgct acgtgctatt tgtaattgat aagtatgcag
     3481 gtgcaacccg actgattgac gaaatgaaaa ttattgagta gtgtacctaa caaacataaa
     3541 aaaagccgag tattaactcg gcttttttnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnntg gtacctgggt taaaatgaaa cgccgtaaat cttcaatttt cttattttta
     3721 ccacgactca gttttttttc atcaattcaa tatatactat tcgtcaaaat gtattacctt
     3781 tagtcggcta attagtgaaa ctttgcgtta aatatcatag taattaatta tacaaacacc
     3841 tcaaactatc gtggaaaaag gcgaagtttt aataaatcat tacggtccac tctcctatga
     3901 ggagataggt tttttgctca acaagatgac atcgttgctg gagcggtaca actttggaat
     3961 taccattcgc aaaaaggttt actcggcaat ggtcgagagc ctggaaaaca tatataagca
     4021 tcaggatgtt ataaagggga gcaataacta tcaaccaaaa ttttcgctag tactggaaaa
     4081 agacaaggta gacttatcct gctcaaactc attactaaaa gtaaaaattg gaccgttaaa
     4141 agaacgattg gaaaaagtta atcaactcga taaagctggc ctaaaggagt tttataagga
     4201 ggttatcctc agtggaaatg tcacccagaa aggtggtgct ggtctgggca ttattaatat
     4261 cgccaaggtt actgaaaaca agctggagtt tacttttgac gatattgatg aacagtacac
     4321 atactttact ctgagaatta aggtgtcgct tcaaaaccaa aacaactagt gaccatggaa
     4381 ccattataca tagagcccag cgagtttaca ccccgcgtgt ttttcgatcc ggaaaattcg
     4441 gttttcgaga tctcaggttt ttcaaggccc gaaaataccg ttggattcta caggccactc
     4501 atcaaatggc tggaagacta cgaggataat gttttggcaa aaaacactaa ctttaacaaa
     4561 agcaaccttg tactgaatct caagcttacc tacttcaact cagcctcatc caaattcctt
     4621 atggatatac ttatggtttt catgaagttt cactccaaag gcaatgccgt tgaaattaac
     4681 tggtattacg atgaggatga cgacgaaatt tttgagtcag gcgaggaaat ttcagagatg
     4741 attaatttcc cattcaactt tattcctatc acacctaact cgtaaaattg attagaatga
     4801 acccattagt tttacaggct agcaacacaa caccatatat cgattttaac ccaagcgaga
     4861 atagactgac catttcaggc tactcacgcc ctgagaatgt tagagacttt tatatgcccg
     4921 ttatggattg gttggaatcg tttagcaacg agctaaagag caagatgtta aaggaagaac
     4981 aaatccaacc cattgaattt cagtttaaac tggtttactt taactcatca tccgcaaaat
     5041 tcctatacga cattatcatc atacttaacc agatacaaaa agctaaggta ccgttaaaca
     5101 tcaactggta ttacgacaag gatgattccg aacttcgtga ggccggtgag gatttatcgg
     5161 aactggctca ggttccattc aacttcgtta cctactagta attccaaatt tccataattg
     5221 ccaaggtgct gaattggaaa tgtatccaat tccattcaac ttttttggta aactttcatg
     5281 aaacctgtta taaatcaata cctttagcgg gtttgctgaa ggaaagtgaa atacccttta
     5341 aaaaaagtgt gctttaaaaa atctatttgt taacaaaatt ctatttttgt tggttctgat
     5401 aaaacactaa aagcaatgac cgaacacgaa ttaaccctag gggacagcgg cgaaaggcca
     5461 aaaaattttt tagaggagat tattgaggaa gatattcgaa caggcaaaca tggaggccga
     5521 gtgcttaccc gtttcccgcc cgaacctaac ggatatctgc acattggtca tgcaaaatcg
     5581 atatgcctta actttggttt ggctcaaaag tatggtggca aaaccaacct gcgtttcgac
     5641 gataccaacc ctgtaaagga ggacgtagag tatgtaaact ccataaagga ggatatacgt
     5701 tggcttggtt ttgaatgggc aaatgagttt tacgcctccg actactttga gcagctatac
     5761 gagtgggccg agctgctaat caaaaagggg ttagcctacg ttgatgacca gtcgcaggaa
     5821 gagatacgca taaaccgtgg tactgtaacc caacccggaa aggaaagtcc ttggcgcaac
     5881 cgatcggttg aggagaacct cgaccttttc cgccgcatgc gcgccggcga gtttaaggat
     5941 ggcgaaaagg tgctacgcgc taaaattgac atggcgcacc ccaatatgct attacgcgac
     6001 cccattatgt accgcattat acatgctgag catcaccgaa ctggcgacaa gtggtgcatt
     6061 taccccatgt acgattacgc acatggccaa tccgattcca ttgaacaaat tacccactcc
     6121 atttgtacgc tagagttcga tgtgcatcgc cccctatacg attggtttat tgagaagctg
     6181 gagattttcc cttcaaaaca gtacgagttt gcccgcctga acctctccta tacagtaatg
     6241 agcaagcgta agcttttaca gctggtacag gaaaaggtag taatgggctg ggacgatccc
     6301 cgtatgccta ccatttgcgg tttacgtcgc aggggttata ccccagaagc catacgttac
     6361 tttgccgata aggtaggtgt ggccaaacgc gataacgtaa tcgatttatc gttactggaa
     6421 ttttgtatcc gcgaggattt aaataagcgt gctgagcgcc gcatggctgt actcaacccc
     6481 cttaaggtgg ttattaccaa ctaccccgaa ggacaggttg aagagcttga agccgtcaat
     6541 aaccccgagg acgaaacagc tggtaagcgc cttataccat ttagccgtga aatatacatt
     6601 gagcgcgacg acttcatgga gaacccaccc aagaagtttt tccgtctggc tcccggtcag
     6661 gaagtgcgct tgcgctatgc ctacttcata aagtgcgagg aggtaattaa ggattcccaa
     6721 ggcaatgtta ttgagttaca ttgcacctac gacccagcct cacgcggcgg taactcgccc
     6781 gatggacgta aggttcaagg cacaatacat tgggtttcca ttccgcatgc cgtaaaagcc
     6841 gaaatcaggc tattcgatag gctattctcc aaacccgacc ccgacgatgt tcccgaaggg
     6901 caggattgga aatcgtcact caatcccgaa tcgctcacag ttattgaggg gtatctcgaa
     6961 ccatcactaa aacacgcaaa acaattagat aagttccagt ttgagcgcct tggttacttc
     7021 tgtgttgatt acgattcaac gcctgaacat ctggtgttca acagaactgt aactctgaaa
     7081 gacacttggg caaaattagc cggcaaatag ccggctattg cccttaacaa aaagccttac
     7141 cataaaaatt tgcaaaaata ttttgcaaat caatttttaa aaacattatc tttgcagcgt
     7201 tgtaacagca ttgtcctgtg gtgtaattgg caacacgtct gactctggat cagaagagtc
     7261 caggttcgac ccctggcagg acaacaaaaa accgctcagc tgagcggttt ttttattttc
     7321 ttttttctac atttatgcaa aattattgcg atgaccaacg taatacccat ttaccatatt
     7381 ggccttattc aggcctggtt tgcagcctta ctggtttacc gaaaaccaaa caagggagca
     7441 gccgaccggt tactggttat ttggctaatt gtgattggta tcgagatgct ttattcccta
     7501 cttaatgcaa aatatctaac ctggctgccc gatttaatta ttatcccgct aagctatggg
     7561 cctttcctgt tcttctatac taaactttta acatcaaaaa agcctacaac cctccggaat
     7621 attcataggc acctcattcc gttttacctt ttcatactta ttgcagccgt ttggggcaaa
     7681 ccaattgata ttgactccgt taacttttac agcaaagggc caatcacttg gctggcaatt
     7741 ttcaactatg ctcttttcct gatagttatt ctttactact ggcgagcatc gctcattcta
     7801 atccgaaaac ataatcgcac actgggcgac catttctcgt accgctcgga aactatcatg
     7861 ctaaactggg tcaaaaatat agccatttgg atcctctctg gcttttttgc ttcgggcatt
     7921 acctttataa ttttttcaac ccaaaatatt ttccctttta atcccattga gcttttccat
     7981 ttgggtttac tggttttcac cttttccata agctattacg ggatccacca gccggtactc
     8041 taccaatacc aaaaacctaa acaaatagag gtaactgaag atttacagac caaaacatca
     8101 caaaaattga gcgatttact ttatgagcac atggttaaga acaagcccta cctgaataat
     8161 gagttaacca ttcaggattt agccgaccag ctcaaagtaa gttcccagga aatctccagt
     8221 taccttaaca aggaacttgg cgtaaacttt ttcaactata tcaacggttt caggattgaa
     8281 gaggccaagc aaaggcttac aaatcctaac ttcgacaacg agaccctcct gggtattgcc
     8341 tacgattcag ggtttaactc caaaacatcg ttcaataccc tttttaaaaa ggaaaccgga
     8401 atgacaccat ccgaatataa aaaggccttt aggagttcaa ctaaaaaaaa tacttagaag
     8461 aatctcctca atcatcctgt gttataaaat ctgtatatca attagttaga acgtgtaact
     8521 tttgataaaa ttgttcaaac tttttatttg aacgaccagg ttacatcact tttctaaata
     8581 gctatccaaa accttaacca aaaatcaaaa tttaaagcta tgagaaaagt tatttttgca
     8641 ttactgattt gcatggttgc aatgccatca ttcgcacagg aaaatcagga aaagaagacc
     8701 aacgaagaag taaaaaccgg ctggacagtt ggtgttttac cggcattagg ctacgactca
     8761 aacctgggct tcctttatgg agccattgtt aactcgttcg attttggcga cggctccagg
     8821 tatcccgact tcaaccacaa cctttacctt cagctatcgg cctatacaaa gggtagcatg
     8881 gatgccattg cctacttcga ttcctacaca cttattccaa acaaacactt tattggtcgc
     8941 ctaagcttta accggaacag gacataccca ttctacggtt ttaacggcac acaaaccgtt
     9001 tacaacgccg attttgaaaa tatcaatgcc accgacgatt ttatttctca agtattttac
     9061 aaatacgacc gtaagcttgc aaaagccgat ttcctacttg aagacagaat agccgacagc
     9121 aaatttaagt ggattgcagg ttttgacctt ggctggtacg atgttgcaac agtagatatt
     9181 gaccacctaa acaaaggtcg cgacgacgat gagcttatcc ccgatgttcc cattctatac
     9241 gatcagcttg ttagctgggg aatcatctcg gccgacgaaa aagatggtgg atttgataat
     9301 agtgttaagt ttgggctggt ttttgatacc cgcgaccgct taactaaccc aatgaaaggg
     9361 gtatggacag aactactttt tagggcatcg cccaagttca ttggcaacaa cgataactac
     9421 ggacgagtat cgttaatcca tcgccaatac ctaacgcttg taaaggaaaa actatcgttt
     9481 gcatatagac tctggtacga agggaaccta ggtaacgtgc ctttttacgc ccgtcagcat
     9541 ctaaccacca gcaactacta cgaaggtttt ggtggcgctc aaaccgttag gggtgttttg
     9601 atgaacaggg ttgttggtca gcatacagct atcggtaatt ttgagcttcg ttggaaggca
     9661 taccgcttcc atgccattgg gcaaaacttt tacctaggct ttaacctttt tgccgatgct
     9721 ggcaaggtat tgaaaggtta cgatatggac ctaacaaatg ttcctgttgc ccaacgcaac
     9781 cagctgtttt caaacagcta taaagattta tataccacat ttggcgctgg tttgaaaatc
     9841 gtaatgaatg aaaactttgt ggtatcggcc gattatggcg tggccatgga caaaaattac
     9901 ggttcatcgg gattgtacgt tttggttggt tatctgttct agattcaaga aaaaattgct
     9961 aaatgatgat gcgaaaagca cttttcatat tactctgcgt ttgcagcact atgctgcaag
    10021 cgcagaactg gcaggaccca aaggtttttg ccataaacag gctgccctca cacactctac
    10081 tggtcccatt tcctgaaaag tcaagcagta tttcctacat tttcaattct gaatttgtgc
    10141 aatccttaaa tggggattgg aaatttatgc tgataaacaa cccgttgaac actcccgaaa
    10201 acttttcaaa tgtagaattt accgatagcg aatggagcac cattcctgtt ccatcaaact
    10261 ggcaaactaa ggggtatggt actccaattt actcaaacca aatacaccca ttcccagcta
    10321 atccaccctt tgttccaaac gagggaaacg agaccgggct ttaccgccac accttccgga
    10381 tccctgagaa ttggaatagc agtagggtaa taattcactt tgctggggta caatcggcaa
    10441 ttgacctttg gataaatggc cattacgttg gctactcaca aggaagcatg acccccgccg
    10501 agttcgacat tacaccatac attaccgctg gacagaatct tttggcagca agggtaatca
    10561 actggtccga tggaagttac ctggaagatc aggatttctg gcgattaggc ggaatttttc
    10621 gcgatgtatt tgtttacgct cagcctcaaa atgcactctg ggatattgag gttaacactt
    10681 cattcagtaa aagctatact gaggccaccc tttcagttag cggggtacta acaaatacaa
    10741 cgagccagct acccaacatc agcctttcac tttttgatag taatggcatt gcagtgtttg
    10801 aaaaaacggt tcaacccaaa ctgagcagtg ggatagctac ctttaaagta gatgagatag
    10861 tgaacaaccc acagctttgg agtgccgaga cgcctaatct ttatcaactt attgttgaga
    10921 ttgagggtag atactaccag cagctggttg ggttcaggga tgttaaagta gaaaacggac
    10981 agctactagt taacggacaa ccaataaaaa taaagggagt aaaccgacat gagttcgacc
    11041 cctacaacgc tcgtacggtt tcgtatgagc tcatggagaa ggatgttgtc ctgatgaaac
    11101 agaacaactt caatgcagtt cgtacagccc actatccgca tcatccatat ttttatgagc
    11161 tatgcgacag gtatgggctc tacgtaatgg acgaggccaa tgttgaatcg cactacctat
    11221 ggcagtaccg taatcaatcg ccagtgcttt accccgaatg gaaggatgct attgttgacc
    11281 ggggggttag catggtaatg cgtgaccgca accatccatc ggtaataata tggtcgctag
    11341 gtaacgagtc gggcgacgga ccaaacatgg ttgccatggc cgataccatc agaaaaattg
    11401 atgctgcaaa aaggcctatt cattatgaaa gcaaagctct taaacgcccc cttagcttcg
    11461 atggtgtggg tgcctttgaa aagttaaggc gaatgttatc ggccttacaa tggtcaaaag
    11521 cccttacaga gtacgacttt aatgctggta tgtaccctac cctggatagg ctgaaggaga
    11581 tggctaaact cgacaaaaag gaacgcccta tacttatatg tgagtactcg cacgccatgg
    11641 gcaacagcaa tggccatttc aaggagtact gggacttatt cgagagccac ccacgaatgg
    11701 ttgggggcta tatatgggac tggactgacc aaggtctgat taaacataca tctaacggca
    11761 aaccattcta cgcctacgga ggcgattttg gcgacaaacc caacgataag gacttttgcc
    11821 tgaacggcgt tacttttccc gaccgttccc ttaaacctgc tatggccgaa ataaaaaagg
    11881 tacaacagta cattaaattt acagggattg attttgatcg caaaaccgtt aaccttaaga
    11941 atacatatgc cttcctgaac ctaaaaagct ttaacctaac atggagcatt tctgaagacg
    12001 gcattaccgt tcaaaaaggg gaactgccgt tacccgacgt taagccaaat ggagagggct
    12061 cggtaacaat tccatacacg ctccctcaaa tcaatccagg caaacgttac catcttaacc
    12121 tatcggttca actggcaaac gatgagccat gggctaaaaa gggctttgag gtggcaacat
    12181 accagtacga aatgccctgg ctggtacaaa aacccaccac cattgctgcc actagcggta
    12241 atttgaatgt aaccgaaaat gcagatacgt ggattgttag tggccaggcc tttagcattg
    12301 aattcaataa aactaccgga acaatttcaa aatggaaatc gaatggcgaa atggtagccc
    12361 agaacgggcc aagggttaac ctttggcgcg cccctacaag taacgatatt ggcacgggat
    12421 tcaaccccga ccctcgtttc tcgttccacg gaaaactttg gcaaaagtat gggttagaca
    12481 atttgattat taagaaatcg aaggttacaa tttccacaac cggcaaggga gttgtagtgc
    12541 taacccacca gacccttgaa ggtaataaat cgagatttaa tgctgtgatt acccatgtta
    12601 ttaaccctga cggagttgta gatatcaact ataaattgac ctgtagcaaa aaattcaacc
    12661 tgccccgtgt tggccttatg cttgagttgc ctaaagagtt ctcgcaggtt gagtggcttg
    12721 gccgcgggcc tcaggaaaac taccgcgacc gcagctacgc tgcacattgg ggattatacc
    12781 gcaaaaccgt tgatagtatg gttacaccat acataaagcc tcaggaaaac ggtaaccgct
    12841 acgatgtgga cagagtaagc atatttaacc ccaacggtac aagcgggatt gaggtaaccg
    12901 gaaaatcgtt ctgctttagc atacatcctt actcgttaga aacactcacc aaggcaacac
    12961 atacacccga cctcgttgaa agtcccgtca accatcttta tatcgacctg gcgcaaaatg
    13021 cacttggtag cgagagtttc ttctacaact acctgcaaga atatgtttta aagggcaaga
    13081 agtttgagtt tcaggtaagc ctcaaaccgg ttaccaaaaa ctagttaaaa tgcataatgg
    13141 caacacaatg aaaagaatat cgatactaac agctctgata tttactacag atttgctgtt
    13201 tgggcaaagt tacaccttaa aatcgcccaa tggcatttta gtggcaaata tcgatttaaa
    13261 ggataggtta acctactcgc tatcctgtaa tggcaatgca attgttgaag cctcgggtat
    13321 agcaatgaga acgagtgctt ttgcagagag cgattttttg gggaagcata ccgcaacatt
    13381 caatagcaaa aaccaagctg tgaaattgct ttggggaact acaaaggaga ttgatgaaaa
    13441 tttcaaccag ctaaagctga attatagtaa tggcctttcg gttgagttcc gttgctataa
    13501 cgagggtttt gcttggcgtg ttgtttcagc atttgacagg gaggttatag tttataacga
    13561 aacaagtgaa ttcaacattc ccaacagtgc aactgtttac ttccccgagg taaagggttt
    13621 ccttactcca tttgaggtta attacttacc aaaatcgatg caaaccatag ccgatggcac
    13681 tctaggttta acccccttca tgtataaaac accaagcggc gaactggttg tgatatcgga
    13741 atcgaatctc ttcgaatatc cgggcatgtt catccgcaag gatgcaggta actcagtaaa
    13801 agcggtattc cctctttatc ctaagaagga aaaacaggaa ttgccaggca gacttcatct
    13861 ggtcaagtgg cctcaaattt caaggatggt ggtaaagaaa accgaaaact atattgccaa
    13921 aagtaatggt aatcgttcat acccctggcg tgtagtaatg gtggcacaaa acgatgccga
    13981 aattctacaa aatagcttgg tttacctgct tgccgatgag cccaagatgg actatggttg
    14041 ggtaaaacca ggtaaggtgg tttgggactg gtaccataac tggaacctac aaggcgttga
    14101 atttaaaccg gggataaata cccagaccta taagtatatg gttgactttg ccgccaataa
    14161 cggcattgag tacatcaata ttgacgacgg atgggctaag ctttgggatt ttaaaaaggt
    14221 taatccaaac ctgaacctca aggaggtcat tgactatgcc aacagcaagg gtgttaaggt
    14281 atttatttgg gcaatgtgga acactattga taaagacttt acagctaacc tcgacatgtt
    14341 tagcaaattg ggagtagcag gaatcaaggt agactttttc gaccgcgctg accagcgtat
    14401 agttgatttt gtgaataagt tagctgatga atgtgccaag cgcaaccttt tactaaacct
    14461 acacggcatg tacaagccta ccggaataaa tcgcactttc ccaaacattg ttaatattga
    14521 gggagtgctt gggcttgagt acaataaatt ttccgacaag tgcacaccgg ttcataacct
    14581 caccattccc ttcgtaagga atgtggttgg ccccatggat tacactcccg gctcaatgcg
    14641 acatgtaacc cctgaacaat tcgtaaaatc atgggagaac ccccactcca tgactaccag
    14701 ggcccagcaa atggcaatgt atatagttta ccacggcggg gttcaaatgc tagccgactc
    14761 gccaacgctc taccaaaaag atagcattgc ccagaacttt ttatcaggag ttcccgtaac
    14821 atgggataaa accattgccc ttgaaggcaa agttggcgaa agcattgcag ttgcccgtaa
    14881 acatggtgat aagtggtata ttggatgtat gactgcaaac caagagcatg actttaactt
    14941 taactttaac ttaaacctca acctcctcga tgacagggag tataaaatga caattattgc
    15001 tgaaggtaat tccgatgacg aattagtact aagctcaaaa cgtattggta aaaacgatag
    15061 acaaaacttt aaggtaaaac cccgtagtgg attggtaata attctagagt aaaccaatgg
    15121 tagaaaggat tgtttcaact tttaaggatc gttttggctc cgatcccttc gtttttagag
    15181 ctcccggcag ggtgaatctg ataggcgaac acaccgacta caataatggt tttgtgatgc
    15241 ctgcggccat aaaccgttac gcatactttg ccatcaaacc aaacggactt ggcttatacc
    15301 ggttccacgc tgccgatttt aggcgcgact tcacaacccc cgtttacaag attgagccat
    15361 caaatgcaca ctggacaaac tacctattag gggtaattgc ccaaatggta gccgagggta
    15421 aagatatccc aggttttgat tgtgtttttg gtggcgatgt tccaattgga gcgggaatgt
    15481 catcgtcggc agcaattgag agcggattgg cctttgcgct gaaccatatc tatgggcttg
    15541 gttttacgcg catgcaactt gcccaaatag cccaaagggc tgagcatgag tatgccgggg
    15601 taaaatgcgg tataatggat cagtttgcat cgctgcatgg caagcaaaac catgtactgc
    15661 tgctcgactg ccgtagcctt gagtttgagc cctttgaact caatatggat aaccattcca
    15721 tcattttggt taataccggg gtaaagcact cgctagcttc gtcggagtac aataaacgca
    15781 ggcaggagtg ccaagccgga gttgatatcc ttaaaaagta caataattca atacaatcgc
    15841 tacgcgatgt aacaatcaat gatttaaaca accataaaag agagtttagc agtaaggtgt
    15901 gggaaaggtg tttgtatgtg gttgaggaaa atcagcgcgt ggttaatgct ggtaaagcac
    15961 taacccaaaa ggactttgaa tcgttcggaa agttaatgta ccagtcgcat tacggactcc
    16021 gtgacatgta tgaggttagc tgtccagagc ttgacaagct cgttgagtta actgaaaaac
    16081 tcgattatgt ggttggctca cgaatgatgg gcgggggttt tgggggttgc acaataaatg
    16141 tggttaaaaa cgcccatatt gatgagttta caaaatgtgt aaaagaaaac tacaaaacac
    16201 ccgatggtaa aattcctcaa attattacct gtgtgattag taacggagca gagcttttgg
    16261 tatactaaag gttaggtcag ctttactccc acttaaaggc tatccttttt cggatagcct
    16321 ttttgttttt taattattca atgttaattc gctctttaat gatataaata ggacgttgct
    16381 tggtttcttc aaatattctt gctatgtaaa gcccaactat tccaagactt aacatctgag
    16441 cgccacctag catgagtata agaattatag taaaagccca tcctgggata gtcatgccat
    16501 taataaattg aactaaaaca tatattgagt aacccaatcc aaaaatgaat gataaaagcc
    16561 ctaatataaa gggaattttt aaaggtttag atgagaaaga aagtatacca gcaattgcaa
    16621 actttatcat ccgaataaat gtatatttag attttccaga aatgcgcctg ggagaatgat
    16681 actcaacaaa agctgtttta aaacccatcc aattgattaa accccgatta aaacgtactt
    16741 tttcaggcaa acctaagaaa acattagcgg catttcgtgt catcaagcga aaatcggcac
    16801 tcccttcttt taatggaata tcagagagat aattaaaaaa acgatagaat aaatgcgatg
    16861 ttattctttt aataaatttt gcatcaacag tactttttct aattgtaagt acaatatcat
    16921 aaccttcatt tgctttgtta attaatgttg ggattaaagt tggcgggtgc tgcatatctg
    16981 catccatagt aattatatag tcgcccgaag cactattcat cccagcaata attgcagatt
    17041 gatgcccaaa gtttcttgaa agagatatac cttttacctt tctatcatcc attgcaattt
    17101 ctttaattgt tgagagagtg ccatccacac tcccatcatc aacaaaaatg atttcatact
    17161 catacttatc tgtaagaaca ctcctcaatt cactatatag taattttata ttaccttcct
    17221 cgttataaca aggcagtact acacttattt tcattgtatt ttcttatagg gtttaagatc
    17281 aaaaatataa attctttcgt attggtaggc aacataatct gaataaggta ttttagcccg
    17341 ctcctcctca ctacctaaaa acacaacata tcgtgcaccc cttttatatg catcaacaat
    17401 agcttcccta tcggaaaatc cattatatag aaattttgaa tatccaattc ttccaagggt
    17461 atataaatgt acattaggag aataatcacg taggcataaa atggtatcag atggttgaat
    17521 aatttttttt atctcatcag atttgctttg aagggttttt aaattaatcc catagtcata
    17581 attaaaccag cgccatgtat caatatctct ttttttaaga aaaatattat ttacaatcaa
    17641 ttttggattt gctgaaaagc gaacccaact gcatgtgtat gcatggagaa ctacaaaaat
    17701 tataaaaaaa tataatgcta gattaatctt ttttaaatct aggaaactaa aaaacgaatt
    17761 aattttataa agaaagataa aaaacaaaac attaaataca aaaagtatct ctattaaata
    17821 gtagtcatga acatcaaaaa cccaatacca aagaatgagg tatgaaaata aggcacctat
    17881 agacataact attaaccatt taagaaaagc gttaattgac ctagaaaata gtgcaacaac
    17941 accaaaaatt ataagcgcaa aaacaataac tctgtgatac gcataattta tcattgtatt
    18001 tattatatgc caaaaaattt catttcgctt ttcagcaggt atttcccata ttggacgaat
    18061 tgttgttgaa aataagtgag aatcatactt agtattataa taaattgcat aataatacca
    18121 tgcagctact aatgctaaag taagaatgaa tgagcctgaa agtaatgata tttttatttt
    18181 atattttctc caaaagtagt tattatcaaa aagttcataa aaaataaatg ctccaataaa
    18241 ggacataaaa accaatatgc ttgttatctt gataagtatt gctaaagtac taaaaacaaa
    18301 gaagcctatt aatccatata atttattatt ttgtaaaaat tgaaatacaa aaaagccagc
    18361 aatcaaattt aaagcaaatg aaattggatt agggataaaa ttgtgggcat atccaataag
    18421 aattggcgat gaaaacataa aaacgctata tataattgta tatttttgtg attttagata
    18481 atactgtact actgtcgcaa ataaaataat tcctaacaaa agaagaacaa aacttaataa
    18541 acgatacaaa aactcatttt gccctattac agaccatagt ttccccatca aaaaatatat
    18601 aattggaaat tcagacaccg cattcctgtt accgttttct aaaataagat ttgttttcgg
    18661 ttcaaaaaaa ggctcaccct tcatgtaatt ataggtaatt gacaaacaat ctgctttgcg
    18721 ccactcatgt ataggcttag gcccttcaaa aaaatcaata tagaaaccat ataaaaaagt
    18781 taaaactatc aatactaaca ataattttaa cctcaaatta atatccttta ggaatctaat
    18841 catatattac tattaaagcg ttctcgtttt atccaaaatt attaaacttt atcaaaaaga
    18901 aagacctttt gggttatttt ttatatttcg ctaatttatt tcattctaac tggaaattaa
    18961 acctatgata tgcttaaccc aaaaaacgct ttgtttataa cttaaatctt ccatagtaat
    19021 ccttacgctt gcaaaaaatg tttatatttg gtgacaataa gccaaactcc tcaaaatgat
    19081 tgacgtaaag ttaagcatag taaactcaaa gcccgagata ttcagaatta ttagggttga
    19141 tgaaaatatc aacctacaac tatttcatga ggtaatacaa gcagcctttg agtggagtaa
    19201 tatgcactca catttttaca atgccgataa gtcaatactc aagaatgagg aagaaatttt
    19261 attgggtaat gcattgaaca aatgggagag ccttatctat gtttatgatc tggccgattt
    19321 ttggacaata tcaatttcgc ttcttgctac aaattcaggt gagctaagca aaaaacccgt
    19381 ttgccttcaa gggcagggca aatcgccacc cgaggattct gggggtataa ttaattactg
    19441 tgaagtactt gaactccttg caaaacataa taagggcgat agacaatacc ctctaattgc
    19501 cgactggctt ggagaaaatt acaaccctaa cgagttcaat tgcgaacagg taaaccttaa
    19561 ctttaaaaat ctagtacttt agccatatgg agtaccgtgg cgaccccttg gtttaccaaa
    19621 cgctaaacga attaagtatt ccatttgaat actacgagca tcctgaggct cccactgttg
    19681 aaattgcctc taaatactgg gaagggataa attcatcgca ctgcaaaaat ctctttttca
    19741 ggaaccacaa aggaaacagg cactacctgg taattctgga ataccgtaag atgctgaaca
    19801 ttcatcaact cgaaaaacta ctaaaacaag gcaagctaac atttgcctcg tccgagcgta
    19861 tgaaacgcta cctggggtta acgccaggtt cggtatcgcc ttttggatta cttcacgaca
    19921 cctctcgcca tgtgcatctg tttatcgaca gtgaactaaa aaacactcaa cgacttagct
    19981 ttcacccaaa cctaaatact gcatcgctgg taattccaac catcgatttt ttcaggtatc
    20041 tcaactgggt tggaaatacg tatgagttta ttgattacgt aaatgagctg tagctatatt
    20101 tgcccaaata taccaggata gacaattctt tggcatgctg tgcgctgccc aagcatctct
    20161 ttgtacctat atcacttttt aatgtgattc ctcgactagc tcggaatgac aagcagagtt
    20221 gacagttgat ggttgatagt tgatagtgaa aaaaaaaaca agcactactc tcaattttcc
    20281 ctttaaactg cacactgtac actgcactct tttcaaaaga tccttcgctg cactcaggat
    20341 gacaaatgaa actattcgta taagtt
//
LOCUS       DAOSAL010000046        20121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_1897_length_20121_cov_49.573358, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000046 DAOSAL010000000
VERSION     DAOSAL010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 20121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20121
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1897_length_20121_cov_49.573358"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="PK049_07475"
     CDS             complement(<1..350)
                     /locus_tag="PK049_07475"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOK61436.1"
                     /translation="MENSRRFKILIADDDNISVILLTNYLKNIEADFIIARDGEEALT
                     LFEQNTDVDLILMDIKMPKLNGLEVTKQIKSKNPHTKIIAETAFAMVDDEKKAIEVGC
                     DAYITKPINGERLVA"
     gene            complement(478..1056)
                     /locus_tag="PK049_07480"
     CDS             complement(478..1056)
                     /locus_tag="PK049_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOK61437.1"
                     /translation="MEKSIKGTKTEQNLLKAFAGESQAKNRYTFFSKVAKEEGYEQIA
                     ELFMITAMQEEQHAKQFFKFLEGGMVEITASYPAGVIGTTAENLRAAAMGENEEWTSL
                     YPEFAKIAEEEGFPKIATAFKLIAKVEKEHEERYRKLLERVESGTVFEREEEIEWVCR
                     KCGYVHKGKKALKNCPVCNHPQAYFEEKADNY"
     gene            1172..1519
                     /locus_tag="PK049_07485"
     CDS             1172..1519
                     /locus_tag="PK049_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61438.1"
                     /translation="MVKSTRRLLISILLVIIALGNILIYSSYSHVHRINGQWVVHSHP
                     YDTQSDKQPVKNHKHTLSEIVLLAQAKQFEESSQNQVYNPEIAVTRIHLKTAVHHLQV
                     ALQLKPNRAPPFV"
     gene            1647..4043
                     /locus_tag="PK049_07490"
     CDS             1647..4043
                     /locus_tag="PK049_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOK61439.1"
                     /translation="MKKYLPILLVFIYINHIALRANELSEVCKLNGTVMSNGKALPFA
                     NVIVKGTTIGTVTDEAGSFALCDLKKGTYTITVSAVGFKTAEIKVDIDETDRKTVEIE
                     LQEDLLHLDEVVVSADRGMLKRTQAPVMVNTIAPALFASTQSVVVGEALNFMPGLRLE
                     NDCQNCGFNQVRMNGMEGPYSQILINSRPIFSGLAGVYGLELLPANMIDRVEVVRGGG
                     SVLYGSNAIAGTINIILKDASINSYEVGASGSLIGTGMARSNGPISDYTVNFNNTFVA
                     TNNRTGITIYGFTRDRNMFDANGDGYSELAPMSNVSLGTRFWQRVGERGKISADIFTI
                     REKRDGGNKQDYPLHERDVAEAVKHDMRAGSLIFERYFRQFDLLSIFASAQYLNRDSY
                     YGANQSLSSYGNSFDFTYNTGAQYKAIIGLSTLTAGVELTGSNLNDTKLAYRDLNSPI
                     FDIDPITGDTLGVSFPQVKNTTVSDQSLLTTGVFAQHDMGIGKWKTSVGLRAEQYRIE
                     DFAHPENGIKKGLVLVPRLSIMYNALPWVQTRVSYSQGYRAPQIFDEDLHVETSGARQ
                     VINRNDPNLKQETSHSFMLSADANREMGNFFIGFLAEIFYTRLQNPFYNEIGAPDENG
                     TVIYTRRNATDGAAVKGINLETKIKSFKDFSLTAGFTLQESLYDTPQEFNTREFFRTP
                     KQYGYLMLDWDFVKDLCLSVSGTYTSPMLAPYFGPNTNPDVGELRSTPDFYDVGVKLT
                     YNFKLEGKTLQLFGGVKNIFNSYQKDFDVGIDRDPSYIYGPTSPRTVYFGIKFGNVLK
                     "
     gene            complement(4117..7557)
                     /locus_tag="PK049_07495"
     CDS             complement(4117..7557)
                     /locus_tag="PK049_07495"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOK61440.1"
                     /translation="MNNSKPNPEEFYRLLAQSKERLKELNAINSVINITKENRPIPET
                     LHRVCFVLPDAWQYPEHTVARIKYGQYEFQTFGFKETPWCQRQGFESIDGVFGAIEIF
                     YTKQFPDEFEGPFLKEERDLLNNIANIIEGYINSIKGRDVLKEKTQAKRKPDLAEPQS
                     KRLLQRFINQHNADRDIYHDLMPFKVHEILLISTLYDAYSIEREDRLTDNILGEYSKL
                     SLSSVPRITGVSTLDEALEKLEERYFNLIIIMMGADTVTPLRMASRIKGEYKHIPLYL
                     LVNNSIIVNEIEKNPNTIEGVDRIFVWNGEPKIFFSMIKLLEDRANIENDTRIALTRV
                     ILLVEDSPKYYSRYLPLLYGSVLDQTKRVIEDVSTDDLYKVLRIRIRPKILLAGTYEE
                     AIDLFTRYKDYMLCMISDVKFFRDGRLDENAGVRLVEHARKMLPDLPIILQSFEKSNE
                     AIAHKLKVAFLDKNSESLLNDIKSFLSNYLGFGDFVFKDEAGNPIAIATTMEEFERAL
                     RIIPDESLLYHAQKNHFSMWLSARGEIQVARIIHPSKIDDFSGPAEIREYLLSTLKKY
                     RQEKRRGKIVGFDTNWEVDSSNIVSLADGSVGGKGRGLAFINTLLYTFDISQYTPNIN
                     LLTPRTSIIGTNEFECFMARNGLYDKVFACKSFEELQRHFIKAELSDQLKLRLDRLLQ
                     IYYRPLAVRSSGMLEDSIMQPFAGVFETYLIPNAHPDRSVRLQRLMNAIKLVYASVFS
                     PTARAYIKAMNLRVEDEKMAVIIQEVVGNKFENYYYPHISGVAQSHNYYPFGHINPDD
                     GFANIAIGLGKHVVEGGRSYRFCPKYPTLVNYTMEDLIKNSQVDFLAVDLSKCDYDLL
                     TGDEAGLARLDLYEAERHGTLKHCASVYSSENSSLIPGIGQSGPRVVNFANILKYNYV
                     PLAQTIDVILDIVEEALGAPCEIEFAVDLNRDANFRSSFFLLQIKPLMASAQEYRLDI
                     NTIDRAEAILLSNNAMGNGHLTNIEDVVYIRREAFNKAMTLEMANEVEWLNNTLEDEG
                     RKYILIGPGRWGTRDRWIGIPVAWSQICNAKVIVETSFEDFPLDASYGSHFFHNVISM
                     NVGYCSVPNGDSFSRIDWELLDSLPAVNETTFFRHVRFPKPLGVKMDGTQRLIVVTKG
                     "
     gene            7977..9332
                     /gene="gdhA"
                     /locus_tag="PK049_07500"
     CDS             7977..9332
                     /gene="gdhA"
                     /locus_tag="PK049_07500"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOK61441.1"
                     /translation="MKNVALQEKVNNFMAGLIARNPNEPEFHQAAREVIESIMPYIDE
                     NPIYQDEKILERITEPERIVIFRVPWIDDKGNIQVNRGYRIEMNSAIGPYKGGLRFHP
                     TVNLSILKFLAFEQVFKNSLTSLPMGGGKGGSDFDPKGKSDKEVMRFCQSFMTELYRH
                     IGPDTDVPAGDIGVGGREIGYLFGQYKRIVNEFTGVLTGKGLKWGGSLIRPEATGYGN
                     VYFAQEMLKTRGESFKDKIVAVSGSGNVAQYTVEKVNELGGKVVTLSDSDGFIYDPKG
                     IDNEKLKFVMWLKNEKRGRIKEYAEKYGCDYFPAERPWGIKCDIALPSATQNEINGEE
                     ARTLVRNGCFCVSEGANMPSTPEAVDVFLNAKILYGPGKAANAGGVATSGLEMSQNSL
                     RLSWTREEVDQRLHQIMINIHETCRKYGTKEDGFINYVDGANIGGFVKVADAMIAQGV
                     V"
     gene            9549..10082
                     /gene="nuoE"
                     /locus_tag="PK049_07505"
     CDS             9549..10082
                     /gene="nuoE"
                     /locus_tag="PK049_07505"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOK61442.1"
                     /translation="MRVQRILTKPASSIPADEVDLALIEPLIKKYKGKKGNLIPLLQG
                     VQSIYGYIPRSAFELIAHETGLTLSDMYGVATFYAQFRLSPVGKHIIKVCHGTACHVQ
                     GANAISTALKEALKVNDGETTPDKLFTLETVACLGCCSLAPVMMIDEETYGKLTGSSA
                     VKIVKDIKIKETQAKGE"
     gene            10130..11908
                     /locus_tag="PK049_07510"
     CDS             10130..11908
                     /locus_tag="PK049_07510"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOK61443.1"
                     /translation="MAETKVIVGLGSCGIAAGANKVYNRLKALKEADNLDFELAKTSC
                     IGMCFREPLVEVVDENGSMIYGDVDEDKAIEIIQQHVLGHNPIKEYVVKTDLFPTPDQ
                     SYFDGQVKIALRNCGYIDPENIHEYEARGGYQALRKIANEALLPEQIIQTIIDSGLRG
                     RGGGGFPTGMKWRFARANQSEEKYIICNADEGDPGAFMDRSLLEGDPHAVLEGMIIGA
                     YAIGATEGVIYCRAEYPLAIKRLNIALAQAREHGYLGKNIMGIQGFNFDIFIKEGAGA
                     FVCGEETALMASIEGKRGMPSKRPPFPAEKGLWQKPSNINNVETYANVPYIVANGAES
                     YNKYGTEKSKGTKVFALTGKIKHGGLVEVPMGTPLRDIIYKLGGGIQNDKKFKAVQLG
                     GPSGGCIPEHLIDIQVDYEQVTATGAIMGSGGMVVMDETTCMVDVARFFLDFTQKESC
                     GKCTFCRIGTKRMLEILTRITEGEGQEGDIEKLEELALQVRESSLCGLGQTAPNPVLT
                     TIKYFRHEYEAHIRDKKCPAHSCKKLLTYEIDPNKCTGCTVCAVKCPVKAIDGERKKV
                     HHINQDICIKCGDCYNSCRFDAITKS"
     gene            11947..15660
                     /locus_tag="PK049_07515"
     CDS             11947..15660
                     /locus_tag="PK049_07515"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOK61444.1"
                     /translation="MSELVNIVLNGKNTTASKGEYILDVARRHGIEIPTLCNDPRLEP
                     YSSCYVCVVEIEGQRNLQPSCSTRVAEGMKIITDNERVYKARKTALDLIMSNHYADCV
                     APCRERCPAGVDVQGYISLIEKGLYSEAIGLIKQVNPLPAICGRVCVRPCEAACRRNY
                     MDEGNGVGIDYLKRFAADRDMESEHHYKPTIAPSTGKKVAIIGAGPGGLSAAYFLQQK
                     GHQCDIYEAQPHAGGWLRYGIPEYRLPNDILDKEVSTITELGVKIFYNQRLGKNLSYK
                     TIAENYDATILTIGSQRGQLLGAEGDDADGVFSGIDFLRNMEVTGQRYDFKGKRIAVV
                     GGGNTAMDCCRTSIRCGSTDVTVIYRRTEAEMPANPIEIHESKLEGVKYMFLTTPVRV
                     NKTADGKVKSMTLIRMELGEPDASGRRRPVPVEGSEFEMEFDYILAAIGQKTEVDFLD
                     DINKHSRHGELKVTKWGDIEADRQTLQTGIPSVFAAGDGVTGPATIIEAIAQAKLASH
                     SCHLYLTGQPVVPPRKEFLSKKDNFRKLSSNDFVTRYQKQQREEMPVMDANQRVNFKE
                     VELGYTHEQAMHETARCLECGCVEYFECDLKRHADTYQADQLRFMGDHKEFDVNFTHP
                     FIEIDNNKCILCSRCVRVCKEIVGANALTLVKRGFDTFVAPAFGDNLADTTCESCGLC
                     ISACPTGAITENVPFKPGPVKTDKFETICGYCSVGCTLNFHHKSGYLMRVTGANGQVN
                     TDANLCMYGKFGYREFNSINRLTKPLKKEGDKFVEISYQEAFDIITQKIKSVAPDANA
                     FFAGAILTNEEQYLVQKLARTGAKTNNVGSFHYLGTGNGFTSDSIQSSMFGDISKANH
                     IYLFATQINRYNAVAGYMVNASKKPVTVIAKEESNLSNRSKEFIKVDSYYHFIKAVNH
                     YLLSGNKQNMVFIGDHCEGFGAYSANLLLEDYANLCEQAGCERSVIERFADEFNNDYG
                     ALLIFAEAEVSDNTAAEIMNLMLITGKLGKTAGGLIALKPKNNSHGLSDMGLNPNWGL
                     GLQDITNQEFADKFKRKWGVESLPSKKYNLVELLEKGSLTNILIVGEDPIGTAKHKAA
                     VKDYLQKVRFKVVIDAFLTETAQIADIILPASLWFESGGSFTNTNHTIQQFEVGVKPK
                     VELTTADLLTKLLNRFGVNSPTDIYDIQTEILSLLPQTESGKHLQFIQTTSEGQHLQF
                     HAGCSYLMHNFDVEFENKLINAKTHSHERVFQH"
     gene            15638..16093
                     /locus_tag="PK049_07520"
     CDS             15638..16093
                     /locus_tag="PK049_07520"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOK61445.1"
                     /translation="MKEFSNINDILDFAIAREQDAVDFYNRLAAKARNNDMRHIFEQF
                     AREEMMHKARLAKIKEEGVITLPTEKVVDLKIADYVVLDSERDDMTYEQALVLAMKRE
                     KAAFKLYLALSEKVDKTEYKELFKQLAQEESRHKLRFELEYDEYVLKDN"
     gene            16486..16812
                     /locus_tag="PK049_07525"
     CDS             16486..16812
                     /locus_tag="PK049_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HOK61446.1"
                     /translation="MSFARVYPLYLTKIERKGRTREELNVIIKWLTGFDTATLESLIE
                     QNVTFEEFFRLATINPNAGLITGKVCGIKVEEIKNPLTQKIRYLDKLVDDLAKGKSIE
                     KIIPKP"
     gene            16838..19189
                     /locus_tag="PK049_07530"
     CDS             16838..19189
                     /locus_tag="PK049_07530"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOK61447.1"
                     /translation="MKRNYYRSNFILSVSAAVLTAIIFIFLYYTIGVRQRRFIYNDSK
                     WIAIQQSKNAASQVESYFINAIHSARSLVKQTKVLYEKRADRSEIVSLIHSYTKDNPD
                     FLAVWTMWEPNAFDGKDKQYRNTPPYDKEGHLSVTFFRMADSIVFEYTEPEDYNEFYY
                     TIPKERNSEMVLEPYLYQYQGYPIEFYETSIVVPIIIKNKFCGVYGIDLDLNYLQRKL
                     SEIKLQTKGHVSLISSDGEIVTHPDTAFIGKNIFSLAGTNDTLARYIVKSGLEQNYET
                     VCNFTKSEVFRTFYPIRVGKSDTQWSIMVQLDKREATLRSIYLHNIALGLLIAGLILI
                     SYLIYNIFDRLRYEHQLILAKESAEQKERELQKMYYELQTSEEELRATNEELFATSET
                     LRESNLMLTGAKEKAEEASRLKTAFLHNLSHEVRTPLNAIYGFSQMLNNIDISADESK
                     KFIQIIQDSSKQLVSIISDIITISSIETGQERITNTNIHLNTLLDELYQTFSAKLANK
                     PVELRVYKEFSDNEDEIFVDDLKLRQVLHHLLLNAVKFTHNGSIEFGYRLKSSNLEFY
                     VKDTGIGIEGKKQDVIFERFTQADENIHAEYGGTGLGLSICKGFLNLMGGAIWVESEL
                     GQGATFYFTLPYHPITSKESKGISIQSQTNISEMKTILVAEDDINSFKLLKAVLKDFD
                     FHLLHADNGRKAVELCQNEDSICLVLMDIKMPELNGVLATKLIKELKPTLPVIAQTAY
                     ATLDDIEKNREVFDAYITKPLDIEQLKKIILEFCDRFGKSKKY"
     gene            complement(19868..20050)
                     /locus_tag="PK049_07535"
     CDS             complement(19868..20050)
                     /locus_tag="PK049_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61448.1"
                     /translation="MGTPRVELPRDTIRHACSVAHMVSLTRPTATFHPRPIAGSGFYA
                     SACFFRSIPPLRKMVG"
BASE COUNT         5871 a         4513 c         4467 g         5270 t
ORIGIN      
        1 gccaccaacc tttcgccatt tattggctta gtgatgtagg catcacagcc tacctcaatg
       61 gcctttttct catcgtcaac catggcaaag gctgtttcgg caataatttt tgtgtgagga
      121 tttttggatt ttatctgctt tgtgacctct aacccgttca gcttgggcat ttttatatcc
      181 ataaggataa ggtcaacatc ggtgttttgc tcaaaaaggg taagtgcttc ctcaccatcg
      241 cgggcaataa tgaaatccgc ttcaatgttt tttaggtaat tggtaagcag tattaccgaa
      301 atattatcgt cgtcagctat cagtatctta aaccttctgg agttttccat ccccctaatt
      361 ttactcagac gttcattcta aagcgcaaag ttaaaacgct ttaaattaat acacaactaa
      421 ttgttcttaa acataaaaaa accgcagcaa ggctgcggtt aaataattaa tggcatacta
      481 gtaattatca gctttttcct caaagtatgc ctgaggatgg ttacaaacag ggcaattctt
      541 tagagccttt ttccctttat gaacgtaacc gcatttacga caaacccatt caatttcctc
      601 ctcgcgctca aaaacagttc cgctctcaac ccgttcaagc agtttgcggt aacgctcctc
      661 atgctctttt tcaacctttg caatcaattt aaaagccgtt gcgatctttg gaaaaccctc
      721 ttcctcggct atcttagcaa attcagggta taggcttgtc cactcttcgt tttcacccat
      781 ggcggcagcc cttaagttct cggcagtagt gccaataact cctgcaggat aacttgcagt
      841 tatttcaacc atgccaccct caaggaattt gaaaaactgt ttggcatgtt gctcttcctg
      901 catggcggtt atcatgaaca actcggctat ttgttcatag ccttcctcct tggcaacctt
      961 tgagaaaaag gtgtaccggt tctttgcttg cgattcaccg gcaaaagcct ttagtaggtt
     1021 ctgttcggtt tttgttccct ttatgctctt ttccattgct attactggtt tatgtttata
     1081 gttttgctaa aatacgcaaa aaacaccaat aaaccattgt ttagaataat tctaaagtta
     1141 cctttgcagt gttaattcgc taacagccga gatggtgaaa agtactagaa gactgcttat
     1201 atcgatactc ctggtaataa ttgcgctggg gaatattctt atttacagca gctacagcca
     1261 cgttcaccga attaacggac aatgggtagt ccactcacac ccatacgata cacaatccga
     1321 taagcagccc gttaaaaacc ataagcatac cttatcggaa attgtattgc ttgcccaggc
     1381 aaaacaattc gaggaaagct cacaaaacca ggtttataat cccgaaattg ctgttacccg
     1441 aatacatcta aaaacagcag tacaccacct tcaagtagca ttacagctta aacctaatag
     1501 ggcaccccca tttgtataaa taaccacaat gtaaaaccag cagtaaacct gaaaccattc
     1561 gggttcctat actgatgctt gcaatgcatc ggcttgcatg gttatagaaa tcaaatcagc
     1621 attaacttcg ttaatttttt ttcagcatga aaaagtattt accaatacta ttggtattca
     1681 tatacatcaa tcatatcgca ctaagggcaa atgaactatc ggaagtttgc aagttaaacg
     1741 gaacagttat gagcaatggt aaagccctac cctttgcaaa tgtcattgtt aaaggcacta
     1801 caatagggac tgtaaccgac gaagcgggca gctttgcact ttgcgacctg aaaaagggaa
     1861 cttataccat tacggttagc gcagtgggat ttaaaactgc cgaaatcaag gttgatatag
     1921 atgaaaccga caggaaaacc gtggaaattg aattgcagga ggacctctta caccttgatg
     1981 aggttgtggt ttcggccgat aggggtatgc ttaagcgtac ccaagcccca gtaatggtaa
     2041 acaccattgc acctgcactt ttcgcttcaa cacaatcggt tgtggttggt gaagccctaa
     2101 actttatgcc aggcctaagg cttgagaacg attgccagaa ctgcggattc aaccaggttc
     2161 gcatgaatgg catggagggc ccctacagcc aaatactcat caatagtcgg ccaattttta
     2221 gcgggctggc aggcgtttac ggtctggaac tgctccctgc aaacatgatt gaccgtgttg
     2281 aggttgtgcg tggtggtgga tcggtgcttt atggtagcaa tgctatagcc ggaacaataa
     2341 acatcatttt aaaagatgcc agcataaact cctatgaggt tggggcatcg ggttcgctga
     2401 ttggaactgg gatggcacgt tcaaatgggc ctatcagcga ctataccgtt aactttaaca
     2461 acacatttgt tgctacaaac aaccgtacag gcataaccat ttacgggttt actcgcgaca
     2521 ggaacatgtt cgatgccaat ggcgatggtt actcggaact tgcacccatg agcaacgtaa
     2581 gccttggcac ccgattctgg caacgcgttg gcgagagagg taaaattagc gccgacattt
     2641 tcactattcg ggagaaacgc gatggtggaa acaaacagga ttacccgttg cacgaacgcg
     2701 atgtagccga agctgttaaa cacgacatgc gtgcaggttc gctaatcttt gagcgttact
     2761 tcaggcaatt cgacttactc tcgatctttg cctcggcaca gtacctgaac cgcgactcat
     2821 actatggggc aaaccagtcg ctgagcagtt acggcaactc attcgatttt acctataaca
     2881 ccggggcaca gtacaaagca attattgggc tatcgaccct gaccgctgga gttgaactta
     2941 ccggcagcaa ccttaacgac accaagctgg cttaccgcga tttaaacagt cccatttttg
     3001 atattgatcc aataacgggc gatacgttag gagtaagttt cccacaggtt aaaaacacaa
     3061 ccgtttcgga ccaatcgcta ttaacaactg gtgtttttgc ccagcacgac atgggtattg
     3121 ggaaatggaa aacttcggtt ggcttgaggg ctgaacagta ccgtattgaa gattttgccc
     3181 atccggaaaa tggaataaaa aaaggattgg tgcttgtgcc acggctgagc attatgtaca
     3241 atgcgcttcc gtgggtacag accagagtat cgtactcaca gggctaccgt gctccacaga
     3301 tatttgatga ggacttgcat gtggaaacct ccggtgcccg tcaggtaatc aacaggaacg
     3361 atcctaacct aaagcaggaa accagccaca gctttatgct ctcagctgac gcaaaccggg
     3421 aaatggggaa cttttttatc ggctttttgg ctgagatctt ttatacacgc ctacaaaatc
     3481 cattctataa cgagataggt gcacccgacg aaaacggaac ggtaatttac acccgtcgca
     3541 atgccaccga tggtgcagcg gttaagggga taaaccttga aaccaaaatc aagtcgttta
     3601 aggacttttc acttaccgcg ggttttaccc tacaggaatc gctttacgat accccccagg
     3661 agtttaacac gcgtgagttc tttcgtactc ccaagcaata tggctaccta atgctcgact
     3721 gggactttgt aaaggattta tgtctatcgg tttcgggcac atatacaagc ccaatgcttg
     3781 ccccctactt tgggccaaac actaatcccg atgtagggga actccgctca actcctgact
     3841 tttatgatgt aggggttaag ctaacctata acttcaagct tgagggtaaa acgcttcagc
     3901 tattcggtgg ggtaaagaat attttcaact cctaccaaaa ggattttgat gtgggtattg
     3961 atagggatcc atcgtacatt tacggcccca catcgccacg aacagtttac tttggaataa
     4021 agtttggaaa tgtactgaag taaacacaaa caaaatcccc ctcagagggg gattttgttt
     4081 tttgccaaac tgcttcaccg agcaaaacgg ctattactat cctttagtca ccacaatcaa
     4141 ccgttgtgtg ccatccattt taacacccaa aggttttggg aagcgtacat gccggaaaaa
     4201 tgttgtttca ttaactgcag gtaggctatc gagcagttcc caatcgattc gtgagaatga
     4261 gtcgccattg ggaacagagc agtaacccac attcattgag ataacgttgt gaaagaagtg
     4321 tgagccgtaa gaggcatcga gagggaaatc ctcaaagctg gtctccacaa taaccttggc
     4381 attgcaaatt tgagaccagg caaccggaat acctatccac ctgtcgcgag ttccccatcg
     4441 acctggccct ataaggatat actttcgccc ttcgtcctca agtgtattgt tgagccattc
     4501 cacctcgtta gccatttcca aagtcattgc cttattgaat gcctcgcggc gaatatatac
     4561 tacatcctca atgttggtta ggtgtccatt ccccattgca ttattggaga gcaaaatggc
     4621 ttcagcccga tcaatagtgt taatatcgag cctatattcc tgagcgcttg ccattagcgg
     4681 cttaatctga agcaggaaga acgatgacct aaagttagcg tcgcggttaa ggtcaacagc
     4741 aaactcaatt tcgcaaggag cgccgagagc ctcctcaaca atatcgagaa tcacatcgat
     4801 ggtttgggca aggggtacgt agttatactt taggatattg gcaaagttta ccactcttgg
     4861 ccccgattgc cctatacccg gaatgagcga ggagttctcg gagctgtaaa ccgatgcaca
     4921 atgcttgagg gttccatgcc tttcggcctc gtagaggtcg aggcgggcaa gtcctgcttc
     4981 atcgccggtt agcaagtcgt aatcgcactt gctgaggtca actgccagaa aatcgacctg
     5041 tgaattttta ataaggtcct ccatggtata gttgaccaac gttgggtatt ttgggcaaaa
     5101 ccggtatgag cgacctccct ccaccacgtg ttttcccaaa cctatggcaa tgttggcaaa
     5161 tccatcgtcg gggttgatat gcccaaacgg gtagtagtta tgcgattggg ctaccccact
     5221 gatgtgtgga tagtagtagt tctcaaactt attgcccaca acctcctgaa tgataacggc
     5281 cattttttca tcctcaaccc taaggttcat ggctttaatg taagcccttg ccgttggtga
     5341 aaataccgat gcgtaaacca gcttaatggc gttcatcagg cgttgtagcc tgacgctcct
     5401 gtcgggatga gcatttggga taaggtaggt ttcaaatacc ccggcaaagg gctgcatgat
     5461 tgaatcctca agcatacccg atgaacgcac agccagcggc ctgtagtaaa tctgcaagag
     5521 cctgtcgagc ctaagcttta gctgatccga aagctcggcc tttataaaat gcctttgcag
     5581 ctcctcaaag cttttgcaag caaacacctt atcgtatagc ccattgcgtg ccatgaagca
     5641 ctcaaactcg ttggtgccaa ttattgaagt tcggggtgta agcaggttga tattgggcgt
     5701 atactgcgaa atatcgaagg tgtagagcag ggtattgata aaagcaagcc ctcgaccctt
     5761 gcccccaacc gaaccatcgg caaggctaac aatattgctg gaatcaacct cccagttggt
     5821 atcaaaacct acaatcttgc cccttcgctt ctcctggcgg tattttttca gggtgctaag
     5881 caggtactcc cggatctcgg ccgggccgga aaaatcgtca atctttgacg ggtgaatgat
     5941 acgggccacc tgtatctcac ctcgtgccga gagccacatg gaaaagtggt ttttttgggc
     6001 atggtaaagt aagctctcat cgggtatgat acgcagcgcg cgctcaaact cctccatggt
     6061 tgttgcaatg gcaatgggat tcccagcctc gtccttaaaa acaaaatcgc caaaaccaag
     6121 atagttgctc aaaaaacttt taatatcgtt gagcaaactc tccgagtttt tgtccaaaaa
     6181 agcaaccttt agcttatggg caatggcttc gttcgatttt tcgaacgatt gaaggattat
     6241 tggtaagtcg gggagcatct tacgggcatg ctcaaccaac cggacaccag cattttcgtc
     6301 gagtcttcca tcgcggaaaa actttacatc ggaaatcatg caaagcatgt aatccttgta
     6361 acgggtgaaa aggtcaatgg cctcctcgta tgtgccagcc aaaagaattt ttggacgaat
     6421 tcggatacgc agcaccttgt aaaggtcatc ggtgcttacg tcctcaataa cccgcttggt
     6481 ttggtctaac accgagccat acagtagtgg taagtatcgc gagtagtatt ttggggaatc
     6541 ctcaaccagc aaaataacgc gcgttaaggc tattcgggta tcgttttcaa tattggcgcg
     6601 atcctcaagc agctttatca ttgagaagaa tatcttgggc tcgccattcc atacaaaaat
     6661 cctatcgacc ccctcaatgg tgttggggtt tttctcaatc tcattaacaa taatgctgtt
     6721 gttaacaagc aggtaaaggg gaatgtgctt gtactcgccc ttaatacgtg aagccatgcg
     6781 taagggggta accgtatcgg cccccatcat gataatgata aggttaaagt atcgctcctc
     6841 gagcttctcc agggcttcgt cgagtgttga tacccctgta atacggggca cgcttgagag
     6901 gctcaacttg gaatattcgc caagtatgtt atcggttagc ctatcctcgc gctcaatgct
     6961 gtatgcatcg taaagggtgg agataagcaa tatctcatga accttaaagg gcatcaggtc
     7021 gtggtaaata tccctatcgg cattatgctg gtttataaac cgttgcagta gtcttttgct
     7081 ttggggttca gcgagatcgg gcttgcgttt agcctgtgtt ttttccttta aaacatcgcg
     7141 cccctttata ctgttgatgt agccttcaat aatgttggca atattattaa gcaggtcgcg
     7201 ctcttccttc aggaaaggcc cttcaaactc atcggggaac tgtttagtat aaaaaatctc
     7261 aattgcccca aagacaccgt cgatgctttc aaaaccctga cgttgacacc atggggtttc
     7321 cttaaatcca aaggtttgga actcatactg cccgtatttt attcgggcaa cagtgtgttc
     7381 cggatactgc caggcatcgg gtaaaacaaa gcaaaccctg tggagggtct caggaattgg
     7441 tctgttttcc ttggtaatat tgataacgct attaatggca ttcaactcct ttaaccgttc
     7501 cttgctttga gcaagtaggc ggtaaaactc ctcgggattt ggcttgctgt tattcatcgt
     7561 atccaacttt tatctttatc ctttaacctt tatcctttaa cctttatcct ttaaccttta
     7621 tcctttaacc tttatccttt gccctttatc ctttaacctt gaactattaa cctcgaacct
     7681 ttatccttcg taaatacagg tggtattgca tatcgttcta tcccgtttaa cggttcacgg
     7741 ttcacgattc acgttttagc ctttatcctt caaattcaac ctgtaattta ggtataccac
     7801 gcaataaagt caaattattt aggcaaaata agaaatactc gtaatcggcc tacgtgtttc
     7861 acgtagaaga cacttccgta attcgctgtt tttaaatcac atctatttac atcgcttaaa
     7921 aaaggtctta attttccatt cagattttca tgtttaacca accaaactaa tacaatatga
     7981 aaaacgttgc tttacaggaa aaagtgaaca actttatggc aggcctaatt gcccgcaacc
     8041 ctaatgagcc cgagttccat caggctgccc gcgaggtaat tgagagcata atgccctaca
     8101 ttgatgagaa tccaatttac caggatgaaa aaatccttga gcgcatcacc gaaccagagc
     8161 gcatagtaat tttcagggtt ccctggattg acgacaaggg taacattcag gttaaccggg
     8221 gttaccgcat tgagatgaac agcgccattg gcccctacaa gggcggattg cgtttccacc
     8281 ccacggtaaa tttgagtatt ctgaagtttt tagcatttga gcaggttttc aagaacagcc
     8341 taacatcgtt accaatgggt ggcggcaagg gtggctccga tttcgacccc aaggggaaga
     8401 gcgacaagga agtaatgcgt ttctgccaaa gctttatgac cgagctatac cgccacattg
     8461 gccccgacac cgacgtacct gcaggtgata tcggcgtagg tggacgtgaa attggctacc
     8521 tgtttggcca gtacaagcgc attgtaaatg agtttaccgg tgtgctcaca ggcaagggtt
     8581 taaagtgggg cggttcgctc attcgccctg aggctaccgg ttacggcaat gtttactttg
     8641 cccaggaaat gcttaaaacc cgtggcgaaa gctttaagga taaaattgtt gccgtttcgg
     8701 gttcaggcaa tgttgcccag tataccgttg aaaaggttaa cgaacttgga ggcaaggttg
     8761 ttaccctttc cgattccgat ggatttatct acgatcctaa gggaattgac aacgaaaaac
     8821 taaaatttgt gatgtggctg aagaatgaga aacgcggacg tattaaggag tatgccgaaa
     8881 agtatggctg cgactatttc ccagcagaac gcccatgggg tattaaatgc gatattgcat
     8941 tgccatcggc cactcagaac gaaattaacg gcgaggaggc aagaacgctt gttcgtaacg
     9001 gttgcttctg tgtatcggaa ggagccaaca tgccttcaac ccccgaggct gtggatgttt
     9061 tcctaaacgc caaaattctt tacggcccgg gtaaagccgc caatgctggt ggtgtagcca
     9121 cctcggggct tgagatgtcg caaaactcgc ttcgcttgtc atggacccgc gaggaggttg
     9181 accaacgcct gcaccaaatc atgataaaca tccatgaaac atgccgtaaa tacggaacca
     9241 aagaggatgg atttatcaac tacgttgatg gcgctaacat cggtggtttt gttaaagttg
     9301 ccgatgccat gattgctcag ggtgtagttt aaatgatttg agatgaaaac tatttgcgct
     9361 aacttatagt tttgtaacaa acggagcatg aggcccattg ccttgtgctc cgtttttcgc
     9421 tttgaaaaac atcccctttt attgcccatg aatagaacaa tcaaaccaaa aaagtctttt
     9481 ttttgtattg agtaatcaat atgttttcat atcaacctaa aacattgaat atctaatcat
     9541 tcttaaccat gcgagttcaa cgtatactca caaaaccagc aagtagcatt cctgccgatg
     9601 aagtcgattt agcgctgatt gagccactta tcaaaaagta taaggggaaa aaaggaaatt
     9661 taatcccatt gctacaaggg gtacagtcca tttacggcta tattccccgc tctgcctttg
     9721 agctaattgc ccatgaaacc ggcttaaccc tgagcgatat gtatggagtg gctacattct
     9781 acgcacaatt tcgcctatcg ccggttggta agcatatcat taaggtttgt catggcactg
     9841 catgccacgt tcagggcgcc aatgccattt caacagccct gaaagaggcc cttaaggtaa
     9901 acgacggtga aaccacgccc gataagctat ttacgctcga aacagtagcc tgcttaggat
     9961 gctgttcgct tgcaccggta atgatgattg atgaagaaac ctacggaaag cttaccggta
    10021 gctcagctgt gaaaatagtt aaggacatta agataaagga aactcaagct aaaggtgagt
    10081 aacccacaag ttacatcaac ctttaaaaac aatataacgc gtaataaaaa tggctgaaac
    10141 aaaagttatt gttggcttag gtagttgcgg aattgcagcc ggagccaaca aggtatacaa
    10201 taggttaaaa gctctaaagg aagccgataa tttggacttt gagctggcaa aaaccagctg
    10261 tattggcatg tgttttcgcg aaccacttgt tgaggtggta gatgagaatg gatcaatgat
    10321 ttatggcgat gttgatgagg acaaggccat tgaaattata cagcaacacg ttttgggaca
    10381 caatcccatt aaggagtatg tggtaaaaac cgatttattc cccacccctg atcaatcgta
    10441 ttttgacggc caggtcaaga ttgcgctgag gaattgtggc tatattgacc ccgagaatat
    10501 ccacgaatac gaggcacgag gtggttatca ggctttaaga aagattgcta acgaagccct
    10561 tttgcctgag caaatcattc aaaccattat tgactcaggc ctacgtggcc gtggtggtgg
    10621 aggttttcca accggcatga aatggcgctt tgcccgcgcc aatcagtcgg aagagaagta
    10681 catcatttgc aacgccgatg agggcgaccc aggcgcattc atggatcgtt cactgctcga
    10741 aggcgacccc catgctgtgc ttgaagggat gataatcggc gcatacgcta taggcgccac
    10801 cgagggggta atatactgcc gagcagagta tccattggcc attaaaaggc taaatattgc
    10861 tttggctcag gctcgtgagc atggctacct gggcaaaaac atcatgggaa tccaaggttt
    10921 caatttcgat atcttcatta aggagggtgc cggagctttt gtttgcggcg aggaaacagc
    10981 ccttatggct tccattgaag ggaaaagggg tatgccatca aaacgtcctc ctttccctgc
    11041 tgagaaaggt ctttggcaaa aaccatcgaa tatcaacaac gtggagacct acgccaatgt
    11101 accttacatt gttgcaaacg gagccgagtc gtacaacaag tatggcaccg aaaagagcaa
    11161 gggaactaag gtatttgccc taacaggcaa aataaaacac ggtggacttg ttgaagtacc
    11221 catgggaacg ccgctgcgcg atattatcta caagcttggc ggtggcatac aaaacgataa
    11281 aaagttcaag gccgttcagc ttggaggccc ttcgggcggt tgtattcccg aacatcttat
    11341 cgacattcag gtcgattatg aacaggttac cgcaaccgga gccatcatgg gttctggggg
    11401 catggtagtg atggacgaaa ccacctgcat ggttgatgtg gcaaggttct tccttgattt
    11461 cacccaaaag gaatcgtgtg gcaagtgcac cttctgccgc attggtacca aacgaatgct
    11521 ggagattctc acccgcatca ccgaaggcga aggacaggaa ggcgatattg aaaaacttga
    11581 ggaattagcc ctgcaggtaa gggagagttc tctgtgcggc ttgggtcaaa cagcacctaa
    11641 ccctgtactt accacaatca agtatttccg gcacgagtat gaggctcaca tccgcgacaa
    11701 gaaatgccca gcccacagct gcaagaaact acttacctat gagattgacc ccaacaagtg
    11761 taccggatgt accgtttgtg ccgtaaaatg cccggttaaa gccattgatg gcgaacggaa
    11821 aaaagttcac cacatcaatc aggatatttg cattaaatgc ggcgattgct acaacagctg
    11881 ccgtttcgat gctattacca agagttaacc aaacgttgat gattaaacgc attaaattga
    11941 tttgaaatga gcgaattggt caatatagtt ctaaacggta aaaacacaac cgcaagcaaa
    12001 ggcgagtata ttcttgatgt tgcccgccgt catggcatcg aaatccctac cctttgcaac
    12061 gacccaaggc ttgagcccta ctcgtcgtgc tatgtttgcg tggttgaaat tgagggtcaa
    12121 cggaatctcc agccctcttg ctccaccagg gtggccgaag gcatgaaaat aatcactgac
    12181 aacgagaggg tttataaggc gcgtaagaca gcgctcgacc taataatgag caaccattac
    12241 gccgactgtg ttgcaccctg tcgcgaacgc tgcccggctg gtgttgatgt tcagggctat
    12301 atctcgctga ttgagaaagg actttacagt gaggccattg gtttaatcaa acaggttaac
    12361 cccctacctg caatctgtgg tagagtatgc gttcgccctt gtgaggctgc atgccgtagg
    12421 aattacatgg atgagggcaa cggggttggt atcgattacc taaaacgctt tgcggccgac
    12481 cgcgatatgg aatcggagca ccactacaaa ccaaccattg caccctctac agggaaaaag
    12541 gtggcaatta ttggggctgg tcccggtgga ctgtcggcag catacttcct tcagcagaaa
    12601 ggtcatcagt gcgacatata tgaagcccaa ccccacgcag gggggtggct ccggtatgga
    12661 ataccggaat atcgtttacc taacgatata ctggacaaag aggtgtcaac cattaccgaa
    12721 cttggtgtaa aaatctttta caaccagcgc cttggcaaga atttaagcta caaaacgata
    12781 gccgagaact atgatgcaac catactaacc attggctccc aaaggggaca gttgcttggc
    12841 gctgaaggcg atgatgccga tggagttttt tccggcatcg atttcctacg caacatggag
    12901 gttactggcc agcgttacga cttcaaaggt aaacgcattg ccgttgttgg cggtggtaac
    12961 acagcaatgg actgctgcag aacctcaatc cgatgcggta gcaccgatgt aaccgttatc
    13021 tatcgccgaa ctgaggccga aatgcctgca aacccaatag aaatacacga gtcaaaactc
    13081 gaaggtgtaa agtacatgtt cctaaccaca ccggttaggg taaacaaaac cgccgatggc
    13141 aaggtaaaat cgatgactct tatccgcatg gagcttggcg aacccgatgc atcagggcgt
    13201 cgacgccctg ttccggttga gggctcagag tttgaaatgg agttcgacta tatcctggca
    13261 gccattggcc aaaaaaccga ggttgatttc ctcgacgaca tcaacaaaca ctcccggcat
    13321 ggtgaactga aggttaccaa atggggcgat attgaggctg ataggcaaac cctccaaaca
    13381 ggcataccat cggtttttgc tgcaggcgat ggcgtaaccg gtcctgcaac cattatcgag
    13441 gcaatagcac aggctaaact tgcctcgcat agctgccact tgtaccttac gggacagcca
    13501 gttgttccac cccgcaagga gttccttagc aagaaggata atttccgcaa gctgtccagc
    13561 aacgattttg taacccgata ccaaaaacag cagcgcgagg aaatgcccgt aatggacgca
    13621 aatcagcgtg tcaacttcaa ggaagttgag cttggctata cccatgaaca agccatgcac
    13681 gaaactgctc gctgcttaga atgtggatgt gttgaatact ttgagtgcga cctaaaacgc
    13741 cacgccgata cctatcaggc cgaccaactc cggttcatgg gcgaccataa ggagtttgac
    13801 gttaacttca cccacccttt catcgaaatt gataataaca agtgtatact ttgctcaagg
    13861 tgcgtccgcg tttgcaagga aatagtggga gccaacgccc ttacgcttgt taagcgtgga
    13921 ttcgacacat ttgtagcacc cgcctttggc gacaacctgg ccgacaccac ttgcgaatcg
    13981 tgtggtttgt gtatttctgc ttgccctact ggagctatca ctgagaatgt accttttaag
    14041 cccggacccg ttaaaaccga taaattcgaa accatttgtg gatactgctc tgtgggctgt
    14101 acgcttaact tccaccataa gagcggatac ctaatgcgtg taacaggtgc taatgggcaa
    14161 gtgaacaccg atgcaaacct atgcatgtac ggtaagtttg ggtacaggga gttcaatagc
    14221 atcaaccgtt taaccaagcc actaaaaaaa gagggagaca aatttgttga aatcagctac
    14281 caagaggctt ttgatataat cacccagaag attaaatccg tagcccccga tgcgaacgcc
    14341 ttttttgccg gtgcaatttt aaccaacgag gagcaatacc tggttcagaa acttgctcgg
    14401 acaggcgcaa aaaccaataa tgttggtagt ttccattacc ttggcacagg caacggattt
    14461 acgtctgaca gtatccagag ttcaatgttt ggcgatataa gcaaagccaa ccatatctac
    14521 ctatttgcaa ctcaaataaa ccgatacaac gccgtggctg gttacatggt aaacgcatcg
    14581 aaaaaaccgg tgaccgtaat tgccaaggaa gaatcgaacc tgtccaatag gtcaaaagag
    14641 tttataaagg tagactcata ctaccatttc attaaagcgg ttaaccatta cctactaagt
    14701 ggtaacaagc aaaatatggt gtttattggc gaccactgtg aaggttttgg cgcttacagt
    14761 gcaaatttac ttctggaaga ttacgcaaac ctttgcgaac aggctggttg cgaacggagt
    14821 gttattgagc gttttgccga tgaattcaat aacgattatg gcgcactgtt aatttttgcc
    14881 gaagccgagg tttccgataa taccgctgct gaaattatga acttaatgct gattacaggc
    14941 aaattgggca aaacagctgg cggcttgata gctttgaaac ccaaaaataa ttcacatggg
    15001 ctatcggaca tgggtttaaa ccccaattgg ggattaggct tacaggatat aactaaccag
    15061 gaatttgctg acaagttcaa gagaaaatgg ggggttgaaa gtctaccgag caaaaaatac
    15121 aacctggttg aacttttgga aaaaggaagt ttaacaaata ttctcattgt aggcgaagac
    15181 ccaattggaa ctgccaagca taaagcggcc gtgaaggatt acttacaaaa agtaagattt
    15241 aaggtggtaa tcgatgcgtt tttaactgaa acagcccaaa ttgccgatat aatccttcct
    15301 gcttcgcttt ggtttgagag tggaggttcg ttcaccaaca ccaaccatac catccagcag
    15361 tttgaggtag gtgtaaaacc taaggttgag ttaacaactg ccgatttact taccaagctg
    15421 ctgaataggt ttggggtgaa tagcccaacc gatatttacg atatccaaac cgaaatacta
    15481 tcgcttctgc cgcagactga atccggcaaa catctacagt ttatacaaac caccagcgag
    15541 gggcaacacc ttcaattcca tgcaggttgc agctatttga tgcacaactt tgatgttgaa
    15601 tttgaaaaca aattgattaa cgcaaaaacc catagccatg aaagagtttt ccaacattaa
    15661 tgacattttg gactttgcca tagcccgtga acaggatgcg gttgattttt acaaccggtt
    15721 ggccgcaaaa gcacggaaca acgatatgag gcatatattt gaacagtttg cccgcgagga
    15781 aatgatgcat aaggcacgac ttgccaagat aaaggaagag ggagttatta ccctcccaac
    15841 cgagaaagtg gttgacctta agattgccga ttacgttgtg ctcgactccg aacgcgacga
    15901 catgacctac gagcaggcac ttgttctggc catgaaacgc gaaaaggcgg cctttaagct
    15961 ataccttgcc ctttctgaaa aagttgataa aactgagtac aaagaactat tcaagcagct
    16021 ggcacaggaa gaatcgcgcc ataaactacg ttttgagttg gaatacgatg aatacgttct
    16081 aaaggataac taaaacccat ccctaaaagc cccctggcgg ggctttttta attattttcg
    16141 tcacatatct ttgaaacttt atcaaattca ttaatactaa tgaattccca aatggcataa
    16201 caaacaatta agcaaaccaa tttgaaattc aggatacatc tgctatgtaa acaaaaagct
    16261 acaaattcac tatcactatt agtactaaat accgaaaaat aaacatcaac acaaagtatt
    16321 catacctctc attatcaaca cataacacaa ccaatcactc caaaggataa aaaaaacaaa
    16381 aaagttcgat ttatattttg aaaagcatta ctatcaccgt atatttagct tgttatacaa
    16441 ataccattgt aaagggaaat ggattccgac agcaaaattg caagcatgtc atttgcaagg
    16501 gtttaccccc tttaccttac caagattgaa aggaaagggc gaactcgcga ggagctaaac
    16561 gttatcatta aatggcttac gggttttgac actgctacgc tggaaagtct cattgagcaa
    16621 aatgtaacct ttgaggagtt tttccggctt gcaacaatca accccaatgc gggattaata
    16681 accggaaagg tttgcggaat aaaggtagag gaaatcaaga atcctttaac ccaaaaaata
    16741 cgctacctgg acaagttggt tgatgatttg gcaaaaggga aaagtataga aaagataatc
    16801 ccaaagccat aaacaatact tattttcaca atctatcatg aaaaggaact attaccggtc
    16861 caacttcatt ctatctgttt cggcggcggt tctaactgcc ataatcttta ttttccttta
    16921 ctataccatt ggtgttcgcc aacgtagatt tatttacaac gactccaagt ggattgccat
    16981 tcagcaatcc aaaaatgctg cgagtcaggt tgaatcgtat ttcatcaatg ccatacactc
    17041 cgcccgttcg ctagtaaagc aaacgaaggt tttatacgaa aagagagccg acaggtcgga
    17101 gattgtgagt cttatacata gttacacaaa ggataacccc gattttttag cggtttggac
    17161 tatgtgggag ccaaacgcat tcgatggaaa ggataagcaa taccgaaaca ccccacccta
    17221 cgataaggaa ggacatctat cggttacttt tttccgcatg gccgacagca ttgtgtttga
    17281 gtacactgaa cccgaagatt acaatgagtt ttactacact attcccaaag agaggaatag
    17341 cgaaatggtt ttagaaccat acctatatca atatcaggga tacccaattg aattctacga
    17401 aacatcaatt gtagtaccaa taataatcaa aaataagttc tgcggagttt atggtattga
    17461 cctcgacctt aactacctac aaagaaaatt atcggaaata aaactgcaaa ccaaaggtca
    17521 cgtgtcgcta atttcatccg atggcgaaat tgttactcat ccggacaccg cattcattgg
    17581 caaaaatatt ttctcgcttg ctgggactaa cgataccctt gccaggtaca tcgtgaaatc
    17641 ggggctggag cagaactatg aaactgtttg caactttact aaaagtgagg tttttagaac
    17701 attctacccc attcgtgttg gtaagagcga cacccagtgg tcaataatgg ttcaattgga
    17761 taagcgtgag gccaccctac gttcaattta cctccataac attgccctcg gccttttaat
    17821 tgctggcttg atacttattt cctatttgat ttacaacatt ttcgacaggc taaggtacga
    17881 gcatcagctc atattagcta aggaaagtgc tgaacagaaa gagcgcgaac tccaaaaaat
    17941 gtactatgaa ctgcaaacat ccgaagagga gctacgggca accaacgagg aacttttcgc
    18001 aacatccgaa acgttacgtg agagcaacct tatgcttacc ggagctaagg aaaaagccga
    18061 ggaggccagc agactcaaaa cagcatttct tcataaccta agtcacgaag tgcgaacacc
    18121 cttaaacgca atttatggtt tctcgcaaat gctcaataac attgatatct cagcagacga
    18181 atctaagaag ttcattcaaa tcatacagga ctcaagcaag caattagttt ctatcatcag
    18241 cgatatcata acaatttcat ccattgaaac cggacaggag cgaataacca acactaacat
    18301 acacctgaat accctgctcg atgagcttta ccaaactttt tctgcaaaac ttgccaataa
    18361 accagttgaa ttgagggttt acaaggagtt ttccgataat gaagatgaaa tttttgtcga
    18421 tgacctaaag cttagacaag tactacacca cttgctttta aatgcagtga agttcaccca
    18481 caatggctct attgaatttg gttataggct taaaagtagc aacctggaat tctacgtcaa
    18541 ggatactggc ataggaattg aaggtaaaaa gcaagatgtg atttttgaac gctttaccca
    18601 ggccgatgaa aatatccatg ctgagtatgg gggaaccgga ttgggtttat caatatgtaa
    18661 gggcttttta aacttaatgg gcggtgctat ttgggttgaa tcagagctag ggcaaggggc
    18721 aaccttttac tttactctac catatcaccc aataaccagt aaagagagta aaggtattag
    18781 tatccaatca caaacgaaca tttccgaaat gaaaacaatt cttgtggccg aggacgatat
    18841 aaatagcttc aagctgctaa aagcggtgct aaaagatttt gactttcacc tcctacacgc
    18901 cgataatggc cgcaaagctg tagagctttg ccaaaacgaa gattcaatct gcctggtact
    18961 catggatatt aaaatgccgg agctcaacgg agtattggct actaagctca ttaaagagct
    19021 aaaacctact ctacccgtaa tagcccaaac tgcttatgcc acccttgacg atattgaaaa
    19081 gaacagggag gtgttcgatg cctatatcac aaaaccctta gatattgaac agctaaaaaa
    19141 aataatttta gagttttgcg atagattcgg gaaaagcaaa aagtattaat cctaagttct
    19201 ttgtattaac taattctact gggctaactt taggggaatg agctggaatg attcggaaca
    19261 atcagaatat gatgaagtta gaggaagttt gacttcgtta aacgtgaaaa gtgaaaagtt
    19321 aaaagaggaa ttgagaatat gaaggagtta gaggaagtta gaggagaatt gagcagataa
    19381 atatatcata gattttcatt gcaaatgcaa cttttttgtt aaacaaaatt tcttccagtt
    19441 tttctttggg agttagaaac cccaaacgtt ttcttggtct gttgtttagg atctcttcca
    19501 ctcatttaac ctgttcatcc gtgatgtttt caaaggatgt ttttttggga aagaactgcc
    19561 tgatcagccc attggcattc tcgttagccg ggcttttccg ttgcgatgca acgctttccc
    19621 cttgctcttt ggaaaattta atgcgtgcat cgctatacat gtgggaataa tcatgttgtt
    19681 acaagtatta cgcccctacg gggctggaaa cgttgttagt aactcgttat tcatgaaatt
    19741 gacctcaccc ctcccctctc ctgacaggag aggggagaag caataaggaa accttattgc
    19801 agggctaaag tctcccctct caggggagat ttagaggggt catctaggtt gaatgtggaa
    19861 aacaacctca cccaaccatt ttccttaagg ggggaatgga gcggaagaag caagcagagg
    19921 cataaaagcc tgacccggca atgggtcggg ggtggaacgt ggcggtgggc ctagtaagcg
    19981 ataccatatg agccacgctg caggcgtggc gaatagtatc gcgtggcagt tccacccgcg
    20041 gggtacccat cgggtcgggc ttttcagcct tgatcttctt tggttctttc ttgtatcaag
    20101 acaagaaaga acatgtaaac c
//
LOCUS       DAOSAL010000047        19740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_1941_length_19740_cov_55.571806, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000047 DAOSAL010000000
VERSION     DAOSAL010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 19740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19740
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1941_length_19740_cov_55.571806"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..202)
                     /locus_tag="PK049_07540"
     tRNA            complement(129..202)
                     /locus_tag="PK049_07540"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(166..168),aa:Arg,seq:tct)
     gene            complement(269..928)
                     /gene="hisIE"
                     /locus_tag="PK049_07545"
     CDS             complement(269..928)
                     /gene="hisIE"
                     /locus_tag="PK049_07545"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005780060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HOK61449.1"
                     /translation="MTTLNFDKLNGLLPAIIQDAATGKVLMLGFMNREALEVTRKTGL
                     ATFYSRTRRQIWTKGETSGNFLKVTEIIPDCDGDTLLVKVTPQGPVCHTGNDTCFNET
                     NGNTLGFIDYLEEIIENRKQASADQSYTAKLLAAGTKRIAKKVGEEAVELALEAENGT
                     DERLLDEAADLIYHTLVLLSSRNLKFSKVIETLRTRHTDSKVSNSEVEDFKRGEKQWG
                     K"
     gene            complement(943..1698)
                     /gene="hisF"
                     /locus_tag="PK049_07550"
     CDS             complement(943..1698)
                     /gene="hisF"
                     /locus_tag="PK049_07550"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOK61450.1"
                     /translation="MLAKRIIPCLDIKNGRTVKGVNFENLADAGDPVYLAEKYYNEGA
                     DELVLLDISATLEGRETFIEVVRNVAKAIAIPFTVGGGISSVDQAERLIKAGADKISI
                     NTAAIQKPELIGKMAYAFGRQCVVVAIDTMLTDKGWEVVSHAGTKRTGIKALEWAKTC
                     EILGAGEILLTSHTSDGTREGFAVDITRKISQSISIPVIASGGAGLPEHFAEVLTTGM
                     ADAALAAGVFHYGVLSIPQVKKYLQSRNIEVRY"
     gene            complement(1680..2396)
                     /locus_tag="PK049_07555"
     CDS             complement(1680..2396)
                     /locus_tag="PK049_07555"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HOK61451.1"
                     /translation="MLVVPAIDIIDGKCVRLFQGDFLRVKEYDLNPVDQAKVFADMGL
                     EHLHLIDLDGAKDGAPRNLRVLEQIVKSTSLKVDFGGGLRDLNSVNLAISAGAWKVNL
                     GSMLLQANEWFMNDIPTQKVIAAVDCEHFMVKTNGWQNQTRVRVDELLNRLLSLRIEH
                     FTITDIGRDGTLTAPAFSLYEDLRQKFPDITIWASGGVSSVADVINLKKMAINGAIVG
                     KAYYENKINLKELLQCLQNE"
     gene            complement(2390..2986)
                     /gene="hisH"
                     /locus_tag="PK049_07560"
     CDS             complement(2390..2986)
                     /gene="hisH"
                     /locus_tag="PK049_07560"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOK61452.1"
                     /translation="MKTNITIGIIQYGAGNQASVMNALDHLGVKNIPIVTPLDISKAD
                     KLIIPGVGHAKRAMEQLKETGLDAALQEATIPLLGICLGMQLLGEKSEEGDATCLRLC
                     SFNTVKFNLSLKIPQMGWNKVTLGSNPLFSGLPQEEWFYFVHSYYVSKNQYTIATCNY
                     GIEFTAAVQHKNFWGVQFHPEKSGKVGLQVLKNFVELC"
     gene            complement(2989..4071)
                     /gene="hisB"
                     /locus_tag="PK049_07565"
     CDS             complement(2989..4071)
                     /gene="hisB"
                     /locus_tag="PK049_07565"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005846279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HOK61453.1"
                     /translation="MKPILFIDRDGTLIKEPTDYQIDSFEKLDFMPGVFSWLGRICKE
                     FDFYLVMVTNQDGLGTSSFPEDGFLGPHQVMLRALNAEGIQFDEILIDRSFPDENLPT
                     RKPGIDLVKHLLNDDFDIANSFVIGDRETDELFARNIGCQAIRLGTHATIPGSFTAMG
                     WEEVYNLVKPKRLAAISRKTNETDIAVNINLDGTGQAKVNTGIGLFDHMLEQLAKHSG
                     IDIDIVCLGDLRVDEHHTIEDVGITLGEALRKALGDKAGISRYGFALPMDESRAMVLL
                     DFGGRPYLVFEGSFSREFVGQMPTEMVKHFFHSLAYSAQVTLHIDFDGENDHHKVEAI
                     FKAFARSLGMAIKKDGNSIPSTKGII"
     gene            complement(4068..5129)
                     /gene="hisC"
                     /locus_tag="PK049_07570"
     CDS             complement(4068..5129)
                     /gene="hisC"
                     /locus_tag="PK049_07570"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004290345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOK61454.1"
                     /translation="MMTLSETLELVRPNIMRLKPYSSARNEFTGGNAILLDANENPYN
                     LWNDDLIVNRYPDPLQREAKLKIAEIKGVSPEQIFLGNGSDEAVDLIIRCFCEPSTDS
                     IVIFSPTYGMYSVCAEVNNVQVKEFILNNDFSINLQQFFNGISPSTKVVFICSPNNPT
                     GNAQPIETIEQILSKFRGIVVVDEAYIDFCPEKTYLSLLNRYPNLVVLQTFSKAWAMA
                     GARIGIAFANPKIIATLNKVKFPYNIGVPSLSVLSRAIEQYSNTKSLISQIVSERELL
                     RLRLQQLSCVKMVYPSDSNFLLVKTTDANSVYNYLLNNGIVVRNRTHEPLCNNCLRIT
                     VGKPYENSKLIQILNTYRP"
     gene            complement(5134..6411)
                     /gene="hisD"
                     /locus_tag="PK049_07575"
     CDS             complement(5134..6411)
                     /gene="hisD"
                     /locus_tag="PK049_07575"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOK61455.1"
                     /translation="MVTITDKTNINYSELLKRPVEYNPEIRTTVQEIIEKVRVQGDSA
                     LRLLTKKFDGIDIDRFEVDEQTIASSRNQVGENLKAAIEIAARNIERFHAAQKVSDVE
                     VVTMPGVSCKLVYRPLERVGLYVPGGTAPLISTVLMLGVPAKVAGCNELIVCTPPPVN
                     PIILYACSIVGAKVFTVGGAQAIAAMAFGTETIPRVDKIFGPGNSYVTEAKMQVSAMG
                     IPIDIPAGPSELLVIADESAIPQFVAADLLSQAEHGADSQVVLISNCNSIINETLDEI
                     NKQIKNLPRMDIALKALENSYAIKVETVERAIEISNTYAPEHLILAVASPEKYETMIK
                     NAGSVFMGNYTPESAGDYASGTNHTLPTSGFARSWGGVNLLSFTKTITYQSITPEGIQ
                     ELGNCIVEIANAERLTAHANAVKLRMEKLSNQS"
     gene            complement(6423..7283)
                     /gene="hisG"
                     /locus_tag="PK049_07580"
     CDS             complement(6423..7283)
                     /gene="hisG"
                     /locus_tag="PK049_07580"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005789131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOK61456.1"
                     /translation="MNTKITLAIQKSGRLNEGTIELLNRCGIRISNGNNHLKALAEGF
                     PMEVLLLRDDDIPQYVADGVADIGILGYNEVLEKGKDIEVIRELGFSRCRLSIAVPRD
                     VNYSGRQWLSGKRIATSYPEILKTFLNQNAIEADIHTISGSVEITPGIGMADAIFDIV
                     SSGGTLLSNNLKEVDVIMKSEAVLVANKSLDEQKSELLKKLEFRIEAVRKAQRNKYIL
                     LNAPKDKLNQIISLIPGMKSPTVLPLAIEGWCSLHSVVNEDDFWEVIGRLKDAGAEGI
                     LVVPIEKMVI"
     gene            complement(8115..8297)
                     /locus_tag="PK049_07585"
     CDS             complement(8115..8297)
                     /locus_tag="PK049_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61457.1"
                     /translation="MGTPRVELPRDTIRHACSVAHMVSLTRPTATFHPRPIAVSGFYA
                     SACFFRSIPPQGKGLG"
     gene            complement(8713..8895)
                     /locus_tag="PK049_07590"
     CDS             complement(8713..8895)
                     /locus_tag="PK049_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61458.1"
                     /translation="MGTPRVELPRDTIRHACSVAHMVSLTRPTATFHPRPIAVSGFYA
                     SACFFRSIPPQGKGLG"
     regulatory      9325..9513
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            9618..11585
                     /locus_tag="PK049_07595"
     CDS             9618..11585
                     /locus_tag="PK049_07595"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOK61459.1"
                     /translation="MYFRVGVILYAFAISGITAARAQMCLEDTIRVEEVTIYSTYRQL
                     HTIGTNTIKIDTLRLTNFEGKSLTELLQSTGVNIRSYGVGGLATIAVRGGGSSHTAVV
                     WNGINLQSPLNGGVNLSQFPVSLFSSVTVQHGGNGTVYGSGAVSGIVILENGGLLLQP
                     NGARAGFTYGSGNTRGANASVKIGNSKSALSLKYSGLIADNDFEFINTYKFGNPRERI
                     TNAQAAQHGILADAVLRFDNNALWNISGWFTHNDKNVQTLMSSSQISQANQLDNNFAV
                     SSNLTGNLPFFSLKLRNAFINGDNHFTDPASGISSLNTYKQLVNEIELKKTMLKHQEL
                     VVGLGYSVDIASSQSYTENALRNRISAYASLVSHLLGSRIKLVLSLRDELVDGSLIPL
                     VASSGLEYKATKSLMLRASAASSYRLPTLNDLYWSSTTYAVGNPNLEPEYGWNTDLGF
                     DYTIIQNALNLKFSGSYFISRLNRWIVWLPDAQDNNRWKPSNISTGKSEGFESFIILG
                     VKLMPISIRADLSYTLTNSKIYDSGSYNGKPMIYIPRHKVGASISIIYGQFNFGYNHS
                     FTSERYTDELNTLPLYNLGDIYMGYSCKAWGSAFTIGVGVNNVWNEQYQLVRNYAMPL
                     RNYFIRLNIDFQSKSTNIINLKTFKNEQKIF"
     gene            11566..12639
                     /locus_tag="PK049_07600"
     CDS             11566..12639
                     /locus_tag="PK049_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61460.1"
                     /translation="MNRKFFNLTIFAFLAFAFFSCEKDDKKPGKFENGAIVLNEGSFM
                     QSNASVSWVSYQSDSVVNNVYENVNARPLGDVLQGGVKANGKIYLVLNNSGKVVIVNS
                     SDFIQTGEVSNLNNPRYAVVVDGKLFVSQWNGWGQKGAVMVYNANGSEKIDSIPVGTG
                     AEGIIYAAGKVWVANSGGYGVDSTVTVINTSTLQVEKTINVGYCPKEFTVDANGKVWV
                     LCSGTTIYDANWNVAGHKPSSLAAINTSTMSVEKSVNLFNDMHPSHIDISVDKQTIYF
                     GGGFGFNGIYNVQIVASSISQTPLVSGYFYGFNVNPINGDIYTLEAPNFTSAGSLKVY
                     NSDGSGPKKTASVGIGPNGVLFN"
     gene            12648..13340
                     /locus_tag="PK049_07605"
     CDS             12648..13340
                     /locus_tag="PK049_07605"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HOK61461.1"
                     /translation="MKCLMSWSGGKDSALALYRCINDHNHNIVGLVTTINSSTNRVSM
                     HGVALDVLKYQADAIGIPLHVITLPEKCSMDEYESIMGAFMSRMKQDGVEGVVFGDIF
                     LEDIRKYRERQLEGIGITPIFPLWGLASAEVASQFLDNHFKSIITCVDSDLLDESFVG
                     QFYTSAYIEKLPGGVDPCGENGEFHSFVTAGPIFQKPIEVKVISKIRKEYPNPQGGNP
                     KTFWFADIAASK"
     gene            complement(13330..14139)
                     /locus_tag="PK049_07610"
     CDS             complement(13330..14139)
                     /locus_tag="PK049_07610"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HOK61462.1"
                     /translation="MKKILLILLLPIGLFTNAQEITKVENAVGRQVIAGSISEDEARA
                     KAIAQAKVDALRKAGVTEHIQSYEMLYKSEVGNKFDEVFMSDMFSEIRGAVRKYNIVK
                     IDKGIDEFKNFYVEAIIDAEVIQYTTAKDPAFQVSIDGIKQGYQSGERMRYTVTPTQD
                     CYLNIFNLYENNASLIYPNQFEKSRLFKAGEKVTFPLSELIDGYTLEKSAKEPETNKL
                     VFVFTKDNIPFIKYKLNQEGDQLTTFEDISAWLFSISPDRRVNYFIQFVIY"
     gene            complement(14268..15470)
                     /gene="trpB"
                     /locus_tag="PK049_07615"
     CDS             complement(14268..15470)
                     /gene="trpB"
                     /locus_tag="PK049_07615"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008664373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOK61463.1"
                     /translation="METIISNIQHEFVQPLSNMLKYNGRYGEYGGAYVPDVLAKPLER
                     LANEFDRLTKDSSFQKEFVQLLKHYVGRPSPLYRAKRLSEQVGSTIYLKREDLNHTGA
                     HKINNTIGQVLVAKRMGATEIIAETGAGQHGVATATAAALMGIPCKIFMGAKDAERQA
                     LNVRRMKLLGAEVITTTMGTATLKDAVDAALMYYIEHPESFYLLGSQVGPHPYPRMVG
                     YFQSVIGNEAREQILMQEGRLPNAIFACVGGGSNAIGLFSGFLNDVEVEIYGAEGGGE
                     AIPGNTAATLTFGKPTVFQGTLSYCLVDDNGNPCESHSVAAGLDYPGISPQHAYLKDT
                     SRAKYFAVTDTEAIKAFEMLSQLEGIIPAIESSHAVALAAKMLKNKNQLAIVNLSGRG
                     DKDVEREF"
     gene            complement(15825..16280)
                     /locus_tag="PK049_07620"
     CDS             complement(15825..16280)
                     /locus_tag="PK049_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61464.1"
                     /translation="MKKILIALTLFVAGTNLLLAQNINYDPELAKKLNADEYGMRLYT
                     FVLLKSGEKQNLSKSETDSIFRGHMNNIIRLENEGKLFVAGPFGANPYGYRGLFIFYE
                     TDINKVEEMVNTDPAVSSGILKPEIFNWYGSAALPIYLDYHNKIWNKMP"
     gene            16349..16951
                     /locus_tag="PK049_07625"
     CDS             16349..16951
                     /locus_tag="PK049_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61465.1"
                     /translation="MKRITFCIGLSVMSLLGYGQIGNEILTSIALGKYSVALEQINEL
                     PPSPELIQFKAIAFGGLNMWDSAVVYYSKLTNIDTQLAKKGLARCYESVGNSSKAIDL
                     YKQILADDSLNASIITRYANLLRSSSLHNQALEQFQKLIELDSTCAKYWEMLGDQYKA
                     MNMTVESTTAYNKAYLLNSNNLFVASKYFAQLIKSNVPLQ"
     gene            16952..17641
                     /locus_tag="PK049_07630"
     CDS             16952..17641
                     /locus_tag="PK049_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61466.1"
                     /translation="MYENILDAYQVDSTYMPILMLKGKVESDMKKYSQAEITFTRIAS
                     LGDSSFFTLKNLAIAKHSNGSYLEAENLFIKAYEMDSTDFLLNFVYAKTLQNVGERNL
                     ALDIISNTIKLLYPPDELVASFYELAGDIYNSGNKIDLAVENYQMALEKDKKNELKYL
                     NKIIWCKIYDKKFRDAEYLVHKYDSIAQEKYADDSIQLKNEQRKTGFYKDLLKKEFFF
                     MDDLKNGKFLV"
     gene            complement(18057..18758)
                     /gene="sfsA"
                     /locus_tag="PK049_07635"
     CDS             complement(18057..18758)
                     /gene="sfsA"
                     /locus_tag="PK049_07635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HOK61467.1"
                     /translation="MKFDQPLIHGRLVKRYKRFLADILLDNGNVVTAHCTNSGSMKSC
                     LEENAEVFISPSSNPNRKLPYTWEMIRINNGWVGINTSNPNRIAYDILKNHWIDELPT
                     FKTLIREVKYDDSRFDIYGESENQRWFFEVKNVTLKEGRFAQFPDAVTTRGQKHLSTL
                     IKAKAEGYHVVMIYIIQRTDVDIFAPAWSIDSAYAKELIKAFDAGVKLFAVQVNVTPE
                     SIEPLRLIPIDLIQK"
     gene            complement(18804..19529)
                     /locus_tag="PK049_07640"
     CDS             complement(18804..19529)
                     /locus_tag="PK049_07640"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HOK61468.1"
                     /translation="MCFTLSVRVEKHRLEKVMNARFREDFRFEPFYFVSAFDFPVIPV
                     VASGNPEYFTPMSWGLIPNWVKNPIEAQSIKEKTLNARFESILEKPSFRYSAKSNRCI
                     VPATGFFEWQHIHGKKIPWFIGLNNNEIMHLAGIYSTWTNTETGEMVDTFSIITIPAN
                     PLLEKIHNTKKRMPAILKPENAEKWLDKDLKTSLYSDIIKPVEQEYLKAYTVSPIISN
                     PKAKRNVPEVLEPYSYPEQQSLF"
BASE COUNT         5385 a         4077 c         4176 g         6102 t
ORIGIN      
        1 tgagtttttt caagtgatcc gggcgggatt agttcgcctt cggctcatga tcccgccctg
       61 atgtttttct aagaatttaa ggctatcctt gttcagaata gcctttggta aatatgagtt
      121 ttttcaagtg atccgggcgg gattcgaacc cacgacccac agcttagaag gctgttgctc
      181 tatccaactg agctaccgga ccggcttcga tatttcgaag cgcaaaatta atcattactt
      241 tcaaaaaata aaacaccgta catcattttt acttgcccca ctgtttttcg cctcgcttaa
      301 aatcttcaac ctcactgttg ctcacttttg aatcggtgtg ccgggttctg agtgtttcaa
      361 taactttact gaatttaagg ttacggctcg atagcagaac cagggtgtgg taaatcaaat
      421 cggcggcttc gtcgagcaac cgctcgtcgg taccattttc ggcctcaagc gcaagctcaa
      481 cggcttcctc gcctaccttt ttggctatgc gtttggtgcc tgcagctaaa agtttggctg
      541 tgtagctttg gtcggcactt gcttgcttac ggttttcaat tatttcctca aggtaatcaa
      601 taaagcctaa ggtgttacca tttgtctcat tgaaacaggt atcgtttcct gtgtgacaca
      661 ccggcccttg tggtgtaacc tttaccagaa gcgtatcgcc atcgcaatcg ggaattatct
      721 ccgtcacctt aagaaaattt cctgaggttt cgccttttgt ccatatttgt cggcgtgttc
      781 tgctgtagaa tgtggcaagc cccgttttac gggtaacttc aagtgcttcg cgattcataa
      841 acccaagcat aagcacttta cctgtggctg catcctgaat aattgcagga agcaatccat
      901 ttagtttgtc aaaatttagt gtagtcatag catctgtttt tattagtatc taacttcaat
      961 atttctgctt tgtaagtatt tttttacctg tggaattgaa agtacgccgt aatggaacac
     1021 gcctgcagcc agggctgcat cggccatacc tgtggttaat acttctgcaa aatgctcagg
     1081 caatcctgca cctcccgatg caatgacagg aatactgatg ctttgggaaa tttttcgggt
     1141 gatgtcaact gcaaaccctt cccttgttcc atcggatgtg tgtgaagtga gtagtatttc
     1201 accagctcca agtatttcgc aggtttttgc ccattcaagg gcttttatgc ccgttctttt
     1261 tgttcctgca tggcttacaa cctcccagcc tttatcggta agcatggtat cgatggcaac
     1321 aaccacacat tgccttccaa aggcataggc cattttgcca ataagctctg gtttttgaat
     1381 tgcggcagtg ttaatggata tcttatcggc gccggcttta ataagccttt cagcttgatc
     1441 tactgaactt attcccccac caacagtgaa aggaatagct atagctttag ccacattgcg
     1501 cacaacttca ataaaggttt cgcgcccttc aagggtagcg gagatatcaa gcagcactag
     1561 ctcatcggct ccttcgttgt aatacttttc ggccagatat actggatcgc ccgcatcggc
     1621 aaggttttca aaattcacac ctttaacggt tcggccattc ttgatgtcga ggcatggtat
     1681 tattcgtttt gcaagcattg tagtagttct tttaggttaa tcttgttctc gtagtatgct
     1741 ttaccaacaa tagctccatt tatggccatt tttttaaggt taataacatc agctacagag
     1801 ctaacccctc cgctagccca aattgttata tcggggaatt tctgtcgtaa atcttcgtaa
     1861 aggctaaaag cgggtgcggt taatgtacca tcgcggccta tgtcggtaat agtgaaatgt
     1921 tcaattctca aacttaatag gcgatttaaa agctcgtcaa ctctcaccct ggtttgattt
     1981 tgccacccat tagttttaac cataaagtgc tcacaatcca ctgcggctat aactttctgg
     2041 gttggtatat cgttcataaa ccattcattg gcctgtaaaa gcattgaacc gaggttaacc
     2101 ttccatgccc cagcgctgat agcaaggttt actgagttta agtcacggag tccccctcca
     2161 aaatcgacct ttagggaggt ggattttaca atttgttcca gaaccctcag gttcctggga
     2221 gcgccatctt ttgcaccgtc aaggtcgata aggtgtaggt gttccaatcc catatcggca
     2281 aatactttgg cttggtctac gggatttaaa tcatactcct taactcttaa aaagtcgccc
     2341 tggaatagcc taacgcattt cccatcaatt atatcgattg ctggcactac taacatagct
     2401 ctacaaagtt tttaagtact tgtaatccta cttttccgct tttttcgggg tgaaattgaa
     2461 ctccccaaaa atttttatgc tgaactgctg cagtaaattc aatgccatag ttacaagtgg
     2521 caatcgtgta ctggttttta gaaacatagt agctgtgaac aaagtagaac cattcctctt
     2581 ggggcaatcc actaaacaaa ggattgctgc ctagcgttac tttgttccaa cccatttggg
     2641 gtattttaag cgataaatta aacttaacag tattaaagct gcataacctc aggcaagtgg
     2701 catcaccctc ctcgctcttc tccccaagga gttgcatacc caggcaaatg ccaagcaacg
     2761 gaatggtggc ttcctgtaaa gcggcatcaa gcccagtttc ctttaactgt tccattgctc
     2821 ttttggcatg tccaaccccg ggaataataa gtttgtcagc tttgctgata tccagtggcg
     2881 tgacaattgg aatatttttc actcctaagt ggtccagagc attcattacc gaagcctgat
     2941 tgccggctcc gtattgaatt atacctatgg taatattcgt tttcatagtt aaataatacc
     3001 ttttgtgctg ggaatactat taccatcctt tttaattgcc attccaaggc tccgtgcaaa
     3061 tgccttaaaa attgcttcca ccttatggtg gtcgttttcg ccatcaaaat cgatatgcaa
     3121 tgttacttgg gcggagtagg caagcgaatg gaaaaagtgc ttaaccattt cggttggcat
     3181 ttgtcctacg aattcgcgag aaaaactgcc ctcgaatacc aagtatggcc taccgccaaa
     3241 gtcaagcaac accattgctc tgctttcgtc catgggtaag gcaaatccgt atcgcgaaat
     3301 accagcctta tcgcctagag cctttcgtaa cgcctcgcca agagtaatgc ccacatcctc
     3361 aatggtgtgg tgctcgtcta cccgtaaatc gccaaggcat acaatgtcaa tatctattcc
     3421 tgagtgcttg gcgagttgct caagcatgtg gtcaaagagt ccaatacctg tatttacctt
     3481 tgcctggcct gttccatcaa ggttaatgtt tacagcgatg tcagtttcgt tggttttacg
     3541 tgaaattgca gcaagccttt taggctttac taggttatat acttcctccc agcccatagc
     3601 tgtaaaacta cctggtatgg ttgcgtgggt tccaaggcga attgcttggc atccaatgtt
     3661 tctggcaaat agttcatcgg tttccctatc gccaataaca aaggagttgg caatgtcaaa
     3721 atcatcattt agaagatgtt taaccaggtc aatacccggt ttgcgtgtgg gtaaattctc
     3781 gtcagggaag gaacggtcaa taagtatctc atcgaactga ataccctcgg cattaagtgc
     3841 tctaagcatt acctggtgtg ggcccaaaaa accatcctca gggaaactgg acgttccaag
     3901 accatcctga ttggttacca ttaccaggta aaagtcaaat tccttgcata ttcgtcctag
     3961 ccatgaaaac actccaggca taaagtccag cttttcaaag ctgtcaattt ggtaatctgt
     4021 gggctccttt attaaggtgc catcgcggtc gataaaaagt attggtttca tggtctgtac
     4081 gtgttaagga tctgaattaa cttgctattt tcatagggtt tacccactgt aatacgtaaa
     4141 cagttattgc ataatggttc atgggtgcgg tttcggacca ctattccatt gttcagcaaa
     4201 taattgtata ctgagttagc atcggtagtc tttaccagca aaaagtttga atccgaagga
     4261 taaaccattt taacacacga caattgctga agtcttaatc tcaaaagttc cctttcgcta
     4321 actatctgac ttatcaacga ttttgtgttt gaatattgct ctatggctct agatagaact
     4381 gaaagcgagg gaacaccaat gttgtatggg aacttaacct tgttaagtgt ggcaattatt
     4441 ttggggtttg caaaagcaat cccaattcga gctcctgcca tagcccaagc ctttgaaaag
     4501 gtttgcaaaa caactaggtt tgggtaacgg tttaagaggc taagataagt tttttcaggg
     4561 cagaaatcga tgtaggcttc atcaaccaca actataccac gaaattttga tagaatttgc
     4621 tcaattgttt caatcggttg agcattgcca gtgggattgt tcggactaca tatgaataca
     4681 actttggtgc taggtgatat accattaaag aattgttgga ggttgatact aaaatcgttg
     4741 ttcagaataa attctttgac ctgcacgttg ttaacctcag cgcaaaccga gtacatccca
     4801 taggttggtg aaaatattac aatgctatcg gttgatggtt cacaaaagca acggattatg
     4861 aggtcaactg cttcatcgct accattgccc agaaaaattt gctcaggtga aactcccttg
     4921 atttctgcta ttttcagttt tgcttcacgc tgcagtgggt cggggtagcg gtttacaatc
     4981 aaatcgtcgt tccaaaggtt gtaagggttt tcgttagcat cgagcaaaat agcattgcct
     5041 ccagtaaact catttcgcgc cgatgagtaa ggttttagcc tcataatatt tgggcgaacc
     5101 agttctaatg tctcacttag tgtcatcatg gtgttaactt tgattactta gtttttccat
     5161 tcttagctta actgcattgg cgtgtgcagt caatcgttca gcgttagcta tttcaactat
     5221 gcaattacca agttcctgaa tcccttcagg ggtaattgac tggtaagtaa tggtttttgt
     5281 gaacgaaagc agatttacgc caccccaact tcttgcaaat ccgcttgtag gaagggtatg
     5341 attggttccg cttgcataat cgcctgccga ttcgggtgtg taattcccca tgaaaaccga
     5401 acccgcattc tttatcattg tctcatattt ttcaggactt gcaactgcaa gtatcaggtg
     5461 ttccggggca taggtgttgc ttatttcaat tgctcgttca actgtttcca ccttaatggc
     5521 atagctgttt tccagcgctt ttagcgctat gtccattcta ggtagatttt taatctgttt
     5581 gttaatctca tctaaagttt cgttaataat tgagttgcaa ttgcttatta gcacaacctg
     5641 gctatcggcc ccatgctctg cttgcgatag gaggtcggca gcaacaaatt gtggaatggc
     5701 actttcgtcg gctattacaa gtaactccga tggtccggca ggtatatcaa taggaattcc
     5761 catggcagaa acctgcattt ttgcttcggt tacatagcta ttccccggcc caaaaatctt
     5821 atcaaccctt ggaatggttt cagttccaaa tgccatggca gctatggctt gtgcaccacc
     5881 aactgtgaat acctttgccc caactatgct acaagcataa agaattattg ggtttactgg
     5941 tggaggagtg caaacaataa gttcattgca tccggctacc tttgcaggga cacccagcat
     6001 tagcactgtc gatattagtg gggctgtccc acccggaaca taaaggccta cgcgttccag
     6061 gggtcggtaa acaagcttgc agcttacacc gggcatagta accacttcaa cgtcgcttac
     6121 tttttgggct gcgtgaaacc tttcaatgtt ccttgcggct atttcaattg cagctttaag
     6181 attttcccca acctgattgc ggctacttgc aatggtttgt tcatcaacct caaacctatc
     6241 gatatcgata ccatcaaact ttttggtcag caaccgtagg gcagaatcgc cctgaaccct
     6301 gaccttttca ataatttcct gaacagtagt tcttatctca gggttatact caaccggtcg
     6361 cttaagtagc tcagagtagt taatgttggt tttatcagta attgtaacca tagctttagc
     6421 ttttaaataa ccattttttc gataggcact acaagtattc cctcggcacc tgcatctttc
     6481 agcctaccaa taacttccca aaaatcatcc tcgttgacta ccgagtgaag tgagcaccac
     6541 ccctcgatag ccaatggtaa aacggttggg cttttcatcc caggtatcag gctaatgatt
     6601 tggttcagct tatcctttgg agcatttagt agtatgtact tgttgcgttg tgctttacgc
     6661 acagcctcaa ttcggaactc aagcttttta aggagttcgc tcttttgctc atctaagctt
     6721 ttattggcaa caagaacagc ctcgcttttc attataacat caacctcttt gaggttgttg
     6781 cttagcaatg ttcctccgct acttacaata tcgaatatgg catcggccat tccaatcccg
     6841 ggcgttattt ctactgaacc gctaatggtg tgaatatcag cttcaattgc gttttgattc
     6901 aggaatgttt taaggatttc aggatatgat gttgctatcc gctttcccga tagccattgc
     6961 ctgccactat agtttacatc gcggggaaca gcaatggaaa gccggcatcg ggaaaagccc
     7021 agttcacgga taacctcgat atctttccct ttctcaagaa cctcgttgta tccaagaatg
     7081 cctatatcgg caaccccatc ggcaacgtat tgcggaatat cgtcatccct tagcagtagt
     7141 acttccattg ggaaaccctc agccaacgct ttaagatggt tgtttccgtt tgaaattctt
     7201 attccgcatc ggttcagtag ttcaatggtt ccttcgttta atcggccgct tttttggatg
     7261 gccagagtaa tttttgtgtt catatcaact tgtttttggt gatttactaa atgcagaaag
     7321 ggtctgcctt ttgaatggca gacccttgaa taaggtagtc tctttatcga tttactattg
     7381 gttataccaa atgctcatct gccattcgcc agaagtgtga atggtgatga tgatgaagag
     7441 tggtataact agtagctatc atttgcggtt tgttgttctg caaataaaag taacttttat
     7501 taaaaatcca aatcagcata gaaaaattaa tgaaacacca ttggaatgta gcttgtagaa
     7561 aaaatgtgat tcaatgttat ttagttaagg ttatttgtca tgttgcgcac aagcgaaaca
     7621 tcacgctgtc gtgctgagtc ccgatttata gggattggct tcgccgaagc atcacattgc
     7681 tcatttttct tcaatccttt gaagagatcc ttcaacatag ctcgggatac taaaaaccct
     7741 atcttccttt ttgctcactt gtcatgctga gctaggcgaa gcatctcttt gccttaaacc
     7801 ctctaatact tgttaatttt ttggaaactg ctctttgcgg tgtttttata ttgcgatgca
     7861 atgcctcacc cttgctcttt ggcatatgca ttgcgtgcat cgctatacat atgggaataa
     7921 tcatggtgtt acaaatatta cgcccctacg gggctggaag cgttgttagt tgttcgttgc
     7981 tcgtgaaact attgtcatgt tgagcgtaag cgaaaaatct catggggccg attagagatc
     8041 cttcgctacg ctcaggatga caaataaaat tattcgtgta agttgctcgt tgttctttga
     8101 tcgtaaaatt gacctcaccc cagccctttt ccctgagggg gaatggagcg gaagaagcat
     8161 gcggaggcat aaaagcctga cacggcaatg ggtcgggggt ggaacgttgc ggtgggtcta
     8221 gtaagcgata ccatatgagc cacgctgcag gcgtggcgaa tagtatcgcg tggcagttcc
     8281 acccgcgggg tacccatcgg gtcgggcttt tcagccttga ttttctttgg ttctttcttg
     8341 tatcaagaca agaaagaacg tgaaaataat tctttgtaat tatttttctt ttgcgatgca
     8401 acgcttcagt gttgctctct ggaaaaggtg ttgcgtgcat cgcaaaataa tttgaacacc
     8461 tcaataccct gggttaaaac cctgggttaa tgtgatttgc cctttcaggg cgaatgatgc
     8521 acgcgtcggg atacgtgcgc aaacgagagg gctggaagcg ttgttagttg ttcgttgcac
     8581 gtgaagttat tgtcatattg agcgtaagcg aaaaatctca tggggccgat tagagatcct
     8641 tcgctacgct caggatgaca aataaaatta ttcgtgtaag ttgctcgttg ttctttgatc
     8701 gtaaaattga cctcacccca gcccttttcc ctgaggggga atggagcgga agaagcatgc
     8761 ggaggcataa aagcctgaca cggcaatggg tcgggggtgg aacgttgcgg tgggtctagt
     8821 aagcgatacc atatgagcca cgctgcaggc gtggcgaata gtatcgcgtg gcagttccac
     8881 ccgcggggta cccatcgggt cgggcttttc agccttgatt ttctttggtt ctttcttgta
     8941 tcaagacaag aaagaacgta aaaatggctc tttgccttgc tttcccttag cgatgcaaag
     9001 tttcagtatt gctctttgtg aaaagtactg cgtgcatcgc cttacatatg ggagtattca
     9061 tagggttaca aatatttcgc ccctacgggg ctggaagcgt tgttagttgt tcgttgctcg
     9121 tgaaactatt gtcatgttga gcgtaagcga aacatctcat ggggtcgatt agagatcctt
     9181 cgctgtgcgc aggatgacag gcatgtgtat ttttgttaac taaacgacat tgaaatgtga
     9241 tactaaatcg ctatgcctcg tgccgtgtta acattctgtt caaaaacata accctgagct
     9301 gttgcatagt tcatataaac ggagtaatat tgcagtgtta ttggtgccta gtgaggctta
     9361 aaagggaacc cggtgtaaat ccggggctgt ccccgcagct gtaagctcat tcaaaggcct
     9421 tttactcccc tgccactgta atgtaattgc attatgggaa ggctgtaaaa ggttgagcaa
     9481 gccagaagac ctgccaattt gcttttttcg tagctttcgg gtgaaaagcg aaggatagaa
     9541 cacaaacttt ttttctgttc tattttcact aagtccgagc gctgcgaatg attaaaaacc
     9601 gttaatcatt tgtagcaatg tattttagag tcggtgtaat tttatacgct tttgccattt
     9661 cgggcattac agctgccaga gcacaaatgt gtcttgagga taccatacgt gtggaggagg
     9721 ttactattta ctcaacttac cgccagctcc atacaatcgg gacaaacaca ataaaaattg
     9781 acactttaag gttgacgaat tttgaaggca aaagccttac tgagttactt caatcaactg
     9841 gggtaaatat tcgttcatat ggagttggtg gacttgccac aatagctgtt aggggtgggg
     9901 gctcatcgca taccgctgtg gtttggaacg gaataaacct gcaaagccct ttgaatggag
     9961 gagtaaacct ttcgcaattc ccggttagcc tatttagctc ggttacagtt cagcatggtg
    10021 gcaatggaac agtttatggc agtggtgcag tatcggggat tgtgattctt gaaaatgggg
    10081 gcctactgct tcagccaaac ggagcaaggg ctggctttac ctatggaagc ggaaacacaa
    10141 ggggtgctaa tgcttcagta aagataggta attcaaaaag tgcattatcc ttgaagtata
    10201 gcggattaat tgccgataac gattttgaat tcattaatac ctacaagttt ggtaacccac
    10261 gtgagcgaat taccaatgca caggcagctc agcatgggat attagccgat gctgtattac
    10321 gtttcgataa taatgcactt tggaacattt cgggttggtt tacccataac gataagaatg
    10381 tccaaacttt gatgagcagc tctcagattt cccaggcgaa ccagctcgat aataattttg
    10441 ctgtatcgtc aaacctgaca gggaatttgc catttttttc tctcaaactc aggaacgcat
    10501 tcataaatgg cgataatcat tttaccgatc ccgcttccgg tattagcagc ttgaacacct
    10561 acaagcagct tgtaaacgag attgagctaa aaaaaacgat gcttaagcat caggagttgg
    10621 ttgtaggttt ggggtattct gttgatattg cttcatccca aagctacact gaaaatgcct
    10681 tacggaaccg catctcagct tacgcctcac ttgtgagcca tttgctgggt agtagaatta
    10741 agcttgtcct ctcgcttcgc gatgagttgg ttgatggttc gcttatacca ttggttgctt
    10801 caagtggact ggagtataaa gccacaaaaa gtttgatgct aagggcttca gcagcatcaa
    10861 gctaccgttt gccaacgctt aacgaccttt attggtcatc aaccacctat gcggtgggaa
    10921 accccaattt ggaaccggaa tatggatgga ataccgattt aggctttgat tacacaatta
    10981 ttcagaatgc cttaaatctc aaattctctg gttcctattt tattagcagg ttaaataggt
    11041 ggatagtttg gttacccgac gcacaggata acaataggtg gaaacctagt aatattagca
    11101 ctggcaaatc ggaagggttc gaaagtttca taattcttgg agtcaagttg atgccaattt
    11161 caatcagggc agatttaagt tacactttaa ccaattcaaa gatatacgac agcgggagct
    11221 acaatggcaa acccatgatt tacatcccca ggcataaagt tggagcatca atctcaatta
    11281 tttacgggca gtttaatttt gggtataatc attccttcac atcggagcga tataccgatg
    11341 agctaaacac gttgccctta tacaaccttg gtgatattta tatggggtac tcctgcaagg
    11401 catgggggag tgcgttcacg attggggtag gtgttaataa tgtatggaat gagcaatacc
    11461 agcttgtgcg taactatgcc atgcccctgc gtaattactt tattcggtta aatatcgatt
    11521 ttcaatccaa atcaactaat ataatcaact taaaaacttt taaaaatgaa cagaaaattt
    11581 tttaacctaa ccatttttgc cttcttggca tttgcatttt tttcgtgcga aaaggacgac
    11641 aaaaagcccg ggaagtttga aaacggagcc attgtgctca acgaagggtc attcatgcag
    11701 tcaaatgctt cggtgagctg ggttagttac cagtccgact cggtggtgaa taatgtgtat
    11761 gagaatgtta atgctcgtcc tttgggcgat gtgcttcagg gtggggtaaa ggccaatgga
    11821 aaaatctact tagtactgaa taactccggc aaggttgtta ttgttaatag ctctgatttt
    11881 attcaaacag gcgaagttag taatctcaat aacccacgtt atgccgtggt ggtcgatggc
    11941 aaattatttg tttcgcaatg gaatggctgg gggcaaaaag gtgctgtaat ggtatataac
    12001 gccaatggct ctgaaaaaat cgattcaatt ccagtgggta caggtgctga gggaatcata
    12061 tatgcagccg gtaaggtatg ggttgctaat agtggtggtt atggcgttga ttcaacggtt
    12121 acagtaataa atacttcaac ccttcaggtt gaaaaaacta taaatgttgg ctattgccct
    12181 aaagagttta ccgttgatgc aaatggtaag gtttgggtac tttgctcagg taccacaatt
    12241 tacgatgcaa actggaatgt tgctggtcat aaaccatcgt cgcttgcggc tataaataca
    12301 tcaacaatgt cagtggagaa gagtgtcaat ttattcaatg atatgcatcc atcgcatatc
    12361 gatatcagtg tggataagca aaccatttac tttggtggag gttttgggtt taatgggata
    12421 tacaatgtgc aaatagttgc tagttcaatt agtcaaaccc cattggtttc aggttatttt
    12481 tatgggttta atgtaaatcc tattaacggt gatatttata cccttgaggc gccaaatttt
    12541 accagtgcag gtagtttaaa ggtttacaat agcgatggga gcgggccaaa gaaaaccgct
    12601 tccgtgggta ttggtccaaa tggtgttctt tttaactaga tacctttatg aaatgtttaa
    12661 tgagctggag cgggggcaag gactccgctc tggctttata ccgatgtata aatgaccata
    12721 accataacat tgttggtctt gtaaccacca taaattcttc tacaaataga gtatcaatgc
    12781 atggtgttgc attggacgtg ctgaaatatc aggctgatgc aattggaata ccgcttcatg
    12841 ttattaccct ccctgagaaa tgtagtatgg atgaatacga aagtattatg ggagcattta
    12901 tgagcagaat gaaacaggat ggggttgaag gggtagtatt tggtgatatt ttccttgagg
    12961 atattaggaa ataccgtgaa agacagttgg agggaattgg tataactccc atttttccac
    13021 tctggggtct tgcgtcggcc gaagtggcaa gccaattcct tgataaccat tttaaatcaa
    13081 taattacctg tgttgatagc gacctgcttg acgagagttt tgtgggtcag ttctatactt
    13141 cagcgtacat tgaaaaactt cctggtggag ttgacccttg cggcgaaaat ggcgagtttc
    13201 acagctttgt tactgctggc ccaatctttc aaaaacccat tgaggtaaag gtaattagca
    13261 aaataagaaa ggaatacccc aatccccagg gcggaaatcc taaaactttt tggtttgccg
    13321 atattgcagc tagtaaataa cgaattggat aaagtagtta acccgtctat cgggcgatat
    13381 gctgaataac catgccgaaa tatcctcaaa ggtagtgagc tggtcgcctt cctggttcaa
    13441 cttatactta atgaatggta tgttatcttt tgtgaacaca aaaaccagtt tgttcgtttc
    13501 aggctctttt gcgctctttt caagggtata accatcaata agctcgctaa gaggaaaggt
    13561 gaccttttcg cccgctttaa atagcctact tttctcaaac tggttgggat aaataagcga
    13621 agcgttgttc tcgtaaaggt taaaaatatt caggtagcaa tcctgggttg gtgtaaccgt
    13681 gtaacgcata cgttcgccgc tttggtaacc ctgcttaatt ccatcgatgc ttacctggaa
    13741 tgcagggtct ttagctgttg tgtactgtat aacctcagcg tcaattatag cttcaacgta
    13801 aaaattctta aactcatcaa tccctttgtc tatttttact atgttatatt ttctgactgc
    13861 tccacgaatt tcggaaaaca tatcgctcat aaaaacttcg tcgaatttgt ttccaacctc
    13921 actcttgtaa agcatttcat acgattggat atgctcggtt actccggctt tcctaagagc
    13981 gtcaactttt gcttgggcaa ttgccttggc acgagcttca tcttcgctaa tacttcctgc
    14041 tattacctgt cggcccactg cattctcaac tttggttatt tcctgggcat tggtaaatag
    14101 accaattgga agcaggagaa ttaataagat ttttttcatg gcgatgtttt tcacttttct
    14161 cttcaaattt cgtgcaaaat attgatagtg gcaagcattt cccattaaaa aagcgaagtg
    14221 cccattaagg gcacttcaca ccagcaacga ttattccaaa ccttacctca aaactctctt
    14281 tccacatcct tatcgccacg tccggaaaga ttgacaatgg caagctggtt cttgtttttt
    14341 agcattttgg ctgccaacgc aaccgcatgg ctcgattcaa tggcagggat tattccctcc
    14401 agctgcgaga gcatttcaaa agctttaatg gcttcggtat cggtaactgc aaaatacttg
    14461 gctctactag tatccttaag gtaagcgtgc tgcgggctga tgccaggata gtctaaccca
    14521 gcagctacag agtgcgactc gcacgggttt ccgttgtcgt caaccaggca gtaggatagc
    14581 gttccctgga atactgttgg tttaccaaat gtaagagtag cggctgtatt accgggaatg
    14641 gcttcgccac cgccttctgc accatatatt tcaacctcta catcgttcaa aaaaccagag
    14701 aaaagaccaa tagcgttgga gccaccacca acgcaagcaa aaattgcgtt gggcaaacgg
    14761 ccctcctgca taagtatctg ttcacgagct tcgttcccaa taacgctttg aaagtaaccc
    14821 accattctgg ggtaggggtg aggcccaacc tgtgagccta aaaggtaaaa cgattcggga
    14881 tgttctatat agtacatcaa ggctgcgtca acagcatcct ttagagttgc tgttcccatt
    14941 gtagttgtta tcacttcggc tcctaaaagt ttcattcgtc gcacatttag cgcttgtcgc
    15001 tcggcatcct ttgctcccat aaatatcttg caaggaattc ccataagggc agcggctgtt
    15061 gcggttgcca caccgtgttg tccggcaccg gtttcagcaa ttatttccgt tgcacccatg
    15121 cgctttgcaa ccagtacttg tccaatggtg ttgttaattt tatgagcgcc ggtatggttt
    15181 aaatcttcgc gtttaagata aatggtcgat cctacttgtt ccgatagcct ttttgcccta
    15241 tataggggcg agggtcttcc tacatagtgc ttgagtagct gtacaaactc tttctgaaaa
    15301 ctagaatcct tagtaagcct atcaaactcg ttggccaagc gttccagtgg ctttgctaaa
    15361 acatcgggaa cgtaagctcc gccatattcg ccatagcggc cattatactt taacatgttg
    15421 cttaaaggct gaacaaattc atgctgtatg tttgaaatta ttgtttccat gatgataaaa
    15481 tttttagggt taaaaaatga cttgataaat gtacttgata gatgatttga acctttgtgg
    15541 cacgtagctc acagtaaaac aatggctatg cggggaggcc ccgccaccac catggagcga
    15601 aaacagcaat ttgttgtatt tgtcggtaaa actggatcat catctactta ttaaattaaa
    15661 aaagccgtag ggggtaacct acggctgtat attgcaaagc aatggcctaa ctaatccccc
    15721 tgtgttcgga ttaatttgtg ccaccaccaa atattgctta atcgtttcat cgttgtctat
    15781 tcttttgcaa tcttaaaaag atttttttta aaatccaaat tgggctatgg cattttgttc
    15841 catattttat tgtgataatc aaggtaaatg ggcaatgcag ccgaaccgta ccagttaaaa
    15901 atttcgggtt ttagaattcc tgaacttact gctggatcgg tattaaccat ttcctccact
    15961 ttattaatgt cagtttcgta aaaaatgaat aatcccctgt aaccatatgg gtttgctcca
    16021 aacggcccag caacaaagag tttaccttcg ttttctaaac ggattatgtt gttcatatgg
    16081 cctctaaaaa tactatctgt ttcacttttt gaaaggtttt gcttctcgcc cgattttagt
    16141 agaacgaatg tgtataaccg cataccgtat tcatcggcat ttagcttttt tgcaagctct
    16201 ggatcgtaat taatgttttg tgcaagcaaa aggtttgtgc ctgcaacaaa tagtgttaat
    16261 gcaatcaata tctttttcat tgttcatata ataatgttca acacaaagcg ttaaattaat
    16321 atactgtcat atcaaataaa acaaaagtat gaaaagaatt acattttgca taggattaag
    16381 cgtaatgtca ttactaggct atggtcaaat tggcaatgaa attttaacaa gtattgcatt
    16441 aggtaagtat tccgttgctc ttgaacaaat taatgagttg cctccatcac ctgagttaat
    16501 ccaattcaag gcaatagctt ttggggggtt aaacatgtgg gatagtgcag tggtttacta
    16561 ttcaaaactt acaaatatcg acactcagct tgcgaagaaa ggattagcca ggtgttacga
    16621 gagtgttggt aactcatcca aagccattga cctttacaaa caaatactag cagatgattc
    16681 cttgaatgct agcataataa ccaggtatgc aaacctgctt aggagtagtt cattgcataa
    16741 ccaggctttg gaacagttcc aaaagctaat tgaattggat tcgacatgtg ctaaatactg
    16801 ggaaatgttg ggtgaccagt acaaggcaat gaacatgact gttgaaagta ctaccgcata
    16861 caataaagca tatctgttaa actctaataa tctttttgtt gcttcaaaat acttcgctca
    16921 actgattaaa agtaatgtgc cattacagta agtgtacgag aatattcttg atgcctatca
    16981 ggttgactca acttacatgc caattcttat gcttaagggg aaggttgaat cggatatgaa
    17041 aaagtatagt caggccgaaa taactttcac aaggattgct tcgcttggcg actcatcatt
    17101 ctttacctta aaaaatcttg ctattgccaa gcatagcaac ggttcatatc tggaagcaga
    17161 aaatctcttt ataaaggctt atgagatgga tagtaccgac tttcttctaa attttgttta
    17221 cgcaaaaaca ttgcagaatg ttggtgagcg caatctggca ctggatatta taagtaatac
    17281 cattaagctt ctttacccac ccgatgagct ggtagcatca ttttatgagc tagcaggaga
    17341 tatttataac tccggcaaca aaattgattt ggcagttgaa aactatcaaa tggctcttga
    17401 gaaagataaa aaaaatgagc ttaagtacct gaataagatt atttggtgca agatttatga
    17461 taagaaattc agagatgctg aatacctggt acataaatac gatagcattg cccaggaaaa
    17521 atatgctgat gacagcatcc agctaaaaaa cgaacagaga aaaactgggt tttataaaga
    17581 tttacttaaa aaggagtttt tcttcatgga tgatttaaaa aacgggaaat tccttgttta
    17641 ataaacaaat ccctttcaaa aggtgttcct atagtatggt gcacctttct tatataattc
    17701 tatacgactc atcgtgtaat ctatgccaaa aaagcgtacg ctttataaag cgttacgata
    17761 aaaggcaact atttagtttc atgccgtttg gctcaaatga tgcaggtctg attgtcagtc
    17821 gttatggggt tagtaagcag tataaagaat caatggtttt cgttctaaag ggaaaagttt
    17881 acacaaaatc aactgcagca ttactgattt gttggcacct tagtgggttt tggccaatac
    17941 tgtcaatatt cttgctgata cctactccaa taagggattt tttctatgac ataatagctc
    18001 gtaaccgtta ccgctggttc ggtaaatgtg aaagctgcgt tactgaatag tcaatatcat
    18061 ttttgaatta aatcaatggg tatgagcctt aacggctcaa tgctttcagg tgtaacattg
    18121 acctgaacgg caaatagttt tactcctgca tcaaacgcct ttattagctc tttagcatat
    18181 gcagagtcaa tactccaagc gggagcaaat atatccacat cggtgcgttg aataatgtat
    18241 atcataacta catggtaacc ttcagcttta gctttaatca gagtgcttag gtgtttttgc
    18301 ccacgtgttg tcacagcatc ggggaattgg gcaaacctcc cttccttcaa cgttacgttt
    18361 ttcacctcaa agaaccatct ttgattttcg gattcaccgt aaatgtcgaa acggctatcg
    18421 tcgtatttga cttctcttat taaagtttta aatgtaggta gctcatcaat ccaatggttt
    18481 ttcagaatat cataggcaat cctgttggga ttcgaggtgt ttattcctac ccagccatta
    18541 ttaatcctaa tcatttccca ggtatagggt aactttcggt ttggattaga gcttgggctt
    18601 atgaatacct cagcgttttc ttccaggcag cttttcattg agcccgaatt ggtgcaatgc
    18661 gctgtaacta cattcccatt gtcgagtaga atatcggcca agaatcgttt atagcgttta
    18721 acaagcctgc cgtggattag gggttggtca aatttcatat tatgtacaga tgttgcgttt
    18781 aaacaggttt tcaccttgaa tgttcaaaaa agcgattgtt gctcagggta tgagtagggt
    18841 tccaaaacct cgggaacatt ccttttagcc ttaggattcg aaataattgg tgatactgta
    18901 tacgccttta agtattcctg ttctacgggt ttaattatat cagagtaaag ggaggttttt
    18961 aaatccttat ccaaccattt ttctgcattt tcgggcttaa gtatggctgg cattcgtttc
    19021 ttagtattat gaattttttc gagtaatggg tttgccggta tagtgataat gctaaaggtg
    19081 tcaaccattt cacctgtttc agtattagtc catgtgctgt aaattccagc taaatgcatt
    19141 atttcattat tatttaagcc aataaaccat ggtatttttt taccatgtat atgctgccat
    19201 tcaaaaaaac cggtagcagg tactatgcat ctgttgcttt ttgctgagta tcggaacgat
    19261 ggtttttcaa gaatgctttc gaatcgtgca ttcagcgttt tctctttaat ggattgagct
    19321 tcgatagggt tttttaccca gttgggtatt aggccccagc tcattggtgt aaaatattct
    19381 ggattacccg atgcaaccac aggaataaca ggaaaatcaa aagctgatac aaagtagaag
    19441 ggttcaaagc gaaaatcctc tctgaaccga gcgttcatga ctttctcaag tctatgcttt
    19501 tcaactcgta cgcttaacgt gaaacacatt aggtgttgga tgatttaaca tttctttgtt
    19561 tacaagacta acctgcttaa aatcttatta acattcaaaa cctttgatat ctagttaagt
    19621 aagaaatcat acaaaccaaa ataagaggag gtatttacta ataaccctcc ccttaatcag
    19681 attcgtaaag gcaatttata ttcctcaaaa cagaacagta ttagtccttt aaacagactt
//
LOCUS       DAOSAL010000048        19397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_1977_length_19397_cov_51.304053, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000048 DAOSAL010000000
VERSION     DAOSAL010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 19397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19397
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1977_length_19397_cov_51.304053"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PK049_07645"
     CDS             <1..189
                     /locus_tag="PK049_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61469.1"
                     /translation="DPMGAPWVELPRDTIRHACGVTHVVSLTKPTATFHPRPIAGSGF
                     YATACFIRSIPSQGKGLG"
     gene            664..1137
                     /locus_tag="PK049_07650"
     CDS             664..1137
                     /locus_tag="PK049_07650"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOK61470.1"
                     /translation="MENLKYAGFWWRFLAYILDAIIIGVIQWIIVIPVLALIGFSAAS
                     AASNGITETEAIGMVGAIVGTAMITYLALIVLMWLYFAIMESSKFQGTVGKIAIGLVV
                     TDMEGNRISFARATGRYFGKILSSLIFMIGYILAGFTQKKQALHDMLAGTLVWKK"
     gene            complement(1202..2446)
                     /locus_tag="PK049_07655"
     CDS             complement(1202..2446)
                     /locus_tag="PK049_07655"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61471.1"
                     /translation="MIKYQITKSDTKIYGEVTIPPTRPMSNKFLVIKVYKDANLPTGQ
                     GSEIGNEKIINQSIFKEGLKSKSSRTARAIRHIRAFINYFGGEWILSSSNILKDSTTG
                     KIVALLQKYGLKVQFEERIGNPPFRLIGKNLKGKILRVEGSINSKMIEARLLIDPSLN
                     SEEIIELKNFIISQGYSTMTLRALQFMGVNIDWREDEVLIEHQIFDGSELSIEADWSL
                     ASYWYEAVALADKGEIAIKGLKPESFQCDTAVKDLFTRLGVTTIIAENAIILKRKGRV
                     TKQFEHNFKQHPDLAPAAMFTCLALGIPFTISGIDYFHTKDPDRLNAIKAEFQKLGAT
                     INVQLENDVEVLKFDGKSKLKNLKVLECDSQNDHRVAMSLAPYAILGKRVVMNNARIT
                     SKSYPTFWDEIKKLGFEVTVLQ"
     gene            complement(2496..3299)
                     /gene="cdaA"
                     /locus_tag="PK049_07660"
     CDS             complement(2496..3299)
                     /gene="cdaA"
                     /locus_tag="PK049_07660"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOK61472.1"
                     /translation="MELLPIPLFIHVRVLDIVDIVIVAFLLYQIYLLIRGTAAIKIFS
                     GIVLLYFIWLVVKALNMELLGNLLGQVIGVGVIALIIVFQQEIRKFLLLLGSRYTTKG
                     KSALESFLLGTDQNEVSQTNLDAIVKACKNLSETKTGALIAITRNSELEVYSQTGDII
                     NAELSSRLLENIFFKNSPLHDGAVIIKGNKIHAARCVLPISENLNLPPKFGMRHKSAL
                     GLTEVTDALVIVVSEETGKISLADNGSIDYGISLTDLKEKLEQKLQAKR"
     gene            complement(3304..4185)
                     /gene="folP"
                     /locus_tag="PK049_07665"
     CDS             complement(3304..4185)
                     /gene="folP"
                     /locus_tag="PK049_07665"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOK61473.1"
                     /translation="MEEKRSVFAPRKTLQFNGRLITIEKPLVMGIINITNDSFFSGSR
                     YRFSHSIARRAKQIVEEGGQIIDIGACSTRPGAKPVSERDEIKRLHKALAAIRRSYPE
                     AIISVDTFRASVAEWAVKEFRVDIINDISAGQMDSKMFETIAELNVPYILMHMQGTPE
                     NMQQNPQYENVIRDLILFFNDKIEKLKQLNVKDIIIDPGFGFGKTIEHNYTILKNLET
                     FKLLELPILVGISRKSMIYKPLNTKPEQALNGTTVLNTLAILKGASILRVHDVKQAAE
                     TIRLLELLNAQPEFQFI"
     gene            4274..4966
                     /gene="tmk"
                     /locus_tag="PK049_07670"
     CDS             4274..4966
                     /gene="tmk"
                     /locus_tag="PK049_07670"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOK61474.1"
                     /translation="MALIVIEGLDGAGKSTQIDLLTQHLKTKSIPCHFLHFPRVDAPF
                     FGDLIARFLRGDLGTIDRVDPYVVALLYASDRMDAAPMIRQWLNEKHVVLLDRYVYSN
                     VAFQCAKLNNPHKINLLREWILKLEFDYYAIPKPNLNIFLNVPFEFTRQRLTEQRIGE
                     SRQYLQGKDDIHEKDLSFQEKVRNIYLQQQQFDPNFVVLDCTDKEGHMLKPEAISSMI
                     LNEIAKRNLLNL"
     gene            4978..6198
                     /locus_tag="PK049_07675"
     CDS             4978..6198
                     /locus_tag="PK049_07675"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOK61475.1"
                     /translation="MKRRILVVARYLLAIVFIFSGFVKGIDPWGTAYKLHDYFNAFHF
                     HFLDNLALPLSVLLCAAELYIGLLLLSGVKQKIAAWGAFLFMVVFTPLTLLLAITNPI
                     SDCGCFGDAIKLSNWGTFFKNLVFFAAAAFLFTNRREELSRQNPIKQNLLSLLLLAIA
                     ILPSVHGIRHQPIIDFRPFHIGANLLQYTSIPEGAPHDVYRTILYYQKDGVVKEFDET
                     NYPWNDSTWTFVDSKSYLVEKGYTPPIANFTLYTEDGHNYTDSILYSKGDLILVVIPN
                     IKYLSEPAIARFKTIQKQARDKGVKILGLTSAAGTDLANLENQHGLFFTWLQADEVML
                     KTIVRSYPGMLYLYNGTIIGKWHWRDIPETLFKTEQPLSFLVTASQKKWEYAIASILS
                     LSLILILILFNRNK"
     gene            6214..8400
                     /locus_tag="PK049_07680"
     CDS             6214..8400
                     /locus_tag="PK049_07680"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOK61476.1"
                     /translation="MRAIKNLTSWLLLFLISSNVLADEGMWMLNLIGKNISAMQTLGL
                     KLTAEDIYSVNKSSLKDAIVQFDDGSCSAEMVSPNGLFFTNHHCGVDAVQELSTVSNN
                     LLENGYWAKSYAEELPAKGKTALILVDITDVTSDILTGISPSLSNREYFEAINQKMQY
                     LQDSVEAKSGYHVVVRPFFNYNEFYMFRYERYLDVRLVGVPPASIGNFGGDVDNWHWP
                     RHTGDFCIFRIYTSPDGKPAQYSPKNIPLKSKNYLKINLQGVQEGDFAMIIGFPGRTH
                     RYASSYEAMFNREVVANFKRTVWGEMINAIKKAQQTDPAIKVNYTDKHDYLINFYQKD
                     VWQAESMYKYSVVERLAAREDSLKAWANSNPFLYSRYVTSIPVLENYYQTVTQNRWEE
                     LEGAISALSFYPVDVYKNVTACNDFVSAIIMQGKPYSRLSFKRDYIRQEAKLLEKRLP
                     KIFEKYHIYPDVQLYSIAFGALLNNSGEFNNLGMLNAIKKVEDISTSYPYYIMAFFEK
                     SYFTSPENLKKLIKRPYLDSLVKDPLFILYHNYNMLWDSIYNSMYEAKQNYNRAMQLY
                     TKGIMEMNKGKLLYPDANSTPRLTYGKVKGYRPADGMAYKPFTTLDGVMEKEDPNSDV
                     FKVPEKLRKLWERKDYGPYARNGVMPVCFLTDNDITGGNSGSGVLDANGNLIGIAFDG
                     NAEAMACDFMYEPETQRTIVVDIRYVLFIIDKFGESKHIMNELTIL"
     gene            complement(8451..8846)
                     /locus_tag="PK049_07685"
     CDS             complement(8451..8846)
                     /locus_tag="PK049_07685"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOK61477.1"
                     /translation="MEFEVTFQGKKQVVAHIGEHDILTDQPVYGGGDNQGPAPFTLFL
                     ASIGTCAGIYVKSFCDQRGIPTDNIKIVQKHQFNPLTHMIERLELEINLPADFPEKYK
                     EAVIKAADQCAVKKHLHNPPQMSVTTKSI"
     gene            complement(9091..12504)
                     /locus_tag="PK049_07690"
     CDS             complement(9091..12504)
                     /locus_tag="PK049_07690"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HOK61478.1"
                     /translation="MRKNLLGLVSICLMTCLLWVNQTQAVPAYPGLVQYKQANGKTLA
                     IFLKGDEKVKWATTEDGYTIMLNSNGVYEYAIQDMKGDLQLSGIEVNNIKERTAAEKE
                     LLSKVSKGLYYSPSQLSMMKSIWDIKSAEAQKAFPTTGNRKLICILMGFKDKAFTKTQ
                     SDFNNLFNQVGYSTGGATGSVKDYYLENSWGQFNLTVDVFGPYTASQNMSYYGANDAY
                     GNDKNPRALVTEAVNAADASANFANYDNDGDGTVDGVYVIYAGYGEEAGASADAIWAH
                     AWSITPVTKDGKTISKYSCSAELRGNSGTTITSIGVICHEFGHVLGAPDYYDTDYSTG
                     GQFEGTGKWDMMASGSWNNNGVTPAHHNAYTKVKVYGWATATVLSSATNVTVKPVKDN
                     KSFFQINSTTSGEYWIMENRQQVGFDAYVPGHGLIIYHVHKDVASASSSNNINATYPQ
                     KMYPVCASATTNPGTTSSTYGSINSTGCPFPGSSNKTSFTDATTPNMKSWAGANTGKP
                     MTSIAENATTKDITFAFMGGSGGGTAPTATTVAASSITTSSATLNGTVNANNATTTVT
                     FEWGTTTSLGNSINATPNSVTGTSNTNVTANLTGLSANTKYYYRVKAVSANGTTYGST
                     MNFTTAVNPTSVTLPYSQTFSSSSMPTGWTTQNTGSGITERWSVSNTNKAGGSAYEMK
                     CTYQNVNPGTTRLITPAINTSGVSQVTLTFRHMLDAYSTGVTLRVQTSNDKTNWTNTS
                     WSVATSSTNINATSVTVNITTNLNSATTYIAFVAEGNLYNIDYWYIDNVSVAAGSGAT
                     TPTVTTGSVSNITSSTATVAGNVTADGGATVTERGICYSTSQNPTTANSKVASGSGTG
                     SFSANLSGLAANTTYYARAYAINAQGTSYGSQVSFTTTTSTVTYCASKGSNSSYEWID
                     LVQFAGINRTSGNDGGYKDMTSLQATVARGTTYTIYFSAGFKSSSYTEYWAVWIDYNK
                     NGTFDTNEKVVSGSSSSSGTLSASITIPTTATLGVTRMRVSMKYNAAPTACETFSYGE
                     VEDYSVNITSGTSAPSTDNPFAEELGNEKVDIFTIYPNPASEKINVVLNGIEGEVSAR
                     IYDMRGAVVKFQMLTDRNNTIEINDLAPGVYMISIDDEKQPITKQFIKK"
     gene            12767..13468
                     /locus_tag="PK049_07695"
     CDS             12767..13468
                     /locus_tag="PK049_07695"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOK61479.1"
                     /translation="MDLKAIIVDDEPLAIAVIDGYLSRIPGVEVVATFNDGLPAFEYL
                     QENPVDIVFLDVEMPKLTGIELVRCLYNPPVVIITSANRDYAIEGFELNVADYILKPV
                     TFERLLKAFSKAKELLSKRAENTSPKDSEYLIFKESRKSVRVKQDEILYFESIKDYVK
                     VVTPNRTVITKQSISSLEELLDKEKFIRVHRSFIVALNRVDTFSTTSLEIGDIEIPIG
                     RLYKEDVIKILETKK"
     gene            13475..>13890
                     /locus_tag="PK049_07700"
     CDS             13475..>13890
                     /locus_tag="PK049_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HOK61480.1"
                     /translation="MLRRLKYPLLIIVLVQLTFANAQERGLLLSHYYSANTYQAATQN
                     WGIVQDKRGVIYFANGAGVLEFDGKNWQFIEVGKGASVRSIAIDSNDRIYVGGYGEFG
                     LLSPDKTGLLRYVSLSSHVDSAYKEFNEVWDTRVIA"
     assembly_gap    13891..13990
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13991..16117
                     /locus_tag="PK049_07705"
     CDS             <13991..16117
                     /locus_tag="PK049_07705"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOK61481.1"
                     /translation="TFKPDRFNRLEGRYEQAWQFLYFQEPSDTTLLKPLWYGSNQKTH
                     LLVAARNGVFEINKRSASKITRLELPYVLHQGRINRSYLFVGHENGVSRLKYKNGRWL
                     NEALLCKTNRKILSIGEDSNGNVWFSENLGGVGCIVNAQLEKSEDEVFRYDTSNGLPD
                     AAYVRIFDIYDTILFIANSKFYSFNQDAKRFEPFDIKSLNILEWLPSNNDTLMWRRIS
                     NQLLTDQYVTHFADSISWVSTDKGIFQVRPAKGYNYFNLPAPIIYRAINMDSVLFNGN
                     NYSLVEGDTVFGAYLYKKIADPIIDLGIVLPYKRNSVKFSYSWPYFESDQPCQFSYQL
                     VGNDEGWSEWSTDYRKEYTNLREGKYVFKVKARSVFGVESPVLEFRFEVKTPWYRTIY
                     AYIGYALLVFLLIYSIFKIWHYKLILERDKLERLVKERTQEILLQKEELQVQAEHLRE
                     TYEWISEKNAMLEKQKSEIEQQKQKLEEINATKNKFFRIIAHDLRNPISTLVNSTAFL
                     LTDIETLSSEKTKKFMGELNRLALTTYGLLENLLDWSSNEMGDIQNKPQRVSLALLVN
                     QNIELVQNRLTDKNIKLTVDIPASIELYVDENMLNTVLRNLISNAVKFTRPGGEISID
                     ASEVNGKCVIRVKDNGIGIPDQNIEKLFRIDKSIVTPGTQNEKGSGLGLLLCKEFVDK
                     MGGTISVNSRQGFGTTFSIEFPVDIL"
     gene            16198..17403
                     /locus_tag="PK049_07710"
     CDS             16198..17403
                     /locus_tag="PK049_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOK61482.1"
                     /translation="MKRIKYFFSLLLFLIMAIGVVFAQDKKDELKEPYAFTDVKVIPT
                     TSVKNQNRSSTCWSFSGMAFLESELLRMGKPAVDLSPMFVVRNIYAMKADRYVRFNGT
                     NNFGPGGSFFDVLEAIKRFGIVPMEVYQGLSYGEESHVHGEVDAVTKAFVDAIVKNPN
                     RKLSTAWKKAFDGILDAYFGSLPQNFTYNGKTYTPELFAKQLGINPDDYVVITSFSHH
                     PFYQKFVLELPDNWIHGEAYNVPLSDFDRIIESAIDNGYPVAWASDVSDKGFNWSKGV
                     AIVPDFDDKENAGSDKERWTQLSNREKEKTLYSFEKPVKEMVITQEIRQLAFDNYQTT
                     DDHGMVLVGKATDQLGNAFYKVKNSWGADGKYKGYFYASRAFVLYKSTNIMVHKKAVP
                     VDLAKKLGI"
     gene            17513..17884
                     /locus_tag="PK049_07715"
     CDS             17513..17884
                     /locus_tag="PK049_07715"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOK61483.1"
                     /translation="MTNLYIKGTTKTPEIDFKPGILQISGRSIAEDAVAFYQPVIKWI
                     EDYLKKPEPLTRLNLKMEYINSGSNRFIFTIMRMLDEAYSQGHNVVINWYFEEDDDTI
                     KNLGRDFQALVKAPFKMVEII"
     gene            complement(17881..18579)
                     /locus_tag="PK049_07720"
     CDS             complement(17881..18579)
                     /locus_tag="PK049_07720"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HOK61484.1"
                     /translation="MKMLWLTLQADSFQLFGIDIINTNDFYTLLLRFILNTVILWIAV
                     RYLYYPRTYRKDYVFTYLMLGVVVFFMCFLLANVKLQLGFALGLFAVFGIIRYRTNPI
                     PIKEMTYLFFIIAISLINAISSKKVSYAELLFSNFALLGVALILEKGLFIKHLSQKVI
                     VYEKINLITPDKRAELIEDLKKRTGLEIKSVEIGKVDFVRDIAWLVVHFPAENFNDTN
                     TPQIDAREVVDDDD"
     gene            complement(18548..19321)
                     /locus_tag="PK049_07725"
     CDS             complement(18548..19321)
                     /locus_tag="PK049_07725"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTC domain-containing protein"
                     /protein_id="HOK61485.1"
                     /translation="MKENDIKQLDFNNLNLGDVGKAQFMRRIDSKYLVQAEWAFEILS
                     FLKQHYFIVENKAQLMPEYFSEYFDSSGFTMYLDHHNRRAKRYKVRLRHYRASGDVFL
                     EVKTRMPSGETLKKRTEITNNSIDNPTVEAFVKKNIPFRFNELTRTLYTTFNRITLVS
                     FDFNERVTMDFNIQLKNPTHAVNLSDICVIESKRDKYNSSSFLINHLKQHGYRQTGFS
                     KYSIGCALLYNNLKSNNFKPIITHINKYKNENALVNTTS"
BASE COUNT         5506 a         3769 c         4085 g         5937 t
ORIGIN      
        1 gacccgatgg gtgccccgtg ggtggaactg ccacgcgata ctattcgcca cgcctgtggc
       61 gtgactcatg tggtatcgct tactaagccc accgccacgt tccacccccg acccattgcc
      121 gggtcaggct tttatgccac tgcctgcttc atccgttcca ttccctctca gggaaaaggg
      181 ttggggtgag gtcgtatccc acgaataacg aacaacttat acgaatagtt tcatttgtca
      241 tcctgagtgc agcgaaggat cttttgaata gtgtgcagtg tacagtgtgc agtttaaagg
      301 gaaaatggag agtagtgttt ggttttaagt gtttcatgaa atgcattttt tatcactaac
      361 aactatcaac catcaactgt caactccgct tgtcatcttg agcgcagcga aggatctcta
      421 ttcatccatc gttgagatgt tttagcgaag tctatcccta taaatccgga ctcagcacga
      481 cagcgtcatg tttcgcgcaa gcgaaacatg acaaataacc ttaactaaac aacattggtt
      541 ccagaaatag caatttggca agaatttgta gataattagg tttgattgtt ttgaaactac
      601 atggaattta ttatattgct gttgatttaa ataaccataa actaaactta aaccttacga
      661 attatggaaa acttaaagta tgcaggtttt tggtggcgct ttctggcgta cattcttgat
      721 gcaataatta ttggtgtaat tcagtggatt attgttatac ctgttcttgc attaattgga
      781 ttctcagcgg cctcggctgc gtcaaatggg attactgaga ctgaagccat tggtatggtt
      841 ggcgcaattg ttggtacagc catgattacc tatttggcgt tgattgtact aatgtggtta
      901 tactttgcca taatggagtc gtcaaagttc caaggaacgg ttggtaagat tgctattggc
      961 cttgttgtaa ctgatatgga aggaaatcgt atatcgtttg cccgagctac cggacgctat
     1021 ttcggaaaaa ttctttccag cttgattttc atgatagggt atattttagc aggttttacc
     1081 caaaagaaac aagcacttca cgatatgctt gctggtacat tagtttggaa aaagtagatt
     1141 ataccaaaaa tagaaaaggg tggcagagat gtcacccttt tgctgtttaa gaattttctt
     1201 tttactgcaa aactgttacc tcaaaaccta actttttaat ctcatcccaa aaagttgggt
     1261 atgatttact ggttatacga gcattgttca taaccactct cttgccaaga attgcgtagg
     1321 gggctagcga catggctaca cggtgatcgt tttgtgaatc gcactctaga actttcaagt
     1381 tttttagttt ggattttcca tcaaacttca gaacttcaac atcattttcc agttgaacat
     1441 taattgttgc accaagtttc tggaattcgg ctttaatggc attgagcctg tcggggtctt
     1501 tggtatggaa gtaatcaatt ccactaattg taaaaggaat gcctagtgca aggcaggtaa
     1561 acatcgcggc aggggctaaa tcggggtgtt gcttaaaatt atgttcaaat tgctttgtta
     1621 cccttccttt gcgtttaaga attattgcat tctctgcaat aattgtggtt acacctaacc
     1681 gtgtgaataa gtcttttaca gcagtatcgc actgaaaact ttcgggtttc agacctttga
     1741 tagcaatctc ccctttatcg gcaagggcta ctgcctcgta ccagtacgat gctaacgacc
     1801 aatcagcttc aatggaaagt tcactaccat caaagatctg atgttcaata agcacctcat
     1861 cctcacgcca atctatattt acgcccatga actgaagcgc tcgaagggtc atggtggagt
     1921 aaccctggga aatgataaag ttttttagtt caataatttc ttcggaattt aaacttgggt
     1981 cgataagcaa tcgtgcttca atcatcttgc tatttattga tccttcaacc cgaagtattt
     2041 tccccttcaa attctttcca ataagtctga aaggcggatt accaattcgt tcttcgaact
     2101 gaacctttaa cccatacttt tggagtagcg ctacaatttt accggtggtg ctgtccttta
     2161 agatgtttga cgatgaaagt atccactcgc caccaaagta gttgataaag gccctgatat
     2221 gcctaattgc tcgggctgta cggctactct tgctctttag tccttcctta aagatgcttt
     2281 ggttaataat tttttcgttg ccaatttcag aaccctgtcc tgtgggaaga tttgcatctt
     2341 tgtaaacctt aattactaaa aacttatttg acattggtcg ggtggggggt attgtaacct
     2401 caccataaat ctttgtgtcc gatttggtga tttggtactt aatcattgct gataagattt
     2461 aggtgttgaa caaatgtaac ataaacttta ttatcctatc gtttagcttg taacttttgt
     2521 tctagtttct cttttaggtc agtaaggcta atgccataat cgatactgcc attgtcggct
     2581 aagcttattt tgcctgtttc ttctgaaaca acaattacca aggcatccgt aacctctgtc
     2641 aatcctaagg ccgatttatg cctcattccg aactttgggg gtaaattaag gttctccgat
     2701 attggcaaaa cacatcgggc agcatgaatc ttattgcctt tgattattac cgccccatcg
     2761 tgtaatgggc tatttttgaa gaaaatattt tcaagcagcc ggcttgagag ttctgcgtta
     2821 ataatgtcgc ccgtttgaga gtatacctca agctctgagt ttcgggtaat ggcaattagt
     2881 gcccctgttt tggtttccga taggtttttg caagccttta ctattgcatc gaggtttgtt
     2941 tggctaacct cattctgatc cgtacctagc aagaaagact ccaaggcact tttcccttta
     3001 gttgtgtacc gcgacccaag cagcaacaaa aactttctta tctcctgctg gaaaacaata
     3061 atgagggcaa taacaccaac cccaattacc tggccaagca aattgccaag taactccatg
     3121 ttcaatgctt ttacaactag ccaaatgaag tacaggagca caatccctga aaaaattttt
     3181 atggcggcag tccctctaat taaaaggtat atttggtaaa gaagaaatgc tacaattaca
     3241 atgtcgacaa tgtcgagcac tcttacatgt ataaaaagcg gtatcggtaa taattccatg
     3301 gggctaaatg aattgaaatt caggttgagc attaagtagt tcaagcaatc ttatggtttc
     3361 agcagcttgt ttaacatcat gaaccctaag tatgctggca cccttaagaa ttgctaacgt
     3421 gttcagaacc gttgtcccat ttaatgcttg ttcgggttta gtgtttagtg gtttgtatat
     3481 catcgacttc cttgaaatgc ctacaagtat cggaagttct agtagtttaa atgtctcaag
     3541 gttttttagg atagtatagt tatgctcaat ggttttgcca aaaccaaagc cggggtcaat
     3601 aattatatcc ttaacgttaa gctgtttcag cttctcaatt ttatcgttaa agaatagaat
     3661 gagatctctg ataacatttt cgtattgagg gttttgctgc atattctcgg gtgtaccttg
     3721 catatgcatg agtatgtagg gtacatttag ttcagcaata gtttcaaaca tcttgctgtc
     3781 catctgtcct gccgatatat cgttgattat atcaacgcga aattccttta cagcccattc
     3841 tgcaaccgaa gcacggaagg tgtctactga gattatggct tcaggataac tccttcgaat
     3901 agcagccaac gctttgtgga ggcgttttat ttcatcccgc tcacttaccg gtttcgcacc
     3961 cggtcgggtt gagcaagcac caatgtcaat tatctggcca ccttcctcaa ctatttgttt
     4021 tgccctgcgg gctatgctgt gcgaaaacct gtaccggcta ccgctaaaaa atgaatcgtt
     4081 tgttatgttt attattccca taaccaaggg tttctcaatg gttatgagtc tgccgttgaa
     4141 ctgcaacgtt tttcttggcg cgaaaactga acgtttttct tccatttttt cctatcttgc
     4201 aaaatgtttc aaatgctaaa attacgaaac attgtgttaa gtttacagat gttgtttttt
     4261 aattttttcg cagatggctt taattgttat tgaagggctc gatggtgcag gcaaatcaac
     4321 ccaaattgac ttacttacac aacaccttaa aacaaagtcc ataccttgcc atttccttca
     4381 ctttcctaga gttgatgccc cattttttgg cgatcttatt gctaggtttc tacgtggcga
     4441 tttgggaact atcgacaggg ttgatcctta tgtggtagcc cttttgtatg cttcggatag
     4501 gatggatgct gctccaatga ttcgccaatg gcttaatgaa aagcatgtgg ttctgctcga
     4561 taggtatgta tactccaacg ttgcctttca gtgtgcaaag cttaacaatc cccataaaat
     4621 taacctgtta agggaatgga ttttaaagct ggaatttgat tactatgcaa tacctaaacc
     4681 caacttaaat atctttttaa atgtaccttt tgaatttacc cgccaacgtc taactgagca
     4741 aagaattggt gagtcaaggc aatacttgca gggtaaggac gatattcacg aaaaggacct
     4801 atcatttcag gagaaagttc ggaatatcta cctacagcag caacagttcg acccaaattt
     4861 tgttgtatta gattgtactg acaaagaggg gcatatgctc aagcctgaag caatttcgtc
     4921 aatgatactg aatgaaattg caaaacggaa tttgttgaat ttataaagaa aatggcaatg
     4981 aaaaggagaa ttttagtagt tgcaaggtat ctgttggcaa tagtttttat tttctcaggg
     5041 tttgtaaagg gaattgaccc gtggggtact gcttacaagt tacatgatta ctttaatgcg
     5101 tttcatttcc atttcctgga caatttagca ctccctcttt ctgttttgct ttgcgccgct
     5161 gagctgtaca ttggcttgct attactatca ggggtaaaac agaagattgc tgcatggggg
     5221 gcattcctat tcatggtagt atttacaccc ctgacccttt tgctggctat caccaatccc
     5281 atctctgatt gtggttgttt tggtgatgcc ataaagctat cgaattgggg aacctttttt
     5341 aaaaatttag ttttttttgc tgcggctgca ttccttttta ccaaccgtag ggaggaacta
     5401 tcaaggcaaa atcctatcaa acagaatttg ctttcattac tgcttcttgc cattgcaatt
     5461 ttaccctctg ttcatggcat acgccatcag cccattatcg attttagacc tttccacatt
     5521 ggtgctaatt tattacagta cacctcaatc cctgaagggg cacctcacga tgtttacaga
     5581 acaattcttt actaccagaa ggatggcgtg gtaaaggagt ttgatgaaac caactacccg
     5641 tggaatgatt ctacatggac ttttgttgat tcaaagtcat atttagtaga aaaaggctac
     5701 acccctccta tagctaactt cacgttgtac actgaagatg gacataacta taccgatagc
     5761 attctttact ctaaaggtga tttgatttta gttgtaattc caaatattaa atacttatcg
     5821 gaacccgcaa tagctaggtt taaaactatt cagaaacagg ctcgcgataa aggcgttaaa
     5881 atcctcgggt taacatcggc tgctggaaca gatttggcaa acctggaaaa ccaacatggt
     5941 ttattcttta catggcttca agccgatgag gttatgctaa aaaccatagt ccgatcgtac
     6001 cctggtatgc tttatttata caatggtaca ataattggca aatggcactg gcgcgacatc
     6061 cctgaaaccc tttttaaaac cgagcaacct ttatcatttt tagtaactgc ttcacaaaaa
     6121 aagtgggagt atgctattgc ttcaatatta tcgctatctt taatattaat tctgatcttg
     6181 tttaatcgaa acaaataaat caaaatttcc aatatgagag caataaagaa cctgaccagt
     6241 tggcttttac tttttcttat ttcttccaat gtgcttgccg atgagggcat gtggatgctt
     6301 aaccttatag gtaaaaacat ttcggcaatg caaacgctag gactaaaatt gacagctgag
     6361 gatatttaca gtgttaataa atcgagccta aaggatgcca tagttcaatt cgatgatggt
     6421 agctgtagtg ctgaaatggt ctcgccaaat ggtcttttct tcaccaatca ccattgtggc
     6481 gttgatgctg ttcaggaatt aagcaccgtt tccaacaatt tgttagagaa tggttactgg
     6541 gctaaatcgt atgctgaaga attgccggct aaaggcaaaa cggcgcttat acttgttgac
     6601 attaccgatg taacctccga tattcttacc ggcatcagtc catcgctttc caacagggaa
     6661 tactttgaag ccattaacca gaagatgcaa taccttcagg attccgttga agcaaaaagc
     6721 gggtatcacg tggtggtaag gccgtttttt aactacaatg agttttacat gttccgttac
     6781 gagcgttacc ttgatgttcg tttggttgga gttcctcccg catcaattgg aaattttgga
     6841 ggggatgtgg ataactggca ttggcctcgc catacaggcg atttctgtat atttcgtata
     6901 tacacctctc ccgatggtaa acctgctcag tacagcccaa aaaacattcc gttgaaatca
     6961 aagaattact taaaaattaa cctgcaaggg gttcaggagg gcgattttgc catgataatc
     7021 ggatttccgg gaagaacgca ccgttatgct tcgtcctatg aggctatgtt taaccgcgag
     7081 gtggtagcca actttaagcg taccgtttgg ggggaaatga ttaacgcaat taaaaaagcc
     7141 caacaaaccg accccgcaat taaggttaac tataccgata agcacgacta tcttataaac
     7201 ttttaccaaa aagatgtttg gcaagccgag tcaatgtata agtatagcgt tgttgaacgc
     7261 cttgctgccc gggaggatag cctaaaggct tgggctaata gcaatccttt tctttacagc
     7321 cgctatgtta ccagtatccc agtattggaa aattactacc agactgtaac ccaaaaccgc
     7381 tgggaggagc ttgagggagc catttcagct ctgtcgtttt acccggttga cgtatataaa
     7441 aatgttaccg cttgcaacga tttcgtttcc gcaatcatca tgcagggcaa gccctacagc
     7501 cgattaagtt ttaaaaggga ttacattcgc caggaggcta aactgcttga aaaacgatta
     7561 ccaaaaatct ttgaaaaata ccatatttac cccgatgttc agctatactc tattgctttt
     7621 ggcgcactgc taaacaacag tggtgaattc aataatttgg gaatgcttaa tgccattaaa
     7681 aaagtcgaag atattagcac ctcttatcca tactatataa tggctttctt tgagaaatcc
     7741 tatttcacct cgcctgaaaa tctaaagaaa ctgattaaaa gaccttactt ggattcattg
     7801 gtaaaagatc cgctcttcat cctataccat aattacaata tgctttggga ttccatatac
     7861 aactccatgt atgaggcaaa gcagaactac aacagagcaa tgcagctcta taccaaagga
     7921 ataatggaaa tgaacaaggg taaactttta taccccgatg ccaattcaac accccggtta
     7981 acctatggga aggtaaaggg ttatcgccct gccgatggga tggcatacaa accctttaca
     8041 acccttgatg gagtaatgga aaaggaagat cccaattccg acgtgtttaa agtgcctgaa
     8101 aagctacgta agctatggga acgtaaggat tatggcccgt atgctaggaa tggcgtaatg
     8161 ccagtttgct tccttaccga caacgatatt accggtggta attccggtag cggagtgctc
     8221 gatgctaatg ggaatttgat aggaatagcc tttgacggca acgctgaagc catggcctgc
     8281 gactttatgt acgaacccga aacccaacga accattgtgg ttgatattag gtatgtacta
     8341 tttattatcg ataagttcgg tgaatcaaag catatcatga atgagttaac aatactctaa
     8401 aaatccatat ctcttaaata aaaactcccg cagaaagcgg gagtttttta ttatatgctc
     8461 ttagtggtta cgctcatttg aggcgggtta tgcaggtgct ttttaaccgc acactggtcg
     8521 gccgccttaa taacggcctc cttgtatttc tcaggaaaat cggcaggaag gttgatttcg
     8581 agttccaacc tttcaatcat gtgtgtcaag gggttaaact gatgcttttg cacaatttta
     8641 atattgtcgg ttggtatgcc ccgctggtca cagaacgatt ttacgtaaat acctgcacag
     8701 gtacctattg atgccaaaaa taatgtgaat ggagctgggc cttggttatc gccacctcca
     8761 taaaccggtt gatcggttaa aatatcgtgc tctccaatgt gagcaacaac ctgctttttg
     8821 ccttgaaatg taacttcaaa ttccatatgt tttaattatt ttttagtttt gcaaaattat
     8881 aacacaataa catatgtaaa tgtttacata tcatataaat tatttggaaa atttctaaat
     8941 agtgttaatt gcaggttcat tattaagtgg aaattcttac cgagtgatag gattttgaaa
     9001 ttaattgcca ttttgcatat tcggttaata gaaaagaaaa ggggtgccga aaatcgacac
     9061 ccctcttcgc ataaaacact ctagcagcta ttactttttg ataaattgtt ttgttatagg
     9121 ctgtttttca tcgtcaattg agatcatgta aacaccagga gccaaatcat taatctcaat
     9181 tgtgttattt ctgtcggtta gcatctggaa tttaacaacc gcgccacgca tatcgtatat
     9241 acgagccgat acttcacctt caatgccgtt aagaacaaca tttatctttt cggaagcagg
     9301 gttaggatag atggtgaaaa tatcaacctt ttcgttacca agctcctcag caaaaggatt
     9361 gtcggttgaa ggagctgatg ttccgcttgt aatatttacg ctgtagtcct caacctcgcc
     9421 gtagctaaat gtttcacaag cggtaggtgc agcattgtat ttcatgctta cacgcatgcg
     9481 agtaacgcca agggtagcag ttgtaggaat ggtaatacta gccgaaaggg ttccgctgct
     9541 cgatgatgaa ccgctaacta ccttttcatt agtatcaaaa gtgccattct tgttgtagtc
     9601 aatccataca gcccagtact cggtatagga gctactcttg aatcctgcac tgaagtagat
     9661 tgtgtatgtg gttccgcgtg caacggtagc ctgcagactg gtcatgtcct tgtagccacc
     9721 atcgttgcct gaagtacgat tgattcctgc aaattgaaca aggtcaatcc attcgtagct
     9781 ggagttgcta ccctttgaag cgcagtaggt aaccgtgctg gtagttgtgg taaagctaac
     9841 ctggctaccg tacgatgtgc cttgggcgtt aattgcgtat gcacgagcgt agtatgtggt
     9901 gttagcagcc aaaccactga ggtttgcact gaacgatcca gtacccgaac cacttgctac
     9961 cttgctattt gctgtggttg gattttgtga tgtgctgtag caaataccgc gttcagttac
    10021 agttgcacca ccatcggctg ttacattacc ggctaccgtg gctgtgctgg aagtaatgtt
    10081 acttacactg ccagtagtaa ccgttggtgt agttgctccg ctacctgcgg caacagaaac
    10141 attgtcaata taccagtagt caatgttgta gagattacct tcggcaacaa aagctatata
    10201 tgttgttgca gagttgaggt ttgttgtaat gttaacagta acactagttg cgttaatatt
    10261 ggtgcttgag gtagccactg accaagatgt gttggtccaa ttggtcttat cgttcgaggt
    10321 ttgaactctc aaagtaacac cggtgctgta tgcatcaagc atatggcgga atgttaatgt
    10381 tacctggctt acacctgatg tgtttatggc aggtgtgatc aggcgtgtgg tgcccgggtt
    10441 aacattctgg taagtacatt tcatttcgta ggccgatccg ccagccttat tggtgttgga
    10501 aacgctccaa cgttcagtta taccgctacc tgtgttctga gttgtccatc ctgttggcat
    10561 cgatgagcta ctaaaggttt gggagtacgg aagagtaact gaagtggggt taacagcggt
    10621 ggtaaagttc atggtgctgc cataggtggt tccgttggcg ctaacagctt taaccctgta
    10681 gtagtattta gtatttgctg ataaaccggt aaggttagcg gtaacgttgg tatttgaagt
    10741 tccggtaaca ctattaggcg tagcattgat gctgttacct aaacttgttg tagtacccca
    10801 ttcaaaagta acagtggttg tagcattatt ggcattaaca gtaccattca aagtagcgct
    10861 gctggtggtt attgatgaag cggctacagt ggttgcggtt ggtgcagtgc ctccacctga
    10921 tccacccata aatgcaaagg tgatatcctt ggtagttgca ttctcagcaa tactggtcat
    10981 aggcttgccc gtattagcgc cagcccacga tttcatgtta ggggtagtag catcggtaaa
    11041 tgatgttttg ttgctacttc cggggaatgg gcaacctgtg ctattaatgc taccataggt
    11101 acttgatgta gtgccagggt ttgttgttgc gcttgcgcaa accggataca ttttttgtgg
    11161 gtaagtagcg ttaatgttat tgctgctgct tgcactggca acatccttat gaacatggta
    11221 aattatcaag ccatggccag gaacgtatgc atcaaaacct acttgctggc ggttttccat
    11281 aatccagtac tcaccgctgg tagtagagtt aatctggaag aaactcttgt tgtctttaac
    11341 tggtttaact gttacgtttg ttgccgatga aagaacggtt gcagttgccc atccgtaaac
    11401 cttaacttta gtgtatgcgt tgtggtgagc gggtgttacg ccattgttgt tccatgagcc
    11461 gctggccatc atatcccact tgcctgttcc ctcaaactga ccaccggtgc tataatcggt
    11521 atcatagtag tcaggagcgc caagtacatg accaaactca tggcatataa caccaataga
    11581 tgtaatagtt gttccggagt ttcctcttag ttctgcagag catgagtatt ttgaaatagt
    11641 tttcccgtct ttggtaacag gagtaatact ccatgcgtgc gcccaaatag catcggcact
    11701 ggcacctgcc tcttcaccat aacctgcata aataacatac actccatcaa ctgtaccatc
    11761 gccatcatta tcgtagttgg caaagttagc tgaggcgtcg gctgcattaa cggcttcagt
    11821 aaccaaagct cttgggttct tatcgttacc gtatgcatcg ttagctccgt agtacgacat
    11881 gttctggctg gcagtatagg ggccaaatac atcaaccgtt aggttgaatt ggccccatga
    11941 gttctcaagg tagtaatcct taacgctacc ggttgcaccg ccagtgctat atcctacctg
    12001 gttgaatagg ttgttaaaat cactttgggt tttagtaaag gctttatcct taaaccccat
    12061 cagaatacat attagctttc tattgccggt tgtggggaaa gctttctgtg cctcagctga
    12121 tttaatgtcc caaatgctct tcatcattga tagctggctt gggctatagt ataaaccctt
    12181 acttaccttt gagagcagtt ccttttcagc agcagttctt tccttgatgt tgtttacctc
    12241 aatgcctgaa agctgcaaat cgcccttcat gtcctgaatg gcatactcat aaactccatt
    12301 gctgtttagc ataatggtgt aaccatcctc agtggttgcc cacttaacct tttcatcacc
    12361 ctttaggaaa atggccagag ttttaccatt agcctgtttg tactgtacaa gtcccggata
    12421 agccggtact gcctgggttt gatttaccca tagcaggcat gtcataagac aaatgcttac
    12481 taaaccaagt agattttttc tcatagcgtt tttttgttta ggttggtgtt tagttggatg
    12541 cgcgcaaggt atatacaata ggtgttaatg tcaatatttt tgtactcttg aatgtgctta
    12601 tgtcgacata aaacagtttt taacatacga aatgcaattt caaaagcata taatgttaaa
    12661 taattgtata ataatgcgtt atatgatttt taatattttg ggtactaagt agggaagtgc
    12721 attatgctaa aaagtattaa attgcgaggc taaactgtag gttacaatgg atttaaaggc
    12781 cattattgtc gacgatgaac ccttagccat tgcggtaatt gatggttacc tctcccgtat
    12841 acccggggta gaggtagtgg ctacctttaa cgatggactt cctgcttttg agtatcttca
    12901 ggaaaaccct gtggatatag tttttttgga tgtagaaatg ccaaagctta cgggtattga
    12961 acttgtacgg tgcttgtata acccacctgt ggttattata acctcggcaa acagggacta
    13021 cgcaattgag ggctttgagc taaatgttgc cgattatatc cttaaaccag tcacttttga
    13081 aagactactt aaagcattct ctaaggctaa agagcttctt tcaaaaagag ccgaaaatac
    13141 aagccctaag gactcggaat accttatatt taaggagagt agaaagagcg taagggtaaa
    13201 gcaggatgaa atactctact ttgaaagcat aaaggactac gttaaagtag taacacctaa
    13261 cagaacagtt ataaccaaac agagtataag tagcttagag gagttgctcg ataaagagaa
    13321 gtttattcgt gtacaccgct cttttatagt tgcacttaat cgtgtggata ccttttccac
    13381 cacctcgcta gaaattggag atattgaaat acctattggt aggctgtata aggaggatgt
    13441 gattaaaata ctggaaacta aaaaataaaa ttttgtgtta cggagattga aatatccgtt
    13501 actgatcatt gttctagttc agcttacctt tgccaatgca caggagcgtg gattgttgct
    13561 ttcccattac tattcagcaa atacctatca ggctgctacg cagaactggg gtatagttca
    13621 ggataagagg ggtgttatat actttgcaaa tggagcaggg gtgcttgaat tcgatggaaa
    13681 gaattggcaa tttattgagg ttgggaaagg tgcctcggtt aggagtattg ctattgatag
    13741 taacgatcga atttacgttg gaggttacgg tgaatttggt ttgttaagtc cggataaaac
    13801 cggcttgttg agatatgtgt cgctttcatc gcatgtcgat tcagcatata aggaattcaa
    13861 tgaggtttgg gataccaggg ttattgccga nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13981 nnnnnnnnnn acctttaagc ccgataggtt taaccgtttg gagggcaggt acgaacaggc
    14041 ttggcagttc ctttactttc aagaaccatc cgatacgacc ttacttaagc cactttggta
    14101 tggtagtaat cagaagaccc acttgctggt tgctgcaagg aatggagttt ttgaaattaa
    14161 taaaaggagt gccagtaaaa ttacccggct tgagctaccc tatgtccttc atcagggcag
    14221 gatcaatcgt tcataccttt ttgtggggca tgaaaacgga gtctcccgct tgaagtacaa
    14281 gaacggaagg tggttaaatg aggctttgct ttgcaaaaca aaccgtaaaa tactaagtat
    14341 tggagaagat agcaatggaa atgtttggtt ttcagagaat ctaggcgggg ttgggtgtat
    14401 tgtgaatgca cagcttgaga aaagtgagga tgaagtattc aggtacgata cctctaatgg
    14461 gttgcccgat gcggcctatg ttagaatttt tgatatttac gacaccatac tctttattgc
    14521 taacagcaag ttttactctt ttaaccaaga tgcgaaaagg tttgagcctt ttgacataaa
    14581 atcgttaaac attctggaat ggttgccaag caacaacgac accctaatgt ggcgaaggat
    14641 aagcaaccag cttttaactg accaatatgt tacccatttt gcagactcaa tatcgtgggt
    14701 gtccaccgac aagggaatct ttcaggttcg gcctgctaag gggtataact actttaacct
    14761 acctgcccca attatttata gggcgataaa tatggactct gtgctcttta atggtaacaa
    14821 ctatagcctg gttgaaggtg atacagtttt tggagcgtat ttgtacaaaa aaatagcaga
    14881 tcctatcatt gatttaggaa tagtacttcc gtacaaaagg aattctgtga aattttcata
    14941 ctcatggcca tactttgaat ccgatcagcc ttgtcaattc agttaccagc ttgttggtaa
    15001 cgatgaggga tggtcagagt ggtcgactga ttaccggaag gagtatacta atttacgtga
    15061 gggtaagtac gtatttaagg ttaaagcacg aagtgttttc ggggttgaaa gtcctgtttt
    15121 agaatttcgt ttcgaggtta aaacgccatg gtacagaact atttacgcat acattggcta
    15181 cgctttgctt gtttttttac taatatattc gatatttaaa atttggcatt acaagttaat
    15241 actggagcgc gacaagctgg agcgcttggt taaggaacgc acacaggaaa ttcttttaca
    15301 aaaggaggaa cttcaggttc aggcagaaca tctcagggaa acctacgaat ggatatcgga
    15361 aaagaatgcc atgctcgaaa agcaaaagag tgagattgaa caacaaaaac aaaagctcga
    15421 agaaattaat gctacaaaaa acaaattctt caggattatt gcacacgatc ttcgcaaccc
    15481 tattagcact ttggtaaact ctactgcctt tcttttaacc gatattgaaa cgttaagttc
    15541 ggaaaaaaca aaaaagttta tgggggaact taataggctg gcattaacca cctatgggtt
    15601 acttgaaaac ttactcgact ggtcatcgaa cgagatgggc gacatacaaa ataaacccca
    15661 aagggtttcc cttgcgcttt tggtaaacca aaatattgag ttagtacaga atcgactaac
    15721 agacaagaat atcaagttaa ccgttgatat cccggctagt attgaactct atgttgatga
    15781 gaatatgctg aatacagttc tgaggaactt aatcagtaat gctgtaaaat ttacaagacc
    15841 tggaggtgaa atttcgattg atgcttcaga agttaatggt aagtgcgtaa tcagagttaa
    15901 agataacgga attggtattc ccgaccaaaa tatcgaaaaa ctgttcagga ttgataaaag
    15961 cattgtaaca cctggtaccc aaaacgaaaa gggttcgggg ttaggattac tactttgcaa
    16021 agaatttgtt gataaaatgg ggggaacaat atcggtaaat agcaggcagg gctttggaac
    16081 tacattctcc attgagttcc ccgtagatat actttagctc atctattcaa ataattttag
    16141 ccggttaaac cttatacgta aggcaaaatc aaacttaaca aaccaaaatt catagttatg
    16201 aagcggataa agtacttttt cagccttttg ctattcctaa tcatggcgat tggggtagtg
    16261 tttgctcagg ataaaaagga cgagctaaaa gagccctatg cctttaccga tgttaaggtt
    16321 atacccacaa catcggttaa gaaccagaac cgctcaagca cctgctggag tttttcggga
    16381 atggcatttc tggagtcaga gcttttacga atgggaaagc cggccgttga cctttcgcct
    16441 atgtttgtgg tcaggaatat ctacgccatg aaagccgata ggtatgtcag gttcaatggt
    16501 acaaataatt ttggacccgg tggttcattc ttcgatgtgc tggaagccat taagcggttt
    16561 gggatagtac ccatggaagt gtatcagggc ttaagctatg gggaggaatc gcatgtacac
    16621 ggcgaggttg atgccgttac taaggctttt gtagatgcaa tagttaaaaa tccaaaccgt
    16681 aagctctcca ccgcttggaa aaaagcattc gatggtatac tggatgccta ctttggttca
    16741 ttaccccaga attttaccta caatggcaaa acatacacac cagaattgtt tgcaaaacag
    16801 ctaggaataa atccagacga ttacgtggta ataacctcgt tcagccatca tcccttttac
    16861 cagaaatttg ttctggagtt acccgataac tggatacatg gcgaggctta caatgtgcct
    16921 ttaagtgact ttgataggat tattgaaagc gccatcgata acggctaccc ggttgcctgg
    16981 gcaagcgatg ttagcgataa agggtttaac tggagtaagg gtgtagccat tgtacctgat
    17041 tttgatgaca aggaaaatgc cggttccgac aaggagcgct ggactcaact ctcgaatcgt
    17101 gagaaggaaa aaactttata ctcgttcgag aagcccgtta aggaaatggt aattacccag
    17161 gagataagac aacttgcctt tgataactat caaaccaccg atgaccatgg tatggtgctg
    17221 gtaggcaagg caaccgatca gctggggaat gcattttaca aagtgaagaa tagttggggt
    17281 gccgatggta aatacaaagg atatttttat gcctcaaggg catttgtact atacaaatca
    17341 actaatatta tggttcataa aaaagctgtt cctgttgatt tagcaaagaa attgggaatt
    17401 tagtaaatac accgaacaaa tttatccgcc catcgtttaa ctattttggg cggatttttt
    17461 tatttgtatg gtataatatt tataaattta catgctgata aagacttagg ccatgacaaa
    17521 tttatacatc aagggcacta ccaaaacacc ggagattgat tttaaaccgg gtattctgca
    17581 aatttcagga cgctcaattg ctgaggatgc agttgctttt taccagcctg ttattaaatg
    17641 gattgaagat taccttaaaa agcctgaacc attaaccagg ctcaacctga agatggagta
    17701 catcaatagc ggttcaaacc ggtttatttt caccataatg aggatgcttg atgaggccta
    17761 ctcacaggga cataatgtgg ttattaactg gtactttgag gaggatgacg acaccattaa
    17821 aaacttaggt cgtgattttc aggccctggt aaaggctcca ttcaaaatgg tagagattat
    17881 ttaatcgtca tcatctacta cttccctggc atctatttgt ggggtgttag tatcgttaaa
    17941 gttttcagcg ggaaagtgaa caactagcca ggctatatcg cgaacaaaat caaccttgcc
    18001 aatttctaca ctcttaattt caaggccagt tcgtttcttt agatcctcta taagttctgc
    18061 ccttttatcg ggggtaatga ggttgatttt ctcgtaaact atcactttct gtgatagatg
    18121 ttttatgaaa aggccttttt caagtattag agccacgcct aatagcgcaa aatttgaaaa
    18181 taacagttct gcatagctaa cctttttcga cgatatggca tttattaacg atatggcaat
    18241 tatgaaaaag agataagtca tctctttaat tggtattggg ttggtccggt agcgaataat
    18301 cccaaaaaca gcaaataacc ccaaagcaaa accaagctgt aacttgacgt ttgcaagcag
    18361 aaagcacata aaaaatacaa ccacgccaag cataaggtat gtaaaaacat aatctttacg
    18421 gtaggtacgt gggtagtata ggtaacggac agcaatccat aaaataacag tgttcaggat
    18481 aaaccgtagg agaagtgtgt aaaaatcgtt cgtgtttatg atatcgattc caaatagctg
    18541 gaacgaatca gcttgtagtg ttaaccaaag cattttcatt tttgtacttg ttaatgtgcg
    18601 taattattgg cttaaagtta ttggacttta ggttattata caaaagggcg cacccaattg
    18661 agtacttgct aaaaccggtt tgtctatatc cgtgttgctt taaatgattt ataagaaagc
    18721 ttgacgagtt gtacttgtcc cgttttgatt ctattacaca aatatctgaa aggtttacgg
    18781 catgggttgg atttttgagc tgtatattga agtccattgt aacacgttca ttaaaatcga
    18841 acgatactag ggttatcctg ttaaaggttg tatacaatgt tcttgttaac tcattaaacc
    18901 tgaaaggaat atttttttta acaaaggctt caaccgttgg attatcaatg gaattgttgg
    18961 taatttcggt tcgtttcttt aatgtttcgc ctgaaggcat tcgtgttttt acctctagga
    19021 atacatcacc actagctctg taatgcctga gccttacctt ataacgctta gccctcctgt
    19081 tatggtggtc aagatacatg gtaaagccac tgctgtcaaa atattccgaa aagtactcag
    19141 gcattagctg tgctttattc tcaacaataa agtagtgttg ttttaagaat gaaagtattt
    19201 caaatgccca ttctgcctgt actaaatact tactatcgat acggcgcata aattgcgcct
    19261 tgcccacatc gcctaggttt aagttgttga aatctaattg tttaatatca ttctctttca
    19321 tggattacac tatactactt atattcaaaa actttcctac ttttcagttt tccattaggc
    19381 ccatatactt tcttttc
//
LOCUS       DAOSAL010000049        19352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_1989_length_19352_cov_49.139193, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000049 DAOSAL010000000
VERSION     DAOSAL010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 19352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19352
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1989_length_19352_cov_49.139193"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2478
                     /gene="clpB"
                     /locus_tag="PK049_07730"
     CDS             <1..2478
                     /gene="clpB"
                     /locus_tag="PK049_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOK61486.1"
                     /translation="ILDEGENITGFLLRKLGVNPGTFDAVLNRMLDSMPKVSGGEQFL
                     SSATNRVLQKALEYSKAMGDQFISVEHILLGLVAAGEQVGQLMKDAGITEKELKQAIA
                     ELRKGAKVDSQTAEDTYNALNRFAINLNEMARTGKLDPVIGRDEEIRRVLQILSRRTK
                     NNPILIGEPGVGKTAIAEGIAHRIVNGDVPENLKTKQIFSLDMGALIAGAKYKGEFEE
                     RLKSVVKEVIASEGEVILFIDEIHTLVGAGKSEGAMDAANILKPALARGELRAIGATT
                     FNEYQKYFEKDKALERRFQSVVVDEPQPADAISILRGLKERYENHHKVRIKDEAIIAA
                     VELSHRYITNRFLPDKAIDLIDEAASKLRLEMNSVPEEIDELERKIKQLEIEREAIKR
                     EGDKPKLEELSRTLAELNEERTRLRAKWESEKNVIDRIQQLKQSIEQMKYEAEDAERN
                     GDYGKVAEIRYGRIKQAEAEIEHLKGELKKVQADFALIKEEVDAEDIAEVVSRWTGIP
                     VSRMLQSEREKLLHLEEELHKRVVGQDEAIAAVADAVRRSRAGLQDAKRPIGSFIFLG
                     TTGVGKTELAKALAEFLFNDENLMTRIDMSEYQERHSVSRLVGAPPGYVGYDEGGQLT
                     EAVRHKPYSVVLLDEIEKAHPDVFNILLQVLDDGRLTDNKGRTVDFRNTIIIMTSNIG
                     SHIIQENYAQLSKSNAEQVFERTRNQVFELLKQSIRPEFLNRVDEIIMFTPLNREEIK
                     QIVLLQFERIKQMLAQNGVGIEISESAVEWITNIGFDPVYGARPIKRVMQKKILNELS
                     KQLLAGTINKEQTITVDYFGDDILVFR"
     gene            2693..5896
                     /locus_tag="PK049_07735"
     CDS             2693..5896
                     /locus_tag="PK049_07735"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOK61487.1"
                     /translation="MAGLLVYKASAGSGKTYRLVAEYIKMLIENPQAYREILAVTFTN
                     KATAEMKGRIIEKLYKLKKSEEPDLLQTLKAETDFNEDKIRSRAEVALSNILHDYSMF
                     SISTIDSFVQRIIQNLLWELGQHGNNELVLEHKPYIEKASDTLLDELTKHPELFNHVK
                     RLIEERLDENKSPNIQNDLINLGNMLFHERYKLLSSEERELMHNKETLNALKAEVNRI
                     ISEIYHGIKTPAGKLLEQIYSSGIDGINQDNYSDFFKKKGGIIPVIAKLAELKRLTPI
                     ELKSIAEDALSDISVWLTKEKISMLKGFVEQQLMPQYRELYSYLNYNYRLYNTALAIN
                     ENISTLYIINELYKKLREKLNDDGVMLLSDSSALLKEFVSQTDTPFIYEKIGTKYTSY
                     MLDEFQDTSQLQWRNFEPLIADSLGSSGLSMVVGDVKQSIYRWRNSDWRIMAGIEAER
                     QFYPQVKQLTVNYRSARTIVEFNNHFFQKAIEINEWENTINPQNPAFNLKNLYSDVKQ
                     EVKKLEPEGYVEVAFMPPKTDEANDYFTNHIRNILVDLKDRGYRAGDIAFLVRKNEQG
                     RQLAELLLQLSKSDTQLSGFIKIVSQDALILNSSAAVRLCVAALKIALNPKDTLAVAQ
                     FRKEFALLKNQHATIHWPSIFLPDSDDTFDWLSSIRFYPLGYMVEAIIQKYFHNQYID
                     INDHLPYLSLLHEYAIDFSYRGTSSLSRFIEWYEQDGKGKHLFMAETENAINILSIHK
                     SKGLEFPIIIFPFADLQNQQKQQDSIMWFKLPADTPNEILKNYPLLPIKPKKALAQTY
                     FEADYYDEQFCKEIDDINLQYVAFTRAKHELYILVTDKKLAEDEINKTQENVEQNKFV
                     KFLPNSSNLSPQKVVDGKKVTFTYGQKSTKKVDLNDSMGEETKLIKHYPINPMRGKIA
                     QQFKVDSGEIDQKGDLQHGIAMHAILSKIITLDDLDEAMDWAVQNGYITANQRQNVHN
                     SLISMINSEPYKQWFSSTWEVKNEASIVNTDGNIYRPDRVLIGDNQAVIIDFKFGLAT
                     PKHRKQVEQYKELLLSMGYERVKGYLWYFQLNRHEEV"
     gene            5968..7350
                     /locus_tag="PK049_07740"
     CDS             5968..7350
                     /locus_tag="PK049_07740"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HOK61488.1"
                     /translation="MRKYLIYLTIPAMVACGSKEPKPTTLAGRYTNGTDNKVIVISPD
                     AIDTISLNENGLFVHKVNLANPAMLKVRSGRIQTTLYLEPGDSSYFEVDANNADGISF
                     SGSNSELNQNLRSRSKELQNIFRGWRDLFSLNLNEFGLKIDSISKELFQKTDSLKAKS
                     KNFAEMEANRVRYFMLRLRSQYPEYSAYLSGEEFNPDSADYSFFDNVDMNNGEHLSYD
                     EYASLVETYAQLRIQKAQNFKELSSKPAQERLPAIFSMIDSIFTNPKIRDYVKKQYLM
                     EEIQFGEFWKLNDICNNYLAACTTPVYKQEVQSVYNEKLKIAPGNSAPTFTYNDINGK
                     SVSLSDFNGKLVYIDFWATWCGPCRGEIPHLDKLEKDYKGKKIVFVKISLDDDMDAWR
                     KMVTEKKLGGVQLHADGAWSSDAAKNYQIKGIPTFVLIGANGKIINPNAPRPSSEEIR
                     TLLDQELAKI"
     gene            7442..8398
                     /locus_tag="PK049_07745"
     CDS             7442..8398
                     /locus_tag="PK049_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444280.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HOK61489.1"
                     /translation="MTKVYPWGDTRRYNSYSGYFKREFGGRVQKLTIDAGFTCPNRDG
                     TVGLGGCTYCNNDAFNPSYCTPQKSITQQIEEGIEFHAVRYRRADRYLAYFQAYSNTY
                     APLEKLRALYSEALSHPKIIGLVIGTRPDCVDEAKLDYFQQLNSKYYIALEYGVESCY
                     NRTLQLINRGHTFEQSVWAINETSKRGIRVGAHFIFGLPGETQEQMLAEADIISKLPL
                     HTVKFHQLQIIKGTIMEQQYTTNPEWFKLFSMDEYFNFLADFIERLNPNFVIERFTGE
                     APPRYLAVPPWGNLRTDQLMIAFEKVLEKRDTWQGRLFESKV"
     gene            8484..8963
                     /locus_tag="PK049_07750"
     CDS             8484..8963
                     /locus_tag="PK049_07750"
                     /inference="COORDINATES: protein motif:HMM:NF018596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK family protein"
                     /protein_id="HOK61490.1"
                     /translation="MKRVSILIAITLAAFTAAIAQPKALKNFDELMAALNTGYNVRVV
                     IHYAKCTLVADNELQDKSPDAIGGMTIDTYEYFAAKAVRGNEHAFVVFSENKLIQNPK
                     GKGFVYNYAKFKVEDTGKVKVTVMYLDPNTQEELMSENFFTQFGDSTKEQAIYFYRL"
     gene            8972..11818
                     /locus_tag="PK049_07755"
     CDS             8972..11818
                     /locus_tag="PK049_07755"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOK61491.1"
                     /translation="MKPFLETVAEHLYSTHGNDISKLTLVFPNRRASLFFSKYLSKQI
                     AKPIWLPETTTISDLMFSYSGVKPADPLMLNYKLYKAYCQATHSLEPYDEFYFWGNVM
                     LSDFDQIDKYLVDPKKLFTNINDLKEIEQQFNEFDKETIELIEKFLNLVSSEGESLIR
                     SRYSKIWANLYGIYSAFTNSLISEGIAYEGLAYRLAVKALEGGTSAIPNQKFVFIGFN
                     ALSKSEQRLLLLLKSGERALFYWDYDNYYINSPTGVDHEAGLFMRQNLKLFPNALNNT
                     NFDFIGNIGSSKKVTLISSPSEVTQTKIVPDILDELNINNTNELTTAVVLPDEKLLLP
                     LLTALPEKYSNANITMEYPLRETSAYSFIDALLTLQKSSDKQTKFYYRDVLLILSHPY
                     IQLACPDEANRIRDIIIDRQLLNIPPDTFSGSDILEQIFKPVTKGSELANYLATCINS
                     IISRLTHEIDANDDKLKFEVEYLLTINRALKRLGLVISEINEEYSNKTFRSYLTKALA
                     EQRVSFIGEPLSGIQIMGFLETRNLDFKNLVILSVNDNLLPGSHFSPSFIVPTLRQAY
                     GLPDYKHQNAVYAYYFYRLMQRAENIYLVYTNITEGLKSGELSRFVKQLQMESNLNIK
                     EVTVNFDLGVNSERPIEVTKNEAIAKRLSKYFETTEGSRYLSPSALSEFKNCSLSFFF
                     DRVLGISAPDELDEELDAMGIGSVFHKAMEQIYSGFVGKEVTKDQVQNRLNQLGLVEE
                     TVFRAFKEISKFNGNIDELNGRNRLLLERVNWMVRNALSSDLNRTPYKLLAIEEEVET
                     VIPIELNGKALNVRLGGKIDRLELKNGKLTIVDFKTGKHEPAKTKISNISDFDGKTDK
                     DGLFQQLVYKFIYTQENSLGIGDVDILLWYVRSTDGIPSTEVPDTDEFWEEFISYLKS
                     LIFKIVDDSPSSQTSNIKLCENCSYNVICNRKVM"
     gene            11899..12618
                     /locus_tag="PK049_07760"
     CDS             11899..12618
                     /locus_tag="PK049_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61492.1"
                     /translation="MKKLTLSILILIAALSIKAQEVATTQNKINLNFKVDMVSRYLWR
                     GLLLDNNPCIQPDLSLSVGGFTFGSWASYGYNSKFPEIDFYLIYEFGPLSLTVFDYYN
                     EDETDLSSIKYFTWDRKLTGHALEGTIAWNGTENFPLRALAATFFYGNDRDADGNQYY
                     STYFEIGYPFNLSDYSLEFFLGATPAKGLYNNSANIVNVGFSASKDIKLTENFTLPIK
                     GSFVVNPAQENVFFVVGITLQ"
     gene            complement(12692..13243)
                     /locus_tag="PK049_07765"
     CDS             complement(12692..13243)
                     /locus_tag="PK049_07765"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOK61493.1"
                     /translation="MNEILQSIQQRRSAMAFSPEPIPNEHLIQLFRAAILAPSAYNEQ
                     PWLFYVANRENATAFENVLSALAPANQEWAKSAGALVVTAARKNYTRTGESNYYALHD
                     LGMATAMFLVQAQSMGYVTHMMGGFDHISIRRAIKLDDGYQIGAVIALGKPGSIENLN
                     PQNKQRELSPRIRLDLEKVLRVL"
     gene            13542..14753
                     /gene="rocD"
                     /locus_tag="PK049_07770"
     CDS             13542..14753
                     /gene="rocD"
                     /locus_tag="PK049_07770"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HOK61494.1"
                     /translation="MTPKDYMERESKYGAHNYHPLPVVLERGQGVYLWDVEGKRYYDF
                     LSAYSAVNQGHCHPKIIKAMCEQAQKLTLTSRAFYNNVLGEFEEYVTKYFGYDKVLPM
                     NTGAEAVETALKLCRRWAYNVKGIKDGEAKIIVCENNFHGRTITVISMSTDPDSYGGF
                     GPYTPGFIKIPYNDIPALEKALQDPNVAGFLVEPIQGEAGVFVPDEGYLKKAYDLCKK
                     HNVLFIADEVQTGIARTGKMLACDHEGVRPDILILGKAISGGAMPVSCVLADDEIMLT
                     IKPGEHGSTFGGNPLAGKVAIAALEVVKEEKLAENAERLGKIFRDEMKSIKSEMIELV
                     RGKGLLNAVVIRNKPGKTAWDVCLAMAEKGVLAKPTHGNIIRFAPPLVITEEQLREAI
                     GLIKEAFKQFE"
     gene            complement(14806..15018)
                     /locus_tag="PK049_07775"
     CDS             complement(14806..15018)
                     /locus_tag="PK049_07775"
                     /inference="COORDINATES: protein motif:HMM:NF039796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6485 family protein"
                     /protein_id="HOK61495.1"
                     /translation="MECKKENNSKFCNCSYPCGKHGVCCDCLAYHRKNGELPACYFPA
                     DIERGYDRSIDNFIRVVKERGTGYLR"
     gene            15153..17207
                     /locus_tag="PK049_07780"
     CDS             15153..17207
                     /locus_tag="PK049_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61496.1"
                     /translation="MRKIFTLLTIATALLMAQCKQPEQKVDVSPISDATVKKVTAELV
                     KQHGDSQKERIERGVAQVAMLWRITDGTETDFEQFCTTQFVSDDAALEQLFNKLSYGF
                     EMLNGYFILLSKELMKPLHLDWGPVTPIDELFGSYNAAAHLNDDLYANKIAFITALNF
                     PYYTLKEKTEMGENWSRKQWAYARMGDMFTSRVPAQLIQNFSTVNTNADTYISEYNIY
                     MGNLVDDKGQTMFPADMKLISHWGLRDELKSHYANKEDGLAHQQMIYQVMKRIIDQSI
                     PQQVINSNEYKWNPYTNTVTKEGNEVQATPEPNTRYQHVINQFKALKAIDEYTPKMPT
                     YIQRKFEGEFELPVDEVEQLFIDLVSSKEVREAGKLISQRLGRPLQPFDIWYDGFKAR
                     SGIPAEKLDGITTKRFRDAKAFEAELPNILTSLGWSKEKAKFIADRVKVEASRGAGHA
                     WGAEMHNDVALLRTRVPASGMNYKGFNIAMHEFGHNVEQTITLHDVDYYIMHGVPNTA
                     FTEALAFVFQKRDLDVLGMKEVNPDKEYLETLDNLWSCYEIMGVSLVDINLWKWLYNN
                     PNATADEVRNATLTIARDIWNKYYADVFGSTDEPILAIYSHMIDNPLYLSAYPIGHLI
                     DFQLEKQFRGRSFAAEVERIYSQGRLIPQLWLKKGVGEPLSVTPILEASAVAVQKLSE
                     KK"
     gene            complement(17273..18544)
                     /locus_tag="PK049_07785"
     CDS             complement(17273..18544)
                     /locus_tag="PK049_07785"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOK61497.1"
                     /translation="MFRYIDIRSYFNRVGFAFRALKSPNFRMFFYGQIVSLMGTYIQN
                     LALGWLIYRLTDSPFLLGLVGFAGQIPSLFLTPIAGVYADRVNRRYVLIGSQALSMVM
                     AFTLAALYLTGVLQIWHIILIAVINGVSLAFDTPFRHAFLLEMVGDRDLLQNAIALNS
                     TLINSARFIGPTIGGFIIALLGEGWCFIINGLSFMAVIASLMSMRVKPMNTHHHHGSI
                     FQELAEGLAYSYRNKTIRYLLLLISSIGFIGLPFQVFMPVFARDILHGNSQLLGFLTG
                     SLGAGALVGAFYLASRKSIRSLPKIILVSSILFGIGLFVFSLSSNTAVSLGVIFITGF
                     GMIVQFAATNTLLQHIVDEDKRGRVVALYGLSFMGITPLGSLLLGSISPTIGVQLTLA
                     IAGLLCLFAVVLFGRKYGTIAQTVKRFENSH"
     gene            complement(18548..18784)
                     /locus_tag="PK049_07790"
     CDS             complement(18548..18784)
                     /locus_tag="PK049_07790"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOK61498.1"
                     /translation="MAGYAIAGTIGFARVINNKHWITDVFAGAGIGILSTKISYYTYE
                     RFKLKQRSRGQMHAVIVPYFNGNGMVGLAAGIQF"
     gene            complement(18791..19288)
                     /locus_tag="PK049_07795"
     CDS             complement(18791..19288)
                     /locus_tag="PK049_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61499.1"
                     /translation="MILGILFYLNGFSQQLTRIDSLSQRPKLEYRLTVKNTFIPVSLI
                     TYGVVGLNNPKLKRWNTGIRSEVLEHINTKYRADDYFQYVPGLAVYILDDAGLKAKHS
                     FWDRTAILSGSFVLQLAVVRVLKHTTGVMRPDSSSTTSFPSGHTAMAFTGAEFLWQEY
                     KDHTV"
BASE COUNT         6040 a         4399 c         4113 g         4800 t
ORIGIN      
        1 atacttgacg agggtgaaaa cattaccgga ttcctgctcc gcaagctggg ggtaaaccca
       61 ggaacttttg atgcggtact gaaccgcatg ctcgattcca tgccaaaggt atcgggtggt
      121 gaacagtttc tcagcagcgc aaccaacagg gtgctgcaaa aagcccttga atacagcaag
      181 gcaatgggcg accagttcat ttcggtggag catatccttt tgggacttgt tgcggctggc
      241 gaacaggttg gccagctaat gaaggacgca ggcattaccg agaaggagct gaagcaagcc
      301 attgctgagc tgcgtaaggg cgccaaggtg gactcgcaaa ccgcagagga tacctacaat
      361 gcgcttaacc gctttgccat taacctaaac gagatggcac ggactggcaa gctcgacccc
      421 gtaattggtc gcgacgagga gatacgacgt gtgcttcaaa tcctatcgcg ccgaaccaag
      481 aacaacccca tacttattgg ggaacccggc gtgggtaaaa ccgcaattgc ggagggcatt
      541 gcccaccgaa ttgttaacgg cgatgtgcct gaaaacctta aaaccaaaca gattttctcg
      601 ctcgatatgg gcgcactcat tgccggagcc aagtataagg gcgagtttga ggagcgattg
      661 aagtcggtgg ttaaggaggt aattgcatcg gagggtgagg ttatcctatt catcgacgaa
      721 atacataccc ttgtgggtgc aggaaagagc gagggagcca tggacgccgc caacatcctg
      781 aaaccagcgc tggcgcgagg cgaactgcgt gccattggcg caaccacctt caacgagtac
      841 cagaaatact ttgagaagga taaggcgctt gagcgccgtt tccagagcgt tgtggttgat
      901 gagccccaac ccgccgatgc catttccata ctgcgcgggc taaaggagcg ttacgagaac
      961 caccacaagg tgcgcattaa ggacgaagcc attattgcgg ctgtggagct ctcgcacagg
     1021 tatatcacca accgtttcct gcccgataaa gctattgacc ttattgatga ggccgcatcg
     1081 aagctaaggc ttgagatgaa ctcggttccc gaagagattg acgagcttga gcgcaaaatc
     1141 aagcagcttg aaattgagcg cgaggccatt aagcgcgagg gcgataaacc taagctcgag
     1201 gaactatcca gaacactggc tgagctaaac gaggaacgta cccgtttgcg cgctaagtgg
     1261 gaaagcgaaa agaacgttat tgaccgaatt cagcagctca agcaaagcat tgaacagatg
     1321 aagtatgaag ctgaggatgc tgaacgcaat ggcgattacg gcaaggttgc cgaaatacgt
     1381 tacgggcgaa tcaaacaggc cgaggccgag attgaacacc taaaggggga actcaagaag
     1441 gttcaggccg actttgccct aatcaaggag gaggttgatg ccgaagacat agccgaagta
     1501 gtatcgcgct ggactggtat tccggttagc cgcatgctcc agagcgaaag ggaaaagcta
     1561 cttcaccttg aagaggagtt gcacaagcgc gtggttgggc aggacgaagc cattgccgct
     1621 gttgccgatg ccgttcgacg tagccgcgcc ggcttacagg atgccaaacg cccaataggg
     1681 tcattcatat ttcttggcac tactggtgtt ggtaaaaccg aacttgccaa ggctttggcc
     1741 gagttccttt tcaacgatga gaacctgatg acccgtatcg atatgagcga gtatcaggag
     1801 cgccactcgg tatcgcgtct ggttggcgca cctccagggt atgttggcta cgatgaggga
     1861 ggccagctca ctgaagcggt aaggcacaaa ccctactcgg tggttctgct cgatgaaatt
     1921 gagaaagccc accccgatgt gttcaacatc ctgcttcagg tactcgacga tggtaggctt
     1981 accgataaca agggtcgcac ggtcgatttc agaaacacta tcatcattat gacatcgaac
     2041 attggttcgc atattatcca ggagaactac gctcagctta gcaagagcaa tgctgaacaa
     2101 gtatttgagc gaacccgcaa ccaggtattt gagctgctca agcagagcat caggcccgaa
     2161 ttcctgaacc gtgtggacga gataataatg ttcactccgc ttaaccgtga ggaaataaag
     2221 cagattgttc tgttgcagtt tgaacgaatc aagcagatgc ttgctcaaaa tggcgtaggc
     2281 attgaaatta gtgagagcgc agtggaatgg ataaccaata taggatttga ccctgtttac
     2341 ggagcacgcc ccattaagcg ggtaatgcaa aagaaaatcc ttaacgaact ctccaaacag
     2401 ttgctggctg gaactataaa caaggaacaa accataaccg ttgactactt tggcgacgac
     2461 atattggttt tccgataatt tccgagtatt tttctgacaa gccgcctact tttgggcggc
     2521 tttttttata tttgaaaagg attagaaaaa gtgaatagct aaaagagatt cgttaaccat
     2581 tacactaagg aacaaagaac atgaggaagt tagaggatat taaaaaactg attaatgaat
     2641 aattttctgt aactcctaaa tctaaactcc aaattcaatt ttcttttcac atatggcagg
     2701 tttattggtt tataaagcat cggcgggttc gggtaaaaca tatcgacttg tagccgaata
     2761 catcaaaatg ctcattgaaa atccgcaggc ttaccgcgaa atacttgcgg ttacattcac
     2821 caacaaggcc actgccgaaa tgaaaggtcg aattattgaa aaactttaca agctgaaaaa
     2881 gagtgaagaa ccagatttgc tgcaaaccct taaagccgaa acagacttca acgaggataa
     2941 aataagaagt cgcgcggagg tagcgctttc caacattcta cacgattact caatgttcag
     3001 cataagcacc atcgatagtt ttgtgcaacg cataatccaa aacctgctct gggaactagg
     3061 ccaacacgga aacaatgaac tggttctgga acacaagccc tatattgaga aggcttccga
     3121 tacgctgctc gatgagctta ccaaacaccc cgaactattt aaccatgtga aaaggttgat
     3181 tgaggagagg cttgacgaga acaaatcgcc caacattcaa aacgacctca tcaacctggg
     3241 caacatgcta ttccatgagc gctacaagct actcagctcc gaggaacggg aactaatgca
     3301 caacaaggaa acgctcaatg cgcttaaagc agaggtaaac cgaattatct ccgaaatata
     3361 ccatggtatt aaaacacctg caggaaaatt gcttgaacaa atctacagct ctggcataga
     3421 tggtattaac caagacaact acagcgattt tttcaagaaa aaaggcggaa ttataccagt
     3481 gatagcaaag cttgcggagc taaaacgact tacacctatt gaacttaaaa gcattgcgga
     3541 agatgcgctt tccgatatat cggtttggct gaccaaggaa aaaataagta tgcttaaggg
     3601 ctttgttgag caacagctga tgccccaata ccgtgagctc tatagctacc ttaactacaa
     3661 ctacaggtta tacaataccg ccctggcaat aaatgaaaac atttcaacgc tttacatcat
     3721 caacgagctt tacaaaaagc tcagggaaaa gcttaacgac gatggcgtaa tgctcctatc
     3781 ggacagtagt gcgttgctca aggagtttgt ttcgcaaacg gacacccctt tcatctacga
     3841 aaagattgga actaaataca ccagttacat gctcgatgag tttcaggaca catcgcagct
     3901 gcagtggcgc aactttgaac cactcattgc cgacagctta ggaagcagcg gacttagcat
     3961 ggtggtaggc gatgttaaac aatcaattta ccgttggcgc aacagcgatt ggcgtattat
     4021 ggccggaatt gaggccgagc gacagtttta cccacaggtt aagcagctaa cagttaacta
     4081 ccggagcgca agaacgattg ttgaattcaa caaccatttc tttcaaaaag ccatcgaaat
     4141 caacgaatgg gaaaatacta tcaatccaca aaacccagcc ttcaacctaa aaaacctcta
     4201 ttccgacgtt aagcaggagg taaagaaact tgagccggaa ggctatgtag aggtagcttt
     4261 tatgccacca aagaccgatg aagccaacga ttactttaca aatcacatta gaaatatact
     4321 tgttgatttg aaagacaggg gatacagggc aggcgatatt gcctttttgg tgcgcaaaaa
     4381 cgagcaagga agacagctag ccgaactcct gcttcagcta agcaagagcg acacccagct
     4441 atcggggttt atcaaaatag tttcgcagga tgcgctaata ctgaactcat cggctgcagt
     4501 aaggctgtgc gttgctgcac ttaaaatcgc cctaaaccca aaggacaccc ttgccgtggc
     4561 tcagttccgc aaggaatttg cattgcttaa aaatcaacac gcaaccatcc actggccaag
     4621 tatcttcctt cccgatagtg acgatacctt tgattggctg agctctatcc gattctaccc
     4681 tttgggttat atggttgaag caataattca aaagtacttc cataaccaat atatcgatat
     4741 taatgaccac ctaccctacc tatcgctact gcatgagtat gctattgatt tttcgtacag
     4801 gggtacttct tcgctaagtc gatttattga gtggtatgag caggatggaa aaggcaaaca
     4861 tctttttatg gccgaaaccg aaaatgccat caacatccta tccatccata aatcaaaggg
     4921 attagagttc cctataatca tattcccctt tgccgacctg cagaaccagc agaagcaaca
     4981 agacagcata atgtggttca agcttccggc cgatacacct aacgaaatcc ttaaaaatta
     5041 cccgctttta ccaataaaac caaaaaaggc attggctcaa acatactttg aagccgatta
     5101 ctacgatgaa caattctgca aagagataga cgatataaac ctgcaatacg tggcttttac
     5161 ccgtgctaag catgagctat acattttggt taccgacaaa aagcttgctg aagacgaaat
     5221 caacaaaacc caggaaaatg tagaacaaaa caaatttgtg aagtttttgc caaacagctc
     5281 aaacttaagt ccgcaaaagg tagttgatgg gaaaaaggta actttcacat atggtcaaaa
     5341 gtcaacaaaa aaagttgacc taaacgatag catgggcgaa gaaacaaaac ttattaaaca
     5401 ttaccctatc aaccccatgc gtgggaaaat tgcccaacag ttcaaagtgg attcgggcga
     5461 aatagaccaa aagggcgatt tgcagcacgg tattgccatg catgccattc tatcgaaaat
     5521 tatcaccctt gacgatttgg atgaagccat ggattgggct gttcagaatg ggtacattac
     5581 tgcaaaccaa aggcaaaatg tccacaacag cttaatatcg atgataaaca gtgaacccta
     5641 caaacagtgg ttcagcagca cctgggaggt gaaaaacgag gcatcgatag taaacaccga
     5701 tggcaatatt taccgccccg accgagttct tattggcgat aaccaagctg taattatcga
     5761 ttttaaattt ggattagcaa ccccaaaaca ccgcaaacag gttgagcagt acaaagaact
     5821 actactaagc atgggctacg aaagggtgaa gggatattta tggtatttcc aattaaaccg
     5881 acacgaggag gtataagtca aaaatccggt aattatgaat aactttacca atcaattagc
     5941 taaatactaa ccaaaaccag tttaacaatg cgaaagtatt taatctattt aaccataccc
     6001 gctatggttg catgtggctc aaaggagcca aaaccgacca cacttgctgg tcgatacaca
     6061 aatggtaccg acaacaaggt aattgttatt agccccgatg ctattgatac catatcccta
     6121 aatgaaaatg ggctttttgt tcataaggta aacctggcca atcctgcgat gctcaaagtt
     6181 agatcgggcc gaatacaaac aaccctttac cttgaacccg gcgatagcag ttactttgag
     6241 gttgacgcca acaatgccga tggcattagc ttcagtggaa gcaacagcga actgaaccaa
     6301 aacctacgct cacgcagcaa ggaacttcaa aatattttcc gcggttggcg cgaccttttc
     6361 tcgctaaacc tcaacgaatt tggattaaag attgattcca taagcaagga attattccag
     6421 aaaaccgatt cgttaaaggc caaaagcaaa aactttgctg agatggaggc caaccgggtt
     6481 cgctacttca tgctacgctt acgtagccaa taccccgaat actcggccta cctatcgggc
     6541 gaagaattta accccgacag cgccgattac tcattctttg acaatgtgga catgaacaac
     6601 ggcgaacacc tgagttacga cgagtacgct tcgcttgttg aaacatacgc acagctcaga
     6661 attcaaaagg ctcagaactt caaggaacta tcgtcgaaac ccgcacaaga acgtcttcct
     6721 gctattttca gcatgatcga cagcattttc accaatccta aaatcaggga ttacgttaag
     6781 aaacagtacc taatggaaga gattcagttt ggtgagttct ggaagctgaa cgatatttgc
     6841 aacaactacc ttgctgcctg taccacacct gtttacaaac aggaggttca atcggtttac
     6901 aacgaaaaac taaaaattgc accgggcaac agcgccccca cttttaccta caacgatata
     6961 aacggcaagt cagtgtcact ttccgacttc aatggcaaac tggtttacat tgacttctgg
     7021 gcaacctggt gcggcccatg ccgcggcgag attcctcacc tggacaagct tgagaaagac
     7081 tacaagggga aaaagatagt ttttgtgaag atatcgctcg acgacgacat ggatgcctgg
     7141 cgtaaaatgg taaccgaaaa gaaattgggt ggcgttcagc tacatgccga tggtgcctgg
     7201 agttccgatg ccgccaagaa ttaccagatt aaaggaatcc caacctttgt gttaattggt
     7261 gccaatggta aaattatcaa ccccaatgca ccccgtccat cgtcggagga aatccgtacc
     7321 ctgctcgacc aggaacttgc aaagatttaa tgatcacacc acataaaccg aaggccggca
     7381 ttgtcggcct ttttttgttt ggcaaggttt ttctatcttt atcaggttat ttgctataaa
     7441 catgactaaa gtttacccct ggggcgacac tcgccgatac aatagttact cgggctactt
     7501 taagagggag tttgggggca gggttcaaaa gttaaccatt gatgcaggct tcacctgccc
     7561 aaaccgcgat ggcactgttg gcctgggagg ctgcacctac tgcaacaacg atgcctttaa
     7621 ccccagctac tgcacaccac aaaaatcgat tacccagcaa attgaggagg ggattgagtt
     7681 ccatgcagtg cgttacagga gggccgaccg atacctggca tacttccagg cctactcaaa
     7741 cacctatgcc ccattggaga agttaagggc gctttacagc gaagcgcttt cgcaccctaa
     7801 gattattggg ctagtgattg gcactcgccc cgactgtgtg gatgaagcta aacttgacta
     7861 ctttcagcag ctcaacagca aatactacat agcccttgaa tacggagtgg aaagctgcta
     7921 caacaggacc ttgcaactta ttaaccgcgg ccataccttt gaacaatcgg tttgggctat
     7981 taacgaaaca agtaaacgtg gaatacgcgt aggagcccac ttcatctttg gcctacccgg
     8041 cgaaacccag gagcaaatgc ttgccgaagc cgatattatt tcaaagttac ccttacacac
     8101 cgtaaaattt catcagctgc aaataatcaa gggaacaatt atggagcagc agtacacaac
     8161 caaccctgaa tggttcaagc tgttcagcat ggacgaatac tttaactttc ttgccgattt
     8221 tattgagcgg ttaaacccta actttgttat tgaacgcttt actggcgaag cgccaccccg
     8281 ctacctggca gttccaccct ggggcaacct gagaaccgac cagctgatga ttgcatttga
     8341 aaaggtgctc gaaaaacgcg atacctggca agggcgattg tttgagagca aagtttagaa
     8401 ttaaaaataa gaagctattt tagtgtctaa caattatttt aataccagtt actaaatccg
     8461 ataagaactt aaacacacaa aatatgaaac gtgtaagcat tttaattgcc ataacccttg
     8521 cagcctttac ggcagcaatt gctcagccca aggcgttaaa aaacttcgat gagcttatgg
     8581 cagccctgaa tacaggctac aatgtaaggg ttgtaataca ttacgccaag tgcaccctgg
     8641 ttgctgacaa tgaattacag gacaaatcgc ccgatgccat tgggggcatg accattgaca
     8701 cctacgagta ctttgccgct aaggccgtac gtggaaacga gcacgccttt gttgttttct
     8761 cagaaaacaa gctgattcaa aacccaaagg gaaaaggatt tgtttacaac tacgctaagt
     8821 ttaaggtcga agatactgga aaggttaagg ttacagttat gtaccttgac cccaacaccc
     8881 aagaggagtt gatgagcgaa aactttttta cacaatttgg cgacagcact aaagagcaag
     8941 caatttactt ttaccgacta taaccaaaac aatgaaacca tttcttgaga ctgttgcaga
     9001 gcatttatac tcaacacacg gaaatgatat ttcaaaatta accctggttt ttccaaaccg
     9061 aagggcatcg cttttcttca gcaagtacct tagcaagcaa attgctaaac ctatttggtt
     9121 gcctgaaaca acaaccatta gcgacctgat gttcagctat tctggtgtaa aaccagccga
     9181 tccgctaatg ctcaactata agctttacaa ggcttattgc caggccaccc acagccttga
     9241 accttacgat gagttttact tctggggaaa tgtaatgctt tccgatttcg accagattga
     9301 caaatacctg gttgacccca aaaagctatt caccaatatc aacgacctta aggaaattga
     9361 gcaacaattc aacgagtttg ataaggaaac tattgaatta attgaaaaat tcctaaacct
     9421 tgtttcatct gaaggcgaat cgctaattcg ttcacgctac tcaaaaattt gggcaaatct
     9481 ttacggaatt tacagtgcct ttaccaacag cttaatatcg gaaggaatag cttacgaggg
     9541 attagcttac cggctagccg taaaggcatt ggagggcggc acatcagcca taccaaacca
     9601 aaaatttgtt tttattgggt ttaatgcact aagcaagtcg gaacaacgat tgcttttgct
     9661 tctaaaaagt ggagaaagag cgcttttcta ttgggactac gacaattact acataaactc
     9721 cccaactggt gtcgatcacg aggcaggact attcatgcgc cagaatctta agctgtttcc
     9781 gaatgctctt aacaacacaa attttgattt cattgggaat ataggctcta gcaaaaaggt
     9841 aacacttatc tcatcgccat cggaagtaac acaaaccaaa attgttcccg atattcttga
     9901 tgagctaaac attaacaaca ctaatgagct aaccacggca gtggttttac ccgacgagaa
     9961 acttttactt ccgctgctca cggcattacc cgaaaaatac agtaatgcca atattaccat
    10021 ggagtatccg ctgcgcgaaa cttcggccta ctcttttatt gatgccctgc taactctcca
    10081 aaaatcgagc gataaacaaa ccaagtttta ctaccgcgat gttctgctaa tattatcgca
    10141 tccctacatt caacttgcat gccccgacga ggccaatagg ataagggata taatcatcga
    10201 taggcaattg ctaaacatcc cccccgacac ctttagtgga agcgacatcc tggagcaaat
    10261 tttcaagcct gtaaccaagg gttcagagct ggcaaactac cttgccacgt gcattaactc
    10321 aattattagc cgattaaccc atgaaattga tgctaacgac gataagctta agtttgaagt
    10381 tgaatacctg cttaccatta atcgagcctt aaaacggtta ggcctagtga tatcggagat
    10441 aaatgaagaa tactccaaca aaacattccg ctcataccta accaaagctt tagctgagca
    10501 aagagtatcg tttataggcg agccactttc gggtatacag ataatgggtt tcctggaaac
    10561 ccgtaacctc gactttaaaa accttgtaat tctttcggta aacgacaatc ttttaccggg
    10621 aagccacttt agcccatcgt tcatagtgcc taccctacgg caagcatacg gattgcccga
    10681 ctacaagcac caaaatgcgg tatacgccta ctacttttac cgcttaatgc aacgcgccga
    10741 aaatatctac cttgtataca caaacataac cgagggatta aaatcgggtg aactgagtcg
    10801 ctttgttaag cagcttcaaa tggagtcaaa cctaaacata aaagaagtaa ccgtaaactt
    10861 tgatcttggc gtaaatagcg aaagacctat tgaggttaca aaaaatgagg ccattgctaa
    10921 aagattaagt aagtattttg aaacaaccga gggtagcagg tacctctccc catcggcact
    10981 atcggaattt aagaactgct ccttaagttt ctttttcgac agagtgttag gcatatcagc
    11041 ccccgacgag ctcgatgaag agctcgatgc catgggcatt ggtagcgtat tccataaagc
    11101 tatggaacaa atctatagcg gttttgtggg taaagaagtg acaaaagacc aggtccagaa
    11161 cagacttaac cagcttggat tagttgaaga aactgttttt agggctttta aagaaattag
    11221 taagtttaac ggaaatatcg atgagctgaa cgggcggaat aggttgctgc tggaaagggt
    11281 gaactggatg gtaaggaatg ccctaagctc tgatcttaac aggacgccat ataaattgtt
    11341 ggcaatagag gaagaagttg aaacggttat accaattgaa cttaacggta aagccttaaa
    11401 cgtccgactt ggcggaaaaa ttgaccgttt ggaactcaaa aatgggaaac tgaccattgt
    11461 agattttaaa accggtaagc acgaaccagc caaaactaaa attagcaaca tcagcgattt
    11521 tgatgggaaa accgataagg atggactgtt ccagcaactt gtttacaagt tcatttatac
    11581 ccaagaaaat agtttaggca taggtgatgt tgacattctg ctttggtatg ttcgcagcac
    11641 cgatggaata ccaagcaccg aagttccgga taccgatgag ttttgggaag aatttattag
    11701 ttacctaaaa tcattgatat tcaaaatagt agacgatagc ccatcttcac agacatcaaa
    11761 catcaagctt tgcgaaaact gtagttataa tgtaatatgc aatagaaaag tgatgtaaat
    11821 caataaaatt taaagcgata aatattgaca tcatgactta ttcaaagtaa gtttgctttt
    11881 aacacaaata atttctctat gaaaaaacta accttatcaa tattaatact aattgcagcc
    11941 ctaagtataa aagcacagga ggttgccaca acccaaaata aaattaacct taattttaag
    12001 gttgacatgg taagcaggta cctttggcgc ggacttttac ttgacaacaa tccctgcatc
    12061 caacccgatt tatcattaag cgtcggcggt tttacctttg gtagttgggc ctcatatggc
    12121 tataacagca aatttcccga aattgatttt tacctaatct atgaatttgg cccactttcg
    12181 ttaactgttt tcgattacta caacgaggat gagaccgatt tatcatccat aaaatatttt
    12241 acctgggata gaaaattaac aggtcatgca cttgagggaa ccatcgcatg gaacggcact
    12301 gagaattttc cgcttagagc gctagcagcc acattctttt acggaaacga ccgtgatgct
    12361 gatggcaatc agtattactc cacctatttt gaaattggct atccctttaa cttgagcgac
    12421 tactcattag aattttttct gggagcaaca ccagccaagg gactttataa caatagtgca
    12481 aacattgtaa atgttggatt ttcggctagc aaggacataa aacttaccga aaattttaca
    12541 ttacctataa aaggttcctt tgtagttaat ccagcacaag aaaatgtgtt ctttgttgtt
    12601 ggtataacat tgcagtaatt ttataaccaa aacttaattc aagaggcacc caaaccggtg
    12661 cctcttatgc tacttgttat caacctatta tctataaaac ccttaggacc ttttcaagat
    12721 ccagcctaat ccgtggcgac aattctcttt gtttattctg agggttaaga ttttcgatgc
    12781 ttccgggttt tcctaaagca attaccgctc caatttggta gccatcatcg agtttaatag
    12841 cacgtctaat gcttatatgg tcaaaaccgc ccatcatatg tgtaacataa cccatggatt
    12901 gagcctgaac taaaaacatg gctgtagcca tacccaaatc gtgcagggca tagtagttgg
    12961 actcacctgt tctggtgtag tttttacggg ctgcagtaac cactaatgca cctgcactct
    13021 ttgcccactc ctggttggca ggagccaaag cgctaagaac attttcaaag gctgtggcat
    13081 tttccctgtt tgcaacataa aacaaccagg gctgttcgtt atatgccgat ggagctagta
    13141 tggcagccct aaaaagctga attaaatgct catttggaat tggctcaggg ctaaaggcca
    13201 tagcactccg gcgctgctga attgattgta atatttcgtt cattctattt ttgaattatt
    13261 tgttgcatta acaagataac gccaacaaag tttaaaaagc cattacaacg tataggtttt
    13321 ttaccctgaa ttcaatcgct aaaatcatta cgaattgtta tgaatatcat tgttttcaaa
    13381 ggttttccga cttatgtttc ataacatatt ttgtaaatct aaacaactct ttgaaaattg
    13441 gtttacaata cttaaccttg caaaacgaca aataatagat tagaagcaac ataatcattt
    13501 aaatatcaac atataacaaa aaaacaaaag ctatgagcaa gatgacacca aaggattaca
    13561 tggagcgcga aagcaagtat ggcgctcata actatcaccc actgcctgtt gtgctggaaa
    13621 ggggtcaagg cgtttactta tgggatgttg aagggaagcg ttactacgac tttctatcgg
    13681 catactcagc agttaaccag gggcactgcc acccaaaaat cattaaagcc atgtgcgagc
    13741 aagcacaaaa gttaacccta acatcacgcg cgttttataa caacgtactt ggcgaatttg
    13801 aggagtatgt aaccaaatac tttggttacg acaaggtttt acccatgaac accggagccg
    13861 aagctgttga gactgcacta aagctttgcc gccgctgggc atataacgtt aagggaatta
    13921 aggatggtga ggccaagatt attgtttgcg agaacaattt ccacggccgt acaatcaccg
    13981 taatttccat gtcaaccgac ccagattcct atggcgggtt cggtccatac actcctggtt
    14041 ttatcaagat tccctacaac gatattccag cccttgaaaa agcattgcag gaccctaatg
    14101 ttgctggttt ccttgttgaa ccaatccagg gcgaagcagg tgtgtttgtt cccgatgaag
    14161 gttacctgaa aaaagcttat gacctctgca agaagcacaa cgtgcttttc attgccgacg
    14221 aggttcaaac cggtattgcc cgcacaggta aaatgctagc ctgcgaccac gaaggtgttc
    14281 gtcccgatat cctaattctg ggtaaagcta tttcaggcgg cgcaatgcct gtttcgtgcg
    14341 tgcttgccga tgatgagatt atgcttacca ttaagccagg cgagcatggc tctacctttg
    14401 gcgggaaccc acttgccgga aaggttgcca ttgcagccct tgaggtagtt aaggaagaga
    14461 aacttgctga aaacgctgaa cgccttggca aaatcttccg cgatgagatg aagagcatca
    14521 agagcgaaat gattgagctt gttcgtggca agggcttact taacgcagtg gttatccgta
    14581 ataagcccgg caaaacagct tgggacgttt gcttggccat ggccgaaaaa ggtgttctgg
    14641 caaagccaac tcacggaaat attatccgtt tcgccccacc ccttgttatt actgaggagc
    14701 aactacgtga ggctattggc cttatcaagg aagcttttaa acaatttgaa tagtaaaaag
    14761 ttacacacca aaaaggtgac acgtacgtgt cacctttttt attttctacc ttaagtaacc
    14821 tgttcccctt tccttaacca ctctgataaa attgtcaatg gacctatcgt atccacgctc
    14881 aatatcggct ggaaaatagc aggcaggcaa ttcgccattc ttacggtgat aggctaaaca
    14941 atcgcaacac acaccatgct taccgcaggg gtaggagcag ttgcaaaact tggagttatt
    15001 ctccttttta cattccatag ctaattcact tttaaacatc ggcaaaaata attctatagt
    15061 aaaaaaactt gataaacttt cagaatgaat tgcaatctaa cctatgaatt tgcatttttg
    15121 taactacatt gttaaactca aaccaactca ccatgagaaa aatatttaca ctactaacca
    15181 ttgcaaccgc attgcttatg gcacagtgca aacaacctga acaaaaggtg gacgtatcgc
    15241 ccattagcga tgccacagtg aaaaaagtaa ctgctgaact ggttaaacag cacggcgatt
    15301 cccaaaagga aagaattgaa cgcggagttg ctcaagtggc catgctttgg aggattaccg
    15361 atggcaccga gaccgatttt gagcagttct gcaccacaca atttgtatcg gacgatgcag
    15421 cgctggagca gctattcaac aagctctcct atggttttga aatgctaaat ggctacttca
    15481 tcctgttgag taaggagctc atgaaacccc tccacttgga ctggggccca gtaaccccca
    15541 ttgatgagct ctttggctca tacaatgcgg cagcacacct aaacgacgac ctctatgcca
    15601 acaaaattgc atttattact gccctaaact tcccatacta caccctgaag gaaaaaaccg
    15661 aaatgggtga aaactggagc cgcaagcaat gggcttacgc tcgcatgggc gatatgttca
    15721 caagtcgagt tcctgcccag ctcatccaga acttttcaac agtaaacacc aatgccgaca
    15781 cctacatcag cgagtataac atttacatgg gcaaccttgt ggacgacaag ggtcaaacca
    15841 tgttccctgc cgatatgaag ttgatatcgc actggggatt gcgcgatgag ttaaagtcgc
    15901 actacgccaa caaggaggat ggacttgctc accagcagat gatttaccag gtgatgaagc
    15961 gaatcataga ccaatccata ccccagcagg ttatcaacag caacgagtat aagtggaatc
    16021 catataccaa tactgtaacc aaggaaggta atgaggttca agctacaccc gagcctaata
    16081 ctcgctacca gcatgtaata aaccaattca aggcactaaa agcaattgac gagtataccc
    16141 ctaaaatgcc aacctacata caacgcaagt ttgagggtga gtttgaacta ccggtcgatg
    16201 aggttgaaca gctgtttatt gatttggtat cgtcaaagga ggttcgcgaa gctgggaaac
    16261 taatcagcca gcgcttaggt cgcccgttac agccattcga tatctggtac gatggattta
    16321 aagcccgcag tggcatccct gctgaaaaac ttgatggcat taccaccaag cgttttcgcg
    16381 atgccaaagc ctttgaggct gagcttccaa atatcctaac ctcgctcgga tggagcaagg
    16441 aaaaagctaa attcattgcc gaccgggtaa aagttgaggc ctcgcgtggc gcagggcatg
    16501 catggggtgc cgaaatgcac aacgatgtgg cactcctccg cacccgtgtt cctgcttcgg
    16561 gcatgaacta caagggattt aacattgcca tgcatgagtt tggccacaac gtggagcaaa
    16621 ccattaccct gcacgatgtt gattactaca taatgcacgg tgtgcccaac acagccttta
    16681 ctgaggcatt ggcattcgtg ttccagaaac gcgaccttga tgtgctgggc atgaaggagg
    16741 ttaaccccga taaggaatac cttgaaaccc tcgataacct ttggtcgtgc tacgaaatta
    16801 tgggcgtatc gctggttgat atcaacctct ggaagtggtt gtacaacaac cccaatgcta
    16861 ccgccgatga ggttcgtaat gctaccctta ccattgcgcg cgacatttgg aataaatact
    16921 acgccgatgt gtttggcagc accgacgagc ctatacttgc catttactca catatgattg
    16981 acaatccgct ttacctatcg gcttacccaa taggtcatct tatcgatttt cagttggaaa
    17041 agcagttccg tggccgctca tttgctgcag aggttgaacg gatttactca cagggtcgtt
    17101 taatccctca actctggctt aagaaaggtg ttggtgagcc actttcggtt actcctattc
    17161 ttgaggcatc ggcagtagcc gtacaaaaac tctccgagaa aaagtaaatc agtccaacaa
    17221 tacttgaaag ccgtccgcat taggacggct ttttatttat accagaacag ggttagtggc
    17281 tgttttcaaa tctttttacg gtctgagcga tagttccata cttcctgccg aacaaaacca
    17341 ccgcaaacag acaaagcaat cctgctattg caagggttag ctgaacacca attgtagggc
    17401 taattgagcc aagtaaaagg cttccaagag gtgtaatacc cataaacgat aaaccgtaaa
    17461 gtgcaaccac ccttccccgc ttatcctcat caacaatgtg ctgcagaaga gtgttcgttg
    17521 ccgcaaactg aacaatcata ccaaatccag taataaatat tactccaagc gatacagctg
    17581 tatttgatga aagcgagaaa acaaacaatc caataccaaa aaggatggaa gaaacaagaa
    17641 taatctttgg taaacttcta atgcttttcc tcgaagctaa atagaatgca cccaccaatg
    17701 caccagcacc tagcgagcca gtgagaaatc caagcaactg tgagttgcca tgaaggatat
    17761 cgcgggcaaa aacgggcata aacacctgaa acggcaagcc aataaaccct attgacgaaa
    17821 ttagtaaaag taaataccta attgttttat tccggtatga ataagctaaa ccctcagcca
    17881 gttcctggaa gatggaacca tggtgatgat gagtattcat aggctttacc ctcatcgaca
    17941 ttaacgatgc tataactgcc atgaagctta acccattgat tataaagcac cacccttcgc
    18001 ctaataacgc aataataaaa ccacctatgg taggcccaat aaaacgggcc gagttaataa
    18061 gtgttgagtt taacgcaatg gcattctgaa gcaagtccct gtcaccaacc atttccagca
    18121 gaaaagcgtg acggaatgga gtatcgaaag caagcgaaac tccatttata acagcaatca
    18181 gtatgatatg ccaaatttga agcactccag taaggtaaag cgctgctagg gtaaaggcca
    18241 taaccattga taaagcctgc gaaccaataa gcacatacct tcggtttacc ctgtcggcgt
    18301 aaacgccggc tattggcgtt agaaaaagcg atgggatttg acctgcaaaa cctactaacc
    18361 caagcagaaa aggggaatcg gttaaccgat atataagcca acctaatgcc aagttctgaa
    18421 tgtaggtacc catgagcgaa acaatctgcc cataaaagaa catcctgaag ttagggcttt
    18481 tcaaagccct aaaagcaaaa ccaacccggt taaagtatga acgtatatcg atgtacctga
    18541 acatgcatta aaattgaatg cccgcggcta aacccaccat tccattccca ttaaagtagg
    18601 gaacaattac agcatgcatt tgacccctac tccgttgttt tagtttaaat cgttcgtaag
    18661 tatagtacga tattttagta cttagtattc caattcctgc acctgcaaaa acatcggtta
    18721 tccaatgctt gttattgatt acccttgcaa aaccaattgt acctgcaatg gcataaccag
    18781 ccacaccaat ctatactgta tggtccttgt actcctgcca caaaaactca gctcctgtaa
    18841 aggccatagc agtatgtccc gatgggaatg aggttgtaga actactatcg ggccgcataa
    18901 caccggttgt atgcttaagt actcgcacca ctgccaattg cagtacaaac gagccagaaa
    18961 gtatagcagt tctgtcccaa aacgaatgct tcgcctttag tcccgcatcg tcgagtatgt
    19021 aaaccgctaa gccaggaaca tactgaaagt aatcgtcggc tctgtacttt gtatttatgt
    19081 gctctaacac ctcgctgcgg attccagtgt tccagcgttt cagtttcgga ttattgagac
    19141 caaccacacc ataggtgatt agcgatactg gaataaatgt attcttaaca gtgagcctat
    19201 actccagctt tggccgttga cttaggctat caatccgagt tagctgttgt gaaaagccat
    19261 ttaggtaaaa aagtatccct aaaatcaaaa aaaatcttgt tttcaaaacc tctctttttt
    19321 tttgcaaaga taaacatcta aatatgacta tt
//
LOCUS       DAOSAL010000050        19080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_2017_length_19080_cov_48.162523, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000050 DAOSAL010000000
VERSION     DAOSAL010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 19080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19080
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2017_length_19080_cov_48.162523"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      complement(<1..88)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(157..459)
                     /locus_tag="PK049_07800"
     CDS             complement(157..459)
                     /locus_tag="PK049_07800"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HOK61500.1"
                     /translation="MRKIITLTLILLGFAVLTSQAQKVEIKSVTFEVNMHCQSCKAKI
                     ERDIVFEKGVKEVEATLDKKLVTIKYDATKTDPEKIAKAIEKLGYTAKVVETKQKG"
     gene            complement(483..692)
                     /locus_tag="PK049_07805"
     CDS             complement(483..692)
                     /locus_tag="PK049_07805"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HOK61501.1"
                     /translation="MMTIKLKVKNMKCMGCVNTILGNLPTVNGITNVKADLVSQTVTF
                     DYETQTSLEQAIAKLKELGYPAEKQ"
     gene            complement(716..2920)
                     /locus_tag="PK049_07810"
     CDS             complement(716..2920)
                     /locus_tag="PK049_07810"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOK61502.1"
                     /translation="MKRILTLLLLWFSAQHIIKAQSKDFVYGYVYELKEKGDSIPLQG
                     AAVIWLNSQVGTTTDESGYFSLNKVQGNRIAISFVGFETDTISIENVDFPLRHVLKST
                     ISIDAVTVKADRTNTFIQQLNPIQTVVLTSGELTRAACCNLSESFETNASVDVSYSDA
                     ITGAKQIQLLGLAGIYTQMQYENIPTMRGLASTFGLSYVPGPWMESIQVSKGTASVSN
                     GYESITGQINIEYKKPWAEEKSYLNLYTNSLGMAELNANYAFDVTPTVSSMVLVHGSM
                     LSNRVDHNHDQFLDHPLTQQYHLFNRWKYQGKKWESQVGLKFMNEDRLAGQVKFYTNT
                     QPLIGNPYGVKYSTQLLEAFNKTGYISQRPNTSIGLINAVSLHSQEATIGVRMHNAKQ
                     LSYYGNLVAITYIGNTNHNIKGGATLRYDRYDETFNNTPYNRTEWVKGIYGEYTYKHL
                     EMLTLMAGIRVDQNSRFGTLVTPRFHGMVKPVSTLTIRFSAGKGYRSPNFIAENSNLL
                     VSSRNLVVEENPRIEEAWNAGISMNQKFKFLDQNYSLTADYYYVWFENQFIADIDRDN
                     SAVYFYNLKGKSYSNTLQIELSGEPIRNLQFTLAYRINDVKQTINDTLKQKPLVSRNK
                     GLFTAGYKTPSRKWQFDYTIQYNGSGRLPKGYGLNLETDYKPFITMNVQVTKLFRKFD
                     FYIGVENLTGFTQKDPIVSWENPWSSTFDASQIWGPLTGQKFYAGFRMSIWK"
     gene            complement(3021..3482)
                     /locus_tag="PK049_07815"
     CDS             complement(3021..3482)
                     /locus_tag="PK049_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61503.1"
                     /translation="MKKVTQKIVSITIATIVLLSTSGFNLFIHHCQCEGEIHLSIFNE
                     VGCNHIHSKTHAHCCIVHFDETLKFDEKQSCGCWNEVLKVKTDSYSNIQKVETGNLFS
                     KYIAVNHHKGLNTTIHKLIPTYKTNWDKAPPCSTGKKICIENHQLKIAHTI"
     gene            complement(3698..6199)
                     /gene="feoB"
                     /locus_tag="PK049_07820"
     CDS             complement(3698..6199)
                     /gene="feoB"
                     /locus_tag="PK049_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOK61504.1"
                     /translation="MLLSELQNGEYGIIAKVKGRGAFRKRITEMGFVKGKKVTVVKNA
                     PLKDPIEYSIMGYEVSLRRSEASLIEVITPSEAKQILNYHPDNHQLVTHTEPEIIDTG
                     VKELSKTINIALVGNPNAGKTSIFNHLAGAREHVGNYSGVTVDSKNAKFKYKGYTINI
                     TDLPGTYSLTAYTPEEVFVRRFIHEQNPDIVINVIDSSNLERNLYLTTQLIDMDIRVI
                     VALNMYDELLEKGDRFDYTMMGKLLGIPFVPTVGSKGKGLDRLLDTTINVYAEKDKVT
                     RHIHVNYGEEIEKSILSIQNEIKKNTAITINYSSRYLAIKLLEKDKVADKVLRNEPNY
                     QEIRNQTDREVSRLESLFHEDTETLITDAKYGFIAGALKETFKESSHKKRLVTEQIDA
                     ILTHRLLGFPIFIFLIWLTFYTTFTLGQYPMEWIELGVEWISNTISTYMASGPLKDLL
                     VNGIISGVGSVIVFLPNILILFLFISLLEDTGYMARAAFIMDKLMHKIGLHGKSFIPL
                     VMGFGCNVPAIMASRTIENRNNRLVTMLITPFMSCSARLPVYILILGAFFPENAGNLL
                     FVIYFTGILLAIGTALAFKKTIFKAEDLPFVMELPPYRMPTLRSTLLHMWHKGSQYLK
                     KMGGVILVAVIFVWALGYYPRKVNYTRDYNAEIETITQKISEEKLSQPSMDSLNQTIH
                     QLELEMEAERQANSYLGRIGKFIEPAIRPLGFDWKMGVSLLSGVAAKEIVVSTLGVLY
                     QTDPDSDSGGLAQKLRNETHQSGKLSGQVVFTKVTALAFLIFILVYFPCVAVISAIKN
                     ESGSWKWALFTIVFTTSLAWVLAFLVYQIGNIIIG"
     gene            complement(6361..6732)
                     /locus_tag="PK049_07825"
     CDS             complement(6361..6732)
                     /locus_tag="PK049_07825"
                     /inference="COORDINATES: protein motif:HMM:NF023109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HOK61505.1"
                     /translation="MIEEFDNTNDLDKNTREEVFSQVVKAGKRTYFFDVKATRGNEYY
                     LTITESKKRMGKDGKMYYEKHKIFLYKEDFEKFIESLNSVFDYIKANQEIVPRTDHTD
                     EMELSEVESDYSSVDFDDLGK"
     gene            6932..7930
                     /gene="nusB"
                     /locus_tag="PK049_07830"
     CDS             6932..7930
                     /gene="nusB"
                     /locus_tag="PK049_07830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOK61506.1"
                     /translation="MINRRLIRIKALQVLFAFFRNEGDSLSALERELFHSIEKSYHLY
                     LLLLLLPENMVEHAQAKIELGKQKFRPSPEELNPNLRFVQNRAVAALAACKELQAKAN
                     DNRLNWKHFPEVTKSLFNHFVASDFFSDYMNAEHNSFADDKAVLVNLLSKILNANDDF
                     EAFLEEQSIYWNDDLEFVSSMASKTIKKIDENTASVPVMSLFSNADDEEFAKRLLRKT
                     VLRHKENLELIDKFTVNWELDRIAAMDIAIMEMAITEILEFPTIPVKVSMNEYIDIAK
                     FYSTEQSNSFVNGVLDKVIAHLKENGQIQKQGRGLIGENDESLSPENINSFEDEDN"
     gene            7917..8330
                     /locus_tag="PK049_07835"
     CDS             7917..8330
                     /locus_tag="PK049_07835"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOK61507.1"
                     /translation="MRITSLLFGFTALFAISCGSNNNQGSTQETDSTQLAELTYVEDF
                     FDVGTIHSGEIVRHSFRFRNTGNAPLVVKDIIPSCGCTKVDVTSRILKPGEEASVEVV
                     FDSKGWFGSQYKSVTLRTNGVIREKSVTLKANVVP"
     gene            8457..8717
                     /gene="yajC"
                     /locus_tag="PK049_07840"
     CDS             8457..8717
                     /gene="yajC"
                     /locus_tag="PK049_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOK61508.1"
                     /translation="MTFLMFGLIIVVFYFFMIRPQMKRQKELRKFQESLKKGDKVIIA
                     GGVYGKVTEVKDEYVLVEIADNVEVKVAKSTIIRDVTDMQTR"
     gene            8871..9860
                     /locus_tag="PK049_07845"
     CDS             8871..9860
                     /locus_tag="PK049_07845"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HOK61509.1"
                     /translation="MNQQSIKKYNQFFQPDKIRLNKRVYMFLFFLVISIILWLLIKLR
                     YEYVSTITYKVNLSELPETKVALTSDSLTVTLRVKALGYNLLRYKLFKYYKPLSVSAQ
                     GADFVKGNCYVVLSGQLDNFTSQLGTDFTLLEVSPDTLYFHLSDVVQKRVPVKPDLNI
                     TYSKQHMQAGSYTVVPKVVKVTGPASIIDTLSCVYTQPLTLEDIYDNINTTIPIKPIK
                     GLSFKPSEVSISIPVEKFTEARVNVPISCINTPYGYEVMLSPKTVSVTCNVAVSKYFA
                     LKPDHFSIICDYNDLVMESSGKAIVKLVRYPDFIGNIQIDPRKVDFVIVKKKQ"
     gene            9860..10456
                     /gene="coaE"
                     /locus_tag="PK049_07850"
     CDS             9860..10456
                     /gene="coaE"
                     /locus_tag="PK049_07850"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOK61510.1"
                     /translation="MLLRVGVTGGIGSGKTLVCKILEVLGYPVFYSDLVAKSITDTDP
                     EVMEKVKGLFGNNIYPNGKLNRKKVAELVFANDTLLKELNSIIHPAVARSFEQWCLQN
                     AKSGLVFKESAILFETGIFRELHKTILVTAPEELRIKRVVERDGVTELEVRARMAKQM
                     PEEQKKKLADFIVDNSSNQLVLPQVLGIVQILTSIKNG"
     gene            10449..11165
                     /locus_tag="PK049_07855"
     CDS             10449..11165
                     /locus_tag="PK049_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HOK61511.1"
                     /translation="MGKYGKWITGGLGWALFGPIGAILGFAIGSIFDSMETTKIYTGE
                     TSRNGFIVSLLVLVSAVMKADGKVLKSELEYVKSYFYTNFGPAAAREAILLLRDILKQ
                     AVPLRDVCLQIKENVDYHSRLQLLHFLFGIAQADGVVSSEELKVIIEIAGYLDISDAD
                     FSSIKAMFIPDTDKYFKILEVSPDATNEEIKKAYRQMAIKYHPDKVQHLGEDIRQAAE
                     QKFKMVNEAYEKIKKERGFN"
     gene            11178..11474
                     /locus_tag="PK049_07860"
     CDS             11178..11474
                     /locus_tag="PK049_07860"
                     /inference="COORDINATES: protein motif:HMM:NF016109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQ-loop repeat-containing protein"
                     /protein_id="HOK61512.1"
                     /translation="MNTADFIEAVGWFGNIILSIGVLPQVVQTWRTHDVQSFNWLFLL
                     MWAFGVLFTFVYIAYNDMVVGKYQWPLWLNYLVNIIATFYLVWAKYYYGRLKKS"
     gene            11525..11956
                     /locus_tag="PK049_07865"
     CDS             11525..11956
                     /locus_tag="PK049_07865"
                     /inference="COORDINATES: protein motif:HMM:NF037329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HOK61513.1"
                     /translation="MEVKLKELLAISGYSGLFRFISQGRQGVIVESLTDGKRTLIPAS
                     ARVSAIADIAVFTQTEEIPLKDVLKKIEVLENGGPAINNKSADKEIRSYFEKVLPEFD
                     RDRVYTSDIKKIIGWYNLLQSKELLEVLNQTDEGVKPEGEK"
     gene            complement(12006..13979)
                     /locus_tag="PK049_07870"
     CDS             complement(12006..13979)
                     /locus_tag="PK049_07870"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOK61514.1"
                     /translation="MRFLAFIAILLAYSTITAQEKEIDLYSLSLEDLINIEVSISTKT
                     KVGLRETPGIVTIITKEEIKRSGARDIIELFHLFVPGFDFGVDVEGVVGMGIRGIWAH
                     EGKYLFMVNGFTMNDGMFACVPFGNHFTLDNVEKIEIIRGPGSAIYGGFAGLGVVNII
                     TRDTYELGGKVTYTATHTGKQFSHNQLSVGQTIHDKDLLMNISATYGAGSRSDRAYTD
                     YYRNSRDLLKASDTYTKQLALKLDYKGFTIQTFLDDYLYYQIDLWDELYRGTPVHESF
                     KSNFVDLSYKFQRANLNVIPHITYEWQKPWQVNIPEINYSNNKIFQSLTPGISTYYSK
                     KTIQFVNGIEFKTDRLRLPSMVDTLYEETFKNGKDLLSYTNFSAYSQLLWNSKYVNLN
                     LGARYDFSSEFGDALTPRIGLTKLFKRYHFKLMGSKSFRTPGGILPNRIPNDFPKLYP
                     ESGITFEFESGFLISNNSMITLNLYDITFEDIIIYGKDAQSGVGYYKNQGKIGTRGLE
                     MGFKMNTSKVLSTINVAYYSRKSSDSDSLFMVPDKTNQYLALSPLRINGLISYKVSNR
                     TSFSVTASYFGERYSYNYIANNNDILVKQKTNLLVGMNMIVSDFPFKNFETQLIATNL
                     LGSDFYYLQPYKGAHGPLPGLDRSFGFRVIYEY"
     gene            complement(14018..14671)
                     /locus_tag="PK049_07875"
     CDS             complement(14018..14671)
                     /locus_tag="PK049_07875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOK61515.1"
                     /translation="MGIDLKSFNGFIFDMDGVIVDNYLYHIDAWGEFCKRHGLNFEVE
                     DFTRKYFGKNNTDILQALFGKSLSSTEIDMLGEEKEAIYRELYQPYIKPLDGLVDFMK
                     ELRIQGGKIAIASSAPQSNINFVVEKICINEYIDVSVNGNMVRLGKPNPDIFLKAAEL
                     LSIQPGKCLAFEDSFSGIQAAINAGMQVVALATTHKKEEHDPTITIISNFKELLQGY"
     gene            14796..15242
                     /locus_tag="PK049_07880"
     CDS             14796..15242
                     /locus_tag="PK049_07880"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOK61516.1"
                     /translation="MRTVFVFTALVLFSFALRAQEVSVYSVKNGKGDLITVSADTLNM
                     GEIYLDDLSSEHGVLLINLTNSGKKPLLIRNVTGCCGTNIKTWSKAPVLPGKQSEIRV
                     EFRIEPKPQRISRTVTLESNALGKPIIKLHIVGLVMQRRQANELVL"
     gene            15309..16526
                     /locus_tag="PK049_07885"
     CDS             15309..16526
                     /locus_tag="PK049_07885"
                     /inference="COORDINATES: protein motif:HMM:NF013857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61517.1"
                     /translation="MKKRLNTTIFDLPVQELRRGYRSDVYFWREKVVLENHDLHPRVT
                     MQVFQKNNAILCGIDEAIAVLKVASGRYSDYSKAYQLFDKLIDLKKLARKQFLTDKNR
                     YLQIEEERIKVSQELDNLWIDGFSEIEIDALWDGDSISPYESVMHIHGDASLFAHLET
                     IYLGILARRTKIATNVRRVVEAANGKTVLYFPARFDHWAVQGGDGYAAHIGGASGVST
                     DAQGEWWGAGASGTIPHALIAAVGGNTVKAVQFFHDTYPNATLIALVDFDNDCVNTAL
                     ECARAMGDKLWGVRLDTSENLVDKSVFPLMGTYKPTGVNPKLVELVRSALDREGFSNV
                     KIVVSGGFNPDRIRQFEGMGVPVDAYGVGSYLMQGSYDFTADIVHVNGKPMAKVGRYF
                     MPNPRLQRYNPNE"
     gene            16532..17074
                     /locus_tag="PK049_07890"
     CDS             16532..17074
                     /locus_tag="PK049_07890"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HOK61518.1"
                     /translation="MKALIVVDVQNDFCPGGALAVTDGDKVVDPINELSKQFEADGLP
                     IIFTRDWHPANHLSFKENGGVWPPHCIAGTPGAAFHPNLYFPSVAFLVSKATEPDMEA
                     YSGFQGTGLASWLRQIDVDELVVAGLATDYCVKNTVTDAIKLGFKVTLALKAIKAVNV
                     NPTDGETAINEMKSLGVQIV"
     gene            complement(17134..>19080)
                     /locus_tag="PK049_07895"
     CDS             complement(17134..>19080)
                     /locus_tag="PK049_07895"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HOK61519.1"
                     /translation="CYHAGVSVNMNYATDGSGAFSRDVLYALTNYFNYDPTTIQIYTF
                     NPDNQTEWINMVKTEIDAGRPIYYSGSSSASGGHAWVCDGYNDNNELHINWGWGGYAN
                     GYYAATAMIPSGTSYDFSESNDMIIGIQPNQSEFKHLWVRQASGFGTSSRGIQYISAV
                     SNRVAWAVAFDGSGGNAKVKEYTMTTDGGATWKAGSINPANSAGFAASMICAIDDKTA
                     WVPLYDGTNGGGMIAKTTDGGKTWTQQTSATFTKPNGFPNVVHFWDADNGFCMGDPNG
                     GYFEIYTTTNGGTNWTRVSQANIPAPLNGEYGVVGYYDVIGDNVWFATNKGRIYKSTN
                     KGASWQVYQTPLTSTAFEIAFKNSNEGVIYGWENNIDKYYRTTDGGTTWTEFTPSGSV
                     YTADLCWIPGTDTLISTGSNYSANKMGVSYSVDGGNTFTDYAPFYKNFQFTNMGASPN
                     GTVWAGSFNSSANYGGMWKKGASVLSANFTVNKTTAYRNDSTVVFTDMTYGSPESWEW
                     NFGEGAEPATRTGKGPHTVKYTTYGDKTITLTVQKGNDIHVYVKEKVLNVTWPSGVET
                     NNSDNKLTVYPNPTNSSVFVNIAGFTKGIIDVYNITGALVWTSGTETTEGKIDVSNLS
                     TGVYLIKIKGDNGTVATRKLTVTR"
BASE COUNT         5339 a         3794 c         3892 g         6055 t
ORIGIN      
        1 aacctcaaaa cttccctacg caggtattat cctgttcagg ttgtaagggt attatctcag
       61 ccttactcag gcacccctag ttttgtgtat gtaaatgtag aaaaaacgga aaccgttgtt
      121 tggtttccgt ttaaaatttt tcaacaaaaa aagggattaa cctttttgct tggtttctac
      181 tacctttgcg gtgtagccaa gtttctcaat tgctttggca attttttcgg ggtcggtttt
      241 tgtggcatcg tacttaatgg ttactaactt cttgtcaagg gtggcttcaa cctccttaac
      301 ccctttttca aaaacaatat cgcgttcaat ttttgccttg cacgactggc agtgcatatt
      361 aacctcaaag gttacagatt taatttcaac cttttgcgct tgcgacgtta atacagcaaa
      421 tcctagcagt attagtgtca gtgtaataat ctttctcatg gtgtattagt atttatggtt
      481 acttattgtt tctcggcagg atatcccagt tctttaagtt ttgcaatagc ctgctcaagg
      541 cttgtttgcg tttcatagtc aaaggttacg gtttgtgaaa ccagatcggc tttcacgttt
      601 gtgattccgt taaccgtagg taggtttccc agtatggtgt taacacaacc catacatttc
      661 atgtttttca ctttaagttt tattgtcatc atagtagttt attttaaggt ttatactatt
      721 tccagatgga cattctaaat ccggcataaa atttttgacc tgttaagggg ccccaaattt
      781 gcgaggcgtc aaatgttgaa ctccacggat tttcccacga tacaatagga tctttctggg
      841 tgaaccctgt aaggttctca acgcctatgt agaaatcaaa cttacgaaac agtttggtta
      901 cttgtacatt catggtaatg aaaggcttat agtccgtttc aaggtttaga ccatatcctt
      961 tggggagtct tccgctaccg ttatactgaa tggtatagtc gaactgccat ttacgcgatg
     1021 gggttttgta accagcagta aacaagcctt tgttgcggct tacaagtggc ttttgcttta
     1081 acgtatcgtt aatggtttgt ttcacatcgt ttattctata tgccaaggta aactgtaggt
     1141 ttctaattgg ctctcccgag agttctatct gtagggtatt tgagtacgat ttccctttca
     1201 gattgtagaa gtaaaccgca ctgttatccc tgtcgatatc ggcaataaac tgattttcaa
     1261 accatacata gtaataatcg gcagttaaac tatagttttg atccaaaaat ttgaatttct
     1321 gattcatgct aatcccggca ttccaggctt cttcaattct tggattttcc tctactacca
     1381 ggttccgtga actcaccaat aggtttgagt tctcagcaat gaaatttggc gatctgtaac
     1441 ctttacctgc gctgaaccta atggttaagg ttgaaactgg ttttaccatt ccgtggaaac
     1501 ggggtgtcac aagagtacca aacctactat tttggtcaac ccttatgccg gccattagcg
     1561 taagcatttc cagatgttta taggtatatt cgccgtaaat acctttaacc cactcggtgc
     1621 gattgtaagg ggtgttattg aacgtttcat cgtacctatc gtacctgagg gttgcgcctc
     1681 ctttaatgtt gtggtttgta ttgccaatat aggttattgc aaccaggtta ccgtagtagc
     1741 taagctgttt ggcgttgtgc attctaacac ctatggttgc ttcctggcta tgtagtgata
     1801 ctgcattgat aagcccaata cttgtgtttg gcctttggga aatatatcca gttttgttaa
     1861 aggcctcaag taattgggtt gagtacttta ccccgtaggg gttgccaatc aggggctgcg
     1921 tattggtata aaacttaacc tgtccggcca atctgtcctc attcataaac ttcagcccaa
     1981 cttgcgattc ccacttctta ccctggtact tccatcggtt aaacaggtgg tactgctggg
     2041 taagcgggtg gtcaaggaat tggtcgtggt tgtgatcaac ccggttgctt agcatacttc
     2101 catgcaccag caccattgac gatacggtgg gggtaacatc gaatgcgtag ttagcattta
     2161 gttcagccat tccaagcgag ttggtgtaaa ggttcaggta cgacttttcc tcagcccagg
     2221 gtttcttgta ctcaatattg atttgtccgg ttatgctttc gtacccgttg ctgaccgatg
     2281 cagttccctt tgatacttga attgattcca tccatggccc cgggacgtaa ctcagtccaa
     2341 aggttgatgc caaccctcgc attgtgggta tattttcata ctgcatctgg gtgtatattc
     2401 ctgctaaccc cagtagttga atttgctttg caccggtaat ggcatcgcta tacgacacat
     2461 caacggaagc attcgtttca aagctttccg ataggttgca gcatgcagcg cgtgtaagct
     2521 ctcccgatgt aagtacaact gtttgtattg ggtttagctg ctgtataaag gtatttgtcc
     2581 tgtcagcttt taccgttacg gcatcgatac ttatagttga tttcaggaca tggcgtaacg
     2641 gaaaatctac attctcaata ctaatggtat cggtttcaaa accaacgaaa cttatagcta
     2701 tccggttgcc ttgcacctta tttaaagaaa agtaaccgct ttcatcggtc gttgtaccaa
     2761 cttggctgtt cagccaaatt acggcagctc cttggagcgg aatactgtcg cctttctctt
     2821 tcagctcgta aacataaccg tacacaaaat ctttactctg tgctttaatg atatgttgag
     2881 ctgaaaacca tagtaacagt aatgttaata tgcgtttcat aatagaaatt cttaaattgt
     2941 tgttgaaatc aaatatgagc gctacaagca aaaaatgagc gcaattaaga gctgcagaga
     3001 gaaaaaactc tctgtaacga ttaaattgta tgggctattt ttagctgatg attttcaatg
     3061 cagatttttt tccccgtact gcagggtggg gctttatccc aattggtttt gtaagttggg
     3121 attaacttat ggattgttgt atttagcccc ttgtggtggt ttactgctat gtactttgaa
     3181 aaaagatttc cggtttcaac cttctgaatg tttgagtagc tatcggtttt aacctttaac
     3241 acctcgttcc agcatccaca cgattgcttt tcgtcaaatt tcagtgtttc atcaaaatga
     3301 acaatgcagc agtgtgcatg tgttttggag tgtatgtggt tgcatccaac ttcattaaaa
     3361 atggacaggt gaatttcacc ctcgcactgg caatgatgta tgaagaggtt aaacccacta
     3421 gtggatagta gcactatagt tgctatagta attgatacta ttttttgggt aacctttttc
     3481 atcttacatc tcacctaata tagctaaaca taaaaaggaa caaaaagttc atttttttga
     3541 gggttgattc aaattgctta ttgtgcatga agccgatgaa catccggcac atttacccga
     3601 atttatacgt tttgggttaa aaatttgata cacattgtat gcagtatatc ccactgctcc
     3661 taatactatt attaatactg ctatttcctg aaccatatca accaattatt atattaccaa
     3721 tttggtaaac caaaaatgct aaaacccatg ccagcgaggt agtaaaaact attgtaaaga
     3781 gagcccactt ccacgaaccc gactcgttct taatagcaga gattacagcc acgcagggga
     3841 agtaaactag aataaagata aggaatgcga gtgcagtaac ttttgtaaaa accacctgtc
     3901 cgcttaattt ccctgattgg tgggtttcgt ttcggagttt ttgagcaagt ccaccgctgt
     3961 cggaatcggg atcggtttgg taaagtacgc ccagcgtact aactacaatc tccttggcag
     4021 caactcctga aagaagactt acacccattt tccaatcgaa cccgagaggg cgaatggctg
     4081 gttcaataaa tttgcctatc cgtccaagat acgaattggc ttgcctttcg gcctccattt
     4141 ccaactcaag ttgatgtatt gtctggttta ggctatccat agagggttgg gagagctttt
     4201 cttccgaaat cttttgcgta atagtttcaa tttcggcatt ataatccctg gtgtagttca
     4261 ctttgcgagg gtaatagccc aatgcccata caaagattac agctaccagg attactcccc
     4321 ccatcttttt taggtattgc gaccccttgt gccacatgtg caatagggtc gatcgtaaag
     4381 ttggcatacg gtagggtgga agttccatta caaagggcaa atcctccgct ttgaaaatgg
     4441 ttttcttgaa tgctagggct gttccaatgg caagcagtat acccgtaaag tatataacaa
     4501 acagaaggtt tcctgcgttt tccgggaaaa atgcacccag aataagtatg taaaccggca
     4561 atctggcgct acagctcatg aaaggcgtaa tgagcatggt tactaatcgg ttattgcggt
     4621 tttcaatggt tcgcgaggcc attatggctg gaacattgca tccaaacccc atcacaaggg
     4681 gtatgaacga tttaccatgg agcccaattt tgtgcatcag cttatccatg ataaatgcag
     4741 cacgtgccat atacccagta tcctcaagta gcgatatgaa taggaaaagg atcaagatat
     4801 tgggtagaaa aacaattaca cttcctacac cgcttataat cccgtttacc aaaaggtctt
     4861 ttaaagggcc actggccatg tatgtactaa tggtgttact aatccattcc actcccagtt
     4921 caatccactc catagggtat tgccccaggg tgaatgtggt gtaaaacgtg agccatatca
     4981 ggaaaataaa tatggggaat cccagtaaac ggtgggtaag tattgcgtcg atttgttcag
     5041 taaccagacg cttcttgtgg ctgctctcct tgaatgtttc ctttagtgca cctgctataa
     5101 agccatattt ggcatcggtt attagggttt ccgtatcctc gtggaaaagg ctttcgagcc
     5161 tgcttacctc acggtcggtt tggtttctga tttcctggta gtttggttca ttgcgcagca
     5221 ctttatctgc taccttgtct ttttccaaca gcttaatggc aaggtatcgg ctggaatagt
     5281 tgatggttat agctgtattt ttcttaatct cattttgaat ggatagtatc gatttttcaa
     5341 tctcttcacc gtagttaaca tggatgtggc gggtaacttt atccttttct gcataaacat
     5401 ttatagttgt atctaaaagc ctgtcgagcc ccttaccttt gctaccaacg gtaggtacaa
     5461 atggaatacc aagtagtttg cccatcatgg tgtaatcgaa cctgtcgccc ttctcaagta
     5521 gttcatcata catattcagg gcaacaatca ccctgatatc catatcaata agctgggtgg
     5581 ttaggtataa gtttcgctca aggtttgatg agtcaataac atttatgaca atgtcagggt
     5641 tttgctcatg gataaatcgc cgtacaaata cctcctcggg tgtataggct gtaagggagt
     5701 aggtacccgg taaatcggta atgttaattg tatatccctt atacttaaac tttgcgtttt
     5761 ttgaatcgac tgtgaccccg ctgtaatttc caacatgctc ccttgctcca gcaaggtgat
     5821 taaagatact ggtcttccca gcattgggat taccaaccaa cgcaatattg atagttttgc
     5881 taagttcctt taccccagtg tcaataattt caggttcagt gtgggtaaca agctgatggt
     5941 tatcaggatg atagtttaaa atttgcttag cctcactggg tgtaataacc tctattagcg
     6001 aagcctcgct ccgacggagc gatacctcgt aacccataat tgagtattca ataggatcct
     6061 ttaagggagc gtttttaact acagttactt ttttcccttt cacaaaaccc atttcggtaa
     6121 tacgcttcct aaaggccccg cgtcccttta cttttgctat tatgccgtat tccccgttct
     6181 gtaactccga tagtagcatc tagtttaaaa tcagtaacag taaaacctca atgtttcaaa
     6241 aaagttttgg aattactaaa attttacaaa tgtaagagtt ttctgttatt tagatcgatt
     6301 ttaagtaaaa aagccattca ttactgaatg gctgtactgt tcgaaaatcg ttataaaaaa
     6361 ctatttacct aagtcgtcaa agtcaactga tgagtaatcg gattcaacct cagaaagttc
     6421 catttcatcg gtatgatcag tacgtggaac aatttcctga ttagctttaa tgtagtcaaa
     6481 aacactattc aggctttcga taaacttttc aaaatcctct ttgtatagga agattttatg
     6541 tttttcgtag tacatcttgc catccttacc catacgtttt ttactctccg taatggtaag
     6601 gtaatactca ttgccgcgtg ttgcctttac atcaaagaaa tatgttcttt ttccggcctt
     6661 cactacctga gaaaaaacct cttctctcgt gttcttgtct aaatcgttag tgttatcaaa
     6721 ttcctcaatc attgctctta gtatttttag gttagggttt cacttttgca ctgctcaaaa
     6781 ttaaaaaaat cttcatatca tggttcattg acttaaaaaa attattaaaa aacacccaaa
     6841 aggttttgtt ttgaaatatt aaccttattt attccactgc actggattaa tctttctatc
     6901 tttgcaggag tgaaaaaaat cgttcttact aatgattaac cgtcgtttaa ttagaatcaa
     6961 ggccttacag gttctttttg ccttcttccg taacgaaggt gactctctta gcgcattaga
     7021 gcgcgagctg tttcatagca ttgaaaaatc gtaccatctt tacttgcttt tactgctttt
     7081 gccagagaat atggttgagc atgcccaggc aaaaattgag ctgggtaagc aaaagttccg
     7141 cccgtcgcct gaagagttga accccaacct gcggtttgtt cagaacagag cagttgctgc
     7201 attggctgcc tgcaaagagc tacaagccaa agcaaacgat aatcgcttga attggaaaca
     7261 ttttcctgag gtaaccaaat cgcttttcaa ccactttgtg gcatcggatt tcttttccga
     7321 ttacatgaat gctgagcata actcttttgc tgacgataaa gcagttctgg ttaatctact
     7381 cagcaagata ttaaatgcta atgacgattt tgaggcattc cttgaggagc aaagcatata
     7441 ctggaacgat gacctcgagt ttgtttccag catggcttcc aaaacaatta agaagattga
     7501 tgaaaataca gcatcggttc cagtgatgtc gctatttagc aatgccgacg acgaggagtt
     7561 tgctaaacgt ttgcttcgca aaacggtttt aaggcataag gaaaacctgg aacttattga
     7621 taaattcact gttaactggg agcttgaccg aatagccgca atggatattg caattatgga
     7681 aatggctatc actgaaattc ttgaattccc taccatccct gtaaaggtta gcatgaatga
     7741 gtatattgat atagccaagt tctatagtac agagcaaagc aactcgtttg taaatggtgt
     7801 gctcgataag gtaatcgctc acctgaagga gaatggacaa atccagaagc agggtagggg
     7861 cttgattggc gaaaacgacg aaagtctttc acccgaaaat attaattcat ttgaagatga
     7921 ggataactag cttgcttttt ggttttaccg ctttatttgc aatatcgtgt ggtagtaata
     7981 acaatcaggg ttcaactcag gagactgact caacccaact agctgagctc acctatgttg
     8041 aggatttttt tgatgtagga acaatacaca gcggtgaaat tgttaggcac tcgttccgct
     8101 tcagaaatac tggtaatgcg cctctagtag ttaaggatat aatcccatcg tgcggatgca
     8161 caaaggttga tgttaccagc agaattctta aacccggcga ggaggcatcc gttgaggtgg
     8221 tttttgatag taaaggctgg tttggttccc agtataaatc ggttactctt agaaccaatg
     8281 gggttattcg tgaaaagtca gtaaccctca aggcaaatgt agtaccctaa ttttcaaaac
     8341 attttactaa ctttgtatgt ttataaacct gaaattgtta attaattaaa gttgataaag
     8401 ttatgattac aagttttatt acacttcaag caacccagca gggtgctaac ccgttgatga
     8461 ccttcttgat gtttggtctc atcattgttg ttttttattt ttttatgatt aggcctcaaa
     8521 tgaagcgcca aaaggaactc cgtaagtttc aggagtcgct taagaaaggt gataaggtga
     8581 ttattgccgg cggtgtttac ggtaaggtta ccgaggttaa agatgagtat gttttggttg
     8641 aaattgccga taatgttgaa gtaaaggttg ccaagagtac tattattcgt gatgtgaccg
     8701 atatgcaaac tcgataaata ttttctttcc gagggattca aagttttgag agaagctttg
     8761 aatccttttt ttatttcaac ttgataaccc ctaataattt tagtacaacc ttaggttttt
     8821 ttaccaagat aatttacttt tggtttccaa aatcaattga attattccct gtgaatcaac
     8881 aatccataaa aaaatacaat caatttttcc agcccgataa aatccgcttg aacaagcgtg
     8941 tatacatgtt tcttttcttt ctggttatat ccataatcct ttggttactt attaagttaa
     9001 ggtatgagta cgtatcaacc attacctata aagtaaattt atccgagttg ccagaaacta
     9061 aggtggcttt aacatccgat agcctaacgg taacccttag agtaaaggct ttggggtata
     9121 atttgttaag gtataagctt tttaaatatt acaagccact atcagtatct gctcaaggtg
     9181 ctgactttgt gaaagggaat tgctatgttg ttctttcagg acaactggat aactttacct
     9241 cgcagctagg gaccgatttt actttacttg aagtcagccc tgatacactt tacttccacc
     9301 tgagcgatgt tgttcaaaaa agagttcccg ttaaacccga tttgaatatt acgtactcca
     9361 aacagcatat gcaggctggc agctatactg ttgttcctaa ggtagtaaag gttactgggc
     9421 ccgcctcaat catcgataca cttagctgtg tttatacaca acccctgacc ctcgaggata
     9481 tctacgataa tatcaataca accatcccaa ttaaaccgat aaagggctta agttttaagc
     9541 cttcagaggt aagtatttca attcctgtgg agaaatttac cgaggcgcgg gttaatgtgc
     9601 caatctcttg catcaatacc ccttatggat atgaggttat gctctcgcca aaaactgttt
     9661 ccgttacctg taacgtggca gttagcaagt attttgcttt aaaacccgat catttttcaa
     9721 tcatttgcga ttacaatgac cttgtaatgg aatcatctgg caaagccatt gtgaagcttg
     9781 ttaggtaccc tgattttatt ggaaatattc agatagatcc ccgtaaggtc gattttgtta
     9841 tagtaaaaaa gaaacagtaa tgttgcttag ggtcggtgtt accggtggta ttggaagtgg
     9901 taaaactctg gtttgtaaaa tccttgaggt tttgggttat cctgtatttt attccgattt
     9961 agtggctaaa tccattactg ataccgatcc cgaagttatg gaaaaggtca aagggctttt
    10021 tggtaacaac atttacccca acggcaagct aaaccgcaag aaggttgccg agctagtgtt
    10081 tgccaacgac accttgctga aagaactcaa cagtattatt catccggccg tagcacgtag
    10141 ttttgagcaa tggtgcttgc aaaacgctaa aagtgggtta gtctttaagg aatcggctat
    10201 actatttgaa accggaattt tccgtgaact tcacaaaact atacttgtaa cagcacctga
    10261 ggaactcaga attaaacgcg ttgttgagcg cgatggagtt acagaattgg aagtcagagc
    10321 gcgaatggca aaacaaatgc ccgaggagca aaagaaaaaa ctagccgatt tcatagttga
    10381 taacagctca aatcaactcg ttttaccaca ggtattaggc attgttcaaa ttttaacgtc
    10441 aattaaaaat gggtaagtac ggtaagtgga taaccggtgg gctaggatgg gcactttttg
    10501 ggccaattgg tgccattctg ggttttgcta ttggttccat attcgatagt atggaaacca
    10561 ctaaaattta tactggcgaa actagccgta atggttttat tgtaagccta cttgtgttgg
    10621 tctcggcagt tatgaaagcc gatggcaagg tgcttaaatc ggaactggag tatgttaaga
    10681 gttacttcta taccaatttt ggaccagcgg ctgctcgtga ggcaattctt ctgcttcgcg
    10741 atattcttaa gcaggctgtt ccgcttcgcg atgtctgttt acagattaag gagaatgtcg
    10801 actaccattc acgcttacag ctgttgcatt tcctttttgg aatagcgcag gccgatggtg
    10861 ttgttagctc tgaggagcta aaagtaatta ttgaaattgc tggctatctc gatatttccg
    10921 atgccgattt cagttccatt aaggcaatgt ttatacccga taccgataag tatttcaaaa
    10981 tacttgaggt ttcacccgat gctaccaacg aggaaatcaa aaaggcatac aggcaaatgg
    11041 ccataaagta tcatcccgat aaagtacaac acctaggcga agatatcagg caggctgctg
    11101 agcaaaaatt taagatggtt aatgaggctt atgagaagat taaaaaagaa cgagggttta
    11161 actaaatcta cactacagtg aatacagctg atttcattga agcggtgggc tggtttggta
    11221 atatcatact gagtataggc gtattacctc aagtggttca aacgtggcgg acacatgatg
    11281 tacaaagctt taactggctt tttctcctga tgtgggcatt tggagtacta tttacttttg
    11341 tatatattgc ctacaacgat atggttgtcg gaaagtatca atggccttta tggttaaact
    11401 atctggtaaa tattattgct acattctact tggtttgggc aaagtattac tatggtaggt
    11461 tgaaaaaaag ttagattact aaatttttta attaacttcg cattaacaaa caactcgttt
    11521 tattatggaa gtaaaactta aagaactact agcaatatct ggatattcag ggttattcag
    11581 gttcatttct cagggtcgcc aaggggtaat tgttgaatcg cttaccgacg gcaaacgtac
    11641 acttatcccc gcatcagcta gggttagtgc aattgctgac attgcagtat tcacccaaac
    11701 cgaggaaatc cccttgaaag atgttctgaa aaagattgaa gtgcttgaaa acggaggccc
    11761 agccattaat aacaaatcgg ccgataagga aattcgaagc tactttgaaa aggttttacc
    11821 tgagtttgac agggacaggg tttacacaag tgacattaaa aagattattg gttggtataa
    11881 cttgctccaa agcaaagagc ttttagaggt tctaaaccaa accgatgaag gggttaaacc
    11941 tgaaggtgaa aaataatttg aaagtaaata ttattatcgg ggaagcgact cactgagtcg
    12001 ctttcttagt attcataaat cactctaaat ccaaaactcc tgtctaggcc cggcagtggc
    12061 ccatgggccc ctttgtaggg ttgtaagtag taaaaatctg aacccaacag atttgtggca
    12121 attagttggg tttcaaaatt tttaaatggg aaatcggaaa ctatcatatt catccctacc
    12181 agtagatttg tcttttgctt aacaagtata tcgttattat tagcaatata gttgtatgaa
    12241 tacctctccc caaagtacga tgctgttact gaaaaggatg ttctgtttga aaccttgtag
    12301 cttattaacc cattaatcct taatggtgaa agcgctaaat actgatttgt tttatcgggt
    12361 accatgaata ggctgtcgga atcagagctt ttcctggaat agtaagcaac atttatggtg
    12421 ctcaatacct tactcgtatt cattttaaaa cccatttcca accctcttgt tccaatttta
    12481 ccctgatttt tgtagtatcc tactcccgat tgagcatctt ttccatatat gataatatcc
    12541 tcaaaggtga tgtcatatag gtttagggtt atcatcgaat tatttgatat taggaaacca
    12601 ctctcaaatt caaaggtaat acctgactcg ggatataatt tgggaaaatc gtttggaata
    12661 cggttgggta atatgccacc aggcgttcta aacgatttac tccccatcag cttgaagtga
    12721 taacgtttaa acaattttgt taatccaatt cgaggagtta aagcatcgcc aaattcactt
    12781 gagaaatcgt atcgagcccc taaatttagg tttacatatt ttgagttcca aagcaactgg
    12841 ctgtatgccg aaaaattggt gtatgatagc aaatcttttc cattcttgaa ggtttcttca
    12901 taaagggtat caaccatgct aggcagcctt agcctgtcag ttttaaattc aattccatta
    12961 acaaattgta tggtcttttt gctgtagtaa gtgctaatgc cgggtgtaag actttggaat
    13021 atcttattgt tgctgtagtt tatttctgga atgtttactt gccatggctt ttgccattcg
    13081 taggtaatat gaggtattac gtttaaattt gccctctgaa atttatacga taaatcaaca
    13141 aagttgcttt taaaactctc atgaactggt gtacccctat atagttcatc ccacaaatct
    13201 atttggtagt aaaggtaatc atctaaaaaa gtttgaattg tgaagccttt atagtcaagt
    13261 tttaatgcta gttgttttgt ataggtgtcg cttgctttta gtagatctct gctattccgg
    13321 tagtaatcag tgtaggctct atcgcttcgg gagcctgcac cataggtggc tgaaatattc
    13381 atcaacaaat ctttgtcatg aatggtttgg ccaacgctga gctggttatg gctaaactgt
    13441 tttccggtat gggtggcggt gtaggtcact ttgccgccaa gctcataagt atcgcgggtg
    13501 attatgttta caaccccaag tccggcaaat ccaccatata tggctgatcc tgggccacga
    13561 ataatttcga ttttttcaac attatcaagc gtaaagtgat ttccaaatgg aacgcatgca
    13621 aacattccgt cgttcatggt aaagccattt accataaaaa ggtatttgcc ttcatgcgcc
    13681 caaattccgc gtattcccat tcctactaca ccctcaacat caacaccaaa atcaaatccg
    13741 ggtacaaaaa gatgaaacaa ctcgattata tctcgggctc cacttctctt aatttcctct
    13801 ttagtaatga ttgttacaat acccggggtt tccctaagcc caactttggt tttggtggaa
    13861 attgacactt caatatttat caaatcttct aatgagaggg agtagagatc aatctccttt
    13921 tcctgcgctg ttattgtgct atatgctagt aaaattgcaa taaatgcaag aaacctcatg
    13981 tttttttctt gcaatatatg aatacatcca gtaaaagtca atatccctgg agtaattctt
    14041 tgaagttgct aattattgtt atagtaggat cgtgctcttc ctttttatgt gtagtagcta
    14101 acgctacaac ttgcataccg gcatttattg cagcttgaat tccggaaaat gaatcctcaa
    14161 atgcaagaca ttttccaggt tgaatactta aaagttctgc ggctttaagg aatatatcag
    14221 gattgggctt accaagccta accatattgc catttactga aacatcaata tattcgttta
    14281 tacatatttt ctcaacaaca aagttgatgt tgctttgtgg agcagaactg gcaatagcaa
    14341 tttttccccc ttgtattctt aattccttca tgaaatctac aagtccgtcc aggggtttga
    14401 tatagggctg gtataattcc ctgtaaattg cctctttttc ttcgccaagc atatcaattt
    14461 cagtactaga aaggcttttc ccaaataagg cttgcagaat atcggtatta ttctttccaa
    14521 agtatttcct tgtaaagtct tcaacttcaa aattcaaccc atgccgttta caaaattctc
    14581 cccaggcatc aatatggtat aggtagttat caacaataac gccatccata tcgaaaataa
    14641 acccattgaa acttttaaga tcaattccca tttttatgga tttttaagtt aagctttgca
    14701 aagttagcat tggcctaatc aatatttgtg ttgaatagaa taaagtttta ctatttttgg
    14761 cataaagttc gatatgttta ctaaaattga tgcatatgag aaccgtattt gtatttaccg
    14821 ctttggttct gttttccttt gctcttagag cacaggaagt tagcgtttat tccgttaaaa
    14881 atggtaaagg cgatttaata acagtttcag ccgatacgct taatatgggg gagatttacc
    14941 tcgacgattt atctagtgag catggtgttt tactaattaa tttaaccaat tcgggtaaga
    15001 aacccttgct cattagaaat gttacaggtt gctgcggaac aaatattaag acatggtcaa
    15061 aagcaccagt attaccaggt aagcaatcgg aaattagggt tgaatttcgt attgagccta
    15121 agcctcagcg aataagtaga acggttaccc tagagtcgaa cgcattgggc aagccaataa
    15181 ttaagctaca cattgttggc ttggtaatgc aacgcaggca ggccaatgaa cttgttctat
    15241 agaaatttgt taaatattga tgaaggggga ttgatgtcaa tccccttttt tactaaattt
    15301 agcaagatat gaaaaaacgt ttaaacacta ccatattcga tcttccggtg caggaactcc
    15361 gccggggtta ccgtagcgat gtgtatttct ggcgcgaaaa agttgttctg gaaaaccatg
    15421 accttcaccc tagggtaacc atgcaggttt ttcaaaagaa taacgctata ctttgtggta
    15481 ttgatgaggc tatagctgtt ctcaaagtgg caagtggccg atattccgat tactcaaaag
    15541 cataccaact tttcgataaa ctaatcgatt taaagaaact tgccagaaaa cagtttttaa
    15601 ccgataaaaa taggtatctg caaattgagg aggagcgtat taaagtatcg caggagttgg
    15661 ataacctatg gattgatggt ttttcggaaa tagagattga tgccctatgg gatggcgatt
    15721 ccatatcgcc ctatgaatca gttatgcata tccatggcga tgcctcgtta tttgcacatc
    15781 ttgaaactat atacttgggt atccttgcac gccgtaccaa aattgctacc aatgtcagaa
    15841 gggttgttga agcggcaaac ggtaaaacag ttttgtactt tcccgcccgg ttcgaccatt
    15901 gggctgttca gggtggcgat ggttatgctg ctcatattgg cggtgcatcg ggcgtttcca
    15961 ccgatgcaca gggcgaatgg tggggagcag gtgcttccgg aaccattcct catgccttaa
    16021 tagccgccgt tggtggcaat accgttaaag cggttcagtt ttttcatgat acatacccta
    16081 atgcaaccct tatagctttg gttgattttg ataacgattg cgtaaatacc gctttggagt
    16141 gtgcccgcgc catgggcgac aagttatggg gtgttaggct cgacacctct gagaatcttg
    16201 tcgataaaag cgtattccca ttaatgggaa cttacaaacc taccggggtt aaccccaagt
    16261 tggtagaact tgtgcgttca gcccttgacc gcgaaggatt ttcaaatgta aagattgtgg
    16321 tttcaggagg ttttaaccct gaccgtataa ggcaattcga gggaatgggc gttccggttg
    16381 atgcctatgg tgttggcagt tatctcatgc agggaagtta cgattttact gccgatattg
    16441 ttcatgttaa tggtaaaccc atggctaagg tcggtaggta ttttatgcca aacccacgtt
    16501 tgcaaaggta taacccaaac gagtgaatac tatgaaagca ttaatagtgg tcgatgtgca
    16561 aaacgacttt tgcccaggcg gtgccttggc agtaaccgat ggcgataaag tggttgaccc
    16621 cattaacgag cttagcaaac aatttgaggc cgatggatta ccaatcattt ttacccgcga
    16681 ttggcatcca gctaaccatt tgtcattcaa agagaatggt ggggtttggc ctccgcattg
    16741 cattgcagga actccaggtg ctgcttttca ccccaactta tactttccct cagttgcttt
    16801 tcttgtatct aaagccaccg agcccgatat ggaggcatat tcaggttttc agggaactgg
    16861 tttagcatcg tggttacgtc aaatagatgt tgacgagtta gttgttgccg gtttagccac
    16921 cgattattgt gtgaaaaata ctgtaactga tgccataaag cttggattta aggtaacgtt
    16981 agccctaaaa gcaattaagg cggttaatgt taatccaact gatggtgaga cggccattaa
    17041 cgaaatgaaa agcttagggg ttcaaatcgt ttgaaagtaa aaattagaag gggacttttt
    17101 taagccccct tcattcattt cattagttct tttttaacga gtaacagtta acttacgggt
    17161 tgcaaccgta ccattatcgc ctttaatttt gataagatac acacccgtag aaagattcga
    17221 tacatctatt ttcccttctg ttgtttctgt tcccgaggtc cataccaatg ctccagtaat
    17281 gttgtaaaca tcaattatac cttttgtaaa cccggcaatg ttaacaaaaa ccgatgaatt
    17341 ggttgggttt ggataaacag taagtttatt atcactattg ttagtttcaa cccctgaggg
    17401 ccaggtaacg tttaagactt tctctttaac atatacatga atatcgttac ctttttgaac
    17461 ggttaaggtt atcgttttat ctccgtaagt ggtgtattta acggtatgtg gacccttacc
    17521 tgtccttgta gcaggttcag cgccctcgcc aaagttccac tcccacgatt ctggcgagcc
    17581 ataggtcata tcggtaaata caaccgtact gtcgttgcga taagcagtag ttttatttac
    17641 ggtgaaatta gctgaaagta ccgatgcccc tttcttccac attccaccat agttagcact
    17701 tgagttaaaa cttccagccc atactgtgcc atttggcgag gcacccatat ttgtaaactg
    17761 gaaattcttg taaaagggtg cataatcggt aaacgtatta ccgccatcaa cactatagga
    17821 tacgcccatt ttattggcag agtagttgga accagtcgat attaaagtat cagtacctgg
    17881 tatccagcat aaatcagcag tgtatacact tccgcttggg gtgaattcag tccaagtagt
    17941 tccaccgtca gtggttctat aatacttatc aatgttattt tcccatccat aaataacacc
    18001 ttcattgcta ttcttgaatg caatttcaaa ggcagttgaa gttaatgggg tttggtatac
    18061 ctgccagctt gcgcctttat ttgttgactt atatattcta cctttatttg tggcaaacca
    18121 aacattatcg ccaataacat catagtagcc aactacgccg tattcaccat tgaggggtgc
    18181 tggaatgttg gcttgggata ctcttgtcca gtttgttcca ccattggttg tggtgtatat
    18241 ttcaaagtat cctccgttag ggtcgcccat acagaagccg ttatcagcat cccagaaatg
    18301 aacaacattg gggaagccgt ttggcttagt gaaagttgcg gaagtttgct gtgtccaggt
    18361 ttttccacca tcggtggttt tggctatcat tccaccccca ttcgtcccat catataaggg
    18421 cacccaagcg gttttgtcat caattgcgca tatcatactc gctgcaaacc ctgcagagtt
    18481 tgcagggtta attgaacctg ctttccacgt agctccacca tcagtggtca tggtatactc
    18541 cttaacctta gcattaccac ccgaaccgtc gaaagccact gcccatgcaa ccctattact
    18601 tactgccgat atgtactgaa ttccgcgcga tgaggttcca aaaccgcttg cttgccgtac
    18661 ccaaaggtgt ttaaattctg attggtttgg ttggatgccg ataatcatat cgttactttc
    18721 gctaaaatcg taactagttc cgctgggtat catagctgtt gcagcatagt atccatttgc
    18781 atatccaccc catccccagt taatgtgtag ctcgttattg tcgttataac catcgcaaac
    18841 ccatgcgtga ccgccactag ctgaactgct acccgaatag taaattggcc tgccagcatc
    18901 aatttcggtt ttcaccatat ttatccattc ggtttggtta tcagggttaa aggtgtaaat
    18961 ttgaatagtg gttgggtcgt agttaaagta gtttgtaagc gcgtacaaaa catctctgct
    19021 aaaggcgcct gaaccatcgg tcgcatagtt catatttact gaaactccgg catggtagca
//
LOCUS       DAOSAL010000051        18570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_2084_length_18570_cov_53.367378, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000051 DAOSAL010000000
VERSION     DAOSAL010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 18570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18570
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2084_length_18570_cov_53.367378"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..492
                     /locus_tag="PK049_07900"
     CDS             103..492
                     /locus_tag="PK049_07900"
                     /inference="COORDINATES: protein motif:HMM:NF012527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61520.1"
                     /translation="MRKVAGTLKLDQAQYRELEAFAKFGADLDAATLAVLDKGAKNVE
                     ILKQGQYQPLPVELQVAIIFCGTKGLLKNIPVAKVRDFEKDFLERCQLLLKAELEQIA
                     KGEFNDQIADMLSNLAEEVAKNYTEFK"
     gene            502..1380
                     /gene="atpG"
                     /locus_tag="PK049_07905"
     CDS             502..1380
                     /gene="atpG"
                     /locus_tag="PK049_07905"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HOK61521.1"
                     /translation="MANLKEIRTRIASVASTKKITSAMKMVSAAKFKKAQINVLRFRP
                     FNDQVNNILQSSIEFIGSLSGDYVNERQQVKNIVLIVITSNNSMCGAFNSNIIKYSIE
                     QYKSISARHPSARVTFYTLGKKGHEALSRRGYNVIELNHKMLDKPNIENTKDIFNTLV
                     NEFKHGSADKIFLIYNMFKSAASQQQVAEQLLPINILRTAKKTNKIKPIIEPNPQVLV
                     ERILPYFLLNKIHSAILESVAAEHGARMTAMHQATENAVALHNQLLLEYNKARQAAIT
                     KEILEIVSGANALSSK"
     gene            complement(1423..3153)
                     /locus_tag="PK049_07910"
     CDS             complement(1423..3153)
                     /locus_tag="PK049_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005863486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HOK61522.1"
                     /translation="MNYGLLDLLNLIGSLGLFLYGMKMMSESLQKVAGDKMRAILSSM
                     TSNRFMGILTGFLVTAVIQSSSATTVMVVSFVNAGLLSLVQSIGVIMGANIGTTITAW
                     LISLLGFKFSIILIAVPLIGVGFPLLFSRKQNLKFWGEFLIGFSLLFIGLEFLKNSVP
                     RVDQNPEMFAFLSNYTDMGFLSILLFLGIGILLTIIIQSSSATMALTLVMCNQGWISF
                     EIAAAMVLGENIGTTITANIAAIIGNVSAKRAARAHFFFNVIGVIWMLLIFYPFTRTI
                     ANVLVDITGKSPYTDTVEIPFALSIFHSSFNLINTFLLVWFTPLLVKLVTWLTPSKKE
                     EDEEFRLVHINAGLMTTAELSLVQAHKEVITFAKRTHRMFGFVRQLFNETNDKEFDNI
                     YQRIEKYEGISDRVEVEIATYLNKVSTYELSDESSRKLQALFRIISEIESVADSNFSL
                     ARTLKRKREQKVWFNQEMRDNLNKMFDMLDEAYNIMKENLEISQRSVSINLGPVYEVE
                     SKINEFRIFLKEDHIRSIEANKYKYQAGVIYSDLFNEAEKMGDYIINVSEAIAEIEKP
                     VKHKSYPIGK"
     gene            3330..5681
                     /locus_tag="PK049_07915"
     CDS             3330..5681
                     /locus_tag="PK049_07915"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOK61523.1"
                     /translation="MFHKLARFILKRRFILVSILLVFTFFMGWKATKVRLSYKPIPLV
                     PKTDSIYIQNERLAKLFGKGENIMVIGVTDSAFFSSDRVLLWENLSNKIKQVDGVENV
                     FSVIDIITLEKDTITRKFVAKKLLDNLNTISPDSLSNQVKNLPFYKGLLYNAETNTYL
                     MLITLNREKMTRVDRIKIINQVKEASQEYEARTGNTLHYSGLPYIRAVVAEMIKYEMF
                     LFVGLAALVTAIILLVLFRSFRVLFISLVVVGTAVIWSLGSLTLFGYDLTLLTAVVPP
                     LLIVIGVPNCIYLINKYHHEIAHHRNKIKALYRVIYRIGSATFLTNLTTALGFVTFVF
                     TKTQILQEFGLIAFLNVLGIFLISLIVIPFFFSISPTPKARHLSHLNRNTFKQAVSRI
                     VLTTYYKRKWVFAITVFALVIGIFGASLLKANGYMVDDVPQNHPVQRDLKFFEKNFTG
                     IMPLEIVYNTGKPNGYQNYTVYQKVERLQEKLSSYPELSKPFSIAEIAKFARQAYYNG
                     NESQYRLPGPFERAFIMSYVPRSIGVSDVYARMTDSTGQYVRIMYNVADIGSEKMSIL
                     FDSINSDIRQVFDDEHTNVHIAGASLITTYGIKYLVHSLSSSLIFAIILIALAIAWLF
                     RKFRMVLFAIGINLIPLILTAATMGFLGINLKPSTVIVFSIALGIAVDNSIHFLTKYR
                     QELYLTKFNIKESIFYAIRETGISMIYTSIVLLFGFSSFIASRFGGTKALGLLVAITL
                     LYAVITNLFVLPSVLRQFDKPKPLKPEEEELVMEMSSGFDDEE"
     gene            5694..6668
                     /locus_tag="PK049_07920"
     CDS             5694..6668
                     /locus_tag="PK049_07920"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOK61524.1"
                     /translation="MYTLESINQKVDSAIKQLDLTGEPRNLYEPIAYIMSMGGKRIRP
                     ALVLAACNLYSDDIDQALPAALAVELFHNFTLVHDDIMDNADMRRGLVTIHKKWNPNI
                     AILSGDAMTVLAYKLLVQVDKSILPEVINIFNSFAMGICEGQQMDMDFESDTMVSRDE
                     YLKMIELKTSILLKGALQIGAVIGGAGRNDVELIGEFGRSMGLAFQLQDDLLDCYGDS
                     STFGKRIGGDIVASKKTMLTIITAEKLKGTQRSDFIELYNSKSLTDQLKIGRVMDYYS
                     KAEVQHEMVSIIDTYFDNATQAINKLNVSESRKDVLNQILTRLIGRKN"
     gene            6749..7588
                     /locus_tag="PK049_07925"
     CDS             6749..7588
                     /locus_tag="PK049_07925"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOK61525.1"
                     /translation="MAENINKYLIPWDFTPVAENALKHAIKLMKLAKEPIEINLVHVI
                     QTGGLFSKSKFSEDDASQKLKDDQQRIVNEYGVEVKTHLLEGNIFDTISEFASEINAE
                     MVIMGTHGIKGVQKLTGSWALKVIAGSEVPFLVVQDEPDMAKGFSHIVMPVEFRDEDK
                     EKIQWGIRFAKLFGSKIHVIVPNAFDSGVQKKINNNLSFAKMHIESNGLDWEIHNAAK
                     GKSFQEEIVNLATDIEAELIIVMTTPNIDLTDFVFGAQEQYVIANKAKIPVLCINPGT
                     LGV"
     gene            complement(7563..8603)
                     /gene="mnmH"
                     /locus_tag="PK049_07930"
     CDS             complement(7563..8603)
                     /gene="mnmH"
                     /locus_tag="PK049_07930"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HOK61526.1"
                     /translation="MASTISIDKFIELSDIYPVVDVRSPSEYARGHIPGAFNIPLFSD
                     EERAAVGTKYVREGRQQAVRLGLELVAPKLNWFVDSLSSMVNQKNILVYCWRGGMRSA
                     SMSWLFGLAGFKPFTLEGGYKSFRRYAQAAFDKKYKFIVLGGMTGCGKTELLKKLKEN
                     GEQVVDLEGLANHKGSAFGWINQPQQPTTEHFENLLFNELRKLNPALPIWIEDESKSI
                     GSIFIPLSIYNQIISSPTIIVETDLKSRIDRLISDYTACDKQDLINSVVRIQKRLGLE
                     KAKRCIEYIESGNLREAIAITLEYYDKTYSYGIEQKKIPPKFLTFDGTSNDMIVKLTS
                     VGKSITHPAFPD"
     gene            complement(8606..9373)
                     /locus_tag="PK049_07935"
     CDS             complement(8606..9373)
                     /locus_tag="PK049_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016110865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HOK61527.1"
                     /translation="MKSHEIDYRIIGESIQLVEIELDPHETVIAEAGAMVYMESGIDF
                     ETKLGDGSNPSSGFLGKLLSAGARAITGESIFMTHFTNHGIGKRKVAFSAPYPGTVVP
                     IDLSKVGGNLIVQKDGFLCAALGTSISLHFNRKLGAGLVGGEGFILQRLSGDGLAFVH
                     AGGTVIEKQLNNETLRIDTGCIVAFEPTIDFDVQTSGNLKSMLFGGEGLFLATLSGSG
                     KVWLQSMPIKKLVQALAPYGRNRGKENSSILGNLLED"
     gene            complement(9417..10352)
                     /gene="rsgA"
                     /locus_tag="PK049_07940"
     CDS             complement(9417..10352)
                     /gene="rsgA"
                     /locus_tag="PK049_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005633200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOK61528.1"
                     /translation="MEKGLVLKSTGSHYRVRLDDGRVVECVPRGKLRLNEIKTTNPIT
                     VGDWVSVELNPNGVGLITAIQERKNYIIRKATNLSREAHIIAANIDQALLVVTITQPE
                     TQLAFIDRYLVTAEAYRIPTVIVFNKIDLVDSAIQPVLDSYISIYEPIGYKCLKVSAK
                     TGQNLDLLKQILQGRVSLLSGNSGVGKSTLINLIEPGLNLKTAEISNAHLKGRHTTTF
                     SEIFELSFGGFIIDTPGIKSFGLVDIEKDELYHFFPEIFKLSDKCKYYNCTHIHEPGC
                     AVIEAVDKSEIAPSRYLSYLSIYDDENEKYRPKLK"
     gene            complement(10363..11274)
                     /locus_tag="PK049_07945"
     CDS             complement(10363..11274)
                     /locus_tag="PK049_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOK61529.1"
                     /translation="MTYVTKEKLFSREWFKAYALILVGSFILAAGFVLFIDPHRIAPG
                     GVYGIGIIVHYLTKGMFSWAPDGFPIGTVGLLLNIPLTIIGIRVLGPMFGIKTVVGFV
                     LSSVFIDTLHLWLGYDPLVDDMLLSSIFGGVLIGFGLGLIFKSKATSGGSDIIAMIIA
                     KYTRLPLGQLMIYVDSTIVLLALVAFQDWKIPLYSWVVIYVTGKVIDMTIQGVSYDKA
                     LFIITDRHEEVKNKIIHDIERGGTIINVQGMYTGHDKKMIFTNVSRREVHMLKDYIQQ
                     IDPNAFITVIDANEILGNGFKPLNESM"
     gene            complement(11288..12364)
                     /locus_tag="PK049_07950"
     CDS             complement(11288..12364)
                     /locus_tag="PK049_07950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016274675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOK61530.1"
                     /translation="MEQNSKKVNGYPKKLLVVRLSAIGDVAMTVPAIWSLVKNHPDVQ
                     VTFVSQGFARDIVNQIPGVLFVEANTKGRHKGLLGIWRLFTDLRKIDRYDALADLHNV
                     IRSQILRNLFLLSGLKKISVINKGRSEKRKLVRLKNKRLAPLKTTPERYMDALLRLGY
                     TINDEFDYLFREARLPENITIHLGYKDKRWIGIAPFAKHKGKIYPLDKMERVVEILSA
                     SKTNKIILIGGGGKEQEILEGWEKKYPNTISIARKYSILNELKIISNLDVMVCMDSAN
                     MHFASLVNTPVVSIWGATHPYAGFYGWKQNPTSAVQVDLYCRPCSIYGNKPCFRKDYA
                     CLNLIEPEVVVSKINTVIDRFNRI"
     gene            complement(12339..12956)
                     /locus_tag="PK049_07955"
     CDS             complement(12339..12956)
                     /locus_tag="PK049_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007756356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4254 domain-containing protein"
                     /protein_id="HOK61531.1"
                     /translation="MSFSQLCNKIFWDCTLHYHTMDNVDAQPNIPYQPGTIEFSLFTK
                     NWIDAVQWHLEDIIRDPKIDPVEALKIKRRIDKSNQDRTDLVEMIDSYFLEKYKDIKP
                     QSNATINTESPAWAIDRLSILALKIYHMEQEVKRTDVSKEHIDSCQKKLNTLLEQRVD
                     LSTAIDQLLDDIEHGRKYMKVYKQMKMYNDPSLNPVLYGAKQQKG"
     gene            complement(13001..14251)
                     /locus_tag="PK049_07960"
     CDS             complement(13001..14251)
                     /locus_tag="PK049_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HOK61532.1"
                     /translation="MLKREDIEVMAPAGNFESLMAAIQGGANSVYFGVGNLNMRAKSS
                     INFTVDDLPEVVRICKKNKVKTYLTVNTVIYDSDIPFMHQIIDTAAEAGVSALIISDQ
                     AAMLYARKRGMEVHISTQLNISNTDALKFYSQWADVVVLARELNLEQVKTIYQNIHSQ
                     NITGPNGNPIKIELFAHGALCMAISGKCYLSLHEANSSANRGACLQICRRSYTVTDNE
                     TGAQLEIDNEYIMSPKDLCTINFLDKILDAGVRVLKIEGRARGPEYVKTVTQCYNEAV
                     TSVCNGTYTPEKVENWMQRLSTVFNRGFWDGYYLGRRLGEWSKAYGNIATERKQYVGK
                     ITNYFSRLGVAEITVEASELYLNDKILIIGPTTGVIEYEVKELQVDEKPAEFSPKGVK
                     CSIPVPDKVRRADKLYRIVKMNSD"
     gene            complement(14348..15220)
                     /locus_tag="PK049_07965"
     CDS             complement(14348..15220)
                     /locus_tag="PK049_07965"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOK61533.1"
                     /translation="MKRFIFLVALLNVLIISTNAQNKTKSQLEADFEKFKKEREQDFQ
                     DFKQKREAELKKMEQEYLDYYNEMTGLKKHYVEKKEPEKAQTVQDIIDYENTIAKTLG
                     YKIPERVKEKVEANTKSEVSKKEEAKTEAENVNVEDKKPEVSSSTFKPEAGTRDGIPV
                     LTPLPKRLAKITSPFGVRIHPTLGRPIKHNGVDFGSGRGAEVYAASSGKVVLAQYNST
                     YGNYIIIEHNDGTSSVYAHLDKIKVSKGSPIRKGDVIGYTGSTGRSTGPHLHYEVRIK
                     GIPVDPKGYLVETK"
     gene            15394..16302
                     /locus_tag="PK049_07970"
     CDS             15394..16302
                     /locus_tag="PK049_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005792036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOK61534.1"
                     /translation="MKPTLVILAAGMGSRYGGLKQVDALGPNGETIIDYSVYDAIRAG
                     FGKVVFVIRKSIEKDFLEVFGNRFDSDVRVEIAFQELDMLPQGFKVPDGREKPWGTAH
                     AVWVTRHLINEPFAVINADDFYGFDTFRVLAQELSKPNLEKGSYFMVGYKLGNTLSEQ
                     GAVSRGVCSTDKNGYLLDVVERTHIERVEGEVKYKDENGNFVKVDEGVPVSMNFWGFT
                     TDFFGYTEKLMTDFFSNQIGNVKAEFYIPTVVNRAIKEGIARCKVLETKAEWFGVTYP
                     GDRPMVVNRLKELTEKGVYPSPLWSR"
     gene            16402..18000
                     /locus_tag="PK049_07975"
     CDS             16402..18000
                     /locus_tag="PK049_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005940763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/sugar symporter"
                     /protein_id="HOK61535.1"
                     /translation="MSFATVDYIVFIAYALLIVSLGLWVSFRKRDSETNSGDYFLAGR
                     ALPWWAIGASLIASNISAEQFIGMSGSGYAIGLGIASYEWMAALTLILVAVFFLPIFL
                     EKRIFTMPQFLEVRYDKRVKTVMAGFWLLVFVFVNLTSIIYLGALAMQRIMGINMTYA
                     IIGLAFFAALYSIYGGLKAVAWTDVVQVVFLVGGGLVTTYFALDYLSDGHGFIAGFKA
                     LLQKAPEKFDMILEKGKLMIPDGKGGQKDAYIDLPGISVLIGGMWVANLYYWGCNQYI
                     IQRALAAKSIKEAQSGLVFAAFLKVLLPLIVVIPGIVAFALHADIAKSDEAYPWLLHN
                     FVPTGVKGLAFAALVAAIVSSLASMMNSVSTIFTMDFYKEYINKDASEHKLVTVGRIA
                     AFVALLIAVFIAPMLSNLDQAFQYIQEFTGFVSPGALAIFLAGFFYKKATANGALGAA
                     VGTFVFSLLFKLLLPNLPFMDRMGIVFLICLAIIWIFAMVEGNKQHVKAITISKELFS
                     TGKGFKIGSAIIIALLIILYAIWW"
     gene            complement(18073..>18570)
                     /locus_tag="PK049_07980"
     CDS             complement(18073..>18570)
                     /locus_tag="PK049_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005676883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOK61536.1"
                     /translation="ELDVVISVGSFLEGDYFTVFSELQQIKEVAENAKVKVILETGAL
                     PSLEDIKLASFLAMEAGSDFIKTSTGKLEPAATPEAVYVMCLAIKEFHEKTGRKVGMK
                     PAGGVSNSYDAALFYTIVEQVLGQEWLYPGLFRFGTSRLADVLLSDILGKDVKYFSTS
                     SSAKY"
BASE COUNT         5425 a         3710 c         3787 g         5648 t
ORIGIN      
        1 caaatattcc ttgaatcgaa cctgttcaac aacggtatta ggcctgccat caatgtaggc
       61 atctcggttt cgcgagttgg tggaaaggca cagataaaat cgatgcgcaa ggttgccggt
      121 actcttaaat tggatcaggc tcagtatcgc gagcttgaag cttttgccaa gtttggcgcc
      181 gatttagatg ctgctacgct tgccgttttg gacaaggggg ccaaaaacgt tgagatacta
      241 aaacaggggc aataccaacc tttgccggtt gaactgcaag ttgcaatcat tttctgtgga
      301 acaaagggtc tgcttaaaaa tatccccgtt gcaaaagttc gtgattttga gaaggatttc
      361 ctggagcgtt gccagctttt actaaaagcg gagttggagc aaatagcaaa gggcgaattt
      421 aatgaccaaa ttgccgatat gctgtcaaac ctagccgaag aggttgccaa aaattacacg
      481 gaatttaaat aaatttctgg gatggccaat ttgaaagaaa tacgaaccag aatagcatcg
      541 gtagcttcca caaagaagat taccagtgca atgaaaatgg tttcggcagc caaattcaaa
      601 aaggcgcaaa ttaatgtgtt acgttttaga ccttttaacg accaagtaaa caatatatta
      661 caatcatcaa ttgaatttat tggttcatta agtggcgatt atgtaaacga aaggcaacaa
      721 gtaaagaata tcgttttgat tgttatcacc tccaacaata gcatgtgtgg agcattcaat
      781 tcaaatatca tcaaatactc aattgagcaa tataagtcaa tatcggctag acacccaagt
      841 gccagggtaa cattctatac tttaggtaaa aagggacacg aagcactaag ccgaaggggt
      901 tataatgtaa ttgagctaaa ccataaaatg cttgataaac ctaatattga aaacacaaaa
      961 gatattttta ataccctggt taacgagttt aagcatggtt cagccgataa aatctttcta
     1021 atttacaata tgtttaaaag tgctgcatcg cagcaacaag tagcagagca gttacttcca
     1081 ataaatattt taaggacagc taaaaagaca aataaaatta aaccaataat tgaacctaac
     1141 ccacaagttc tggttgagcg tattttgcct tactttcttt tgaacaagat ccattcggca
     1201 attcttgaaa gcgttgcagc cgaacacggt gctcgtatga cagccatgca tcaggccaca
     1261 gaaaatgctg ttgcattaca caatcagtta ctccttgaat acaataaagc acgccaggct
     1321 gcaattacta aggagattct tgaaattgta tcgggtgcaa acgcattgag cagtaagtaa
     1381 aaaagcgggc tacatcccgc ttttttatat cagctaatta aactatttgc cgatagggta
     1441 ggatttatgc tttacaggtt tttcaatttc agcaattgcc tcagaaacat ttatgatgta
     1501 atcgcccatt ttttcggctt cgttaaataa atcgctataa ataacaccgg cctgatactt
     1561 gtacttattg gcctcaatgc ttctgatatg atcctcttta agaaagatac ggaactcgtt
     1621 gatcttgctt tcaacctcgt aaactgggcc taagttaatg gaaacgcttc gctggctaat
     1681 ttcaagattc tccttcatga tgttatatgc ctcatcgagc atatcaaaca ttttgtttag
     1741 gttatcgcgc atttcctggt taaaccaaac cttttgttca cgcttgcgtt ttagggttcg
     1801 ggctaaagaa aagttgctat cggcaaccga ttcaatttcg ctaattattc taaataaagc
     1861 ctggagttta cgtgaacttt catcgctaag ctcgtaggtt gaaaccttat taaggtaagt
     1921 ggcaatttct acttcaaccc tatcgcttat tccttcgtat ttttcgattc gctgatagat
     1981 attgtcgaac tccttgtcgt tggtttcatt aaaaagttga cgaacaaaac caaacattcg
     2041 gtgagtgcgc ttggcaaaag taataacctc tttatgcgct tgcaccaatg agagctcggc
     2101 tgtagtcatt aacccggcat tgatgtgaac caacctaaac tcttcgtcct cttccttttt
     2161 cgaaggggtt agccatgtta ccagtttaac gagcaaaggt gtaaaccaaa cgagtaagaa
     2221 ggtgtttatc agattaaacg atgagtggaa aatgctaaga gcaaagggta tctcaactgt
     2281 atcggtataa ggcgatttac ctgtaatatc aactaaaaca ttagcaatgg ttcgagtaaa
     2341 tgggtagaaa attaacagca tccaaattac accaattaca ttaaaaaaga agtgagccct
     2401 tgctgcgcgt ttggctgaaa cgttaccgat aattgcggca atatttgctg tgatagttgt
     2461 tccaatattc tcaccaagca ccatggctgc tgcaatttca aatgaaatcc acccctggtt
     2521 gcacataact agcgttaacg ccatggttgc gctcgacgat tggattatta tggttaaaag
     2581 tataccaata cccagaaaga gtagtatcga caaaaagccc atatcggtgt aattggaaag
     2641 gaatgcaaac atctcagggt tttggtctac tcgaggaacc gaatttttaa gaaattccaa
     2701 accaataaaa agcaacgaga agccaataag aaactctccc caaaacttga ggttttgctt
     2761 acgggaaaaa aggagaggga aacccacccc aattaaaggt actgctataa gaataatgct
     2821 aaacttgaaa cccaaaagcg atattagcca tgccgtgatg gttgtaccaa tgtttgcacc
     2881 cattataacg ccaatagact gaaccaacga tagcaaaccg gcgttaacaa agctaacaac
     2941 cattacagtt gtagctgatg acgactgaat tactgcagtt acaagaaagc ctgttaggat
     3001 tcccataaac ctgttggatg tcatggaact caagatggca cgcatcttat cgcctgctac
     3061 cttttggagc gattcgctca tcattttcat tccgtaaagg aatagtccca gcgaaccaat
     3121 taagttcaaa agatcaagaa gtccgtaatt cattggtata ttttggttta aataactaga
     3181 aataattaaa aatcgcaacc aattttttgc aaaggtaact tttaccagta taaatcaaaa
     3241 atcaatattt tatgtattga ttagctatag ttgcaacaac atatccatat tatttctaat
     3301 attgctgatt gaatcctaat agtaaagcaa tgtttcataa gcttgcccga tttattctta
     3361 agagacggtt tatccttgta tcaattttac tggtatttac ttttttcatg ggatggaaag
     3421 caaccaaggt aaggctatcc tataaaccca tcccccttgt tccaaaaacc gatagcatat
     3481 atatccaaaa tgagaggctg gcaaaacttt ttgggaaggg tgagaatata atggttattg
     3541 gggtaacgga ttcagccttt tttagtagcg atagagtttt gctttgggag aatttgtcca
     3601 ataaaatcaa gcaagtagat ggtgtagaaa acgttttctc tgttatcgat attataacgc
     3661 tggaaaaaga tactattacc aggaagttcg tagcaaaaaa gttgctggat aaccttaata
     3721 cgattagtcc ggattccctt agtaatcagg ttaaaaactt accattctat aaggggctgc
     3781 tttataatgc tgaaaccaac acctacctaa tgcttataac cctgaacaga gaaaagatga
     3841 ccagggtaga ccgcattaaa atcataaacc aggtaaagga agcatcacaa gaatacgaag
     3901 ctagaaccgg aaacacactc cactactctg gcttacctta cataagagca gtggtggccg
     3961 aaatgattaa atacgaaatg ttcctatttg ttggccttgc tgcactggtt actgcaatta
     4021 ttcttttagt gcttttccgc tcattccgcg tgctatttat ttccttggtg gtagtgggta
     4081 ctgccgtaat atggtcgtta ggttcactta cacttttcgg atacgattta acgttgctaa
     4141 cggcagttgt gccccctttg ttaatagtga taggggttcc caattgtatc tacctaatta
     4201 ataagtacca tcatgaaata gcccatcacc gtaataaaat caaagccctt taccgcgtta
     4261 tatatcgaat tggcagtgca acgtttttaa ccaatttaac taccgcatta ggttttgtga
     4321 catttgtttt cactaaaaca caaattttac aggagtttgg tttgattgcc tttttaaacg
     4381 tcctaggtat ttttcttatt tcattaattg ttataccttt cttttttagc ataagcccta
     4441 cacccaaagc tcgacactta agtcatctta atcgaaatac ttttaaacag gcggtttcac
     4501 gtatagtatt aaccacctat tacaagcgca aatgggtttt tgccattacg gtttttgctt
     4561 tggttattgg aatatttggt gcttccctct tgaaggcaaa cggttacatg gttgatgatg
     4621 tcccccaaaa tcacccagta cagcgcgact tgaaattttt tgaaaaaaat tttacgggga
     4681 ttatgcccct tgaaatagta tataacactg gaaaacctaa tggttaccaa aactataccg
     4741 tttaccaaaa agtagaaaga ttacaggaaa aacttagctc ttatccagag ctttcaaaac
     4801 ctttttctat agccgagata gcaaaatttg cccgacaagc ctactataac ggcaacgaga
     4861 gccaataccg gctacccgga ccctttgaac gagcttttat aatgtcgtat gttcctcgaa
     4921 gcatcggtgt tagtgatgtt tatgcccgaa tgacagactc aaccggccag tatgtaagga
     4981 taatgtacaa tgtggccgat attggttctg aaaaaatgag cattttgttt gacagcataa
     5041 acagtgatat taggcaggtt tttgatgatg agcatacaaa tgttcatatt gcaggggcaa
     5101 gcctaattac tacctatgga attaagtatt tagtccatag tttatcatcg agcctaattt
     5161 ttgcaataat acttattgcc cttgccatag cttggctatt ccgtaagttt agaatggtac
     5221 tttttgccat tggaataaat cttattccgc ttatactaac tgcagccact atgggttttc
     5281 tgggtatcaa cctaaaacca agtaccgtta ttgtttttag tattgcttta ggtattgctg
     5341 ttgacaactc aatacacttt cttaccaagt atcgccagga attgtattta accaagttca
     5401 atataaaaga gtcaatattt tatgcaattc gtgaaacagg cattagcatg atttatacat
     5461 caatagtatt acttttcgga tttagttcat ttattgcttc acggtttggt ggtacaaaag
     5521 ctcttgggct tttagttgcc ataaccttgc tatatgcagt tattacaaac ctatttgtac
     5581 ttccttcggt tttacgtcaa tttgataaac ccaagccatt aaaacctgag gaggaagaac
     5641 ttgtaatgga aatgagtagc ggtttcgacg acgaagagta aaaatcaacc agaatgtata
     5701 cacttgaatc aataaaccag aaagttgact cagcaattaa gcagcttgat ttaacaggtg
     5761 aaccaagaaa cctttacgag ccaatcgcat acataatgag tatgggtggt aaacgaattc
     5821 gcccagccct tgtacttgca gcgtgtaatc tttactctga tgatattgac caagcattac
     5881 ctgcagctct cgccgttgag cttttccata attttacctt ggtccacgac gatattatgg
     5941 ataatgctga tatgcgtaga gggttggtaa ctattcataa gaagtggaac cccaatattg
     6001 caattctttc aggtgacgcc atgacagtgc tagcctacaa gttgctcgtt caggttgata
     6061 agtcgatttt gcccgaggtg attaacattt ttaactcttt tgccatgggt atatgcgagg
     6121 gccaacaaat ggatatggat tttgagtcag acaccatggt ttctcgcgat gagtacctta
     6181 aaatgataga gctaaaaaca tcaatattac ttaaaggggc tctgcagatt ggtgcagtaa
     6241 ttggcggcgc aggcaggaat gatgtagagc ttattggtga gtttggcagg tcgatggggt
     6301 tggcttttca gcttcaggac gatctgcttg actgttatgg cgattcaagt acttttggta
     6361 aacgcattgg tggcgacatt gttgcatcaa aaaaaaccat gttaaccatt attactgcgg
     6421 aaaagctaaa gggaacacaa agaagcgatt tcattgaact atacaacagt aaatcattga
     6481 ctgatcagct aaagattggc agggtaatgg attattacag taaagcagaa gttcagcatg
     6541 aaatggtaag cattatcgat acatactttg acaatgccac gcaggccata aataagctta
     6601 atgtaagcga atccagaaaa gatgtactta atcaaatatt aacaaggtta attggccgaa
     6661 aaaattgaat attggtttaa aggattgatt ttaaattaaa attttgtaaa tttaactacc
     6721 caatttttag tactaaaatg taaaacctat ggcagaaaat attaacaaat atctaattcc
     6781 ttgggatttt acccctgttg cagaaaatgc attaaagcac gcaataaaac tgatgaagct
     6841 tgctaaggaa cccattgaaa tcaaccttgt ccatgttatt caaaccggcg ggttattcag
     6901 taaaagtaag tttagcgagg atgatgcaag ccaaaagctg aaggacgatc agcaaagaat
     6961 tgtaaatgag tatggtgttg aggttaaaac tcacctttta gagggcaata tttttgatac
     7021 cataagcgag tttgccagtg aaataaatgc cgaaatggta attatgggta ctcatggaat
     7081 taaaggagta caaaagctca ctggaagttg ggcgctaaag gttatagcag gctccgaggt
     7141 gcctttcttg gttgttcagg atgagcccga tatggcaaag ggattttcac atattgttat
     7201 gcccgttgag ttcagggatg aggataaaga gaaaattcaa tggggaattc gttttgctaa
     7261 gctgtttggt tccaagatcc atgtgattgt tccaaatgct ttcgattcag gcgttcagaa
     7321 aaaaattaac aataacctat cttttgccaa aatgcatata gaatccaatg gtctggactg
     7381 ggaaattcat aatgccgcaa agggtaaaag ttttcaggag gaaattgtta acctggcaac
     7441 cgatattgag gccgaactga taattgtgat gacaacccct aatatcgacc taaccgattt
     7501 tgtttttgga gcccaggagc aatacgtaat tgccaataaa gccaaaatcc cagtactttg
     7561 cattaatccg ggaacgctgg gtgtgtaatg cttttaccaa ccgacgtcag cttaacaatc
     7621 atatcgttgc ttgtaccatc aaaggttaaa aactttggtg gtattttctt ttgctcaatt
     7681 ccgtaactat aggtcttatc gtaatattcc agcgtaatgg caatagcttc gcgcagattt
     7741 ccagactcga tatactcaat acaacgtttt gccttttcta accccagtcg cttttgtatt
     7801 cgcacaaccg agttaattaa gtcctgttta tcgcatgcag tgtagtcgct aatcagcctg
     7861 tcgattctac tttttaggtc agtctcaaca ataatggttg ggctactaat aatttggtta
     7921 taaatagata ggggtataaa aattgaacca atacttttgc tctcatcctc aatccagata
     7981 ggtagtgcgg ggtttaactt tcgtagttca ttaaacagta gattttcaaa gtgctcagtg
     8041 gtgggttgct gcggctggtt tatccagccg aaggcagaac ccttgtggtt ggcaagccct
     8101 tccagatcaa caacctgctc accattttcc ttgagttttt taagaagttc ggttttaccg
     8161 cagccagtca tgccgccaag aacaataaat ttatatttct tgtcgaaagc agcctgtgcg
     8221 tatcgcctga atgacttata accaccttcc aacgtaaagg gcttaaaacc agctaagcca
     8281 aaaagccagc tcatgctggc acttcgcatt ccaccacgcc agcagtaaac cagaatgttt
     8341 ttttggttaa ccattgaact taatgaatca acaaaccagt ttagcttagg agcaaccagt
     8401 tcaagtccca gtctaacggc ttgttgtcgt ccttccctta catatttagt tcctacagca
     8461 gcgcgttcct cgtcggaaaa aaggggaata ttaaatgcgc ccggaatatg cccacgagca
     8521 tattccgaag gtgagcgaac atcaacaaca gggtaaatat ctgatagttc aataaattta
     8581 tcgatactaa tggttgaagc catttttaat cttctaaaag attgccaagt attgaactat
     8641 tttcttttcc tctatttctg ccgtaaggag caagagcctg aaccagtttc ttgataggca
     8701 tggattgaag ccaaacctta ccggaaccgg aaagggtggc aaggaataaa ccttcgccgc
     8761 caaagagcat gctttttaga tttccgcttg tttgaacatc gaaatcaatt gttggctcaa
     8821 aggcaacaat gcaaccggta tcgattcgta aggtctcatt gttgagctgc ttttcaatca
     8881 cggttccgcc ggcatgcaca aaggctaaac catcgccgct gaggcgctga aggataaagc
     8941 cctcaccacc aaccaagccc gctcccagtt ttcggttgaa atggagcgaa atacttgtac
     9001 caagagccgc acaaaggaac ccgtcctttt ggactataag attacccccc acctttgaca
     9061 aatcgattgg cacaacagtg ccggggtagg gtgcggaaaa agcaactttc cttttgccaa
     9121 taccatggtt agtgaagtga gtcataaaaa tcgattcacc ggttatagca cgtgctccag
     9181 ccgaaagcag tttccctaaa aaccctgaac tgggatttga gccgtcgcca agctttgttt
     9241 caaaatcaat tccactttcc atgtaaacca ttgcacctgc ttccgcaatt acagtttcat
     9301 gtgggtcaag ctcaatttca accaattgaa tggattcacc aataattcta tagtcaattt
     9361 cgtgcgattt cataagcaat tggatttaag ttattaataa acatactaat gatctattac
     9421 tttaatttgg gtcgatattt ttcgttttca tcgtcgtata tgctcaagta gctcaggtag
     9481 cgcgatggtg caatttcgct cttgtcaaca gcctcaataa cagcacaacc gggctcatgg
     9541 atatgggtgc aattgtagta cttacatttg tcggatagct taaatatctc agggaagaaa
     9601 tggtatagct catccttttc aatgtcaacc aaaccaaaac tcttaattcc cggtgtatca
     9661 ataataaatc caccaaagct tagctcaaaa atttcgctaa aggttgtagt atgcctaccc
     9721 ttaaggtgag catttgaaat ttcagcggtt ttaagattca aaccgggctc aatgaggtta
     9781 attaacgtag atttacctac ccctgaattt ccggatagca agcttaccct tccttgaagt
     9841 atttgtttaa gtaaatcgag attttgacct gttttggcag aaacttttag gcacttatat
     9901 ccaatgggtt cgtatattga gatgtagcta tcgagcacgg gttgaatggc tgaatcaact
     9961 aagtcgattt tattgaagac aataacagta ggaatgcggt aagcttcagc ggtaaccagg
    10021 taacgatcaa taaatgccag ttgggtttcg ggctgagtaa ttgtaaccac cagtaaagcc
    10081 tgatcgatat tagcagcaat gatgtgagcc tcgcgtgata ggtttgtggc cttacgaata
    10141 atataatttt ttcgctcttg gatggcggtt attaaaccta ccccgttggg gttaagctca
    10201 acgcttaccc agtcgccaac agtaatagga ttggttgttt ttatctcgtt aagcctaagc
    10261 ttaccgcgtg gtacacactc aaccacgcgg ccatcatcca atcgaaccct gtaatggctt
    10321 ccggtactct ttaacaccaa tcccttttcc atgtgtaaaa agctacatac tctcgtttaa
    10381 cggtttaaaa ccatttccaa gaatttcatt ggcatcaatt acagtaataa aagcattcgg
    10441 gtcaatttgc tggatgtaat cctttagcat atgaacctcg cgacgcgata cgtttgtgaa
    10501 aatcattttc ttatcgtgtc cggtgtacat accttgcacg ttaataatgg ttccgccgcg
    10561 ttcaatatcg tgaataatct tgtttttaac ttcttcgtgc ctatcggtaa taatgaaaag
    10621 ggctttatcg tagctaaccc cttgaatggt catatcaatc accttaccgg taacgtaaat
    10681 taccacccag gagtataaag gaattttcca gtcctgaaaa gctaccaagg ctaaaagtac
    10741 aatggttgaa tcaacgtaaa tcatcagctg gcctaggggt agtcgggtat acttagcaat
    10801 tatcattgct atgatatcgg aaccgcccga ggttgcttta gacttgaaga ttaagcccaa
    10861 cccaaagcca atgagtacac cgccaaaaat tgaggaaagc agcatatcgt caacaagggg
    10921 gtcgtatcca agccaaaggt gtaaagtatc gataaaaacc gatgaaagta caaaaccaac
    10981 taccgtttta attccaaaca ttgggcctaa aacccttata ccaataatgg taagtggaat
    11041 atttagcagc aaaccaacag taccaatagg aaaaccatcg ggtgcccaag agaacattcc
    11101 ttttgtgagg taatggacaa taataccaat accatataca ccaccagggg caattcggtg
    11161 tgggtcgatg aatagaacaa aacctgctgc aagaatgaac gatccaacca gtattaaagc
    11221 atatgcttta aaccattccc tgctgaatag cttttctttg gttacgtaag tcataattat
    11281 tttaatttta aattctgttg aaacggtcaa ttaccgtgtt gattttactc accaccacct
    11341 ctggctcaat aaggtttaag caagcgtaat cctttcggaa acagggttta ttcccataaa
    11401 tggagcatgg cctgcagtaa aggtcaacct gaacggcact tgtggggttt tgcttccatc
    11461 cgtaaaagcc tgcataggga tgtgtagcac cccaaatgct tacaacgggg gtgtttacta
    11521 acgatgcaaa atgcatgttt gctgaatcca tgcaaaccat tacgtcgagg ttcgaaatta
    11581 tctttagttc atttagaatg ctgtactttc gggcaatgga aatggtattt ggatattttt
    11641 tctcccagcc ctcaagtatt tcttgctctt ttccacctcc acctattaga atgattttat
    11701 tggtttttga tgcggaaagg atttcaacaa ctcgctccat tttgtcgagc gggtaaattt
    11761 tccccttatg tttggcaaaa ggcgcaatac caatccaccg cttgtccttg tatccaaggt
    11821 ggattgtaat gttttcgggt aatcgagcct cgcggaaaag ataatcaaac tcatcattga
    11881 ttgtgtaccc tagcctaagg agcgcatcca tatagcgttc aggggtagtt ttaagtggag
    11941 cgagccgctt attctttagc cttacaagtt tgcgcttttc gcttcgccct ttatttataa
    12001 cggatatttt ttttagcccc gagagtaaaa ataagtttcg gagaatttgg ctacgaataa
    12061 cattgtgaag gtcggctaaa gcatcgtacc tatctatttt acggagatcg gtgaacaatc
    12121 gccaaattcc caacaacccc ttgtgtcggc ctttggtatt tgcctccaca aataaaactc
    12181 caggaatttg gttaacaata tcgcgggcaa atccttgcga tacaaaggta acctgcacat
    12241 cggggtgatt tttaaccaat gaccaaattg caggtacggt catggccaca tcgccaatgg
    12301 cgctaagcct aaccacaagt agctttttag gatatccgtt aacctttttg ctgttttgct
    12361 ccatatagta caggattaag cgatgggtcg ttgtacattt tcatctgctt gtaaaccttc
    12421 atgtacttgc gcccgtgctc aatatcgtcg agtagctgat cgattgctgt tgataaatcg
    12481 acccgttgct caagcagggt gtttagtttc ttttggcagc tgtcaatatg ctcttttgaa
    12541 acatcggttc gtttaacctc ttgctccatg tggtaaattt ttagggcaag aatggagagg
    12601 cggtcaatgg cccatgccgg actttcagtg tttatagtgg cgttggattg aggtttgata
    12661 tccttatact tttccagaaa gtagctatca atcatttcaa ccaaatcggt tcggtcctga
    12721 ttggatttat cgatacggcg tttaattttt aacgcttcta ccggatcaat tttgggatcg
    12781 cggatgatat cctcgaggtg ccactgaacg gcatcaatcc agttctttgt aaaaagcgag
    12841 aattcaatgg ttcctggctg gtaaggaatg ttaggttggg catcaacatt atccatggtg
    12901 tgatagtgca gggtgcagtc ccaaaaaatc ttgttgcaaa gttggctgaa gctcatatca
    12961 ataggagttt taattaaaca tctaatttac aaatatttta ctaatcggaa ttcattttta
    13021 ctattcggta aagcttatcg gctctgcgca ctttatctgg aaccggaata gaacatttta
    13081 ccccttttgg tgaaaactct gctggttttt catcgacctg aagttccttt acctcatatt
    13141 caataacgcc ggttgttggg ccaataatta gaattttgtc gttgaggtaa agctccgagg
    13201 cctcaacggt tatttcagct acacccagcc tagagaagta gttggttatt tttcctacat
    13261 actgtttgcg ttcagtggca atgttgccat aagccttgct ccactcgcca agcctgcggc
    13321 caagatagta tccatcccag aaaccgcggt taaataccgt agaaaggcgc tgcatccaat
    13381 tttcaacctt ttcgggcgta taggttccat tgcaaaccga tgtaaccgcc tcgttatagc
    13441 attgtgttac cgtttttaca tattcagggc ctcgggcacg gccttcaatt tttagaactc
    13501 taacgcccgc atcaaggatt ttatccagga agttaatggt gcacaaatcc ttgggcgaca
    13561 ttatatactc attgtcaatc tccagctgtg caccagtttc attatcggtt acggtgtatg
    13621 aacggcgaca aatttgaagg caagctcccc tgtttgccga cgagttggcc tcatgtaagc
    13681 ttaggtaaca ctttcccgat atagccatgc agagcgcccc atgagcaaat agttctattt
    13741 ttatgggatt gccatttggc ccagtaatat tctgcgagtg aatgttttgg taaatagttt
    13801 ttacctgttc aagatttagc tctcgggcta aaacaactac atcagcccac tgtgagtaga
    13861 acttaagtgc atctgtattg ctaatattca actgtgttga aatatgcact tccattcccc
    13921 ttttgcgggc gtaaagcatg gcagcctggt cggagataat aagtgcgctg acacctgctt
    13981 cggcagcggt gtcgataatt tggtgcatga aaggaatgtc gctatcgtaa attaccgtgt
    14041 taacggtaag gtatgttttt accttgttct ttttgcaaat gcgaaccact tccggtaaat
    14101 cgtccacagt aaaatttatt gacgatttag cgcgcatgtt gaggtttcca acaccaaagt
    14161 aaaccgaatt tgctcctccc tgtatggctg ccatgagcga ttcaaaattg cccgctggag
    14221 ccataacctc aatgtcctcg cgttttagca tttgcaaatt ttttgcaaaa ataggaaaat
    14281 tagttagtgt ttaccctaat ttaaaattgt ttagggcatt gtgaatcctt tttcctaaag
    14341 ttgaacccta tttggtttca accaaatatc ccttggggtc aactgggata ccctttatac
    14401 gaacctcata atgaaggtgt ggtccagtgc ttcgaccagt actgccagta taacctataa
    14461 catcgccttt tcttataggg ctccccttgg aaacctttat tttatccaaa tgggcataaa
    14521 ccgaacttgt tccatcatta tgttcaataa tgatatagtt tccgtaggtg ctgttgtact
    14581 gggcaagaac aactttgccg ctcgatgctg cataaacctc agccccacgt cccgagccaa
    14641 aatccacacc attatgttta ataggccggc ctagggttgg gtgaattctt acaccgaaag
    14701 gcgatgtaat tttggctagt cttttgggta aaggtgtaag tactggtatt ccatcgcgag
    14761 tgccagcctc tggtttaaat gttgatgacg acacttcggg ctttttatct tcaacattta
    14821 cattctcagc ttctgtttta gcttcttcct tttttgaaac ttcggactta gtattggctt
    14881 ccactttttc ctttaccctt tcaggaattt tgtatcccaa tgttttagca atcgtgtttt
    14941 cgtaatcaat gatatcctga acggtttgag ccttttcggg ttcctttttc tcaacataat
    15001 gcttcttgag tccagtcatt tcattgtagt agtccaggta ctcctgttcc atttttttta
    15061 gctcagcttc gcgcttttgt ttgaaatcct gaaaatcctg ctcacgctcc ttcttaaatt
    15121 tttcaaaatc ggcttccagc tgacttttgg ttttattctg tgcatttgtg cttattatca
    15181 aaacattaag aagtgcaact aaaaagataa accttttcat ggccgataaa gtttttgttt
    15241 ttataaccat tcaataactt tgccgaaatg gcaaaacaac gacataaaaa tttactgaaa
    15301 gcagataagt gcgttttgaa attggtccaa tcagttgtta ataatacggc aaaaaattga
    15361 ttttagaata attttaacta agtatcttaa gatatgaaac ctacacttgt tattttggct
    15421 gctggcatgg gtagccgtta tggtggtttg aagcaggttg atgcattagg acccaatggc
    15481 gaaacaatca ttgattactc cgtgtacgat gctattcgtg ccggttttgg gaaagttgtt
    15541 tttgtaattc gcaagagcat tgaaaaggat tttttggaag tatttggtaa ccgtttcgac
    15601 tcagatgtaa gagttgaaat tgcctttcag gaactcgata tgcttccgca gggttttaaa
    15661 gttcctgatg gccgcgaaaa gccatgggga acggcacatg cagtatgggt aacccgtcat
    15721 ttaattaatg aaccgtttgc tgtgattaat gccgacgatt tctatggttt cgacaccttt
    15781 agggtactag ctcaggaact atccaaaccc aatctggaaa agggaagcta ctttatggtt
    15841 ggttacaagc ttggaaacac cctttcggaa caaggcgctg tttcacgcgg agtatgctca
    15901 accgataaaa atggatactt attagatgtt gttgaacgca ctcacattga gcgtgttgaa
    15961 ggcgaggtaa agtacaaaga tgaaaatggt aattttgtaa aggttgatga gggcgttccc
    16021 gtatcgatga acttctgggg tttcaccact gactttttcg gctacaccga gaagcttatg
    16081 accgactttt tcagcaacca aattggaaat gttaaggcag aattttacat tcccacggtt
    16141 gtaaaccgag ccattaaaga gggtattgcc cgctgtaagg tattagaaac caaagcggag
    16201 tggtttggcg ttacatatcc cggtgataga cccatggtag ttaaccgact taaagagtta
    16261 acggaaaaag gagtgtatcc atcaccttta tggagtaggt aaaaattagg agtgtgatac
    16321 tattgatgta gctcatttgt gctagatatt ttgctcttag ttatactttt gcttttaacc
    16381 ttaaaccaaa accttactga tatgtcgttt gcaacagtcg attacattgt gtttatagcc
    16441 tatgctttac ttatagttag tttaggcttg tgggtttcgt ttcgtaagcg cgattcggaa
    16501 accaactcag gggattactt tttagccggt agggcgttgc cctggtgggc tattggtgca
    16561 tcgctaatag catcaaatat ctctgctgaa caattcattg ggatgtcagg ttcaggctat
    16621 gccattggat taggaattgc atcgtatgaa tggatggcag ctttaacgct aattctggtg
    16681 gctgttttct ttctgccgat atttttggag aagaggattt ttaccatgcc gcagtttctt
    16741 gaggtccgct acgataaacg tgttaaaacc gtaatggctg gcttttggct gctggttttt
    16801 gttttcgtta atcttacctc tataatctac cttggtgctc tggctatgca gcgaataatg
    16861 ggtataaata tgacctacgc tattattggt ttagcctttt ttgctgcact ctactccatt
    16921 tatggtggct taaaggctgt agcatggacc gatgttgtgc aggttgtttt tcttgtagga
    16981 ggtggtttag taacaacata ctttgcttta gattacctaa gcgatggcca tggctttatt
    17041 gctggattta aagccttact ccaaaaggct cccgaaaaat ttgatatgat tctggaaaaa
    17101 ggcaaactga tgatacccga tgggaagggt ggacaaaagg atgcctatat tgatttaccc
    17161 ggaattagcg tactaattgg cggtatgtgg gttgcaaacc tctactactg gggttgtaac
    17221 cagtatatta ttcagcgtgc cctagctgcc aaaagcatta aggaggcaca gagcggattg
    17281 gtatttgctg cattcctaaa ggttttactt ccacttattg tagttatccc tgggattgtt
    17341 gcctttgcat tacatgccga tattgcaaag tcagacgagg cgtatccttg gctacttcac
    17401 aactttgtcc ctactggtgt aaagggcttg gcttttgctg cattggtagc ggcaattgta
    17461 agctcattgg catcgatgat gaatagtgtt tcaacaatat tcactatgga tttttataaa
    17521 gaatatataa acaaggatgc ctcggaacat aaacttgtta cagttggtcg tatagcagca
    17581 ttcgttgcgt tgctaatagc agtattcata gcacccatgc tttcaaacct cgatcaagct
    17641 tttcagtata ttcaagagtt tacgggattt gtaagtcctg gcgcattagc aattttccta
    17701 gccggatttt tctacaaaaa agcaacagcc aatggtgctt taggagcagc agttggaaca
    17761 ttcgtgttct cgctactatt taagttactt ctacccaacc tgccctttat ggataggatg
    17821 ggaattgttt ttctaatctg tttagcaatt atctggatat ttgctatggt tgaggggaat
    17881 aagcagcatg taaaagctat aacaatttca aaggaactct tctcaacggg taaaggattt
    17941 aagataggct cagcaataat tatagccctt ttgattattc tttacgctat ctggtggtaa
    18001 tttctaataa ttcaaataaa aaagggtacc aattttgggt accctttttc attgcatata
    18061 actagaattt atctagtatt tagctgatga cgatgtgctg aaatacttga catctttacc
    18121 tagaatatcg cttaatagta catcggcaag gcgactagtt ccaaaccgga atagtccagg
    18181 ataaagccac tcctgaccta gaacctgctc aacaatagta tagaaaagcg cagcatcgta
    18241 ggagtttgaa actcctccag caggcttcat acctaccttg cgtccggttt tttcatggaa
    18301 ctccttaatg gctaaacaca ttacgtaaac cgcttcaggg gttgctgcgg gttctaactt
    18361 gccggttgag gttttaatga aatcggaacc agcctccatg gctaaaaacg aagcgagttt
    18421 tatgtcttca aggcttggta atgcgccagt ttcaagaatg actttaacct tggcgttctc
    18481 agccacctct ttaatttgtt gaagttcgct gaaaaccgta aagtaatcac cttcaaggaa
    18541 agaacctacc gatatcacca catcgagctc
//
LOCUS       DAOSAL010000052        18515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_2090_length_18515_cov_52.624594, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000052 DAOSAL010000000
VERSION     DAOSAL010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 18515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18515
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2090_length_18515_cov_52.624594"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            486..614
                     /locus_tag="PK049_07985"
     CDS             486..614
                     /locus_tag="PK049_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61537.1"
                     /translation="MGELPRDTIRHAFGVTHVVSLTKPTTTLHPRPIFGPGFYATA"
     gene            complement(1073..3208)
                     /gene="scpA"
                     /locus_tag="PK049_07990"
     CDS             complement(1073..3208)
                     /gene="scpA"
                     /locus_tag="PK049_07990"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HOK61538.1"
                     /translation="MKPDFKKIDIKHIDKSSGVLNVEADWLTPERIPVKPAFTETDLE
                     NMEHLNYAAGIPPFLRGPYSTMYVMKPWTVRQYAGFSTAEESNAFYRRNLAAGQMGLS
                     VAFDLATHRGYDSDHERVVGDVGKAGVAIDSVEDMKILFDGIPLNKMSVSMTMNGAVL
                     PVMAFYIVAALEQGAKLEELSGTIQNDILKEFMVRNTYIYPPEFSMQIIADIFEYTSR
                     KMPKFNSISISGYHMQEAGATADIELAYTLADGLEYLRTGVKAGIDIDAFAPRLSFFW
                     AVGMNHFMEIAKMRAARLLWAKIVKQFNPKNPKSMALRTHSQTSGWSLTEQDPFNNVA
                     RTCIEAMAAALGHTQSLHTNALDEAIALPTDFSARIARNTQLYIQEETNVCRAVDPWA
                     GSYYVEYLTHEIAHKAWKLIQEVEELGGMAKAIETGIPKMKIEEAAARKQARIDSGKD
                     IIVGVNKYRLDKEDPIEILDVDNTAVREAQIRRLQKLRAERNEAEVQAALNAITRCVE
                     TGEGNLLELSVEAAKKRATLGEISYACEKVVGRYKAVIRSISGVYSAESMDDPNYQKA
                     RELCEKFAKLEGRQPRIMIAKMGQDGHDRGAKVVSTGYADIGFDVDIGPLFQTPAEAA
                     KQAVENDVHVLGVSSLAAGHKTLVPQVIEELKKLGRDDIMVIVGGVIPPQDYQFLYDH
                     GVVGIFGPGTSVASAAVKILEILLGKFEE"
     gene            complement(3241..5124)
                     /locus_tag="PK049_07995"
     CDS             complement(3241..5124)
                     /locus_tag="PK049_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOK61539.1"
                     /translation="MAEKSNEKLFTEFPPVSTAEWEAVIREDLKGADYDKKLVWKTLE
                     GFNVRPYYRSEDLANLETVHVKPGDFPFVRGNHQKGNPWLIRQDFEVCLDKPTEANRK
                     AIDMLSRGVESLGFNLCSDCEPSYDSISRLLKDIDLSRVEVNFTGGSASAKALPFIIK
                     FFEQSGVKPTDIKGSIDYSPLTTFALKGKFCCENDASYQGLVNVLNAVKSFPNFRVIG
                     VNAHVFHNSGSSAVQELAFGLAMASDYLNALTDKGFGIDELAARIRFNFAVGANYFME
                     MAKFRAARMLWAKMVEAYNPKSKDAARMSIHAETSRWNLTVYDPYVNMLRTTTESMSA
                     VLAGVDSLNVLPFDAPFAQPSAFSERIARNQQILLREESHFGKVADPAAGSYYIENLT
                     ISIAEYAWNLFLEVDSQGGFREAFTKGFIQEKINEVAAKRDANIASRRETVLGTNQYP
                     NFTEVADLAKVKPEAVSRPKPVKSADAIAQPLVPYRGAQAFEELRYRTDSSGRRPRVF
                     MLTLGNLAMRRARAQFSCNFFAVAGFEVIDNIGFNTVDDGVKAAMDAKSDIVVICSSD
                     DEYATLAPEAFERLNGKAIFVVAGEPACKPDLEAKGIANYISVKSNLLDTLLKYQEML
                     GIK"
     gene            5574..5801
                     /locus_tag="PK049_08000"
     CDS             5574..5801
                     /locus_tag="PK049_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61540.1"
                     /translation="MRRILKFVFVLASLILLIPAKSEAKFIGAECETVTTGGGDSRYV
                     TKSICKKYFFWIRYDTVVTVESIDCSHLNNQ"
     gene            5872..6516
                     /locus_tag="PK049_08005"
     CDS             5872..6516
                     /locus_tag="PK049_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61541.1"
                     /translation="MNYKVIVSLILLFFNSAIFAQENHFLFDSVIQIQEVTVYAKQKM
                     EVLNNKKSRNVMHIRGYGNASIVSKINLNKDNVYHIKAVEFFFNYKWSGVDNEGFYIK
                     PIIKCVDNGKPSNDLLNSQCVYFVGNNINEEIYINLSHYNIVLENTDSFFVGFELVSA
                     SENAKVEEFNITMVPVKNSQNISYLKGSCSKCDFSALNLDEKNALQLKYKIYYK"
     gene            6763..7701
                     /gene="amrB"
                     /locus_tag="PK049_08010"
     CDS             6763..7701
                     /gene="amrB"
                     /locus_tag="PK049_08010"
                     /inference="COORDINATES: protein motif:HMM:NF013992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOK61542.1"
                     /translation="MSRIMSLQGEMLAKSPTVNGCRLAVCPHDDYTYVGWLYPATLRN
                     ISAKTIILFGVAHKARKFNLEDALVFDSFTHWHGAYGNIPVSELREHIIKELPGDMAI
                     RHDSMHIVEHSVESMLPFLQYSKRDIEIVPILVPCMSFDRMKEVATALATALQKVFQK
                     YDLSWGSDVALVVTTDAVHYGDEEWGGRNYAPFGTDSAGYRKAVDLELEIINTTLKGT
                     LNDVKIRNFFGYTVDAENYREYKWTWCGRYSIPFALLTAIKLNQLMKGSDLIGLPVGY
                     ATSIDHQPLPVADLGMGQTAPANIRHWVGYAAVVYP"
     gene            8314..13254
                     /locus_tag="PK049_08015"
     CDS             8314..13254
                     /locus_tag="PK049_08015"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HOK61543.1"
                     /translation="MELQEIDLKSYRIKFFGIKRTTRSGVVHVGWNSENIGGSSGGTA
                     QFEVPAIDDFSVLSAKVVQSPSQYIQILFSENLSENQDYEGLVDVDGLSNLKFEQDGN
                     ILRVYLPLEKQGASEVKVFKGIKNFKGKKLEHDYTERFVFEDIKPGLRSLGKGTVLPT
                     SQGLIYPFEAVNLKEVTVEVLKIFENNIPYYLQVNRLGENYDLRRVGYSIFKKNISLF
                     DYGVVVPNKWTRFTLDMSKFFEADPGALYQIKISFNKKQLLTPCDKEENSNEATPDDN
                     VTSGNDYYEDYDEYYYSDDYDWNERDNPCNSAYYSSSRMISQIILSSDIGLLAKKGEN
                     DKILVVVTDLPTAKPKSGVTVKVTDYQNQLIVEGQTNSDGIAELQLSRTPYLVTAIDG
                     NQKGYLRVDNGTSNSLSNFDVGGFEVQRGIKGMIYGERGVWRPGDTLHITFILEDKAK
                     SIPENHPIIFELHDPKGLIVKKMVASNSPVGIFYFPVPTDEQAPTGNWRAIVKVGGAV
                     FEKYLKVETIKPNRLKINFELGEIPASGEVNAPIHVKWLHGENAANLEAKYEITLSKG
                     NAVIPKYSQYNFNDPGIDFETNTYTLWEGTLDANGNSRVVGRINKSKSMPAAVNVAFK
                     GRVFEQGGDFSIDLFSKTYYPYNKLVGVLAPKPKPGMGWLETDADQEFSVVTIDAKGN
                     AVNSSQLEVELYKLSWSWWWEQLDNSDANYVSRNYDKLITRKKISTVNGKATFPLRVD
                     YPEWGRFYLKITDKETGVSCGNIVYFDWPWGYSKTQADRPGGASILAIATSKNECSVG
                     ESVQLTIPGAENARALVSIENGTRVISAWWVDASKPTNVIEVKTTPEMSPNVYLHVTL
                     IQPHKGKTNDLPIRLYGISRLVVTDPQTILQPVIQMPDEIKTNQTFDIKVSEKNGKRM
                     SFTLAVVDEGLLDINRFKTPDPHAVFYTQEAIGVKTWDIFNDVIGAYGGKLERHISIG
                     GDDELRSQDGDNNQRFKPVVRYFGPFSLGKNDTKKVSVNLGNYIGSVRVMVVAAYEGA
                     YGNAEKACPVRNDLMVLATLPRVLGPQEEISLPVNIFTMTDKVKNVTVKVKTSSNISI
                     VGDEQRSVSFNKPGDRMVYFNLRVDGTGPAKVTVEASGNEVKSYQTIDIYVRNPISAV
                     TRSIDTIITANGKISALFSTFGIPGSNSAMLEASVMPPLNLKRRLDELIQYPHGCIEQ
                     TTSAVFPQIYLDKLVELSSEQRGNINYFVNESITKLSKLQNTDGSFRYWPSYNYSDEW
                     STSYVGHFLIEAQNAGYNIPQSILNNWVNYQRNAAREYLYDASRYTNSGLIQAYRLYT
                     LALAKKPEIGAMNKLKEQQLDLTAKMRLAAAYALIGQVETAKALIQNTPIKFEPYREL
                     SFTYGSDLRDKAIVLETLVLLNDDSNGFRLAREISNSLSGNRWLSTQETAVSLLALSK
                     MSMQTKERSDVNLRYTINGKTEKITTEKPIARSVLQIKEGNNNITYENLSTKPMFVNL
                     SLTGIPPLGEEKTFESNITMKVAYLSNDGNVISPEQLKKGTDFKCEVTVFNPGVRGEV
                     KELALTQIFPSGWEIQNTRLESGENTTSSNFDYQDIRDDRVITYFSLKPNESKKFSVK
                     LTATYAGKFYMPGPYCEAMYDNTIASYKKGGWVRVE"
     gene            13202..15610
                     /gene="pbpC"
                     /locus_tag="PK049_08020"
     CDS             13202..15610
                     /gene="pbpC"
                     /locus_tag="PK049_08020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HOK61544.1"
                     /translation="MITLLHRIKKAAGLEWNKLIKPLVIIKTAKPFIKYMLAGVILLL
                     AFLLFVKPVSFKASSSTVLLDRNGQLLAATVTDEGMWQFPAPDSVPFRFSKCLLQFED
                     SYFYYHPGFNPVSLVRAAIQNLRNRKIISGASTITMQLVRLSRKGKQRTYMEKAIEIV
                     LAFRTELAYSKKSILAMYASHAPFGGNVIGLDAAAWRYFGTSPDNLSWAEAATLAVLP
                     NAPGLIFPGRNQHLLLAKRNFLLGKLLKKGVIDNQTYQLALLEPLPDPDNKYSQRATH
                     LLTRCIKDGLKGKIIRSTISRELQDIFYKIALRYHRHFKANGIRNMAILVVDNLTGEV
                     LVYIGNILTDDPSFSSKVDIITAKRSYGSLLKPFLYAYMLSEGLLLPKEFVKDVPVAI
                     AGFTPLNFSKKFEGAVKANEIISRSLNVPSVILLKEYGIEKFYNNIHKTGLLSINMPP
                     SHYGLSIILGGAESTMWELARAYSAIARAALSDTLRETSIRYTVDRGMLFNQAFEAAN
                     IKPGAAYLTLQALTEANRPGEDGTWRNFIASQTIAWKTGTSFGFRDAWAIGVTPKFTV
                     SVWVGNADGEGRPDLVGAATAAPVMFDVFSVLPSCSWYSPPYYDLKTVKTCKKTGMLA
                     SNLCPDTTEELVPAVKIKTLPCIYHKKIFLDKSKRYRVNRSCFPNSDLKDTTWFILPP
                     GQEWFYKKNHFDYTVLPPMHPKCSVQYDEINNIDLIYPFYGVDLYIPIENDGTRGQII
                     LKATHRKSESIIYWHLNNEFIGMTKNVHEIAISPKPGSYTLTLVDENGEELKRRIKII
                     GEDR"
     gene            complement(15817..17043)
                     /locus_tag="PK049_08025"
     CDS             complement(15817..17043)
                     /locus_tag="PK049_08025"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOK61545.1"
                     /translation="MNVPLYIARRYLFAKKSHNIINIISVISVIGVATGVMALVVVLS
                     VFNGFDSLIANLYSHIDADLRIEAKQGKTFRTDSLPIERVKQIPGVAVFSEVVEENAL
                     FRYRGKQHIGIVKGVSRNYTELTGLKSKIVDGDFKLWQGSQPMAIMGEGVAYYLNANL
                     AHFDPLFVYIPRRGKNYSPDAAFIKKAIMPSGIFAIEQDFDTRYVITPVEFARGLLLY
                     DSLTVSAIEIKLHTSANAARVKTDVENIIGNKYRVLDRYQQNESLYRTMKSEKFAIAL
                     ILILILIIASFNIVGSLSMLIIDKRADVETLKSLGASNSLIQKIFLFEGMLISIGGSI
                     IGIIFGLITCWLQITFKLIRLEGRGNFIVDAYPVEVQASDMILIFLVVIAIGYLAARF
                     PVRVITRRLIQNDSRN"
     gene            complement(17049..17819)
                     /locus_tag="PK049_08030"
     CDS             complement(17049..17819)
                     /locus_tag="PK049_08030"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOK61546.1"
                     /translation="MISRLVSFTTRFIPRHYLQLFASFLMKAIGLFYRGSRFQDPIDG
                     VKYRKLLPYGRVTTRENALAPNSMSLERHRLIWLYLQRKTDFFTAPKKVLHVAPEYCF
                     IKPIKRLRNIDYTTADLISPWADVKLDVQAMPFADNTFDMVICNHVLEHVDNDLRAMA
                     EILRVLKPGGFAVLQVPMDLNMEQTLENPEYNTPELREKYYQQRDHLRLYGRDYAKRL
                     TSAGFDVLEDNFVKQLPPHEVEYYALPKDEILYIARKK"
     gene            complement(17830..18168)
                     /gene="rbfA"
                     /locus_tag="PK049_08035"
     CDS             complement(17830..18168)
                     /gene="rbfA"
                     /locus_tag="PK049_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOK61547.1"
                     /translation="MEGTRLQKVGRLIQKELSEIFQREGAARFPGKMLTVTQVRVSPD
                     LSVAKVYVSIFPSTDTDKTLEHIRDNSKFLRGELGKRVRHQLRVIPELTFFIDDSLDY
                     IEKIDQLLKE"
BASE COUNT         5503 a         3644 c         4057 g         5311 t
ORIGIN      
        1 gtgtgcagtg ttcagtgtgc agtgttcagt gtgcagtgta cagcagggtg gtgatgttta
       61 aggataaagg ataaaggata aaggataagg ttcaaggttt aaggttcaaa gttcaaggtt
      121 catgtaaaag gcacaatgtc ataggcttag gatttattat caactatcaa ccatcaacta
      181 aataggtatc acagagttac acagtgtaac acggtgttac acagaatggc attcacgaac
      241 aacgaatcac gatcaacgaa caacgaacgc cgtccccagc cccgtagggg cgaaatattt
      301 gtaaccccat ggctactccc acatgtatag cgatgcacgc aatgcctttt ccaaagaaca
      361 acactgaatc attgcatcgc aaaggaaaag catggcaaag agccattcac acgttctttc
      421 ttgtcttgat acaagaaaga accaaagaag atcaaggctg aaaagcccga cccgatgggt
      481 gccccgtggg ggaactgcca cgcgatacaa ttcgccacgc cttcggcgtg actcatgtgg
      541 tatcgcttac taagcccacc accacgctcc acccccgacc cattttcggg ccaggctttt
      601 atgccactgc ttaagtcatt atttgttggt tgaggaattt cattaataaa gggaatgaag
      661 gtacaaaggg atgcttcggc gagctcagca tgaaaatggg agtaaggaaa aaaggggaat
      721 gtcattccga actagtcgag gaatctctct caggtatagc gaacagccct gtaggggcaa
      781 atcaactaag cccagggttt taaccctggg tattgttgta ttcaaaattt tgggcgatgc
      841 acgcaatacc ttttccaaag aacaaccctg aaacgttgca tcgcaacaaa aaacattgca
      901 aagtgcaatt gcaggaaaac aattccccaa ggatacagga cttaaggtat atagagatgc
      961 ttcagcttgc tcagcatgac agaaaggcgt tttagcggag tatatactta taaatcagga
     1021 ataaataaaa aagggttgca atcgcaaccc tttttcagtt taaacgatgt gattactctt
     1081 cgaatttccc gagcaggatt tccagaattt taacggcagc tgaagccact gaggttccgg
     1141 ggccaaatat gcccacaacg ccatggtcgt aaaggaactg gtagtcctga ggaggaatta
     1201 cgcccccaac aataaccatg atatcgtcgc ggccaagttt ctttaattcc tcaattacct
     1261 gaggaactag ggttttgtgg ccggcggcca agctggacac gccaagcaca tgaacatcgt
     1321 tttcaacggc ttgtttagct gcctcagcag gggtttggaa tagaggccca atgtccacat
     1381 caaagcctat atcggcatag ccggtcgata caaccttggc gccacggtcg tggccatcct
     1441 gccccatttt ggcaatcatg atacggggct ggcgtccctc gagcttagcg aacttttcgc
     1501 agagctcacg ggctttctgg tagttaggat catccataga ttctgctgag taaacgcctg
     1561 aaatggaacg gataaccgct ttgtaacggc caaccacttt ctcgcaggca tatgatattt
     1621 caccgagtgt agcacgtttc ttggcagcct caaccgatag ttccagcagg ttcccttcgc
     1681 cggtttctac gcaacgtgta atggcattga gggcagcttg aacctctgcc tcgttgcgtt
     1741 cagcacggag tttttgcagg cgacggatct gcgcttcgcg cacggcggtg ttgtcaacat
     1801 caagtatttc aatgggatct tccttgtcca ggcggtactt gttaacacca acaatgatat
     1861 ccttgcccga gtcaatacgt gcttgcttgc gggctgcggc ttcctctatt ttcatttttg
     1921 gaatgccggt ctcaatggct tttgccattc cgccaagctc ttctacctcc tgaataagtt
     1981 tccatgcctt atgggctatc tcgtgggtaa ggtactcaac gtagtacgaa cctgcccatg
     2041 ggtcaactgc gcggcaaacg ttggtttcct cctgaatgta tagctgagtg ttgcgggcaa
     2101 tacgagccga gaaatcggtt ggaagggcaa tggcctcatc gagtgcgttg gtgtgcaagc
     2161 tttgtgtatg acccagcgct gctgccattg cctcaatgca ggtacgggct acgttgttaa
     2221 agggatcctg ctcggttaag ctccaccccg atgtttggct gtgtgtacgc agcgccatcg
     2281 atttgggatt cttggggttg aactgcttaa caatcttagc ccagagcaag cgtgcagcac
     2341 gcatcttggc tatctccatg aagtggttca tgccaacagc ccagaaaaac gataggcggg
     2401 gcgcaaaggc atctatatca ataccagctt ttaccccagt gcgcaggtac tcaagcccgt
     2461 cggcaagagt gtatgctagc tcaatatcgg cagtagcacc ggcctcctgc atgtgatacc
     2521 ctgaaatgga gatgctgttg aacttgggca tcttccttga ggtatactca aagatatcgg
     2581 caataatctg catggaaaat tcaggagggt aaatgtaggt gttacgcacc atgaactcct
     2641 tcaggatgtc gttctgaatg gttccgctta gctcctcgag cttggcaccc tgctcaagcg
     2701 cagccacaat gtagaatgcc ataacgggga gtactgcgcc gttcatggtc attgaaaccg
     2761 acatcttgtt gaggggaatg ccgtcgaaaa ggattttcat atcctctacc gagtcgatag
     2821 ccacgcctgc cttacccacg tcgccaacta cacgctcgtg gtcggagtcg tagccgcggt
     2881 gagtggctaa gtcgaatgct actgaaagcc ccatctggcc agcggccagg ttgcggcggt
     2941 aaaaggcatt tgattcctca gcggttgaaa agcctgcata ctgtcttaca gtccaaggtt
     3001 tcatcacgta catggtgctg taagggccgc gaaggaaagg cggaatacct gccgcatagt
     3061 tcaggtgctc catgttctcc aggtcggttt cggtaaaggc aggtttcacc ggaatacgtt
     3121 cgggtgttaa ccagtcggcc tctacattta acacaccgct acttttatcg atgtgtttta
     3181 tatctatctt tttaaaatcg ggtttcattg tttacctctt atggttaagt aaaattgcag
     3241 ttacttaatt cccagcattt cctgatactt gagcagggta tcgagcaggt tgctcttcac
     3301 gctgatgtag ttggcaatcc cctttgcctc aaggtcgggt ttgcaggcgg gctcgccggc
     3361 cacaacaaaa attgctttgc cattaagcct ttcaaaggct tcgggggcaa gtgtggcgta
     3421 ctcatcgtcg gagctgcaaa tcaccacaat gtcggactta gcatccattg cagcctttac
     3481 accatcgtca acagtattga aaccgatgtt gtcaataacc tcaaagccag caacggcaaa
     3541 gaagttacag ctgaactggg cacgtgcacg acgcatggcc aaattgccaa gggttagcat
     3601 gaacacacgt gggcgacggc cgctactatc ggttcggtag cgcaactcct caaaagcctg
     3661 agcaccacgg taaggaacaa gaggttgagc aatagcatcg gctgatttca cgggctttgg
     3721 acggctaact gcctcgggtt taaccttggc aaggtcggca acctcggtaa agttggggta
     3781 ctggttggtt ccaagaacgg tctcgcggcg cgaggcaata ttggcgtcgc gtttagcagc
     3841 aacctcatta atcttttcct gaataaatcc ttttgtaaag gcttcgcgga atcctccctg
     3901 gctgtcaacc tcaaggaata ggttccaggc gtactcagca attgagattg taaggttctc
     3961 aatgtagtag ctgcctgcag caggatcggc tactttgcca aagtgcgact cctcgcgaag
     4021 caatatttgc tggttgcggg caatacgctc actgaaagcc gaaggctgag caaatggtgc
     4081 atcgaatggc aacacgttga gcgagtcaac tccagccaat acggcgctca tgctctcggt
     4141 tgtggtgcgg agcatgttaa cgtatgggtc gtaaactgta aggttccaac ggctggtctc
     4201 ggcatgaatg ctcatgcggg cagcatcctt gctcttgggg ttgtaggctt caaccatttt
     4261 tgcccagagc atgcgtgcag cacggaactt agccatttcc atgaagtagt ttgctcccac
     4321 tgcaaagttg aaacggatac gagctgctaa ctcatcgata ccaaaaccct tgtcggttag
     4381 ggcgtttagg taatcgctgg ccatggcaag gccaaaggcc aactcctgaa cagccgagga
     4441 gccactgttg tggaatacat gggcgttaac accaataacc ctgaagtttg ggaaactctt
     4501 tacggcgttt agtacattca ccaaaccctg gtacgaggca tcgttctcgc agcagaattt
     4561 gcctttaagc gcaaaagttg tgagcgggct gtaatcaata gaacctttga tatcggttgg
     4621 ctttacgccc gactgttcaa aaaacttgat aatgaaagga agcgccttgg ctgatgctga
     4681 tccccctgta aagttaacct ccacccttga aaggtcgata tccttaagca atcggctaat
     4741 ggagtcgtat gagggttcac agtcgctaca taggttaaaa cctaacgatt ccacaccgcg
     4801 gctcagcatg tcgatcgctt ttcgattggc ttcggtgggt ttgtcgaggc atacctcaaa
     4861 atcctgacgg atgagccaag gattaccttt ttgatggtta ccccttacaa acgggaaatc
     4921 gcctggttta acatgaactg tttcaaggtt tgcaagatcc tcggagcggt agtatggcct
     4981 aacgttgaac ccctcgagcg ttttccatac caacttttta tcgtagtcag ctcctttcaa
     5041 atcctcgcgg attaccgctt cccactcagc tgttgaaacc ggcggaaatt cggtaaaaag
     5101 cttctcgttg cttttttctg ccataatcag acttttagga tttttgattt gcaaatttaa
     5161 caattgctac aatagcagcc tgttaatttg gggtgataat taagcgaata atgctattaa
     5221 gcataaaaat tggttgcggt agggttaaac aggcttaaca ctgatttgtt attttaaaga
     5281 gtttaagtac gtcttatgat gcttttagtt attgaatagt cattttaagg tatcaaagat
     5341 tctcactaat gcaatcctaa ttgaccatat aatgaagaaa tcgcttagga tgcgtataat
     5401 aatatatgtt aatattacga attgtgttga aaaattaaaa tatgctgtat aacataatat
     5461 aaaatagatt aatctattat tattattatt gtagatgttt ggatttaggc ctaatcaaaa
     5521 actacccaaa acccaagggt atatttgttt gggtttatta aatattaaac tatatgagga
     5581 gaatattaaa atttgtattt gtattggctt cattaatact acttattcca gctaaatcgg
     5641 aagcaaagtt tattggagca gagtgtgaaa ctgtaaccac tggtggaggt gatagtcgct
     5701 acgttactaa aagtatttgt aaaaaatact ttttttggat ccgttatgat actgttgtaa
     5761 ctgttgaaag tattgactgc tcacatctaa ataatcagta attttttacg actggaataa
     5821 tgttcatatt ataccagtca ttttttgttt acatattaaa aaaatactat aatgaattac
     5881 aaagttatcg tatctttaat actattattt tttaattctg ccatttttgc tcaagaaaat
     5941 cattttctat ttgactcagt gattcaaata caagaggtta cggtttatgc caaacaaaaa
     6001 atggaagttc taaataataa aaaaagtaga aacgtaatgc atataagagg ctatggtaat
     6061 gcttcaattg tatccaaaat aaatttaaat aaagataatg tatatcacat caaagcagtt
     6121 gaattctttt ttaattacaa gtggagcggc gtggataatg aggggtttta tattaaacca
     6181 ataattaagt gtgttgataa tggtaagcct agtaatgatt tgttaaactc acaatgtgtt
     6241 tattttgtag gtaataatat taacgaagaa atatatatta atttatcgca ttacaatatc
     6301 gtattggaaa atactgattc tttttttgtg ggatttgagt tagttagtgc ttcagaaaac
     6361 gctaaggttg aagagtttaa catcactatg gttcctgtaa aaaactctca gaatatatca
     6421 tatcttaaag gttcatgttc taaatgtgat ttttcagcac ttaatttgga tgagaaaaat
     6481 gcgctccaac taaagtataa aatatattac aagtagttta tattcaaaaa attttaccaa
     6541 aaacttattt taccaacatt tatctaatat taaataattg attaattgat aaaatattta
     6601 tatttttagg catcaacata ggatatagta atgaacttta ggttaatagt gctattgtca
     6661 ctctttttga caaattgtaa gcacgaatat aagaatgtat cggttcgcaa gccagtcgat
     6721 actattggat ttgctacaaa gcagtatcaa atagattcgg tcatgagccg aattatgtca
     6781 ctacagggcg aaatgcttgc aaagtcccct acagttaatg gctgtaggct tgcagtttgc
     6841 ccacacgacg actataccta tgtgggatgg ctatacccgg ccaccctcag gaacatcagc
     6901 gcaaaaacta ttattttatt tggtgtggca cacaaagccc gcaagtttaa ccttgaggat
     6961 gcgctagttt tcgattcctt cacacattgg catggtgctt acggaaacat tcctgtgtcg
     7021 gagctgcgtg aacacatcat caaagaattg ccaggggata tggctattag gcacgatagc
     7081 atgcatattg ttgaacactc cgttgagtcg atgctacctt tccttcagta tagtaaacgc
     7141 gatatcgaaa ttgtgccaat cctggttcca tgcatgagct ttgataggat gaaggaagta
     7201 gcaacagcgc ttgcaactgc ccttcaaaag gtttttcaga agtatgattt gagttggggt
     7261 tccgatgttg ctttggtagt aacgaccgat gcagtacatt acggtgatga ggagtggggt
     7321 ggcaggaatt atgccccctt tggtaccgat tcagcaggtt acagaaaggc ggttgatctg
     7381 gaactcgaaa ttattaatac cacactgaaa gggacactca acgatgtgaa aatccgtaac
     7441 tttttcggtt atacggtcga tgcagagaat tacagggaat acaaatggac ctggtgcggc
     7501 cggtatagta ttccatttgc gttgcttact gccattaagc tgaatcagct tatgaaagga
     7561 tccgacctca ttggattacc tgttggttac gctaccagta tcgaccacca acccttaccg
     7621 gtagcagatc tgggtatggg tcaaaccgca ccagcgaata tcaggcactg ggtgggctat
     7681 gcagcggtgg tttacccgta gtcacaattg aattacctct aaattttaaa taatatgaga
     7741 actagtaaat ttaatttatt aaaaatccta ttactcgttt taataggtgt ttcaacattg
     7801 atttcatgta agaagaaagc aaaagacatc ataatcgaag ttgatccaac ttttggccaa
     7861 tacatttcgg catatacatc gggcatggtg tcagccaatt caactattac aattcgattt
     7921 acaatgcctg cgcaaaaatt tactaaggca ggtgacgaat tagacgataa tgtatttaaa
     7981 atttcaccca gcatagatgg gaaaacctat tgggtggatg atcaaacaat cgaattcaaa
     8041 cctactaatc ctttagtatc tggcgaaata tatacaataa ggttttttct tggcaaagta
     8101 ctagaagttc atgatagcag gtttgaaaag tttgattttt cgatacgagt aattcctcaa
     8161 tctattgacg tagattttga tggtttaata attaacggga aggatgactc tattacctat
     8221 aatctagagg gcactgtgag ggtggcggat tatacttcca ttgaaaaatt gacaaaatgc
     8281 ttttgggcaa attataatag tcaaaattta gagttggaac ttcaggaaat tgatctgaaa
     8341 tcctacagaa tcaaattttt tggcataaag aggacaaccc gttcgggtgt tgtacatgta
     8401 ggttggaatt ctgagaacat tggtggaagt tccggtggca ctgcccaatt tgaggtacct
     8461 gctattgatg acttttctgt tcttagtgca aaagtagttc aatctcccag tcaatacatt
     8521 cagattctat tttcggaaaa tctgagtgag aatcaggatt atgaaggttt agtagatgtt
     8581 gatgggttgt caaatctgaa atttgagcag gatgggaata tactgagagt ttatcttcca
     8641 ttagaaaaac agggtgctag cgaagtgaaa gtttttaaag gcattaagaa ttttaaaggg
     8701 aaaaagttag agcacgatta cactgagcgt tttgtttttg aagatataaa acccggatta
     8761 cggtcactgg gtaaaggaac tgtacttccg acatcgcaag gtttaattta tccttttgaa
     8821 gcggttaacc ttaaagaggt caccgttgaa gttcttaaaa ttttcgaaaa caacattcct
     8881 tactacttgc aggtcaatcg attaggagag aattacgatt tgcgaagggt gggttacagt
     8941 atttttaaaa agaatatctc attgtttgat tatggagttg tggtccctaa taaatggact
     9001 cgattcacac ttgacatgag caaatttttt gaagcggatc ctggggcttt gtatcaaatt
     9061 aaaatctctt tcaataaaaa gcaactttta acaccatgcg acaaggaaga gaattccaat
     9121 gaagcaactc ctgatgataa cgtaacttcg ggaaatgact attacgagga ttatgatgaa
     9181 tattattaca gtgatgatta tgactggaat gagcgcgata acccatgtaa ttctgcttac
     9241 tactcaagtt caagaatgat aagccaaatt atactgtcat ccgatatagg tttgctagct
     9301 aaaaagggcg agaacgataa gatacttgtt gttgtcaccg acctgcctac agcaaaacca
     9361 aaatcgggtg taactgtaaa agttactgat taccaaaacc agttaattgt tgaaggacaa
     9421 acgaattctg atggtattgc tgaattacaa ctctcacgca caccctatct tgtaactgcc
     9481 atagatggaa accaaaaggg atacttaaga gttgataacg gaacgtcgaa ctcgttgagt
     9541 aactttgatg taggagggtt tgaggttcag cgaggaataa agggaatgat ttatggtgag
     9601 cgtggggtat ggcgtccagg cgacaccctt catattacat ttatccttga ggataaagca
     9661 aaatccattc ctgaaaatca tcccataatt tttgaacttc acgaccccaa agggttgatt
     9721 gttaaaaaaa tggttgcatc taatagccct gtaggtatct tttatttccc agttccaacc
     9781 gatgaacaag cacctacagg taattggagg gctattgtaa aagttggtgg cgctgtattt
     9841 gaaaagtatt taaaggttga aaccatcaag cccaataggc tgaaaataaa ttttgaattg
     9901 ggtgaaattc cagcctcagg cgaagttaat gcccctattc atgtaaaatg gttacatggc
     9961 gaaaatgcag ctaaccttga ggccaaatat gagataaccc tttcaaaggg gaatgcagta
    10021 attccaaaat atagccagta taattttaac gatcctggta ttgattttga aactaatacc
    10081 tatactcttt gggagggcac gttagatgcc aacggaaact ctcgagttgt aggtagaatc
    10141 aataaatcga agagcatgcc tgcagccgta aatgttgcat tcaaagggcg tgtatttgaa
    10201 caaggaggcg attttagcat cgatttattc tcaaaaacat actaccccta caacaagctt
    10261 gtgggtgttt tggctccaaa acccaaacca ggaatggggt ggctcgaaac tgatgctgac
    10321 caggaatttt cagtggttac tatcgatgca aaaggaaatg cagttaatag ttcacaactt
    10381 gaagttgaac tttacaagct ttcctggagc tggtggtggg agcaactcga caatagtgat
    10441 gcaaattatg taagcaggaa ctatgataag ttgattacca gaaaaaaaat atcaacagtt
    10501 aacggcaagg caactttccc acttcgggta gattacccag aatgggggcg tttctatctt
    10561 aaaattaccg ataaggagac aggagtaagt tgtggcaata ttgtttactt tgattggcct
    10621 tggggatata gtaaaactca agccgataga cccggaggtg cttctatatt agctatagca
    10681 accagtaaga atgaatgtag tgtaggtgag agtgtgcaat tgactattcc cggtgcagag
    10741 aacgctcgtg ctttagtaag tattgaaaat ggcactagag ttatttcagc ttggtgggtt
    10801 gatgcttcaa aacctactaa tgttattgag gttaaaacaa cccctgaaat gtcaccaaat
    10861 gtttacctgc acgtaaccct tattcagccc cataaaggta aaaccaacga tttacccata
    10921 agactctacg gaatttctag acttgttgtt accgatccgc aaactattct tcaacctgta
    10981 attcaaatgc ctgatgagat aaaaacgaat caaacctttg atattaaagt atctgaaaag
    11041 aacgggaaaa gaatgagttt tactcttgca gtggttgatg aaggcttgct cgatataaat
    11101 cgttttaaaa cacctgatcc gcatgcggtt ttttatactc aggaagcaat aggggtaaaa
    11161 acatgggata tttttaacga tgtaattgga gcctacggag gaaagcttga aaggcatatt
    11221 tccataggcg gcgacgatga actcaggtct caggatggtg ataataatca gcgattcaaa
    11281 cctgttgtac gatattttgg cccctttagt ttaggtaaaa acgacactaa aaaagtttcg
    11341 gttaaccttg gtaactacat tggaagcgtt agggtaatgg ttgtagctgc atatgagggt
    11401 gcatacggaa atgccgaaaa agcatgtccg gtaagaaacg atttaatggt tttggcaact
    11461 cttccaaggg tattaggacc gcaagaggaa atcagtcttc cggtaaacat tttcacaatg
    11521 acagacaagg tgaaaaatgt tacggtcaag gtgaaaacat cgtcaaacat tagcattgta
    11581 ggcgatgaac aaagatcggt aagttttaac aaaccaggag atagaatggt ttactttaac
    11641 cttagagttg atggaactgg acctgcaaaa gtaactgtgg aggcttctgg gaacgaggta
    11701 aagtcatatc aaactattga tatttatgtt cgaaatccta tatcggcagt aactcgaagc
    11761 attgatacaa taattactgc caatgggaaa atttcagctt tattcagcac atttggtatt
    11821 cctggtagta actcagctat gctagaagca tcggtaatgc cacctttaaa cctaaagcgt
    11881 agactcgatg aacttatcca atacccacac ggctgtattg aacaaactac gtctgctgta
    11941 ttcccccaga tatatctgga taaacttgtt gagctctcat cggagcaaag aggtaatata
    12001 aattactttg tcaatgaatc gataaccaaa ctttcaaaac ttcaaaatac tgatgggagt
    12061 tttcgctatt ggccatcgta caactatagt gacgagtgga gcaccagcta cgttggtcat
    12121 tttctgattg aagcacaaaa tgctggttat aatattccgc aatcgatttt aaacaattgg
    12181 gttaactatc aacgaaatgc tgcacgtgaa tacctatatg acgctagccg ttacactaac
    12241 tctggcttga ttcaagctta taggctttat acattagctc ttgctaaaaa gcctgaaatt
    12301 ggagcaatga ataagctaaa ggaacaacag ttggacttaa ctgcaaaaat gagacttgct
    12361 gccgcttacg ccttaattgg tcaggttgaa acggcaaaag cacttattca aaatacacct
    12421 ataaagtttg agccttatag ggaacttagt ttcacctatg gttcggattt acgagataag
    12481 gcaatagttc ttgaaacttt agttctactt aatgatgatt ccaacggttt ccgattagct
    12541 agagaaataa gtaacagtct ttcaggtaac cgttggttaa gcacacagga aacggcagta
    12601 tcactacttg ccttatcaaa gatgtccatg cagactaaag agagaagcga tgtaaacctt
    12661 cggtatacca taaatggaaa aaccgagaaa attactaccg aaaaaccaat tgctaggagc
    12721 gtattgcaaa taaaggaagg taataacaat attacatatg agaatttatc tactaagcca
    12781 atgtttgtaa atctttcact tactggtatt cctcctttag gtgaagaaaa aacattcgaa
    12841 agtaatatta ccatgaaggt agcatacctt tccaacgatg gtaatgtaat cagcccagag
    12901 caattaaaaa aaggtaccga ttttaaatgt gaggtaacag ttttcaatcc tggtgttaga
    12961 ggagaagtta aggagctagc actaactcaa atcttcccat cggggtggga aattcaaaat
    13021 actcgattag aaagcggcga gaatactacg tcgagtaact ttgattatca agatattcgt
    13081 gacgatagag taattacata ctttagctta aaacctaacg aaagcaagaa attttctgta
    13141 aaacttactg caacctatgc cggaaaattt tacatgcctg ggccgtactg tgaagcaatg
    13201 tatgataaca ctattgcatc gtataaaaaa ggcggctggg ttagagtgga ataaactgat
    13261 taagccattg gtgattatta aaacagccaa gccttttata aaatatatgc ttgcaggagt
    13321 aattctcctg cttgcatttt tattgtttgt aaaaccggtt agttttaagg cttcgtcctc
    13381 aactgttttg cttgaccgga atggacagtt gcttgcagcc acagtaaccg atgagggaat
    13441 gtggcagttc cctgcgcccg atagtgttcc cttccgattt tccaaatgtc ttttacaatt
    13501 tgaggactca tacttttact atcatcccgg ttttaatccg gtttcgttag ttagagctgc
    13561 tattcaaaat ttaagaaatc gtaaaataat cagtggtgcg agtacaatca ctatgcaact
    13621 tgttcggcta tcaagaaagg gtaaacaacg aacttacatg gaaaaagcca ttgagatagt
    13681 tttagctttc cgaacagaac ttgcatactc aaaaaaaagc attttggcca tgtatgcatc
    13741 gcatgctcct tttgggggta acgttattgg ccttgatgcc gctgcttggc gatactttgg
    13801 tacaagtccg gacaatttga gttgggctga agctgcaaca ctcgcggttc ttcctaacgc
    13861 ccccggtcta attttcccgg gcaggaatca acatttactc ctagctaaaa ggaactttct
    13921 tctaggaaaa cttttaaaaa agggggttat cgataaccaa acatatcaac tggcactgtt
    13981 ggagccattg ccagatcctg ataacaaata tagccagaga gcaacccact tgcttacacg
    14041 ttgtattaaa gatggattaa aggggaaaat tattcgttct actattagtc gtgaacttca
    14101 ggatattttt tacaaaatag ccctgcgcta tcatcgtcat tttaaggcaa atggcataag
    14161 aaatatggca attttggttg tggataattt aaccggagaa gttctagtat acattggtaa
    14221 tattttgaca gacgatccaa gcttttcatc taaagtagat atcataacag ctaaacgaag
    14281 ttatggtagt ttacttaaac ctttccttta tgcatatatg ctttccgagg gtcttttatt
    14341 acctaaggaa tttgttaaag atgtaccggt agctattgct ggatttacac ctttaaattt
    14401 tagtaaaaaa tttgagggtg cggttaaggc taatgagata atttctcgct cattaaatgt
    14461 tccctcagtt attctcctga aagaatatgg aatcgaaaaa ttctacaata acattcataa
    14521 gaccggttta cttagtatta acatgcctcc ttcgcattac ggtttgtcaa taatacttgg
    14581 aggtgcagag tccactatgt gggaattggc tcgtgcatac tccgcaattg caagggctgc
    14641 attaagcgat actttgcgtg aaaccagtat ccgttatacg gttgatagag gaatgctttt
    14701 taaccaagca tttgaagctg ctaatattaa acccggtgca gcgtatctta cgcttcaagc
    14761 attaacagag gcaaatagac ccggggagga tggcacatgg cgtaatttta ttgcttcgca
    14821 aactatagct tggaaaaccg gaacaagttt tggtttcagg gatgcttggg caattggtgt
    14881 aaccccaaaa tttacagttt cagtttgggt tggaaatgcc gatggcgaag gccgacccga
    14941 tttagttggt gcagcaacag cggctcctgt aatgtttgat gttttttctg ttttaccttc
    15001 gtgtagctgg tattcgccac cttactatga tctaaaaacg gtaaaaacat gcaaaaaaac
    15061 gggtatgctt gcttcaaacc tttgccctga tactacagaa gagttggttc cagcagtaaa
    15121 aattaaaaca ttaccatgca tttatcacaa aaaaattttt cttgataaat ctaaacggta
    15181 tagagtaaat cgttcctgtt ttccaaatag tgatttaaaa gatacaactt ggttcatcct
    15241 tcctccagga caggagtggt tttacaaaaa aaatcatttt gattatactg ttctcccccc
    15301 aatgcaccct aagtgtagtg ttcaatatga tgaaataaac aatattgacc tcatatatcc
    15361 attttatgga gttgatcttt atattccaat agaaaatgat ggcactcgtg gtcaaataat
    15421 tttaaaagca acacatagaa agtctgaatc aatcatttac tggcatttga ataatgagtt
    15481 tataggtatg actaagaatg ttcatgaaat agccatcagt ccaaaacctg gaagctatac
    15541 tctaacactt gtcgatgaaa atggtgagga attaaagcgt agaataaaga tcattggaga
    15601 agataggtaa tctttcaaaa aattaggatt atttattagt tttaattatc tggtaatttg
    15661 ttatggctta atttgtttat tgaatttatt ttttttgatt caatttcaaa taatatgaac
    15721 aaacatcatc ctgttaggat gaacttacat tttggtttaa aacaaagaaa aatcaaaatg
    15781 gaaagtatac ttgaaaaatt aataaaaaaa gcgtttctaa ttcctactat cgttctgaat
    15841 caacctccgc gttataaccc taaccggaaa tcgggctgcc aggtatccta tggctataac
    15901 caccaggaaa attaggatca tatcgctggc ctggacttca acggggtatg catcaactat
    15961 aaagttgcca cggccctcaa gcctaatgag cttaaaagtg atttgcagcc aacatgttat
    16021 caaaccaaat ataattccaa ttatactgcc accaatggaa ataagcatgc cctcaaagag
    16081 aaatatcttt tgtatcaggc tattgcttgc tccaagactt ttcagagtct ccacatcggc
    16141 gcgtttatca ataattagca tggagagcga tcccactata ttgaatgatg caataatgag
    16201 tataagtatc agtattagcg caatggcaaa cttttccgat ttcatggttc ggtaaagcga
    16261 ctcgttttgc tggtaacggt ccaataccct atacttattg ccaataatgt tttcaacatc
    16321 ggttttaacc ctggcggcat ttgcacttgt gtgcagcttg atttcaatgg cgctaacagt
    16381 tagtgaatcg taaaggagta gaccccttgc aaactcaacg ggtgtaatta cataccgggt
    16441 atcaaaatct tgctcaatgg caaaaatgcc cgagggcata atggcttttt taataaaggc
    16501 agcatcgggg gagtagtttt tgccccgcct gggaatgtaa acaaacagcg ggtcaaagtg
    16561 ggctagattg gcgttgaggt agtaggcaac cccttcgccc atgatggcca tgggctggct
    16621 accttgccat agcttgaaat cgccatcaac tattttgctc tttagtccgg ttagctcagt
    16681 gtagttcctg ctaacccctt taacaattcc aatgtgttgc ttcccgcggt agcggaaaag
    16741 ggcattctcc tcaaccactt cggaaaatac tgccacaccg ggaatctgct tgaccctttc
    16801 aatgggtaag ctatcggttc ggaatgtttt tccctgctta gcttcaatcc gcaaatcggc
    16861 atcgatatgg gagtagaggt tggcaatcag ggaatcgaag ccattgaaaa cggatagaac
    16921 aaccacaagc gccataaccc ctgttgcaac cccaataacc gatattacgg agatgatgtt
    16981 gatgatgtta tgcgattttt tcgcaaacag gtaacgtcgg gctatgtaaa gcggaacatt
    17041 cattggtttt acttttttcg ggcaatgtaa aggatttcgt ccttaggtag ggcgtaatat
    17101 tccacctcgt ggggtggcag ctgcttaaca aagttgtcct ctaaaacatc aaacccggca
    17161 gaggtaaggc gtttggcgta atcgcggccg taaaggcgga gatggtcgcg ctgctggtaa
    17221 tacttttccc tgagttcagg ggtgttatac tccgggtttt caagggtttg ctccatgttt
    17281 aaatccattg gtacctggag cacagcaaac ccacccggct ttagcacccg gagtatttcg
    17341 gccatcgccc taaggtcgtt atcaacgtgt tcaagcacat ggttgcaaat caccatatca
    17401 aaggtattgt cggcaaaagg cattgcctga acatcgagct ttacatcggc ccagggcgaa
    17461 atgaggtcgg ctgtggtata gtcaatgttt cttagtcgct ttatcggttt tatgaaacag
    17521 tattccgggg caacatgcag aacctttttg ggggctgtga aaaagtcggt tttcctttgc
    17581 aggtaaagcc atatcagccg gtgccgttcc agcgacatgg agtttggcgc cagtgcgttt
    17641 tcccgtgtgg taacccgccc gtaggggagt agcttgcgat acttaacccc atcgatagga
    17701 tcctgaaacc gactgccccg gtaaaaaagt ccaatggctt tcatcagaaa cgatgcaaaa
    17761 agttgaaggt agtgccgggg tatgaaccgg gtggtaaacg aaactaaacg acttatcatt
    17821 cttgggtttc tactctttaa gtaactggtc aattttttcg atatagtcga gcgaatcgtc
    17881 gatgaaaaag gtaagctcgg gtatcaccct aagctggtgc cttacccttt tgcccagttc
    17941 gcccctaagg aattttgagt tatccctgat atgctcgagc gttttatcgg tatcggttga
    18001 ggggaatata ctaacgtaaa ccttagccac cgagaggtcg gggctaaccc taacctgcgt
    18061 aactgtaagc attttaccgg gaaacctggc agccccctcg cgctggaaaa tctcgctaag
    18121 ctccttctgt atgagccgtc ccaccttctg taatcgtgtt ccttccattt ccgtattttt
    18181 tacaaagata taacgatttt atgaggtttg ggtaagcgga acaatgattg gtgtaatact
    18241 tgtattttca attgcttgct gcagtttgat ttttcgtacc gctagttaac tatcccactt
    18301 gtcatcctga gcgtagcgaa aaacagtgtg cagtgtgcag tgtacagggg agattgtgtt
    18361 tggtttttgg tgtttagtgt ttagttaatt agaaagctgt gtaaattcaa taaaataagt
    18421 gtaatctgcg ttctatgaaa ctcactatca atcataaacg aacaacttca agtgttaggt
    18481 aaagtggttt acaaaaaatt ataagtccat ttgat
//
LOCUS       DAOSAL010000053        17266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_2278_length_17266_cov_49.112777, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000053 DAOSAL010000000
VERSION     DAOSAL010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 17266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17266
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2278_length_17266_cov_49.112777"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            228..1478
                     /locus_tag="PK049_08040"
     CDS             228..1478
                     /locus_tag="PK049_08040"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOK61548.1"
                     /translation="MNRFFLLILSLNLLVVISGCKKPKRYDLIVENATISTDSGLVDG
                     KTILVMGDTIAGIIDAGEPVRTRKVVDADGGIVMPGFIDSHTSIACFFKSNGKAFDTH
                     PKSLTTFYRSIVSKHFLPYGVTTIFDAEPDSAWVPQIVSWKPNPKLTDVMVARSVEMG
                     KSACNPSQSPYVFIKGIYDSKNQEHAGDNCFWILTGVQNGLNYPKNIEGIQSIMNSLL
                     LLTGSSDKVLNRIANIYGIRQNIPDELLAIEGISYLAENSPVLLDSVAAILGSNNASV
                     STGLYRIKHFVDSGFTTCGIEPTEAIKQRLKYGFAHLMEYVKALHTKGVELRIGYNMP
                     FDGSAFAKEQNLLLESGFPVNEIGKISSFNSAKAFAIHGKKGKIAIGYTADLVVFLKN
                     DAGNSFDFTKVKRVIKGGRAEKMK"
     gene            1536..2306
                     /locus_tag="PK049_08045"
     CDS             1536..2306
                     /locus_tag="PK049_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61549.1"
                     /translation="MMHKEFIYHPRDFRKIELWKNVKDEEWYNPQWQLKNSIRSVDQL
                     KHVIKLNDYQEQEIRRVVDELERQGKDPLRITPYYASLMDEDPFHPDIPLEEKGNKRL
                     DPIFWQSVPTPANLLFPNTGREASMNEGSRSYGAAYQRYPNRVALFVAQNTSCASYCV
                     HCQRAKSLDATCDVNIELINKGLFYINFNKNIDEVLVTGGDALRVSKKRLQYVLEELS
                     KMEHIRAIRIATRVPVVLPMAVTDEILTLIKESANRYT"
     gene            2331..3065
                     /locus_tag="PK049_08050"
     CDS             2331..3065
                     /locus_tag="PK049_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61550.1"
                     /translation="MTHVNHYKEITADFAAAIKRINSYGFTVRNQTVFLNHVNDYFVT
                     LAKTFRRMFWVGVHPYYLLQCHKEKGIVHFISPIQVGQIYMKHMQGWLSGIVRPNYAV
                     NIEGGGGKVLLMPLNYDNLNIDLKIEDKISESKAKVYTWDDKEIFDYEALGRATPDEY
                     NKALRIMDKFIGRKGVFVPKIILIDNNGNYIETTNRTKLPTFEKQKKAVLLGYETNQS
                     GMPITNPAKISKKLDELYQKSTFSKK"
     gene            3147..4328
                     /locus_tag="PK049_08055"
     CDS             3147..4328
                     /locus_tag="PK049_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOK61551.1"
                     /translation="MKNNILNHNLIRLYILKVSHWFMLVMPIVVLFYKENGLNVSQVF
                     ILQSVYSLSIVLLEIPSGYFADALGRKNTIFIGAVLGFIGFAIYSISYGFWGFLMAEV
                     VLGFGQSMISGADSALLYDSLVDAKQQDRYIKHEGRMTSIGNFAEASAGILGGLLAAV
                     SIRYPYYGQTLVALLAVPAAFTLIEPKTEHNRLELGWKQIVDVVRFALVKNLRLRWNI
                     LLSSVVGASTLTMAWFVQPWLIRADTPVEAYGAIWTVLNLLVGVAAMFAYRVELKLGR
                     NRTTGVLILFLFLGFVMAGFIRSYWAMPFIVIFYLARGVATPILKDSINRIAPAPMRA
                     TILSVRNFIIRIIFSVWGPFYGWLTDRWSLKVALITAGFIFIILSTLTFIALFKVERE
                     N"
     gene            complement(4325..5086)
                     /locus_tag="PK049_08060"
     CDS             complement(4325..5086)
                     /locus_tag="PK049_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOK61552.1"
                     /translation="MKITFLGTGTSQGMPVIACPCPVCQSTDPRDWRLRTSALIEHQG
                     INIAIDAGPDFRQQMLRAKVKTLQAILLTHEHRDHISGLDDVRAYNWLQKRPMDIWGE
                     ARVLNELKREYSYVFAEHKYPGIPEFELHSIDGQPFEVMGINIIPIRVYHYKLPIYGF
                     RFGDLTYITDANFIPEDEKEKIIGSKYLVINALRTQKHISHFSLPEALELIAQLSPRK
                     AFITHIGHQMGFHNEVAKTLPENVCLAYDGLELEF"
     gene            complement(5087..6004)
                     /locus_tag="PK049_08065"
     CDS             complement(5087..6004)
                     /locus_tag="PK049_08065"
                     /inference="COORDINATES: protein motif:HMM:NF013885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61553.1"
                     /translation="MQMFRYLKLIVLNRNAILVFAVIAGIIVGEHAGSLKFLTFPALA
                     LTMTFSMTGMSLVQLRSEPHIFKPMLTGIALNYVAFSAVMLPLAYWLMPTKELFYGFV
                     IIAATPPGVAIIPFSFILRGNVVYAIISVTGAFLGSILIAPLMVKLFATSSGINPTDL
                     LLLMLQLVVAPLVVSRFLLWKPIKPFIEKVRGKVVDWGFAILIFVAVGLNRQVFFSSP
                     ALLLSITGILVVTIFGLGHFTNILARHLNISQEKRIPMVMLIGIKSSGFSVFTALTLF
                     GKEAAIPSAVMAVVVLLYLIFLSIKSKAL"
     gene            6052..6768
                     /locus_tag="PK049_08070"
     CDS             6052..6768
                     /locus_tag="PK049_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018694880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOK61554.1"
                     /translation="MAEVKGKLYLIPTPISGSEPENVLPQRVIDITRHLSYFVVEELR
                     TARRYLSRIKVQKPIDELVFFELNEHSQPEAVEAMLTPLLQGYDVGLMSEAGVPAIAD
                     PGAILVAAAHRKGIRVIPLVGPSSILLTLVASGLNGQNFAFVGYLPVKPDERRKRIRM
                     LEQRSRSEGQTQLFIEAPYRNIQLLKDLISVCSEQTTITIATEVTSTDEFIVTKTVRE
                     WKNIPLPDINKKNTVFALLA"
     gene            complement(6771..7763)
                     /locus_tag="PK049_08075"
     CDS             complement(6771..7763)
                     /locus_tag="PK049_08075"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOK61555.1"
                     /translation="MNRNLLLIIAGLSLSAISCDKDFKDPEGDEMARFNAWIQVNNIP
                     ADAKKPSGLYFVSQAEGNGLTPQKGDWILYSYTEKTLDGLVWFTDNSELIKQYDRFDI
                     NYHYTPVFVKYEKAPVQITGYSRTLLKGVYEGLGYLKEGGKATLYIPYNLGYGTSSAT
                     NGLGYQSLIYDIELHKVIKDPRAYEKSLINDYVAQNYPGLEPINDSIYYIQLTPPTVD
                     TVTLAKDSIAYVYYKGMFLDGFVFDTNIDSVAQKLGRKFSSTDSLKVTVGGNDVIKGF
                     SQALLGMKKGEWGRAIIPSFCGYDSIGNSNIPPFTPLIFDIYFSSKGKASTKPK"
     gene            complement(7815..8711)
                     /locus_tag="PK049_08080"
     CDS             complement(7815..8711)
                     /locus_tag="PK049_08080"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOK61556.1"
                     /translation="MRLTHFFLFSSFLAIVASCDNSLETDKRYAEEKSIESYISSKKW
                     SYTKSNGVYCSPQSQSYGYEVNYGDTILFWYKGYTITSPILVFDSNIKSVAIEAKLDT
                     MVRKFEPLRAVIGSTPLLDGLKYGLLLCRENQKATILFPSNLGFGDSYVGPVMPWSPL
                     AFDVEVIYLNGPGKVNEQNLFRTFNLSEYTLHSSGLYYQQVVDTGIVRPTETDKVFGS
                     YTVKLTGGQEIESFATTTEPITLSNLTPHAVRIGFTLASVGGTVNIIAPSPLAYGKKG
                     TPKVSPYTPVEITVKLDSIKTN"
     gene            complement(8708..9598)
                     /locus_tag="PK049_08085"
     CDS             complement(8708..9598)
                     /locus_tag="PK049_08085"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOK61557.1"
                     /translation="MAMNRLFVPAVMALLTLAACSNKSKLFPGYSVTDSGIHYKLITL
                     GESDTPASISNYVTAVIAYRTINDSLFFQGVRQFQLTAPEYEGSIDECFLMLNTGDSA
                     SFYIQAEPFFTKTLGTSLPRFINPGEFMRVDIKLLEVKTAEEFQKEMEAFMSWINDFG
                     EYEKVILKQYLDGQKVDAQPTESGLYIIHQVKTQRSSVEIGDTVTIHYEGRFLNGKLF
                     DSTRKRGEPFQFVYGQKWQVIPGLEEAIGKMREGEKAMLIVPSHLAFGQQGNSNGMIP
                     AFTSVIFELEILEVKKGLAQ"
     gene            complement(9660..10190)
                     /locus_tag="PK049_08090"
     CDS             complement(9660..10190)
                     /locus_tag="PK049_08090"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOK61558.1"
                     /translation="MVNKINTLLIFLLLLSSCNPSTQNVAEQPKSIPDTKQKLLGINY
                     QLTEKDRVLIKAYIDRHRLTMKENPSGLFVYVYGTGYGEYPKKGDLVNYYYRISLLDG
                     TECYKSEPGKPKSMVVGHGGVEAGLEEGIMLMRKGQKALFILPPHLAHGLVGDMDKIP
                     PRASIVYEVELVDVQH"
     gene            complement(10194..11216)
                     /locus_tag="PK049_08095"
     CDS             complement(10194..11216)
                     /locus_tag="PK049_08095"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOK61559.1"
                     /translation="MLQIDKSQLNELKKALSSSKNIVITTHHNPDGDAIGSVLGLYHA
                     FRAKGIDSRMVTPNGFPDFLGWLPDADKIMHYSRDKKDVEDLLSNADLLFCLDFNGFK
                     RVEDMAEVLEKSPAKRVLIDHHPEPESPFHISISYTEVSSTAELVYEVIEQIYGESII
                     NYESAVSLYTGIMTDTGSFSYACSRERTFAIAGKLIAKGVVVEDVQGLVYNNFSENRM
                     RLLGFSLAERMKVYPEHRAACIWLSRNDLNTFKHQIGDTEGFVNYPLSIKGIVFSVLF
                     TENEGFVKVSLRSRGNFPVNTFSKLYYQGGGHKNAAGGKAFMGMDEAVKQFEELIAKH
                     SIELNS"
     gene            11307..11726
                     /gene="ndk"
                     /locus_tag="PK049_08100"
     CDS             11307..11726
                     /gene="ndk"
                     /locus_tag="PK049_08100"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: protein motif:HMM:NF001908.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOK61560.1"
                     /translation="MNTRFTFCMIKPDAFKSDTVSDILLDILKAGFKIRGIKITMLTK
                     SKAEQFYDIHQGKEFFDRLTTFMSSGPVMAMVLERPDAVESLRKLIGKTDPAQADEGT
                     IRKKFGSDTTHNAVHAADSYENAEREWSFFFSKREIY"
     gene            complement(12071..12144)
                     /locus_tag="PK049_08105"
     tRNA            complement(12071..12144)
                     /locus_tag="PK049_08105"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12108..12110),aa:Arg,seq:acg)
     gene            complement(12230..12598)
                     /gene="rplS"
                     /locus_tag="PK049_08110"
     CDS             complement(12230..12598)
                     /gene="rplS"
                     /locus_tag="PK049_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOK61561.1"
                     /translation="MDLIKIAEQAFVEKKNHPDFKSGDTVVVHYKIKEGNKERIQNFR
                     GVVIQRKGTGATETFTVRKISGGVGVERIFPLHSPFIEQIEVAKEGRVRRARIYYLRN
                     LTGKKARIKEKKRGVEGENM"
     gene            complement(12772..13863)
                     /gene="hydE"
                     /locus_tag="PK049_08115"
     CDS             complement(12772..13863)
                     /gene="hydE"
                     /locus_tag="PK049_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HOK61562.1"
                     /translation="MIIDNLLNKDNLNKEELVYLLGCEGEEKTKLFTKAAEVKAKYIG
                     RKVYFRGLIEMSNICRKNCLYCGIRSGNTNVHRYNLTDDEVIEAAKFAWENRYGSIAI
                     QTGEVSTKEFVDRIENLLDKINKATNHELGITLSLGEQTEETYRRWFNKGAIRYLLRI
                     EASNRELYAKLHPNDKHHDFDERLECLRALRRVGYQVGTGVMIGLPFQTLEHLADDLL
                     WMQEIDVDMVGMGPYVEHPDTPLYQYRDKLLPLTERFNLALKMTALLRILMKDINIAA
                     PTALQAIDPLGREKAIKVGANIVMPNITPGAYRNDYKLYENKPCTDDAADDCKNCLEA
                     RIQLTGDEIGYGEHGNSRHFYRRRNDTTI"
     gene            13945..14637
                     /locus_tag="PK049_08120"
     CDS             13945..14637
                     /locus_tag="PK049_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61563.1"
                     /translation="MERAKAIGQIIAVIKQLKKETDELFAPENIINHSELLIEEYFQQ
                     LFNPFLKKVKAEIRSLPLDEYLPIELDSWIRILKMKLVAIERGEYTEKYAEVLYIKQR
                     FYPELIDVLKELQLEALEAEFDARVDREQLSKPILGPNTIVLSIESVAMLIAMLYNFN
                     IISGAVPIDDICRSFAAMTGYNAGELKQLLRFDEENGRLTSIVKAEDMDVLLKLLRSI
                     VARIENLRQYTD"
     gene            complement(14634..15029)
                     /locus_tag="PK049_08125"
     CDS             complement(14634..15029)
                     /locus_tag="PK049_08125"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOK61564.1"
                     /translation="MKALRIEAEIDSPEIYFNPDTKVFSISGISHPENAKEFYQQILD
                     WLDEYYEFIKNQEPSKIIVDLNFRYINSTSYKYLRDVLRKISSFRSANFEVEVIWNYH
                     EEDEELLHEGEVLFELPDINLPYKCIPYN"
     gene            complement(15350..15721)
                     /locus_tag="PK049_08130"
     CDS             complement(15350..15721)
                     /locus_tag="PK049_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HOK61565.1"
                     /translation="MTRGLITILLLVLSNTFMTLAWYGHLKFKDMKWFENLSLVGIVL
                     ISWGIALFEYFFQVPANRLGFRENGGPFSLVELKVIQEAITLVVFMGFTLFAFKTETI
                     RTNHIIGFGFLILAVYFIFKK"
     gene            15873..16751
                     /locus_tag="PK049_08135"
     CDS             15873..16751
                     /locus_tag="PK049_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOK61566.1"
                     /translation="MLVSMTGYGKAERVFGNKKIVVEVRSLNSKQFDFNLRLPAIYRE
                     LEMEIRTKAAKELERGKVDMSISIEETAESQAIPINDSVFMAYYRQLSDIASKNSISF
                     NPAEVVPAILRLPEVIRAQKEEIAPEELDAVLQACAEALNALIQFRKQEGAILEKDLL
                     SRVNLILDYLQRVEPFEKQRIHDVRKRISDSLKQIDANIKVDDNRFEQEVIYYLEKLD
                     ITEEKVRLRQHCSYFTETVNQPESVGRKLGFIAQEMGREINTLGSKANNAEIQKIVVM
                     MKDELEKIKEQSLNIL"
     gene            16755..>17266
                     /gene="gmk"
                     /locus_tag="PK049_08140"
     CDS             16755..>17266
                     /gene="gmk"
                     /locus_tag="PK049_08140"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOK61567.1"
                     /translation="MEGKLLIFSAPSGAGKTTIVRHLLEKFPQLEFSVSACSRMPRDG
                     EVNGKDYYFLTVDEFRNRIEKGEFVEWEEVYPGSYYGTLWSEVKRIWDKGHHVMFDVD
                     VKGGVNLKRKFPNNSLAVFVMPPSIDELRERLLKRGTETEESLKVRIGKAQEEMTYAN
                     QFDYVLTNNQV"
BASE COUNT         4955 a         3456 c         3712 g         5143 t
ORIGIN      
        1 cagagggtgg taacaacgat ggtatggttc agtatctgaa attccgtggt aaagcgccat
       61 caattgatcc gcttttacga aaccggggat ttaagtagaa tatgaatagg ggttgcaaac
      121 gcaaccccta attttttttc tttaagcgtt tattcttata tttacaaggt gtttaaagtg
      181 tttgcccgtt tatcagtgtt tctaacccat gtaatctttg taaagctatg aatcgatttt
      241 ttttacttat tcttagcctt aaccttttag ttgttataag cggttgtaaa aagcccaaac
      301 gatacgattt gattgtggaa aacgccacca tttctaccga tagcggtttg gttgatggaa
      361 aaaccatttt agtgatgggt gataccattg ctggaataat agatgccggc gagcctgttc
      421 gcacccgcaa ggtggttgat gccgatggtg gcatagttat gcccggtttt attgatagcc
      481 atacatcaat tgcctgtttt ttcaagtcga acggcaaagc ttttgatact caccctaaaa
      541 gcctgacaac cttttaccga agcattgtat ccaagcattt tttaccctat ggggtaacca
      601 ctatttttga tgctgaaccc gattccgctt gggttcctca aatcgtaagc tggaagccaa
      661 acccaaaact taccgatgtt atggttgcac ggtcggttga aatgggaaaa tctgcatgta
      721 atcccagcca atcaccctac gtttttatta agggtattta cgatagcaaa aaccaggaac
      781 acgcggggga taattgcttt tggattctta ctggagttca aaatggtttg aactatccta
      841 aaaatatcga ggggatacaa tcaattatga attcgttact cctgttaact ggctcatccg
      901 ataaagtgtt aaataggatt gcgaatattt atggcattag gcaaaatata cccgatgagc
      961 tgttggccat tgaggggata tcgtatttgg ctgaaaatag ccctgtcttg ctcgattccg
     1021 ttgccgctat tcttgggtca aacaatgctt cggtttccac agggctttac cggataaaac
     1081 attttgtaga ttcgggcttt accacttgcg gtattgagcc aaccgaagca attaagcaac
     1141 gcttaaagta tggatttgct caccttatgg aatatgtaaa agccctgcat acaaaggggg
     1201 ttgagctacg cattggctat aacatgccat ttgacggctc agcctttgca aaggaacaga
     1261 accttctact agagtcgggt tttccagtta acgaaattgg taaaatatca tcgtttaatt
     1321 ctgctaaggc ttttgccatt catggcaaaa aaggtaaaat agccataggc tatactgccg
     1381 acctggtggt attcctaaaa aatgacgctg gaaatagttt cgattttact aaggtaaaga
     1441 gagtaattaa aggcggaaga gctgaaaaaa tgaagtaagg gtaatttcca attgtgattg
     1501 acttaagtat aattaacaat aaatttaaac aaacaatgat gcacaaggaa ttcatttacc
     1561 acccaagaga ttttcgaaaa atagaacttt ggaagaatgt taaagatgag gagtggtaca
     1621 atcctcaatg gcagctaaaa aattcaatcc ggagtgttga tcagctaaaa catgtcatta
     1681 aattaaacga ttatcaggag caagagatac gacgtgttgt tgatgagctg gaacgccagg
     1741 ggaaggatcc gctccgaatt actccttact acgcttcgct aatggatgaa gatccatttc
     1801 atcccgatat tcctttagag gaaaaaggaa acaaaaggct cgatccaatt ttttggcaaa
     1861 gtgttcctac accagccaat cttttatttc caaataccgg gagagaggca tcaatgaatg
     1921 aggggtcaag aagttacggt gcagcctatc agcgttaccc caatagagtg gcactttttg
     1981 ttgcgcaaaa cacaagttgc gcatcctatt gtgttcattg ccaaagggca aaatcgcttg
     2041 atgctacgtg tgatgtgaat attgaactta ttaacaaggg cttattttat atcaacttca
     2101 ataaaaatat tgatgaggta ttggttactg gtggcgatgc tttgcgggta agcaagaaac
     2161 gcttgcaata cgttttagag gaattgagta aaatggaaca tattcgtgcc ataagaattg
     2221 ctacaagagt tccagtggtt ttacctatgg ctgtaactga cgagattcta acattaatta
     2281 aagaatcggc caatcgttac acatagggtc cggagaaata cgtttacttc atgacccatg
     2341 taaatcacta taaagaaatt actgctgact ttgcagcagc cattaagcga ataaatagtt
     2401 atggctttac tgttcgtaac caaactgttt tcttgaatca tgttaacgac tattttgtta
     2461 cccttgccaa aacctttcgc cgaatgtttt gggttggggt tcatccatat tacctgctac
     2521 agtgccataa agagaaaggg attgttcatt ttatatcacc catacaggtt ggtcaaattt
     2581 atatgaaaca catgcaggga tggttatcgg gtattgttag accaaattat gctgtaaata
     2641 ttgaaggagg aggcggtaaa gtcctattaa tgcccttaaa ctatgataat ctaaatattg
     2701 acctgaaaat tgaagataaa atttcggaga gtaaagcaaa ggtttacacc tgggatgata
     2761 aggaaatatt tgattacgag gcactaggac gtgcaacacc cgatgagtat aacaaagcat
     2821 taagaattat ggataagttt attggtagga agggggtatt tgtacctaaa ataattctca
     2881 ttgataacaa tggaaattac atagaaacaa ccaaccgcac taaacttcca acatttgaaa
     2941 agcaaaaaaa ggctgtactg cttggttatg agactaacca atcaggtatg cctataacaa
     3001 atcctgcaaa gattagtaaa aagctcgatg agctttatca aaaatctact ttttcaaaaa
     3061 aataaatctg atagtttcct ccgcactata ggtcatgctg aattttcagc atggcctctt
     3121 ttctttatat ttgcattttt actgcaatga agaataacat attaaaccat aatctaatcc
     3181 gcctttacat tctcaaggtc tcccattggt ttatgctggt tatgcccata gttgttttat
     3241 tttacaagga gaatgggctt aacgtaagtc aggtatttat ccttcaaagt gtttattcac
     3301 tttccattgt attgctcgaa atcccatcgg gttactttgc cgatgcacta gggcgaaaaa
     3361 acaccatttt cattggggcg gttttgggct ttattggctt tgcaatctat tcaatttcct
     3421 atggtttttg gggattctta atggctgagg ttgtgcttgg ttttgggcaa agtatgattt
     3481 cgggtgccga ttcggctctg ctttacgaca gccttgttga cgctaagcag caagataggt
     3541 atataaagca cgaaggtagg atgacctcaa taggtaattt tgctgaagct tcagcaggta
     3601 ttcttggcgg attgcttgcg gccgttagca tacgttaccc atactacggt caaacccttg
     3661 ttgccctttt agctgttccg gctgccttta cgcttattga acccaaaacc gagcataacc
     3721 gattggaact gggctggaag caaatagttg atgttgtaag gtttgctctc gttaagaact
     3781 tgcgcttacg ctggaacata ttgctttcat cggtggtggg agcatcaact ttaaccatgg
     3841 cctggtttgt tcaaccgtgg ctgatacgtg ctgatacgcc agtggaggcc tacggtgcca
     3901 tttggacagt gctaaatctg ctagtgggtg ttgctgccat gtttgcctat agggttgaac
     3961 tcaaattggg aaggaataga accactggag ttttaattct ttttctattt cttggctttg
     4021 taatggcggg ttttattagg agttactggg caatgccatt tattgtgatt ttctacctgg
     4081 ctaggggagt agctacccct atactaaaag atagcattaa cagaattgct cctgctccaa
     4141 tgagggcaac aatactatcg gttcgaaatt ttattattcg gataatattt tcggtttggg
     4201 ggccctttta cggttggctt accgataggt ggagtttaaa agtagcctta ataaccgcag
     4261 gatttatctt tattatcctc tcaaccctta catttattgc acttttcaaa gttgaaaggg
     4321 aaaattaaaa ctctagctca agcccatcgt aggcaaggca tacattttcg ggtaatgttt
     4381 tcgctacttc gttgtgaaat cccatttgat ggccaatgtg tgtgatgaaa gcttttcggg
     4441 ggctgagctg agcaatcaat tcaagagcct caggcagtga aaagtgcgag atgtgttttt
     4501 gcgtccttag tgcatttatt accaaatact ttgagccaat aatcttttcc ttctcatcct
     4561 cgggaataaa gtttgcatcg gtaatgtatg ttaaatcgcc aaatctaaac ccatatatgg
     4621 gtaacttata gtggtaaacc cttattggga tgatgttaat gcccatcacc tcaaatggtt
     4681 gaccatcgat gctatgcagt tcaaattcag ggattcctgg gtatttatgc tcagcaaaaa
     4741 catagctata ttcccttttc agctcattaa gcacccttgc ttcgccccaa atatccatgg
     4801 ggcgtttctg aagccagttg taggccctaa catcgtcaag gccggaaata tggtcgcggt
     4861 gctcatgggt aagcagtatg gcctgaaggg tttttacctt ggcccggagc atttgctgcc
     4921 ggaaatcggg gccagcatca attgctatgt ttattccttg atgttcaatg agtgcagatg
     4981 ttctaagtcg ccagtcgcgt gggtcggttg attggcaaac cggacatggg catgcaataa
     5041 cgggcatacc ttgcgaagtt cctgtgccaa gaaaagttat cttcacctac aatgcttttg
     5101 atttgattga caaaaatatc aaatagagca aaacaaccac tgccatcaca gcggaaggta
     5161 ttgccgcttc tttaccaaaa agggtaaggg cagtgaaaac cgaaaaacca gagctcttaa
     5221 tcccaataag cattaccatt ggtattcttt tttcttgaga gatattcagg tgtcgtgcca
     5281 ggatattggt aaaatgccca agtccaaaga tagtaacaac caaaatacct gtaatgctaa
     5341 gtagtagagc aggggaagaa aagaaaacct gcctgtttaa ccctacagcc acaaaaatta
     5401 gtattgcaaa cccccagtcc acaactttac ctctaacctt ttcaataaat ggcttaatgg
     5461 gtttccaaag caaaaagcgt gacactacca acggagcaac cacaagctga agcatcagca
     5521 aaagtaagtc agtagggttg attcccgatg aggtagcaaa gagttttacc attaaaggtg
     5581 caattaggat agaacccaga aatgctcctg taaccgaaat aatggcgtaa accacgttac
     5641 ctctcagaat aaatgagaaa gggattatgg ctacacccgg aggggtggct gcaatgatta
     5701 caaatccgta aaaaagttct ttggtaggca tcagccagta tgccagtggt agcataacag
     5761 ctgagaatgc aacgtaattt aaagcgatac cggttagcat tggtttgaat atatgtggtt
     5821 cgctccttag ctgcacaagg ctcatgcccg tcatggagaa tgtcatggtt aatgctagtg
     5881 cagggaatgt cagaaacttc aaggaacctg catgctcacc cacaataatc cctgcaatta
     5941 cagcaaaaac caatattgca ttccggttca atactataag ctttagatat cggaacattt
     6001 gcattacctt cgtaaaaaat tttagtaaat atagatagaa ggattgaaaa aatggctgaa
     6061 gttaaaggaa aactatacct aattcctaca cccatcagcg gtagcgaacc cgaaaatgtt
     6121 ttaccccagc gtgttattga cataaccagg cacttaagtt actttgtggt ggaggagcta
     6181 agaactgcac gccggtattt gtcgcgtata aaggttcaaa aaccgattga tgagcttgtc
     6241 ttttttgagc tgaatgagca ttcccagccc gaggcggttg aggccatgct tacaccctta
     6301 ctacagggtt acgatgtagg gcttatgtca gaagcaggtg ttcccgctat tgccgatcct
     6361 ggtgctatac ttgttgctgc tgctcaccgt aagggaatac gagtaattcc tcttgttggg
     6421 ccaagttcaa tcctgctaac tctagtagca agcggcctta acggacaaaa ctttgccttt
     6481 gtgggctatc tcccagtaaa acccgacgaa cgacgcaagc gaatcaggat gcttgaacag
     6541 cgctcgcggt ctgaggggca aacacagctt ttcattgagg ctccttaccg taatattcag
     6601 ctcctaaagg atttaattag tgtttgcagt gaacaaacaa ccatcactat agctacggag
     6661 gtaacatcaa ctgatgaatt tattgttacc aaaacagtaa gggagtggaa aaatatccca
     6721 ctccccgata tcaataagaa aaacactgta tttgctttac tggcttaagg ctatttgggc
     6781 ttagttgatg ctttgccctt gctgctgaaa taaatgtcaa agataagtgg tgtgaaaggc
     6841 ggtatgttac tgttgcctat ggaatcgtaa ccgcaaaacg atgggattat tgctcgtccc
     6901 cattcgcctt ttttcatgcc taaaagcgct tggctaaacc cttttataac atcattgcct
     6961 cccacagtaa cttttagcga gtcggttgag ctaaactttc tgccaagctt ttgggccacg
     7021 ctatcaatgt tggtgtcaaa aacaaatcca tcgagaaaca ttcccttgta gtaaacatag
     7081 gcaatagaat cctttgcaag tgttaccgta tcaacggtag gcggggttaa ctgaatatag
     7141 tagatgctat cgttaatggg ttcaagtcca gggtaattct gtgctacata atcattaatc
     7201 aaactttttt cgtatgctct tggatctttg attaccttat ggagttcaat atcgtaaatt
     7261 agcgactggt agcccaatcc gttggttgca cttgaggttc cgtagccaag gttatacgga
     7321 atgtaaaggg tggctttgcc tccttccttt agataaccca acccctcata gacccctttg
     7381 agtagtgttc tgctgtatcc ggttatttgc acgggcgctt tttcatactt tacaaataca
     7441 ggtgtgtaat gatagttgat atcaaacctg tcgtattgtt ttataagctc actgttatcg
     7501 gtaaaccaaa ctaatccatc aagtgttttt tcagtatagg agtacagaat ccaatcgcct
     7561 ttttgcggcg taaggccatt accttcggct tggcttacaa agtataaacc acttggtttt
     7621 ttagcatcgg cggggatatt gttaacctga atccatgcat taaaacgagc catttcatca
     7681 ccttcggggt ctttaaaatc cttatcgcac gaaatggctg aaagtgaaag gcctgcaata
     7741 atcaaaagca aatttctatt catagtttct attcaattta ctgcaaatat actatttacc
     7801 aattcaattc aaagttaatt ggttttaata ctatcaagtt taacagttat ttccaccggg
     7861 gtgtagggtg aaacttttgg ggttcctttt ttcccatacg ctaaaggcga tggtgcaata
     7921 atatttactg ttccacccac cgatgccagg gtaaacccta tacgaacagc atgaggagtt
     7981 aggttgctga gcgtaattgg ctcggttgtg gtagcaaaac tttcaatttc ttgtccgcca
     8041 gttagtttaa ctgtatagct accaaacacc ttgtcggttt ctgttggtcg gactataccg
     8101 gtgtcaacca cttgctggta gtagagtcca ctggagtgaa gcgtatattc tgaaagattg
     8161 aaagttctaa atagattttg ctcgtttacc tttccggggc cattcaagta gataacctca
     8221 acatcgaaag ctaatggtga ccatggcatt accgggccaa catagctatc gccaaatccg
     8281 aggttcgatg gaaaaaggat tgttgccttt tggttttccc tgcaaagtaa caagccgtac
     8341 ttcaagccat cgagtagggg agttgagcct ataacagccc ttagcggttc aaacttgcga
     8401 accatggtat caagcttagc ttcaatggca accgatttaa tattggagtc aaaaactaaa
     8461 atgggtgaag taatggtgta gcccttatac caaaaaagaa tggtatcgcc atagtttact
     8521 tcgtaaccat acgattgtga ttggggtgag cagtaaaccc cattactttt tgtgtaactc
     8581 cattttttac tgcttatgta gctttcgata cttttttcct cagcgtatcg tttatcggtt
     8641 tccagtgagt tgtcgcacga agccacgatg gccaaaaagc tcgaaaaaag aaaaaaatgc
     8701 gttaatctca ttgtgctaat cctttcttaa cctccagtat ttcaagttca aaaatcaccg
     8761 atgtaaatgc gggtatcata ccgtttgaat taccctgttg cccaaaagca aggtgcgatg
     8821 gcacaataag catggccttt tcgccctcgc gcattttacc aatggcttcc tccaggcccg
     8881 gaatcacttg ccatttttgg ccgtaaacaa actggaatgg ctcgccccgc ttgcgggtgg
     8941 agtcgaaaag ttttccgttt aggaaacgtc cctcgtagtg tattgttacg gtatcgccaa
     9001 tttcaacgct actgcgttga gtttttactt gatgaattat gtaaagcccc gattcagttg
     9061 gctgtgcatc aactttttgg ccatcgaggt attgctttag tataaccttt tcgtattcgc
     9121 caaagtcgtt tatccagctc atgaatgctt ccatctcttt ttggaactcc tcggcagttt
     9181 taacctcaag tagtttgatg tcaaccctca tgaattcacc agggttgata aaccttggaa
     9241 gcgatgttcc aagggttttg gtaaagaatg gctcagcttg aatgtagaac gatgcactat
     9301 cgccagtatt tagcatcaga aagcattcgt caatggagcc ttcatactca ggcgcggtaa
     9361 gctgaaactg acgtacaccc tggaagaaaa gggagtcgtt tatggtgcgg tatgcaatta
     9421 ctgctgttac gtaattactt atcgatgcag gggtgtcgct ttcgccaagc gtaatgagct
     9481 tgtaatgaat tccactatcg gttacgctgt aaccggggaa tagtttggat ttatttgaac
     9541 atgccgcaag ggttaaaagt gccataacgg cggggacaaa taagcgattc attgccatgg
     9601 agtgcagggt tttagttttg tttaaggtta taaatttcat tgtaaaaata gaaaatattt
     9661 caatgctgaa catcaaccag ttcaacctca taaactattg aagcccgggg ggggatttta
     9721 tccatatcgc ctaccaaacc atgtgctagg tgtggtggta gaataaaaag cgctttttgc
     9781 cctttccgca tgagcataat accctcctcc agtcctgctt caactcctcc atgccccact
     9841 accatgcttt taggtttgcc cggttctgat ttgtagcatt ctgtcccgtc cagaagcgaa
     9901 atgcggtagt agtaattgac caaatcacct tttttggggt attcaccgta acccgtacca
     9961 taaacatata caaatagtcc gcttgggttt tctttcatgg ttaacctatg cctatcgata
    10021 taggctttta ttagcaccct atccttctcg gtaagctgat agttgatacc caaaagcttt
    10081 tgcttggtgt ctggaattga ttttggctgt tccgcaacat tttgtgttga aggattgcag
    10141 gagctcagca aaagcaaaaa aatcaaaagg gtatttattt tatttaccat acactatgag
    10201 ttaagctcta tggaatgctt ggcaattagc tcctcaaact gtttcacagc ctcatccata
    10261 cccatgaatg cttttccacc ggcagcattt ttgtgtcctc caccctggta gtatagcttt
    10321 gagaaagtgt ttacagggaa attacccctt gaacgcaacg aaactttcac gaaaccttcg
    10381 ttttcagtga aaagaactga aaaaacgatg cctttaattg ataatggata gttaacaaaa
    10441 ccctcggtat cgccaatctg atgtttgaac gtgtttaggt cgtttcggct aagccaaatg
    10501 caagctgcac gatgctcagg gtaaaccttc atgcgttcgg caagagagaa gccaagtagg
    10561 cgcattcggt tttccgaaaa gttattgtaa accaaacctt gaacatcctc aacaactact
    10621 cctttggcta ttagctttcc tgcaatggca aatgtccgct cgcgcgagca tgcgtagctg
    10681 aacgaacctg tatcggtcat aataccagtg tagaggctta ctgcagattc atagttaata
    10741 atactctcgc cataaatttg ctcaataacc tcataaacca gctcggcggt tgagctgacc
    10801 tcagtgtatg aaattgatat atggaaaggg ctttcgggtt cagggtgatg gtcgataaga
    10861 acccgtttgg cgggcgattt ttccaatact tcggccatat cctctacccg cttgaatccg
    10921 ttaaagtcca agcagaatag caggtcggca tttgaaagca aatcctctac atccttttta
    10981 tcccttgagt agtgcataat cttatcggca tcgggcagcc aacctaaaaa gtcgggaaaa
    11041 ccattgggtg taaccatacg tgagtcgata ccctttgccc taaatgcatg gtataggcca
    11101 agaactgagc caatagcatc gccatcgggg ttgtgatgtg tggttattac tatgttttta
    11161 gaactgctta gcgccttttt cagctcgtta agttgacttt tatctatttg aagcatccgt
    11221 taggttattt ttataaactg tttacaaaga tatgttttga gttttacttt acatttacta
    11281 ttttgggaaa ataaattaac agccatatga atacacggtt tactttttgt atgataaaac
    11341 ccgacgcctt taaaagcgac acggttagcg atatcttgct cgatatcctt aaagctggct
    11401 ttaagatccg agggataaag attaccatgc tcaccaagtc aaaggctgag cagttttacg
    11461 atatacacca gggaaaggag ttttttgata ggcttacaac ctttatgagt tcagggcctg
    11521 taatggcaat ggttttggaa cgccccgatg cggttgaatc gctgcggaag ctgattggta
    11581 aaaccgaccc agcccaggcc gatgagggaa caatccggaa aaagttcggg tccgacacaa
    11641 cccataatgc cgttcatgca gccgatagtt acgaaaatgc cgagcgcgag tggagtttct
    11701 tcttctccaa gagagaaatc tattagcgag aatagttaat catttaacgc caacattaaa
    11761 agtgtaactg ctcacttgtt gcataaaaac acgaacaact aacaaccatc aactaaaaaa
    11821 gccagtctgt attgactggc tttcctaagt gaccccggag ggattagttc cgcttcgctt
    11881 catgatccct ccgggctaat gggttaatgt ttgtttttat aaaagcaaga aagccaatcg
    11941 gtaaggattg gctttcttaa gtgaccccgg agggattagt tccgcttcgc ttcatgatcc
    12001 ctccggcaat gcttgcaagg gtttctttac caaataaaga aagccagtct gtattgactg
    12061 gcttatcaag tgaccccgga gggattcaaa cccccaacct tctgatccgt agtcagatgc
    12121 tctattcagt tgagctacgg agtcgtttgt tttgcgtgtg caaagataga tataattttt
    12181 ttttcctgca aagggtggtc aaaaaaaaaa tccccaaacg gggatttttt tacatatttt
    12241 cgccttcaac gccacgtttc ttttctttga tgcgtgcttt tttaccggta aggttacgca
    12301 ggtagtaaat acgagcccta cgcactctac cttccttggc aacttcaatt tgttcaatga
    12361 aaggtgagtg taaagggaaa atcctttcta cgccaacacc ccctgaaatt ttacgaacgg
    12421 taaaggtttc agtagcgccg gttcccttac gctgaattac aacacccctg aagttctgaa
    12481 tacgctcctt gttaccttcc ttaatcttgt agtgaacaac cactgtgtcg ccactcttga
    12541 aatcggggtg attctttttc tcaacaaatg cttgttcggc aatcttaatg agatccattt
    12601 tatactgttt tatttgatta acactaaggt gtggaatatt accggctcct aagtcctgat
    12661 aagagattac acaccaattc ggccgcaaaa ttatttactt tttgggaaac taccaaaaat
    12721 agaagcaatt aattgaaaaa ttttaggtaa aatccaattg gattgtcctt actatatagt
    12781 cgtatcgttt cttctccggt aaaagtgtct tgagttccca tgctcaccat atccaatttc
    12841 atcgccggtt agctgaatac gtgcttcgag gcaattcttg caatcatcgg cagcatcgtc
    12901 ggtgcagggt ttgttctcgt atagcttgta atcgttacgg taggcaccgg gagttatgtt
    12961 tggcataacg atattagctc ccacttttat ggccttttct ctaccaagag ggtcaatagc
    13021 ctgaagtgcg gttggcgcag caatgttgat atccttcatg aggattcgaa gcaaggctgt
    13081 cattttaaga gccaggttaa agcgttcggt aagcggaaga agtttatctc gatactggta
    13141 aagtggggta tcggggtgtt caacgtaagg gcccatgcca accatgtcca catctatttc
    13201 ctgcatccaa agcaggtcgt cggcaaggtg ctctagggtt tgaaaaggca gcccaatcat
    13261 tacccctgtt ccaacctgat atcctacacg tcggagggcc ctcaggcatt ccagccgttc
    13321 gtcaaaatcg tgatgcttat cgttgggatg aagctttgca tagagttcac ggttcgaggc
    13381 ctcaatgcgg agcaggtaac gaatggctcc tttgttaaac catcgccggt atgtttcctc
    13441 ggtctgctca ccaagcgaaa gtgtaattcc cagctcatgg tttgtggctt tgttgatttt
    13501 atccagtagg ttttctattc tatcaacaaa ctctttagtg cttacctcgc cagtttgaat
    13561 ggcaatgctt ccgtaacggt tttcccaggc aaatttggct gcctcaatta cctcatcgtc
    13621 agttaaatta taccgatgaa cgttggtatt gccagaacgg atgccgcagt acaaacagtt
    13681 tttacggcaa atattcgaca tttcaattag acctctgaag taaaccttgc gtccaatata
    13741 cttagccttt acctcagcag cctttgtgaa aagctttgtt ttttcttcgc cctcgcagcc
    13801 caagaggtat acgagttctt ccttatttaa attatcttta tttaaaagat tatcgataat
    13861 cattgtatat tagtttgtat tttttcctaa gtttaccaac taaaatggtg gttggtaatc
    13921 ctttgcaaaa ataatttaat tcccatggaa cgagctaagg caataggtca aataatagct
    13981 gttataaaac agctcaaaaa ggaaacggat gagctatttg cacctgagaa tattattaac
    14041 cattcggaat tgctcattga ggagtatttc cagcaacttt tcaatccgtt tttaaaaaag
    14101 gttaaagccg aaatccgttc tttgccgttg gatgagtatt tacccattga gctggactca
    14161 tggattagaa tactaaaaat gaaactagtt gccattgagc gcggtgagta caccgagaag
    14221 tatgccgagg tgctatatat caagcaaagg ttttacccgg agctaattga tgttttaaag
    14281 gagttgcagc ttgaggcact tgaagccgaa tttgatgctc gtgttgatag ggagcaacta
    14341 tcaaagccaa ttcttggccc taacaccata gttctctcca ttgaatcggt agctatgctt
    14401 attgccatgc tatacaactt caatatcatt tcgggtgcgg ttcccattga tgatatttgc
    14461 aggagttttg ccgcgatgac agggtataat gcaggcgaac tgaaacagct gcttaggttt
    14521 gatgaggaga atggccgatt aacatcaata gtaaaagccg aggatatgga tgtgctgctt
    14581 aagcttttgc ggagtattgt ggcaagaatc gagaacttac gtcagtatac cgattagtta
    14641 taggggatac atttgtaggg caggttgata tcgggtagtt caaacagaac ttcaccctca
    14701 tggagcagtt cctcatcctc ctcatggtag ttccatataa cctcaacttc aaagtttgcg
    14761 cttcgaaacg acgatatttt tcttaaaacg tcgcggagat acttgtatga agtagagttg
    14821 atatacctaa agttaaggtc aacaatgatt tttgatggct cttggttttt gatgaactca
    14881 tagtattcat cgagccaatc gagtatttgc tggtaaaatt cttttgcatt ctccgggtgc
    14941 gaaatgccag aaatggaaaa tactttggtg tcagggttga agtatatttc gggtgaatca
    15001 atctcagcct ctattcttaa agctttcatt gctccttttt caattaacaa cgtaaaatta
    15061 aacctttacc gttattaata gcaaagattt aacaatatct ttgatgaaaa taaaaaataa
    15121 atgggtgaag ctataaacct tttgggtaaa atatcatcaa taataaaaca ctttttaact
    15181 atttgatggt agttaaaatt aattttggtt ttatatttgt agtatacaca ttagcttccg
    15241 gcactttcat ggagagttgg ttagttagtt caaggaagtt ttcgattttc gggtaaaatc
    15301 ggagtgtttg gggcgaagga ggtcaacttg acctccttca tttcattaat tacttcttga
    15361 aaataaagta aaccgctagt atcagaaacc caaatcctat tatgtggttt gtgcgaattg
    15421 tctctgtttt aaaagcaaaa agggtaaagc ccataaatac taccagtgta atagcctcct
    15481 gaatcacctt aagctcaaca agcgaaaagg ggccaccatt ctctctgaaa cctaaacgat
    15541 tagcgggaac ctgaaaaaag tactcgaata gggctatgcc ccagctaata agaactatcc
    15601 ccactaagct taagttctca aaccatttca tatccttgaa cttcaggtgc ccataccatg
    15661 ctaatgtcat aaatgtgttt gacagcacga gtagcagtat ggtaatcaag cctctagtca
    15721 tacgatttat actataaaat tgtatgcaaa attataggtt gttttaaaat gcattgagta
    15781 taaactcttt ttatcctata tttttaagct aattcttgtt aatagtctat caatttttcg
    15841 atacttttgt ggtgatattt aaatatactt gcatgctagt atcgatgacc ggctatggaa
    15901 aagccgaaag ggtttttgga aacaaaaaaa ttgttgttga ggttcgttca cttaactcaa
    15961 agcagttcga ttttaacctc cgcttgccag caatttaccg tgagctggag atggagatca
    16021 gaactaaagc cgccaaggaa ttagagcgcg gaaaagttga tatgtccatt agcattgagg
    16081 agactgccga gtcgcaggcc atacccataa acgattccgt ttttatggct tactaccggc
    16141 agctaagcga tattgcttca aaaaatagca ttagcttcaa ccctgccgag gttgttccgg
    16201 ccattctccg attgcctgag gttattaggg cacagaagga agagattgcc cccgaagagc
    16261 ttgacgcagt tttgcaggca tgcgctgaag cgttgaatgc cttaattcaa ttccgaaagc
    16321 aggagggcgc aattcttgaa aaggatttac tgagccgtgt taacctgata cttgattact
    16381 tgcaaagggt tgaacctttt gaaaagcagc gcattcacga tgttcgtaaa cggatttcgg
    16441 atagcttaaa gcagattgat gccaatatta aggttgacga caaccgtttt gagcaggagg
    16501 ttatttacta ccttgaaaag cttgacatta ccgaggaaaa agtccgtttg cgacagcatt
    16561 gcagctactt tacggaaaca gttaaccagc ctgaatcggt tggacgtaag cttgggttta
    16621 tagcccagga aatgggacgc gaaataaaca ctctgggttc aaaggcaaac aatgccgaaa
    16681 tccaaaaaat tgtggtgatg atgaaggatg agcttgagaa aatcaaggag caatcgttaa
    16741 acatcttgta ggtaatggaa ggcaagctat taatattttc agccccttcg ggtgcaggta
    16801 aaactaccat tgtaaggcat ttgttggaaa aatttcctca acttgaattt tcggtatcgg
    16861 cttgcagccg aatgccgcgc gacggagagg ttaacggtaa ggattactat ttccttactg
    16921 ttgatgaatt ccggaatagg attgagaaag gcgaatttgt ggagtgggaa gaggtttatc
    16981 cagggtcata ctacggaacg ctgtggtctg aagttaaacg tatttgggac aaaggacacc
    17041 atgtaatgtt cgatgtagat gttaaaggag gtgttaacct caagcggaag tttccgaata
    17101 actcgttggc agtatttgtg atgcctcctt ccattgacga gctgcgtgaa cgactactta
    17161 aaaggggaac agaaaccgag gagtcgctaa aggtgcgtat tggtaaagca caggaggaaa
    17221 tgacctatgc aaatcagttc gattatgtat taaccaataa tcaggt
//
LOCUS       DAOSAL010000054        17118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_2308_length_17118_cov_47.353689, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000054 DAOSAL010000000
VERSION     DAOSAL010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 17118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17118
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2308_length_17118_cov_47.353689"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="PK049_08145"
     CDS             <1..455
                     /locus_tag="PK049_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61568.1"
                     /translation="ARKFGEDEDVWGIAGLLHDLDVEITNADPKSHALVAAEMLQNVL
                     PAEAVDAIKMHNEEATGMPRTTRFQHALAAGETITGLIFATALVYPDKKIASVKPKSV
                     VKRMKEKQFAASVNRDTIMECERIGLSLDEFVQLSLNALIPIAPTLGF"
     gene            466..>1064
                     /locus_tag="PK049_08150"
     CDS             466..>1064
                     /locus_tag="PK049_08150"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOK61569.1"
                     /translation="MNIDLVLPLVIDAAQRAGEWALSEREKFTAEMVEVKGKHNFVSY
                     VDKHCEAMLVETLGKALPQAGFIAEEGTGSKNSSGLNWIIDPLDGTTNYIHGLSPFAV
                     SIALMDNDRVVLGVVYEASLRECFWATESGGAFLNGNPIKVTTTQKVNDSLIATGFPY
                     YDYSRISPFFQTMDYFMRNSHGLRRLGSAATDLVYVACGR"
     assembly_gap    1065..1164
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1165..1960
                     /locus_tag="PK049_08155"
     CDS             <1165..1960
                     /locus_tag="PK049_08155"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOK61570.1"
                     /translation="IYKIIGYRVDVVKKNLKNSFPQKTESELLEIERKFYRHFCDLFV
                     EQFYILHANAKKAKRLCSFKNLEQLDRYYNENKSIVVAGGHYGNWELYGLFGLFLKHQ
                     VFGAYKPLANKYFESFVNASRERFGALAVPMRDTPRTAMQFAREGKLFFLGLIADQTP
                     AKGEIRYWTTFLNQDTPVFLGTEKIAKKLNQPVFFCNMRKLKRGFYEVELELLTDKPA
                     ETKPYEITEMHVRALERLINEAPEYWLWSHRRWKHSRNLTQTTNEQ"
     gene            1950..2978
                     /locus_tag="PK049_08160"
     CDS             1950..2978
                     /locus_tag="PK049_08160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOK61571.1"
                     /translation="MSSSKVAVVILNWNGEHFLKKFLPTLIENTTRSQVEIVVADNGS
                     TDGSLLILNDYPTVRTILLDKNYGYTGGYNRALSQVEAQYYVLLNSDIEVPSGWLEPM
                     VTFMDKNPQVAACAPKLIDFNRKEYFEYAGAAGGFIDLFGYPFCRGRILSEIEADNGQ
                     YDDTMPIFWASGACMMVRSSCFWEVGGFDDDFFAHMEEIDLCWRLQNRGYIIYSIPSS
                     VVYHVGGGTLPNNNPFKLFLNYRNNLSMLHKNLSLRALLVVIPVRMVLDILSAFVYLV
                     NGRLSFTNAVFKAHKQYLANISTLNKKRRLLKPFNQATRYRGLILVDFFILRRRKFYS
                     LPISGNNY"
     gene            3187..4821
                     /locus_tag="PK049_08165"
                     /pseudo
     CDS             3187..4821
                     /locus_tag="PK049_08165"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
     gene            complement(4906..6045)
                     /gene="lysA"
                     /locus_tag="PK049_08170"
     CDS             complement(4906..6045)
                     /gene="lysA"
                     /locus_tag="PK049_08170"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOK61572.1"
                     /translation="MQTPFYQYDLALLNETLNAAAAQAARYGYTIHYALKANANPALL
                     KIISGAGFGADCVSGNEIKVALKNGFKPQGIVFAGVGKTDWEIEYAIEQNIQCIHCES
                     VQEFQVIEQIAQKLNKTASIALRINPNVDANTHKSITTGLKENKFGMSAEEASLILNS
                     ANKYKNINLVGLHFHIGSQIMNMEVFKNLALEVNRIQERLQSKALPYLNLGGGLGIDY
                     INPRINRIADFAGYFKTINQWLNPGKEQTIHFELGRSLVGQCGQLYTRVLYTKTTKSR
                     NFAIVDAGMNALIRPALYGAIHRIENISKIEYPTIATYDVVGPICETTDCFGEQVNLP
                     NTERGDLLVIHSCGAYAESMASDYNLRERPKSVFNHSGLALLPKH"
     gene            complement(6048..7409)
                     /locus_tag="PK049_08175"
     CDS             complement(6048..7409)
                     /locus_tag="PK049_08175"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOK61573.1"
                     /translation="MKVLKFGGTSVGNADRLRKVSELLNGDEPIVVVLSAMAGVTNSL
                     VEITELAQSGKMLEAIEKTKELEDKHIKTADRLFDTKMYRKRGEEFISNLFLRILSRL
                     IEGFNQTDSRNIVALGEQLSVGLFHMLLTEQGKRAAYIPALEYMRIDKDNEPDFFYIE
                     QNLKRLMQNNPFAKIYVTEGFICRNANGLVDNLGRGGSDLTAAIVGNVLNANEVQIWT
                     DIDGVHNNDPRFVTNTFPLRQLSYDEAAELAYFGAKILHPQTIQPCKVKNIPVVLKNT
                     LNPTDEGTVISSMKNRKGIKAVAAKDGITAINIKSSRMLLAYGFLHRVFEVFDTYKTP
                     VDMITTSEVSISLTIDSTINLEKIVNALSQFAQVEVERNQTIVCVVGDFPASHKGYAA
                     MVFDCLREIPIRMISYGGSVNNISFLIDSHYKVQALRLLQQLFPAVSVSDSTHQNIEA
                     NTF"
     gene            7800..10253
                     /gene="pheT"
                     /locus_tag="PK049_08180"
     CDS             7800..10253
                     /gene="pheT"
                     /locus_tag="PK049_08180"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOK61574.1"
                     /translation="MKISHSWLTQYLNVSKTPDEISKILTSIGLEVESLEAKETIKGG
                     LAGVVVGEVLTCEKHPDADKLSLTTVNVGVDQPLSIVCGAPNVAKGQKVLVATVGAKL
                     YFNDQEIVIKKAKLRGQLSEGMICAEDELGLGDSHDGIMVLPADAPVGMPAADYLRIE
                     KDYQYEIGLTPNRIDAASHYGVARDLAAYLTYHESPVKANLPDVSNFKVDNNTLPIPV
                     EVLNTEACPRYCGITINGVKVAPSPEWLQKRLRSIGLNPINNVVDVTNFVLHEVGQPL
                     HAFDADAIKGNKVIVRTMPEGTPFVTLDGVERKLSADDLMICNESEPMCIAGVFGGLH
                     SGVTEKTTKVFLESACFNPVYVRKTGRRHDLHTDASFRFERGTDPNITVWALKRAALL
                     IKEVAGGTISSEIVDVNSGPVNHFEVDFNIHRAWSLIGKQIPEVDIERILEGLEIRVL
                     KKNGQQWLLGVPTYRVDVRREADVVEDILRIYGYNNVEIGAKVNGTLSYTKKPDSLQM
                     QNIVSDYLSSNGFAEIMCNSLTKGGYYEGLATFPEVNSVKILNPLSNELNVLRQTLLF
                     GGLESIQRNINFRNLNLKLYEFGNCYVFKPEVKSQKPLDKYSEHFSIGIWLTGDNIKE
                     HWMTKPERQNFYHLKGYVHSILSRFGFSIDRIEASEAPGDIYAYGLTYKAQGKHLVTY
                     GMVSPEILRQFDIKTEVFFAEIMWLPLMDMIAESKIQYQELPKFPEVRRDLALLIDSS
                     VKYEQIRKLAFETERKLLKSVNLFDVYQGKNIPEGKKSYAISYILQDESKTLTDEQID
                     RTMKRLIDVYVRELGAQIR"
     gene            10263..10769
                     /locus_tag="PK049_08185"
     CDS             10263..10769
                     /locus_tag="PK049_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HOK61575.1"
                     /translation="MAKIELVLGDITQMDVDAIVNAANESLLGGGGVDGAIHRAAGPQ
                     LLEECRTIGGCPTGEARITKGYNLKARYVIHTVGPIWRGGQHNETLLLRHAYVNSLKL
                     APKHGLKSIAFPNISTGVYHFPKQSAAQIAIRAVMEFTAKNHLPERIVFVCFDQENYS
                     IYSELLGK"
     gene            complement(10772..12154)
                     /locus_tag="PK049_08190"
     CDS             complement(10772..12154)
                     /locus_tag="PK049_08190"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOK61576.1"
                     /translation="MMKEISILVLDDEKVFRKEIKEFLDGEGFSVLTAERPSEAFSIL
                     SENQIDILILDLRLPEMDGLQVLTKVKEEYPDIEVIMITGHGDMDAVINAMRLGAIEF
                     FPKPFRLLDMRAAIQRTKRYIALHEQLKEINQTYSLVSKDLIESIGTEIIGNSFGIRQ
                     VVDLMGKVAKTDNTSVLITGESGTGKELVARGIHYLSSRKKSYFYAVNCSAIPDTLFE
                     SEFFGHKKGAFTGANEDKAGWFEVANGGTLFLDEVVEMQPAMQSKLLRVLEERKIRRI
                     GSSVDIPVDVRIIAATNQDINKLIEEGKFRSDLYYRLNSFQIHIPPLRERKVDIPILF
                     DYYVKHISTRMGKKITQVDPTIVKAMMGYNFPGNIREMRNMVERAIILSEGGRLTMKN
                     FSGLIAPTDSVSTTGIIGEDIFDLDLIEKIVIMKALERTGYKKTEAAQLLNITRQALD
                     RRLEKYDINL"
     gene            complement(12176..14410)
                     /locus_tag="PK049_08195"
     CDS             complement(12176..14410)
                     /locus_tag="PK049_08195"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOK61577.1"
                     /translation="MKHLLRIVWLTLSLAGLAQIGLSQTDTLPQEIAVKGSSKAADLV
                     VQGKSLLKTNPDRCLDIGLQALVTAKKEGDSFTESMALELLAEANYKLGEYETSLRYF
                     NRAANLYSSINKPEFAAKSLLGMARVYEASQNLDLAIQQLEAGIKTLKNIPNKNVYLE
                     LILKLGNLHLSQKNFKQAKQYGIEALTLLNNQATSVYNKNKALIDTYTLLGQASKNSG
                     DLGTSLDYFKKVSEAAIKQSDSTSYSKSLNEIGVVFMLTQKYDSAYQYFNLSYTISLN
                     ASDTVGIIQSLQGLGDYYYEKGNYNQAINYYTQCQSISSKYNDIPTLIAALVSISRCH
                     FQLGDYPTSSRFLNQALNIAQKNNLTSSKADVYRYLSVINEAQGRYKDALDYHKMWVD
                     LRDSLQYEETGQRLAKLQILYEISQKEKENEILKQNSEIQKLQIAKSRYQMRFLISML
                     VVVAIILSLLVVLFRNKQKEIAKQKETEQRITEINRELERRMIEEIKKQEKQQQLLSQ
                     KSKLESLGTLAAGIAHEINQPLGGISMGLDNILMRIQDKNCSEEYLKEKLNSLFENVE
                     RIKKIIDHIRYFSRTQKPVSFSTVNLNDVVKSALFMVSAQFENHGVIIETSLDENIPP
                     ITADKYKLEQVLLNLLSNAKYAVDEKEKKSEDKTYRKKIKIGTRKDEQNLYLTVWDNG
                     TGIPQKIVEKIFDPFFTTKSEDKGTGLGLSISYGFIKDLLGDIRVESEPNEYTLFEIS
                     IPRE"
     gene            14486..15349
                     /locus_tag="PK049_08200"
     CDS             14486..15349
                     /locus_tag="PK049_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HOK61578.1"
                     /translation="MFFKVKNYLSLVKFSHTIFAMPFALIGYFLAYYKHSLNPDWWLF
                     LKVILCMVFARNAAMSFNRLVDRHFDKLNPRTSGREIPRGIIAPRSVMLFCIVNSILF
                     ILTTFFINPLVFYLSPIALGVILFYSYTKRFTMFSHLVLGIGLSLAPIGAYLSVTGSF
                     ALIPILYSVVVLFWVAGFDIIYALQDEEFDRNSKLHSIPAAVGARGALVIALVLHFMS
                     ALIVIAIGILAGFNIYYWIGAFLFIALMIYQHYLVRPTDISKVNMAFATTNGMASLLF
                     ALFNIISILTC"
     gene            15519..15944
                     /locus_tag="PK049_08205"
     CDS             15519..15944
                     /locus_tag="PK049_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61579.1"
                     /translation="MKKYIVFFGLLSLILGCSGNQNSANKVELVDGQPVIDVTVDSLL
                     LNAKNYESKVVRVSGIVEHVCVHTGLRITLQGKSPEMKLSVKTSDASRPFDMSMKGAK
                     ATVTGVFKRVGGANEVCDVESAHHPGEIYFLECNSINVK"
     gene            15947..16498
                     /locus_tag="PK049_08210"
     CDS             15947..16498
                     /locus_tag="PK049_08210"
                     /inference="COORDINATES: protein motif:HMM:NF015863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HOK61580.1"
                     /translation="MTLSKFLRVIHRDCSYFFAGMVLLYSVTGILLNHGHQLNPYYYI
                     DVKTVKVDIPRDSSKVNDQFLLGVFSPFGESEIRSHYYPDSSTLKVFFKNGTATVNLN
                     EQTALIENSIRRPILYQLNMMHRNRPKAWWTWFSDIFAVGLIAITLTGLFLIKGKNGI
                     TRRGAVLVAAGLILPILAMIIFF"
BASE COUNT         4525 a         3552 c         3845 g         5096 t
ORIGIN      
        1 tggcccggaa gtttggtgag gatgaggatg tttggggtat agccggtttg cttcacgact
       61 tggatgttga aataaccaac gccgatccta aaagccatgc gctggttgct gctgaaatgc
      121 ttcaaaatgt attgccagcc gaggctgttg atgccattaa aatgcacaac gaggaggcaa
      181 caggcatgcc tcgtacaact cgtttccagc atgccctggc cgccggcgaa accataaccg
      241 gcctaatatt tgccacggcc ttagtttatc ccgataaaaa aattgcaagc gttaagccaa
      301 agtcagtggt aaaacgtatg aaggaaaaac agtttgcagc ctctgtaaac cgcgatacaa
      361 taatggagtg cgaaaggatt gggcttagcc tggatgagtt tgttcagctc tccctaaacg
      421 ctttaatacc aattgcgcca actttaggtt tttaatggtt cgataatgaa tattgattta
      481 gtacttcctc tggtaattga tgctgcccaa agggcaggtg agtgggcttt gtccgaaagg
      541 gaaaagttta cagccgaaat ggttgaggtg aagggaaagc acaactttgt ttcgtatgtc
      601 gataagcatt gcgaggccat gctggttgaa acattgggca aagcgcttcc gcaagccggt
      661 tttattgccg aggagggaac cggtagcaaa aattccagcg gtttaaactg gataatcgac
      721 ccgctcgatg gcaccacaaa ctacatccat ggactatcgc cttttgctgt aagcattgca
      781 ttgatggaca atgatagggt tgttcttggt gtagtgtatg aggcaagcct aagggagtgc
      841 ttttgggcaa ccgaaagtgg tggtgctttc ctgaacggaa accccattaa ggtaaccact
      901 acacaaaagg taaacgattc gcttattgca actggtttcc cgtactacga ttactcccgc
      961 atcagcccct ttttccagac catggattac ttcatgcgca actcgcacgg cttacgccgt
     1021 ctgggttcag ctgctaccga cctggtttac gttgcttgtg gccgnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnncattta taaaattata gggtataggg ttgatgttgt
     1201 gaaaaaaaac ctgaaaaatt ctttccctca aaaaacagaa agtgagttgc tggaaattga
     1261 aaggaagttt taccgccact tctgcgattt gtttgtggag cagttttaca ttctgcatgc
     1321 caatgctaaa aaagccaaac ggttatgtag ttttaaaaat ttagagcaac ttgaccggta
     1381 ttacaatgaa aacaaaagca ttgttgtggc tggcggacat tatgggaatt gggaattgta
     1441 tggtttgttt gggctttttt taaagcatca agtttttggt gcatataaac cattagctaa
     1501 taagtacttt gagagttttg taaatgcttc gcgagagcga tttggtgcgc tggctgttcc
     1561 catgagagac actcccagaa ccgcaatgca atttgccagg gaagggaaac tattctttct
     1621 gggattaatc gccgatcaaa ccccagctaa gggcgaaata aggtactgga caaccttttt
     1681 aaaccaggat acacctgttt tcctgggtac cgagaagatt gccaagaaac tcaaccagcc
     1741 ggttttcttc tgcaacatgc ggaagcttaa aagaggtttc tatgaggttg aacttgaact
     1801 cttaaccgat aaacctgctg aaaccaagcc ctacgaaata accgaaatgc acgtaagagc
     1861 attagagcga ttgattaacg aggctcctga gtactggctt tggtcgcacc gacgctggaa
     1921 acactcccgt aatttaaccc aaactaccaa tgagcagtag taaagttgcg gtggttatcc
     1981 tgaactggaa tggggagcat ttcctgaaaa agttccttcc tacgcttatt gagaacacta
     2041 caaggtctca ggttgaaata gtggttgccg acaatggttc aaccgatggc tcgcttttga
     2101 tacttaatga ttacccaacg gttcggacaa ttctcctcga taaaaactac ggatatacgg
     2161 gtggatacaa ccgtgccctt agccaggtag aggctcagta ctacgtgctc ctgaactccg
     2221 atatcgaagt gcccagtgga tggttggagc ccatggttac atttatggat aaaaaccccc
     2281 aagtggccgc gtgcgctcct aagctaattg attttaaccg caaggaatac tttgagtatg
     2341 ctggggcagc aggaggtttt atcgatctgt ttggttatcc attctgccgg ggacgaatcc
     2401 tttccgagat tgaagccgac aatggccagt acgatgatac catgccaata ttctgggcaa
     2461 gcggagcttg catgatggtt cgctcatcat gtttctggga ggttggaggt ttcgatgacg
     2521 atttttttgc ccacatggag gaaattgatt tgtgctggcg cttgcagaat aggggctaca
     2581 taatttactc cattcccagc tcagtggttt accatgttgg agggggaacg ctgcctaaca
     2641 ataacccctt caagcttttc ctgaactaca ggaataacct ttccatgttg cataaaaacc
     2701 tttcacttag ggcgctcctt gtggttatcc ctgtaagaat ggtgcttgat attctttcgg
     2761 cttttgttta ccttgttaat ggtcgccttt cgtttactaa tgctgtgttt aaggctcaca
     2821 aacagtattt agccaacatc tctactttaa ataagaaacg gagactcctt aaacctttca
     2881 atcaggctac ccgctacagg ggattaattc tagttgattt tttcatactc cgccgtcgta
     2941 aattttatag cttgccaatt tctggtaaca attattaacc attcaaaaac ccacatttca
     3001 aacgattgtt ccaatcgctt tgtcgtttat cacatgtaaa aaacgaacat ttagttagat
     3061 attgtcgtta ataggtatat atttgcatct tggatatcga tgcattgcct ttagagtttc
     3121 acgtaacaca catttatggt ttcatttaag aagttaacaa tcagggatat actgttccgt
     3181 tcgcgaatgg catattacaa caggccatca ttagcatttg ttggtcagga gcctgtaacc
     3241 tatgccgata tggcagtaat agtccgaaat gtttcgggca tgctaaacac ccttggtata
     3301 ggcaaaggcg ataaggtggc actaattggc gagaatagcc ccaactgggg aatggcctac
     3361 cttgccatta catcaatggg tgctgtagtg gtgccaattc ttcctgactt ttcggggcac
     3421 gagatggaaa gtattattaa ccatagcgaa gctaaggttg tttttgtctc agccaagcta
     3481 tatggtcaac tcgatagaaa gaacattccg ggtgtaattg cttacatact aatcaataac
     3541 ctacagccct ttgacgagag cgttaccacg cctacggctg gtatgcctta cgaaacgcca
     3601 aaggctattg tgaatgttga ttactgcgat tgcgaactac ctcgccccga tgaggatgac
     3661 cttgcagcaa ttatttacac atcgggtact actggtcgcc ccaagggtgt aatgctcacc
     3721 cataaaaacc tggtgtcaaa cgtactatca accttgatga tacaggaggt aaacgaaaac
     3781 gacaggttgt tatccattct tccgctttcg catacctatg agtgtactat aggcttctta
     3841 atacctatgg caaccggggc atccgtttac tatctggata agccacctac accagctgta
     3901 cttattcccg ccatgcaggc tgttaagcct accatgttgc tttcagtacc cctagtaatt
     3961 gaaaagattt acaagaataa gattaagcct gaacttacat caagctctct caagcgaaaa
     4021 ctttacaact ttacccctac ccgcaagctg cttcaccgga ttgcagcaaa ataactccat
     4081 aaaacatttg gtggggagct tcactttttt ggcattggtg gtgcaaagct gtcgtttgag
     4141 gctgaacgct ttctgaacga gggtcgtttc ccgtattcca ttggttacgg tatgaccgaa
     4201 acatcgccac ttttaacggg aagttcacca aagctggtta ggttccgtac agcaggtttt
     4261 tccattcccg gacaggaact gaagttgcta aaccctaacc cacatacagg ggagggtgag
     4321 ataattgtaa ggggtgccaa tgtaatggtt ggctactaca aggatcctga acttacccaa
     4381 accatgttta ttgacggttg gctccgtact ggcgatttag ggtatattga cagcgatgga
     4441 tacgttgaaa ttcgtggacg ctcaaagaat atgatcctta ccgcttccgg cgaaaatatt
     4501 tatcccgaag agattgaaga cgtaatcaac aagcatgagt ttgtgcttga atcgctggtt
     4561 tacgagcttc gtggaaaact tgtggctcgt gtccacctga acaaggaagc tcttgagaaa
     4621 tactacgaga atcttaaaca ttctgccaag gattggcaac acaatgctga ggatcatacc
     4681 cgaaaggtgt tagacgatat aatgacctat gtaaatgcta aggtttcaaa gttctcccga
     4741 ttgaatcata ttgttgagca acaggaaccc tttgtaaaga cacctactca aaaaattaaa
     4801 cgattccttt acaaggagta aaattcatta atgaagtaaa agtcaaaggc tgccattggc
     4861 agcctttggt gttcaaaatg agttagtgta cgttacctaa tcttactagt gtttaggcaa
     4921 taaggctaaa cctgaatggt taaaaacaga ttttggccgt tccctcaggt tgtaatccga
     4981 ggccatggat tccgcatagg cgccacagga atggataaca agcaaatcgc cacgttctgt
     5041 attggggagg tttacctgct cgccaaaaca atcggttgtt tcgcaaattg gcccaaccac
     5101 atcgtaggtg gcaatagtag ggtattcaat tttacttatg ttctcaatgc ggtgtatggc
     5161 gccatagagt gccggacgga ttagggcatt catgccagca tctacaatgg caaagtttcg
     5221 cgatttagta gttttggtat acagcactcg ggtgtatagc tgtccacatt gaccaaccag
     5281 tgagcggcca agctcaaaat ggatagtttg ctcctttccg gggttgagcc actggttgat
     5341 ggttttgaaa taccctgcaa agtcggctat cctattaatc ctgggattga tatagtcaat
     5401 tcccaaacct ccacccaggt ttaggtaagg gagtgctttg ctttgtaacc tttcctgaat
     5461 acggttaacc tcaagggcta ggttcttgaa cacttccatg ttcattatct gtgaaccaat
     5521 atgaaagtgt agtccaacca ggttgatatt cttatactta tttgcagagt ttaatatcaa
     5581 acttgcctct tccgcactca ttccaaactt attctctttt agtccggtgg taattgactt
     5641 gtgggtgttg gcgtccacat tagggttgat gcgaagcgct atactggcag ttttgttcaa
     5701 cttttgtgca atctgttcaa ttacctgaaa ttcctgaacc gactcgcaat ggatgcattg
     5761 aatattctgc tcaatggcat actcaatttc ccaatcggtt ttaccaactc cggcaaacac
     5821 aatgccttgg ggcttaaatc cgtttttcag ggcaacttta atctcgtttc cgctaacaca
     5881 atcggcgcca aaacctgcac ccgaaatgat cttcaaaagg gcagggttgg cattagcttt
     5941 aagtgcgtaa tgaatagtgt acccatacct tgctgcttgc gcagctgctg cgttcagtgt
     6001 ttcgttcagt agcgctaaat cgtactggta gaatggagtt tgcatggcta aaaggtgttt
     6061 gcttcaatgt tctggtgggt tgaatcactt acactcaccg ccgggaaaag ctgctgtagc
     6121 aaccttaagg cctgaacctt atagtgcgaa tcaatcagaa acgagatatt gtttacgctt
     6181 cctccgtagg agatcatccg tataggtatc tcgcgcaggc aatcgaaaac catggctgca
     6241 tagcccttat gcgatgctgg gaaatcgcca actacgcaaa caatggtttg gttccgttcc
     6301 acttccacct gtgcaaattg gcttaaagca tttacaatct tttcaaggtt aatagtgcta
     6361 tcaatggtaa gcgatatgga tacctccgat gtagtaatca tatcgacagg ggttttgtat
     6421 gtgtcaaaaa cctcaaaaac cctgtgcagg aagccatagg ctaaaagcat ccgcgacgac
     6481 ttgatgttta ttgctgtaat accatccttt gctgccactg cctttatccc tttccggttc
     6541 ttcatggatg aaattaccgt tccttcgtct gtagggttca aagtgttctt caacacaaca
     6601 gggatattct ttaccttgca gggttgaatg gtttgggggt gaagtatttt tgccccaaag
     6661 taggcaagtt cggcagcctc gtcgtacgat agctgtcgca atgggaaagt atttgtaaca
     6721 aagcgtgggt cgttgttgtg aaccccatct atatcggtcc aaatttgcac ctcgtttgcg
     6781 ttcagcacat ttccaacaat ggctgcggta agatcgcttc cacctcggcc caggttatca
     6841 accaacccat ttgcattgcg gcaaataaaa ccctcggtta cataaatttt tgcaaaaggg
     6901 ttattttgca tgaggcgttt taggttctgt tcaatgtaaa agaaatcggg ctcgttatcc
     6961 ttgtcaatcc tcatgtattc cagtgctgga atgtacgcgg ctctttttcc ctgctctgtt
     7021 agtagcatgt ggaatagccc taccgatagt tgctctccta gggctactat attccgcgaa
     7081 tcggtctggt tgaagccttc aatcaatctc gatagaattc gcagaaacag gttgctaata
     7141 aattcttcac ccctttttcg gtacattttt gtatcaaata gtctgtcggc ggtttttatg
     7201 tgcttgtcct caagctcttt agttttttca attgcctcga gcatcttacc cgactgtgcc
     7261 agctctgtga tttccactaa gctgtttgta actcctgcca tggccgataa aaccactaca
     7321 atgggttcat cgccattaag cagctcgctt acctttctga gcctatcggc attaccaacc
     7381 gatgttccgc caaactttaa aactttcatt ttcttttttt ctgatttttt aggggtttaa
     7441 cataaaaaaa ggcctgcagg gttctgcagg cctaacactc aatatcattg taatactttt
     7501 atgctgattt tatcaatgtg tcaacggcct gcttgtgggt ctgttttttc atggtaataa
     7561 ccatctgttt catgcaaatg gtttgcatga aaaactgttt attactcatg gtatagcaag
     7621 ccgaaaattc catttttgtg ctatttcact tttgcaaaaa tacttttttt ctaatatcaa
     7681 accagctttt aggaaaaaat gtttttattt tttatgaagc acttttgtgg gtacgattga
     7741 taaatgttac ttttgttggc taaaattgat tgacaacaag ttgactaaat cttttctgta
     7801 tgaaaatttc gcatagctgg cttacgcaat acctgaatgt gagtaaaaca cccgatgaga
     7861 tttcaaagat actaacatca attggattag aggttgaaag ccttgaggca aaggaaacca
     7921 ttaaaggtgg ccttgccggg gttgtggtgg gcgaggtttt aacatgcgaa aagcatcccg
     7981 atgccgataa gctgagctta accacggtaa acgttggtgt cgaccaaccc ttaagcattg
     8041 tatgcggtgc acccaatgtg gccaaaggcc aaaaggtact ggttgcaacc gttggagcca
     8101 agctgtattt caacgatcag gaaatagtaa ttaaaaaggc aaaacttcgc ggtcagctct
     8161 ccgagggtat gatatgtgcc gaggatgagt tgggtttagg cgatagccac gatggtataa
     8221 tggttttacc tgccgatgct cctgtgggca tgcctgctgc tgattaccta agaattgaga
     8281 aagattacca gtacgagata ggcttaaccc ctaaccgaat tgatgctgcc tcgcattatg
     8341 gtgtggctag ggatttggct gcctacctaa cctaccatga gagcccggtt aaggccaatc
     8401 ttcctgatgt ttccaatttt aaggttgata acaacacatt gcccataccc gttgaggttt
     8461 tgaacactga ggcatgcccg cgttactgcg gtatcaccat taacggggta aaggttgccc
     8521 cttcgcctga gtggttgcag aaacgattac gttcaattgg gctcaatccc attaataatg
     8581 tggttgatgt taccaatttc gttctgcatg aggttggtca accactccat gcttttgatg
     8641 ccgatgccat taagggcaac aaggtgattg ttagaaccat gcctgagggt accccttttg
     8701 ttaccctcga cggagtggaa cgcaagctat cagccgatga tttgatgatt tgcaacgaga
     8761 gtgagcccat gtgtattgcc ggtgtttttg gtgggttgca ctcgggagtt actgaaaaga
     8821 ctacaaaagt attccttgaa agcgcttgct ttaaccctgt ttacgtccgt aaaaccggcc
     8881 gtaggcacga tttgcatacc gatgcctcgt tcaggtttga gcgcggtact gaccccaata
     8941 ttacagtgtg ggcactaaag cgtgcagcgc ttttgattaa agaggttgct ggcggtacta
     9001 tatcgtcaga gattgtggat gtaaatagcg gtccggttaa ccacttcgag gtggatttca
     9061 acatccacag ggcatggagt ttaattggaa agcaaattcc cgaagttgat atcgaaagaa
     9121 ttcttgaggg tcttgaaatt agggtgctta agaaaaatgg acagcagtgg cttttaggag
     9181 ttcctacata cagggtagat gttcgtcgtg aagccgatgt ggttgaggat attctcagga
     9241 tttacggcta taataacgtt gaaataggcg ctaaggttaa tggaacatta agttacacca
     9301 aaaagcccga tagccttcag atgcagaaca ttgtatcgga ctacctttcg tcaaatggtt
     9361 ttgctgaaat tatgtgcaac tcgttgacaa agggcggtta ctatgaaggg ctggctactt
     9421 tccccgaggt aaactcagtt aagattctta accctctaag caacgagctg aatgttttac
     9481 gccaaacact cctttttgga gggctcgaga gcattcagcg aaacattaat ttccgtaacc
     9541 taaaccttaa gctctacgag tttgggaact gctatgtatt caagcccgaa gtaaaatcgc
     9601 agaaaccgct tgataagtac tcggagcatt ttagcattgg catttggtta accggcgata
     9661 atattaagga acactggatg accaagcccg aaaggcagaa tttttatcac ctgaagggtt
     9721 atgttcattc catactatcg cgctttggtt ttagtattga tagaattgaa gcatcagagg
     9781 ctcctggtga tatttacgct tatggattaa cctacaaggc acagggtaag catcttgtta
     9841 catatggaat ggtaagccca gaaatactca ggcaattcga tataaagacc gaagtttttt
     9901 ttgccgaaat aatgtggctg cctttaatgg atatgattgc agaaagcaaa attcagtacc
     9961 aggaattacc caagttccct gaggttcgtc gcgatttagc tttgcttatc gattcatcgg
    10021 taaagtatga gcaaatccga aagctagcct ttgaaaccga gcgcaagctc ctaaaaagcg
    10081 taaacctttt cgatgtttat caagggaaga atataccaga ggggaagaag tcgtatgcaa
    10141 tcagttatat ccttcaggac gaaagcaaaa cccttaccga cgagcaaata gataggacaa
    10201 tgaagcgcct aattgatgtt tacgttaggg agcttggtgc gcaaatccgt taaaagtaaa
    10261 ctatggccaa aattgaattg gtgttaggtg atataaccca aatggatgtt gatgccattg
    10321 taaatgcagc aaacgagtcg ttgttgggtg ggggtggtgt agatggggct atacatcgtg
    10381 ctgctggccc gcagctgctc gaggagtgta gaaccatagg ggggtgcccc acgggtgaag
    10441 cgcgaatcac caaaggatat aacctaaagg cacggtatgt aatccatacc gttgggccaa
    10501 tttggagggg tggccagcac aacgaaacgt tactactccg gcatgcttac gttaacagcc
    10561 taaagttagc gccaaagcat gggttaaaaa gcattgcttt cccaaatatt agtaccgggg
    10621 tttaccattt cccaaagcaa tcggctgcac aaattgccat aagggcagtt atggaattca
    10681 ctgctaaaaa ccatttgcct gagcggattg tatttgtttg ttttgatcag gagaactatt
    10741 ctatttacag tgaacttcta ggcaagtaag tttataggtt tatatcgtat ttttccagcc
    10801 ttcggtctag tgcctgacgg gtaatattta acaactgtgc tgcctcggtt ttcttgtaac
    10861 ccgtacgctc aagggctttc attatcacta tcttttcaat tagatcgaga tcaaagatat
    10921 cctctccaat tattcctgtt gtgctgactg aatcggttgg agctattaga ccgctaaagt
    10981 ttttcatggt taatctgccg ccctcggaga gaattatggc acgttcaacc atattacgca
    11041 tctccctgat gttgcccggg aagttgtaac ccatcatggc cttaactatg gttgggtcaa
    11101 cctgggttat ctttttgccc attcgggttg aaatgtgttt aacgtagtaa tcgaaaagga
    11161 ttggtatgtc tacctttcgc tcgcggagcg gaggtatgtg tatttggaat gagttcaggc
    11221 ggtagtaaag atcgctgcgg aacttaccct cttcaataag tttgttaatg tcctggttgg
    11281 tggctgcaat aattcgcaca tcaacgggga tatccacact tgagccaatt ctacgaattt
    11341 ttcgttcttc caggacgcgt agtaacttac tctgcatggc aggttgcatt tcaaccacct
    11401 cgtcgaggaa gagcgtacca ccatttgcca cctcaaacca gccagcttta tcctcgttgg
    11461 ctccggtaaa tgcccctttc ttgtgaccaa aaaattcgct ctcgaacagg gtgtcgggaa
    11521 tggctgagca gttcactgcg tagaaatacg attttttacg tgagctaagg tagtgaatgc
    11581 ctcgggcaac aagttccttt ccggttccgc tctcgccggt tataagtact gaggtgttgt
    11641 cggttttagc caccttgccc atgaggtcaa ctacctgtct aattccaaag gagttaccaa
    11701 taatctcggt accaatgctt tcaatcaaat cctttgatac caaagagtat gtttggttaa
    11761 tctcttttag ctgctcgtgt agggcgatat agcgtttggt gcgttgaatg gcagctcgca
    11821 tgtcgagcaa gcggaagggt tttgggaaga actcaatagc tcctaagcgc atagcattaa
    11881 taactgcatc catgtcgccg tgcccagtaa tcatgataac ctcaatgtcg gggtattcct
    11941 cctttacctt ggtgagcacc tgtaggccat ccatctcggg gaggcggagg tcaaggataa
    12001 gtatatctat ttggttctcg ctgaggatgc tgaatgcttc cgacggtcgc tctgctgtaa
    12061 gaacggaaaa gccttcccca tccagaaact cttttatctc tttacgaaaa accttttcgt
    12121 cgtcgagaac caaaatgctt atctctttca tcattctata atgatttgtg gtttattact
    12181 ctctaggaat tgatatttca aaaagggtgt attcattagg ttcgctttca acgcgtatat
    12241 cgcctaaaag gtctttgata aacccatacg atatggataa ccctaaacca gtccctttgt
    12301 cctcgctttt ggtggtgaaa aatgggtcga aaattttctc tacaattttt tggggaatgc
    12361 ccgttccgtt gtcccaaact gttaggtata ggttttgctc atctttacgt gtgccaattt
    12421 taatcttttt tcgataggtc ttatcctcac ttttcttttc tttttcatca actgcatact
    12481 ttgcattgga aaggaggtta agcaaaacct gctctaactt atacttgtca gccgtaattg
    12541 gtggaatgtt ttcatccaat gaggtttcaa tgataacccc atggttttca aattgagccg
    12601 ataccataaa gagggcactt tttaccacat cgtttaggtt gaccgttgaa aaagaaacag
    12661 gcttctgagt acgggaaaaa tagcggatgt ggtcaattat ctttttaata cgctcaacat
    12721 tctcaaacag cgagtttagc ttctccttta ggtactcctc gctacagttt ttgtcctgaa
    12781 tacgcataag gatattgtcc agccccatcg atataccacc cagtggctga ttgatttcat
    12841 gggcaatacc ggctgcaagg gtaccaagcg attcaagttt tgacttttgc gagagcaatt
    12901 gctgttgctt ttcctgtttc tttatctctt caatcattcg ccgttcaagc tctcggttta
    12961 tctcggtgat acgctgttcc gtttcctttt gcttagcaat ctctttctgt ttattgcgga
    13021 atagcacaac cagtagcgag agtattattg ccacaaccac aagcatcgat atcagaaaac
    13081 gcatttggta cctcgacttc gctatctgta acttttggat ctcggagttt tgctttagga
    13141 tttcgttctc tttttccttt tgcgatatct cgtaaagtat ttggagtttc gccagtcgtt
    13201 gtcctgtttc ttcgtactgc aatgaatcgc gcaggtcaac ccacatcttg tggtaatcaa
    13261 gggcatcctt gtagcgcccc tgagcctcat taattactga aaggtatcgg taaacatcag
    13321 cttttgaaga ggtaaggttg tttttctgtg ctatgttaag tgcttggttt aggaagcgcg
    13381 atgatgtggg gtaatcgcca agttgaaagt ggcaacgcga tatgcttacc aaagcagcta
    13441 ttaaggttgg tatgtcgttg tattttgagg aaatgctttg gcactgggtg tagtagttta
    13501 tggcctggtt atagttccct ttctcatagt agtagtcgcc cagtccctgt aaactttgga
    13561 taattccaac ggtatcggaa gcattcaggc taatggtgta cgataggttg aagtactgat
    13621 aggcgctatc gtacttttgc gtaagcataa aaaccactcc aatttcattg agcgatttgc
    13681 tgtacgaagt tgaatcggat tgtttaatag cagcctcgct aaccttttta aagtaatcga
    13741 gactagttcc taaatcgcct gagtttttac tggcttggcc aagtagggtg taggtatcaa
    13801 tcagggcttt gtttttattg taaacagagg tagcttggtt gtttagcagg gttagggctt
    13861 caattccata ttgctttgcc tgcttaaagt tcttttggct taaatggagg ttgccgagct
    13921 taagtattag ctcaaggtaa acatttttat ttggaatatt ttttagtgtt tttatgcccg
    13981 cttcgagctg ttgtattgcc aggtcgagat tttggcttgc ttcgtaaacc ctagccattc
    14041 ccaaaagcga ttttgctgca aactcgggtt tattgattga tgagtaaagg ttggcagccc
    14101 ggttaaagta acggagcgag gtttcgtact cgccaagctt atagtttgct tcggcaagca
    14161 gctctagggc catcgattcc gtaaaggaat caccttcctt tttagccgtt acaagggctt
    14221 gtaacccaat atcaaggcag cgatcagggt tggtttttag cagggattta ccctgaacca
    14281 caaggtcagc ggcttttgag ctacccttta cagcaatctc ttggggcaaa gtatcggttt
    14341 ggcttaggcc aatttgcgct aaaccagcaa ggcttaatgt aagccatact attcgtagta
    14401 aatgtttcat ctaactttgc tgatgctaat atagctatat tccaaaaatt gcctactttt
    14461 atagccttga ataatttatg ctagtatgtt ttttaaagta aagaactacc tgtcgctggt
    14521 aaagttcagc catacaattt ttgcaatgcc ttttgccctt attggttatt ttttagctta
    14581 ctataagcac tcgctaaacc ctgattggtg gcttttttta aaggtgatac tctgcatggt
    14641 ttttgcccga aatgcagcta tgagtttcaa ccgtttagtt gataggcatt ttgataagtt
    14701 aaatccacgt acaagtgggc gggaaattcc taggggtatc attgctccca ggtctgttat
    14761 gcttttctgc attgtcaatt ccatcctttt cattttaact acattcttca ttaacccatt
    14821 ggtgttttac ctgtcgccta tagcgttggg tgtcatcctt ttttatagtt acaccaagcg
    14881 gtttaccatg ttcagccatc ttgttctggg aataggccta tcgcttgcgc caattggagc
    14941 atacctttcg gttactggta gttttgctct tattcctatt ctatactcgg ttgttgttct
    15001 tttctgggtg gcaggcttcg atatcattta tgctcttcag gatgaggagt ttgaccgtaa
    15061 ttccaagttg cactcaattc ctgctgcagt tggtgctcgc ggggctttgg tcattgcttt
    15121 ggtgttgcat tttatgagtg cattaatagt tatagccatt gggatactgg ctggctttaa
    15181 catttactac tggattggag cattcctttt tatagcttta atgatttatc agcattacct
    15241 tgtccgtcca accgatattt ctaaggtgaa catggccttt gccaccacca atggaatggc
    15301 aagccttctg ttcgcattgt ttaatataat ttcgatatta acttgctaag agttatgaaa
    15361 cacccatgga aggcagcttg cacaaacaca gatgaacaac cgagcgtttt ttgcgagctc
    15421 gacgaagtca aactatgggt gttccatccg ctcgataaag tttgttaaaa gcaagagagt
    15481 aacgagttag taattaatga tataaatatt ttgtactgat gaaaaaatat attgtattct
    15541 ttggtcttct atcactaata ttgggctgtt ccggtaacca aaatagtgct aacaaagtgg
    15601 agctggttga tggtcagcca gttattgatg taacggtcga ttcgttactt ttaaacgcta
    15661 agaattacga gagtaaggtg gttcgtgtta gcggtattgt tgagcatgtg tgtgttcata
    15721 ccggcttgcg gattacgctt caaggcaaat cgccggaaat gaagctgagt gtaaaaacat
    15781 cggatgccag tcggcctttt gatatgtcaa tgaaaggagc aaaggctacg gtcactggtg
    15841 ttttcaagag ggtaggggga gcaaacgagg tctgcgatgt ggaatcggct caccatccag
    15901 gtgaaattta ctttttggag tgtaacagca ttaatgttaa gtaaatatga cactttcaaa
    15961 gttcttgcgg gtaattcaca gggattgcag ctactttttt gccggaatgg ttttgctata
    16021 ctctgtaaca ggaattctct taaaccacgg gcatcagctt aacccctact actacatcga
    16081 tgttaaaaca gtaaaggttg atatccctag ggattcgtca aaggttaacg accagtttct
    16141 tttaggtgtc ttcagtccat ttggcgaaag cgagattagg tcgcattact accccgattc
    16201 atcaaccctg aaagtttttt tcaagaacgg aaccgccacg gtaaacctga atgagcaaac
    16261 cgcgctaatc gaaaatagta tacggcgacc aattctatat cagctcaaca tgatgcatcg
    16321 taaccgaccc aaggcatggt ggacatggtt tagcgatatt tttgccgttg ggctaatagc
    16381 tattacatta acggggcttt tcctgataaa ggggaaaaat ggaattaccc ggcgaggtgc
    16441 agtgctggtt gccgcaggcc ttattttgcc catactggcc atgatcattt tcttttaaac
    16501 gagagcaaat acaatacatt tacataaggg gggcttttgt cgttctaaaa tcaatgtcgt
    16561 ttagttaaca aaaatataca tgcctgtctt cctgagcgca gcgaaggatc tctaatcgac
    16621 cccatgagac gtttcgctcg cgctcaacat gacaatagct tcacgaacaa cgctcccagc
    16681 cccgtagggg cgaaacattt gtaactccat ggctactccc tcatgtgttg cgatgcacgc
    16741 aatacatttt ccaaagagca gagggaaagc gttgcatcgc aatagaagag cacggcaaag
    16801 agcggttttc caaaaaaaat acaagaatta aagggtttaa ggtaaagaga tgcttcggct
    16861 agctcagcat gacaagggtg cagaaaggaa gatagggttt atgtcatccc caacaaagtg
    16921 agggatctct tcaaaggatt gaagaaaatc agcaatgaga tacttcgcga agcctatccc
    16981 gataaatcgg gactcagcac gacagcatta tgtttcgctt gcgagcaata tgacaaataa
    17041 ctttaactaa ataacattga ttttaaaatc gttttcgatt tgtaattttt ttatttttgc
    17101 atgagttgaa aatctacg
//
LOCUS       DAOSAL010000055        16827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_2355_length_16827_cov_49.229609, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000055 DAOSAL010000000
VERSION     DAOSAL010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 16827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16827
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2355_length_16827_cov_49.229609"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1197)
                     /locus_tag="PK049_08215"
     CDS             complement(<1..1197)
                     /locus_tag="PK049_08215"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOK61581.1"
                     /translation="MMRSKTKNRLQTISILLLFITSSFSASHAQSADTLKISDAIKIG
                     LENNFSLRIARNEELVARNNNTLGAAGFFPKVDISSSASGSNYNIRQELATDEVNDTK
                     NYQTTSVYGGVQLSWTLFDGFAMFANRQRLSTLEGINSIAYQLKVEDVVSDIIVNYYT
                     LSAEKQLLDIYKEILRLSADRLNIAREKAAIGTSYLLAVMQAEVDYRTDSTQVLRQIN
                     RIKDLTITLNKLLGRDPSIQFEIESKIPEVKLYNQDSVITMLKSNNKELLISRLNLRL
                     KQIAIKEAQASRYPRLTLSSAYNFSQSNTPDGSTTVSRNIGPSYGVTLGVPLFNGFNV
                     NRNITNARIYMENQEFNIQALENDLTAEAVRLTNNLMLATNLVEIEKKSALLAKQNSD
                     ISIEKYR"
     gene            complement(1184..4246)
                     /locus_tag="PK049_08220"
     CDS             complement(1184..4246)
                     /locus_tag="PK049_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOK61582.1"
                     /translation="MNISTVSINRPVLASVVSIVIILFGVIGFTYLGVREYPSVDPPV
                     VTVSTSYVGANSAVIEAQITEPLEAQINGIDGIKNITSSSSDGRSSITVEFELGIDMD
                     NAANDVRDRVSRAIRNLPPDADPPIISKADANAETILTVTVQSTKRDLLELTDIANNV
                     VKERLQTIQGVSLVNIWGEKRYAMRLYLDPKKLAAFNVTPSDIRNALSRENVELPTGM
                     IEGYQTTLSIRTLGRLETPEEFNNLIIKYTNNVPVRVKDVGKADYAPENERTLLRGNG
                     LIPMVGVALTPQPGANYINIADEAYKRVEQLRKELPEDITLNYAYDSTINIRKAISEV
                     EETILLAFMLVVLVIFIFLRNWRTTLIPIIAIPISLIGTFFVMYLFGFTINILTLLGV
                     VLATGIVVDDAIVVMENIYSKIEHGMDNYKAGYTGSKEIFFAIISTTITLVAVFMPIV
                     FLQGITGRLFREFGVVVAAAVLISSIVSLTLTPMMSTRLLTRTQKEGKMMAAIGQGIS
                     WLSEYYGRSLQSFINRRWLAFVVMGISLFIIFIIGSRIPSELAPMEDKGRLSINVTAP
                     EGTSFEAIDEYIYDIASYVDTIPEKKAMIALSAPGWGGGTNRGFVRLLLTQPNERERS
                     QAQLADQISSYVRTKTFAQSYVIQDQTISTGRGGGLPVQFVVQAPNFEKLKQVVPEFL
                     ARAQADERFQVVDVNLKFNKPELVVEIDRDKARASGVTVRDVADALQLYFSGQRYGFF
                     IRNGKQYYVIGQADRPFRDDPDDLKNIYVRSTKGNLVDLGSLIRTSEQSIPPQRYRYN
                     RYISATFSASPAPGYTLGQGIDAMREISKEVLDDSFSTTLTGISQQFEESSNSLYFAF
                     LLALILVYLVLAAQFESFRDPLIIMFTVPLALAGAVLSLWMLGHTLNIFSQIGIIVLV
                     GIVTKNGILIVEFANQKKIAGMNKFEAVTFAAKQRLRPILMTSLATIFGTLPIALALG
                     AASTSRIPMGVTIIGGLLFALILTLYVIPALYTYVSTKKPNVIRHDEE"
     gene            complement(4274..5332)
                     /locus_tag="PK049_08225"
     CDS             complement(4274..5332)
                     /locus_tag="PK049_08225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOK61583.1"
                     /translation="MRRKIFTYHQLLLVLLTVASISCSKSDNGKKPSDGRSRQAIVVD
                     GIIVGYSEYERTITVPGTILPNEWVELRSEMAGKIVELNIAEGAMVRKGQLLARINDA
                     DIKALLQRRVIEEKLAADDEQRKKRMLEINAISVQEYETSLNKLEAIRADIAQSKAML
                     EKAEVRAPFEGRIGLRYVSPGAFVSVNTTIATLVQDNPLKLEFAIPERYASLVSNNME
                     VKFTAGDAQTQYPAKVYATDAQIDPETRSLKVRAIAENKSNQIIPGSYVRATLVFEKS
                     PRSILIPPRAIVPDMDIQNVFIYSQGKAKRIQVRLGERTGTTVEVIDGLNAGDSLIIS
                     GLTSVRDGMPVSINIKQN"
     gene            complement(5672..6157)
                     /locus_tag="PK049_08230"
     CDS             complement(5672..6157)
                     /locus_tag="PK049_08230"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOK61584.1"
                     /translation="MQGLTIRTEPVSKDIESIRNIVSSSGFFQDYEIPVAIELVEERL
                     EKGEASGYFFVFAEVNGETISYACFGPIPCTKNSYDLYWIATRNDFRGNGVGKVVLKE
                     AEKEIKRLGGRAIYIETSSKPLYKPTQKFYLDNGYRIEAILKDFYDLNDDKLIFTKQL
                     D"
     gene            complement(6161..7150)
                     /locus_tag="PK049_08235"
     CDS             complement(6161..7150)
                     /locus_tag="PK049_08235"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOK61585.1"
                     /translation="MNLKDLKAVILYNELSANAGPDEADVLDQVNVVSNALTTLGIMH
                     STLEFSLNLEKVQEKLIALKPDFVFNLVESVNNKGNLIFLAPALLNSLSIPFTGGSLE
                     TIFLTTSKTLTKELMSKASLPTPAWFHGKGKFTAEKGKKYIVKPIWEDGSLGLDENCV
                     FDATDPNLLKHIETLNFNTHFIEEYIDGREFNISILAGPKGPTVLPPAEIIFENYATT
                     KPKVVGYKAKWDESSYEYLHTFRTFDFNPSDDELIKKLQKICIDCWSAFNVNGYARVD
                     FRVDQNGNPYILEINVNPCISPDSGFYAACQKASIPFNEAINRIIHDIPSFKP"
     gene            complement(7147..8133)
                     /locus_tag="PK049_08240"
     CDS             complement(7147..8133)
                     /locus_tag="PK049_08240"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61586.1"
                     /translation="MLKVGLTYDLRSDYLKLGYSDEETAEFDSEETIEAIDNTLITLG
                     YKTQRIGNIFSLIDRINKGERWDLVFNICEGMHGIGREAQVPALLDAFNIPYVFSDPL
                     VLSLTLHKGQTKRVVRDCNIPTADFAIVESIQDIKHINLPYPLFAKPVAEGTGKGIDG
                     RSIITSNSDLQLVCQNLLARFQQPVLVETYLPGREFTVGIVGTGKNAKSIGVMEITIT
                     SKAKEQTYSFHTKENWKGVVEYTMASGEIEKICADTALKAWRCLGCRDGGRVDLKMDA
                     SGIPNFIEVNPLAGLNPSHSDLPMLARNNGIDYRQLIKLVVDSALIRLNGSV"
     gene            complement(8154..9422)
                     /locus_tag="PK049_08245"
     CDS             complement(8154..9422)
                     /locus_tag="PK049_08245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HOK61587.1"
                     /translation="MNGELEFEPLGQAEYCDDDEPPSGGKYDRYVDSANYLEVKNHSA
                     KAFPINDNTQSFIKKFYPGVTNEEWNSWRWQLKNSITSIKHLIKFIELTPQEIEAIDN
                     PHHSLPLRITPYYASLIGRDNETDPIRRCMVPTINELLLSKGESSDPLEEQHYSPVPN
                     LVHRYPDRVLFLTTGFCSAYCRYCTRSHMVAKDKCHVGVEAWQPALEYIRNHSEVRDV
                     LVSGGDPLTMSDSHIEYLLQNLRAIKHVEIIRIGTKVPVVLPQRITHNLLRILKQYHP
                     LYISIHFAHPAELTPETAEACARLANIGIPLGSQTVLLKGINDNVDTMKRLMQGLLKI
                     RVRPYYIYQCDPIPGSNHFRTKVSKGIEIIKGLRGFTTGYAVPTFVIDAPGGGGKIPL
                     LPEYFQGEIDGKVILKNYEDKTFYYPNPID"
     gene            complement(9829..10677)
                     /locus_tag="PK049_08250"
     CDS             complement(9829..10677)
                     /locus_tag="PK049_08250"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HOK61588.1"
                     /translation="MMTERIELYNNVCLECSKTTTKRYSTSFSLGTRLFDKPTRDAIY
                     AIYGFVRFADEIVDSFHPYNKAVLLNRFKTETYKAIEEGISINPILQSFQLVVNKYSI
                     DQHLIDAFLQSMEMDLNPTIYDRDKISEYIYGSAEVVGLMCLKVFCRGNQQKYEKLYQ
                     PARRLGAAFQKINFLRDMGEDFNDLGRVYFPNVDFNNFTPEKKLEIETDIQSDFDAAL
                     EGIKELESNSKLGVYVAYRYYLSLFHKIKRIPPHKLLGKRFRISNTKKALLMASSWAG
                     VKIGVI"
     gene            complement(10658..12145)
                     /gene="crtI"
                     /locus_tag="PK049_08255"
     CDS             complement(10658..12145)
                     /gene="crtI"
                     /locus_tag="PK049_08255"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HOK61589.1"
                     /translation="MARKVVIVGTGLGGLSTALRLTTLGFEVEMIEKYHQPGGRLNKL
                     QHNGYTWDMAPTFFSMSYEFEELIDFCGIERPFDFFELDPLYKVNFSNSKKFFTIHKD
                     IHKLAREFEGIEDDFAEKMYQYLEKTGKLFHDVENRIVRRNFNSLLSYALGLASVPWG
                     HAPLMVKSMWDELNRRFTSYEVKVIFSLVAFFLGATPFDTPAVYSILTYTELKHDGYH
                     NVRGGMYKIVDGIVDILSKRGVKFHYNTEIVDFKYNNKKLTGVVDQNGNIWGADIFVV
                     NADAAWFRGAILKRKAYSENKLDRMKWTLAPFTVYLGVKGKIDGLSVHNYFLGTNFKE
                     YSSKIFKNQVSLEKPYYYVNVPSKANPEFAPGSNESLFILCPVPDLRYKPDWTDSHEL
                     AQNIIVDLSERVNYDIAGNVETQIIYNPAHWQKMFNLYKGSGLGLAHDLNQIAYMRPH
                     NTDEIFKNLFYVGSSTIPGTGLPMAVISSKLTVEQIVKRYDDGKN"
     gene            12252..13706
                     /gene="crtI"
                     /locus_tag="PK049_08260"
     CDS             12252..13706
                     /gene="crtI"
                     /locus_tag="PK049_08260"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HOK61590.1"
                     /translation="MQTVSTVGAGIGGIATAIRLARKGYRVKVFEQQPNAGGKLNELR
                     LSGFRFDAGPSLFTLPQLVDELIGSRDYSIFSYQKLELITRYFFPDGTVLDAWAEPER
                     FAEEVSQKFNDKHTAVLRYLKESRELYELTANTFVYSPFPQLKNFFTLQSLKLAMNPK
                     KLKAFETMHEVNCKHFSDHRTIQLFDRYATYNGSNPYLAPGTLTMIPHLEHNIGAYFP
                     TAGMYAITTEMVKIAQNLGVEFEYNAHVDKVDIRNGRVVGIKVNGSSAESNYVVNNTD
                     IFTAYKTLLHEVPLASRLAAQQPSSSALIFYFGVKGIHPKLDVHNILFTSDYYSEFKA
                     MEQGSIFNDPTIYIFITSKRIAADAPDGHENWFVMINVPPNTGQDWDELRLKAREAIL
                     VKIKRMLGIDLASLIVAEDYLDPVRIEKRTGSFRGSLYGISSNNRMAAFNRHPNRSRN
                     VKGLYFVGGSVHPGGGIPLCLASAKIVDTYFKPL"
     gene            13708..14346
                     /locus_tag="PK049_08265"
     CDS             13708..14346
                     /locus_tag="PK049_08265"
                     /inference="COORDINATES: protein motif:HMM:NF016155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HOK61591.1"
                     /translation="MNRLAGYFRVIIPIAYLIGIVGISIKINNPQYFKVTSIFVPVSF
                     LILMLFHKPHSLRFWITLASIGLLGFLVEAIGTNTGLIFGQYTYGSPLGFGVFGTPLN
                     MAINWMMLVYLVAFVLQKWNINITLKSGVSAIILTLFDWVLEPVAIRLDMWRWDLQTP
                     PFHNYIGWFLFSFIVFSIFYRFATKLHNPLANTFAVMHSLFFIVLNLIFRLI"
     gene            14360..14983
                     /locus_tag="PK049_08270"
     CDS             14360..14983
                     /locus_tag="PK049_08270"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOK61592.1"
                     /translation="MIKPAHNALYERFFNAYFNYILRRNFREIRIEGDFVPDNRPILL
                     VPNHFSWWDGFFAWKLNTMLLHKKFYLMMLESELSRHRFFAKLGAFSIAPTSKGMLES
                     LRFATEVIKQPNNLLVFYPQGKLYSQHHMALEFQKGIERIVEQSDNFRLLMCANLIDY
                     YAYPKPSLTIYLQEYERLQDFSLTQFQLTYNLFLKQSIHKQDKIHRQ"
     gene            14980..16077
                     /locus_tag="PK049_08275"
     CDS             14980..16077
                     /locus_tag="PK049_08275"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOK61593.1"
                     /translation="MILFYLVLPIVIFAIIRFLVALFNYFTQPYLQSYPIISSETVSV
                     LIPARNEEANIQNLLADLQSQSYTNIEVIVYNDLSTDATGKIVEQFVKADNRFRLING
                     SGLPEGWLGKNNACHQLALQANGNYLLFLDADVRVEKSFVGQALAYMQHKKLHLLSFF
                     PYQVLGSFGEAITVPVMQWILLSLLPLKLVLWSRRRSLSAANGQMMMFKAETYRKHRF
                     HEIYRSNPVEDILIAREMKRFRYRISTLLGTPGDIRCRMYASGSDAIEGFSKNVCEFF
                     GGNRNVMFSFAAITTLGPFLVMFFMPFPLVFTYFFSVIMARLFIADLSKQNVFKAVIL
                     FPYQQISFLQMVIRWEKNRKRGTLIWKGRKI"
     gene            16088..>16827
                     /locus_tag="PK049_08280"
     CDS             16088..>16827
                     /locus_tag="PK049_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61594.1"
                     /translation="MAKGLLLVASFSILLLNQQPLIANINDDFDHRLYVAFISGNMAD
                     WGKVLSELEAIASKNPSATNRFRLLHAQYGYIGYLLGVNKTSIAKQLIDKAEKQANIL
                     ASIPTYQSTAKAMQAALLAYRISISPYKAPFLGPKSASLIDEALALNPTNYFALIEKG
                     NARHYAPSIVGGNPTEAVELYRKAIELIEKENPASKPQKWWYINTYTQMGLAAQKAGN
                     KDLAKKIYLTILGMEPNYKWVREELLPKV"
BASE COUNT         4683 a         3492 c         3550 g         5102 t
ORIGIN      
        1 acgatacttc tcaatggaga tatcggagtt ttgctttgcc agtagtgccg attttttttc
       61 aatctctacc aggtttgttg ccagcatcag attattcgta agtcttacgg cttcggcagt
      121 taaatcattc tccaaagcct gtatattaaa ttcctgattc tccatgtaga tgcgagcatt
      181 ggtaatgttc ctgtttacat tgaacccgtt gaatagtgga acgccaagtg taactccgta
      241 cgaaggtcct atgttccggg aaacggtggt tgacccatca ggtgtgttgc tttgactaaa
      301 attataagcg gatgagagag taagacgagg gtatcgtgag gcttgagctt ccttaatggc
      361 aatttgcttt aaacgtaaat ttaaccgtga tataagcaac tccttgttgt tgcttttgag
      421 catggtaatt actgaatcct gattgtaaag ctttacttca gggattttac tttcaatttc
      481 aaattgaata gatggatcgc gcccaagcag cttgttcagg gtaatggtaa ggtctttaat
      541 cctgttaatt tgccgtaaca cttgagttga atcggttctg taatcaacct cagcttgcat
      601 aacggcaagc aggtagctgg taccaatggc tgctttttcg cgggcaatat taagcctatc
      661 ggctgaaagg cgcaaaatct ctttataaat atcgagcagc tgcttttcgg ccgaaagggt
      721 atagtagtta acaataatat ccgatactac atcctcaacc ttaagctgat aagcgatgct
      781 gtttattccc tccagtgtac ttagtcgctg ccggttggca aacatggcaa aaccatcaaa
      841 gagtgtccat gatagctgaa cccctccata aactgaagtg gtttgatagt tttttgtatc
      901 gttaacctcg tcggttgcca gttcctggcg tatattatag ttgcttccgc tggccgagga
      961 actaatatct actttaggaa agaatcctgc tgcccctaag gtgttgttgt ttcgagcaac
     1021 tagttcctcg tttcgggcta ttctaaggga gaagttgttc tccaacccaa tcttaatggc
     1081 atcggaaatt ttgagcgtat cagctgattg tgcatgtgag gctgaaaatg agcttgtaat
     1141 aaacaaaagc aatatcgata tagtctgtaa tctatttttt gttttactcc tcatcatggc
     1201 ggataacatt tggttttttg gttgaaacat aggtgtaaag tgctggtatt acgtatagcg
     1261 ttagaataag agcaaagagt aatcctccaa taatggtaac ccccattggg atgcggcttg
     1321 ttgatgcggc tcctaatgct aaggcaatgg gcaatgttcc gaaaatggtg gcaaggctgg
     1381 tcatcagaat gggacgtagc ctctgcttag cggcaaaggt aactgcttca aatttattca
     1441 tgcccgcaat ctttttctgg ttggcgaatt caacaatcag aataccattt ttggtaacaa
     1501 ttccaaccag cacaattatt ccaatctggc tgaaaatgtt aagggtgtgt cctaacatcc
     1561 acaacgacag aactgcccca gccaaagcga gtggaactgt aaacataatg ataagtgggt
     1621 cacgaaaact ttcgaactgt gctgctaaaa ccaggtaaac aagaattagc gcaagcagaa
     1681 atgcaaagta taggctgttg gagctttcct caaactgctg cgagataccg gttaaagtag
     1741 tggaaaaact atcgtcgagt acttccttgg aaatctcgcg catggcatcg atgccttgcc
     1801 ctaatgtata cccaggtgca ggcgatgcgc tgaatgtagc cgaaatgtaa cggttatacc
     1861 tgtaacgctg tggaggaata ctctgctcgc tagttctgat tagcgaacct aaatcgacaa
     1921 ggtttccctt tgtgctccgc acgtatatgt tttttaagtc atcgggatca tctcggaatg
     1981 gtctgtcggc ttggcctatt acgtagtact gcttcccgtt ccgaataaag aaaccgtagc
     2041 gctgtccgct gaagtaaagc tgaagggcat cggcaacatc ccttacggta acacctgagg
     2101 ctcttgcctt atcgcggtca atttcaacaa caagctcagg tttattaaac ttaaggttga
     2161 catcaaccac ctggaagcgt tcatcggcct gtgcccgggc taaaaattcg ggtacaacct
     2221 gctttagctt ttcaaagttt ggtgcctgca ccacaaactg aaccggtaaa ccaccacccc
     2281 ttccggtgct gatggtttga tcctgtatta catacgactg ggcaaaggtt ttagtgcgga
     2341 cgtatgatga tatttgatcg gcaagttgcg cctgtgaacg ttcacgttca ttgggctgag
     2401 taagcagtaa ccgaacaaat cctctatttg tgccacctcc ccaaccggga gccgatagag
     2461 caatcatggc ctttttctca gggattgtat caacatacga ggcgatatcg taaatgtact
     2521 cgtcaatagc ctcaaacgaa gtaccctcag gtgcagtaac atttatcgat agtctgcctt
     2581 tgtcctccat tggagccaac tccgaaggga tacggctgcc tataataaaa ataatgaata
     2641 gtgagatgcc cataaccaca aatgccaacc acctgcggtt tatgaatgat tgtaacgatc
     2701 ttccgtagta ctccgatagc cagctgattc cctggccaat ggcagccatc atttttcctt
     2761 ccttttgcgt tctggtcaaa aggcgagtgc tcatcatcgg ggtaagcgta agcgatacaa
     2821 ttgacgaaat tagtacagcg gctgccacaa ctactccaaa ctcgcggaaa agacgacctg
     2881 tgatgccttg tagaaataca attggcatga ataccgcaac cagagtgata gttgtggaga
     2941 taatggcaaa gaaaatctcc ttgctacctg tgtatcctgc tttatagttg tccatgccat
     3001 gctcaatttt ggagtagatg ttctccataa caacaatggc atcgtcaact acaataccag
     3061 tggcaagaac tacacctaat agagttagaa tatttatggt aaaaccaaaa aggtacatca
     3121 caaaaaaggt tcctattaac gaaatgggaa tggcaataat tggaattaag gtagtgcgcc
     3181 agtttcgcag aaaaataaaa ataacaagaa caacgagcat aaacgccaga agaatggttt
     3241 cctcaacctc agaaattgcc ttgcggatat ttattgttga atcgtaggcg taatttaggg
     3301 tgatatcctc gggcaattcc ttacggagct gctcaacacg cttgtaagcc tcatcggcaa
     3361 tgttgatgta gtttgcaccg ggctgtgggg ttagcgcaac accaaccata gggatcagcc
     3421 cattaccacg gagtagcgtt cgttcattct ctggggcata atcggctttg cctacatcct
     3481 taaccctaac gggcacgtta ttggtgtatt tgatgatcag gttgttaaac tcctcgggtg
     3541 tttcgagtcg accgagggtt cggattgaaa gagtggtttg gtaaccctca atcataccgg
     3601 taggaagttc aacgttctcg cgcgaaagcg cattgcgaat atcggaaggt gttacattga
     3661 acgcagccaa ctttttcggg tcgaggtata atcgcatggc gtagcgtttt tcgccccata
     3721 tatttaccag actgacgccc tgaatggttt gcaatcgttc cttaacaaca ttattggcaa
     3781 tgtcagttag ttcaagtaaa tcgcgtttgg tgctttgaac cgtaacggtc agaatggttt
     3841 ccgcattggc gtcagctttg cttataatgg gcgggtcagc gtcgggggga aggttacgaa
     3901 tggcacgcga aacacggtcg cgcacatcgt tggcggcatt atccatatca atccccagtt
     3961 caaactcaac ggtgatacta ctacgaccat cggagcttga tgatgtaatg ttttttatac
     4021 cgtcaatccc atttatttga gcctcaagag gctcagtgat ttgtgcctca ataactgctg
     4081 agttagcccc aacatacgat gtggaaaccg taaccacggg tgggtctaca ctggggtatt
     4141 ccctaacgcc gaggtaagta aagcctatta caccaaaaag aataattact attgaaacca
     4201 ccgatgccag aactggccta tttatgctaa ctgttgagat gttcactttg gtaaccttgt
     4261 ttagttaaaa cttttagttt tgcttgatat taatgctaac aggcataccg tctcgcacag
     4321 aggtcaaacc cgatattatt aaactatcgc cggcatttaa gccatcaatt acctcaactg
     4381 tagtacctgt tcgttcaccc aaccttacct gaatgcgctt agctttaccc tgggagtaaa
     4441 taaaaacatt ttggatatcc atgtcgggaa caattgccct aggcggtata agtattgaac
     4501 gaggactttt ctcaaaaacc agggttgctc tgacgtagga gccaggaatt atttgattac
     4561 tcttattttc ggcaattgcc ctaaccttaa gcgagcgtgt ttcagggtct atttgggcat
     4621 cggtagcgta aacctttgca gggtattgtg tttgtgcatc accggctgta aactttacct
     4681 ccatgttatt ggataccaaa ctagcgtaac gctccggtat ggcaaattcc aactttaatg
     4741 ggttatcctg aaccagtgtg gcaatggttg tgtttaccga tacaaatgca ccaggactaa
     4801 cgtagcgcaa gcctattcgt ccttcgaagg gagcacgcac ttcagctttt tccaacatcg
     4861 ctttcgattg ggctatatcg gccctgatgg cttcaagctt attaagcgat gtttcatact
     4921 cctgaacact tatggcattt atctcgagca tgcgcttttt gcgttgctca tcgtcggcgg
     4981 caagcttctc ctcaattacc cttcgttgga gtagcgcttt aatatcggca tcgttaatcc
     5041 gtgcaagtag ttgccctttg cgtaccattg ctccctcagc tatgtttagc tcaacaattt
     5101 ttcctgccat ttcggagcgt agctcaaccc actcgtttgg gagaatggtg ccaggaaccg
     5161 tgatggtcct ttcatattca gaatatccaa ctataattcc atcaactact attgcctgcc
     5221 ggctgcgacc atcgctaggc ttttttccat tatccgattt actgcacgat atgcttgcca
     5281 ctgtaagtaa tacaagcaaa agttgatggt atgtaaaaat ttttcttctc atttccgttt
     5341 atttatttct gaaattaatg actaattttt atctaaaagg attaacccaa aaaaatgttt
     5401 taaaattttc aacttgactc atattttgtt tggcataata gcttaattaa ataaagattt
     5461 tgttgaactg aaagggttaa aacccgatat ccttgagttt actgatacat cacctttaaa
     5521 gggttcaaac acgtacggga ttgtagtgat gaccagcgac ggtggtcaat cggaaatggt
     5581 agtacagaaa gttgtggttc cggattaaag ggtatgaata ctaagccccg ctttctgcgg
     5641 ggcttttcat ttgttttaat ggctaacaaa atcagtcaag ttgctttgtg aaaattagct
     5701 tatcgtcatt taaatcgtag aaatccttta gtatggcttc aatcctgtag ccgttgtcaa
     5761 ggtagaattt ttgggttggt ttataaagtg gcttcgagga agtttcaata tatatggctc
     5821 ttccgccaag tcttttgatt tccttttcgg cttccttcag tactactttg ccaactccat
     5881 tcccccgaaa atcgtttctg gttgcaatcc agtaaaggtc gtaactgttt tttgtgcagg
     5941 gaattggacc aaagcaggca tacgaaatgg tttcgccatt cacctcggca aaaacaaaga
     6001 agtaaccact tgcttcacct ttttctaatc gttcttccac taactcaata gcaacaggaa
     6061 tctcataatc ctgaaaaaat ccggatgaac tgacaatgtt tctgatggat tcaatatcct
     6121 tggataccgg ctcagttctg atagttagtc cttgcatggc ttacggctta aacgatggaa
     6181 tatcatgtat tattctattt attgcttcgt taaaaggtat tgatgccttt tggcatgctg
     6241 cataaaatcc actatcgggc gaaatgcatg ggtttacatt gatttcaagg atgtaaggat
     6301 ttccattttg gtcaaccctg aaatccacac gggcatatcc attaacgtta aaggcactcc
     6361 agcaatcaat acatattttt tgtagtttct taattagctc atcatcgctg ggattaaaat
     6421 cgaatgtcct aaaggtgtga aggtattcat agctactttc gtcccatttg gctttataac
     6481 caactacttt aggtttagtt gtcgcatagt tttcgaagat aatttcggcg gggggtagaa
     6541 cggtagggcc cttggggcct gccagaatgg aaatgttaaa ctcacgccca tcgatatact
     6601 cctcaataaa gtgtgtgtta aagtttagtg tttcaatatg ttttaatagg ttgggatcgg
     6661 ttgcatcaaa aacgcagttt tcgtcaagtc caagcgaacc atcctcccaa attggtttta
     6721 caatatattt tttccctttt tctgccgtaa actttccttt gccatgaaac caagcaggtg
     6781 ttggtaggct tgccttgctc attagttcct tggtaagggt ttttgaggta gtcagaaaaa
     6841 tggtttccag tgagccccct gtaaatggta ttgaaagtga atttaatagg gctggggcaa
     6901 gaaaaataag attcccttta ttgttaactg attcaacaag gttaaacaca aaatcgggct
     6961 tcaacgctat cagtttttcc tgaacttttt caaggttaag tgaaaactct agcgtagaat
     7021 gcataatacc caaggtggtt agagcattgg aaactacatt tacttggtct aggacatcgg
     7081 cttcatcggg gccagcgttt gccgaaagct cgttgtatag gataactgct ttaagatctt
     7141 taagattcat acactaccat ttaatcttat caatgcagaa tcaactacaa gcttgatgag
     7201 ttgcctatag tcaattccat tattacgggc gagcataggt aaatcggagt gcgaagggtt
     7261 taaccctgcc aaagggttaa cctcaataaa atttggaata cctgaagcat ccattttcag
     7321 gtcaacccgt ccaccatccc tgcaccctaa gcagcgccat gcttttagtg ctgtatccgc
     7381 acatatcttt tcaatttcac ccgaagccat ggtgtattca actacgccct tccagttctc
     7441 cttggtatga aacgagtagg tttgttcctt ggcttttgaa gtaatggtta tttccattac
     7501 tccaattgat tttgcatttt tcccagtgcc tacaatccct acggtaaatt cccttccggg
     7561 taagtatgtt tccaccagca ccggttgttg aaatcttgct aaaagatttt ggcaaaccag
     7621 ttgcaaatcg gaatttgagg taataattga cctgccatca atacctttac ccgttccctc
     7681 ggcaactggt ttggcaaaaa gagggtaggg taaattgata tgcttaatat cctgaattga
     7741 ttcaacaatg gcaaaatcgg cagttggaat gttacaatcc cttacaacgc gttttgtttg
     7801 tcccttatga agtgtgagcg acagaactaa cggatcggaa aaaacgtaag gaatattaaa
     7861 ggcatcgagc aatgccggaa cttgagcctc acgaccaatt ccatgcatac cctcacaaat
     7921 attaaatact aaatcccacc gctcgccttt attaatccta tcgataagag aaaaaatatt
     7981 gccaatacgt tgagttttat accccagtgt gattagtgta ttgtctattg cttcaattgt
     8041 ttcttccgag tcaaattcag cggtttcttc atcggaatag ccaagcttga ggtaatcgct
     8101 acgtaaatcg taggttaatc ccacttttaa cataatgcca gattaatcag tttttaatca
     8161 attgggttgg ggtagtagaa ggttttatcc tcatagttct tcaaaatcac cttgccatca
     8221 atttcgccct ggaagtattc cggtaacaaa ggaatttttc cacctccacc gggggcatca
     8281 ataacaaagg tcggcacagc atagccagtt gtgaatccac gcaaaccttt gattatttca
     8341 atcccttttg agacttttgt tctaaagtga ttcgatccgg gaatggggtc gcattggtaa
     8401 atgtagtaag gacggaccct aattttaaga aggccctgca ttaatcgttt catggtatca
     8461 acgttgtcgt taataccttt tagtaatacg gtttgacttc ccaagggtat tccaatattt
     8521 gccaatcgtg cacaagcctc agctgtttcg ggggtaagtt cggctgggtg tgcaaaatgg
     8581 atactgatgt aaagtggatg gtattgcttg agtatccgta gtaagttatg tgttattcgc
     8641 tggggcaaaa ctacgggcac tttggtgcca attcggatga tctcaacatg cttaattgcc
     8701 ctaaggttct gcaataggta ttcgatgtgg gaatcgctca tggttaaagg atcaccaccc
     8761 gatactagaa catctctaac ctcgctatgg tttctgatat actcaagggc aggttgccat
     8821 gcttcaaccc caacatggca cttgtccttt gcaaccatat gcgaacgggt gcagtatcgg
     8881 cagtaagcag agcaaaagcc ggtggttagg aataagaccc tatcgggata gcgatgtacc
     8941 agatttggca cagggctata gtgttgttct tccagcggat ccgatgattc acctttggaa
     9001 agtaatagtt cgtttatggt tggaaccata catcgcctaa taggatccgt ttcgttatct
     9061 ctacctatta agcttgcata gtatggggtt atccttaaag gtaatgagtg atgcggatta
     9121 tcaatagcct caatttcttg cggtgttagc tctataaatt tgataagatg ctttatcgat
     9181 gttatgctat ttttaagctg ccatcgccag ctattccatt cctcgttagt tactccggga
     9241 taaaattttt taataaaact ttgagtgtta tcatttatag ggaacgcttt agcagaatga
     9301 tttttgacct ctaaataatt agcagaatct acatacctgt catatttacc accacttgga
     9361 ggttcgtcat cgtcgcagta ttctgcttgc ccaagcggct cgaattctag ttcaccattc
     9421 atagttttag tctttaaagt gttgttatcg aattacttag ccttgatttc ctatagccaa
     9481 attatattca aatattggaa tttgttaaat acgatgtttg cccttgaagg ctcttatgta
     9541 aatgtcagta ataaaaaatg ttattttaag cgtaagactt atgctaaagt tatgataaac
     9601 agttagttgt gtatttggtt tgttgataaa aaaatattaa caggcctata ggatttatga
     9661 acagttggcg aatacttggg gcgacattaa atttttagat agctaaaaag gttacttaag
     9721 tgaatcaaat ctatttttgt gcaagaccta tagacttttg attatacttc aaaattaaaa
     9781 aaattaaaaa agcacataaa gccccatagg attagtgaaa gtagttcatt aaatcactcc
     9841 aattttaacc cctgcccagc tgcttgccat gagtaatgcc ttttttgtgt tgctaatacg
     9901 gaaacgttta ccaagcaact tgtgaggagg aatccgttta atcttgtgga agagcgagag
     9961 gtagtatcgg taagctacgt aaacacctag cttggaattg ctttcaagct ctttgattcc
    10021 ttcaagggca gcatcaaaat ccgattggat atcggtttca atttctagtt ttttttcagg
    10081 tgtaaagttg ttgaaatcaa cattggggaa gtaaacccta cccagatcgt taaaatcctc
    10141 gcccatgtcg cgaaggaaat tgattttttg gaatgcagcg ccaagcctcc gggctggttg
    10201 gtaaagcttt tcatacttct gctggttgcc cctgcaaaaa acttttaagc acataagccc
    10261 taccacttcg gctgagccat aaatatactc acttatttta tccctatcgt agatagttgg
    10321 gttaaggtcc atttccatgc tttgcaaaaa ggcatctatc aggtgttggt caatgctgta
    10381 tttatttacc actagctgaa agctttgtag tattgggttt attgagatac cttcctcgat
    10441 tgctttgtag gtttcggttt taaagcgatt aaggagtaca gccttattgt aggggtggaa
    10501 tgagtccaca atctcatcgg caaaacgaac aaaaccgtat atggcataaa tggcatcgcg
    10561 ggtaggttta tcaaaaaggc gtgtgcccaa ggaaaaactt gtgctgtagc gtttggttgt
    10621 tgttttgcta cactcaaggc aaacattatt gtatagttca attctttccg tcatcatacc
    10681 gttttacaat ttgctcaact gttaattttg aactaataac tgccattggc aagcccgttc
    10741 cgggaatggt gcttgaaccc acatagaata ggtttttgaa aatttcatcg gtattgtggg
    10801 gtcgcatgta agctatttgg tttaaatcgt gggcaaggcc aagaccgctg cccttgtaca
    10861 ggttaaacat tttttgccag tgcgcagggt tgtaaataat ttgcgtttca acatttccgg
    10921 caatatcgta attaaccctc tcgctgaggt caactattat attttgggct aactcatggc
    10981 tatcggtcca atcgggttta tagcgtaagt cgggaacagg gcaaagaata aaaaggcttt
    11041 cgttactacc aggggcaaac tcagggtttg ctttagatgg gacattaacg tagtagtaag
    11101 gtttttctaa gctaacctga ttcttaaaaa tcttggagct gtattcctta aagttagttc
    11161 ccaaaaagta gttgtgaaca cttaaaccat caattttacc ttttactccc aggtaaacag
    11221 taaaaggggc aagagtccat ttcattctat cgagcttgtt ctcgctgtag gctttacgct
    11281 taagtattgc acccctaaac catgctgcat cggcatttac aacaaaaatg tcagcacccc
    11341 atatgttgcc attttggtca acaacaccgg ttagtttttt gttgttgtat ttaaaatcaa
    11401 ctatctcggt gttgtagtgg aactttacac cccgctttga gagaatatca acaatgccat
    11461 caacaatctt atacatacca ccgcgaacat tatggtatcc atcgtgcttc agttcagtat
    11521 aggtaagaat ggagtaaacc gcaggggtat cgaatggtgt tgcccccagg aaaaatgcca
    11581 ccaatgagaa aatcactttc acctcgtagc tggtaaacct tcggttaagc tcatcccaca
    11641 tcgatttaac cattagagga gcatgccccc acggtacgga ggctagccct aatgcgtatg
    11701 aaagcaagct gttgaagttg cggcgcacaa ttctgttttc cacatcgtgg aatagcttac
    11761 ctgttttttc caggtattgg tacatttttt ctgcaaaatc atcttcaatt ccttcaaact
    11821 ccctggccag cttgtgaata tctttatgta ttgtaaaaaa ctttttggaa ttgctaaaat
    11881 ttaccttgta gagcgggtcg agctcaaaaa agtcgaaagg tctttctatt ccacaaaaat
    11941 caataagttc ctcaaattca tagctcatgg aaaagaaagt tggcgccata tcccaggtgt
    12001 agccattgtg ttgcagctta tttaaacggc ctccgggttg atggtacttt tcaatcattt
    12061 ccacttcaaa accaagcgtt gttagcctaa gtgcagttga taaaccacca agccctgtcc
    12121 caacaattac aactttcctt gccataaatg cttatttact atttaaaaca cattaacaaa
    12181 cattaaatat acaattttaa ttaaatttac catgagtatt aaccagaaat gccttatttt
    12241 gtttaaaaag tatgcaaacg gtttcaacag taggggcggg aattggcgga attgccacag
    12301 caatccgatt ggcacgcaag gggtataggg ttaaagtgtt tgaacagcaa cccaatgctg
    12361 gtggtaaatt gaatgagcta agactttcag gttttcggtt cgatgcgggg ccatcgttat
    12421 ttactttacc acagctggtg gatgaactta tcggaagcag ggactattcc attttcagtt
    12481 accaaaaact tgagctaatc acccgttact tttttcccga tggcactgtc cttgatgcat
    12541 gggctgagcc tgaacgcttt gccgaagagg tatcccaaaa gtttaacgat aagcatacgg
    12601 cagttctccg ttacttaaag gagagtagag agctttacga acttacagcc aatacttttg
    12661 tgtatagtcc tttccctcag ctaaagaact tttttaccct gcaatcgctt aaacttgcca
    12721 tgaatccaaa aaagctgaaa gcgttcgaga ccatgcatga ggtaaactgt aagcactttt
    12781 ctgatcaccg aacaatccaa ctctttgata ggtatgccac atacaatggc tcaaacccat
    12841 accttgcacc cggaacgctt accatgatac cccaccttga gcataatatt ggagcttact
    12901 ttcctacagc agggatgtat gccataacca ccgaaatggt taaaattgcc caaaacctgg
    12961 gtgttgaatt tgaatataac gctcatgtgg ataaggttga tatccgaaat ggccgagtgg
    13021 taggcattaa ggtaaatgga agtagcgctg aaagcaacta tgtggtcaat aacaccgata
    13081 ttttcacggc atacaaaacg cttttacacg aggttccttt agccagccga ttggctgcac
    13141 agcagccatc atcatcggca ttaatctttt actttggggt taagggtata caccctaagc
    13201 tcgatgtaca taatatcctc tttacgtccg attactactc ggaatttaag gccatggagc
    13261 aaggttcaat tttcaacgac ccaaccattt acatatttat cacttcaaaa cgtattgccg
    13321 ctgatgctcc cgatgggcat gagaactggt ttgtgatgat aaatgtacca ccaaatactg
    13381 ggcaggactg ggatgaacta aggctcaaag ctcgcgaggc catacttgta aagattaaac
    13441 gtatgttagg tattgatttg gctagtttaa ttgtagccga ggactatttg gatcccgttc
    13501 ggattgagaa acgtaccggg tcgttccgcg gttcgcttta tggaattagt tccaataaca
    13561 gaatggcagc gttcaaccgc catccaaaca gaagtagaaa tgttaaaggt ctttattttg
    13621 ttggcggaag cgttcaccca ggcggaggaa ttccgctttg cctagcttcg gccaagattg
    13681 ttgataccta tttcaaaccc ttgtagcatg aatcggcttg ctggatattt tagggtgata
    13741 atccccatag cataccttat tggcattgtg ggtatctcaa taaagataaa taatccacaa
    13801 tactttaagg ttacttccat ttttgttcca gtatcgtttt tgatactaat gttattccac
    13861 aaacctcata gtttaaggtt ttggattact ttggcatcga ttggcttact gggtttttta
    13921 gttgaggcta ttggaactaa cacgggtctg atttttggcc aatacacgta tggcagtcct
    13981 ttaggttttg gagtgtttgg aacacccctg aatatggcca ttaactggat gatgcttgtt
    14041 taccttgttg cctttgtgtt gcagaaatgg aacattaaca taactctgaa atcgggggta
    14101 tcagccatca tcctaacgtt gtttgactgg gtgcttgaac cggtagccat ccggctcgat
    14161 atgtggcggt gggatttaca aacaccaccc tttcataact atatcggttg gtttttattc
    14221 tcgttcatag tattcagcat cttttaccgg tttgcaacaa aattgcataa cccgcttgcg
    14281 aatacttttg cggtaatgca tagtttgttt tttatcgttc ttaaccttat ttttaggttg
    14341 atttaaattt ggtatcgaag tgattaaacc ggcacataac gcattgtatg agagattctt
    14401 caatgcttac tttaactata ttctgagacg taatttcagg gaaatccgaa ttgagggcga
    14461 ttttgttcct gataatcgcc caattttact ggtaccaaac cattttagct ggtgggatgg
    14521 tttttttgca tggaagctta ataccatgct gctacacaaa aagttttatc tgatgatgct
    14581 tgaaagtgag cttagcaggc atcgtttttt tgcaaagctg ggggcttttt ccattgctcc
    14641 cacatcaaaa ggaatgcttg agagcttaag gtttgccact gaggttataa aacaacccaa
    14701 taacctttta gtattttatc ctcaaggcaa actgtatagc cagcatcaca tggcacttga
    14761 gttccagaag ggtattgaac gtatagtgga gcaatcggac aacttcaggc tgctgatgtg
    14821 cgctaatctt attgactact acgcctaccc aaaaccatcg ctaaccatat atttacaaga
    14881 gtatgagcgg ctacaggatt ttagcttaac tcaattccag cttacctata atctattcct
    14941 aaaacagagc attcacaaac aggacaaaat acacagacaa tgatactgtt ttacctagtg
    15001 cttcctattg taatttttgc aattattcgg tttttggttg cgcttttcaa ctactttact
    15061 caaccctacc tgcagagcta tccgataata tcgtccgaaa ccgtttcggt gcttattcct
    15121 gcacgcaatg aggaggcaaa tattcaaaac ctgcttgccg atttgcaatc gcaaagctat
    15181 accaatattg aggtaattgt ttacaacgat ttatcgaccg atgccaccgg taaaattgtt
    15241 gagcagtttg tgaaagccga taaccgattt aggttgataa acggcagtgg tttgccggag
    15301 ggttggctgg ggaaaaataa tgcatgtcat cagcttgcct tacaagcaaa tggcaattac
    15361 cttttattcc ttgatgccga tgttagggtg gagaaaagtt ttgtggggca agccttggct
    15421 tacatgcagc ataagaaact tcatctacta tcgtttttcc cataccaggt gctaggctca
    15481 tttggtgagg ccatcactgt tcctgtaatg cagtggattt tactgtcgtt attgccctta
    15541 aagctcgttt tgtggagccg tcgtcggtca ctttcggcag ccaacggaca aatgatgatg
    15601 tttaaggcag aaacctaccg taaacatcgt ttccatgaaa tatatagaag caatccggtg
    15661 gaggatattc taattgccag agagatgaaa cgatttaggt atcgaatttc caccctactt
    15721 ggcacgccag gcgatatacg ctgtaggatg tatgctagcg gtagcgatgc cattgagggt
    15781 ttctcaaaaa acgtttgtga attttttggc ggcaatagga atgtaatgtt cagctttgcc
    15841 gccattacca cattagggcc tttcctggtt atgtttttta tgcctttccc gttggttttt
    15901 acttactttt tttcggtcat aatggcgcgt ttgtttattg ctgacttatc caaacaaaat
    15961 gtttttaaag ctgttatact ttttccatat caacagattt cattcctgca aatggtgata
    16021 cgctgggaaa aaaatcgtaa acggggaact ctgatatgga agggaagaaa aatataatag
    16081 catctctatg gctaaaggac ttttgctagt tgccagtttt tccattctgt tgctaaatca
    16141 gcaaccgtta atcgcaaata ttaacgatga tttcgaccac cggctctatg tcgcatttat
    16201 ttccggtaac atggccgatt ggggtaaggt gttaagtgaa cttgaggcta tagcttcaaa
    16261 aaatccatcg gcaacaaatc gatttcgact tttacatgca cagtatggct atattggata
    16321 cctgctgggt gtaaacaaaa caagcatagc aaagcaactt attgataaag ctgaaaagca
    16381 ggccaatatt ttagccagca tacctaccta ccaatcaact gccaaagcca tgcaggcagc
    16441 cctgttggct tatcggattt caataagccc ctacaaagcg ccttttcttg ggcctaaaag
    16501 cgcatcgcta atcgacgagg cattggcgtt aaaccccacc aattactttg cgcttatcga
    16561 aaaggggaat gcacgacact acgcaccctc aattgtggga ggcaacccaa ccgaggcggt
    16621 tgagctttac cgtaaagcga ttgagctaat tgaaaaagaa aatccggcga gtaagcccca
    16681 gaaatggtgg tatataaaca catataccca aatgggacta gctgcacaaa aggccggaaa
    16741 taaagattta gctaaaaaaa tctacctaac catactaggc atggagccta actacaagtg
    16801 ggtaagggag gaactgttac caaaagt
//
LOCUS       DAOSAL010000056        16460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_2421_length_16460_cov_49.562024, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000056 DAOSAL010000000
VERSION     DAOSAL010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 16460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16460
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2421_length_16460_cov_49.562024"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="PK049_08285"
     CDS             <1..843
                     /locus_tag="PK049_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOK61595.1"
                     /translation="NLMREVGRLRELTFRSAGGGTGEEVDIDEYDTCENCYKQLIVWD
                     PHEKEILGGYRFHSVDPSKGEEINLSTKHLFHFSNKFIQEYMPQMIELGRSFVQPKYQ
                     SLSLSGKGLYALDNLWDGLGALIVLNPHIRYFFGKVTMYRTYNVEARNILLYFFEKYF
                     RDAENLVTPIHPIEVNIDRQAMAQVFTGKNYEEDYKILSKKIREFGERIPPLINSYMN
                     LSPSMKVFGTVINPYFGEVEETAILITIPDIYQHKVERHISTFIKKLKDKYHLGLRWG
                     KDSK"
     gene            complement(917..>1372)
                     /locus_tag="PK049_08290"
     CDS             complement(917..>1372)
                     /locus_tag="PK049_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOK61596.1"
                     /translation="PARIIVVSSSPQIRYPDCYGIDMAKLSDFVAFRAAIELLKETGQ
                     EGVINSVYRKCKEQQNLPKEQIINYVKEIYRPFTDEQISQKIAQLLTPKDVKAEVKLV
                     YQSIENLHKSCPNDQGDWYFTGDYPTPGGNRVVNNSFINFIEGRNQRAY"
     assembly_gap    1373..1472
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1473..2970)
                     /locus_tag="PK049_08295"
     CDS             complement(<1473..2970)
                     /locus_tag="PK049_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOK61597.1"
                     /translation="MSEQIKHECGIALIRLLKPLEYYQLKYGTWRYGLHKLYLLMEKQ
                     HNRGQDGAGLTTIKFDLAPGKKYIDRERSNSSTPIKDIFDAVNKGINKYSSKPELLND
                     PVFAKENIPFAGELFLGHLRYGTFGNNSIDYVHPVMRDNNWKSRTLVLAGNFNMTNVD
                     ELFKKLVDLGQHPRDYSDTVTVLEKIGHFLDEENQQLFRQYKNDGHPNIEISKLIAEN
                     INVAKILQASTKDFDGGYAMAGLIGHGDAFVVRDPWGIRPAFYYANDEIAVVASERPV
                     IQTVLNISTDDVKEIKPGYALIIKRNGTILHEEIRIPQARKSCSFERIYFSRGTDKDI
                     YKERKRLGEFLTPAILKAINYDHENTVFSYIPNTAETAFLGMVKGIEDYIKIEKKRRI
                     LEKGANLTEKDIDEIISTRLRVEKIAVKDVKLRTFIAQDKGREDLVGHVYDITYGTLR
                     PGVDNLVVIDDSIVRGTTLRESILRILDRLNPARIIVVSSSPQIRYPDC"
     gene            complement(3022..3984)
                     /locus_tag="PK049_08300"
     CDS             complement(3022..3984)
                     /locus_tag="PK049_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOK61598.1"
                     /translation="MGEYINKGDKATRLGFGEGVAEAGGEYPNLVVLGADITSSVGLN
                     LFAERFPERFFSFGIAEQNIAAVAAGLALTNKIPVFSTYGVFAAFRAADQIRISICYN
                     NVKVIIGGAHAGISVGPDGATHQALEDIAVIRSLPNITLISPADANQAKAATLAAIRQ
                     VEGPVYIRFGREPVPNFTEPNQVFEVGKGQVLKAGTDVAIVATGHMVWEALEASKHLA
                     KQGVNAMVVNIHTIKPIDKQLLVDVAKATKRVVTVEEHQVHGGLGSAVAEVLSQEYPV
                     PMKIIGMPDCFGESGTPAELLQKYGLNANSIAQHVFQFVNRFKQ"
     gene            complement(3977..4825)
                     /locus_tag="PK049_08305"
     CDS             complement(3977..4825)
                     /locus_tag="PK049_08305"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOK61599.1"
                     /translation="MATPAIRQLQKTSLKIRQDVIRSLTAAGSGHLGGSLGLADVFTV
                     LYFNTLNHFPKEPNHPNRDRLILSIGHVAPVLYATLANAGYFPHEELLTLRKLGSRLQ
                     GHPGRDHGLPGIELSAGSLGQGLSVAVGMALSAKIDNSKWQVYSVHGDGELQEGSVWE
                     AAMSAAHYKLDNLTAIVDRNFCQIDGYTHKVMELEPLKLKWEAFGWHVFECNGNSIDE
                     LIDVFGKTRNITGKPKVIIAKTQMGFGVNSIQGDYRWHGKAPTTQEAEKFLEELEKYY
                     HELIHG"
     gene            complement(4827..5288)
                     /locus_tag="PK049_08310"
     CDS             complement(4827..5288)
                     /locus_tag="PK049_08310"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOK61600.1"
                     /translation="MRKIFLLLVLLGLSFGIFAQNTNGTTPIPIVNELQAHKAGEGTV
                     TIRQSESITQIHNIRYLHNAKSPGMEGYRIRIFFELGQFARKNSEDIMQAFMEKYPGV
                     PVYQNYQNPYWKVSVGDYRSREEAIKFYNQIIKDYPKAFIIPEHINFPPLN"
     gene            complement(5328..5846)
                     /locus_tag="PK049_08315"
     CDS             complement(5328..5846)
                     /locus_tag="PK049_08315"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HOK61601.1"
                     /translation="MKNFSFILLLSILLFALAGCGKYNDLNIHGVKEYRFRGMKDGVI
                     FLNLTLDVENPNRKSIVIKDIHLKAWLNNRELGKLRSSHKLKIEGKSRKDYEVPLEIV
                     LRTPADAFKLIGEGKNLISVIEVEGYIKGGKFPVVKKINIPRQPLSELANSFQKKFVI
                     TDTLSVQPAVMP"
     gene            complement(5984..6730)
                     /locus_tag="PK049_08320"
     CDS             complement(5984..6730)
                     /locus_tag="PK049_08320"
                     /inference="COORDINATES: protein motif:HMM:NF001030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOK61602.1"
                     /translation="MSGHNKWSTIKRKKGALDAKRSKEFSRIIKEISVAVKEGGADPE
                     GNPRLRLAINNAKGVNMPKENIIRAITKAEKDPENLQELTFEGYGPGGIAIFIECLTD
                     NNNRTISAVRSTLTKKGGNLGTNGSLAFLFSRKGIFTIAKDKINLDEVELDLIDAGAQ
                     NIDDNGDFYVVTTEMEDFGRMNKKLEELKIEVENASLQRIPNDFKSVDKDTAMKFLKL
                     VEELEDNDDVQQVYHNLELTDEVMAAMDNE"
     gene            6881..8278
                     /gene="miaB"
                     /locus_tag="PK049_08325"
     CDS             6881..8278
                     /gene="miaB"
                     /locus_tag="PK049_08325"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010264352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOK61603.1"
                     /translation="MDFNAEDANIFESALSDDSSRPKVYIETYGCQMNVNDSEVVASI
                     LIANGYAITSNINEADVILINTCSIRENAETRVFGRIDLFGQVKKSKPSVLVGVLGCM
                     AERLKDQLLEEKKVVDLVVGPDAYRELPVLLRAAEDGQKGINVLLSREETYADISPVR
                     LDKNGVSAFVSIMRGCNNMCSYCVVPYTRGAERSRDPHTIVREVQELVDAGYREVTLL
                     GQNVNSYHWEEQKGLIKHKVNFSDLLEMVALVDPRLRVRFSTSHPKDLTNEVLYTMAM
                     YENICNHIHLPVQSGSTRILQLMNRKYTREDYLDRIKAIRSIIPDCSISTDIIAGFCT
                     ETEVDHDMTLSIMREVGFDFAYMFKYSERPNTKAARQLKDDVPEEVKTRRLMEIIELQ
                     GKLSEQSKKSDLGKRFEVLIEGESKKSKDEFFGRTSQNKVVVFPKENHKIGEFVFVEI
                     TDCTPATLIGRIVQE"
     gene            complement(8280..9476)
                     /locus_tag="PK049_08330"
     CDS             complement(8280..9476)
                     /locus_tag="PK049_08330"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOK61604.1"
                     /translation="MGSVFETAGRALGEVVYQNRNLDQVISYWIAQNKLWEPFDKYQF
                     AVIVEETTRWWRLLNELHDSEFPKFGSHTHNIVAIYTTLTPFGFPEERKIPKHFKKKL
                     QNKLNQLSNNRAIKESIPDWLDELGFKELGEKWPKEIHSLNHKAPIVLRTNLLKTNVQ
                     ELNNFLKREGYKTKIIPNTHAILLTESANIFKSEAFKLGMFEQQDFSSQQVVPFLNVE
                     PGMRVIDACAGNGGKTLHLASLMNNQGRIIALDTAPWKLEELKRRSRKAGVTNVETKA
                     IETTKTIKRLQDSADRLLIDAPCSGLGVLKRNPDSKWRLTPENIAELRKTQRQILEKY
                     WSMLKVGGCMVYATCSILPSENEEQVKWFLEQMNGRFVLEEEKNLSPSQTGFDGFYMA
                     RLKRIK"
     gene            complement(9532..10260)
                     /locus_tag="PK049_08335"
     CDS             complement(9532..10260)
                     /locus_tag="PK049_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61605.1"
                     /translation="MKKITLSKEEIEDLKDLYQSEIDRAQRRIDSLKSILRKIEKGEL
                     GDEKEVAAPKKRGRKPKVKEATEPTAPKKRGRPKKITAEADKSAQTKEPKKRGRKPKV
                     ASETKTEKPKKGRKKIAKKKVKTSPPKKRGRKPKSLRKGLKPTVGGEKIKWNNLIIDI
                     FKSNDKLLQAKDLTVGALEKLQLPEVEKDRTRMAVATNLTKLTKYEKKILKYTRPDDK
                     IAYYGLAEWFNEDGTLKPEYQNKF"
     gene            complement(11164..12165)
                     /gene="fbp"
                     /locus_tag="PK049_08340"
     CDS             complement(11164..12165)
                     /gene="fbp"
                     /locus_tag="PK049_08340"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HOK61606.1"
                     /translation="MNGYNVKTLTQFIIERQADFPYATGEFTRLLYQVGVAAKLVNKK
                     VNKAGLVDVLGEAGNINVQGEEQKKLDLLANWQFVQALKNSRECCGVASEEDQEIITW
                     DDELSKDGNYIFCMDPLDGSSNIDVNVSIGTVFSIYRRISPRGTKPTMEDFLQPGNKQ
                     VAAGYVIYGSSTMLVYTTGRGVYGFTLDPSIGEFCLSHLNIVTPPDGAIYSINEGNYN
                     HFPEGVKKYIEYCKQEDKPTKRPYSARYIGSLVSDFHRNLLKGGIFMYPPTASAPKGK
                     LRLIYECNPIAFLAEQAGGIATDGKNRILDIQPESLHQRVPLYVGSKNMVQKLQEFL"
     gene            12356..13633
                     /locus_tag="PK049_08345"
     CDS             12356..13633
                     /locus_tag="PK049_08345"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOK61607.1"
                     /translation="MKPIRVFKFGGASVKSAGAIRNLANIVKRFSTENLVIVVSAMAK
                     TTNALEEVLMYWMNADLTAMNTKLKEIKQFHLNEALDLVEGDSNHLLIAELQALFNQL
                     ETQFGQTPTKGYDFHYDQVVSFGELFSTRIVSHYLNFIGIANTLVDARRCLRSDTSYR
                     EGVIDETTSRLLCQREFDFSSANIFLTQGFIAGTEVGTTTTLGREGSDYTAAILANLL
                     DAQDVTIWKDVAGVLSADPKIFSNAVKLSFVSYQEAIELAYCGAQIIHPKTIKPIQNK
                     KIPLYVKSFMEPDAQGTIIAHSDTPVKLPPVLVLKRNQVLITLSPRDFSFVIEDCLSK
                     IFALLYKHRIKVNMVHNSAINFTVCVDNDKLRLGDALEEMKHEFAVRYNSNLELLTIR
                     HYTPEIIENFIGNRVVYLQQRTRSTARFVMDAS"
     gene            complement(13637..14125)
                     /locus_tag="PK049_08350"
     CDS             complement(13637..14125)
                     /locus_tag="PK049_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61608.1"
                     /translation="MNIKFKLILLAIVFESCSNPQPKNVIVDITGKEQDLTLIADSIK
                     YEVVVQASENDIWESERLQGYRNHKSFIDGTFKGILSGKLKVFDYTTNEPLTVEEVRK
                     IIEDHKIDASQIGKLLFTEQWFTDKQGHLHKKILSITFGKSEYSKQGTFKGYSALFTV
                     KY"
     gene            complement(14130..15476)
                     /locus_tag="PK049_08355"
     CDS             complement(14130..15476)
                     /locus_tag="PK049_08355"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOK61609.1"
                     /translation="MGRLAIINATIINEGKTFKGNLIVNGETIEHISTAPSSVDKFLP
                     EEIINAEGLWLLPGVIDDQVHFREPGLTHKGDIYTESKAAVAGGVTSFMEMPNTKPQT
                     TTVELLHDKFKIASQKSLANYSFYLGATNDNIDEITKVDPRNVCGLKVFMGSSTGNML
                     VDNEDALEKIFRFSPTIITTHCEDEDTIRKNLEKYQGLYGNNIDIKYHASIRSAEACY
                     LSSAKAVRLAKRHGARLHILHLSTANELELFNNSIPLAEKKITAEVCVHHLWFNNNDY
                     DRLGWRIKWNPSVKTETDRLALIQGLKNNLLDVVATDHAPHLISEKDNVYTSSASGGP
                     MVQHSLLAMLELSRKGYFTPELVVEKMCHSPATLFRIEKRGFIREGYFADLVLVNPNK
                     SQAVAKENLYYKCGWSPLEGETLSHTVEMTILNGRIVYSKGVFNETIKGKALTFNP"
     gene            complement(15487..16236)
                     /locus_tag="PK049_08360"
     CDS             complement(15487..16236)
                     /locus_tag="PK049_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HOK61610.1"
                     /translation="MSFKEKLVIIPTYNESENISRMIGKVMSLEGNFHLLIVDDGSPD
                     GTASIVKEKQKEYPDRLFMIERSGKLGLGTAYITGFKWALANGYKYIFEMDADFSHNP
                     DDLINLYNACVNGADLAIGSRYVNGVNVVNWPIGRVLMSYFASKYVRMVTGMKVMDTT
                     AGFKCYKAEVLKAINFDKIKHIGYGFQIEMKFTVWKLGFNIVEVPIIFTDRKFGASKM
                     SGGIFNEALWGVVNMKLKSLFVSYKKLHASN"
BASE COUNT         4459 a         3424 c         3372 g         5105 t
ORIGIN      
        1 aacctgatgc gtgaggttgg acggttaagg gaactcacat tcagatcggc aggtggtgga
       61 accggtgagg aggtagatat cgatgagtac gacacctgcg aaaattgtta caagcagctt
      121 attgtatggg atccccatga aaaggagata cttggtggct accgctttca tagcgttgat
      181 ccctctaaag gcgaggagat aaatctatct accaaacatt tgtttcactt ttccaataag
      241 tttattcagg aatatatgcc tcaaatgatt gagttgggtc gatcatttgt tcagcctaaa
      301 tatcaatcac tttcccttag cggaaaggga ttgtatgcgc tcgataacct ttgggatggc
      361 ctgggagcac tcattgtgct aaatcctcat atcaggtatt tcttcggcaa ggttaccatg
      421 tatcgcacgt acaatgttga agcacgtaat attttgcttt acttttttga aaagtatttc
      481 agggatgctg agaacctggt aacaccaatt catccaattg aggtaaatat cgataggcag
      541 gctatggccc aggtttttac tggtaagaat tatgaagaag actacaagat tctgtccaaa
      601 aaaattcgcg agtttggcga gcgtattcca ccgcttatta actcttacat gaacttaagc
      661 ccatccatga aagtgtttgg aactgttatt aatccttact ttggcgaagt tgaagaaaca
      721 gctattctca taaccattcc tgatatttac cagcataagg ttgagcggca tatatcaacc
      781 tttataaaga agttaaagga taagtaccac ttgggtttaa ggtgggggaa ggattcaaaa
      841 taaccctgaa agttaaacct ctactattta tccttttttt tatcctttaa cctttatcct
      901 ttaaccttta tccttgtcaa tacgcccttt gatttcggcc ctcaataaag ttgataaacg
      961 aattgtttac cactcggttt ccaccggggg tggggtagtc gcctgtaaag taccagtcgc
     1021 cttggtcgtt agggcaagac ttgtgtagat tttcaatgga ttggtaaact agttttactt
     1081 ctgcctttac atcctttggg gtaagaagct gagctatttt ttgcgaaatc tgctcatcgg
     1141 taaacgggcg gtagatttct tttacatagt taattatttg ctctttgggc aggttttgtt
     1201 gctctttgca cttgcggtaa acgctattaa taacaccctc ttgtccggtt tccttgagca
     1261 gttcaatggc tgcacggaag gccacaaaat cgctcagttt ggccatatca attccgtagc
     1321 aatcggggta gcgaatttgt ggcgatgatg atacaacaat tatgcgggca ggnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnagcaatcg gggtagcgaa tttgtggcga
     1501 tgatgataca acaattatgc gggcagggtt aagcctatca agtattcgga gaatgctttc
     1561 ccgaagggtt gtccctcgaa caattgaatc gtcaattacc accaggttat caactccagg
     1621 gcgaagagta ccataggtga tgtcgtaaac atgcccaacc aaatcttcgc gacctttatc
     1681 ctgtgcaata aatgtgcgta gcttaacgtc ttttacggca attttttcta cccttaggcg
     1741 agtggatatt atttcatcga tatccttttc ggtaaggtta gccccttttt ccagaattct
     1801 gcgtttcttt tcaattttta tgtagtcctc tatgcctttt accatgccaa gaaaagctgt
     1861 ttcggcagta ttgggaatgt atgaaaaaac agtattctca tggtcgtagt ttatggcttt
     1921 cagtatggca ggggttaaaa attcgcctag ccgtttacgt tccttgtata tgtccttatc
     1981 ggttccccgt gagaagtaga tccgttcaaa cgagcaggat tttcgtgctt ggggtattct
     2041 tatttcctcg tgtagtattg taccattgcg cttaattata agcgcatagc cgggcttaat
     2101 ttcttttaca tcgtcggttg aaatgttaag cacagtctga ataaccgggc gttccgatgc
     2161 aacaacagct atctcatcgt tggcatagta gaaagccgga cgaattcccc atgggtcacg
     2221 aaccacaaat gcatcgccat ggcctataag gccagccata gcataaccac cgtcaaaatc
     2281 tttagtggat gcctgaagga tttttgcaac atttatgttt tctgcaatta gcttgcttat
     2341 ctcaatgttg ggatgaccat cgtttttgta ctgccggaaa agctgctggt tttcctcgtc
     2401 gaggaaatgt ccaatttttt caagtacggt aaccgtatcg gagtagtcgc ggggatgctg
     2461 gcctaaatcg acgagctttt tgaaaagctc atccacgttt gtcatgttga agttaccagc
     2521 taggacaagc gtacgcgatt tccagttgtt gtcgcgcatt accggatgaa cataatcaat
     2581 ggagttatta ccaaaggttc cgtagcgcag gtgtcccagg aatagttccc cggcaaacgg
     2641 aatattttcc ttggcaaaaa ccggatcgtt tagcagttcg ggtttcgatg agtacttatt
     2701 gatccctttg ttaacagcat caaaaatatc cttgatggga gttgaagagt ttgagcgttc
     2761 cctatcgata tactttttgc ccggggcaag gtcgaactta atggtggtta accctgcacc
     2821 gtcctgccca cggttatgtt gcttttccat aagcaggtaa agcttgtgaa gtccgtatcg
     2881 ccaggtacca tacttaagct ggtagtactc caatggttta agcaacctaa tcagtgcaat
     2941 cccgcattcg tgtttaatct gctcgctcat atcttcctaa attattcaat taaaaaaggt
     3001 agatagaatc tacctgaagt tttattgttt aaatcgattt acaaactgaa aaacatgctg
     3061 ggcaattgaa ttggcattaa gaccgtactt ctgcaacaac tcagcaggtg ttcctgattc
     3121 gccaaaacaa tcgggcatgc caattatctt catggggaca gggtattcct gcgaaagcac
     3181 ttcagccact gcgctgccaa gtcctccatg aacctgatgc tcctcaactg ttacaacacg
     3241 ctttgttgct ttagcaacat caaccaatag ctgcttatca ataggtttga tggtatgaat
     3301 gtttaccacc atggcattaa caccctgctt ggcaagatgc tttgatgcct cgagtgcctc
     3361 ccacaccatg tgaccggtgg ctactattgc aacatcggtt cctgctttta ggacttgtcc
     3421 cttaccaacc tcaaacacct gattgggctc ggtaaaattg ggcactggtt cgcgtccgaa
     3481 acggatgtaa accggccctt caacctgcct gatggcggca agggtggcag cctttgcctg
     3541 gttggcatcg gctggtgaaa ttagtgtgat atttgggagc gaacggataa ctgcaatatc
     3601 ctcaagggct tggtgtgttg caccatcggg gccaaccgat attccggcat gggcgccacc
     3661 aattattact ttaacgttat tgtagcaaat tgaaattctt atttgatcgg cagcacggaa
     3721 tgctgcaaaa acgccgtatg tagaaaagac aggaatctta ttggttaatg ctaacccagc
     3781 cgcaacagcg gctatatttt gttcagcgat accaaaggag aagaacctct cggggaaacg
     3841 ttcggcaaaa agattcaatc caaccgagga ggtgatatcg gccccaagca caaccaggtt
     3901 ggggtattcc ccaccggctt cggcaactcc ttcaccaaat cccaggcggg tagctttatc
     3961 acctttattt atatactcac ccatggatta gttcgtggta atatttttct aattcttcca
     4021 agaatttctc tgcctcctgc gtggttgggg ctttaccatg ccaacggtaa tcgccttgaa
     4081 tggaattaac accaaagccc atttgggttt tagcaataat cactttaggt ttgccagtga
     4141 tgttgcgggt tttaccaaaa acatcaatta gctcatcaat actattgcca ttgcattcaa
     4201 atacgtgcca cccaaatgcc tcccatttaa gtttaagggg ctcaagttcc attactttat
     4261 gggtgtagcc atcaatctga cagaaatttc gatcaacaat ggcagtcagg ttatcgagtt
     4321 tgtagtgtgc ggcgctcatg gcagcctccc aaacggatcc ttcctgtagt tcgccatcgc
     4381 catgcacaga gtatacttgc catttggaat tatcaatttt agcgctgagc gccataccaa
     4441 cagcaaccga taacccctga cctaacgaac cggctgagag ttcaattcca ggtaaaccgt
     4501 gatcgcgtcc cggatggcct tgtagccgtg aacctagctt gcgtaaggta agtagttcct
     4561 catgcgggaa gtatcctgca ttggcaagcg ttgcatacaa aactggtgca acatgaccaa
     4621 tggaaaggat tagcctatcg cggttagggt ggtttggttc tttagggaaa tggtttaggg
     4681 tgttaaagta tagaacggtg aaaacatcgg caagtccaag tgagcctccc aggtgtcccg
     4741 atccggcggc cgtcaggctt cgaattacat cctggcggat ctttagcgaa gtcttttgca
     4801 gttgccggat tgctggtgtt gccatactaa tttagaggtg gaaaatttat atgttcggga
     4861 ataatgaaag ctttgggata atccttgata atctggttgt aaaacttgat agcctcttcg
     4921 cgcgagcggt aatcgcccac tgataccttc cagtatgggt tttggtaatt ctggtacaca
     4981 ggcactccag gatatttttc cataaaggcc tgcattatat cttcagaatt tttccgggca
     5041 aactgtccga gctcaaagaa tattcgaatc ctgtaccctt ccatccccgg cgatttggca
     5101 ttatgcaagt agcgaatgtt gtggatttga gtaattgatt cgctctgcct gatagttact
     5161 gtaccttctc cagccttgtg tgcttgaagc tcattaacta ttggtatggg cgtggtaccg
     5221 tttgtgtttt gggcaaaaat gccaaaactt aaaccgagta acactaggag taggaaaatt
     5281 ttcctcataa agagtataat ttcgttgcaa aaatagcaaa aatttatcta tggcattaca
     5341 gcaggttgaa ccgaaagagt atcggtaata acaaatttct tttggaatga gttggctagc
     5401 tcgcttagtg gctgccgggg gatgttaatt ttttttacaa ccgggaattt tcccccttta
     5461 atgtagcctt caacctctat tacgctaatc aggtttttcc cttcgcctat aagtttaaat
     5521 gcatcggctg gggtacggag cacaatctct aatgggactt cataatcttt tcgacttttg
     5581 ccctctattt tcaatttatg gctactcctt aactttccaa gttccctatt gtttagccaa
     5641 gcttttaggt gaatatcttt tattacaatt gatttccggt tagggttttc aacatcgagc
     5701 gttaggttta ggaatattac accatccttc atcccccgaa atctatactc cttaacacca
     5761 tgaatattta aatcgttgta tttaccacag cctgctaaag caaatagtaa aatggaaaga
     5821 agtaaaataa aagaaaaatt cttcatgctt tatgttttaa ttagtaagag caaatgttat
     5881 accataccca caaaagtaat atgattgatt ggccacggta tacagtgtat actaaaaata
     5941 aaaaaaactc ccctgcattg gggagtaaaa ggttttattg tgtttactcg ttatccatgg
     6001 cagccataac ctcatcggta agctccaggt tatggtaaac ctgctgaaca tcgtcattat
     6061 cctctagttc ctcaaccaac ttcaggaact tcattgcagt atccttatca accgatttga
     6121 aatcgttagg aatacgttgc aggctggcat tttccacctc tattttcagt tcctcaagct
     6181 ttttattcat tctgccaaaa tcttccattt cggtagtaac cacatagaaa tcaccattgt
     6241 catcgatgtt ttgggccccg gcatcaataa gatcgagttc cacctcatcg aggttgattt
     6301 tatcctttgc aatggtaaag atgccttttc ggctaaaaag gaatgccagc gaaccgttgg
     6361 ttccaaggtt tccacctttt ttggtcaggg ttgaacggac tgcgctaatg gttcggttgt
     6421 tattatcggt aaggcactca ataaagatgg caatgccccc cgggccatag ccctcaaagg
     6481 taagttcctg aaggttttca ggatcttttt cagccttagt aatagcccgt atgatattct
     6541 ctttgggcat gtttacccct ttggcgttat taatggcaag tcttaatcgt gggttgccct
     6601 cagggtcagc ccctccctcc tttacagcaa cggatatttc tttaataatt cttgaaaatt
     6661 cctttgaacg cttggcatca agtgcaccct tcttgcgttt aatggttgac catttattgt
     6721 gtccactcat agttttttat ttttgtctca ggtaaagtgc aaagttagct acaaacttgc
     6781 gctttacaaa aatatttaac ctctattgat ttgtatcact ttatacactt aaagcaaaat
     6841 attcataaat tcttaccttt gcaaaaaaat aagatttcca atggatttta atgccgaaga
     6901 tgccaacatt tttgaaagtg cgctgagcga tgatagtagc cgccctaagg tttacattga
     6961 aacctatggc tgccagatga atgtgaacga tagcgaggtt gtggcatcaa ttctaatagc
     7021 caatggatac gcaataacta gcaacattaa cgaagccgat gttatcctga taaacacctg
     7081 ctccattcgt gagaatgctg aaacacgtgt gtttggacga attgatttat ttgggcaagt
     7141 aaagaagtca aagccatcgg tattggttgg ggtattgggc tgtatggctg aacggctaaa
     7201 ggatcagctt ttagaagaaa agaaagtagt tgatttggtg gttggccccg atgcttaccg
     7261 tgaactaccg gttctgctgc gtgctgccga ggatggtcaa aagggcatca atgttttact
     7321 ctcccgggaa gaaacctatg ccgatattag ccctgttcgg ctcgataaaa atggagtttc
     7381 agcctttgtt tcaatcatgc gcggatgcaa caacatgtgt tcttactgtg ttgtaccata
     7441 cacacgtgga gcagagcgaa gccgcgatcc acataccatt gtaagggagg ttcaggaact
     7501 agttgatgct ggttaccgcg aggttactct gcttggccaa aatgtaaact cctaccactg
     7561 ggaggagcaa aaaggcctaa tcaagcacaa ggtaaacttt tccgatttgc tcgaaatggt
     7621 tgcccttgtc gacccacggt taagggttcg cttttccacc tcacacccca aggatttaac
     7681 caatgaggtg ctttacacca tggccatgta cgagaacatt tgcaaccata ttcatctccc
     7741 cgttcaatct ggctccaccc gcattcttca gctaatgaac cgcaagtaca cccgggagga
     7801 ttacctcgac agaataaaag ctatacgctc cattatcccc gattgctcta tatcaaccga
     7861 tattatagct ggtttctgca cggaaaccga ggtcgatcac gatatgaccc tcagcattat
     7921 gcgtgaagtg ggctttgact ttgcatacat gttcaagtat tccgagagac ctaacacaaa
     7981 ggctgctcgc cagctaaagg acgatgtgcc tgaggaggtt aaaactcgca ggttaatgga
     8041 aatcattgaa ttacaaggca aactttccga acaaagcaag aagagcgatt taggcaaacg
     8101 ctttgaagtg cttattgaag gagaatccaa aaagtcaaaa gatgaatttt ttggccgaac
     8161 cagccagaat aaggttgttg tattcccaaa ggagaaccac aaaattggag agtttgtatt
     8221 tgtagagata actgactgca ctccggcaac attaattggt cgcattgtgc aggagtagac
     8281 tatttaatac gctttagcct tgccatgtaa aacccatcaa accctgtttg ggatggggat
     8341 aggttttttt cctcttccag tacaaagcgg ccattcattt gctccaggaa ccatttaacc
     8401 tgctcttcat tctcagaggg tagaatacta caggtagcat aaaccataca gcccccaacc
     8461 ttaagcattg accaatactt ttcaagtatt tgtctttgag tttttcgtaa ctcagcaatg
     8521 ttttcaggag tcaaccgcca tttggagtca gggttacgct taagcactcc caagcccgag
     8581 catggggcgt caatcagcaa cctatcggca ctatcctgta aacgttttat cgtttttgtt
     8641 gtttctatgg ctttggtctc aacatttgta accccagcct ttcttgacct ccttttaagc
     8701 tcttcaagtt tccatggggc agtatcaagg gcaataattc ttccctgatt attcatgagg
     8761 cttgccaggt gcaatgtttt tccaccattc ccggcacaag catcaataac acgcatgccc
     8821 ggttccacat ttaaaaatgg aactacttgc tgcgatgaaa aatcctgctg ctcaaacatt
     8881 ccaagcttaa atgcctcgga tttaaaaatg ttggccgatt cggttagcag tatggcgtgg
     8941 gtgttgggaa taatttttgt tttatacccc tcacgtttta aaaaattatt aagctcttga
     9001 acgttcgttt ttaaaaggtt tgttcttagg actattgggg ctttatgatt taagctatga
     9061 atctcctttg gccatttttc gcccagttcc ttaaaaccta gttcatcgag ccaatcggga
     9121 atagactcct ttattgccct attatttgaa agctgattta gtttgttctg aagcttcttt
     9181 ttaaaatgtt ttggaatctt acgctcctct ggaaatccaa agggtgttag ggttgtataa
     9241 atggctacaa tgttatgagt gtgtgatccg aattttggaa attcactatc gtgcagctcg
     9301 ttcagcaacc tccaccatcg tgttgtttcc tccacaataa ctgcaaactg gtacttatca
     9361 aagggttccc ataatttatt ttgagcaatc cagtacgata ttacctggtc taaatttcga
     9421 ttttggtaaa caacctcacc aagggcgcga cctgccgttt caaatacact tcccataata
     9481 taaaaattaa aggccgcaat ttacggcctt tttatcgata aagaatgaaa tttagaattt
     9541 attttggtac tcaggcttta gggttccatc ctcattgaac cattctgcca aaccgtagta
     9601 ggctatttta tcgtccggac gggtatactt caaaatcttt ttttcgtatt ttgttagttt
     9661 tgtaagattg gttgctacgg ccatacgggt tctgtccttt tcaacctcgg gcagctgaag
     9721 tttttcaaga gctcccactg taaggtcctt tgcctggagc aatttatcgt tcgatttaaa
     9781 aatatcgata attaaattgt tccatttaat tttttcgcca ccaacggttg gttttaaacc
     9841 ctttcgtaac gattttggtt tccgtcccct tttcttagga ggcgatgttt taactttttt
     9901 cttggctatt tttttacgac ctttttttgg cttttcagtt tttgtttctg aggctacttt
     9961 tggtttcctt cctcttttct tgggctcttt tgtttgggca cttttatcgg cctcggctgt
    10021 aattttcttg ggacgtcccc tttttttggg ggcagtgggt tctgtggctt cttttacttt
    10081 tggtttgcga cctctttttt taggagcggc aacttccttt tcatcgccca attcgccctt
    10141 ttcaattttt cgcaaaattg attttaaact atcaattcgt cgttgtgccc tatcaatctc
    10201 tgattggtaa agatctttta gatcttcaat ctcctctttt gagagtgtga tttttttcat
    10261 ggttggttag tttagtgttt tactcctgat ttctaattat tttgaattgc aatttaaaaa
    10321 cattttattt taaaatgcaa atatttacaa aaaattgatt aaaaaatttt atttaattat
    10381 tttttcaatg tccataaaaa ccagaaaatg aaatatatag gataagtcaa ccttcaattg
    10441 agcaaattat tatttgaaaa taaaatttag ttggatttaa ctactttccc ttccttatta
    10501 aagtactgct aatgttatat acgaccaata ttaataccaa ataccatgaa ttaacaaatt
    10561 caaatgggct aattttagag gaatgagtcg gaatgaacct gaatgattcg gaatgattcg
    10621 gaataagctg aatatgatgg agttagagga agttagagga atagtaaaaa gtgaacggct
    10681 aaaccaaatg accatgaatt aacaaattca accgggctaa ttttgaggaa tgagtcggaa
    10741 taaacctgaa tgattcggaa tgattcggaa taatttgaat atgatggagt tagaggaagt
    10801 tagaggaata gtaaaaagtg aacggctaaa ccaaatgacc atgaattaac aaattcaacc
    10861 gggctaataa aaaatgtgga tacagaaaca tttttagagg aagttggtta tatgaatttt
    10921 caattgacga atcgactaac aaaatttaat cattccgaaa tcccggaatt aaaaattttg
    10981 ataagtctgg attacttcga taactcaata gaaacaagct gtttaaatgt tttatggtgt
    11041 ttctattgac taaactcgga taatataatc cataaagtag ttaatggtta atgtagagga
    11101 attgataaaa tatcatgttt acgcaccgtt tagcgcgaat aatatattta tttattgagg
    11161 tttctataaa aattcctgaa gcttttgaac catatttttt gaacctacat aaagaggaac
    11221 tctttggtga agcgattctg gctgaatgtc gagaattcta tttttaccat cggtggcaat
    11281 gcctccggct tgttcggcca ggaaagcaat agggttgcac tcgtaaatta agcgcaactt
    11341 gccttttggt gccgatgcgg ttggcggata catgaaaata cctcccttaa ggagattcct
    11401 gtgaaagtca gaaaccaacg aaccgatata ccttgcagag taaggtcttt ttgtgggctt
    11461 atcttcctgc ttacaatact caatgtattt tttaacaccc tcagggaagt ggttatagtt
    11521 accttcgttt atggagtata ttgccccatc ggggggcgta actatattaa ggtgcgatag
    11581 gcaaaactca cctatggagg ggtcaagggt gaaaccgtaa actccacggc ctgtggtata
    11641 aacaagcata gttgacgatc cgtatattac gtaccctgcg gctacctgtt tattgccagg
    11701 ctgcaggaaa tcctccatgg taggttttgt tccacggggg cttattcgtc ggtagatact
    11761 aaaaacagta cctatcgata catttacatc aatattcgat gatccatcga gcggatccat
    11821 acaaaagata taattgccat ccttgctaag ttcatcgtcc caggtaatta tttcttgatc
    11881 ttcttccgat gctacaccgc agcactccct gctgtttttt agcgcttgta caaattgcca
    11941 gttggctaac aggtcgagtt ttttctgttc ctcgccttgc acgtttatgt tacctgcctc
    12001 gcccagaacg tcaacaagtc cagctttgtt aaccttcttg tttacaagtt ttgctgcaac
    12061 tcccacttgg taaagcaatc gggtgaattc ccctgtagca taggggaaat cggcttgcct
    12121 ctcaatgata aactgtgtta gtgtttttac attgtatccg ttcatgatag ttattaattc
    12181 taatttgtga gtaaaattag ccattttttc cgttgcaaag gcatggcatg acaaaaaaaa
    12241 atcaaattta aaacgtttgc gatgctattc cttaaccagc aatcgctaaa aaaagtaact
    12301 cgcatgttaa gagtatatac ttagcttttt tgtaagtttg agaaattttt tggcaatgaa
    12361 acccattcgt gtctttaagt ttggcggagc ctcggttaag tcggccggtg ctattagaaa
    12421 tttggccaat attgtaaaac gattttccac tgaaaacttg gtgattgttg tttcggctat
    12481 ggctaaaaca accaatgctc tggaggaggt tttaatgtac tggatgaatg ccgatttaac
    12541 ggcaatgaat actaaactca aggagattaa gcaattccac ctaaacgagg cactggattt
    12601 ggttgagggg gattccaacc acttgcttat tgctgagctg caggctctgt tcaaccagtt
    12661 agaaacccag tttggccaga ctcctacaaa gggatacgat ttccattacg atcaggttgt
    12721 gtcgttcggc gagctcttct ctacccggat tgtcagccac taccttaatt ttattggcat
    12781 tgccaatact ttagtggatg caaggcgatg ccttaggtcc gatacatctt accgtgaagg
    12841 agtaattgat gaaacgacat cgcggctatt gtgccagcgt gagttcgatt tctcatcggc
    12901 aaatatcttt ttaacccaag gctttatagc aggaaccgag gtgggaacaa caaccacgct
    12961 agggcgtgag ggttccgatt acacggctgc cattcttgct aatcttctcg atgctcagga
    13021 cgtaaccatt tggaaagatg ttgcaggtgt gctaagcgcc gatcctaaga ttttcagcaa
    13081 tgcggttaag cttagctttg tgtcgtatca agaagctatt gagcttgcct attgcggagc
    13141 acaaattatt cacccaaaaa caataaaacc catacagaat aaaaagatac ccctttatgt
    13201 aaaatcgttt atggagcccg atgctcaggg aactatcatt gcccattccg atactcccgt
    13261 taagctaccc cctgtgttgg tactcaaaag gaatcaggtt ctaataaccc tttcgccccg
    13321 cgatttttcg tttgtaattg aggattgcct cagtaagata tttgcgttgc tatataaaca
    13381 tcgcattaag gttaatatgg tgcataactc agccataaac tttaccgttt gcgttgacaa
    13441 cgataagctc cggcttggtg atgcactgga agaaatgaaa cacgaatttg ccgttcgtta
    13501 caacagtaac cttgaactgt taaccattcg gcattacacg ccggaaataa ttgagaattt
    13561 tattggcaat cgggttgttt atctgcaaca aagaaccaga agcactgcac gctttgttat
    13621 ggacgccagc tagcgcctag tattttaccg tgaatagcgc gctataacct ttaaaggttc
    13681 cttgtttgct atactcggat ttaccaaagg ttatggagag tatcttttta tggaggtgcc
    13741 cttgtttatc agtaaaccac tgttccgtaa aaagtagttt gccaatttgt gaagcatcta
    13801 ttttatggtc ttcaattatc tttctaacct cttcaacggt caatggttcg ttggttgtat
    13861 agtcaaaaac ctttaattta ccgctaagta tacctttaaa tgtgccatca ataaaacttt
    13921 tgtggttccg gtagccttga agcctttcac tttcccaaat atcattttcg gatgcttgaa
    13981 ctacaacctc atacttaatg ctatcggcaa ttaaggttaa gtcctgctcc tttcctgtaa
    14041 tgtcaacaat tacattttta ggctgcggat tgctgcacga ctcaaacaca atagcaagca
    14101 aaatcaactt aaacttaatg ttcattggtt taggggttaa aggttagagc ttttccctta
    14161 atggtttcat tgaaaactcc tttactgtaa acaattctcc cgtttaaaat tgtcatttca
    14221 accgtgtgtg aaagcgtttc tccctccagg ggcgaccagc cacacttata gtacaggttc
    14281 tccttggcaa cagcctgtga tttatttgga ttaacaagca caaggtcggc aaagtatccc
    14341 tcgcgtataa agcctcgttt ttcaatcctg aaaagagtgg ctggggagtg acacatcttt
    14401 tcaactacaa gctcaggcgt aaaataccct ttcctactta gctcaagcat agctaacagc
    14461 gagtgctgaa ccatagggcc tcccgatgct gaactagtgt acacattatc tttttcacta
    14521 atcaggtgtg gggcatgatc ggtggccact acatcgagca ggttgttttt taaaccctgg
    14581 ataagggcta accggtcggt ttcagttttt acgcttgggt tccacttaat acgccagccc
    14641 aacctgtcgt agtcattatt attaaaccat aaatggtgaa cgcaaacctc tgcggtaatc
    14701 tttttctcgg ccaagggtat gctgttgttg aaaagttcaa gctcgttggc tgttgaaaga
    14761 tgaaggatat gtaggcgagc gccatgtctt tttgccagtc taactgcttt tgccgatgat
    14821 aggtagcaag cctcagcgct ccttatgctt gcgtgatatt ttatatcgat gttgtttccg
    14881 tataaccctt ggtatttttc aaggtttttg cggatagtat cctcgtcttc gcagtgtgtg
    14941 gtaattatag taggtgagaa cctaaagatt ttttcaagcg catcctcatt atcaaccagc
    15001 atattaccgg ttgatgaacc cataaaaacc tttaatccgc aaacatttct agggtcaacc
    15061 tttgtaatct catcaatatt atcgttggtg gcgcctaaat agaacgagta attggctaac
    15121 gatttttggc tagcaatctt aaatttatcg tggagtagtt caacggtagt agtttgtggt
    15181 ttagtattag gcatctccat aaatgatgta acaccaccag caacagccgc tttcgattcg
    15241 gtgtaaatat cacccttatg ggtaaggcct ggctcacgga agtgcacctg atcgtcaata
    15301 acgccaggta aaagccataa gccctcagca tttattatct cctcgggcaa gaacttatct
    15361 acacttgatg gggcggtact aatatgctca atggtttcac cattaacaat taggtttccc
    15421 ttaaaggttt tgccctcgtt aataatggta gcatttataa tggctagtcg gcccatttat
    15481 ggttatttag tttgatgcat gaagtttttt gtacgaaaca aaaaggcttt taagtttcat
    15541 gttaaccacg ccccataatg cctcattgaa aatacctcca ctcatttttg aagcaccaaa
    15601 ttttctgtcg gtaaatatta taggtacttc aactatgttg aagccaagtt tccacacggt
    15661 gaatttcatt tctatctgaa aaccgtaacc tatatgcttt atcttgtcaa aattgatggc
    15721 ctttagcact tcggctttat aacatttaaa acctgcagta gtatccatca ctttcattcc
    15781 tgttaccatc cgtacatact tagaggcaaa gtacgacata agcacacgtc caatgggcca
    15841 gttcacaaca tttaccccgt taacatacct cgagccaata gccagatcgg ctccgttaac
    15901 gcaggcattg tacaggttaa ttaaatcgtc ggggttgtgg gaaaaatccg catccatctc
    15961 aaaaatatac ttatagccat tggctagagc ccatttaaat ccagtgatat atgctgtccc
    16021 caatcccagt ttccctgagc gttcaatcat aaaaagccta tcggggtatt ccttttgttt
    16081 ttccttaact atactggctg tgccatcggg tgagccatcg tcaacaataa gaagatggaa
    16141 gttcccttca agcgacatta ccttgccaat cattcgggaa atgttctcac tttcgttgta
    16201 ggtagggata attacgagtt tctctttaaa cgacatctga atatgattgt agtttgtaat
    16261 actataattt ataaactatt gtgcgaagat agcgtttttt acccaaaaac taactatctc
    16321 aagcatgaaa gcaaaatttt tggtttgata atcaggggat aggctttcat gaaacactct
    16381 ttaacaaaaa tattctgtat agacccttgc gtaattaaaa acgcttttct acttttgcac
    16441 ccgctttcgg aggggagcgc
//
LOCUS       DAOSAL010000057        16402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_2436_length_16402_cov_46.241512, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000057 DAOSAL010000000
VERSION     DAOSAL010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 16402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16402
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2436_length_16402_cov_46.241512"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="PK049_08365"
     CDS             <1..492
                     /locus_tag="PK049_08365"
                     /inference="COORDINATES: protein motif:HMM:NF028012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5063 domain-containing protein"
                     /protein_id="HOK61611.1"
                     /translation="LPLLYYKATLLPETEPVYDENNEQFVTESDYSSVENKVEYLLGQ
                     HNQYVEVNDPRVDELTGLYTASIAEYMADIYQDLKNFVLRYQVGNTYVMNDALWECIN
                     NFKDFWGIRLANLIRAFHILAHQNIDLDNIKHPDESEGSERDTSDWFVTRRQQDMDHN
                     ELM"
     gene            492..1082
                     /locus_tag="PK049_08370"
     CDS             492..1082
                     /locus_tag="PK049_08370"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOK61612.1"
                     /translation="MFASSIDKETLNSLPRIHFTGQIVVAEKESDLLQWLPMLCQEKI
                     IGFDTESKPSFKKGKINGISLLQLANSKLAILVRLKKTGIPDTLKEFLENPEIVKVGA
                     AIHDDLKGLLKLYRFTPNGFIDLQRIAPTYGIKELSVKKLSAVVLGATVSKGQQLSNW
                     EADVLTEAQKIYASTDAWVCREIYLKLISHTPKQEH"
     gene            1082..2269
                     /locus_tag="PK049_08375"
     CDS             1082..2269
                     /locus_tag="PK049_08375"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HOK61613.1"
                     /translation="MERPRITLKPGKEQSLLRFHPWIFSGAIKSVTEMPNEGQVVDVL
                     GSDGKFLATGHFASGSIAVRVLSFQPVEVDRTFWLSRISNALNLRRAIGLYPNTDTNI
                     FRLVHGESDLLPGLIVDIYGDTAVMQCHSVGMYLARTEIANEIMQVLPNTVTAVYDKS
                     SGTLPYREKYNPSDGYISGADNARVPYMEYGNKYHISWTDGQKTGFFIDQRENRLLLQ
                     YYAKGKSVLNTFGYTGGFSVSALRGGATEVITVDSSAQAITLANQNIELNFGANAPHA
                     GIAMDTFEYLRTCNRTFDIIALDPPAFAKHNDALRNALQAYKRLNLAAIRRLNPGGLL
                     FTFSCSQVVNKTDFRNAVFSAAAISGRQVSIIHQLTQPADHPINIYHPEGEYLKGLVL
                     SVI"
     gene            complement(2274..3047)
                     /gene="yaaA"
                     /locus_tag="PK049_08380"
     CDS             complement(2274..3047)
                     /gene="yaaA"
                     /locus_tag="PK049_08380"
                     /inference="COORDINATES: protein motif:HMM:NF002542.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HOK61614.1"
                     /translation="MGNYTNYCIVLSPAKTLRSNLKVAGKNYSEPVFLDRAQELVKQL
                     KTYSPVELKDILGVSQDIADLNFTRYANWHLPFTPENSTPAILTFAGDLYEGLQATDF
                     TDDELAFSNRIIFMLSGLYGVLRAFDLMQPYRLEMGSKIQVGQHKSLYDFWGDSITGL
                     LDQNVSDGYLVNLASKEYSRAINDKHFGDRFITIEFKENRPEGLKVIPILSKRARGLM
                     ARFAVKNKIFNVEDLKLFDYEGYSYSDLLSTATKWVFVR"
     gene            3137..>4452
                     /locus_tag="PK049_08385"
     CDS             3137..>4452
                     /locus_tag="PK049_08385"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOK61615.1"
                     /translation="MSNNSLPVFKISKNVCHNWWTAIFIVISFIALALPLQSFSRPGT
                     KVVLQLKWKHQFQFAGYYAAIEKGFYAQEDLDVELKELPQNTNVITEVLEGRAQFGIA
                     NADIIIHYVNGAPVVVLAPIFQSSPAALVSRAERNITRPQDIAGKTIEINAKKSGSVE
                     VLTMLSLEGVKPSQYQVTESSLSLNKLLNNQTDASEVYLTNEPYFLEKFGIPYNLILP
                     HKYGVDFYAECLFTTKEMISKNPELVDAFLRASIKGWEYALENSEEIAQVIQSKYKTT
                     KTLDHLIFESQKVKEFIQPNFINVGHSNKGRWLQMMETLLQAGLINKTKPIDALLYNP
                     QQKKHSPFTDKWFILVIGTLLLLTFILHFWYISRVNKRKQQLVNTHKQQVESLKQELQ
                     MLNTHLEETVQRNKDLESFKESLLSNFSFEIRTPLNNIIGYSELLN"
     assembly_gap    4453..4552
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4553..5343
                     /locus_tag="PK049_08390"
     CDS             <4553..5343
                     /locus_tag="PK049_08390"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOK61616.1"
                     /translation="SDRNLYKSIFQRLINNAVKFTSKGYIEIGVRKSEKNGHLLFWIQ
                     DSGTGIEIEKSKTLFNRLNIEESRDGFGVLQMGLPVVKGIVDLLNGKIWVETAEGTGT
                     TVNVEVPYTPIGSHKAKTYHKKSPFYIHQDPPKLKNKKILIVEDIQSNYILLSRLLEE
                     TGCKLEHAKTGDEALKKVENTPDFDLIFMDLRLADMDGIEITRQIRKKNTRVVIVAQT
                     AYSSGVKVNMSIEAGCNDFITKPISRIDLYNVLRKYLVTDDHTA"
     gene            complement(5340..6359)
                     /gene="tsaD"
                     /locus_tag="PK049_08395"
     CDS             complement(5340..6359)
                     /gene="tsaD"
                     /locus_tag="PK049_08395"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005865403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOK61617.1"
                     /translation="MSVTILGIESSCDDTSAAVIRDQLLLSNVISSQDVHKQYGGVVP
                     ELASRAHQANIIPVVDTALRYAGVTIAEIDAIAYTRGPGLLGSLLVGASFAKGAALTL
                     GVPIISVNHLQGHVLSHFIKDEPNAIHPSFPFLCLLVSGGHTQIMLVRGPMDMEIVGQ
                     TIDDAAGEAFDKCAKVLGLPYPGGPVIDKLAIEGNPGFIKFAKPNIPDLDFSFSGLKT
                     SFLYTLRDKLNTEPNFVNEHLPDICASLQQTIIEILLSKVEQAVRKYRIKEIAIAGGV
                     AANSGLRTALQLLGQKRGWKVYLPKKQFTTDNAAMIAIVGYYSYLAGKTSPLDEPPLA
                     RLPLG"
     gene            6706..11022
                     /locus_tag="PK049_08400"
     CDS             6706..11022
                     /locus_tag="PK049_08400"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOK61618.1"
                     /translation="MNNDTLIYAKRVIASISQIDTDAGLFRLGKARVDNAKFYLITDS
                     SGVTNLNALLLKINSDTTQSPDSGRFNLQIGNIVLKNSRFKLVQQGSDSLTPETINFQ
                     NLVLSGINLEASEFNLDGDTIGILINGLTAQDHSGFKIDNLRSKITICSKFMHFNNLR
                     LRANGSSLSMNHLLFDFDGWDSFSNFTSRVRINADFEGTYLHTSTLAYIVTELKNYSV
                     GLRVTGQVKGYVDDLRARNLELGFGNISEIALNANLTGLPKIDNTLFSVDLKKLQTSR
                     KDLMQFKVNGSGKPLIDLPEELDRLGLINYVGRFSGYLSDFVTYGTLTSALGAIDFDV
                     WMKPGKTIKSEFKGKASANNFKLGQLLNENLIGSLSFLSSISGTVNKQGELRAFTDAH
                     IQKLEANGYDYKDIDISGNLGNNSYTGTINLNDPNCKLNFLGRVDFSDTIPVFDFSLF
                     APRIDLVKLNLNRVDSVSVASFLLTAKFSGNTLDNSKGEIKLVNSTYRNQRGEFKIAD
                     LTITADNTNESKVITLKSDIAEGEIRSRQSFSRFPYYMNKVLSRHLPALRQDESNGKS
                     KAPQFNAEEYNDYLIKFRLKKTQKVTSILAPDLSIAENSSLFGILDPNKETLTFKLKV
                     LELVTGSTVFKNLSADGETRDSILEANVLMPEIKVGNSYIRNLKVNATTQNNTVRCNI
                     NWNNYSTPSTLGDINLIADFKAFSQPNSTIDINFLPSSLIINDSTWNLSPSLIRIDSS
                     TVQFSNFALSNKHQLISITGKASQLNSDTIAVTLKNIDLSYLNFYLHDSGYKLSGWIN
                     GDALATGILLNPILQAKIGIDKFVVNNQNIGDVKFQSTWHGSENRINLLLSNQRADTL
                     TLLAKGDYFTDKGNLNFDVNINQAKLALIAPLLEGNVSNLQGRMNGSLKIKGDANKPI
                     VDGSIGFDNAGLTIDFLQTHYTLSDKVTINNSNIVLNNFRLTDRFNRTSAINGTIQTN
                     YFKNFNLGLRLNMQNFLCMNTRETDNETFYGTVFASGFVDFLGSPDNLNLNINLKTEN
                     RTAIYLPLSSTSTVEESNFISFVNNNPDLIEIEEAVQNVQTSSSNINITMDLQVTPEA
                     EAQIIIDKKLGDIIKANGSGSLRMEINPSKDVFRMFGRYTIEQGDYLFTLSGVINKRF
                     RIEQGSYIDWNGDPLDANMDIKAVYRVKTSLKQLLLDDSYTARVPVDCKIMLSQKLLT
                     PSIKFGIDFPNLDQQTKALVDGMLNTEEKINTQFLGLLVINSFISDPGMTAAGTQTSN
                     ASLGTTGLYNTASELLSNQLSNWLSQWSKNFDIGINYRPGLENELSSDQVEMALSTQI
                     LDDRVSISSNVGVGGNKNSSNALVGDFTVDIKLNKSGKLRGKAFARNNNDVLLTSQQN
                     NYTTGAGIVYREDFNTIRELFNSIFSKDKQNPNDTLQNDSNTNQNSTNTNLPDTCFVK
                     IN"
     gene            11216..11794
                     /locus_tag="PK049_08405"
     CDS             11216..11794
                     /locus_tag="PK049_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOK61619.1"
                     /translation="MIPIFLLSVAAVYIFFERYFAIRKASVTDVNFMNRIREYIHEDK
                     IDAAIALCRSQNTPVARMIEKGIQRIGRPLPDVNAAIENVGNLEISKLEESLPFLATI
                     AGGAPMLGFLGTVLGMIRAFYDMSMAGNNIDVGLLSNGIYTAMVTTVAGLIVGIFAYF
                     GYNYLVARIEKVVFNLEANTNEFMDLLYEPVS"
     gene            11807..12208
                     /locus_tag="PK049_08410"
     CDS             11807..12208
                     /locus_tag="PK049_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOK61620.1"
                     /translation="MALKRRVKVEPSFSMSSMTDIVFLLLIFFMVSSTLIHPNALKLL
                     LPQSNSQVSAKPITSVSITADRNFYLETVPVTLGQLEYLLQQKMKGQDDPTIALHVDR
                     SVPMEEVVKVMNIAKDNKYKLILATQPLQKR"
     gene            12220..13041
                     /locus_tag="PK049_08415"
     CDS             12220..13041
                     /locus_tag="PK049_08415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOK61621.1"
                     /translation="MTNTEYRVRGIVGTILFHALLVFLLIVFGFSTPLPLPAERGILI
                     NFGNSDDGSGIEEPRVQEASSMPQQVQASKPAEESPLTQTHEEAPSLPTPKKESTKKE
                     QTKSKTETTTPQPTQNTQTTQTNTEETPKVNQKALFPGQKTSGSTTGEGETGKPGNQG
                     GLEGSPESTNRTGTTGGGGDAERGIIANLQGRTAQSLPKPEYPSQKEGRVVVEVTVDN
                     RGNVTKAVAGVRGSTTLDNQLLRAAEKAALNAKFNVDPNAPASQIGTITYIFKLQ"
     gene            complement(13050..13799)
                     /locus_tag="PK049_08420"
     CDS             complement(13050..13799)
                     /locus_tag="PK049_08420"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOK61622.1"
                     /translation="MDFWLFVLVIFVGAAAGFINTLAGSGSLLTLPVLIFLGLPANVA
                     NGTNRIGILMQSIAAVGGFKKKKVFEWSEGIWLTFPAAIGALIGSMVAVDIDELLMNR
                     IIGVLLVFMFVLLLAKPERWLNPTNPKTNIRLTPLRFVVFMAIGFYGGFIQAGVGYFL
                     LAGLVLGAGVDLVKANAIKVLITFVFTVLALGVFIYNNQVDYLIGITMGVGNMLGAWV
                     ATHVAVKKGAVFIRWFLLVCVFAFALKLLLF"
     gene            complement(13801..14280)
                     /locus_tag="PK049_08425"
     CDS             complement(13801..14280)
                     /locus_tag="PK049_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61623.1"
                     /translation="MDTFTIELNEISHKKSKLIRLWIGGALILVVTVALSFYLIGSDI
                     TRLAVLIPVVLQVAIYIYFAYSTYNARVYIQSDAYALEYKFGMLSKTPKTIIWETITK
                     VKIGPTYLAFFKRSGKRTTISLGWLPYVKLKEIKEKICEVAQAKGIACEWAEYKKYS"
     gene            complement(14363..15643)
                     /gene="glyA"
                     /locus_tag="PK049_08430"
     CDS             complement(14363..15643)
                     /gene="glyA"
                     /locus_tag="PK049_08430"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOK61624.1"
                     /translation="MKRDTQIFELIEKERQRQMHGIELIASENFVSPQVLEAMGSVLT
                     NKYAEGYPGARYYGGCEVVDQSEQLAIDRLKQLFDAEYANVQPHSGAQANMAVFMAVM
                     KPGDTFLGLDLSHGGHLSHGSPVNFSGMWYKAVSYGVKEDTGRVDYDMMERMAMEHKP
                     KLIVGGASAYSREWDYKRMREIADKVGALLMIDMAHPAGLIAAGLLDNPVKYAHFVTS
                     TTHKTLRGPRGGIILMGKDFPNPWGLTTPKGEVKMMSQILNSSVFPGVQGGPLEHVIA
                     AKAVAFGEALQPEFKEYQTQVKKNAAAMAQAFIDKGYKVVSGGTDNHSMLIDLRTKFP
                     DITGKKVENTLVLADITINKNMVPFDSRSPFQTSGIRVGTPAITTRGLKENHMPVIVD
                     LIDRVISNIDNEKVIAEVRAEVNKLMKDFPLFAW"
     gene            complement(15713..16108)
                     /locus_tag="PK049_08435"
     CDS             complement(15713..16108)
                     /locus_tag="PK049_08435"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOK61625.1"
                     /translation="MSNPVNNAMGLTGKRILIVDDDTVSRIFLRELLVQSGAVLDFVR
                     NGYELQKYIGSNPEPDVILIDVKLPDADGVELAAQLLAKKPGLRVVAETAYAIEGLDE
                     KCRDYGLKGCVYKPINKDELLKLLLKVAE"
BASE COUNT         4967 a         3752 c         3319 g         4264 t
ORIGIN      
        1 cttcccctac tctactacaa ggcaaccctt ttgcccgaaa ccgaaccggt ttacgacgaa
       61 aacaacgaac aatttgttac cgagagcgat tactcctcgg tagagaacaa ggtggagtat
      121 ttgcttgggc aacataacca gtatgtagag gtaaacgacc cccgagtgga cgaactaacc
      181 ggcctatata ccgcaagtat agccgaatac atggccgata tctaccagga tctaaaaaac
      241 tttgttttgc ggtatcaggt aggaaatacc tatgtgatga acgatgccct ttgggagtgt
      301 atcaataact ttaaggactt ctggggtatc aggttggcta atcttataag agccttccat
      361 atactggcgc atcaaaatat cgaccttgat aatataaagc atcccgatga aagcgagggt
      421 tccgaacgcg atacctccga ctggtttgtt acccgccgcc agcaggatat ggatcataat
      481 gagctaatgt aatgtttgca tctagtatcg ataaggaaac cctaaattct ttacctcgaa
      541 tccattttac ggggcaaatt gttgttgccg aaaaggagag cgatttgtta caatggttac
      601 ccatgttatg ccaggagaaa ataattggct tcgatactga gtcgaaacca tcgtttaaga
      661 agggtaagat aaatggaatc tcattgctgc agctggcaaa cagcaagttg gctattcttg
      721 taaggctaaa gaaaacaggc atccctgata cccttaaaga atttttggag aaccccgaaa
      781 tagttaaggt tggcgcagcc atacacgacg atttaaaggg actactaaaa ctatatcgct
      841 ttactccaaa cggatttatt gaccttcaac gtatagcacc aacctatggc attaaggagc
      901 tgagtgtaaa aaaactctcg gcggtggttc tgggagctac tgtttccaag gggcaacagc
      961 tcagcaactg ggaagccgat gtgcttaccg aagcacaaaa aatatatgca tcgaccgatg
     1021 cttgggtatg cagggaaatc tatcttaaac tcatatcaca caccccgaaa caagagcatt
     1081 aatggaacga cccagaataa ctctcaaacc cggtaaagag caatcgcttc tgaggtttca
     1141 cccatggata ttttcaggcg caattaaatc ggtaaccgag atgcccaacg aaggacaggt
     1201 ggttgatgta ttaggctccg atggtaagtt tttggctact ggacactttg cctcaggatc
     1261 aattgctgtc agggttttat cgtttcaacc cgttgaagtt gatagaacat tctggttaag
     1321 ccgaataagc aatgccctta accttcgcag ggccataggc ctatatccaa ataccgatac
     1381 aaatattttc cgcttggttc atggtgagag cgacctttta cctggactaa tagttgacat
     1441 ttatggcgat actgccgtaa tgcagtgcca ttcggttgga atgtacctgg cgcgcaccga
     1501 aattgccaac gaaataatgc aagtcctccc caataccgta accgccgttt acgataaaag
     1561 ttcaggaaca ttaccatacc gtgaaaaata taacccatcc gatgggtaca tcagcggtgc
     1621 tgataacgcc agggtaccct atatggaata tggcaataag tatcacatat cgtggactga
     1681 tggccaaaaa acgggttttt ttatcgacca gcgcgagaat aggttgctgc tgcaatatta
     1741 cgccaaaggt aaatcggtgc tcaacacatt tggatacaca ggaggttttt cggtatcggc
     1801 cctaaggggt ggagccacag aggttattac ggttgatagt tcggcgcagg ctattaccct
     1861 ggccaaccag aacattgagc taaactttgg tgctaacgca ccacatgctg gcattgcgat
     1921 ggacaccttt gagtatttaa gaacctgcaa ccgcaccttc gatatcattg cactcgatcc
     1981 gcccgccttt gccaagcata acgatgccct acgtaacgca ttgcaagcct acaaaaggtt
     2041 aaaccttgct gctatccgaa gattaaaccc tggtggattg cttttcacat tctcctgctc
     2101 acaggtggtt aacaaaaccg atttcaggaa tgcagtattc tcggcggctg ccatttcagg
     2161 tcgccaagta agcattattc atcagctaac acaacctgcc gatcatccaa ttaacatata
     2221 ccatccagag ggtgagtacc tgaaaggact cgtgttgagt gtgatttaaa ttatcatctt
     2281 acaaaaaccc acttagtagc ggtagaaaga agatcgctgt aactataccc ctcgtagtcg
     2341 aagagtttta aatcttcgac attaaaaatt ttgttcttta ccgcaaagcg tgccataagg
     2401 ccgcgagccc tctttgaaag tattggaatt acctttagcc cctcagggcg attctccttg
     2461 aattcaatgg taataaaccg atcaccaaaa tgcttatcgt taatagccct ggaatactcc
     2521 tttgaagcaa ggttaaccaa atacccatca cttacattct ggtcaagtaa ccccgtaatg
     2581 ctatcacccc aaaaatcgta aagcgattta tgttgcccaa cctgaatctt gcttcccatt
     2641 tctaaacggt atggttgcat gagatcaaac gccctcaata ctccgtataa tcccgagagc
     2701 ataaaaatga ttcgattgga aaatgcaagt tcatcatcag taaaatcggt agcctgtaat
     2761 ccttcataca aatcgccggc aaaggtaagg attgcaggtg tgctattttc cggagtaaaa
     2821 ggcaaatgcc aattagcata ccttgtgaaa ttgaggtcag caatatcttg actaacacct
     2881 aatatatctt ttagctcaac tgggctataa gtctttagct gtttcacaag ttcctgagcc
     2941 ctatcaagaa aaacaggctc agagtaattt ttacctgcca cttttaaatt gcttctcagg
     3001 gtttttgcag gcgaaagcac tatgcagtag ttcgtgtaat ttcccatatc tcatcatttt
     3061 ttgttttttg tttcaatttc agaaattttt tgtatcttta aattagaaac aaagtagagc
     3121 atactgggtt caaaggatga gtaataatag cttaccagtt tttaaaatta gtaaaaatgt
     3181 ttgccacaat tggtggactg ccatatttat tgtcatttcc tttatagctc ttgctttacc
     3241 tttacaaagt ttttccagac ccggtacaaa ggttgtactc cagctcaagt ggaagcatca
     3301 gttccagttt gctgggtact acgctgctat tgaaaaaggg ttttacgctc aggaagatct
     3361 tgatgttgag ctcaaggagt taccccaaaa cacaaatgtg attaccgagg ttcttgaagg
     3421 tagggcccag tttgggatag ccaatgccga cataataata cattacgtta atggagcccc
     3481 tgttgttgtg cttgcaccca tattccagtc aagcccggcc gcccttgtgt cacgagccga
     3541 gcgtaatatt accagaccac aggatattgc tggcaaaacc attgaaataa atgcaaaaaa
     3601 atcgggttcg gttgaagtgc ttaccatgct tagccttgag ggtgttaaac cctcgcagta
     3661 ccaagtaacg gaatcgtcgc tttcacttaa caaactgctg aataaccaaa ccgatgccag
     3721 tgaggtttac ctgacaaatg aaccctattt cctcgaaaaa tttgggatac cctataacct
     3781 gattctgccc cataagtatg gcgttgactt ttacgccgaa tgcttattta ccaccaagga
     3841 gatgattagt aaaaatcctg aactggttga tgctttcctt agagcatcca ttaaaggatg
     3901 ggagtacgcc cttgaaaatt cggaggaaat agcgcaggta attcaatcaa agtataaaac
     3961 cacaaaaact cttgatcacc tcatattcga atcgcaaaag gtgaaagagt ttattcaacc
     4021 taactttata aatgttgggc attcaaacaa aggccgatgg ctacaaatga tggaaaccct
     4081 tttacaggcc ggattaatta acaaaacaaa acctattgat gctttgcttt acaacccaca
     4141 gcaaaaaaaa cattcacctt ttaccgataa atggtttatt ttggttattg gcacattatt
     4201 gctgctaact ttcatattac acttttggta tattagtagg gtaaataagc gcaagcaaca
     4261 attagtaaac acccacaagc aacaggttga gagcttaaag caagagttac agatgttaaa
     4321 cacccatctg gaagagactg ttcaacgaaa caaagacctt gaatcgttta aggaatcgct
     4381 actctctaac ttttcgtttg agattcgaac acccctcaat aacattattg gctacagtga
     4441 acttttaaac gannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntatcggac
     4561 cgaaaccttt acaagagtat attccaaagg ctaattaaca atgctgtaaa gtttacgtcg
     4621 aaaggttaca ttgagattgg cgtaagaaaa tcggaaaaaa atggtcacct ccttttctgg
     4681 atacaggata gcggaacagg aatagagatt gaaaaatcaa aaacattatt taaccgttta
     4741 aatattgaag agtcgcgcga tgggtttggt gtactacaaa tgggactccc tgttgttaag
     4801 ggtattgtgg atctgctgaa cggaaaaatt tgggttgaaa cagctgaggg tactggaact
     4861 actgtgaatg ttgaagttcc ctacacgcca attggcagcc acaaagcaaa aacatatcat
     4921 aaaaaatcac cattctacat acatcaagac cctccaaagc tgaaaaataa aaagattctg
     4981 attgttgaag atattcaatc gaactacatt ctgctaagca gactcctgga agaaactggt
     5041 tgtaagctag agcatgccaa aaccggagac gaggcactca aaaaggttga aaacaccccc
     5101 gactttgacc taattttcat ggatttaagg ctagctgaca tggatggtat tgagattacg
     5161 cgccaaatcc gaaagaagaa caccagggtg gttattgtgg cacaaaccgc ttacagttca
     5221 ggagtaaaag taaacatgag cattgaggca ggctgtaacg attttattac caaaccaata
     5281 agccgcatcg acctttacaa tgttcttcgt aagtaccttg taaccgacga tcacacagcc
     5341 taacccaggg gtaaccgagc taaaggaggc tcatcaagcg gcgatgtctt tccagcaagg
     5401 tagctgtagt aacccacaat ggcaatcatt gcagcattat cggtagtaaa ctgcttctta
     5461 ggtaggtaaa ccttccatcc cctcttttgg ccaagtagct gtaaagcggt tcgtaatcct
     5521 gaatttgctg ccacgcctcc agcaatggca atttccttta tcctgtactt acgtacagcc
     5581 tgctcaacct tacttagcag tatctcaata atggtttgct gaagcgaggc gcaaatgtcg
     5641 ggtaggtgtt cgttaacaaa gtttggttca gtgtttagtt tatcgcgcag ggtataaagg
     5701 aatgatgttt ttaatccgct aaagctaaaa tcgaggtcgg gtatattcgg cttggcaaac
     5761 tttatgaatc cagggttacc ctcgatagca agtttgtcaa ttacaggccc accagggtaa
     5821 ggcagcccaa gcaccttggc acacttgtcg aatgcctcgc ccgctgcatc atcaattgtt
     5881 tggccaacta tttccatatc cattggaccc cgaaccaaca tgatttgggt gtggccccct
     5941 gagaccaaaa ggcataaaaa ggggaacgat gggtgaatag cattgggctc atccttaatg
     6001 aagtgcgaaa gcacatgacc ctgtaagtga ttaaccgata ttattggaac tcccagtgtg
     6061 agcgccgctc cctttgcaaa cgaagcccct acaagcaacg agcccagcaa ccccggacct
     6121 cgcgtgtagg ctattgcatc tatctccgca atggtaaccc cagcgtaacg cagggcagta
     6181 tctactacag gaatgatgtt agcctgatga gcgcgcgagg ctaactcagg cacaactcca
     6241 ccatactgtt tatgaacatc ctggctgcta attacattcg ataaaagtag ctgatcgcgg
     6301 ataactgctg ctgaggtatc gtcgcacgat gattctatcc caagtattgt tacgctcatc
     6361 aataaaaatt gaaataatac aaaaaaagtt taaaaaccta tcctcaccta tttcaaaagc
     6421 ccaagcattt gtatatttta ggaacaaatt ttgatgccca cattccagaa ataacttagc
     6481 aaaggtatta aaaaactact taaaatactg atttgggggt ttgttacact actcgcaatc
     6541 cctacgcttg cttggctgct gctgcaaagt agccaggtac aaacctatat tattaataaa
     6601 actacccatt atttacacca aaaaacagga attactgtaa acattagaaa agttgatgtt
     6661 aggccattta caggcatttt actaaaagat gtttacgttg ccgatttgaa caacgacacc
     6721 ctaatctacg caaaaagagt aatagcatcc atttcacaga ttgataccga cgccgggctt
     6781 ttccggttag gaaaagcaag ggttgacaat gcaaagttct accttattac tgatagttca
     6841 ggggtaacaa acctaaatgc tctcctgctt aaaattaatt ccgacaccac tcaatcgccc
     6901 gattcgggca ggtttaatct gcaaattgga aatattgttc ttaagaactc ccgttttaag
     6961 cttgttcagc aagggtccga cagccttacg ccggaaacaa taaactttca gaatcttgtt
     7021 ctttcaggaa tcaatttgga agcaagcgag tttaacctgg acggcgatac cattggaata
     7081 ctaataaatg gtttaacggc acaggaccat tccggattta aaatagataa cctgcgaagt
     7141 aagattacca tttgcagtaa gtttatgcat ttcaataacc tgcgcctgcg ggcaaatggc
     7201 tcgagcctat ccatgaacca tcttttattc gacttcgacg ggtgggatag cttcagcaat
     7261 tttacttcac gggttagaat aaatgccgac tttgaaggca cctacctgca taccagtacc
     7321 ttggcctaca ttgttaccga acttaaaaat tatagcgttg ggttaagggt aaccgggcaa
     7381 gttaaaggct atgtggacga tttaagggca cggaaccttg aactgggctt tggcaatatt
     7441 tcagagatag cccttaacgc caacctgacc ggcttaccca aaattgataa tacccttttc
     7501 tcagtggacc tgaaaaaact tcagacttct agaaaagatc tgatgcaatt caaggtaaat
     7561 ggtagtggaa aacccctaat cgatttgccc gaagagcttg atcgccttgg gctaataaac
     7621 tatgttggaa gattctccgg ctacctcagc gactttgtta cctacggtac cctaacatcg
     7681 gctctagggg caattgattt tgatgtttgg atgaaacctg gcaaaaccat taaaagtgaa
     7741 tttaagggca aggcatcggc taacaatttc aagcttggcc agctcttaaa cgaaaattta
     7801 atcggaagcc tgtcgttcct atccagcata tccggtacag tcaataaaca aggagaactt
     7861 agagctttta ccgatgccca tattcagaaa ctcgaggcta atggatatga ttataaagat
     7921 atcgatatat ccggtaatct agggaacaac tcatacaccg gaaccatcaa ccttaacgac
     7981 cccaactgta agcttaactt tttgggtagg gttgactttt ccgacaccat tcccgtgttc
     8041 gatttctcac tctttgcccc tcgcatcgat ttggttaagc tcaaccttaa cagagttgac
     8101 tccgtttcag tggcatcgtt ccttttaacc gcaaagtttt ctggcaatac cctcgacaat
     8161 agtaagggcg aaataaaact ggttaactct acctaccgta accaacgcgg cgagtttaag
     8221 attgccgacc ttacaattac tgccgataac accaatgaaa gcaaggttat tacgcttaaa
     8281 tccgatattg ccgagggtga aatacgtagt aggcaaagct tttctaggtt cccctactac
     8341 atgaacaagg ttctgtcgcg ccacttacct gcccttaggc aggatgaatc caatgggaaa
     8401 tccaaagctc cacaatttaa cgctgaggag tataacgatt acctcatcaa gtttaggtta
     8461 aaaaagacac aaaaggttac aagcatttta gcgccagacc taagcattgc cgaaaactca
     8521 tcgctttttg gcattttaga tcccaacaag gaaaccctaa cctttaagct taaggtactt
     8581 gaactagtaa caggcagcac cgtttttaaa aatctaagtg ctgatggcga aacccgcgat
     8641 agcattctgg aagccaatgt gctaatgccc gaaataaagg ttggcaacag ctatatccga
     8701 aaccttaaag taaacgctac tacccaaaac aacaccgtaa ggtgtaatat taactggaat
     8761 aactactcaa caccttctac cctgggcgat attaacctga ttgccgactt taaagcgttc
     8821 tcgcaaccta acagcacaat agatattaat tttctaccct catcgcttat tataaacgac
     8881 tccacatgga acttgtcgcc atcgctgatt cgtatcgatt catctaccgt acagttttcc
     8941 aattttgccc tttccaataa gcaccaacta attagcataa ccggtaaagc gtcgcagctt
     9001 aattcggaca ctatagcagt aactctcaag aacatcgacc tatcatacct gaacttttac
     9061 ctgcacgatt ctggttataa gctgagcggt tggataaatg gcgatgcctt ggcaactggc
     9121 atcctgctaa accctatact tcaagccaag attgggattg ataagtttgt agtaaataat
     9181 caaaatattg gagatgttaa attccagagc acctggcacg gtagcgagaa taggattaac
     9241 cttttactaa gcaaccaaag ggccgatacc ctaacactcc ttgcaaaagg cgattacttt
     9301 accgataaag gcaacctcaa ctttgatgta aacattaacc aggcaaagtt agccctaatt
     9361 gctcccttac tggaagggaa tgtttccaac ctacagggcc gcatgaatgg ctcacttaaa
     9421 attaaaggcg atgccaataa acccatagtg gatggtagta ttggctttga caatgccgga
     9481 ctaaccattg attttctaca aacccattat accctatccg acaaggtaac cataaacaac
     9541 agcaatattg tattaaacaa tttcaggtta accgataggt ttaatcgaac atcggctata
     9601 aacggaacca ttcaaaccaa ctactttaag aatttcaacc ttggacttag gttgaacatg
     9661 caaaatttcc tctgcatgaa cacccgtgaa accgataacg aaacgtttta tggaactgtt
     9721 tttgcctccg ggtttgtgga ttttttgggt tcgcccgaca acctaaacct caatataaac
     9781 cttaaaaccg agaacagaac agcaatttat ttaccccttt cctccacaag cactgtggaa
     9841 gaatcaaact ttatcagctt tgtaaacaac aaccccgatt tgattgagat tgaggaggca
     9901 gtgcagaatg ttcaaacctc atcatccaac atcaacatta ccatggattt acaggtaacc
     9961 cccgaagccg aggcgcaaat tattatcgac aagaaactag gcgatattat taaagcaaat
    10021 ggcagcggta gcctgcgaat ggaaattaac ccgtcaaaag acgttttccg tatgtttggc
    10081 cgctacacca ttgagcaggg tgattacctt ttcaccctct ctggggttat caataagcgt
    10141 ttccgcattg agcagggcag ttacatcgat tggaatggcg acccgctaga tgctaacatg
    10201 gatatcaagg ccgtgtaccg ggtaaaaacc tcacttaagc agctcttgct cgacgatagc
    10261 tatacagccc gtgttccggt tgattgtaaa atcatgctat cgcaaaagct ccttacacca
    10321 tcaatcaagt ttggtatcga cttccctaac ctcgatcagc aaaccaaggc tttggtcgac
    10381 ggaatgctca ataccgagga aaaaataaat acccagttcc tagggctgct ggtaataaac
    10441 agcttcattt ccgaccccgg aatgaccgcg gctgggacac aaacctccaa tgccagcctt
    10501 ggcactaccg gcttatacaa tactgccagt gagttactct ccaatcagct aagcaattgg
    10561 ctctcccaat ggagcaaaaa ctttgatata ggtataaact accgcccggg tctggagaac
    10621 gagctcagct ctgaccaggt tgaaatggcg ctatcaaccc aaatactgga cgatagggtt
    10681 tccattagta gtaacgtggg tgtgggtggc aataagaact ctagcaatgc acttgttggc
    10741 gactttaccg ttgatattaa gctcaacaaa agtggtaaac tccgtggtaa ggcctttgca
    10801 cgtaacaata atgatgttct gctaacaagt cagcagaaca actacaccac tggtgcaggt
    10861 attgtttacc gtgaggattt taacaccatc agggagcttt tcaactcaat cttcagcaag
    10921 gataaacaga atccaaacga tactttacaa aacgatagca acaccaacca aaattctacc
    10981 aatacaaact tgcccgatac ttgctttgtt aagataaatt agcactgtgc tcacctattt
    11041 gaacaatgaa aggtggaata atacgttttt ttactaattt tcggaaaaat ttaaaataat
    11101 gaatacgatg ctatccatgt ttgttcttct tcaaactcct gccggtgtta atcccggaac
    11161 gcaaggtgaa atatctttaa gttttctgga tcttgccctt aaaggcggat ggataatgat
    11221 tccgatattt ttactatcgg tagcagccgt ttacatcttt tttgagcgct actttgccat
    11281 tcggaaagcc tcagttaccg atgtcaattt catgaaccgt atcagggagt atatacatga
    11341 ggataaaata gatgctgcta ttgccctttg ccggtcgcaa aacacaccgg tagcccgcat
    11401 gatagagaag ggaatacagc gcattggccg cccgctgccc gatgtaaacg cagccattga
    11461 gaatgttggt aacctggaaa tttcaaagct cgaggagagc ttgccattcc tggcaaccat
    11521 tgctggcggt gcccccatgt taggattcct aggaactgtt ctaggtatga tccgtgcctt
    11581 ttacgatatg tccatggctg gaaataacat cgatgtgggc ttgctctcaa acggtatcta
    11641 caccgccatg gtgacaaccg tagccggttt aattgttggt attttcgcat actttggata
    11701 caactacctg gttgcccgta tcgaaaaggt tgttttcaac cttgaggcca acaccaacga
    11761 gtttatggat ttactctacg aacctgtttc gtaaaaattt acccctatgg cactcaaacg
    11821 aagagtaaag gttgaaccga gctttagcat gtcatccatg accgatatag tattcctatt
    11881 acttatcttt ttcatggtat cgtcaaccct tattcacccc aatgcgctaa agcttttact
    11941 accccaaagt aacagccagg tatcggcaaa gcccataacc tcggtttcca taactgccga
    12001 taggaatttt tacctggaaa cggttcccgt taccctcggg caactggaat accttttgca
    12061 gcaaaagatg aaaggacagg acgatcctac cattgcacta cacgttgata gaagcgtacc
    12121 catggaggag gtggttaagg ttatgaatat tgccaaggat aacaagtaca agctaattct
    12181 ggcaacccaa ccattacaaa aaaggtagta agaataaaaa tgacaaacac cgaatataga
    12241 gtaagaggaa tagtgggaac aatactgttc catgcacttc ttgtattcct attgatagta
    12301 ttcggtttta gtaccccgtt gccattgccg gccgagaggg gcatcctaat aaactttggt
    12361 aattccgacg atggtagtgg aattgaagaa cctcgtgttc aggaagcatc gtcaatgcca
    12421 caacaggtac aggcaagcaa acccgctgag gaatcgccat taacccaaac ccacgaggag
    12481 gcgccatcgc ttcctacccc aaagaaggaa tcgaccaaaa aggagcaaac taaaagtaaa
    12541 accgaaacca ctactcccca acccacccag aacacccaaa ccacccaaac aaatactgaa
    12601 gaaacgccaa aggtaaacca aaaggctctt ttccctggtc agaaaacctc cggtagcact
    12661 accggtgagg gtgaaaccgg taaacccggc aaccagggtg gacttgaagg atcacctgaa
    12721 tcgaccaacc gtactggaac tacaggcggt gggggcgatg ctgaaagggg cattattgca
    12781 aaccttcagg gcagaaccgc ccaaagcctt ccaaaaccgg aatatccatc gcaaaaggaa
    12841 ggacgtgttg tggttgaggt tactgtggat aaccgaggaa acgtaacaaa ggctgttgca
    12901 ggggttaggg gttcaactac gctcgataat cagctactac gtgccgctga aaaggcagcc
    12961 cttaatgcca agttcaatgt tgatcccaat gctcctgctt cgcaaattgg gacaataact
    13021 tacattttta aacttcagta attcaagggc tagaatagaa gcaattttaa ggcaaaagcg
    13081 aacacgcaca ccagcaaaaa ccaacgaata aacacggcac ccttcttaac agccacatgg
    13141 gttgcaaccc aggcccctaa catatttcct acccccatgg taatgccaat caggtaatct
    13201 acctgattat tgtatataaa cactcctaaa gccaacacgg taaatacgaa agtaatcaac
    13261 acctttatgg cgttagcctt aaccaaatcc acgcctgcac caagaactaa cccggccagt
    13321 aaaaagtaac ctacaccggc ctgaataaaa ccaccataga agccaatagc cataaatact
    13381 acaaaccgta agggagttaa acggatgtta gttttggggt ttgttgggtt gagccatcgt
    13441 tcaggcttag cgagtaataa aacaaacata aacaccaaaa gcacaccaat tatacggttc
    13501 attaggagtt catcgatatc aaccgccacc attgaaccaa ttaaagcgcc aatagctgca
    13561 gggaaggtaa gccaaatacc ctcgctccat tcaaaaacct tctttttctt aaacccgcca
    13621 acagcagcaa tgctctgcat taaaatccca attcggttag tgccattggc tacattggct
    13681 ggcaagccaa gaaaaatgag tacgggtaga gtaagcaacg acccacttcc tgccaaggta
    13741 ttgataaatc ctgctgctgc cccaacaaaa attacaagga caaaaagcca aaaatccatc
    13801 ttagctatac tttttgtatt cagcccactc acaggctatg ccctttgcct gggcaacctc
    13861 gcaaatcttc tcttttatct ccttcagctt aacgtatggg agccagccca agctaatggt
    13921 tgtgcgcttg cccgaacgct tgaaaaaagc taggtaggta gggccaatct taactttagt
    13981 aatggtttcc cagattatcg tttttggggt tttgcttagc atgccaaatt tgtactcaag
    14041 agcataggca tcggactgga tatatacacg tgcattgtag gtagagtagg caaagtagat
    14101 gtaaatggca acctgtaata ctaccggaat aagaacagct aaacgggtaa tatcgcttcc
    14161 tattaagtag aacgaaagcg caactgtaac taccaggatt aaagcaccac caatccaaag
    14221 cctgatcagc tttgattttt tatggctaat ttcgttaagt tcaatggtaa aggtatccat
    14281 agccagctaa tttttcatgc aatttactaa aaaaccccaa tagcctatgc cattggggtc
    14341 aaattattta ggttgatacc gattaccagg caaagagtgg gaaatccttc attagcttgt
    14401 ttacctcagc ccttacctcg gcaataactt tctcgttatc aatattggat attaccctgt
    14461 caatcagatc cacaataact ggcatgtggt tttcctttaa accacgggtg gtaatggcag
    14521 gagtaccaac acggataccc gaggtttgga atggggaacg gctatcgaaa ggaaccatgt
    14581 ttttatttat ggtgatatcg gctagcacca gcgtgttttc caccttttta ccggtaatat
    14641 cggggaattt agtacgcagg tcgataagca tagagtggtt atcggttcca cccgaaacca
    14701 ccttgtagcc tttatcaata aaggcttgcg ccatagcggc agcattcttt ttcacctggg
    14761 tttgatactc cttgaactca ggttgaaggg cttcgccaaa agcaactgct tttgctgcaa
    14821 taacatgctc aagagggcca ccctgaacac cagggaaaac actggagttc agtatctgcg
    14881 acatcatttt cacctcaccc ttaggagtgg ttaaacccca tggattgggg aaatccttac
    14941 ccatcagtat gataccaccg cgggggccgc ggagggtttt gtgagtggta gatgttacaa
    15001 agtgagcgta tttcacagga ttatcgagca aaccggcagc aattagacct gctgggtggg
    15061 ccatgtcaat cattagcaat gcgccaacct tatcggcaat ctcgcgcata cgcttgtagt
    15121 cccactcacg ggagtaagcc gaggctccac caactataag ctttggctta tgctccatgg
    15181 ccatgcgttc catcatgtcg tagtcaaccc taccggtatc ttcctttaca ccgtacgata
    15241 ccgccttgta ccacatccct gaaaagttaa cgggcgaacc gtgagagagg tgaccgccat
    15301 gcgaaaggtc gagtcccagg aaggtgtcgc cgggtttcat cacggccatg aaaacggcca
    15361 tgtttgcctg cgcccccgag tgaggctgaa cgttggcata ctcggcatca aatagctgtt
    15421 ttagcctgtc aatggcaagc tgttccgact ggtcaaccac ctcgcaacca ccgtagtagc
    15481 gtgcaccggg atacccctcg gcatacttat tggttaacac cgaccccata gcctcaagaa
    15541 cctgagggct aacaaagttt tccgatgcaa taagctcaat gccatgcatc tggcgctggc
    15601 gctccttttc aatcagttca aaaatctgag tatcgcgttt cattctatta gtatttaaaa
    15661 ttatgccgta aaattaacct tttaaaaata attcaaatac gagcgccatg tgttactcag
    15721 caacttttag taaaagtttt agaagctcat ccttgttgat tggcttgtac acgcacccct
    15781 ttaaaccgta atcacgacac ttttcatcga gcccttcaat tgcataggcg gtttcggcaa
    15841 caacccttaa gcctggcttt ttagccaata gctgagctgc aagctcaact ccatcggcat
    15901 cgggtagctt aacatcaata agtataacat cgggctccgg gtttgagcca atatattttt
    15961 gcaactcata accattacgg acaaaatcca acactgcacc cgactgcact agcaattccc
    16021 taaggaatat ccggctaacc gtatcgtcat caacaatcaa aatcctcttt ccggttaggc
    16081 ccatggcatt attaactgga ttggacatgg ttttgggttt ttagtcaact tatttttacc
    16141 aaatataatg ttaatttact gaatttcaaa atagtgacaa tttgttactc aaagttgatt
    16201 aatacaaccc cctgtcgttt agctcgggat tttattatgc tgtatcaatg tcgtttagtt
    16261 aacaaaaata tacatgcctg tcatcctaag cggcctgtcc cgccctagcg gggagtgaat
    16321 gatctctaat cgaccccatg agaagtatcg ctcgcgttca acttgacaat agcttcacca
    16381 acaacgattc acgaacaacg aa
//
LOCUS       DAOSAL010000058        16092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_2497_length_16092_cov_48.941386, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000058 DAOSAL010000000
VERSION     DAOSAL010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 16092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16092
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2497_length_16092_cov_48.941386"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /locus_tag="PK049_08440"
     CDS             <1..860
                     /locus_tag="PK049_08440"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005642224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOK61626.1"
                     /translation="GKTEAASRIVAITDQSKGALRKLADSEGYRTFVIPDDVGGRYSV
                     LTPVGLLPIAIAGFDIRQLVEGARHAMNDTSANVPFEQNPACQYAAARNVLYANGKKV
                     EIMVNYTPKLHFVTEWWKQLYGESEGKENKGIFPAGVDFTTDLHSMGQYIQEGERFIF
                     ETVLSVEKPWHKLTIPNDPDNLDQLNFLTGKRLTEVNHMAELGTIVAHNDGGVPCIQI
                     TIPELSAYWIGYLLYFFERACAISGYTLDVNPFDQPGVEAYKKNMFALLGKPGFEDEG
                     KALRKRLGI"
     gene            complement(927..1127)
                     /locus_tag="PK049_08445"
     CDS             complement(927..1127)
                     /locus_tag="PK049_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61627.1"
                     /translation="MEITRAVLAIGRQWVTNSGFTMDLFIGGLYSKGGIETEEGATGE
                     NFDVAKFEGFRIRTEFSIGFAF"
     gene            complement(1464..3884)
                     /locus_tag="PK049_08450"
     CDS             complement(1464..3884)
                     /locus_tag="PK049_08450"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HOK61628.1"
                     /translation="MQKRAIYLGLVTVWLLLLTSYSLLFGVPKKYVPMVVYQPDGTKI
                     ECFASGDEFHNWLHDADGYTIIQHPKTGYYVYAAKENGKFVASDFVVGKVKPTATKGL
                     EPFTNISREEYIAKRKRKRVNNHYLSLTPTKGADGIAPLRAKDTKEATVMNNIVIFIR
                     FADSNPDDEPIDYYQNIYNGASMSVSDYYNSVSYGKFTISTTFYPTTTNNYVVWYQDS
                     QNRNYYRPYNATTNPIGYDPNDDNYSSSKSQTYREHTLLKNAIEAVASQIPQNINLDI
                     NEDGYVDVVSFIVAGDNDGWNELLWPHQWSLWSKAVRINQKQVGDYTFQLQLFNNARI
                     DLGTLCHEMFHALGAPDLYHYDDNLNRTPVGVWDIMEGTKDEPQNMGAYMKYLYGGWI
                     DNIPVINQTGTYTLNPLSTSATNNSFIIPSPATHTEYFLVEYRKREAYDASLPNDGML
                     VYRINSKFEGMGNADGPPDEVYVFRPNGSLTLNGTINSATFNADYGRTTMNNQTNPHS
                     FLSDGSNGGINITNVTSAGSTISLNATIDFSPWIVLKNDRGYGSAIGNVSSTLTVATR
                     FTASDMANYVGKYIKKVDFFLRGNDNGQRLTSGEVVKIWEGGTVGNPGTLVYSQGASA
                     EVKVDEWTTHNVTTPVEIKANKEYWVGYTATASGGYPFATDRGPMVPDKGGWFLNGGQ
                     WVQLAQFNLDYNFLIRAIVSDKTTDVPVNSAEENAISFYPNPAENDGYIKFFNNQNSV
                     NANIEVVNMLGQVIISQSISNLSAGKNVMSVNLSGLSKGIYIVKISMINPASLQVIST
                     KTIKFNKK"
     gene            complement(3924..4463)
                     /locus_tag="PK049_08455"
     CDS             complement(3924..4463)
                     /locus_tag="PK049_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61629.1"
                     /translation="MNRIITSGVVFTLLLSCASNTKMEKEQGKNDLVPFVEFYSITEG
                     KALSGVPYQGRRIDGPGYSYDPKTQKLDIYRNNITDSLSIKLYLGVGKVLKGTAGQGV
                     SSTIIGVNEYPFSYNEFTITNATNYKVNCYFKGKQFVLKPNQEYLITETKIDSLPNAT
                     VLQTTTTWKVTFTGFVKNK"
     gene            complement(4585..5910)
                     /locus_tag="PK049_08460"
     CDS             complement(4585..5910)
                     /locus_tag="PK049_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010992147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOK61630.1"
                     /translation="MKAMRYILGGLCLVLTFNTPVFAQVENQSLNSDSIYEQRQINQM
                     DSLLYQWYIQQPNEDNNLVADIEGDTIEGPELPDSFYIKRLQTINSLIDLPYNNIVRS
                     FIKAYTEKKRDKVEVMLGLTDYYFPMIEEILDMYQLPQELRFLPVIESALNPRAVSRA
                     GATGLWQFMYGTGRMYNLTINSFIDERRDPLAATHAACRFLKDLYSIYGDWTLVIAAY
                     NCGPGNVNKAIRRSGGKRNYWDIYYHLPRETRGYVPAFIAANYTYYFYKEHNLQPKPI
                     TLPPATDTIIVNNMLHLQQIAEVLDYPIELLRDLNPQYKIDIIPAKGKSFVLRLPKEM
                     VGPFLDREKEIYAYKDSIFFNQKILANPSKYTASYQHEVPPGSIRIVYRVKKGDVLGS
                     IAQRYNVSVRQLKGWNNLHRDLIRENQRLVIYVPERTATRLGIVQEKKS"
     gene            complement(6024..6536)
                     /locus_tag="PK049_08465"
     CDS             complement(6024..6536)
                     /locus_tag="PK049_08465"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOK61631.1"
                     /translation="MKIFLIGFMSSGKTTVGRELAHFLGYQFLDLDEYIETKHNRTIK
                     QIFETKGEEHFRLIENEALKEVCSFNGDFVISSGGGTSCFYNNIDYMNRNGITVYLRL
                     EVSTLVSRLVESKIDRPLLWGKTREELNDYIIRVLDERKKFYEKAQIIVDADNTKPID
                     LAKLIISALE"
     gene            complement(6650..9304)
                     /locus_tag="PK049_08470"
     CDS             complement(6650..9304)
                     /locus_tag="PK049_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HOK61632.1"
                     /translation="MRLDDFEADELLSEGEGKPVSPELHAKIERITGEAAKKTHITGM
                     YKDWFLDYASYVILERAVPHLDDGLKPVQRRILHAMKRLDDGRYNKVANIIGYTMQYH
                     PHGDASIGEALVQLGQKDLLIDTQGNWGNILTGDSAAAPRYIEARLSKFALEVVFNPK
                     TTEWMLSYDGRNQEPVTLPVKFPLLLAQGVEGIAVGLASKILPHNFNELIDAAISYLK
                     GKDFELYPDFPTGGLADCSRYNDGLRGGAVKVRARIQKLDRKTLAITEIPFGKTTTSL
                     IESILKANDKGKIKIKRIDDNTAENVEILIHLHPDANPDMTIDALYAFTDCEMSISTN
                     SCVIYDDKPRFMGVKDILRASVDRTKELLTMELNIRLAELEEDWHFSSLEKIFFEERI
                     YRELEKDTETWDLVIEAIERGFDPYRKLLRREITRDDILKLTEKPVRKISKFDIKKAD
                     EHIKAVEAEMEQVKHHLANIVTYTIDYYQQIKKKYGKGRDRKTELRSFDTIVATKVVV
                     ANEKLYVNRAEGFVGTGLKKDEYVCDCSDIDDVIVILRNGKYYIRKVQEKDFFGKDIV
                     HVGVYKKNDTRTIYNVVYRDGLNGDIMMKRCAINGTTRDKEYDITKGTQGSQILWLSA
                     NPNGETEVLKVYLKPRPRLRNLILELDFSQLAVKGRGSQGNIFTRYAIHKIMLKEKIA
                     GAVEGVKVWYDPEVMRLNHEGQGELLGEFLPDERVLVVNPDGTYFLANTNYALRFDVQ
                     PLLVEKFNPHKVFSAVYFDGEQKQYYLKRFRFEPTEKTQSFIPDEYSSKLIALNADVF
                     PALKLVFGGKNANREPENIDVDAFIAVKGYKAKGKRLTTFDVKRVEFIEPLEKERSAF
                     EEETSGIPDSESEDNDSGSSAEQMSLEL"
     gene            complement(9331..11247)
                     /locus_tag="PK049_08475"
     CDS             complement(9331..11247)
                     /locus_tag="PK049_08475"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HOK61633.1"
                     /translation="MSVGYSEDSIKTLEWQEHIRKRPGMYIGKLGDGSQPDDGIYILL
                     KEVVDNSIDEFMMGNGKHIDILVNDKTVSIRDYGRGIPQGKLIDVASKMNTGAKYDSK
                     VFKKSVGLNGVGIKAVNALSNQFIIKSIREGLVREAEFAGGILVKDKQYPANGEANGT
                     YVQFTPDEAVFGQYSFQDEYIETMIRNYTYLNTNLTISFNGKEFISKNGLLDLLNENI
                     TTEPLYPIIHLKGEDIEVAITHGVGYGEDYYTFVNGQHTTQGGTHLTAFREAYVKTIR
                     EFYKKDFDPSDIRTSIIAAISIKVEDPVFESQTKTKLGSKDMGPNGPSVKQFITDFIK
                     EKLDNFLHRNSETAQILLQKIIESEKERKAISGIKKIARERAKKASLHNRKLRDCRIH
                     YNSKDDRAHESTLFITEGDSASGSITKSRDVNTQAVFSLRGKPLNTYGLTKKIVYENE
                     EFNLLQAALDIEEDMENLRYNKIVIATDADVDGMHIRLLLITFFLQFFPELIRQEHLF
                     ILQTPLFRVRNKKKTVYCYTPEEKEKAIAELGPNPEITRFKGLGEISPDEFKNFIGPD
                     MRLDPVRLTKEDQIHDLLEFYMGKNTPDRQDFIINNLRIEEDIIDEEPAPKADENNID
                     EEGNHDVENKNLVA"
     gene            complement(11548..>11844)
                     /locus_tag="PK049_08480"
     CDS             complement(11548..>11844)
                     /locus_tag="PK049_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005819790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOK61634.1"
                     /translation="AEIEQFWLDAYPEIDTIPSKVAIMQKAGYIPVATFILPENCWTE
                     HFYAPQVTAQKLFMEKYAGNQNAMRLVENLLHEAQLYARYKDYYGYVFYIGKKM"
     assembly_gap    11845..11944
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11945..12476)
                     /locus_tag="PK049_08485"
     CDS             complement(<11945..12476)
                     /locus_tag="PK049_08485"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007481293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOK61635.1"
                     /translation="MEQENNSIHDFDIALICEYFSYVERQGPGSPEVTIKALSFIDNL
                     TQNAKIADIGCGTGGQTMVLAQNTPGTIIGIDLFPQFVDIFNQNATKLGLTDKAKAIV
                     GSMDNLPFQVEELDLIWSEGAIYNIGFERGLTEWRRFLKKGGYVAVTEASWFTPERPA
                     EIEQFWLDAYPEIDTIP"
     gene            12885..>13332
                     /locus_tag="PK049_08490"
     CDS             12885..>13332
                     /locus_tag="PK049_08490"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOK61636.1"
                     /translation="MPRTKQQVEAIRQQRVMQIFDAALDVFAHKGFHSATIADIAQKA
                     GIAKGLLYHYYSSKEELLSHIVINGMNNLIEKFDENHDGVLTDAEMELFINDMFRKIV
                     ENRNYWKLYFSVMLQPNVFSKELMQKLEGIYEPMVSILIQYFRRKGY"
     assembly_gap    13333..13432
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13433..13813
                     /locus_tag="PK049_08495"
     CDS             <13433..13813
                     /locus_tag="PK049_08495"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOK61637.1"
                     /translation="LNQRSIVVDYNHSIAGEEQVEGWDCWRITLSPKPDAPVVWGKMI
                     LWVTKKHYLILKSEYYDEEQLLIKTELARSIKEIDGRIIPTVYELIPAEESGNRTIVE
                     LNAIKFNIDINDSFFSLRNLTQVK"
     gene            13839..15254
                     /locus_tag="PK049_08500"
     CDS             13839..15254
                     /locus_tag="PK049_08500"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOK61638.1"
                     /translation="MSYFKMAQRNLWRNKRRTAITISSIFFAVLFAVVMRSFQLGSYS
                     LVIRNAIEQYYGYIRVQHPDFADDPSLENTFEFDQSLPETILRVPNVKAVTPRLETFA
                     LASSGNITKGVLVLGINPEAEKQFSNPEQFIVRYTIPKMLADSSGMEPTLTDEVKRIL
                     GAHAGESFPDSAKLARIFDDQPVAAAQLNQILNLSRVLGRFITSNDSGVVISSRLANF
                     LNIQVGDSLVMLSQGYQGASASGVYPVIGIVRIPNPELDNRMVYMPLQLAQSFSNAHN
                     LLSYMAVNLNDAADDALKVTTDSLRSTLAYTGLDIKTWQDLNRILVQQIESDNQSGKA
                     FLFLLYFVVFFGIFGTVIMMVHERMHEFGVLVAVGMQKIKLVTTVLLEMLIMALIGVA
                     LGYLVGLPVIHYYHVHPIRLTGEMAKMMMDMGFEPLMPMAPFDSYVLWQGVSVIFMVL
                     LACIYPARKILKLNTVTAIKR"
     gene            15284..>16092
                     /locus_tag="PK049_08505"
     CDS             15284..>16092
                     /locus_tag="PK049_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61639.1"
                     /translation="MLVSLSWRNVWRNPTRSMVVVVAVALGLWGGVTAMALINGWVKQ
                     RIADSIQNEISHIKIHNPDFLLNEEVDKTFSFDSVAATLDSTKGIVGFSGRLKVFAMA
                     QTDRANSGLVVLGVRPSSERSVSGIPNNLIMGSFLEGDFKTPSIVLGKKAAEELKLVN
                     YELTDSIAQKLSAMEPRLGEQIQTLVGRRYRTRSNLINALKAELEPALFNHYSETITD
                     SCASFRLNGPVVLTFQTPYGELHSAMFRVRGIYRTSNTAFDARMAYVNFDNL"
BASE COUNT         4144 a         3398 c         3565 g         4785 t
ORIGIN      
        1 tggggaaaac cgaggcagca tcgcgtatag ttgcaataac cgatcaatct aagggcgccc
       61 tgcgcaagtt ggccgattcc gagggctatc gcacttttgt aattcccgac gatgtaggtg
      121 gacgttactc ggtgctaacc cctgtaggac ttttacccat agccatagcc ggttttgata
      181 tcaggcagtt ggttgagggt gcccgtcacg ccatgaacga tacctcggcc aatgtaccat
      241 ttgagcagaa tcctgcctgt cagtacgcag cagcacgcaa cgtgctttat gccaacggca
      301 aaaaggtgga aatcatggta aactacactc ccaaacttca ttttgtaacc gagtggtgga
      361 aacagctgta cggcgaaagc gagggtaagg agaataaggg tattttccct gccggggtcg
      421 attttaccac cgatttgcac tccatgggac agtacattca ggaaggtgag cgttttattt
      481 tcgaaacggt tctatcggtc gagaagccat ggcataagtt aactattccc aacgatcccg
      541 ataaccttga ccagctaaac ttccttactg gtaaacgcct caccgaggta aatcatatgg
      601 ccgaactggg taccattgtc gcccacaacg acgggggagt gccatgcatt cagattacca
      661 ttcctgagct aagcgcatac tggattggat atttgcttta cttctttgag cgggcatgcg
      721 ccattagcgg ctacaccttg gatgttaatc cattcgacca gccgggtgtg gaagcttaca
      781 agaagaacat gtttgccttg ttaggtaaac cgggttttga ggatgagggg aaagctttgc
      841 ggaaacgctt aggaatatag ttacaaagca gtatttgtag attaaaaaga ggtgctccca
      901 agcacctctt ttttaattta aaatgattag aaagcaaatc cgattgagaa ttcagtacga
      961 atacggaaac cttcaaattt tgccacatca aaattttcgc ccgtagcacc ttcttcggtt
     1021 tcgataccgc ctttactgta aaggccacca atgaaaagat ccatggtaaa tcccgagtta
     1081 gtaacccatt gacggccaat ggctaaaacc gccctcgtaa tttccatagg ttcctttgtc
     1141 gttggtttca atgttttcaa gtttaaaatt ctgatacctg aagtatggag caaggtagaa
     1201 accatctacg gcattgcttt tgggataaaa acgtacctca ggggttaggg taagtccaga
     1261 gaattttgaa tcctcaattt ttagctaagg tagccaactt aaaatgttca ccttaaaaac
     1321 attgggttcc tctgaaacgg caggtttttc ctgtgcgaac atgggtaatg ttaacattac
     1381 caggatgcaa gtgtaattag tttttcattt ttaaaatttg gattagttta cacgaccaat
     1441 ttaataagca ttatgccaga ttactatttt ttattgaact tgatagtttt tgtactaata
     1501 acttgtaagc tggcagggtt aatcatactt attttaacta tatagatccc ctttgacaaa
     1561 cccgataggt taacagacat aacatttttg ccagcactca agttgctaat agattgggag
     1621 atgattacct gtccaagcat attaaccact tcgatgtttg cattaacaga gttttggtta
     1681 ttaaaaaatt taatataccc atcgttttca gcagggttgg ggtagaaaga tatggcgttt
     1741 tcctctgctg aattaactgg aacatcggta gttttatcag agactatggc tcgaattaaa
     1801 aagttgtaat ccagattaaa ctgagctagt tgtacccatt ggcctccgtt taggaaccaa
     1861 cctcctttgt cgggaaccat agggcctcta tcggtggcga agggataacc accggaggcg
     1921 gttgcagtgt agcccaccca gtactcctta ttggccttta tttcaactgg tgtagtaaca
     1981 ttatgggtag tccattcatc aaccttaacc tcagctgaag cgccctggga gtataccaag
     2041 gtgccagggt ttcctactgt tccaccttcc caaatcttaa ccacttcacc tgaggtaagc
     2101 ctttgtccat tatcatttcc ccttaggaag aaatcaacct ttttgatgta cttcccaaca
     2161 tagttggcca tatcgcttgc tgtaaagcgt gtggcaacag ttagggtaga agatacatta
     2221 ccaatggccg aaccataccc tctatcgttc tttagcacaa tccagggcga gaaatcgatg
     2281 gttgcattga gtgaaatggt agagcctgcg ctagtaacgt ttgtaatgtt tattccccca
     2341 ttcgatccgt cggaaaggaa actgtgcggg ttggtttggt tgttcatggt ggtacgacca
     2401 taatcggcat taaatgtggc actatttatt gttccgttca atgttagaga gccgtttggt
     2461 cggaatacat atacttcgtc gggtgggcca tcggcatttc ccataccctc gaactttgaa
     2521 ttgatacggt aaaccaacat gccatcgttt ggcaggctgg catcgtaggc ctcgcgcttg
     2581 cggtactcaa ccagaaaata ttcagtatga gtagcggggg agggaattat aaagctattg
     2641 ttagtggccg atgttgaaag cgggtttagc gtgtaggtac ctgtttgatt aattacagga
     2701 atgttatcaa tccagccacc gtaaagatat ttcatgtaag ctcccatgtt ctgtggctca
     2761 tccttggtac cttccattat atcccaaact ccaaccggtg tacgatttaa attatcatcg
     2821 tagtggtaaa ggtcaggggc tccaagggca tgaaacattt cgtggcaaag tgtaccaagg
     2881 tcgattcgag cgttgttaaa gagctgcagc tggaaggtgt agtcacccac ttgcttttga
     2941 tttattctaa cagctttact ccacagactc cattggtgtg gccaaagcag ctcgttccag
     3001 ccatcgttat ccccagcaac aataaaggaa accacatcaa catacccatc ttcatttatg
     3061 tctaagttga tgttttgggg aatctgcgaa gcaactgcct caatggcatt ttttaataag
     3121 gtgtgttcac ggtaggtttg gctcttagag cttgaatagt tatcatcatt gggatcatag
     3181 ccaatgggat ttgttgttgc attgtaaggg cggtagtagt ttcggttttg agaatcctgg
     3241 taccacacca catagttgtt tgtggttgtt gggtaaaaag tggtgcttat tgtgaatttt
     3301 ccgtaggaaa cactgttgta gtagtcggaa actgacattg atgccccatt atagatgttc
     3361 tggtagtaat caatgggctc atcgtcagga ttactgtcgg caaagcgaat gaaaataacg
     3421 atgttattca taacggttgc ctcctttgta tcctttgccc tcaatggtgc aattccatcg
     3481 gctcctttgg ttggggttag cgataggtag tgattgttga cccttttacg cttgcgcttt
     3541 gcaatgtact cctcgcgcga gatgttggta aatggctcta aaccttttgt tgctgttggt
     3601 tttactttcc caacaacaaa atcgctggct acaaattttc cgttctcttt agcggcataa
     3661 acatagtagc ccgttttagg atgctgaatg atagtgtatc catcggcatc gtgtagccag
     3721 ttgtggaatt catcaccact ggcaaaacat tcaatttttg tcccatcggg ttggtaaact
     3781 accatgggaa catacttttt gggcacccca aaaagcaacg agtacgaagt taaaagcagc
     3841 aaccatacgg ttactaaacc caaataaatg gctcttttct gcataaagtt gatgttactg
     3901 gttactaata cctgagttat tgtttacttg ttttttacaa atcctgtaaa tgttaccttc
     3961 caagttgtgg tggtttgaag caccgtggcg ttgggtaatg agtcaatctt ggtttcggta
     4021 atcaagtatt cttgattagg tttcaataca aactgtttac ctttaaaata acagtttacc
     4081 ttgtagttgg ttgcatttgt aatggtaaac tcgttatagc taaaagggta ttcattgacg
     4141 ccaattattg tgctcgaaac tccttggcca gcagtaccct taagtacttt cccaacccca
     4201 agataaagtt tgatgcttag tgagtcggta atgttgttgc ggtatatatc caatttttga
     4261 gttttggggt cgtaggagta cccagggcca tcaattcttc ttccctggta tggtactccg
     4321 ctcagggcct taccttcggt aattgagtag aactcaacaa agggaacaag gtcattcttt
     4381 ccctgctcct tttccatttt ggtgttcgaa gcacaggata aaagcaaggt gaaaaccaca
     4441 cccgatgtaa tgattcgatt catattaaac tatattgact gcaaagaaaa tattatttat
     4501 tgggttactc atttaataaa ataaagaaaa ggacgaacag caagttttgt tcgtctaacg
     4561 gcaagaaaga aatgctatac tacactacga ttttttttcc tgaacaatgc ccaatcgtgt
     4621 tgcggttcgc tccggaacgt agataaccaa tcgttggttt tccctaataa gatctctatg
     4681 caggttattc cagcccttga gctgcctaac cgagacattg tagcgttggg ctattgagcc
     4741 caacacatca cccttcttca ccctgtaaac aatacgaatt gagcctgggg gaacctcgtg
     4801 ctgatatgat gcagtatatt tagatgggtt cgccaaaatt ttttggttga aaaaaatact
     4861 gtccttgtag gcgtaaattt ctttttccct gtcaaggaag gggccaacca tttctttggg
     4921 taacctcaat acaaacgatt ttcccttagc tggaattata tcgattttgt actgtggatt
     4981 caaatcgcgg agtagctcaa tggggtaatc gagcacctct gcaatctgtt gcaggtgcag
     5041 catgttgttt acaattattg tatcggtagc agggggtagg gtaatgggtt tgggttgaag
     5101 gttatgttcc ttgtaaaagt agtaggtgta gttggcagcg atgaaagcgg gaacatatcc
     5161 gcgggtttcg cggggcaagt ggtaatagat atcccagtaa ttccttttgc cgcctgaacg
     5221 tcggatggct ttgtttacat ttccggggcc gcaattataa gccgcaatca caagtgtcca
     5281 gtctccgtaa attgagtaaa ggtctttcag aaatcgacat gctgcatggg tggcagccag
     5341 cggatcacga cgctcatcga tgaaggagtt aatggtaagg ttgtacatcc tgcctgttcc
     5401 gtacatgaac tgccaaagtc cggtagcacc agctcttgaa accgcccggg ggtttaatgc
     5461 cgattctatt accggtaaaa accgaagttc ctgaggtagt tgatacatgt cgagtatttc
     5521 ctcaatcatg gggaagtaat aatcggttag cccaagcatt acctcaacct tgtcgcgctt
     5581 tttctcagtg taggctttta tgaagctcct aacaatatta ttgtagggta aatcaattaa
     5641 tgagttgatg gtttgcagac gctttatata aaatgagtcg gggagttcgg gcccttcaat
     5701 ggtgtcgccc tcaatatcgg ctactaggtt attatcttca ttgggttgct ggatatacca
     5761 ctggtaaagc aggctatcca tctggtttat ttgtctttgc tcataaatgg agtcggaatt
     5821 taaactctgg ttctcaacct gagcaaatac tggagtgtta aaagttagga ctaagcacaa
     5881 tccacccaaa atgtatcgca tcgccttcat tctgtttcaa attattagca aacataagca
     5941 ttttaaagaa aaaatcaaga ggaatggcct aaaagtttta gtaaaactat ctctattgca
     6001 tccgaaaatg ctaatatgcc tatctactca agtgctgata ttattaattt cgccaaatcg
     6061 attggtttgg tattatcggc atcgacaatg atttgagctt tctcgtaaaa tttttttcgt
     6121 tcgtcaagaa cacgtataat gtaatcgttt agttcctcgc gagttttacc ccaaagtaaa
     6181 ggcctatcga tttttgattc caccaaacgc gaaaccaggg ttgaaacctc aagcctaagg
     6241 taaacagtaa tgccatttcg gttcatgtag tcgatattgt tgtaaaagca cgatgttcca
     6301 ccacccgatg aaatcacaaa atcgccgtta aatgaacaaa cctctttgag tgcttcgttc
     6361 tcaataagcc ggaagtgctc ttcgcccttt gtttcaaaaa tttgtttaat tgttctattg
     6421 tgcttggttt caatatactc atccaaatcg aggaattggt aacctaggaa atgagccagt
     6481 tcccttccaa ctgtggtttt tccgctcgac atgaatccta tcaggaaaat cttcattgtt
     6541 taatgcttta tttaagttta atgcaaaatg tctaattcac aatattttat aaaaaaaatt
     6601 aagggagatc aaagatataa aaaaagagca gaaaaactgc tcttttttgc taaagttcca
     6661 agctcatttg ctcagctgaa gaacctgaat cgttatcttc cgattctgaa tcaggaattc
     6721 cgcttgtttc ttcctcaaaa gctgatcttt ccttttccag gggttcaata aactcaaccc
     6781 gttttacatc gaacgtggtt agacgtttgc cttttgcctt gtagcccttt acggcaataa
     6841 aggcatcaac atcgatattc tcgggttcgc gatttgcgtt tttgcctcca aaaacaagtt
     6901 tcaaagccgg gaatacgtcg gcattcaaag ctataagttt tgagctatac tcgtcgggga
     6961 taaaggattg ggttttctcg gtgggttcaa aacggaaacg tttaaggtag tactgttttt
     7021 gctcgccatc gaaataaacg gccgagaata ccttgtgggg gttaaacttt tcaaccagta
     7081 ggggttgcac atcgaacctg agggcataat tggtgttggc aaggaaatag gtaccatcgg
     7141 ggttcacaac cagaacgcgt tcatcgggca agaattcgcc aagcagttca ccttgtccct
     7201 cgtggttcag gcgcataacc tcaggatcgt accaaacctt tacaccctcc actgcgccag
     7261 caatcttttc cttgagcatg attttgtgga tggcgtatcg ggtaaagata tttccctggg
     7321 agccacgtcc tttaactgcc agttggctga aatccagttc aagtataagg ttacggaggc
     7381 gtggccttgg tttcaggtaa accttcagta cttcggtttc accatttggg tttgccgaaa
     7441 gccagagtat ttgtgaaccc tgtgtcccct ttgttatgtc gtactcctta tcgcgtgtgg
     7501 ttccgttaat ggcgcatcgc ttcatcatga tatcgccgtt taggccatcg cggtaaacca
     7561 cattgtaaat tgttcgggta tcgttcttct tataaactcc aacgtggaca atatccttac
     7621 caaagaagtc cttttcctga accttacgga tgtagtactt gccattgcgg agaattacta
     7681 ttacatcgtc gatatcggag cagtcgcaaa cgtattcgtc ttttttcaga cctgtaccca
     7741 caaagccttc ggctcggtta acgtaaagtt tttcgtttgc cacaaccacc ttagtggcaa
     7801 caatggtatc gaagctgcgg agttcggttt tgcgatctct tcctttcccg tacttctttt
     7861 tgatttgctg gtagtaatcg atggtataag ttactatatt ggcaaggtga tgcttaactt
     7921 gctccatttc ggcttccacg gccttgatat gctcatcggc ctttttgata tcgaatttcg
     7981 atatcttgcg cacgggcttc tcggttagct tcagaatatc gtcgcgggta atctcgcgcc
     8041 ggagtagttt gcggtaaggg tcaaaacccc tctcaatggc ttctattacc aaatcccatg
     8101 tttctgtatc cttttcaagc tcacgataaa tgcgctcttc aaagaatatc ttttccagcg
     8161 acgagaaatg ccaatcctcc tccagctctg ccagcctaat gttaagttcc atggtaagga
     8221 gttccttagt tctatccacc gaagccctaa ggatatcctt aacgcccatg aaacggggct
     8281 tatcgtcgta aatgacgcat gagttggttg atattgacat ctcgcagtcg gtaaaggcgt
     8341 aaagggcatc aattgtcata tcggggttgg cgtcggggtg caggtggatg agaatttcta
     8401 cattctcagc ggtattatcg tcaatccttt tgattttaat cttccctttg tcgttcgctt
     8461 taaggattga ttcgataagc gatgtggtgg ttttcccaaa gggaatctcg gttatggcaa
     8521 gggttttacg gtcgagtttt tggatgcggg cgcgtacctt aaccgctccg ccgcgtaaac
     8581 catcgttgta acggctacaa tcggctaagc cacctgtggg gaaatcgggg taaagttcaa
     8641 aatccttacc ctttaggtag ctgattgccg catcaattag ctcattaaag ttgtggggta
     8701 aaatttttga ggctaaacca accgcaatac cttccactcc ttgcgctagc aaaagcggga
     8761 acttaaccgg aagcgttaca ggctcctggt tacggccgtc gtagctgagc atccactcag
     8821 tggttttggg gttgaaaacc acctcaaggg cgaattttga tagcctagcc tcaatgtaac
     8881 ggggtgcggc tgcactgtcg cctgtaagta tgttgcccca gttaccctgg gtgtcaatca
     8941 acaaatcttt ttggcctagc tgaaccaagg cttcgccaat tgatgcgtcg ccgtggggat
     9001 ggtactgcat ggtgtaaccg ataatattgg ccaccttatt gtatcgccca tcgtcgagcc
     9061 gtttcatggc gtgcagtata cggcgctgca cgggctttaa cccgtcatcg aggtgtggaa
     9121 cggcgcgttc cagtataaca tacgaggcat agtccaaaaa ccagtccttg tacatcccgg
     9181 tgatgtgggt ctttttggcg gcctcgccgg taatcctttc tattttggca tgaagttctg
     9241 gcgaaacagg tttcccctcg ccttcgctta aaagctcatc cgcttcaaaa tcgtccaatc
     9301 gcattttctt gagctattgt aggttgatgt ttatgctacc aggtttttat tctcaacgtc
     9361 atggttgcct tcctcgtcga tgttattctc atcggctttt ggggctggtt cctcatcaat
     9421 aatatcctct tcaattctga gattattgat aatgaaatcc tgacggtcgg gcgtgttctt
     9481 gcccatgtag aactccaaaa ggtcatgtat ttggtcttcc ttggtcaaac gaaccgggtc
     9541 gagcctcatg tcggggccaa tgaagttctt aaactcatcg ggcgaaattt cacctaaccc
     9601 tttaaacctt gttatttcag ggtttgggcc aagttcggct attgcctttt ccttctcctc
     9661 gggggtatag cagtaaacgg tcttcttctt attacgaacc ctgaaaagtg gggtttgaag
     9721 gatgaagagg tgctcctggc ggattagctc agggaagaac tgtaggaaaa aggttatgag
     9781 cagcagcctg atgtgcatac cgtccacatc ggcatcggtg gctattacaa ttttgttgta
     9841 gcggaggttt tccatgtcct cctcaatgtc cagcgcagct tgtagcaggt taaactcctc
     9901 gttctcgtaa actatctttt tggttaaccc gtaggtgttt aacggtttac cgcggagcga
     9961 gaatactgcc tgtgtattta catcgcgcga tttggtgatt gaaccgcttg cggagtcgcc
    10021 ctctgtaata aaaagggtac tttcatgcgc acggtcatcc ttgctgttgt aatggatcct
    10081 gcaatcgcgt agcttgcggt tgtgcaggct tgccttttta gcccgctcac gggctatctt
    10141 tttgataccc gatatagctt tacgttcctt ttcggactca ataatctttt gtagcagaat
    10201 ttgcgcagtt tcgctattgc gatgcaggaa attatcgagt ttttccttaa taaaatcggt
    10261 gatgaattgc tttaccgagg gtccgttagg gcccatgtcc ttgctgccaa gcttggtttt
    10321 ggtttgtgac tcaaatacgg gatcttcaac cttaatgctt atggctgcta taatggaagt
    10381 acgaatatcc gatgggtcaa agtccttttt atagaactcc cggatggttt taacgtaggc
    10441 ttcgcggaat gcggttaggt gtgtaccgcc ctgagtggta tgctggccgt tcacaaaggt
    10501 atagtagtct tcgccgtagc caacaccatg ggtaattgcc acctcaatat cttcaccttt
    10561 caggtgaata atggggtaga gtggctctgt tgttatgttt tcgttcagta ggtcgagaag
    10621 cccgtttttt gagataaact ccttgccgtt aaagctgatg gttaggttgg tgttgaggta
    10681 ggtgtagttt cggatcatgg tttcgatata ctcatcctgg aatgagtatt gaccgaatac
    10741 cgcctcgtca ggagtaaact gaacgtaagt accgtttgcc tcgccattgg cggggtattg
    10801 cttatcttta acaaggatgc cgccggcaaa ctcggcctcg cgaactaaac cctcgcgaat
    10861 actctttatg ataaactggt tggaaagcgc attaaccgcc tttataccaa ccccatttaa
    10921 gcctaccgat ttcttgaaaa ccttggagtc atacttggca cctgtattca ttttggaggc
    10981 aacgtctatg agtttccctt gcgggattcc acggccatag tcgcgtatcg aaacggtttt
    11041 atcgttaacc aaaatgtcga tatgtttccc attccccatc atgaactcat caatggagtt
    11101 gtcaaccacc tccttaagca gaatgtaaat gccatcgtcg ggctgagagc cgtcgccaag
    11161 cttaccgata tacatacccg ggcgcttgcg aatgtgctcc tgccattcca acgttttaat
    11221 actatcttca gaatatccaa cactcatgac tacttctcca gtttgctgca aaaataacca
    11281 aattgaattg gcatgaactt tttttaccca aataaatatc aacaggtttg ggcgacttgt
    11341 aaaccgattg gaatttaatg agttattgta ataatttggc ttgcggtttt aaatcattaa
    11401 accaaactcg ttgtttccga aacatgggag aattagctat gcgttaacgg caatgtttgg
    11461 gtattgtttc ttgtaaatat ctgtgtggat gtgtaaaaaa agagagggct acggcatttg
    11521 agggtgtagc cccttccttc taatcaacta cattttcttc ccaatgtaaa aaacatagcc
    11581 atagtaatcc ttgtacctag cgtagagctg agcctcgtgt agtaggtttt caactaatcg
    11641 catggcattc tgattacctg catacttttc catgaaaagt ttctgtgctg ttacttgtgg
    11701 agcataaaaa tgctcagtcc aacagttttc gggcagtatg aaagtagcaa ccggaatata
    11761 accagccttt tgcatgattg ccaccttgct tggaatggtg tcaatttccg ggtaagcatc
    11821 taaccagaac tgctcaattt ctgcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11941 nnnnttggaa tggtgtcaat ttccgggtaa gcatctaacc agaactgctc aatttctgct
    12001 ggacgctctg gcgtaaacca cgatgcttca gttactgcca cgtagccccc tttcttgagg
    12061 aatcgccgcc attcggttag gccgcgctca aaaccaatgt tatagatggc tccctctgac
    12121 catataaggt ctagctcttc gacctgaaag ggcaggttgt ccattgatcc aactattgct
    12181 ttggctttat ccgttaggcc tagcttggta gcattctggt tgaaaatatc aacaaattgt
    12241 gggaacaggt caatacctat tattgttccc ggtgtgtttt gggcaagcac catggtttgc
    12301 ccccctgttc cgcatcctat atcggcaatt ttggcgtttt gggtaagatt gtcgataaaa
    12361 ctcaatgcct tgattgttac ctcagggctg cctggccctt gacgttcgac atacgaaaaa
    12421 tactcacata ttaatgctat atcgaaatcg tgtatggagt tgttttcttg ttccatgatc
    12481 tagtagttat tttttaacag gcaaaagcat ggctttgccg ccattaaatt gtttgaaaat
    12541 ttataaaaga tattacatgg caagttgctg ccaaacagaa atggttgtgt aatcttgtga
    12601 tttactcaag attacctaac ctaccagctc cgaattcgat aaatacatcc aaaagtttta
    12661 gacctttcaa aggtaagcat atttttttga gtttcaattt tgttttacaa caaattatgt
    12721 atcagttaag tttagggtag attgaataga tttttttatt gataatctcg atttttcgta
    12781 gtgtttatca attcaggaat caaacgtaaa aattttcttc caaaaaaatt gaatggtaaa
    12841 aaacaatttt ttatatttga ccgaccgttc aaccaaaaat aaatatgcca cgcacaaagc
    12901 agcaagttga agcaatccgc cagcagcgag ttatgcaaat ttttgacgct gcacttgatg
    12961 tttttgccca caagggtttt cattcggcaa ccattgccga catcgcccaa aaggcaggaa
    13021 ttgcaaaagg gttgctttac cattactata gctccaagga ggagctgctc tcccatatcg
    13081 tgattaatgg aatgaacaat ttgatagaaa agtttgatga aaaccacgat ggggtactaa
    13141 ccgatgcaga gatggaactg tttattaacg atatgttccg taagattgtc gaaaaccgca
    13201 actactggaa gttgtacttc tccgttatgc ttcaacccaa cgttttcagc aaggagttga
    13261 tgcaaaaact tgagggtatc tatgaaccaa tggtatcaat cttaattcag tactttaggc
    13321 gcaagggcta tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnctcaacca
    13441 gcggtcaatt gtagttgact acaaccactc cattgccggt gaggaacaag ttgaggggtg
    13501 ggattgttgg cgaattaccc tttctcccaa gcccgatgct ccggttgttt ggggcaaaat
    13561 gattctttgg gttactaaga aacattacct tatcctaaaa tcggaatact atgatgaaga
    13621 gcaactgctc ataaaaacgg agttggcacg cagcattaag gagattgacg gaagaataat
    13681 acctaccgta tatgagctga ttccggccga ggagtccggt aacagaacca ttgttgaact
    13741 gaatgccatt aaattcaata tcgatattaa cgatagcttt ttctcgctac gtaatcttac
    13801 ccaggttaag taggcgaaaa ccaataaaac attttggtat gagttatttc aaaatggcac
    13861 aacggaacct ttggcgcaat aagcgccgaa cggccattac catctcaagt atcttttttg
    13921 cggtgctatt tgcggtagta atgcgttcgt ttcagctggg ttcatactcg ctcgtgatac
    13981 gcaatgccat tgagcagtac tacggctata tccgtgtcca acaccccgat tttgccgatg
    14041 atccctcgct ggagaacacc tttgagtttg accaaagtct tccggaaaca attttaaggg
    14101 ttccaaatgt aaaggcagta accccacggc tcgagacttt tgccctggct tcgtcgggta
    14161 acatcaccaa gggcgtgctt gtgcttggga taaaccccga ggccgaaaaa caattctcaa
    14221 accctgaaca gttcattgtt aggtatacca ttcctaagat gctggccgat agttcaggta
    14281 tggaacccac tcttactgat gaagtaaaaa ggatactggg agcccacgcc ggcgaatcgt
    14341 ttcccgattc cgcaaagttg gccagaattt tcgacgacca accggttgct gctgcccagc
    14401 taaaccaaat cctgaacctg tcgcgggtgc tggggcgatt tattaccagc aacgatagcg
    14461 gtgttgtaat ctcatcgagg ctggccaatt tcttaaacat tcaggttggc gattcacttg
    14521 tgatgctgag ccagggttac cagggtgctt ctgcatccgg ggtttatccg gttattggca
    14581 ttgttcgaat acccaaccca gaactcgaca accgaatggt ttacatgccg ctacagttgg
    14641 cgcaaagttt ctccaatgca cataacctat tgtcgtatat ggctgttaac ttaaacgatg
    14701 cagccgacga cgcactaaaa gttaccaccg atagcttaag aagtactttg gcgtatactg
    14761 gcttagatat taaaacctgg caagacctga ataggattct ggttcaacag attgaaagcg
    14821 ataaccagag cgggaaggct ttcctttttc tgctctattt tgttgtgttc ttcgggattt
    14881 ttggaactgt aataatgatg gttcacgaac ggatgcatga gtttggtgtg cttgtggctg
    14941 ttggtatgca aaaaattaaa ctggtcacaa cggttttgct cgagatgctt attatggcat
    15001 tgattggtgt tgctcttggc tatcttgttg ggctaccagt aattcattac taccatgttc
    15061 atcccattag gcttaccggt gaaatggcaa aaatgatgat ggatatgggt tttgaaccct
    15121 taatgccaat ggctccgttc gattcatatg tgctctggca gggcgtttca gtaattttca
    15181 tggttttgct ggcttgcatc tatcctgccc ggaaaatact aaagcttaac actgttacag
    15241 caattaaacg gtaatcaatt gagttgaaaa aaaatttacg aacatgttag tttcacttag
    15301 ctggcgaaat gtttggcgca atccaacccg gagtatggtt gtggtggtag ctgttgcact
    15361 tggactttgg ggtggggtta ctgccatggc ccttataaac gggtgggtta aacagcgcat
    15421 agccgatagc attcagaacg agatatcgca cattaaaata cacaaccccg attttttact
    15481 caacgaggag gttgataaaa ctttcagttt cgactcagtg gccgctaccc tcgattctac
    15541 aaaggggatt gttgggttta gcggaaggtt gaaggttttt gccatggctc aaaccgatag
    15601 ggccaactcg ggcctggttg ttttaggtgt taggccaagc agcgagcgga gtgttagtgg
    15661 tattcccaac aatttgataa tgggcagttt tcttgaaggt gattttaaaa ccccttcgat
    15721 tgttcttggg aagaaagcag ccgaggaact taaattggta aactatgagc taaccgatag
    15781 cattgctcaa aaactctctg caatggagcc aaggcttggg gagcaaatcc aaacacttgt
    15841 ggggaggagg tataggactc gctcaaacct tattaatgct cttaaagcag aacttgagcc
    15901 cgcactgttc aaccactact ccgaaaccat taccgactcc tgtgcttcgt tcaggttgaa
    15961 cggtcctgtg gttctaacat ttcaaacacc ctatggtgag ctgcatagcg cgatgttcag
    16021 ggttcgaggt atttaccgca cctccaatac agcctttgat gcacgcatgg cttacgtaaa
    16081 tttcgataat ct
//
LOCUS       DAOSAL010000059        15907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_2529_length_15907_cov_50.967007, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000059 DAOSAL010000000
VERSION     DAOSAL010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 15907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15907
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2529_length_15907_cov_50.967007"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..2936)
                     /locus_tag="PK049_08510"
     CDS             complement(147..2936)
                     /locus_tag="PK049_08510"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HOK61640.1"
                     /translation="MKKILSITLKVLAALVTLFIAAAIVLPIIFKPQLMQVAKKEANS
                     MLNAKVDFTDFQVSLIKGFPNLYVGMKGLTVVGIDSFANDTLVAFKEFGVKVNLWSVI
                     DMTDIEVKSILLDEARVNAHVLPDGRVNWDIMKPSETPETEMEDTAAATTVSTKVSLK
                     RFEIRNANIAYIDDSSNMKATIQNFNLTLSGNMGMSQTELKLKTSIESLNFWMDGIRY
                     LKNSAVGFDAIIAANLDSSYYAFKENAFSINQLKLLFDGSVRMPGNDIDINLNFKTPS
                     TDFKALLSMVPAVYMTDFEGLQASGKFGIEGYVRGTYNDSQMPSAFVAINVDNGMFKY
                     PDLPKSLTDVNISTKIWFDGVNMDNTSVNVDRFALNLGGNPFSMRMRVATPISDMQVD
                     GNITGKIDFATLSDVIPMDSTYMEGLLESNLDFGGKLSFIEKEQYERFKADGLLKLSR
                     FKFKSPDIPAEVKLNEVTLNFTPQYVNLANFDMSIASSDIRMNGRLENFIPYVFKDET
                     LSGNLNVNSNLLDVNELLTGESAPEEEVTDTTALSVIELPRNINFSTRVDMKKIVYDK
                     LNIENLAGNVALSGGVADLKNLSMNMLKGDVRMSGTYDPRDVKAPMVNFDFDMRNIDI
                     PTAFKSFTTLQKIAPTVKDMTGNVSTQFKFTSMLDTTMMPVLNSINAYGKLQSKSIGV
                     SNSKAFAKLADFLKKDEFRNPSLKDVNVSFSIKNGRIYIEPFDTKIAEAKMNFGGDMG
                     IDQSLNFRAKVSVPSSYLGGVANLANDLLGKYGAKLPQTIDVNLKILGTSSKPDVQLD
                     SGGGSAESSSSVKETAKEAVKEKVDEAKVEARQKAKAEADKLIADAEREAEQIRAEAA
                     KRAEQIRAEAEAQAKKTEAEGQKKGPIAERAAKEAAKKIRQNGEAAAQKVIAEADEKA
                     NAVVTKAKAEAAKLE"
     gene            3129..3344
                     /locus_tag="PK049_08515"
     CDS             3129..3344
                     /locus_tag="PK049_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4492 domain-containing protein"
                     /protein_id="HOK61641.1"
                     /translation="MNLIKRIFRFYYEGFKSMTIGKTLWLIILIKLFVMFAVLKVFFF
                     PNILKRNFKTDEERSKYVIEQLTTKPK"
     gene            3353..4915
                     /locus_tag="PK049_08520"
     CDS             3353..4915
                     /locus_tag="PK049_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HOK61642.1"
                     /translation="MEHFDLSLVNWSRAQFALTAMYHWVFVPLTLGLAFIMAFMETLY
                     YKTGDEEWKRITKFWMTLFGINFAIGVATGIILEFEFGTNWSNYSWFVGDIFGAPLAI
                     EGIMAFFMESTFIAVMFFGWNKVSKKFHLLSTWLVALGANLSALWILVANAWMQYPAG
                     MFFNPDTARNEMHNFWEVLLSPVSVNKFLHTISSGYVLAALFVVGISAWYLLKGRNVL
                     LAKRSIIVASVFGLITSVFLALTGDGSAYQVAQKQPMKLAAMEGLYEGKEGAPLIVIG
                     ALTPGKQYNDNKDPYLFRIQIPKLLSFLGYRSGDAFVPGVKDIVEGGYTYTDKDGNQK
                     VALSAEQKIERGRIAIQALADYKNAKELGDTTAMAYHRKLLDANFEYFGYGYLNDPKS
                     IIPNVPLTFYSFHIMVGLGFLFIAFFILILYLVFFKEFKINKWLLRTAILMIPLAYIA
                     SQAGWIVAEVGRQPWVIQDILPTVAAVSRIDASAVQVTFFLFFIIFTTLLIAELRIMF
                     TQIKKGPKDGGK"
     gene            4918..6069
                     /locus_tag="PK049_08525"
     CDS             4918..6069
                     /locus_tag="PK049_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HOK61643.1"
                     /translation="MVDTTYIFLQHYWWFIISLLGALLVFLLFVQGGQTLIYTLGKTD
                     MERTMIVNALGRKWEFTFTTLVTFGGAFFASFPLFYSTSFGGAYWVWMAILFCFVVQA
                     VSYEYRSKPNNFLGPKTFEVFLFINGLFGTVLLGTAVGTFFTGSQFSVAKINLTNISN
                     PVISSWETPWHGLEAALNLHNVALGLAVFFLARVLAILYFMNHINHPEIFARSRKHLL
                     YNAIPFLVFFLTFVIWLFIREGFAVNPTTGDVFMQKHKYLLNLIQMPVVLVLFLGGVV
                     LVLWGIAKSLAKPYTKGIWFTGIGTVLTVLSLFLIAGYNNTAYYPSTFNLQHSLTIFN
                     SSSSKFTLTVMSFVSLLIPFVAAYIWYAWKSINVKKIDEEEMKNETHVY"
     gene            6082..6204
                     /locus_tag="PK049_08530"
     CDS             6082..6204
                     /locus_tag="PK049_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61644.1"
                     /translation="MRFLNEILWFASWPVLIYLSYLLSVWAVKLWEKKNPDQVK"
     gene            6235..7236
                     /locus_tag="PK049_08535"
     CDS             6235..7236
                     /locus_tag="PK049_08535"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOK61645.1"
                     /translation="MNIYKLLKLNRAIRSNRLKLLLIFMVQLLNRRYYSVRIDPIFAC
                     NLRCLMCYFSKSRKAQPKTFSKVELELLAKNLFGNALQVMVGCGAEPTIHPDFIYLVE
                     LAKRHNVPHVGIVTNGQLLSKNDLLTLSSAGLNELTLSVHATQKETYENFMVGAKWEK
                     LIGLLENIKILRDDGKIAFSIRMNFTANPDNIQELEQFFDVFGDYPINTLQVRPIMEI
                     GGAYFKPFSCESISLYNSVIESLFTTCKKHGITLLANTQNPSSQQKSNPVIAQTYKYV
                     SPQVVVDNNFRWQTETYRQFLKRTGWHKAMLKSIFSIRLSNSSTDLAERYGGQYDVL"
     gene            7270..8397
                     /locus_tag="PK049_08540"
     CDS             7270..8397
                     /locus_tag="PK049_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004348363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase type II"
                     /protein_id="HOK61646.1"
                     /translation="MNTKTELEPRIEISPLTEWGLNLNYPLIIAGPCSAESEEQVLTA
                     AHALARNGNVRVFRAGIWKPRTRPGGFEGAGEKGLQWLKRVKDETGLAVATEVAMPQH
                     LELALKYGIDMVWVGARTTTNPFLVEQLASVLAGTSIPVLVKNPITPDLELWIGTLER
                     LWRSGITRLAAIHRGFHPYERSRLRNIPKWELAIDLKSRIPSLPIICDPSHIAGAANL
                     VPEIAQHALDLSMDGLMVEVHPNPAIALSDARQQLTPDAFTNMLSELTFHQNTSVNNE
                     FANFLEQVRNKIDSIDQQIIELLANRMRLVEQIGEYKRTNNVTIFQLRRWEKILESRI
                     ECGKRLGLDEEYIKSLLQLVHKESIKKQAEILRGNRLTNGE"
     gene            complement(8401..8658)
                     /gene="purS"
                     /locus_tag="PK049_08545"
     CDS             complement(8401..8658)
                     /gene="purS"
                     /locus_tag="PK049_08545"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF004630.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HOK61647.1"
                     /translation="MKFLAEINVMPLKALLDPQGKAVNATLHGIGFKEVSNVRIGKHI
                     NLEIEAANREVAMEKVTEICSKVLSNPVMEGYDFKLTEITE"
     gene            complement(8706..9464)
                     /gene="pssA"
                     /locus_tag="PK049_08550"
     CDS             complement(8706..9464)
                     /gene="pssA"
                     /locus_tag="PK049_08550"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HOK61648.1"
                     /translation="MNRITRVIRHIPNGITLLNLFSGSLSIVAAFEGRLELAGLLIII
                     SGVLDFLDGFTARLIGAYTQLGKELDSLSDVVSFGVAPAVILYHMLRQSIGISDCHGI
                     FEGHFVLVIPFLVAVFSSLRLGLFNIDERQTSSFIGLPTPANAFLIIGLVFGANSSWS
                     GLFNPIVGSQVVLIVLAIISSFLLVCELPMFSLKIKSLKPSVAYKQLTFLAGAVVFIA
                     IFQKAALSIIIVWYIVLSTIYYLWDKLINKDFQS"
     gene            complement(9489..10052)
                     /locus_tag="PK049_08555"
     CDS             complement(9489..10052)
                     /locus_tag="PK049_08555"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOK61649.1"
                     /translation="MIDYLVKLDTDLFLFLNGLHSPFWDSVMLFASGKLTWLPFYLLL
                     IYFIARKHKWKTLWWLLAIAVVVLVADQLSVHLFKNVFQRLRPCHNPDLSGVIHLVGR
                     CGGKFGFVSSHAANTFGVAVFLSMLYRNRWAGVGLLIWAAFVSYSRIYLGVHYPADVI
                     VGALLGSVVGFALWKPATWLMERYTKS"
     gene            complement(10055..11047)
                     /gene="obgE"
                     /locus_tag="PK049_08560"
     CDS             complement(10055..11047)
                     /gene="obgE"
                     /locus_tag="PK049_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOK61650.1"
                     /translation="MSNSNFVDYVKIFCRSGKGGAGSVHFRREKFVPKGGPDGGDGGR
                     GGHIYLKGNSQLWTLLHLKYQRHIFAGDGESGSGARCTGKDGEDVYIEVPIGTIARDA
                     ETNEVIAEITEHGQVEMILKGGRGGLGNWNFRTATLQTPRFAQPGEPAQEGYVILELK
                     ILADVGLVGFPNAGKSTLLSVVSAAKPKIADYPFTTLVPNLGIVRYRDDRSFVMADIP
                     GIIEGAHEGKGLGLRFLRHIERNSMLLFMVPADSDDIYGQYQILVNELKMYNPELLDK
                     KRALAITKCDLVDEQAKKKISKHLPDIPAVFISSVTGEGIERLKDVLWDNLNQQ"
     gene            complement(11308..11703)
                     /locus_tag="PK049_08565"
     CDS             complement(11308..11703)
                     /locus_tag="PK049_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61651.1"
                     /translation="MKPKLTEILLVFGLVLAGILKGITSSDKLLLVIIFLSILLSGYY
                     LFFYRNNISGDSMLLNIATRWALSLFPIAFVITFFIPNTGRILSLLYFAFCIIITILK
                     SRNKTSDRYEQIRMYLLLTILSCIGLSFP"
     gene            complement(12059..12661)
                     /locus_tag="PK049_08570"
     CDS             complement(12059..12661)
                     /locus_tag="PK049_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61652.1"
                     /translation="MNNQINLNSNSNRIPDSHEQNNSIDIDTLLIKLKNEDARNLQKM
                     KGLKRLFFTMIIIYTLFMIVNPDPDLQLHHRISGLFYVLSFTYFALLFRKYHNEFSKI
                     DYSISSLEMFKKAAERHNLTFSRYLLVLPSLIFIDIAITISEYYRWTSVEPLNRILIV
                     QAILLPIFIMSGFIGYLLWRKRQKPLRDRALQIIKELNKN"
     gene            complement(12654..13166)
                     /locus_tag="PK049_08575"
     CDS             complement(12654..13166)
                     /locus_tag="PK049_08575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOK61653.1"
                     /translation="MSREERFKRTVDENKEKIMRICRYYAPSAEDQKDMYQEILINIW
                     KSLDSFRGDSKVSTWVYRIAVNTSLSYAGKQYKLMKLNVDIENTNIKNFLSTEFNDNM
                     LKDDQFQRLQTHLNQLSVIDKAIMGLVLDELTSKEIADIIGITEPNVRIKIHRIKENL
                     RNQMKGENYE"
     gene            complement(13416..13991)
                     /locus_tag="PK049_08580"
     CDS             complement(13416..13991)
                     /locus_tag="PK049_08580"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOK61654.1"
                     /translation="MLNIVLFGPPGAGKGTQSQKIIEKYKLQHLATGDMLRAAIREQS
                     PIGVEAQKYIDKGQLVPDEMVINLIAGELDNHHDVPGFVFDGFPRTTVQAKKLDEMLD
                     AKKAPITIMLALEVEYDELLARLLNRGKMSDRSDDQDEGIIRGRLDIYNKTTLPVMDY
                     YKAQGKYRGVNGMGTIDEIFERICQVIDSSR"
     gene            complement(14334..14762)
                     /locus_tag="PK049_08585"
     CDS             complement(14334..14762)
                     /locus_tag="PK049_08585"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOK61655.1"
                     /translation="MFTIRKATLADIEPVYQLICDLEGESLNYSYFAENYRRNLEDST
                     VHYWVACHGEKVIGFLSMHVQRILHHVRLTCELQELNVLPEYRSQGVGSMLMEQAEKL
                     AKELGLEEVELTTRNYRVRTQEFYKRLGYEQTHLKFVKKL"
     gene            complement(14768..15514)
                     /gene="mtnP"
                     /locus_tag="PK049_08590"
     CDS             complement(14768..15514)
                     /gene="mtnP"
                     /locus_tag="PK049_08590"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HOK61656.1"
                     /translation="MPTIGIIGGSGLDNPDIIKDATTLRVDTPYGNPSSLLRVGTIGS
                     NRVIILARHGHSHEYSPTEVNYRANIYALKYQGADCIIATTACGSLRAEIERGDFVLL
                     DQFIDFTRKRINTYHESFASGAVHTPMADPFDSALRAHILDCAGRVGVKCHPRGTVVT
                     IEGPRFSTRAESMMFRSWGADVINMSVATEVALANELAIPYAAIAMSTDYDCWKTDEP
                     PVSWEEIVRIFEQNAQRMIALLRMVVETLK"
BASE COUNT         4467 a         3562 c         3223 g         4655 t
ORIGIN      
        1 tcggctagct cagcatgaca tgagggctaa attctcttct actaacaacc aacaacagta
       61 aactataaac taaatcatct gcgtcatttg ggctccattt caaacgctaa acaccaaata
      121 ccaaaagtaa tcaaccaaaa ttcaagctac tccagcttag ctgcttcggc cttagctttg
      181 gttacaacag cattggcttt ttcatcggct tcagcaataa ccttttgggc agcagcctcg
      241 ccgttctgcc gtatcttttt tgcagcctcc tttgctgccc gctcggcaat tgggcccttc
      301 ttttgaccct cagcctcagt ttttttggct tgagcctcag cttcagccct tatctgctct
      361 gcacgctttg ctgcttcggc ccttatttgt tctgcttccc tctcggcatc ggcaattaac
      421 ttatcggctt cagccttggc tttctgacgt gcctcaacct tggcttcatc aaccttttcc
      481 ttaacagcct ccttggctgt ttcctttacc gaagatgagc tttctgcaga accaccaccg
      541 gaatcgagct gtacatcggg tttagatgaa gttcctaaaa tcttaagatt tacatcgata
      601 gtttggggta gctttgcacc atactttccg agcaaatcgt tggcaagatt agccaccccg
      661 cctaagtacg acgagggaac cgaaactttt gccctgaagt taagggattg gtcaataccc
      721 atatcaccac caaagttcat cttggcctcg gcaattttgg tgtcgaatgg ttcaatgtaa
      781 atccttccgt tctttatact gaagctaaca ttcacatcct taaggcttgg attgcggaac
      841 tcgtcctttt tcagaaaatc agctagcttg gcaaatgcct tagagttact aaccccaatg
      901 ctttttgatt gcaacttacc gtaagcattt atgctattca aaaccggcat catagtagta
      961 tcgagcatgg aagtaaactt gaattgggtc gatacatttc ctgtcatatc cttaaccgta
     1021 ggtgcaattt tctggagcgt tgtaaacgat ttgaaagctg tgggtatatc aatgttgcgc
     1081 atgtcaaaat caaaattaac cattggggcc tttacgtccc tggggtcata ggttcctgac
     1141 atacgaacat caccctttag catattcatc gatagatttt ttaggtcggc cacaccacca
     1201 gagagcgcta cattacctgc aagattttcg atatttagct tatcgtaaac tatctttttc
     1261 atatcaaccc gggtagaaaa gttgatgtta cggggcaatt caataacgct aagggctgtg
     1321 gtatcggtaa cttcctcctc gggagcactt tcaccagtaa gcagctcatt aacatcgagc
     1381 aagttcgagt taacgttaag atttccgcta agggtttcat ccttaaaaac gtaaggaata
     1441 aaattttcca gtcggccgtt catgcgtata tcacttgaag caatggacat atcgaagttt
     1501 gcaaggttaa cgtactgggg tgtaaagttg agcgtaacct catttaattt tacctcagca
     1561 ggtatatcgg gcgacttgaa tttgaaacgc gacaatttaa gcaggccatc ggccttaaag
     1621 cgctcatact gctccttctc gataaacgag agtttcccac caaaatcgag gttcgactcc
     1681 agcaatcctt ccatgtatgt gctatccata ggaattacgt ccgaaagggt tgcaaaatca
     1741 attttaccgg tgatgttacc atcaacctgc atatcgctga ttggggttgc cacacgcatg
     1801 cgcatgctga atgggtttcc gcctaggttc aaggcaaacc tatcaacatt caccgatgta
     1861 ttatccatgt tcacgccatc gaaccaaatc tttgtgctga tatttacatc ggttaacgat
     1921 ttgggcaagt cgggatattt gaacatcccg ttatcaacgt ttatggcaac aaatgcgctt
     1981 ggcatttggc tatcgttata ggttccgcga acatacccct caatgccaaa cttaccagag
     2041 gcctgaagtc cttcaaaatc ggtcatgtac actgctggca ccattgataa aagcgccttg
     2101 aaatcggttg aaggagtttt aaagtttagg ttgatatcga tatcgttacc gggcattcga
     2161 actgacccgt cgaatagcag ttttagctga ttgatgctaa acgcattctc cttaaacgcg
     2221 taatagctgc tatcgagatt tgctgcaata atggcgtcga aaccaactgc tgaatttttc
     2281 agatatctta ttccatccat ccagaagttg agcgattcaa tactcgtctt cagcttgagc
     2341 tcggtttggc tcatgcccat gttacctgaa agggttaggt taaagttttg aatagttgcc
     2401 ttcatgttcg acgaatcgtc gatgtatgca atgttggcat tgcgaatctc aaacctttta
     2461 agtgatacct ttgtgctaac ggttgttgct gcagcagtgt cttccatctc ggtttcgggc
     2521 gtttccgatg gcttcatgat atcccagtta acacggccat cgggcaacac gtgcgcattt
     2581 acccttgcct catcgagcaa aattgatttt acctcaatat cggtcatatc aattacactc
     2641 cagaggttaa ccttaacgcc aaactccttg aatgctacga gagtgtcgtt agcaaagcta
     2701 tcgattccca ctacggttaa gcctttcatg cccacgtaaa ggttagggaa acccttaatt
     2761 agcgatacct gaaaatcggt aaagtcaacc tttgcattca gcatgctgtt agcctctttc
     2821 ttggcaactt gcattagctg tggcttaaat attattggca atacaattgc tgcagctata
     2881 aatagcgtaa ccaacgcagc caaaaccttt agagtgatac tgagaatctt tttcatggct
     2941 gtataattat tttagcagta taatcaatgt ttgaactatt tatacaaatt tagtgaatca
     3001 aactgaaagg tggcactttt tttatcattc ttaacagaca catggcttga acaaacacca
     3061 aatacaaatg tttctaacag cgttgtgcca tactaaaaaa agtttaaatt tgcttaatgc
     3121 agaatgccat gaacctcatc aaaagaattt ttcgattcta ctacgaaggt tttaaaagca
     3181 tgaccattgg gaaaacgctt tggttaataa tccttataaa gctctttgtg atgtttgccg
     3241 tactaaaggt tttctttttc ccaaatattc ttaaacgcaa ctttaaaacc gatgaggagc
     3301 ggagcaagta tgtaattgaa cagctaacaa ctaaacctaa atagcaatac gcatggaaca
     3361 tttcgaccta tcgttagtca actggtcgag ggcacagttt gcccttacgg ccatgtacca
     3421 ttgggtattt gtccctctca cgctggggtt ggctttcata atggcgttca tggaaacgct
     3481 gtactacaaa accggcgacg aggagtggaa acggataacc aagttctgga tgacactctt
     3541 tggtatcaac tttgccattg gtgtggcaac cggcattatt ctggaatttg aatttggaac
     3601 caactggagc aactactcct ggtttgtagg cgacattttt ggtgcacccc ttgccattga
     3661 gggtatcatg gcattcttca tggagtcgac ctttattgcc gtgatgttct ttgggtggaa
     3721 caaggttagt aaaaagtttc acttactctc aacatggctt gtggctctag gagccaacct
     3781 atcggcacta tggatacttg tggctaatgc ctggatgcaa taccctgccg ggatgttctt
     3841 taaccccgat acggctcgta atgagatgca taatttctgg gaggtattgc tttcgcctgt
     3901 gtctgtaaac aaattcctcc ataccatatc gtcggggtat gtgctggctg ccctttttgt
     3961 tgttggaatt agtgcatggt accttctgaa aggtcgaaat gtattgctgg ccaaacgaag
     4021 tattattgtt gcctcagtgt ttggattgat aacatctgtt ttccttgccc ttactggcga
     4081 tggctcagcg tatcaggtag cacaaaagca gcccatgaaa ttggcagcca tggaaggtct
     4141 ttacgagggc aaagagggtg ccccactaat tgtaattggc gcacttaccc ccggtaagca
     4201 gtataacgac aataaagacc catacctatt ccggattcaa attccaaaac tcctatcgtt
     4261 cttgggttac cgttcaggcg atgcctttgt tccgggtgtg aaagatattg tggaaggtgg
     4321 atatacttac accgataagg acggcaacca gaaggttgcc ctttcggctg aacagaaaat
     4381 tgaacgtgga cgaattgcta tacaggcgct tgccgactat aaaaatgcaa aggaacttgg
     4441 cgataccaca gccatggctt accaccgtaa gttactggat gcgaactttg agtactttgg
     4501 ctatggttac ctgaacgacc ccaagagcat tataccaaat gttccgctta ccttttatag
     4561 cttccacatc atggttgggt tgggttttct gtttatcgcc ttctttattc taatacttta
     4621 cctagtcttt ttcaaggaat ttaaaattaa caagtggttg cttcgcacgg caattctcat
     4681 gattcccctt gcctacattg ccagccaggc tggctggatt gtggctgagg ttggccgcca
     4741 accctgggta attcaggata ttctgccaac ggttgcagca gtatcacgta tcgatgcgag
     4801 tgccgttcag gttacattct tcctgttctt cataatcttc actacactcc ttatagcgga
     4861 actacgaatt atgttcaccc aaattaaaaa aggacctaaa gatggaggga aatagccatg
     4921 gttgacacaa catacatttt cttacagcac tactggtggt ttatcatatc gctactgggt
     4981 gcgctgctgg ttttcctact ttttgttcaa ggcggacaaa ccctgattta tacccttggg
     5041 aaaacagaca tggagcgaac aatgatagtt aacgctcttg gccggaagtg ggagttcacg
     5101 ttcactacac tggttacatt tggtggtgct ttttttgctt cgttccccct attctactct
     5161 accagttttg gtggagcata ctgggtttgg atggccatac tgttctgttt tgttgttcag
     5221 gcagtttcct acgaataccg cagtaagccc aacaacttct tgggccctaa aacctttgag
     5281 gtattccttt tcataaacgg actttttgga accgtgctac ttggaactgc cgtgggaaca
     5341 ttcttcacgg gttcgcaatt ctctgttgct aaaatcaacc tcacaaacat cagcaatcca
     5401 gttatttcat cgtgggaaac gccttggcat ggacttgagg cagccctaaa cttgcataat
     5461 gttgcccttg gtcttgcagt attcttcctg gcaagggtgt tggcaattct ttacttcatg
     5521 aaccatatca atcaccctga aatttttgcc cgctcccgca aacacttgct gtataatgcc
     5581 attccgttcc ttgttttctt cctaaccttt gtaatctggt tgtttatacg cgaaggtttt
     5641 gcagtaaacc ccaccacagg cgatgttttt atgcagaagc ataagtactt gctcaaccta
     5701 attcaaatgc ctgttgttct ggtgcttttc cttggcggag tggttcttgt gctttggggt
     5761 atagcaaaat cgctggctaa gccatacacc aaggggattt ggtttaccgg tattggtact
     5821 gtgctaactg tactctcgct cttcctgata gctggttata acaacacagc ctactaccca
     5881 tctacattca acctgcagca ttcacttact attttcaata gttcatccag caagttcact
     5941 ttaaccgtga tgagctttgt ttcgttgctt attccatttg ttgcagctta tatctggtac
     6001 gcatggaaga gcatcaatgt taaaaagata gatgaggaag agatgaaaaa cgaaacccat
     6061 gtatactaaa acctactgac tatgagattt ttaaacgaaa tactttggtt tgccagctgg
     6121 ccggtactca tttacttgag ctacttgtta tcggtttggg ctgtaaagtt atgggaaaag
     6181 aagaaccccg atcaggtaaa atgatgtaga tttgcctgca tcaacaatgg tttaatgaac
     6241 atttataagc tacttaaact taaccgcgct attaggagta accgtttaaa gttactccta
     6301 atctttatgg ttcagctgct gaacaggcga tactactcgg ttaggattga ccccatattt
     6361 gcctgcaacc tacgctgcct gatgtgctac ttcagtaaaa gtaggaaagc gcaacccaaa
     6421 acattttcaa aagtagagct tgagctactg gcaaaaaatc tgtttggtaa tgccctacag
     6481 gtaatggttg gatgcggtgc cgagcctacc attcatcctg attttatcta tttagttgag
     6541 ttggccaaaa ggcataatgt tccccacgtt ggaattgtta ccaacgggca actgctttca
     6601 aaaaacgacc tgctaaccct tagcagtgct gggctaaatg agcttactct ctcggttcat
     6661 gctacgcaaa aggaaaccta tgaaaacttt atggtgggcg ccaaatggga aaaacttata
     6721 ggccttcttg aaaatattaa aatccttagg gatgatggca aaattgcttt ttcaatacgt
     6781 atgaacttta cggctaatcc tgataacatt caagagctgg agcaattttt tgatgtgttt
     6841 ggtgactacc caattaatac ccttcaggtt agaccaataa tggaaattgg aggtgcctat
     6901 tttaagccgt ttagctgcga gagcatttca ttgtataaca gtgtaattga atcgctgttc
     6961 actacgtgca aaaaacatgg catcacatta cttgccaata cacaaaatcc tagtagtcaa
     7021 caaaaaagta atccagtaat tgcacaaaca tacaagtatg tatcgccaca ggttgtggtt
     7081 gataacaatt tccgatggca aaccgaaacc tacaggcaat tcttaaaacg aacaggttgg
     7141 cataaagcta tgcttaaatc catattctca atccggctga gtaattcatc taccgacctt
     7201 gcagaacgtt acggtgggca atacgatgtt ttgtaaacaa aacataaatt ttgtggttaa
     7261 ctttgctgct tgaatacaaa aactgaattg gaacccagaa ttgaaatatc gcccttaacc
     7321 gagtggggat taaacctaaa ctacccccta attattgccg ggccatgcag tgccgagagc
     7381 gaggagcagg tacttacagc ggcccatgcg cttgcccgca atgggaacgt tagggttttc
     7441 cgtgctggta tatggaaacc acgaacccgc ccgggaggct ttgagggtgc cggcgaaaag
     7501 ggtttgcagt ggcttaaacg tgtaaaagat gaaactggct tggccgttgc cacagaggtg
     7561 gccatgcctc aacacttgga actggcgctt aagtatggaa ttgatatggt ttgggtggga
     7621 gcacgcacaa ccacaaaccc atttctggtt gagcagctgg cctcggtact tgccggaacc
     7681 agcatcccgg tactggtaaa aaatccaatt actcctgacc ttgagctatg gattggaacc
     7741 cttgaacgcc tttggcgttc aggcatcaca cgcctagctg ccatacacag gggttttcac
     7801 ccatacgaac gttcacgctt acgcaacatc cccaagtggg agcttgccat cgaccttaaa
     7861 agtcgtatcc ctagcctacc cattatttgc gatcccagcc atattgcagg agcggctaac
     7921 cttgtacccg aaattgctca gcatgcactc gacctgtcca tggatgggct aatggtcgag
     7981 gttcatccca acccagcaat tgcccttagc gatgcccgtc agcagcttac ccccgatgcc
     8041 tttacaaata tgctttcgga attaactttc caccagaaca catcggttaa taacgagttt
     8101 gctaacttcc ttgaacaggt tcgcaacaag atagactcca ttgaccagca aattattgag
     8161 ttgctagcaa accgtatgcg gttggttgag cagattggtg agtataaacg gactaataac
     8221 gttacaattt ttcagctacg caggtgggaa aaaattcttg agtcgcgcat tgagtgcggg
     8281 aagcgtttgg ggctcgatga agagtatata aaaagcctgc ttcagctggt tcacaaggaa
     8341 tccataaaga aacaggccga aatattacgt ggcaaccgtt taaccaacgg cgagtaagcc
     8401 ctattcggtt atctcggtta gcttaaaatc gtacccttcc attactgggt tcgagagcac
     8461 ctttgagcat atttcggtta ccttttccat ggccacctca cggtttgctg cttcaatttc
     8521 aaggttgatg tgctttccaa ttcgaacatt ggatacttcc ttgaacccaa tcccatgtag
     8581 ggttgcatta actgccttcc cctgggggtc gagcagagcc ttaagtggca tcacattgat
     8641 ttctgctaaa aacttcattt tatatcgatt tttcttcgga caaaattagc caaaatttgc
     8701 attaatcagc tctgaaaatc tttatttatc aacttatccc ataaatagta gatagttgat
     8761 agaacgatat accaaacaat gataattgaa agcgctgcct tttgaaaaat ggcaataaat
     8821 acaacagctc cagctagaaa ggtaagctgt ttgtaggcca ctgatggttt taacgacttg
     8881 attttaagcg agaacatggg taattcacaa accagtaaaa acgaggatat aattgctaga
     8941 acaatcagga ctacttgcga accaacaatt gggttgaaca aaccactcca gctactgttt
     9001 gcgccgaaaa ccaatccaat tatcagaaag gcatttgctg gtgttggcaa tccaataaac
     9061 gaacttgttt gtcgctcatc aatattgaat aaaccaagac gaagcgaaga aaaaacagcc
     9121 acaagaaaag gaattactaa tacaaagtgc ccctcaaaaa tgccatgaca atcactaatt
     9181 ccaatgctct ggcgaagcat gtgataaaga attacggctg gcgcaacccc aaagctaacc
     9241 acatcggaaa gcgagtcaag ttccttgcct agctgggtat aagcaccaat taaccttgcc
     9301 gtaaaaccat ccaggaaatc aagaacaccg gaaataatga taagcaaacc agcaagttcc
     9361 agtcgacctt cgaatgctgc cacaattgaa aggctacccg aaaataggtt tagcagagta
     9421 attccattgg gaatatgtct tataacgcgg gttattctgt tcatatacaa aattaattca
     9481 ttcaaatttt agcttttggt atatcgctcc atcaaccagg ttgctggttt ccataatgca
     9541 aaacccacca cgctgccaag taatgcacct acaattacat cggcagggta atgcacccca
     9601 aggtatatac ggctatacga aacaaacgct gcccaaatga gcaaaccaac tcctgcccaa
     9661 cggttcctgt atagcatact caaaaagact gcaacaccaa atgtattggc tgcgtgcgac
     9721 gaaacaaaac caaacttacc cccacatcta cccacaagat ggattacacc acttaaatcg
     9781 gggttgtggc atggccttaa cctttgaaaa acatttttaa agagatgtac tgagagctgg
     9841 tcggctacaa gaacaacaac tgcaattgcc agcagccacc aaagggtttt ccacttatgc
     9901 tttcgggcaa tgaagtagat tagcaacagg taaaacggta accatgtgag ctttcccgat
     9961 gcaaaaagca ttacactatc ccagaaaggg ctgtgcaacc cgttaaggaa aagaaatagg
    10021 tcggtgtcga gttttaccag gtaatcaatc ataattactg ttggtttagg ttatcccaaa
    10081 gcacatcctt taaccgctca ataccctcgc cggttaccga cgagataaac acagcaggta
    10141 tatcgggcag atgctttgat attttctttt tagcctgttc gtccaccagg tcgcatttag
    10201 tgatggcaag agcccttttc ttatcgagca gttccgggtt gtacattttt agttcgttca
    10261 ccagtatttg gtactgcccg taaatatcgt cggaatcggc tggcaccatg aagagtagca
    10321 tggagttgcg ctcaatgtgg cgtaaaaagc gaagtcctaa ccctttcccc tcatgggcac
    10381 cttcaataat accagggata tcggccatta caaacgacct atcgtcgcgg tatcgaacaa
    10441 ttccaaggtt tggtaccaag gtagtaaaag ggtagtcggc aattttgggt ttggctgcac
    10501 tcaccaccga cagcaatgtc gatttaccgg catttgggaa gccaaccagt cccacatcgg
    10561 ccagtatttt taattccagg ataacgtagc cctcctgtgc gggttcgccg ggttgagcaa
    10621 acctgggggt ttgcagggtt gccgtccgaa agttccagtt acccaaacct ccgcgtccac
    10681 cctttaaaat catctctacc tggccgtgct cggtaatttc agcaatcacc tcattggttt
    10741 cggcatcgcg ggcaatggtg ccaatgggca cctcaatgta aacatcctcg ccatccttac
    10801 ccgtacaacg ggcaccactc cccgattcac catcgcctgc aaaaatatga cgttgatatt
    10861 tcagatgaag taaagtccac agctggctgt tccccttcag gtaaatatgg cctccgcggc
    10921 caccatcgcc accatcgggg ccgcctttgg gtacaaactt ctccctgcgg aagtgtacgc
    10981 tccccgcacc acccttgccc gaacggcaaa atatcttaac gtaatctaca aagttcgagt
    11041 ttgacatgtc ctgcaataaa ctacccgcaa agttacttta aaaaggtgga atgggaaagc
    11101 agaacaaaaa aatgatgctg gctagcagta aaacaacaat cgcatggaat tctattccgc
    11161 acgaacgggt agtcatagca taaatgtaac cgtattggaa agagcacacg catcccccca
    11221 tgcgcacgaa cattagaata ccgaacactt atttaacaaa aatcgggttg tttaattatc
    11281 ccaatttagt tgaaattgac cataaggtta agggaacgat aggcctatac acgataaaat
    11341 ggttaatagc aagtacattc taatctgctc atatctgtcg gatgttttgt ttcgggattt
    11401 taaaatggtt attataatgc aaaaggcaaa gtaaagcaaa ctcaaaatcc tgcctgtatt
    11461 agggataaaa aatgttatta caaacgcaat ggggaataat gaaagcgccc agcgggttgc
    11521 tatgtttaat agcatactat cgccgcttat gttattcctg tagaagaata aatagtaacc
    11581 agacagtaga attgaaagaa agataattac tagtagtaat ttatcactgc tggtaatacc
    11641 tttaagaatt cctgctaaaa ctaaaccaaa cactagcaga atttcagtaa gctttggctt
    11701 catatttcgt ataaattaga ttggttaaat gcattaacat tataatcaat tttacaaaat
    11761 tcatacccac gttggtgcga gcctcaatat gcgtacgata tatacaaaac caacccaaaa
    11821 atttcagcaa atctagtaaa aaaaatttct cgctcgtgct ttcgagcagt ttttcatcta
    11881 taccggccaa ggcgcaatgt taggttacgc tcaaacttgc ctttggaggg gcaaccaaca
    11941 aaaatggacg gacgactttg aaaaaactca cacggaatac aataacacag gggggcacat
    12001 cataaatgat acagcattaa aaagagcgca cgcgtcgggg gacgcgtgcg aacaagtatc
    12061 aatttttatt taactctttt atgatttgta acgctctatc gcgcaatggc ttttgtcttt
    12121 ttcgccagag taaatatcca ataaatccag acattatgaa aattggtaaa agaatggctt
    12181 gtacaattaa tatgcgattt aatggttcta ccgatgtcca tcgataatat tctgaaatcg
    12241 taatcgcaat atctataaaa ataagcgaag gtaaaacaag taagtatctg gaaaaagtta
    12301 aattgtgtcg ttctgctgct ttcttgaaca tttcaaggga tgatatggaa taatcaatct
    12361 tgctgaactc attatgatac tttctgaata ataacgcaaa atatgtaaat gatagcacat
    12421 agaacaaacc tgatatccta tgatgaagtt gcaaatcagg atcgggattt actatcataa
    12481 ataatgtgta aataattatc attgtgaaaa ataatctttt tagacccttc attttttgga
    12541 ggtttcgagc atcttcattt ttcaatttaa tcaataaagt atcgatgtca atagaattat
    12601 tttgttcatg gctatcggga attctattgc tattgctgtt taggttgatc tgattattca
    12661 tagttttctc ctttcatttg gtttcgtaaa ttttctttta tcctgtgaat tttgatacgg
    12721 acattaggct cggtgatacc aataatatcg gctatttcct tgcttgttaa ttcatcgagt
    12781 acaagcccca ttatcgcctt atcaattaca ctgagttggt tcagatgggt ttgcaatctc
    12841 tgaaattgat catcttttag catattatca ttaaattcag tagataaaaa gtttttaatg
    12901 tttgtgtttt caatatcaac atttagtttc ataagcttat actgtttgcc tgcataactc
    12961 aaggaagtgt taacagcaat tctataaacc caagttgaaa cctttgagtc gccacggaat
    13021 gagtccagac tcttccatat atttattaat atttcctggt acatatcttt ttgatcttca
    13081 gcagacggtg cataatacct gcatattcgc attatttttt ctttgttttc gtcaactgtt
    13141 cttttaaatc tttcttctct actcatatag ttgaattatt tttttacttt ttcatttaga
    13201 cattcatcag agtatttcgt tacaaaattt tttgtaaaca tttacattag cttcctcttg
    13261 ttctcgtaaa tagcatacac gtttgcccag atggctatgc caaactcaat tcggggcatt
    13321 aaaatacgca caagcgtata atgattaatg atattcattg aaataaaata ggccatcgtg
    13381 gttgtcgatg gccttgatgt attaattagg gtttgttacc tgctactgtc aatcacctgg
    13441 caaattcgct caaatatttc atcgatagta cccattccgt taacgccacg gtacttgccc
    13501 tgcgccttgt agtaatccat taccggcagg gttgttttgt tgtagatgtc gagccggcca
    13561 cggataatac cttcgtcctg gtcatcgctg cggtcactca tttttccccg gtttagcagg
    13621 cgagctaaga gttcatcgta ctcaacctcc aaagctaaca ttatggtaat gggagccttt
    13681 ttggcatcga gcatttcgtc gagtttcttg gcctgtacgg tagttcgggg aaatccatcg
    13741 aaaacaaagc ccggaacatc gtggtggtta tcgagttccc cggcaatcaa attgattacc
    13801 atctcatcgg gaaccagctg ccctttatcg atatactttt gagcctctac tccaattggc
    13861 gattgttcac gtattgcagc cctaagcata tcgccagtag ccagatgctg cagcttgtat
    13921 ttctcaatta ttttttggga ttgagtgccc ttgcctgcgc ccggggggcc aaataaaaca
    13981 atgttaagca ttttacgata tattgtgttt tggtttatta ctttttagtt tccaacctct
    14041 tacctgctaa aatgcttgaa caaatatagg taaacaattg tcacggccaa aaaattattg
    14101 gacaatttgc gggttgactt aaagcattac cacagaaaaa tattccacgc tttttaaagt
    14161 tcattaagtt ttatcaaatt tgatttgcag catatagatt attttaccat gcaattttta
    14221 atatatagaa ttttagtaac agttcagaga tagtaacagg ttaattgaat gtaactaaaa
    14281 cggcctaaca agatgttgtg cataagcaat gtatccataa ctaatattgc tctctaaagc
    14341 ttttttacaa acttgagatg tgtttgctcg tatcccaacc gtttgtaaaa ttcctgtgtt
    14401 cgcaccctgt aatttcgggt ggtaagttca acctcttcaa gaccgagctc tttggcaagt
    14461 ttctccgctt gctccatcag catgctgcca accccctgcg agcgatactc gggcaaaacg
    14521 tttagctcct ggagctcaca ggttaacctc acgtgatgca gaatacgctg aacatgcatg
    14581 ctcaagaaac ctataacctt ttcgccatgg caggctaccc agtaatgcac cgtgctatct
    14641 tcaagattcc ttcggtaatt ctcagcaaag tatgagtaat tgaggctctc accctcaagg
    14701 tcgcaaatta gctggtaaac cggttctata tcggcaagtg ttgcctttcg aattgtaaac
    14761 atgggcttta ctttaaggtt tcaactacca tgcgtagtag cgcgatcatg cgttgagcat
    14821 tttgttcaaa aatgcgaact atctcctccc agctcacggg tggttcatcg gttttccagc
    14881 aatcgtaatc ggtgctcatg gcaatggcag catagggaat ggccagctcg ttggccaggg
    14941 ccacctcggt ggcaaccgac atgttaatta catcggctcc ccagctgcga aacatcattg
    15001 attcggcccg ggtggaaaag cgtggcccct caatggtaac cacggttccc cgtggatgac
    15061 acttaacccc taccctgccc gcacaatcaa ggatatgggc gcgcaatgcc gagtcgaagg
    15121 gatcggccat gggagtatga accgcaccac tggcaaagga ttcgtggtag gtattaatac
    15181 gctttcgggt aaaatcaatg aactggtcaa gtaaaacaaa gtcgccccgt tcaatctctg
    15241 ctcgtaaact tccgcaagca gtagtggcta taatgcaatc ggcaccctga tacttgagcg
    15301 cgtaaatatt tgcccggtaa tttacctcag ttgggctata ctcatggcta tggccatggc
    15361 gagcaagaat aatcaccctg ttactgccaa tagtcccaac cctcagcagc gaagatggat
    15421 tcccgtaggg agtatctacc ctaagcgtgg tggcatcttt aataatatca gggttatcga
    15481 gcccggagcc gccaataatt ccaattgtag gcatatacat tagtattaaa catcattgcc
    15541 gtgtagttag attatttgtc atgctgagct cgccgaagca tctctttact ctttttctct
    15601 tcagtccttt gatgagatcc ctcgactttg ctcgggatga caaaaaccct tacttccctt
    15661 ctgctccatt gtcatgctga gtcccgattt atcgggatag acttcaacga agcatctcct
    15721 tgcacttttt tctcttttag cattgactga gatccttcgt ttcactcagg atgacaaaag
    15781 gcatgtttat ttttattaac tgaacgactt tttaatttaa catagtaaat ttaatgcaaa
    15841 tcagtcaaaa aaagcatttc cgactacaat tacattgatt ttttcaaaca gctttttacc
    15901 tttgaca
//
LOCUS       DAOSAL010000060        15847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_2542_length_15847_cov_50.426798, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000060 DAOSAL010000000
VERSION     DAOSAL010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 15847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15847
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2542_length_15847_cov_50.426798"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..3113
                     /locus_tag="PK049_08595"
     CDS             75..3113
                     /locus_tag="PK049_08595"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOK61657.1"
                     /translation="MRITGTVISADGAWPLQGVTVVVVGSTIGTVTSMEGRYEITVPA
                     IAKKLRFSYIGYKTVEVEIANRTVIDVEMEEEAIEVKEVTVTALGIMRAEKATGYAIQ
                     SVKGEELARTPELNIVNSLQGRISGAIITNTSGAVGASTRIVLRGVNSLSTDNQPLFV
                     VDGVVLNNTNFGGTYTEGVNRGSGIADINPNDIESVTVLKGPNAAALYGSRAANGVII
                     IKTRSADTDKRFGVSFNSTTTFERPLRLPDFQNVYGQGTNGQFEFYDGKGGGINDHVD
                     ESWGPKLDIGLMIPQWNSPVVGGVRQPTPWVSHPDNVKNFFELGKTFSNNIAIESAAD
                     NYAVRFSYTNSMQTGMIPNTDQEKNSVNLNATASPYKFLAFTANATYINTYSKNLPAY
                     GYSGQNVMQQFMWFGRQVDVADLKQYTYPDGSKRNWNYSYHNNPYFTLYENLNGIDRD
                     RLLGQATSTIKFTDWLSLKTGIGVDYYTNYNNARSAYGDIESPYGFLSESKRTFREVN
                     TDFMFMINKSFTEKLAFSFNLGGNRMEQFTQSLSAYITELSVPDVYTFENAKVPVVTM
                     NYITKKRINSLYFNGQFIWKNALFFDFTGRNDWSSTLPAGNNSYFYPSVNVSAVLTDL
                     FDIQSRKLSFAKLRAGWAQVGADTDPYQLQPVYRFDDGWNSGTKLAQIYIPNDLPNPN
                     LKPQRTNSFEIGADLRFFMNRLAFDLTYYSQSTYDQIIRMPISTTTGYTSTIVNAGQI
                     DNKGVELSVNGIPIKTRSGFEWNIGVNFSRNVNKVRELAEGLDQYVLGGYWSLDVLAI
                     PGEPFGVLYGYDFMRNDEGKVIHIDGLPLQGDIKKLGNYTPDWVAGVTNSVRYKNLTF
                     SALVDARWGGEIYSMTNTWGRYAGVLKETLKGREGGIVGVGVMPDGQGRWTENDVVVS
                     AEEYNKAAYNNNIAYSSIFDASFVKLREVQIGYTIPKLGKSSIRNISISLVGRNLAIL
                     YARVPHIDPETAFSNSNVQGLEFGQHPTARSIGVNLSFKF"
     gene            3133..>3359
                     /locus_tag="PK049_08600"
     CDS             3133..>3359
                     /locus_tag="PK049_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61658.1"
                     /translation="MKLYRLFTLSIAVLGVVVLQSCTKDFDEINKNPNAIDINKDYPD
                     KLLANAIEDIADRVFDVWLGHEIGSCWVQHMA"
     assembly_gap    3360..3459
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3460..4089
                     /locus_tag="PK049_08605"
     CDS             <3460..4089
                     /locus_tag="PK049_08605"
                     /inference="COORDINATES: protein motif:HMM:NF024152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HOK61659.1"
                     /translation="AGGYWIGLPNGLTSAKAAAYLGNGIKRTSKLGEYFRKADAPGMF
                     MSRAEVCFLLAEAKHRGLLTFGTETQDYYELGVKESYRQFANEIIENVPYYYDLGGAS
                     RTIDDYFNDFLANSGKWEVGKELEKIYTQKWVAMFDQGLQAWFEWRRTGYPQLTPAED
                     GLNGGRIPQRLRYPSDEYSRNGTNLAAAVARQGADNLNTRVWWDVADNN"
     gene            4115..>4593
                     /locus_tag="PK049_08610"
     CDS             4115..>4593
                     /locus_tag="PK049_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61660.1"
                     /translation="MRLFDTKVIKGALLLAVFAMLFSSCLMNDDLMTADAKTGGLVES
                     VPLVQLVPKKSNTVDIELKVYLGPAIQEVKVYKQFYHNATDEASNVELLGSFSVSGQN
                     LNDTLLLSQTFTWEQLKQGLTLDSDTLPDDPLNADIGDYFMLKYVSVMDDGREVTSNS
                     "
     assembly_gap    4594..4693
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4694..5928)
                     /gene="pepT"
                     /locus_tag="PK049_08615"
     CDS             complement(<4694..5928)
                     /gene="pepT"
                     /locus_tag="PK049_08615"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HOK61661.1"
                     /translation="MINKQSLIDRFIRYVKIDTQSDDTVTERFPSTEKQLVLSNLLVK
                     ELKELGAQEVEIDQYGYVMATIPANTSKKVPVLGFLAHVDTSPDMPGKDVNPRFVENY
                     DGSEIILNKELGIALSPKEFPELKNYVGQTLIVTDGTTLLGADDKAGLAEIMTAVEYL
                     IKNPSFEHGKIRIGFTVDEEVGRGVDHFDVKRFGADFAYTLDGGAIGELEFENFNAAS
                     AKISIQGRNIHPGYAKDKMLNAILIAQEFNSLLPAHERPEHTQGYEGFYHLIKMDGSV
                     EKAAFQYIIRDHDRKKFEARKAYMQRIAEYLNTKYGEGTVTLELKDQYYNMREKVEPV
                     YHVISTAIEAMEAVGVKPKVKPIRGGTDGARLSYMGLPCPNLFAGGENFHGKHEYVAV
                     ESMVKATEVMLKIIELYAS"
     gene            complement(5960..6346)
                     /locus_tag="PK049_08620"
     CDS             complement(5960..6346)
                     /locus_tag="PK049_08620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOK61662.1"
                     /translation="MIDYKQLDVWIASRQLANSIYTISKYFPKDEIFGLTQQIRKSAI
                     LIPSNIAEGIGRNHLKETTQFLYIARGSLFELETQIYIAFDQGYIDATALEQITNDIE
                     KCKKLLNGFINYYLKKKHEDHTNTKN"
     gene            complement(6411..8396)
                     /locus_tag="PK049_08625"
     CDS             complement(6411..8396)
                     /locus_tag="PK049_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HOK61663.1"
                     /translation="MSHEPVEKIKGLPENAYTELREGEEYQPVMPPSKAVPEVTAWSV
                     IWGLIMAIVFSAAAAYLGLKIGQVFEAAIPIAIIAVGLSTLAKRKGALGENVIIQSIG
                     ASSGVIVAGAIFTLPALYILNLEAEFYQVFLSSLFGGILGILFLIPFRKYFVADMHGK
                     FPFPEATATTEVLVSGEKGGKQARLLVVSGLIGGIYDFIIATFGWWSEVVTTRVIPAG
                     EALAEKAKLVFKLNVGAAVMGIGYIIGLKYTAIIAAGSFVGWFVIIPIINYFAPGLTE
                     AVGSNITLTVGQMSAEQIFSNYVRPIGIGGIAMAGIIGIIRSSGIIRKAFGLAVSELT
                     GKHSAIPKEELRTRRDLPMSIIAGGILFTTILLLVFFYFGVVHNITHALIALGIVMVI
                     AFLFTTVAANAIAIVGTNPVSGMTLMTLIITSLVMASAGLSGTSGMIAALIIGGVVCT
                     ALSMAGGFITDLKIGYWLGSSPYKQQTWKFLGTFVSAATVGGVIILLNKTYGFTGKDA
                     LVAPQANAMAAVIEPMMSGKPAPWALYAAGAFLSLILTMIGVPALAFSLGMFIPLQLN
                     TPLLVGGIIAHIVSTRSKDNSLNRARKERGTLIASGFIAGGALMGVVSALLRFGGINY
                     VNPEWFESHSAEILGFVMFLILCGYLIWESLRAKVEE"
     gene            8815..9021
                     /locus_tag="PK049_08630"
     CDS             8815..9021
                     /locus_tag="PK049_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61664.1"
                     /translation="MGTPRVELPRDTIRHACGVTHLVSLTKPTATFHSRPIAGSGFYA
                     PFYAILCWFINLIKRVEGTKRCLG"
     gene            9675..10856
                     /locus_tag="PK049_08635"
     CDS             9675..10856
                     /locus_tag="PK049_08635"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HOK61665.1"
                     /translation="MISLDKAIRIVEQSVTALNGQEHVSLSESLNRVLRHDVVSDINM
                     PPFRKTAVDGYACRAADLDNPLTVIEVIAAGTIPQRTIAQGTCSKIMTGAPIPNGADC
                     VIPVEDTEILSDGTIRFKGSKPKSNICELGEDIKIGESPLSKGTLIKPQHIAIMAALG
                     CHSPIVSVRPRVSILPTGDELVEPTEIPQGSKIRNSNGHQLIAQVISAGAIPMYHGIV
                     GDTEEVTEKAIARALAESDVVVLTGGVSMGDFDYVPRIMERLGVKILFDSIAVQPGKP
                     TTFGVAGNKLIFGLPGNPVSSFVQFELLVKPALLMMMQCNEPYARTYKLPLAIDYSRK
                     RAERLGLFPVFVNDIGEVEPLEFHGSAHIFSLAKATGIAAIPLGVKELKKGELLDVRP
                     I"
     gene            10840..11640
                     /locus_tag="PK049_08640"
     CDS             10840..11640
                     /locus_tag="PK049_08640"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOK61666.1"
                     /translation="MFDQFNRRINYLRISVTDRCNLRCTYCMPEEGIVQIPHNQVLTF
                     EEIVEIARIAVQHGFDKVRITGGEPLVRKGIVDLVRMLKTVEGITDLGLTTNGTLLKQ
                     FAQPLYDAGLKRINVSLDTLDPQKYKAITRVGTLSDVLEGLEEAKKVGFNPIKLNCVV
                     ENNSNEPDAQAVKAFANSNGFIARFIPKMNLDDGVFGVVEGGEGGNCSTCNRLRLTAD
                     GILKPCLFSDKGYNVRELGIQQAIEMAIKNKPACGVANRSGEFYNIGG"
     gene            11642..12094
                     /gene="moaC"
                     /locus_tag="PK049_08645"
     CDS             11642..12094
                     /gene="moaC"
                     /locus_tag="PK049_08645"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HOK61667.1"
                     /translation="MELSHTDKNGKANMVDVGHKPNQIREASATGFIRLLPQTVKLIR
                     ENQMKKGDVLTIAEIAGIQAAKRTPELIPLCHTLMLTKVDVKATLCENGVEVKSYAKC
                     IGQTGVEMEALTAATVALLTIYDMCKAVDKQMVIERVELIEKAKKDIV"
     gene            12097..13044
                     /locus_tag="PK049_08650"
     CDS             12097..13044
                     /locus_tag="PK049_08650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HOK61668.1"
                     /translation="MEQTNPITVKVVSVNISEKKGTTKHAVDRIELNSLGVKGDAHAG
                     AWHRQVSMLGVESIDKFTKASGLTVKYGDFAENITTQGLELYRTKPGDRFVGAGVELE
                     VTQIGKSCHGDGCAIYRQVGNCVMPKEGIFVRVLKGGPLKAGDELSFYPKVYRVKVIT
                     LSDRASRGEYEDLSGPEVAKLVSDFFNSVSWQLEVETEIIPDDRDLLENRLIEARNSK
                     YDMVLTTGGTGIGPRDITPDVVKPLLDKEIPGIMEHIRLKYGAEKPNALLSRGVAGLM
                     GTTFVYTLPGSVKAVREYMGEISKVQKHLFLMLMGIDSH"
     gene            complement(13279..13635)
                     /gene="trxA"
                     /locus_tag="PK049_08655"
     CDS             complement(13279..13635)
                     /gene="trxA"
                     /locus_tag="PK049_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOK61669.1"
                     /translation="MIEHLTAETFKQKVFDYEKNNEWKFEGDKPAIIDFYADWCGPCK
                     MVAPILEELAKEYEGKLNIYKVDTEEEQELAAVFGIRSIPSLLFIPLNDQPQMAMGAL
                     PKDTFKKAFKDVLGVE"
     gene            13853..15292
                     /locus_tag="PK049_08660"
     CDS             13853..15292
                     /locus_tag="PK049_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOK61670.1"
                     /translation="MKEYLKSNIFRVISDVAEIEGVDAYVIGGFVRDILLNRPSKDID
                     IVVVGSGVDFARKVAQRLRARKVTYFKNFGTAQLKHNEWDIEFVGARKESYRLDSRKP
                     IVENGSLEDDQKRRDFTMNALAIGLNKRNFGELIDPFNGLQDLKSKTIRTPLDPATTF
                     SDDPLRMLRAIRFATQLNFDIELKTLDAIREMSSRITIVSIERVVDEINKMMASPRPS
                     VGWLLLEQTGLLQHIFPELAAMKGVEARNGVSHKDNFYHTLEVLDNLSLKSDNLWLRW
                     AALLHDIGKPKTKRFVAGQGWTFHGHEFLGAKMIPEIFRKLKLPLNDKMKYVQKMVLL
                     HLRPIVLSQEVVTDSAVRRLLFDAGDDIDDLMMLCEADITSKNDITVRKHLANFRLVR
                     KKLKEVEEKDSIRNFQPPVTGEMIMETFNLKPCKEVGLIKNRIKDAILDGEIRNDFFE
                     AYTLMLNAGKELGLTAININPQYISLTNK"
     gene            15453..>15847
                     /locus_tag="PK049_08665"
     CDS             15453..>15847
                     /locus_tag="PK049_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61671.1"
                     /translation="MHEDLYAPFDFPVYKTEQELFAEKSKLLKEFKSFYRYDSTIHPQ
                     QLEGFRSSFDLAFDKFINSQNVSKTSRAKYLKVKPELEQFVADNISQIYLKGIINQDE
                     SAISNSMLKQGLAVVINNVAYDKWFDEVYT"
BASE COUNT         4522 a         3219 c         3545 g         4361 t
ORIGIN      
        1 ttatgaggaa actgtgtgcc tttctcacag cttttttgtt tataggttct ttgaatgtat
       61 tagcccaaca agctgtgaga attacgggaa cggtaatttc ggctgatggg gcttggccct
      121 tgcagggggt tactgtggtt gttgttggta gtacaatagg tactgttacc agcatggagg
      181 gacgatatga aattactgtt ccggccattg ctaaaaagct tcgctttagc tacattggtt
      241 acaaaacagt ggaggttgaa atagccaacc gtactgtaat agatgtggaa atggaggagg
      301 aagccattga agtgaaagag gttacggtta cagcgttggg tatcatgcgg gctgaaaaag
      361 caacggggta tgccattcag agtgtaaaag gcgaagagtt agctcgcact cctgagctga
      421 atatagtaaa ttcattgcag ggacgaattt ccggcgcaat cattaccaac acatctggtg
      481 cagttggggc ttcaacgcgt atcgtgcttc gtggggttaa ctcgctaagt accgataacc
      541 aacccctttt tgtggttgat ggggttgtgc ttaacaacac caactttggt ggtacttaca
      601 ccgaaggggt aaaccgcgga agcggtattg ccgacatcaa tccaaacgat attgagagcg
      661 ttaccgtcct taaagggccc aatgctgcag cgctttatgg atcgcgagct gccaatgggg
      721 ttatcattat taaaacccgt tcagccgata ccgataaacg ctttggcgtt agctttaact
      781 caaccacaac attcgaaagg cctttgcgtt tacccgattt tcagaatgtt tatgggcaag
      841 gaacaaacgg ccaatttgaa ttttacgatg gtaaaggtgg tggcatcaac gaccatgtgg
      901 atgagagttg ggggccaaaa ctcgatattg ggctgatgat cccgcaatgg aactcgcctg
      961 ttgttggcgg tgttcgtcaa cccacaccct gggtgtcgca ccctgataat gtgaaaaact
     1021 tttttgagct tgggaaaact ttttccaaca acattgccat tgagagcgct gccgacaact
     1081 atgcagttcg cttttcctac actaactcca tgcaaacggg catgattcct aatactgacc
     1141 aggaaaagaa tagtgttaac ttgaatgcta cagcatcgcc ttataaattt ctggcattta
     1201 ctgcaaatgc aacgtacatt aatacctata gtaaaaacct tcctgcatac ggttatagcg
     1261 ggcagaatgt tatgcagcag ttcatgtggt ttggccgaca ggttgatgtg gccgatttga
     1321 aacagtacac gtatcccgat ggtagcaagc gtaactggaa ctacagctac cacaataatc
     1381 cttactttac cctttatgag aaccttaatg gtattgatag ggacaggctg ttgggtcaag
     1441 caacatcaac cataaagttt actgactggt taagcctaaa gacaggtata ggagttgatt
     1501 actacacaaa ctataacaat gcgcgttccg cttatggcga tatcgaaagt ccctatggtt
     1561 ttctgagcga gtcaaagcgc acgttccgtg aggttaatac cgactttatg ttcatgatca
     1621 ataagtcgtt tactgaaaaa cttgctttct cgttcaattt ggggggcaac cggatggaac
     1681 agttcaccca atcgctgagc gcctacatta ctgaacttag cgtacccgat gtttacactt
     1741 ttgagaatgc caaagttcct gttgttacca tgaactacat taccaaaaag cgaattaaca
     1801 gcttatactt taacggtcag tttatttgga aaaatgcatt attctttgat tttaccgggc
     1861 gcaacgactg gtcgagcacg ttaccagcgg gtaacaattc atacttttac ccctcggtta
     1921 acgtatcggc cgttcttacc gacttgttcg atattcaatc aagaaaatta agctttgcca
     1981 agctacgtgc cggttgggca caggttggcg ccgataccga tccctaccag ctacaacccg
     2041 tgtataggtt cgacgatggt tggaattccg gtaccaagct ggctcaaatt tacatcccaa
     2101 acgacttgcc aaatccaaat cttaaacccc aaagaaccaa ttcgtttgaa attggtgccg
     2161 acctacggtt tttcatgaat cggcttgcat ttgacctaac gtattactct caaagcacct
     2221 acgatcaaat tatccgtatg cccataagca caaccaccgg ttatacctcc actatagtta
     2281 atgctggtca aattgataac aaaggggtag agctgtcggt aaacggaatc cccattaaaa
     2341 cccgttcagg ttttgagtgg aatattggtg tgaacttttc aagaaacgta aataaggtca
     2401 gggaattagc cgagggcctt gatcagtacg tgctgggtgg atactggagc cttgatgtgc
     2461 tagcaatacc cggcgagcct tttggtgtac tttacgggta cgattttatg aggaatgatg
     2521 agggtaaggt aattcatatc gacggattac ccttgcaggg cgatatcaaa aagcttggca
     2581 actacacgcc cgattgggtt gcaggtgtaa ccaactcagt tcgttataaa aatctaacct
     2641 ttagtgctct tgttgatgca cgttggggtg gagagatcta ctccatgacc aatacctggg
     2701 gccgatacgc aggggtattg aaggaaactc ttaagggtcg tgagggagga atagtaggtg
     2761 ttggcgtaat gccagatgga cagggtagat ggactgagaa tgacgtggtt gtctcggctg
     2821 aagagtacaa taaagccgcc tataataaca atattgccta ctccagcatt ttcgatgcaa
     2881 gctttgtaaa gcttagggag gttcaaatag gctacaccat acccaaatta ggtaaaagct
     2941 ccatacggaa tatttccatt tcgctagtgg gtcgtaactt agccatactt tatgcaaggg
     3001 tgccccatat tgaccccgaa actgcattta gcaactccaa tgttcagggg ttagagtttg
     3061 gtcaacatcc tacagcccgc agcataggag tgaatttgag ttttaagttt tagcttgata
     3121 aaaaaaagag gtatgaaatt atatagatta tttacgttaa gcatagctgt actgggggta
     3181 gttgtcttgc agtcgtgcac aaaggatttt gatgagatca acaaaaaccc caacgcaatt
     3241 gatatcaaca aggattaccc cgataagcta ctggcaaatg ccattgagga tatcgccgat
     3301 agggtatttg atgtatggct ggggcacgag ataggctcat gctgggtgca acacatggcn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng cgggaggtta ctggataggg
     3481 ttacccaatg gtttgacatc ggcaaaggct gccgcttacc tgggcaatgg tattaaacga
     3541 acctctaagc tgggagaata cttccgtaaa gctgatgctc cgggtatgtt catgagccgt
     3601 gcagaggttt gcttcttgtt ggctgaagct aaacatcgtg gtttgttaac atttggaact
     3661 gaaactcagg attactatga actgggtgtt aaggagtcgt acaggcagtt tgccaatgaa
     3721 attattgaaa acgtgcctta ctactacgat ttgggtggtg cttcacgaac cattgacgat
     3781 tattttaacg attttcttgc aaatagtggt aagtgggaag ttggtaagga acttgagaaa
     3841 atttacaccc agaaatgggt tgccatgttc gaccagggct tgcaggcatg gtttgaatgg
     3901 cgaagaacgg gttatcccca gctaacaccg gccgaagatg gattaaatgg aggaagaata
     3961 ccccaacgct tacgttaccc ttccgatgag tattcccgta acggtaccaa cctggctgca
     4021 gctgttgccc gccagggtgc cgataattta aataccaggg tatggtggga tgttgccgat
     4081 aataattagt tgaactctat taattgatga gaatatgaga ttgtttgata cgaaagtcat
     4141 aaaaggggcc ttgcttttgg cagtatttgc catgcttttt agctcctgcc tgatgaacga
     4201 cgatttaatg actgccgatg ccaaaaccgg tggcttggtt gaatcggttc cgctggttca
     4261 gctagtgccc aagaaatcaa atactgttga tatcgagctt aaggtttatt tgggaccagc
     4321 catacaagag gttaaggttt acaagcagtt ttaccataat gcaaccgacg aagcctctaa
     4381 tgttgaacta ctcggcagtt ttagtgtaag tggccagaac ttgaacgaca cattgttgct
     4441 tagccagacc tttacctggg aacagctgaa acagggatta acccttgata gcgatactct
     4501 acccgatgac cctttaaacg ccgatattgg agactatttc atgcttaagt atgtttccgt
     4561 aatggacgat ggcagggagg ttaccagcaa ttcnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnntttgatg cgtatagctc aattattttg agcattacct cggttgcttt
     4741 aaccatactt tctacggcta cgtattcatg ctttccatga aagttttcgc cgcctgcaaa
     4801 taggttgggg caggggagtc ccatatagga aaggcgggcg ccatcagttc ctcctctaat
     4861 gggtttaacc tttggcttaa cgccaactgc ctccatggct tcaatggcag ttgaaataac
     4921 atggtaaact ggctcaacct tttcgcgcat gttgtagtac tggtccttta gctccaaagt
     4981 aacagtacct tcgccgtact tggtatttaa atattctgct atgcgctgca tataagcctt
     5041 tcgggcttca aactttttac gatcgtggtc gcgaataatg tattgaaaag ctgccttttc
     5101 aactgatcca tccatcttga ttaagtggta gaacccctcg taaccctgag tgtgttcagg
     5161 gcgttcatgt gcaggtagca ggctgttgaa ttcctgggct ataagtattg catttaacat
     5221 cttgtccttt gcatatccag gatggatatt acgtccctgg atggatatct tggcgcttgc
     5281 agcattaaag ttctcaaatt ccagttcacc aatagcgcca ccgtcgagcg tataagcaaa
     5341 atcggcacca aagcgcttta cgtcaaagtg gtcaactccg cggccaacct cctcatcaac
     5401 tgtaaaacca atacgaattt taccatgttc aaagctgggg tttttgatga ggtactcaac
     5461 tgctgtcata atttcggcta atccggcctt atcatcagcg ccaagtagcg tagtgccatc
     5521 ggttacaatc agggtttgcc caacataatt cttaagctca gggaattcct taggcgaaag
     5581 tgcaattcct aactccttgt tcagaataat ttcggaccca tcgtaatttt caacgaagcg
     5641 tggattaaca tctttccctg gcatatcggg cgaagtatca acatgggcaa ggaaacccaa
     5701 aaccggaacc ttttttgagg tgtttgcggg tattgttgcc atcacataac catactggtc
     5761 aatttcaact tcctgagccc ccaactcctt taattccttt actaaaagat tgcttaaaac
     5821 cagctgtttc tcagtgctgg gaaacctttc ggttacagtg tcgtcgcttt gggtatctat
     5881 ctttacatac ctgataaagc ggtcgataag agattgtttg tttatcatgg cggattattt
     5941 agtttgtagt ttgtagtgtt tagtttttag tattagtgtg gtcttcatgt tttttcttaa
     6001 gataatagtt gataaatcca ttaagcagtt tcttgcattt ctcaatgtca ttcgttattt
     6061 gttcaagagc tgtagcatca atataacctt gatcaaaagc tatatatatt tgcgtttcca
     6121 attcaaataa cgatcccctt gcaatgtaaa ggaattgggt tgtctccttt aaatgattgc
     6181 ggccaatgcc ttccgcgata tttgaaggaa ttaaaatggc actttttctt atttgttgtg
     6241 tcaatccaaa aatttcatct ttagggaaat attttgaaat tgtataaata gaatttgcaa
     6301 gctgtctgct agctatccaa acatctaatt gtttataatc aatcatatta atctcataaa
     6361 attttgcaat agtccctaaa aactaaacac caaaaactag acacgattct ctattcttca
     6421 acctttgctc ttaggctttc ccaaatcaga tatccacata aaatcaggaa catcacaaag
     6481 cctagaattt cggcggagtg cgattcaaac cattctgggt ttacatagtt aatgccgcca
     6541 aagcggagta aggcgctaac tactcccatt agggctccac ctgcaataaa gcccgatgca
     6601 attagtgttc ccctttcttt cctggctctg ttcaatgaat tgtcctttga acgggtggaa
     6661 acaatgtgtg caataattcc tccaaccagt aatggcgtgt taagttgtag gggaataaac
     6721 atacccagcg aaaatgcaag agctggaact ccaatcatgg taaggataag cgaaaggaat
     6781 gcccctgcag cgtaaagtgc ccaaggtgca ggcttgccgc tcatcatggg ttcaattact
     6841 gcagccatgg cattggcttg tggggcaaca agagcgtcct tgccggtaaa accgtatgtt
     6901 ttattaagca ggattattac gcctccaaca gttgctgctg aaacaaatgt cccaaggaac
     6961 ttccaggttt gctgcttata gggcgaggag ccaagccagt aaccaatttt aagatcggtg
     7021 ataaagccac cagccattga gagtgctgta caaaccactc ctccaataat gagcgcagca
     7081 atcattcctg aagttcccga taacccggcc gatgccatta ccaatgaggt gataatcaga
     7141 gtcatgagtg tcatgcccga tacaggattg gtaccaacaa tggcaatggc attggcagca
     7201 acggtggtga acagaaaagc aataaccatt acaataccaa gcgctataag agcatgggta
     7261 atattatgaa ctactccaaa gtagaagaat actaaaagca gaatggtagt gaataggata
     7321 cctccagcaa taatggacat gggaagatcc cttcgggtgc ggagttcctc cttggggata
     7381 gccgagtgct ttccggttaa ctcactaacg gccaggccaa atgctttacg aatgatgccg
     7441 cttgagcgaa taatcccaat tattcccgcc atggcaatac caccaatacc aattgggcga
     7501 acgtagttac tgaaaatctg ctcggcgctc atctgcccaa cggttagtgt aatgttactg
     7561 cctactgcct cagttaaacc gggcgcaaag tagttgatta tgggtatgat aacaaaccac
     7621 ccaacaaacg accctgctgc aattattgcg gtgtacttga ggccaataat ataaccaata
     7681 cccataacgg cagcaccaac attaagttta aataccagct ttgctttctc ggcaagagcc
     7741 tcgccagcag gtatcactcg ggtggtaacc acctcgctcc accaaccaaa tgtggcaata
     7801 atgaagtcgt agataccccc aattagcccg ctaaccacaa gtaaacgagc ttgtttcccc
     7861 cctttctcgc ccgatacaag tacctcagtg gttgctgtgg cctccggaaa aggaaattta
     7921 ccatgcatgt cggccacaaa atatttacgg aatgggatga ggaatagaat tccaagaatt
     7981 cccccaaaca acgatgacag gaatacctga tagaattctg cctccaggtt caggatgtat
     8041 agagctggta atgtaaaaat agcaccagca acaattaccc cagaacttgc gcctatggat
     8101 tggataatta cattctctcc cagtgcccct ttgcgtttag caagggttga tagccctaca
     8161 gcaatgattg caatgggaat tgctgcctca aatacttgac caatttttag cccgagatag
     8221 gcagcggctg ccgaaaaaac aatagccatg attagccccc aaataacact ccaagcagta
     8281 acctcaggca ctgccttgct tggtggcatt actggttgat actcttcgcc ttcgcggagc
     8341 tcagtgtagg cattttctgg aaggcctttg attttttcta caggttcgtg cgacatagtt
     8401 tttatggttt aggtttgaga ttcaaaatat cccaaaccta ctaattttta gtaaatgttt
     8461 aatccaaatt caatgtcgtt tagttaacaa aaatatacat gcctgtcatc ctgcgcgcag
     8521 cgaatgatct cttttcgagc ccatgagatg tttcgctaac gctcaacatg acaatagctt
     8581 caccaacaac gattcacgaa taacgaacaa cgcatctagc cccgtagggg ctaaacattt
     8641 gtaaccctat gtttactccc acttgtattg cgatgcacgc aatgcatata ccaaagagca
     8701 atggtgaggc tttgcatcgc aaaggaaaaa catcgcaaag agtgattttc acgttctttc
     8761 ttgtcttgat acaagaaaga accaaagaaa atcaaggctg aaaagcccga cccgatgggt
     8821 accccgcggg tggaactgcc gcgcgatact attcgccacg cctgtggcgt gactcatttg
     8881 gtatcgctta ctaagcccac cgccacgttc cactcccgac ccattgccgg gtcaggcttt
     8941 tatgcccctt tctatgccat tctttgttgg tttattaatc tcattaaaag agttgaaggt
     9001 acaaagagat gcttgggcta gctcagcatg acaagggagc agaaaggaag atagggtttt
     9061 tgtcatcccg agcaaaggcg agggatctct tcaaaggatt gaagagaaaa gagcaatgag
     9121 atgctacggc gatgtctatc cctaaaaatc gaaactcact acgacttgtg atgtttcgct
     9181 tgcgcgcaac atgacaaata cccttaacta aacgacattg aatccaaatt cgtcaattga
     9241 acttttcaat tgacgaatcg actaacaaaa tctaatcatt ccgagatctc ggaatgaaag
     9301 attttgataa gtcgggatta cctcgataat ttaatagaaa caagttgttt aaatgtttta
     9361 ttgtgtttct attgactaat cttctctaaa aatgtttctg tatccacatt ttaattagcc
     9421 cggttgtatt tgtttgttct tgttcgtttg gtttagccgt tcactttttt actattcctc
     9481 taacttcctc taatttcctc taactccatc atattctgct tattccgatt cattccgatt
     9541 cattcaccta aaattagccc tgttgaattt gttaattcat ggtgctttgg tttaataata
     9601 caaatcgata tgaaaaaaaa ttcttttcgg taacaacttt tttgtacata tttgcaaaac
     9661 aatgaatcag tgttatgata tcgttagata aggcgataag gattgtagaa caaagcgtta
     9721 ccgctttgaa tggacaagag catgtttcat tatccgaaag tctaaaccga gtgttgaggc
     9781 atgatgtggt ttccgatata aatatgccgc cattccgcaa aacggctgtt gatggttacg
     9841 cttgcagggc tgcagatttg gataatcccc ttacagtaat tgaggttatt gcagcaggga
     9901 ctatacccca aagaactata gcccagggca catgctcgaa gataatgacc ggcgcaccca
     9961 tacccaatgg agctgattgt gttatacccg ttgaggatac cgaaatattg tccgatggaa
    10021 ctattcgctt taaaggcagt aaaccaaaat ctaacatttg tgaacttggc gaggacatca
    10081 aaattggtga gtcgccgtta agcaaaggaa cccttattaa gcctcagcat atagccataa
    10141 tggctgcact cggatgtcat tccccgatcg tttctgtaag gcctagagtg tcaattttgc
    10201 caaccggcga tgagcttgtt gagccaactg aaataccaca gggtagcaag attaggaata
    10261 gcaacgggca tcaactaatt gcacaggtaa tttcagcagg tgcaattcca atgtaccatg
    10321 gtatagtagg cgataccgag gaggttaccg aaaaagccat tgctagggct ttggctgaaa
    10381 gcgatgtggt agtgcttaca ggtggagttt caatgggcga ttttgattat gttccaagaa
    10441 ttatggagcg tttgggtgtt aaaatcctat tcgattccat agcagttcaa cccggaaaac
    10501 caactacatt tggagttgct ggcaataaat taatatttgg attgcccggt aatcctgtat
    10561 catcgtttgt acagtttgag ttgctggtaa aacctgcact cctgatgatg atgcaatgca
    10621 atgagcctta cgctaggaca tacaagttgc ccttagcaat tgattattcc cgaaaacgag
    10681 ctgaacgttt agggcttttc cctgtttttg ttaacgatat aggggaggtt gaacctcttg
    10741 agttccatgg ttcagcccat atattttcgt tggcaaaggc tacaggtatt gcagctattc
    10801 cgcttggagt aaaagaactc aaaaaaggag aacttttgga tgttcgacca atttaatagg
    10861 cgcattaatt acctaaggat atcggttacc gataggtgca acctgcggtg tacctactgc
    10921 atgcccgagg agggtattgt ccaaataccc cataaccagg tcttaacctt tgaggaaatt
    10981 gtagaaatag ctcggattgc ggttcagcat ggttttgata aggttcgcat tactggtggc
    11041 gaaccgctag tccgcaaagg aattgtggac ttggtaagga tgcttaaaac agttgagggt
    11101 attaccgatt taggtttgac taccaacgga acgcttttaa aacaatttgc tcagcccctt
    11161 tacgatgccg gactaaaacg cataaatgtt agtctcgata cgttagatcc ccaaaagtat
    11221 aaggctataa caagggttgg cacgctatcc gatgtgctgg agggacttga ggaagccaaa
    11281 aaggtggggt ttaaccccat aaaattaaat tgcgtggtag aaaacaactc taacgaaccc
    11341 gatgcccaag ccgttaaagc atttgctaac tctaatggct ttattgcccg ttttatccca
    11401 aagatgaacc tcgatgatgg tgtctttggc gttgttgagg gtggcgaggg tggaaattgc
    11461 agcacctgta acaggttgcg cttaaccgcc gatggtattc ttaaaccatg cctttttagc
    11521 gacaagggat ataacgtaag ggagttaggc atacagcaag caattgaaat ggctataaaa
    11581 aataaaccag cttgcggagt tgcaaaccgt agcggtgagt tttataacat aggaggctga
    11641 gatggaacta tcgcacaccg ataaaaatgg caaggccaat atggttgatg ttggtcataa
    11701 acccaatcaa atacgcgagg ctagcgctac tggctttatt cgtttactac cccaaacggt
    11761 aaagctgatc cgtgaaaacc aaatgaaaaa gggtgatgtt ttaaccattg cggagattgc
    11821 aggcatacag gctgctaagc gaacccctga actaattccg ctttgccata cactcatgct
    11881 tactaaggtt gatgttaaag ctaccctttg cgaaaatggg gttgaggtta aaagttacgc
    11941 caagtgcatt gggcaaaccg gtgtggagat ggaagcacta acagcagcaa ccgtggcgct
    12001 ccttaccatt tacgacatgt gcaaggctgt tgacaagcaa atggttattg aacgcgttga
    12061 actaattgag aaagctaaaa aggatattgt ttagctatgg agcaaactaa tcccattact
    12121 gtaaaggttg tatcggtaaa tatctccgaa aaaaaaggaa caactaagca tgcagttgat
    12181 cgtattgagc taaactcgct tggtgttaaa ggcgatgcac atgctggtgc ctggcatcgt
    12241 caggtgagca tgctcggcgt tgagagtatc gataagttta caaaggctag cggcctgact
    12301 gtaaaatatg gcgattttgc cgaaaatatc accactcaag gtcttgaact ttacaggaca
    12361 aagccaggcg accgttttgt gggtgctggt gttgaacttg aggtgacaca aattggcaaa
    12421 tcgtgccatg gcgatgggtg cgccatctac cgtcaggtgg gtaactgtgt gatgcctaag
    12481 gaggggatat ttgttcgggt actgaaagga ggtcccttaa aagcaggcga tgagcttagc
    12541 ttttacccaa aggtttacag ggtgaaagta attactttaa gcgacagggc aagtagaggc
    12601 gagtatgagg accttagcgg tcctgaggtt gctaaacttg tttccgattt ctttaactcc
    12661 gtatcctggc agttagaggt ggaaactgaa attattcccg acgacagaga tttgctagag
    12721 aacagactga ttgaagcacg aaactctaaa tacgacatgg ttttaacaac tggcggaact
    12781 ggaattggcc cgcgcgatat tactcccgat gttgtaaaac cattgctcga taaagaaatt
    12841 ccagggatta tggagcacat ccgcttaaag tatggggccg aaaagccaaa cgctttgctt
    12901 agtagaggcg ttgctgggtt aatgggaact acatttgttt acacattacc cggaagcgtt
    12961 aaggctgtaa gggaatacat gggtgagatt tcaaaggtgc aaaagcattt gtttttgatg
    13021 ctgatgggga ttgatagtca ttaaaggata agggttaaag gttaaaggat aaagagcctg
    13081 tcccgcatta gcgggaataa aggttaaagg ataaaagtcc cgcctcgctc tacgggggta
    13141 aagagccagt cccacgaagc gaaattcaaa gttcagggtt aaggttttaa ggccatgctt
    13201 tctggaatct aacacaaaat caaccagaat aaaaaaagga gcctttggct ccttttcctt
    13261 tattacaaat aggttcaatt actcaacacc taaaacatcc ttgaatgcct ttttaaaggt
    13321 gtccttaggt aatgccccca tagccatttg tggctgatca ttaagtggaa taaagagtaa
    13381 cgatggaatg cttcgaattc caaaaacggc agcaagttcc tgctcttcct cggtatctac
    13441 cttgtaaata ttgagtttgc cctcatattc cttggcaagt tcttccagaa taggtgctac
    13501 cattttgcag ggaccacacc aatcggcata gaaatcgatg atggcaggct tgtcgccctc
    13561 aaacttccac tcgttattct tttcgtaatc aaatactttc tgcttgaatg tttctgctgt
    13621 taagtgctca atcatagtgt tactggttta tttattaata aatcaaatta tttacatatt
    13681 tcgatgcaaa agtaatacca aaacatttaa tggcataatt ttgttgaata ttttaagctg
    13741 atatatgtca ttttatcatt gcgatgtttt aaacaaagca tttttactat aaaacaaaaa
    13801 tttaggctaa aagtttgata agttgtaatt ttgtcaggca taacataaca atatgaagga
    13861 atacctaaaa agcaacatat ttagagtaat atcggatgtg gcagagattg agggggttga
    13921 tgcttacgtt attggaggct ttgtgaggga tattttactg aaccgaccat cgaaggatat
    13981 tgatatagtt gttgtaggca gcggagtaga ttttgcacgc aaggtggctc aaaggcttag
    14041 ggcacggaag gtaacctatt tcaaaaactt tggaacagca cagctaaagc acaacgagtg
    14101 ggatattgag tttgtaggcg cccgaaagga atcgtacaga ctcgactccc gaaagccaat
    14161 agttgaaaac ggttcgcttg aggacgatca gaaaaggcgc gattttacca tgaatgccct
    14221 tgcaataggg ctgaacaaac gaaactttgg tgagctaatt gatccgttta acggtttgca
    14281 ggatttaaaa agtaaaacaa tacgcacacc gctcgacccc gccaccacct tttccgacga
    14341 ccctttgcgt atgctcaggg caatcaggtt tgcaacacag ctaaactttg atattgagct
    14401 caaaacccta gatgctattc gtgaaatgag ctcgcgaatt actattgttt ccattgagcg
    14461 ggttgtggac gaaataaata agatgatggc ttcaccacgg ccatcggtag ggtggttact
    14521 tcttgaacaa acagggctat tgcaacatat ttttccggaa ctggcggcaa tgaagggtgt
    14581 ggaagcgcgt aatggagtat cgcacaagga taacttttac catacgcttg aggtgttgga
    14641 taacctgtcg ttgaaaagcg ataacctttg gttgcggtgg gctgcattgc ttcacgacat
    14701 tggcaagcca aaaacaaaac gctttgtggc gggtcagggt tggacttttc atggacacga
    14761 atttttgggc gcaaaaatga tccctgaaat atttcgtaag ctcaagttgc cccttaacga
    14821 taagatgaaa tatgttcaaa aaatggttct gcttcatctt agaccaatcg ttttatcgca
    14881 ggaggttgtt accgattcgg ctgttcgccg tctgctattt gatgcaggcg acgatattga
    14941 tgacctgatg atgctttgcg aagccgatat cacctcaaag aacgatatta ccgttcggaa
    15001 acatttggca aattttagac ttgtaaggaa gaagcttaag gaggtggagg aaaaagattc
    15061 cattcggaac tttcagccac ccgttactgg cgaaatgatt atggaaacct ttaacctgaa
    15121 gccctgtaag gaagtaggac tcattaaaaa taggattaag gatgctattc tggatggcga
    15181 aatccgtaac gatttttttg aagcctatac ccttatgcta aacgctggta aagagttggg
    15241 tttgacagcg ataaatatta accctcaata catctcattg acaaataaat gattattact
    15301 tttattaata aaaggttatg gtagcaaccc aaaaaatcag cataaaacac atcgtggcat
    15361 ttgttctctt cctactatcc tacatcatca tggttataat aatgccatgt gaaggtagat
    15421 ttagctatga attccaaaaa ggaagacctt ggatgcacga ggatctttat gcccctttcg
    15481 attttccagt ttataaaact gagcaagagc tttttgctga gaaaagcaag ctgctaaagg
    15541 agttcaagtc cttttaccgg tacgattcta ccattcaccc tcagcaactg gaggggtttc
    15601 gttccagttt cgacttggca tttgataaat ttattaatag ccagaatgtt tcaaaaacat
    15661 caagggcaaa gtacctgaaa gtaaaaccgg aactagaaca gtttgttgcc gataatatta
    15721 gccagattta tctaaaagga attatcaatc aggatgagag cgccatctca aacagcatgc
    15781 tcaaacaggg attggctgtt gttatcaata atgtggccta cgataagtgg ttcgatgagg
    15841 tttatac
//
LOCUS       DAOSAL010000061        15768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_2562_length_15768_cov_46.981289, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000061 DAOSAL010000000
VERSION     DAOSAL010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 15768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15768
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2562_length_15768_cov_46.981289"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="PK049_08670"
     CDS             <1..519
                     /locus_tag="PK049_08670"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOK61672.1"
                     /translation="GIILYPTDTIWGLGCDATNEQSVRRLMDIKGRVNGKGFVILADI
                     PQRIPSYVDEVPDMAWELIGLADKPLTIVFDKGKNLAPSVYGQSGSVAIRVTQSKFCQ
                     QLISRFGRPIVSTSANFCGTPFPKNFDAISPEIVSRVDYVVPGNIDTESTGKPSSIIA
                     LGKGNQVSIIRE"
     gene            complement(536..1330)
                     /gene="rnc"
                     /locus_tag="PK049_08675"
     CDS             complement(536..1330)
                     /gene="rnc"
                     /locus_tag="PK049_08675"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOK61673.1"
                     /translation="MLKFLSKPIRRLRLTAADKEFYQNLKKILGFAPDNLDVYKLALV
                     HRSASLIISPGQKVDNERLEYLGDAILDAIVADYLFAEFPNSDEGFLTKMRAKIVSRN
                     NMNQLAISIGLPDVVKVNNGALNHQKYIYGNALEAIVGAMFIDKGFKFTSDVVVNNFL
                     RRHINLEELESIEYDYKSRILEIAQKLHIKVAFDTHDYGDNKQKPQFEAILIWNGKEI
                     GRGLGQSKKEAEQKASMQGIELAEGELQSEKQKEDLPNEDPSNEQV"
     gene            complement(1340..2587)
                     /gene="fabF"
                     /locus_tag="PK049_08680"
     CDS             complement(1340..2587)
                     /gene="fabF"
                     /locus_tag="PK049_08680"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOK61674.1"
                     /translation="MEMKRVVVTGIGTINPLGNNIQEYWTNLENGVSGCELITSFDTS
                     KFKTKFACMVKNFDPNNYFDRKEVKKLDPYTQFALVAVEEAMKDSGINPEAIDLDMAG
                     VIWASGIGGLQTLTEEMRGYFGGDGTPRFSPFFIPRMIADIAAGHISMKYGFRGPNFS
                     TVSACASATNGIICALDVIRTGKANVVITGGSEASVNEIGVAGFNALQALSTNNEEYK
                     TASRPFDATRDGFVMGEGAGCLILEEYEHAKARGAKIYCELVGGGMSADAYHLTAPHP
                     EGLGARKVMENALRDAGMKPEDIDYINVHGTATPLGDIAETKAIIQVFGEHAYKLNIS
                     STKSMTGHLLGAAGAIEALAAVMAVYKDVVPPTINLKEVDPEIDSKLNLTPNVAQKRT
                     VRAALSNTFGFGGHNASIIVKKM"
     gene            complement(2641..2877)
                     /locus_tag="PK049_08685"
     CDS             complement(2641..2877)
                     /locus_tag="PK049_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004347843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOK61675.1"
                     /translation="MSDIATRVKSIIVDKLGVDENEVTPEASFTNDLGADSLDTVELI
                     MEFEKEFNLSIPDEEAEKISTVGDAIKYIEEHSK"
     assembly_gap    3036..3135
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3136..3318
                     /locus_tag="PK049_08690"
     CDS             <3136..3318
                     /locus_tag="PK049_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016272085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HOK61676.1"
                     /translation="ESGITIHYVNEHYDEGDVIFQASCEVLPTDTPETLAQRVHRLEW
                     EYYPKVIEHLIQGKCG"
     gene            complement(3315..4004)
                     /locus_tag="PK049_08695"
     CDS             complement(3315..4004)
                     /locus_tag="PK049_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61677.1"
                     /translation="MAFPKMLSGLIRLVALALILQACKIAYIPNTFNSPLLRNKGDAQ
                     LNIWAGSAGIEAQTAVAVTNSIGLMVNGQLQQNKKTENDVEVTEQRKYVEGAIGYTER
                     FSDMGIFEFYAGGGVGQVPAKFRSITWDGPQTIAYNRWFIQPALGFCNDWLDFSVVSR
                     LSLVDIGPQKNWFYEPGIVGKIGYKRLRFVGCFGLSIPLREHDKRYWDNLPFTISIGL
                     HLNFGKRIVEN"
     gene            4276..5007
                     /locus_tag="PK049_08700"
     CDS             4276..5007
                     /locus_tag="PK049_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61678.1"
                     /translation="MPYRRLPNTDSARIRALKAAYKMGKNTHPQDLAFSQATFSKVEL
                     FINSFEKAIAHYKAAYDTQVERSKDYQQLVKKARLYLSHFIQVVNMAIARGELQPTVR
                     ELYGMDIDESKIPSLNTEKDLIEWGKKIIEGEMARTRRGMSPITNPTIAVVKVRYDQF
                     LEAYNYQKVLQHNTQRMLNELIELRKKADDIILSIWNEVEEHYKDLPDEERREKASAY
                     GLVYVYRKNELKGLSLFDSAIPRLF"
     gene            complement(4969..5892)
                     /locus_tag="PK049_08705"
     CDS             complement(4969..5892)
                     /locus_tag="PK049_08705"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOK61679.1"
                     /translation="MEHGILESKSSVGKMLIFGAIILFSSFIFSLIGFLLLGILFGAS
                     GVQVALGESLTNLNALKLMQILQTTGIFIVPAILSASLFSKKPWHYLGFQRVKTDTIF
                     KTLLIVLISLPAINLLASVNQLIPLSDWMIELEKKAEALTKAFLITDSFWVFLVNIFM
                     VAILPAIGEELIFRGILQRRLVELTRSRWTGIIIAAAVFSGIHMQFQGFIPRFALGVA
                     FGYLLEVTGSIWVPIFAHFFNNAIAATGYMLIGTGTVDERIEDIGGLSDLWLLGIISL
                     IATILLVVKLKREIERNRQLEQPRDSAIEQR"
     gene            5987..7015
                     /gene="dusB"
                     /locus_tag="PK049_08710"
     CDS             5987..7015
                     /gene="dusB"
                     /locus_tag="PK049_08710"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOK61680.1"
                     /translation="MKIGNVDLGEKPVLLAPMEDVTDPSFRFMCKHFGADMMYTEFIN
                     ADGLIRDAGSSVAKLTIYDYERPIGIQLYGHIIESMVEAARVATEANPDVIDINFGCP
                     VKKIANRGAGSGMMRDVPKMVEMTRRIVEATHLPVTVKTRLGWDDQSKNIVEIAERLQ
                     DVGIKAITIHGRTRAQLYTGKADWTLIGEVKSNPRMHIPVIGNGDIDSPIRAKEMFTR
                     YGVDGIMIGRATYGRPWIFAEVKHYLKTGELMKPLSTTEKVEIARLHFEHSLQVKGAK
                     TGMLEMRRHFGSYFKGLPHFKDIRLKLLTTLNPDEVYSLLNLIAERYGDYVVDESINP
                     SPWADKQD"
     gene            complement(7012..9783)
                     /gene="polA"
                     /locus_tag="PK049_08715"
     CDS             complement(7012..9783)
                     /gene="polA"
                     /locus_tag="PK049_08715"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOK61681.1"
                     /translation="MKKRLLLLDAYALIYRAYYAFINRPMRNSKGMNTSAIYGFIRAT
                     LDAIKKFNPTHVAVAFDVSGKTFRNDIYPDYKAQRDETPEDIRASVPVIKNLLNALNI
                     PIIEKEGFEADDVIGTLATKANPKEFEVIMMTPDKDYGQLLSENIIIAKPGRSGNEME
                     IVTAEQFCKDYDIQDPKQFIDILALWGDASDNIKGVNKVGEKTAAKLISQFGSIDNLL
                     QNLDKLSPAQRENFEAALNIIPLNRKLVSIVTNIDLNVDIENDLAVKPPKTNELVPIL
                     EELEFRSMLKEFTATPKVEKPQYVQGSLFDMPATETPKPAINLKTINDFEVDYRIAQT
                     TEERRILIKELENSSEFAFDTETTGLDPISVELVGLSFAISPRKAWWVPVPSDQNQAK
                     EVVKEFANVFGTETIAKVGQNIKFDMQVLLSYGIDIKGHLYDTMLAHYLLEPDSRHNL
                     DYLSEIYLSYKPISIEELIGKKGSKQGSMRNVEATLITRYACEDADLALQLKHILFSK
                     LEEQGLTNLYFALEAPLVEVLTYMERNGVSLDTKSLKDYGEILSAQLVELESEIRQMA
                     GVPDLNISSPKQLGEVLFEKLKISSDAKLTKTRQYSTNEEELQKYIDAHPIVPKILEF
                     RGIKKLLSTYIETLPTLVNPKTGKIHTSFNQAVASTGRLSSNNPNLQNIPIRDENGRE
                     IRKAFIPSPGDYLLLSADYSQIELRLMAHMSTDPAMLEAFEKGQDIHAATAAKIFRIP
                     LNEVTADQRRKAKTANFGMIYGISAFGLSQRLGISRTEAKELIDGYFTTYKGVKEYME
                     KCVSQARERGWVETIFGRKRYLPDINSNNQVVRGLAERNAINAPIQGSAADIIKMAMI
                     RIHDEFKSGKLKSKMIIQVHDELVFDVYRPELDEVKAIVKKCMEGAAALSVPLEVEIG
                     TGSNWLEAH"
     gene            complement(9799..10245)
                     /locus_tag="PK049_08720"
     CDS             complement(9799..10245)
                     /locus_tag="PK049_08720"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HOK61682.1"
                     /translation="MPKIRLTKEFRFEMAHALEGYDGLCRHMHGHSYILAVTVIGEPI
                     DKQGHPKLGMVMDFGDLKRIVNPLIVDRFDHAVAVMANTPTHTKLREAGFNRIEALPF
                     QPTCENMIHYFAEIIKANLPVNVTLHHLKLNETATSYAEWYATDNL"
     assembly_gap    10275..10374
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10386..10748)
                     /locus_tag="PK049_08725"
     CDS             complement(10386..10748)
                     /locus_tag="PK049_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61683.1"
                     /translation="MDSLTGKWTFTEEFECGTDKGFAFLEQKGDLIVGYLEYEECIED
                     EEPFMVRQIVEGNVQGHTIVLKGLEVMHPDGTPILNYNLDVLEGTYTHEKKIVGHSYD
                     SEDICGVFVMTRVVGDEL"
     gene            10851..11699
                     /gene="nfo"
                     /locus_tag="PK049_08730"
     CDS             10851..11699
                     /gene="nfo"
                     /locus_tag="PK049_08730"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014775177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HOK61684.1"
                     /translation="MKFIGAHVSAAGGVENAPVNAHSIGATAFALFTKNQRQWEAPPL
                     TQKSIDAFKANCEKYGYTPSQILPHDSYLINLGNPDRDALEKSRNAFLDEMVRCQQLG
                     LDRLNFHPGSHLNKISEEESLLLIADSINMALERTQGVTAVIENTSGQGSNLGYTFDQ
                     IRYIIDHVNDQSRVGVCIDTCHAFTAGYDISTPEAFAKTFSHFDRVIGFKFLKGIHLN
                     DTKKGLGSRVDRHENVGDGLLGIEPFRLIMTDPRFNGIPIILETPDESRWAEEIKMLM
                     KFANAK"
     gene            11765..12976
                     /locus_tag="PK049_08735"
     CDS             11765..12976
                     /locus_tag="PK049_08735"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOK61685.1"
                     /translation="MEIQALYPEFEEALVNELKQLPVMEIDAGMEIPMHGNVASHIPL
                     GVEGSVRVFGRDSEGHEADLYEVKPGVACIVSFTSAMIDTPAITYAKAKQKSKIIPVP
                     KELSNTWFVKYTSWRQYIIGLYQNRLDELIKQNERVVAQKEMIEIQKNQIIQSINYAR
                     NIQKAVLPVESQLKDFFADYFILYIPKDIVSGDFYWTYYQNDKVFFAAADATGHGVPG
                     AFMSMLGISLMNEICYNFSGNAADFLNTMRNKTKEALKQTSFDSGPRDGFDLQLCIFH
                     TRQGIVEYAGANNNLLLSRNNQIFEFNADKMPVGVAYKEEKPFTNHSISVQDNDIFYL
                     FSDGYKDQFGGTEQQKFSKKRFYNLLSQISSKPLTEQKEILEKKFHDWKGSNTQVDDV
                     LVMGIKIRAKE"
     gene            complement(13082..14059)
                     /locus_tag="PK049_08740"
     CDS             complement(13082..14059)
                     /locus_tag="PK049_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HOK61686.1"
                     /translation="MDSSKPITSFIEKHYLHFNSATLVDAAKAYKAHIESGKKMMITL
                     AGAMSTAELGISLAEMIRQDKVQIISCTGANLEEDLMNLVAHNHYKRVPHYRDLTPQQ
                     EWELLQNGLNRVTDTCIPDEEAFRRLQSHLFDVWKEAQDKGERYFPHEYMYKMLKSGV
                     LEQYYQIDPKNSWMIAAAEKNLPIVVPGWEDSSMGNIFASYCITGELKPSIVKSGIEY
                     MMFLADWYTNNAGTEGVGFFQIGGGIAGDFPICVVPMLHQDLERTGVPFWSYFCQISD
                     STTSYGSYSGAVPNEKITWGKLNIDTPKFIIESDATIVAPLIFAYVLGW"
     gene            complement(14097..14537)
                     /gene="rpiB"
                     /locus_tag="PK049_08745"
     CDS             complement(14097..14537)
                     /gene="rpiB"
                     /locus_tag="PK049_08745"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019149741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOK61687.1"
                     /translation="MSKPTLAIASDHAGYSTKEELKKYLAELGYNVWDFGTHSDDSVD
                     YPDFAHPLATAIEKGDYQLGILLCGSGNGVSITANKHQGVRSALCWLPEIATLARQHN
                     DANVCAIPARFVSVSEAKQIVKAFLDAKFEGGRHTRRVEKIPVK"
     gene            14696..>15768
                     /gene="hemW"
                     /locus_tag="PK049_08750"
     CDS             14696..>15768
                     /gene="hemW"
                     /locus_tag="PK049_08750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOK61688.1"
                     /translation="MAGIYIHVPFCRKKCSYCDFYSVGEAKMDNHFTDFVLKELSLRN
                     PLIADRIVKTIYFGGGTPSLLPAGNIKRIISGLGDFFGVDPNAEITIEVNPDDVTLEL
                     AEQYQAIGVNRVSIGIQSFNNDELIFLGRRHDVGKAIEAIRIFRKASIANISIDLIYG
                     LPHSTLKSWEHSLNVAFGLDIQHLSCYHLTYEEGTPLTRRVAKGTVIPLDEELSKAQF
                     DLLCEMSNNYDFIHYEISNLAKPGYISKHNSGYWLGEEYLGLGPSAHSFNRSMRWWNP
                     SSITGWEKEVDSANFPKNMEAIGEVTRFNELLITRLRTMWGVNLEEVANGFSPEIYNR
                     LRKKIIPFTASGKMVMENNILKIP"
BASE COUNT         4371 a         3509 c         3331 g         4357 t
ORIGIN      
        1 ggcattatcc tttaccccac cgataccatt tggggtttgg gttgcgatgc tactaacgaa
       61 caatctgtaa gaaggttaat ggatattaag gggagggtta atgggaaagg gtttgttatt
      121 ttagctgata ttccccaaag aattccttcc tatgttgatg aagttcccga tatggcctgg
      181 gaactaatcg gtttagccga taagccctta actatagttt ttgataaagg aaaaaacctc
      241 gccccatcgg tttacggaca aagcggcagt gttgccataa gggtaaccca aagtaagttt
      301 tgccagcagc ttatttcaag gtttggccgc ccaatagtat caacctcagc taatttttgc
      361 ggcacaccat tcccaaaaaa tttcgatgcc atttccccgg aaatagtatc gagggttgat
      421 tatgtggttc cggggaatat cgataccgaa tcaacaggga aaccttcgtc gataatagcc
      481 ctgggcaagg gcaatcaggt tagtattata cgggaataag caaaggccaa aaaacttaaa
      541 cttgctcatt tgagggatcc tcgttcggga ggtcctcctt ttgtttttcg ctttgtaatt
      601 cgccctcagc taattcaatt ccttgcattg aggctttctg ttccgcttcc tttttcgatt
      661 ggcctaggcc acggccaatc tcttttccgt tccagattag gattgcctca aactgtggtt
      721 tttgcttatt atcgccgtaa tcgtgggtat caaaggcaac cttaatgtgc agcttttgcg
      781 caatttccag aattcggctc ttgtaatcgt attcaatgct ttccagctct tcaaggttga
      841 tatggcgccg taggaagttg tttaccacca catcgctggt aaacttaaat cccttatcga
      901 taaacatggc gccaactatg gcttccaggg cattaccgta aatatacttt tggtggttaa
      961 gggcaccatt atttaccttg accacgtcag gtagcccaat actgattgca agctggttca
     1021 tattgttgcg gctaacaatc tttgcccgca ttttagtaag gaaaccctcg tcgctattgg
     1081 ggaattcagc aaacaggtag tcggccacaa tggcatcgag tatggcatcg ccaaggtatt
     1141 ccagcctttc attatcaacc ttttggccag gcgatataat aagagaagct gacctgtgaa
     1201 ccagcgccag cttgtaaaca tcaagattat cgggggcaaa ccccaaaatt tttttaagat
     1261 tctgataaaa ttctttatcg gcggccgtta agcgtaaccg acgtatgggt ttacttaaga
     1321 attttaacac gatgaaaaac tacatctttt ttacgatgat ggatgcgttg tgtccaccaa
     1381 aaccaaaggt gttacttaaa gcagcacgaa cagttcgctt ttgagcaaca tttggcgtta
     1441 ggttaagctt gctgtcaatt tcaggatcaa cctctttaag gttgatggtt ggtggtacca
     1501 catctttata aacggccata actgcagcaa gagcctcaat ggcaccggca gcgccaagca
     1561 ggtgaccggt catcgatttt gttgagctga tatttagctt gtaggcatgc tcaccaaaaa
     1621 cttggataat ggcttttgtt tcagcaatat cgccaagtgg agtggctgta ccatgaacgt
     1681 taatgtaatc gatatcctcg ggtttcatgc cggcatcgcg taaggcattt tccataacct
     1741 tacgggctcc aagcccctcg gggtgaggtg ctgtcaggtg gtatgcatcg gccgacatgc
     1801 cgccgcctac cagctcacag taaattttag ccccacgggc tttagcatgc tcatactcct
     1861 ctaggataag gcaacctgca ccttcgccca tcacaaaacc atcgcgggta gcatcgaacg
     1921 gacgcgaggc ggttttgtat tcctcgttgt tagttgatag tgcctgaagc gcattgaatc
     1981 cagccacgcc aatctcgtta actgaggcct cagaacctcc ggttataaca acgtttgctt
     2041 taccggttcg gattacatcg agggcacaaa taataccgtt ggttgccgat gcgcaggccg
     2101 atacggttga gaagtttggt ccacggaaac cgtacttcat ggagatatgc ccggctgcta
     2161 tgtcggcaat catcctagga atgaaaaagg ggctaaatcg gggtgttcca tcaccaccga
     2221 aatagcctct catttcctcg gtaagggttt gtaaaccccc aatacccgac gcccaaatta
     2281 ctccagccat gtcgaggtca atggcctcgg ggttaatgcc tgaatccttc atggcttctt
     2341 caactgccac cagcgcaaat tgtgtgtaag gatccaactt tttaacctct ttgcggtcga
     2401 agtagttatt tgggtcaaaa ttctttacca tgcaggcaaa tttggttttg aatttcgacg
     2461 tgtcgaaact tgtgataagt tcacaaccac taactccatt ctcaaggttt gtccagtatt
     2521 cctgaatgtt attcccaagg gggttaatgg ttccaatacc tgttacaaca acacgtttca
     2581 tttccataaa agtgaattta agtttaaaac caaaatttgc cttgccggtt aataagttaa
     2641 ttacttgctg tgctcttcaa tgtacttgat agcatcacca acggtgctga tcttttcagc
     2701 ttcctcatcg ggaatagaaa gattgaattc cttttcgaat tccataatca gctcaacggt
     2761 gtcaagtgaa tcggcaccaa gatcgttggt aaaactagct tcaggagtta cttcgttctc
     2821 atctactcca agcttgtcaa caatgatcga cttaactcta gttgcaatgt ctgacatagt
     2881 tttaaaattt agttagtaaa ttgagttttc aaatcacaaa gaaaaacata ttttcacctg
     2941 aaaacaaatc agatgtcaaa aacgtggcaa aagtaatttt tttgttcata tttttgacgc
     3001 ttgttaacaa tttttttttg acagtcgttt aaattnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnngaatc ggggataacc attcactatg tgaacgaaca ttacgatgaa
     3181 ggcgatgtaa tttttcaggc atcctgcgag gttttaccaa ccgatacacc cgaaacccta
     3241 gcgcaaaggg tgcataggct tgaatgggaa tactacccaa aagtgattga acacctgata
     3301 cagggaaaat gtggttagtt ttcaacgatt cgcttcccaa aattcaggtg taaacctatg
     3361 gatatggtaa agggaagatt atcccaatac cgcttatcgt gttcccggag cggaattgat
     3421 agtccaaaac atccaacaaa tcttaaccgc ttataaccaa ttttgcctac aattccgggt
     3481 tcgtagaacc aatttttctg aggaccaata tcaaccagcg ataatcgact taccacgcta
     3541 aagtcgagcc aatcgttaca aaagcccaaa gcgggctgaa taaaccatcg gttataggca
     3601 atagtctgag ggccatccca tgtaatactt cggaactttg caggtacctg acctacaccg
     3661 cccccagcat aaaactcaaa tatacccatg tcgctaaaac gttcggtata gccaatggct
     3721 ccttcaacat acttcctctg ctcggtaacc tcaacatcat tctccgtttt tttattctgt
     3781 tgaagttgtc cgtttaccat cagtccgata ctattggtaa ctgctactgc tgtttgggct
     3841 tcaatacctg ctgagccagc ccaaatattg agttgagcat cgcctttgtt gcgaagtaaa
     3901 gggctattga aggtatttgg tatatacgca attttacaag cttgaagtat tagggctaaa
     3961 gctactaacc ttattaaacc cgaaagcatt tttggaaaag ccattacttt aaactttgtg
     4021 aacaagttaa cctattgctt gatgtttgtc aataccttac cctataattt ttacaatgaa
     4081 gtagttctaa aaatttctag ttaaaaaata tcttgataaa aagcccataa aatgaggctt
     4141 tcaaagtaag tcgctaattt ttagcaaaat tcccttaaaa aaattttgcg aatagagttg
     4201 attttttgaa ttcttaactt atattcgcaa gtgataagcc tttatcgttc agagcaaaac
     4261 aaaattgata gtgctatgcc ctaccgccga ttacccaaca ccgatagcgc tcgtattcgc
     4321 gcgctaaaag ctgcctataa aatgggcaaa aatactcatc cgcaggattt ggccttttcg
     4381 caggcaacat tctcaaaggt tgaactcttc attaactcat ttgagaaggc tatagcacac
     4441 tacaaagcgg catacgatac tcaggttgaa cgcagcaagg attaccagca actggtaaaa
     4501 aaagcccggc tttacctatc gcactttatc caagtagtta atatggccat tgcccggggt
     4561 gaacttcagc ctacagttcg cgaactctat ggcatggata tagatgaatc gaagataccc
     4621 tcactcaaca ccgaaaagga cttaattgaa tggggtaaaa agataattga aggtgaaatg
     4681 gcgcgcaccc gcaggggaat gtcaccaatt accaacccca ccattgctgt tgttaaggtg
     4741 cgctacgacc agttccttga agcctataac taccaaaaag tgctccaaca caatacccag
     4801 cgaatgctaa acgaactcat cgaactgcga aaaaaagccg acgatataat tctcagcatc
     4861 tggaacgaag tggaagagca ctacaaggac cttcccgatg aggaacgaag ggaaaaggca
     4921 agtgcctatg gtcttgttta cgtttaccga aaaaatgaac tcaaggggct atcgttgttc
     4981 gatagcgcta tccctcggct gttctaattg acggtttcgt tcaatctccc tcttaagctt
     5041 aaccacaagc agaatggttg caattaaact tataataccc agcaaccaaa ggtcgcttag
     5101 tccgccaata tcctctattc tttcatcaac agtcccagtc ccaattagca tataaccggt
     5161 tgctgctatt gcattgttaa agaaatgcgc aaaaatggga acccagatgc tacctgttac
     5221 ctcaagcagg tatccaaacg ctacccccag cgcaaacctt ggaataaatc cttgaaactg
     5281 catatgaatg ccactaaata ctgctgctgc aattattata ccagtccagc gcgaacgcgt
     5341 tagctcaaca agcctccttt gaagaatccc ccggaaaatg agctcctccc ctatagcagg
     5401 aagaatagcc accatgaaaa tattaaccag aaaaacccag aaactatcgg ttatcagaaa
     5461 tgcctttgta agagcctctg ctttcttttc aagttcaatc atccaatcgc tcaaaggtat
     5521 aagctgattt accgatgcca aaaggtttat ggctggaagg gatattaaaa caatcaacag
     5581 tgttttaaaa atagtgtcgg tcttaaccct ctgaaagcct aagtaatgcc atggcttttt
     5641 ggagaaaaga gatgccgaaa gaatggctgg aacaatgaaa atgccagtgg tttgaaggat
     5701 ttgcattaac tttagggcat ttaaattggt taggctctca cctagcgcta cctgcacccc
     5761 cgatgcacca aacagtatcc ctaaaagtaa aaatcctatc aacgaaaaaa taaatgagct
     5821 gaaaagaata attgctccaa aaatcaacat ctttcctaca cttgattttg attctaaaat
     5881 tccatgttcc ataaactata aatttctaca aaagtaattc atatcagctt tgaacaaagc
     5941 ctttaaccgt attcttttga tatttttgca tcgcaaaacc taaagaatga aaatagggaa
     6001 tgttgattta ggcgaaaagc cggtattgtt ggcacccatg gaggatgtta ctgacccatc
     6061 gttccgcttc atgtgcaagc actttggtgc cgatatgatg tatactgagt ttattaatgc
     6121 cgatgggctt atccgcgatg cagggagcag tgtagcaaaa ctaaccattt acgattacga
     6181 acgacccatt ggcattcagc tttacggcca tataattgag agcatggttg aggctgcccg
     6241 ggttgccacc gaggcaaacc ccgatgttat tgatataaac tttggatgcc cggttaaaaa
     6301 gattgccaac cgcggcgcag gctcaggtat gatgcgcgat gttcccaaaa tggttgagat
     6361 gacccgccga attgtggagg ctacccattt gccagttaca gttaaaaccc gcttgggttg
     6421 ggacgaccaa tcgaaaaata ttgttgaaat tgctgaacgc cttcaggatg ttgggataaa
     6481 ggccattacc attcatggcc gcacccgcgc tcaactttat accggcaaag ccgattggac
     6541 actcattggt gaggttaaga gtaatcctcg gatgcacatt cccgttatag gcaacggcga
     6601 tattgatagt ccaattaggg ccaaggagat gtttacccgt tacggggttg atggaattat
     6661 gattgggcgc gccacctatg gtcgtccatg gatttttgct gaggtgaagc attaccttaa
     6721 aaccggtgag cttatgaaac ccctctctac cactgaaaag gttgaaattg cccgcttgca
     6781 ctttgagcat tcgctgcagg ttaaaggagc caaaaccgga atgctcgaaa tgcgaaggca
     6841 ttttggctca tactttaaag gattaccaca cttcaaggat atcaggctaa aactactaac
     6901 cacccttaac cccgatgagg tttactcact cctaaactta atagctgaac gttatggcga
     6961 ctatgtggtt gacgagtcca ttaatccctc gccctgggcc gacaagcaag actagtgcgc
     7021 ctcaagccag ttgctgccag taccaatctc cacctccagc ggaacgctaa gtgcggctgc
     7081 accctccatg catttcttta ctattgcttt tacctcatct agctctgggc ggtaaacatc
     7141 gaataccaac tcgtcgtgaa cctgaattat cattttggac tttagcttgc cactcttgaa
     7201 ctcgtcgtgt attctaatca tcgccatttt gatgatatcg gcggccgagc cctgaatggg
     7261 agcattaatt gcgttccgct cagcaagccc acgaaccacc tggttgtttg agttgatatc
     7321 gggcaggtag cgcttacgtc caaaaatagt ttcaacccat ccccgctcac gagcctgcga
     7381 aacgcatttt tccatgtact ctttcactcc cttgtaggtt gtgaagtagc catcaatcag
     7441 ctcctttgct tcggttcttg aaatacctaa ccgctgcgat aagccaaacg ctgatatgcc
     7501 gtatatcatt ccaaagttgg ctgtcttggc ctttcggcgt tggtcagcag taacctcatt
     7561 caggggtatc ctgaatattt ttgcagcggt tgcagcgtga atatcctgcc ccttttcaaa
     7621 agcctctagc atggcggggt cggtactcat atgcgccata aggcgcagct ctatctggga
     7681 gtagtcggct gatagtagca ggtaatcgcc tggcgatgga ataaaagcct tccgaatctc
     7741 acgtccgttc tcatcgcgta tgggaatatt ttgcaggtta ggattgttag agcttaacct
     7801 tccggttgaa gctaccgcct gattgaacga agtgtgtatt ttccctgttt ttggatttac
     7861 cagcgtgggt agggtttcga tataggttga aagcaatttc tttatgcctc taaactccaa
     7921 tattttggga acaataggat gggcatcgat atacttctgg agttcctcct cgttggttga
     7981 gtactgcctg gttttggtaa gcttggcatc ggacgatatc ttaagctttt caaacagaac
     8041 ctcgccaagc tgtttgggcg atgagatatt caaatcgggc actccggcca tttggcgtat
     8101 ctcactttcg agctcaacca gctgtgctga gagaatctcc ccgtaatcct ttaagctttt
     8161 ggtgtcgagc gatacaccat tgcgctccat gtaagttagc acctcaacta gcggtgcttc
     8221 aagggcaaaa tacagattgg ttaagccctg ctcctcgagc ttagagaaca gtatatgctt
     8281 gagctgcaaa gctaaatcgg catcctcgca ggcatatcgg gtaattagcg tagcctcaac
     8341 attgcgcatg ctaccctgct tggaaccctt cttcccgata agttcctcaa tgctaatagg
     8401 cttgtagctc aggtagattt cggaaaggta atcgagatta tggcgcgagt cgggttctaa
     8461 caggtaatgg gcaagcatgg tatcgtaaag gtgcccctta atgtcaattc catagcttaa
     8521 cagcacctgc atatcgaact taatgttctg tcctaccttg gcaatggttt cggtaccaaa
     8581 tacattggca aactccttaa ctacctcttt tgcctggttt tgatccgatg gtactggaac
     8641 ccaccaagcc ttacgcggcg aaatggcaaa ggataagcca accagctcaa ccgatattgg
     8701 gtcaagccca gttgtctcgg tatcgaaagc aaactcactg gaattttcca gttctttaat
     8761 tagaattcga cgttcctctg tggtttgcgc aatgcggtaa tcaacctcaa aatcgttaat
     8821 ggttttgagg ttaatggctg gctttggtgt ttcggttgca ggcatatcga acagcgagcc
     8881 ctggacgtac tgtggctttt ccacttttgg cgttgcagta aactccttga gcatcgacct
     8941 aaactccagc tcctccagta ttggtactag ctcattggtt ttaggcggtt tcacggcaag
     9001 gtcgttttca atatcaacgt tgagatcgat attggtaaca atacttacca gcttacggtt
     9061 aagcggaata atgttaagtg cagcctcaaa gttctccctt tgtgctgggc taagcttatc
     9121 gagattttga agtagattat cgatgctccc aaactgggaa attaacttag cagcggtttt
     9181 ctcacccacc ttgttaacac ccttgatatt atcggaggca tcgccccaaa gggcaagaat
     9241 atcgataaat tgctttggat cttggatatc gtaatcctta cagaattgct ctgctgtaac
     9301 aatctccatc tcgtttccac tcctacccgg cttggcaatt ataatgttct cgcttaaaag
     9361 ttgaccgtag tccttatcgg gggtcatcat tatcacttca aactccttgg ggtttgcctt
     9421 tgtggcaagt gtgccaataa catcgtcggc ctcaaatccc tccttttcaa taattggaat
     9481 gttaagagcg tttaaaaggt ttttaataac aggaaccgat gccctaatat cctcaggcgt
     9541 ttcatcgcgc tgggctttgt aatcggggta aatgtcgttc ctgaaggttt tgcccgatac
     9601 atcgaatgct accgccacat gggtagggtt aaactttttg atagcatcga gtgtggccct
     9661 gataaaaccg taaatagccg atgtattcat tcccttgctg ttacgcattg ggcgattgat
     9721 aaaggcatag tatgccctgt aaattagggc ataagcatcg agcaatagca ggcgtttttt
     9781 catggttttg gtaaacagtt acaggttgtc ggttgcatac cattcggcat acgatgtggc
     9841 agtctcgtta agttttaggt gatgtagtgt tacattgacc ggaagattag cctttataat
     9901 ttcagcaaag tagtgaatca tattctcaca ggtgggttgg aatggcaggg cctcaatacg
     9961 attgaaaccg gcttccctaa gctttgtatg cgttggtgta ttggccatta cggccacagc
    10021 atggtcaaac ctatcaacaa ttagggggtt aactatgcgt ttaagatcgc caaaatccat
    10081 caccatcccc agctttgggt gcccttgttt atcgataggc tcacctataa cggtaaccgc
    10141 aagtatgtat gaatgaccgt gcatgtgtcg gcataagccg tcgtagccct caagggcatg
    10201 ggccatctca aaccgaaact ctttggttaa acggattttt ggcatatggg taggtttaca
    10261 agttaaaagt taaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntttccc
    10381 aacgtttaca attcgtcgcc aactactctt gtcatcacaa ataccccgca aatatcctcg
    10441 gaatcgtagc tgtgacccac aatctttttt tcatgggtgt aagtaccctc aagcacatcg
    10501 agattgtaat tcagaatggg agtcccatcg gggtgcatca cttccaggcc ttttagaaca
    10561 atagtgtgac cttgaacgtt accctctaca atttgcctta ccatgaatgg ttcttcatcc
    10621 tcaatacact cttcgtactc caggtatcca acaataagat cacctttttg ctccagaaag
    10681 gcaaaaccct tatcggtacc gcactcaaac tcctcagtaa atgtccattt ccctgttaag
    10741 ctatccatgt ataaaaattc aatttttaca aagatagtga acgctaactc aaagtctttg
    10801 tttattgagt aaatttacgc aaattaatga accctttcaa ctttactgtc atgaagttca
    10861 ttggagccca tgtaagcgcg gcaggtgggg ttgagaacgc ccccgtaaat gcccatagta
    10921 taggagccac agcgtttgca ctttttacca agaaccaacg ccaatgggaa gccccgccac
    10981 tcacccagaa aagcattgat gcttttaaag caaactgtga gaaatatggc tacacaccct
    11041 cacaaattct gccacacgat agttacctta ttaaccttgg taaccccgat agggatgcgc
    11101 ttgaaaaatc gcgcaatgca tttctagatg aaatggtgcg ttgccagcag cttggcctcg
    11161 ataggcttaa ctttcatccg ggcagccatt tgaataaaat ttctgaagag gagagccttt
    11221 tgctcattgc cgattcaatt aatatggctt tggagcgcac acagggagta acggcggtaa
    11281 ttgaaaacac gtcggggcag ggaagtaacc ttggctacac ctttgatcaa ataaggtata
    11341 ttatcgacca cgttaacgac caatcgcgtg ttggggtttg cattgacaca tgccatgcct
    11401 ttacggccgg gtacgatata tcgacacccg aagcttttgc caaaaccttt agccatttcg
    11461 atagggtgat tggttttaaa ttccttaagg gcattcacct taacgacacc aaaaaagggc
    11521 taggcagccg tgtagatcgc catgagaacg ttggcgatgg ccttttggga attgagcctt
    11581 tccggctcat tatgaccgac ccacgcttta atggaatccc aattattctt gaaacacccg
    11641 atgagtcgcg ctgggctgag gagattaaga tgctaatgaa gtttgcaaat gctaagtagc
    11701 aaatatcaag ctaaaaaact cgttattccc taataaatta ataacttaca taaaaaaatg
    11761 cgccatggaa atacaagccc tttacccgga atttgaggag gcattggtta acgagcttaa
    11821 gcagttgccg gtaatggaaa ttgatgctgg catggagata cccatgcacg ggaatgttgc
    11881 atcgcatata cctttgggcg ttgaagggtc ggttcgtgtt tttggacgcg acagcgaagg
    11941 acatgaagcc gatttgtatg aggttaaacc gggagtagcc tgtattgttt cgtttacatc
    12001 ggctatgatt gatactccgg ctataaccta tgccaaggca aagcaaaaat caaaaatcat
    12061 tccggttccc aaggagttgt caaacacatg gtttgttaag tacacctcgt ggcgacaata
    12121 cattattggg ctataccaga accgcctcga tgaattaatc aaacagaacg aaagggttgt
    12181 agcccaaaag gaaatgattg aaattcaaaa aaatcagatt atccagagca ttaactatgc
    12241 ccgaaacatt cagaaagcag ttttgcctgt tgagagtcag ctcaaggatt tttttgccga
    12301 ttacttcata ctttacattc ccaaggatat tgtttccggt gatttttact ggacatacta
    12361 ccaaaacgac aaggtatttt ttgctgcagc cgatgctacc ggtcatggag tgccaggagc
    12421 ttttatgagt atgttaggaa tatcgctaat gaatgaaatc tgctataact tcagcggtaa
    12481 cgctgcagac tttttaaaca ccatgcgaaa caaaactaag gaggcactta agcaaaccag
    12541 ctttgactct ggcccccgtg atggattcga tttacaactt tgcattttcc acacccgtca
    12601 aggcattgtt gagtatgccg gcgcaaacaa taaccttttg ctaagcagaa ataaccagat
    12661 ctttgagttt aatgccgata aaatgccagt tggagtagcc tacaaggaag agaaaccgtt
    12721 taccaatcac tccataagtg ttcaggataa tgatatattt tatctatttt ccgatggcta
    12781 taaggatcaa ttcggaggaa ctgaacagca aaagttttcc aagaaaaggt tttacaacct
    12841 gcttagccaa ataagctcaa aaccgcttac tgaacagaaa gaaattcttg aaaagaaatt
    12901 tcacgactgg aagggcagca acacacaggt tgacgatgtt ctggttatgg gcataaaaat
    12961 cagggctaag gaataatgta actgaacttc aaattatgct agagtttcta ttttcaaaga
    13021 aacaaaaata ggccaaggat tgtccctggc ctataagtat gaatttgaaa gatgctttct
    13081 gttaccaacc cagtacgtag gcaaaaatga gtggtgccac aatggtggca tcggactcaa
    13141 tgataaactt tggggtatcg atattgagtt ttccccaggt aattttctca ttgggtactg
    13201 cacccgagta agagccatag ctggtggttg agtcgctaat ttggcagaag tagctccaga
    13261 aaggaactcc ggtacgttca agatcctgat gtagcatggg aaccacacaa attgggaaat
    13321 cgccggcaat acctccgcct atctggaaga aacctactcc ctcagtaccg gcattgttgg
    13381 tgtaccagtc ggctaggaac atcatatact caatacccga ttttacaatt gaaggcttta
    13441 gctcgccggt aatgcagtat gaggcaaaaa tgttacccat ggatgagtcc tcccaacccg
    13501 gaactacgat aggtaaattc ttttcagctg cggctatcat ccaggaattc tttgggtcaa
    13561 tctggtagta ctgctctagc acaccgctct ttagcatttt gtacatatac tcgtgaggga
    13621 agtaacgttc acctttatcc tgtgcctctt tccaaacatc gaataggtgc gactgaagcc
    13681 tgcggaaggc ttcctcatca gggatacagg tatcggtaac gcggttcagg ccgttttgaa
    13741 gcaactccca ctcctgctgt ggggtaagat cgcgatagtg cggtacgcgc ttgtagtggt
    13801 tgtgggcaac caggttcatt aaatcctcct caaggtttgc accggtacac gaaatgatct
    13861 gaaccttatc ctggcgtatc atttcagcta agctgatacc aagctcagcg gtactcatgg
    13921 caccggccag ggtaatcatc atcttcttgc cgctctcaat gtgagctttg tatgcctttg
    13981 ctgcatcaac cagggttgct gagttgaagt gcaggtagtg cttctcaata aacgaggtta
    14041 taggtttact gctgtccatt gctaatagtt ttattgttta atacattgat ggtattctat
    14101 ttcacaggaa ttttctcaac cctacgggta tgacggccac cctcgaactt ggcatcaaga
    14161 aaagccttta ctatttgttt ggcctcgcta acgctaacaa agcgggcagg aatggcacaa
    14221 acgttagcat cgttatgctg gcgggcaagg gtagcaattt ccggtaacca gcacaatgcc
    14281 gaccgtaccc cctggtgctt attggctgta attgataccc cgttaccact accacaaagc
    14341 aatattccta gctggtaatc gcccttttca atggcagttg ccaacggatg agcaaaatca
    14401 gggtaatcaa cgctatcgtc gctgtgggtt ccaaaatccc aaacattata ccccaactca
    14461 gcaagatatt tcttaagctc ctccttagtt gaatatcccg catgatccga cgcaatggca
    14521 agtgtaggtt tactcatatt tgtaacagat tcaagattct ctaaaaatac aacgtttcga
    14581 tttctgaata ctgaattcca cttaaatcat cccagctaat cattaccttt gcaaagataa
    14641 agtgttttta tgttttaagt acaatcattt agctttgctt aagcaatcgg ataccatggc
    14701 aggaatttat atacatgttc cgttttgtag gaaaaaatgc agctactgcg acttttactc
    14761 ggtgggcgaa gctaaaatgg ataaccattt taccgatttt gtgcttaaag agctcagcct
    14821 caggaaccct ttaatagctg atagaatagt aaaaaccatt tactttggag gtggtacacc
    14881 atcgctattg ccagctggca atatcaagag aattatttcc ggtttaggtg atttttttgg
    14941 agttgacccc aatgcagaaa ttaccattga ggtaaacccc gacgatgtta cccttgagct
    15001 ggctgagcaa taccaagcaa ttggcgttaa cagggtaagc attggcattc aatcgtttaa
    15061 caacgatgag ttgatttttc ttggccgacg acacgatgtt ggtaaagcaa ttgaggctat
    15121 tcgtatattt aggaaagcta gtattgcaaa catcagcatc gatttaattt atgggctacc
    15181 ccattcaacg ttgaaaagtt gggaacactc gttaaatgtg gcatttgggt tagatattca
    15241 gcacctatcg tgctaccacc ttacctacga ggagggaacc ccgcttaccc gaagggtagc
    15301 caagggaact gtaattcccc ttgatgaaga gctgagcaag gcccagttcg atttgctatg
    15361 tgagatgtcc aataattatg acttcattca ctacgaaata tcgaacctgg caaaaccggg
    15421 ctatatctca aaacacaaca gcggttactg gttgggcgaa gaataccttg gcttaggccc
    15481 ttcggcacac tcatttaaca ggtcaatgcg atggtggaat ccatcgtcca ttaccggttg
    15541 ggaaaaggaa gttgactcag ccaattttcc caaaaacatg gaagcaatcg gtgaggttac
    15601 ccgctttaat gagctactca ttacccgcct acggactatg tggggtgtaa accttgaaga
    15661 ggttgcgaat ggattctcac ctgaaatata taaccgtctt cgcaaaaaaa tcataccctt
    15721 tactgcgtcg ggtaaaatgg taatggaaaa caacatcctt aaaattcc
//
LOCUS       DAOSAL010000062        15476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_2615_length_15476_cov_49.377148, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000062 DAOSAL010000000
VERSION     DAOSAL010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 15476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15476
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2615_length_15476_cov_49.377148"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="PK049_08755"
     CDS             complement(<1..401)
                     /locus_tag="PK049_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOK61689.1"
                     /translation="MGKEKKFITCDGNYAAAHIAYMFSEVAAIYPITPSSTMAEYVDE
                     WSAFGRKNIFGDTVKVVEMQSEAGAAGAVHGSLQSGALTSTFTASQGLLLMIPNMYKI
                     SGELLPGVFHVSARSIAAQALSIFGDHSDVM"
     gene            717..1937
                     /locus_tag="PK049_08760"
     CDS             717..1937
                     /locus_tag="PK049_08760"
                     /inference="COORDINATES: protein motif:HMM:NF017072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOK61690.1"
                     /translation="MPKHYKIGILRETKTPPDRRVALTPEQVVEVIKKFDNVDVVVQP
                     SELRCYKDDEYLRLGVKLQDDLSDCDLLVGVKETKIPTIIPGKTYMEFAHVAKKQPHN
                     QKLIQAFAANGNTVIDYEYLTDKNGVRLVAFGHWAGVVGSYNALRAIYIKHKLGELPP
                     AHTLHDYNELKGILKKIKLPPLKFVITGGGRVAGGAIEVLGQAGIAEATHEEFLTKEF
                     DHPVYTRLDPWHYAKRNDGKPFEWDYWVNNPADHQSTFLPYAQTADVFIACHYWDFRS
                     PHFFTVDDMKQPDFRISIIADVSCDIPGPIPSTIKASTIADPFFDFNPATGKEEPAFS
                     CGMNVTVMSIDNLPGELPRDASEFFGRLLIDKVLPHLLCDDCEGVIERATILKDGKLT
                     PRYAYLQDYLEGKE"
     gene            complement(1967..2953)
                     /locus_tag="PK049_08765"
     CDS             complement(1967..2953)
                     /locus_tag="PK049_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOK61691.1"
                     /translation="MNTFGRIVRVSIFGESHGAMVGVTIDGLPHGIKLNADDFTDDLA
                     RRRAGAIGTTPRVEGDIPEIATGWFNGYTTGAPLTILFRNENTKSGDYDKLKDVPRPG
                     HSDFVAYHKFDGYNDYRGGGHFSGRLTLGLVAAGVVAKKVIPQVSIEARLIEAGGKTN
                     IDEAIAEAQKQNDSIGGIVECVATGIPIGLGEPFFDSAESVISHLAFSIPAVKGIEFG
                     SGFAASRMKGSEHNDPIIDSKGTTATNNAGGVNGGITNGNPLVFRIAVKPTSSIAKPQ
                     QTLNIKSEKIETLMVEGRHDTCIALRVPVVVEAITAIALADMLLIERTRKNR"
     gene            complement(2963..4210)
                     /gene="aroA"
                     /locus_tag="PK049_08770"
     CDS             complement(2963..4210)
                     /gene="aroA"
                     /locus_tag="PK049_08770"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOK61692.1"
                     /translation="MKKLVKPSAVKGTVQAPASKSVAQRAIALATLAEGQSIIYNAGN
                     SNDCLAAIDTCKKLGAHIVGDGSKLTITGGPKSPNDTLHCGESGLSIRMFAAIASIFN
                     TPVTLTGSGSLTNRPMNMVTDGLAQLGVRCTTNNGKLPITVQGPIQGGYITVDGSQSS
                     QALTGLLMASPFAQNDVTIKVDNLNSKPYIDLTIETMKAFGVRVDELPGNTYRIAVNQ
                     KYKASNFNVEGDWSGAAFMLVAGAIAGEVEVININPKSLQADRAIVEALLSAGASLSI
                     RENSIVAKRANLKAFDFDARNCPDLFPPLVALAANCNGQSKILGVNRLHGKESDRALT
                     LQQEFGKMGVKIELVGNLMVVNGTSIKSAITYSHGDHRIAMACAVAALSANGDVEIEN
                     AEAVNKSYPNFFDDLYKMQVDNF"
     gene            complement(4179..5099)
                     /locus_tag="PK049_08775"
     CDS             complement(4179..5099)
                     /locus_tag="PK049_08775"
                     /inference="COORDINATES: protein motif:HMM:NF020090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOK61693.1"
                     /translation="METYAVFGNPILHSKSPQLFNSVFRGMGIDAKYTRIRPRSAHDL
                     TNIILNYSIKGANITTPFKEDVIKYLNHVSDEVAEIHGVNTITNQNGKLTGYNTDWNG
                     VVNALVNAGVTLIGRKVLVLGAGPAATAAVYGLKKAGAVVHIANRTTQKAIEIGKRLN
                     VNYIDFDEVSHKLSYFEVVVSALLPNANPFLGVNIPRNLVLLDANYRPSSLSEQFMSF
                     GCRVISGKQWLIHQAVEAFKIFTGKAPDVEIMSVAVENELTLNYLKAQWIQRVDNKKL
                     FTDYDLLISASNDNEFKQFLDEEISKTFGS"
     gene            complement(5101..5754)
                     /locus_tag="PK049_08780"
     CDS             complement(5101..5754)
                     /locus_tag="PK049_08780"
                     /inference="COORDINATES: protein motif:HMM:NF013640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HOK61694.1"
                     /translation="MKAIRPDICVSIGNVDFHAISNLLPKLSLAEIRIDLLNLNRDEL
                     KLLFGLHDNLIATYRPKESEFDVMLNLLTDTIGYGAAWVDVDIDTPTEIIREITAIAQ
                     AANCKVIISYHNYTETPTYARLKKITENAQTYDVSLVKIACMANSTSDCARVLSLYEE
                     NTRIVAFCMGQVGTITRVAAPLLGAPFTYAALPGNQTAPGQLDYIYLESLLDSLKPL"
     gene            complement(5767..6786)
                     /gene="aroB"
                     /locus_tag="PK049_08785"
     CDS             complement(5767..6786)
                     /gene="aroB"
                     /locus_tag="PK049_08785"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOK61695.1"
                     /translation="METILIEGQNGKSTIIVDERFEEVTKYLPKKKHFIITDKNIEKY
                     YATVFPEAPFFSINPGEPSKQLGYIESIYRWLLENNADRNSFILGIGGGVVCDIAGFV
                     ASTYMRGVEFGFVSTSLLSQVDASVGGKNGVDLDGYKNIIGTFTQPRFVLCDTRLLKT
                     LPHEELVNGFAEMVKHALISDIKLFEQLEHDCIALLNLNQPEISKHIAESIRVKASIV
                     TADERESGIRKLLNFGHTWGHAVETLTGISHGKAVSIGMEFACRLSMEKGLISNRDYL
                     RIINLLQSFGLPTCMNIEPSRVFKTLVKDKKRNANSIDFVLLMGIGVPKIETMALEEL
                     KKFAE"
     gene            complement(6813..7916)
                     /locus_tag="PK049_08790"
     CDS             complement(6813..7916)
                     /locus_tag="PK049_08790"
                     /inference="COORDINATES: protein motif:HMM:NF012991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase type II"
                     /protein_id="HOK61696.1"
                     /translation="MANEKLKTEEGTNPSIQKDAKPLIIAGPCSAESPQQLVEVAQEL
                     KKNGFVNYMRAGVWKPRTTPNSFEGYGTEALIWLNQVKQETGMPFATEVATERHVYEC
                     LKFGADLLWIGARTVSNPFAIQEIASALRGVQIPVLVKNPLSPDLKLWEGAINRLRLA
                     GVKEVGAIHRGFTVWHASPFRNFPMWEVALQLKANNPELRVISDPSHISGDAQYIELL
                     SNRAINLGFDGLMVEVHPNPKIALSDAKQQLTPNQFNLLISNIFEAKQANTELMNELR
                     AEIDALDELLVWALSNRMAIATEIARVKSETGQKIIQPDRWNEVLNRVIGKGHKMGLR
                     TDFVKRLFNSIHRESLQTQEAITQNRTEMVKFG"
     gene            complement(7891..8793)
                     /locus_tag="PK049_08795"
     CDS             complement(7891..8793)
                     /locus_tag="PK049_08795"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOK61697.1"
                     /translation="MELKNLTIAVIGLGQIGASLAAGLKGKVNTIVGFDIDPLNEEFC
                     MNAGIIDTSNTLEEICQMADVVAIATPVDQVMPIASRILSASGKPLAVFDVGSTKSII
                     NIVLSTLPERSKFVSSHPMAGSAEQGAVNASASLFRNKLVYICDEHLCSKQAVELITG
                     LWDLLGARVEFIDSNQHDKLMANVSHLPQLVSYALAIASVNRIGSLNQTMEAASSGFD
                     SMTRLAKSSASMWLPIVKQNKENILSAIVEFQKQLNRIELAIRQNDDQTLQWLIVQAN
                     GIRKAFENQQNQNILNHGKRKVKN"
     gene            complement(8801..9634)
                     /locus_tag="PK049_08800"
     CDS             complement(8801..9634)
                     /locus_tag="PK049_08800"
                     /inference="COORDINATES: protein motif:HMM:NF012998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HOK61698.1"
                     /translation="MQPIKVAIQGFKGAFHQDAAELYFNTPLELVECLTFKELVNSVT
                     EGRAEKGVMAIENSLVGSILQNYTLIRESDLTIEGEVFLRISQHLIGISGQRVEDIKE
                     IHSHPMAIAQCDDFIQSIPHVKVFATHDTALSARDIALNQIKGVAAIASERAAREYGL
                     EVIASSIESNLNNYTRFVVLGKTSKHSYSSANKASVAFSLAHKVGSLQAALQSFSRYG
                     VNLTMLQSLPQGGNPWEYYFHTDAVFRHIDDFELALDSLKTIANEVKVLGIYTTGIKT
                     N"
     gene            10058..10633
                     /locus_tag="PK049_08805"
     CDS             10058..10633
                     /locus_tag="PK049_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOK61699.1"
                     /translation="MIKILLLDNYDSFTYNLYHYIDLTGGFIVDVIRNDALKLASINS
                     YDGIVLSPGPGLPKESGLLMNVIDNYHSKKKIFGVCLGHQALAEYFGAKLLNLPEVLH
                     GIQTPIKILEPKHYIFRDIPSTIEVGRYHSWVVNPIDLPDCLTVTAIDYDNQIMALCH
                     KKYDICSVQFHPESIMTPYGMKIILNWLDGF"
     gene            complement(10630..11280)
                     /gene="rpe"
                     /locus_tag="PK049_08810"
     CDS             complement(10630..11280)
                     /gene="rpe"
                     /locus_tag="PK049_08810"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018462549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOK61700.1"
                     /translation="MTRLVAPSMLSADFANLSCDIEMVNKSQADWFHLDIMDGVFVPN
                     ISFGFPVVERIKSLATKPLDVHLMIVDPDRYLERFREAGADWLTVHYEACTHLNRTLS
                     RIRELGMKAGVSVNPHTPVELLTDILEYADLVLIMSVNPGFGGQKFIPNSIGRIERLR
                     KLIDQKGLSTLIEVDGGVGPSNARELYQAGANVLVAGNAVFKADNPLAAIEMIKNA"
     gene            complement(11351..12214)
                     /locus_tag="PK049_08815"
     CDS             complement(11351..12214)
                     /locus_tag="PK049_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HOK61701.1"
                     /translation="MRQLKIIKQVTNREAVSLDKYLHEIGKVDLLTAEQEVTLARKIK
                     EGDQEALAKLVKANLRFVVSVAKQYQNQGLSLPDLINEGNVGLIKAAQRFDETRGFKF
                     ISYAVWWIRQSILQALAEQARIVKLPLNKIGSINKVNRALAELEQRFEREPTIDELSE
                     MLNLAPDDVKEALKGNNRHLSMDAPITQDEEGSMYDVLLSPDSPMPDKGLLNESLRKE
                     IERVLSTLSPREANIIRLYFGLNGKHPLTLEEIGEEFDLTRERVRQIKEKALKRLKQT
                     TRSKILKSYLG"
     gene            12413..13402
                     /locus_tag="PK049_08820"
     CDS             12413..13402
                     /locus_tag="PK049_08820"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOK61702.1"
                     /translation="MHFSVIIPVYNRPGEVEELLQSLAGQGYNSFEVIIVEDGSTVPC
                     SDIVNKFASELKINYYFKENTGPGLTRNYGAERAKGEYLIFFDSDCIIPNNYFTRVEE
                     ELAAEYVDFFGGPDATHPSFTNVQKAIGYSMTSFFTTGGIRGGRVKLERFTPRSFNMG
                     INAEVFHSVGGFSNMRFGEDVDLSLRLFEKGFTSRLFPKAFVYHKRRTDFRKFFKQVF
                     NSGMARINLSKNHKGSLKMVHLLPSGFVLVMSAAILLSWAFPIILTLPLLYTFAIMFD
                     SLLKGNSLTVAILSIVAAWVQLYGYGLGFIYATWLWVVHGKRPNGAFTKNFYR"
     gene            complement(13403..14071)
                     /locus_tag="PK049_08825"
     CDS             complement(13403..14071)
                     /locus_tag="PK049_08825"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOK61703.1"
                     /translation="MNKVIALSPIFRGLTENEVAHLLNETPHTIRSFSKGQTIAQRTE
                     EVRNLCILVEGSVKGEMVDFSGKILKIEEMHAPMPIAHAFLFGENNRYPVDVIALENC
                     KLLYIPQVEFLGLMQKNAKVLTNYLNSISNRAQFLSNKLWFLSFKTIKEKIAHYLLNL
                     SKSEIRTTLILPKSHQELAEFFGVTRPALARVFSEMQDEGIIHINRREITILNRNKLL
                     EMIK"
     gene            14128..14721
                     /locus_tag="PK049_08830"
     CDS             14128..14721
                     /locus_tag="PK049_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61704.1"
                     /translation="MPVKYQNGQVVCVQEHKPIAFNPWKHHCGFVCNQIQLIRGEGGG
                     KLNELLGYIGGTLLDFYIGELPVYTIVNEVKKYFMDLGVANVDDFKHWLGLPETTFKS
                     IYLSDGQCWVIRLGQNDEYYIHIHPGRHSKLTIRCRPTTLKTAIAFRYLFGFEDESLE
                     IDKLNYARKLAGLPPVESLNSASALQNFISNLRVNNR"
     gene            15031..>15476
                     /locus_tag="PK049_08835"
     CDS             15031..>15476
                     /locus_tag="PK049_08835"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOK61705.1"
                     /translation="MNLSLSKAFSFISYLLIISCSTETERLTTNVFRYNESKGITSLD
                     PAYARNLALIWPVSQIFNGLVQFSDSLTVEPSIAKRWSISPDGLTYTFLLRNDVYFHD
                     SPVFKSGKGRRVTAQDFVYSFGRILNPTTASPGMWVLSVLDKENPN"
BASE COUNT         4115 a         3299 c         3235 g         4827 t
ORIGIN      
        1 ctcatcacgt cgctgtgatc gccaaagatc gatagcgctt gcgctgcaat actacgagcc
       61 gaaacgtgga atacacccgg gagtaactct cccgaaatct tatacatgtt ggggatcatc
      121 agcaacaacc cttgcgatgc agtgaaggtt gatgttaatg cacctgattg caatgagccg
      181 tgcactgctc ctgcagctcc ggcctcgctt tgcatttcaa ccacctttac agtgtcgcca
      241 aatatgttct ttcgaccaaa tgccgaccac tcgtcaacat actcggccat ggtggacgat
      301 ggggtgatgg ggtaaatagc tgcaacctca ctaaacatat atgctatgtg cgcagcagca
      361 tagttcccat cacaagtaat aaattttttt tcttttccca tttcagtttg attgttatga
      421 tggttagtaa taatttttaa gcccatgcaa agttaatact attacctgat ttttttcatg
      481 ctttacaaca atttacagcg atgtgtttac acgctaacaa cctgaaaacg tttgaaataa
      541 acatctgtta ttggagtata atattacaat tcgatatatt ttttgctcga tatgttataa
      601 atcgtaagca ataattcttt tatttaacat ccatttaaaa attttttcaa atgtgatatc
      661 gttttattag tttcgcaaaa ttcaattttt gctaaaaaaa gttaaaacca agccatatgc
      721 ctaaacatta taagattgga attttaaggg aaaccaaaac gccacccgat aggcgcgtag
      781 ccttaacccc cgagcaggtt gttgaggtga ttaagaaatt cgacaatgtt gatgttgtgg
      841 ttcaacccag cgagttaagg tgctataagg atgatgaata cttgcgcctt ggggtaaagc
      901 tccaggacga tttatcggat tgtgacttgc tcgttggcgt taaggaaacc aaaataccaa
      961 caatcattcc gggtaaaact tacatggaat ttgcacatgt ggctaaaaag cagcctcaca
     1021 atcagaaact gatacaggct tttgcggcta atggcaatac ggttattgac tatgagtatc
     1081 ttaccgataa gaatggcgtt cgtttagttg catttggtca ctgggcagga gttgttggat
     1141 cgtataatgc cctaagggca atttacatta aacacaagtt aggagaattg cctcctgctc
     1201 atacgttgca cgactataat gagctaaagg gcattctcaa aaaaatcaag ttacccccgt
     1261 tgaagtttgt aattaccggt ggtggtaggg tggcaggtgg tgccatagaa gtgcttggac
     1321 aagctggtat agctgaagct acacacgaag agttcctgac caaggaattc gatcatccgg
     1381 tttacacccg cctcgaccca tggcattacg ccaaacgcaa tgacggtaaa ccctttgaat
     1441 gggattactg ggtgaataat ccagccgatc accagagtac tttcttaccc tatgcacaaa
     1501 ctgccgatgt gtttattgct tgccactact gggatttccg ttcaccccac ttcttcaccg
     1561 ttgatgatat gaaacaaccc gatttcagaa tctcaatcat tgccgatgtg agctgcgata
     1621 tacccggtcc aataccctca accataaagg cctccaccat tgccgatcct ttcttcgact
     1681 ttaatccagc taccggaaag gaagagcccg cattctcatg cgggatgaac gttaccgtga
     1741 tgtcaatcga taacttaccg ggcgaattac cccgcgatgc cagtgagttt tttggccgtt
     1801 tgctcatcga caaggtgcta ccgcacctgc tatgcgacga ttgcgaaggt gtgatagagc
     1861 gagccactat cctaaaagat gggaagttaa caccccggta tgcttacctt caggattacc
     1921 tcgaagggaa agagtgaatg gtaggaattt gaaaccggtg gcataatcac cggttttttc
     1981 ttgtcctttc aattaatagc atatcggcta aagcaatggc tgtaatggcc tctactacaa
     2041 ccggaaccct tagcgcaatg caggtatcgt gtctgccttc aaccattaga gtttcaattt
     2101 tttcggattt tatattcagg gtttgctgcg gtttagcaat gctcgatgtt ggtttaaccg
     2161 caattcggaa cacaagtggg tttccgtttg tgataccacc gttcacccct ccggcattgt
     2221 tagtagctgt agttccttta ctatcaatta tcgggtcatt atgttccgag cctttcatgc
     2281 gcgacgcggc aaagcctgat ccaaactcaa taccttttac ggcaggaatg gaaaacgcca
     2341 gatggcttat taccgattca gctgaatcga aaaatggttc accgaggcct atggggattc
     2401 ccgtggcaac gcattccaca attccaccta tggaatcgtt ttgcttttgg gcttcggcaa
     2461 tggcttcgtc aatattagtt ttgcctcctg cctcaatgag ccttgcttca atgctaacct
     2521 ggggtattac ctttttggcc accacaccgg ctgcaaccag cccaagggtt agtcggcccg
     2581 agaaatgtcc gcccccgcgg taatcgttgt aaccgtcaaa cttatggtat gcaacaaaat
     2641 cggaatgccc gggacgggga acatctttca acttatcgta atcgcccgat tttgtattct
     2701 cattcctgaa gagtattgtt aaaggggcac ctgtagtgta gccgttaaac caaccagtag
     2761 caatctcggg tatatcgccc tcaacacggg gagtggttcc tattgcacca gccctacggc
     2821 gggctagatc gtctgtaaag tcgtcagcat ttagctttat tccatgtggc aagccatcaa
     2881 tagttacacc taccatggct ccatgcgatt cgccaaagat actgaccctg actattctac
     2941 caaatgtgtt catatggttg ccctaaaaat tatcgacttg cattttgtaa agatcgtcaa
     3001 aaaagtttgg atacgatttg tttaccgcct cagcattctc aatttcaaca tctccgtttg
     3061 ctgaaagtgc tgcaacagca catgccatgg caatgcgatg gtcgccatgc gagtatgtaa
     3121 ttgccgattt gatactcgtt ccatttacta ccatcaggtt gccaacaagt tcaattttaa
     3181 cgcccatttt cccaaactct tgctgtaggg taagcgctct gtccgattcc tttccgtgta
     3241 aacggtttac cccaagtatt tttgactgtc cattgcagtt agctgcaagc gccacaagtg
     3301 gcggaaaaag gtcaggacag ttacgtgcat caaaatcgaa agccttcagg ttggctcttt
     3361 tggctactat gctgttttca cgaatcgata gcgaagctcc ggcactcagt aaggcttcaa
     3421 ctatagccct atcggcttgt aacgattttg ggttaatgtt tataacctca acctcacctg
     3481 caattgcccc agccaccagc atgaatgcgg ctccactcca gtccccctcc acgttaaaat
     3541 tactggcttt atatttctgg tttacggcta tgcggtaagt gttgcctggc agttcatcaa
     3601 ccctaacgcc aaatgccttc atggtttcta tggtaaggtc gatatatggt ttgctattca
     3661 agttatcaac cttaatggtt acatcgtttt gggcaaaagg cgaagccatg agtaagccgg
     3721 taagtgcctg tgaactttgt gagccatcta cagtaatgta tccaccctgt atgggtccct
     3781 gaacagtaat tggtaacttc ccgttgttag tggtacacct aacgccaagc tgcgctaaac
     3841 catcggtaac catgttcatt gggcggttgg ttaatgagcc gctaccggta agggttacgg
     3901 gcgtattgaa tatggatgca atggcagcaa acatcctgat gcttaacccc gattcgccac
     3961 aatgcaatgt atcattaggg cttttggggc ctcccgtaat ggttagtttg ctcccatcgc
     4021 ccactatatg ggcaccaagt ttcttacaag tgtcgatggc ggcaaggcaa tcgttactgt
     4081 tgcctgcatt gtaaattatc gattgtcctt cggccaaggt tgccaatgca attgcccgtt
     4141 gggcaacgct tttcgatgct ggcgcttgaa ccgtcccttt aactgccgaa ggttttacta
     4201 atttcttcat ccaagaattg cttaaattca ttatcgttgc ttgccgatat aagtaaatcg
     4261 taatcagtaa acaatttttt attatctacc ctttgtatcc attgggcttt caggtagtta
     4321 agtgttaact cattttctac cgctacgctc atgatttcaa catcaggcgc tttaccagta
     4381 aaaattttaa acgcctccac tgcttgatgg ataagccatt gcttacctga aattacccta
     4441 cacccaaagc tcataaattg ctcgcttagg ctcgaagggc ggtaattggc atcgagtaga
     4501 actagatttc ggggtatgtt aacccccaaa aaagggtttg cgttagggag taaagccgaa
     4561 actactacct caaagtagga gagtttatgg gaaacttcgt caaaatctat gtaatttacg
     4621 tttagtcttt tacctatttc aatggctttt tgggtggttc tgttggcaat atgcaccact
     4681 gcacctgctt ttttcagtcc atatacagca gctgttgcag ctggaccagc acctaatacc
     4741 agcactttcc tacctattag ggtaacacct gcgttaacca aagcatttac aacaccgttc
     4801 caatcggtat tgtaaccggt aagtttgccg ttctggttgg ttatggtatt taccccgtgt
     4861 atttcggcta cttcatcgct aacatggttc aggtatttga taacatcttc cttaaagggt
     4921 gtggtaatat tagcaccttt aatactatag tttaggatga tgttggttag gtcatgtgca
     4981 cttctggggc gaatcctggt gtatttggca tcgataccca ttcccctgaa aacgctattg
     5041 aatagctgtg ggcttttgct gtgcagtata ggattaccaa aaactgcgta ggtttccata
     5101 ctataggggt tttagggagt caagtaggga ttctaagtat atatagtcaa gctgacccgg
     5161 tgcagtttga tttccgggta atgctgcata ggtgaacggg gcgcctagta atggagccgc
     5221 aacgcgagta attgttccaa cctgccccat acaaaaagca actattctgg tattttcttc
     5281 ataaagggaa aggacgcggg cgcaatcgct agtactgtta gccatacagg ctattttaac
     5341 taaagataca tcataggttt gggcattctc agtaattttt tttagcctag catatgtagg
     5401 ggtttcagta tagttatggt atgagattat taccttgcag ttagcggctt gggctatagc
     5461 tgttatttcc ctgataattt cagtaggcgt gtcaatatca acatcaaccc aggctgcgcc
     5521 atacccaata gtatcagtaa ggagattaag cattacatcg aattctgact ctttaggccg
     5581 gtaggttgca ataaggttat cgtgtaagcc aaatagcagc tttagctcat ccctgttcaa
     5641 attgagcaaa tcaatcctga tttcggcaag cgaaagtttg ggtagcaaat tgctaattgc
     5701 atggaaatca acatttccta tactaacaca tatatcgggg cggatagctt tcatgcatca
     5761 taacctttat tctgcaaact tttttagttc ctctaatgcc attgtttcaa ttttagggac
     5821 tccaattccc attaaaagaa caaagtcgat actgttggca ttccgctttt tgtcctttac
     5881 cagagttttg aataccctac tgggttcaat gttcatgcag gttgggagtc caaatgattg
     5941 tagcaggtta ataattctca ggtagtccct gttgctaata agtcctttct ccatggagag
     6001 cctgcaggca aactccattc caatgctaac cgccttaccg tgcgatattc cggttagtgt
     6061 ttcaacggcg tgaccccatg tatggccaaa attgagcagc tttctaattc cgctttcacg
     6121 ttcatcggca gtaacaatgc ttgctttaac tctaatggat tccgctatat gttttgatat
     6181 ttcaggttga tttaggttaa gcagagcaat acagtcgtgc tcaagctgtt caaaaagttt
     6241 gatatccgag attagggcat gctttaccat ctcggcgaaa ccattcacaa gttcctcgtg
     6301 gggcaatgtt tttagcaagc gagtatcgca aagcacaaat cggggctggg taaacgttcc
     6361 aataatattc ttatatccat caaggtcaac cccatttttg ccgcctacac ttgcatcaac
     6421 ctgtgaaagt aagctggttg acacaaaccc aaactcaacc ccacgcatgt aggtggaagc
     6481 cacaaaacca gcaatatcgc aaactacacc tcctccaatt cccagtataa agctattacg
     6541 atcggcgttg ttctccaaca accagcggta aatactctca atatacccta actgttttga
     6601 tggttcaccc ggattaattg agaaaaaggg tgcctctgga aacaccgtgg cataatactt
     6661 ttcaatgttt ttgtcggtaa taatgaaatg ctttttttta ggaagatatt tggtaacttc
     6721 ctcaaatctt tcgtcaacaa ttattgttga tttaccgttt tggccttcaa ttaaaatggt
     6781 ttccattctt gcatttaagg attatgccaa agttaaccaa attttaccat ttcagtcctg
     6841 ttttgggtga ttgcttcctg tgtttgtaac gattcgcgat ggatgctatt aaaaagtctt
     6901 ttcacaaaat cagttctgag ccccatttta tgtcccttcc caatcaccct gtttaatacc
     6961 tcgttccagc ggtcgggttg aatgattttt tgacccgttt cacttttaac gcgggctatt
     7021 tcagttgcta tcgccatccg gtttgacaat gcccaaacaa gtaattcatc caaggcatca
     7081 atttcagctc ttagctcatt cattagctct gtattagcct gttttgcctc aaaaatattg
     7141 ctaatcaaca agttaaattg attgggtgtt agctgttgct tggcatcgct aagggctatc
     7201 ttagggtttg gatgtacttc aaccattagc ccgtcgaatc caaggttgat tgcgcggttt
     7261 gagagtagct caatatactg cgcatcgcca gagatatggc tagggtctga aataaccctg
     7321 agctcaggat tgttagcttt tagctgtagg gctacctccc acattgggaa gtttcggaaa
     7381 ggcgatgcgt gccaaacggt aaacccccgg tgtatggcgc caacctcttt tactcctgcc
     7441 agccttagtc ggttaattgc accctcccag agctttaggt cggggcttaa cggatttttt
     7501 accagaaccg ggatttgaac acccctcaaa gccgatgcaa tttcctgtat agcaaaggga
     7561 ttggaaacgg tgcgtgcacc aatccatagc agatcggcac cgaacttcag gcactcgtaa
     7621 acatgcctct cggttgccac ctcggtggca aagggcatac cggtttcctg ctttacctgg
     7681 ttaagccaga ttagggcttc agttccatag ccctcaaatg agttaggggt tgtgcgcggc
     7741 ttccatactc cagcccgcat gtagttaaca aacccattct ttttaagttc ctgagccact
     7801 tctaccagtt gttgtggcga ctcagcactg caaggccctg caataatcag tggcttggca
     7861 tctttttgga ttgatgggtt agtgccttct tcagttttta acttttcgtt tgccatggtt
     7921 tagtatgttt tgattttgtt gattttcaaa tgcttttcgt atgccatttg cttgaactat
     7981 aagccattgt aacgtttggt cgtcgttttg cctaattgcc aattcaatcc gattgagttg
     8041 tttctggaac tcgacaattg cgctgaggat gttctccttg ttttgcttaa ctatgggtag
     8101 ccacatgcta gcgctacttt ttgccaagcg ggtcatggaa tcaaatccgc tggatgcagc
     8161 ctccattgtt tgatttaaag agccaattct atttactgaa gcaattgcca aggcatacga
     8221 caccagttgt ggtaggtgcg atacgtttgc cattagcttg tcgtgttggt tggagtcaat
     8281 aaattcaact cttgcaccca gtaaatccca tagccctgta attaattcta ccgcctgctt
     8341 gctacaaagg tgttcatcgc atatgtaaac tagtttattc ctaaataggc ttgcgcttgc
     8401 atttactgca ccttgttctg cacttccagc cataggatgc gacgaaacaa atttactccg
     8461 ttctggaagg gttgagagta caatatttat tattgatttt gtagatccaa catcgaacac
     8521 agctaatggc ttaccacttg cactaagtat tcttgatgca attggcatta cttggtcaac
     8581 tggtgttgct attgcaacaa catctgccat ttggcaaatc tcctccagtg tattggaagt
     8641 gtcaataata ccagcgttca tgcaaaactc ctcgttaagg gggtcaatat caaaaccaac
     8701 gatggtatta accttaccct ttaatcctgc tgccagtgaa gccccaattt ggcctaagcc
     8761 aataactgca atggttaaat tctttagttc cataccatca ttaatttgtt ttaatgccag
     8821 ttgtatatat tccaagaacc ttaacctcgt tagcaatggt ttttaatgag tctagggcta
     8881 actcaaaatc gtcgatatga cggaaaacag catcggtatg gaagtagtat tcccatgggt
     8941 tgccaccttg tggcaacgac tgcaacatgg ttaggtttac accatagcgg gaaaatgatt
     9001 gtagtgctgc ctgtaggctg ccaaccttgt gtgcaagtga aaatgcgaca cttgctttat
     9061 ttgcagagct ataggaatgt tttgaagttt tacctaagac aacaaagcgg gtatagttgt
     9121 taaggttcga ttcaatggaa ctggcaatca cctccaatcc gtattccctt gccgctcttt
     9181 cgcttgctat ggcagcaaca ccttttattt ggttcaaagc aatatccctt gcacttaggg
     9241 cagtgtcgtg ggttgcaaat acttttacat ggggtatgct ctgaatgaaa tcatcgcatt
     9301 gcgcaattgc catgggatgt gagtgaatct cctttatatc ctcaaccctt tgaccgctta
     9361 tacctataag gtgctgcgat atcctcagga aaacttcccc ctcaatggtt aaatccgatt
     9421 ctcgaattag tgtgtagttc tgcaaaatgc ttcctactaa tgagttttca attgccatta
     9481 cacctttttc ggctcgtcct tcggtaaccg agttaaccaa ctctttgaag gtaaggcatt
     9541 caaccagttc gagaggtgta ttgaagtaaa gttccgctgc atcctgatgg aaagcgccct
     9601 tgaaaccctg tattgctact tttattggtt gcatttgatt tgcattaagt tttattaaat
     9661 taaaaagccc tgtttctggc agggctcaat tagtatcatt aaataaatgg agtagttgag
     9721 cccgctggtt aggggaagta gaaaaagtaa tagtaaaaca taaccttaca tgaggttacc
     9781 cttgtagtgg gttgtatgta gatgcgttta ctcataaaaa aagccccgct attgcggggc
     9841 tggtttattg ttaactggtt ataccttttt ataatataca acaattaccc agcccacaat
     9901 tactaaagta gaagtagtag ctgtaaaagt agttgttgtt tattgctctc attcttttct
     9961 tccttgtttt gcaatattaa gtaaagtaat taacaattca aaagaatgaa taagattatt
    10021 tttgtaagaa tttcattttt aatagttggc tatcggtatg attaaaatat tgcttctgga
    10081 taattacgat tcgtttactt acaatcttta ccattatatc gatttaaccg gtggctttat
    10141 cgttgatgtt attcgtaacg atgctctaaa acttgccagc attaatagtt acgacggaat
    10201 tgtgctttcg cccggacccg gattacccaa agaatcgggt ttactaatga atgtaattga
    10261 taactaccac tcaaaaaaaa agattttcgg cgtttgttta gggcatcagg ctcttgctga
    10321 atattttggg gctaaactgc tcaatttgcc tgaagttttg cacggaatac aaaccccgat
    10381 taaaatactt gagccaaagc attatatttt tcgtgatata ccaagtacta ttgaagtggg
    10441 tcggtaccat tcatgggtag ttaatcccat tgatctacct gattgtttaa cggttactgc
    10501 cattgattac gacaatcaga ttatggcgtt atgtcataaa aaatatgata tctgctcagt
    10561 ccaatttcac ccagaatcta ttatgactcc ttacggaatg aaaattatct tgaactggct
    10621 tgacggtttt taggcgttct ttatcatctc aattgctgcc aacggattat cggccttaaa
    10681 aactgcgttt ccggctacaa gcacgtttgc ccctgcttgg taaagttcac gggcgttgct
    10741 tgggcctacg ccaccatcaa cctctatcag ggttgatagc cctttttggt cgataagctt
    10801 tcttaatcgt tcaattctac caatgctgtt gggtatgaat ttttgtccgc caaacccggg
    10861 gtttactgac atgattaaaa ccaaatcggc gtattcaaga atatcggtta gcagttcaac
    10921 gggtgtgtgt ggattaactg aaacacctgc cttcattcca agttccctga tacgtgaaag
    10981 agtcctatta aggtgtgtgc aagcttcgta gtgaacggtt agccagtcgg cgccagcctc
    11041 gcggaaacgc tcaaggtaac ggtcggggtc aactatcatt agatgaacgt ccaatggttt
    11101 agtagccaga gattttattc gctcaactac tgggaaacca aacgatatgt tgggaacaaa
    11161 tactccgtcc ataatatcca ggtgaaacca gtcggcttgg ctcttgttta ccatttcaat
    11221 gtcgcatgaa aggttggcaa aatcggccga aagcatcgat ggggctacta aacgtgtcat
    11281 cattatcttg ttttttgcaa aagtagctaa aaataaaaaa aaacgacccg atgggccgtt
    11341 aagtaattgg ctatcctaag tagcttttga gtattttgct tcgtgtagtt tgctttaagc
    11401 gcttgagcgc tttttcttta atttgccgaa ctcgttcgcg agttaggtca aactcttcgc
    11461 ctatctcttc aagggtgaga ggatgcttcc catttaagcc aaagtacaac ctgatgatat
    11521 ttgcttcgcg aggcgagagt gtcgataata ctcgttcaat ctctttgcgt agactctcgt
    11581 taagtagccc cttatcaggc atgggagagt cgggagaaag gagtacatcg tacatgcttc
    11641 cttcttcatc ttgagtaatt ggggcatcca ttgataaatg acggttgttg cccttaaggg
    11701 cttccttaac atcatctggt gcaaggttta gcatttcgga tagctcatca atggtaggct
    11761 ccctttcaaa gcgttgctct aactcggcta atgcacggtt taccttgttt atagaaccaa
    11821 tcttgttaag tggcaatttt acaattcgtg cctgttcggc aagggcttgc agtatggatt
    11881 gtctaatcca ccaaacggca tatgatataa acttaaagcc tcttgtctca tcaaagcgct
    11941 gggcagcctt aattaatcca acgttacctt cgttgatcag gtcgggtagg cttaatcctt
    12001 gattctggta ctgctttgca accgatacta caaatcttaa attggctttt acgagttttg
    12061 ccagtgcttc ctggtcgcct tctttaattt tcctggcaag cgttacctcc tgttcggctg
    12121 ttagcaaatc aacctttcca atctcatgaa ggtacttgtc aagcgagaca gcctctcggt
    12181 tagtaacctg tttaattatc ttcaattgcc tcatgccaat gattgatttt taaacattcg
    12241 aatatattaa caaaaattaa aaattgttct ttttgataaa ttttttttat acttcgatta
    12301 aacatttgcg aaaacttatt gtctaaataa atttttctta tggtgtatgg catatttatg
    12361 gtgactaatg ataattcaaa tagtagtatc ttcgtaattt aagttagtaa ctatgcattt
    12421 ttctgttatt ataccggtat ataatcgccc tggtgaggta gaggaattac ttcaaagcct
    12481 tgcaggtcag ggatataata gttttgaggt aataattgtt gaggatgggt caactgttcc
    12541 ctgtagcgat atagtaaaca aattcgcctc tgagttaaaa attaattatt acttcaagga
    12601 aaacactggc ccagggctaa cacgaaacta tggtgccgaa agggcaaaag gggagtactt
    12661 aatctttttt gactccgatt gcataatccc taacaattac tttacccgtg ttgaggagga
    12721 acttgcggca gaatatgttg atttttttgg gggccccgat gccactcacc cctccttcac
    12781 caatgttcaa aaggctattg gctactccat gacctcattt tttacaactg ggggtattcg
    12841 agggggaagg gtaaagctgg agcgttttac tccacgtagc tttaacatgg gcattaatgc
    12901 cgaagttttc cattcggttg gtggtttttc aaacatgagg tttggtgaag atgtggattt
    12961 aagtttaaga cttttcgaga agggttttac gagcaggctt ttccctaaag cctttgtata
    13021 ccataaacgc agaaccgact tccgaaagtt tttcaaacag gttttcaact cgggtatggc
    13081 tcgcataaat ctaagcaaaa atcataaggg ctcacttaag atggttcacc ttttaccctc
    13141 gggatttgtg ttggtaatga gtgcagctat tctgctttca tgggctttcc ccattatatt
    13201 aacgttgccc ttgctataca cttttgcaat aatgttcgac tccttgttaa aaggaaattc
    13261 gctcacggtt gcaatattaa gcatagtggc agcatgggtt cagctatacg gttatggctt
    13321 aggttttatt tatgctacct ggttatgggt tgttcatggt aaaaggccca atggtgcttt
    13381 tactaaaaac ttttaccggt agctatttta tcatttccag caacttgttg cggttaagaa
    13441 tggttatttc acgacggttg atatgaataa ttccctcatc ctgcatctcg gagaaaaccc
    13501 ttgccaaggc agggcgcgtt acgccaaaaa actctgccaa ttcctgatgc gattttggta
    13561 gaatgagcgt ggttctaatt tcgctttttg ataggtttaa caggtagtgc gctatttttt
    13621 ccttaatggt tttaaatgaa agaaaccaaa gcttgtttga cagaaattga gcccggtttg
    13681 atatggagtt aaggtaattg gttagcacct ttgcgttttt ctgcatcagc ccaagaaact
    13741 ccacttgagg gatatacagc agtttgcagt tttccaaggc aataacatca acagggtacc
    13801 tgttgttttc gccaaaaaga aaagcgtggg caattggcat tggagcatgc atctcctcaa
    13861 tttttagaat ttttccagaa aagtcaacca tttcgccctt tacgctcccc tcaactaaaa
    13921 tgcacaaatt tcggacctct tcggttcgct gggctatggt ttgcccttta ctgaatgagc
    13981 gaatggtgtg gggtgtttca tttagtagat gggctacttc attctcagtt aatccccgaa
    14041 atatgggtga taatgctata accttgttca taattttctt aatttgccac aaattaaaca
    14101 aatcaacagg tagtttgctt aataaatatg cctgttaaat atcaaaacgg acaggtggta
    14161 tgcgtacagg agcataaacc tattgctttc aacccttgga agcaccattg tggttttgta
    14221 tgcaatcaaa ttcagctaat cagaggggaa ggaggtggca agctaaatga gcttttaggg
    14281 tatattggag ggactctgct cgatttttac attggtgagc ttcctgttta cacaatagta
    14341 aatgaggtta agaaatattt tatggatttg ggtgtagcca atgttgatga ttttaaacac
    14401 tggcttggct tacctgaaac tacgttcaaa agcatttatc taagcgatgg tcagtgctgg
    14461 gttattcgat taggacaaaa cgatgaatat tacatccaca tacacccggg gcgacactct
    14521 aagctgacta tccgctgtag gccaacaact ctaaaaactg ccattgcttt caggtacctt
    14581 tttggatttg aggatgaaag tttggaaatt gacaaactca attatgcccg taagcttgca
    14641 ggtttacccc cggttgaatc gttaaattca gcttcggctt tgcaaaattt tatatccaac
    14701 ctaagggtta ataatcgtta aaaaatcaca tgtgttttaa aacatatact gctgaatatc
    14761 agtatataca agccattcaa aaaaggtgtt ttatatcatc gtttttggcc tgaattttgt
    14821 gaaactatgg ttgtattttt gcgttataaa agtagttttt caagtatagg ttttagggtt
    14881 aaaagttggt taggtttagt tgatttaggt tagttagggt gtaaggacgg agccgtattt
    14941 tcggttccgt ctttattttt acacggattt atgtcctaaa gcaccaaaca attccataaa
    15001 attcgtaggt ttgagtgaaa aatttagcgt atgaatctgt ccttatccaa agcattcagc
    15061 ttcatatcat atttattgat aatatcctgc tcaactgaaa ctgaaaggtt aaccacaaat
    15121 gtattcaggt ataacgagag taaaggaata acctcgcttg accccgctta tgcccgtaat
    15181 cttgcattaa tttggccggt gagccagata ttcaacggtc ttgttcagtt ttccgattcg
    15241 ctaactgttg agccttccat tgcaaagcgt tggtctattt cccccgatgg gttaacctac
    15301 acatttttat tacgcaacga tgtatatttc catgattcac ctgttttcaa atcaggcaaa
    15361 gggcgacgcg ttacagccca agattttgtt tactcctttg gccgaatact caaccccaca
    15421 acagcttcgc ctggaatgtg ggtgttaagc gtgctcgata aggagaaccc aaacta
//
LOCUS       DAOSAL010000063        15459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_2618_length_15459_cov_51.238380, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000063 DAOSAL010000000
VERSION     DAOSAL010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 15459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15459
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2618_length_15459_cov_51.238380"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="PK049_08840"
     CDS             <1..714
                     /locus_tag="PK049_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61706.1"
                     /translation="GAVNVKTCAITFQNNTIDYAKTYGILVNSEGSFTSITNNTFSNC
                     GDHPLRMSAKYMHTIGTGNVFNCLADKGVNIYAGDATGNITWRKLDKPYYIEGEIDFD
                     NGTLTIEPGTILKFDANGSFHIGYYNNSTLIANGNSSNYIEFTSSASSPAAGAWDCVH
                     LWDNNTSNTSFQYCKFKYAGKGSSSDRAALKASSTSFSLNNCEFSYSGGWGIYLFSST
                     YTGSGNTFTSCTLGDVGHN"
     gene            823..2142
                     /gene="gldE"
                     /locus_tag="PK049_08845"
     CDS             823..2142
                     /gene="gldE"
                     /locus_tag="PK049_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated protein GldE"
                     /protein_id="HOK61707.1"
                     /translation="MESFSSFLNEIAQGVIVKTPSLEFYFGLLVLIILLFLSALFSGS
                     EVAYFSLTPKDFKELESRKKQSKTLKNISHPELLLATLLVANNLVNVAIVILSTFITL
                     SVLDFSHAPLVGFLFQTVIITFILLLFGEILPKILASNRSLQFAERFAPFIGLLISMF
                     KPITKLLIKSSVVISRRMATPQKISMDELGDAIELTSDGLAEERQILEGIVSFTSTMV
                     SEIMKPRLDVVALDYNTSYSRLKEVVIESGYSRIPIFNESFDDIKGILYVKDLIPHVD
                     KSDDFSWQTLIRPPYFVPESKRINDLLTEFQQKHIHLAIVVDEYGGTSGIVTLEDILE
                     EIVGEIADESDEEERLYTKVDDNNYIFEAKVMLNDFCKIVGCDDDIFDDIRGEAETIA
                     GLILENTGQIPKVNSVVKCKQFTFTIDAVDNRRIKRVKVNISPNENN"
     gene            2129..2704
                     /gene="gldD"
                     /locus_tag="PK049_08850"
     CDS             2129..2704
                     /gene="gldD"
                     /locus_tag="PK049_08850"
                     /inference="COORDINATES: protein motif:HMM:TIGR03512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldD"
                     /protein_id="HOK61708.1"
                     /translation="MRITKVRLIFLLLFVVLVACTESPVPKPRGYFRISFPEKQYRLF
                     DTIYPFTFEYPVYGSIVKEENRQGEGEWISVSFPAYKAKIHLSYKDISGNLSQLTEDA
                     RNLAYKHTYKADAIDEQLFSNPDKRVYGILYDIKGNSASSVQFYVTDSSRHYLRGALY
                     FRCQPNKDSLAPAIDFFKADVIHLIETIRWK"
     gene            2712..3350
                     /locus_tag="PK049_08855"
     CDS             2712..3350
                     /locus_tag="PK049_08855"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HOK61709.1"
                     /translation="MPKANSISHDDIRIATWLITESVEELITLTGFSAPLPKSESRQR
                     EQLATAALLQSEGLPASYSYDQEGRPIIPESNVFISITHTDGLVAIAYSTNQPVGVDV
                     EHLGRDFKRVSGKYLTEHESLQSIQYSDEHFALTWCAKESIYKLPWPKSLVFSRDIEV
                     IFESETLKRCWLFAKVQNGTGWKTIKVFFTFINQYCLTWVGMNSILNGNAIR"
     gene            3331..4023
                     /locus_tag="PK049_08860"
     CDS             3331..4023
                     /locus_tag="PK049_08860"
                     /inference="COORDINATES: protein motif:HMM:NF003198.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HOK61710.1"
                     /translation="MGTLYDKIVKGGLAILLDPDKESQVDFDAIVANGQRAGASMFLV
                     GGSLVSKPLDSFVSNLKGKTSLPVVLFPGSAMQFTPVADGILFLSLISGRNPDFLIGH
                     HVTVAPMVKQSGVEVIPTGYILIDGGAATSVQYMSQTMPIPASKPDIAVATAIAGELL
                     GLKAIYLEAGSGAKNEVPAKVIEAVRKAISVPLIVGGGIRSKQQFTDALSAGANLVVV
                     GNGLETNPRMFG"
     gene            complement(4024..5673)
                     /locus_tag="PK049_08865"
     CDS             complement(4024..5673)
                     /locus_tag="PK049_08865"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOK61711.1"
                     /translation="MRLSVFISTILVLGLIETSTAQLNRSIPSEKPKLIVQLVVSQMR
                     FDYISRYWDKFGDNGFKLLVNEGTYCRNARYNYLLTQAYPGLATIATGTNPSVHGIIA
                     DKWFNRNTGNEVEATADDKVNTVGGSFFSGKYSAKNLVTSTFGDELKLNNANSKVVSI
                     ALDAGSAILLGGHSANGMYWFDTEKGLWVSSSYYTDALPAWVDTFNSKGFAKLYTDRE
                     WKALQPIQKYEEADTAAVKSVEKKISLAEKLKGMMDGIIGKPQPKTDFKVLLENPYGN
                     LLTKDLAIAAVVGENLGADEHPDLLCITFSANRFIGGKFGPHSIEMEDTYLRLDRELA
                     HFLEFINSTVGKENCLFILTSDQGVASTPDYLEKSKIPGGYFDPKKAMILLGSYLNAL
                     YGQGNWITGYHDKQIYLNRKLIEDSNLNLADFQQKVADFMLEFSGVSNSTTAHNLQNG
                     NFANGIMVKFQNSYNQRRSGDVIINLEPGWVERNGKVTSANSPYDYDSHVPLIFYGWK
                     AKRKSVLNPVYINDLAPTLSILLGITWPNGATGTPIKEMIE"
     gene            complement(5687..8251)
                     /locus_tag="PK049_08870"
     CDS             complement(5687..8251)
                     /locus_tag="PK049_08870"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOK61712.1"
                     /translation="MAFNKTLLLFFITGLPCFVFPQNNEYYFRFLESNRNKVNTKITQ
                     TVSIDNIKGDTIYAYANQQEYDAFLKLGYKVEVLPHPSNLSAKAVVMATTIEEMAGWD
                     RYPTYEVYRAMMKKFETVYPTLCKLDSIGTTVQGRKLYILKISDNVESNEPEPEVFYT
                     STMHGDETTGYILLLRLIDYLLSNYNALPQVKELVDNLQIYINPNANPDGTYYGGNST
                     VVNARRSNANGIDINRNFPDPRAGQHPDNNQWQPETQAMMNFATQHQLVLSANFHGGA
                     EVVNYPWDTWTSTQEKHPDNDWFVTLSRAYVDTVHKYSPSTYMDGFENGITNGGDWYV
                     VTGGRQDYMNYYRRCREITIELSNTKLLGSELLPTLWNYNSASLINYLKSALYGFGGT
                     VKNTSNEPIDAEVIILNRDRLNSSVKTNPSNGQYFRLLEPGTYQIVAIANGYKPSLIE
                     QLTVSANEYHTVNFTLEPDNEVQATESFEQGVPERLTFTNGTWIASNTAANTGAYSLK
                     SPSTGNSQSTECILNIDVRKPGLVNFYHKVSSETGYDFFKFYIDGKLQGIWSGISNWE
                     RFTTALDVGAHLLKWVYIKDGDGTAGSDCVFIDDLSLPKCNGQINLTTTINSNAYENL
                     KIVLGDSIRTTNTSGMVAFLNYPLDTTVNLEAYSESNLLGSNQVELSWQRVNYWANLS
                     SHFQVTFEVSSKGSPLQNAIINFNGEQKQTDLNGRALFSNVPFGLQHPYSAIKDGYNP
                     ASGTVRVASDTLYSISTYPTNVDTKPFEECFSVYPNPIEGNFWVDIALTERTFIQVKL
                     TDLTGKTIAYIHTGNANPGVTRFFGDSKAMNIQPGIYIFAVDCKKFSLRKKVVFIN"
     gene            8424..8600
                     /locus_tag="PK049_08875"
     CDS             8424..8600
                     /locus_tag="PK049_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61713.1"
                     /translation="MVNLNVKANNIRKGLSGFLHIENLLNTEYYGQTINANWGSPKIL
                     QDLRRIDFGIEIRF"
     gene            complement(8609..8965)
                     /locus_tag="PK049_08880"
     CDS             complement(8609..8965)
                     /locus_tag="PK049_08880"
                     /inference="COORDINATES: protein motif:HMM:NF021221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HOK61714.1"
                     /translation="MENITYTSKDERTFSMLCHLSALSGLIIPFGHIIGPLVVWLLKK
                     EEFPEVDRQGKAALNFQLSLTIYSIIAGILVIFLVGFVLLGAIFLLGLILTIIASVKS
                     SNGERFEYPLSINLIK"
     gene            complement(9199..10872)
                     /gene="ettA"
                     /locus_tag="PK049_08885"
     CDS             complement(9199..10872)
                     /gene="ettA"
                     /locus_tag="PK049_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HOK61715.1"
                     /translation="MADEKIIFSMVGVSKTFPPHKKVLNNIYLSFFYGAKIGIIGLNG
                     SGKSTLMKIIAGVEKSYEGEVVFSPGYTVGYLEQEPQLDENKTVREVVEEGVQEVVDL
                     LKEYEAVNARFAEPMSDDEMNKLIEKQGELTDKLEHLDAWNLDSKLERAMDALRCPEG
                     DTPVKVLSGGERRRVALCRLLLQQPDVLLLDEPTNHLDAESVQWLEMHLQQYKGTVIA
                     ITHDRYFLDNVAGWILELDRGEGIPWKGNYSSWLEQKSLRLAQEEKQESKRRKTLERE
                     LEWVRMSPKARHAKSKARLSAYDKLLNEDVKQKEEKLEIFIPNGPRLGDLVIEASNVS
                     KAFGDKLLFENLSFKLPPNGIVGVIGPNGAGKTTLFRLIMGLEKPDSGEFRVGETVKL
                     AYVDQQHKAIDPDKTVFEVISGGADNILLGGRQINARAYVARFNFSGADQEKKCGMLS
                     GGERNRLHLALALKEEGNVILLDEPTNDIDVNTLRALEEGLEDFAGCAVVISHDRWFL
                     DRIATHILAFEGNSQVYFFEGTYSEYEENKKKRLGDDTPHRIKYRKLME"
     gene            complement(10952..11797)
                     /locus_tag="PK049_08890"
     CDS             complement(10952..11797)
                     /locus_tag="PK049_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61716.1"
                     /translation="MKKLSLIIGLVTITLWGMAQRNLVRPEDVKAFMASTTYVVLENN
                     PTSQFNIEIREAVERSWKITPYKFITAKEFNDMRKDIDKSFLVLIQMRFDKDKIAPTY
                     NFLSVSMGAAVSKITDMPDICSVPLSYKDLSEDTYTYKLEGIIRFMQNYITAINENPK
                     LIKKDAFKFYNKNMKQVKSKELWVTESALDKTVRPLAEIRKVYKFNIKVVNPEDIQKA
                     IQEKNPNVLYLHKVGPEGSRLQWRVWKMILGAGDDQLYYYDMHTISKSKGDGFLSKDF
                     AKINK"
     gene            12048..13886
                     /locus_tag="PK049_08895"
     CDS             12048..13886
                     /locus_tag="PK049_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOK61717.1"
                     /translation="MTESRNKIVNADEIKKAVNLGPIIGDGFARLLMYILRFNELNKK
                     YANVANLQGLEFLNAALDILEVKFEVNPEELKRIPKNGAFISVSNHPFGGMDGILLLK
                     IMLEQQRDDIRLLTNFLIKRIPQVEKFVFAVDPFENGGYQKNSNISGLKAAYTHLSEG
                     GALGLFPAGEVSTYYDNVDETGVTDRHWQRSMIKFIKNARVPVVPIYFQGENSRLFHL
                     LGLIHPLLRTAKLPSELLNKKNQTIRVRIGYPISVEEQDNYCDIEKFGRFLRVKTYSL
                     GSTIDVDKFFKYKLKSEPEPEPIIPPVPQEVIEDEVSKLIENYLLFRSKNYAVICAPS
                     VEMPNLMTEIGRLREITFRDVGEGTNRKIDVDEFDLYYWQLFIWDEDERRIVGAYRAG
                     KGKEIIEKYGIEGFYIQTLFKIDKAFAPILEQCIELGRSFIVPDYQRKPLPLFLLWKG
                     ILYFLIKNPEYRYLIGPVSISNRFSDYSKGVIISFMKSNYYDHDFARFIKSRNKFKVP
                     IKDEEFDIIFNNSDDLGKLDKFIQEVEPYDYRMPVLLKKYVKLNGKIIGFNVDPKFNN
                     ALDGLLILDLFQVPAETITSLSKEINDKSILERFNITDYTFEDQAE"
     gene            13981..15207
                     /locus_tag="PK049_08900"
     CDS             13981..15207
                     /locus_tag="PK049_08900"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HOK61718.1"
                     /translation="MNRYFLIFLAFAFLGCSNRTVTDDAKLEQRAKALHSKMVTIDTH
                     TDTPLNFLRTGFDFTGQNNTAIGSKVDLAKMEQGGLDAAFFAVFIGQRECTPEMYAQV
                     NAKAKEIFDAIYTTVNKHSNRASIATKPDDVYRLKKEGKRAIYIGVENGYPIGDDLSQ
                     VKLFYDMGARYITLCHTRNNQICDSSTDPDGPRHNGLSQFGREVVAEMNRLGMIVDVS
                     HISDKAFYDVLEVSKAPVIASHSCSRAICDNPRNLTDDMLRSIARNGGVVQMCILSEY
                     VKKGEPNPARDSAYRALRRKWNNFRGLTPEQEKQATTEWYELEEKYPASLATVADVVD
                     HIDHMVKVMGIDHVGIGTDFDGGGGVDGCADASQMYRITMELLRRGYSERDIRKIWGE
                     NFLRVFREVEKRKERG"
BASE COUNT         4222 a         3288 c         3454 g         4495 t
ORIGIN      
        1 ggggctgtta atgtaaaaac ctgtgcaatt acgtttcaga ataatactat tgattacgca
       61 aagacttacg ggatacttgt gaactcagaa gggagcttta ctagtattac aaacaatact
      121 tttagcaatt gtggggatca tccattacgt atgtcggcaa agtacatgca cactattgga
      181 accggtaatg tttttaactg tctggccgat aagggtgtaa acatatacgc aggcgatgca
      241 actggaaaca taacatggcg caagctcgat aaaccttact acattgaagg tgaaattgac
      301 tttgataatg gtacccttac cattgaaccg ggcactatac tgaaatttga tgccaatggg
      361 tcgttccaca taggctatta caacaattcc actcttattg ccaatgggaa ttcaagcaac
      421 tacattgagt ttacttcaag cgcttcatcg ccggctgccg gcgcatggga ttgtgtacac
      481 ctttgggata acaacacctc taataccagt ttccagtact gcaagttcaa gtatgcaggc
      541 aaaggtagct ccagcgatag ggctgcccta aaagcttcga gcacttcttt ttcattaaat
      601 aactgcgagt ttagttactc tgggggttgg ggtatttatc tgtttagctc tacctataca
      661 ggatctggca atacttttac atcctgtacg ctgggcgatg ttggccataa ctaaatttta
      721 acctcttata ttagtttttc caagagtagc gcaaagttaa atactttgcg ctatttatta
      781 tttttgctaa ctttacaggg caaaccaata cccactttaa ttttggaaag ttttagtagt
      841 ttccttaatg agattgccca gggagttatt gtaaaaaccc catcacttga attctatttt
      901 ggcctattgg tgttaattat actacttttt ctctcggcgc ttttcagcgg ttccgaggta
      961 gcctactttt cgttaacacc aaaggatttt aaggagcttg agagcaggaa aaagcaatca
     1021 aaaaccctaa aaaacatctc gcaccccgag ttactcctgg caaccttact ggttgcaaat
     1081 aacctagtta atgtagccat tgtaatactt tctaccttta taaccctatc ggttcttgat
     1141 tttagccatg cacccttggt cggttttctg tttcaaacgg ttataattac tttcatactg
     1201 cttctatttg gtgagattct tcccaaaata ttggcatcaa ataggtcatt acaatttgcc
     1261 gagcgatttg cgcccttcat tgggctgctc atttcaatgt ttaagccaat tacaaaatta
     1321 ctcattaagt cgtcggtggt tataagtagg cggatggcca ctccccaaaa aatctcaatg
     1381 gatgagcttg gcgatgccat agagcttact tccgatggtc tagccgagga acgacaaatt
     1441 cttgagggaa tagtatcgtt caccagcacc atggttagtg agattatgaa accccgactc
     1501 gacgtggttg cacttgacta caacacctcc tatagtaggc ttaaagaggt ggttattgaa
     1561 tcgggctata gccgaatacc catttttaat gagtcgttcg atgatataaa gggaatactt
     1621 tacgttaagg atttaatccc ccacgttgac aagagcgacg atttttcctg gcaaacactc
     1681 atcagaccac cctattttgt accggaaagc aagcgaatta acgacttgct cactgagttc
     1741 cagcagaaac atatacacct tgctatagtg gtagacgagt atggcggcac aagcggaatt
     1801 gttacccttg aggatatact tgaggagatt gttggcgaaa ttgccgatga atccgacgaa
     1861 gaggaacggc tttacacaaa ggttgacgat aacaattaca tttttgaggc caaggtgatg
     1921 ctaaacgact tttgtaagat tgttgggtgc gatgatgata tctttgacga tattcgtggc
     1981 gaagccgaaa caattgcagg cttaattctg gaaaacaccg ggcaaatccc taaggtgaac
     2041 tcagtggtaa aatgcaaaca gttcactttc actattgatg cagtggacaa ccgcagaatt
     2101 aaacgggtta aggttaatat tagtcccaat gagaataact aaggtaagat tgatattttt
     2161 gcttcttttt gtcgtgcttg tggcttgcac tgaaagtcca gttcctaaac cgcgtgggta
     2221 tttccgtata tcgttccccg aaaaacaata ccgattattc gataccattt acccctttac
     2281 ttttgagtat ccggtttatg gttccattgt aaaggaagaa aaccgacagg gtgagggtga
     2341 atggataagc gtgagttttc cggcctataa ggccaagata cacctgagct ataaggatat
     2401 ttcgggcaat ttgagccagt taaccgaaga tgcacgtaac ttagcctata agcataccta
     2461 caaggccgac gccattgatg agcagctttt ctcaaatccc gataagaggg tttatggcat
     2521 tctgtacgac atcaaaggga actctgccag ttcagtgcag ttttacgtta ctgactcaag
     2581 caggcattac ctacgtggag cactttactt ccgctgccag cccaataagg actcccttgc
     2641 cccagccatc gactttttta aagcagatgt gattcatttg attgaaacca ttaggtggaa
     2701 gtagagttct tatgcccaag gctaactcca tatcacatga cgatatacgg atagccacat
     2761 ggctaatcac tgaaagcgtt gaggaactga taacccttac gggtttttcg gctcctttac
     2821 caaagagtga atcgcgccaa agggaacagc ttgcaacagc agccctgctg cagtccgagg
     2881 ggttacctgc aagttacagc tacgaccagg aagggcggcc tattattcct gagtcaaatg
     2941 tttttatctc cattacccac accgatggtc tcgtagccat tgcatactct accaatcaac
     3001 cagttggtgt tgatgtagag cacttaggtc gtgatttcaa aagggtttca gggaaatacc
     3061 ttactgaaca tgaatcttta caatcaattc aatactccga tgagcacttt gccctaacct
     3121 ggtgtgcaaa ggaatccatc tataagctac catggccaaa aagtttagtg tttagtcgtg
     3181 atattgaggt gatatttgaa tccgaaacgc taaaacgttg ctggcttttt gctaaagtac
     3241 aaaatggtac agggtggaaa acaataaaag ttttttttac ctttatcaat cagtattgcc
     3301 ttacatgggt gggtatgaat agtattttga atgggaacgc tatacgataa aatagtaaag
     3361 ggtggccttg ccatactgct cgatcccgac aaggagtcgc aggtggactt tgatgccatt
     3421 gttgccaatg ggcaacgggc tggagcgtcg atgtttcttg taggcgggag tttggtttcc
     3481 aaaccgctcg atagctttgt aagcaaccta aagggaaaaa catcgttgcc tgtggtgcta
     3541 ttccccggaa gtgccatgca gtttacaccc gttgccgatg gtatcctttt tttatcgtta
     3601 atatcgggtc gtaatcccga ttttttgata ggccaccatg taactgtagc cccaatggta
     3661 aagcagagtg gcgttgaggt aatcccaaca gggtacattc taattgatgg gggagccgcc
     3721 acatcggttc aatacatgag ccaaaccatg cccattcccg caagtaagcc cgacatagca
     3781 gtggctaccg ccattgcagg cgaacttctg ggactaaaag ccatttacct tgaggcgggt
     3841 agtggggcaa aaaacgaagt acctgctaag gtgattgagg ctgtaaggaa agccatttcg
     3901 gttccactca ttgttggtgg aggcatacgc agtaagcagc agtttaccga tgctcttagt
     3961 gccggagcta accttgtagt ggttggtaat gggcttgaga caaacccgcg aatgtttggt
     4021 taactactca atcatctcct taatgggcgt tccggttgcg ccgtttggcc aggtaatccc
     4081 aagaagtatg gagagagttg gtgccaaatc attgatgtaa accggattaa gtaccgattt
     4141 gcgttttgcc ttccatccat agaatattaa tgggacatgt gaatcgtagt cgtagggtga
     4201 gtttgccgag gttactttcc cgttacgctc aacccatccg ggttcaaggt tgatgattac
     4261 atcgccggag cgacgttggt tgtagctatt ctggaactta accatgattc catttgcgaa
     4321 attgccgttt tgtaggttat gcgcggtggt gctattggaa acgccgctaa attctagcat
     4381 aaaatcagca accttttgct ggaaatcggc aaggttaagg ttggaatcct caataagttt
     4441 acggttcagg tagatttgtt tatcgtgata gccggtaatc cagtttccct gtccgtagag
     4501 tgcattgagg taacttccta gtagtatcat agcctttttg gggtcgaagt aaccaccagg
     4561 aatcttcgat ttttcaaggt aatcgggggt tgaggctaca ccttggtcgg aggttagtat
     4621 aaaaagacag ttctccttac caacagttga gttgataaac tcaaggaaat gggcaagttc
     4681 cctatcgagg cggaggtagg tatcctccat ctcaatggag tgtgggccaa atttgccacc
     4741 aataaatcgg ttagcgctaa aggtaatgca aagcaagtcg ggatgctcat cggccccaag
     4801 attttcaccc actaccgctg ctatggcgag gtccttggtg agtaggttgc cgtaggggtt
     4861 ttcaagtaaa accttaaagt cggttttggg ttggggtttt ccaataatgc catccatcat
     4921 accttttagc ttttcagcta aacttatctt cttctcaact gactttacag cagcggtgtc
     4981 ggcttcttcg tatttctgaa ttggctgtaa agccttccat tccctgtcgg tatagagttt
     5041 agcaaaacct ttgctattaa aagtatctac ccacgccgga agtgcgtcgg tgtagtagct
     5101 gctgctaacc cataaacctt tttcggtatc gaaccagtac ataccatttg cgctatgccc
     5161 acctaaaagg attgccgacc ctgcatcaag ggcaatgcta actaccttgg agtttgcatt
     5221 attcagcttt agctcatcgc caaaggtgct ggtgaccaaa ttctttgcag agtatttacc
     5281 gctaaaaaag ctgccaccaa cggtattcac cttatcatcg gcagttgcct caacctcatt
     5341 tcctgtattg cggttaaacc atttatcggc aataattcca tggaccgatg ggttagttcc
     5401 tgtggcaatg gttgccaaac ctgggtaagc ttgagtaagt aggtagttat aacgagcatt
     5461 gcggcagtag gttccctcgt taacaaggag tttgaacccg ttatcgccaa acttatccca
     5521 gtagcgcgag atgtaatcga atcgcatttg gctgactact agttgtacta tgagctttgg
     5581 cttttccgag ggtatggaac ggtttagctg tgcggttgag gtttcaataa gtcctaatac
     5641 caagattgtt gaaataaata ctgaaaggcg catggttaaa tcgggtttaa ttaataaaaa
     5701 ctactttttt acgtaaagaa aactttttgc aatcaaccgc aaaaatataa atgccgggtt
     5761 gaatattcat tgccttgcta tcgccaaaaa atcgtgttac tcccggatta gcattgccgg
     5821 tatggatata ggcaatggtt ttacccgtta aatcggtaag ttttacctga atgaatgttc
     5881 tttcagtaag tgctatatca acccaaaaat ttccttctat agggtttggg taaactgaaa
     5941 aacactcttc aaatggtttt gtatcaacat ttgtggggta agtggagatt gaatataggg
     6001 tatcgctggc tacccttacc gtgccagagg cggggttata cccatcttta attgctgagt
     6061 atgggtgttg taaaccaaat ggaacattgc tgaatagtgc tctaccgtta aggtcggttt
     6121 gtttctgttc tccattaaag ttgatgattg cgttttgcaa tgggcttccc ttagagctaa
     6181 cctcaaaagt gacctggaag tgagagctga ggttagccca gtagttaacc ctttgccacg
     6241 ataattcaac ctgattgcta cctaaaaggt tgctttcgga gtaggcttca aggtttacag
     6301 ttgtatcgag cggatagttc aagaatgcaa ccatcccgct agtgttagtt gttctgatgc
     6361 tatcgccaag gacgattttt agattctcgt aagcatttga gttgatagtg gtagttaggt
     6421 ttatttggcc attgcacttg ggtagcgata aatcatcgat aaaaacgcag tcggaacctg
     6481 ccgttccatc cccatccttt atgtataccc acttaagcaa atgcgctcca acatcaagcg
     6541 ctgtagtaaa cctttcccag ttgctaatac cactccaaat gccttgaagc ttaccatcaa
     6601 tataaaactt aaaaaagtcg taacctgttt cggaagaaac cttatgatag aagttaacta
     6661 gtccgggttt tctgacatcg atgtttagga tacattcggt tgattgcgag ttaccagtag
     6721 agggcgattt taagctatag gctccagtgt ttgctgcagt attacttgca atccaggttc
     6781 cattggtaaa tgttaaacgt tctggtacac cctgttcaaa actttcggtt gcttgaactt
     6841 cgttgtcggg ctctagtgtg aaatttaccg tatggtattc atttgccgat accgttagct
     6901 gttcaattaa gcttggtttg tagccgtttg caatagctac aatttggtag gtacctggtt
     6961 ctaacaatct aaagtactgt ccgtttgaag ggttagtttt aacggatgag tttagcctgt
     7021 cgcgattcaa tattattact tcagcatcga tgggctcgtt cgaggtattc ttaacagttc
     7081 ctccaaaccc ataaagggca ctttttagat agttgataag cgaagcgctg ttgtagttcc
     7141 aaagtgttgg tagcagttca gagccaagta gttttgtatt ggataattca atggtaatct
     7201 cgcggcagcg gcggtagtaa ttcatgtaat cctgccttcc accagtaacc acataccaat
     7261 cgcccccatt ggttattcca ttttcaaatc catccatgta tgttgatggg ctgtatttgt
     7321 gaacagtatc aacgtatgcc cttgaaaggg ttacaaacca atcgttatcg gggtgttttt
     7381 cctgtgtcga cgtccaggta tcccaggggt agtttaccac ttcagcaccg ccatggaagt
     7441 ttgccgaaag cactaattga tgctgcgtcg caaagttcat catggcctgt gtttccggtt
     7501 gccactggtt gttgtcgggg tgctggccgg ctcgcgggtc agggaagttg cggttgatat
     7561 caatgccgtt agcattggaa cgccttgcat tcacaacggt tgaattaccg ccgtagtacg
     7621 ttccatcggg gttggcatta gggttgatat agatttgcag gttatcaacc agttccttaa
     7681 cctggggcag ggcgttgtag ttcgatagta ggtagtcaat aagcctaagc agtaggatat
     7741 atccagtggt ttcatcgcca tgcatggtgc tggtataaaa gacctcgggt tcaggctcat
     7801 tgctttccac attatcggaa atttttaaaa tatatagttt gcgtccctga acggtagttc
     7861 caatagaatc gagcttacag agggtagggt agactgtttc aaattttttc atcatggccc
     7921 tgtaaacctc ataggtggga tacctatccc atcctgccat ctcctcaatg gttgttgcca
     7981 ttacaacggc cttggcactt aggttcgatg gatggggaag cacttcaacc ttatacccta
     8041 actttagaaa agcgtcatat tcctgctggt tggcataggc ataaatagta tctcccttga
     8101 tattatcaat tgatacggtt tgggtgattt tggtgtttac cttgttgcgg ttagactcaa
     8161 gaaacctaaa gtaatactcg ttgttttgtg ggaaaacaaa acagggcaaa ccagtaataa
     8221 aaaaaagaag gagggttttg ttaaatgcca tattaggtga tttttgatgt tgtaaactta
     8281 agtaaaattt gatagatttg agtgaaatcc ttaagtcaaa atgaagtgta atatcaacaa
     8341 aatcaacctg aaacttttga gcaattatat tctataacaa ctgccaacgt tatgggctat
     8401 ttgttaattt cgatccagtt cttttggtaa atcttaatgt aaaagcgaat aatatccgga
     8461 aaggactatc gggtttcctg cacatcgaga acttactcaa caccgaatac tatgggcaaa
     8521 ccataaatgc aaactgggga agccctaaaa tacttcagga cctccgcaga attgattttg
     8581 ggattgagat taggttctag ccgacttttt actttatgag atttatggaa agtgggtact
     8641 caaaacgctc gccattcgac gattttaccg atgctataat ggttaaaata agccctaaaa
     8701 ggaagatggc acccagtagc acgaaaccaa ccaaaaagat tacaagaata cctgctataa
     8761 ttgagtaaat ggtaagcgat agctgaaagt ttaacgctgc cttaccctgc cggtcgacct
     8821 cagggaattc ttcttttttt agtaaccaaa caacaagtgg cccaataata tgcccaaaag
     8881 gaattattaa tcccgagagt gctgaaagat gacagagcat gctgaaggtg cgctcatcct
     8941 tacttgtgta agtaatgttt tccataatga aagtatttat accaaaatta cgaaaagtta
     9001 gaacgatttg ctgcatttgt aataaaaatt gcatttaaat gttggattaa tagaattttg
     9061 acatcatgtt taatgtgaaa aaagtttatt aaccttaaag gcttaattat tctaagtagc
     9121 cctgttaaag ttgaagttaa ctaaatgcag aatgcctaat tcacaaaaaa aataccggcc
     9181 ttaaagccgg tatttagttt attccattag cttgcggtac ttaatcctat ggggtgtatc
     9241 gtcgccaagc cgtttctttt tgttttcctc atactctgag taggttcctt caaagaagta
     9301 aacctgggag ttaccctcaa aggcaagtat gtgagtagca atacggtcga ggaaccaacg
     9361 gtcgtgcgag attaccacgg cgcagccggc aaagtcctcc aatccttcct caagggctcg
     9421 cagtgtgttc acatcgatat cgttggtagg ctcatcgagc agaattacgt ttccctcctc
     9481 tttaagggca agagcaaggt gaagcctgtt ccgctcccca cccgatagca tgccacattt
     9541 cttttcctga tcggcaccgg aaaagttgaa acgtgccacg tatgcgcggg cgtttatttg
     9601 acggccgccc agcaagatat tatcggcgcc gcccgatatc acctcaaata ccgttttatc
     9661 ggggtcaatt gctttgtgtt gctggtcaac ataggctagc tttacggttt cgcctacacg
     9721 gaattcgccg ctatcgggct tttcaagccc cataataagg cggaagagtg tggttttacc
     9781 tgcaccgttg gggccaataa cacctactat tccgtttggt ggaagcttaa acgaaaggtt
     9841 ttcgaaaagc agcttatcgc cgaaagcctt ggaaacgttg ctagcttcaa ttaccaaatc
     9901 gccaagtcga ggcccgttgg gaatgaaaat ttcaagcttt tcttccttct gttttacgtc
     9961 ctcgttcagg agcttatcgt atgccgagag gcgggctttt gacttggcat gacgggcttt
    10021 aggcgacata cgcacccatt ccagctcgcg ctcaagggtt ttacggcgtt tgctctcctg
    10081 cttctcctcc tgtgccaggc gtaacgattt ttgctccagc catgaggagt agttcccttt
    10141 ccagggaatt ccttcgccac ggtcgagttc caggatccaa cctgcaacgt tatcgaggaa
    10201 gtaacggtcg tgggtaatgg ctataacagt tcctttgtac tgctgtaggt gcatctcgag
    10261 ccactgaacc gactctgcgt ccagatggtt ggttggctca tccagcaaca gtacatcggg
    10321 ctgctgaagc agaagcctac aaagggcaac ccggcggcgc tctccccctg atagcacctt
    10381 aacgggtgta tcgccctccg ggcaccggag agcatccata gctctttcaa gtttcgagtc
    10441 caggttccag gcatccagat gctctaactt gtcagttagt tcaccctgtt tttcaatgag
    10501 cttgttcatc tcatcgtcgc tcataggctc tgcgaagcga gcgtttaccg cttcgtattc
    10561 cttgagcagg tcaactactt cctgaacccc ctcctcaaca acctcacgga ctgttttatt
    10621 ttcatccagt tgaggttcct gttccaggta gcccactgtg tagcccggtg aaaataccac
    10681 ttccccttcg tacgatttct caacccctgc aataatcttc atcagggtcg atttacccga
    10741 accgtttaaa ccaataatac caatcttagc gccgtaaaaa aacgacaggt agatgttgtt
    10801 cagcactttc ttgtggggag ggaaagtttt actcaccccc accatggaaa agataatttt
    10861 ttcgtcggcc attgtgttta cattttcagc tgcaaaaata accgccggca ggcaaatacc
    10921 cgtcggcggt aaaaaatatt ttcagattct actacttatt gatttttgca aagtctttgg
    10981 ataggaaacc atcgcctttt gatttggata tggtatgcat atcgtagtaa tagagttggt
    11041 cgtcgccggc gcccagaatc attttccaaa cacgccactg caggcgactg ccttccgggc
    11101 caaccttatg caggtaaagc acatttgggt tcttttcctg gatagccttt tgtatatcct
    11161 ccgggtttac aaccttgatg ttaaacttgt aaaccttcct aatctcagca agaggacgaa
    11221 ccgttttgtc gagtgcgctc tctgttaccc aaagttcctt actttttacc tgtttcatgt
    11281 tcttattgta gaacttaaag gcatccttct tgatgagttt aggattctca ttaatggcgg
    11341 tgatatagtt ttgcatgaaa cggataatac cctcaagctt gtaagtgtat gtgtcctcgg
    11401 ataaatcctt gtagctaagt ggaacggagc agatatcggg catatcggta atttttgaaa
    11461 cggcagcacc catcgatacg ctcaggaaat tgtaggtagg ggcaatcttg tccttgtcga
    11521 accgcatttg gattagcacc aagaatgact tgtcgatatc cttgcgcata tcgttaaact
    11581 ccttagcggt aatgaacttg taaggggtga ttttccatga acgttcaacg gcttcacgga
    11641 tttcgatatt aaattgcgat gtaggattgt tttcaagtac cacataggtt gttgaagcca
    11701 taaatgcctt tacatcctcg ggcctaacca ggttacgttg tgccatgccc catagggtta
    11761 tggttaccag tccgataata agtgatagct tcttcatgat catctatttt tatggttttt
    11821 gtgtttttct attgccattc acaaatttaa tacacttttc aatattgata ggttcaaatc
    11881 tgaaaattcc ttcaataacg acttaatatt tttaaaatca ataaataaat gattgtccct
    11941 gataggttta taggtaattt agaatctaaa aaatgttttt tagcattttt tcttaccttt
    12001 gcgcatggtg gaaacgatat agcatggttt aacctaacac aattccaatg acagaaagta
    12061 gaaataagat tgtcaatgcg gatgagatca agaaagctgt aaaccttggc ccaatcattg
    12121 gtgatgggtt tgcccgttta ctgatgtaca tactccgatt caatgagctt aacaagaagt
    12181 acgctaacgt ggcaaacctt caggggcttg aatttctcaa tgcagcgctt gatatccttg
    12241 aggttaagtt cgaggttaac cccgaggagc taaagcgaat tccaaagaat ggagcgttta
    12301 tttcggtttc gaaccacccg tttggtggaa tggatggtat tttgctttta aaaattatgc
    12361 ttgagcagca gcgcgatgac attaggctgc tcacaaattt cttgatcaag cgtattcccc
    12421 aggttgaaaa atttgtattt gcagttgacc cctttgaaaa tggtggttat caaaaaaatt
    12481 ccaatataag cgggttaaaa gcggcttaca cacacctatc ggagggtggt gcacttggtc
    12541 ttttccctgc cggcgaggtg tcaacctact acgacaacgt agatgaaacc ggagtaacag
    12601 acaggcattg gcaaagatca atgattaagt tcatcaagaa tgcacgcgtt ccggttgttc
    12661 ccatttactt tcagggcgag aatagccgcc tgttccactt gcttggacta atacacccgc
    12721 tactccgtac tgccaagtta ccttcggagt tacttaacaa aaagaaccaa accatcaggg
    12781 tgcgtatcgg atacccaatt agcgttgagg aacaggataa ctactgcgat attgagaaat
    12841 ttggtcggtt ccttagagtt aaaacatact cgttgggatc aaccatcgat gtggataagt
    12901 tctttaagta taagctaaaa tcggaacctg aacccgaacc tatcattcct cctgttccac
    12961 aggaagtgat agaggatgag gtttcaaaac tgattgaaaa ttacctgttg ttccgtagca
    13021 aaaactatgc ggttatttgc gccccttcag ttgagatgcc taacctgatg actgaaatag
    13081 gtcgcctgcg cgaaattacc ttccgcgatg tgggcgaggg tacaaatcga aaaattgacg
    13141 tggacgagtt tgacctctac tactggcagc tatttatttg ggatgaggac gagcgacgta
    13201 ttgttggtgc ataccgtgcc gggaaaggga aagagataat tgaaaagtac ggcattgaag
    13261 gattttacat ccaaacgctt tttaaaattg ataaagcctt tgccccaatt cttgagcagt
    13321 gcattgagct tggccgttcg ttcattgtgc ccgattacca acgcaagccg ctaccgttgt
    13381 tcctactctg gaaagggatt ctttacttcc ttattaaaaa cccggagtac cgctacctta
    13441 ttggccctgt aagtatatcg aaccggttct ctgattactc caagggtgta attattagct
    13501 tcatgaaatc gaactactac gaccatgact ttgcccgatt tataaaatcg cgcaataaat
    13561 tcaaggtgcc tattaaagat gaggagttcg atatcatttt taacaatagc gacgatcttg
    13621 ggaaactcga taagttcatc caggaggttg aaccatacga ttaccgcatg cccgtgctgc
    13681 tgaaaaagta tgttaagtta aatggcaaaa taattggttt caacgtcgat cctaaattta
    13741 acaacgcgct cgatggcctg cttatactcg atcttttcca ggtacctgct gaaactatca
    13801 cttcgctttc caaggagatc aacgataaat cgatactgga aaggtttaac attaccgact
    13861 acacctttga ggatcaggca gagtaaaaaa gaacagaaaa ggcagcaaaa gcgctgcctt
    13921 tttttatgag taaactttta aactaatggt tagtggttta gtctattaaa aaaaatccat
    13981 atgaacagat atttcttaat cttcttagct tttgcttttt tggggtgttc caatcgtaca
    14041 gtaaccgatg atgcaaagct cgaacagcgt gccaaagcac ttcattccaa gatggttacc
    14101 attgataccc ataccgatac ccctttaaat tttctccgta ccggctttga ctttaccggc
    14161 caaaacaaca ccgcaattgg cagcaaggtg gatttggcaa aaatggagca gggtgggtta
    14221 gatgctgcct tttttgcggt attcattggt caacgcgagt gcacacctga aatgtacgct
    14281 caggttaatg caaaagccaa agagatattt gatgctattt acactaccgt taacaagcac
    14341 agtaaccgtg catcaattgc aactaaaccc gacgatgtgt ataggttaaa aaaggagggt
    14401 aaacgcgcca tttacatagg ggttgagaat ggttacccca taggtgatga tctcagccag
    14461 gtaaagctat tctacgatat gggagcccgt tacatcaccc tttgccatac acggaataac
    14521 cagatatgcg actcatcaac ggatcctgat ggcccacgcc acaatgggct aagccaattt
    14581 ggccgcgagg tagttgcgga aatgaaccgc ctgggaatga tagtggatgt gtcgcacata
    14641 tccgataagg cattctacga tgtactggag gtttcaaagg ctcccgttat tgcctcccac
    14701 agctgctccc gcgccatttg cgataatccc cgcaatttaa ccgacgacat gctccgttcc
    14761 attgcaagga atgggggagt ggtacagatg tgtatcctga gcgagtatgt aaaaaagggt
    14821 gaacccaacc ccgctcgcga tagcgcttac cgtgctcttc gtaggaagtg gaataacttc
    14881 aggggcttaa cccccgaaca ggaaaaacag gctacaacgg agtggtatga gcttgaggag
    14941 aaatacccgg catcgttggc taccgtggcc gatgtggttg accacatcga tcatatggtt
    15001 aaagtaatgg gaattgacca tgtgggaata ggcaccgatt ttgatggcgg tggcggtgtt
    15061 gatggttgtg ccgatgcctc gcagatgtac cgcataacca tggagctcct ccgtagggga
    15121 tattccgaaa gggatatccg taaaatctgg ggcgaaaact tcttacgagt tttccgcgag
    15181 gtggaaaaga ggaaggagcg gggataagaa aagcacatat cgttcgaaga atcagatgac
    15241 atctcaccaa ttgggagtca atacctttgt cttcttcact ccctttgtca tgctgagccc
    15301 agccgaagca tctcatttta ctttcattcc ttctattgat attcctcaac caacaaggaa
    15361 taacttaagc attggcataa aagcctgacc cgacaatgag tcgagctttt cagccttgat
    15421 tttctttggt tctttcttgt atcaagacaa gaaagaacg
//
LOCUS       DAOSAL010000064        15336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_2643_length_15336_cov_50.917741, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000064 DAOSAL010000000
VERSION     DAOSAL010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 15336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15336
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2643_length_15336_cov_50.917741"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..839)
                     /locus_tag="PK049_08905"
     CDS             complement(84..839)
                     /locus_tag="PK049_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOK61719.1"
                     /translation="MKALLSHRSIRKYKSDPIDDSLLQEILTAGTRASTTGNMQVYSI
                     IVTRDEEVKRKLLPLHFNQQMVVQAPVVLTFCADFNRFNKWCLQRKANPGYDNFLSFF
                     TAAIDALLVAQNVCIAAEEKGLGICYLGTTTYTADKIIEVLKLPKGVVPVTTVVMGYP
                     DEHPDLTDRLPLAGVVHYDVYHDYSEGDIDQIYQHKESLPLTKRLLEENQKETLAQIF
                     TDKRYTLKDNVAFSKNLLRVLEMQGFMNNERTE"
     gene            complement(936..1889)
                     /locus_tag="PK049_08910"
     CDS             complement(936..1889)
                     /locus_tag="PK049_08910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOK61720.1"
                     /translation="MDISIVVPLYNEEESLPELMEWIAQVMTQNHFTYEVIMVDDGSN
                     DNSWAVIESLRLKFPSLRGIKFLRNYGKSAALQVGFTAAQGDVVFTMDADLQDSPDEI
                     PELYRMVKEQGYDLVSGWKKKRFDPLLTKRIPSKLYNATVRMVSGIKLHDMNCGLKAY
                     RHNVVKSIEVYGEMHRYIPVIARQAGFKRIGEKVVQHRERKYGTTKFGWTRFINGFLD
                     LLSVMFVSRFGKKPMHFFGTLGTLMFITGGAIAVWLVGSKLWAQFHHLKYRPVTDQPL
                     FYIALVVAIIGVQLFTAGFIGELVSRSSADRNHYLIEKELG"
     gene            complement(1905..2450)
                     /locus_tag="PK049_08915"
     CDS             complement(1905..2450)
                     /locus_tag="PK049_08915"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HOK61721.1"
                     /translation="MEKQGNPMLKHSMNYGLIMGVALIILSLITYLAGIVKPPFWVSI
                     INYAIIIGIIVWGTKTYRDEVLEGAITYGNALGFGVLITLFAGVIVAVFTYIQITLID
                     PDFVSKMLAIAEEELVKRGMPDEQIEMAVEMQKKFMTPLIMTISSLLGMVLMGFIFSL
                     ITSIFLKKEKSPFAGGGEVIE"
     gene            2548..2647
                     /gene="ffs"
                     /locus_tag="PK049_08920"
     ncRNA           2548..2647
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PK049_08920"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            2704..3489
                     /gene="truA"
                     /locus_tag="PK049_08925"
     CDS             2704..3489
                     /gene="truA"
                     /locus_tag="PK049_08925"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOK61722.1"
                     /translation="MPRFKLTIEYDGSRYRGWQFQREAATVMGKLMDAIKEVYKVSSY
                     ELYGAGRTDAGVHALGQVAHLDIDSAMPPYVAMERMNEVLPASINILDMAKVSPRFHA
                     RYDAKHRSYVYHISRRRTAFGKDYCWWVKEPLNVEAMAAVTHHFTGMKDYKSFGAPEK
                     EGESTLVKIDHLKIFEVDDSILIHIVGSHFLWKQVRRMVGTLVQVGLGKLSEADIDSF
                     FSEYSTLPAKFTAPPSGLYLEHVYYAGESINQEPTWLINIGKY"
     gene            3581..>4202
                     /locus_tag="PK049_08930"
     CDS             3581..>4202
                     /locus_tag="PK049_08930"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013264992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOK61723.1"
                     /translation="MDFKLLKTDSQTSARAGIITTDHGKIETPIFMPVGTVGTVKGVS
                     HRELENDINAQIILGNTYHLYLRPGLDVLKQAGGLHEFSSWNRPILTDSGGYQVFSLA
                     DMRKLTAQGASFRSHIDGSPHLFTPENVMDIQRIIGADIVMAFDECPPGDADYEYARK
                     SLDLTEKWLERCVHRFDTTEPLYGHSQTLFPIVQGCVYPDLRKRAAE"
     assembly_gap    4203..4302
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4303..4866
                     /locus_tag="PK049_08935"
     CDS             <4303..4866
                     /locus_tag="PK049_08935"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOK61724.1"
                     /translation="FPIVQGCVYPDLRKRAAENVQKYNRDGYAIGGLSVGEPAEVMYQ
                     MVEVVNGILPKSKPRYLMGVGTPENILESIALGIDMFDCVMPTRNGRNGMLFTSEGII
                     NIRNKKWQADFTPIDPFGPTFVDSRYTKAYLRHLFIAGEMLGPQIASIHNLGFYLWLV
                     GEARKHIVEGDFLQWKNAMVKKVSKRL"
     gene            4876..5952
                     /locus_tag="PK049_08940"
     CDS             4876..5952
                     /locus_tag="PK049_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOK61725.1"
                     /translation="MKLLDWYIIRKFIGTYFYAIILIVGIAVIFDLAEKIDDFLEKDA
                     PMYDIIFRYYLNFIPYFANLFSSLFVFIAVIFFTSKMAYNTEIIAILSSGVSFKRMLY
                     PYFISATIIAIFSFVLNSFIIPPANAVRLDFENKYIKRPYVNTDRNIHRQVRPGMFVF
                     MRSFNTYNNTAYNLSIEKFDGNKLVSKLIADNAQWDSTKEKWTINRYYIRNYTDSGEE
                     IIKGDKIDSTFYLTPSDFNQRLKIIETMNLFQLNDFIAEQKLQGADTIEYLLIEKHGR
                     FAYPFSVFILTLIGVSLSSRKVRGGIGLHIGLGLALSFSYILFMRFSTMFAVGGHLPP
                     AISVWVPNFLFAIIAVGLYRMAPK"
     gene            6128..7672
                     /locus_tag="PK049_08945"
     CDS             6128..7672
                     /locus_tag="PK049_08945"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOK61726.1"
                     /translation="MPLKNKIEELKKRKEEVLQGGGEQAVAKQVAMGKLTARERIIAL
                     LDEDSFNEYDMFVEHDARDFGMDGKVLHGDGVIIGTGTIFGAPVAVYAQDFTVAGGSL
                     GSMHARKITKIMDYALRMRIPLIGINDSGGARIQEGVNSLAGYGEIFWRNTLSSGIIP
                     QISVILGPCAGGAVYSPALTDFVFVVDKISKMFITGPEVIKSVLGEEISQEDLGGAKV
                     HAQITGNAHFYAESEYECFEQIKKLITYIPWNNTRKARRFEPKEPKVNFNIEDIVPGD
                     PKEPYDVRDVIRAIADDSDFFEIQEKWAANIVIGFGRLNGETVGFVANQPLVMAGVLD
                     VDSSDKAARFVRYCDAFNIPLVTLVDLPGYLPGIDQEHAGVIRHGAKLLYAYSEATVP
                     KMTVILRKAYGGGYIAMSSRHLRADFVFAWPSAEIAVMGPEGAANIIFRKEISEAPDP
                     EAMRRQKVEEYKEKFANPYVAAAKGYIDTVIEPAETRKLLLHALEVSSTKVADRPFKK
                     HGIPPF"
     gene            7714..7947
                     /locus_tag="PK049_08950"
     CDS             7714..7947
                     /locus_tag="PK049_08950"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HOK61727.1"
                     /translation="MIHPKLDDKNVTAFIPGTIIDVKVKKGQEVKQGDVLVILDAMKM
                     HNRLIAPSAGKVKEVKVKPGDRVSKGALLVSIE"
     gene            8372..11455
                     /locus_tag="PK049_08955"
     CDS             8372..11455
                     /locus_tag="PK049_08955"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOK61728.1"
                     /translation="MTFNEDSRVKIPAIVHLTRLGFTYVPKSGITNLHADTNIFKDLF
                     KQGLSKINGTEYSDSEIETFLSEINIQLDNSDLGKAFYKSLQGDFACKLIDLENFDNN
                     LFHVVTELTYKNDEEEFRPDIILLINGMPLAFIEVKKPNNREGILAERNRINTRFKNP
                     KFKKFMNITQLLVFSNNQEYDEENITPIQGAFYATPDPEQVNFNCFREEDNSLIHNVP
                     QEDSEIEKQILLDNNIVSILGTKEYETAKGPNSPTNRIITSLFSRQRLKILLRYGFAY
                     VNTVNNGITKIEKHIMRYPQLFATLAIEKKLIAGVKKGIIWHTQGSGKTALAYYNVNY
                     LKDYYQKQNVIAKFYFVVDRLDLATQAKNEFEARGLKVDMVSSKEDFIKNIKTAGATS
                     GNTGAQTITVVNIHKFSAESISKVSDYDLNIQRIYFLDEVHRSYNPQGSFLSNLIASD
                     RNAVLIGLTGTPIISKEYKSKDIFGDYIHKYFYNQSIADGYTLKLIREAIETKFKAEI
                     NQVYDEIVKQGEFTKSQIFAHPKFVEPLVDYIITDFKKSRVLHNDQSIGGMIVCDTSD
                     QAKMIFAEIQKYNERLGAQQKVYHEPQTDYYIAAESGVGYLTPELAPISIALILHDID
                     TKEIRKEHQTDFKQGKIDLLVVYNMLLTGFDAKRLKKLYLTRVVKEHNLLQTLTRVNR
                     PYKNFKYGYVVDFADIRKEFDKTNKEYFKELQEELGDETKNYSNLFKSAEEIDKEIQE
                     IKEKLFLYDFSNIEEFQQIVSQITDKKEIIELKKCLENLKSLYNIIKIMGYTELLDKF
                     SFDRVNKLYNEVANRIDILNLKENLENAADNTNLLNLALENMQFTFRKIATHELQIAD
                     KFRSELERTRKELERNFDKKDPKFISLFEELKRLFKKKNIEELTSEEMDEAINDLKKI
                     YEQANALNNKDAMLAAKYENDIKFARIHKRIKEQNMNVLNSDLALHETLIFIKHETDK
                     TVVNNQAILNNPDYFAEITKRTILETLENKGIRDLNVVRFFNNAVVNEYFYQRAI"
     gene            11458..11919
                     /gene="tnpA"
                     /locus_tag="PK049_08960"
     CDS             11458..11919
                     /gene="tnpA"
                     /locus_tag="PK049_08960"
                     /inference="COORDINATES: protein motif:HMM:NF033573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HOK61729.1"
                     /translation="MPGTFSQIYIQIIFAVKGRGSLIYSDWEERLYQYITGIVRGKEQ
                     KMIAINGMPDHIHIFIGMKPSCCLSDLVREIKKASNEFINENKLSNFRFSWQEGYGAF
                     SYSHSQIDNVVKYIMNQKQHHRKVSFREEYVDFLKKFEIEHDDKFLFEWVK"
     assembly_gap    12157..12256
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12482..14125
                     /locus_tag="PK049_08965"
     CDS             12482..14125
                     /locus_tag="PK049_08965"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HOK61730.1"
                     /translation="MTKHLEYTQKTKDLIDGLKTVCSNYGLANSGNEYKIITEVFLYK
                     FLNDKFLHEARKANKDLKNSTNIETDIQKMSNSDYEWMLEKIGERSAKLQKHHFISYL
                     FNKQNEPNFHELFDKTLLEIADLNIKLYSVSTIDKERVRLFDRISPYIIESNQKTPFC
                     KAIINKLVAFSFEPIFEEKYDFFSTIFEYLIKDYNKDSGQYAEYYTPPAVAKIMAKIL
                     VPEPTRNVTVYDPAAGSGSLLLALAHQIGENKCTIYSQDISQKSSEFLRLNLILNNLV
                     HSLHNVIKGNTLTNPYHVNDAKNDLKKFDYIVSNPPFKMDFSDDRETLASDQHRKRFF
                     AGVPSVPKKKKESMAIYQLFIQHILYSLNDKGKAAIVVPTGFCTEKAAIALAIRKKIV
                     DSNWLHAVVQMPPNIFATTGTNVSVIFIDKSKTRDTAILVDASKLGTKVKDGKNQKTL
                     LSNEDEKRIIDTIKGYRQLDDFSVIVNNEQMKQKAYSFGAGQYFETKIEYVELSPEEF
                     EERINEFKNKISFYFNNSRELEIQILKNFNKLDYKKNNK"
     gene            14122..15336
                     /locus_tag="PK049_08970"
     CDS             14122..15336
                     /locus_tag="PK049_08970"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOK61731.1"
                     /translation="MKKELLGDRVELIYGFSVRTDNEPKGKYPVYGSNGIIGFIDSFK
                     VKGPGIIIGRKGTVGAVTYSSENFTPTDTTYYVALKDNTKDDLKFWYYYLQLIGLDKL
                     NSHSTVPGLSRDLAYLKEIRVPEKAEQKLIADFLTVIDDKIALNKRINTELENLAKTI
                     YNYWFVQFDFPNNEGKPYKSSGGEMEWNEELKMEIPKGWKTKSLYDIAIYTNGLAMQK
                     HRPNGDEKLPVIKIKEMNEGYSAATEYARPDIEDAYLIREGDVLFSWSASLNVMIWAD
                     KVGALNQHIFKVTSEFYPRYYFYHQISNYLNHFKLMAENRKTTMGHITIDHLKESRIV
                     VPDDDSLILKCGERIEPILDLIVKNRMTNIDLMEVRDFLLPMLMNGQVKVKSEAKEQL
                     SIAAEPQVKYGK"
BASE COUNT         4887 a         3188 c         3022 g         4039 t
ORIGIN      
        1 ccaacaaact aatcctaaaa aaatcaccat aaaaaaaggg tcgcaattgc ttgcggccct
       61 tttgtcttct attctttaac tctctactct gttctttcgt tattcataaa cccctgcatt
      121 tccaaaaccc tcagtaaatt ctttgagaat gccacattgt ccttcagggt gtaacgctta
      181 tcggtaaaaa tttgagccag tgtttccttt tggttctcct ccagcaaacg cttggttagc
      241 ggtaacgact ccttatgctg ataaatttga tcgatatcac cctcagagta atcgtggtaa
      301 acatcgtagt gcacaacacc tgcaagcggc aacctatcgg ttaagtcggg gtgctcatcg
      361 ggataaccca ttaccactgt tgttacaggc acaacaccct tgggtaattt cagcacctca
      421 ataatcttat cggcagtata ggttgtagtt ccaaggtaac atattccaag acccttctcc
      481 tcggcggcaa tgcatacgtt ttgggctacc aaaagggcat caattgcagc ggtaaagaac
      541 gacaaaaagt tatcgtaccc cgggttggcc ttacgctgta agcaccactt gttaaagcgg
      601 ttaaaatccg cacaaaaggt gagcaccact ggtgcctgca ctaccatctg ctggttaaaa
      661 tgcaatggta aaagcttacg cttcacctcc tcatcgcgtg taacaattat gctgtacacc
      721 tgcatattac cagtggtaga tgcacgagtt cctgcggtta ggatttcctg aagtagcgaa
      781 tcgtcaatgg ggtcgctctt gtactttcga attgaacgat gcgatagcaa tgctttcatc
      841 tcattcttga tttatcaact tacaaataaa ataaaatagc actacggaag tgctattttg
      901 atgtatgtta ttgaacaact ttttaaaagt aatggttatc ctaactcctt ctcaatgagg
      961 taatggtttc tgtcggcaga gctgcgcgac accaactcac caataaaacc tgccgtaaag
     1021 agttggacac caattatggc aaccacaaga gctatgtaaa aaagtggttg atcggtaaca
     1081 ggcctgtact ttaaatgatg aaactgtgcc caaagcttac tacctaccaa ccatacggca
     1141 atagcaccac cggtaataaa cataagggtt ccaagggttc caaagaagtg cataggcttt
     1201 ttaccaaagc gcgacacaaa catcaccgat agaaggtcaa gaaatccatt gataaacctt
     1261 gtccagccaa attttgtggt gccgtacttg cgctccctgt gctgcacaac cttttcgcca
     1321 attcgcttaa atccggcctg acgggcaatt acaggaatat acctatgcat ttcgccataa
     1381 acctcaatgc tcttaaccac attgtggcgg taggccttaa ggccacagtt catatcgtgc
     1441 agctttatac cgcttaccat acgcacggtg gcattgtaaa gtttactggg gatccgcttg
     1501 gtaagcaacg gatcgaaccg cttcttcttc caacccgaaa caaggtcgta gccttgttcc
     1561 ttgaccatac ggtaaagctc aggaatttca tcggggctat cctgtaggtc agcgtccatg
     1621 gtaaacacca catcgccttg agcggctgta aagccaacct gcagggcagc cgatttaccg
     1681 tagttcctca gaaacttaat ccccctcagg gatggaaact tgagcctcag gctctcaatt
     1741 accgcccagc tgttatcgtt actaccatcg tcaaccataa ttacctcgta ggtaaaatgg
     1801 ttctgggtca taacctgggc aatccattcc attaactcgg ggagcgactc ctcctcgttg
     1861 tacagaggaa ctacgataga aatatccata ttgatgtttg gttactattc aataacttca
     1921 ccgccaccag caaaaggcga tttttccttc ttcagaaaga tagaagtgat cagtgagaat
     1981 atgaatccca tcagaaccat tccgagtaac gatgaaatag tcatgattag cggagtcata
     2041 aactttttct gcatctctac agccatttca atttgctcat cgggcatacc cctctttaca
     2101 agttcctcct cagcaatggc taacatctta cttacaaagt cgggatcaat aagggtaatt
     2161 tgaatataag taaatacagc cacaatcact cccgcaaaaa gtgtaattaa aacaccaaag
     2221 cccaaggcat taccataggt aattgcaccc tctaacacct catcgcgata cgttttagta
     2281 ccccatacga taataccaat tataatggca tagtttatta tgctaaccca aaacggtggt
     2341 tttacaatac ctgccaagta agtaatcagg cttagaatta tgagtgcaac acccattatt
     2401 aaaccgtaat tcattgaatg cttaagcata gggtttcctt gtttttccat ttttgtattc
     2461 ggtttagttt cattgcaaat atatgaattt tgctaaaaaa agctagtagg ttcaacaggt
     2521 attgtaaagt ttagctatct ttgcagcggg taagtcttat acgaccagct cctgctgaac
     2581 ccccccagga ccggaaggta gcaagggtaa gcggttgtag cggtgcgata taagtagctt
     2641 acccactttt tttatacctc attttaagcg taactttgta aaaatttcaa agaaaaatcg
     2701 caaatgcctc gatttaagtt aaccatagag tacgacggtt caaggtaccg cgggtggcaa
     2761 ttccagcgcg aagccgccac agtaatgggc aagctaatgg atgccatcaa ggaggtttac
     2821 aaggtaagca gctatgagct ttacggagca ggacgtaccg atgctggcgt gcacgctctg
     2881 gggcaggttg cccacctcga tatcgactcg gcaatgccac cctacgttgc catggaaagg
     2941 atgaatgagg ttcttcctgc atcaatcaat atccttgata tggcaaaggt ttcacctcgc
     3001 ttccacgcac gctacgatgc caagcaccgc agctacgtgt accacatttc gcgcaggcga
     3061 acagcctttg gcaaggacta ctgctggtgg gttaaggaac cgcttaacgt tgaggctatg
     3121 gcagccgtta cccatcactt taccgggatg aaggactaca aatcgtttgg cgcacctgaa
     3181 aaggaaggag aatcgaccct tgtaaaaatt gaccacctaa aaatatttga ggttgacgat
     3241 tcaattctca tacatatcgt ggggtcacac ttcctttgga agcaggtacg ccgaatggtg
     3301 ggcacactgg ttcaggttgg actgggcaaa ttatcggagg ccgatatcga ctcgttcttc
     3361 agtgagtact caacccttcc ggcaaagttt actgctcccc catcggggtt gtacctggaa
     3421 cacgtttact atgccggcga aagcataaac caggagccaa cctggttaat aaacattggc
     3481 aagtattaat ttaaaacctg agtatttacc gttaacaaat caaaataaac ccttaacctg
     3541 ttagtattta ggttatattt gtgcggattt ttacaagcag atggatttca agttactcaa
     3601 aaccgattca caaacctcgg cacgtgcagg tatcataaca accgatcacg gtaaaattga
     3661 aaccccaata ttcatgcctg ttggcacggt gggcaccgtt aagggtgttt cgcacaggga
     3721 gttggagaac gacattaacg cccaaatcat cctgggcaat acataccatc tttatctacg
     3781 tccgggactc gatgtactaa agcaagccgg cgggttacat gaattctcga gctggaatag
     3841 acccattctt accgatagtg gcggatatca ggtattttcc cttgccgaca tgcgaaagct
     3901 aacagcgcag ggcgcttcgt tccgctcgca tatcgatggc tcgccccacc tgttcacccc
     3961 agagaatgtg atggacattc agcgcattat tggggccgat attgtaatgg cattcgacga
     4021 atgcccaccg ggcgatgccg attacgagta tgcccgcaag tcgctcgatc ttaccgagaa
     4081 atggcttgaa cgctgcgtgc atcggttcga taccaccgag ccgctttacg gccatagcca
     4141 aacgctattc ccaatcgtcc agggctgtgt ttaccccgat ctgcgtaaac gcgctgccga
     4201 aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnttcccaat cgtccagggc
     4321 tgtgtttacc ccgatctgcg taaacgcgct gccgaaaatg tacagaagta caaccgcgat
     4381 ggctacgcca ttggtggact atcggttggc gaacccgccg aggtaatgta ccaaatggtt
     4441 gaagtggtaa acggcatact gcccaagagt aaaccccgct acctgatggg cgtaggtacg
     4501 cctgagaaca tcctggaatc cattgcccta ggcattgaca tgttcgattg cgtaatgccc
     4561 acccgtaacg gacgtaacgg catgcttttc acctctgagg gaattataaa catccgaaac
     4621 aaaaagtggc aggccgattt cacacctatc gatccttttg gcccaacttt tgttgatagt
     4681 agatacacaa aggcttacct ccgccacctt tttattgcgg gcgaaatgct aggcccacaa
     4741 atcgccagca ttcacaacct agggttttac ttatggttag tgggggaagc acgaaaacat
     4801 attgttgagg gcgatttcct gcaatggaaa aacgctatgg ttaaaaaagt gtcgaaacgt
     4861 ctataaaaca cctctatgaa gttactggac tggtatataa tccgaaagtt tattggcaca
     4921 tacttctatg ccataatact aatagttggc attgcagtaa tatttgacct tgccgagaaa
     4981 attgacgact ttctggagaa ggatgcccca atgtacgata tcattttcag gtattacctc
     5041 aactttatac cctactttgc caacctgttc agctcccttt tcgtattcat tgccgttatt
     5101 ttcttcactt caaaaatggc ctataatact gagattattg ccatcctgag cagcggtgtt
     5161 agctttaagc gtatgttata cccctacttt atctccgcaa caataattgc cattttctca
     5221 tttgtgctca atagctttat aattccaccc gccaatgctg taaggttaga ctttgagaac
     5281 aagtacatca aacgacccta tgttaatacc gacaggaaca tacaccgaca ggtgcgtccg
     5341 ggaatgtttg ttttcatgcg ctcattcaac acttacaaca acaccgccta caacctttcc
     5401 attgaaaaat ttgatgggaa taagctggta tcgaaactca tagccgataa tgcacagtgg
     5461 gattccacca aggagaaatg gactataaac cgctactaca tccgtaatta tactgacagt
     5521 ggcgaagaaa ttatcaaagg cgataaaatt gatagcacat tctacctcac cccatccgac
     5581 ttcaaccaga ggttgaagat cattgaaacc atgaacctat ttcagctaaa cgattttata
     5641 gcggaacaaa agctccaggg cgccgatacc attgaatacc tgctaattga aaagcacggg
     5701 cgatttgcct accccttttc ggtgttcatt cttaccctga ttggcgtatc gctttcatcg
     5761 cgaaaggtac gcggtggcat aggcctgcat attggactgg ggctggcgct gagcttctcg
     5821 tatatcctat tcatgcgatt ttcaaccatg tttgccgttg gcggacatct accaccagcc
     5881 atctcggtat gggttcccaa cttcctgttt gcaatcatag cagtgggtct ttacaggatg
     5941 gcacccaagt aaacattaaa caggtgttta ctggtgtagg attttgatat ttaggctaat
     6001 acattatagc gggtcgaaaa aaacggcccg tttcttttat atttgcaccc aatttcgtaa
     6061 ccttgcacaa aactttacca cgatgcatgg taatggtggc aaaaattact aaccaacaaa
     6121 aacagctatg cctttaaaaa acaaaattga agagctaaaa aaacgcaaag aggaggttct
     6181 gcagggtgga ggcgaacaag ccgttgccaa gcaggttgcc atgggtaagc tcactgcccg
     6241 tgagcgtatt attgccctcc tcgacgagga ctcgttcaac gagtacgaca tgttcgtgga
     6301 gcacgatgcc cgcgactttg ggatggatgg taaggttcta catggcgatg gtgttatcat
     6361 tggtaccgga accatttttg gtgctcctgt ggcggtttac gctcaggact ttaccgttgc
     6421 cggtggttca ctgggttcaa tgcacgcccg taagattacc aaaatcatgg attacgcact
     6481 ccgcatgcgt atccccctaa tcggtatcaa cgactcaggt ggtgcccgta ttcaggaagg
     6541 ggttaactcg cttgccggtt acggcgaaat attctggcga aataccctct caagcggtat
     6601 tattccacaa atatcagtaa tccttggccc ttgcgccggt ggtgcagttt actcccctgc
     6661 cctaaccgat tttgtgtttg tggtcgataa aatctcaaag atgttcatca ctggtcccga
     6721 ggttatcaaa tcggttctgg gcgaggaaat ctcccaggaa gaccttggtg gcgcaaaggt
     6781 tcatgcccaa attactggca acgcacattt ctacgctgag agcgagtacg agtgctttga
     6841 gcagattaaa aagcttatca cctacattcc ctggaacaat acccgcaagg ctcgcaggtt
     6901 tgagcccaaa gaaccaaagg taaacttcaa tatcgaggat attgtaccag gcgatccaaa
     6961 ggagccctac gatgtacgtg atgttatccg tgccattgcc gacgattccg atttcttcga
     7021 aatccaggag aagtgggctg ccaatattgt tattggcttt ggccgactga atggcgaaac
     7081 cgttggcttt gtggctaacc agccactggt tatggccggt gtactcgacg ttgacagctc
     7141 cgacaaggct gcacgctttg ttcgttactg cgacgcattc aacattccgc tggttaccct
     7201 ggttgaccta cccggttacc tacccggtat cgatcaggaa catgcaggcg ttatacgcca
     7261 cggagccaag ctgctttacg cttacagcga agcaaccgta cccaaaatga cagtaattct
     7321 gcgtaaagcc tatggtggag gctatatagc catgagttcc cgccacctac gtgccgattt
     7381 tgtatttgca tggccaagcg ctgagattgc cgttatgggc cccgagggtg ccgccaacat
     7441 catcttccgc aaggagattt ccgaagcacc cgatccggaa gctatgcgtc gccaaaaagt
     7501 tgaagagtac aaggaaaagt ttgctaaccc atacgtagcc gcagccaaag gctatatcga
     7561 taccgtgatt gaaccggccg aaacccgtaa gctgctgctg cacgcccttg aggtttccag
     7621 cactaaggtt gccgaccgcc ccttcaagaa acatggtatt cctccatttt agtcaaccca
     7681 acaatccttt tgtaaactaa aaaacttgta gctatgatac atccaaaact cgacgataag
     7741 aacgttacgg cctttatacc cggaaccatt attgatgtaa aggttaaaaa gggtcaggag
     7801 gttaagcaag gcgatgtgct ggtgatactt gacgccatga agatgcacaa ccgcctgata
     7861 gccccctcgg ccgggaaagt gaaagaggtt aaggtaaaac ccggcgacag ggttagcaaa
     7921 ggcgccctgc tggtaagcat tgagtaaaaa accgattcta agcataaaaa acctccgcaa
     7981 tgcggaggtt ttttgttacg gaaagggcgt tattcaaaaa ccgttatgca ttttacgcaa
     8041 ggcgatataa actataaaaa caaagtggct ggttaatgaa tttaattttt tactacttta
     8101 gttacatgaa acagggctag ttccaatcat agctatacac ataatcatag gtgtattagt
     8161 ataattcttt aggatatgaa ctaataaact gtaaacaaaa aaatagaagt gaacaaataa
     8221 cgaaacgaca atgataaaaa ttttcccaca catatgacgc atttgatttc tacaagcact
     8281 aaagcaagcc tatagctaaa ccaaaagaac cttttttact aattcactca taaaaaccgt
     8341 attttgtact attaaattat taagagataa aatgaccttt aatgaagatt caagagtaaa
     8401 aattcctgct atcgtgcatt tgacacgatt gggatttact tatgttccaa agtctggcat
     8461 tacaaatctt catgcagaca caaatatttt caaagacctg ttcaaacaag gtctttcaaa
     8521 gattaacgga actgaatatt cggacagtga aattgaaact ttcctttctg aaatcaatat
     8581 ccagctggac aattcagacc ttggaaaagc attttataaa agcttgcaag gtgactttgc
     8641 ctgcaaattg attgacttag aaaatttcga caacaacctt tttcatgttg taaccgaact
     8701 gacttacaaa aatgacgaag aagaattccg acctgacatc attttattga ttaacggaat
     8761 gcctttggct ttcattgaag taaagaaacc taataaccgt gaaggaattt tagcggaacg
     8821 gaacagaatc aacacacgat tcaaaaatcc gaaattcaaa aagttcatga acattactca
     8881 gttgttggtt ttctccaaca atcaagagta tgacgaagaa aacatcaccc cgatacaggg
     8941 agcattttac gcaacacctg accctgaaca ggtaaatttc aactgtttca gagaagaaga
     9001 caattcatta attcataatg ttccgcaaga ggacagcgaa atagagaaac aaatactgct
     9061 cgacaataac attgtttcca ttcttggaac taaggagtat gaaacggcaa aaggtccaaa
     9121 ctctcctact aaccgtatca taacatcact attcagcaga caacgtttaa aaatattgct
     9181 tcgttatggc tttgcttatg tgaacaccgt taacaatgga ataacaaaga ttgaaaagca
     9241 catcatgcgg tatccgcagt tgtttgcaac tttagctatt gagaaaaaat taatagcagg
     9301 agtaaaaaaa ggaatcatat ggcatacaca aggaagcggc aaaacagctt tggcatacta
     9361 taatgtaaat tacctgaaag actattatca aaagcaaaat gtaatagcca agttctattt
     9421 tgtggtcgac cgtttggact tagccacaca agccaaaaac gaatttgaag caaggggatt
     9481 gaaagtcgat atggtcagtt ctaaagagga cttcataaaa aacattaaaa ctgcaggagc
     9541 aacctcagga aacacaggtg cacaaaccat aaccgttgta aacattcata agttttctgc
     9601 tgaatccatc tcaaaagttt ctgattacga tttgaacatt caacgaattt attttttaga
     9661 tgaagttcac cgaagttata atccacaggg ttcttttctt tcaaacctaa ttgcttccga
     9721 tcgtaatgct gttttaatag gtttaaccgg tactcctatc atttccaaag aatataaaag
     9781 caaggatatt tttggggatt atattcataa atacttttac aatcaatcca ttgcggatgg
     9841 ctatacgctg aaacttataa gggaagccat tgaaaccaaa ttcaaagctg aaatcaatca
     9901 ggtgtatgat gaaattgtga agcaaggcga gttcaccaaa agccaaattt ttgctcatcc
     9961 caaatttgtt gagccattgg ttgattacat cattactgac ttcaaaaaaa gcagagtgtt
    10021 gcacaacgac caaagtattg ggggaatgat tgtttgcgac acttctgacc aggcgaaaat
    10081 gatttttgct gaaattcaga aatacaatga acgtctggga gcccaacaga aagtatacca
    10141 tgaaccacaa actgattact acatcgcagc tgaatcggga gtgggctatc taacacctga
    10201 gttggctcca atttcaattg ctttaatact gcatgatata gacaccaaag aaatccgcaa
    10261 ggaacatcaa accgatttca aacaaggaaa aattgatttg ttggtggttt acaatatgct
    10321 acttaccggt tttgatgcca aacgactgaa aaaattgtat ctgacccgcg ttgtgaaaga
    10381 acacaatctt ttacagacac taacaagggt aaaccgacct tataagaatt ttaaatacgg
    10441 ctatgtggtt gactttgccg acattcgcaa agaatttgat aaaaccaaca aggagtattt
    10501 caaagaatta caggaagaat tgggcgatga aactaagaac tactccaacc ttttcaaatc
    10561 tgccgaagaa atagacaagg aaattcagga aatcaaagaa aagctatttc tttatgactt
    10621 ttctaatata gaagaatttc aacaaattgt aagccagata actgacaaaa aggaaattat
    10681 cgaactgaaa aagtgtttgg agaacttaaa aagcttatac aacatcatca aaataatggg
    10741 ttacactgaa ctgttagaca aattctcatt tgacagggtg aacaagttgt ataatgaagt
    10801 ggcaaaccgt attgatatcc ttaacctgaa agagaattta gaaaacgcag cggacaatac
    10861 aaatcttttg aatttagcac ttgaaaacat gcagtttaca ttcaggaaaa ttgctacgca
    10921 tgaattgcaa attgccgata aattcagaag cgaactggaa agaacaagaa aagaactaga
    10981 gagaaatttt gataaaaaag acccgaaatt tatatctctt tttgaagagc taaaaaggct
    11041 tttcaagaaa aagaacattg aagaattaac atctgaggag atggatgagg ctatcaatga
    11101 tttgaaaaag atttacgaac aagccaatgc gcttaacaat aaagatgcaa tgctggccgc
    11161 caaatatgag aacgacatca aatttgcccg cattcacaaa cgcatcaagg aacaaaacat
    11221 gaatgtactg aacagcgatt tagcattaca tgaaactttg attttcatta aacatgaaac
    11281 cgacaaaaca gtagtaaaca atcaggcaat acttaacaac ccagattatt ttgccgaaat
    11341 tactaaacgt accattctgg aaacattgga aaataaaggc atccgtgacc tgaatgtagt
    11401 tcgatttttc aacaacgctg tagtaaacga atatttctat caaagagcca tataaatatg
    11461 ccaggaacat tttctcagat atatattcaa attatttttg cagttaaagg cagaggaagc
    11521 ttaatttatt ctgattggga agaaagattg tatcaataca ttactggaat tgtaagggga
    11581 aaagaacaga aaatgattgc tattaatgga atgccagacc atattcatat ttttattggt
    11641 atgaaaccat cttgttgcct atcagattta gtgagagaga ttaaaaaggc atcaaacgag
    11701 tttataaatg aaaataaact ctccaatttc agatttagtt ggcaggaagg atacggtgca
    11761 ttttcatata gccattcgca aattgataat gttgttaaat atataatgaa tcagaaacaa
    11821 catcatcgaa aagtgagttt ccgagaagaa tatgttgatt ttttgaaaaa atttgaaatt
    11881 gaacatgatg ataaattttt atttgaatgg gttaaataat gtgataccga aggtatcaaa
    11941 gatttataga aaatatatca ttcatccatc tacgacccct gcggggtcgc accatttcgc
    12001 atatcaaatt ttctataaac ctgtaatccc ttcgggatta gatgatgatg tagccctaat
    12061 gtcgaaaaca tcaaaggtct atagcattaa cgataaccaa acatctacga cccctgcggg
    12121 gtcgcaccat ttcgcatatc aaattttcta taaaccnnnn nnnnnnnnnn nnnnnnnnnn
    12181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12241 nnnnnnnnnn nnnnnncatc tacgacccct gcggggtcgc accatttcgc atatcaaatt
    12301 ttctataaac ctgtaatccc ttcgggatta gatgacgatg tagccctaat gccgaaggca
    12361 ttaaagtcta taacttcagc gacaatcgat tacctacgac ccctgcgggg tcgcacaata
    12421 aaccctattg caacacctat caacctgcaa tctctttggg aataataaaa taaattattc
    12481 tatgactaaa caccttgaat acactcaaaa aacaaaagat ctaatagacg gccttaaaac
    12541 cgtttgcagc aattacggtt tggctaattc cgggaatgaa tacaaaatca ttacggaagt
    12601 tttcctgtat aaatttctga acgacaaatt cctgcacgaa gcacggaaag ccaataaaga
    12661 tttaaagaac agcaccaaca ttgaaaccga catccagaaa atgagtaaca gcgactatga
    12721 atggatgctg gaaaaaatcg gtgaacgtag cgccaaactg caaaagcatc atttcatcag
    12781 ctatttgttc aataaacaga acgagcccaa ttttcacgaa ttgttcgaca aaacattact
    12841 ggaaattgcc gatttgaaca taaaacttta ttctgtttca accattgata aagaaagggt
    12901 tcgtttgttt gatagaattt ccccttacat cattgaaagc aatcaaaaaa cacctttctg
    12961 caaagccatt atcaacaaac tggtagcgtt tagctttgag ccaatttttg aagaaaaata
    13021 tgattttttc tctaccattt ttgaatacct gattaaggac tacaacaaag acagcggaca
    13081 gtatgccgag tattacacac caccggccgt ggcaaaaatc atggcgaaga ttttagtgcc
    13141 tgaacccacc agaaatgtta ctgtttacga ccccgcagca ggttcaggaa gtttattact
    13201 ggcgctggct caccaaatcg gagaaaacaa atgcaccatt tattcgcagg acatttcgca
    13261 aaaatcatca gagtttcttc gcttgaattt gatattgaac aatctggtgc attcgctgca
    13321 taatgtaata aaaggtaaca cacttaccaa tccataccat gtaaacgatg ccaaaaacga
    13381 cctgaaaaag tttgactaca tcgtaagcaa tccaccattc aaaatggatt tcagcgatga
    13441 ccgtgaaacc cttgccagcg accaacatag aaaacgcttt tttgcaggcg ttcccagcgt
    13501 tccaaaaaag aaaaaagagt ccatggctat ctatcagttg ttcatacaac atatcttgta
    13561 ttcgttgaac gacaaaggga aagccgccat tgtggtacct accggttttt gtaccgaaaa
    13621 agccgccatt gcgttagcta tcagaaaaaa aattgtggat agtaactggc tgcacgctgt
    13681 ggtgcaaatg ccgcccaaca tatttgctac taccggaaca aacgtttcgg tcatttttat
    13741 agataaaagt aaaacgagag atacggctat actggttgat gcaagtaagt taggaacaaa
    13801 agtaaaagac ggtaagaacc agaaaacctt attgagcaat gaagatgaaa aacggataat
    13861 agacaccatt aagggataca ggcaattgga tgatttttcg gtgattgtta ataatgaaca
    13921 gatgaaacaa aaagcatatt cctttggtgc aggacaatat tttgaaacta agattgaata
    13981 tgttgaacta agtccagaag aatttgaaga gagaataaat gagtttaaaa acaaaatctc
    14041 tttttatttt aataattcaa gagaattaga aatacaaatt ttgaaaaact ttaataaact
    14101 tgattataaa aaaaataaca aatgaaaaaa gagttgcttg gcgatagagt agaattaatt
    14161 tacggatttt cagttagaac tgacaatgaa ccaaaaggta aatacccagt atatggttct
    14221 aatggaatca ttggttttat tgatagtttc aaggtaaagg gacctggtat tattattgga
    14281 agaaaaggaa cagtaggtgc tgttacctac tcatcagaaa actttacacc aactgataca
    14341 acttattatg ttgctttaaa agacaatact aaagacgacc ttaaattttg gtattattat
    14401 cttcaactaa ttggattgga taaactaaat tcccatagta cagtgccagg tttaagcaga
    14461 gatttagctt atctaaaaga gattagagtt ccagaaaaag ctgagcaaaa acttatagca
    14521 gattttctca cagttattga tgataagata gccttgaata aaagaatcaa caccgagctt
    14581 gaaaaccttg ccaagaccat ttacaactat tggtttgttc agtttgattt tcccaacaat
    14641 gaaggcaaac cctataaatc cagtggcggt gaaatggaat ggaatgagga attgaaaatg
    14701 gagataccga agggatggaa aactaaaagc ttatatgaca ttgctattta tacgaatggt
    14761 ttagcaatgc aaaagcatcg tccaaacggt gatgaaaaat tgcctgtcat taaaatcaag
    14821 gaaatgaatg aaggttattc agctgcaact gaatacgcca gaccggatat tgaagatgca
    14881 tacttaattc gagaaggaga tgtacttttc tcttggagtg cgagccttaa tgtcatgatt
    14941 tgggctgata aagttggagc actaaatcaa catattttca aagtaacatc ggagttttac
    15001 cccagatact atttctatca tcagatttca aactatttga atcatttcaa attaatggct
    15061 gagaatagaa aaaccacaat gggacatatc acaatagacc atctcaaaga aagtagaatt
    15121 gtagttcctg atgatgattc gttaattctt aaatgtggag aaagaattga gccgattctt
    15181 gatttgattg taaaaaaccg aatgaccaat attgatttaa tggaagttcg tgactttctt
    15241 ttgcccatgc taatgaatgg ccaagtgaaa gtaaaaagcg aagcaaaaga acaattatca
    15301 atagcagccg agccgcaggt aaaatatggg aaatga
//
LOCUS       DAOSAL010000065        15237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_2668_length_15237_cov_47.533395, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000065 DAOSAL010000000
VERSION     DAOSAL010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 15237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15237
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2668_length_15237_cov_47.533395"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            836..2884
                     /locus_tag="PK049_08975"
     CDS             836..2884
                     /locus_tag="PK049_08975"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HOK61732.1"
                     /translation="MKQFLSVPFACLLLVVAVYISSSYAYPQEGARTLAGKHSQSDSL
                     RGRNVPFDKGSARGVGRSATDTLKPNERNIYGYLGIQDPKSKSFAWYFDRETYDLVQT
                     QAFDSSLFLVHLILPGQKSLSPLTYLGNMGSPVQSDHFFERPVSYPFLFSKGYSSYEQ
                     PVLDRKQYNVRRPHTLLEYSTAGKRRNAEQNLRVFHTQNVNRYLNFGLQYDYFSTKGI
                     YERQLTRNNDFTAFASYYRHRVSAQGTFAYTYIRNQENGGLEDDRFIQDTVMEPNLVP
                     FRLKDASVEYRKRSFSGVVGYDIITKRVNDSLENLKSILTAKLILDANRYTRVYADTE
                     TDSSYYGNFYISTTSTHDSVYLATYQSTLLVELSQIAKYPGIPGLRAWVSGLMGSYYN
                     FTPDNFIYTYSNTKLNTSHFGLGVYSQSPYLSYSGAARFYMSGYRAADKELLGRLSIS
                     PWKSKELPYVAGELSITDREPDIFINRYFSNHYKWDNTFEKESRFRLSARLGADRFGF
                     EAGYSIEHILNYVYFDTLSLPAQEPKVTVTSAYIQKKFRLGWFNAVCKVIWQASTNAD
                     VLSLPTLAGFGALYVQYPVVKDVLTMQLGVSGFYRTEFYADAYNPALGVYHNQRVRKI
                     GNYPFVDVFLNARWKRANLFIKLDHANQGLIDNQYFTTLHYPYNPRMVKFGVSWMFYD
                     "
     gene            2964..4367
                     /locus_tag="PK049_08980"
     CDS             2964..4367
                     /locus_tag="PK049_08980"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOK61733.1"
                     /translation="MVRKVIYLRNGLIFLAMLIFFSVSSCEKKGVKDEEVQRDLADIL
                     ADGKLVAVTDYNATSYFIYKGEPMGYQYDMLRDLATYLGVKLEIVTENDIDKSFEMLR
                     KGKVDIIASSLAITAQRKEEVDFTLPHGETSQVLVQRIPAENGTPAIQKPLELSGRLV
                     YVQKSSAGAQRLKNLSDEIGGGITVVELPNYDADKLIELVATGEIDYAVCDQSVALVK
                     SNYYKNINIDIQIGFPQKTAWAVRKTSVELRKKVDTWLEGYLKTAHYRNIELKYFAQE
                     RFNRRASSDYFFIYTGKISPWDEYFKKYSQGIDWDWRLLASLVYQESRFRHDVTSHRG
                     ASGLMQMMPQTAEYFGVDSTVGPAKQIEAGVRYIKWLEDRFAAYDIPKDEMVKFVLAS
                     YNAGIGHIIDARNLAQKHGRNPNVWDNNVEYFIRNKSQFVNDPVVRHGKLKGVETTNY
                     VKEIMERYQHYKNIIGE"
     gene            4431..5966
                     /gene="gltX"
                     /locus_tag="PK049_08985"
     CDS             4431..5966
                     /gene="gltX"
                     /locus_tag="PK049_08985"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOK61734.1"
                     /translation="MSNRRVRVRFAPSPTGPLHIGGVRTALFNYFFARQHGGDFILRI
                     EDTDSQRFVPGAEEYINEALNWCGIKIDEGVREGGPHAPYRQSERKHIYKQYADRLVE
                     SGHAYYAFDTPEVLDKMRAEAEAQGGAFTYNYAIRNGMENSLALPADEVKRRIERGDQ
                     WVIRFKMPENEEVVMHDLIRGEVRVNTSTLDDKVLFKSSDMLPTYHLANVTDDYLMEI
                     SHVIRGEEWLPSLPLHVLLYRALGWENVMPKFAHLPLLLKPSGNGKLSKRDGDKMGFP
                     VFPLLWKSADGEISRGYREDGYFPEAFVNLLALLGWNPGTDQELFSMDELIKLFSIER
                     VNKSGARFDPEKAKWFNHQYLIRKSDAEVAELFQPVLEQYNIDVPFDKLTRIVGLVKE
                     RVNFVHELWGQTSYFFERHLEYDQQAVAKHWKPETPDMITDVSETLKALDTWNSHEIE
                     TALKDLINAKGYGMGKVMNAVRLCLVGESKGPGIADICEILGRDETLTRLNQAILILN
                     GKK"
     gene            6588..7358
                     /locus_tag="PK049_08990"
     CDS             6588..7358
                     /locus_tag="PK049_08990"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HOK61735.1"
                     /translation="MERNKLNYIETRRMVEDLSDSLTELVVESLLGAGQTVDTEKPYS
                     KLYEALRYFNPDTSQCQRQLEIVYQGFQGVLDTLSHDANFIYLNDPAAQSLEEVLLAY
                     PGFFAIAHYRLANLLHRVGVNILPRMITEYAHSKTGIDIHPAATIGQEFCIDHGTGVV
                     IGETTIIGQRVKLYQGVTLGALSVSKSLSATKRHPTIEDDVIIYAGSTILGGETVIGH
                     DSVIGGNVWLIKSVPPFSMVYHESKVIVQQGKFNHKMK"
     gene            7362..8273
                     /gene="cysK"
                     /locus_tag="PK049_08995"
     CDS             7362..8273
                     /gene="cysK"
                     /locus_tag="PK049_08995"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOK61736.1"
                     /translation="MKYLSIDKSIGATPHIRLSRLFPNAEVWLKDERRNPSGSIKDRA
                     AMAMVEDAERKGLLKPGSVIVEPTSGNTGIGLAMVAAVKGYRLILTMPESMSVERRVI
                     LKAYGAEIILTPAAQGMQGAIDKAKEFMAQLPSAWMPMQFANPANPNTHSLTTAKEIL
                     ADFPDGLDMVVAGVGTAGHITGVGRTLKEAFPNIQVIAVEPFDSPVLSGGQRAPHPIQ
                     GIGAGFVPQNLDRGVIDEIITVKADEAIECMRLLARTEGILAGISTGANIAAVKKLLS
                     VKQSNLRILTFAYDTGERYLSVKGIWD"
     gene            complement(8276..10855)
                     /gene="mutS"
                     /locus_tag="PK049_09000"
     CDS             complement(8276..10855)
                     /gene="mutS"
                     /locus_tag="PK049_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOK61737.1"
                     /translation="MKQYLSIKRNHPDAILLFRVGDFYETFGEDAIKTSEILGITLTR
                     RANGAASFVELAGFPHHALDTYLPKLVRAGQKVAICEQLEDPKKAKTIVKRGITELVT
                     PGVSYNDNVLERKENNFLACVYLDKKAGGVAFLDISTGEFYTSEGNLDYVDKLINNFK
                     PKEILVERTKYKEFLEQFGSKHYVTRLDEWAFNEDAAREKLTKHFKTQSLKGFGVESL
                     QFGITAAGAILYYLDVTQHTQIGHIQSISRIDEDSFVWIDRFTSRNLELFSSINEGAK
                     TFIEVIDKTSSPMGARLLKRWVALPLKNTDSINDRLNVVELFVNNEDMRATLTDKIRD
                     MGDVERIISKAAVGRIQPREMVQLKNALSLIDEVKTICDQSAEPTLLRISEQLNPCHR
                     IRERIEQEIVPDAPMMLGKGQVIAKGVNAELDELRDILYSGKEYLLNIQQRESERTGI
                     PSLKVNFNNVFGYYIEVRNTHKDKVPADWIRKQTLVSAERYITQELKEYEEKILGAEE
                     KISELEQNLYQNLVHSLAEYVGSIQLNAYLIAQLDCLLSFAHCAVEFQYNRPQVNLDD
                     VIDIRQGRHPVIERMLPAGEQYIANDVYLDTREQQIIIITGPNMAGKSALLRQTALIV
                     LMAQMGSFVPAQAASIGVVDKIFTRVGASDNLSLGESTFMVEMQEAASILNNISPRSL
                     VLLDEIGRGTSTYDGISIAWAIVEYLHEHPSARAKTLFATHYHELNEMEKSFPRVKNY
                     NVTIKEVNNQVIFLRKLVRGGSEHSFGIHVARMAGMPPSVVKRANEILAELESSTQRQ
                     SLSKPVDELGTHREGYQLSIFQLEDPVLKQIRDQIKKIDINNLTPIEALNKLNEIKRL
                     TGL"
     gene            complement(12131..15100)
                     /gene="secDF"
                     /locus_tag="PK049_09005"
     CDS             complement(12131..15100)
                     /gene="secDF"
                     /locus_tag="PK049_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HOK61738.1"
                     /translation="MQNKGAIRFIAIALALVCLYQLSFTYVTWRVESKAREYATVNNI
                     FDPEKYEHYLDSMSQEQVYNFLFVRKYNYKECKEREINLGLDLKGGMNVMLEVSVTDL
                     VKALSNNSQDSAFVKALNLANQMPGSEHFIDKFGKAFKQVAPNARLASIFNTPELRGR
                     INFDSSDEDVLKVLKQEAENAIDNSFNILRNRIDKFGVAQPNIQRLENSGLILIELPG
                     IKEPERVRKLLQGTASLEFWETYEYSELFNNFMAANSTLAEINKASQPTVTETTKKDE
                     TTEQKDELLSKLKETEKTDTLKAAADMAKDYPLFSILQPNLYNNQPAPGPVVGYAHIK
                     DTAQVNEILRMPKIRALFPPDVKFRWGVKSPKWDKNKVFYELIALKVTTRDGKAPLDG
                     GAITDAREAFGDRKGEAEVDMEMNAEGARIWARMTGENVGRCIAIVLDDYVYSFPRVQ
                     TEIKGGRSQITGDFTINEAKDLANVLKSGKLPAPAHIVQEQLVGPSLGKEAINSGMNS
                     FIVSFIIVLLYMVIYYSRSAGLVADFALVTNLFFIMGVMASFGATLTLPGIAGIVLTL
                     GMAVDANVLIFERIREELRGGKGLSLAVKDGYRNALSAIIDGNVTTLLTGVILYLFGS
                     GPIRGFATTLMIGIITSMFCGIFITRLFLLWMEKRNIPMIFSTKLSENAFQKVKIDFF
                     GLRKKAYFFSGTLIAIAIISLAVRGLNPGIDFAGGCSYIVRFQNTVSTQEVSKSLKTY
                     LGEAPEVKTIGASNQVKITTKYKVADNDPNVDNEVDSLIYLGVKPFMANGANFSDFQH
                     DYKQSSIKIGPTIAHNIKRDAYIAVFFALVVIFVYILIRFRYWSYSAGGVIALFHDSL
                     ITIGLFSILYYRVPFNMEIDQSFIAAILTIIGYSINDTVIIFDRIREYMGLHPKRTRR
                     ENMNSAINDTLSRTFSTSFTTLIVLIPMFFFGGEVIRGFVFALIVGILIGTYSSIFIA
                     APIAYDLEKRKEDKKNK"
BASE COUNT         4183 a         3336 c         3715 g         4003 t
ORIGIN      
        1 cccttacgcc ccgcgggatt accccgcggg gcttttttat tttggtaccg gcccaagcct
       61 aacgctacgc ctcagccaga gcataaaaag ctttttgtac gagcctgaac caaggggatt
      121 ttttactaca tttactgaca gctaccgggc ggagcggcgt atgccgcacg cgtttaaaaa
      181 cgagcgggag cgagggtaat aaagcgaaag aagtaattgt cacggactac aagtccgcgc
      241 cagcaattgg ggaatatgaa aaaggatgaa taaaaaagga agttataaaa taaaatatta
      301 ttgtttagcc cctgttcagc cgaggctgta aaaagaaata aacgaaccgc gttcggcggc
      361 ggttgcaccg ccgtcgtgct atcagcaaca ggctgcacct gagtttttta taattccccc
      421 cagcacccgc tcgcgcggat ttaccttcgg tgagctccct tcggtcggtt gtaatccgtg
      481 tgattgcagt tcggcaaagc caatccttgc ggttatttta agaggtgttt tcaatgaggc
      541 acagctcgaa taagtaataa agtgtgtaag aagtatttgt cacggactaa aagtccgcgc
      601 cagcaatgaa tggcgtacgc cgcacgcgtc catagacgcg cgcgaatgag aggaaacggt
      661 tcacggttaa cgactcacgt ttaacggact tcatctccct ctaacttcct catattccgt
      721 ctcattccga cccagcgcta acgctagttt gtttgttctg tttcaatctt ttttgtgtta
      781 cttagcattg tttaacgcgt atcattcata tatttgcaat cctaaaatag ctggtttgaa
      841 acagttttta tcagttccat ttgcttgcct actgcttgtt gtggcggtgt atatatcgag
      901 cagctacgcc tatccgcagg agggggctcg tacactagcc ggtaaacact cccagagcga
      961 ttcgctaagg ggtaggaatg tcccttttga taagggtagt gcaaggggtg taggcaggtc
     1021 agctactgat acgctaaaac ccaatgagcg caacatttac ggttaccttg gcattcaaga
     1081 ccctaagagc aagtcgtttg cctggtattt tgaccgggaa acctatgatt tggttcaaac
     1141 acaagccttt gacagctcgc tatttttggt gcacctgata ctacccgggc aaaagagcct
     1201 atcgccgtta acctacctgg gcaacatggg ctcgcctgtg cagagtgacc atttttttga
     1261 acggccggtt agctatccgt tcctgttttc aaagggctat tccagctacg agcagccggt
     1321 gctggatagg aagcagtaca acgtacgacg cccgcatacg ctgctggagt attcaactgc
     1381 cggcaaaagg cgtaatgctg agcaaaacct tagggttttt catacgcaga atgtgaacag
     1441 gtacctgaac ttcgggttgc agtacgacta cttcagcacc aagggtatat acgaaaggca
     1501 gctaacccgt aataacgatt ttactgcctt tgcaagctac tataggcata gggtatcggc
     1561 acagggaacg tttgcctaca catacattcg gaatcaggaa aatggcgggc tggaggacga
     1621 taggttcata caggataccg ttatggagcc caacctggtg cctttcaggc ttaaggatgc
     1681 atcggttgag taccgtaaaa ggagcttttc cggagtggtg ggttacgata ttattacaaa
     1741 aagggtaaac gatagccttg aaaatctgaa atcgattctc accgccaagc tgatacttga
     1801 tgccaaccgc tacacacggg tttacgccga taccgaaacc gattcatcgt attacggcaa
     1861 cttctatatc agcaccacat ctacccacga ctcggtgtac ctagccacct accagtcgac
     1921 cctgcttgtg gagctaagcc aaattgccaa atatccgggc atacccggat tacgggcatg
     1981 ggtatcgggc ctaatgggga gctactacaa ctttacaccc gataacttta tatataccta
     2041 cagcaatacc aagctcaaca ccagccactt tggcctgggg gtttacagcc aaagccctta
     2101 cctatcgtac agtggcgctg cacgttttta catgagcggg tacagggcag ccgataagga
     2161 gttgcttggc cgtttatcca tatcgccatg gaaatcgaag gagctgcctt acgtagcagg
     2221 ggagctaagc attaccgata gggaacccga tatctttata aatcgatatt tttcaaatca
     2281 ttacaaatgg gacaacactt ttgaaaagga gagccgtttc aggttaagcg cacgccttgg
     2341 agccgatagg tttggttttg aggcagggta tagcattgag catatcctta actatgttta
     2401 ctttgacacg ctctcgttac ctgcccagga acctaaggtt acggtaacct cggcctacat
     2461 tcagaaaaaa ttcaggctcg gttggttcaa cgctgtgtgt aaggttattt ggcaggcaag
     2521 caccaatgcc gatgttttaa gcttacccac ccttgcggga tttggtgcac tttacgtgca
     2581 gtaccccgtg gtaaaggatg tgcttaccat gcaacttggt gttagcggat tctaccgtac
     2641 cgagttttac gccgatgcct ataatccagc acttggggtt taccacaacc agcgcgttcg
     2701 aaaaattggc aactatccgt ttgtcgatgt attcctgaat gcccggtgga aacgtgccaa
     2761 tttatttatt aagcttgacc atgctaacca gggattaatt gataatcagt attttacaac
     2821 gctacactac ccgtacaatc cacgtatggt taagtttgga gtttcgtgga tgttttacga
     2881 ctagtcgtca aaattctcaa ttgtaattca acactttagg ctaattttgt gtttctgttt
     2941 ggttaaacca aaaaagtcat attatggttc gaaaagttat ttacctgcgt aatgggttga
     3001 tttttttggc tatgctaatt tttttctcgg tcagtagctg tgagaaaaaa ggggttaagg
     3061 acgaggaagt tcagcgagac cttgccgata tactggccga tggcaaactt gttgctgtaa
     3121 ccgattataa cgcaacaagt tactttatct acaagggtga accaatgggt taccagtacg
     3181 atatgcttcg cgatttagct acctatttag gggtaaagct cgaaatcgtg acagagaacg
     3241 atatcgacaa atcgtttgag atgctcagga agggcaaggt tgatatcata gccagcagct
     3301 tagccataac agcccagcgc aaggaggagg ttgattttac cttgcctcat ggcgaaacca
     3361 gccaggtgct ggttcaaagg atacccgctg aaaacggcac tcctgccatt caaaaaccgc
     3421 ttgaactatc gggtaggttg gtttacgtgc aaaaaagctc agcaggcgca cagcgcctaa
     3481 aaaacctatc cgatgaaata ggtgggggaa taaccgttgt agagctcccc aactacgatg
     3541 ccgacaagct gattgagctg gtagctacag gggaaatcga ttatgcagtt tgcgaccagt
     3601 ccgtagccct ggtaaaatca aactactaca agaatatcaa catagatatt caaataggat
     3661 tcccgcaaaa aacggcctgg gctgtaagga aaacctcggt tgagctccgg aaaaaagttg
     3721 atacctggct ggagggttac ctaaaaacgg ctcactaccg taatattgag ctaaagtatt
     3781 ttgcccagga acgctttaac aggcgcgcca gcagcgatta ctttttcatt tacaccggca
     3841 agatttcgcc gtgggacgag tactttaaga agtacagcca gggcattgac tgggactggc
     3901 ggcttttagc ctcgctggtt taccaggagt cgcgcttcag gcacgatgtt acctcgcaca
     3961 ggggcgcatc ggggttaatg cagatgatgc cccaaaccgc cgagtatttc ggagttgata
     4021 gtacggtagg gcctgccaag caaattgagg ccggcgtgag gtatatcaag tggctggaag
     4081 accgatttgc cgcttacgat attcccaagg acgaaatggt aaagtttgtg ctagcatcgt
     4141 acaatgcagg gataggccat ataattgacg cccgaaacct tgcccagaaa catggtcgaa
     4201 accccaatgt ttgggacaat aacgtggagt actttatccg gaataagtcc cagtttgtaa
     4261 acgaccccgt tgttaggcac ggcaagctaa agggggttga aaccacaaac tacgtgaagg
     4321 agataatgga aaggtaccag cactacaaga atatcatagg agagtaaagc tctccttttt
     4381 tttgctgtaa cctgctttat tcatacattt gcgcaaaatt taagatgaaa atgagtaatc
     4441 gtagagttag ggtgcgtttt gcaccaagtc ctaccggccc tttgcatatt ggaggagtgc
     4501 gtacagcgct attcaactat ttttttgccc gccagcacgg tggcgatttc attctcagga
     4561 ttgaggatac tgactcgcaa cggtttgtcc ccggcgccga ggagtatatt aacgaggcgc
     4621 tgaactggtg cggaataaag atagatgagg gcgttcgtga gggtggcccg catgcgccct
     4681 accgccaaag cgaacgcaag cacatataca agcagtatgc cgataggctt gtggagagcg
     4741 gtcatgcata ctatgccttt gacacacccg aggtgttgga caaaatgcgc gccgaggccg
     4801 aagcccaagg cggggctttt acatataact acgccattag gaatgggatg gagaattccc
     4861 tggcactacc agccgatgag gttaagcgcc gcattgagcg tggcgaccaa tgggttattc
     4921 gttttaagat gcctgagaac gaggaggtgg tgatgcacga ccttatccgc ggcgaggtta
     4981 gggttaacac atcaaccctt gacgacaagg tgctgtttaa gagtagcgat atgctaccca
     5041 cctaccacct ggccaacgta accgacgatt accttatgga gatttcgcac gttatccgtg
     5101 gcgaggagtg gctaccatcg ttgcccctgc acgtgctgct ttaccgagcc ctgggctggg
     5161 agaatgtgat gccaaagttt gcacaccttc cgctactgct aaagccatcg gggaatggca
     5221 agctgagcaa acgcgatggc gataaaatgg gattcccggt tttcccgctg ctctggaaaa
     5281 gcgccgatgg cgaaatatcg cgtggttacc gcgaggatgg ttactttccc gaggcatttg
     5341 tgaacctgct tgcccttttg ggatggaatc ccggaaccga ccaggagctg ttcagcatgg
     5401 atgagctcat aaagctcttc agcattgagc gcgttaacaa atcgggcgca cgtttcgacc
     5461 cggaaaaggc aaaatggttc aaccaccagt accttatccg caaatccgac gccgaggttg
     5521 ctgagctatt ccagcctgtt cttgaacagt acaatatcga tgtaccattc gacaagctca
     5581 cgcgaattgt tgggctggtt aaggagcgcg ttaactttgt gcatgagcta tgggggcaaa
     5641 caagctattt ctttgaacgc cacctggagt acgaccagca agctgttgcc aagcactgga
     5701 agcccgaaac acccgatatg ataaccgatg ttagcgaaac gctaaaagca ttggatacct
     5761 ggaactcgca cgagattgaa acagcgctta aagaccttat caacgctaag ggttacggca
     5821 tgggcaaggt tatgaacgct gtaaggcttt gccttgttgg cgaatcgaaa ggccctggga
     5881 ttgccgatat ttgcgaaatt ctgggcaggg atgaaaccct tacaaggctt aaccaagcca
     5941 ttttaattct gaatggtaag aagtgaatcg gaatgaatcg gaatgaatcg gaatgaatcg
     6001 gaatgaatcg gaatgaggcg gaatgaggcg gaatgaggcg gaatgattcg gaatgactcg
     6061 gaatatgagg aagttagaag aagttagaag aagttagaag aagtaagagg aagttagagg
     6121 aaatattagt tcgttaaacg tgatccgtga aacgcaatgt ttgaggcggg aatgagtcgg
     6181 aatgagtcgg aataaacaga atatgatgga gttagaggaa gttagaggaa tagtaaaatg
     6241 tgaacggctg aaccaaatga ccttgaatta acaaatttaa ccgggctaaa taataggaac
     6301 gcataattat ttgaggagat tagccagaag atgcacaaaa aacatttaat tattgtttca
     6361 gttgacgaat tcggatttaa ttatctttgt ggtatgagaa acattgtaag catagctgca
     6421 acctgttgtt gtatgtggta taggtttgta ccgcagggga attgctatgc gcgatgttga
     6481 ttactagtta actcaatcca taactcaagc ccttccggta catacacgga ggggcttttt
     6541 cattttatat ggcttttagc tactaaaacg ataaacagat tgattttatg gaaaggaaca
     6601 aacttaacta cattgaaacc cgccgaatgg ttgaggactt aagcgatagc cttacagagc
     6661 tggttgttga gagtttgctt ggtgccggac agacggttga tacagaaaag ccttacagta
     6721 aactttacga ggcgttacgg tattttaatc ccgataccag tcaatgccaa aggcaattgg
     6781 aaattgttta ccaaggattc caaggtgtcc ttgatacact tagccacgat gcaaacttta
     6841 tctacctgaa cgacccagca gcacaaagcc ttgaggaggt actgcttgca taccccggat
     6901 tttttgccat tgcccattac cgccttgcaa acctactgca tagggtaggg gtaaacattt
     6961 tgccaaggat gattaccgag tacgcacata gtaaaacagg cattgatatt catccggcag
     7021 caaccattgg gcaggagttc tgtatcgacc atggaacagg agtagtgatt ggcgaaacaa
     7081 ccataatagg ccagcgggta aagctgtacc aaggcgtaac cctaggcgcg ctaagcgtat
     7141 cgaagagcct ttcggccaca aaacgccatc ccaccattga ggacgatgtg attatttacg
     7201 caggcagcac catacttggg ggcgagaccg tgattggaca tgatagcgta attggcggga
     7261 atgtatggct tatcaaaagc gttccgccct tctcaatggt ttaccatgag agcaaagtga
     7321 ttgtacaaca gggaaagttt aaccataaaa tgaagtgata gatgaaatac ttgagtatcg
     7381 ataaatcaat tggagcaacc ccacacatta ggcttagcag gcttttccca aacgctgagg
     7441 tttggcttaa ggatgagcgg cgcaatccga gcggtagtat caaggatagg gctgccatgg
     7501 caatggttga ggatgccgaa aggaaaggac ttttaaaacc gggtagtgtt atagttgagc
     7561 caacatcggg taataccggg attggccttg ccatggttgc ggctgtaaaa ggctataggt
     7621 tgatactgac catgcctgag agcatgtcgg ttgagcgaag ggtaatacta aaagcttacg
     7681 gagccgagat tatactaacg ccagcagcgc agggtatgca gggcgcaatt gataaagcaa
     7741 aggagtttat ggctcaactg ccatcggcat ggatgcccat gcagtttgcc aaccctgcca
     7801 atccaaacac acactcactt accacagcca aggagatttt agccgatttt ccggatggac
     7861 tcgatatggt tgtggccggg gtgggtactg ccgggcatat caccggcgtt ggtcgaacac
     7921 ttaaagaagc attccccaac attcaggtaa ttgcagttga gccgttcgac tcgcctgttc
     7981 tctccggtgg gcaacgggct ccgcacccca ttcaggggat tggggctggc tttgtgcccc
     8041 aaaacctcga ccgtggtgtg atagatgaaa taattacggt aaaagccgat gaggcgattg
     8101 aatgcatgcg gttacttgcc cgaaccgaag ggattttagc tgggatatca accggagcaa
     8161 atattgctgc cgttaaaaaa ctgctgtcag taaagcagtc caacctaaga attttaacct
     8221 ttgcctacga tactggtgag cgctacctga gtgtaaaggg tatttgggat taggtttaaa
     8281 gcccggtaag gcgtttaatc tcattcagct tattcagcgc ctcaatggga gtaaggttgt
     8341 tgatatcaat ctttttaatc tggtcgcgta tttgctttag tacggggtcc tcgagctgga
     8401 agatgcttag ctggtagccc tcacggtggg tgccaagctc atcaaccggt ttgcttagcg
     8461 attggcgttg ggtggaactc tccagctcag caagtatctc gttggcgcgt tttaccacgc
     8521 tcgggggcat accggccata cgggctacgt gtattccaaa gctatgctcg ctgccacccc
     8581 taaccagctt ccgcaggaag ataacctggt tgttgacctc ttttatggtt acattgtagt
     8641 ttttaacacg tgggaacgat ttttccatct cgttcagctc gtggtagtga gtggcaaaga
     8701 gggtttttgc ccttgcgctg gggtgttcat gcaggtactc aacaattgcc caggcaattg
     8761 aaatgccatc gtaggttgat gttccacgac caatctcatc gagtagcacc aggctccggg
     8821 gtgagatgtt gttcaggatg ctggcagcct cctgcatctc caccataaaa gtcgattcgc
     8881 caagcgacag gttatccgat gccccaacac gagtgaatat cttatcgact acaccaattg
     8941 aggccgcttg ggcgggaaca aacgagccca tttgtgccat aagcacaatt aaggcggttt
     9001 gccttagcaa tgccgatttt ccggccatgt tgggtccagt aattatgata atttgctgct
     9061 cgcgtgtgtc gaggtaaaca tcgttggcta tgtattgctc gcctgccggc agcatgcgct
     9121 caataaccgg atgtcgtccc tgcctaatat caattacatc gtcgaggtta acctgggggc
     9181 ggttgtactg gaactcaact gcgcagtggg caaatgatag caggcagtcg agctgagcta
     9241 tgaggtatgc gttaagctgt atggagccca catactcggc aaggctgtga accaggttct
     9301 ggtaaaggtt ttgctccagc tcggaaatct tttcctcagc gccaagaatt ttctcctcat
     9361 actcctttag ctcctgggta atgtagcgct ccgccgatac aagggtctgt ttcctaatcc
     9421 aatccgctgg aactttgtcc ttgtgggtgt tcctaacctc aatgtagtag ccaaaaacgt
     9481 tgttgaagtt aaccttaagc gatggtattc ccgttcgctc cgattcgcgc tgctggatat
     9541 ttaagaggta ctccttgccg gagtaaagta tatcgcgtaa ctcgtcgagc tcagcgttaa
     9601 caccttttgc aatcacctga ccctttccaa gcatcatggg agcatcgggg acaatttcct
     9661 gttcaatgcg ctcgcgtatg cggtggcagg ggttcagctg ctccgatatg cgaagcagcg
     9721 tgggttcagc gctttggtcg cagatggttt taacctcatc aatcaggctt aaggcatttt
     9781 ttagctgtac catctcgcgg ggctgaatac gacccaccgc agcctttgaa atgattcgtt
     9841 ccacatcgcc catatccctt atcttgtcgg taagggtagc gcgcatatcc tcgttgttaa
     9901 caaatagctc aacaacgttt agcctatcgt taatggagtc ggtatttttt aggggtaagg
     9961 ctacccaacg cttcagcagg cgggcaccca tgggcgatga ggttttatcg ataacctcaa
    10021 tgaaagtttt ggctccctcg ttaatggagc tgaacagctc caggttgcgc gaggtaaacc
    10081 tgtcaatcca tacaaagcta tcctcgtcga tacgggaaat gctctgaatg tgtcctattt
    10141 gtgtgtgttg ggtaacatca aggtagtaga gtatggcgcc agcagcggta attccaaact
    10201 gcaacgattc gacaccaaac ccttttagtg attgggtttt aaagtgtttt gtcagttttt
    10261 cgcgagcagc atcctcgtta aaggcccact cgtcgagccg tgttacataa tgtttgcttc
    10321 caaactgctc gaggaactcc ttgtactttg ttcgttcaac aagtatctcc ttgggcttaa
    10381 agttgttgat aagcttatcg acatagtcca aatttccctc ggaagtgtaa aactcaccgg
    10441 tggatatatc aaggaaagcc actcctcccg cttttttatc gagatagaca caagccagga
    10501 aattgttctc cttacgctcc agaacattat cgttgtacga aacgccgggg gtaaccagtt
    10561 cagtaattcc cctttttact atggttttgg cttttttggg gtcctcgagc tgttcgcaaa
    10621 ttgccacctt ttggccggca cgtacaagct ttggcagata ggtgtcaagg gcatggtgtg
    10681 gaaatccggc aagttcaaca aacgatgcag caccgtttgc ccggcgggtt aaggttatcc
    10741 ctaaaatctc cgaggtttta atggcatcct caccaaatgt ctcatagaaa tcgccaaccc
    10801 tgaaaagcag tatggcatcg gggtggttac gtttgatgct caagtactgc ttcatcagcg
    10861 gagtttccac aatatttata tcattctcct tgctcaccgt ttattagttt tccggaacgt
    10921 ttacaaaaat accaaatagt tgcatagcaa ttctgccctg ttgaaaaaaa cgttaaaact
    10981 actttacaac ataaaattgc caggaatgag gattaaactt ttggctagtt ttgcgctcaa
    11041 ataaagcgaa acccccgact tcacagccgg ggatttcaaa tggttcaggt attagtaagg
    11101 attggaaata atcgcagtaa agttaaaaat tttcaacaac attttcaaca cttaggtttc
    11161 agtagaggaa cgctgtgatt gcttactaat acctaaccat atacaggaca ggcttttggc
    11221 ctgtcttttt ttatttagat tttttttagg ttttttagac cctaccgttc gatattagat
    11281 cttaatccaa tttcgtttat aaactccttt tgtcattctg taaaacgaag gatatattga
    11341 atagtgtgca gtatatagtg tttaggggtg tttggagttt agtgtttagt gaaatgcttt
    11401 ttttcactat caaccatcaa ctatcaactc tgcttgtcat cctgagcgca gcgaaggatc
    11461 tctgaccgac ctaatgagat gtttcgctag cgctcacatg acattagttt cacgaacaaa
    11521 gtttccagcc ccgtaggggc gtaatgtttg taaccctatg attactccca tgtgatgcga
    11581 tgcacgcaat actttttcca aagaacagct cagaaaggtt gcatcgcaat aaaaatgcat
    11641 ggcaaagagc gattgtttga aaaccaatca aaaggaataa aggattaaag gcacaaagag
    11701 atgcttcggc gagctcagca tgacaaagag aacgaggatg ccagaggttt gtaattccga
    11761 gctagtcgag gaatcacatt aaaaagaaat aaaggaggat atagatgctt cgactgggtt
    11821 cagcatgaca aataacatta actaaacaac attgaatatc actcctcttc gaggaattta
    11881 atccaagtac tatgaatttt gaggaatgag tcggaataga catgggtgat tttatgaagg
    11941 aatgaggcgg aatagacgga atatgttgat gtttgaggaa tgttaaaatg aacaccaaag
    12001 ccaaaaatac catgaattaa caaattcaat tgggcaaata aaaaaaggtg aggcggaaat
    12061 atttttaggg gagattagta aaagaaaaag gcgaccgtta gggtcgcctt tttttaagca
    12121 tcgatataga ttacttgttt ttcttatcct ctttacgttt ctctagatcg taagcaattg
    12181 gtgctgctat aaaaattgag gagtaggtac caattagaat accaacgata agagcaaaaa
    12241 caaaaccacg aattacttca ccgccaaaga agaacattgg gataagtaca atgagagtag
    12301 tgaacgatgt tgagaaggta cggctcaatg tatcgttaat agcgctattc atgttctccc
    12361 ttctggtgcg cttggggtgc agacccatgt actcacggat acggtcgaaa ataataaccg
    12421 tgtcgttaat ggagtaacca ataatggtta ggatagctgc aataaacgat tggtcaatct
    12481 ccatattaaa tggcaccctg tagtaaagga ttgagaacaa tccaatggtt attagcgagt
    12541 cgtggaaaag agctataaca ccgccggcac tgtagctcca gtacctgaac ctgataagga
    12601 tgtaaacaaa aattactaca agggcaaaga acacagcaat gtaagcatcg cgtttgatat
    12661 tgtgggcaat ggtaggccca atcttaatgg agctttgctt gtaatcgtgc tggaagtcgc
    12721 taaagttagc gccattggcc ataaacggtt taacaccgag gtaaatcaat gaatcaacct
    12781 cgttatctac attggggtcg ttatcggcta ctttgtattt ggtggtaatt tttacctggt
    12841 tcgatgcgcc aatggttttt acttcggggg cttcgccaag gtatgtttta agcgatttgc
    12901 ttacctcctg ggttgaaacg gtattctgga agcgaacaat gtatgaacat ccaccggcaa
    12961 agtcgatacc cgggttcaaa ccacgaacgg ccaacgaaat aattgcaatg gcaatcaaag
    13021 tgccggagaa gaaataagcc tttttacgta gcccaaagaa atcgatttta actttctgga
    13081 aagcattttc cgaaagtttg gtagagaaaa tcattggaat gttgcgtttt tccatccaaa
    13141 gcaggaatag ccttgtaatg aatattccgc agaacatcga tgtgataata ccaatcatca
    13201 gggtagtagc aaaaccacgg ataggtcctg aaccaaacag gtataggata acaccggtta
    13261 gcagtgtggt aacgttaccg tcgataatag cggagagcgc attgcggtaa ccgtccttaa
    13321 cggcaaggct caaaccttta ccaccgcgga gttcctcacg gatacgctca aaaataagta
    13381 cgttggcgtc aacagccata ccaagggtaa gcacaatacc tgcaataccg ggtagggtta
    13441 gggtagcacc aaacgatgcc ataacgccca tgatgaagaa caggttggtt acaagggcaa
    13501 agtcggctac taaaccagcg ctacggctgt agtaaataac catgtaaagc agcacgatga
    13561 tgaacgacac aataaaggag ttcattccag agttaatggc ttccttgccg agcgatggac
    13621 caaccaattg ttcctgaaca atatgagcag gagcaggaag tttacccgac tttagcacgt
    13681 tggcaaggtc cttagcctcg tttatggtga aatcgccagt aatttgtgaa cgtccaccct
    13741 taatttcggt ttgaacacga gggaatgagt aaacgtaatc gtcgagcaca atggcaatgc
    13801 aacgaccaac gttttcgccg gtcatacgtg cccaaatacg tgcaccctcg gcattcattt
    13861 ccatgtcaac ctcggcttcg cccttgcgat cgccaaaagc ttcgcgggca tcggtaatgg
    13921 ctccaccatc gaggggtgct ttgccatcgc gggtggttac tttaagagca ataagctcat
    13981 agaatacctt attcttgtcc cacttgggcg atttaactcc ccagcggaat tttacatcgg
    14041 gagggaataa tgccctaatc ttgggcattc tgagaatttc atttacctgg gcagtgtcct
    14101 tgatatgggc atatccaaca acagggccag gtgcgggttg attgttgtat aggttaggct
    14161 gcagaatgct gaataagggg taatccttag ccatgtcggc tgcggccttt aaggtatcgg
    14221 ttttttcggt ttccttaagt ttgcttagca attcatcctt ctgctcagtg gtttcatctt
    14281 ttttagtggt ttcggtaaca gttggctgac ttgccttgtt gatttcggca agggtgctgt
    14341 tggcagccat aaagttgttg aacaactcgc tgtactcgta ggtttcccaa aactcaaggc
    14401 ttgcagttcc ctgtagaagt ttgcgaacac gctcaggttc cttaataccg ggtaattcaa
    14461 taagaatgag tcctgaattc tcgaggcgtt gaatattggg ttgtgcaaca ccaaacttgt
    14521 cgatacggtt acgaagaatg ttgaaggagt tatcaatagc attttcggct tcctgcttca
    14581 gcaccttaag tacatcctcg tccgaagaat cgaagttgat tcgcccccgc agttctgggg
    14641 tattgaaaat tgaagccaaa cgagcattgg gagccacctg cttaaaggct ttgccaaact
    14701 tatcgataaa gtgctcgcta ccgggcatct gatttgccaa atttagcgct ttcacaaaag
    14761 cgctatcctg gctgttgttg gacaaggctt taaccagatc ggtaaccgac acctcgagca
    14821 ttacgttcat accacctttc aggtcgaggc caaggttaat ttccctttcc ttacattcct
    14881 tgtagttata ctttcgtacg aaaaggaaat tgtacacctg ttcctgcgac atggagtcga
    14941 ggtaatgctc gtacttctcg ggatcaaaaa tgttgttaac cgttgcatat tcacgggctt
    15001 tgctttcaac ccgccaggtt acataagtaa acgaaagctg gtacaagcaa accagcgcta
    15061 atgcaatggc aataaatcgg atcgcacctt tattctgcat cgttctaaag tattaaaaag
    15121 ttgataattt gccagttttc aaaaatatcg ggcaaatata atttttttta acttacaaac
    15181 agcctttagc tgttactcat tttcaccttt aacaggtttc aggtaaatgc gtttcct
//
LOCUS       DAOSAL010000066        14659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_2783_length_14659_cov_46.481854, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000066 DAOSAL010000000
VERSION     DAOSAL010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 14659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14659
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2783_length_14659_cov_46.481854"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="PK049_09010"
     CDS             <1..440
                     /locus_tag="PK049_09010"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOK61739.1"
                     /translation="NRSFDFPAKGKPTPAMAAVIELLDSIVKLSYIDRNRLYVGGLSM
                     GGMGTFELIARKPKMFTAAFPICGGGSPKNVKRYAKRVKIWVFHGEADDVVPLYHSQR
                     MVDAIKQAKGDVRFTVYPGVGHNSWDNAFAEPELLPWLFSKSK"
     gene            complement(494..1162)
                     /gene="clpP"
                     /locus_tag="PK049_09015"
     CDS             complement(494..1162)
                     /gene="clpP"
                     /locus_tag="PK049_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOK61740.1"
                     /translation="MREFEKYAIKHRGINSLTLHQYTSMSNGYISPTIIEERQLNIAT
                     MDVFSRLMMDRIIFLGVPINDDVANIIQAQLLFLESTDPSKDIQIYINSPGGSVYAGL
                     GIYDTMQYISSDVATICTGMAASMAAVLLTAGEKGKRTALPHSRVMIHQPMGGAEGQA
                     SDIEIVTREILKLREELYEIIATHSGNPIERIRKDSDRDYWMTAQEALEYGMIDEVLM
                     RNKK"
     gene            complement(1298..2644)
                     /gene="tig"
                     /locus_tag="PK049_09020"
     CDS             complement(1298..2644)
                     /gene="tig"
                     /locus_tag="PK049_09020"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOK61741.1"
                     /translation="MNIVREDIDSLNTTIRVKVEKNDYAESVETSLRNYRKKANINGF
                     RPGMAPMSLIRKMYYRYVIADEVTKLASDKLFEFIKENKIRTLGEPIPSEKQTPINWE
                     TDETFELAWDLGIAPEIDIKFTKKDKFPFYKILPNDEIRKSYIDNYRNRNGKYVNVEA
                     TDDKGLIKATLTELNDDESPRENGITVEGASISVALVADEAERKKLTGVKVGDVIVID
                     THKAFPNQADRSALFRVKKEELENVNPLFQITVTETLTFAPAELNQEFFDAVYGEGVV
                     NSEEEFHKKIEEEIERNLAYESEYRFGIDLKEKLLEKLKIELPKEFLIRWLVAINEGK
                     FTREQIENEFPMFEKDLKWQLISNKVAEEQNFTVTNEELEDFAMGYARSQFAAYGMSF
                     LPDEYIKRYATNILKNRDEVRKMQERILDKKVIEWFKANVNLDTKEITIDEFNKLK"
     assembly_gap    2678..2777
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2893..3270)
                     /gene="crcB"
                     /locus_tag="PK049_09025"
     CDS             complement(2893..3270)
                     /gene="crcB"
                     /locus_tag="PK049_09025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HOK61742.1"
                     /translation="MFRSILIVGIGGFVGSALRFLVSRYFQLHTLSNFPWGTLTVNIL
                     GSFIIGLVFGLSERSNLITPEWRLFLAVGFCGGFTTFSSLANDAFILLQGREFLYLSA
                     YTAASFFLGLLAIFAGRYIIQII"
     gene            complement(3304..3927)
                     /locus_tag="PK049_09030"
     CDS             complement(3304..3927)
                     /locus_tag="PK049_09030"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61743.1"
                     /translation="MNNAIANILKHDSVIAQLAGKLEVKPLPVTNDIYLDMLENIVSQ
                     QLSGRVATTIFNRFLDLFPDRYPNPNHLLKLDDIELRKVGLSGAKSTYVKAMAEFAMN
                     HDLNVDTIQQLPDDEVIKLLTQVKGVGVWTAQMLLIFSLGRNDVFPVDDLAIRQGMVE
                     LYHLKSDGKKLRHQLLQISERWAPYRTWGTRLIWAYVNSKKQNATRV"
     gene            3998..4648
                     /gene="upp"
                     /locus_tag="PK049_09035"
     CDS             3998..4648
                     /gene="upp"
                     /locus_tag="PK049_09035"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HOK61744.1"
                     /translation="MIHELGQQNSILNQFIAELRDVNVQNDFMRFRRNLERCGEIFAY
                     ELSKVLSYQTVQVQTPLGTADVSVPTDNIVLATILRAGLPLHQGMLNYFDKAQNAFIS
                     AYRKYDKDGTFRIQFEYISCPSIEGKTLVLIDPMLATGASMLLAYKALLEHGKPLHTH
                     IVAVIGSKEGVNYMKRNLSPSEATIWVGAIDDELTVKSYIVPGLGDAGDLAYGKKD"
     gene            4744..5259
                     /locus_tag="PK049_09040"
     CDS             4744..5259
                     /locus_tag="PK049_09040"
                     /inference="COORDINATES: protein motif:HMM:NF019819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HOK61745.1"
                     /translation="MKLKVMVIISLFFGTSCKSQDLQTVPELNLERYAGTWYEIARLP
                     NSFEKNLECITATYTIRPDGRVTVENKGVKADGSMSSIKGVAWVSDKNEPAKLKVRFF
                     WPFAGKYWVVYIDNDYRYALVGEPKRKYFWILSRTPQVDSLSYKQLVDIASQKGFDTQ
                     RLITVNQKCPE"
     gene            complement(5291..5788)
                     /gene="folK"
                     /locus_tag="PK049_09045"
     CDS             complement(5291..5788)
                     /gene="folK"
                     /locus_tag="PK049_09045"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOK61746.1"
                     /translation="MAKVYLSIGGNLGDREQLIDSAREAIAARIGTIKKSSSIYESEA
                     WGFESESNFLNQVLMVETSLSPAAIMLEISYIESKLGRERNGKGYTSRTMDIDVLFYD
                     HLILYTPELIVPHKQLHKRRFILEPLKEIAPDFIHPLFSITISEIAISCPDAGKVWKY
                     EPVNA"
     gene            5997..8285
                     /locus_tag="PK049_09050"
     CDS             5997..8285
                     /locus_tag="PK049_09050"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HOK61747.1"
                     /translation="MFKNILIALLLVAGSFSLYANDIPCRILIINSYHPGLSWTDSLN
                     KGFIDAVRGFEKPVEIFIENLDTKRFPENLNRKEQKIYILSRIQQIKPNLIALTDDGA
                     FILMESLLKEFSGLDTIPIVFMGVNQEKDYPKNFTGVLETIDIKANLEFIKQLQPNVK
                     RVYCVTDQTITGKVIGERIKSTLLKYNPGFEVQVIGNCSYYELVDTLSNLNDNEAILF
                     LLFNRDSKGNYFSYEAILDSLSRNVKAPIYGTWSFYLNHGIVGGAIISGYHHGKRAGE
                     LASLLLKGVSPKTIAPTDGPSDFIVDHLQLVKYSISQKLLPQGTLILNKNKRFIHLSR
                     GTTLVVAAIVLLLIVLLIFMGVYSRMRHKALRLQKYYNTLLEQKNQEVENSLKFAEEA
                     NELKSAFLANMSHEIRTPMNAIVGFSKLIKESSLLSPEEVNGYLDIIAENAQRLMRLI
                     NDIIDISKIDSKQLSIYKRPVQLGPLFANCYRAAEVELDRVNKNNIAIEMSFRPTDAA
                     LEVVTDPDRLQQVVMNLLNNAIKFTSSGRIVLGYSVIHDLLRVSVEDTGIGIDSQYHD
                     AVFERFRQIDGTLNREYGGSGLGLSICKGIVEAMGGRIGVDSRPEVGSTFWFEVPISL
                     ATPGQHVEVPVKASKSKLKNKVLLIVEDNEPSAILLREMLKGTGAVLLQAATGKEVAT
                     ILNDKVKIDIALVDVYLPDMSGYEVVTKIKQAQPNVRVIAQTANAMDTDRQKSLEAGC
                     DEHITKPIDKDKLIELLSMLLS"
     gene            complement(8291..9199)
                     /locus_tag="PK049_09055"
     CDS             complement(8291..9199)
                     /locus_tag="PK049_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOK61748.1"
                     /translation="MEQYYGELAALLTAFFWTVTALAFESASRKVGSLSVNLLRLGTA
                     FIFLSVYALISRGKAFPVDAGTHQWVWLSLSGLVGFVLGDLFLFQSYVVIGSRISMLI
                     MSLAPPMAAIIGWITMGETLTLKQAIAMAVVLAGIVLVVLERQSADENGNGKRVRFSY
                     PVGGILLAFGGALGQAGGLVLSKYGMAGYDVIPSVQIRVITGIVGFTLFFAFSNKWKN
                     LSEALRNGKAMKRLLIGAFFGPFLGVSFSLLAVKYTSTGVASALMSIVPVLIIAPAVV
                     VFKEKITAKELVGAVITVVGVALFFL"
     gene            complement(9321..9572)
                     /gene="rpsT"
                     /locus_tag="PK049_09060"
     CDS             complement(9321..9572)
                     /gene="rpsT"
                     /locus_tag="PK049_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010265138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOK61749.1"
                     /translation="MANHKSAEKRIRQTESRREHNRYYAKTTRNALKALRNTTDKAAA
                     MELLPKVSAMLDKLAKNNIIHKNKAANLKSSIQKHVNAL"
     gene            complement(9599..9670)
                     /locus_tag="PK049_09065"
     tRNA            complement(9599..9670)
                     /locus_tag="PK049_09065"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9635..9637),aa:Glu,seq:ttc)
     gene            complement(9761..10234)
                     /locus_tag="PK049_09070"
     CDS             complement(9761..10234)
                     /locus_tag="PK049_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61750.1"
                     /translation="MKRLLFILATVFVTYSAANAQDYKTGLGLRGGYPSGVTVKHFFA
                     QKSAVEGVLSFGWGGIGITGLYQLHNPIPDAPGLKWYYGFGAHFATANADKKNPFESE
                     YGSKVFLGADGVIGLEYTFKDAPICLGLDILPIFNIIESPGIWFNAGFSVRYTFK"
     gene            complement(10285..11649)
                     /locus_tag="PK049_09075"
     CDS             complement(10285..11649)
                     /locus_tag="PK049_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOK61751.1"
                     /translation="MRVHFIAIGGSAMHNLAIAMKLKGYQVTGSDDEIFEPSLSRLKN
                     HGLLPSHQGWNPSIITPEIEAIVLGMHARADNPELLRAKELAIKIYSYPEFLYEQSRQ
                     KTRIVVGGSHGKTTITSMIMHVLKHLNYKFDYMVGAQLKGFENMVSFSDDAPFAVFEG
                     DEYLTSPIDPRPKFHVYRPNIAIISGISWDHINVFPTFDIYREQFAKFIDCIEPNGKI
                     IYCADDPEVCRVVEESQRNDIKRIPYHTHPHGIANGIWSLDFNGTSIPLKVFGKHNMQ
                     NISAAKAACITLGVTEKQFYNAIETFEGASKRLQLLAQGTSTSVFLDFAHSPSKLKAT
                     IEAVRELYPARKLVVCFELHTFSSLTKDFLGQYNGTLTGADVAYVFFNPHALELKKLP
                     PIDPNDVIQGFGDPHPAVFTDANNLVDVLLQHNWGNSNLLIMTSGNLAGTDLQHLADE
                     VVKH"
     gene            complement(11687..12403)
                     /locus_tag="PK049_09080"
     CDS             complement(11687..12403)
                     /locus_tag="PK049_09080"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HOK61752.1"
                     /translation="MKKKKRNNQYILPVALFIGALAVWIIVRFISDPHHRYLIWGIDV
                     SAHTGKIDWEKVKDHKVDFAFIKASEGATLKDKSFRNNVTRAREAGIPVGAYHFFNFN
                     RRGIAQAQNFLRAIDGVELDLPPVVDVEEWGNVVRRPRKQIIADLVDFVELVEKTTGQ
                     KVLIYTNKDTFKWYISETFPDHDIWICSFDHTPEIEKSWTFWQYHHEGRLKGVQGKVD
                     FNVFYGNTIEWKNYLNNKKR"
     gene            12532..14460
                     /locus_tag="PK049_09085"
     CDS             12532..14460
                     /locus_tag="PK049_09085"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOK61753.1"
                     /translation="MKFRIVKAIALNIPLLLILFVAQSGFISAQDDLLEGVKPSSLEA
                     LAKVLERPDDSVKVVTLKLLCWENRNSDPDKAIEYGQMAVSLAKSLNLKMELSDSYNR
                     LGIAYRNKGRYAQALECYFNGLEVSRTHGFDKHLAFQYNNLADLYNRLGLYGRALEFG
                     KRAMEYAQKVNDDYTLAYIYNIMGTIYKNKNELDSALNCFTHSLELRQKIGYNQGIAT
                     SHLNIGSIELLREQYSKSLQSIQTAIDLYSPKNDLQGIAIAYYYKGQLYNKLGNFNNA
                     IYCFDESLKYNESFKNLTVSRDCYNGLAYAYAKKKLFDKAYYFSSKATEINDSISLSQ
                     YVERLTQLTEALKYEETLNLQKEREKALAERLDFQRNIIKLYIAIIILLALIVGLVIY
                     FYIKRAKDIRLLQAQRDEINMLNSTKDRFFSILAHDLKNQLTSIVTIAQMVKDKSLAI
                     GDDGLIELSRRLYALGFATNDLLENVLSWIKSQGKQVIARKSNIDLKEFIDRVILTQK
                     PAAEIKSVAINNRVEPGISIHTDADMLSTVVRNLISNAIKFSSSGGRVDVFAFPKGNH
                     VEISITDYGVGMPRETTDRLFNTLESLTQPGTMNEQGSGLGLIICNQLVTDLGGQIKV
                     TSEPQRGSTFTVSLPHVS"
     gene            complement(14453..>14659)
                     /locus_tag="PK049_09090"
     CDS             complement(14453..>14659)
                     /locus_tag="PK049_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018694987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HOK61754.1"
                     /translation="LEFIVKPGAPITKGKLKDIGFPKDAIVGGVVRGDNAFIAKGDTE
                     IKPYDRVVVFALPTAINKVGKYFN"
BASE COUNT         4191 a         3046 c         3200 g         4122 t
ORIGIN      
        1 gtaatcggtc ctttgatttt ccggctaaag gtaagccaac cccagcaatg gctgccgtta
       61 ttgaacttct ggattcaatc gtaaagttga gctatattga taggaatagg ttatacgttg
      121 gcggactatc aatgggagga atgggtacct ttgagctgat tgcccgaaag cctaaaatgt
      181 ttactgctgc atttcccatt tgtggtggtg gtagtcctaa aaatgtaaaa agatatgcca
      241 aaagggttaa aatttgggta tttcacggcg aggccgacga tgttgttccc ctataccatt
      301 cccagcgcat ggtcgatgcc attaaacagg ctaagggcga tgtgcgcttt acggtttatc
      361 ctggagttgg gcataatagc tgggataatg cctttgctga accagagctg ttaccctggt
      421 tgtttagtaa aagcaagtag atactaaaaa aggctgcata aaatgcagcc tttaattttt
      481 ttcgggttcc aaattacttc ttattacgca taagcacttc gtcaatcata ccgtattcaa
      541 gtgcttcctg tgcggtcatc cagtagtcac ggtcggagtc cttacggata cgttcaatgg
      601 gattgcctga gtgggtggca ataatctcgt aaagttcctc gcggagctta agtatttcgc
      661 gggtaacaat ctcgatatcg gaagcttgcc cctcagctcc acccatgggc tggtgaatca
      721 ttacacgtga gtggggtagg gcggttcgtt tccccttttc gcctgcggta agcagaacag
      781 ctgccattga tgcagccatg ccggtacaga tggttgccac gtcggagctt atgtactgca
      841 tggtgtcata aattcccaag ccagcgtaaa cactcccacc gggtgagttt atgtagattt
      901 gaatatcctt cgatggatcg gtcgattcaa ggaatagcag ctgtgcttga atgatgttag
      961 ctacatcgtc gttaatggga acacccagga aaataatcct gtccatcata aggcgcgaga
     1021 acacgtccat ggtggcaatg ttgagctggc gctcctcaat gatggttggt gagatgtagc
     1081 cgtttgacat cgatgtatac tgatgtaggg ttaggctgtt aataccccgg tgttttattg
     1141 catatttttc aaattctctc atagccttta attttttgta ttagtaacgc aaaatactca
     1201 catttgttaa aacaaaaaag cggagtggct acaatttact aacaaaccaa aaaataaaaa
     1261 gccggtttac aaaccggcta tgctatgttt tgctttatta cttaagcttg ttgaactcat
     1321 caatggttat ctccttggtg tccaggttta cgttagcctt gaaccattca ataacctttt
     1381 tgtccagtat gcgctcctgc attttacgaa cctcgtcgcg gttcttgagg atattggttg
     1441 cgtaacgctt gatgtattca tcgggtagga agctcatgcc gtaggcagca aactggctgc
     1501 gagcgtatcc catggcaaaa tcctcaagtt cttcgttagt aacggtaaag ttctgctctt
     1561 cggcaacctt attgctgatg agctgccact taaggtcttt ttcaaacatg gggaactcgt
     1621 tttcaatctg ctcacgggta aacttgccct cgttgatggc aaccaaccag cggattagga
     1681 attccttggg aagttcaatt ttaagtttct cgagcagctt ttcctttagg tcgataccaa
     1741 agcggtattc gctttcatag gcaaggttgc gttcaatctc ctcctcaatt ttcttgtgaa
     1801 attcttcctc gctgttaaca acaccctcgc cgtaaaccgc atcaaaaaac tcttggttaa
     1861 gctctgctgg ggcaaaggtt aatgtttcag taaccgttat ctggaatagg ggattaacgt
     1921 tttccagttc ctccttctta actcggaata gcgccgacct gtcggcttgg ttggggaatg
     1981 ccttgtgggt atcaataaca atcacatcgc caaccttaac accggtgagc tttttgcgtt
     2041 cagcctcatc ggcaacaagg gcaacggaaa tagacgcacc ctcaacggtt atgccgtttt
     2101 cgcgtggcga ttcgtcgtcg ttgagttcgg taagggtagc cttaatcagc cctttatcat
     2161 cggtagcctc aacgtttaca tacttgccgt tgcggttgcg gtagttgtcg atgtaactct
     2221 tgcgtatctc gtcgttcggt aaaatcttgt agaatgggaa tttgtccttt ttggtaaact
     2281 tgatatcaat ttcaggggca atacctaaat cccatgcgag ttcaaaggtt tcgtcggttt
     2341 cccagttaat aggggtttgt ttttcgcttg gaatgggttc gccaagggtt cggatcttat
     2401 tttccttaat aaattcaaaa agtttatccg aggccagctt ggttacctca tcggctatga
     2461 catagcggta gtacatcttg cgaataagcg acataggggc cattcccgga cggaaaccat
     2521 tgatgtttgc ctttttgcgg taattcctca ggctagtctc aacactttca gcgtaatcgt
     2581 ttttctcaac cttaacccgt atggttgtgt ttaacgaatc gatatcttcc ctaacaatat
     2641 tcatactatg tgaattgatt tagttgcttg atttattnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnngcc atatcgcttt gcttggtaaa caatggtttc aaaaagaagc
     2821 gaatgcttca gcttatcggt tgagctaatg tagatgcgta gtcgtagagc tttactgaaa
     2881 ttttccatag tgttagataa tttggatgat gtagcgaccg gcaaaaattg ccaacaggcc
     2941 caaaaagaag ctggctgccg tgtaagcaga aagatacaaa aattcgcggc cttgaagcag
     3001 tatgaaggca tcgtttgcca atgatgaaaa ggtggtgaat cccccacaaa agcccactgc
     3061 caagaacaaa cgccactcag gcgtgattag gttgctcctt tccgaaagcc caaaaaccaa
     3121 accgattata aagctgccca aaatgttaac cgttaaggtt ccccatggaa aattactaag
     3181 tgtgtgcagc tggaagtatc tactcacaag gaaacgcaac gcacttccca caaaaccacc
     3241 tatgccaact attaaaattg acctgaacat gaatttcttt ttgcgaagat acaaaactat
     3301 tgcctaaaca cgagtagcat tttgtttttt gctatttacg tatgcccaaa tcaagcgggt
     3361 tccccaggtg cggtagggcg cccatctctc tgatatttgc aataattggt gccgaagctt
     3421 tttcccatcg cttttaagat ggtaaagctc aaccattccc tggcgaatgg caagatcatc
     3481 aacgggaaaa acgtcgtttc ggcccaggga gaagatgagt aacatctggg ctgtccacac
     3541 cccaacaccc ttaacctgtg ttagcagctt aattacctca tcatcgggga gttgctgtat
     3601 ggtatcaaca tttaaatcgt ggttcattgc aaactcagcc atagccttaa cgtaagtgga
     3661 ttttgcacct gataggccaa cctttcggag ttctatgtca tcgagcttta ggagatgatt
     3721 cgggttaggg tatctgtcag gaaataaatc cagaaagcgg ttgaaaatgg tggtagccac
     3781 cctgcccgag agctgctggc ttacaatgtt ttccagcata tcaaggtaga tatcgttggt
     3841 aacaggcagg ggtttaacct caagcttacc agccagttgg gctattacgc tgtcatgctt
     3901 aaggatgttg gctatggcat tattcatggc ttactaaaaa aaagaattgc aaagtaggtt
     3961 gatatttttg taactttgct aagataaaac atttccgatg atacacgaac ttggtcagca
     4021 gaactcaatt cttaaccagt ttattgcaga gttaagagat gtaaatgttc agaacgattt
     4081 tatgcgtttc cgccgcaatc ttgaacgatg cggcgaaatt tttgcctacg agcttagcaa
     4141 ggtattgagc taccaaaccg tacaggtcca aaccccactt ggaacagccg atgtttcagt
     4201 tccaaccgac aacattgtat tggccaccat tcttcgtgcc ggcttacccc tacatcaggg
     4261 aatgttaaac tatttcgata aggctcagaa cgcctttatt tcggcttacc ggaaatacga
     4321 taaggatggt accttccgca ttcagttcga gtatatttcc tgtcccagta tcgagggtaa
     4381 aaccctggta cttatcgacc ccatgttagc cactggcgca tcgatgctgt tggcatacaa
     4441 ggccttactt gaacacggta agccgctaca tacgcatatt gtggcggtaa ttggttcaaa
     4501 ggaaggggta aactacatga aacgtaacct ttcgcccagc gaggcaacca tttgggtagg
     4561 tgctattgac gatgagctca cagtgaaatc gtacattgtt ccaggccttg gtgatgcagg
     4621 cgatctggct tacgggaaaa aggattaagc gaatgtttta cttttggtgt ttagtattct
     4681 gattaataaa acaccgccgc attcagattg ctgaattcaa tattctaact aataaactcg
     4741 accatgaaac ttaaggttat ggtaatcatc tcattatttt ttggcacatc ctgcaaatcg
     4801 caggatttgc aaactgttcc ggaacttaac cttgaacggt acgccggtac atggtacgaa
     4861 atagctcgcc tacctaacag ttttgagaag aacttggagt gcataacagc cacctatacc
     4921 attcggcccg atgggcgggt taccgttgaa aacaagggag taaaagccga tggcagcatg
     4981 agcagcatta aaggagtggc ctgggtttcc gacaagaatg agcctgctaa gctaaaagta
     5041 aggttctttt ggccttttgc aggcaagtac tgggtggttt acattgataa cgattaccgc
     5101 tacgcgcttg tgggggagcc caagcgaaag tatttctgga ttctatcacg tacgccacaa
     5161 gtagattcgt tatcctataa gcaactggtt gatatagcaa gccaaaaagg attcgacacc
     5221 caaagactga ttacagtaaa tcaaaaatgc cccgaatagg ggcattttta gctattacga
     5281 taaatatgtc ttaggcatta acgggctcgt acttccatac ctttccggca tcgggacagg
     5341 aaatggcaat ttcgctaatg gtgatggaga acagggggtg gataaagtca ggtgcaattt
     5401 ccttaagggg ctcaagaatg aacctacgtt tgtggagttg cttgtgggga acaatgagtt
     5461 caggagtgta tagtatcaga tggtcgtaga atagcacatc gatatccatg gtgcgcgagg
     5521 tatagccttt accattacgt tcgcggccca gtttcgattc gatatacgaa atctcaagca
     5581 ttatggctgc tgggctaagg cttgtttcaa ccattaatac ctggttcaga aagttgcttt
     5641 cactttcaaa gccccaggct tccgattcgt atatggatga cgatttcttg attgttccaa
     5701 tccgggctgc aattgcctcg cgggccgaat caatcagctg ctctctatca cctaaattac
     5761 caccaatcga taggtaaact ttagccatga ctttttgggt tagtgtagca aacataccct
     5821 tttttttaaa aggctattca cctttaacaa aattttttta aaccgaaatt aacatgttgt
     5881 taacaatcag ccttatgggc aaaagggggt tacgctttga tcacaaaata ttaatggtaa
     5941 aaggaatttt atttattttt tcgtaaattg gggtattcaa aatttttgat acttccatgt
     6001 tcaaaaatat tttgatagca cttttactgg tagcaggtag tttttccttg tatgccaacg
     6061 acataccatg taggattctt atcattaact cttaccatcc gggtttaagt tggaccgata
     6121 gtctaaataa gggatttatt gatgctgtaa gaggctttga aaaacctgtt gagattttta
     6181 ttgagaatct tgataccaag cgcttcccgg agaacctgaa caggaaagag caaaaaatct
     6241 atatattatc ccgaattcag caaataaaac ctaatctcat tgccctaacc gacgatggag
     6301 cctttatctt aatggaatcg ctactaaaag agttttcagg gttagacaca attcctatag
     6361 tttttatggg tgtaaatcaa gaaaaggatt accctaaaaa ttttacagga gtactggaaa
     6421 caatcgatat aaaagcaaac ctggagttta taaagcagct tcaacctaat gtaaaaaggg
     6481 tttactgtgt aaccgatcaa acaatcacag gcaaggttat tggcgaaagg attaagagca
     6541 ccctacttaa gtataatcct ggttttgagg tgcaggttat tggaaactgc agttactatg
     6601 agttagtcga tactctttct aacctaaacg ataacgaagc aattctgttc ttacttttca
     6661 atagagatag caaggggaac tatttttcct atgaggcaat actggattca ttaagcagga
     6721 acgttaaagc tcccatttac ggtacatggt cgttttacct aaaccatgga atagttggag
     6781 gagccataat atcgggttac catcacggta aacgagccgg agagctagca agtctgctac
     6841 tgaaaggggt ttcgcccaaa actattgctc ctaccgatgg cccatccgat tttattgttg
     6901 accatttaca acttgtaaaa tattccattt cacaaaaact tttaccccaa ggaacgctca
     6961 tccttaataa aaataaaagg tttattcatt taagcagagg cacaactctg gttgttgcag
     7021 cgatagttct tttgctaatt gtgcttttaa ttttcatggg tgtgtatagc cgcatgaggc
     7081 acaaggcgct aaggttgcaa aagtactaca acactttgct ggagcaaaag aaccaggagg
     7141 ttgaaaacag cttaaagttt gcggaggagg caaatgagct aaagagtgct tttctggcca
     7201 atatgagcca tgaaatccgt acacccatga atgccattgt tggtttttca aagctgataa
     7261 aggaatcatc gctgctaagt ccggaagagg taaacggtta tcttgatatt attgcagaga
     7321 atgctcaaag gctaatgagg cttattaacg atatcattga tatttcaaag attgactcaa
     7381 agcagctttc catctataaa cgccctgttc agcttggtcc cctgtttgcc aactgctata
     7441 gggctgccga ggtagagctc gacagggtga acaaaaataa cattgccatt gaaatgtcgt
     7501 ttcgtcccac cgatgcagcc cttgaggtag taactgatcc cgataggctt caacaggtgg
     7561 taatgaacct gctaaacaat gccattaagt ttaccagtag tgggcggata gttttaggtt
     7621 acagtgttat acacgattta ctcagagtga gcgtggagga cacaggtatt ggtatcgact
     7681 cacagtatca cgatgcagtt tttgagcgtt ttaggcagat agacggaaca ctgaaccggg
     7741 agtacggcgg gtcaggttta ggcctgtcaa tatgcaaggg aatagttgag gctatgggag
     7801 gtaggattgg tgtggactca aggccagagg taggatcaac cttctggttc gaggtaccca
     7861 taagcttagc cacgcccggg cagcatgttg aggtaccagt taaggcttca aagtcaaagt
     7921 tgaaaaacaa ggttttgctc attgttgagg acaacgagcc ctcggcaatc cttttaaggg
     7981 agatgctcaa gggtacgggt gcagtgctcc ttcaggctgc tacaggcaaa gaggttgcaa
     8041 ctatcctgaa cgataaggtt aaaattgata ttgcgctggt tgatgtttac ctacccgata
     8101 tgtcgggcta cgaggtggtt acaaaaatta agcaggctca gcctaacgtt agggtaattg
     8161 cgcaaacagc taatgctatg gacaccgata gacaaaagtc gcttgaggca ggttgcgatg
     8221 agcacatcac aaagccaata gacaaggata aacttattga actactttca atgttacttt
     8281 cctgattttc ctaaaggaaa aacagtgcaa cgcctaccac tgtaattact gctcctacaa
     8341 gctctttagc agtaattttt tccttgaaaa caactacagc tggagcaata attaaaacgg
     8401 gtacaatcga catcaaggct gacgcaactc ctgttgaggt gtattttacg gctaaaagcg
     8461 aaaaggaaac acccaaaaac gggccaaaga atgcccctat taatagccgc ttcatagcct
     8521 taccgtttcg taatgcctcg gaaaggtttt tccacttatt tgaaaaggca aaaaagagag
     8581 taaagccaac aattcctgta ataactcgta tttgaacgga ggggataaca tcgtagccag
     8641 ccattccgta cttactcaaa actaagcctc cggcttgtcc cagcgcacca ccaaatgcta
     8701 aaaggatacc tccaacagga tacgaaaacc gaacccgttt cccatttccg ttctcatcgg
     8761 ccgattggcg ctccaatact accaatacta tacccgctag cacaacagcc atggcaatgg
     8821 cctgcttgag cgttagggtt tcgcccatgg ttatccaacc tatgattgca gccataggcg
     8881 gagctaacga cattataagc atggagatac gggagccaat aacaacgtac gactgaaaaa
     8941 gaaatagatc gcctaaaaca aatcctacca aacccgaaag cgatagccaa acccactgat
     9001 gcgtgccggc atcaaccgga aatgcttttc cgcgacttat cagggcatac accgaaagga
     9061 atataaaggc agtacccagc cttaaaaggt taaccgaaag cgaacccacc tttcggctgg
     9121 ccgattcaaa ggccagggcg gttacagtcc aaaaaaaggc tgttagcaaa gcggcaagct
     9181 cgccgtagta ttgttccata aattcacttt aataaatatt acccagttgg agtttaatgg
     9241 agctgtttgg tttaatactc cataaaataa attgggctgt cccttaagga acagcccatt
     9301 gtatcttgtt cattaacctg ttacagagcg ttaacgtgtt tctgaattga actcttaagg
     9361 ttagctgctt tattcttatg aatgatattg ttcttagcta acttatcgag catagccgat
     9421 actttaggaa gtaattccat ggctgcagcc ttatcggttg tgttgcgtaa agcctttaag
     9481 gcattacggg ttgtttttgc atagtaacgg ttgtgttccc tgcgagactc agtttgtctg
     9541 attctctttt cagctgattt atggtttgcc atcgctaaaa aagtattttc atgtttagta
     9601 gcccgtaggg gaatcgaacc cctgttacaa ggatgaaaac cttgcgtcct aacccctaga
     9661 cgaacgggcc attgtttact ttgcaaagag cattacctca tgaaagcggg tgcaaaggta
     9721 aacgataaat ttttttctgc aaaatatttg ctaaaatttt ttacttaaaa gtgtatcgaa
     9781 ctgaaaatcc ggcattaaac caaataccag gcgactcaat aatgttaaaa attggcagaa
     9841 tgtccagccc caagcaaatg ggagcatcct taaaagtata ctcaaggcca attacaccgt
     9901 cggcaccaag gaaaactttg ctaccgtatt cgctctcaaa tggattcttt ttatcggcat
     9961 tggcagtagc aaaatgggca ccaaatccgt agtaccactt taaacccggg gcatcgggta
    10021 taggattgtg aagctgataa agtccggtaa taccaattcc tccccaccca aacgacagaa
    10081 caccctcaac tgctgatttc tgggcaaaga aatgtttaac ggtaactccg gaggggtagc
    10141 cacctctgag gccaagacca gttttatagt cctgggcatt tgcagctgaa taagttacaa
    10201 acactgtagc gagaataaag agtaatcttt tcatattata ggtatttagt tagtgcaaat
    10261 atattcattt gacattaaaa agcgttagtg tttaacaacc tcatcagcta aatgttgtaa
    10321 atcggttcca gcaagattgc ctgaagtcat tatcaacagg ttgctattgc cccaattgtg
    10381 ctgtaatagg acgtcgacaa ggttgtttgc atcagtaaaa acagcaggat ggggatcacc
    10441 aaatccttga attacatcgt ttgggtcaat tggtggcaat tttttcaatt ccagggcatg
    10501 gggattgaag aaaacgtatg caacatcagc acccgtgaga gttccattat actggcctaa
    10561 aaaatctttt gtcaggctac taaaggtgtg tagctcaaag cataccacca gttttcgggc
    10621 aggatatagc tccctaacag cctcaatggt agctttaagt tttgatggcg aatgagcaaa
    10681 atccaaaaaa acactggtgc ttgttccttg cgctagcagc tgcaatcgct ttgaagctcc
    10741 ttcgaaagtt tcaatggcat tgtagaattg tttttcagta actccaaggg taatgcaagc
    10801 cgctttagca gcggatatgt tctgcatatt gtgctttccg aataccttta atgggatgct
    10861 tgtgccattg aaatcaaggc tccagatgcc gtttgctata ccatgtggat gggtatggta
    10921 gggaattctt ttgatatcgt tccgttggct ttcttctaca accctacaaa cctcagggtc
    10981 gtcggcacag tatatgattt ttccgttggg ctcaatacaa tcaataaact tagcaaactg
    11041 ttcccggtag atatcaaaag tgggaaaaac gttaatatga tcccatgaaa ttcccgatat
    11101 gattgcaatg tttggacggt aaacatgaaa ttttggtcga ggatcgatag gggatgtaag
    11161 gtactcatcg ccttcaaaaa cagcaaatgg cgcatcatcg gagaacgaca ccatattctc
    11221 aaagcccttt agctgagcgc caaccatata atcgaactta tagttgaggt gctttagcac
    11281 gtgcattatc attgatgtga tggtggtttt gccatggctt ccacccacaa caatccttgt
    11341 tttttgtcgc gattgctcgt aaaggaattc ggggtacgaa tagatcttta tggcaagttc
    11401 ttttgcccta agcaattccg gattatcggc tcgtgcatgc atacccagca caatggcttc
    11461 aatttcaggg gttataatgc ttgggttcca accctgatgg ctaggcaaaa gaccatggtt
    11521 cttaagacgt gaaagcgatg gttcaaaaat ttcatcgtcg gaaccggtta cctggtaacc
    11581 ttttagcttc atggctatgg ccaagttgtg cattgcgctg ccgcctatag caataaaatg
    11641 aactcgcatt tcagtttctg ggattgaggt tatggaaata tgttttctac cgtttcttat
    11701 tgttcaagta attcttccat tcaatggtat tgccgtaaaa cacgttaaaa tcaaccttac
    11761 cctgaactcc ttttagtcgc ccctcgtggt ggtactgcca aaaggtccat gacttctcaa
    11821 tttcgggggt atgatcgaaa gagcagatcc agatatcgtg atcgggaaac gtttcgctaa
    11881 tgtaccattt aaatgtatcc ttattggtgt aaattaaaac cttttgtcct gttgtttttt
    11941 caacaagttc aacaaagtca accaggtcgg caattatttg cttgcgtggc ctacgaacca
    12001 cattgcccca ttcctcaaca tcaaccacag ggggtaaatc gagttcaacc ccatcaattg
    12061 ccctcaagaa gttttgtgcc tgagcaatgc ccctccggtt gaagttaaag aaatggtaag
    12121 cacccacggg gatacccgct tcccttgcac gggtaacatt gttgcgaaac gatttgtcct
    12181 taagggtggc accctcgctt gcctttataa aggcaaaatc aaccttatga tcctttactt
    12241 tttcccagtc aatttttccg gtgtgagctg atacgtcgat accccaaatc aagtaacggt
    12301 gatgaggatc ggaaatgaat cgtacaataa tccatacggc taaagcacca ataaaaaggg
    12361 caacgggtaa aatgtactgg ttatttcttt ttttcttctt catagtatag ctgcaaatat
    12421 acctaaatgt tgtaaaaatg cagtgttagc taatcaatat caatttaatt aactatcagc
    12481 atacttttgg aattaggata aaaattgctg tatatttaaa aaaaatttgg tatgaagttt
    12541 cgcatagtta aggctatagc tttaaatatc cccttactac taatactttt tgttgctcaa
    12601 tcggggttta ttagtgctca ggacgatttg ctggaaggcg ttaagccttc atcgcttgag
    12661 gcacttgcaa aggttctgga acgtcccgac gactctgtga aagtggttac attaaagtta
    12721 ctatgctggg agaaccgaaa cagtgatccg gataaagcca tagagtatgg acaaatggcg
    12781 gtttcgcttg ccaaatcgtt aaaccttaaa atggaattgt ccgattctta caaccgctta
    12841 ggaatagctt ataggaacaa aggacgatac gctcaagccc tggagtgtta ctttaacgga
    12901 ttggaggttt cacgaacaca tggttttgac aagcacctag cttttcagta taacaacctt
    12961 gccgacctgt ataataggct tgggctgtac ggaagagctc ttgagtttgg caagcgtgcc
    13021 atggagtatg cccaaaaggt taacgatgat tatacccttg cctacattta caacatcatg
    13081 ggtaccattt acaagaataa gaatgagctt gattcggcgc taaattgttt cacgcattca
    13141 ctggagctac ggcagaaaat tggatacaat cagggtatag caacgtcgca tctcaacata
    13201 ggatcaatag agctactgag ggaacaatac agtaaaagtt tacaaagcat tcaaactgcc
    13261 attgatttat attcacccaa aaacgatttg caagggattg caattgcata ctactacaaa
    13321 ggtcaactat acaataaatt aggtaacttc aataatgcca tttactgttt tgatgaaagc
    13381 ttaaagtata acgagagttt taagaacctt actgtatcgc gcgattgcta taacggtttg
    13441 gcatatgcct atgctaaaaa gaaactattc gataaggcat actattttag tagcaaggca
    13501 accgagataa acgatagcat atcgcttagc cagtacgttg agcgcttaac ccaattaact
    13561 gaggctttaa agtacgagga aaccctgaac ctacaaaagg aaagggaaaa ggcacttgct
    13621 gaacgcttgg atttccagcg aaatattata aaactttaca ttgcaatcat aatccttctt
    13681 gccctgatag ttggtcttgt aatttatttc tacataaaaa gggcaaagga tatcagacta
    13741 ctacaggctc agcgcgatga aattaacatg cttaacagta ccaaggatag atttttctca
    13801 attcttgccc atgaccttaa aaatcagtta acctccattg taaccattgc gcaaatggta
    13861 aaggataaaa gtcttgccat tggcgacgat ggattgatag agctttcgag gcgtttatat
    13921 gccctaggct ttgccacaaa cgatttgctg gaaaatgttc ttagctggat taaatcgcag
    13981 ggtaagcagg ttatagcccg aaagagcaac attgatttga aagagtttat tgacagggta
    14041 atactgaccc aaaagcctgc agccgagatt aaaagtgttg ccataaacaa cagggttgaa
    14101 cccggcatta gcattcacac cgatgccgat atgttaagca cggtggttag gaatttgatt
    14161 tctaatgcca ttaagttttc tagttccggt ggaagggttg acgtgttcgc ttttcccaag
    14221 ggcaaccatg tggaaatttc cattaccgat tacggggtgg gtatgcccag ggaaacaacc
    14281 gataggcttt ttaataccct agaatcattg acacaacctg gcacaatgaa cgagcagggt
    14341 agtgggcttg gcttaataat ctgcaatcag ctggtaaccg atttaggcgg acagattaag
    14401 gtaaccagcg agccgcaacg ggggagcact tttacggtta gcttgcctca cgttagttaa
    14461 agtacttacc aaccttgttt atagccgtag ggagggcaaa caccactact cggtcgtagg
    14521 gctttatttc tgtatcgcct ttagcaataa aggcgttatc gccacgaaca acacccccaa
    14581 ctatggcatc cttagggaag ccaatatcct tgagcttacc cttggttata ggggcgcccg
    14641 gtttaacaat aaactcaag
//
LOCUS       DAOSAL010000067        14489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_2828_length_14489_cov_48.763544, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000067 DAOSAL010000000
VERSION     DAOSAL010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 14489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14489
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2828_length_14489_cov_48.763544"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="PK049_09095"
     CDS             complement(<1..845)
                     /locus_tag="PK049_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61755.1"
                     /translation="MAVPSKFKVFTNRLFGAFKDTQQVESKRSALQNEYNEFIEYTES
                     AELKRFRELTQWFKSGEHQRVKAELKKLTYKGSAEFNIEKEYLSLAKSKEVKAYLNKG
                     GEVTPTVSRYLELKSKVESAEFQNRKKYLLSKNKFEQSEVYAKLVEYQRLQNSDKIKW
                     FLSLERDSSKFNEIREWKLEFYDDFDSNAIDHQKWLTKFFWGDALLNKNYSFTTDKQN
                     YTDKNFEVKNSILRIVTRKEKSEGLGWDSKFGFVPKTYDYTSGVINTGHILRLKEGKV
                     EAKIRL"
     gene            complement(865..1245)
                     /locus_tag="PK049_09100"
     CDS             complement(865..1245)
                     /locus_tag="PK049_09100"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOK61756.1"
                     /translation="MDRVEIEPTMETPKVVLDKDKAIIEFAGNSLPEDVNSFYQPVLE
                     WIDKYIENPNEKTEINMKFDYYNTSSSKMILKILEKFREVHKKGFSVVVNWHYMEDDE
                     DMIEAGEDYAEHLKIPFKMIPIQQ"
     gene            complement(1356..2048)
                     /locus_tag="PK049_09105"
     CDS             complement(1356..2048)
                     /locus_tag="PK049_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOK61757.1"
                     /translation="MNQRKIRVMLAEDDENLGYLLKEYLQAKEYDVDLYKDGEKAYKG
                     FQNNYYDICILDVMMPLKDGFTLAKEIRMVNPSMPILFLTAKSMKEDVIEGFAIGADD
                     YMTKPFSIEELLMRLEAILRRTRKDANGAEQTLFQIGKYVFDATKQTLTYEDDVRKLT
                     TKESELLKYLCLNRNTLLDRNFALKTIWADDSYFNARSMDVYITKLRKYLANDPTIEI
                     INVRGKGFKMIY"
     gene            complement(2060..>2305)
                     /locus_tag="PK049_09110"
     CDS             complement(2060..>2305)
                     /locus_tag="PK049_09110"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOK61758.1"
                     /translation="VKDNGIGISRDNLKKIFDQFYRVPSGNIHNVKGFGLGLSYVKKI
                     VEAHGGKIWVESKLGEGSTFSFFMPWSGPAEQSYIPK"
     assembly_gap    2306..2405
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2406..3346)
                     /locus_tag="PK049_09115"
     CDS             complement(<2406..3346)
                     /locus_tag="PK049_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61759.1"
                     /translation="MRRKLLFILTGVITIASISLVLLQSKWIRIAVQIKEEQFAQTAR
                     LAMERIIDEVEKQETIVQVIDEIKPYYSVNTSGSAQMSYRQDILNKTKSGFRTKQISQ
                     QVFTLNNLDTLKLPAITKNLFDSSLLGNLPVKISSDPTVSNQQQLSVNVNDKLLKKTV
                     FVENIVDRMIRVELPLNERISQEQLDSIIHRELVRKGIDARYEYRVTNEKDSTIYSSP
                     RFKANFRGLVLRDKLFPNDFFARRYFLTLYFPNQKTYLLGSLGPMTFGTLLLTLLIIS
                     IFTITLYIIFKQKRISEIRNDFVSNMTHELKTPISTIS"
     gene            complement(3465..3872)
                     /locus_tag="PK049_09120"
     CDS             complement(3465..3872)
                     /locus_tag="PK049_09120"
                     /inference="COORDINATES: protein motif:HMM:NF033205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPExxxVDY family protein"
                     /protein_id="HOK61760.1"
                     /translation="MAASKKQILKDEPTEFKLLAIASSLSISQMVWNINQACTFSLTH
                     NTELEDILGFPSFTDRQTFTGRVITIIGNKVTGRVLHAKLSNIDFLMEISGDTDEQFI
                     ADTIKQLKSIKEIQVITQVQPSLLKLKEPYCAE"
     gene            complement(3939..4994)
                     /locus_tag="PK049_09125"
     CDS             complement(3939..4994)
                     /locus_tag="PK049_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009433443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOK61761.1"
                     /translation="MCKIILQRATIVFAFLLLSVHLLFAQATDQKEQYKVSCIAFYNL
                     ENLFDTIPGPNDTEFTPEGANKFTSARYWHKIDHMSDVISQIGGELFPGGPAILGVCE
                     IENRNVLEDLIASPKLKPSGYSIVHYDSPDARGVDVGMIYRPEFFKVTSHRSATLHVP
                     DNPNFKTRDQLVVSGLLDGEPLHIIVNHWPSRRGGEKRSLPFRMAAARLTRSIVDSIL
                     ATDANAKIIIMGDLNDDPDSPSIVKGLGANPYREKLKPGELFNAMGSLYHEGIGTLAY
                     RDSWNLFDNLIITQALLNGDKSTWTFFKSKVFNKPFLQQPSGQFAGYPWRTYVGNNFT
                     GGYSDHFPVYLFLIKKK"
     gene            5150..7867
                     /locus_tag="PK049_09130"
     CDS             5150..7867
                     /locus_tag="PK049_09130"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOK61762.1"
                     /translation="MRRLLLMLMVGLFYSTTTVLAQYNVSGYVKDSQTGSALPGVTVQ
                     VVGINQATTTDANGYYQFSGLQAGKYIIDYILSGYQTVENEVNVEGDLQLPDVTMNRG
                     LTDDAYATFNEVTVSLDDLESESKGQAVSGLLQSQNDAFSSAASFTFSAARFNIRGYE
                     PEYSLLFMNGVPVNDPESGFAGWSSWGGLNDVTRNRESRNGLTPTDWSFGGLGGATFL
                     NVRASQQRIGTRISYASSNRTYSNRLMFTHSTGLMENGVAFTISGSRRWAEEGYVEGT
                     NYDAWAYFIGIEKKFNEKHSVAFTTFNAPIKRAMQGVSTDEAKNLARTNFYNPNWGYQ
                     NGEKRNARVRFQQEPTFILNHYWNINSNLKLTSTAGFSFGTYQTTSLNWYDAQDPRPD
                     YYRKLPSYWVDSDPAVISQLADAFRNDPSVGQLDWDYMYQVNYNSKDANDSLRSKYVV
                     ENRITDSRQLTLSSVVNWSILPELKVNGGIDASIYKGKNYKRMDDLLGGQYWLDIDQF
                     VERDYFDNPNLAQNDLDNPNRKVKVGDTFGYSYDANVNNGNLWAVANYALNRIDIYAG
                     ANYSYSEFWRTGNMRNGRFPDNSKGDSPKQVFHNYGVKTGATWKITGRHYLDANLAYL
                     TRAPFFRNSYISPRTSNFVIPGLTSERIAAVDVNYNLRTPYIKARVSAYYTKFKDQTE
                     LKSFYDDTYRTFINYTMRGIEKVHRGVEVGAEIKLSTTLTLNTAASLGSYQFTSRPLA
                     TVTQDNLGTVLQQDQVVYIKNFYVPNTPQTAAMLGLRYASPKYWFLGAEVSYYDHSYI
                     DFASGKRTAEALQGLEPNDPRRDALTRQEKMPSAMLIDANIGKSWRYKSYFINLNFQV
                     TNILDKTDFKTGGFEQARYSITEQTSNKFPPKYFYGFGRTYFLILGLRF"
     gene            7888..8751
                     /locus_tag="PK049_09135"
     CDS             7888..8751
                     /locus_tag="PK049_09135"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5689 domain-containing protein"
                     /protein_id="HOK61763.1"
                     /translation="MRKVAFILTILTAGALIISAPGCVDFTYDEVEPRVDSTTLVANT
                     TIAELKAMYPGELYQLNENTFFQRDSIIIEGVVTSDDKEGNFYKSIFIQDATGAIELK
                     LNKTSLYNDYKRGQKIVVYCNGLYLGDYGGQIQLGSIYNNNGNWEISGLEGDPIIKMH
                     VFKKGKQLVDVAPLAMTPALLTQVNIGKLVKFDNVQIKDTLSPITDTTYTYADNINKV
                     TVNHTLVACSQTYPSTVVLRTSGYARFAGTTIATKNGTVTGILTYYKGTYQLLMRDLN
                     DINFTNDRCQQ"
     gene            8768..10819
                     /locus_tag="PK049_09140"
     CDS             8768..10819
                     /locus_tag="PK049_09140"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5689 domain-containing protein"
                     /protein_id="HOK61764.1"
                     /translation="MKRKYFIVLAILALAGLVLVTGSCVKEDFDTPPVYSKVATWEKS
                     ITIAQLKTLYNTAPALIKDVAPAGFWKNIYDENQKDSISGIFVDAYVLSSDSAGNFYE
                     TVTIADETGGIDLKINTSDIYATYGLKPGKRVLVKVDNLAIDNYNGMYQIGLGYTDGG
                     VLKVTGIDPSAVAKTIQVTGEKISLNPETITIPTLSTDKVQKLVKLEGVQFWNPAATY
                     SIPGVNTNRILVDQNGNQIILRNSGYAKFTKDQVPTGSGSITGVLSIFGSTYQLLIRD
                     TLDVKFNQPRFGSEVPAKNTTIEQLKLFYDGSPLYQIPYDLVIEGVVTANDESGNLYK
                     QLFIEDETGAIEFKVDVTGLYTDFPVGTKIRVKCKDLYMGTYGGVIQLGGNFGGQIGR
                     LAASDFYKNVFVISTGNEVSVTETSIAQLSNNLIGKVVTLSGVQFADYEMGKTYAESA
                     STTNRILEEVATGKTIIVRTSNYANFAGIELPTGRGKITAILTKYNSDFQLTIRKVGE
                     VRLIEPRLNKNFILNEDFTLATVNSPISINGWQTIAVAGTKVWNAKQYSGNVYARMSA
                     FQSNESSNIAWLISPKITIPSGKDVFFKFDTEYSFWANAILEVYISKNYDGTNPGTAE
                     WTKLDATIANQSVGQYSWVNSGLIKLNDFAGDAYIGFKYTGSNTLSTTFDVDNVSVYT
                     F"
     gene            complement(10887..12050)
                     /locus_tag="PK049_09145"
     CDS             complement(10887..12050)
                     /locus_tag="PK049_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOK61765.1"
                     /translation="MSKILVIDDEKAIRNTLKDILEVEGHSVDVAEDGHAGLEMFDNG
                     AYELVLCDIKMPQMDGIEVLEKLQERQPDVPVVMISGHGNIDTAVDAIKKGAYDFIEK
                     PLDLNRLLITIRNATDKTVLIQEAKTLKRKVSKAFDIVGQSPAIVKVKEMIDRVAPTE
                     ARVLITGPNGTGKELVARWLHEKSNRAAMPFVEVNCAAIPSELIESELFGHEKGAFTS
                     AIKQRKGKFEQADGGTLFLDEIGDMSLSAQAKVLRALQENKISRVGSDKDITVNVRVI
                     AATNKNLTQEIEKGNFREDLYHRLSVIIINVPPLCDRLEDIPLLAQHFNEQICAEYGI
                     PPKVINPDAIEELQKLKWTGNIRELRNVIERLIILCDKQITGNDVKAFAGPVF"
     gene            complement(12211..13128)
                     /locus_tag="PK049_09150"
     CDS             complement(12211..13128)
                     /locus_tag="PK049_09150"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61766.1"
                     /translation="MIIAIIDMGTNTFNLLVAQTSGKILHESKLPVKLGEGGFRSGSL
                     TPEAMQRAFKAINTHLITAKRLGANRCFAFATSAVRDASNGQEFVDEVKQRFNIDVEV
                     IDGNREAELIWKGVNAAIGLGKSPAVVVDIGGGSNETILANSEQIFWLQSFNLGVTRI
                     AEGFPISDPITTHELEAIESHMLQTVEPLLLAAANLNPRVMAGSSGAFDSFRKILAHK
                     GIIHDTSKFSAEIPVDEYLKLHRFFVTSTHAQRLALPGLDPIRVDLIIPASIFVNLLL
                     QKLGIKRMFQSDYALKEGFWVELSNKDKG"
     gene            complement(13125..13766)
                     /locus_tag="PK049_09155"
     CDS             complement(13125..13766)
                     /locus_tag="PK049_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOK61767.1"
                     /translation="MDQINIAELYRLVKDFFEHDTTGHDWWHVHRVTQLALYLARHEG
                     ADVRLVEPAALVHDTDDWKLSGANGYPNATRMLLAVGYNNHQVDRILEIVEQVSYKGA
                     GVDTAPSSLEAMVVQDADRLDAIGAIGIARAFAFGGSKHRPLYLPNVKPQLHSTFEEY
                     KTAKGHTINHFYEKLLLLKDRMNTATAKALAEERHRFMESFLEQFYREWNYKG"
     gene            complement(13849..14241)
                     /locus_tag="PK049_09160"
     CDS             complement(13849..14241)
                     /locus_tag="PK049_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61768.1"
                     /translation="MGIQYYGICKNCGRRFKIMDGGGFTFHMLRCDTCGKHKSVNFEQ
                     LGELHLRYLKGLNQPYSLATAHYDNLIRELFPGEPLPKDSYEAAIEEFVGKCRCGGQY
                     RFNAPPRCPRCRCTDFEEDPNGDAFLYD"
BASE COUNT         4089 a         3191 c         2995 g         4114 t
ORIGIN      
        1 aggcgaattt tagcctcaac tttcccttcc tttaacctta atatgtgacc agtatttata
       61 acgcccgaag tgtaatcgta agttttggga acaaagccaa actttgaatc ccaaccaagt
      121 ccctcagatt tctcctttcg ggtcacaatt cgtaaaatac tattctttac ctcaaagttc
      181 ttatcggtat agttctgctt atcggtggta aatgaatagt tcttgttaag gagcgcatcg
      241 ccccaaaaga actttgtaag ccatttttga tgatcaatgg cattggaatc aaaatcgtcg
      301 tagaattcaa gtttccattc tctaatctca ttgaacttgc tgctgtcgcg ctctaagcta
      361 agaaaccatt taattttatc ggaattttgt agcctttggt actcaactag cttagcgtaa
      421 acctcgcttt gttcaaactt attctttgat aaaaggtatt ttttgcgatt ttgaaattca
      481 gctgattcca ccttgctttt tagctcaaga taccttgaaa cggtaggtgt aacctcaccc
      541 cctttattca ggtaagcttt aacctccttg gatttagcta aggaaagata ctccttttca
      601 atattgaact ctgcggagcc cttatatgtt aactttttta gttcagcctt aaccctctga
      661 tgctcaccac tcttaaacca ctgggttagt tcgcggaagc gttttaactc agccgattcg
      721 gtgtattcaa taaactcatt gtactcgttc tgaagagcgc ttctttttga ctcaacctgt
      781 tgggtatcct taaatgcgcc aaaaaggcga ttggtaaaaa ctttaaattt cgagggaact
      841 gccattggtt taaggtttag ggtattactg ttgaattggt atcattttaa agggaatctt
      901 caggtgttca gcataatcct ctcccgcctc aatcatatcc tcatcgtcct ccatgtaatg
      961 ccagttcact actactgaaa aacccttttt atgaacttcg cggaattttt cgagaatctt
     1021 caggatcatc tttgatgagg atgtattgta gtagtcgaac ttcatgttaa tttcggtttt
     1081 ctcattagga ttttcaatgt acttgtctat ccactccaga accggctgat aaaatgagtt
     1141 aacatcctca ggcaaagagt ttcctgcaaa ttcaataatc gctttatctt tatcgagaac
     1201 aacctttggg gtttccatgg ttggctctat ctcaactcta tccatatcaa ttatggcttt
     1261 ttatacttaa taaccataaa gttattaaat atttatttaa aaccaaacct taccaataca
     1321 aaaaaacgaa aaaaagttga atatattgac tgcagctagt aaatcatttt aaacccttta
     1381 ccacgaacgt taataatttc aatggttgga tcgttggcaa ggtatttacg taactttgta
     1441 atataaacat ccatgctccg agcgttaaag tagctatcgt cggcccaaat ggttttaagg
     1501 gcaaagttac ggtcaagcag ggtgttacgg tttaggcaaa gatacttgag taattcgctt
     1561 tccttggtag taagtttacg tacatcatcc tcgtaggtaa gggtttgctt ggtggcatcg
     1621 aaaacgtact tgccaatctg gaaaagggtt tgctctgccc cgttggcatc cttgcgtgta
     1681 cgacgtagga tagcctcaag cctcatgagc agctcctcaa tgctaaaggg tttggtcatg
     1741 taatcgtccg cgccaatggc aaatccttca attacatcct ccttcatgga tttggcagta
     1801 aggaaaagaa tgggcatgct ggggtttacc attctgattt ctttggctag ggtaaaacca
     1861 tcttttaaag gcatcatcac atcaaggatg cagatgtcgt agtagttgtt ctggaagccc
     1921 ttgtaagcct tttcgccatc cttatacagg tcaacatcgt attctttagc ttgaagatac
     1981 tccttgagta ggtatccgag gttttcgtcg tcctcagcaa gcattactct aatttttctt
     2041 tggttcatgg ttatactagt tatttaggta tgtacgattg ttctgccggt ccgctccagg
     2101 gcatgaaaaa cgaaaaagta ctcccctcgc ccagcttgct ctcaacccat atttttccgc
     2161 catgcgcctc cactatcttt ttaacgtagc taaggcctaa gccaaagccc ttcacgttat
     2221 gtatattgcc gcttggaacc ctgtaaaact ggtcaaaaat ctttttcagg ttgtcgcggc
     2281 taatgccaat accattatcc ttaacnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnngatat ggttgatatg ggtgttttta gctcgtgggt catgttgctt acaaagtcat
     2461 ttctgatttc cgatatcctt ttctgcttga aaataatgta aagggtgatt gtaaatatag
     2521 aaataattaa caaggtgagc aatagggttc cgaatgtcat ggggcccaat gatcctaaca
     2581 ggtaagtttt ttggttgggg aagtaaaggg ttaaaaagta acggcgtgca aagaaatcgt
     2641 tagggaaaag cttgtcgcgc aggacaagac ccctgaagtt ggctttaaac cgaggactac
     2701 tgtatatggt actatccttc tcgttggtta ctcggtactc gtaccgggca tcaatgccct
     2761 tgcggacaag ttcgcggtga ataatggaat cgagttgttc ctgcgaaatc ctttcgttaa
     2821 gtggcagctc aactcggatc atacggtcaa caatgttttc aacaaaaaca gttttcttga
     2881 gcagcttgtc gttgacgtta acgctaagct gttgctgatt cgatactgtt ggatccgacg
     2941 aaatcttaac cggtaagtta cccagtaagc tcgaatcaaa aaggtttttg gttattgctg
     3001 gcagcttaag ggtatcgagg ttgttcagcg taaaaacctg ttggcttatt tgcttggttc
     3061 tgaatccgct tttggtttta ttaaggatat cctggcggta cgacatttgg gctgaaccgc
     3121 tggtgtttac cgagtagtag ggttttatct catcaatcac ctggactatg gtttcctgct
     3181 tttcaacctc atcaattata cgttccattg ccagtctggc tgtttgggca aactgctcct
     3241 ccttgatttg cacagcaatc cttatccact tggactgtag tagaaccaag cttattgatg
     3301 caatggtgat aacccctgta agtatgaata gtaatttcct gcgcataaga gcaaagttag
     3361 tttttatgaa atggcgcgga caactcttaa caaagtttaa ccctaagggg tgttttaata
     3421 acaagattat aaaggttgct gattatgaag cggtactgat ttaactattc ggcgcagtag
     3481 ggttccttta gttttaggag tgagggttgc acctgggtta taacctgaat ttcttttatt
     3541 gatttcaact gcttaatggt atcggcaata aactgttcgt cggtatcgcc gcttatctcc
     3601 attaaaaaat caatgtttga tagctttgca tgaagaaccc tacctgtaac cttattgcca
     3661 ataatggtaa ttacccttcc tgtaaaggtt tgcctatcgg taaacgaagg aaaacctaaa
     3721 atatcctcaa gctcagtgtt atgggttagt gagaatgtgc acgcctgatt gatattccac
     3781 accatttggc taatactcaa cgatgaggca atagccagga gtttgaattc ggttggctca
     3841 tccttaagta tttgcttttt tgatgcggcc atggaataat gggtttgctc acaaagttaa
     3901 agtttttgaa taaaaaaacg ggcgcttgcc cgtttatgct actttttctt tatcagaaat
     3961 aaatatacag ggaaatggtc ggaataccca ccggtaaaat tgttcccaac ataggttcgc
     4021 caggggtaac cggcaaattg accgcttggc tgctgcaaaa atggtttgtt aaatactttt
     4081 gacttgaaga atgtccacgt agacttatcg ccgtttaaaa gagcttgggt aataatgagg
     4141 ttatcaaaaa gattccatga atcgcggtag gccaatgttc caatcccttc atggtataag
     4201 ctgcccatgg cattgaatag ttcgcccggc ttcaactttt cacggtaagg atttgctccc
     4261 agccccttta caatgctggg actatcgggg tcatcgttca ggtcgcccat aataataatc
     4321 ttggcattag catcggttgc cagaatggaa tcgacaatgg aacgggttag cctggcagca
     4381 gccatacgga aaggtaggct acgcttttcg ccaccccggc gcgaaggcca atggttaaca
     4441 atgatatgca ggggttctcc atcaagcaaa ccgctaacaa ccagctgatc gcgagtttta
     4501 aaattagggt tatcgggtac atgaagtgtg gcagaacggt gcgaggtaac cttaaaaaac
     4561 tcggggcggt aaatcatacc aacatctact ccacgggcat cgggcgaatc gtaatgcaca
     4621 attgaatacc ctgaaggttt aagctttggg gaagcaatta aatcttcaag tacgtttcta
     4681 ttttcaatct cacaaactcc aagtattgca ggtcccccgg gaaataattc cccgccaatt
     4741 tgcgaaatta catctgacat gtggtcaatc ttatgccagt accgggccga cgtgaactta
     4801 tttgctccct caggggtaaa ctcagtatcg tttgggcctg gtatggtatc gaacaggttc
     4861 tccaaattgt aaaacgctat gcagcttacc ttatactgtt ctttttggtc agtggcctga
     4921 gcaaatagca gatgaacaga aagtagcaaa aaagcaaaca ctattgtggc cctttgtagt
     4981 attattttac acatatactg ttaatttata gaaaaagtat gccgacaaag ataaaactaa
     5041 acgaaaaaaa agaatgtaaa aaatgaaaaa ttatattaaa aagtgtcatt tttgcaagtg
     5101 caattatagg tttaaatttt agaattatcc actgaaactg aatatttaaa tgaggcgatt
     5161 acttttaatg ttaatggttg ggttgtttta cagcacaacc acagtactgg ctcaatacaa
     5221 tgtgagcggg tacgttaagg actcccaaac cggtagtgct ttgcccggtg taaccgtgca
     5281 agtggtggga ataaaccaag ccacaaccac cgatgctaac ggctactatc agttctcggg
     5341 tcttcaggca ggcaagtaca tcattgacta catcctatcc gggtaccaaa cggttgaaaa
     5401 tgaagtaaat gttgaaggcg atcttcaact gcccgatgtt acaatgaata gaggtttaac
     5461 cgacgatgct tacgcaacgt ttaatgaggt gacagtttcg ctcgatgacc tcgagagcga
     5521 atcgaaaggg caagccgtga gcgggttact tcaatcgcaa aacgatgcgt tctcctcagc
     5581 tgcttcgttt acttttagcg cagcccgctt caatattagg gggtacgaac cggaatactc
     5641 gctactgttc atgaacggtg ttcctgttaa cgatcctgag agcggttttg ccggatggag
     5701 ttcgtgggga ggtttaaacg atgtgacccg aaacagggaa tcgagaaatg ggttaacccc
     5761 aaccgattgg tcgttcggcg ggcttggcgg tgcaaccttc ctgaatgtac gggcatcgca
     5821 gcaacgtatt gggacacgca tttcttacgc atcgagcaat agaacttact ccaacaggct
     5881 catgtttacc cattcaactg gattaatgga gaatggggtt gctttcacca taagcggttc
     5941 ccgccgttgg gctgaagagg gctatgttga aggcactaac tacgatgcat gggcttactt
     6001 tatcggcatt gaaaagaaat ttaacgaaaa gcactccgtt gcgtttacta cctttaatgc
     6061 accaataaaa agggccatgc agggtgtatc gaccgatgag gctaaaaacc ttgccagaac
     6121 caacttttac aatcccaact ggggttacca gaacggcgaa aaacgcaacg ctcgtgttag
     6181 gttccagcaa gaacctacct ttattcttaa ccactactgg aatattaact caaacctaaa
     6241 gttgacctct acagctggat tctcgtttgg tacataccaa acaaccagcc ttaactggta
     6301 cgatgctcag gatccccgcc ccgattacta ccgtaaactc cccagctact gggttgattc
     6361 cgaccctgca gttataagcc agttagccga tgctttcagg aacgacccat cggttggaca
     6421 gcttgattgg gattacatgt accaagtaaa ctataacagc aaggatgcaa atgatagcct
     6481 ccgctcaaag tacgttgttg aaaacagaat taccgactcc cggcagctta ctttatcgag
     6541 cgtggtaaac tggagcatcc tcccggaact taaggttaat ggcggaatcg atgcgtcaat
     6601 ctataaggga aagaactata aacgaatgga cgatttgctg ggtggccagt actggttgga
     6661 cattgaccag tttgttgagc gcgattactt tgataacccc aaccttgctc agaacgactt
     6721 ggataatccg aaccgtaagg ttaaggttgg cgataccttt ggttacagct acgacgccaa
     6781 cgtaaacaat ggaaacctat gggcagtggc aaactacgcc ctaaaccgta tcgatattta
     6841 tgccggagca aactacagct atagtgagtt ttggcgtact gggaacatgc ggaatggccg
     6901 cttccccgat aactccaagg gcgactcgcc aaagcaagtg tttcataact atggggtaaa
     6961 aaccggtgct acctggaaaa ttaccggtcg ccactacctc gatgctaacc ttgcctacct
     7021 tacccgggct ccgtttttcc gcaattcata catctcgcca cgtaccagta actttgtcat
     7081 tccaggctta accagcgaac gaattgccgc tgttgatgta aattacaacc ttcgtacccc
     7141 ctacatcaaa gctagggtat cggcatacta cacaaagttc aaggatcaaa ccgaacttaa
     7201 atcgttttac gacgatacct atcgcacctt tataaactac accatgcgtg ggatagaaaa
     7261 agtacacagg ggtgttgaag ttggagccga aattaaactt tccaccacat taacacttaa
     7321 taccgctgcc tcattgggtt cataccagtt tacctcaagg cctctggcaa cggttacaca
     7381 ggataacctg ggaacagtac tgcaacagga tcaggttgtt tatatcaaaa atttctacgt
     7441 gcctaacacg cctcaaactg cagccatgtt aggtttaagg tatgcatcgc ccaagtactg
     7501 gtttttagga gctgaggtta gctactacga tcattcctat attgattttg cctctggcaa
     7561 gcgtaccgca gaagccttac agggacttga gcccaacgac ccccgtcgcg atgcgctaac
     7621 ccgacaggaa aaaatgcctt cggccatgct tattgatgct aacattggaa aatcgtggag
     7681 gtataaatcg tactttatca acctgaactt ccaggttaca aatattcttg ataaaaccga
     7741 tttcaaaacc ggagggtttg agcaggctcg ttactcaatt accgagcaaa cttccaataa
     7801 atttcctccc aagtatttct acggctttgg tcgtacttac ttcctgattt taggtctcag
     7861 attttaaaac gaattaattt ggttaagatg agaaaagtag cattcatact cacaatccta
     7921 acggcaggag ccctaataat atcagctccc ggatgcgtgg atttcacata cgatgaggtt
     7981 gaacccaggg tggattcaac aacgcttgtt gccaatacta ccattgctga gctaaaggcc
     8041 atgtaccctg gtgaactgta ccagctaaac gaaaacactt tcttccagcg cgattcaatt
     8101 atcattgaag gtgttgttac ctccgacgat aaggaaggca atttctacaa gagcatcttc
     8161 attcaggatg caacaggtgc aattgagcta aagctcaata aaacatcact atataacgat
     8221 tacaaacgcg gtcaaaaaat tgtggtatac tgcaatgggc tttaccttgg cgattatggt
     8281 gggcaaatac aacttggttc catatacaat aataatggaa actgggaaat tagcgggttg
     8341 gaaggtgatc caataattaa aatgcacgta ttcaaaaagg gtaaacaact tgttgatgtt
     8401 gccccattag ccatgacccc tgccttgctc acgcaggtta atattggcaa attggtaaag
     8461 tttgataatg tgcaaataaa ggataccctt tcgccaatta ccgatacaac ctacacttat
     8521 gccgataaca ttaacaaggt tacggttaac cataccctag ttgcctgttc acaaacgtac
     8581 ccaagcacag ttgttctacg cactagtggt tacgcacgct ttgcaggtac aacaattgcc
     8641 accaaaaatg gaacagttac aggtattctt acctactata aaggaaccta ccagctgcta
     8701 atgcgcgatt tgaacgatat caattttaca aacgataggt gtcaacagta aaagggagaa
     8761 gatgtatatg aaaaggaaat attttatagt acttgccata ctggcccttg ctgggttagt
     8821 attagtaact ggctcctgtg taaaggaaga ttttgataca cctcccgttt acagcaaggt
     8881 tgccacctgg gaaaagagta ttaccattgc tcagctaaag acactttaca atacagcacc
     8941 agcgctaatt aaagatgttg ctccggctgg attctggaag aacatttacg atgaaaacca
     9001 gaaagattcc atttcaggaa tatttgttga tgcctatgtg ctctcgagcg attcagccgg
     9061 gaacttttat gaaaccgtaa ccatagccga tgaaaccgga ggtattgacc taaagataaa
     9121 cacaagtgac atttacgcta cctacggact aaaacctggt aaaagagtgc ttgttaaagt
     9181 tgataatctg gctattgaca actataacgg tatgtaccaa ataggtttgg gctataccga
     9241 tggtggtgta ctcaaggtta cgggtatcga cccctcggca gtagccaaaa ccattcaggt
     9301 aaccggcgaa aaaatcagct taaatccaga aacaatcacc atcccaactt taagcactga
     9361 taaggtgcaa aaacttgtta agcttgaggg tgttcaattc tggaaccctg ccgcaaccta
     9421 tagtattccc ggagtgaata caaaccgtat acttgttgac cagaacggta accaaatcat
     9481 tttgcgaaat agcggttacg caaagtttac taaggatcaa gttccaaccg gtagtggttc
     9541 cataaccggt gttctgagca tatttggtag cacctaccag ctccttattc gcgacactct
     9601 tgatgttaag tttaaccagc cccgatttgg ttcagaggtg ccagctaaaa acactaccat
     9661 agaacagctt aaactcttct atgatgggtc accgttatac caaataccat atgatttagt
     9721 tattgagggt gttgtaacgg caaacgatga gtcaggaaac ctttacaaac agctctttat
     9781 tgaagatgaa acgggtgcca ttgagtttaa ggttgatgta actggtttat ataccgattt
     9841 ccctgtgggc actaaaattc gggtaaaatg caaggacctt tacatgggca cctacggtgg
     9901 tgttatacaa cttggcggta attttggtgg ccaaatagga aggcttgcag caagcgattt
     9961 ctacaaaaat gtttttgtaa ttagtaccgg caacgaggtt agcgtaactg aaacctcaat
    10021 tgcacagcta agcaacaatt taattggcaa ggttgttacc ctgtcaggtg ttcaatttgc
    10081 cgattacgag atgggtaaaa cctacgctga gagcgcctca acaacaaata gaatacttga
    10141 agaggtggca accggtaaaa ccattattgt tagaacaagt aactatgcaa actttgctgg
    10201 gattgaactg ccaactggcc gtggtaaaat cacagctatt cttacaaagt ataacagcga
    10261 ttttcagctt accatccgta aagttggcga ggttcgctta attgaaccca gactaaacaa
    10321 aaatttcatt ttgaatgagg atttcacact ggctacggta aatagcccaa tcagcattaa
    10381 cggttggcaa accattgctg tagctggaac aaaggtttgg aatgccaagc aatatagtgg
    10441 aaatgtatat gcacgaatga gtgctttcca gtcaaacgaa agtagtaata ttgcctggtt
    10501 aatttcacca aaaattacca ttccaagcgg gaaagacgtt ttctttaaat ttgataccga
    10561 gtattccttc tgggctaatg ctatccttga ggtatacata tccaagaact atgacggaac
    10621 taaccctggg actgcagaat ggacaaagct cgatgcaaca attgcaaatc aatcggttgg
    10681 ccagtactca tgggtaaatt caggattgat aaagcttaat gattttgcag gtgatgccta
    10741 tattggtttt aaatacacag gtagtaatac gctttcaaca acctttgatg ttgataacgt
    10801 atcagtttac accttttagt aagcattaaa aagataggaa aggccgggga aacccggcct
    10861 ttcctattta acctactccc attaccttaa aacactggcc cggcaaaggc cttaacatcg
    10921 tttccggtta tttgcttatc gcaaagtatg atcaaacgct caataacgtt tcgcaactca
    10981 cgaatattcc cagtccattt gagcttctgg agctcttcaa tggcatcggg gttaataacc
    11041 ttgggtggga taccatactc ggcgcaaatc tgctcgttga agtgttgagc aaggaggggt
    11101 atatcctcaa gccggtcgca aaggggcggc acattaataa ttataacgct aaggcggtgg
    11161 tataaatcct cgcgaaagtt tcccttttca atttcctgag taaggttttt attggttgca
    11221 gcaattacac gcacatttac agtaatatcc ttatcgctgc ctaccctgct aatcttattc
    11281 tcctgcaaag ccctaagcac ttttgcctgg gcgctaaggc tcatatcacc aatttcatcg
    11341 aggaataggg taccaccgtc ggcctgctca aacttaccct tccgctgctt tatggccgag
    11401 gtgaatgcgc ctttctcatg tccgaaaagt tcgctctcaa tcaattccga agggattgca
    11461 gcacagttaa cttccacaaa cggcatggct gcccggttac tcttctcgtg taaccagcgg
    11521 gctaccagtt ccttaccagt tccattgggg ccagttatta gaacacgagc ctcggtgggt
    11581 gccacgcggt caatcatctc cttaaccttt actattgcgg gcgattgccc tacaatatcg
    11641 aaagccttgg aaacctttcg cttaagcgtc ttggcctcct gaatcagaac tgttttatcg
    11701 gttgcattcc gaatggtaat tagcaagcgg ttaagatcga gaggtttttc aatgaaatcg
    11761 tatgccccct ttttgattgc atcaacagcc gtgtcgatat taccatgtcc ggaaatcatt
    11821 accacgggta catcgggctg acgctcctgt aacttctcaa gcacctctat tccatccatc
    11881 tgcggcattt tgatatcgca aagcaccaac tcataggcac cattatcaaa catctcaagt
    11941 ccggcatgac cgtcctcggc aacatcaaca ctatgccctt caacctccag gatatctttc
    12001 agtgtattac gaatagcctt ttcgtcgtca attaccaata ttttggacat aaccagtatt
    12061 ttggtgtaaa gataaaaaaa atagttagat gttaccagct aacagttcca gccccaaaac
    12121 cccaaaaaaa ctgctcgttt caatccaata attcgtaatt tcagaacggg tagttgaaac
    12181 ctttatcctt tatcctatat cctttattct ttacccttta tccttattac taagttccac
    12241 ccaaaatcct tccttcaggg cgtaatcgga ctggaacatt cttttgatgc caagcttttg
    12301 taacagtaag tttacaaata tcgatgctgg aataatcaaa tcgaccctta tggggtcaag
    12361 ccctggcagt gcaagccttt gggcgtgggt tgacgtaaca aaaaagcgat gtagcttcaa
    12421 gtattcatca acaggtattt cggccgaaaa ctttgatgtg tcatgtataa tccctttgtg
    12481 cgctagtatt tttctgaaag aatcgaacgc gcctgaacta ccagccataa ccctaggatt
    12541 taggtttgct gccgccagta acaatggttc aactgtttga agcatgtggc tttcaatggc
    12601 ttcaagctca tgggtagtaa tgggatcgga aattgggaaa ccctcggcaa ttcgggtaac
    12661 gccaaggtta aagctttgca accagaagat ttgttccgag ttggctaaaa tggtctcgtt
    12721 gctgccgcct ccgatgtcaa ccacaactgc tggactttta cctaaaccta tagctgcatt
    12781 tacccccttc catatcagct cagcctcccg attaccatca ataacctcaa catcgatgtt
    12841 gaacctttgc ttaacctcat ctacaaactc ttgaccattc gatgcatcgc gcacagccga
    12901 cgtggcaaat gcgaagcaac ggttagcgcc taagcgtttt gcagtaatca ggtgtgtgtt
    12961 tatagcttta aaagcccttt gcatggcttc gggtgtaaga ctaccgctcc taaaaccgcc
    13021 ctcgccaagc tttacaggta acttgctctc atgaagaatt tttccagagg tttgagccac
    13081 caataagtta aaggtattgg tgcccatatc gataatagct ataatcatcc tttatagttc
    13141 cactccctgt agaattgctc taaaaaactt tccataaacc tatgcctctc ctcggccaag
    13201 gctttagccg tagcggtgtt catcctatcc ttcaggagta aaagcttttc gtagaaatgg
    13261 ttaatggtgt gccctttggc cgttttatac tcctcaaagg ttgaatgtag ctgaggtttc
    13321 acattgggaa ggtacaaggg acggtgcttt gaacccccaa aggcgaaagc ccgggcaatg
    13381 ccaattgcgc caatggcatc gagcctatcg gcatcctgaa caaccatggc ctccaggctg
    13441 ctaggtgcag tatcgacccc agcccctttg tacgaaactt gctccactat ttccaaaatc
    13501 ctatcaacct gatgattgtt gtacccaaca gctaagagca ttcgggtggc attgggatat
    13561 ccattggccc ccgaaagttt ccagtcgtcg gtatcgtgaa caagcgctgc tggctcaaca
    13621 agccgaacat cggccccttc gtgcctggcc aaatataagg ctaactgtgt tactctatgc
    13681 acatgccacc agtcgtgccc ggtagtatcg tgctcaaaaa aatcctttac cagcctgtaa
    13741 agctcggcta tattgatttg atccatccca attctttgcc gctaatatcg tttttttata
    13801 cgagactaag aactaatttg aagcgcctat atctattttc aaaatttatt agtcgtaaag
    13861 aaatgcatcc ccattaggat cttcctcaaa atcggtgcaa cgacaacgtg ggcagcgtgg
    13921 gggcgcatta aacctatact gaccgccaca cctacatttg cccacaaatt cctcaatggc
    13981 agcctcatag ctatccttcg gcagaggctc acccggaaaa agttccctga ttaaattatc
    14041 gtagtgtgcg gtagcaaggc tatatggctg attcaaacct ttaagatacc ttaaatgtaa
    14101 ttcgcccaac tgctcaaagt ttacagactt atgttttccg catgtatcgc agcggagcat
    14161 gtgaaaagta aatccaccgc catccattat tttaaaacgt cggccgcaat ttttacaaat
    14221 accataatac tgtataccca tagcttttaa aattttattt tgatgacaaa tatgaaatgt
    14281 tgaaacgaca aattctgtcg gtattaaaat tagtggaaat ttttgccatt tggctactcc
    14341 cacccctcaa gtccatcgaa aagagttggg ccagattgaa tgtttttgcg cttgttgctc
    14401 ttttttctgt agttaaggaa gaaaaatcgc tgggcatcaa aagttgaaat tgcctttctt
    14461 accctgttta ccgtttcctt gctcacctc
//
LOCUS       DAOSAL010000068        14403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_2850_length_14403_cov_45.523906, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000068 DAOSAL010000000
VERSION     DAOSAL010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 14403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14403
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2850_length_14403_cov_45.523906"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(506..3313)
                     /locus_tag="PK049_09165"
     CDS             complement(506..3313)
                     /locus_tag="PK049_09165"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOK61769.1"
                     /translation="MKPVEGSNWHILLYSSNQSNCNEWVKELLNRPEFSHCKFLQVDS
                     LPDLNNVLSQRFNGVLLVHLTSNSYGLIKQAVSVRNDISPQVQVVALLDHDALNLDSA
                     KLLSTDIDDVVHCTSKEWLQATLLKYYFTSKADSCILPYAPFDRYSTHVDRIHSEFPF
                     GIVRVILKPDVYVEYINREAERILGFNLKQHKAQPELRNRFIPDERIKLILENAGTSN
                     EFIKRRFKISVGNDNYKWVEAIYIPGFKNNQSFQSIEVILRDVTREEIDKALQKAVYD
                     IARMTSTNFSLNRIFTVTHKIVSDLINIDNFYIALYNSENNEVSFPYFVDSTDIKTPN
                     PRRDGHGLTEYVIRTGKTLLCQPSCFDELIFKNEIDLIGTKPMAWLGVPLKFHGNILG
                     VMAVQHYTNINAFGESEQYILEYFSEQVARTIDYKRKADTVKLLSTAVEQSPVSVVIT
                     TLKPEIVYVNSTFTKVTGYEPHEVMGKNPRILQSGQTSKELYVELWDTLTSGSTWKGE
                     FINKRKNGEVFYEESIIVPITDELGKNAYYLSVKIDITDKRRLINELIIAKEKAEESD
                     KLKTAFLQNISHEIRTPMNAIVGFAEILESDHDDEDKISYYTGVIKQRSYDLLDIVNE
                     LLDISRIESGQLKAEFKPFDLNGIFTDLVQTFEGYKKRMEREEIELRLLPLPAHVSNL
                     IESDEPKLRQVLVNLIHNALKFTHSGYIEFGFHELTNGSIVFRVADTGIGIPHDMQDK
                     IFERFQKVSPKGVIYDGLGLGLTIVQSLANLLNGKIWLESEVGKGTTFYFSIPYKPIK
                     KVEEIEIEPVNPEVIKNLNLLVVEDDAFNIAYFNELFSGLSVNYHIENTGSSAIDHFE
                     QNSSYDVVLMDIKLPDMTGYEVIEEFKRINPTVPIIAQTAYAAESDRKKAFEVGCVDY
                     IAKPIFRDKLIGMLQKHLVKG"
     gene            complement(3332..3709)
                     /locus_tag="PK049_09170"
     CDS             complement(3332..3709)
                     /locus_tag="PK049_09170"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOK61770.1"
                     /translation="MENIKDLSNKTILVVEDDKFTSLIIQNFLAKTKAKIVTAYNGEQ
                     AVEQARAHNPDLIFMDLKLPGISGHEATRRIKEFLPNTIIIAQTAFVTDADRAEALDS
                     GCSDFLTKPLSSDKIYRTLYKFL"
     gene            complement(3714..4982)
                     /locus_tag="PK049_09175"
     CDS             complement(3714..4982)
                     /locus_tag="PK049_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61771.1"
                     /translation="MRVYISILSCTLFFGFAVTGQNLIRNGSFEKYYVCPDNYTVEYT
                     KRFLPNWVMPTKGTPDYFNRCSKEMVGVPQNFMGSIFPAEGDGFIGLVLLDTPDVKEE
                     IDYRDYVHSGPLAPVNITNANIKKPDTKRKVKPINYREYIQTQLVTPLQPNQLYRISF
                     KYALAQHSTFVSNRLGIAFTESPITQKDGVLPIKPAVIIDTAVLYCTPGIWVEFADTF
                     RTQGGEFFMTIGNFFPDSQSTYFPNDISSLNTTLQRAILTNQVAYYYLDNIRLEPVKK
                     EDVKQLSPRFIPFSMLKRHELSKIDTLCRYYALLDEVYFDIGQPGTKPRSLCQLYWLT
                     DFMTQNPNLGIELNGILHEIENDTVGASSRLKAMMEYLQEMGISGGRIRLRIFEDNKA
                     ISPKYRFYREGLPCVFYSSLVAVRFFTIKE"
     gene            complement(4991..5455)
                     /locus_tag="PK049_09180"
     CDS             complement(4991..5455)
                     /locus_tag="PK049_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61772.1"
                     /translation="MFKQIILSASLFIAAIVTVQAQCTGFHTIRECTPPDISDFKRFG
                     QSRSRMVEAKKTFTYQVVLFGGYDYKIGICTEKGYYPVQFRIINAEDNSVFYDNANDD
                     YVETVGFTVEKTKNVLFEITVLASKKEFKDATDSRVCAGVAIYWRKVPKTGF"
     gene            complement(5473..6363)
                     /locus_tag="PK049_09185"
     CDS             complement(5473..6363)
                     /locus_tag="PK049_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61773.1"
                     /translation="MKQYRFYFVVLAMLFAAMFYSCSGGKTNQADVNTELSEDTLVQD
                     QMEIMLPTPSEVLSIVLTSGIKYSDSYMAPLGIEKKAVMVKHKALILGVYFADFAYTN
                     YYNQRNASSEYFKALQELTRDLGIGSILNDAYFKRFDANLNNPDSLDAIFEDFSQNAY
                     NTILESGNRELLSMIGIGAAIEVLQIGINSIENAANPDEAIATVIDQSAVFDNYYNNF
                     IAYNSSKPEFKSLTEDVTEIFKFFKEKLSSAGNESVVTDSKEHFTITGGDTTKLSKAD
                     LAQLRNMVANARKKLIDLKY"
     assembly_gap    6476..6575
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6576..7751)
                     /locus_tag="PK049_09190"
     CDS             complement(<6576..7751)
                     /locus_tag="PK049_09190"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOK61774.1"
                     /translation="MNRGGYTYIFYENYNIPIFFLTSIIASMFLGMIVTSDSVIKDAN
                     INKREAFLFLNRKAYYNSKVLFYFGLTILQTLIYTAISIYILKVKGMFLPIWVILILM
                     GFFGNIAGLIVSTIFKSLSAAYLIVPFLVIPQIILSGITIPFDRMNHRLANPEVVPVI
                     GIVSPSMWGMEALLVHQFKNNAYERHFFEIDMAESQSRIQSQYLIPKLFQLIQSYKQT
                     DSVNRLRLKHLIKSGLGQLNIDIAEFPQPNTAEFRKLEIVLHELQQKQQQNYSRIIRK
                     KDQITTKLQSQFYSAEAFLEFKQMHTNRGVDDLTLARKNITAIQVVENRLVQTIDPVF
                     KIPTNRWGRAHFLAPAKRIANQLFDTFTFNVVVLLVFLVSIYLILLFDILPKLSNSMV
                     "
     gene            complement(7708..9546)
                     /locus_tag="PK049_09195"
     CDS             complement(7708..9546)
                     /locus_tag="PK049_09195"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOK61775.1"
                     /translation="MNEFLVNSQVNLFAHIVLNGDASLQRLAEIYTEVLFTRLVNPRI
                     GKKQKEELKQVVRNSKQANSSIDVKKICSKLNSESSIQQRLILLLNLLEFGLFVQKNA
                     ILLSKSNSIAGKIKQIADELKISAETYSLCSSFVSGKLYEVHDRRNILIVKSVSPELE
                     GFHFVKREQIDGYFVFVYFPDVSTIFFRYQGKENLQLNSKPIQPKAIYSFQSGSVISQ
                     NGKPLLYYSKVIGQFKGLNQHESITLNLKNVEFTYPRSNFGLHNLSLTATSGELVGIM
                     GGSGVGKSTFFNLLNGSLTPQRGKISLNGVSYQDGLTQIQMLIGFVPQDDSLFENLTV
                     FENLYYTARLALGNLTVEQTKELVNAKLAEFGLYHIRNLKVGSSLSRNISGGQRKRLN
                     IVSEIIREPKLLLVDEPTSGLSSADSLRVMSLLKEQALSGRLVLVNIHQPSTEIYRLF
                     DKILVLDQGGYMVYYGNPLEAVRYFKMHAGRIDADEVECSVCKNIKSDEIFDIIDEKQ
                     VDEFGQLTENRKVKPIVWSSRFIPTDEGETTINPLSESKSIKTGWVKQLEVFLTRLTL
                     SKLRDIEFFIFAFVIPVLLAAVIAFFSKYSFPNEQGGVHLHLLRKL"
     gene            complement(9553..10197)
                     /locus_tag="PK049_09200"
     CDS             complement(9553..10197)
                     /locus_tag="PK049_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61776.1"
                     /translation="MKLNDYSSHSEILRQVGFESRTLLNGITGPIQLIRSLSNDPNLL
                     EILHILELSTVRFEKFSLRSQLLSDLLNPSMQVSNERFDLVDLLKHAVLELNDFINFF
                     GVIFELSKDLSSFEVEADRDIIYQSVLIVFEQFMSVLESGSAIKVDCEEHSQKISITA
                     FDNGFVADKFNQFSENQSVEIDVTLFVHSLKKFSIPFTVYKNNGHTVMEIARQQ"
     gene            complement(10248..11534)
                     /locus_tag="PK049_09205"
     CDS             complement(10248..11534)
                     /locus_tag="PK049_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HOK61777.1"
                     /translation="MLRKLPLYVKILIGMFLGLAFGFLVITFGIQHFATDYIKPFGTI
                     FLNLLKLIAMPLIFASLVSGVSGLSDVSRLSKLGIKTISLYIVTTIIAVTIGLVLVSV
                     IRPGSYVSEAKRVELLEKYSFDLAKRQQAAEGIAQQSPLQFLVDIVPENVVNAASNNT
                     SMLQVIFFAIMFGVALILVPHSKVAIVKDFVAGLNEVILKIVDIVMLFAPFGVFALMV
                     TLLVDFVGTDLGSSVQLFTSLGLYALTVILGLLLIITLVYPTFLKLYAKINFKKFYRS
                     IFPAQLVAFSTSSSAATLPVTMEQVEKELNVPNEISSFVLPVGVTINMDGTSLYQAVA
                     TVFIAEVFGVELTLVQLLTILITATLASIGSAGVPGAGIVMLIIVLNSVGLPVEGLAL
                     ILAIDRPLDMLRTTVNVTGDAMIATIIAKSESKLGE"
     gene            complement(11673..12197)
                     /locus_tag="PK049_09210"
     CDS             complement(11673..12197)
                     /locus_tag="PK049_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61778.1"
                     /translation="MKRKLLGMVLMALAIVTSCKNDNDDQDINIIRFNDGQFTLYRGY
                     SHKYSYALETGATPFVINLLGEGVSYSTDAGKFTGTGSLVTGYFYSENIAEVKNGLYT
                     IDIFSQKGKYTADSCKIYYNYDFAQDTGKIYTIKAGTFDVVNLGRLMSYKIDIQTADF
                     IHFTGEFRGTVDPL"
     gene            complement(12481..12975)
                     /locus_tag="PK049_09215"
     CDS             complement(12481..12975)
                     /locus_tag="PK049_09215"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOK61779.1"
                     /translation="MNVSKNKVVSISYELKVDGDLIDAAQADNPLVFLYGHGQLLPLF
                     EDNIKDLAEGDSFEFMIPSKDGYGEVNDQAIVELPKEIFVIDGELQEDLLVIGNRLPM
                     RDSEGNALDGTVVEVTDNSVVMDFNHPLAGKDLYFTGKVENIREATAEEISHGHVHGM
                     GHDH"
     gene            13186..13386
                     /locus_tag="PK049_09220"
     CDS             13186..13386
                     /locus_tag="PK049_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOK61780.1"
                     /translation="MKGKVKWFDAAKGYGFIQTEEGNDVFVHYTGIQREGFKRLDEGQ
                     AVSFEISEGKRGPQATNVVVLE"
     gene            complement(13441..14034)
                     /locus_tag="PK049_09225"
     CDS             complement(13441..14034)
                     /locus_tag="PK049_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61781.1"
                     /translation="MLKKSIFLVIFVSVSTLVFSQRKHGDFQRFSDRVYFGGSLGLAI
                     GNRITQIDVLPQGGIWIFPQWSVGLGGRYTYRRERFSFDPSSTSIKPVTTTIWGYSGF
                     TDILPIPDFNEAFGVKLHGGFVIHGEFENLYLDRNFFDITSTQGMGWIRMYMVGGGWR
                     QRIGDRAAVNFLVLWDLTDNRYSPYTSNPILRFSITF"
     gene            14123..>14403
                     /locus_tag="PK049_09230"
     CDS             14123..>14403
                     /locus_tag="PK049_09230"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOK61782.1"
                     /translation="MNIVITGASQGIGYATAIELAQKGNNTIFCIARNGHNLEKLQNE
                     VAKINPASTVIPVVYDLTKLCQGTEDIGSIIRKHIDKVDILINNAGLLYV"
BASE COUNT         4344 a         2904 c         2797 g         4258 t
ORIGIN      
        1 tgtgtgtcgc ttaaatctat ttctaacctg tagacttatt tcaggactag acaaggataa
       61 aggattagtt gatggatgct ggatgattgt tgatagtttg gtgttgcctc gtcttatcgt
      121 gtcatgttga gttcccgata tatcgggata gattccaccg aagcatatct tttctccttt
      181 taccctttga tgagattcct cgactagctc ggaatgacaa cactattggc tccctcactc
      241 cctttgtcat gctgagcacc gccgaagcat ctcatttaac ctccaataca tttatacctt
      301 ttgattagtt tccaagcagt cgctttttgg aatgtttttc cccatgcgat gcaacgtttc
      361 tttactgctt tttagattat gcacagcgag catcgctaat ttttttttat atatatcaat
      421 accctggttt aaaaccctgg tttaaagcga tttgccattt caggacgaat attgcacacg
      481 tctggagacg cgcgcaaaca atgtactaac ctttaaccaa atgcttttgc agcatgccaa
      541 ttagcttatc gcggaatatg ggtttggcaa tgtaatccac acatcctacc tcaaaagctt
      601 ttttcctatc gctctcggct gcgtaggctg tttgggcaat gattggtaca gtagggttga
      661 ttcgcttgaa ctcctcaatt acctcgtacc cggtcatatc gggaagtttt atgtccatta
      721 gaactacatc gtacgatgag ttctgttcaa aatggtcaat tgcactacta ccggtattct
      781 caatatgata gttaacactt aatccactaa aaagctcatt gaagtaggca atgttgaaag
      841 catcgtcctc cactaccaaa aggttcagat ttttaatcac ttccggattc acaggttcaa
      901 tctcaatttc ctcaactttc tttatgggct tgtaaggtat tgagaagtag aatgtggtac
      961 ctttgccaac ctcagactca agccatattt ttccgttgag caggttagcc aaactttgaa
     1021 caatggtaag tccaagtcca agcccgtcat aaatcactcc cttagggcta accttttgga
     1081 aacgctcaaa tattttatcc tgcatgtcgt gtggtatgcc aattcccgta tcggcaaccc
     1141 ggaatacaat tgaaccgttg gtaagctcat ggaatccaaa ctcaatgtaa ccactatgtg
     1201 taaactttaa ggcattatgg ataaggttaa caagtacctg gcgtagtttt ggctcatcgc
     1261 tttcaatcag gttcgatacg tgagcgggta aaggtaatag ccttaactca atttcctcac
     1321 gctccatacg ttttttgtaa ccctcaaagg tttgaaccag atcggtaaaa ataccattta
     1381 aatcaaaagg cttaaattca gccttaagct gtcccgattc gatacgggaa atatcgagta
     1441 gctcgttaac aatatcaagc aaatcgtatg agcgctgttt tattacgccc gtgtagtagc
     1501 taatcttatc ttcatcgtcg tggtccgatt caagaatttc agcaaagcca acaatggcgt
     1561 tcatgggtgt acgaatctcg tggctaatgt tctgcaaaaa agccgttttg agtttatcgc
     1621 tctcctctgc tttctccttt gcaattataa gttcgttaat taatcttctt ttgtcggtta
     1681 tgtcaatttt cactgaaagg taataggcgt ttttaccaag ttcatcggta ataggaacaa
     1741 ttattgattc ctcataaaaa acctcaccat tcttccgctt attgataaat tcacccttcc
     1801 aagtgctacc ggaagtaagt gtatcccaca attcaacata aagttcttta gatgtttgtc
     1861 ccgactgtaa aatacgtggg tttttaccca taacctcatg gggttcataa ccggttacct
     1921 tggtaaaggt agagtttaca tatacaattt cgggctttag agtggtaatt acaaccgata
     1981 cagggctttg ctcaacggct gtggaaagta actttacagt atcggccttg cgtttgtaat
     2041 caattgttcg tgctacctgc tcggagaaat actccaaaat gtattgctca ctctccccaa
     2101 aagcattgat attggtatag tgctgaaccg ccattacccc gagtatatta ccatggaatt
     2161 taaggggaac tcccaaccat gccattggtt ttgttccaat taggtcaatc tcatttttaa
     2221 aaattagctc atcgaagcaa cttggttggc aaagaagagt ttttcccgta cgaattacat
     2281 actcagttaa tccatggcca tccctacggg gatttggggt tttaatatcg gtagaatcga
     2341 caaagtaagg gaacgatacc tcattgtttt cggaattata aagggctata tagaagttat
     2401 cgatattaat taaatcggaa actattttat gggttaccgt aaaaatcctg ttcaacgaga
     2461 agttggtgct ggtcattcgg gcaatatcgt aaaccgcttt ttgcagcgcc ttgtcaattt
     2521 cttcgcgggt aacatccctt agaataacct caatggactg gaatgactgg ttatttttaa
     2581 aaccaggaat ataaatagcc tccacccact tataattatc gttaccaacg cttatcttga
     2641 atcgacgctt aatgaattca ttgctggttc cagcgttttc aaggattagc ttaatgcgtt
     2701 catcagggat aaacctgttc ctaagctctg gttgtgcttt atgctgctta aggttaaaac
     2761 ctaaaatgcg ctctgcctcc ctgttaatgt attcaacgta aacatctggc ttaagaataa
     2821 ccctgacaat accaaaggga aattcgctat gaattctatc gacatgggtg ctataccgat
     2881 caaatggagc gtagggtaat atgcaactat cggccttact ggtaaagtaa tattttaata
     2941 gtgttgcctg taaccattct ttactagtgc aatgtaccac atcgtcaata tccgtactga
     3001 gcaatttagc agaatcgagg tttaaggcat catggtcgag caaggcaaca acctgaactt
     3061 ggggagaaat atcattcctg accgacactg cttgcttaat taaaccgtaa ctattagaag
     3121 ttaaatgcac caataaaacg ccgttgaaac gctggcttaa aacattattt aaatcgggaa
     3181 gggaatcaac ctgtaaaaat ttacaatgtg aaaattcagg gcgattaagc aattccttta
     3241 cccattcatt acaatttgac tgattcgatg agtaaagcag tatatgccag ttactacctt
     3301 caacgggttt catcaagtaa aatgttaagg gttacagaaa tttgtaaaga gtacggtaaa
     3361 tcttatcgct gctaagcggt ttggttaaaa aatcggagca gccagaatca agagcttcag
     3421 ccctatcggc atcggtaaca aaggcggttt gagctataat gatagtgttt ggaaggaact
     3481 cctttattct tcgggttgcc tcatgcccac ttatgcccgg aagcttaagg tccatgaaaa
     3541 ttaaatcggg gttgtgagcg cgtgcctgct caaccgcctg ctcaccattg taagcagtta
     3601 caattttagc ctttgttttt gcaaggaaat tctggataat tagagaggta aacttatcgt
     3661 cctcaaccac cagaatggtt ttattgctta aatcttttat attctccatg gcactattct
     3721 ttaatggtaa aaaaacgaac cgctacaagc gatgagtaga aaacgcatgg caaaccctcg
     3781 cgataaaatc gatatttggg cgaaatggct ttgttgtcct caaaaattct caaccgtatc
     3841 ctgccaccac taatgcccat ttcctgcaaa tactccatca tggctttaag ccggctactt
     3901 gcaccaactg tatcgttttc aatctcgtga aggataccat taagttcaat tcctaagttg
     3961 gggttttgag tcatgaaatc agtaagccag taaagctgac aaaggctacg gggtttggtg
     4021 ccaggttgtc caatatcaaa gtaaacttca tcgagtagcg cataatatct acatagggta
     4081 tcaatttttg aaagttcatg ccttttaagc atgctaaagg gaatgaagcg aggcgagagc
     4141 tgctttacat cctctttttt aacaggctca agcctgatat tatctaggta atagtaagca
     4201 acctggttgg tgagtatggc gcgctgaagg gttgtgttta aggaactaat atcgtttggg
     4261 aaataggttg attgactatc agggaaaaag ttgcctatgg tcataaagaa ctcaccgccc
     4321 tgggttctaa aggtatcggc aaattcaacc caaattcccg gtgtgcagta tagtaccgca
     4381 gtgtcaataa taacagctgg tttgattggc aggacaccat ccttttgagt tattggactt
     4441 tcagtgaaag ctatgccaag cctattcgat acaaatgtag agtgctgcgc aagggcatac
     4501 ttaaagctaa tgcggtatag ctggttaggt tgcaggggtg taaccaactg cgtttgaata
     4561 tactccctgt agttaatagg tttaacctta cgcttagtat cgggcttttt aatattagcg
     4621 ttagtaatgt taacaggtgc aagggggccg ctatgtacat agtcacggta atcaatttcc
     4681 tccttcacat caggggtgtc gagcaacact aagccaataa agccatcgcc ctcggccggg
     4741 aaaatactac ccataaagtt ctggggtacc ccgaccatct ccttactaca acggttaaag
     4801 taatcgggag tacccttggt aggcattacc caattgggta aaaaacgttt tgtgtattca
     4861 accgtatagt tatcgggaca aacatagtat ttctcaaagc tgccattcct aatgagattc
     4921 tgccccgtta cagcaaaacc gaaaaataat gtgcatgata gtatggatat atataccctc
     4981 atagcttatg ctaaaaacct gttttaggga ctttgcgcca gtatattgct acaccggcac
     5041 atacccttga atcggttgcg tccttaaatt cctttttgct tgctaatacg gtaatctcaa
     5101 aaagaacgtt tttggtcttt tcaactgtaa atcctactgt ttctacgtag tcgtcgtttg
     5161 cattatcgta aaaaactgaa ttatcctccg cattaattat cctaaactga actggataat
     5221 aacccttttc agtacagata ccaattttat aatcgtaacc accaaaaagt acaacctggt
     5281 aggtaaaggt ttttttggct tcaaccatac ggcttcggga ctggccaaac cgcttgaaat
     5341 cactaatatc tggtggagta cattctctaa tagtatgaaa acctgtacat tgagcctgta
     5401 ccgttacaat agccgctata aatagtgaag ctgaaagtat aatttgctta aacatagcag
     5461 aaaggtttta ggttaatact taaggtcaat taacttttta cgagcattgg caaccatgtt
     5521 gcgcagctgt gccaaatcgg ctttgctaag ctttgtagta tcgccaccag ttatggtgaa
     5581 atgttccttg ctatcagtta ctaccgattc gttaccagct gaagatagtt tctccttgaa
     5641 aaacttgaaa atttcagtaa catcctcggt taaactttta aattcaggtt tactgctatt
     5701 gtatgcaata aagttattgt agtaattgtc aaaaacagcg ctctggtcaa taaccgtagc
     5761 tattgcttca tcggggtttg ctgcattctc aattgagtta atccctattt gcaatacctc
     5821 aatggcagca ccaataccaa tcattgagag tagctcccta ttgcccgatt caaggatagt
     5881 attgtatgcg ttttgcgaga aatcttcgaa aatggcatcc agtgagtctg ggttattaag
     5941 gtttgcgtcg aaacgcttaa agtaagcatc gttcagtatg gagcctatac ccaaatcgcg
     6001 agttagctcc tgtaaggctt taaagtattc cgaggaagca ttcctctggt tgtagtaatt
     6061 ggtataggca aagtcggcaa agtacacgcc taaaatcagt gccttgtgct taaccataac
     6121 cgcttttttc tcaataccaa ggggtgccat atagctatcg gaatacttaa ttcccgatgt
     6181 aagtacaatg ctcagcacct ccgatggcgt gggtagcata atctccattt gatcctgaac
     6241 taaagtatcc tcgctaagct cagtattaac atcagcttga ttggttttac ctcctgaaca
     6301 ggagtaaaac attgctgcaa acagcattgc taaaaccaca aaataaaatc tgtattgttt
     6361 cataaagcaa agtattaggt tcaatatttt catgttttac agttatcgcc aatttaagtt
     6421 tttccaattt aaaaagcaag tgataaaaaa caagaatgtg ttaaacgttc aaaaannnnn
     6481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaacca ttgaattgga gagttttggt
     6601 aaaatatcaa atagtaagat gaggtaaata cttacaagaa aaacaagaag cacgacaaca
     6661 ttaaaagtga aggtatcaaa tagctggttt gcaatgcgct tagctggagc aaggaagtgt
     6721 gctctacccc atctattcgt gggaatctta aaaaccggat cgattgtttg aaccagccta
     6781 ttctcaacca cctgaatggc tgttatattc ttgcgggcaa gagtcaaatc atctactccc
     6841 ctattggtgt gcatttgctt aaactccaaa aaggcttccg cactgtagaa ctggctctgt
     6901 agctttgtag taatttgatc cttttttcta atgatcctcg aatagttttg ttgttgtttt
     6961 tgctgtagtt catgaagtac aatttcaagc ttacgaaact cagcagtgtt aggctgtgga
     7021 aattcagcaa tatcaatatt tagttgacct aaaccgcttt taatgagatg tttgagccta
     7081 agccggttaa cgctatcggt ttgtttatag ctctgaataa gctgaaaaag ttttggaatt
     7141 aggtattgcg attgaatacg gctttggctt tcggccatat caatttcaaa aaaatgcctt
     7201 tcataggcat tattcttgaa ctgatgaaca agcaaggctt ccattcccca catggaggga
     7261 gaaactattc caatcacggg aacaacctca ggatttgcta accgatggtt catcctatca
     7321 aaaggaatgg ttataccgct taggataatc tggggaataa ccaggaatgg cacaataaga
     7381 taggcagccg ataacgattt gaaaatggtt gaaacaatga ggccggcgat attcccgaaa
     7441 aatcccatta aaatcagtat cacccaaata ggtaaaaaca ttccctttac cttgagtata
     7501 taaatagata ttgccgtgta tattagcgtt tgaaggattg ttaagccgaa gtaaaaaagc
     7561 acctttgaat tatagtaggc tttgcggttc aggaaaagaa aagcttcacg cttattgata
     7621 ttggcatcct taataacact atcggatgta actatcatac ccaagaacat ggacgcaata
     7681 atactggtaa gaaagaaaat gggtatgtta tagttttcgt aaaagatgta ggtgtacccc
     7741 cccctgttca ttcggaaacg aatacttgct gaaaaaggct ataacggcag caagtaaaac
     7801 gggtataaca aaagcgaaaa taaaaaattc aatatcgcgt aacttgctaa gggtaagcct
     7861 tgtgagaaat acctcaagtt gcttaaccca gcctgtttta atcgatttgc tttccgataa
     7921 tggatttatg gtggtttcac cttcatctgt aggtatgaag cggctgctcc aaactatggg
     7981 ctttactttt cggttttcgg taagctggcc aaattcatca acctgtttct cgtcaattat
     8041 atcgaaaatc tcatcggatt ttatgttctt gcaaacgctg cactccacct catcggcatc
     8101 aatgcggcca gcatgcatct taaagtaacg gacagcctca agcggattac cgtagtacac
     8161 catatatccg ccctgatcaa gcaccaaaat cttatcaaat aacctataaa tttcagtgga
     8221 gggttgatgg atgtttacaa gcactaacct tccagatagc gcttgctcct tgagcaacga
     8281 cataactcta agcgagtcgg ccgatgacaa gcccgatgtt ggctcgtcga caagcaacaa
     8341 ctttggctcc ctgatgattt cggaaactat gtttaacctc ttgcgctgtc caccactaat
     8401 attacgcgaa agcgaactcc caacttttag gttacgaata tggtatagtc caaactcagc
     8461 gagcttagca tttactagtt ccttggtttg ctcaacagtt aaattaccta gggcaagccg
     8521 tgcagtatag tataaattct caaaaacggt taggttttca aaaagcgaat cgtcctgggg
     8581 aacaaaacct attaacattt ggatctgggt caaaccatcc tgatagctaa caccgtttaa
     8641 agaaatctta cccctttggg gtgtcaatga gccattgaga aggttaaaga aagttgactt
     8701 tccaacccca cttcccccca ttattcccac cagctcaccc gacgtggcag ttagcgacaa
     8761 gttgtgtaat ccaaaatttg aacggggata ggtaaactca acatttttta ggttcagggt
     8821 tattgattca tgttggttta atcctttaaa ctggccaata accttactat agtacaaaag
     8881 cggtttgcca ttttgtgaaa taactgaccc actctgaaat gagtaaattg cttttggctg
     8941 tatgggcttt gagtttagct gtaggttttc ctttccctgg taacggaaaa agattgtaga
     9001 aacatcggga aagtaaacaa atacaaaata cccatcaatc tgttcccttt ttacaaaatg
     9061 aaagccttcc aattcagggc ttaccgactt tactattagg atattcctac ggtcgtgtac
     9121 ttcgtaaagc ttgcccgaaa caaagctgct gcataagcta taggtttcag ccgatatttt
     9181 taactcatcg gctatctgtt ttattttacc agcaatacta tttgattttg atagtagaat
     9241 tgcatttttc tgaacaaata agccaaattc aagaagattt agaagaagta taagcctttg
     9301 ctgaattgaa ctttcactat ttaatttgct acaaatcttt ttaacatcaa tgctactatt
     9361 ggcttgcttg ctattcctga ctacctgttt tagctcctct ttctgttttt tcccaatcct
     9421 gggatttaca aggcgggtaa aaagaacttc agtgtaaatt tctgcaagcc tttgcaggct
     9481 ggcatcgcca ttaagcacta tatgggcaaa aaggttaacc tgtgaattta ccaaaaactc
     9541 gttcatggct ttctattgtt ggcgagcaat ttccattaca gtatggccat tattcttgta
     9601 gacggtaaag ggtatgctga attttttaag gctatgcaca aacagggtaa catcaatctc
     9661 taccgactgg ttctcgctaa actggttaaa cttgtcggca acaaacccat tgtcgaatgc
     9721 tgttatggaa attttctggg aatgctcttc gcaatcaacc ttgatggctg aaccgctttc
     9781 aagaactgac atgaactgct caaaaactat cagaactgac tggtaaataa tatctctatc
     9841 ggcttcaacc tcaaaacttg ataagtcctt gcttaattca aaaataacgc caaagaagtt
     9901 gataaaatca tttagctcca gtacagcatg ttttagtaaa tcgaccaagt caaatcgctc
     9961 gtttgatact tgcattgatg ggtttagtaa atcggaaagg agctgcgagc gtaacgagaa
    10021 tttttcgaac cttaccgtgc ttaattccag gatgtgaagt atttcaagga ggtttgggtc
    10081 gttgcttagt gaacgtataa gctgaattgg gccggtaatg ccattaagta gagttcttga
    10141 ctcaaaccca acctggcgta aaatctcgga gtgacttgag taatcattta gtttcatggc
    10201 taacttatgt cagcctaagg taataaaaaa agctgaaagt tgttcatcta ctcaccaagt
    10261 ttactctcgc ttttagcaat tattgtagca atcattgcat cgccggttac atttactgtt
    10321 gtacgaagca tatcgagtgg gcggtcaatg gcaagtatta aagccaaacc ctcaacgggc
    10381 aagcccacag agttcagaac aataattaac attacaatac cagctccggg cacacccgct
    10441 gagccaattg atgccaaagt tgccgtaatt agtatggtta gaagttgtac taaagtaagt
    10501 tcaaccccaa aaacctcggc aataaaaacg gttgcaacag cctggtaaag acttgtccca
    10561 tccatattta tggtaacccc taccggcaat acaaagctcg atatttcatt gggaacattc
    10621 aactcctttt ccacctgctc catggtaacc ggtagtgtgg cggcgctcga gctggtagag
    10681 aatgccacta gctgagcagg aaaaatggat cggtaaaatt tcttaaagtt gattttagcg
    10741 taaagcttta aaaatgtggg gtatactaag gtgataatta ataataatcc aagtattacg
    10801 gttaaagcgt ataaccccag cgaggtaaaa agttgaaccg atgaccctaa atcggttcct
    10861 acaaaatcga ctaaaagagt taccattaat gcaaacacac caaaaggggc aaaaagcata
    10921 acaatatcca ctattttgag aattacctca tttaaccccg caacaaaatc ctttactata
    10981 gccacctttg aatggggaac aagaattaac gcaacaccaa acattatggc aaagaaaatg
    11041 acctgcagca tggaagtgtt attggatgct gcgtttacaa cattctctgg aactatatcc
    11101 acaaggaatt gcagaggtga ttgctgtgcg atgccctcgg cggcttgttg ccttttggct
    11161 aaatcgaacg agtatttttc aagtaattca accctttttg cctcactaac atagcttccg
    11221 gggcggataa cactcactaa aaccagccca attgttacag caattatggt agtaacaatg
    11281 taaagcgaaa tggtttttat tcctagcttt gataaccgac taacatcgct taagccgcta
    11341 accccactca ccaacgatgc aaatattagc ggcatggcaa taagcttaag caggttcagg
    11401 aaaatggttc caaaaggctt gatatagtca gtggcaaaat gctgtattcc aaaagtaatt
    11461 accaaaaatc caaatgccaa gccaaggaac atgcctatta aaatcttgac gtaaaggggt
    11521 aatttgcgaa gcatagacag gaatttaagg ataaaggata aaggataaag gataaaggtt
    11581 aaaggttaaa ggataaaggt ttaaggttta agtttaaaat ttcagatttc agatttcaga
    11641 tttcaataat attcaaattt ctagtttcat agctaaagag gatcaactgt gcccctaaac
    11701 tcgccggtaa agtggataaa atcagcggtt tggatatcaa tcttgtagct cattagccta
    11761 cccaggttaa ccacatcgaa tgtaccagcc ttaatagtgt atattttgcc ggtatcctgt
    11821 gcaaaatcgt agttgtagta tattttgcat gaatcggcag tatattttcc tttttgtgaa
    11881 aagatgtcga tggtgtaaag accattctta acctcggcaa tgttttctga gtaaaagtat
    11941 ccggttacta aggatccagt cccggtaaat ttaccggcat cggtgctgta gcttactccc
    12001 tcaccaagca ggtttataac aaatggagtt gcgcctgttt caagggcata gctatactta
    12061 tggctatagc ctcggtaaag ggtgaactgt ccatcgttaa acctgatgat atttatgtcc
    12121 tggtcgtcat tatcgttttt acagctggtt acaatggcca gcgccatcag aaccattcct
    12181 aaaagtttac gtttcatacg tacaaaatat ttatattatt aattactaaa tcattactct
    12241 cttgctttta acaaacatta tcgagcggat ggaacaccca tagtttgact ttgtcgagct
    12301 cgcaaaaaac gctcggttgt tcatctgtgt ttgtgcaagc taccttccat gggcgtttca
    12361 taatctgaaa atattaggca agtaatttaa tattactgct aaaatagtaa aaatgttaca
    12421 taatgtaaaa aaggaggtga ataattcacc cccttttact tacaaaagct gaaatatctg
    12481 ttagtggtcg tggcccattc catgcacatg accatggcta atctcttcgg cagtagcttc
    12541 gcgaatgttc tcaaccttac cagtgaagta aaggtccttt ccagctaggg ggtggttaaa
    12601 atccataacc acggagttat cggtaacttc aaccactgtt ccatccagcg catttccctc
    12661 ggagtcgcgc atgggtaagc ggttaccaat caccaggaga tcttcctgga gctcaccatc
    12721 aataacaaaa atctccttgg gtagctcaac aatggcctgg tcgttaacct caccgtaacc
    12781 atccttgctt ggaatcataa attcaaagga atcgccttcc gcgagatctt taatattatc
    12841 ctcaaaaaga ggcaagagct ggccatgccc atagaggaat acaagtggat tatctgcctg
    12901 tgcagcatca atcaaatcgc catcaacctt tagttcatag ctaattgata caactttgtt
    12961 tttggaaacg ttcatgtaaa ttgttttaga atattaattc atcaaagaaa ctactttctt
    13021 ttggtataaa caagcgtgta aggttaattg ttcgcattta aggttgataa attttggtca
    13081 taggtaaaca aaaaacataa aatgatgttg tttattcaaa taatagttac atatttgtaa
    13141 tgttttaaaa caatcaattt attcacaatt tatttttaac gtaacatgaa aggtaaagta
    13201 aaatggtttg atgcagcaaa aggctatggc tttattcaaa ccgaagaagg taacgatgta
    13261 ttcgttcact acacaggaat tcaacgcgaa ggttttaaac gcttagatga aggtcaagcc
    13321 gttagcttcg aaatttctga aggcaaacgt ggtccacagg ccacaaatgt agttgtgtta
    13381 gaataacatt tattcctaac ataataaaag aaaaggcctc tgtttgaggc cttttttatt
    13441 ttaaaacgtg atagagaacc gaagaatagg gtttgaagtg taaggcgaat agcggttatc
    13501 ggttaaatcc catagaacca aaaagttaac ggctgcccta tcgcctatcc gttgacgcca
    13561 gcctcctcct accatataca tccgtatcca tcccatgcct tgggtagagg taatgtcaaa
    13621 aaaattacgg tcaaggtaaa ggttctcgaa ttcaccgtga attacaaaac caccatgaag
    13681 cttaacgcca aaggcttcat taaaatcggg gatgggtaaa atatcggtaa aaccagaata
    13741 tccccatatt gtagtagtta ccggttttat actggtgctc gaggggtcaa agctaaacct
    13801 ttccctgcga taggtataac gcccacctag cccaaccgac cattgtggaa aaatccaaat
    13861 gccaccctgg ggcaatacat caatttgggt tatgcgattt ccaatagcta ggcctaaact
    13921 acccccaaag tatactctat ccgaaaatcg ttggaaatcg ccatgttttc gttgcgaaaa
    13981 gacaagggtg cttaccgaga caaatattac tagaaagata gattttttca gcataatact
    14041 gatgtttact tactaatatg aacacaatat tagccattta tgtttaattg gttaacttta
    14101 tgtctgttaa aaaagattag cgatgaacat agttattact ggtgcctcgc agggcatagg
    14161 ttatgcaact gcgattgaac tagcccaaaa aggaaataac accattttct gtatagcccg
    14221 aaacgggcat aacctagaaa aactacagaa tgaggttgcg aagattaacc cagcctcaac
    14281 cgttatccct gtagtttacg acttgacaaa actctgccaa ggaactgagg atattggctc
    14341 aatcataaga aagcatatcg acaaggttga tattctgata aataatgcag gattgcttta
    14401 tgt
//
LOCUS       DAOSAL010000069        14398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_2855_length_14398_cov_52.075647, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000069 DAOSAL010000000
VERSION     DAOSAL010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 14398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14398
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2855_length_14398_cov_52.075647"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..974)
                     /gene="miaA"
                     /locus_tag="PK049_09235"
     CDS             complement(51..974)
                     /gene="miaA"
                     /locus_tag="PK049_09235"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOK61783.1"
                     /translation="MLDSKYDLVAIVGPTASGKTQLAAHLAARVNGEIISADSRQVYR
                     GMTIGTGKDLDDYVVEGRLIPYHLIDIVDAGYKFNVFEYQQHFIDAYNDIKKRNRLPI
                     LCGGSGLYIDAVLRGYKLLPVPENPELRRELEAKTDEELATILASYKKLHNTTDLDTR
                     KRTIRAIEIEEYYQRQPVDPKPFPQIRSIIFGVKHQRAAEMKRIRERLAQRLNAGMID
                     EVKALLNAGLTPDDLIYYGLEYKYITEYLIGKHSYDEMFEKLYIAIRQFAKRQMTWFR
                     GMERKGFTINWIAGELTLEEKISLVLNKINE"
     gene            1037..1297
                     /locus_tag="PK049_09240"
     CDS             1037..1297
                     /locus_tag="PK049_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61784.1"
                     /translation="MVKLRHPNPTNKLNELNRLVWFGLLLFVSGTAAILIATNFFSQP
                     LLIAKNYMLIAIIAILLMLYVYRLVGITRKPLKTPETHGKQE"
     gene            1281..1736
                     /gene="smpB"
                     /locus_tag="PK049_09245"
     CDS             1281..1736
                     /gene="smpB"
                     /locus_tag="PK049_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOK61785.1"
                     /translation="MENKNNIVIKNKKATFQYEIIETFTAGIVLKGTEIKSIRMGKAS
                     FVDSYCHFIGEELWIKGLHIAEYAWGTYNNHDPKQERKLLLTRKELIKLKRRSEEKGL
                     TIIALKLFINQRGLAKVDIALARGKQLHDKREDLKHKDARREMDRAMKR"
     gene            complement(1756..2370)
                     /locus_tag="PK049_09250"
     CDS             complement(1756..2370)
                     /locus_tag="PK049_09250"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOK61786.1"
                     /translation="MKVKNLILLVLTTMSVNLAAQDFAKLSVAFTESYAKEKEGKYAD
                     AAKPLKAMYDEKSYEVNLRLGWLTYLQGQFSESVGYYSKAIELMPYAIEPRLGIVLPA
                     SAMGNWGSVVEQYNKILSIDPNNTLVLYRMGMISYENKDFKKAYTYFEKVVNLYPFDY
                     YSVLMLGWTNYQLGKTKEAKILFQKALLYDPTSASAKEGLSLLK"
     gene            complement(2373..3641)
                     /locus_tag="PK049_09255"
     CDS             complement(2373..3641)
                     /locus_tag="PK049_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61787.1"
                     /translation="MRTIIVIVSVLVLTYRLGAQTDIASLDRLNYQYFLGKDYKNLKR
                     TAKEILNQGNDYYYLRMRLGILAYENQRYAYAYRNFTRAKQQNPFDTVTNEYLYYCYL
                     LAGRQADALQFLRTLSENDKNSHLKSLPMAKAPELILSYSYTGYDVKKYHTNPLNFEA
                     IENLAVLNAGLAFNFSERANGFFQYSNTSKVATFYSPSDPSGNYDTYKQNQFYFSYKS
                     QITQGFEFSGYTHWVFFTDVSNVSTFGRRSSSINLSTESLFGLNFTLIGWYLRGVINF
                     NYSNFANSNQFATEAYITYLPFSNLNLYSTVSGFYQLDKNWGGTYQSNFDVGFKVFKY
                     LWVESGMMIGNSFLYSRNLGRELNNSFIIPSKSFYGSLVIPAKKFFLRVGGHYSEINN
                     YSWDLVNYTKTSKLVLNAFGVNATLTIKFQ"
     gene            complement(3629..5803)
                     /locus_tag="PK049_09260"
     CDS             complement(3629..5803)
                     /locus_tag="PK049_09260"
                     /inference="COORDINATES: protein motif:HMM:NF015226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015204 - urea transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071918 - urea transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HOK61788.1"
                     /translation="MRAINGLNTIIKGLIRSYSQIFFSSEYSLAILLLLITFIDFSIG
                     IAGLVSALVATLTSYLIKLDRETTAKGLYGFNALLVGFGLGFYYDISITLLLLAAIAG
                     FFTLLVNITLQGVLGKYYLPYLSLPFVLSMWLVLSATWQLTSTEINQSSVYLLNRIFD
                     VGGFNFIAIHDWWLNHVKSNFINGYFISLGSIFFQFNVLAGVLVALTLMAYSRIAFSL
                     SVIGYSVAYIAYLTFGLDLTTLGYSYIGFNFILSAIAIGGYYYIPSTASYWWAVAITP
                     VIAMVTIGLAIIFKTLGLPILSLPFNIVVLMFIYSMRFRISVSGLREVMVQEGKPELN
                     LYSYQSFAKRFPNYGWIRIRLPFYGVWTVNQGHHGNYTHKGEWAHAWDFIVLDSDGKQ
                     FKSDGHLQTDYYCYGKNVIAPADGEVVEVDDGVDDNPVGEININKNWGNTVIIRHAAG
                     LYSKLSHLRKGSITVKVGDKVKFGTKVGEVGNSGRSAYPHLHFQIQATEYIGSKTIKY
                     PLSSYIVNDTELKTFDYPATDQNVCNIESDPLLNSMLNFKPGKLISWEVQHENITENI
                     TWEVGITYLNKLFIKCNKTNSKAFFELDDVYFYFTHFEGSKKSLLYKFYLAAYRIPLT
                     YFEGIKVSDSIPVNKWFGGIALYLHDFIAPFIQLLQAEYRVKVKRIGSEFDIEGFRFE
                     TTVEGRFLNRTVLTERFDLEIRANGKVEIIQEQNNTLAKCEL"
     gene            complement(5823..7169)
                     /locus_tag="PK049_09265"
     CDS             complement(5823..7169)
                     /locus_tag="PK049_09265"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOK61789.1"
                     /translation="MEKKNFERPTITKLTPGMPSKHGVGIAVAPVQFIDGIKVDELIE
                     KYGSPLYIFSESVIRKNVNDLKRAFSVRYPKVQIAWSYKTNYLDAICRVFHQEGSWAE
                     VVSGFEYEKARLNGIPGEHIIFNGPGKTEAELLRAAKEGAYIHIDHFDELFLLAKVCQ
                     ANSVNARVAIRVNMDVGIYPKWDRFGFNYENGEAWDAINRIMANPQLKLMGLHTHIGT
                     YIMTTDAYRIAAAKLSELAASIAQKFNHYIAYIDMGGGFASRNTLRGAYLSGEDTTPS
                     FDEYADAITSAIINSQIKPDKLPTVILETGRALIDDAGSIAGTVLANKRLTDGRRALI
                     VDVGVNLLFTSFWYDHKINPTQSHSGLVEETVIYGPLCMNIDVLRPSIMFPPLKTGEH
                     FLISRTGAYNNTQWLQFITTRPNVILIDTNRKTHIIRKAESIDSIVSNENIPDHLK"
     gene            complement(7190..8236)
                     /locus_tag="PK049_09270"
     CDS             complement(7190..8236)
                     /locus_tag="PK049_09270"
                     /inference="COORDINATES: protein motif:HMM:NF026968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOK61790.1"
                     /translation="MERKKITIAITGLNNIDSPGPGMPVIRGIKESTDFDTRIVGLAY
                     DHLEPGIYMKEWVDKTYMIPYPSKGKEALFERIKEINAKENIDLIIPNFDAELFSFIK
                     LEKQINQLGIKTFLPTLEQFEERHKSSLPEYGKKYGVNVPKSEEAYSMNDLANVLRSY
                     TYPVMVKGKYYDAYKAYSQEQAQAYFLKVSAKWGYPVIVQEFVEGTEVNVIALGDGNG
                     QVISAVAMRKQYITDKGKAWGGVTIADDSMLELTHKIISQTKWRGGMELELIRTADNK
                     LYILEINPRIPAWVYLAVGAGQNLPEALVKLAMGIDVKPYTSYEIGKMFIRYSWDMIV
                     DIDMFSKLVTSGEL"
     gene            complement(8241..8486)
                     /locus_tag="PK049_09275"
     CDS             complement(8241..8486)
                     /locus_tag="PK049_09275"
                     /inference="COORDINATES: protein motif:HMM:TIGR04353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr-rel-A system PqqD family peptide chaperone"
                     /protein_id="HOK61791.1"
                     /translation="MKVNQSIAISENGFIFNPITGDTFTLNDSGFQILTMLKSGKSQD
                     EIKDKLLSEYEVDELVLERYLQDFISDLKRNNLLLEE"
     gene            8628..11414
                     /locus_tag="PK049_09280"
     CDS             8628..11414
                     /locus_tag="PK049_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61792.1"
                     /translation="MKWLKIIALLPFFFSSVSSWCQVDSIGAKSGGMQRAMADSLLNA
                     KSYTKAIPKYKELLVQFPRDPEIKYALGVCYLYGTRDVEMALKYLKDASTSDLNTMVY
                     FYLAEALRQSYQFNEAIDYYRRFVVSGGSPDIKLDEVEKLVTACENGSFLTRYVYSPT
                     VFDRKQASPSNFLNYYSSLLPEGSFTPVPENLRTVTDKQMGYTSVMFYPKNIKPGDYL
                     YYSSYGKSTSWGTDIFRIQLLDNGKWSKPENLGDIINSSLNEEYPFIMPDGVTLYFAS
                     KGHYGMGGYDIYKSVYNADKKQWSTPENLGFPVNSTYDDFLFLTSNNDTLACFTSNRL
                     SMPDTLNVFLIRNDINPTRRTPNSYKELLSIARLNPVESKPSASPKVPSSKVETDKPK
                     AEAVENKKPAKFNSVENDPEYSRVLAKGFAAQKLADSLKIKLEDLRGRFDYVTTAEQR
                     VRLEKQVTKVEDDMLAAQKEADLLFARASQIEQEYLTGKRKPKDESNTSFVADNPDFI
                     YQAQFASTVFRTDEINRLADAEKLTPQLIRFRNFALERRDAYNRCLSANDNNNACTAE
                     YNQLVAAMKQYSDHFKKYWDKKYQIYSDCIEVARVKSGNRDTEVRKLTTDAQNNFRAA
                     MAILNNLDSTGQVESVFEASLLRDLGLMQFDLAFAKIWRLRLMEQQLLSNVIKLERNI
                     FGQSSLNLAQEEQKPTKTVEEVEVDMPKLKSEPAIKQATPTIAIIDEIPADFGVTDKP
                     YYSDEKPIPIDEPLPSGVIYRIQIGAFSAPQKPSFFKGMVPVYGYKTGNIIRYYIGNL
                     TRYSDAEKALVTVKQKGFKDAFIVAWLNGTRITTQRAQQLEDAVQQVQQSKTDSRLYI
                     VEVGRYKTPLTDSEINTVRNLSQGKELTRKVDSNGDHIYIIGNFNSIDEANRVKENLI
                     ASGMLKAVVIDITP"
     gene            11450..11731
                     /locus_tag="PK049_09285"
     CDS             11450..11731
                     /locus_tag="PK049_09285"
                     /inference="COORDINATES: protein motif:HMM:NF014655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HOK61793.1"
                     /translation="MAVKEKVQGKGKLLPSAELEYILILHNDDVNTFDFVIDTLVEVC
                     NHDPHQAEQCALITHFKGKCDIKHGEFEMLEGMMNELHRRGLSATIASN"
     gene            11742..12200
                     /locus_tag="PK049_09290"
     CDS             11742..12200
                     /locus_tag="PK049_09290"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOK61794.1"
                     /translation="MFWIIALIILGLFLLVVEFFMIPGVTVAGIGGFLIIIYAVYSAY
                     ADHGTTAGHLTLLFTAIASIATLVFSLRARTWKRVSLETSISSKAVEELENEIHVGDE
                     GVTISRLAPMGKASIKGKTVEASTIGELINPNTPIVVVHVETNKIIVKPK"
     gene            12210..13190
                     /gene="floA"
                     /locus_tag="PK049_09295"
     CDS             12210..13190
                     /gene="floA"
                     /locus_tag="PK049_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018695856.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HOK61795.1"
                     /translation="MNEVGALLLIIALSVVGLWIIFYFVPLGLWFQALVSGVRISLLQ
                     LIFMRWRKVPPRVIVQAMIEGTKAGLTLNRNEMEAHFLAGGRVSKVVHALVSAQKANI
                     PLDFKMATAIDLAGRDVFEAVQMSVNPKVINTPPVTAVAKDGIQLITKARVTVRANIK
                     QLVGGAGEETVLARVGEGIVTAIGSSLSHKEVLENPDKISKTVLDKGLDAGTAFEILS
                     IDIADIDIGRNIGAVLQMDQANADKNIAQAKAEERRAMAVALEQEMKAKAQEARAKVI
                     EAEAEIPKAIAEAFRSGNLGVMDYYRIKNIEADTNMRDNIAKSGNEPKKK"
     gene            13322..13410
                     /locus_tag="PK049_09300"
     tRNA            13322..13410
                     /locus_tag="PK049_09300"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13356..13358,aa:Ser,seq:gct)
     gene            13427..13500
                     /locus_tag="PK049_09305"
     tRNA            13427..13500
                     /locus_tag="PK049_09305"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13461..13463,aa:Pro,seq:tgg)
BASE COUNT         4280 a         3120 c         2842 g         4156 t
ORIGIN      
        1 ggtttattcc gacaacgatt tactgatatt caaaagaaac gagcgtaagg ctattcgttg
       61 attttattaa gaaccaacga aattttttcc tcaagggtca actcaccagc tatccagtta
      121 atggtaaacc ccttacgttc catcccccgg aaccaggtca tttgcctttt agcaaattgc
      181 cgtatggcta tgtaaagctt ttcaaacatt tcgtcgtagg aatgttttcc aatcagatat
      241 tcggtaatat acttgtattc aagcccatag taaataagat cgtcgggagt gagacctgca
      301 ttcaagagcg ccttcacctc atcaatcatg ccagcattta agcgctgagc aagacgctcc
      361 ctgatacgtt tcatctcggc agcacgctgg tgtttcacac caaaaattat gctcctaatt
      421 tgcgggaatg gcttaggatc aacaggttga cgttggtagt actcctctat ctcaatcgcc
      481 ctgattgtcc ttttacgggt atccaaatcg gtggtattat gcaatttttt gtacgatgca
      541 agaatggtag ccagctcttc atctgtctta gcttccagct ctcgtcggag ttcaggattt
      601 tcaggaactg gcaatagctt ataccctcgc agcaccgcat cgatatagag accactccca
      661 ccacaaagta ttggtaggcg gttgcgtttt tttatatcgt tgtatgcatc aataaaatgc
      721 tgttgatact caaaaacgtt aaacttatag cccgcatcaa caatatcaat aagatggtaa
      781 gggattaacc tcccttcaac aacataatcg tccaaatcct ttccggttcc aatggtcata
      841 cccctataaa cctgtcggga atcggcgcta attatttcgc catttacccg ggcggccagg
      901 tgagcagcga gttgggtttt accacttgcc gttgggccaa ctatggcaac taaatcatat
      961 ttactatcca acatctacac aacagtagtt aagcaggtaa aaatagctaa atttgcagac
     1021 ctaaaggttg tgagcaatgg taaagttacg ccaccctaat ccgacaaaca agctgaatga
     1081 gctaaaccgt ttggtatggt ttggtttatt actatttgtt tcgggtacag cagccatact
     1141 tattgcaacc aacttcttta gccagccact actaatcgca aaaaactata tgctaattgc
     1201 aattattgct attctgctga tgctttacgt ttaccggcta gtaggaatta cccgtaaacc
     1261 acttaaaacc cctgaaacac atggaaaaca agaataacat agttataaaa aacaaaaagg
     1321 ccacgtttca gtatgagatt attgaaacct tcaccgcagg aatagtgctg aaagggaccg
     1381 aaataaaatc gataagaatg ggtaaagcca gctttgtgga ctcttactgc cactttatag
     1441 gcgaagagct ttggattaaa gggctccaca tagctgagta tgcctggggt acatacaata
     1501 accacgaccc caagcaggag cgtaaactac tgctcacccg aaaggaactt attaagctga
     1561 aacgacgttc ggaagagaaa gggttaacca tcattgccct aaagctattc atcaaccagc
     1621 gtgggcttgc aaaggttgac attgcccttg cacgcggtaa acagttacat gacaagcgcg
     1681 aagaccttaa acataaagat gcccggcgcg aaatggaccg ggctatgaaa cgttaaatcg
     1741 ccaaaacaat tgatactatt tcagtaagct taatccctcc ttagcgcttg cgcttgtagg
     1801 gtcgtaaagt aaggcctttt ggaaaagtat cttggcttcc tttgttttcc ctaactggta
     1861 attagtccaa cccagcatta gtacgctgta atagtcaaag gggtaaagat ttactacctt
     1921 ttcaaagtaa gtgtaagcct ttttaaaatc cttattctca tagcttatca tacccatacg
     1981 atacaacaca agagtattat tagggtcaat ggaaagtatc ttgttatact gctcaactac
     2041 tgaaccccaa ttacccattg ctgatgcggg gagtacaatt cccaaccggg gttcaatggc
     2101 atagggcata agctctatag cttttgagta gtagcctacc gattcgctaa attgaccttg
     2161 caggtatgtt aaccatccca accttaaatt aacctcatac gatttttcat cgtacatggc
     2221 ctttaggggt tttgcagcat cggcatactt gccttccttt tccttagcat agctttcagt
     2281 gaatgctact gatagtttag caaaatcttg agccgctaag tttaccgaca tagttgtcag
     2341 caccagtaag attagatttt tgaccttcat agctattgaa atttaatggt taatgtagca
     2401 tttactccaa atgcattaag tactagcttt gatgttttag tataattcac caaatcccag
     2461 gaatagttgt ttatctcaga gtaatgaccc ccaaccctta aaaaaaactt tttagcaggg
     2521 ataactaaac taccataaaa acttttagaa ggaatgataa atgagttgtt cagttccctg
     2581 cctaaattac gtgaatacaa gaaagaattg ccaatcatca tcccggattc aacccataaa
     2641 tatttaaata ctttgaaacc aacatcaaaa ttcgactgat aagtacctcc ccagttttta
     2701 tccaattggt aaaaaccact aaccgttgaa tacaggttta aatttgaaaa aggcaaatag
     2761 gtgatgtaag cctccgtagc aaactgattt gaatttgcaa agttgctgta gttgaaatta
     2821 ataacccctc tcagatacca acctattaat gtaaagttta atccaaataa cgattcggtt
     2881 gataaattta tggaagaact cctgcgccca aaagtagaaa cgttcgatac atcggtaaaa
     2941 aatacccagt gggtataccc tgaaaactca aaaccttgag taatttggct tttgtaacta
     3001 aaataaaatt gattttgttt gtatgtatcg taattgcctg agggatcgga gggtgagtaa
     3061 aaagtagcta ctttactggt attagagtac tggaaaaaac cgttagccct ttctgaaaaa
     3121 ttgaaagcta atcctgcatt taatacagcc agattttcaa tggcctcaaa gtttaagggg
     3181 ttggtgtgat attttttgac atcgtatcct gtatatgaat agcttaatat tagttcagga
     3241 gccttagcca tgggtaagga ttttaaatga ctatttttat cgttctcgga aagggtgcgg
     3301 agaaattgaa gtgcatcagc ctgcctgcct gccagaaggt agcaatagta caaatattcg
     3361 tttgttacag tatcgaaagg attttgctgt ttagcgcgag taaaatttcg atacgcataa
     3421 gcataccttt gattttcata ggccaaaata cctaacctca tccttaggta gtagtaatca
     3481 ttcccctgat ttaaaatttc tttggcagtc ctttttaaat tcttataatc cttaccaagg
     3541 aaatattgat agtttaacct atcaagcgat gcaatatcgg tttgtgcacc tagcctgtag
     3601 gttaacacca aaactgaaac tataacaatt atagttcgca tttcgcaagg gtattatttt
     3661 gttcctgaat aatttctact ttaccgtttg cacgaatttc taaatcaaat ctttctgtta
     3721 gaactgttct atttagaaat ctgccctcaa cggttgtttc gaacctaaaa ccttcgatgt
     3781 caaattcgct tccaattctt ttaaccttta ccctgtattc agcttgcaat aattgaatga
     3841 atggcgcaat aaaatcatgg aggtaaagag ctattccacc aaaccattta tttacgggta
     3901 ttgaatcaga tacctttatc ccttcaaaat aggttaaggg tatcctatat gcagcaaggt
     3961 aaaatttgta taatagtgat tttttacttc cctcaaaatg ggtaaagtag aaatacacat
     4021 catcaagctc aaagaatgct ttagaatttg ttttattgca ctttataaat agcttattta
     4081 aataggtaat acccacttcc cacgttatat tttcagtaat attttcatgt tgaacttccc
     4141 agctaatcaa cttgccaggt ttaaagttta gcatagaatt aagcaatgga tcagattcaa
     4201 tattgcaaac attttggtca gtagcgggat aatcgaatgt tttgagttca gtatcattaa
     4261 caatgtatga tgataacggg tatttaatgg ttttggagcc aatgtactcg gttgcctgaa
     4321 tctgaaaatg gaggtggggg taagctgaac gccctgaatt gccaacctca cctacttttg
     4381 ttccaaactt tactttatcg ccaaccttaa cggtaattga accctttctg aggtgcgaaa
     4441 gcttggaata caaaccagct gcatgcctaa ttattacggt atttccccaa ttcttattaa
     4501 tatttatttc acctacagga ttatcatcaa caccgtcgtc aacctcaacc acttcgccat
     4561 cggcaggagc aattacattt ttcccgtaac aataataatc ggtttgtaaa tgaccatcac
     4621 ttttaaactg tttaccatcg gagtctaaaa caatgaaatc ccatgcatgc gcccactccc
     4681 ctttgtgagt ataattgcca tgatgacctt gatttacggt ccaaactccg taaaaaggta
     4741 acctaatcct aatccaccca taattgggaa aacgtttagc aaacgattga taggagtaaa
     4801 gatttagttc gggtttccct tcctgcacca tcacttccct taacccagaa accgaaatcc
     4861 taaaacgcat ggagtaaata aacataagta caacaatatt aaagggtagc gaaaggattg
     4921 gcaagccaag cgttttaaaa ataattgcta gcccaatagt taccattgca attacaggtg
     4981 taatggccac agcccaccag tacgaagccg ttgagggtat atagtaatac cccccaatgg
     5041 caattgcgct aagaataaag ttaaacccaa tatagctata ccccaacgta gtcaaatcta
     5101 gaccaaaagt taaataggca atataagcca cgctgtaccc aataaccgat aacgaaaatg
     5161 caattcgtga gtatgccatc aatgtcaatg ctaccaaaac cccggctagc acattaaact
     5221 gaaagaatat agagcccaac gatataaaat aaccattaat gaaattggat tttacatggt
     5281 taagccacca gtcgtgaatg gcaataaaat taaaaccgcc aacatcaaaa attctattga
     5341 gtaggtagac actactctga tttatttcgg ttgatgtcag ctgccaagta gcacttagca
     5401 ctagccacat gcttaacaca aatggaagcg ataggtaagg taagtagtac tttcctaaaa
     5461 ctccctgtag tgtaatatta acaagtaatg taaaaaatcc ggcaatggca gccaaaagca
     5521 gaagtgtaat gctaatatca tagtaaaatc ctaacccaaa tccaaccagt aaagcattaa
     5581 aaccatataa gcccttagcg gttgtttccc tatctagttt tatcagataa gatgtaagtg
     5641 tggctactaa tgctgaaacc aacccagcaa taccaatcga aaaatcgata aacgttatga
     5701 gtaacaaaag aatggctaaa gagtattcgc ttgaaaaaaa gatttgcgag taactcctga
     5761 ttagtccctt aatgattgta tttaatccgt ttattgctct cataaacaag tatgctgatg
     5821 gtttacttta ggtgatctgg aatattctca tttgaaacaa tgctatcaat gctctcagct
     5881 ttacggataa tatgtgtttt tcggtttgta tcaattagta taacgttagg cctggtggta
     5941 ataaactgta accactgggt gttattgtag gcacccgttc gggatatcaa aaagtgctca
     6001 cctgttttca agggaggaaa cataattgat gggcgcaata catcaatatt catacagaga
     6061 gggccatata ttacggtttc ctcaactaag cccgaatgag actgtgtggg atttatcttg
     6121 tggtcgtacc aaaacgatgt aaaaagtaag ttaacaccaa catctacaat taaggccctg
     6181 cgtccatcgg taagccgttt atttgctaaa actgtacctg ctatacttcc tgcatcgtca
     6241 atcaatgctc tccctgtttc aagaattaca gtgggtaatt tatcgggttt aatttgtgag
     6301 ttgataattg ctgaggttat ggcatcagca tactcatcga acgagggggt tgtatcttca
     6361 ccagaaaggt atgcacctcg cagggtgtta cgcgatgcaa aaccaccacc catatcgata
     6421 tatgctatgt aatggttaaa cttttgcgca atactggccg ctagttccga cagctttgca
     6481 gcagcaatgc ggtatgcatc ggttgtcatt atatatgttc cgatatgggt gtgcaaaccc
     6541 attaacttaa gctggggatt tgccattatt ctgttaattg cgtcccaagc ctcgccattt
     6601 tcatagttaa acccaaacct atcccatttg ggataaatac ctacatccat gttgacccta
     6661 atagcaaccc gggcattaac actattggct tgacatacct tggcaagaag gaaaagctca
     6721 tcgaaatgat caatgtgaat gtaagcaccc tccttagctg cccttaaaag ttcggcctct
     6781 gtttttcctg ggccgttaaa aataatatgt tcaccgggaa tgccattgag tcgagccttt
     6841 tcgtattcaa atcccgaaac cacttcggcc catgatcctt cctgatgaaa aaccctgcaa
     6901 atggcatcca ggtagttagt tttatagctc catgcaattt gcacttttgg gtaacgcacg
     6961 ctaaatgcgc gttttaaatc attaacattt ttgcgaatta ccgattccga aaagatataa
     7021 agtggggagc catacttttc aattagttcg tcaactttta taccatcaat aaattgcaca
     7081 ggtgctactg caatgcctac gccatgcttt gagggcattc cgggcgttag ctttgtgatg
     7141 gttggacgtt caaaattttt cttttccatt agtttaacct tttagtattc tacaattctc
     7201 cactggttac cagctttgaa aacatgtcaa tatctactat catatcccat gagtaacgga
     7261 taaacatttt cccaatttcg taagacgtgt agggcttaac atctattccc atggcaagct
     7321 ttactaatgc ttcgggtaaa ttttgtccgg caccaacagc aaggtaaacc caagccggaa
     7381 ttcgaggatt tatttcaagg atatatagct tgttatcggc tgtgcgaata agctccagct
     7441 ccatgccacc cctccacttt gtttgtgaaa tgattttatg agttagctca agcattgaat
     7501 cgtccgcgat ggttacccct ccccaggctt tgcctttgtc tgtaatatac tgcttacgca
     7561 tggcaaccgc tgaaatcacc tgcccattcc catcgccaag ggcaattacg ttcacttcgg
     7621 ttccttcaac aaattcctgc acaattacag gataccccca cttagctgac actttcaaaa
     7681 aataggcttg agcctgttcc tgagagtatg ccttgtaggc atcatagtat tttcccttta
     7741 ccatgactgg gtatgtatat gaacgtaaaa catttgcaag gtcattcata ctgtacgcct
     7801 cttcgctttt tggaacattt acgccatact ttttaccgta ctctggcaag cttgatttat
     7861 gccgctcctc aaactgctcc agggtaggaa gaaatgtttt aataccaagc tggttaattt
     7921 gtttttcaag ctttataaac gagaaaagtt cagcatcgaa attgggaata atcagatcga
     7981 tgttttcttt agcattgatt tccttgattc tttcaaataa tgcctcttta ccttttgagg
     8041 ggtaaggaat catgtaggtt ttatccaccc attccttcat gtagattccg ggttctaggt
     8101 gatcgtaagc caaaccaact attctggtat caaagtcagt cgactctttt atgcctctaa
     8161 ttactggcat accaggcccc gggctatcaa tattattgag gccggtaatg gctattgtta
     8221 tttttttacg ctccatggtc ctactcctca agtagtaaat tattccgctt taaatcgcta
     8281 ataaaatcct gtaaatacct ttccaggact aactcgtcaa cttcatactc tgaaagcaac
     8341 ttatccttaa tttcatcttg cgattttccg gattttagca ttgttaaaat ttgaaagccg
     8401 gaatcgttaa gtgtgaatgt gtctcctgta attgggttga atataaaccc gttctcgctt
     8461 atggctatgc tttgatttac tttcatggca aggagtttta tgttaaatta ctatattttt
     8521 tgttacaacc cattttaaaa ataccctact ttaaaaatta atatttttca atttggcttt
     8581 aatacactat ttttgaatgt cttaaaatat tccaacgcat tgaagatatg aagtggttga
     8641 aaataatagc cttactccca tttttctttt catcagtttc atcgtggtgt caggtagata
     8701 gcatcggagc aaaaagtgga ggcatgcaga gagctatggc tgactcatta ctaaatgcaa
     8761 aaagctacac taaggcaatc cctaaataca aggaactact tgtccaattc ccccgcgatc
     8821 ctgaaataaa gtatgcattg ggtgtatgtt acctttacgg gacaagagat gtagagatgg
     8881 cgctgaaata cctgaaggat gcatcaactt ccgatctcaa tacaatggtt tacttttatc
     8941 ttgccgaggc cttacgacag tcgtaccagt ttaacgaagc cattgactac taccgccgat
     9001 ttgttgtaag cggaggatca cccgacatta aactcgatga agttgaaaaa cttgtaactg
     9061 catgtgagaa cggctccttc ctcacccgtt acgtttatag ccctactgtt ttcgaccgaa
     9121 aacaggctag ccccagtaac tttctgaact actactcatc gctattgccc gaaggttctt
     9181 ttactccagt ccccgaaaac cttcgcacag tcaccgacaa gcaaatgggt tacacctctg
     9241 taatgtttta ccccaagaat attaaacccg gcgattacct ttactactcc agctatggca
     9301 agtccacatc gtggggtacc gatattttcc gcattcagct acttgataac gggaagtgga
     9361 gcaagcccga gaatttaggc gatattatta attcctcgtt aaatgaggag tacccattta
     9421 ttatgcccga tggcgttacc ctttactttg catcgaaggg acattacgga atgggtggct
     9481 acgatattta caaatcggtt tataacgccg ataaaaagca gtggagtacc cctgagaatt
     9541 taggctttcc ggtaaattca acgtacgatg attttttatt cctaactagt aacaacgaca
     9601 cacttgcatg ctttacctcg aaccgactaa gcatgcccga tacattaaat gttttcctta
     9661 tccgaaacga catcaaccct acccgacgca ctccaaacag ctacaaggaa ttgctctcca
     9721 tagcaaggct taatcctgtt gagtcaaaac ctagtgcctc acctaaagta cccagttcaa
     9781 aagtagaaac cgataaacct aaggctgaag ccgttgagaa taaaaaacca gcaaagttca
     9841 actccgtaga aaacgaccct gagtatagtc gtgttttagc caaaggcttt gctgcacaaa
     9901 aacttgccga ttcgcttaaa attaagctag aggacttgcg aggccgtttc gattatgtaa
     9961 ctacagccga gcaacgggtt aggctcgaaa agcaggtaac caaggttgag gatgatatgc
    10021 ttgctgccca aaaggaggcc gatttgcttt ttgcccgtgc cagccaaatt gagcaggaat
    10081 accttaccgg taaacgcaaa ccaaaggacg aaagcaacac ctcgtttgtt gccgataacc
    10141 ccgattttat ctatcaggca cagtttgcct caaccgtttt ccgcaccgac gaaatcaata
    10201 gacttgccga cgccgaaaag ttaacaccac agcttatccg tttcagaaat tttgcccttg
    10261 agaggaggga tgcctacaat agatgcctct cagctaatga caacaataat gcttgcacag
    10321 ctgaatataa tcagctggtt gcagccatga aacaatactc cgaccatttt aaaaagtatt
    10381 gggataaaaa atatcaaatt tactccgatt gcattgaagt agcccgtgta aaaagtggta
    10441 accgcgatac cgaagttcga aagctaacca ccgatgccca aaacaacttt agggccgcaa
    10501 tggccattct taacaacctc gattcaaccg gccaggttga aagcgttttt gaggcttcac
    10561 ttttaaggga cttagggtta atgcagtttg accttgcctt tgcaaaaatt tggcgattac
    10621 gccttatgga acaacagctg ctaagcaatg tcattaagct tgaaagaaat atttttggcc
    10681 aatcaagcct aaacctagct caggaggaac aaaaacctac caagaccgtg gaagaggttg
    10741 aagtagacat gcctaagcta aaaagtgaac cggccattaa gcaggccaca ccaaccattg
    10801 ccataattga cgaaataccc gctgactttg gcgttaccga taaaccctac tattccgacg
    10861 aaaagccaat acccattgat gaaccacttc ccagtggggt aatctatcgt attcagattg
    10921 gcgctttcag cgccccacaa aaaccttcat tctttaaggg tatggttcct gtttacggat
    10981 ataaaaccgg aaacatcatt cgctactaca taggtaacct tacccgctac tccgatgcgg
    11041 aaaaggcgct tgttacagtt aagcaaaagg gtttcaaaga tgcttttatc gtggcctggc
    11101 tcaacgggac acgtattaca acccaacgcg ctcagcaact tgaggacgca gtacagcagg
    11161 ttcaacagtc aaagactgac agccgcttgt acatagtaga agtaggacga tacaaaaccc
    11221 ctttaaccga ctcggaaatc aatactgttc gaaacctctc gcagggaaag gagctaaccc
    11281 gtaaggttga ttccaatggc gatcacattt acatcattgg aaattttaac agtatcgatg
    11341 aggccaaccg cgtaaaggaa aatctgattg caagcggaat gctcaaggcg gttgttattg
    11401 atattacacc ataaaatagc tatattagcc taataaatgt ttacgtgata tggcagttaa
    11461 ggaaaaggtt caaggtaaag gaaaacttct gccttcggca gaacttgagt acattttaat
    11521 ccttcacaac gacgatgtta atacctttga ttttgtaatc gataccttgg ttgaggtttg
    11581 caaccacgat cctcatcagg cagagcaatg cgcccttatt acccatttca aaggcaagtg
    11641 cgatattaag catggcgaat ttgaaatgct tgagggtatg atgaacgagt tgcatcgccg
    11701 agggcttagc gctaccattg catcaaacta atagctaaac tatgttctgg attattgcac
    11761 ttatcatttt aggtctgttt ttgctggtgg tagaattctt tatgattccc ggtgttacag
    11821 ttgctggcat tggaggtttt ttaatcatta tatacgctgt ttactctgcc tacgccgacc
    11881 atggaaccac agcaggacat ttaaccctcc tttttacagc aattgcatca attgcaactc
    11941 ttgttttttc attaagggca agaacttgga aacgtgtttc gctcgaaaca agtatcagtt
    12001 caaaagctgt tgaagagctg gagaacgaaa tacatgtggg cgatgaaggg gttacaatca
    12061 gccgtcttgc accaatgggc aaagcctcca ttaagggtaa aaccgtcgag gcctcaacca
    12121 taggtgaact tattaaccca aacacaccca ttgttgtagt acatgttgaa actaataaaa
    12181 ttattgtcaa acctaaataa ctctaaacta tgaatgaagt tggtgcacta cttctgatta
    12241 ttgccctgag cgttgtaggc ttatggataa tcttttactt tgtacctctt ggactttggt
    12301 tccaagcgct ggtatcggga gttaggatat cgctcctgca acttattttt atgcgctggc
    12361 gtaaggttcc gccacgggta atagtacagg ccatgattga ggggacaaag gcaggcctta
    12421 cccttaaccg taacgagatg gaggctcact tcctggctgg tggtcgggtt tccaaagttg
    12481 ttcatgcctt ggtatcggca caaaaggcca atatccccct cgattttaaa atggcaactg
    12541 ccattgacct tgccggccgc gatgtatttg aagctgttca gatgtcggta aacccaaaag
    12601 tgattaatac acccccagta acagccgtag caaaggatgg tattcagctc attaccaaag
    12661 cccgtgttac cgtgagggca aacatcaagc agctggttgg tggagctggt gaggaaaccg
    12721 ttttggcccg tgttggcgag ggtattgtta ccgccatagg ttcatcgctt agccacaagg
    12781 aagtgcttga aaaccccgat aaaatatcca aaaccgttct cgataagggg ttagatgcag
    12841 gaaccgcttt cgaaatcctt tcgattgata ttgccgatat tgatataggc cgtaacattg
    12901 gagccgtact gcaaatggat caagctaacg ccgataaaaa tattgcacag gctaaggccg
    12961 aggagcgcag ggcaatggca gtggcccttg agcaggaaat gaaagccaaa gcccaggaag
    13021 cccgtgcaaa ggtaattgaa gctgaagctg agattcctaa agctattgcc gaggcattcc
    13081 gtagcggtaa ccttggtgtg atggactact atcgtattaa gaatattgag gccgacacca
    13141 acatgcgcga taacattgcc aaatcgggca acgagcctaa aaagaaataa ctataagtaa
    13201 actgataatc aatagaagag agggagcaat ccctcttttt tttgtaatta ttttgcacat
    13261 aaaataagat tttatacttt tgcgttgctt ttagcggaaa caaggctgat taagtaagcc
    13321 cggagaggtg ggtgagtggc ttaaaccaac ggtttgctaa accgtcgtac tccgcaaggg
    13381 gtaccggggg ttcgaatccc cccctctccg ctgaaagttt aacgttcggg gtgtggcgca
    13441 gttggttagc gcacctgctt tgggagcagg gggtcgtgcg ttcgagtcgc accaccccga
    13501 ctggtgacaa ggaagcaaac cgcttccttt ttttgttttt cccatgctgg ttagcgcatc
    13561 ccgcctgtgg gcgggagggt cgacctttca actcgcacca ccccgaccaa gataaagaag
    13621 gaggcagtca agcctccttt tttttttgtg agttcttctc cctcattcat tttagccaaa
    13681 ttctagtatt tcagttccct taagctttgt cttttgcctc tctcttcaac caacaactat
    13741 caaccatcaa ctaacaacta atcaatcagt tggttagcgc atcccgcatg tgggcgggag
    13801 ggtcgaccgt tcgagtcgca ccaccccgac tggtgacaag gaaacaaacc gcttcctttt
    13861 tttatttttc ccatgttggt tagcgcatcc cgcctgtggg cgggagggtc gtgcgttcga
    13921 gtcgcaccac cccgactggt gacaaggaag caaaccgctt ccttttttca tttttcccat
    13981 gctggtttgc gcatcccgcc tgtgggcggg agggtcgacc aatttcgtta ttcgtgattc
    14041 gttactcgtt gttcgggagt aatggaaaac tgatggcact gattaagcag gtttagtctg
    14101 aatattactc attattggct actttttaat tgatagttta atatcaccaa tctatgtcga
    14161 caaataataa aacaggcttt ccttttgtca tactgagcga agcgaaggat atattgaata
    14221 gtgtgcagtg tatagtgttc agtgtacagg gaagatggag agtggtgttt ggtttttagt
    14281 gtttcgtgaa atgctttttt tcactatcaa ctatcaactc ttcttgtcat cctgaacgca
    14341 gcgaaggatc tctaatcgac cctaatgaga tgtttcgcta gtgctcaaca tgacaata
//
LOCUS       DAOSAL010000070        13600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_3063_length_13600_cov_43.521742, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000070 DAOSAL010000000
VERSION     DAOSAL010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 13600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13600
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3063_length_13600_cov_43.521742"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..254)
                     /locus_tag="PK049_09310"
     tRNA            complement(182..254)
                     /locus_tag="PK049_09310"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(219..221),aa:Met,seq:cat)
     gene            400..1839
                     /locus_tag="PK049_09315"
     CDS             400..1839
                     /locus_tag="PK049_09315"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOK61796.1"
                     /translation="MRKLFATNLIFLLLLNVVVKPLWIFAIDRNVQIVLGAQEYGFYF
                     ALFNFSLLFNFLLDLGITNFNNREISYNPQLISQQFANVLVIKLVLALVYFMVTLGIG
                     VSLGFGKRHIVLLAVLTLNQFLASLLLFIRSNLNGLHLFKADSVLSVMDKAIVIVLVS
                     LLLWGNLNIQLTVEVFALSQTLAYVVAIFIAIWLLQGKYSIAIYREFDRITIVTLIKK
                     GLPFAALVLLMTLYSRLDTVMVERLCPEGAVSAGIYAQAFRLFDAFNMYSYLFAALML
                     PIFSRMMSNQEDITELFNFSFNLILIPVVAIAVPTMIYNRPVMSVLYHEHIDVSSKAL
                     VLLMGSFVFVALSYLFGTALTAKGKIWLLCRISLGAVIVTLFSMLILIPRFGVIGAAM
                     ANLVANAIASLLQAFFFIRSQGSAISKTSVLKISLYILIYSAFGLMIQYSVGNNLTGF
                     IATLASSLLVVFGLRLFSFRAIINFARGI"
     gene            1941..2450
                     /locus_tag="PK049_09320"
     CDS             1941..2450
                     /locus_tag="PK049_09320"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOK61797.1"
                     /translation="MGILIEKTQDCPYVNFSDEGILEIEGRSITEDPFTFWQPLLEWV
                     EGYCQKPSPNTQVIIFLEYSNSSSNKYISEIFRKLEEIHGVKTQVSVKWRHEIEDDAI
                     LQLGHDFASIFELPFEFIGVDVEKERFKKVKIRSKKTGTEAIISYRYWDAIVRNGHGD
                     EYQILQEFS"
     gene            2500..2886
                     /locus_tag="PK049_09325"
     CDS             2500..2886
                     /locus_tag="PK049_09325"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOK61798.1"
                     /translation="MQKYELRETATTPYVNFDPDTGVIRMEGRSIPENVIDFYQPILR
                     WIDEYATNPQPETIVHLKFEYFNTSSSKRIFDIMKKFEKLAVDSGKKVTINWYFEEDD
                     EDIYFAGNDYKALINKVDFNLVEIKG"
     gene            2940..4199
                     /locus_tag="PK049_09330"
     CDS             2940..4199
                     /locus_tag="PK049_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOK61799.1"
                     /translation="MNGTNKKKIVNDPVHGFISISHPLIFDLIEHPYFQRLRNIKQLG
                     LSYLVYPGAVHSRFQHCIGAMHLLDLAIETLRSKGLDISDNEAEAAAVAILLHDIGHG
                     PFSHALEHVIANDISHEHLSRCFLYQLNKEFDGKLSLAIEIFEDKYPRHFLHQLISGQ
                     LDMDRMDYLKRDSFYTGVSEGIIGTERIIKMLNVVDDTLVVEAKGIYSIEKFLIARRL
                     MYWQVYLHKTVVAAEQVLIKLLERAKELVLQGQDIYCNEPLLFLLNSKLTKADFFPNP
                     QMPEAPLPLQHFAYIDDNDVMVAAKQWIHHGDKTLSQLANMIVNRRLPSVELNSTGFD
                     SQRLSRLMAEFRKQYIDLADFSDYFVFTDTISINAYKPGEEAIQILYNNGKLVDIAIA
                     SDMLNAKALSDETKKFFLCYPKQLRGI"
     gene            4242..5288
                     /gene="lpxD"
                     /locus_tag="PK049_09335"
     CDS             4242..5288
                     /gene="lpxD"
                     /locus_tag="PK049_09335"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOK61800.1"
                     /translation="MEFTAKLIADFLGGSVDGDPNVKVHTVAKIEEGFPGSLAFLANP
                     KYIPYIYTTKASIVLVNNDFVPDQPVEATLVRVNNAYEAFASLLNLYAQSKQPKSGIE
                     EPSFIHKSAQIGEDPYIGAFSYISEGAVLGNNVKVYPQVYIGPKVKVGDNTILYPGVV
                     IYEECVIGSNCIIHSGAIIGADGFGFASSEETNYKKIPQIGNVIIEDYVEIGANATVD
                     RATMGSTILRKGVKIDDHVHIAHNVEVGENTVMAAQTGVAGSTKIGKNCMFGGQVGVS
                     PHVKIADGVKAGAQAGISGDIRKPGVIVLGSPANEIGKQRRSMAVYKNLPELMNKIHE
                     LEQVVEELRSKLNK"
     gene            5347..6744
                     /locus_tag="PK049_09340"
     CDS             5347..6744
                     /locus_tag="PK049_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HOK61801.1"
                     /translation="MADKQRTIAQAVTLTGKGLHTGAEVEVSILPAEVNTGIIFQRTD
                     MDGQPKIEAVADNVIDTSRGTTIAVKNAKVSTIEHMMAALYSMGIDNALVQVNGPEMP
                     IMDGSSKYFVEAIAKVGTVEQGAERNYLCIKEKMVYTDEKNNIEIIIYPDDKLSIDVM
                     VDYNSRVLGHQYAKMNSIDEFPEQISPCRTFVFFHELEVLLKHNLIKGGDLENAIVVM
                     EHKVPQEELDRIADLFNKPHIAVKPEGILNNLDLHFDNEPARHKLLDMVGDFALVGAR
                     IKGKIIAMRPGHKANTEFAKILRKHYKQERLKPVAPDYDPNKTPLMDVNQIMKILPHR
                     PPFLLIDKILYMDGTTVVGMKNVTMNEAFFVGHFPDEPVMPGVLQVEAMAQTGGILVL
                     NTVPDPENYLTYFLKIDQVRFKRKVVPGDTILFKCFLNEPIRRGIANMTGQAFVGDQL
                     VMEGILMAQITKVKE"
     gene            6744..7553
                     /gene="lpxA"
                     /locus_tag="PK049_09345"
     CDS             6744..7553
                     /gene="lpxA"
                     /locus_tag="PK049_09345"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOK61802.1"
                     /translation="MTQHSNAFIHPDAKIGKDVTIGPFTYIAGDVVISDGTWIGPNVT
                     IMDGARIGKNCRIFPGAVISAIPQDLKFKGEITTAEIGDNTTIRECATVNRGTASRGK
                     TVVGSNCLLMAYSHIAHDCILKNNIIIGNGTQIAGEVEIDDHAILSAHVLVHQFERIG
                     AHVMVQGGSKVNKDIPPFIIAAREPLTFAGINLVGLRRRGFTAQQIGTIQELYRNIYN
                     KGMNVSQALAYIEENMEATPEREIVTSFIKDSKRGIVREYTGSNPDEGVEL"
     gene            7642..8871
                     /locus_tag="PK049_09350"
     CDS             7642..8871
                     /locus_tag="PK049_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HOK61803.1"
                     /translation="MVFLYNLSIRFYQLLVFVASAFNTKARHFYRGRVGIWEKLKSQI
                     EPNSEIIWVHCSSLGEFEQGRPVIEAIEQKKPNAKILLTFFSPSGYEVRKNYSGADYI
                     FYLPLDTYSNASRFIKMVNPAMAIFIKYEFWYHYLNELRKKIIPTYLVSGIFRPDQVF
                     FKPYGAWYRKFLKAFTYFFVQNQQSKDLLASIGYTNVMVTGDTRFDRVSRIASDAKSI
                     PIVENFASNHKVLVAGSTWPKDEDIILPLVTKFPDLKLVVVPHEVTEAHIKDIMGKSS
                     VPAVRFTKSNPEEVKQAKILIVDTIGLLSSIYRYGHVAYIGGGFGVGIHNTLEAAVYG
                     IPVIFGPNFQKFQEAKDLIACKAGFSIKTYDELVDLLNLLLFDKNFYQLSASEAKQMV
                     NSGVGATQKILEVITAP"
     gene            9002..11551
                     /locus_tag="PK049_09355"
     CDS             9002..11551
                     /locus_tag="PK049_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013760737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOK61804.1"
                     /translation="MKRTIWCTLLMLLFLAPMLTLGQGVIKGIIVDSNNGNPLIGAAV
                     VVEGTTIGTSTSVDGSFNFKVPIGKHVIKFSYVGYLDFEKNVEVTGDVDLGTIKLSPS
                     LVDIEEVLVTASFVRDRTTPVAVSTIEPKVIVEKLGNKEFPEILKITPSVYATRTGGG
                     FGDSRIYLRGFDSNNIGVLINGIPVNDMENGKVYWSNWAGLSDVTANQQVQRGLGASK
                     LALSSVGGTINIITKSTDAQKGGSYYYGFGNDGFRKQNFTVSTGLMENGWAVTVSGGY
                     TTGNGYVQATNFEGYSYFANISKKINEKHTITFNAFGAPQWHNQRNNKHTIQEFRDNP
                     NGIKWNSDFGYRNGQIYTTGYAYNFYHKPQMSLNHYWKLNDNSMLTTQIYASLATGGG
                     RRVYGQNSTWLSRQFPSGLPYDVTALTQEGYYDYDWVIQQNAAAMNGSTCIIANAVND
                     HQWYGALSTFTTNIESLKLTAGFDGRFYNGVHAYKIDDLLGGKYFLDNKNINRPASQP
                     LYKGDYVNYHYLGQVLWLGLFAQGEYIKDNYSGFLSMAIASNNYRRVDYFQYEPGNRA
                     SAWHDFLPWNVKAGFNYKVYGEHNVFVNGGYIKRAPIFSNSFLNNTNTFNDGVKYERI
                     ITAELGYNYASRIFNVKVNLFHTQWLDKGLVKSLSGQTANILGINALHQGVEIEGIVK
                     PISKLDIRGMFSWGDYRWSDDVNFKLYNENQELIGTYNAYIKNVHVGNSAQVSAAVSC
                     NYEILPKLKIGADVNWYAKNYADFDPTTRTTPDSKQDAWRMPNYYTIDLDANYRFKIG
                     NLNATLYGNVNNLLNTEYIADATDGTNHDEFTSYVFFGFGRTWSTGLRINF"
     gene            11570..13531
                     /locus_tag="PK049_09360"
     CDS             11570..13531
                     /locus_tag="PK049_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61805.1"
                     /translation="MKKILAILLGVSLLWACDPMEDTYQKLDDAKQPYKEVINYTLIS
                     TDYTSASNLALKDALTHDDSTLAKAIKSQLAFNERFAGKDYIPDILTKNFPALSKGSS
                     ANIQYDVQYNTPVNILDLYSNNIISEQEYKDSVWKSSTLFVKSFTPSTIGRMPQVLLK
                     KFEGASTNAYRLVEYNYSEVEPTIVDNAEFKYFEENFETHTCSTSSPYTPIGENGWTQ
                     KDTVTGSVKGIYYCRKYNNNQYAQITSYNTGEKNDVFLITKEIDLTDANSPILTFDVN
                     VGNWNATCLSILISSNYTGDLNQIGTATWVDLTSNFTLPETPTSGYGTFANAGQADIS
                     AFKGKKVYIAFLYKGDSRTTANPKVTTTYQIDNVKVSEIKSSFVLSETNIKKYWKVYK
                     FNGTSWATAENKYIAIQADDYALMGKTYITSSESDAYVIPFLKLKYPFALEGDAKVVS
                     FRSESNQTKALAYEYKYSNGNWTKNSYRETRNDQFLNDGTKWFYDPTVYFTPVSADYQ
                     LLVNWVYQNLDRSYGSSYGNDEFYYGASAYYSNWDLRLSKRTQYNIPGFDTGTESEKI
                     ALTWQRLEEGAAKLLSLKFPDAVSEISGFPVYYWIYLKVYQNDLKTVTYVGVFQLQSG
                     EFKRVKTAEDAAVSQGKLTQDQVNWNRSN"
BASE COUNT         4100 a         2543 c         2880 g         4077 t
ORIGIN      
        1 aaaaccccgc cattggcggg gcttacttct ttgtagcggg ggcagagcca gtcccgccaa
       61 gcgggaactc gaacctgcga gtgaataaat taacaggagt ttatgcggta aaaattggaa
      121 ttatcggtta agtagggaat taaaacataa aaccccgcca ttggcggggt ttacttcttt
      181 gtagcggggg caggactcga acctgcgacc tttgggttat gagcccaacg agctaccaac
      241 tgctccaccc cgcaattcag tttgcgagtg caaatatagt aaagttcacg taaacaacaa
      301 aacacaatta atttttttta acaaaaacag atgtttttga gctttgcata tcgcattttg
      361 ctacatttaa aatatcttgc gctactatac ttgctataaa tgagaaaatt atttgccacc
      421 aatttaattt tccttttgct tttaaatgtg gtagtgaagc cgctttggat atttgccatt
      481 gaccgcaatg tgcaaattgt acttggtgct caggagtatg gtttttactt tgccctcttt
      541 aatttctcac ttcttttcaa ttttttactc gatcttggta taaccaattt caataaccgc
      601 gaaatcagct ataacccaca gctgatatcg caacagtttg caaatgtgtt ggtaataaag
      661 ttggtgttgg cattggtgta ttttatggtt acattgggta ttggggtttc attgggtttt
      721 gggaaaaggc atatagtcct tttagccgtg cttacactta accaattttt agcatcgcta
      781 ttacttttta tccgatcaaa ccttaatggc ttacatcttt ttaaggccga tagcgtgtta
      841 agtgttatgg ataaagcaat tgttatagtt ttggtatcgt tacttttatg gggaaacctt
      901 aatatacaat taacagttga ggtatttgca ctatcacaaa ccctagctta tgtagttgca
      961 atatttattg caatttggtt gcttcaaggg aaatatagca ttgcaattta cagggagttt
     1021 gacaggatta ctatagtaac gctgattaaa aagggcttgc catttgccgc gctggttctt
     1081 cttatgacac tctacagtag gctcgataca gttatggtgg aacgtttatg ccctgaggga
     1141 gcagtttcgg caggcattta tgctcaggca tttaggttat ttgatgcatt taacatgtac
     1201 tcatacctgt ttgctgcgct tatgcttcca attttctcgc gcatgatgag taaccaggaa
     1261 gatattacgg aattatttaa cttttcgttt aacctgattc taattcctgt ggttgccatt
     1321 gcagttccaa ctatgattta taaccgcccg gtaatgagcg ttttatacca tgagcatatc
     1381 gatgtttcat ctaaagcatt ggttttacta atgggctctt ttgtttttgt ggcattatca
     1441 tatcttttcg gaactgctct aacagccaag gggaaaattt ggttgctttg tagaatatcg
     1501 cttggtgctg taattgtaac ccttttttcc atgctaatcc ttataccgcg ttttggggtg
     1561 attggtgcag ccatggctaa ccttgttgct aatgcaatag catctttact gcaggctttt
     1621 ttctttattc gtagtcaagg gagtgcaatt agcaaaacat cggtgctaaa aatatcacta
     1681 tacattttaa tttactcggc ttttggtttg atgattcaat actccgttgg taataactta
     1741 acgggattca tagcaactct tgcatcaagt ttacttgtag tttttggact aaggttattt
     1801 tcctttaggg ctattattaa ttttgctagg ggtatttaac ctgagaaaag ttgatgttca
     1861 taaaaatgca tttgttttag atcttttttt ttaatacttt tgacaatagt ccttaattgg
     1921 catggtaaaa taccatagga atgggaatac ttattgaaaa aacacaggat tgtccgtacg
     1981 taaatttttc cgatgaggga attctcgaaa tagaaggtcg ttcaattacc gaagatcctt
     2041 ttaccttttg gcaacccctt ttggagtggg ttgaaggtta ttgccaaaaa ccttcgccaa
     2101 atacccaagt gattatcttt ttagagtatt ctaacagtag ctcaaataag tacataagtg
     2161 agatttttcg taaactcgaa gagattcatg gcgtcaaaac acaggtatca gtaaaatggc
     2221 ggcacgaaat tgaggacgat gccattcttc agttaggtca cgattttgcg tcaatatttg
     2281 aactgccttt tgagtttatt ggggttgatg tggaaaagga acgcttcaaa aaggtcaaaa
     2341 tccggagtaa gaaaacgggc actgaggcca taatttcgta taggtactgg gatgcaatag
     2401 ttagaaatgg ccatggcgat gagtaccaaa ttttacagga attttcatag tatctaattt
     2461 ttttgcatat ttgttttttt agtgataaaa cttttggtta tgcaaaagta cgagttgaga
     2521 gaaacggcaa caacaccata cgtgaacttc gatcccgata ccggtgttat tcgcatggag
     2581 ggacgatcga ttcctgaaaa tgtaatcgac ttttaccaac ccattctgcg atggattgat
     2641 gaatatgcaa ctaatcctca acccgaaaca attgtacacc tgaagtttga gtactttaac
     2701 accagctcct caaaacgtat ttttgatatc atgaaaaagt ttgagaagct ggcagttgac
     2761 tctgggaaaa aggttaccat taactggtat tttgaggagg atgatgagga tatttacttt
     2821 gctgggaatg actataaagc tttaataaac aaggtggatt ttaatttggt tgagattaaa
     2881 ggctaatcct ttttgagatg gtatagcaat atatcacccc tctggggtga tagttttata
     2941 tgaatgggac taataagaaa aaaattgtaa acgatccggt tcatggcttt atcagcataa
     3001 gccacccatt aatttttgac cttattgaac atccctactt ccagcgtttg cgaaacataa
     3061 agcagctcgg actgagttac ctggtatatc ccggcgccgt tcattcccga tttcagcact
     3121 gcattggtgc aatgcaccta ctcgaccttg ctattgaaac actccgaagc aaggggctcg
     3181 acatttccga taacgaggcc gaagctgctg ccgtagcaat tctgctccac gacattggtc
     3241 acgggccttt ctcacatgcc ctggagcatg taattgctaa cgatatcagc catgaacact
     3301 taagccgatg ctttttgtac cagctcaaca aggagtttga tggcaaactt tcacttgcca
     3361 ttgaaatatt cgaagataag tacccacgcc acttccttca ccagcttatt tcaggacagc
     3421 tggatatgga tcgtatggat taccttaagc gcgatagctt ttacactggt gtatccgagg
     3481 ggataattgg tacggaacgt atcattaaga tgttaaatgt ggttgacgac accttggtgg
     3541 tggaagcaaa gggtatatat tcaattgaaa aattcctgat tgcccgtagg cttatgtact
     3601 ggcaggtata ccttcataaa acggttgttg ctgctgagca ggtgctaatc aagttgctgg
     3661 aaagggctaa agaactagtg cttcagggtc aagatattta ctgtaacgag cctttactat
     3721 tcttgctcaa tagcaagcta accaaagccg attttttccc aaacccccaa atgccggagg
     3781 ctccgcttcc acttcagcac tttgcctaca ttgatgacaa tgatgttatg gttgctgcaa
     3841 agcagtggat acaccatggc gataagaccc tttcacagct tgccaacatg attgttaatc
     3901 gccgtttgcc tagtgttgag ttgaatagca caggctttga tagccaaaga ctaagccggt
     3961 tgatggcaga atttagaaag cagtatattg atctggctga tttctctgat tacttcgttt
     4021 ttactgacac cattagcatt aacgcctata aacctggcga ggaagcaatt caaatacttt
     4081 acaacaacgg aaaactagtt gatattgcca tagcctcgga catgctcaac gctaaagctc
     4141 tttccgacga aaccaaaaaa ttttttcttt gttatcctaa acagcttagg ggcatttaga
     4201 ttaaattaat tagttttgca aattgtttac aattacttaa tatggaattt acggcaaagc
     4261 ttatagctga ctttttggga ggctcagttg atggcgatcc caacgtgaag gtgcatacag
     4321 tggcaaaaat tgaggaggga tttcctgggt cgcttgcatt cctggccaac ccaaaatata
     4381 ttccttacat atacactact aaggcatcaa tagttctggt aaataacgat tttgtccctg
     4441 atcaaccggt tgaggcaacg cttgtcaggg taaacaatgc ctacgaggct tttgcgtcgc
     4501 ttctgaactt gtatgctcag tcaaagcaac ccaagagcgg aattgaggag ccatcattca
     4561 ttcacaagtc ggcccaaatt ggggaagacc cctacatagg agctttcagc tatatttcgg
     4621 aaggggctgt tttgggtaac aatgtgaagg tataccctca ggtttacatt ggcccaaaag
     4681 taaaagttgg cgataatact attctatacc cgggtgtggt gatctacgag gagtgtgtta
     4741 ttggtagcaa ctgtatcatc cacagtggtg ctattattgg tgccgatggt ttcggatttg
     4801 catcgagcga ggaaaccaac tacaagaaaa ttccacagat tgggaatgtg ataattgaag
     4861 attacgtgga gataggcgct aatgccacgg ttgatagggc aaccatgggg tcgacaatac
     4921 ttcgaaaagg ggttaagatt gacgatcatg ttcacattgc acacaatgtt gaggttggcg
     4981 aaaatacggt tatggctgcc caaactggtg ttgcaggatc aaccaagatt ggcaaaaatt
     5041 gtatgtttgg cggacaggtt ggagtgagcc cccatgttaa aattgccgat ggggttaaag
     5101 ctggcgcaca ggctggaatc tcgggcgata tccgaaaacc cggggtaata gttttaggaa
     5161 gcccagccaa tgaaattggc aaacaacgcc gatcaatggc tgtgtataag aatttgcctg
     5221 agctgatgaa taaaattcat gagctggagc aggtagtgga agagttgcga tcaaaactta
     5281 ataaatagca gtatgcaaag gcgctcaaat gcgccttttt gtcgtttaac tgaaatttta
     5341 atcaaaatgg ccgataaaca aagaactata gcacaggcgg ttaccttgac aggtaagggg
     5401 ttacatactg gcgccgaggt agaagtatca atactaccgg ccgaggttaa caccggtatc
     5461 atattccagc gaaccgatat ggatggtcag cctaaaattg aagcggttgc cgataatgtt
     5521 attgacacct caaggggaac caccattgcg gttaaaaatg ccaaggttag caccattgaa
     5581 cacatgatgg ctgcccttta cagcatgggg attgataacg cattggtgca ggttaacgga
     5641 cctgaaatgc cgataatgga tggtagctcc aagtattttg tggaggctat tgccaaggta
     5701 ggaacagttg aacagggtgc tgagcggaac tacctttgca ttaaggagaa aatggtttac
     5761 accgatgaaa aaaacaacat cgaaattatc atttaccccg acgataagct gagcatcgat
     5821 gtgatggtcg attacaactc aagagtgctt ggacaccagt atgccaagat gaactcaatt
     5881 gatgagttcc cggaacaaat atcaccctgc cgtacctttg tattcttcca tgagttagag
     5941 gtgttgctaa aacacaatct tattaaaggg ggtgacctgg agaatgccat tgttgttatg
     6001 gagcacaagg ttccacagga ggagcttgac cggattgccg atctatttaa caagccacac
     6061 attgccgtta aacccgaagg tatccttaat aaccttgacc tacatttcga taatgaaccg
     6121 gcacgccata agctgctcga tatggttggc gactttgccc ttgttggtgc tcgcattaag
     6181 ggaaaaatta ttgccatgcg tccaggtcat aaagcaaaca ccgaatttgc taagattctt
     6241 cggaagcact acaagcagga gcgcctaaaa cctgttgccc ccgattacga tcccaacaag
     6301 actcccttga tggatgtgaa tcagatcatg aaaattcttc cgcatcgtcc tccattccta
     6361 ttaattgaca agattcttta tatggatggt accaccgtgg tgggcatgaa gaatgttacc
     6421 atgaacgagg ccttttttgt gggacatttc cccgatgaac cggtaatgcc aggtgtactt
     6481 caggttgaag caatggcaca aactggtggc attcttgttc ttaatactgt tcccgatcct
     6541 gagaactatt taacttactt cttaaagatt gaccaggttc gtttcaaacg aaaagttgtt
     6601 ccgggcgata caattctttt taaatgcttc ctcaacgaac ccattcgcag gggaattgcc
     6661 aacatgaccg gacaggcttt tgtgggcgat caattggtta tggaaggaat tcttatggct
     6721 caaataacta aagttaaaga ataatgacac agcatagcaa cgcatttatt catcccgatg
     6781 ccaaaattgg caaggatgta accattggac cattcacata cattgcaggc gacgttgtaa
     6841 ttagcgatgg tacatggatt ggaccaaatg ttaccatcat ggatggtgca cgcattggca
     6901 agaactgccg aatatttccg ggagcagtaa tctctgcaat accacaggat ttaaaattca
     6961 aaggtgagat taccactgcc gaaattggcg ataacaccac catccgtgaa tgcgcaactg
     7021 taaaccgggg tacagcatca agggggaaaa cagtggtggg tagtaactgt ctcctaatgg
     7081 catactccca tattgcacat gattgtatcc taaagaataa tatcataatt ggcaatggca
     7141 cccagattgc cggtgaggtt gaaattgacg accatgccat tttaagcgca cacgtgctag
     7201 tgcatcagtt tgagcgtatt ggcgcccatg ttatggttca gggtggatca aaggttaata
     7261 aggacattcc tccttttatt attgcagcac gtgaaccttt aacttttgct ggtattaatc
     7321 tggtaggtct tcgccgtaga gggtttaccg ctcagcaaat tggaacaata caggaactat
     7381 accgaaatat ctataataaa ggtatgaacg taagccaggc attagcttac attgaggaga
     7441 atatggaagc cactcctgag cgcgaaatag taacatcctt tattaaggac tcaaaacggg
     7501 gaattgtgag ggaatatact ggttccaatc ccgatgaggg tgtagaactt taggaatatc
     7561 atgccggctt ttgccggcat tttttttggt acgggtttaa cctttagcta tatttgactg
     7621 aacttataga accaatctta tatggtattt ctttacaacc tgtcaattcg attttatcaa
     7681 ttgctggtct ttgtcgcatc ggcattcaac accaaggcaa ggcattttta taggggcaga
     7741 gttgggatat gggaaaaact taaaagtcaa atagagccta attccgaaat tatttgggta
     7801 cactgttcgt cgttaggcga gtttgagcag ggaagacctg ttattgaggc catagagcag
     7861 aagaaaccta atgctaaaat attgcttact ttcttttccc catcggggta cgaggttcgg
     7921 aaaaactatt ccggagccga ttatatcttc tacttaccac tcgataccta ctccaatgca
     7981 agcaggttca taaaaatggt aaatccggca atggccattt tcattaagta tgagttttgg
     8041 tatcactacc tcaatgagct taggaaaaag attatcccaa cctaccttgt gtcagggatt
     8101 ttccgacccg accaggtgtt ctttaaaccc tatggcgcat ggtatcgcaa gtttctcaaa
     8161 gcattcacat acttttttgt acaaaaccaa caatcaaagg atctgctagc cagcataggg
     8221 tataccaatg ttatggttac tggcgatacc cgattcgata gggtttccag aatagcctct
     8281 gatgcaaaga gtatccctat tgttgaaaac tttgcatcaa accataaagt gcttgtagcc
     8341 ggcagcacat ggcccaaaga tgaggatatt attttacctt tagtaacaaa gttccccgat
     8401 ttgaagttag ttgttgttcc acatgaggta acggaggcgc atataaagga tataatgggt
     8461 aagtcgtcag ttccagctgt tcgatttacc aaatccaacc ccgaagaggt taagcaagca
     8521 aagatactca ttgttgatac aatcggcttg ctatcgtcaa tttataggta tggtcatgtt
     8581 gcctacatag gaggagggtt tggagtgggt atccacaata cccttgaggc tgctgtttac
     8641 ggtattcccg taatattcgg gcccaacttt caaaaattcc aagaggcaaa agatttgatt
     8701 gcatgtaaag caggattttc tatcaaaact tacgatgagc tagtggattt actaaactta
     8761 ctgctcttcg acaaaaattt ctaccagcta tcggcaagtg aagcaaaaca aatggtaaat
     8821 tcaggcgttg gggccactca aaaaattctt gaggttatta ctgctccatg aaacaatttg
     8881 tttcaaacgt ttgcgggggt ttaacaattt gttaatgttt ctttgtgatt tttcggtatt
     8941 atatgaaatt aactttgcat aaatgaatat tagccttaat taactctaaa atcaaaaacc
     9001 tatgaaaaga acgatttggt gtacattgtt aatgctttta ttccttgctc caatgttgac
     9061 tttagggcag ggagtaataa aagggataat tgttgactca aacaatggaa accctttaat
     9121 tggtgctgct gtggtagttg agggcacaac tattggaact tcaacaagcg ttgacggctc
     9181 atttaacttt aaagttccaa ttggaaagca tgtgattaag ttttcttatg tgggctacct
     9241 agactttgaa aaaaatgttg aggtaactgg cgacgttgac cttggaacca ttaagttaag
     9301 ccctagtttg gttgatattg aggaagttct tgttacagca tcgtttgttc gggataggac
     9361 aacccccgtt gctgtttcaa caattgaacc aaaagtaatt gttgaaaagt taggaaacaa
     9421 ggaatttcct gagatactca aaataacacc atcagtatat gctacaagaa caggaggtgg
     9481 ttttggagat tcaagaattt accttcgtgg atttgactca aataacatcg gagttttaat
     9541 aaatgggatt ccagtaaatg acatggaaaa tggtaaggtt tactggagca actgggcagg
     9601 actatccgat gtaaccgcaa atcagcaggt tcaacgtggt ttaggtgctt ccaaattggc
     9661 tttgtcatca gttgggggta ccattaatat tattacaaag tcaactgatg ctcaaaaagg
     9721 aggctcatat tactatggct ttggtaacga tggcttcaga aaacagaatt tcaccgtttc
     9781 aactggttta atggaaaatg gttgggctgt taccgtatcg ggtggttata ctactggaaa
     9841 tggatatgta caagctacta attttgaggg ttactcatat tttgcaaaca tttcgaaaaa
     9901 aattaatgag aaacatacaa taacctttaa tgcatttgga gcccctcagt ggcacaacca
     9961 acgaaataat aagcatacta tccaagagtt ccgtgacaat ccaaatggaa ttaagtggaa
    10021 ttctgacttt ggatatcgca acgggcaaat ctatactaca ggatatgctt acaattttta
    10081 ccataaacct caaatgtcct taaatcacta ctggaagcta aacgataact caatgcttac
    10141 aacacaaatt tatgcatcgt tagctactgg tggtggtcgt agggtttatg ggcaaaattc
    10201 aacatggcta agtcgtcagt tccccagtgg attgccatat gatgtaaccg ctctaaccca
    10261 agaaggatat tatgactatg attgggtaat acagcaaaat gctgcagcaa tgaatggttc
    10321 aacatgtatt atagctaacg ctgtaaatga ccatcagtgg tatggagctt tatcaacctt
    10381 tactactaac attgaaagcc ttaagttaac tgctggtttc gatggacgtt tttataatgg
    10441 tgtacatgcc tataagattg atgatttgct tgggggcaaa tatttcctgg ataataagaa
    10501 tattaatcgt cctgcaagtc aaccgcttta caaaggagat tatgtaaact accattacct
    10561 aggacaggta ctttggttag ggctatttgc tcaaggtgag tatatcaaag ataactactc
    10621 tggtttccta tcgatggcca ttgcaagtaa caactatagg cgagtagatt atttccagta
    10681 tgaacctggt aatcgcgcat cggcatggca tgacttcttg ccttggaatg ttaaagctgg
    10741 tttcaattac aaggtatatg gggagcacaa tgtatttgta aacggtggtt atatcaagag
    10801 agcacctatt tttagcaatt ccttcttgaa taataccaat acatttaacg atggtgtaaa
    10861 gtatgaacgt attattactg ccgagttggg gtataactat gcttcaagaa ttttcaatgt
    10921 aaaagtaaat ctattccata cccagtggct cgataaaggg ttagtaaaaa gtcttagcgg
    10981 gcaaacagca aacatcctag gtattaatgc attgcatcaa ggtgttgaaa ttgagggcat
    11041 tgttaaacca atttccaaac ttgatattag gggtatgttc tcttggggtg attaccgttg
    11101 gtccgatgat gttaatttta agctttataa tgaaaaccaa gaacttattg gcacttataa
    11161 tgcttacatt aaaaacgtac atgtaggaaa ctctgctcag gtttctgccg ctgtttcatg
    11221 taactatgaa attcttccaa agttaaagat tggagctgat gtaaattggt atgctaagaa
    11281 ctacgccgat tttgacccta ctacaagaac aactcccgat tcaaagcaag atgcttggcg
    11341 catgccaaat tattacacca tagatcttga tgctaactat cgtttcaaaa ttggaaatct
    11401 gaatgctact ctttatggca atgtaaataa tttacttaat actgagtaca ttgccgatgc
    11461 tacagatggc accaatcacg atgaatttac ttcatatgta ttctttggtt ttggaagaac
    11521 ctggtcaact ggtttaagaa ttaactttta aaaattggga ggatttataa tgaaaaagat
    11581 attagcaatt ttacttggtg tctcgttgct gtgggcttgt gatcctatgg aggataccta
    11641 tcagaaacta gacgatgcta agcaaccata caaagaagta attaactaca cacttattag
    11701 tacagattat accagtgcta gtaacttagc attaaaggat gcactaaccc atgacgatag
    11761 cactttagct aaagcaatta aatcgcaact ggcatttaat gagcgttttg caggtaagga
    11821 ttatatacct gacatattga caaaaaattt ccctgctcta agcaaggggt cttcagcaaa
    11881 cattcaatat gatgttcaat acaatactcc tgtaaacata cttgatttgt attcaaacaa
    11941 tattattagc gaacaggagt ataaggattc tgtttggaaa agttccacct tatttgttaa
    12001 atctttcacc ccatctacaa ttggaagaat gcctcaagtt ttactaaaaa aatttgaggg
    12061 agcctcgacc aatgcataca gacttgttga gtataattac tccgaagttg aacctactat
    12121 tgttgataat gctgaattta agtattttga agaaaatttc gagactcata cttgtagtac
    12181 atcatcacct tacacaccaa taggtgaaaa tggatggaca caaaaggata ctgttactgg
    12241 tagcgtaaag ggaatatact actgtagaaa atataataat aaccagtatg cccaaattac
    12301 ctcatataat accggtgaga aaaatgatgt atttttgatt accaaagaaa tagatttaac
    12361 cgatgctaat agccctattc ttacctttga tgtaaatgta ggtaactgga atgcaacatg
    12421 cctaagtatt ttaatttctt ctaattacac tggagacctc aatcaaattg gtactgcaac
    12481 atgggttgat ttaacctcaa attttacatt acctgaaacg ccaacgagtg gttacggaac
    12541 atttgcaaat gctggccagg cagatattag tgcttttaaa gggaaaaaag tttacattgc
    12601 ctttttatac aagggcgata gccgtactac tgctaacccc aaagttacta ctacttacca
    12661 aattgataat gttaaggttt ctgagatcaa atcttccttt gtattgagtg aaacaaatat
    12721 taagaagtat tggaaagttt ataagttcaa cggcacaagt tgggccactg ctgaaaataa
    12781 atacattgcc atccaggctg acgattacgc acttatgggt aaaacctata taacatcgag
    12841 cgagtccgat gcttacgtta tccctttcct caagttgaag tatccgtttg ctcttgaagg
    12901 tgatgccaag gtagtatcgt tccgttcgga aagtaaccaa accaaagctc ttgcttatga
    12961 atataagtac tccaatggta attggacaaa aaattcatat cgtgaaacca gaaatgatca
    13021 gttcctaaac gatggcacaa aatggtttta tgatccaact gtttatttta cacccgtatc
    13081 ggccgattat caactcctag ttaactgggt ttatcaaaat ttagatcgta gttatggtag
    13141 tagttatggc aatgatgaat tttactacgg agccagcgct tactattcaa actgggattt
    13201 acgtttatct aaacgtacac aatataatat cccaggattt gatactggta cagaaagtga
    13261 aaagattgca ctaacttggc agcgacttga ggagggtgca gcaaaactac tctctttaaa
    13321 attccctgat gcagtttctg aaatttctgg attcccagta tattactgga tttatttaaa
    13381 ggtttatcag aacgacctaa aaacagttac ctatgttggt gttttccaat tacaatcggg
    13441 tgaattcaaa cgtgtcaaga ctgcggagga tgcagcagtt tcacagggaa agcttactca
    13501 ggatcaggta aactggaata gaagtaatta attttaattg aggcatccaa attgagggcg
    13561 aaggcaactt cgccctcttt tcttttccgc attttatcag
//
LOCUS       DAOSAL010000071        13345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_3140_length_13345_cov_45.726862, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000071 DAOSAL010000000
VERSION     DAOSAL010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 13345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13345
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3140_length_13345_cov_45.726862"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            232..3156
                     /locus_tag="PK049_09365"
     CDS             232..3156
                     /locus_tag="PK049_09365"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOK61806.1"
                     /translation="MKRLLILLTIFALILNLGFAIQKEHVKNDSLSHKSQTEAVHATN
                     MASEIENQGEVTEGSHEGHGLNLAPFLFIILSLFIGTATRHLLSKGLLPYTIMLLIIG
                     LILGLLTRFDILQAVGLGIIGQAVQWAGNIDPHLILYLFLPTLIFEAAYALHIHTFKK
                     SLVNAVILAVPGIMVAIVITAFALLLIKSMGIGLNEWTILLAFLFGAIISATDPVAVV
                     AILKEVGASKRLSTITESESMLNDGTAIVFFMTIFGLITGEAGGNAFVEFLKVSLGGV
                     LVGAVIGWGILSWLKKVFNDALIEITVVIGAAYLSFYIAESVLHVSGVIAVVTLGISM
                     AGPGKTKVSPEVTHFLHEFWELAAFIANTLIFIIVGVVIAQQVNYTLSDLKVLLLVYV
                     TIHIARLGTIYVFYPLMRRIGYGIAFKDSIVLWWGGLRGAVALALALIVAIDSRIPFE
                     PRNQLLSLTAGIVILTSLINATTVRWLIDKLGLSKIGDIKAGLMMQSLQQIRLSGEKE
                     IEKLKENRYMSGADWDKVSSFLIDANAVEEKPQHFNLEDAIAETRKRLLQKEKESYWR
                     QFSMGMLSSEGVNLLSDQIDALLDFGGKIPLSERQDIENIWTTPKTIAKLQNLPLVGR
                     IWKRKFLNRLALSYDCARAFVAAQEENQKSLSSLIIGFSLGGAESSKETELLSALEDE
                     LNENRITGQTFLRNLREKYPDICRSIETLLASRSLLNQQEEMLERLKKQGRLEPDEVE
                     RIQAYIHSSMKQLIDTSLKENTELKVAAALRNTPGLNNLSKDAQQFLITKGQLKIYPE
                     GSVIAKQDSEPDGIYIIINGTVNYYKSDTLTGVKEISDVFGAYEWFISSKLRYTFTAE
                     TPVTLIKINTSTIKLLTSKFPETNRLLQFCASADIALELLKQNEKFSEQSIRKLRNLI
                     RNAELFELNDGKKHRFSGSACILVKGQAITEEQDTLFTAPTILEECNFRSNGDALALI
                     LP"
     gene            complement(3153..3617)
                     /locus_tag="PK049_09370"
     CDS             complement(3153..3617)
                     /locus_tag="PK049_09370"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HOK61807.1"
                     /translation="MRLFCFSALLILFATTTQAQGQTPVSNYNKALADSLGADEYGMK
                     WYVMVMLKTGTKQIKSRDSLQWLYKGHIDAINEMTNSGTLIVTGPMGKNDKNFRSFYI
                     LNTSKTEEAQKLMNANPAVKFGLFDAEYFFWYGPAALPTYKRFQPMVEAKKP"
     gene            3766..4911
                     /locus_tag="PK049_09375"
     CDS             3766..4911
                     /locus_tag="PK049_09375"
                     /inference="COORDINATES: protein motif:HMM:NF017495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HOK61808.1"
                     /translation="MAQILLVLFYLLIPFVILHLCHKFPFVNKLGAVFIAYLVGLIVG
                     NIGILPEWAGGIQDTLTTITVPLAIPLLLFSSNLKQWKSMAGKAMVSLLIGITSVVAI
                     VIAGYFIFMGKGMADLWKVSGMLVGVYTGGTPNLASLKMMLNVDPDVYILTHTYDMII
                     STVFLAFLMTVGKKFFSLFLPAFKDDNMEEVEYTNGSDPFWGIFKREIFYPLLKAYGL
                     TILIFAIAGGLSLLVPKNSQVVVVILTITTLSIVASTFKKINAIEKTFESGMYLILIF
                     SVVVASMADISRFAGLTPGLFGYITLAVFGSLFLQVVLGKIFKVDADTTIITSTALIC
                     SPPFVPVVAAAINNRKVILSGISIGIIGYAVGNYLGFAIAELLKTIQ"
     gene            complement(4953..6302)
                     /locus_tag="PK049_09380"
     CDS             complement(4953..6302)
                     /locus_tag="PK049_09380"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOK61809.1"
                     /translation="MRKLILLAAILFTVTGLFAQQTSDDKKAEKVKSGWNVGGLPVVA
                     YDTDLGFEYGALVNLFHYGDGTIYPKYKHNVYAEVSRFTKGSGINQLFYDSKYVIPGL
                     RVTADLSYLTDKMYDFYGFNGYESVFNLDWADDESADYRSRAFYKYDRKLLRFTTDLQ
                     GKLKNEKFGWVAGIGLYDFKIDTVDVDKLNRGKDEADKLPYVGGGLYQRYINWGLIGE
                     DEKNGGFVTYLKVGAVYDTRDNEPNPMKGMWTEAVITYAPSFLGIGSKYQHAKLSIIH
                     RQYFTLVPERLSFVYRLGYQGTLFGTCPFYLQPNMTTIFLRGATNEGLGGAKSLRGII
                     RNRVVGDGIAYGNFEMRWKFARFHFINQNFYLSLNAFVDGGQVVRKVDVEVPNFIEVP
                     YSPSDYFSNDKEKLHTSWGLGLRVVMNQNFVIVVDHGRAMTKDDGLSGTYIGLNFLF"
     gene            complement(6453..8063)
                     /locus_tag="PK049_09385"
     CDS             complement(6453..8063)
                     /locus_tag="PK049_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HOK61810.1"
                     /translation="MEQEKKKREFPHTYVIIFALIVFAAVLTWFVPGGKFAREVKNVN
                     GIDREVIVPGSFHQVDNQPQTWQVFTSIFQGMNRTYDIIFYILMIGGAFWLLNESKAL
                     DVAILSFLNFTKRIERFKPLKVIGVDNLVIALIMICFSFFGAVIGMSEETIAFVVIFV
                     PLAISMGYDSIVGISLCFLGAGLGFASALLNPFTIGIAQGLSGIQLFSGIEYRFVIWV
                     IINTIGIGYVIWYARRIKKNPKLSPVYEIDEYWRHKAAQDENGTKSKPGKSAWATFIG
                     LALVFAIFAYYYPVSHLVVGQSIISVPIIPIATVVWILVGILALRHSVQLFIVNILLF
                     TILFLIVGVMGYGWYIKEIATLFLVMGLLAGIAFGKNANDLARSFIAGAKDIMSAALV
                     VGLASGIVVILEQGRIIDSLLNYSAEAMMGYGKVTSIAIMYVFYNLLNLIIASGSAKA
                     ALTIPLMSQFSDLIGISRQTTVTVYQLGGGFTNLITPTSGVMVGVLSIARIPYNKWFR
                     WFLPLMTILIITGFLLLLPTLFVPLSGF"
     gene            complement(8123..8848)
                     /locus_tag="PK049_09390"
     CDS             complement(8123..8848)
                     /locus_tag="PK049_09390"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOK61811.1"
                     /translation="MQNEELANKRNSEIFLEVFTEIERSLKEICKDEYTTNFAELLRK
                     ARGLNQVVNYYASDLKEFSQLRNAIVHTRRQNFIIAEPHPDVVQEVIHIKNLLQNPPV
                     VKTVMKLNPYRVSPSASVKEVLTTFAEKGFMRCPVVSNGKIVGLITAKTIARWLVNSS
                     GIIDNTTVSQLLNYIDNDDYCIVSENTDIVSLIALFNNSVRIGAYLQAALVTLNGKPD
                     SLLVGIITPSDFPSIIGQLKVKH"
     gene            complement(8830..9750)
                     /gene="miaA"
                     /locus_tag="PK049_09395"
     CDS             complement(8830..9750)
                     /gene="miaA"
                     /locus_tag="PK049_09395"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004293810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOK61812.1"
                     /translation="MSVPNKYLIALLGPTGVGKTELSLKLAKHFNSPIVSCDSRQFYR
                     EMRIGTAFPTDWELAQAKHYLVGHKSVTERYSCGMFELDALDSLKQIYLSSDFAILVG
                     GSGLYGDALLKGIDDFPTPDPELRKLLQQQLKTDGIESLRHQLKILDPEYYKQVDLKN
                     PQRVLKAVEVCLQTGRTFSSFLTAPKKERPFKPIKIGLTRPREKLYERINKRVDQMIE
                     QGLVDEVKQLVGFRNYNALNTVGYKEIFQYLDGEITLDQAIELIKRNTRRYAKRQLTW
                     WARDNEICWFNPENQTEIIEYIERLCRTKN"
     gene            complement(9787..10320)
                     /locus_tag="PK049_09400"
     CDS             complement(9787..10320)
                     /locus_tag="PK049_09400"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61813.1"
                     /translation="MIYKFRMLSDEDKLFLRDFEVDADSTFYAFHKAIQENLGFDPGQ
                     LASFFLADEQWNKGMELTLIDMQNDTGMAAIPMERVKIKELMSNRRERLLYVYDIFAD
                     RNLFIELIDIQEPNPDVKYPVCTASVGDAPIQLAEDFAFEENISMEEPNEIDELFDEL
                     NDDDELGSLGLDDKDEW"
     gene            complement(10391..11431)
                     /locus_tag="PK049_09405"
     CDS             complement(10391..11431)
                     /locus_tag="PK049_09405"
                     /inference="COORDINATES: protein motif:HMM:NF017686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HOK61814.1"
                     /translation="MKLEKRINAFSKLGQQLPNAVRDPSHPLGHAAANAQHVNPWFTP
                     ENVLNAVDAIAYEWLQPIELESWIKAYPEKYFTPPTAKTVGVVMAGNIPLVGFHDFLC
                     VLMAGHKINIKLSSKDGDLMGSVAKMLVAIEPAFADCIQITEGPIRDFDAVIATGSDS
                     SVRYFDFYFRNYPSLIRGHRNGVALLSGNETDDELALLADDIFTYFGLGCRNVSKLIV
                     PEGYNFNRLIGSMSRWKHLINHHRYANNYEYNRTILLMNQSPHLDAGFVLVVPNENID
                     APVAMVNYQEYSGINSAIDYINVNDSKIQCVVTKTNIDHSRRVIFGDSQKPKLNDYSD
                     GIDTIEFLGTLG"
     gene            11553..12803
                     /locus_tag="PK049_09410"
     CDS             11553..12803
                     /locus_tag="PK049_09410"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOK61815.1"
                     /translation="MKRILTLYGEIFRISINSILATKLRSILTILIISLGIMALVGIL
                     TAIESIEGSLTNSFTSMGANTFTIQSRGTNVNIAGNRYRKKNYSYISLQQARQFKEDY
                     RFPANVSISVYASGASTIKYMSKKTNPNITVRGVDEEFMKVPGVEIERGRNFSSIDIL
                     DSRHVAIVGQEVANKLFMKNEDPIDKFINVGSGKYRVIGVLKNKGSGFGGGPNRTVYL
                     PYTNVAAFFSRPNMNYSISIQPYDPKLLEAAVSEAEGTFRIVRNLSATDESDFNIEKS
                     DNLAKLLIENLKFVSLAATVIGIITLIGAAVGLMNIMLVAVAERTREIGTRKAIGAKS
                     SEIKQQFLFEAILICQLGGLFGVFLGILIGNLVSVLTNGPFIVPWGWILGGLVISFVV
                     GLASGYFPAVKASKLDPIEALRYE"
BASE COUNT         3860 a         2911 c         2640 g         3934 t
ORIGIN      
        1 ttttagtatt tatgttttta aaatttggct gccaaattaa tcaaatattt atcaccctat
       61 catatatttt ttaacatttt tttacccatt ccttaatttt tgtttcaaaa tgtaaccaaa
      121 actacctgaa atggaaatta ttcctaacta tctaaacaaa tggttgctga tttaaacaaa
      181 tatttgactt gcactccttt tttcgtaaat tgcttaaaat attcatttgg tatgaaaaga
      241 ttgcttattc tattgacaat atttgcccta atcctgaatt tggggtttgc cattcaaaaa
      301 gagcatgtta aaaatgatag cttatcacac aagagtcaaa ctgaggctgt tcatgcaacc
      361 aatatggctt cggaaattga gaaccaaggt gaagtaaccg aaggctcaca tgaagggcac
      421 ggcctaaacc ttgctccttt ccttttcatt attctatcgt tgtttattgg aactgctact
      481 cggcacctgt taagcaaagg gttattacca tacaccatta tgcttttaat tattggactg
      541 attttagggt tgcttacccg atttgatatt cttcaggctg ttggattggg tattattggg
      601 caagcggttc aatgggcagg aaatatcgac ccgcacctta ttctttacct cttcctgcca
      661 acccttattt tcgaggcagc ctatgccctt cacatacaca cctttaaaaa atcgttggtt
      721 aatgcggtaa tacttgccgt tcccggtata atggttgcaa ttgtgataac agcctttgct
      781 cttctgctca tcaagtcgat gggcattggc ttaaacgagt ggacaatact acttgccttc
      841 ctatttggcg ccataataag cgcaaccgac ccggtggctg ttgtggctat ccttaaggag
      901 gtaggagcaa gcaaaaggct ttcaaccatt accgaaagcg agtcgatgct taacgatggt
      961 accgctatcg ttttctttat gaccatattt ggcctaatta cgggcgaagc tgggggtaat
     1021 gcttttgttg agttcttgaa ggtttcgttg ggcggtgtgc ttgtgggcgc agttatcggt
     1081 tggggaatac tttcgtggct taaaaaagta tttaacgatg cgttaataga gataactgtg
     1141 gtgattgggg ctgcttacct gtcgttttac attgccgaat cggttttgca cgtatcgggt
     1201 gttattgctg tggtaacatt gggtatttcc atggcagggc caggaaaaac aaaagtgagc
     1261 cctgaggtta cccacttcct tcatgagttc tgggagttgg cagcatttat tgcaaacacc
     1321 ctaattttca tcatagttgg tgttgttatt gcgcagcagg tcaactacac tttaagcgac
     1381 cttaaagttt tgttactggt atatgttacc attcatattg cacgccttgg aaccatctac
     1441 gttttttacc ccctgatgcg ccgaattgga tatggtatag cctttaaaga ttcaattgtt
     1501 ctatggtggg gtggcctaag gggcgctgta gcgcttgcct tagcactgat tgttgccatt
     1561 gatagccgaa tcccctttga accccgcaac cagttactat cgcttactgc cggtattgtt
     1621 attttgactt cacttataaa cgcaactact gtacgttggc tcattgataa gcttggactc
     1681 tccaaaattg gcgatatcaa agcaggcctt atgatgcaat cgctacaaca aattaggcta
     1741 agcggcgaga aggaaattga aaagctcaaa gaaaacaggt acatgtccgg agccgattgg
     1801 gacaaagtat cgagtttcct tattgatgcc aatgcggtgg aggaaaaacc tcaacacttt
     1861 aacctggaag atgccattgc tgaaacccgt aagcgtctgc tccagaaaga gaaggaaagt
     1921 tactggcgac agttcagcat gggtatgctt agctccgaag gggtaaacct actctctgac
     1981 caaattgatg ccctgctcga ttttggcgga aaaattcccc tttcggaacg acaggatatc
     2041 gaaaatattt ggactacacc caaaacaata gctaagcttc agaatctccc gctagttggc
     2101 agaatttgga aacgtaaatt cctaaaccgt ttagcattga gttatgattg tgccagagcc
     2161 tttgtggccg ctcaggaaga gaaccaaaaa tctctttcga gccttattat tggcttctca
     2221 ttagggggag cggagtcgtc aaaagaaact gaactactta gcgcccttga ggatgagcta
     2281 aacgagaacc gcattactgg gcaaaccttc ctccgaaatt tacgggagaa atacccagat
     2341 atttgtagga gtattgaaac cttacttgcc tcgcgctcac ttctgaacca acaggaagaa
     2401 atgttagaac gtttaaagaa acagggacgc ctggaacccg atgaggtgga acgaattcag
     2461 gcatacatac actcctcaat gaagcaacta attgacacct ccctaaagga gaacacagaa
     2521 ctaaaggtgg ctgctgctct taggaatact cccggcttaa acaatctatc aaaagatgcc
     2581 cagcagttcc taatcactaa agggcagcta aaaatttacc ccgaaggaag tgtcattgct
     2641 aaacaggact ctgagcccga tggtatttat ataatcatca atggtacggt aaattactat
     2701 aaatccgata ccctaaccgg cgttaaggaa atttccgatg tttttggggc atacgagtgg
     2761 tttatatctt ctaaactacg ttataccttt actgctgaaa cccctgttac actgatcaaa
     2821 ataaacactt caaccattaa gttgcttacc agcaagttcc ctgaaacaaa ccgtttgctg
     2881 caattttgcg cttctgccga tatcgctctt gaacttctta aacaaaacga aaaatttagt
     2941 gaacagtcca tccgtaagct cagaaacctt atccgaaatg cagagctatt tgaactgaat
     3001 gatggtaaaa aacaccgttt ttcaggttca gcatgtattc tggttaaggg tcaggctatt
     3061 actgaagagc aggatacctt gttcactgca ccgacaatac ttgaagagtg taactttaga
     3121 agtaatggcg atgctttagc ccttatttta ccttaaggct ttttagcctc aaccatgggc
     3181 tgaaaacgct tgtatgtagg caaagcagcg gggccatacc aaaagaaata ctccgcatcg
     3241 aacaatccga attttactgc aggattggca ttcatcaatt tttgagcctc ttctgttttg
     3301 cttgtgttaa gaatgtaaaa actcctgaag tttttatcat tcttacccat tggccctgta
     3361 actatgagtg ttccgctgtt tgtcatctca tttattgcat caatatggcc cttgtatagc
     3421 cactggagcg agtcgcgact ctttatctgt tttgtaccag tcttaagcat aaccatcaca
     3481 taccacttca taccatactc atcggctcca agggagtcgg caagggcttt gttgtaattg
     3541 gaaacagggg tttgaccctg tgcctgagta gtggttgcaa aaagaattag caacgccgaa
     3601 aagcaaaaca acctcatttt ctgcaacttt attcttatct gtataaaata tcaagccaag
     3661 ttaattaaaa aatgttaatt atgcaacctg aaagctcgta tttatttatc aacagttttt
     3721 taaaaaaatt gctatactta caaccggttt taaaatcaat ttacaatggc tcaaatttta
     3781 cttgttttat tctacttatt aattccgttt gttattctac atctatgtca taagtttcct
     3841 tttgtcaaca aattaggggc tgtatttatt gcatacctgg tggggttaat agtaggtaac
     3901 attggtattt tacccgaatg ggctggaggt attcaagata ccctaacaac cataacagtt
     3961 ccactggcca ttccattgtt gctcttctcc tcaaacctga agcaatggaa aagcatggca
     4021 ggtaaagcca tggtatcgct attaatcgga ataacatcgg tagtagcaat tgtaattgca
     4081 ggttacttta ttttcatggg caaaggaatg gctgatttat ggaaagtaag cggaatgctt
     4141 gttggcgtat atacaggagg tactcccaac ctagcatcgc tcaaaatgat gctaaatgtt
     4201 gatcccgatg tttacatcct tacccatacc tacgacatga taatttccac agttttcctt
     4261 gcgtttttaa tgactgttgg gaaaaagttc ttctcgctat tcctgccagc cttcaaggat
     4321 gataacatgg aggaagttga gtacactaac ggcagcgacc cattctgggg aatttttaag
     4381 cgtgaaatat tttacccctt gctaaaagca tacggattaa ctattcttat tttcgccatt
     4441 gctggtggtt tatcgctttt ggtgcctaaa aatagccaag ttgtggttgt tatacttacc
     4501 attaccacat taagtattgt tgcatcaacc tttaaaaaga taaacgccat tgaaaaaacc
     4561 tttgaatcgg ggatgtacct tatactgata tttagcgtgg ttgtggcctc catggccgat
     4621 ataagccggt ttgccggctt aacacccggg ttatttggat acattaccct tgcagtattt
     4681 ggttcgcttt tccttcaggt tgtactagga aaaatattca aggttgatgc cgataccacc
     4741 ataatcacct ccactgctct aatctgttct cctccatttg ttccggttgt tgctgcagca
     4801 attaacaatc gaaaagtaat tctatcgggg ataagtattg gaattattgg ctatgctgta
     4861 ggaaattacc ttgggtttgc catagccgag cttctaaaaa caattcaata aaaaagccgg
     4921 ccttaggccg gctttaaaat ttcagttagc agctagaata aaaagttaag tccaatgtag
     4981 gttcctgata gtccatcgtc cttggtcata gcgcgaccat ggtcaactac aattacaaag
     5041 ttttggttca tcacaacacg caagccaagt ccccaactgg tatgcaattt ctctttgtca
     5101 ttgctaaagt aatccgatgg ggagtatgga acctcaataa aattgggtac ctcaacatca
     5161 accttacgta ccacctgacc accatctaca aaagcgttaa gcgataggta aaagttttgg
     5221 ttgatgaagt gaaagcgggc gaacttccaa cgcatttcaa agttgccata ggcaattcca
     5281 tcgcccacaa cccggttacg aattatacca cgcaagcttt tagcaccacc aagcccctcg
     5341 ttggttgcgc cccttaagaa aattgttgtc atattgggtt gcaggtagaa cgggcatgtt
     5401 ccgaaaaggg ttccctgata acccagcctg taaacaaacg atagcctctc aggaactaat
     5461 gtgaaatact ggcggtgaat aatacttagc tttgcatgct ggtacttact tccaatgcct
     5521 aaaaacgacg gagcataggt tataaccgcc tcggtccaca tccctttcat gggattgggt
     5581 tcgttatcgc gagtatcgta aaccgcacca actttcaggt aggtaacaaa tccaccattc
     5641 ttttcatctt caccaatcaa tccccagtta atgtagcgct ggtaaagtcc acctcctaca
     5701 tagggtaact tatcggcttc atcttttccc ctattcagct tgtcaacatc aaccgtatca
     5761 attttaaaat catacaggcc tataccggca acccagccaa acttttcgtt ttttagtttc
     5821 ccttgcaagt cagtagtaaa cctcaacagt ttcctatcgt acttatagaa tgcacggctg
     5881 cggtaatcgg cgctttcgtc atcggcccag tccaaattga ataccgattc gtaaccgtta
     5941 aaaccataaa agtcgtacat cttatcagtt aaatacgaaa ggtcagcagt taccctgagc
     6001 ccgggaataa catactttga atcgtaaaac aattggttta taccactacc ctttgtgaag
     6061 cgcgaaacct cagcgtaaac attatgcttg tacttgggat agattgtacc atcgccgtag
     6121 tggaacaggt ttaccaatgc tccatactca aaacccaggt cggtatcgta ggctaccacg
     6181 ggtaaccctc caacattcca acccgatttt actttttcag cttttttgtc atcgctggtt
     6241 tgctgggcaa ataatcccgt aacggtaaac aatattgcag caagtagtat taacttcctc
     6301 atggaattgg ttttatgatt ttctcaaaac taaggatttt tttaaaatag gtagatacta
     6361 ctaatcgaat gaatagaaat atttatgctt tgcagatatt aatgttcttg acacctggtt
     6421 cagaatttaa cagtaattgc tggcctctac cactagaacc cgctaagtgg aacgaataga
     6481 gttggtaaaa gtagcaggaa tccggtaata ataagtattg tcattaaggg caggaaccat
     6541 ctaaaccact tgttgtaagg aatacgcgca atgctaagaa ctccaaccat caccccagag
     6601 gttggggtaa tcaggtttgt gaaaccaccc ccaagctggt aaacggttac cgtagtttga
     6661 cggcttatac caattaaatc ggaaaactgc gacatcaatg ggatagtaag tgcggccttg
     6721 gccgaacctg atgcaattat gaggttcaac aggttgtaaa acacatacat aatggcaatg
     6781 gatgtaactt taccgtaacc catcatagcc tcggccgagt agttcagcag ggaatcgatt
     6841 atacggcctt gctccagaat tactacaatt ccgcttgcca aaccaacaac aagcgctgcc
     6901 gacataatat cctttgcccc agcaataaac gatcgcgcaa ggtcgttggc atttttacca
     6961 aatgctatcc cagccagcaa acccatcacc aggaagagtg ttgcaatctc cttaatatac
     7021 cagccatatc ccatcacacc aacaattaaa aacagaatgg taaacagtaa gatgtttact
     7081 atgaaaagct gaaccgaatg cctcaatgct aaaattccaa ctaaaatcca tactactgta
     7141 gcaatcggaa taattgggac agagatgata ctttgcccca caaccaaatg gcttacaggg
     7201 tagtagtaag caaatatggc aaacactaaa gccaaaccaa taaatgtagc ccacgcactc
     7261 ttaccaggtt ttgatttggt tccgttttca tcctgggctg ccttgtgcct ccagtactca
     7321 tcaatttcat atacgggcga tagctttgga ttctttttaa tccttcgggc ataccaaatc
     7381 acatagccaa tgcctatagt atttataatc acccatataa caaacctata ctcaatacct
     7441 gagaacaact gtattcccga caaaccctgc gcaataccaa tggtgaaagg gtttagcaag
     7501 gcgcttgcaa agcctaaacc cgcacccagg aaacataaac ttattcccac aatggaatcg
     7561 tagcccatac ttatggcaag cggaacaaaa atcaccacaa aggcaatggt ttcctcgctc
     7621 ataccaatta ccgccccaaa aaagctgaag caaatcataa ttagagctat aaccagatta
     7681 tcaactccaa tcacctttaa gggtttaaac ctctcaatac gtttggtaaa attcaggaac
     7741 gaaagaatag caacatcaag tgccttactc tcgttgagca accaaaatgc cccaccaatc
     7801 atcaggatat aaaaaatgat atcgtaggtt cgattcattc cttgaaaaat agatgtaaac
     7861 acctgccagg tttgaggttg attatcaacc tgatggaagg aacccgggac aatgacctca
     7921 cggtcgatac cgttaacgtt ctttacctcc ctggcaaatt tacctcctgg aacaaaccag
     7981 gtcaaaacgg cagcaaaaac aataagagca aaaataatta cataggtatg cggaaattcg
     8041 cgctttttct tttcctgttc catgttcata gtgttttgat gtaatacggt ttcaaaacta
     8101 attgccccta atctgcacaa aactaatgtt taacttttaa ttgcccaata atagaaggga
     8161 aatcgcttgg ggttattata ccaacgagta aactgtcggg tttaccgttc agcgttacta
     8221 aagccgcttg caggtaagca cctattcgga ctgaattatt gaaaagggct attaacgata
     8281 ctatatcggt gttttccgat actatgcagt aatcgtcatt gtcgatataa tttaaaagtt
     8341 gactaaccgt tgtgttatca attattccag agctattaac caaccatcgc gcaatggttt
     8401 tggcagttat aagtccaact attttaccgt tactcacaac cgggcatcgc atgaaaccct
     8461 tttcggcgaa ggtggttaaa acctccttaa ccgaagcact gggtgatact ctatagggat
     8521 ttaacttcat tactgttttc acaaccggcg ggttttgcag caggtttttg atgtgaataa
     8581 cttcctgcac aacatcggga tgtggttcgg ctataatgaa gttttgccta cgcgtatgaa
     8641 caatggcatt gcggagttgt gaaaattctt ttaaatcgga agcatagtaa ttcacaacct
     8701 gattcaaacc acgcgccttg cgcagtagtt cagcaaaatt ggttgtatat tcgtccttac
     8761 aaatttcctt taggcttcgt tcaatctccg taaaaacttc aaggaatatt tcggaattgc
     8821 gcttatttgc tagttcttcg ttctgcataa tctttcgatg tattcaatta tttcggtttg
     8881 attttcgggg ttaaaccaac aaatctcgtt atccctagcc caccatgtca gctgccgttt
     8941 ggcataccga cgggtattcc gtttaataag ctctatagcc tgatcaaggg ttatttcacc
     9001 gtcgaggtat tgaaatatct ccttgtaacc aaccgtatta agggcattat aatttctaaa
     9061 acctacaagt tgctttactt catccactaa accctgctca atcatctggt caaccctttt
     9121 atttattcgc tcatacaact tttcgcgggg tcgggttaaa ccaattttta tgggtttgaa
     9181 tggtcgctcc tttttgggtg cggttaagaa tgagctaaag gttctgcctg tttgcaggca
     9241 aacctcaact gcctttaata ccctttgcgg attcttcaaa tcaacctgtt tgtagtattc
     9301 agggtcaagt attttaagct gatgccttaa gctctcaatg ccatcggttt ttagctgttg
     9361 ctgtaacaat tttcgtagct cagggtcagg tgttggaaag tcgtcgatgc cttttagcag
     9421 agcatcacca taaagcccgg agccgcctac aagtattgca aaatcgctgc taaggtagat
     9481 ttgtttaagc gagtcaaggg catcgagctc aaacatgcca cagctgtaac gttcagtaac
     9541 cgacttgtgc ccaacaaggt agtgttttgc ttgagcgagt tcccaatcgg tagggaaagc
     9601 agtccctatt cgcatttccc tgtaaaactg tcgggaatcg cacgaaacga ttggtgaatt
     9661 aaagtgtttg gccagcttaa ggctcaattc cgtttttccc acaccggttg gtcccaaaag
     9721 ggcaatcagg tatttattgg gcacggacat tcatcaaatc cagttaaatg gatatagttt
     9781 gataaactac cactcatcct tatcgtcaag acccaagcta ccaagctcat cgtcatcgtt
     9841 gagttcatcg aaaagctcat caatctcatt gggttcttcc atggagatat tctcctcaaa
     9901 agcaaaatcc tcagcaagct gaataggcgc atcgccaacc gatgcggtgc aaacaggata
     9961 ctttacatca ggattgggtt cttgtatatc aatcagctct atgaaaaggt ttcggtcagc
    10021 aaaaatatcg taaacgtaga gcaagcgctc cctacgatta ctcattagct ctttaatctt
    10081 gaccctttcc atgggtatag cagccatccc ggtatcgttc tgcatgtcaa ttagtgtaag
    10141 ctccatcccc ttgttccatt gctcatcggc caaaaagaac gaggctagct gaccagggtc
    10201 aaaacctagg ttttcctgaa ttgccttatg gaaagcataa aaagtactat ctgcatctac
    10261 ctcaaaatcg cgcaggaata gcttatcctc atccgataac attctaaact tgtaaatcat
    10321 atcaggtagt ttatcaatac aaaaatagca tcaatattaa acatttctaa aaatttcaag
    10381 ggaaaaacct ttaccctaat gttcctaaaa actcaatggt atctattcca tcggagtagt
    10441 cgttcagctt aggtttttgg gagtcgccaa atataaccct ccggctatgg tcaatattgg
    10501 tttttgtgac cacacattga atttttgaat cgtttacatt gatataatca attgctgaat
    10561 ttattcccga atactcctga tagttaacca tggcaaccgg agcatcgata ttctcatttg
    10621 gcacaactag cacaaaaccc gcatcgaggt gtggactttg gttcattaac agtattgttc
    10681 tgttatactc gtagttattg gcatacctat gatgatttat aagatgtttc cacctactca
    10741 tactaccaat taacctatta aaattatatc cttcagggac aattagtttc gaaacatttc
    10801 tacatcccaa cccaaagtag gtaaaaatat catcagcaag caatgctagt tcatcatctg
    10861 tctcattgcc cgacagcaat gccactccat tcctatgccc gcgtattaac gaagggtagt
    10921 tccggaagta aaagtcaaag taacgcactg agctatcgct acctgtagct ataaccgcat
    10981 cgaaatccct tattgggcct tcggttattt ggatacaatc ggcaaatgct ggttcaatgg
    11041 caaccagcat tttagcaact gaacccatca aatccccatc ctttgacgaa agcttgatgt
    11101 ttattttatg gcctgccatg agaacacaaa ggaaatcgtg aaaccccaca aggggaatat
    11161 tcccggccat aaccacccca accgtttttg ccgttggcgg ggtaaaatat ttttcggggt
    11221 aagctttaat ccaactctct agttcaatgg gttgaagcca ctcataggca attgcatcaa
    11281 ccgcattaag cacattttca ggggtaaacc aaggatttac atgctgtgca tttgcagcag
    11341 catgacccaa cgggtgactc gggtcacgaa ctgcgttggg caattgttgt ccaagcttac
    11401 tgaacgcatt aatccttttt tctaatttca tgtcgaatgt ttattgatct gcaaacctaa
    11461 tcgatttttt gtttcagttt gtatgattta gcttttagtg aaccagaaag tttaaactta
    11521 tacctaaatt tgaaaaagaa atattcaaaa acatgaagcg gattttaacc ctatacgggg
    11581 aaatttttcg aatttcaata aattcaatcc tagccacaaa gcttaggtcc attctcacta
    11641 ttctaataat ctcattaggt ataatggctt tggtgggcat cctaaccgca attgaatcaa
    11701 ttgaaggaag tcttaccaat tcatttacca gtatgggtgc aaataccttt accattcaga
    11761 gtcgtggaac taatgtaaat attgcgggaa atagatacag gaagaaaaac tattcataca
    11821 tctccttaca gcaagcacgt cagtttaagg aggattaccg attccctgcc aatgtatcca
    11881 tttcggttta tgcatcgggc gcgagcacca ttaagtacat gtccaaaaaa actaatccca
    11941 acattactgt tcggggtgtt gatgaggagt ttatgaaagt accgggtgtt gaaattgaac
    12001 gaggccggaa tttctcatca atcgacatac tggatagccg acacgtagct atcgttggcc
    12061 aagaggtggc taacaaacta tttatgaaga acgaagaccc aattgataag tttatcaatg
    12121 tagggagcgg aaagtacagg gtgattggtg tgctcaaaaa taagggttca gggtttggag
    12181 gtgggccaaa ccgtacggtt tacctgccat acaccaacgt tgccgcattc ttttcgcgcc
    12241 caaacatgaa ctattcaata agcatacaac cctacgatcc taaactgctt gaagctgcag
    12301 taagcgaagc cgaagggact tttcgaattg tacggaatct ctcggcaacc gatgagagcg
    12361 attttaacat cgaaaaaagc gacaatcttg ccaaacttct aattgagaac ctgaagtttg
    12421 tttcattagc agccacagtc attggcatca ttacgcttat aggcgcagcg gttgggctta
    12481 tgaacattat gctggtagcg gtggcagagc gcacccgcga aataggcacc cgtaaagcaa
    12541 tcggtgcaaa atcgtcggag attaaacagc agttcctctt tgaggccatt cttatttgcc
    12601 agcttggtgg tttatttggc gtattccttg gaattctaat aggtaacctt gtttcagtgc
    12661 ttaccaatgg cccgttcata gttccctggg gttggatatt aggcggtttg gttatatcgt
    12721 ttgtggtagg tttggcctcg ggctatttcc ctgctgtaaa ggcctcgaaa ctcgacccca
    12781 ttgaagcgct taggtatgaa tagggcatag ccatttggaa tgccgcagaa accatcatta
    12841 tgaattaaag atgttaaaac tatagtcttc atttgaaaaa tcaaatgata aatcaatgtc
    12901 gtttagttga ggatatttgt catattgagt ggtagcaaaa tacccctctg tcatgctgag
    12961 cgctgccgaa gtatcttttt gtaccttaaa tccgctaatt actttttgat tggttttctg
    13021 gcaaccactc cttgttatgc ttttctgttg caatgcaacg tttcagtatt gctcgttgga
    13081 atgtcatagc gtgcagtgct atacatgttg gagtagccat gaagttgcaa atatttagcc
    13141 ccttcggggc tgaaaacgtt gttcgttgtt cgtgaatcgt tgttcgtgaa tatcacgtca
    13201 cacgctctgt gaaacaccgt attacgctgt gtaactctgt gatacttgct gtggctcgat
    13261 tcgtgaaact gttgtcatac ttagcccagt cgaatcatct cttttctctt ctacacccac
    13321 ttgccgttgc ccgatatcct tcgct
//
LOCUS       DAOSAL010000072        13142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_3198_length_13142_cov_46.667533, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000072 DAOSAL010000000
VERSION     DAOSAL010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 13142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13142
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3198_length_13142_cov_46.667533"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            405..3188
                     /gene="leuS"
                     /locus_tag="PK049_09415"
     CDS             405..3188
                     /gene="leuS"
                     /locus_tag="PK049_09415"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOK61816.1"
                     /translation="MDYNFKEIEQKWQDYWRKNKTYRVDVDPSRPKYYVLDMFPYPSG
                     AGLHVGHPLGYIASDIYSRYKRLNGFNVLHPMGYDAFGLPAEQYAIQTGQHPAITTDN
                     NIKRYREQLDKIGFSYDWDREFRTCDPKYYKWTQWAFIKMFKHWYNNKTQKAEPIENL
                     IAEFEQNGNINVDAACSEVRTFSASEWKAMSEKEQQEILLAYRLAYLADVMVNWCPAL
                     GTVLANDEVKEGVSIRGGHPVEQRKMRQWCLRISAYAERLLNDLDQLDWTDSLKDIQR
                     NWIGKSEGAEVWFRIDGTDRKILIFTTRPDTIYGATFMVLAPESELVDELTTPEYAEK
                     MEQYRQEVKRKTERERMAEARKVTGQFTGSYAINPFTNERIPIWVSEYVLAGYGTGAI
                     MAVPAHDSRDFAFAKTFGLPIIQTVIRPGEQPTNPESWTESYDSKEGVVINSPLINGL
                     EVKDAISKICEVIESRGLGKRKVNYRLRDAIFSRQRYWGEPFPIFYKDGMPYPLPEEK
                     LPLELPEIDAYLPTEKGEPPLGRAKNWHTPEGYPYELSTMPGFAGSSAYYLRYMDPHN
                     DNALVSAEANKYWENVDLYIGGTEHAVGHLIYSRYWNKFLYDLGYVCKQEPFKKLINQ
                     GMIQGRSNFVYRVKGTNQFVSHNLKDKYDVTPIHVDVNIVSNDILDIEAFRKWRPEFE
                     NAEFILEDGKYVCGWAVEKMSKSMWNVVNPDTIVERYGADTLRMYEMFLGPLEQSKPW
                     DTNGIDGVHKFLRKFWRLFHNNANEFEVSANEPTAQELKVLHKTIKKVTEDIEKFSFN
                     TGVSAFMICVNELSDLKCNKRAILEPLTVLIAPYAPHIAEELWHLLGHGTSVNTAAWP
                     KFNEEYIKESTFTCPISFNGKTRFTLELPLNLKADEVEKIVLADENTLKYLDGKQPKK
                     VIVVPNKIVNIVV"
     gene            3289..3708
                     /locus_tag="PK049_09420"
     CDS             3289..3708
                     /locus_tag="PK049_09420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOK61817.1"
                     /translation="MDKSDNKTKSFKDLVVWQKAHAFVLNIYQQVKIFPDDYQAIVGD
                     MLCESATAIATNIVGGYKKKDREDKLLFLTTAQEALEECRYYITLATDLHLLDSFQAD
                     ELENNLNEVSYLLNSYARSIKRRKEGDYKKDDDLRDE"
     gene            3726..4604
                     /locus_tag="PK049_09425"
     CDS             3726..4604
                     /locus_tag="PK049_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOK61818.1"
                     /translation="MTTANIFKTVRSYAIIVFGLLLYALSWTAFLIPHKITGGGVSGI
                     GALVYYATGIPMGYTYFLINVGLILLAIKMLGANFGVKTIFGVTVGAFLLSLLQMTIK
                     VAVVEDKFMSTIIGGALAGVGLGVVFTQGGSTGGTDIIAMIINKYRNISPGRIIMLCD
                     VFIIGSSFLVLLDLEPAKRIETIVYGYVAMAITAYSLDAVLSGSKQSVQVFVFSKRYQ
                     EIADRITSEINRGVTVVDGMGWYTKESQKVLISLVRKHEVSDVYKIIKEVDPEAFISV
                     ATVTGVYGRGFERIRH"
     gene            complement(4601..6139)
                     /locus_tag="PK049_09430"
     CDS             complement(4601..6139)
                     /locus_tag="PK049_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007482875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOK61819.1"
                     /translation="MLVKTFGSAVHGIKATTITIEVSVGQGVNFFLVGLPDSAVKESQ
                     QRVMSALETNGYKFPKKRIVINMAPADIRKEGSAYDLPLAIGIMAASEQIKPDLLPDY
                     VIMGELSLDGTIQPIKGVLPIAIQAREEGFKGFIVPAQNAREAAVVNNLDVYGVESIK
                     QVVDFFNGNSTLEPTIVNTREEFFAHANNYDVDFSDVKGQENVKRALEVAAAGGHNII
                     MIGPPGAGKTMLAKRIPTIIPPLTLREALETTKIHSVAGKIDRNTSLMTRRPFRSPHH
                     TISDIALVGGGQFPQPGEISLAHNGVLFLDELPEFKRTVLEVMRQPLEDRVITISRAK
                     FTVEYPASFMLVASMNPCPCGYYNHPEKECVCSPGVVQKYLNRISGPLLDRIDIHVEV
                     IPVPFDKLASAPPAESSAQIRERVVKARQIQEERFVPWADVHCNAQMTSKLIRQFCVL
                     DEAGTAILKTAMERLNLSARAYDRILKVSRTIADLEQSEKILPQHVAEAIQYRSLDRE
                     NWAG"
     gene            complement(6186..6554)
                     /locus_tag="PK049_09435"
     CDS             complement(6186..6554)
                     /locus_tag="PK049_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61820.1"
                     /translation="MRKKLLVAPIILGAVLAFLTWGCEKEEEDVCQIFEPPQCEIANV
                     CCPTDGGNCYYEVGDSKYYCDKTKATPDDPDGCNDAENQAIEVLCEAASPDQLMNAKV
                     ELSKLTRKLMQEARTLSVCH"
     gene            complement(6559..7212)
                     /locus_tag="PK049_09440"
     CDS             complement(6559..7212)
                     /locus_tag="PK049_09440"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOK61821.1"
                     /translation="MKKRMGRKLRPIEWIAVAISIVLIVLIGIKAWQLAPNKTSKLAA
                     PISSNPLDITFGSDSAQLAIYAYISYHCGYCRLFFTEVYPELEREFISTGKVRLVLKI
                     VEHSNNADRINEVKTAVCINRYGYYGKLHELLVTNSSVIYSDEFREMVNHFIDSDPLV
                     AECILGNESMDYILQIRNEFDKFGFTGTPTFVIEGRVFRGYMPFEQFKSIIVKHLNQ"
     gene            complement(7175..10246)
                     /locus_tag="PK049_09445"
     CDS             complement(7175..10246)
                     /locus_tag="PK049_09445"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HOK61822.1"
                     /translation="MKTKRFIVRLLKYFGLTIIFLHLLVFIIIGIAKLFEGSIIRLAV
                     KEIESNLKAPISYGEVSLVPFRDFPNLTVRIADFKLGQQADSVRVFGIGSLPDTLVGF
                     KNVFVSVKAYPLLKDKVIINGVELNGFRVNYLVDSTGKSNFDFLIPPDTVETPVDTVT
                     QTILNILLKNLTLSDIHLTYSDQKLKAAADIVIPKLHLEGRVSGDSIWGKSNGEIVVT
                     NTRFADLPLEKVTNLNFSYSVDYDNGNLLLDYAKFDINGIELKATGNATLSDSIWVDF
                     SLALPKLNLAELYGFIPESMIKEYGVSRLGGTLKFNTIVRGYLYDTLMLPSVFVDFDL
                     RKGVVETKQYPKVNKLDFEGSVSIPNPNYLATSAVNIKKFNLETQQSRANLTLTLKNP
                     NKPLYNIQGDMHLVLDEFAPLLPDSTLTELSGLIDAKFFTKGKMPEYLDVNSSDYFMD
                     NTGFSLSFSNINAGVDTVNAVKELNAEVTYSPGKELTVNNLSLRLPAYKVSLKDGLLK
                     VKVIGRISELDKIDVRVDTLHIAMGNSSFSSKMKFKNLKSPVFRGEGRLYVDLDELHR
                     FIPDTLVSLVTGKIDFGFSTSGTINPDSIDKQIMPILFENSMFAAQIKDFGFSLPNDS
                     LIGVDRLSLTLSMANDTIRLANVNGHYKNLDFRSDSTSIWNVYKTFILGQKDKKLIVN
                     TSLWANYVDYDIFLPFMAEDSTASPDTAASTIPQYIIRGIAKADKVKYGKVLIDDLST
                     KFRVDDSLYVLDELRMKAFGGNMVTSLVYDTRKYPLEIIEFKNESNRLNIKQLLEQND
                     DFEQDYITHKNIDGLVTGTVFGRIVMQDTNLLYDKMNLLGNFKLENGGIYNFEPAMEL
                     SKFTNLNELNNIVFNTLETSVFIYNNQIYFPKTNIVSTAMDMSVYGMQSFADDYEYHF
                     VVYPGDVMLGKSKKLLKKQGLKDEGFEGPNKAKRSGLYLVAFERGKESKYGFDTKELQ
                     ATMKATIRVQERGLNLVFHPHVMNFSTELDRKESKRKTADEKKDGKEVKTN"
     gene            complement(10265..10810)
                     /locus_tag="PK049_09450"
     CDS             complement(10265..10810)
                     /locus_tag="PK049_09450"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOK61823.1"
                     /translation="MRISVKYLMVVSIMVLCLSGYSQEISVGQVVPDITGYGPNGEEF
                     KLTSLRGKLVLVDFWASWCSPCRKENPVLAEVYNKYKDACFTNGNGFAIFSVSLDLKR
                     DNWVNAIADDKLVWPYHISDLKGWRSEAAKAYSVNAIPMNFLVDSSGVVIAKNLRGSE
                     LSNTLRKYRKWKFFSNSDCKN"
     gene            complement(11356..12351)
                     /locus_tag="PK049_09455"
     CDS             complement(11356..12351)
                     /locus_tag="PK049_09455"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HOK61824.1"
                     /translation="MRRNKHIGIVAVMALMGVAGAVKGQQQPLYSQYMLDPFLVNPAV
                     AGAEGVTAVNLTARQQWIGYSEAPSTYALSAQTRILKTSFRNRSRLIKTRVRRRRPSG
                     RVGLGAFVFNDNNGRIHRTGFQATYAYHIYMRDQQLSFGASLTSFQFKANVNAIDGYE
                     QTNDPLLMGKTSKFAPDANLGVLLSTQKYYVGFSATSLFQSFIQFGGGNSEVAYRLLR
                     QYYLVGGYRYQPYRSDFAIEPSVLLTTNERFQFGADLNLKGYYKNDYWAGVSIRSTGA
                     IITMIGVKYQQFYFGYAFDYNFTDVSTFSKAGSHELMLGMKLGDTARRYRWLNRF"
     gene            complement(12507..>13142)
                     /locus_tag="PK049_09460"
     CDS             complement(12507..>13142)
                     /locus_tag="PK049_09460"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOK61825.1"
                     /translation="DGNTNQVYYTGESGTYTVRLVNSNYSCSYDITEVTVRPLPVVDL
                     GNDTTLFANQTLLLDVYNPDFTVYDWSTGAITPSITVDGQSGDQTIWVKVTDNYGCSN
                     SDTIQVSAADYSNLRIPAAFTPNKDGVNDTWEFPAPYKGQDLRGYITNVDVKVFNRWG
                     KMVWSSKGAYKPWDGKDLNGKELPMDSYHYIIVFTVGEKKYEYKGSVTIIR"
BASE COUNT         3601 a         3010 c         2792 g         3739 t
ORIGIN      
        1 ttatttttgt ccatacacat aagtttgtgt agacattttt caggacgggt catttattaa
       61 cgtttaacgc ctcacggctc acggttcacg ctaaattatt cgcattcctt taaattcatc
      121 taacttcatc taacttcctt taacttcatc taacttcatc taacttcctt taacttcatc
      181 taacttcttt taacttcctc taactccttc tccttctgtt ttttattctc gtttaacgtt
      241 taacgattca cggttaacgc cttacggttc acgtctcacg gttcacgctt aacgcacact
      301 catccttatt caattctaac tattccccat ttccccctct tactttaacc tcttttttct
      361 atctttgcag gctgaaatca actttaacaa aatcaggctt ttatatggac tacaatttta
      421 aagagataga acagaaatgg caggattact ggcgtaaaaa caagacatat agggttgatg
      481 ttgacccctc aaggcccaag tactacgtac ttgatatgtt cccatacccc tccggagccg
      541 gattacatgt aggtcatcca ctgggataca ttgcatccga tatttactcc cgctacaaaa
      601 ggcttaacgg ctttaatgtg cttcacccca tgggctacga tgcctttggc ctaccggcag
      661 agcagtatgc cattcaaacc ggtcaacatc cggccattac caccgataac aatattaagc
      721 gctaccgcga gcagctcgat aagattggct ttagctacga ctgggatcgc gaattccgaa
      781 cctgcgaccc caagtactac aagtggaccc agtgggcttt tattaagatg ttcaagcact
      841 ggtacaacaa caaaacccaa aaggctgagc ccattgagaa ccttattgct gagtttgagc
      901 aaaacggtaa tattaatgtt gatgcggcct gtagcgaggt tcgtaccttt agcgcctcgg
      961 aatggaaagc catgagcgaa aaggaacagc aggagatttt gcttgcttac cgcctagcct
     1021 atcttgccga tgttatggta aactggtgcc ctgcactggg aacagtactg gcaaacgacg
     1081 aggttaagga gggtgtctcc attcgtggcg gccatccggt tgagcaacgc aaaatgcgtc
     1141 agtggtgtct caggatatcg gcttacgccg agcgactact caacgatctt gaccagctcg
     1201 actggactga ctcgcttaag gatattcaac gcaactggat tggcaaatcc gagggtgccg
     1261 aggtttggtt ccgaattgat ggcactgacc gtaaaatact aattttcacc acccgtcccg
     1321 ataccattta cggcgcaacc tttatggtgt tggcacccga aagcgaactg gttgatgagc
     1381 ttactacccc cgagtatgct gagaaaatgg agcaataccg tcaggaggtt aagcgaaaaa
     1441 ccgagcgtga gcgcatggcc gaagcccgca aggtaaccgg tcagtttacc ggtagctacg
     1501 ccattaaccc attcaccaac gagcgtatac ctatttgggt aagcgaatac gttttagctg
     1561 gctatggaac cggcgccatt atggcggttc ctgcccacga tagccgcgat tttgcctttg
     1621 ccaaaacctt tgggctacct attatccaaa ccgttattcg cccaggcgag caacccacta
     1681 accctgaatc gtggacagaa tcgtacgatt caaaggaggg tgttgttatc aatagcccac
     1741 ttatcaatgg cttagaggta aaagatgcca tttcaaagat atgtgaggtg attgaatcgc
     1801 gcgggctggg caagcgtaag gtaaactacc gcctacgcga tgccattttc agccgccagc
     1861 gatactgggg tgagccattc cctattttct acaaggacgg aatgccttac ccgctgcccg
     1921 aggaaaaatt accccttgaa ctccccgaga ttgatgccta cctacccact gaaaagggcg
     1981 aaccacccct tggtcgcgcc aagaactggc atacccctga gggatacccc tatgagttaa
     2041 gcactatgcc cggctttgcc ggctcctcgg catactacct ccgttacatg gacccccata
     2101 acgataatgc ccttgtttcg gccgaagcta ataagtactg ggaaaacgtt gacctttaca
     2161 ttggcggaac cgagcatgcc gtgggacatc ttatttactc aaggtactgg aataaattcc
     2221 tatacgacct tggttatgtt tgcaagcagg agccctttaa aaagctgata aaccaaggaa
     2281 tgattcaggg tcgatcaaac tttgtttacc gtgttaaggg aacaaaccaa tttgtatcgc
     2341 acaacctgaa ggataagtac gatgtaaccc caatacacgt tgatgttaac attgtaagca
     2401 acgatatact tgatattgag gcattccgta agtggcgtcc ggagttcgag aatgctgagt
     2461 tcattctgga agatgggaaa tacgtatgcg gatgggcagt ggaaaagatg agcaagagta
     2521 tgtggaatgt ggtaaacccc gataccattg ttgagcgcta tggtgccgat accctccgca
     2581 tgtacgagat gttccttggc cccctagagc aaagtaaacc ttgggataca aatggtattg
     2641 atggtgtaca taagtttctc cgaaagttct ggaggttatt ccataacaat gccaatgagt
     2701 ttgaggtatc agccaacgaa cctactgcac aagagctcaa ggttcttcat aaaaccatta
     2761 aaaaggttac tgaggatatt gagaagttta gcttcaatac cggagtatcg gcctttatga
     2821 tttgcgtaaa cgagctctcc gatttgaaat gtaacaagcg cgccatactt gaaccgctaa
     2881 ccgttttaat tgctccctat gctccacata ttgccgagga gctatggcat ctgcttggac
     2941 atggaacatc ggttaatact gccgcttggc ctaagttcaa cgaggagtac attaaggaat
     3001 cgacctttac ctgtcctata tcgtttaatg gtaaaacgcg ctttaccctt gaactaccgt
     3061 taaaccttaa ggccgatgag gtagaaaaaa ttgttttagc cgatgaaaac acgctaaaat
     3121 acctcgatgg caagcaaccc aaaaaggtaa ttgtggttcc aaacaaaatt gtaaatattg
     3181 ttgtttagtc aaataatatt tttgcagatt tcaaaaaagt agtaacttta gtaaaccctt
     3241 attaatcatt attatactta caaaacattc aacagaaagg agtgcgatat ggacaaatcg
     3301 gacaacaaaa ccaaatcgtt caaggacttg gtagtatggc aaaaggccca cgcttttgtg
     3361 cttaacattt accagcaggt aaaaattttc cccgacgatt atcaagccat tgtgggcgac
     3421 atgctttgtg aaagtgccac tgccattgca accaacattg ttggaggcta caagaagaag
     3481 gatcgcgagg acaagctact ctttttaacc accgcacagg aggcccttga ggagtgccgt
     3541 tactacatta cccttgccac cgatttacac ctcttggatt cattccaagc cgatgagcta
     3601 gagaataacc tcaacgaggt tagctacctg cttaactctt acgcacgttc catcaaacgt
     3661 cgtaaggaag gtgattacaa aaaagatgat gatttaagag acgagtagta ggaatttttt
     3721 agagaatgac tacagccaat atttttaaaa cagtccgatc ctatgccatc atagtgttcg
     3781 ggctgttgct ttatgcgcta tcgtggactg cttttttgat tccacataaa attactggcg
     3841 gaggagtttc gggtataggt gcgcttgttt actacgctac tggcattcca atgggttata
     3901 cctacttttt aataaatgtg ggactcatcc ttttagccat taaaatgctg ggagccaact
     3961 ttggggtgaa aaccattttt ggcgttactg ttggcgcatt tctactatcg ctgctccaaa
     4021 tgacaataaa ggttgcagtg gttgaggaca agtttatgtc cacaatcatt ggcggtgcgc
     4081 ttgcaggtgt tgggcttggg gttgtgttca ctcagggtgg cagcactggt ggtaccgata
     4141 tcattgccat gataatcaat aagtaccgta acattagccc aggccgtata ataatgcttt
     4201 gcgatgtttt catcattggc tcatcgtttt tggttctact cgaccttgag cccgccaagc
     4261 gtatcgaaac cattgtttat ggttatgtgg ccatggccat cacggcttac tccctcgatg
     4321 ccgttctttc gggtagcaaa cagtcggttc aggtgtttgt cttttcaaag cgataccagg
     4381 aaattgccga taggattaca tctgagatta accgtggagt aaccgtggtt gatggaatgg
     4441 gctggtacac aaaagaatcg caaaaggtgc taatctcact tgtgcgcaag cacgaggtta
     4501 gcgatgtgta taaaataatt aaggaggttg accccgaagc gtttatttcg gttgcaacgg
     4561 ttaccggggt ttacggaagg gggtttgaac gtatcaggca ctagcctgcc cagttttccc
     4621 tatccaagct acgatactga attgcctcag ccacatgctg gggcaatatt ttttcggatt
     4681 gctcaaggtc ggcaatggta cgggaaacct ttagaattct gtcgtaagcc cttgctgaaa
     4741 ggtttagccg ttccatggct gttttcagga tggccgttcc ggcctcatca agtacgcaga
     4801 actgccgtat cagcttggag gtcatttgag cgttacagtg tacatcggcc cagggaacaa
     4861 aacgctcctc ctgaatctgt cgggctttta ccacccgctc cctaatttga gcgctactct
     4921 cggctggtgg tgccgacgca agcttatcga atgggaccgg gattacctca acatggatat
     4981 caatgcggtc gagcagcggt cctgaaatgc gatttaggta cttttgcaca actccgggcg
     5041 aacacacgca ttccttttca ggatgattgt agtaaccgca tgggcaggga ttcatggagg
     5101 caactaacat gaagctggcg ggatactcaa ccgtaaactt tgcccgcgat atggttataa
     5161 ccctgtcctc aaggggttgg cgcataacct ctaaaactgt tcttttaaac tcgggtagct
     5221 catcgagaaa aagtacgccg ttatgggcga gcgatatttc accgggctgt ggaaactgcc
     5281 caccaccaac aagagcaatg tcggagatgg tatggtgtgg actacggaat ggcctacggg
     5341 tcatcaatga tgtattcctg tcaattttac ctgcaacgga atgaattttg gtggtctcaa
     5401 gcgcctcgcg caatgttaac gggggaataa ttgttggtat ccgcttggcc agcatggttt
     5461 ttcctgcacc aggcgggcct atcataatga tgttgtgtcc tcctgcagca gccacctcaa
     5521 gcgcccgctt aacgttttcc tgccccttta catcggaaaa atcaacatcg tagttgttgg
     5581 cgtgggcaaa aaactcctcg cgggtgttca caatggttgg ctcaagagtg ctattgccat
     5641 taaaaaagtc aaccacctgt ttgatactct caaccccgta aacgtcgagg ttgtttacaa
     5701 cggcagcctc acgggcattc tgcgcgggaa caataaaacc tttaaaaccc tcctcgcggg
     5761 cctgaatggc tatgggcaaa acccctttaa tgggctggat ggtgccatcg agcgaaagct
     5821 cacccatgat aacgtaatcc ggcagcaaat cgggtttaat ctgttcagat gcagccataa
     5881 ttccaatggc cagtggtaaa tcgtaagccg agccctcctt gcggatgtcg gccggagcca
     5941 tgttgattac gattctcttt ttgggaaact tgtagccatt agtctctagt gccgacataa
     6001 cacgctgttg gctctcctta acagcgctat cgggtaaccc aacaaggaaa aagtttaccc
     6061 cttgcccaac acttacctca atggtaattg ttgtggcttt tatgccatga accgcactac
     6121 caaaggtctt tacaagcata actcgataaa aaaccagaat agtatggcct attccggtaa
     6181 tttcattaat ggcataccga taaggttctt gcctcttgca tcagcttgcg ggttaacttg
     6241 cttagctcaa cctttgcatt cattagctgg tcaggacttg cagcttcaca aagaacctca
     6301 atggcttgat tttcggcatc attacaccca tcggggtcat ctggagtagc ttttgtctta
     6361 tcgcagtaat atttgctatc gcctacctcg tagtaacagt tcccgccatc ggttgggcaa
     6421 caaacgttgg ctatttcaca ctggggtggc tcaaatattt ggcaaacatc ctcttcttcc
     6481 ttttcgcaac cccaggttag gaatgccagt accgctccta gaataattgg ggcaaccagt
     6541 aacttctttc gcataacttt attggtttaa gtgtttaact atgatagatt taaactgctc
     6601 aaagggcata taacccctaa aaactctacc ctcaataaca aatgtaggtg ttccggtaaa
     6661 tccaaattta tcaaattcgt tccggatttg caaaatataa tccatcgatt cattccctag
     6721 aatgcactcg gcaaccagtg ggtcgctatc aatgaaatgg ttgaccattt cacggaactc
     6781 gtcactatat atcacgctac tatttgtaac tagcagctca tgcaacttcc cataataccc
     6841 gtatcggttt atacatactg ctgttttcac ctcatttatc cggtcagcgt tatttgagtg
     6901 ctccactatt ttcagaacca agcgtacctt tccggtggaa atgaattccc tttcaagctc
     6961 aggataaacc tcagtaaaaa aaagcctgca gtaaccgcaa tggtaactta tgtatgcgta
     7021 aatggcaagc tgagccgaat cgcttccaaa cgtgatatct aaaggattgg atgaaatagg
     7081 tgcggctagc ttactagtct tatttggggc caactgccac gcttttatgc caattaaaac
     7141 tataagtaca atggaaatcg ctactgcaat ccactcaatt ggtcttaact tccttcccat
     7201 cctttttttc atcagctgtt ttccgtttgg attcctttct gtccaattcg gtactaaagt
     7261 tcattacatg ggggtgaaaa accaaattaa gcccgcgttc ctgaacgcga atggtggcct
     7321 tcatggttgc ctgtaactcc ttagtatcaa acccatattt cgattctttt cctctttcaa
     7381 acgctacaag gtatagccct gagcgtttag ccttgtttgg cccttcaaat ccctcgtcct
     7441 tcaaaccctg ctttttcaaa agctttttgg attttccaag cattacatca ccggggtaaa
     7501 ccacaaagtg atactcgtag tcatcggcaa agctttgcat accgtaaacc gacatatcca
     7561 tggcggtact tacgatgttg gtttttggga agtaaatctg gttgttgtaa ataaacacac
     7621 tggtttccag tgtattaaat acaatattgt ttaactcatt aaggttagta aactttgaca
     7681 gctccatggc aggctcaaag ttgtatatac caccattttc aagcttaaag ttgcctaaaa
     7741 ggttcatctt gtcataaagc aggttggtgt cctgcatcac aattcgccca aaaaccgtac
     7801 ctgtaactaa tccatcgata tttttatggg tgatataatc ctgctcaaaa tcgtcgtttt
     7861 gttctaacaa ctgcttgata tttaacctat tcgattcatt cttgaattca atgatttcca
     7921 gtggatactt acgggtgtcg taaaccaacg aggtaaccat attgccccca aatgctttca
     7981 ttcgtagttc gtcaagcaca tagaggctat cgtcaaccct gaactttgtc gaaaggtcgt
     8041 caattagtac tttcccgtat ttcaccttat cggctttggc aatgccccta ataatgtatt
     8101 gtgggatagt tgaggcagcc gtatcggggc ttgccgttga gtcctcggcc ataaagggta
     8161 ggaaaatatc gtaatcaaca tagttagccc aaagcgatgt attaacaatg agctttttat
     8221 ccttttgccc taaaatgaat gttttataaa cattccagat agaggttgaa tcggagcgaa
     8281 agtccagatt tttgtagtgt ccattcacat tggctaaacg aatggtatcg tttgccattg
     8341 aaagggtaag cgataaccta tcgacgccaa tcaggctatc gttgggcaat gagaaaccaa
     8401 aatccttgat ttgcgcagca aacatgctgt tttcaaatag gattggcatt atctgcttat
     8461 caatagaatc ggggttaatt gttcccgatg tgctaaatcc aaaatctatc ttaccggtaa
     8521 caaggctaac aagcgtatca ggaataaatc ggtgtagctc atccaaatcg acatacaaac
     8581 ggccttcgcc cctgaaaacg gggcttttca agtttttgaa cttcatctta ctgctaaaac
     8641 tactattgcc catggcaata tgaagcgtat cgacccttac atcaatttta tcaagttccg
     8701 atatacgccc tatgaccttt actttaagta aaccatcctt taaacttacc ttgtaggcag
     8761 gcaaacgtaa tgagaggttg ttaaccgtta gttccttgcc agggctatat gttacctcgg
     8821 catttaattc ctttacagca ttaaccgtgt ctaccccggc attgatattg ctaaaagata
     8881 aggaaaatcc cgtgttgtcc ataaagtaat cggagctgtt cacatcaagg tattcgggca
     8941 ttttaccctt ggtaaaaaat ttagcatcaa ttaaaccgga aagttctgtc agagtgctat
     9001 cgggtagcaa tggggcaaac tcgtcgagga caagatgcat gtcgccttga atgttgtaaa
     9061 gtggtttgtt tggatttttc agcgtaagag ttaggttagc cctactttgc tgagtttcaa
     9121 gattgaattt tttaatattt actgctgatg tggcaaggta gttcgggttt gggattgaaa
     9181 cgcttccctc gaagtcaagc ttatttactt ttgggtattg tttagtttca actacacctt
     9241 tcctcaagtc aaaatcaaca aaaacgcttg gaagcattag ggtatcgtat aaataccccc
     9301 taacaattgt gttaaactta agggttcccc ctagcctcga caccccatac tccttaatca
     9361 tactctcagg aataaatcca taaagctcag caaggttaag ttttggcaaa gcaaggctaa
     9421 aatccaccca gattgaatcc gacagggttg cgttaccagt ggctttaagt tctatgccat
     9481 ttatatcgaa cttggcatag tcgagtaaaa ggtttccatt gtcgtaatca acactgtagg
     9541 aaaagttgag gtttgtaacc ttctccagtg gtaaatcggc aaaccgggta ttggttacaa
     9601 caatttcgcc attacttttc ccccagatac tatcgcccga aacccttccc tcaaggtgaa
     9661 gtttaggaat aacaatatcc gctgcagcct ttagcttttg gtcggaatat gtcaaatgaa
     9721 tatcgctaag ggtaagattc tttaaaagaa tgtttagaat ggtttgtgta actgtatcta
     9781 caggagtttc aactgtatcg ggaggtatca aaaaatcaaa attcgatttt ccagtagaat
     9841 caaccaggta gtttacccta aatccattca gctcaacccc gttaattatt accttgtctt
     9901 tgagtagtgg atatgccttt acggaaacaa aaacgttttt aaacccaacc agggtatcag
     9961 gtaaactccc tataccaaat acccgcaccg agtcggcctg ctgacctagt ttaaagtcgg
    10021 caatacgaac agtaagattc gggaaatccc gaaaaggaac taacgaaacc tccccgtagg
    10081 agataggcgc ttttagattt gattcaatct ccttaaccgc aagacgaata atgcttccct
    10141 caaacagctt tgcaatccca ataataatga ataccagtaa gtgtaaaaag ataatagtta
    10201 ggccaaaata ctttaacaac cttacaataa aacgctttgt cttcataccc tgttatcgga
    10261 attattagtt tttgcaatcg gagttactaa aaaacttcca tttccggtac ttacgcaatg
    10321 tattgctgag ctcagaacct cggaggttct ttgcaataac tacgcccgaa ctgtccacca
    10381 gaaagttcat aggaattgca ttgacactgt acgcttttgc cgcctcgctc cgccaacctt
    10441 ttaggtcgct tatatggtaa ggccatacta gtttatcgtc ggctattgcg tttacccagt
    10501 tatcacgttt taggtcaagc gatacgctga atatcgcaaa tccatttcca ttggtaaagc
    10561 aggcatcctt gtacttattg taaacctcag caagtacagg gttttccttt ctacagggcg
    10621 agcaccacga tgcccaaaaa tcaaccagca ccagcttccc ccttaacgat gttaacttaa
    10681 actcctcacc attgggacca taccctgtga tatcagggac aacctgtcca acagatatct
    10741 cctgcgaata accacttagg cataaaacca ttatgctgac aaccattagg tattttacgc
    10801 taattctcat gcttggcttt ttattctttt tcaattcaaa tttcatgcaa tttatggaat
    10861 tttaaagaga ttttgaatat gaaacgaaga aataaatcgt tagggttagt tttgagccaa
    10921 agggaaggct aatggaacgc agatgatata gttgttcgtt gattgttact cgttgtttgt
    10981 gagatggaat ggaacgctga tgacacagat aatactgatt ttcactgtga aaccctgggg
    11041 tgcttattta gttgcctgtt gatagttgat agtttgaata tgacccgttc cctattgtca
    11101 tgttgaactc ccgatatatc gggatagaat ccgccgaagc atcacttttt gccttcaacc
    11161 cttaatgaga ttcctctcat ttgctcagaa ttacaaaaaa gttgttcgtt gattatgaat
    11221 tagaagaatg cacgcgtcat gagacgcgcg ctaacgtgtt aaacgaacct tgaacttgaa
    11281 accttgaacc tcgaccttga atgccgttta acagcacaag ctctttatcc tttaaccttt
    11341 aacctttatc cttccttaga acctattcaa ccagcggtag cggcgggcgg tatcgcctag
    11401 tttcatacct agcatcagct cgtgcgatcc tgctttggag aatgtcgata catcggtaaa
    11461 gttatagtca aaggcgtagc caaaatagaa ctgctggtat tttacaccaa tcatggtaat
    11521 aatagcaccg gtggaacgta ttgaaacgcc agcccaataa tcgtttttat agtacccctt
    11581 aaggttgagg tctgctccaa actgaaaacg ctcattggtg gttaatagaa ccgaaggttc
    11641 aatggcaaaa tcgctgcggt agggttggta cctatacccc ccaaccaggt agtactggcg
    11701 aagtaaacgg tatgccacct cggaattacc tccaccaaac tgaataaaac tctggaatag
    11761 cgaagtagcc gagaaaccca cgtaatactt ttgtgtgcta agtaacaccc ccaaattggc
    11821 atcgggagcg aatttggagg tcttacccat tagcaatggg tcgttggttt gttcgtatcc
    11881 atcaatggca tttacatttg ccttgaactg aaagctggta agcgaagcgc cgaagctcag
    11941 ctgttgatcg cgcatgtaga tatggtaggc gtaagtagcc tgaaatccgg tacgatgtat
    12001 tcggccattg ttatcgttaa aaacaaatgc tccaaggcca accctaccgc tagggcgacg
    12061 cctgcgtaca cgggttttta taagacggct cctgttgcgg aagctggttt ttagtatacg
    12121 ggtctgagcg cttaaggcat aggtactagg agcctcagag tagccaatcc attgctgacg
    12181 tgctgttagg ttaacagcgg ttacaccctc ggctcctgcc acggcaggat ttaccaggaa
    12241 gggatcaagc atgtactggc tgtatagggg ttgctgctgg ccttttaccg cacctgccac
    12301 tcccatcaga gccataacag caactattcc tatgtgtttg tttcttctca ttaatacttg
    12361 tttatattcc agattcaaga tttcaaattt caaaaattgc tccgcttgaa gtttaaggtt
    12421 taaggtttaa ggtttaaggt taaaatatgt gtcgtaaatt tattattttt actatcaacc
    12481 atcaactatc aactatcaac catcaactac ctaataatcg taacgctacc tttgtactcg
    12541 tacttctttt cgccaacggt gaatacaatg atgtagtgat acgaatccat gggtaactcc
    12601 ttaccgttca aatcctttcc atcccagggt ttgtatgcac ccttgctgct ccataccatt
    12661 ttaccccaac ggttgaatac tttcacgtca acattggtta tataacctcg caggtcttgc
    12721 cccttgtagg gagctgggaa ctcccaggta tcgtttacgc catccttatt gggcgtaaag
    12781 gctgcaggaa tgcgcaggtt gctgtaatcg gcagccgaaa cctgaatggt atcggaattg
    12841 gaacaaccat agttatcggt tacctttacc caaatggttt ggtcgccact ttggccatca
    12901 accgtaatgc ttggggtaat ggcaccggtg ctccagtcgt aaaccgtaaa atcggggttg
    12961 tacacatcga gcagtagggt ttggttggca aacagggtag tatcgttacc taaatcaacc
    13021 accggaagtg gcctaacggt tacctcggtt atatcgtagg aacagctgta gtttgagtta
    13081 accagcctaa cggtgtaggt tccgctctcg ccggtatagt aaacctggtt agtatttcca
    13141 tc
//
LOCUS       DAOSAL010000073        13062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_3226_length_13062_cov_51.323595, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000073 DAOSAL010000000
VERSION     DAOSAL010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 13062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13062
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3226_length_13062_cov_51.323595"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(240..899)
                     /locus_tag="PK049_09465"
     CDS             complement(240..899)
                     /locus_tag="PK049_09465"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOK61826.1"
                     /translation="MSISSNTKPDNLIIRNIILDLGGVVLDIDYSLTLKAFENMGVYL
                     GNHTTLTGSNELFNQFDCGLINPDTFRQEFNQMFMVALSPKEFDKAWNALLLSWDTER
                     LKLIERLKKNYRVYLLSNTNVIHFNHYNRLLVEETGKELKDYFHKAYLSFEMGIRKPQ
                     VQIFQRVLSENGLIPSETLFVDDTHEHIDAAKGLGIRTIHLKGGNMAKPLAEALKGLV
                     G"
     gene            983..1056
                     /locus_tag="PK049_09470"
     tRNA            983..1056
                     /locus_tag="PK049_09470"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1017..1019,aa:Ile,seq:cat)
     gene            1210..2172
                     /locus_tag="PK049_09475"
     CDS             1210..2172
                     /locus_tag="PK049_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOK61827.1"
                     /translation="MKNILVVGAGGQIGSELVPHLQKIYGTNNVVAADINTSCAHHFQ
                     NGPFEVLDALDAQKFAELVRKYRVDTIFNLVALLSATGEKNPQLAWKINMGALMNSLE
                     IARENHCAVFTPSSIGAFGPTSPKDNTPQDTVMRPTTVYGICKVTGEMLSDYYFLKYG
                     VDTRSVRFPGIISNVTLPGGGTTDYAVEIYYGAIQQKHYVCPLPQGTYLDMMYMPDAL
                     DACVQLMEADPSKLKHRNSFNVTAMSFEPSQIYATIKKRIPDFAMEYNIDPVKKAIAD
                     SWPNYMDDSCAREEWGWNPKWNLETMTDDMLKVIGEKYKAGLLK"
     gene            complement(2216..2920)
                     /locus_tag="PK049_09480"
     CDS             complement(2216..2920)
                     /locus_tag="PK049_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOK61828.1"
                     /translation="MVLKSIILSLLLLLPLWVLPQIIIEKPFAAELSHPELSIEQIAI
                     YPDSTVVSLTVVNNADSNGWFCADKNIYIENLNTRQRYYRIGSHGIPTCPTTHNFSRK
                     GETLSFELIFPPIPKNLNSINLIEDCNKACFFFKGIILSNKLNSDIRLFNLGMEYYTK
                     NDIAQAVECFSKVVEEIPANPTHVYGYAYYHLVMIFYTKGDKLTASFWLNQLKTSALP
                     NKDYFVRMVKRDTGME"
     gene            complement(2984..4603)
                     /locus_tag="PK049_09485"
     CDS             complement(2984..4603)
                     /locus_tag="PK049_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic hydrocarbon degradation protein"
                     /protein_id="HOK61829.1"
                     /translation="MRKQLLTIIATGLTTLVMAGGIVTNTNQSATYVRMLARDASTSI
                     DAVYFNPAGLVKMENGFHLYLSNQTIFQKKTIENKFPLLNESKYVGDVAAPLFPDFYA
                     VYKKDKLALGFGIQPNAGGGSAKYNTGLPSFEIPVSVIPASLTANGITTTKYSADINF
                     DGSSVFWGAQINASYAINDMISLSLGARAILAKNTYNGFLKNILINPNQPAFGANYNG
                     ANMVPATQFFTDAANTLNAWSTGATSYYNGLEPIVNGGGGSTLLVNGTSVGLTSDQIT
                     QISGLLVAAGVPQSQIGSIDIQTAQATLGAAAPVFAGKSAAMAANATATADKEVDAIQ
                     TGTGYTPIIGANLTFGDKLNIGIKYEFRTKLTLTNDTKVDGTGLFPDGGKLRYDIPAI
                     LSAGFEYKILQSLRFASGFHYYFDKDAVIESAPGVKKDIEGNLYEIAAGLEYDITDKI
                     LVSAGYLYAKTGVGKGFQTDLTHSLTSNTVGFGGMFKVNDNLSLELAGLFTIYNSDSK
                     EIDYSAYGVPSAIETYNRTNVLFSIGAAYKF"
     gene            complement(4719..4961)
                     /locus_tag="PK049_09490"
     CDS             complement(4719..4961)
                     /locus_tag="PK049_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61830.1"
                     /translation="MRRAKIYLAIVAYFTLVKRFLSKPLYRFVMRIKQKLGIKSVTRC
                     SYDGRVLPAGFEFFFNGGSHLNLGLEQGFLNQYLRN"
     gene            5104..6252
                     /locus_tag="PK049_09495"
     CDS             5104..6252
                     /locus_tag="PK049_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010801286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOK61831.1"
                     /translation="MYKKLILPFLLMLCQAVNAQTDSFQFTVKYEIPTSPIKDQQRTG
                     TCWSFATTSFVETELIRMGQPVFDISEMYFVRYAYEGKAQNYVRFQGANNFGQGGQAH
                     DVMNVIRKYGMVTEKEYPGLNYGSEVHAHAELEAVLKAFLDAVVKNPNKKLSTAWFPA
                     YRGILDAYLGAVPDKGNSPLSKVPFNPDDYIEFTSFNHHPFYKRVSLEIPDNWSHDLY
                     YNLPLDEMMQVITYALGNGYSVCWDGDVSEKSFAYRKGVALLPVSKFEELSGSDKERW
                     TGITEKDFTARMANFDAPVPEIKVTQENRQANFDNLTSTDDHLMHLTGMATDQNGTVY
                     FLTKNSWGTEGVYDGYLYMSESYVRMKTIAVMVHKNAIPAEIKKKLAL"
     gene            complement(6254..7234)
                     /locus_tag="PK049_09500"
     CDS             complement(6254..7234)
                     /locus_tag="PK049_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOK61832.1"
                     /translation="MDVEKIFNISSDAEFENLALEVFRFQYKNVDIYRTYVDLLKVDP
                     NKVMEIYQIPFLPIQFFKSQQILANGLSPEIEFTSSGTTGSETSRHYVAKVDIYKQSY
                     TLGFRHFYGSEQQYCILALLPAYLERTGSSLVYMVDGLIRQSGNPHGGFYLNQLNELA
                     AKLQMLDESGQKCLLIGVSFALLDLAEIHSLRLKNTIVMETGGMKGRRREMVRDELHG
                     ILTAAFGVDSIHSEYGMTELLSQAYSTGKGVFSCPPWMRVLIRETNDPLTIAPVGATG
                     GINIIDFANLYSCAFIQTQDLGRLNHDGTFEVLGRMDSAPIRGCNLLVYG"
     gene            complement(7450..7812)
                     /locus_tag="PK049_09505"
     CDS             complement(7450..7812)
                     /locus_tag="PK049_09505"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HOK61833.1"
                     /translation="MSQTSRNNDNFFERVYQVTRLVPFGRVTTYGAIARYLGATRSAR
                     MVGWALNSSSSQEEWVPAHRVVNRKGLLTGKIHFPGSNTMQQLLEAEGIRVEDNAVVD
                     FDKYFWDPNVELVKGSKK"
     gene            complement(7784..8494)
                     /gene="trmB"
                     /locus_tag="PK049_09510"
     CDS             complement(7784..8494)
                     /gene="trmB"
                     /locus_tag="PK049_09510"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOK61834.1"
                     /translation="MGKNKLRRFAENETFENLFQPSFQEVFNTDYRLKGQWQSGFFKN
                     QNPIVLELGCGKGEYTVGLARMFPDKNFIGIDIKGARMWRGAKTALEEGLKNVAFIRT
                     RIEHIKSFFSQDEVDEIWVTFPDPQPRPSKATKRLTSSRFLNYYIQFLKPNAIVHLKT
                     DSQPLHAYTKALVEQNNLKMLVCTSNLYSEVSNDPILGIKTFYEQQFLDQGLPITYIK
                     FQVNHSGPFHEPNIEEQR"
     gene            complement(8509..8985)
                     /locus_tag="PK049_09515"
     CDS             complement(8509..8985)
                     /locus_tag="PK049_09515"
                     /inference="COORDINATES: protein motif:HMM:NF025474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HOK61835.1"
                     /translation="MTKLKLLVISVITIALFAACSDNYVYNGYVALPQEQWHADSIAS
                     FRIPVSDTMTYYNLYVNVRNTTDYPFQNLYLFIDIAAPNGAIARDTFECYLADDHGKW
                     LGKGRGKYRDNRFTYRQNVRFGAEGEYRITLQQAMRVEQLKGISEIGFRVELGSNK"
     gene            complement(9131..10093)
                     /gene="ricT"
                     /locus_tag="PK049_09520"
                     /pseudo
     CDS             complement(9131..10093)
                     /gene="ricT"
                     /locus_tag="PK049_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
     gene            complement(10188..11336)
                     /gene="holB"
                     /locus_tag="PK049_09525"
     CDS             complement(10188..11336)
                     /gene="holB"
                     /locus_tag="PK049_09525"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HOK61836.1"
                     /translation="MFFSDIVGQEQVKERLLLQVKENRVPHAMLLAGPPGTGKLALAV
                     AFAQYLMCTNKNENDSCGVCPACKKVSKLIHPDLHFVYPGKAGKEKSDTADGATEAGT
                     DVLAIWRKMLLENPYMSETDWYDAIDLNNKQGMINVATASEVIRKLSLKSYESDVKVM
                     IIWLAERMNVQASNKLLKLIEEPPAKTFFILVSENTNLILKTILSRTQQVYVPPLTKS
                     EIAVVLTEKHGIAPNRAGDIARISNGSFYEALRLVNQNTGEYFEQYRNLMRLCYAKRY
                     IDLLDWVDDISRLGREEQKEFLSYSLKLTRESLMLNMGLDDITYLMGDEADFGKKFSP
                     FLGPANVNQITKAFSHAYECIAQNGNPQITFADMVFSLSKHIGPNPKT"
     gene            11518..12780
                     /locus_tag="PK049_09530"
     CDS             11518..12780
                     /locus_tag="PK049_09530"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOK61837.1"
                     /translation="MISIDSYSFKGKKAIVRVDFNVPLDKKTFEVTDDTRIRGAMPTI
                     KKILNDGGAAILMSHLGRPDGKPQDKYSLKHVIPAIEKNLGKSIKFAPDCVGEEAKKL
                     AAELKAGEVLLLENLRFYEEEEGKPYSLAEDASEEQKKEAKARVKESQKEFVKKLASL
                     ADCYVNDAFGTAHRAHASTALIAAHFPGNSMFGYLIESELKAMDKVLKAPEKPFTAIM
                     GGAKVSDKILIIENLLERVDNLIIGGGMTYTFIKAQGGEIGKSICEDDKLELARELLS
                     KAKAKGVNVYLPVDAVNADAFDANANTNISKIDQTPAGWMGLDIGPETVKLFSDVILK
                     SKTILWNGPMGVFEMDKFAQGTSEIAKAIAEATAKGAFSLVGGGDSVAAVNKNKLADK
                     ISYVSTGGGAMLEYMEGKELPGIKAIRG"
BASE COUNT         3622 a         2767 c         2838 g         3835 t
ORIGIN      
        1 gcccctacgg ggctggaaac gttgttcgtt gaccgtgaat cgttgttcgt gaatatcacg
       61 ccacacgttc tgtgaaacac catgtaaact ctgtgtaatc ctgtgatacc aatttagttg
      121 atggttgttg gttgatagtt gatagtttat aatagattac ctaaaatctt gaatctgaaa
      181 tcttaaatct gaaattttaa atttttatcc tttatccttt aacctttaac cttgccattt
      241 cagcccacta gccccttaag cgcctcagca agcggtttag ccatatttcc gcctttcagg
      301 tggattgtac gtatccctaa ccctttagca gcatcgatgt gctcatgggt atcgtcaaca
      361 aaaagtgttt ctgaaggtat taagccgttc tcgcttaaca cacgctgaaa gatttgaact
      421 tgtggtttcc tgatgcccat ttcaaaggag aggtatgcct tgtggaagta atccttaagc
      481 tctttgcctg tttcctcaac aagcagcctg ttgtagtggt taaaatggat aacgtttgtg
      541 ttgcttagca ggtataccct ataattcttt tttaatcgtt caattaattt gagcctttcg
      601 gtatcccaac tcagaagcaa agcgttccat gctttatcga actccttggg gctgagagca
      661 accataaaca tttggttgaa ctcctgccta aaggtatcag ggttaatcaa accgcaatcg
      721 aactggttga aaagctcgtt acttcctgta agtgtagtat gattgcccag gtaaacaccc
      781 atgttttcaa aggcttttag ggtaaggcta taatcaatat caagtaccac tccacctaaa
      841 tcaagtatta tatttctgat aatcagattg tcaggttttg tattgctgct aatactcata
      901 ttttttggac tccctatttg aaaattcaaa tgcaaatatg taattttgca aaccaaaata
      961 aaaacagtaa aaactgtttt ttgggcccat agctcagcag gttagagcat ctgactcata
     1021 atcagagggt cgctggttcg agcccagctg ggcccacaaa ccgccgcaag gcggtttttt
     1081 tttattcact ccatcaaaaa atctgcttaa aaatataaat ttaaacgtgg gttttgggtt
     1141 tgaagtgtta accaaatact atacatttgc gaaaaatcat aagtataatc accttctaaa
     1201 aataaaagca tgaaaaacat attggttgtt ggtgccggtg ggcaaattgg ttcagagctg
     1261 gtgccgcatc tccaaaagat atatggaacc aacaatgttg tggctgccga tatcaacaca
     1321 agctgcgccc atcactttca gaatggtccg tttgaggtgc tcgatgccct tgatgcccaa
     1381 aaatttgctg aattggtgcg gaagtatagg gttgatacta ttttcaacct ggttgcatta
     1441 ctttctgcta ctggcgaaaa aaatccgcag ctggcctgga agataaacat gggagcactg
     1501 atgaactcgc ttgagattgc tcgcgagaac cattgtgctg tgtttacccc cagttccatt
     1561 ggcgcttttg gcccaacatc gccaaaggac aacacccctc aggataccgt tatgcgccca
     1621 actactgttt acggtatttg taaggttaca ggcgaaatgc tgagcgatta ctacttccta
     1681 aagtatggag tagatacccg cagtgtacgt ttccccggaa ttatttcaaa tgttactctc
     1741 cctggtggtg gtactaccga ttacgcagta gagatttact atggagcaat tcagcagaag
     1801 cattacgttt gcccattgcc ccaaggtaca tatctcgata tgatgtacat gcccgatgcc
     1861 cttgatgctt gcgttcagct catggaggca gatccatcaa agctcaagca ccgtaacagc
     1921 tttaacgtaa ctgcaatgag ttttgagccc tcacaaattt atgctaccat aaaaaagcgc
     1981 atccccgatt ttgccatgga gtacaatatc gaccctgtaa agaaagccat agctgatagc
     2041 tggcccaact acatggacga tagttgcgcc cgtgaggagt ggggatggaa ccccaagtgg
     2101 aacttggaaa ccatgaccga cgatatgctt aaggtgattg gagaaaagta taaggcaggt
     2161 ttacttaaat agctttagta taatgataca aagggaggtg ttgagcctcc tttttttatt
     2221 ccattcccgt atcgcgctta accattcgaa cgaagtagtc cttgttagga agagctgatg
     2281 tttttagttg gttcagccag aagctagcag tgagcttatc gcccttagta tagaaaatca
     2341 ttaccaaatg gtagtaggcg tacccataaa catgggtagg attagcaggt atttcctcaa
     2401 ctaccttgct gaaacattca actgcttgtg caatatcgtt tttggtgtaa tactccatgc
     2461 ccaggttgaa taaccttata tcggagttta acttattgct tagtattatg cccttaaaaa
     2521 agaaacaggc tttattgcaa tcctcaataa ggttgatact gttgaggttt tttggaatcg
     2581 gaggaaaaat aagttcaaac gatagagttt cgcccttacg gctaaagtta tgggttgtag
     2641 gacatgttgg aataccgtgt gagccaatcc tgtaataccg ttgtcgagta ttcaggtttt
     2701 caatgtagat atttttatcg gcacaaaacc agccattgct atcggcgtta ttcacaaccg
     2761 taagacttac caccgttgaa tccggataaa tggcaatctg ttcaatggaa agctctgggt
     2821 gcgagagttc agccgcaaag ggtttttcaa taatgatttg cggtaaaacc cataaaggga
     2881 gcaaaagtaa aagtgatagg atgattgatt ttagaaccat atagaaaaaa tttaggggtt
     2941 gagaaccaac ccctaaatta gtataattaa ttaacattta gaattagaac ttataggcag
     3001 cacctatcga gaataaaaca ttggtcctat tataggtttc aatagctgag ggaaccccat
     3061 aagcagaata atcgatttct tttgaatcag aattatatat ggtgaacaat cctgcaagtt
     3121 caagcgatag gttatcgttt actttgaaca tgccaccaaa gccaacggtg ttagatgtta
     3181 agctatgggt taaatcggtt tgaaagcctt tacccacacc tgtttttgcg taaaggtaac
     3241 cagctgaaac cagtatttta tcagtaatat catattctaa acctgcagca atttcgtaaa
     3301 gattaccttc gatatccttt ttaacgcctg gggcgctttc aataactgcg tctttatcaa
     3361 agtagtaatg aaatccgctt gcaaagcgca aggattgtaa aattttatat tcaaaacctg
     3421 ccgataggat agcaggaata tcgtaacgaa gttttcctcc atcggggaat aggccagtac
     3481 catcaacctt tgtgtcgttg gtcagtgtta gttttgttcg gaattcatat ttaataccaa
     3541 tgttaagttt atcgccaaaa gtcaagttag caccaattat aggagtataa cctgttccag
     3601 tttgaatagc atcaacctcc ttgtcagcag tagctgtagc atttgctgcc atggctgcag
     3661 atttaccagc aaatacaggg gcggcagcgc ccagagttgc ttgagctgtc tgaatatcaa
     3721 ttgaaccaat ttgcgattga ggaacacctg cagctacaag gagtccagaa atttgagtga
     3781 tttgatcaga tgttaatcca acacttgtcc cattcactag taatgttgaa cctcctccac
     3841 cattaactat tggttctagt ccattgtagt aactggttgc accggtagac cacgcattaa
     3901 gggtgtttgc tgcatcagta aaaaattgtg tagcgggaac catattagcg ccattgtaat
     3961 ttgcaccaaa agctggttgg ttaggattta ttagaatgtt tttaagaaag ccattatagg
     4021 tgtttttggc caaaattgct cgtgcaccaa gtgagagtga aatcatatcg tttatagcgt
     4081 aactggcatt aatttgtgca ccccaaaaaa cacttgaccc atcaaagtta atatctgcag
     4141 agtatttagt agtagtaatt ccattagctg ttaggcttgc aggtattaca gatactggga
     4201 tttcaaagct tggtaatcct gtattatact ttgcagagcc gcctcctgca tttggttgaa
     4261 tgccaaaacc taaagctagt ttatctttct tgtaaactgc ataaaaatca gggaataatg
     4321 gcgctgcaac gtctccaaca tatttggatt catttaaaag cggaaatttg ttttcgatag
     4381 tttttttctg aaaaatggtt tggttactca ggtagagatg aaaaccattt tccattttta
     4441 ctaaccccgc agggttaaaa taaacagcat cgatactggt tgatgcatcg cgagcaagca
     4501 ttcgcacgta ggttgcactt tggtttgtgt tggttacaat accaccggcc attaccagcg
     4561 tggttaaacc ggttgcaata atggttagta gttgttttct catacttctc attgtttagg
     4621 ttagtaatag attatattct gatttaaacg tttgtattga acgatggttt gaaacggaac
     4681 gaaaaatgtt cccctaatga taggttaaac gatagaaatc aattccttag gtactgattt
     4741 aaaaagcctt gctcaaggcc aaggttgagg tggctacctc cattaaagaa aaattcaaat
     4801 ccagccggta aaaccctgcc atcgtatgaa caacgggtga ccgactttat gccaagtttt
     4861 tgtttgatgc gcataacaaa cctatagagc ggtttgctta agaaccgctt tactagagta
     4921 aagtatgcaa caattgccag ataaattttt gccctgcgca tcgagtaggg tgaatatttg
     4981 gtgctaatat agataattat cgatatattt tcaactatca catctttttg taaaataatt
     5041 acatgaaaac gtttgttggt tttaattgct tttgcataac ttgcataaat aaatccgagt
     5101 tgtatgtata aaaagttaat tctgccattc ctgcttatgc tctgtcaggc agtaaatgca
     5161 caaaccgatt cttttcagtt tacggtaaag tatgagatac ctacttcgcc tataaaggat
     5221 cagcaacgta ctggcacctg ctggagcttt gcaaccacgt cgtttgttga aactgagcta
     5281 atacgaatgg gacagcccgt atttgatatc tccgagatgt attttgtgcg ctatgcctat
     5341 gagggtaagg cacagaacta tgttagattc cagggggcca acaattttgg tcagggtgga
     5401 caagcgcacg atgtaatgaa tgttatccgt aagtatggta tggtaaccga aaaagaatat
     5461 ccgggattga attacggctc cgaggttcac gcccatgctg agcttgaggc tgtgcttaaa
     5521 gcatttctgg atgctgtggt gaaaaaccca aacaaaaaat tgtcaaccgc ctggttccca
     5581 gcataccgtg gaattcttga tgcttacctt ggagctgtcc ccgataaagg aaattcacct
     5641 ttatcaaaag tcccctttaa tcctgacgat tacattgagt ttacctcatt caaccatcat
     5701 ccgttctaca aaagggtatc gcttgaaatt cccgataact ggtcgcacga cctttactac
     5761 aatctaccgc tcgatgagat gatgcaggta ataacctatg ccctggggaa tggctattcc
     5821 gtttgctggg atggcgatgt gagcgaaaaa agttttgcat accgcaaggg tgtggctctg
     5881 ttgcccgtat caaaatttga agagcttagt ggatccgata aggagcgatg gactggtatt
     5941 actgaaaagg attttaccgc acgcatggca aattttgacg cacctgttcc tgaaattaag
     6001 gttacgcagg aaaaccgcca ggcaaatttt gacaacctaa cctccaccga cgaccactta
     6061 atgcacctaa ccggtatggc caccgatcag aatggaacgg tttacttttt aaccaagaac
     6121 agctggggaa ctgagggggt gtacgatggc tatctataca tgtccgaatc gtatgtgcgt
     6181 atgaaaacta tagctgtgat ggttcacaaa aatgccattc ctgcggagat aaagaaaaag
     6241 ctagcattgt agcctacccg taaacaagta ggttgcagcc cctaatgggg gcagaatcca
     6301 tacggccaag cacctcaaat gttccatcgt gattgagtcg acctaaatcc tgcgtttgaa
     6361 taaaagcgca tgagtatagg tttgcaaaat cgattatgtt aattcctcca gtagctccta
     6421 caggcgctat ggtaagcgga tcgttagttt cacgaattaa aacacgcatc cagggtggac
     6481 agctgaaaac tccttttccc gttgagtagg cttgcgatag tagttcggtc atgccgtatt
     6541 cggaatggat actatcaacg ccaaatgctg cggtaagtat tccatgtaac tcatcgcgaa
     6601 ccatttccct acggcggcct ttcatgccgc cagtttccat aacaatagta ttcttaagcc
     6661 taaggctgtg tatctcggca aggtcaagca aggcaaatga tactccaatt aacaggcact
     6721 tttgaccgga ttcatcgagc atctgaagct ttgccgctag ctcgtttagc tggttgaggt
     6781 aaaaacctcc gtgcgggttg cccgactgcc tgataagccc atcgaccatg taaaccaacg
     6841 atgatccggt acgttccagg taagctggta gtagagctag tatgcagtat tgctgctcgc
     6901 taccatagaa gtggcgaaaa cccagggtgt agctttgttt gtaaatgtca accttagcaa
     6961 cataatgcct gctggtttcg gaacctgtgg ttccgctgct tgtaaactct atttcgggcg
     7021 aaagcccatt ggcaagaatt tgctggctct taaagaattg gatgggaagg aatggaatct
     7081 gatagatttc cataacctta ttggggtcaa cttttagtaa atcaacatag gttcggtaaa
     7141 tatcaacatt tttatactga aacctaaata cctcaagggc aaggttttca aattcagcat
     7201 cagagcttat attaaaaatc ttttctacgt ccattgccta taaatcgtga accgttaatt
     7261 gttaaacagg gtttggttta tatagctaga atgttgactt ccttaatcat gcaaacccga
     7321 atttttagag tttggtgggt catcccaaat ttcaaattta agattccaaa acccgtcccg
     7381 agttagcgag ttcaagtgcc tgctctggtt tatggagttc aagaacctga ccaaatatgc
     7441 gactttattc tattttttgc ttccttttac taactcaacg ttaggatccc agaagtactt
     7501 atcgaaatca accacagcat tatcttcaac ccttatgcct tccgcttcaa gcagttgctg
     7561 catggtattg ctaccgggga agtggatttt tccggttagc aaaccttttc ggtttaccac
     7621 cctgtgcgca ggtacccact cctcctggct cgatgatgag tttagcgccc aaccaaccat
     7681 tcgggctgaa cgggttgctc ccaggtatcg ggcaatggct ccataggtgg ttacacgacc
     7741 aaagggaacc aaccgggtta cctggtaaac ccgttcaaag aaattatcgt tgttcctcga
     7801 tgtttggctc atggaaaggg cctgaatggt ttacttgaaa tttgatatat gtaataggca
     7861 aaccttggtc aaggaattgc tgctcgtaaa aggttttaat gcctagaatg ggatcgttgc
     7921 ttacctcgga gtaaagattg ctggtgcaaa ccagcatttt aaggttattc tgctccacca
     7981 acgccttggt gtaggcatgt aagggttgac tatcggtttt aaggtgaact atggcgtttg
     8041 gttttaggaa ttgtatgtag taattcaaaa atcggctcga tgttaaccgc ttggttgcct
     8101 tcgatgggcg tggctgggga tcggggaagg tcacccatat ttcatcaacc tcgtcctggg
     8161 aaaagaatga cttaatatgc tcaatgcggg ttcggataaa agccacattc ttcaagccct
     8221 cctcaagggc tgtttttgca ccccgccaca ttcgggcccc ctttatatcg ataccaataa
     8281 aatttttatc agggaacatt cgggctaaac ccacggtgta ctcgcccttt ccgcatccaa
     8341 gttcaaggac aatagggttt tgatttttaa agaaacccga ttgccattgt ccctttaatc
     8401 tgtaatcggt gttgaaaacc tcctgaaacg aaggttggaa aaggttctca aaagtttcgt
     8461 tctcggcaaa acgacgtagc ttatttttcc ccacagtaaa atcaaacttt atttattact
     8521 tcccagctca accctaaatc caatttcgga gatacctttt aactgctcaa cccgcatggc
     8581 ttgctgcagg gtaatcctgt actcaccttc ggcaccaaac cttacatttt gcctgtaggt
     8641 aaagcggtta tcgcggtatt tacctcttcc tttccctaac catttgccat ggtcatcggc
     8701 aaggtaacat tcaaatgtgt cgcgtgcaat tgcgccattg ggcgcagcta tatctataaa
     8761 caggtaaagg ttttggaatg ggtaatcggt ggtattccta acgttcacgt aaaggttgta
     8821 gtaggtcatt gtatccgata ccggaattcg gaaactggca atgctatcgg catgccattg
     8881 ctcttgcggt aaagcaacgt atccgttgta tacatagtta tcggaacatg cggcaaatag
     8941 cgcaatggtt atgaccgaaa tgactaagag cttaagcttt gtcatcgttt ttatgtttgt
     9001 tttgtccttt gggtttgcga cgttttttca gtttgggttt cgattgctta tcgaagcggg
     9061 taatgctatc atcctccaag ccatcggtaa aatcgatatg ggtaaccgca gcttgggggg
     9121 caacattagt atcgagtagg ctgggcacct taatgccttt tcggtttagc tcaattatct
     9181 catttacacg ttccaccgga acctcaataa ggtttaccga tgacgatggc tctgagccaa
     9241 accacatact acgcttgaaa atatcggttt taaagtgata gtaagtggca tctttggttt
     9301 cgagctgtat gtttccttca ggtatgtcct tacgggcatc aacatagctt gcaagctcgt
     9361 agttcaagca gcacttcaac ttaccacatt ggccagctaa tttttggggg ttaagtgata
     9421 gctcctgtat ttttgctgca tttgtggtaa ccgaatcgaa ttgggcaatc caggtagagc
     9481 agcaaagctc gcggccgcat gtgccgatgc ctccaattct accagcttcc tgtcgggcac
     9541 caatctggcg catctcaaca cgaatcttaa attcctctgc aagaaccttg ataagctcac
     9601 ggaaatcgac acgttcatcg gcaatgtagt agaatatggc tttggtttta tcgccctgat
     9661 actcaacatc gccaatcttc atgtcgagct ttagcctttc ggctatcttg cgggagcgga
     9721 tcatagtatc gtgttcaagc gccatggctt cgacccactt ctcaatatcg gccttttttg
     9781 ccctgcggta tatctttttt agctccgctg ttttgggatc aacgcccatg cgcttcatct
     9841 ggcgctcaac cagccatcct gttagcgata ctacaccaat atcgtgaccg ggcgatgcct
     9901 caacggcaac aatatcgcct ttctgtaggc ttatctgatt tacattgcgg taaaacccct
     9961 tacgggtgtt cttgaactgt atctcaacaa tatcgtattc ctggagcgtt tccggaaggt
    10021 cctgaaccca atcgtaaaca ccaagtttgg agcagcaatt gccgcaggag ctatggtcag
    10081 taacctcgtt tatgtaaact gcgttgcccc ttggggtata tattttttcc atattcaata
    10141 tcaagctaaa ccttgcaaag ttaactaatt aaacgtaaaa ccaactacta tgttttaggg
    10201 ttgggtccaa tatgcttact caggctaaac accatatcgg caaaggtgat ttggggatta
    10261 ccgttttggg caatgcactc gtaggcatgg gaaaatgctt tggtgatctg gttaacattt
    10321 gctggaccca gaaaagggga gaactttttg ccaaaatcgg cttcgtcgcc catcaggtag
    10381 gtaatatcgt cgagccccat gttaagcatt aagctttcgc gggtaagctt tagggagtaa
    10441 cttaggaact ctttttgttc ctcgcgccca aggcgcgaga tatcatcaac ccagtcgagc
    10501 aaatcaatgt aacgcttggc gtaacatagc ctcatcaggt ttcggtactg ctcaaaatac
    10561 tctccggtgt tctggttaac caatctaaga gcctcataga aactaccatt ggaaattcgt
    10621 gcaatatccc cagccctatt aggtgctata ccatgtttct cagtaagaac aacggctatt
    10681 tcgcttttcg ttaggggagg cacatacacc tgttgggtgc gggaaaggat agttttgagg
    10741 atgaggtttg tgttttcgct aaccagtatg aaaaaggttt ttgcgggggg ctcctcaatg
    10801 agttttaaaa gcttattgga tgcctgcacg ttcatacgtt cagccagcca aataatcata
    10861 accttaacat cgctctcgta actttttagg ctaagcttgc gaattacctc tgaggcagta
    10921 gccacgttga tcatgccctg cttgttgtta aggtcaatag cgtcgtacca gtccgtttcg
    10981 ctcatgtagg ggttttccag taacatttta cgccaaatgg caagcacgtc ggtaccggct
    11041 tcggtagcac catcggcagt atcggatttc tccttgcctg cctttccggg gtatacaaaa
    11101 tgcaggtcgg ggtggataag tttcgaaacc tttttgcagg cggggcaaac cccgcacgaa
    11161 tcgttctcgt ttttgttagt acacattagg tattgtgcaa aggcaactgc tagcgcaagt
    11221 ttccctgtac ccggaggtcc tgcaagcagc attgcatgtg gcacacggtt ctcctttacc
    11281 tgcaatagca accgctcctt aacctgctcc tgcccaacta tatcgctgaa aaacatatcg
    11341 ttccaaatta ttggctaaac tccaaagtta acaattttat ggtatagtca acttatagtt
    11401 tgaaagcctt tcccaaggct ttgttttcaa ccgtttgcgt gaaaaatatt gatttagact
    11461 ctgcaattca cttcgaattt tgtatttttg cttcaaaccc actaaaaaca attcaatatg
    11521 ataagcattg acagttacag ctttaagggg aagaaagcca tagttagggt tgattttaat
    11581 gtgcccctgg ataagaaaac ctttgaggtt actgacgata cccgtattcg tggcgcaatg
    11641 ccaacaatca agaagattct gaacgacggt ggtgctgcca ttctgatgtc gcatcttggc
    11701 cgccccgatg ggaaaccaca agataagtat tcgttaaagc atgttattcc cgcaattgag
    11761 aaaaacctgg gtaagagcat caagtttgct cccgattgtg tgggcgagga agccaaaaag
    11821 cttgctgctg agctcaaggc cggagaggta ctattgctcg agaatcttcg tttttatgag
    11881 gaggaagagg gcaagcccta tagcttagcc gaagatgcta gcgaggaaca aaaaaaggaa
    11941 gccaaggcaa gggttaaaga gagccaaaag gagtttgtaa aaaaacttgc cagccttgcc
    12001 gattgctacg ttaacgatgc ctttggtact gcacaccgtg cccatgcttc cactgcgtta
    12061 attgctgctc atttcccagg caatagcatg ttcggttacc tgattgagag tgagttaaag
    12121 gctatggata aggttctcaa agctcctgaa aagccattca ctgccattat gggaggtgct
    12181 aaggtgtccg ataaaatttt gataattgag aatctgcttg agcgcgttga caacctgatt
    12241 attggtggtg gaatgaccta cacatttatc aaggctcagg gtggagaaat tggaaaatcc
    12301 atatgcgagg acgataagct tgagcttgct cgcgagctgc tttccaaggc caaggccaaa
    12361 ggggttaacg tgtacctgcc tgttgacgct gtaaatgccg atgcatttga tgcaaatgct
    12421 aataccaata tcagcaagat tgaccaaacc cctgcgggtt ggatggggct cgatataggt
    12481 cctgaaaccg ttaagctttt cagcgatgtt atcctaaaat ccaaaaccat actctggaat
    12541 ggccctatgg gtgtttttga gatggataag tttgcccagg gcacctcgga aattgctaag
    12601 gctattgccg aggctacagc aaaaggggca ttcagccttg ttggcggtgg cgattcagta
    12661 gccgcagtaa acaagaataa gcttgccgac aaaatatcgt acgtttcaac cggcggtggt
    12721 gccatgcttg agtatatgga aggtaaggaa ctcccaggta ttaaagccat cagaggctaa
    12781 cattactata aaaaccatat tgtgccctaa acagaaaaag tttagggcat ttttatctgt
    12841 atatttcata aaattgatat caatctagtt gaatttgttg attaatgcca tctcggcctc
    12901 gctttttgct ttttactttt tattttttta ttttttactt ttcctctaac ttcctctaac
    12961 ttcataatat tcattctact ccggcacatt cccacctaaa gtaacaacat gtctagtcct
    13021 gaaataagtc tacaggttag aaatagattt aagcgacaca ca
//
LOCUS       DAOSAL010000074        12134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_3529_length_12134_cov_51.969617, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000074 DAOSAL010000000
VERSION     DAOSAL010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 12134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12134
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3529_length_12134_cov_51.969617"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..1131)
                     /locus_tag="PK049_09535"
     CDS             complement(61..1131)
                     /locus_tag="PK049_09535"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOK61838.1"
                     /translation="MKKFLLVGLMFSIITQLSAQNKEDILRNLENPDTLKQQSAQTSS
                     DKQPMVIVRKIVKVVDGDSIIEERIDTVTSVNAVTVDSDNMAWVNTMGDTIKAAKGDT
                     VVVRLGKKKMVIVDSKDKTVIEIPEKGKKSDEIIEYKEVKKFKGHWSGFELGINGFMD
                     KNQSMTQTGDIAWMDLKQARSWNTNINFTQYSIGFGTDKAGLVTGLGLEFNDYHFSNP
                     ITLKVENGVTVVDSSYIQAGYKVEKTKITMSHLTLPLLLEFQIPMGEKRKDRIYISAG
                     VIGGVRLGSHTKVVYDDGSRHKDKNRGDFNIATLRYGLTARMGYKGIRLFANYYPVQL
                     FEKGKGPELYPFSVGLVLVPFD"
     gene            complement(1208..>1522)
                     /locus_tag="PK049_09540"
     CDS             complement(1208..>1522)
                     /locus_tag="PK049_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61839.1"
                     /translation="DEEIEPIATIEPQVTQTAQQEAQPNISINKSNGLTTQGSISAKQ
                     VQPQTKELTLGDIALKGVQKLAQSVGINVDVKQTEGNQAKKIVVESKLLAVSATILPK
                     EE"
     assembly_gap    1523..1622
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1623..1951)
                     /locus_tag="PK049_09545"
     CDS             complement(<1623..1951)
                     /locus_tag="PK049_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61840.1"
                     /translation="MTINRNNYELFIIDYIDGKLSPELTDELMVFLNQNPDIALEVDG
                     LAEIKLNPTPVKAPMAKESLKRSTSLTESGITEADYLCIAELENDLTAHESLQLNELK
                     KNHPDIA"
     gene            complement(1956..2441)
                     /locus_tag="PK049_09550"
     CDS             complement(1956..2441)
                     /locus_tag="PK049_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOK61841.1"
                     /translation="MTAEEFNRCVDLYSDSIYRFALKMLKNTDLAMDNVQDCFERLWV
                     KHREVDFAKAKSYLFTSAYHAAIDSIRKEKRKLEYAGEAFFETSTSNGYSDAKEIVDK
                     AVEKLPEIQKSVLLLRDYEGYSYEEIGEITGLNESQVKVYIYRARLFLKEQLVSIDNL
                     I"
     gene            2587..3888
                     /gene="purD"
                     /locus_tag="PK049_09555"
     CDS             2587..3888
                     /gene="purD"
                     /locus_tag="PK049_09555"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOK61842.1"
                     /translation="MDKKLNVLLLGSGGREHALAWKISQSPLLEKLFIAPGNPGTGYH
                     GQNVAINPLNFDEVKGFCVQSAIDMVIVGPEEPLVQGIHDSFLSDSNVKHIPVIGPQK
                     LGAMLEGSKDFAKEFMFRHGIPTAQYKTFTAEKFNQAKDFLKTLNPPYVLKADGLAAG
                     KGVVIVPSYDEACANLQEFFAGKFGAASQKVVIEEFLSGIELSVFVLTDGHSYIILPE
                     AKDYKRIGDGDTGPNTGGMGAVSPVPFANQDFMKKVEERIVKPTINGLRNDAIPYKGF
                     IFIGLMNVDGDPYVIEYNVRMGDPETEVVMPRIKSDLLAALLACSQNRLSEASIEVDS
                     QTATTVIVVSGGYPNSYEKGKEIATPASIPYNAILFHAGTSISNGKLVTAGGRVFACS
                     ALGNGIDQALEKSYTLAKAIKFDKLYYRNDIGNDLKKLQNQ"
     gene            3890..4885
                     /locus_tag="PK049_09560"
     CDS             3890..4885
                     /locus_tag="PK049_09560"
                     /inference="COORDINATES: protein motif:HMM:NF040411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6427 family protein"
                     /protein_id="HOK61843.1"
                     /translation="MLLKSAQKSRFGALVFILLLLFATWLTTLMGKSPTYSFLFDSFP
                     MPFYQAVANLLSHYPIGYKLTAVILFFISGIYLLVLNNKFIILKNRSYLPILFYALCS
                     AAFLPIQRLNPALVSVFLVLLSIDHLFAIYQKNAPLDNLFRAGLAIGLATLLYAPAIT
                     IAVWALLTLLVLRPFNVREWFVLLFAFTVPWLIYMLILFIINDDILKPYHAFVTSIFT
                     ESEPAIDDKLFWIFGGFLAIPFGVSLIGTYQKLAFQKIIIRRYYSIFLYLFLIGVVSF
                     FLVPGVSYEIFYFTAIPSAFIMSSYFADAKQNFWNELLLWLPIIGAIVVQVVMLI"
     gene            4959..5492
                     /locus_tag="PK049_09565"
     CDS             4959..5492
                     /locus_tag="PK049_09565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOK61844.1"
                     /translation="MKYAIVDIETSGGSWQRDRITEIAIYIHNGNSITNEFKTLVNPE
                     TIIPYYITQLTGITNGMVANAPKFYEIAREVVLLTQDCVFVAHNAPFDYRFIQAEFKR
                     LGYDYHREVLCTVKLSRRVFPGYGSYSLGNICNTLGIEIENRHRAAGDALATTKLFEK
                     ILFETNGDFTPFVKAKR"
     gene            5610..5888
                     /locus_tag="PK049_09570"
     CDS             5610..5888
                     /locus_tag="PK049_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61845.1"
                     /translation="MNIRKLYSHPYSAILAALLTASVFIFMFIYLAVTQRKHIYESSK
                     EVSKEVLRQTAGETQLYFTAAYVVANSIQQRAIILKQKMEPATILRIF"
     gene            5873..9442
                     /locus_tag="PK049_09575"
     CDS             5873..9442
                     /locus_tag="PK049_09575"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOK61846.1"
                     /translation="MKNLLKDVLTSNPNILGAWVLWESNAYDQNDSRFNHSNEFPCNG
                     RFGITYFRDNDTLYSEIVPCNDYKSFFYTDAKQKRSAVLSEPYLFRYTNYSNLYFGCT
                     ISVPIMSDTAFLGVVGVDINLSFLQNAISSVKLFDTGFLYLVSPKGLIVSYPDSAKIR
                     KNFYLEESRADSAYTAKAFMGNEVSAETYSNYLKERVFRFYYPINLYLSDKPWMVISE
                     IPIEKASSRSRQVVWVATFVMVIGILLIIYLVLNIFERRKYQQRIEDALCEAEKQKAN
                     AVESFQNYLEIFNSTSEAIFIYDAETGVIIDVNDQMLKMYGLHSKEEVIGKHVDVFSS
                     GIEPYDANGAQERIKQAIKAGSAVFEWQSKRSDGSIFWSEVSLKFTFISGKKTVLAVV
                     RDVSDRKIAQQALEYRERKFRELADLLPLVVWEVDLNARFTYANNIGFKLFGYTQEDL
                     DKGITIIDLIAPEDRQRVAQNIKKLFIESKTSDEEYLALRKDGSIFPANIFSSVVYEN
                     NIPKGLRGILIDVTEKKKAEQEIKEREQLYRTVVESLNEALMMVDNNDRILFVNTRFT
                     EMFGYKPEEVIGKVGNEFLIPEQRRHEIVEANNQRKQGIKNVYEMQFKAKDGRMVDCM
                     VSGAPVYNAQGAVTGSIGAIVDITELRKAEKAYHETMMLFETLSHMSPVGIFRTDAQG
                     KTTYVNPKWCELSGLTPEQAKGDDWIKAVHPEDRQKILDGWYERVNLEESSSTEYRFL
                     KPDGSVVWVLGNALPEKNADGINGYIGTITDITQIKQAQEKLERSERRFRELTELLPQ
                     SIWEIDLNGMVTFVNKYGLESMGYTYEDFKKGVNIFETIVPEFREETIRLFKRRLQGE
                     TAPGLEYPAQRRNGERFPALVYSSPIVDNGIVVGLRGISIDITEIKKTQQELETYRNH
                     LELLVKERTEELEAVNEELTSANEELYNQREKLIETLQELKNAQQTLIQSEKMASLGL
                     LAAGVAHEINNPLNFIQGGIIAIEGFINDKFKAQKSELQPLVDAIKEGVKRSAQIVRS
                     LNQYSRADYTKHSNVDLHRVIDNCLILLSNQYRDRIEIIKHYNEDSTSIYCNEGQIHQ
                     SFMNILINAIQAIDGTGKITITTKSTDKTCRVIFEDTGCGIDHENLPKIFDPFFTTKE
                     PGVGTGLGLSITYNIIKEHKGNINIHSERGKGTKVTVVLPKSS"
     assembly_gap    9594..9693
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9694..10535)
                     /locus_tag="PK049_09580"
     CDS             complement(<9694..10535)
                     /locus_tag="PK049_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HOK61847.1"
                     /translation="MISFWKRDIGLHPTAEWEPYCWVNVENPTNEEKRYLLDELGVPD
                     AFYNDIEDVDERPRIEYENGWFFILMRLPYKNTDLKIPYTTVPLGIIFKDEVFVSISF
                     YRCEVIPDFIQFSVRKGILIKDHFDQVLRIMLSSSVWFLKYLKQINNDIKEAENQLER
                     SIRNEELQDLLRIEKSLVFFTTSLKGNDILLHRIKNLRSYRDTYNPELLEDVEIELRQ
                     AQETTSVYSDILSGMMDAYASVISNNLNIVMKRLTSISIVLMIPTLIASFYGMNVPNN
                     LESHP"
     gene            complement(10827..11045)
                     /locus_tag="PK049_09585"
     CDS             complement(10827..11045)
                     /locus_tag="PK049_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61848.1"
                     /translation="MGAPWVELPRDTIRHAYGVTHVVSLTKPTATFHPRPIVGSGFYA
                     TAYAIPIGRGILKVKSCRLKEKLRMGSA"
     gene            11743..>12134
                     /locus_tag="PK049_09590"
     CDS             11743..>12134
                     /locus_tag="PK049_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HOK61849.1"
                     /translation="MIDNLFWIVPIASVIALAFAYHFFRQMMREDEGTDLMKKIAQHV
                     RTGAMSYLKQQYKVVFIVFIVLMLLFVYLAFGLGVQNKWTPFAFLTGGFFSGLAGFFG
                     MKTATYASARTANAAKNSLNHGLRVAFRS"
BASE COUNT         3419 a         2554 c         2503 g         3458 t
ORIGIN      
        1 accatcaact acaaaaggcg ggtaaaaccc gccttttgta acctaaccac taaccgtaaa
       61 ctaatcaaaa ggaaccagaa caagacctac cgagaaagga tatagctcag ggcctttacc
      121 cttttcaaat agctgaaccg ggtagtagtt ggcaaaaagc cttatgccct tgtaacccat
      181 gcgggcggtt aacccatacc gtagggtagc aatgttaaaa tcgccgcggt ttttatcctt
      241 atggcgtgag ccatcgtcgt aaaccacctt agtgtgggag ccaagcctta caccacctat
      301 tacaccggcg gagatgtaaa ttctgtcctt acgcttctcg cccatgggta tctgaaactc
      361 gaggagtagc ggaagagtta ggtgcgacat ggtaatcttt gtcttttcca ccttatagcc
      421 ggcctgaata taggagctat ccacaacggt tacaccattt tcaaccttaa gggttatggg
      481 attggagaaa tggtaatcgt taaactcaag acccaaaccg gtaacaagac ctgccttatc
      541 ggttccaaat ccaatgctgt actgtgtaaa gttgatgttg gtgttccatg agcgtgcctg
      601 ctttaaatcc atccaggcta tatcgccggt ttgcgtcatg gactggttct tgtccatgaa
      661 tccgtttata cccagctcaa aacccgacca atgtcccttg aactttttca cctccttgta
      721 ctcaataatt tcgtcggact ttttaccctt ttcagggatt tcaatcactg ttttgtcctt
      781 ggaatcaaca atcaccatct ttttcttacc gagtcttact accaccgtat cgcccttggc
      841 ggccttaatg gtatcgccca tggtgttaac ccaggccatg ttgtccgaat caaccgtaac
      901 agcattgacc gaggtaacgg tgtcgatacg ttcctcaata attgaatcgc catcaacaac
      961 ctttacaatt tttcgaacaa ttaccatggg ttgcttatcg cttgaagttt gtgcgctttg
     1021 ttgtttgagt gtgtcgggat tttcgagatt acgaagtatg tcctccttgt tctgtgcgct
     1081 tagttgggta ataatggaaa acatcaatcc cacaagtaaa aactttttca ttgttttatc
     1141 agatttagtt taacattcgg ttgttttacc atacgacgcc cgaatgcaaa aaatgttaca
     1201 ggggtagtta ttcttctttt ggcaaaatag ttgccgaaac ggccagcagc ttcgattcaa
     1261 caacaatttt ttttgcctga ttgccttcgg tttgctttac atcaacattt attccaaccg
     1321 attgggcaag cttttgtacg cctttaaggg cgatatcgcc aagggtgagc tcctttgttt
     1381 ggggttgaac ctgtttggcc gaaatagaac cttgagtagt taagccgttg cttttattta
     1441 tgctgatatt gggttgtgcc tcctgctgag ctgtttgggt aacctgaggc tcaatggtgg
     1501 ctattggttc aatttcttcg tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nncttgcgat atcggggtga ttttttttaa gctcgtttag ctgcaaggat tcatgggccg
     1681 ttaaatcatt ctccagctcg gcaatgcaaa ggtaatcggc ctcggtaatg ccgctttcgg
     1741 ttaaactggt cgatcgcttt aggctttcct tagccattgg tgctttaacc ggagttgggt
     1801 taagcttaat ttcagctagc ccatcaacct caagtgcaat atcagggttt tgattcagga
     1861 aaaccataag ctcatcggta agctcagggc taagcttacc atcgatgtaa tcgatgataa
     1921 aaagttcgta gttatttcgg tttatagtca tagccctaaa tcaagttatc gatacttacc
     1981 agctgctcct tcaggaacaa ccttgcccta tagatgtaaa ccttaacctg cgactcgtta
     2041 agccctgtaa tttcgccaat ctcctcgtac gagtacccct cgtaatcacg aagcaggagc
     2101 accgatttct gaatctcggg cagcttctca acggctttat caactatctc cttggcatcg
     2161 gaataaccgt tagaggttga tgtttcaaaa aatgcttcac cagcatactc aagctttcgt
     2221 ttttccttcc ttatgctgtc gatggcagca tggtaggccg aggtgaacag gtacgatttt
     2281 gcctttgcaa agtccacctc acggtgtttt acccataacc gctcaaagca atcctgaacg
     2341 ttatccattg ccaaatcggt attctttagc atctttagcg caaagcggta aatgctatcg
     2401 gagtataagt caacgcatct gttaaactct tcagcggtca tgaaccgtta cttggttatc
     2461 aactttcaaa tgtatgacga ttaagtttca tttaagttac aacgttgagt aaaaaattgt
     2521 tttgcgccaa tcactttcca ttttaaaacg ataactacct ttgtactcaa acacctgcct
     2581 tgaacgatgg ataagaaact aaatgtactt ttacttggct ctggcggccg tgagcacgcc
     2641 ttggcatgga aaatttcgca aagtcccctt ttggaaaaac tctttatagc tccgggaaac
     2701 cccggtactg gctaccatgg ccagaacgta gctattaacc ctttaaattt cgatgaggtt
     2761 aagggctttt gtgttcagag cgcaattgat atggtgattg taggcccgga agaacctctt
     2821 gtacagggca ttcacgattc cttcctgtcc gatagtaacg ttaagcatat accggtaatc
     2881 ggccctcaaa aattaggtgc tatgcttgag ggtagcaagg attttgcaaa ggagttcatg
     2941 ttccgacatg gaatccccac cgctcagtat aaaaccttta cagcggaaaa gttcaaccag
     3001 gccaaagatt ttcttaaaac cctaaatccc ccatacgttc tcaaggccga tggacttgca
     3061 gccggaaagg gagtggttat tgtgccatcg tacgacgagg catgcgcaaa cctccaggag
     3121 ttttttgcgg gtaaatttgg agctgcaagc caaaaggttg tgattgagga gttcctgagc
     3181 ggaatagagc tatcggtctt tgtgctaacc gatggccact cgtacataat cctccctgag
     3241 gcaaaggact acaagcgtat aggcgacggc gataccgggc caaataccgg aggtatgggg
     3301 gctgtgtcgc ctgtaccatt cgccaatcag gattttatga aaaaggttga ggagcgtatt
     3361 gtaaagccta ccataaatgg actcaggaac gatgctatcc cttacaaagg ctttattttt
     3421 attggcctaa tgaacgtcga tggcgatccc tacgtaattg agtacaatgt tcggatgggc
     3481 gatccggaaa ccgaggtggt aatgcctcgc attaaatccg acctgcttgc agccttgctg
     3541 gcttgctcac agaatcggtt atcggaagca tccattgagg ttgattccca aaccgcaaca
     3601 acggttattg ttgtatcggg aggctaccca aattcatacg agaaagggaa ggaaatagct
     3661 acacctgctt ctattcccta taatgctatt ctattccacg ccggaacctc cataagcaat
     3721 ggtaaactgg ttactgctgg cggacgagtt tttgcctgtt cggcgctggg caacggcatc
     3781 gaccaagccc ttgaaaaatc gtacaccctt gctaaagcca tcaaattcga taagctgtac
     3841 taccgaaacg acattggcaa cgatttaaaa aaactgcaaa accaatagta tgcttctcaa
     3901 aagtgcccag aaaagtagat ttggagcatt agtgttcatt ttgcttctcc tgtttgcaac
     3961 ttggcttact acccttatgg gtaaatcgcc aacctactca ttccttttcg atagttttcc
     4021 aatgccgttc taccaagctg tagcaaattt gcttagccat taccctattg ggtacaagct
     4081 aacagctgta atccttttct ttatatcagg aatttacctg ctggtgttaa acaataagtt
     4141 tatcatatta aagaatcggt cgtacctgcc aattctgttt tatgcactat gctctgcggc
     4201 ctttttacct attcaacggt taaacccagc gctggtatca gtattcctgg tgctgctcag
     4261 catcgatcat ttatttgcca tttaccagaa aaacgccccc ctcgacaacc tttttagggc
     4321 agggttagca ataggtttgg ctaccttgct ttatgctcct gcaataacta ttgcagtttg
     4381 ggctctactt actttgctag tactgcgccc cttcaatgtt agggaatggt ttgttttgct
     4441 attcgccttt accgttccct ggcttattta catgcttata cttttcatta ttaacgatga
     4501 tattcttaaa ccttaccatg cttttgtaac aagtattttc accgaatcgg aacctgctat
     4561 cgacgataag ctattttgga tttttggagg ttttctggct attcccttcg gggtatcgct
     4621 tataggtacc tatcaaaaat tggcttttca aaaaattatc attcgccgct actactccat
     4681 tttcctttac ctttttctga ttggggttgt ttcgttcttt ttagttccag gtgtatcgta
     4741 tgagatattc tactttactg ctataccctc agcattcatc atgtcgtcgt attttgctga
     4801 tgctaaacag aacttttgga acgaactttt gctgtggtta ccaattattg gtgcgatagt
     4861 ggttcaggtt gtaatgctga tttgaaaaaa aatttgttag tattggggta tatcgagttc
     4921 atatgccact ggtagcaata cccttaactg ttatgtaaat gaaatatgcc atagttgata
     4981 ttgaaacatc gggaggtagc tggcagcgcg ataggataac tgaaattgcc atttacattc
     5041 acaatgggaa tagtataacc aacgaattta aaacactggt taaccctgaa accataattc
     5101 cctactacat tacacagcta acgggtatca caaatggaat ggtggccaat gcgccaaagt
     5161 tctacgaaat tgctcgggag gtggtactgc tcacccagga ttgcgtattt gtagcccaca
     5221 atgcaccatt cgattaccgg tttattcagg ctgagtttaa acgtttgggc tatgattatc
     5281 accgcgaagt gctctgtacc gttaagctta gccgacgggt tttcccggga tatggctcat
     5341 acagcctggg caacatttgc aacactttag gaattgaaat tgaaaatcgc catagggctg
     5401 ctggcgatgc actggctaca acaaaactct ttgagaaaat tctttttgaa actaacggtg
     5461 attttacccc ttttgttaaa gccaaaaggt aacattttaa taccctggta ttagctaaaa
     5521 cgcttttggt caaaattatc ggctcaatca aatttttttc catataaaat actctatctt
     5581 tacgtatagg aaactgtaca tgcgggtaaa tgaatattcg taaactatac tcacatccct
     5641 attcagcaat acttgcagcc ttgcttacgg ccagcgtgtt tattttcatg tttatctatc
     5701 tggcagtaac tcagcgtaaa cacatttatg aatcgtccaa ggaggtctca aaggaggtgc
     5761 ttcgtcaaac tgctggcgaa acccagctct atttcaccgc agcctatgta gttgcaaatt
     5821 caattcagca acgagcaata atactgaagc aaaaaatgga acccgcaacg atattaagaa
     5881 tcttttaaag gacgttctta ccagcaaccc taatatttta ggggcttggg ttctatggga
     5941 atccaatgct tacgaccaga atgattcccg gtttaatcat agcaatgaat tcccttgcaa
     6001 tggacgtttc gggataacat actttcgcga taatgataca ttatactctg agattgttcc
     6061 atgcaacgat tacaaatcgt tcttttatac cgatgcaaaa caaaagcgca gtgcggttct
     6121 ctctgaacca tacctgttca ggtacaccaa ctatagcaat ctgtattttg gatgtaccat
     6181 ttcagttccc ataatgagcg atacagcctt cctgggtgta gtgggggttg acataaatct
     6241 tagctttcta cagaatgcaa tatcttcagt aaaacttttc gacaccggat ttttatactt
     6301 ggtttcccct aaagggctaa ttgttagcta tcctgattct gctaaaattc ggaaaaattt
     6361 ttacctcgaa gaaagtagag ccgatagtgc ctataccgcc aaggccttta tgggaaatga
     6421 agtttctgct gaaacctatt caaattacct aaaagaaaga gtatttcggt tttactatcc
     6481 aattaaccta tacctgagcg ataaaccctg gatggtaatt agcgaaatac caattgagaa
     6541 agcatcgtca cgttcaaggc aagtggtatg ggtagcaact tttgtaatgg ttattggtat
     6601 actacttatt atttacctcg ttctcaacat ctttgagcgc cgtaaatacc agcaacgtat
     6661 cgaggatgca ctctgtgagg cagagaaaca gaaagccaat gcggttgaaa gtttccaaaa
     6721 ctaccttgaa atcttcaact ctaccagcga agctattttt atttacgatg ccgaaacagg
     6781 agtaattatc gatgtaaacg accagatgct taaaatgtat ggattacata gtaaggaaga
     6841 ggtaataggt aagcatgtcg atgttttctc ctcaggaatt gaaccatacg atgctaatgg
     6901 agcccaggaa cgaataaaac aagcaataaa ggctggtagt gctgtttttg aatggcaaag
     6961 caaacgatcc gatggctcaa ttttctggtc tgaggtatcg cttaagttca cttttattag
     7021 cggtaaaaaa acggtgcttg ctgtagtccg cgatgttagc gatcggaaaa ttgcccagca
     7081 agcactggag taccgcgagc gtaagttccg tgaactagcc gaccttttac ccctggttgt
     7141 ttgggaggtt gacctaaatg cgcgatttac ctatgccaac aacattggct tcaaactttt
     7201 cggctatacc caggaggatc tggacaaggg catcaccatt atcgacctca ttgcccctga
     7261 agacaggcag agggttgccc aaaatattaa aaaactcttt attgaaagta aaacatctga
     7321 cgaggagtac cttgccctca gaaaggatgg ttcaattttc ccagccaaca tattctcaag
     7381 cgttgtttac gagaacaata tccctaaagg gcttagaggg attctaattg atgtaaccga
     7441 aaagaaaaag gctgaacaag aaatcaagga aagggaacaa ctctatagaa ctgtagttga
     7501 aagcctgaat gaggcgctta tgatggtcga taacaacgac cgtatactat ttgtgaacac
     7561 ccgatttaca gagatgtttg gatataagcc ggaagaggta attgggaaag taggaaatga
     7621 gtttttaatt cctgagcaaa ggcgacatga aatagttgag gcaaataatc aacggaaaca
     7681 ggggataaag aatgtttacg aaatgcagtt caaggccaaa gatggccgaa tggtcgattg
     7741 catggttagc ggtgcaccag tttacaatgc tcagggtgct gtaactggtt ctattggagc
     7801 aatagtcgat atcaccgaac tccgaaaagc cgaaaaagca taccatgaaa ccatgatgct
     7861 tttcgagacc ctctcccaca tgtcgcccgt aggcatcttc cgaaccgacg ctcagggtaa
     7921 aaccacctat gttaatccaa agtggtgcga actatccgga ttaacacccg aacaagcaaa
     7981 aggcgatgac tggattaagg ctgttcaccc ggaagatagg caaaagatac tcgatggttg
     8041 gtacgaaagg gttaacttag aggaaagctc atcaaccgaa taccgattcc taaaacccga
     8101 tggttcggtg gtttgggttc tgggcaatgc tttgcccgaa aaaaatgccg atggaatcaa
     8161 tggttatata ggaaccatca ccgatatcac ccaaataaaa caagcccagg aaaagcttga
     8221 acggagcgag cggcgattcc gtgaacttac cgaactccta ccccaatcca tttgggaaat
     8281 tgacctaaac ggaatggtaa cctttgtgaa caagtatgga ctggaaagta tgggttacac
     8341 ctatgaggac tttaaaaaag gggtgaatat ttttgaaacc attgttcctg aatttaggga
     8401 agaaactatc aggcttttta aacgacggct acagggggag actgcacctg ggcttgagta
     8461 cccagcacaa cgcaggaacg gcgaacgatt ccctgctctt gtgtactcat cccctatagt
     8521 tgataacgga atagttgttg ggttaagggg gatttccatc gatattacag aaataaagaa
     8581 aacccaacag gaacttgaaa cctatagaaa ccacttggaa ctacttgtaa aagaacgtac
     8641 tgaggaactt gaggcagtaa acgaggagct aacctcagcc aacgaggaac tttacaatca
     8701 gcgcgaaaag ctaatagaaa ccttacaaga actaaagaac gctcagcaaa ccctaatcca
     8761 atcggagaaa atggcatcgc ttggtttgct tgcggctggg gttgctcacg aaataaataa
     8821 ccccctgaat ttcatacagg gaggcattat tgccattgag ggatttatca acgataagtt
     8881 taaagcacaa aaaagcgagc tacagccatt agttgatgct atcaaggaag gagttaaacg
     8941 ctcagcccag attgtaagaa gccttaacca gtatagccgt gccgactata caaaacactc
     9001 aaatgttgat ttacacaggg tgattgacaa ctgtctgata ctactttcca atcagtaccg
     9061 cgatagaatt gagatcataa agcattacaa cgaggattca acaagtattt actgtaacga
     9121 gggccaaata caccaatcgt tcatgaatat tctcatcaac gccattcaag ctatcgatgg
     9181 tacaggtaaa ataaccataa caacaaaatc aacggacaaa acgtgtaggg taatttttga
     9241 ggataccgga tgcggaattg accatgaaaa tctgccaaag attttcgatc cgttctttac
     9301 caccaaggag cccggagtgg gaacagggct ggggttatcc atcacttaca acattataaa
     9361 ggaacacaaa ggaaatatta acatacactc cgaaaggggc aaaggaacaa aagttacagt
     9421 agttttacct aaatcaagct agccatggat gcaacaggaa atatcagcgt gctccacatc
     9481 gacgatgagc caacaaacgt tctgcttttt gaggtaaact tccggtggta ctaccgggtt
     9541 gtaagcgcca attccggtgt cgatgggtta acaaaactta cccaataccc aaannnnnnn
     9601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnggatgac tttccaggtt attgggaaca
     9721 ttcatcccat aaaagctagc aattagtgtt ggaatcataa gcacaatgga gatagaggtt
     9781 aaacgcttca tcacaatgtt taggttattg gatataaccg aagcataggc atccatcata
     9841 ccgcttagaa tgtcggagta aacacttgta gtttcctgtg cctggcgcaa ttcaatctct
     9901 acgtcctcca acagttctgg attataggta tcgcggtaac tacgcaggtt ttttatcctg
     9961 tgcagcagta tatcgttgcc ctttagcgat gtggtaaaga acaccaaact cttctcaatg
    10021 cgaagcagat cctgtagctc ctcgttacgg atggaacgtt caagctggtt ttcggcctcc
    10081 ttaatatcgt tgtttatctg ttttaggtat ttcaggaacc atacacttga cgatagcatt
    10141 atcctgagta cctgatcgaa atgatccttt attaaaattc ccttacgaac cgagaattga
    10201 atgaaatcag ggatcacctc gcaacggtag aaactgattg acacaaatac ctcatcctta
    10261 aaaataatac caagagggac tgtggtgtaa ggaattttca gatcggtatt cttataaggt
    10321 aaacgcatca gaataaaaaa ccacccgttc tcatactcaa tacgggggcg ctcgtcaacg
    10381 tcctcaatat cgttgtagaa agcatcgggc accccgagct catcgagcaa atagcgtttt
    10441 tcctcgttag ttggattttc aacgtttacc cagcagtagg gctcccattc ggcggttgga
    10501 tgtaaaccaa tgtccctttt ccagaatgaa atcatgtact atccctccaa aattttatag
    10561 aatagaggta tagtacatta cctctattcc gggttaacta tcagggtgat aatcgtccat
    10621 attgaaattt gactttttcc gaccgtgcaa agttaatttt ttttgtttta atactaccat
    10681 cttatatgaa tgtaatgctt tttttgtgaa tattctttag ctgacttata aattagaacc
    10741 aatgcttttt agagatattt gtagtggtga atcccaattt actaggcctt ggaacgataa
    10801 aacctttggc attagaactc cccctgtcat gctgagccca ttcgaagctt ctctttgagc
    10861 ctacaacttt taactttcaa aattcctcga ccaataggaa tggcataagc agtggcataa
    10921 aagcctgacc cgacaatggg tcgggggtgg aacgttgcgg tgggcttagt aagcgatacc
    10981 acatgagtca cgccgtaggc gtggcgaata gtatcgcgtg gcagttccac ccacggggca
    11041 cccatcgggt cgggcttttc agccttgatt ttctttggtt ctttcttgta tcaagacaag
    11101 aaagaacgtg taaatcgctc tttgcattgc ctttctgttg cgatgcaact ttttgtcctt
    11161 gctctttgga aaatgtatta cgtgcatcgc tatatatatg ggagtagtta tggaattaca
    11221 aatgtttagc ccctacaggg caggatgcgt tgttcgttgt tcgtgaatcg ttgttcgtga
    11281 aatgagatgg aacgcagatg acacagatta ggcagattta cactgtgata tcaatttagt
    11341 tgatggttga tagttgatag ttgatggttt aagaaaccac cttaaatcat gaatctgaaa
    11401 tctgaaattt taaattttta tcctttatcc tttaaccttt atcctttatc cttacttccc
    11461 ctgtacactg aacccttcta ctaattcagt ttgacaaatg gagatttact tttggtaact
    11521 aaacgaaatt gagatcaaaa tttaaatcct aataaaccta cctctagtat ctttctacga
    11581 acatccttta ataaaatttg ttaatgaaaa aacttttaag tcaacctctt ttttttgttc
    11641 gaatatactc accttaaatt acacttcgta tattagctaa atgtttgtat gaaaattatc
    11701 tatataaata tatcaataac aacctaaatt attaaaccta tgattattga caatttgttc
    11761 tggattgtac cgattgcgtc ggtgattgcc ctggcgtttg cctaccactt ttttaggcag
    11821 atgatgaggg aagatgaggg tactgacctg atgaaaaaga ttgcacagca cgtgcgtacg
    11881 ggtgccatgt cgtacctaaa gcagcagtac aaggtggtgt ttattgtatt tatagtgctg
    11941 atgctgcttt ttgtttactt ggcatttgga ctgggagtgc aaaacaaatg gacaccgttt
    12001 gcattcctaa ccggtggttt cttttccggt ttagccggat tttttgggat gaaaaccgcc
    12061 acatacgcct cggctcgaac agctaacgct gctaagaact ccctaaacca tggccttagg
    12121 gtagcattca ggtc
//
LOCUS       DAOSAL010000075        11797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_3657_length_11797_cov_47.619826, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000075 DAOSAL010000000
VERSION     DAOSAL010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 11797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11797
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3657_length_11797_cov_47.619826"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /gene="argS"
                     /locus_tag="PK049_09595"
     CDS             complement(<1..1045)
                     /gene="argS"
                     /locus_tag="PK049_09595"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007900622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOK61850.1"
                     /translation="MTAEKFIQVLVKSAAEKLYNQPIADSLIQIQKTRKEFEGDYTMV
                     AFPLLKLSRKSPEETCKEIGEQLVALDKHIESFNVIKGFLNITLSKSYWANVLNTIVQ
                     TNDFGFCEAKSDKPIVIEFSSPNTNKPLHLGHIRNNLLGMSISRIMEKSGKRVVKVNL
                     VNDRGIHICKSMLAWQKFGNGETPELTGVKGDHLVGDYYVRFDKEYKAQVEELKAKGM
                     PEDEAKLKAPIIQEAQEMLRRWEAKDPEVIHLWKTMNGWVYEGFDKTYADLGITFDKV
                     YHESETYLLGKSVVMDGLNRGIFFRKDDGSVWVDLTNDGLDQKLLLRGDGTSVYITQD
                     IGTAIQRYNEFNFD"
     gene            complement(1069..4350)
                     /gene="secA"
                     /locus_tag="PK049_09600"
     CDS             complement(1069..4350)
                     /gene="secA"
                     /locus_tag="PK049_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOK61851.1"
                     /translation="MGLSNFLTKFFGTKSDRDLRELMPIVNKIREAYPRYTTLTNDQL
                     REETDRLRKVVRDYIAEDEAKVEQLKAQMESDEIDYEEKERVYKEVERLTKQIDDKIE
                     EVLNQVLPDAFSIVKETARRFKENEIIEVTATQFDRDLAARKDFVTIQGDKAIYKNRW
                     MAGGNEIVWDMVHYDVQLIGGVVLHQGKIAEMATGEGKTLVATLPVFLNALAGKGVHV
                     VTVNDYLARRDSEWMGPIYEFHGLSVDCIDKHQPNSEARRKAYNCDITYGTNNEFGFD
                     YLRDNMAIAPDDLVQRKHHYAIVDEVDSVLIDDARTPLIISGPVPKGEDQMFEEYKPK
                     VEKLVNAQRSLITQLLTDARKLISEGNTEEGGKLLLRAHKGWPKYKPLIKFLSEQGNK
                     ALLLKTENFYMQDNNRNMHLITDDLYFVIDEKQNSVELTDKGIDLITSSAEDPKFFVL
                     PDIGSEIAEIEKMSITQGEKLTKKDELLREYAVKSERVHTVHQLLKAYTMFEKDVEYV
                     VMDNKVKIVDEQTGRILEGRRYSDGLHQAIEAKERVKVEAATQTFATITLQNYFRMYH
                     KLAGMTGTAETEAGEFWSIYKLDVVVIPTNKPVVRKDMEDLVYKTKREKYNAVIDEIV
                     RLRDANRPVLVGTTSVEVSELLSRMLKLKGIQHNVLNAKQHQREAEIVAHAGIPKTVT
                     IATNMAGRGTDIKLTPESRAAGGLAIIGTERHESRRVDRQLRGRSGRQGDPGTSQFYV
                     SLEDDLMRLFGSDRIARVMDAMGHKEGDVIQHSMVSKSIERAQKKVEENNFGIRKRLL
                     EYDDVMNAQREVIYKRRRHALFGERLDVDISNMFMDVCEAIVNEFHGNYEYDDFKLEI
                     IKQFSLDVPITEDEYLELKPSEIAHRLYKVALESYDRKAHEIARQAYPVIKEVYEKQS
                     AIYENIVVPISDGQKVYQTVTNLKKAYETGGKELIRSFTKNIILFTIDEYWKEHLREM
                     DELKQSVQNAVYEQKDPLLIYKLEAFDLFRTMIDKLNRDIVAVLVKAFIPLRDPNQVQ
                     EARQSRRTDMSQYQTSRTDNLQPGANTQEQSRQPVKVEVKVGRNDPCPCGSGKKYKNC
                     HGKNL"
     gene            complement(4599..5111)
                     /locus_tag="PK049_09605"
     CDS             complement(4599..5111)
                     /locus_tag="PK049_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HOK61852.1"
                     /translation="MNKRLEQAINEQINAEIWSAYLYLSMSAHFGAQGFNGFANWFKV
                     QWQEELTHAMKFFDYLIERGSKPELKPIKEVPTKWDSPLHAFEETLKHEKEVTRLINN
                     LMDIAIEEKDHAAKGMLQWFVDEQVEEEANAQELIDTLKLINGNGNGLFMLDRELKQR
                     AFVGDTKKAE"
     gene            complement(5168..6880)
                     /locus_tag="PK049_09610"
     CDS             complement(5168..6880)
                     /locus_tag="PK049_09610"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOK61853.1"
                     /translation="MENFIVSARKYRPATFKSVVGQASITTTLKNAIQRKQLAQAYLF
                     CGPRGVGKTTCARIFAKTINCQNLSPELEACNECESCKSFNTNRSYNIHELDAASNNS
                     VEDIRKLIEQVRIPPQIGKYSVYIIDEVHMLSQSAFNAFLKTLEEPPAHAIFIMATTE
                     KHKILPTILSRCQIFDFNRIRVEDTVNYLEYIAKKEGVEYEVEALNIIAQKADGAMRD
                     ALSIFDQVVSFSENHITYQKVIENLNVLDYQYYFRLTDNFLKGDYADSLQIFDELLSK
                     GFDAQQFITGLSMHLRNVLVCHDPKTIPLLEVGAAIAQKYKEMAATCPPGFLIEALQV
                     TNQFDVAFRQSPNQRLHVELMLIKLCGLNQKKKSDDSQVEDSDPVEYSQPISSTVKPT
                     STPKAIPTTPKTPIRTTSIKGALNGAPNNAEIKNNDEPKVEEIQTENQGIASVEIKKI
                     SHEQILQAWTSFAEQIQKDKPAHFSLMQVYKPILKENNKITIQFEGQIQIELFSEIKK
                     DLLTYIKKSFGILNIEIAEEIVEKIEDNGKPRLYTPDDKFRFMAERNPALIRLKQQLN
                     LDLD"
     gene            complement(7490..8632)
                     /locus_tag="PK049_09615"
     CDS             complement(7490..8632)
                     /locus_tag="PK049_09615"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOK61854.1"
                     /translation="MKIPQIAIFLSIVLSVYALVNLFIFYQTRPVFSLASIGTWLKFV
                     FWFIVLSYPLGRTLEAILGGQIPTLLVKVGSIWLAFMLYLTLFFLLFQITSPLINWLV
                     KIDIKSSLETHRMIVGMVYFATLTVILAGYINAVNPKINQLKIESSKPVPGGGLTIAM
                     VSDIHLGTIIGKNELSSLVDKINSQKPDIVLFVGDIFDEDIAPVVNGQMGEQFERINA
                     KYGVFAVTGNHEFFSNHQAIIEYLQKHGVNVLNDTVAEVANINIVGRYDRQSNFALGQ
                     SRKTLSELTKDIDQSKFTIVLDHQPFNLNESAESGADLQLSGHTHHGQMWPLNYITRA
                     VYEVSMGYKKIGNTHIYVSPGYGTWGPRVRLGNRPEIAVIRVKDKG"
     gene            complement(8730..8921)
                     /locus_tag="PK049_09620"
     CDS             complement(8730..8921)
                     /locus_tag="PK049_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004570569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOK61855.1"
                     /translation="MNKGTVKFFNESKGFGFIKDSETGEEYFVHATGLKDQISENDEV
                     TFNLEQGRKGLNAVDVKLT"
     gene            complement(9120..11420)
                     /locus_tag="PK049_09625"
     CDS             complement(9120..11420)
                     /locus_tag="PK049_09625"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HOK61856.1"
                     /translation="MGKYCSTLTITLLFSTCAIFAQPKNYIKWVNPFIGTERMGHTFP
                     GATVPFGAVQLSPDTDTIPYSSNGKYNPDVYKYCAGYQYGDSTIIGFSHTHFSGTGHS
                     DLGDILVMPTVGKLHLNPGTASNTNTGYRSKFSHSNEFAEPNYYRVKLLDYNILAELT
                     TTTRVGLHRYTFPETDSAHIIIDLVHGIYNYPEKNVWTFIRVENDTLITGYRQTSGWA
                     RTRTVYFAITFSKPFKTYGIINGDKLIYKGFWRKFNQNTNFPENAGREIRCHFDFSTA
                     NHEQVLLKVALSPVSTSGAVENLNAEAPGWDFDAIKKEGQEQWNDELGKIEVETLTDS
                     DKVNFYTSMYHAFLSPTIYMDTDRKYRGLDQNIYTATNFDNYTTFSLWDTYRALHPLF
                     NIIQPKRNVDMVKSMLAHYSQSVHRMLPIWSHYANENWCMIGYHAVSVIADAAVKGND
                     GFDLPYALMACVQSAKNNWYDGLGYYREFGFVPEDISGSSVSKTLEYAYDDWCIAQLA
                     KKLDREDIYNEFTSRSNNFQNVFDKSIGFMRPRLSNGSFKTSFNVLSTHGQGFIEGNS
                     WNYSLYVPHNPDALVSMLGGKKQLRAYLDTLFTMHLPDEYFAETEDITRDGLIGNYVH
                     GNEPSHHVPYLYNWTDNPWKTQYWVRTICNLMYQPKPDGLSGNDDCGQMSAWYIFSAL
                     GFYPVAPASPNYAIGSPLVTKATIKLENGKTLTISTQNQAKNNVYVQKVLLNGKPYNS
                     YFLNHNDIVSGGEITFIMGDKPKKLK"
BASE COUNT         3100 a         2484 c         2435 g         3778 t
ORIGIN      
        1 catcaaagtt gaactcattg tatcgttgaa tggctgtgcc aatatcctgt gtgatgtata
       61 ccgacgttcc atcgccacgt aaaagcaatt tctggtccaa tccgtcattt gtaagatcaa
      121 cccacactga accatcatcc ttcctgaaga atattcctcg gtttaaccca tccataacca
      181 ccgattttcc gagtaggtaa gtctcggact cgtggtaaac cttgtcgaat gtaattccta
      241 aatcggcgta agttttatca aatccctcat aaacccaacc attcatggtt ttccataggt
      301 gaataacctc tggatccttg gcttcccagc gtcgaagcat ttcctgtgcc tcctgaataa
      361 ttggggcttt tagttttgcc tcatcctcgg gcatcccctt ggcttttagc tcctcaacct
      421 gggccttgta ctccttatcg aatctaacgt agtaatcgcc caccaggtga tcgcccttta
      481 ctccagttaa ttcaggtgtt tcgccattgc caaatttttg ccaggcaagc atcgatttgc
      541 agatatggat gccacgatcg ttgaccaggt ttactttaac tacccgtttt cctgatttct
      601 ccatgattcg ggaaattgac attccaagaa ggttattcct gatatgtcca aggtgcaagg
      661 gtttatttgt gttgggcgat gagaattcaa taactatcgg tttatccgat ttggcctcac
      721 aaaacccaaa atcgttggtt tgaacaatgg tattcagtac attagcccag tacgattttg
      781 aaagggttat gttcaggaaa cctttgatta cattgaaact ctcaatatgt ttatctaatg
      841 ctacaagttg ttcgccaatt tccttacagg tttcctcggg cgatttacgc gacaacttaa
      901 gcagcggaaa ggcgaccatg gtgtagtcgc cttcaaattc ctttcttgtt ttttgtatct
      961 gtattaaact atcggcaatt ggttggttgt atagtttttc agctgccgat tttaccaata
     1021 cctgaatgaa cttctctgct gtcatttttc cttttggttc agaaataatt ataggttttt
     1081 accgtgacaa ttcttgtact ttttaccgct accgcaaggg catggatcgt tacgtcctac
     1141 cttaacttca acttttactg gttgacggct ctgctcctgg gtgtttgcgc caggctgtaa
     1201 attatcggta cggctggttt ggtactggct catatcggta cgtcggcttt gacgggcttc
     1261 ctgtacctgg ttgggatcgc gaagcggaat gaacgctttc acaagaaccg caacaatatc
     1321 gcggttaagc ttatcaatca ttgtgcggaa aaggtcaaat gcttccagct tatagatgag
     1381 cagtgggtct ttttgctcgt acacggcatt ctgaacgctc tgttttagtt cgtccatttc
     1441 acgcaggtgt tccttccagt actcatcaat agtgaacagt atgatgttct ttgtgaaaga
     1501 acgaattagt tccttaccac ctgtctcgta agcctttttc agatttgtaa cggtttgata
     1561 aaccttttga ccgtcggata ttggtaccac gatattctcg tagatggccg attgtttttc
     1621 gtaaacctct ttgattaccg ggtaggcctg tcgtgcaatt tcatgcgcct ttcgatcgta
     1681 cgattctagg gcaaccttgt aaagcctatg ggctatctct gatggtttaa gctcaaggta
     1741 ctcgtcttcg gttataggga catcgagtga aaattgcttg atgatctcta gcttgaaatc
     1801 atcgtactcg taattaccat ggaactcgtt aactattgcc tcgcaaacat ccatgaacat
     1861 atttgagata tccacatcga gcctttcgcc aaagagtgca tggcggcgac gcttgtaaat
     1921 aacctcgcgc tgagcattca ttacgtcgtc gtactccagc aaacgcttac ggattccgaa
     1981 gttgttctcc tcaacctttt tctgtgcgcg ttcaatggat tttgatacca ttgagtgctg
     2041 aattacatcg ccctccttat gccccatggc atccattacc cttgcgatgc ggtccgagcc
     2101 aaaaaggcgc attaaatcat cctccagtga aacatagaat tgcgatgttc ccggatcgcc
     2161 ctggcgtccc gaacgaccac gcagctggcg gtcaacacgc ctggattcat ggcgctcagt
     2221 accaataata gccaaaccgc ccgcagcacg cgattcaggg gttagcttaa tgtcagtacc
     2281 acgacctgcc atgttggtgg caatggttac cgttttgggt atacctgcat gtgccacaat
     2341 ttctgcctcg cgttggtgtt gcttggcgtt aagcacatta tgctgtattc ctttaagctt
     2401 tagcattcgg ctcagcaatt ccgacacctc aaccgaggta gtaccaacca gtaccggacg
     2461 gtttgcatcg cggagtctaa ctatctcatc aattactgca ttgtacttct cgcgtttggt
     2521 tttgtaaacc aaatcttcca tatccttgcg gacaaccggt ttgtttgtgg ggataactac
     2581 cacatcaagt ttatagatac tccagaactc accagcctca gtttcagcag taccggtcat
     2641 acctgccagc ttatggtaca tacggaagta gttctgaagt gtaattgtgg caaacgtttg
     2701 agtggcagcc tcaaccttaa cgcgctcctt ggcctcaatg gcctggtgaa gaccatcgga
     2761 atacctacgc ccctccagga tacgacctgt ttgctcgtca acaattttta ccttgttatc
     2821 cattacaaca tactccacat ccttctcgaa catggtgtag gcctttagta gctgatgaac
     2881 agtatgtacg cgctccgatt ttacggcata ttccctaagt aactcatcct ttttggttag
     2941 cttttcgccc tgtgttatcg acatcttctc gatttcggcg atttcgcttc ctatgtcggg
     3001 tagaacgaaa aacttcggat cttcggctga actggtgatg aggtcaatcc ccttatcggt
     3061 tagctcaaca gagttttgct tttcgtcaat tacaaagtat aaatcgtcag taataaggtg
     3121 catgttacgg ttgttatcct gcatgtaaaa gttttcagtc ttaagcagga gtgccttatt
     3181 accttgttcg cttaggaatt tgataagggg tttgtatttt ggccaaccct tgtgagcgcg
     3241 gagtagtagt tttcctccct cctcggtatt cccttcggat ataagtttac gagcatcagt
     3301 taaaagttgg gtaataagag aacgctgggc attaacaagt ttctcaacct ttggcttgta
     3361 ctcctcaaac atttgatcct cgccttttgg aacgggacct gagatgatta gcggggttcg
     3421 agcatcgtca attaaaaccg agtcaacctc gtccacaatg gcatagtggt gctttcgttg
     3481 aaccagatca tcgggagcaa ttgccatgtt atcgcgtagg tagtcgaaac cgaactcgtt
     3541 gtttgttcca taggtgatat cgcagttgta ggccttacgg cgagcctcag agttgggttg
     3601 atgcttatcg atacagtcca ctgaaagtcc gtggaactca tatattggcc ccatccactc
     3661 cgagtcgcgg cgagccagat aatcgtttac ggtaaccaca tgtacgcctt tgccggcaag
     3721 ggcattcagg aacaccggta gtgtggcaac cagcgtttta ccttcacctg ttgccatttc
     3781 ggcaattttc ccctgatgca gtacaacgcc accaatgagc tgtacgtcgt agtgaaccat
     3841 atcccacaca atttcattac ctccggccat ccagcggttt ttgtaaatgg ccttatcgcc
     3901 ttgtatggta acaaaatcct ttcgggcggc aaggtcacgg tcaaactgtg tggctgttac
     3961 ctcaatgatt tcgttctcct tgaacctacg tgcggtttcc ttgacaatgg aaaaggcatc
     4021 gggaagtacc tggttaagca cctcctcaat tttatcgtca atctgctttg ttaatcgctc
     4081 aacttccttg taaacccttt ctttttcttc atagtcaatc tcatccgact ccatctgggc
     4141 tttaagctgc tccactttag cctcatcctc agctatataa tcgcgcacta ccttgcgtag
     4201 cctatcagtc tcttccctta gctgatcgtt tgttaaggtg gtatatcggg ggtaagcctc
     4261 cctaattttg tttactatag gcatcagttc acgtaaatcg cgatccgatt tggttccaaa
     4321 aaatttggtt agaaaattac ttaagcccat tttgtaaaaa tttaagattt tttgaagctt
     4381 acaaatttac ttgaattttt tagataacga aaggatacta ccgaataaga gtttaattct
     4441 gttaatacta aatttttaaa tgcctattac tatagcatta attattatag gattggtagg
     4501 ttttctaatt aatacctttt gtatactctt ttaatagtca aatctttagc aattaaaaaa
     4561 cggggctaat tagccccgtt tctctttggc gatatttttt attctgcctt tttggtgtcg
     4621 cctacaaaag cgcgttgttt aagctcacgg tcgagcatga aaagtccatt gccattgccg
     4681 ttgattagct ttagcgtatc aattaattcc tgggcattgg cttcctcttc aacctgttca
     4741 tctacaaacc attgcagcat tccttttgca gcatggtctt tttcctcaat ggcaatatcc
     4801 ataaggttat tgattaagcg ggtaacctcc ttttcatgtt ttagtgtctc ctcaaaggcg
     4861 tggagagggg aatcccattt ggttggaacc tccttaatgg gcttgagctc aggcttactg
     4921 ccgcgctcaa ttaggtagtc gaaaaatttc atggcatggg ttagctcctc ctgccactgt
     4981 actttaaacc aattggcaaa gccattgaag ccctgcgctc caaagtgagc tgacatggac
     5041 aggtaaaggt atgccgacca aatctcggca tttatttgct cattgatagc ttgttctaaa
     5101 cgtttattca tagctttagt tttttaggtt ttatttcaaa tatagttaat tgctacttaa
     5161 ataacaatta atcgaggtca aggttcaatt gctgctttaa acggattagt gcaggattcc
     5221 gttctgccat aaagcggaac ttatcgtcgg gagtgtagag ccttggtttt ccgttatctt
     5281 caattttttc tacaatttcc tcagctattt caatgtttaa gattccaaaa cttttcttga
     5341 tgtaggtgag caaatctttt tttatctcgc taaaaagttc aatctggatt tgaccctcaa
     5401 actgaatagt gattttatta ttttctttta aaattggctt atacacctgc attaagctaa
     5461 aatgtgcggg tttatctttc tgaatttgtt cagcaaagct tgtccaagcc tgtagtattt
     5521 gttcgtgcga aattttttta atttcaacac ttgcaatccc ttggttttca gtttggattt
     5581 cctctacctt gggttcatca ttatttttaa tttcagcatt attcggtgct ccgtttaatg
     5641 cgcctttgat tgatgtggta cggataggtg ttttaggggt tgttggtatt gcttttggcg
     5701 tagatgttgg ttttacggta gatgatatag gctgactgta ctcaacggga tcggagtcct
     5761 ctacctggct gtcgtcagat tttttttttt gattaagccc acaaagctta ataagcatca
     5821 gctcaacatg aagcctttgg ttggggcttt gcctaaaggc aacatcaaac tggttggtga
     5881 cctgaagcgc ttcaattaag aaacctggtg ggcaggttgc tgccatctcc ttatactttt
     5941 gggcaatagc tgctccaacc tcgagtaagg gaattgtttt tggatcgtgg caaacaagca
     6001 cattccttag atgcatgctt agcccagtta taaactgttg ggcatcgaaa ccctttgaga
     6061 gaagctcatc gaatatctgc aatgagtcag cataatcgcc cttaaggaaa ttatcggtta
     6121 aacggaagta atactggtag tcgagaacat tcagattttc aattaccttt tggtaggtaa
     6181 tatggttctc gctaaagctc actacctggt caaagattga aagggcatcg cgcatggccc
     6241 catcggcctt ttgggcaatt atattcaagg cttctacctc atattcaaca ccctcttttt
     6301 tagcgatata ctcaaggtaa tttacggtgt cctcaacgcg gatacggtta aaatcgaata
     6361 tctggcaacg cgaaagaatg gtagggagga ttttgtgttt ttcggtggtt gccataataa
     6421 aaatggcatg ggctggcggt tcctcaagtg ttttaaggaa agcattaaat gcgctttgcg
     6481 agagcatgtg cacctcatcg ataatgtata cgctatactt accaatttgg ggcggtatgc
     6541 gaacctgctc aattaatttt cggatatcct caacggagtt gttcgatgct gcatcgagct
     6601 catggatatt gtaggagcgg ttggtgttaa acgatttgca gctttcgcac tcattacagg
     6661 cttccagctc aggggaaagg ttttggcagt ttatggtttt ggcaaaaatt cgggcacatg
     6721 ttgttttgcc tacccctcgt ggcccgcaga ataagtaggc ttgggctaac tgcttacgct
     6781 gaatggcgtt ttttagggta gtagtaattg atgcctgtcc aaccacgctt ttaaacgttg
     6841 ctgggcgata ctttcgtgcc gatacaatga aattctccat tccgccaaaa tttcaaaaac
     6901 aaaaatagga aaatacttaa actaacagtg gagtattaac tgttaaatac tataatttac
     6961 agaatgtgag ggtaataagt tattaacagg gatttttgaa ttaataaaaa aaacagctat
     7021 ggtggaacgg gatgctcaaa atgaaataaa actacctttc tgatttcttg aatcttacta
     7081 caagcatata cagtatcaca gtgttaaacg gggtaacaca aagttacaca gtgtaaggtt
     7141 ttatatcccg ttgcagcaca atcaactttc aatcattaac tagcttgtca tcctgagcgt
     7201 agcgaaggat ctcaaacccg acagaagggc aaaagagcaa agcgatactt cgactgggtt
     7261 cagcatgaca atagtttcac gaacaacgag caactataca cgaacaactt catttgtcat
     7321 cccgagcgta gcgaaagatc ttttgaatag tgtgcagtgt acagtgtgca gtttacaggg
     7381 aatatggaga gtagtatttg gttttcagtg tttagtgtaa tgcttttttt cactatcaac
     7441 catcaaccat caaccatcaa cttatccttt aacctttatc ctttatcctt tatcctttat
     7501 cctttaccct tatcaccgct atctccggtc tattcccaag ccttaccctt gggccccagg
     7561 ttccatagcc gggcgataca tatatgtgtg tattgccaat ctttttatat cccatgctaa
     7621 cctcatatac cgctcgggta atgtagttta aaggccacat ctgcccatga tgggtgtgac
     7681 ccgaaagctg caagtcagct ccggattcag cggattcgtt tagattgaat ggctgatggt
     7741 cgagcactat tgtaaactta ctctggtcga tatcttttgt taattccgat aaagttttac
     7801 ggctttgccc caaggcaaaa ttgctttgcc ggtcgtaacg gcctacaatg ttgatgttgg
     7861 caacttcagc aaccgtgtcg ttcaaaacgt ttacaccatg tttttgaagg tactcaatta
     7921 ttgcctgatg gttactaaag aattcatggt ttccggttac tgcaaaaacg ccgtattttg
     7981 cattaatacg ctcaaattgc tctcccattt gaccatttac caccggggca atgtcctcat
     8041 caaaaatatc acctacaaat aagacaatat caggcttttg tgagttgatt ttgtccacaa
     8101 gactgcttaa ttcatttttc ccaattattg ttcccaggtg aatatcggaa accattgcta
     8161 tggttaagcc tccacctgga acaggtttac ttgattcaat ttttagctgg tttatcttgg
     8221 ggttgacagc gtttatatag ccagcaagaa tgactgtaag cgttgcaaag tataccattc
     8281 caactatcat tctatgtgtt tcgagggagc ttttaatatc tatttttact aaccaattta
     8341 taagaggcga tgttatttga aaaagtagga aaaaaagtgt taggtaaagc ataaaggcta
     8401 accaaattga gccaaccttt acaagaagtg ttggaatttg tccacctaat atagcttcaa
     8461 gggttctacc taaagggtag gagagtacaa taaaccaaaa tacaaacttt aaccaagttc
     8521 caattgaagc taagctgaat accggtcggg tttggtaaaa tatgaataaa ttaacaagcg
     8581 cgtaaacgct tagaacaatt gatagaaaaa tggctatttg aggaattttc atgttttcat
     8641 ttttttcaat taacaaaatt agcatttgaa agttcatggt aaaaaaaaat cccggtaaaa
     8701 ccgggattta aacaagtatg ttggataatc taagtcaatt ttacgtctac tgcattcagc
     8761 ccttttctac cttgttcaag gttgaaagta acctcatcgt tttctgaaat ttgatctttt
     8821 aatccagtgg cgtgtacaaa atactcttca cctgtttctg aatctttaat aaaaccaaag
     8881 ccttttgatt cattaaaaaa ttttactgtt cctttgttca tttgttgtga tttattaatt
     8941 cgggtgcaag taacgcataa tatttggatt tagcaccatt aatttagatt tattttaaat
     9001 atatttctta tgtaattgta tgtcaaatag ataacatttg tttaattgtg ttaattgttt
     9061 caaaaatatt tcgattcggc gaggtattag ttccattgat gctttgtgct tattcctttt
     9121 cactttaact ttttgggttt atcacccata ataaatgtta tttcaccacc acttactata
     9181 tcgttatggt ttaaaaagta actattgtaa ggtttgccat taagcaacac cttctgaaca
     9241 taaacattgt ttttagcttg attttgggtt gaaatggtaa gtgtttttcc gttttccagt
     9301 ttaattgtgg ctttggtaac caatgggctg ccaatagcat agttgggcga tgcaggtgct
     9361 acaggataga aaccaagggc gctaaaaatg taccaggcac tcatctgtcc gcagtcatca
     9421 tttccgctta atccgtcagg tttgggttgg tacattaggt tgcaaatggt tctaacccag
     9481 tactgcgttt tccaagggtt atcagtccag ttgtaaagat atggaacatg gtgcgatggt
     9541 tcattcccat gaacgtaatt gcctatcaat ccatcgcggg ttatgtcttc ggtttcggcg
     9601 aagtattcat ccggaagatg catggtaaag agagtgtcaa gataggctcg gagttgcttt
     9661 ttcccaccaa gcatgcttac caaagcatcg gggttatggg gtacataaag gctatagttc
     9721 catgagttac cctcaataaa cccttgtcca tgtgtgctaa gcacgttaaa actagttttg
     9781 aaactcccgt ttgataaacg aggacgcata aacccaatgg acttatcgaa tacgttttga
     9841 aagttgtttg aacgacttgt gaactcgttg taaatatctt ctctatccag ttttttagca
     9901 agttgtgcaa tgcaccaatc gtcgtaagcg tattcaaggg ttttggaaac agaactcccc
     9961 gatatatcct caggaacaaa accaaattct ctgtaatagc ctaacccatc gtaccagtta
    10021 ttcttagcgc tttgcacgca agccataagc gcatagggta aatcaaagcc atcgttgcct
    10081 ttaacggctg cgtcggcaat cactgatacg gcatggtatc caatcataca ccagttctca
    10141 tttgcgtagt gcgaccatat agggagcatg cgatggacac tttgactgta atgagccaac
    10201 atcgatttta ccatatcgac atttcgtttt ggctgtataa tattaaaaag cggatgcaaa
    10261 gctctatatg tgtcccagag tgaaaaggta gtgtagttat caaaattggt tgctgtgtaa
    10321 atgttttggt caagccctcg atatttccta tcagtatcca tgtaaatggt tggacttaga
    10381 aaggcatggt acatcgatgt gtaaaagttt accttgtcac tatcagtaag ggtttcgact
    10441 tcaattttac ctagctcatc attccattgc tcttgaccct cttttttgat ggcatcaaaa
    10501 tcccagcctg gtgcttcggc atttagattt tcaactgctc cgctggtact cacgggcgat
    10561 aatgcaactt ttagcaatac ttgttcatgg tttgcagttg aaaagtcgaa gtggcaacga
    10621 atttcacgcc cagcattttc agggaaattg gtgttttgat tgaattttcg ccaaaaacct
    10681 ttataaatca acttatcgcc attgattatg ccataggttt taaatggttt tgaaaaggta
    10741 atggcaaagt atacagtgcg ggtgcgtgcc catcccgatg tttgtcggta cccggttata
    10801 agggtatcgt tttcaacccg aatgaatgtc caaacatttt tttcgggata attataaatt
    10861 ccatgaacaa ggtcaatgat tatgtgcgct gagtcggttt cagggaatgt gtaccgatgt
    10921 agcccaaccc ttgtggtggt agttagctct gcaagaatgt tataatctaa gagtttgacc
    10981 ctgtagtagt ttggttcggc aaactcattg gaatgagaaa atttggagcg ataccctgta
    11041 tttgtattgg aagcagttcc ggggtttaag tgaagcttgc caaccgttgg catcacaaga
    11101 atatcaccaa ggtcggaatg ccctgttccg ctaaaatggg tatggctaaa gccaattatg
    11161 gtactatccc cgtactgata accagcacaa tacttgtaaa catcgggatt gtacttgcca
    11221 ttggaagagt aggggatagt atcggtatcg ggactaagtt gcactgcccc aaacggaacg
    11281 gttgcgcctg ggaaagtatg ccccattcgc tcagttccaa tgaatgggtt tacccatttg
    11341 atataattct taggttgtgc aaaaattgca caagttgaaa aaagtaaggt aattgttagt
    11401 gtagagcaat actttcccat tttctaactg gctaaaattt cagttaaaat aaatatttat
    11461 ttcactactg aacgggtaaa aaataaaata aggggttacc gaccgaaggt ttcgccccat
    11521 gttgtagttt tgttttactg aactaaacat acaacttggg caaaaatacg gaaattaaat
    11581 ttgtcggaca gcctattttc aaacgagttt gattatctga tatttagaac ccccgctcgc
    11641 gcggatttac cttcggtgag ctcccttcgg tcggttgtaa tccgtgcggt tgtgttttca
    11701 atgaagcaca gcttgaataa gtaataaatt atgtaagagg tgtttgtcac ggactacaag
    11761 tccgtgccag caatgagtga cgtatgccgc acgcgtt
//
LOCUS       DAOSAL010000076        11449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_3781_length_11449_cov_51.170967, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000076 DAOSAL010000000
VERSION     DAOSAL010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 11449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11449
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3781_length_11449_cov_51.170967"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="PK049_09630"
     CDS             <1..318
                     /locus_tag="PK049_09630"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOK61857.1"
                     /translation="AARSEQRVAELKMDEAKELIGLQVQQAIFQLNESIKKVEMAQVN
                     LKQAEENLKVARDAFESGRQKTSDVLEAQAMWQNAFSDLIDARMEYRLNVVNLKRVTG
                     SLK"
     gene            383..1426
                     /locus_tag="PK049_09635"
     CDS             383..1426
                     /locus_tag="PK049_09635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOK61858.1"
                     /translation="MKRLILLFTAAAALVFVSCSGNKQEQAQSEVNVPLVSTIRVEER
                     TFNPVLTFTGTVFAYKEANLGATLPGKVEKIYFEEGQMVKKGDLIVELSDEMLTQALI
                     EHETIKKDFERVSRLREKGSISEMEYDHVKAQYDASTAKVEMVKKNTQVYAPFSGIIA
                     ERMMEEGEVYFLNPGLEPGYSMRSGIVRLMQIDPATVKFDVNEKDLIHIRKGLPVVVK
                     LDGMPGKTFQGKVDRIKPMLSTTTRSTSVEVTIPNSNMEVKPGMFAFVDVMLPEVKSA
                     VVPLRSIYRMPGTSDDFIFTVVNDTVHRVKVERLQTIGEHVAVRGIDAGTEIILEGKN
                     RVNDGLKVKVSNR"
     gene            1450..>3182
                     /locus_tag="PK049_09640"
     CDS             1450..>3182
                     /locus_tag="PK049_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOK61859.1"
                     /translation="MKLPQVAVKRPVFTAMLFVAILLFGVVSLTRLPLDIMPEMELPT
                     LTVITVYPGASADEVEEQVSKKLEKILSGTEHLKEITSQSKENVSFVQLQFEWGTDIT
                     SAANNARDLIELVKNKLPRDAHNPIIFKVNSSLMPVLVYGVTANESYNGLYKIVDDEV
                     ASKLRKVPGVGSVIVIGAPEREIKVDIDPVKLHAYNLSLNQVATVLKAQNISIPGGNI
                     KVGNDDYSVRIPGDIASVDELRDMALISFNGKVIRLSDVAEVRDEYKEKDDVGRTTNG
                     KGVTLMVQKQSGENTLEVVKKIRKKVAEIKPALPSDVSITEVMKSDELITESINNLSE
                     SLWYALFFVVLVVMAFLRNWKQSFIIFITIPFSLIVAFIVMFASGWTINIFSLMSLVV
                     ASGMVVDNAIVVLENITQHIEKGAKPKQAAIFGSSEMGMAISASTLTTIVVFLPMIFM
                     GGIVGIMFKQLAILTSVTLIASLFTALTLTPMASSQMLEGMKKNEKRRHGKLFEWSER
                     MFQKLENGYKKALAWVVHHKTFTVIVTVALLAFSLWVGKGLGTDYIPEFDAGDVVAVI
                     ETEVGTSTTKT"
     assembly_gap    3183..3282
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3283..4429
                     /locus_tag="PK049_09645"
     CDS             <3283..4429
                     /locus_tag="PK049_09645"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOK61860.1"
                     /translation="LLGGSIISAAVTGNKKPIEIEVSGNSFGSINSTAEAITARMRQS
                     KYFSDVINNVDKGKLEVQIRVDKRKASAMGLNSAMIGLQVRQSIYGTEAGEFKEEGDE
                     YDITLRYSPENRNDINQIRNIQLKNLLNQTIPLSAVAEVEMGTSPLQINRKGQQRVVK
                     VMAELNDVSLGDAHKEAKRILAGLDIPSDVTVEIAGQTTDQGESFGDLYLILALGVLL
                     VYMVMASQFESFRDPFIIMFAVPVTFIGVIWAFKLIGLTLSITTFVGLIMLMGIVVNN
                     GIVLVDYTNLLRKRGYKLYDAIAEAGRSRLRPVLMTTFTTLLGMVPMATSSGMGREAY
                     SPLGITMISGLLVSTLITLLLVPTIYAIFHDNERKAEVDTNKSVLFN"
     gene            4443..4748
                     /locus_tag="PK049_09650"
     CDS             4443..4748
                     /locus_tag="PK049_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61861.1"
                     /translation="MKMIYCTCNVAVLEPLLKMLDDNNVRDFQVVEQVTAKNKKGDHR
                     FNNPVWPGYNSTVFIQITEDEKAKMVMQKIREFNRNAFNDNELVTACMWSIEDYFFD"
     gene            5116..6069
                     /locus_tag="PK049_09655"
     CDS             5116..6069
                     /locus_tag="PK049_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HOK61862.1"
                     /translation="MNIIKFSAQFPVEKSCIEHFKAQRDACGVVCPKWGCVHPYWLQN
                     KLCYECKQCHHRQSLRSGTVLENSKLPFMYWYAAMHLLTSTKKSYSAAELQRQLGHKR
                     YQPIWEMVCKLRDVMGKRYNLYQLTDEVELDNAFIATLIAEEEKDKPLKRGIGSQRQP
                     KAVVVNESSFVENPKPGKKPKRVNHLKMLVIEDLRADTISKTVNEHVNPKAELTTNDA
                     TPYSKLFEQVKSHHAQAIKKEDLPKTLPWVNIAIGNVKRLLLDVHHQLTNEYLQYYLN
                     EFCYKFNRRYFGDAFFDGFLIAAVSYNTDFRSRIYNRNSCG"
     gene            6090..6902
                     /locus_tag="PK049_09660"
     CDS             6090..6902
                     /locus_tag="PK049_09660"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOK61863.1"
                     /translation="MRKIGLYISVLLSILNYACTDLMEYSPYEGKVQEGSRSLNQTAI
                     GKLQLQSSKTFKPFTFAIIGDSHTYYDDFIKQISVLNNLDSIDMVIHMGDITLSGIYR
                     EFLWYRDIIKPLKYPLITIIGNHDYLSNGEYMYRDMFGPTNFSFTYNNCLLVFFDDIT
                     WERNTKDPDFEWLKSTISATATYTHKFVFTHIPPWDEQFSHGNELYYNYLLAKNNVDY
                     SIHGHIHRFYHDKHYADLYGNVNYLACGDSQDHEVVLVKVKENNIDVEVIHF"
     gene            6905..7546
                     /locus_tag="PK049_09665"
     CDS             6905..7546
                     /locus_tag="PK049_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61864.1"
                     /translation="MKTNRFLLLGLLILLYHPRLHAEDPVKGLRQKITEEKKIVLKND
                     TVTIKPYWFMPTQFKVQYAGSIGFVSFGAGYDFRNGYQPTLLYGFLDNNFGGSNITVH
                     TISLKNRFKLTQNPLLGCFTPIAGISVNWGITNNTFIRLPPHYPENYYFQNKIHLSPF
                     WGGEFNFSVAQKNLGVYFEFSTLDAYLLEYFRTDFVKLNKIWSLGMGITLYLN"
     gene            complement(7538..8839)
                     /locus_tag="PK049_09670"
     CDS             complement(7538..8839)
                     /locus_tag="PK049_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HOK61865.1"
                     /translation="MIKRIDNYFELSKANTSIRTEVLAGITTFMTMAYILAVNPSILS
                     NTGMSRDALFTTTALASFLATMIMAIWARLPFALAPGMGLNAFFAFTVVLGMGHSWQF
                     ALTAVFLEGLIFIALTFLNIREKIIDAIPLGIKHAISAGIGLFIAFIGLQNAGVIVKN
                     DNVMVGLGQMGSPSVLLCLAGVIVTGVLLARKVKGALLIGIFLVTLAGIPFGLTELPQ
                     GGLFSMPPSVEPIAFKFEFHTILSIDMLIVLFTFLFVDMFDTLGTLVGVATKANMLDE
                     KGKLPRARQALFADAIGTTLGAMLGTSTVTTYVESASGVAEGGRTGLTSFTTAMLFIA
                     ALFFSPMFLMVPAAATAPALILVGSFMLSPILKIDFNDVTEAIPAFFTIIMMPLTYSI
                     AEGIVFGMLSYVLLKAFTGKVKQVSLVMWVLSLFFLLKFLV"
     gene            complement(8929..9975)
                     /locus_tag="PK049_09675"
     CDS             complement(8929..9975)
                     /locus_tag="PK049_09675"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HOK61866.1"
                     /translation="MNFFASHWGSIDGKDVMKYSISTEGGLEVSISNLGAAMVAMHLL
                     NDENEPLNLAYGLLRPSDYLKADYYPGVIVGRYANRIANAKFTIGSDEYALCANEGKN
                     ILHGGKIGFSHRVWETVMFEEHNGVCMLEFYLNSLHGDQGFPGNVDVWVIYQITDDNQ
                     VSISYRAVTDRPTHLNLTTHGYFNLSGFETDVLDHMLRIDANHYLPVNSELIPTGEIR
                     EVDGSPFDFRKFKAVGADTDLVGTCYDHCYMLNNPTIDNPSVVLFNPKTHIGLTIYTT
                     QPGIQLYTPMNKPNIANPMIKLPEKGNWAVCMEPQHFPNSPNEGRFPTTLLLPGQEYD
                     HTTILSVTMGGVDK"
     gene            complement(9979..10308)
                     /locus_tag="PK049_09680"
     CDS             complement(9979..10308)
                     /locus_tag="PK049_09680"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOK61867.1"
                     /translation="MEVDMPVSEITFWKWFLPKLRNKFFLTVFIFLIWMLIFDSSNWV
                     EMLAAKRRIKSLEEEKVYYLQKIEEDKKKIKELRTNAENLEKFAREQYLMKKPNEDIF
                     IVDETDL"
     gene            complement(10298..10507)
                     /gene="thiS"
                     /locus_tag="PK049_09685"
     CDS             complement(10298..10507)
                     /gene="thiS"
                     /locus_tag="PK049_09685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HOK61868.1"
                     /translation="MDITLNNLPEHIDTNKAAITVNELLAFKHFSFKMLVVKVNGKLV
                     KKDEYSLATIKDGDDVQVIHLISGG"
     gene            complement(11031..11213)
                     /locus_tag="PK049_09690"
     CDS             complement(11031..11213)
                     /locus_tag="PK049_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61869.1"
                     /translation="MGAPRVELPRDTIRHACGVTHVVSLTKPTATFHPRPITGSGFYA
                     TACFFRSIPSQGKGLW"
BASE COUNT         3269 a         2463 c         2560 g         3057 t
ORIGIN      
        1 gcagcccgaa gcgagcagcg tgtggccgaa cttaaaatgg acgaagccaa ggaacttatt
       61 ggcttacagg tgcagcaggc catttttcag ctcaacgagt caatcaagaa ggttgaaatg
      121 gcacaggtaa acttaaaaca ggccgaggaa aacctaaagg ttgcccgcga tgcctttgag
      181 agtggccgcc aaaaaacttc cgacgtgctt gaggctcaag ctatgtggca gaatgctttc
      241 agcgacctga ttgatgcccg catggaatac aggctgaatg tggttaatct taaacgcgtt
      301 acaggaagtt tgaaatagac tggttaaacg cacttctgaa attttgaatt tggaattttg
      361 aatcaattaa aaatattaaa ccatgaaacg attaatttta ctatttacag cagctgctgc
      421 gttggttttt gtatcgtgca gtggcaataa gcaggaacaa gcccaaagcg aagtgaatgt
      481 tcctcttgta tccaccatca gggttgagga gcgcactttc aatccggttc taacctttac
      541 aggtacagta tttgcctaca aggaggcaaa cttaggcgct actctacccg gaaaggttga
      601 aaaaatctac tttgaggagg gccagatggt taaaaagggc gacctcattg tggagctttc
      661 cgatgagatg cttacccagg cgttaattga gcacgaaacc atcaagaagg attttgagcg
      721 tgtatcgcgc ttgcgcgaga agggaagcat cagcgagatg gagtacgacc acgttaaggc
      781 ccaatacgat gcttcaaccg caaaggtcga aatggttaaa aagaacacac aggtttacgc
      841 tcccttcagc ggcataattg ccgagcgaat gatggaggaa ggagaagtgt acttccttaa
      901 ccccggtctg gaacccggct attccatgcg ttcgggcatt gtaaggctga tgcaaattga
      961 ccctgccact gtaaagttcg atgtgaacga aaaggattta atccatatcc gtaaaggcct
     1021 acctgtggtg gttaagctcg atggtatgcc cggaaaaacc tttcagggaa aggttgatag
     1081 aattaagccc atgctctcaa ccacaacccg ttccacatcg gttgaggtta ccattcccaa
     1141 ctcaaacatg gaggttaaac cggggatgtt tgcctttgtt gatgtaatgc tacccgaggt
     1201 gaaatcggca gtggtgcccc tgcgtagcat ttaccgtatg cctggcacat cggacgattt
     1261 tatatttact gtggttaacg ataccgttca ccgtgttaag gttgagcgcc tgcaaaccat
     1321 aggtgagcat gttgccgtta ggggtatcga tgcaggtacc gaaattatcc tcgaaggcaa
     1381 aaaccgcgtg aacgacggtt taaaggttaa ggtgagcaac aggtaaaaat cctaaaaaaa
     1441 cggaagaaca tgaaactacc acaagttgcc gtaaaaaggc ctgtattcac agccatgttg
     1501 tttgtggcaa tcctgctctt tggcgtggta tcgcttacca ggctaccgct cgacattatg
     1561 cccgaaatgg agttacccac tcttacggta attactgtat atccgggcgc cagcgccgat
     1621 gaggtggaag agcaggtatc caaaaagttg gaaaaaatcc tatcgggaac ggaacaccta
     1681 aaggagataa catcgcaaag caaggagaac gtatcgtttg ttcagcttca gtttgagtgg
     1741 ggaaccgata ttacttcagc agccaataat gctcgcgacc ttattgagct ggtaaagaat
     1801 aaactccccc gcgatgctca taacccaatt attttcaagg taaacagttc actgatgcct
     1861 gttttggtgt atggcgttac cgccaacgag agctataacg ggctttacaa gattgttgac
     1921 gatgaggtgg ccagcaagct acgcaaggtg ccgggagtgg gctcagtaat tgtaataggc
     1981 gcacccgaac gggagattaa ggttgatatc gaccccgtta agctacacgc ctacaacctg
     2041 tcgctcaacc aggtggcaac cgtgctaaag gcacagaata tttctatccc cggtggaaat
     2101 ataaaggtgg gcaacgatga ctactcggtt cgcattcccg gcgatattgc cagtgttgat
     2161 gagcttcgcg atatggctct aatcagcttc aacggtaagg ttattcgcct tagcgatgtg
     2221 gccgaggtgc gcgatgagta taaggagaag gacgatgtgg gaagaaccac aaacgggaaa
     2281 ggggtcaccc tgatggtgca aaagcaatcg ggtgagaaca cccttgaggt ggttaagaaa
     2341 atacgcaaaa aggttgctga aattaaaccc gccctaccat cggatgttag cattaccgag
     2401 gtgatgaaga gcgacgagct tatcactgaa tccatcaaca acctttccga atcgctctgg
     2461 tatgctctgt tctttgtggt gcttgtggtt atggcatttc tgcgtaactg gaagcagagc
     2521 tttatcattt tcataacaat acccttctcg ctgattgtgg cattcattgt gatgtttgct
     2581 tcagggtgga caatcaacat cttcagcttg atgtcgttgg tggtggcatc gggtatggta
     2641 gtggataatg ccattgtggt gctggagaat ataacccagc acattgagaa aggggctaag
     2701 cccaagcagg cagcaatttt cggtagtagt gagatgggta tggcaatttc agcatcaacc
     2761 cttaccacca ttgtggtatt cctgcccatg atattcatgg gtggcattgt gggtatcatg
     2821 ttcaagcagc tggccatact aacttcagta accttaatag cttcgttatt cacagcgctt
     2881 accctaaccc caatggcatc gtcgcaaatg cttgagggca tgaaaaagaa cgaaaaacgc
     2941 cgccacggaa agctattcga gtggagtgaa cgtatgtttc agaaacttga aaatggctat
     3001 aagaaagcat tggcatgggt tgttcaccac aaaacattta ctgttatagt aacggttgcc
     3061 ctgctggcat tcagcctttg ggttggtaag ggcttgggaa ccgactacat ccctgagttc
     3121 gatgctggcg atgtggttgc agtaattgaa accgaggtgg gaacatcaac caccaaaacc
     3181 gannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncttgcttg ggggtagtat
     3301 catctcagct gctgttacag gaaataaaaa acctattgag attgaggttt caggcaacag
     3361 ttttggcagt ataaacagca cagccgaagc cattactgcc cgcatgcgcc aatccaaata
     3421 ctttagcgat gttatcaata acgttgataa gggaaaactc gaggttcaaa tcagggttga
     3481 taagcgcaag gcttcggcca tgggcctgaa ctcagccatg attggcctgc aggttcgcca
     3541 aagcatttac ggaaccgaag ccggtgagtt taaggaggaa ggcgatgaat acgatatcac
     3601 tctccgctac tcgccagaga accgtaacga tatcaaccaa atccgcaaca tacagcttaa
     3661 aaaccttctg aaccaaacca taccgctatc ggctgtggcc gaggtggaga tgggaaccag
     3721 cccactccag attaaccgta aagggcaaca acgtgtggtt aaggttatgg ctgagctaaa
     3781 cgatgtttcg ctaggcgatg cacataagga ggctaaaagg attctggccg gcttggatat
     3841 tccaagcgat gttaccgtag aaattgctgg ccaaaccact gaccaagggg agtcatttgg
     3901 cgacctttac ctcattctgg ctcttggcgt gttgcttgtt tacatggtaa tggcatcgca
     3961 gttcgagagc ttccgtgacc cattcatcat tatgtttgct gttccggtta cctttattgg
     4021 ggtcatttgg gctttcaagc taatcggttt aaccctcagt atcacaactt ttgtgggttt
     4081 aattatgctt atgggtattg tggtgaataa cggtatagtg ctggttgact acaccaacct
     4141 gctacgtaag cgtggttaca agctttacga tgccattgcc gaagcaggcc gaagccgttt
     4201 gcgtccggtg cttatgacta catttacaac gcttttgggc atggtgccta tggctaccag
     4261 cagcggtatg ggacgcgagg catactcacc acttgggatc accatgatta gcgggctact
     4321 ggtttcaacc cttatcaccc tgctgcttgt gcctaccatt tatgccattt tccacgataa
     4381 tgagcgtaag gccgaagtgg ataccaataa atcggttttg tttaactaaa ttgatgttag
     4441 ctatgaagat gatatattgc acctgcaacg ttgctgttct tgagccatta ctcaaaatgc
     4501 ttgacgataa caacgttcgc gactttcagg ttgtggagca ggttacggcc aagaataaga
     4561 aaggcgacca ccgttttaac aatcccgttt ggcccggata taactctacg gtatttatcc
     4621 aaatcaccga ggatgagaag gctaaaatgg ttatgcagaa aatccgcgaa ttcaatcgta
     4681 atgcatttaa tgataatgaa ctggttactg cttgtatgtg gtccattgag gattactttt
     4741 tcgattaatt ttttacgttg ttaaatcacg ttaaagcccc cagttaattg ggggctttaa
     4801 taaaatttat gattatccgc tttagtaaca atattatttt tacgttattg cgatccaggc
     4861 gttgtttgat gggagaagca atcattggca ttgatttaca ataaattgct tcttctggaa
     4921 aagtactccc tgcctgccag caaaggcagg gcaaggcaag ggcaggctcg caatgtcaaa
     4981 ttcattatta ttttgttgta tctacttaaa aagggataaa ttacgatata cttttcagaa
     5041 aatagttgtt aaaaatatgt ataattcact ggtattcatc gtatttatgt cataagatca
     5101 atatctatag ttcttatgaa tatcattaaa ttcagtgcac aattccccgt cgaaaagtct
     5161 tgtatcgagc atttcaaggc tcaaagagat gcttgcgggg tcgtttgtcc caaatggggt
     5221 tgtgtgcacc cctattggct gcagaataag ctgtgctacg aatgtaaaca atgtcatcac
     5281 cggcaatcct tacgctcagg aacagtgttg gaaaacagca aattaccttt tatgtattgg
     5341 tatgctgcca tgcatctgct taccagcacc aaaaaatcgt actctgctgc agaactgcaa
     5401 cgacaattag gccataaacg ctatcagcct atttgggaga tggtatgcaa attacgcgat
     5461 gtgatgggga aacgctataa tttgtaccaa ttaaccgatg aggtagaact cgacaatgct
     5521 tttatcgcta cattaattgc ggaggaagaa aaggataaac ctctcaagcg gggcatagga
     5581 agccaaagac aacccaaagc agtggttgtg aacgaaagtt ccttcgtgga aaatcccaaa
     5641 ccgggcaaaa agcccaaacg tgtcaatcat ctaaaaatgt tggttatcga agatttaagg
     5701 gctgatacca tttcaaaaac tgtcaatgaa cacgtaaatc caaaggcgga attgaccacc
     5761 aatgatgcaa ctccttacag taagttgttc gaacaggtca aatcacatca tgcgcaggct
     5821 ataaagaaag aagacctgcc aaaaacatta ccatgggtaa acattgccat tggcaacgtt
     5881 aaacgattgt tgttggatgt acaccatcag cttactaatg aatacttaca atactatctc
     5941 aatgagttct gctataaatt taaccgacgt tattttggtg atgccttctt cgacgggttt
     6001 cttattgctg ctgtaagcta caacaccgat tttaggtcaa gaatttacaa taggaattca
     6061 tgcggataat catttaaaat ttttaagcaa tgcgaaaaat tggtctttat atcagcgttt
     6121 tactttcaat tttaaattac gcttgcaccg acctaatgga gtatagcccc tacgagggaa
     6181 aggttcagga aggctcaaga agtttaaacc aaacagcaat tggtaaactt cagttgcaaa
     6241 gctctaaaac atttaagccc tttacatttg ccattatagg cgacagccat acctattacg
     6301 atgattttat caagcaaata tcggtgctca ataacctcga tagcattgac atggtaatac
     6361 acatgggaga tatcacccta agtggtattt atagagaatt tctgtggtac agggatataa
     6421 tcaaaccgct gaaatatcca ttaatcacaa ttattggcaa tcacgattac ctatcgaatg
     6481 gcgaatacat gtatcgcgac atgtttggcc ccaccaactt ttcgtttacc tataacaatt
     6541 gcctgcttgt gttttttgat gatatcacct gggaacgtaa tactaaagac cctgactttg
     6601 aatggttaaa atcaaccatc tcagctacag caacctacac ccataaattt gtgtttacac
     6661 acatccctcc gtgggatgaa caatttagcc atggtaatga gctatattac aactacctgc
     6721 tcgcaaaaaa caatgtcgat tactcaatac acggacatat tcatcggttt taccatgaca
     6781 agcattacgc tgatctttat ggcaatgtaa attatcttgc ctgtggcgat agccaggacc
     6841 atgaagttgt tctggttaag gtaaaggaaa acaacataga tgttgaagta atccactttt
     6901 agccatgaaa acgaataggt ttttactttt gggattattg attttgcttt atcatccaag
     6961 gctacatgct gaagacccag taaaagggct aaggcaaaag attaccgagg agaaaaagat
     7021 agtgcttaaa aacgatactg taaccataaa gccatactgg tttatgccaa cgcaattcaa
     7081 agttcagtat gcagggagta taggttttgt atcttttggt gcgggttacg atttccgtaa
     7141 tggatatcaa ccaactttac tatatggttt cctcgataat aactttggag ggagcaacat
     7201 aaccgttcac accatatctc ttaaaaatcg atttaagctt acacaaaatc cattacttgg
     7261 atgttttaca cctattgcgg gaatatcggt aaactggggc ataaccaata atacgtttat
     7321 ccgcttgcca ccacattacc cggagaacta ctattttcag aataaaatcc atttatctcc
     7381 tttttgggga ggtgaattta atttttcggt agctcaaaaa aacttgggcg tttactttga
     7441 gttctcgacc cttgatgctt accttttgga atattttaga accgattttg taaaactgaa
     7501 caaaatttgg agtttaggca tgggtataac gctttaccta aactaaaaac ttcagcaaaa
     7561 agaaaaggga taaaacccac ataaccaagc taacctgttt tacctttccg gtaaatgcct
     7621 taagcaaaac atacgatagc atgccaaata caattccctc ggcaatgcta taggttaatg
     7681 gcatcattat aattgtaaaa aaggcaggaa ttgcttcggt tacatcatta aaatcaattt
     7741 taagtatagg gcttagcata aaagaaccca caagaattaa tgcaggagca gttgctgctg
     7801 caggaaccat taaaaacatg ggcgaaaaga aaagtgcagc aataaaaagc atggctgtag
     7861 taaatgaagt caaaccagtc cgaccacctt cagcaacccc cgatgcgctc tcaacatagg
     7921 tagtaacagt gcttgtgcca agcatggccc caagagttgt gccaatggca tcggcaaaca
     7981 gggcttgcct agcacggggt agtttgcctt tttcatctaa catgtttgct tttgtggcta
     8041 cgccaactag ggttcctaaa gtatcgaaca tgtcaacgaa taaaaaagta aaaagcacta
     8101 tcagcatatc gatactcaaa atagtatgaa actcaaactt gaatgctatt ggttcaactg
     8161 aaggtggcat actaaataat ccgccttggg gtaattcagt taaaccaaag ggaatgcctg
     8221 caagggtaac caaaaaaatc ccgataagca aagccccttt aaccttgcga gccagtaaaa
     8281 caccggttac aatcacaccg gcaaggcaca acaaaaccga tggggaaccc atttgcccca
     8341 atcccaccat cacgttatcg ttctttacaa tcacccctgc attctgaagg ccaataaagg
     8401 ctataaacaa ccctatgcca gccgatatgg catgtttaat tccaagaggg atagcatcaa
     8461 ttattttctc ccggatattt aagaaggtta atgcaatgaa tatcaaccct tccaggaata
     8521 cggctgttaa agcaaactgc cacgagtgcc ccattccaag cactaccgta aaggcaaaaa
     8581 aggcattaag tcccattcct ggcgccagcg caaaaggcag ccttgcccaa attgccatta
     8641 tcatggttgc caaaaacgat gccagggctg ttgtggtaaa aagtgcatcg cggctcattc
     8701 cggtattcga caatatagaa ggattaaccg caagaatgta ggccatggtc atgaaagtgg
     8761 ttataccagc tagaacctcg gttcttattg acgtgttagc cttactgagt tcaaaataat
     8821 tatcaatccg cttaatcatt ggtttttgga acaactgatt ctttcgcctg ttgattgaaa
     8881 aggtcattca aatatcaaac ccaatacaag gtgtataaat tcagtagatt atttatcaac
     8941 gccccccatg gtaactgata gtatggttgt atggtcgtat tcctgaccgg gtagcaaaag
     9001 cgttgtaggg aacctaccct cgttaggtga gttggggaag tgttggggtt ccatgcatac
     9061 cgcccagtta cctttttcgg gtagttttat catggggttg gcaatatttg gcttattcat
     9121 aggggtgtaa agctgaatgc cgggctgtgt ggtgtaaatg gttaacccta tatgggtttt
     9181 ggggttgaac agcaccaccg atggattgtc aattgtgggg ttgttcagca tgtagcaatg
     9241 gtcgtaacat gttcccacca ggtcggtatc ggccccaacg gccttaaact tccggaaatc
     9301 gaagggtgaa ccatcaacct ctcggatttc gccggttgga ataagctcgc tgttaaccgg
     9361 caggtaatgg ttggcatcga tgcgcagcat gtggtcaagt acatcggttt caaacccgct
     9421 caggttaaaa tagccgtgag tggtaaggtt caggtgggta ggcctatcgg taacagcacg
     9481 gtagcttatg ctcacctgat tatcatcggt aatctggtaa attacccaaa catcaacgtt
     9541 tcccggaaat ccttggtcgc catgcaggct gtttaggtaa aactccagca tgcacacccc
     9601 attatgctcc tcaaacatta cggtttccca aactctatgg ctaaaaccta ttttcccgcc
     9661 gtgcaggatg tttttaccct cattagcgca tagcgcatac tcatcggaac caatggtaaa
     9721 tttggcgtta gcaatacgat tggcataccg cccaacaatt accccagggt agtaatcggc
     9781 cttcaggtag tccgatgggc gtaaaagccc ataagccagg ttaagcggtt cgttctcatc
     9841 gttcaggagg tgcatggcca ccatggcagc acccaggttc gatatggata cctccaatcc
     9901 cccctcggtg gatatggagt atttcattac atccttacca tctatgctac cccagtggct
     9961 ggcaaaaaag ttcataggct acaaatcggt ttcgtcaaca ataaaaatat cctcgtttgg
    10021 ttttttcata agatactgtt cccgcgcgaa tttttcgagg ttttcggcat tggtgcgtaa
    10081 ctccttaatt ttctttttat cctcttcaat cttttgcagg tagtaaactt tttcttcctc
    10141 aaggctcttt atacggcgct ttgcggcaag catttctacc cagttgctac tatcaaaaat
    10201 cagcatccag attaaaaaaa taaaaactgt caggaaaaac ttattcctaa gcttgggcaa
    10261 aaaccatttc cagaaagtaa tttcgctaac cggcatgtca acctccacta atcaggtgaa
    10321 taacctgcac atcatcgcca tccttgatgg ttgctaggct atactcgtcc tttttaacca
    10381 gtttaccatt cactttaacc acaagcattt taaaggagaa atgcttaaag gctagcagct
    10441 cgttaacggt aatagcagcc ttattggtat ctatgtgctc cggcaaattg ttaagtgtaa
    10501 tgtccatagc aaaaagtatt tgggcgtgcc ccctgaaggg gtcgggctac tccgggcttc
    10561 gcttcgctcc ggcaataccc gccccctggc gggtatgtgc tgctgcgcac cctccgtatc
    10621 cctcacgcaa atataaccat aacgcacagc aaaatcataa catcacacaa aagatttttg
    10681 tgattaaaag tctgaaaaaa catcgtttta ttctctcctg tcatgctgag ctagccgaag
    10741 catctctttg taccttctct accttaatgt cttttatagt tctttgtcat gctttcccgt
    10801 tgcgatgcaa tgcttccccc ttgctctttg gaaaaatatt gtgtgcatcg ctttacgtgt
    10861 gggagttgtc atagggttac aaacattatg cccctacggg gctggaagcg ttgttcgtgg
    10921 aattgacctc accccagccc ctctcctgtc aggagagggg agggccgtta aagtctcccc
    10981 tctcagggga gatttagagg ggtcatctag gttgaatgtg gaaaacagtc tcaccacagt
    11041 cctttaccct gagaaggaat ggagcggaaa aagcaagcag tggcataaaa gccagacccg
    11101 gtaatgggtc gggggtggaa cgtggcggtg ggcttagtaa gcgataccac atgagtcacg
    11161 ccacaggcgt ggcgaatagt atcgcgtggc agttccaccc gcggggcacc catcgggtcg
    11221 ggcttttcag ccttgatttt ctttggttct ttcttgtatc aagacaagaa agaacatgtg
    11281 aatggctctt tgtcatgctt tcccgttgcg atgcaacgtt acccctttgc tctttggcat
    11341 atgtattgtg tgcatcgcta tactagtgga ggtagcactt tggttacaaa tattacgccc
    11401 ctacagggct gggagcgttg ttcgtggaat tgacctcacc ccagcccct
//
LOCUS       DAOSAL010000077        11434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_3788_length_11434_cov_47.834871, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000077 DAOSAL010000000
VERSION     DAOSAL010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 11434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11434
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3788_length_11434_cov_47.834871"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(346..2727)
                     /locus_tag="PK049_09695"
     CDS             complement(346..2727)
                     /locus_tag="PK049_09695"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOK61870.1"
                     /translation="MKYRLLALITVTFLVSSCQKSLRIEHATTSPAVHDLLSVIQLAG
                     DSTLVYLTDYFPDPQVIDSVSIPSHFQYSISDSKAYLTLKADNQSLPWLSYIFIWAKG
                     DKYCLLVRKNRKLPVTFTFDPQGKKVKSVQLAGQINDWNPSSTNLTFDGKVYKTTKLL
                     NPGKYHYQVVIDGKWMLDPANPTFEDNGMGGYNSVLVVGNTNPEELPKLVPFSTDGNK
                     LFIKAIGNIDKFIVLWQNYLMDDKFITSKEGFYEITIPENARGYKRSHIRIWAYNQQG
                     ESNNLLIPLEGNRVVINPSQLSRSDKHTQIMYFLLVDRFKNGNPSNDFKVNDPEIHPK
                     ANYFGGDLAGVTQKVKDGYFTKLGINTIWLSPITQNPLGAWGLYPQPRTKFSGYHGYW
                     PVSSSKVDFRFGTESELNELIAEAHKRNMNVILDYVANHVHQEHPLYKQHPDWATSLY
                     LPDGSLNTERWDEYRLTTWFDTFLPTLDLERPEVYEPMTDSALFWVSHYDLDGFRHDA
                     TKHIHENFWRRLTQKIKLNLPNKSVYQIGETYGSHELIASYIGSGMLDAQFDFNIYDA
                     SVAAFARNDYPFTKLDGALHQTFSYFGWINLMGYISGNQDRGRFISYAGGALRFEEDA
                     KKAGWTREIGVGDSTAYAKLCMLNAFNMTIPGVPTIYYGDEFGMPGGNDPDNRRMMKF
                     EGLSTLEQQTLETVTKLVNIRRNNLALVYGSFQTILVNDNVYAYVRKYFDNTVVVIFN
                     KSNQEQVIDLQLPEYMSDSVLYPNFNSKYSIQNGKLEVTLKPNSFEIFTTRIN"
     gene            complement(2748..4571)
                     /locus_tag="PK049_09700"
     CDS             complement(2748..4571)
                     /locus_tag="PK049_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HOK61871.1"
                     /translation="MSTMFVVMTSLAQKPVERIEPPCWWVGMNNPNLQLLVYGKNIAS
                     AQVSINNPNIDLVGIDRVENPNYIFINLTVKPDAKPGSIDIKFTFPKGKPLTYAYELK
                     ARQAGSANRKGFDNSDVVYLVYPDRFSNGNPAIDQVSGYTDKVDRNEPYARHGGDIQG
                     ILNHLDYFKQLGVTALWLNPVLESNQPSWSYHGYAITDFYRVDPRLGSNELYKEFIDK
                     AHAQGIKVVKDMVFNHCGGEHWWMKDVPMKNWFNLYPDYKRTNYRIPTTFDPHASVAD
                     SSLMADGWFDGHMPDLNQRNPYLANYLIQNSIWWIEYAGLDGIRMDTHPYCDKHFMAQ
                     WCAAVQNEYPNFSIVGETWVNYPAWVAYWQKDAKNRDGYNSNLEIVMDFPLMFAMHKA
                     FDEKDGWDTGLARLYEIIAHDFVYPNPDKLFIFADNHDIGRFQRDSSQALGRLKLAMA
                     FLLTTRGIPQIYYGTEILLPGDDAKGHGDIRRDFPGGWPTDSKSAFTPEGRTKRQNEI
                     WNYMSTILNWRKTATAIHNGKLTHFIPENEVYVYFRHNSEQTVMVILHNGYQPKVLDT
                     SRFAEFLSNKTSGIDIISGNKVENLAKIQLSPRSAMIIELK"
     gene            complement(4750..5814)
                     /gene="fbaA"
                     /locus_tag="PK049_09705"
     CDS             complement(4750..5814)
                     /gene="fbaA"
                     /locus_tag="PK049_09705"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOK61872.1"
                     /translation="MKRFKPGVITGKELQELFAIAKAEQFALPAVNVTGTNTMNAVLE
                     TARELGSPVIIQFSNSGASFIAGKSLSNDGQQASILGAISGAKHVHLLAEAYGVPVVL
                     HTDHAAKKLLPWIDGLLDAGEKHFRETGKPLFSSHMLDLSEESLHENIEICKRYLERM
                     AKMGMTLEIELGVTGGEEDGVDNTGVDSSRLYTQPEEVAYAYEELMKISPNFTIAASF
                     GNVHGVYKPGNVVLNPIILKNSQELIKQKYATADKPVNFVFHGGSGSEKEKITEAISY
                     GVIKMNIDTDLQWAFWEGIKNYYEAKKGYLQGQIGNPEGEDAPNKKYYDPRVWLRKGE
                     ESIVARLKEAFADLNAIGRG"
     gene            complement(5864..8188)
                     /locus_tag="PK049_09710"
     CDS             complement(5864..8188)
                     /locus_tag="PK049_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HOK61873.1"
                     /translation="MNPYLKHDEWCIIEEGFIPENTRASESIFSIGNGAMGQRANFEE
                     KFTGSTLQGSYIGGVYYPDKTRVGWWKNGYPEYFAKVINSTNWIGINVIVNGEELDLA
                     KCKVQDFRRVLNMKEGYLERSFIAELQNGIKLAVKSTRFLSMVDSELGAIRYSVKPLN
                     ADANIEVHPYLDADVHNEDSNYGEKFWEVVDIKGTSAFSYIVSRTKKLDFHVGHAMRN
                     FIYVNGQHQNVQPQVNTSAMYVDNRFTINAKAGDEVTLYKYAAVISSLNHEKGTFPKV
                     LEKLIDTAVSKGFDKMLDEQRNAWTEIWQHSDITIEGDVAAQQGIRFNIFQLSQTYTG
                     KDERLNIGPKGFTGEKYGGSTYWDTEAYCLPFYMVTHGQKVARQLLLYRYKQLGKAIE
                     NAQKLGFTDGAALYPMVTMNGEECHNEWEITFEEIHRNGAIAFAIYNYVRYTGDKSYL
                     VNFGLEVLIAIARFWAQRVNFSQEKGKYVMLGVTGPNEYENNVNNNWYTSTIACWCME
                     YAEEVASYVKSTDPSKYAELTKSIHFDESKEIAKWRDIRNNMFFPVDEKLGIFLQQEG
                     YMDKEQILVKDLDPKERPLNQKWSWDRILRSCFIKQADVLQGLYFLEDRYDTDTIKRN
                     YEFYEARTVHESSLSPCVHSVLASRIGNVDKAYELYLRTARLDLDDYNREVKEGLHIT
                     SMAGTWISIVEGFGGMRVRNNMLTFNPLIPDKWKSYSFKVWFRDNILKIMVTSSQITI
                     NNESGNAITLKIYGVEHSVPASSSISVPVQKQIF"
     gene            complement(8194..8856)
                     /gene="pgmB"
                     /locus_tag="PK049_09715"
     CDS             complement(8194..8856)
                     /gene="pgmB"
                     /locus_tag="PK049_09715"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0046352 - disaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HOK61874.1"
                     /translation="MNEIKACIFDLDGVVVDTAKYHYLAWKRLAQELGFEFTEQHNER
                     LKGVSRMRSLEILLEVGGLSFPLDEMERMAAKKNAWYVEYIERMTPDEILPGAKELLE
                     ELRANGIKVSLGSASKNSPLILERIGLKNHFDAVIDGNSVTVAKPAPDIFLVAAKSLG
                     VNPEFCVVFEDAEAGIEAAINAGMRTVGIGNPQILNRADVVVPNLKGFTYSKLIELFL
                     KK"
     gene            complement(8861..10189)
                     /locus_tag="PK049_09720"
     CDS             complement(8861..10189)
                     /locus_tag="PK049_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOK61875.1"
                     /translation="MAKKPKLSFWQIWNLSFGFLGVQAGFALQNANVSRILSNYGADL
                     HHLSFFWLVAPIMGLIIQPWVGAASDRTWTRMGRRKPFIFGGAIAASLGMFFMPNSHI
                     AIAILPPVIFGAIMLALMDASFNVTFQPFRSLVADMTPEEQTNTGYSIQTLLINLGAV
                     IGSFLPFVLTNLLGVGNTAPEGQVPPSVVWSFYIGGSILLISVLWTVFTTKEYNPDEF
                     KQYNQSDSVVVEAEKKSFWQTLTSMPGIMGKLAIVQFFSWFALFAMWVYTTPAVAQHI
                     WGTAIGDSTSPEYNEAANWVGVLFGLYSVFAAIFAIFLDKIANRLGRVKTYSISLLFG
                     ALGYLSMYIFTGQYALILSMVGVGIAWAAILAMPYAILARSLPADRMGVYMGIFNITV
                     VVPQIVSGLLLGTILKFVFNEKAIYMIVLAGLSMLLASASAILFVKEKKA"
BASE COUNT         3224 a         2479 c         2500 g         3231 t
ORIGIN      
        1 cagcaggtag agcacatccc ttttaaggat ggggtcctgg gttcgaatcc cagctgaatc
       61 accaaaaagc ccgatggaaa cgtcgggctt ttgtattaag gttaaaggtt aaaggttaaa
      121 ggttaaagat aaaaaggggt aaaagttaaa gagagcctct tccgggaatt tatttgattt
      181 tgcatctaat gtaaatcaaa cctttggcta ttgttgatac aattataata aacacaaaag
      241 aagtatcgta aagcaaaatc ctatacacaa ccttttgctc tccaaaagta aaaaaggtcg
      301 gattattgct aatccgacca ttttattggt tagaaattca ttagcttaat ttattctggt
      361 tgtgaatatt tcgaaactgt ttggcttaag cgtaacttca agtttcccgt tttggattga
      421 gtacttagaa ttgaagtttg gatataatac gctatcactc atatattcag gaagttgtag
      481 gtcgataact tgttcctggt tcgacttgtt gaagattaca actacggtat tgtcaaaata
      541 cttacgaacg taagcgtaaa cattatcgtt tacgaggatg gtttggaatg agccgtaaac
      601 cagagccagg ttatttctgc ggatgtttac aagctttgta acagtttcaa gggtttgttg
      661 ctcaagggtt gataatcctt caaacttcat catgcggcgg ttgtcggggt cgttgccacc
      721 gggcattcca aactcgtcgc cgtagtatat ggttggaacg ccgggaatag tcatgttgaa
      781 tgcgttaagc atgcagagtt tagcataagc agtactatcg ccaacgccaa tttcgcgagt
      841 ccatccagcc tttttggcat cctcttcgaa acgtaaggca ccaccggcat aggaaataaa
      901 acgcccccta tcctgatttc ctgagatata gcccataagg ttaatccagc caaaatagct
      961 gaatgtttgg tgcagcgctc catcaagctt agtaaagggg tagtcgttgc gggcaaaagc
     1021 ggcaaccgag gcatcgtaaa tgttaaaatc gaactgggcg tcgagcatgc cactgccaat
     1081 gtagcttgca atgagttcgt ggctgccata ggtttcgcca atctggtaaa ccgatttatt
     1141 gggtaggtta agtttgattt tttgggtaag cctacgccag aaattttcgt gaatatgctt
     1201 agtggcatcg tggcggaatc catcgaggtc gtaatgggaa acccagaata aagctgagtc
     1261 ggtcatgggt tcataaacct caggacgttc caggtcgagc gttggaagaa acgtatcgaa
     1321 ccaagttgta aggcgatact catcccagcg ttcggtgttt agtgaaccat cgggcaggta
     1381 aagcgatgta gcccagtcgg gatgctgctt gtaaagggga tgctcctggt gaacgtggtt
     1441 ggccacgtaa tcgagtataa cgttcatgtt tcgcttatgg gcttcagcaa taagctcatt
     1501 aagttcactc tcagtcccaa acctaaagtc gaccttggaa gaagaaaccg gccagtagcc
     1561 atggtagccc gaaaatttgg tgcggggttg tggatataat ccccatgccc ctaaggggtt
     1621 ttgagtaatt ggtgaaagcc atatagtatt gattcccagt ttggtaaaat atccatcctt
     1681 aaccttttgg gtaacgccag ccaggtcgcc accaaagtaa tttgccttgg ggtgtatttc
     1741 gggatcgttc accttgaaat cgttggatgg gttgccgttc ttaaagcggt caaccaaaag
     1801 gaaatacatg atttgagtat gcttatcgct acgggaaagc tgcgatggat ttattaccac
     1861 cctatttccc tcaagcggta taagtagatt gttcgattcc ccttgctggt tgtaggccca
     1921 aatgcgtata tgggatcgct tgtaacctcg ggcattttca ggtattgtta tttcgtaaaa
     1981 tccttcctta gaggtaataa atttatcatc cattaagtaa ttctgccaca gcacaataaa
     2041 cttatctata tttcctatcg ctttaataaa caatttattt ccatccgttg agaatggaac
     2101 gagtttgggt agctcctcag gatttgtatt gccaacaact aacactgagt tatacccacc
     2161 cattccatta tcttcaaaag tggggtttgc tgggtcaagc atccacttgc cgtcaataac
     2221 aacctggtaa tggtatttcc ccggatttaa aagcttagta gttttataaa cctttccatc
     2281 gaatgtaaga ttggtagaac tgggattcca gtcgtttatt tgccctgcaa gctgaacgct
     2341 ttttaccttt ttcccctgag gatcgaaggt gaatgttaca ggaagtttac gatttttccg
     2401 aaccaataaa cagtatttat cgcccttagc ccagataaaa atatatgaga gccatggcaa
     2461 tgactgattg tcagctttca aagtaaggta tgccttacta tcggaaatgc tatactgaaa
     2521 atgtgagggt atagatactg aatcaatcac ctgaggatca gggaagtaat cggttaggta
     2581 aaccaatgta ctatcgccag caagctgaat tactgaaagc aaatcatgaa ctgccgggct
     2641 ggtagtagca tgttcaatcc gtaaactttt ttggcatgaa cttactaaaa aggttacggt
     2701 aatcaatgct aataatctgt atttcatata cataatattt taaagattta cttcaactca
     2761 attatcatag ccgagcgggg cgaaagctga atctttgcaa ggttttcaac cttatttccg
     2821 gaaataatat caattccgct cgttttgttg gaaaggaact cagcaaagcg agaggtatcc
     2881 aaaaccttgg gttgataacc gttatgaaga ataaccatca cggtttgctc tgagttatgg
     2941 cggaaataaa catatacctc attctcaggg ataaaatggg ttaattttcc gttgtgaatg
     3001 gcagtagccg ttttgcgcca attcaggatg gtagacatgt agttccagat ttcattttgg
     3061 cgctttgttc taccctcagg ggtaaaagcc gacttggaat cggttggcca tccgccggga
     3121 aaatcgcggc gaatatcgcc atgccccttg gcatcatcgc cgggcaataa aatctcagtt
     3181 ccgtagtaaa tttgaggaat gccacgtgtg gtaagcagga atgccatggc gagctttaaa
     3241 cggcctaatg cctgagaaga atcgcgctgg aaacggccga tatcgtgatt atcggcaaag
     3301 atgaaaagtt tatcgggatt tgggtaaaca aagtcgtggg caataatctc gtaaaggcgt
     3361 gccagtcccg tatcccatcc atccttctca tcaaatgcct tgtgcatggc aaacatcagg
     3421 gggaaatcca ttacaatctc taggttcgag ttatacccat cgcggttttt ggcatccttt
     3481 tgccagtatg ccacccaagc aggatagttt acccaggttt cgccaacaat gctgaagtta
     3541 ggatattcat tctgaaccgc agcgcaccac tgggccatga aatgcttatc acaataagga
     3601 tgggtatcca ttcggatgcc atccaacccg gcatactcaa tccaccagat actgttctgt
     3661 attaggtagt tggcaaggta tgggtttcgc tggttaaggt cgggcatgtg gccgtcgaac
     3721 caaccgtcgg ccattaggct gctatcggca accgaagcat gcgggtcgaa ggtggtagga
     3781 atgcggtagt ttgtccgttt gtaatcgggg taaaggttga accagttctt catgggcaca
     3841 tccttcatcc accagtgctc acccccgcag tggttgaaaa ccatatcctt aactacctta
     3901 atgccctggg cgtgggcttt atcaatgaat tccttgtaaa gctcattgct acccaggcgg
     3961 gggtcaaccc tgtaaaaatc ggtaatggca tagccatggt acgaccacga aggctggttg
     4021 ctctcgagca caggatttaa ccaaagcgct gttactccca gttgtttaaa atagtcgagg
     4081 tgattcagaa taccctgaat atcgccgccg tgacgggcgt aaggttcatt gcggtcaacc
     4141 ttatcagtat atccgctaac ttggtcaatt gcagggtttc cgttggaaaa cctgtcgggg
     4201 taaaccaggt aaaccacatc ggaattatca aagcctttgc ggttggctga gccagcttga
     4261 cgggctttaa gttcgtaagc ataagttagt ggtttaccct taggaaaggt aaacttaata
     4321 tcaatactcc ctggcttggc atcgggttta acggttaggt tgatgaagat atagtttgga
     4381 ttttcaaccc tatcgatacc aacaagatcg atattggggt tgttgataga aacctgtgcc
     4441 gaggcaatat ttttcccgta aaccagaagc tgaaggtttg gattattcat accaacccac
     4501 cagcaagggg gttcgatacg ctcaaccggt ttctgagcaa gtgatgtcat caccacaaac
     4561 atagtagaca tcagtaaaga taagaggttt ttaaacatgg ttattggttt ttttgagtga
     4621 tttttcctga aaacaagaag ggctgtcagg gaaaccccca acagcccaaa ttctaataaa
     4681 taactatttg gatatgaata cttttattac cagctggaca aaaatccgga atatggaata
     4741 agctattcat tacccacgac ctatagcatt aagatcggca aaagcttcct tgagacgggc
     4801 aacaattgac tcttcgcctt tgcgaagcca aacgcgagga tcgtaatact tcttgttggg
     4861 agcatcttcc ccttcgggat tcccaatttg accctgcagg taacccttct tggcctcgta
     4921 atagtttttg atgccttccc agaaagccca ctgcaaatcg gtatcaatat tcatctttat
     4981 tactccatag ctgatggcct cagtaatttt ttccttttcg gaaccgctac caccatggaa
     5041 tacaaagttt acaggcttat cggctgtagc atatttctgc ttaattagct cctgtgagtt
     5101 cttaaggata attgggttaa gaaccacatt gccgggcttg taaacgccat gcacatttcc
     5161 aaacgatgct gctatggtga aattggggct aattttcatc agctcctcat atgcgtaagc
     5221 cacttcctcg ggttgggtgt ataatcttga gctgtcaaca ccagtattat caacgccatc
     5281 ctcctcgccg ccagtaacgc caagctcaat ttccagggtc attcccattt tggccattcg
     5341 ctccaggtaa cgcttgcaga tttcgatatt ttcatgaagc gattcctcgg aaagatcgag
     5401 catgtgtgag ctgaaaagag gttttcctgt ttcgcggaaa tgtttttcgc ccgcatcgag
     5461 caagccatca atccatggaa gaagtttttt ggcagcgtgg tcggtgtgaa gtacaacggg
     5521 aacaccatag gcctcggcca gcaaatgaac atgcttagct ccgcttattg ccccaagtat
     5581 actagcctgt tgaccgtcgt tggatagcga tttgcctgca ataaacgatg ccccgctgtt
     5641 tgaaaactga ataataaccg gtgagcccaa ctcacgggcg gtttccagca cggcattcat
     5701 tgtattagtt ccagtaacgt taactgcagg tagagcaaac tgctcagcct tagcaattgc
     5761 aaatagttcc tgtaactctt ttccggttat tacgcctggt ttaaaacgtt tcatgatttt
     5821 gtagttttaa gaaatacaac gcaaaggtaa tcttttttca ggcttagaaa atttgtttct
     5881 gaaccggaac gcttattgag ctggaggcag gaactgaatg ctcaacaccg taaatcttaa
     5941 gggttattgc attcccgctt tcattgttaa tggttatttg tgaactggtt accattatct
     6001 taagaatgtt atcgcggaac caaaccttaa agctatacga tttccactta tcgggaataa
     6061 gcgggttaaa agtgagcatg ttgtttcgaa cgcgcattcc accaaaacct tcaacaatgg
     6121 aaatccaggt gccggccata ctggtaatgt ggagtccctc cttaacctcg cggttgtaat
     6181 cgtcgagatc gaggcgagct gttcgtaggt aaagctcata agctttatca acattgccta
     6241 tacgagatgc cagaacgcta tgaacgcatg gtgaaagcga tgattcatga actgttcggg
     6301 cctcgtaaaa ctcataatta cgtttaatgg tatcggtatc gtacctgtcc tcaaggaagt
     6361 ataatccctg taaaacatcg gcttgcttaa tgaagcagga acgcagaata cgatcccaac
     6421 tccatttctg atttaacgga cgctccttgg gatcaagatc cttaacaaga atctgctcct
     6481 tgtccatata cccttcctgt tgcaggaata ttcctaactt ttcatcaaca gggaagaaca
     6541 tattgttacg gatatcgcgc cacttggcta tctccttaga ttcatcaaaa tggattgatt
     6601 tagtcaactc agcatacttg gatggatcgg ttgatttaac gtaagatgct acctcctcag
     6661 catactccat gcaccagcaa gcaatggttg aagtgtacca gttgttatta acgttattct
     6721 catactcatt agggccggta acacccagca taacgtactt acccttttcc tgcgagaagt
     6781 tcacacgctg tgcccaaaaa cgagcaattg caatgagcac ttcaaggcca aagtttacca
     6841 agtaactttt atcgcctgta tagcgaacgt aattgtaaat tgcaaaagca atggcaccat
     6901 tacgatgaat ctcctcaaag gtaatttccc actcgttgtg gcactcctca ccgttcatgg
     6961 taaccattgg ataaagggct gcaccatcgg taaagccaag tttttgtgca ttttcaatag
     7021 cctttccaag ttgcttgtag cggtagagca gaagttgacg ggcaaccttt tgaccgtgtg
     7081 taaccatata gaaaggtaaa cagtatgcct cggtatccca ataggttgat ccaccatact
     7141 tttcacctgt aaagcctttt gggccaatgt tcaagcgttc atccttgccc gtataggttt
     7201 ggcttagctg gaaaatattg aagcggatac cctgctgagc agccacatcg ccctcaatgg
     7261 tgatatcgct atgttgccaa atctcagtcc aagcatttcg ctgctcatca agcattttat
     7321 cgaaaccttt tgatacggca gtatcaataa gcttttcgag aaccttaggg aatgttccct
     7381 tttcatggtt caggcttgag attacagcag catacttgta gagtgttact tcatcgccag
     7441 cctttgcatt aatggtaaac ctgttgtcga catacatagc cgaggtgttt acttgcggct
     7501 gaacattctg atgctgaccg ttaacgtaaa taaagttacg catggcatgg ccaacgtgga
     7561 aatcgagctt tttagtacgg cttacaatgt agctgaaagc tgatgtgcct ttaatatcga
     7621 ccacttccca gaacttttcg ccatagttgg aatcctcatt gtgaacatcg gcgtctaggt
     7681 atgggtgaac ctcgatatta gcatcggcat taagcggttt aacagagtaa cgtatagcgc
     7741 caagttcaga atcgaccata cttaaaaatc gtgttgactt aacggccaac tttataccgt
     7801 tctgtagctc ggcaataaat gaacgttcaa ggtatccctc cttcatgttg agtaccctac
     7861 ggaaatcctg aactttacac ttggccaggt cgagctcctc gccgtttaca attacattga
     7921 ttccaatcca gttggtagag ttgataacct tggcgaaata ctcagggtag ccatttttcc
     7981 accaacccac tcgggtttta tcggggtagt aaacgccacc tatgtagctt ccctgaagcg
     8041 tgctgccggt gaatttctct tcaaagttag cacgctgtcc catagcacca tttccaatgc
     8101 tgaaaatact ctccgaagcg cgagtatttt caggaataaa tccctcttca atgatgcacc
     8161 actcgtcgtg tttaagataa gggttcatat cgattacttt tttaagaata gttcaattaa
     8221 tttgctatat gtaaaacctt taagattagg cacaaccaca tcggcacggt tcagaatttg
     8281 aggattgcca ataccaacag tgcgcatacc tgcattaatt gcagcctcaa taccggcttc
     8341 ggcatcctca aatacaacac aaaactcagg atttacccca agcgattttg cagcaaccag
     8401 gaagatatca ggtgccggtt tggctactgt aaccgagtta ccatcaatta cagcatcgaa
     8461 atgatttttt aaaccaatcc gttcaaggat aaggggcgaa tttttgcttg cagaaccaag
     8521 agaaactttt atgccattag cccttaactc ttccagcaat tcctttgcgc caggaagaat
     8581 ctcatcgggg gtcattcgtt caatgtactc cacgtaccag gcatttttct tggcagccat
     8641 tcgttccatt tcatccaatg gaaacgacaa accaccaacc tccagcaaaa tttcaaggga
     8701 gcgcatacgg ctaactcctt taaggcgctc gttatgctgc tcggtaaact caaagcccaa
     8761 ctcctgcgca aggcgcttcc aagccaagta gtgatacttt gcagtatcga ccactacccc
     8821 atctaaatcg aaaatacaag ccttaatctc attcatcggg ttaagccttt ttctctttaa
     8881 caaaaagaat agcgcttgcc gaagctaaaa gcatcgataa accagcaaga actatcatat
     8941 agattgcctt ttcgttgaaa acaaatttga gaatagttcc aagcagtaaa ccacttacaa
     9001 tttgcggtac taccacggtt atgttgaaaa tgcccatata cactcccatt ctatcggctg
     9061 gtagcgaacg cgcaagaatt gcgtaaggca tagccagaat ggcagcccaa gcaattccaa
     9121 cacctaccat tgaaagaata agagcatatt gaccggtaaa tatgtacatc gataaatagc
     9181 ctaatgcacc aaaaagaagc gaaatggaat aggtttttac tctacccaag cgattagcta
     9241 tcttgtcgag gaaaatggca aaaatggccg caaaaacgct atataatcca aaaagaaccc
     9301 ctacccagtt agccgcttcg ttatactcag gagaggtgga atcgccaatg gctgttcccc
     9361 aaatatgctg ggcaacggct ggtgttgtat aaacccacat ggcaaacaga gcaaaccaag
     9421 aaaagaactg aacaatggct agtttgccca ttatacccgg cattgatgtt aaagtttgcc
     9481 agaaactttt tttctcagct tcaacaacaa cgctatcgga ttggttgtac tgtttaaact
     9541 catcagggtt atattccttg gttgtgaaca cagtccacaa aaccgatatc aacagaatag
     9601 aaccaccaat ataaaacgac caaaccacgg aggggggaac ttgtccctca ggagcggtat
     9661 tacccactcc aagcaaatta gtgaggacga aaggtaggaa agaaccaata acggcaccca
     9721 gattgataag cagggtttga attgaataac cagtattggt ttgctcttcg ggggtcatgt
     9781 cggcaaccaa agagcgaaaa ggctggaaag taacgttgaa cgaagcatcc attaatgcca
     9841 gcattattgc tccaaaaatt acaggaggta gaatggctat tgcaatatgt gagttaggca
     9901 taaaaaacat tcctaatgaa gccgcaatag cacctccaaa aataaaaggt ttacggcgcc
     9961 ccattctagt ccatgtccta tcgcttgctg ctcctaccca tggctgaatg attagcccca
    10021 tgattggagc tacaagccag aaaaaagaga ggtgatgaag gtcagcacca tagtttgata
    10081 gtatgcgtga aacattggca ttttgcaggg caaaacctgc ctgaacccct aaaaagccaa
    10141 agctaaggtt ccaaatttgc caaaagctga gtttaggttt ctttgccata aagctatttt
    10201 tagttttcaa ggtgtccaaa attaaagtaa aaaaatctta aaaaaaacat aacataaagt
    10261 taaaaccctt atgaaataag ggtttcagaa catgaaagag gcatttcaat cgtttacgaa
    10321 gggttgaaga aatatttcaa aaattttcat tacggaaacc cttgaacatc aggaaaaccg
    10381 cgtaaaatca accgtaaacg tttgcggtaa atttatcgat gggtgtgttt tgatattgaa
    10441 tttaaattga acgcagatga cgcagattgt gtggatttac tctgtgaaac gattaaagtt
    10501 gatggttgtt agttgattgt tgattgttga tagtttggtg ctgcctcgtc ttatcctgtc
    10561 atgctgagcg caaccgaagc atcactatgt gctatcatcc cttatttaat gagattcctc
    10621 gacaagctcg gaatgacaaa aaacatactt cttctactcc attgtcatgc tgagcaccgc
    10681 cgaagcatct ctttttacct ccaattcatt tatacctttt gattaggttc caagcagtca
    10741 cttttgggaa tgtttttcct cttgcgatgc aacgtttcac ccttgctctt tggaaaatgt
    10801 attgcgtgca tcgctatgca tgagtgagta acaattgtgt tacaaatatt acgcccctac
    10861 ggggctggga gcgttgttcg ttgctcgtga atcgttgttc gtaaatcgtt gttcgtaatt
    10921 ggaatggaat tcagatgacg cagattgtgt ggatttacac tgtgaaacga tttaagttga
    10981 tggttgttgg ttgattgttg atagtttggt gttgcctcgt tctaccgtgt catgctgagc
    11041 ttgccgaagc atctctttgt aacttcacta cctatatttc ttttttaatt tgattttggc
    11101 aatcgctctt tgccatgttt tgctgttgcg atgcaacgtt tcactcttgc tctttggaat
    11161 gccatggcgt gcatcgctat acatttaaga ataatcatgg ggttacaaat attacgcccc
    11221 tacggggctg gaagcgttgt tcgttgctcg tgaatcgttg ttcgtggaat tgacttcacc
    11281 ccagccattt tccctgagag ggaatggagc ggaagaagca tgcagaggca taaaagcctg
    11341 acccggcaat gggtcggggg tggaacgtgg cggtgggcct agtaagcgat accacatgag
    11401 tcacgccaca ggcgtggcga atagtatcgc gtgg
//
LOCUS       DAOSAL010000078        10731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_4063_length_10731_cov_47.527445, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000078 DAOSAL010000000
VERSION     DAOSAL010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 10731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10731
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4063_length_10731_cov_47.527445"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(267..1331)
                     /locus_tag="PK049_09725"
     CDS             complement(267..1331)
                     /locus_tag="PK049_09725"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOK61876.1"
                     /translation="MKRSLQHRFFCLFRLVFLVCLAGLPIIMLAQNQAASRHRVMFYN
                     VENLFDPFDDSLTRDDEFTPTGQRYWTWNKMETKINGIYKVIMAVGELDPPVLVGLCE
                     VENGFVLHKLVNDTPLSKFEYRVVHRESPDPRGIDVALLYRPDRFKVQEREFVPVIFP
                     DDSARKTREILYVSGMLGSDTLHVFVNHWPSKYGGELESESGRFAAANTLKRKIDSIR
                     VFYPQARILVMGDLNDEPESAPVVEGLGACERNDPNCPSGLINIAAILKSRGFGSYKY
                     QGTWGIIDQIIVSQSLLSGKHRLYTTPQKAAVFESDQLLEPDKQYSGNRPFRTFIGYR
                     YHGGFSDHLPVYIDLLENEP"
     gene            complement(1343..2785)
                     /locus_tag="PK049_09730"
     CDS             complement(1343..2785)
                     /locus_tag="PK049_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61877.1"
                     /translation="MFKKVLNRFLVIILSLLALWSCENDNFSSSPSIKLTFSTDTVLF
                     DTVFTTIGSSTRYLKVYNRSKSDVRISSVSLAGGSTSAYRINVDGRPGPLVNDIPLRS
                     GDSLFVMVEVTVNPTLGDAPLLIPDSIVFVTNTNVQDVKLVAWGQDVYMHNGVTIETD
                     SVFPVNKPHLIYDYLFVDKNATLTIPAGARLHFHNNAQLVVAGILKVEGSASLPVTMQ
                     GDRLEKFYSDKGGQWGGVWLSAGSRNNAIAWADIRNAIIGLMVDTCFTPGVPTLVLSH
                     SKIGNSSYVNLLARGAKLEVDNCLFYNAAYSSVALTLGGTYRFYHTTISNYWGDYMFR
                     KGPALLLNNYYTYQLVPGGPVLVEPRDLNEASFFNSIIYGSISNELGLDYKYNGQPVN
                     ANFNYSFSDCILRVPSTYNLNDPEHFTRVIREDPRFKDIAKWNFELDTLSPAKDIGYI
                     TIATGFPVDLKGVNRLADGKPDLGAFERVE"
     gene            2894..4486
                     /locus_tag="PK049_09735"
     CDS             2894..4486
                     /locus_tag="PK049_09735"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOK61878.1"
                     /translation="MACTKRQKADLIVTNATVYTVDSAFTVAQSFVVSNGKIVAVGTN
                     NEILSAYSADTILDLTDKFVYPGFIDAHCHFLGYGLNLPKAWLAGAKSWNEVVNRLVE
                     HQNKYPTEWVEGRGWNQNEWEIKEFPTNELLNKAFPNKPVFIVRIDGHAAIANRKALE
                     LAGVTASTKISGGEIVVKNGIPTGVLIDNAMELIHKHIPTPSKEQKAQALLNAQQNCF
                     AVGLTGVHDAGLDADEVELIDSLQQAGNLKMRINAMLNPTHDNFEKYISKGIYSTAQL
                     TVRSIKIYADGALGSRGALLLKPYTDDPKTRGIQVEPTEKLDSVCSLAYKHGYQVCTH
                     CIGDAAVRLMLDTYSKYLPQGNDLRWRIEHSQVVDPADLPRYGEFDVIPSVQTTHATS
                     DMLWAVNRLGDRVKYAYAYQNLLAQNGWLPNGSDFPIEDINPLYGFYAAVARKNLNGE
                     PQEGFQMENALTREQALRAMTIWAAKTCFEEEVKGSIEAGKLADFVVLDLDLINSEIS
                     NALKAQVLQTFINGENVYTKNQ"
     gene            4483..4953
                     /locus_tag="PK049_09740"
     CDS             4483..4953
                     /locus_tag="PK049_09740"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOK61879.1"
                     /translation="MTIEIVKADKTNLIEVLYIIRECSRQLIEKGVKYWNNSLADRFE
                     ISTDIESGFIYLIKYNYVPVGTITLRFLPKKESLQLERLAIFPAYQKKGLGQKLIEFA
                     KETAKEKGVKKILGSIPIDDADLIHLLERNNFVKKGESIPVEMNTIALELELLQ"
     gene            5172..8405
                     /locus_tag="PK049_09745"
     CDS             5172..8405
                     /locus_tag="PK049_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOK61880.1"
                     /translation="MHKIDKHPILEVPQRETVTFMFNGNEVKGEKGFTIAAALHQAGF
                     PVHSHSIENRERSLECGIGKCGACEMLVDGQIKRICITPVDGVKEVKEIPHEYTPDVK
                     PANKKESTKVYKTTVTIIGAGPAGLAAREILLEHGVENIVIDNNSTIGGQFNMQTHQF
                     FFFEREKKFGGMRGFEIASTLAGANHEGIFLNSTVWDILENKRIAIKNIKTEEIYYVE
                     SDYLVIATGAVPFMPTFENDDLPGVYTAAVVQKMMNQEFTLLGKNVLTVGAGNIGYLT
                     SYQLMQAGARVKAIIEAMPREGGFPVQANRVRRLGIPIMLGKILVKAIPNENHTGIIG
                     AVIADAKDFKPIPGTEQIIEGIDAINICTGLVPDDQLLIKGNEVFGRNCYGAGDAIRI
                     GEGTSAVLRGKQVAYEILQDAGIRCSYDTYLNISKEYIDSQQHPVRIIEEPFRPSEER
                     RNRKPFVQIDCLYGFACNPCTFACPHGAITKNSTSTVPHIDFEKCVGCMDCVYQCPGL
                     AIFGYSYAKDWLFLPIEYEAQEGAEVYLVDNNGNILGDGVIEKILRKPNKTNVARVKS
                     LNLHGDDMLAARGFIVKSNYPEPIQIKPYEYKPQEKIYVCHCDDVSLDEILETIGDRK
                     FISADEIKHTTRLGMGACRGKRCIKRLKTTLAPMGIQIVGDATPRGPLSNQVAMGELY
                     PKDVPETYITGINGKKARIEKVSALIAGGGIGGSALFRYMSEAGLRPVMLNYGRGASW
                     RNIAGGRTAFSLPEIADIARQNHEIFKELHKVSDIDYRPINYVTFAHDDNMLKALEAS
                     MAWSDAYMVQPSDFRKEISPFMNDMLSTYKAALITRGCWQATPGKVVDLLRKIGIEHG
                     GTVKEDTELLSVHRNGNGFVALVRDHNKEYIEYHADNFVNALGPEGEKFSRQLGIFTG
                     IYPVKHQAFITRRLPWLGINGNPLGMLIDRRNYKGFVAVYGQQLAETGQIIGCASPAV
                     DPREADKNLKINSQDFLEIASEIFSNWIPQLSSVGFQAVWAGYYVEPRMFIDTKHGLF
                     TGLRGQGFMLGMGLAKMYVDELMGRQVPEYFKRLSLEGDGLPEKAFK"
     gene            complement(8467..9138)
                     /locus_tag="PK049_09750"
     CDS             complement(8467..9138)
                     /locus_tag="PK049_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61881.1"
                     /translation="MRYIVLSFLALVGCTRQSDTERLSRSIDTLSSEIAKLRNELNSR
                     DSLGRKKVFPPNDTVTKATVRVKPKPPKIIKKDTLRVKQKEKPQIADTLLYHFAGGVI
                     SVKVYPWVDSKQLIEVYSLKGGVVITLENINLSYNVSNTLHFRSDGSLERVNEHFNPG
                     ASMYWYDTVIHFEPDNEPTWKITYQYPQRTVEDSMGEKYLWDRNQKRWVKQEIVIETI
                     QPSAP"
     gene            9312..10688
                     /locus_tag="PK049_09755"
     CDS             9312..10688
                     /locus_tag="PK049_09755"
                     /inference="COORDINATES: protein motif:HMM:NF013395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HOK61882.1"
                     /translation="MKQKIQILKFITATFLLAIVLGFTACEKDIPVNPKDKTLNVETA
                     MPQDTFDMATGYLLENFETGTKTAYAGATVTLSTGKWYLNNALIGTSTSDRKNGTKSV
                     RIVNTGKVTMQFNKANGAATVEVYHAKYGTDGNSTWELWVSTNSGSTWSKVGSTVTTS
                     STTLTKATFTVNLSGNVRFEIRKVSGGSYRINIDDFKVYDYSTSPTRDDNMALGNPSG
                     AVASTAYPNNYLMVKPQYVLSYNNSKLTCNWVSWHLSSAWIGSAPRQDDFRADNTLPS
                     SWVWVGGTDYSGSGFDRGHMCPSADRTGSITDNSATFLMTNMVPQAPKNNQITWANLE
                     NYCRTLVDAGNELYIISGPWGQGGTGSAGYKTTISTKKITVPAYVWKVIVVLPVGSND
                     ISRITTSTRVIAVWMPNTQTVNSQPWGYYRTTVDYIESQTGYNFLSNVSTSIQSVIES
                     KVDSGPTN"
BASE COUNT         3182 a         2490 c         2423 g         2636 t
ORIGIN      
        1 ttagcattcc tttttggatg aggaatccta ttaatgaaga gttgaagaaa caaagggatg
       61 cttcggcgag ctcagcatga caatgggagt aaggaaaaag ggggattgtc attccgagca
      121 tatcgaggaa tctcttttgc caggaaaagt ttgaggaaaa gtgaaaagtg aacaattaaa
      181 cccaagtaca atgtattaac agattttttc tattgattta tctaggttaa aaaagagagg
      241 aagcacgcag cactggcgtg cttttcttat ggctcattct cgagaaggtc gatataaacc
      301 gggaggtgat cgctgaagcc gccatggtat cggtacccaa tgaatgttcg gaaaggccta
      361 ttaccactgt actgcttatc gggctcaagc agctgatcgc tttcaaaaac cgctgccttt
      421 tgcggcgtgg tgtataacct atgttttccg cttaaaaggc tttgcgacac aataatttga
      481 tcaataattc cccatgtacc ctgatactta taacttccaa aaccacggct tttaagaata
      541 gcagcaatgt taattaagcc cgatgggcag ttggggtcgt tccgttcaca tgctcccaag
      601 ccttcaacca ccggggcgct ctctggctca tcgttcaggt cgcccattac tagtattcgg
      661 gcttgagggt aaaaaacccg tattgagtcg atcttgcgct tgagagtatt tgcagcggca
      721 aacctgccac tttccgattc cagctcgcca ccatacttgg atggccaatg gtttacaaaa
      781 acatgtagag tatcgcttcc cagcataccg cttacataaa gaatctcacg cgtcttacgc
      841 gccgaatcat caggaaatat aactgggaca aactcacgct cctgaacctt aaacctatcg
      901 gggcggtaaa gcagggccac gtcgattccg cgagggtcag gcgactcacg gtgaaccact
      961 ctgtactcaa acttgctgag tggggtatcg tttacaagtt tgtggagcac aaaaccattt
     1021 tcaacctcgc aaaggccaac cagcacagga ggatcgagtt caccaacggc cattatcacc
     1081 ttgtaaatac cattgatttt tgtctccatc ttgttccatg tccaatagcg ctggccagtg
     1141 ggggtgaact catcgtcgcg ggttagcgag tcgtcgaatg ggtcaaaaag attctcgaca
     1201 ttatagaaca tcaccctatg ccttgatgcg gcctggtttt gagcaagcat tataatgggc
     1261 aagcctgcca aacagactaa aaaaacaagc ctaaacaggc aaaaaaatcg gtgttgtagg
     1321 ctgcgtttca ttctccagag atttactcaa cacgttcaaa ggctcccaaa tcgggtttac
     1381 catcggcaag gcggttaaca ccctttaagt cgactggaaa accagtggca atagtaatgt
     1441 aacctatgtc ctttgctggg ctaagggtat cgagctcaaa attccacttg gcaatatcct
     1501 tgaaccgggg gtcttcgcga ataacccggg taaaatgctc agggtcattc aggttgtagg
     1561 ttgaaggcac cctaaggata cagtccgaaa aagagtaatt gaaattagca ttaaccggtt
     1621 ggccattata cttataatcc agtcctaact cattggatat gctaccataa ataatagaat
     1681 taaagaatga tgcctcgttc aaatcgcgag gttcaaccag aaccggtcca ccaggaacaa
     1741 gttggtatgt atagtagttg ttgagcagga gcgcaggccc tttccggaac atgtagtcgc
     1801 cccagtagtt agatattgtg gtatggtaaa accggtaagt ccccccaagg gtaagagcca
     1861 cacttgagta ggcagcattg taaaaaaggc agttgtcaac ctccagcttt gccccacggg
     1921 caagcaggtt aacgtaactg gaattgccta ttttggagtg cgagagcaca agggttggca
     1981 cacccggtgt aaagcaggta tcaaccatca acccaataat ggcattccta atatcagccc
     2041 aagcaatagc gttattccgg gagcctgcac tcaaccacac cccaccccat tgtccgccct
     2101 tgtcggagta aaacttttcc agtctatcgc cctgcatggt tactggcaag ctagccgaac
     2161 cttcaacctt taggattccc gctactacaa gctgcgcgtt gttatggaag tgtaatcgtg
     2221 ctccagcagg aatggtaagg gtagcattct tatccacaaa aaggtaatcg taaatcagat
     2281 ggggtttatt aacgggaaaa accgaatcgg tttcaatggt taccccgtta tgcatgtaaa
     2341 catcctgccc ccaggcaacc agcttaacat cctggacgtt agtattggta acaaaaacaa
     2401 tggaatcagg tattagcaag ggtgcatcgc caagggtagg gttaacggtt acctcaacca
     2461 taacaaaaag gctatcgccg gaacgtaaag ggatatcgtt caccaatggg cctgggcgtc
     2521 cgtcaacatt tattctgtat gccgacgtgc taccaccagc caacgatact gacgaaatcc
     2581 gcacatcgct tttactcctg ttgtaaacct ttaggtaacg ggttgatgaa ccaatggtgg
     2641 taaacacggt atcgaacagc accgtatcgg tgctaaaggt tagcttgatt gatgggctgg
     2701 acgaaaagtt atcgttttcg cacgaccaca gcgccaacaa tgacagaatg ataactaaaa
     2761 accgatttaa taccttttta aacatcaaca aaaaaataat tttctacttt tggtaaaaac
     2821 tcacaacaaa gatagcttaa cggaaatgaa gacaagctca ttatcaatat ttttaacttt
     2881 aagtatagct gttatggcat gcactaaacg gcaaaaagcc gatttaattg ttaccaatgc
     2941 aacggtttac acggttgact cagcattcac agtagctcaa agctttgtcg ttagcaatgg
     3001 caaaatagta gccgtgggaa caaacaatga gatactctca gcttactctg ccgacaccat
     3061 attggatcta acagataaat ttgtataccc cggatttatt gatgcccact gtcacttttt
     3121 agggtacggg ctaaacctac ccaaggcatg gcttgcgggg gcaaaatcgt ggaacgaggt
     3181 ggttaaccgt ctggttgagc accaaaacaa atacccaacg gaatgggttg aaggcagggg
     3241 atggaaccag aacgaatggg aaatcaaaga gtttccaacc aacgagttac tcaacaaagc
     3301 atttccaaac aaaccagttt ttatagttag gattgatggc catgccgcca ttgccaacag
     3361 gaaagcgctt gaattagctg gcgtaaccgc cagcacaaaa atatcgggtg gcgaaatagt
     3421 agttaaaaat ggcataccca ccggggttct cattgataat gccatggaac tgatacacaa
     3481 gcacataccc acaccaagta aggaacaaaa agcgcaagct ctgctaaatg ctcagcaaaa
     3541 ctgttttgcc gttgggctaa ccggcgttca cgatgccggc ctcgatgccg atgaggttga
     3601 acttatcgac tcgttgcagc aagcaggaaa ccttaaaatg cgcattaatg ccatgcttaa
     3661 ccctacccat gacaattttg aaaagtacat cagcaagggt atatatagca cagcgcaatt
     3721 gacggttagg tcgataaaaa tttatgccga tggcgcattg ggttcacgtg gcgcactgct
     3781 actcaaaccc tacaccgacg atcctaaaac ccgcggcatt caggtggaac caactgaaaa
     3841 acttgattcg gtttgcagct tggcatacaa gcatggctac caggtatgca cgcactgcat
     3901 tggcgatgca gccgttaggc tgatgcttga tacttatagc aaatacctac cccaaggcaa
     3961 tgacctacgc tggcggattg agcactcgca ggtggttgat ccggccgatc tgccgcgtta
     4021 tggcgagttt gatgtaatcc catcggttca aaccacccat gccacatcgg acatgttgtg
     4081 ggcggtaaac cggctgggcg atagggtaaa atatgcctac gcctaccaaa accttctggc
     4141 gcaaaacggc tggctgccca acggcagcga tttcccgatt gaggacatca acccgctata
     4201 tggtttttac gctgccgtag cgcgcaaaaa cctgaatggt gaaccccagg agggattcca
     4261 gatggagaac gctttaaccc gcgagcaagc cctccgagcc atgaccattt gggctgctaa
     4321 gacctgcttt gaagaagagg ttaagggtag cattgaagct gggaagcttg ctgattttgt
     4381 agtacttgac cttgatttaa ttaattccga aattagtaat gcccttaagg cacaagtttt
     4441 gcagacgttt atcaacggcg agaatgtata tacgaaaaac caatgacgat tgaaattgta
     4501 aaagctgaca aaaccaacct aattgaggta ctttacatta tacgggagtg ttccaggcag
     4561 ctaatagaaa agggagttaa gtactggaac aacagcctgg ccgacagatt tgaaatatca
     4621 acagacattg aatcgggctt tatctacctt ataaagtaca actatgtacc tgtaggcaca
     4681 ataaccttaa ggtttttacc taaaaaagag agtttacagc ttgaaaggct tgccatattc
     4741 ccagcatacc aaaagaaagg ccttggtcag aagctcatag agtttgcaaa ggaaactgcc
     4801 aaggaaaagg gagtaaaaaa gatacttggc tcaattccca ttgacgatgc cgacctgatc
     4861 cacctgctag aacggaataa ttttgtgaaa aaaggcgaaa gtatcccagt tgaaatgaat
     4921 acaattgcac ttgaacttga actattgcaa tagttcaata aagggaaatt cctccaacac
     4981 ctcaattaaa aatccttaga ttttccatta gctaaacgca tcagttcctc acattttgat
     5041 atcaaaaaat agcaaccatt aggttgctat tttttattcc ctgacattag tcattgtatt
     5101 acacacgctt ggaaaatact ttcgcaaaaa tttccaaaac aatccaagta acaataaaag
     5161 cattttaagc catgcataaa atagataaac acccaatcct tgaggtacct cagcgcgaaa
     5221 ccgttacatt catgtttaat ggtaatgagg ttaaaggaga aaaaggattc accattgccg
     5281 cagcgctaca ccaggctggc tttccggttc acagccatag tatcgaaaac cgtgaacgaa
     5341 gcctcgaatg cggaattggc aagtgcggcg cttgcgagat gcttgtcgat ggacaaatta
     5401 agcgtatatg tattactcca gtcgatgggg taaaggaagt taaggaaatt cctcacgagt
     5461 acacccccga tgtaaaacca gcaaataaaa aggaaagtac aaaggtttac aaaaccactg
     5521 ttacaatcat tggtgcaggc ccggcaggtc ttgcagcccg tgaaatactt cttgaacatg
     5581 gtgttgaaaa catagtaatt gacaacaact caaccattgg tggtcagttc aacatgcaaa
     5641 cccaccagtt cttcttcttt gaacgtgaaa agaagtttgg cggcatgcga ggctttgaaa
     5701 ttgcaagtac gcttgcagga gctaaccacg aaggtatttt cctgaacagt accgtttggg
     5761 atatacttga aaacaaacgc attgccatta aaaacattaa aaccgaggag atttactacg
     5821 ttgaatccga ttacctggta attgctacgg gagccgttcc gtttatgcca acctttgaga
     5881 acgacgacct acccggtgtt tacaccgctg cagtggtgca aaaaatgatg aaccaggaat
     5941 ttacgctact gggcaagaat gtgctcactg ttggagccgg taacattggc tacctcacca
     6001 gctatcagct aatgcaggca ggtgctcgtg taaaagccat tatcgaagct atgcctcgtg
     6061 agggcggatt ccctgttcag gcaaataggg ttagacgatt aggaatccct attatgttag
     6121 gcaaaatatt ggttaaggcg attcccaacg agaaccatac cggaattatt ggggctgtta
     6181 ttgccgacgc caaggacttt aaacccattc caggtactga gcaaatcatt gagggtatcg
     6241 atgccataaa tatatgcacc ggcttagttc ctgacgatca gctcctgatt aaaggtaacg
     6301 aggtgttcgg gcgtaattgc tacggagccg gcgatgccat tcgcattggc gaaggaacca
     6361 gtgccgtatt aagaggaaag caagtggcct acgaaatctt acaggatgct ggcatacgtt
     6421 gcagctacga cacatacctt aatatcagca aggaatacat cgatagccag cagcatccgg
     6481 tacggataat tgaggaaccc ttccgcccct cggaggaacg acgcaaccgc aaaccctttg
     6541 ttcaaatcga ttgcctatat ggctttgcct gcaacccatg tacctttgct tgtccacatg
     6601 gagcaataac caagaactca accagcaccg taccccacat cgactttgaa aaatgcgtgg
     6661 gatgtatgga ctgcgtttac caatgtccgg gcttagccat tttcggatac agctacgcca
     6721 aggattggct tttcctcccc attgagtatg aagcccagga gggcgctgag gtttacctcg
     6781 ttgataataa cggtaatatc cttggcgatg gggttattga aaaaattctc cgaaaaccca
     6841 ataaaaccaa tgtggcaagg gttaaatcat tgaacctgca tggcgatgat atgcttgcag
     6901 ctcgcggttt tattgtaaaa tccaactacc ctgagcctat ccaaataaaa ccatacgagt
     6961 acaaaccaca ggaaaaaatt tatgtgtgcc actgcgacga tgtaagtctc gatgagatac
     7021 ttgaaactat aggcgaccgc aagtttattt cggccgatga aataaagcac accacacgac
     7081 tgggtatggg ggcatgccgt ggcaaacgct gtattaagcg tctgaaaacc acacttgccc
     7141 caatgggcat tcaaatagtt ggcgatgcca ctccccgtgg tccacttagc aaccaggtag
     7201 ccatgggcga actttaccca aaggatgttc ctgaaacgta cattactgga atcaacggca
     7261 aaaaagcaag gatcgaaaag gtatcggcgc tcatagctgg cggtggaatt ggcggtagtg
     7321 cactgttccg ttacatgtcc gaagccggat tacgacccgt tatgctaaat tacggtcgtg
     7381 gagcaagctg gcgtaatatt gccggtggtc gcacagcctt ctcgctacca gagattgccg
     7441 acattgcccg tcaaaaccac gaaattttca aggaactcca taaggtttcg gatatcgact
     7501 accgccccat caactatgta acttttgctc acgacgataa catgctaaag gcactggagg
     7561 catcaatggc gtggagcgat gcatacatgg ttcagccttc cgatttccgc aaggaaatat
     7621 cacccttcat gaacgatatg ctaagcacct acaaagcagc actcataacc cgtgggtgtt
     7681 ggcaagccac accgggtaag gttgtcgacc ttctccgtaa aataggcatt gagcatggtg
     7741 gtacggttaa ggaggatacc gaactgctaa gcgttcaccg caacggaaac ggatttgtcg
     7801 ccctagtgcg ggatcacaat aaagagtaca ttgagtatca tgccgataac tttgtgaatg
     7861 cccttggccc cgagggcgaa aagtttagcc gtcagctggg gatattcaca ggcatttacc
     7921 cggttaagca tcaggctttc attacccgtc gtttgccatg gctgggcata aacggtaatc
     7981 cgcttggaat gcttatcgat aggaggaact acaaaggctt tgtggccgtt tacgggcagc
     8041 aacttgctga aacaggacaa attattggct gtgcttctcc ggcagttgat ccacgcgaag
     8101 ccgataaaaa cctcaagatt aactcgcagg acttcctcga gattgcctcg gagatattct
     8161 ccaattggat accgcaactt agttccgttg gcttccaagc cgtttgggca ggttactatg
     8221 tggaaccccg catgtttatc gacaccaagc acggtctgtt cactggctta cgaggacagg
     8281 gctttatgct gggtatgggg ttggccaaga tgtacgttga tgagttaatg ggtcgtcagg
     8341 tacccgagta cttcaaacgc ttaagccttg aaggcgatgg cttacccgaa aaggcattca
     8401 agtagaaacc agtaaaaatc gatatgtttg ggactccccg tttggcttat gccaacgggg
     8461 atttttttaa ggagctgatg gttgaattgt ttcaattaca atctcctgtt tcacccaacg
     8521 cttctggttt cgatcccata aatacttttc gcccatggag tcttcaacag tcctctgtgg
     8581 gtattggtag gttattttcc aggtaggttc attatcgggt tcaaagtgga tcacggtatc
     8641 gtaccagtac atgctggcac ccggattgaa atgctcattt accctctcca acgatccatc
     8701 ggaacggaaa tgaagagtgt ttgacacatt gtaactcagg ttgatgtttt caagcgtaat
     8761 aaccacccca cccttcaggc tgtaaacctc aattagctgc ttactatcca cccaaggata
     8821 caccttaacg gaaatgactc ctcctgcaaa gtggtataac aatgtgtcag caatttgagg
     8881 tttttccttt tgcttaaccc gtaaagtatc ctttttgatg atttttggag gtttgggctt
     8941 aacccttaca gtcgctttag taactgtatc gttaggcggg aaaaccttct ttctccctag
     9001 tgaatcccta ctgttcaatt cattccgcag tttggctatt tcgctcgata aggtatcgat
     9061 tgagcgggac aacctttcgg tatcgctctg ccttgtgcat ccaaccaatg ccaaaaacga
     9121 aagtacaata tatctcatgg caacaatttc tttcaataaa acaaaccatg tgccaaatac
     9181 ggatttcata ggggctttaa cagccaaatg cgcctggtga taaaatgtta ataaaatggt
     9241 aacgcgcaac ttgccaatca ttaacatatc tgccttacat ttactgcacc aaccccctaa
     9301 aacctaaagc catgaaacaa aaaatccaaa tcttaaagtt tataacggca acgtttctgc
     9361 tagccattgt attaggattt acagcatgtg aaaaggatat tcccgttaat ccaaaggaca
     9421 aaacattgaa tgtggaaacc gccatgccac aggacacctt tgacatggca acaggatatt
     9481 tactggagaa ctttgaaacc ggaacaaaaa cagcttatgc cggagcaaca gtaacactat
     9541 cgaccggtaa atggtacctg aacaatgccc taattggtac atcaaccagc gataggaaaa
     9601 atggcaccaa atcggtacga attgtcaaca cgggtaaggt taccatgcag ttcaataagg
     9661 ccaatggtgc agcaaccgtt gaggtttacc atgccaaata tggaaccgat ggaaatagca
     9721 catgggaatt atgggtgtca accaacagcg gaagcacatg gagcaaagtg ggttcaacgg
     9781 taaccacctc atcaaccacg cttaccaagg caacctttac ggttaacctt tcaggcaatg
     9841 ttcggtttga gatcagaaag gtttccggag ggtcgtaccg gattaacatt gatgacttca
     9901 aggtttacga ttactcaacc tcacctaccc gcgacgataa tatggcgttg ggcaacccat
     9961 cgggggctgt ggcaagcact gcatatccca ataactacct gatggttaaa ccccagtatg
    10021 tgctatcgta caacaacagc aagcttacct gcaactgggt ttcgtggcat ttgagtagtg
    10081 catggattgg ctctgcccca aggcaggacg actttagggc cgacaacacc ctaccctcaa
    10141 gttgggtttg ggttggcgga accgactaca gcggttcagg gtttgaccgc ggccacatgt
    10201 gccccagcgc cgacagaaca ggctcaatta ccgataactc ggccactttc ctaatgacca
    10261 acatggtacc ccaagctccc aaaaacaacc agattacctg ggctaacctt gagaattact
    10321 gcagaactct tgttgatgca ggcaacgagc tatatatcat ttctggtcca tgggggcagg
    10381 gcggaaccgg ttcagcaggg tataaaacca ccatatccac caaaaaaatt accgttcctg
    10441 catacgtttg gaaagtaatt gtagtgctac cggttggctc aaatgatatt tcgcgaatca
    10501 ctacctcaac acgagtaatt gccgtctgga tgcctaatac ccaaaccgtt aacagccaac
    10561 cctggggcta ctaccgaacc acggttgatt acatcgaaag ccaaaccggc tataattttc
    10621 tatcgaacgt atcaacatca atccaaagtg taattgaatc aaaggttgat agcggaccaa
    10681 caaactaatc ctaaaaaaat caccataaaa aaagggtcgc aattgcttgc g
//
LOCUS       DAOSAL010000079        10462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_4210_length_10462_cov_44.188527, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000079 DAOSAL010000000
VERSION     DAOSAL010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 10462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10462
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4210_length_10462_cov_44.188527"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(380..652)
                     /locus_tag="PK049_09760"
     CDS             complement(380..652)
                     /locus_tag="PK049_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61883.1"
                     /translation="MAKLTKEELEKRLKKYAKSMGFELENQRFYQYLRLNIDFDSFFI
                     LNFLNKEEVIEIIDDKKAINELSILLSDIVDEKLTSTPPYPPLSKN"
     gene            complement(882..1559)
                     /locus_tag="PK049_09765"
     CDS             complement(882..1559)
                     /locus_tag="PK049_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HOK61884.1"
                     /translation="MGIYLIMMIFFVLSFMVSMQLRSKFARYSRMALPRGLSGKEVAE
                     LMLHNHGIYDVKVISVDGELTDHYNPANKTVNLSPDVYHGRSIAAAAVAAHECGHAIQ
                     HAQSYAFLQMRSALVPVVSVASRWVQWVLLAGVILLQTMPSILLIGVIMLGLTTLFSI
                     ITLPVEMDASRRALAWLNSSGLTYGEGALYARDALKWAALTYVVAALGSLATLLYYIG
                     LLNSNDE"
     gene            complement(1613..2374)
                     /locus_tag="PK049_09770"
     CDS             complement(1613..2374)
                     /locus_tag="PK049_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOK61885.1"
                     /translation="MMRIALITGATSGIGRATALRLAMDEFNLIITGRRLERLEELKK
                     EIEVKYKKDVHILNFDIRNLDEVNSAIDSLPERWRKIDVLVNNAGLAVGLNHIQDGVI
                     DDWERMIDTNIKGVLYITRKVAPMMIERGKGHIVNIGSIAGTQVYENGNVYCATKHAM
                     HALSQGMRQDMLKHNIKVTEIRPGLLETEFSIVRFKGDSEKAKNTYVGLTPLFAEDIA
                     DAIHFVLTRPPHVCINDLEVTPVAQANAYYVNRNV"
     gene            2591..4819
                     /gene="purL"
                     /locus_tag="PK049_09775"
     CDS             2591..4819
                     /gene="purL"
                     /locus_tag="PK049_09775"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF002290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HOK61886.1"
                     /translation="MPNRTVTVETARELGLLPEEFEMIKNYLGRTPNYTELSIYSVMW
                     SEHASYKNSIKWLKTLPRKGSAILAEAGTENAGLVDIGNGLACAFKIESHNHPCAVEP
                     YQGAATGVGGINRDIFTMGARPVAQLNSLRFGNLKLDRTRWLIKGVVKGIGDYGNAFG
                     IPVLAGEVFFDDSFNMNPLVNAMSVGLVEKDKVVSAVSKGKGNPVYIVGSATGKDGIH
                     GATFASADITEDSAEDIPSIQVGDPFQEKILLEATQEVIRTGALVGMQDMGAAGIICS
                     TSEMSEKGGHGMRIDLDKVPLRQKNMEAWEILLSESQERMLMVVQKGREADVEAVLNK
                     WDISYAIIGEVTEGDTLEFYYHGELVAKVPASSLVLGGGAPVYDREYREPVYYKEYKK
                     FDINKVPEPADLIDVARFLVTHPNIASKRWVYEQYDTMIGTGNMTTNFPSDAGVYNLK
                     GTNKALVMTVDCNSRYVKADPMVGTMIAVSEAARNISCTGAKPLAVTNCLNFGNPYNP
                     EVYWQFVQAVKGMGTACEKFDTPVTGGNVSFYNQASIGGKEEAVFPTPTIGMIGLLEN
                     KNHHTTLAFKEKGHMIYLIGKSVNDIASSEYLYSYHGVKQSPAPYFNLEEEYEVQRAV
                     RELIARNLIQSAHDISDGGLFIALLESAMPRNLGFDITTDAEIRTDAFLFGEAQGRIV
                     VSVSPSRETQFIDFMVASKVPFSTLGHVTKSEIRVDDVSFGFVADLKKIYDSSLEQLI
                     NG"
     gene            4812..5543
                     /gene="ubiE"
                     /locus_tag="PK049_09780"
     CDS             4812..5543
                     /gene="ubiE"
                     /locus_tag="PK049_09780"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HOK61887.1"
                     /translation="MGKSPNTVVDKRTSSVAAMFNSIAPSYDFLNHLLSLGIDKLWRR
                     RLVKRLLRNNPGEVLDVATGTADLALVMAKKSPTVKIVGIDISESMLAIGMVKVKKWK
                     FLERITLKKASALCIPSPDNHFDAAMVAFGVRNFEDLAQGLMEICRVLKPGGELGVLE
                     FSMPRHWPFNALYRFYFLKFLPWVGGKVSGNRQAYTYLPESVLSFPQGEAFVQILKLA
                     GFNDVEIRVQTFGIATLYIAKKKNQ"
     gene            5599..6333
                     /locus_tag="PK049_09785"
     CDS             5599..6333
                     /locus_tag="PK049_09785"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOK61888.1"
                     /translation="MKRFFALLLLCFLVVGNSFGQFKRPTLLNDPDYDVGKPLRFGFS
                     IGVNVMDFDAVNRTAQFNADGSKYFAEVTHISAGLNVNAIGDLRIARDIHLRFLPGYS
                     FGQRDVNFFKVDADSTVSLATTMKMESNFIDLPVGIKFLSERNSNVRPYLYLGTDVRI
                     DLAAYKRLKVEKGVLLRLQKFDYYYEVGFGVDFFLQYFKFSAEIKWSAGLLNMLSSDY
                     VDEGKIYHDALGSLRSKFLIVSFHFE"
     gene            6390..7946
                     /locus_tag="PK049_09790"
     CDS             6390..7946
                     /locus_tag="PK049_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOK61889.1"
                     /translation="MPHDISIVLRPDEAANETAVVRAIARKIDIPTDSIRHYYITRKS
                     IDARKGKVLVNLCVRVFIGEEKIIDERPIFNYNDVRNAMPVIIVGAGPAGLFAALRLI
                     ELGFKPIVIERGKSVSHRKRDIAMLNREHQLNPDSNYAFGEGGAGTFSDGKLYTRSKK
                     RGDYRRILQALVDHGADPAILYEAHPHIGTNRLPGIITRIRETILQHGGEVLFSSRVT
                     EIIIDSGQAVGVKLADGQRINGKAVILATGHSARDIYHMLHNQSIALEAKPFAMGVRV
                     EHPQALIDAIQYHGIPRSEYLPAAAYSLVHQVEGRGVYSFCMCPGGFIVPAATGTNEV
                     VVNGMSPSDRNSRWANSGIVVEIRLDDLAGYAQHGIFAGLEFQAELERLAKQHGGYGV
                     QAPAQRLVDFVEGRFSSNLPACSYHPGLNSSEMHLWLPEHIGFRLREGFKAFGKKMKG
                     FLTNDAVILGVESRTSSPIRIPRDPETLQHIQVKGLFPSGEGAGYAGGIVSSALDGNI
                     VADAVSRYVS"
     gene            8097..9080
                     /locus_tag="PK049_09795"
     CDS             8097..9080
                     /locus_tag="PK049_09795"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOK61890.1"
                     /translation="METDALIVLLTILLPLSFIAERITNLIKLFIPDGFLGLGNVRYK
                     EDDPIMEKKRERRIFAISLISGELVSFSLKADLFAIINQGTFGWNTPTTGYSWIIGCL
                     FTGFFLSWGSKFWHDLLEILLEMKNIRRVNVKAKTLEVKQKEVEVAEKASEQPVCLPV
                     EAKPEVKEDKLSLNRIKMLHPKLRQEVEQIYRELVKQRIPVRITDTLRTFKEQEALYA
                     IGRTKPGKIVTNAKPGQSYHNYGLALDFCLLTNDGREATWNRAADLNLNQKADWDEVV
                     ALFKHYGWEWGGDWVSFKDYPHLQKTFGFSTAKLLDMHSNANVDSEGYVKI"
     gene            9085..10422
                     /locus_tag="PK049_09800"
     CDS             9085..10422
                     /locus_tag="PK049_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61891.1"
                     /translation="MQKSSKWLTVLAAIVLLLFAYRVVIEFDKHCSKKPNPEFKRYIE
                     LQQKQLDSVLTRLGEEGIISTSKIELQSPSRCFTSNFEGAEVLVVGLQHHDSIYGTES
                     RKPYYTYGCNYVLFDPDHPIYGLPDIEMKVGPVGFYQPELNGLEKLAGLGINSKPRPY
                     RVFRKPVYDSTGKKLNVFEMQLWLTQFKVALTVVSDRDKPQVSPDNESMKVKKYPGKW
                     YSDTRPMICINDLKDKEEYMNHRYGDITFILEVNPNASPWYIKGNNQQTLRPDIAVGA
                     IVCNEMVKRPEKENQIHINIQKGMLAPLYLDPFETNANLNDLNSYELQLAEKLDEYRR
                     FNIWNRKYYFKIHSKNIGARKTGILWNAKTFDEQIEFTFLLPLLVVGSWDVQLPWEVI
                     PAMEAPQPYYKSFSIKNLLPDWGLGGIGKGLSSLILVGLAALCIFWLLPFFRR"
BASE COUNT         2846 a         2143 c         2451 g         3022 t
ORIGIN      
        1 gccctttcag ggcgatttat gtgtgcgtct aaagacgtgt gctaactagt gagttcttaa
       61 atcttaaatc gagatccctc gactacgctc gggatgacaa tggagttgta aatgttacgc
      121 cccttcgggg ctgaagacgt tgttcgctat tcgtgaatcg ttgatcgagt atatcacgtc
      181 acacgctctg tgtaacaacg ttttacattg tgtaactctg tgatacttgc tgttgctagt
      241 ttcatgagaa ggttgtcatg ttgagctaag cgaaacgact catggggtcg attagagatc
      301 cttcgctccc cgctagggcg ggacaggccg ctcaggatga caggcatatt tttgttaact
      361 aaacgacatt tattgatatt tagtttttac tcaagggcgg atagggtgga gtggaagtta
      421 atttttcatc cacaatatct gatagtagaa tacttagctc attaatagct tttttgtcat
      481 caattatctc tatcacttct tccttattaa gaaaattaag aataaaaaag gaatcaaagt
      541 cgatgtttaa tctcaaatac tgatagaatc tttggttttc aagttcaaag cccattgatt
      601 ttgcatactt cttcaatctt ttttccagtt cttcttttgt tagtttagcc atgccttttt
      661 gtatttcaaa acagaatcta atttaattga taattctgat acttgcaagt gtgattatca
      721 tatactccct gattttaatt gaaaatagag aggataacag tacataatcc ctaagcactg
      781 aatcaaaagc atgttggtta tatatataaa aaaagcgcct aaaggcgctg ttaaaataaa
      841 aaaccaaata aaaaagatta tggtttagta tcatttaact gctactcatc gttgctattc
      901 agcaagccga tgtagtatag cagggtggca agtgagccaa gtgctgctac aacgtaagta
      961 agcgcagccc atttaagggc atcgcgggcg tacagagcgc cctcgccata ggttaagcca
     1021 gatgagttga gccacgcaag cgccctgcgt gaggcatcca tttcaactgg gagggtaata
     1081 atgctgaaaa gggttgttaa cccgagcatg attactccta taagtaagat actaggcatg
     1141 gtttggagca gaataacacc tgccagtaaa acccattgaa cccaacgcga ggctacgctc
     1201 acgacaggaa ccaaagccga acgcatctgc aggaaagcat agctctgggc atgctgaatg
     1261 gcgtgcccgc attcgtgagc tgccacagct gcagcagcaa tgctgcgtcc gtggtataca
     1321 tcggggctaa ggtttacggt tttatttgcc ggattgtagt ggtcggttag ctcaccatct
     1381 accgatatta cttttacatc gtaaatacca tggttgtgga gcataagctc ggccacctcc
     1441 tttccgctaa gtccccgagg taaggccatg cgcgagtaac gcgcaaactt cgacctaagc
     1501 tgcatggaaa ccatgaagct tagcacgaaa aagatcatca ttatcaggta aattcccata
     1561 acgagtttat ttattgtttc ttttcacgta tcaactagtt aagacaaatt gcttacacgt
     1621 ttcgattcac gtagtaggcg ttagcctgag ccactggtgt tacctctaaa tcgttaatgc
     1681 aaacgtgggg tgggcgagtt agaacaaagt gaatggcatc ggcaatatcc tcggcaaata
     1741 gtggggttag tccaacatag gtgtttttgg ctttctcgct atcgccctta aagcgaacaa
     1801 tggagaactc ggtttccagc aatccggggc gaatttcggt aaccttgata ttgtgcttta
     1861 gcatatcctg ccgcataccc tgcgagagtg catgcatggc atgcttggtg gcacagtaaa
     1921 cgtttccgtt ttcgtaaacc tgtgttccag caatcgaacc aatatttaca atgtgacctt
     1981 tcccccgttc aatcatcatg ggggcaacct tacgggtgat gtaaagcact ccttttatat
     2041 tagtgtcaat cattcgttcc cagtcatcaa ttactccgtc ctgtatgtgg ttaagtccaa
     2101 ctgccaagcc ggcattgttt accagaacat caatttttct ccagcgctca gggagcgaat
     2161 caatggcact gttaacctca tccaggttac ggatatcaaa gttcagaata tgaacatctt
     2221 tcttatactt tacttcaatc tcttttttta gctcttcaag cctttcgagt cttctgcctg
     2281 taatgataag gttaaactca tccatggcca acctgagggc agtagctcgt ccaatgccag
     2341 aggtagcacc agttatcaat gcaattctca taatatcaat agtataggtg tgttagtaag
     2401 cgtttcatgt taactcaaaa ttagaatata agtcagtaac tacacaatgg cttaaggttt
     2461 tttaaaactt ttttggtaaa acatcgataa tgaatgttta actgctattt tttcgatttc
     2521 agcttatgtt acttgagtta atgttcgtat ttttggcagg aaatttataa agtagaaaaa
     2581 ttagaaagtt atgccaaacc gaacagttac cgttgaaacc gcccgcgagc tagggcttct
     2641 tcctgaagag tttgagatga ttaagaacta cttgggacgc accccaaact acactgagtt
     2701 aagtatatac tcggtaatgt ggtcggaaca cgcatcgtat aagaattcaa tcaagtggct
     2761 aaaaacatta ccccgtaaag gttccgctat actggcagaa gctggaacgg agaatgcggg
     2821 tttggttgat attggcaatg gtttggcatg tgcatttaag attgaatcgc acaaccaccc
     2881 ttgtgctgtt gaaccctacc agggtgctgc tacaggagtt ggaggtatta atcgcgatat
     2941 tttcaccatg ggcgcacgcc cggttgccca gctaaactca ttacgatttg gcaaccttaa
     3001 gctcgataga actcgctggt taattaaggg agtggttaag ggtattggtg attatggcaa
     3061 tgcctttgga attcctgtgt tggcaggcga agttttcttc gacgattcat tcaacatgaa
     3121 tcctctggta aatgccatgt cggttgggtt ggttgagaag gacaaggtgg tttcagctgt
     3181 atcaaagggt aagggtaatc cggtttacat tgttggttca gccaccggaa aggatggtat
     3241 tcatggtgcg acattcgcat cggccgatat aaccgaggac tcagccgagg atattccttc
     3301 aattcaggtg ggtgacccct ttcaggaaaa gatcttgctg gaagcaaccc aggaggtgat
     3361 caggactggt gcactggttg gaatgcagga tatgggtgca gccggtatca tatgttccac
     3421 ttcggaaatg tccgaaaaag gtggacatgg catgcgaatc gatttggata aggttccttt
     3481 aaggcagaag aacatggagg cctgggaaat cttgctgtcc gaatcgcagg agcgaatgct
     3541 catggtggtt cagaagggtc gtgaggccga tgttgaggct gtacttaata agtgggatat
     3601 tagctacgcc attattggtg aggttaccga gggcgatacc ctagagtttt actaccatgg
     3661 agagctggtt gccaaggttc ccgcttcatc gttggttctt gggggtggcg ctcccgttta
     3721 cgatagggag taccgtgaac cagtttacta caaggagtat aaaaagttcg atattaataa
     3781 agttcctgaa cctgccgatt tgattgatgt agcccgtttt ctggttactc accccaatat
     3841 tgcatcgaaa cgatgggttt atgagcagta cgacaccatg attggcactg ggaatatgac
     3901 taccaacttc ccctccgatg cgggtgtata caacctgaag ggcaccaata aggcgcttgt
     3961 gatgactgtt gactgcaatt cgcgttacgt taaggccgac cccatggttg gaactatgat
     4021 tgcagtatcc gaggcagccc gaaacatctc ctgcactggt gctaaaccct tggctgttac
     4081 taactgcctc aactttggta acccatataa ccctgaagtt tactggcagt ttgtacaggc
     4141 agttaagggg atgggtactg cttgtgaaaa gttcgacacc cctgttaccg gcggtaatgt
     4201 tagtttttac aaccaagcat caattggagg caaggaggag gccgttttcc ctacaccaac
     4261 cattggtatg attggtttac ttgaaaataa gaatcaccat acaacgctgg ctttcaagga
     4321 gaagggtcat atgatttatt tgattggtaa gtcagtaaac gatattgcat cgtccgaata
     4381 cctgtacagc taccacggtg tcaagcagtc gcctgcgcct tactttaacc ttgaggagga
     4441 gtatgaggtt caacgtgcgg ttcgcgaact tattgcacgt aacctgattc aatcggcaca
     4501 cgatatttcc gatggaggac tattcattgc gcttcttgaa agcgccatgc ctcgcaactt
     4561 gggtttcgat atcactaccg atgccgaaat tcgtaccgat gccttcctgt ttggtgaggc
     4621 ccaagggcgt attgtggttt cggtatcgcc atcgcgcgaa acccagttta ttgactttat
     4681 ggtagcaagt aaggtgcctt tctcaaccct tggtcatgta accaagtcgg aaattagagt
     4741 cgatgatgtt tcgtttggct ttgtagccga tttaaagaaa atttacgact cctcgcttga
     4801 gcagctgata aatgggtaaa agtccaaata ctgttgttga taaacgaacc agtagcgttg
     4861 cggcaatgtt taatagcatt gcgccaagct acgattttct taaccatctg cttagccttg
     4921 gcattgataa gctttggagg cgacggcttg taaaaaggct gctacgtaac aatcctgggg
     4981 aggttttgga tgtggcaacc ggaactgctg accttgcact ggtcatggct aaaaaatccc
     5041 ctactgttaa gatagttggt atcgatatct ctgaatcgat gcttgccatt ggaatggtta
     5101 aggtaaaaaa atggaagttt ctggagcgca ttacccttaa aaaggcctcg gcgctctgta
     5161 ttccctcacc cgataaccat ttcgatgctg ccatggttgc atttggtgtg cgaaactttg
     5221 aggacttagc ccaaggacta atggagattt gtcgtgtgct taaacctggc ggagaactgg
     5281 gtgttttgga gttctccatg ccccgccact ggccgtttaa tgctttatat cgattttact
     5341 ttctaaaatt cttaccgtgg gttggtggaa aggtgtcggg caaccgacag gcatatacct
     5401 atctccctga atcggtgctt tcattccctc agggtgaagc ttttgtccag atacttaagt
     5461 tagcgggttt taacgatgtg gaaatcaggg ttcaaacctt tggtattgcc accctttata
     5521 ttgcgaaaaa gaaaaatcaa taatattttt gactggttac cgtttaatca gagtcgttac
     5581 taaaactgtg aatcgcactt gaaacgtttt tttgcattac tattactttg ttttttggta
     5641 gttggcaact catttggtca attcaaacgg cccacattgc taaacgaccc cgactacgac
     5701 gtagggaaac cgctcaggtt tggtttctct attggcgtta atgtaatgga ttttgatgca
     5761 gttaaccgaa ccgctcagtt caatgccgat ggctcaaagt actttgcaga ggtaacccat
     5821 atttcggcag gtcttaacgt aaatgccatt ggcgacctcc gtattgcccg cgatattcat
     5881 ctacgttttc tgcctggata ctcttttggc cagcgcgatg taaatttttt taaggttgat
     5941 gccgacagta ccgtttcgct ggctacaacc atgaagatgg agtcgaactt catcgatttg
     6001 cctgttggaa ttaagttctt atcggagcgc aacagtaatg tccgccccta cttgtacctg
     6061 ggaactgacg ttagaattga tttagccgca tacaagcgcc tcaaggttga aaagggcgtt
     6121 ttgttaaggc tacaaaagtt cgactactac tatgaggttg gctttggtgt cgatttcttc
     6181 ctccaatact ttaagttttc ggctgagatt aagtggtcgg ccggtttgct taatatgtta
     6241 tcgagcgatt atgttgatga gggaaagatt taccacgatg ccctgggaag tttaagatcg
     6301 aagttcctga ttgtttcgtt ccactttgag taggtttaaa cctttttgtc cttatctgtg
     6361 ttaatgcttt cttattagag tttttcctta tgccacatga tatctctatt gtgttaaggc
     6421 ccgatgaggc ggcaaatgaa acagcagttg ttagagcaat tgcacggaaa attgatatac
     6481 caaccgattc aatccggcat tactatataa cccggaaatc aattgatgcc cgtaagggta
     6541 aggttctggt taacctttgc gttagggttt ttattggcga ggaaaagatt attgatgaac
     6601 gaccgatttt caattacaat gatgttcgta atgcaatgcc ggttattatt gttggggcgg
     6661 ggccagcagg attatttgct gcattaaggt taattgaact tggttttaaa ccaattgtaa
     6721 ttgagcgtgg caaatccgta agccatcgta agcgcgatat agctatgcta aatcgggaac
     6781 atcagcttaa ccccgattca aactacgcct ttggcgaggg tggtgccgga accttttccg
     6841 atggcaagtt atacacccgt tccaaaaagc gaggcgatta caggcgcatt cttcaggctc
     6901 tggttgatca tggagccgat ccggcaatac tatacgaggc tcacccgcat attggaacca
     6961 acaggctacc cggaattata actcgtatta gggaaactat tcttcagcat gggggtgaag
     7021 tgctttttag tagcagggtt acagagataa ttatagattc cggtcaagcg gttggtgtta
     7081 aacttgccga tgggcaaagg ataaatggca aagccgtaat acttgccact gggcattcgg
     7141 ctcgcgatat ttatcacatg cttcacaatc aaagtattgc cttggaggct aaaccgtttg
     7201 ccatgggggt gagggttgaa catccacaag cacttatcga tgccattcaa taccatggca
     7261 taccccgaag cgagtattta cctgctgctg cgtattcact ggtacatcag gttgagggac
     7321 ggggggttta ctcattctgc atgtgtcccg gtggttttat tgtgcctgcc gctacaggaa
     7381 ccaatgaggt tgtagtcaat ggaatgtcgc cctcggatag gaattcacgc tgggcaaact
     7441 caggaatagt tgttgaaatc cgtttagatg acttggctgg ctatgctcag catggcattt
     7501 ttgccggact tgagtttcag gctgaattgg agcgtcttgc taagcagcat gggggatatg
     7561 gagttcaagc tcccgctcag cgtttggttg acttcgtaga agggcgtttc tcatcaaacc
     7621 ttcctgcctg ctcataccat cctgggctaa actcgtcgga gatgcacctt tggctacccg
     7681 agcatattgg ttttaggctc agggagggtt tcaaggcttt tggtaaaaag atgaagggtt
     7741 tcctaaccaa cgatgctgta atactgggag tagaatcgcg gacatcgtcg cctatccgta
     7801 ttcctaggga tcctgaaacc cttcagcaca ttcaggttaa agggcttttc ccttcgggtg
     7861 agggtgcagg ctatgccggc ggtatagtgt catcggcctt ggacggtaat attgtagctg
     7921 atgcagtttc ccggtatgtt agctagagga actgattttt gcgatttttt accctcaaca
     7981 cctatgcgaa ttctgaaaat cttaatagtt tgtcaattta tcatccaaaa aaaatcaatc
     8041 aaacgtttta aatcatttta atttagcgta acttgtatcg gtttaaaaac aatcctatgg
     8101 aaaccgatgc actaattgtt ctcctaacaa tcctacttcc gttaagtttt attgccgaac
     8161 gtatcacaaa cctgataaag ctgtttatac ccgatggctt tctgggacta ggtaatgttc
     8221 ggtataagga ggatgatcca attatggaga aaaagcgtga acgccgaatt ttcgctatta
     8281 gtcttatctc tggcgagtta gtatcatttt cgcttaaagc tgatcttttt gcaattatca
     8341 accagggaac gtttggatgg aatactccaa caacaggcta ttcatggatt attggatgtt
     8401 tgtttactgg ctttttcctg agttggggaa gcaaattctg gcacgatttg cttgagatac
     8461 tcctcgaaat gaaaaacatc cgcagggtaa atgtaaaagc caaaacgctt gaggtaaaac
     8521 agaaggaggt tgaggtggct gaaaaagcct cggaacagcc ggtatgcctg ccagttgagg
     8581 ctaagcccga agttaaggag gataaactgt cgctcaaccg tatcaaaatg ctccatccaa
     8641 agctcaggca ggaagtggag cagatttacc gtgaactggt taaacaacga ataccagtaa
     8701 gaataaccga taccctacgc actttcaagg agcaggaagc actttacgcc attggacgga
     8761 ccaagccggg taaaatagta actaatgcta aacctgggca atcctatcac aactacgggt
     8821 tggctctgga tttttgcttg ttaaccaacg atggacgtga agccacctgg aaccgggcag
     8881 ccgatttgaa ccttaaccag aaagccgatt gggacgaggt tgttgcacta tttaaacact
     8941 atggctggga gtggggtggc gattgggtat cgtttaagga ttatccgcac ctgcaaaaaa
     9001 ctttcggctt ttctactgct aagctcctcg acatgcatag taacgcaaat gtcgattcag
     9061 aaggatatgt aaaaatctaa cctcatgcaa aaatcatcta agtggcttac tgttctagca
     9121 gcaatagttt tgctgctgtt tgcctaccga gtagttattg agttcgataa gcattgtagc
     9181 aaaaaaccga acccagagtt taaaagatac attgaactgc agcaaaagca gctggatagt
     9241 gtgctaaccc gactgggcga agagggaatt atttcaacat ccaaaattga attgcagtcg
     9301 cccagcaggt gttttacatc aaactttgag ggggctgagg tgcttgtggt tggacttcag
     9361 catcacgata gtatttacgg taccgaatcc cgaaaacctt actacactta cggctgcaac
     9421 tacgttttgt ttgatcccga tcaccctatt tacggattac ctgatattga aatgaaggtt
     9481 ggtcccgtag gtttttacca acccgaactc aatggtttgg aaaagttggc aggtttgggg
     9541 attaactcaa aaccacgccc ttatagggta ttccgaaagc cggtttacga tagcaccgga
     9601 aaaaaattaa atgtttttga aatgcagctc tggctaaccc agttcaaggt tgcattaacg
     9661 gtagtaagcg atagggacaa accccaggtt agtcccgaca atgagtcgat gaaagtaaaa
     9721 aaatatccgg gtaagtggta ttccgatacc agaccaatga tttgtataaa cgaccttaag
     9781 gataaagagg agtacatgaa tcaccgttac ggtgatatca cctttattct tgaggtaaat
     9841 cccaatgcat cgccttggta cataaaaggg aataatcaac aaacattaag gcccgatatt
     9901 gctgttgggg ctattgtttg taacgagatg gttaaacggc ccgaaaagga aaatcaaatc
     9961 catatcaaca tccaaaaggg aatgcttgcg cccctttacc ttgacccatt tgaaaccaat
    10021 gcaaacctaa acgatttgaa tagctatgaa ctgcaacttg ccgagaaact ggatgaatac
    10081 aggaggttta acatttggaa cagaaagtac tactttaaga tacattcaaa gaatattggt
    10141 gcaaggaaaa caggaatact atggaatgca aagacctttg acgagcaaat tgaatttaca
    10201 tttctgttac ccttacttgt tgtgggcagt tgggatgttc agctaccctg ggaggttatc
    10261 cccgccatgg aagcacccca gccttattac aaaagtttta gcattaaaaa tcttttaccc
    10321 gactggggtt taggaggaat tggtaaaggt ttaagtagct tgatattagt aggtttagct
    10381 gcattatgta ttttctggct tttaccattc ttccgcaggt agatttattg atgtaatgtc
    10441 cctttaaatt ctgagagtat ta
//
LOCUS       DAOSAL010000080        10104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_4370_length_10104_cov_50.802368, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000080 DAOSAL010000000
VERSION     DAOSAL010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 10104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10104
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4370_length_10104_cov_50.802368"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="PK049_09805"
     CDS             <1..382
                     /locus_tag="PK049_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61892.1"
                     /translation="KVADLFYNGDYNKAAREIGRFSADIALKGIYKIFVRISSPQFVL
                     SRASSIFSTYYQPADIKVVESSDKKAVLQLEKFHVDEKLIMERIAGWIEHTLEITLKS
                     PLKVDVEQIVNGKNLTARITAMWQ"
     gene            442..1209
                     /locus_tag="PK049_09810"
     CDS             442..1209
                     /locus_tag="PK049_09810"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HOK61893.1"
                     /translation="MLGLAAKSLVPVRKEPNETTEMVNQLLFGEKVTILENYKQWYRI
                     ESTLDSYPGWVDSRLIDIVADTSSEKELTNFSVLSSLAEAIKVDDNSRYLLSPGSILY
                     GYSGKKFHLNGSNFELTANIPSLPTNPLDHIIKTAQAFINTPYLWGGKSIFGIDCSGL
                     VQVCFRTAGVFLPRDAWQQVQNGQTVEFVDHAQPCDIAFFDNEEGRIIHVGILINPNE
                     IVHSSGYVRIDKFDHQGIYNPQLGIYTHKLRIIKRVL"
     gene            1504..1956
                     /locus_tag="PK049_09815"
     CDS             1504..1956
                     /locus_tag="PK049_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008822135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOK61894.1"
                     /translation="MYTYEYPRMLVTVDAVIFKVDINYKPTHLLLIERGNEPYKGHFA
                     LPGGFPDIKELLADAAARELFEETGIAGVELKQIGAFDDIDRDPRDRNIAIAFWGVVS
                     DDKISPTAGDDAAKAQWFPLDNLPPLAFDHSKIVKAALGCLKRGFPEQ"
     gene            complement(1962..3239)
                     /locus_tag="PK049_09820"
     CDS             complement(1962..3239)
                     /locus_tag="PK049_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOK61895.1"
                     /translation="MEDKVIERSYLDMELLRFTTAGSVDDGKSTLIGRLLYDSKAIFQ
                     DQLEAIERASIKKGEEYINLALLTDGLRAEREQGITIDVAYRYFHTPKRKFIIADTPG
                     HVQYTRNMVTGASTANAAVILVDARHGVIEQTIRHAYIASLLRIPHVIVCVNKMDLVD
                     FKQDVFDSIQQKFHEFATHLDLHDVRFIPISALLGDNVVDRSKNMDWYQGPTLMYLLE
                     NLYISNDYNQLDARFPVQYVIRPQSIEFHDYRGYAGRMASGTFRVGDRVKVLPSGFTT
                     TIKGIDFWTDHLDSITAPQSGTIILEDDLDVSRGCMIIPEENGIKQGQDIELMICWLG
                     DKPMVPGGKYALKHTTNDLRCMVKEVKYKININTLEKDYNDKSVGMNDIACITIRTTK
                     PLFYDSYRQNRVTGSLILIDEATNVTVGAGMIV"
     gene            complement(3257..4165)
                     /gene="cysD"
                     /locus_tag="PK049_09825"
     CDS             complement(3257..4165)
                     /gene="cysD"
                     /locus_tag="PK049_09825"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HOK61896.1"
                     /translation="MSNKVPINHLRELESEGVFVIREVVSQFENPVMLFSGGKDSIVM
                     FHLARKAFYPAKVPFPLMHIDTGHNFQETLDFRDWLVQETGVQLIVRYVQDSIDKGRV
                     VEETGYNASRNKLQTVTLLDAIEELKIDCAMGGGRRDEEKARAKERFFSHRDEFGQWD
                     PKNQRPELWNLFNGRKNMGEHFRVFPISNWTEMDVWQYILMENIAIPSLYFSHEREVF
                     DRDGVLYAKTPFLKMKDTEKAEKRIVRFRTIGDATCTGAIESVASSVEDIIREVAATR
                     VTERGGRADDKRSEAAMEDRKIEGYF"
     gene            complement(4351..4887)
                     /gene="cysC"
                     /locus_tag="PK049_09830"
     CDS             complement(4351..4887)
                     /gene="cysC"
                     /locus_tag="PK049_09830"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HOK61897.1"
                     /translation="MSYKHPITVWITGLPASGKTTLGKALQGRINQLKIPVVLLDGDE
                     VRKGLCSDLGFSPADRTENIRRVANMAALLNSQGVVTICCFVSPLISMREMARSIVGS
                     KNFFEVFTDAPVEVCQSRDIKGNYSKAINGSLPNFTGISDSYEAPMNPSLRLNTAELS
                     EGEAADILYDHFLRWVDK"
     gene            complement(4893..5534)
                     /locus_tag="PK049_09835"
     CDS             complement(4893..5534)
                     /locus_tag="PK049_09835"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HOK61898.1"
                     /translation="MKQILSLLFISSSLTLFSQEVNPDGIVRSFSNKMQEIKSLSATF
                     SFTLENLQERITDTHQGKILAKGKMFYLDLMGMEVFSDGQTKWQYIKEAKEVTISKMN
                     IKENSFLDDPMKLFKDYDKNFKYKYIGQQKSKNRVLHEVDFFPRDLNLPYSSVKLQFD
                     ANTLEPYLIRYQGKDGNNYIIQIKNFKSNVPLRDDRFKFDVEKHKGVEVIDMR"
     gene            complement(5576..8017)
                     /locus_tag="PK049_09840"
     CDS             complement(5576..8017)
                     /locus_tag="PK049_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HOK61899.1"
                     /translation="MARKDKGTDVEDSDSKDVSNDSLFKDERIRFTLGLFLILLGVYI
                     FLSFLSFLFTWKVDQSIEWVSIFSSTEQKIGNWGGKLGASLSNQFINHWFGITALAFP
                     LIFMVLGLKLLKFIRGRVTKFIFKVLLGILVGSLTLGFLFKELGGYLGSGLGGAHGLF
                     VSEWLSTVIGKVGAAFLVLIALIAYIIYVMPKSLYHFQSGTFFLFGSLRNFVVKGKQK
                     ESQSEKHGEEDFKQAIVEVAPVDISNERIKEKVDEFDDTDEIDDLDDELDLDVQDEYD
                     GRETKTVTESTIEDNFTIERNEEKELAISDINEQELYDPTLELSSYQKPLIELLEDYK
                     NTESPVTNEMLVENKNKIVETLSHYKIQIDKIKATIGPTVTLYEIVPAPGVRISKIKS
                     LEDDIALSLSALGIRIIAPIPGKGTIGIEVPNQNPEVVSMHSIIKSQKFQECKYDLAI
                     ALGKTISNEIFMLDLTKLPHLLVAGATGQGKSVGLNAIITSLLYKKHPAEIKFVLVDP
                     KMVELTLYNKLERHFLAKLPDSPEAIITDTQKVIYTLNSLSVEMEDRYKLLNLAKVRT
                     IKEYNQKFVARRLNPNNGHRYLPYIVVIIDEFADLIMTAGREIEMPLGRLAQKARAIG
                     IHLIIATQRPSTNIITGVIKANFPARIAFRVSSMVDSRTILDTPGANNLIGRGDMLVS
                     AGSDLVRVQCAFVDIPEIERITDFIGNQPGYPHAYELPEYVPEGQDGPVDVDLRKRDE
                     LFEDAARLVVQTQSGSTSLIQRKFSIGYNRAGRIMDQLEAAGIVGPADGGKPRQVLIV
                     DEVSLERILNTLSVD"
     gene            complement(8077..8772)
                     /locus_tag="PK049_09845"
     CDS             complement(8077..8772)
                     /locus_tag="PK049_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61900.1"
                     /translation="MNRSVYQLVGVAVILFSLSSCISKSPKEISEGRIEYDIEYDTLT
                     LSRIDKKILPSSLVVRFCNNNTINTIDGLSGAITISIISQPSKKEFTTLLKVFNKKLY
                     HSEPYTNGHYPALYARIPKVNIDTLVRDCDYIGYKCKSVMGYFADNPDNRFEIIYTKQ
                     IAIDNPNLNTPFENIDGVMLGFNLRFNNLEMKLRARNVAKENINNDAFKIPKDYKKVD
                     FQTMTDLIYLLQQ"
     gene            9002..9961
                     /gene="arcC"
                     /locus_tag="PK049_09850"
     CDS             9002..9961
                     /gene="arcC"
                     /locus_tag="PK049_09850"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HOK61901.1"
                     /translation="MSKLAVVALGGNALLRGNQVGTIDEQEQNTTDTLENLVYLIKEG
                     YNLVISHGNGPQVGNILMRNDAGEQLYGIPQMPLDICVADSQGGIGYMIERMLRNVLR
                     KHGIDREVCCLVTPVIVDHDDPAFTNPTKRVGRIYDKAQADALAAEKGWIFKEEVKDT
                     GSGWRRVVPSPKPKGILNIKVIDQLVRQGIIVIASGGGGVPVYIDEEKNVRPAEAVID
                     KDLASALMAAEIKADEFYILTDVPFVYLNYKKPNEQKLEFLNYADTMKYLEQGMFGEG
                     NMAPKIKACLDFIKRGGKMSVITEATKLEDMSYGTKITMEYED"
     gene            9989..>10104
                     /locus_tag="PK049_09855"
     CDS             9989..>10104
                     /locus_tag="PK049_09855"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005318430.1"
                     /note="catalyzes the formation of ornithine and
                     carbamylphosphate from citrulline in the arginine
                     catabolic pathway; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase subunit F"
                     /protein_id="HOK61902.1"
                     /translation="MSFNLRNRSFLKLLDFTPKEIQFLLDLSADLKKAKYAGT"
BASE COUNT         2968 a         2218 c         2044 g         2874 t
ORIGIN      
        1 aaaggtagcc gatttgtttt acaatggcga ttacaataag gcagcacgtg aaattggaag
       61 gtttagcgcc gatatagccc tcaaaggcat atataaaata tttgttagga taagttcacc
      121 acagtttgta ttaagtagag catcaagtat attttccact tactaccaac ctgccgacat
      181 taaagtggtg gaaagttccg ataaaaaagc agtacttcag cttgaaaaat tccatgtcga
      241 cgaaaaactc ataatggagc gaattgcagg ctggattgag catacccttg aaatcacact
      301 taaatcaccc ttgaaggtcg atgtggaaca aattgtaaac gggaagaacc ttaccgctcg
      361 gataactgcc atgtggcaat agctacaata gttattgaat gaaacctgat aattgctata
      421 tttacaaaaa aaatagcaat tatgctggga ttggcagcta aaagtttggt tccggttcgc
      481 aaggaaccca atgaaaccac tgagatggtt aaccaactac tttttggcga aaaggttaca
      541 attcttgaga attataagca atggtataga attgaaagca cccttgacag ctacccggga
      601 tgggtggact ccaggctaat tgatattgta gcagacacta gcagcgaaaa ggaacttacc
      661 aacttttcgg ttctctcatc actggccgag gccataaagg tagatgataa ctcaaggtat
      721 ttacttagtc caggatcgat tctttacgga tattctggaa aaaaattcca cttaaacggc
      781 tcaaattttg aacttactgc caatattccc tctctgccca ctaatccttt ggatcatata
      841 atcaaaaccg cccaagcatt tataaacacc ccgtacctat ggggcggcaa atcgattttc
      901 ggcatcgatt gttctggcct ggttcaggtt tgcttcagaa ctgccggggt attcctccca
      961 cgcgatgcgt ggcaacaggt tcaaaacggc caaactgttg agtttgttga ccatgcccaa
     1021 ccctgcgata ttgctttctt cgataacgaa gagggacgta taatccatgt tggtatacta
     1081 atcaatccca atgaaatagt acatagctcc ggttacgttc gaattgataa atttgaccat
     1141 caaggaattt acaacccgca actgggcata tacacccaca agcttaggat tattaagcgg
     1201 gtactataag ccataatgga acggaagaca atggcttggt gggccaaacg aaagaaatct
     1261 aaaagaatgt aatccaattg attccaatgc attctctatg caagtctgca taattatagt
     1321 tttgcttgac attaaaacat atttttggta tctttcaaaa tccgttctaa actcaaccgt
     1381 atgctaaaac accaactttt gatagccatt gccattattg catttgcaat ttgggttgct
     1441 cttagaaaac gcgatcaaaa aaaatcggac aacgaagagg accctgataa tgaatagttt
     1501 agcatgtata cctacgagta cccccgaatg cttgtcacgg tcgatgccgt aattttcaag
     1561 gtcgatataa actataagcc aacccacctt ctactcattg aacgtggcaa cgaaccctac
     1621 aagggacatt tcgctttacc tggaggcttc ccagatatta aggaactact agctgatgcc
     1681 gcagctcgtg agctatttga ggaaaccggg attgctgggg ttgagctaaa acagattggt
     1741 gcattcgatg atattgatag agacccacgc gatagaaaca ttgccattgc cttttgggga
     1801 gtggtaagcg atgataaaat tagtccgact gctggcgatg acgctgcaaa agcccaatgg
     1861 tttccgcttg ataatcttcc cccgctggca ttcgaccatt caaaaatagt taaggcagct
     1921 cttggctgcc ttaagagagg ttttccagaa cagtaaactg cctaaactat cattccggca
     1981 cctactgtta cattagtggc ttcatcaata agtatcaaac ttcctgtaac acggttctgg
     2041 cggtacgagt cgtaaaacaa tggtttggtt gtgcgaatgg taatgcaagc aatatcattc
     2101 atccctaccg atttgtcgtt gtaatccttt tccagggtgt tgatgtttat cttgtacttc
     2161 acctccttga ccatgcagcg caaatcgtta gtggtgtgct tcagggcata cttaccaccg
     2221 ggaaccatgg gtttatcgcc taaccaacat atcattagct caatgtcctg cccctgctta
     2281 atgccattct cctcaggaat aatcatacac cctctggata cgtccaaatc gtcctccaaa
     2341 ataattgttc cggattgcgg agcggtaatt gaatcaaggt gatcagtcca aaaatcgata
     2401 cccttgatgg tagtggtaaa gcccgagggt aaaactttaa ccctatcacc aaccctaaat
     2461 gtaccacttg ccatccgtcc ggcataaccc ctgtaatcgt ggaactcaat ggactgtgga
     2521 cgaataacat actgaacagg gaatcgagca tcgagctgat tataatcatt tgaaatatac
     2581 aggttctcaa gcaggtacat cagtgtaggg ccttgatacc aatccatatt cttggaacgg
     2641 tcaaccacat tatcgcctag aagagcgcta atgggaatga atcggacatc gtgcaaatcg
     2701 agatgcgttg caaattcatg gaacttctgc tgaatactat caaaaacatc ctgcttaaaa
     2761 tcaactaaat ccattttatt cacgcatact atcacgtgag gaatacggag aagcgaggca
     2821 atgtaagcat gacggatggt ttgttcaatc acgccatggc gggcatcaac caggataacg
     2881 gctgcatttg cagttgaggc accggttacc atgttgcggg tatactgtac gtgacctgga
     2941 gtatcggcaa taatgaactt gcgcttgggg gtatggaaat aacggtatgc cacatcaatg
     3001 gttataccct gctcgcgctc ggcgcgcaga ccatcggtta gtaaggcaag attgatatac
     3061 tcttcgcctt tctttatact tgcccgttca attgcttcca gctgatcctg aaagattgcc
     3121 tttgaatcgt atagtagtcg tccaatcaga gtgcttttgc catcgtcaac actgccagca
     3181 gtagtaaacc gcaaaagctc catatctagg tatgaacgtt caattacttt atcttccatt
     3241 ggaaaatgat ttaaaactaa aaataacctt caattttacg atcctccatg gctgcctcgg
     3301 agcgtttatc gtcagcacga ccgccacgtt cagttactcg cgttgcagcc acctcacgaa
     3361 taatatcctc aacgctactt gcaaccgact caatagcacc tgtgcaagtg gcatcgccaa
     3421 tggtacggaa acgaacaatc cgtttttcgg ctttttcggt atccttcatc tttaagaagg
     3481 gagttttggc ataaagcaca ccatcgcgat caaaaacctc acgttcgtgc gagaagtata
     3541 gcgagggtat agcaatattt tccataagta tatattgcca tacatccatc tcggtccagt
     3601 tgctaattgg gaatacccta aaatgctcac ccatattctt gcgaccatta aaaaggttcc
     3661 agagttcagg gcgttggttt ttgggatccc actggccaaa ctcatcgcga tgcgagaaga
     3721 aacgctcctt tgcccttgcc ttttcctcat cgcgacgccc acctcccatg gcacaatcta
     3781 tttttaattc ctcaatggca tcgagcaggg taacggtttg caacttgttt cggctggcat
     3841 tgtaaccggt ttcctccact accctaccct tatcaattga gtcctgcacg tatcttacaa
     3901 ttagctgcac tccggtttcc tgcaccaacc aatcccgaaa atctagtgtt tcctgaaagt
     3961 tatggccggt atcgatgtgc attaggggga agggaacctt tgcgggataa aaagccttgc
     4021 gggctagatg aaacatcact atggaatcct ttccaccgct gaaaagcatc acagggtttt
     4081 caaactgtga aaccacctcg cgaataacaa acactccttc cgattcaagt tcgcgtaagt
     4141 ggttaattgg cactttatta ctcatatcaa aatcctttta attacttcaa ttttagcaac
     4201 ttgagagatt acatcaggta acccttaaca tgcttgccta cttggtgttt ttccctaaac
     4261 aatattccta tagcttttca tttgcctcta tcgctttcaa caccctataa gcatggcaaa
     4321 gatacaccat agggtagtat gaaatgattg ttatttatca acccatcgca ggaaatggtc
     4381 gtagagaatg tcagcagctt caccctcact taattcggca gtatttaagc gcaagctggg
     4441 attcataggt gcctcgtagc tgtcggatat cccggtgaag tttggaagcg aaccgttaat
     4501 tgctttgctg tagtttccct tgatatcacg cgactgacaa acctctactg gggcatcggt
     4561 aaatacctca aagaagtttt ttgagccaac aattgacctt gccatttcgc gcattgatat
     4621 cagtggtgaa acgaaacaac agatggttac aactccctgt gaattaagta atgctgccat
     4681 attggccacc ctacgtatgt tctccgttct atcggctggg ctgaatccca aatcgctaca
     4741 taaacccttt cgaacctcat cgccatcgag cagcaccaca ggaattttaa gctgattaat
     4801 acggccttgt agtgctttcc ctagtgttgt ttttcctgaa gcgggtaacc ctgttatcca
     4861 gactgtgata ggatgcttat aactcatgaa aactacctca tatcaataac ttcaacccct
     4921 ttgtgctttt ccacatcaaa tttgaaccga tcgtcacgta aaggaacgtt tgatttgaag
     4981 tttttaattt gaatgatgta gttattgcca tccttaccct ggtaacgaat aaggtatggt
     5041 tcaagggtgt ttgcatcgaa ctgcaactta accgatgagt aaggtaaatt caggtcgcgt
     5101 gggaaaaaat caacctcatg caaaaccctg ttctttgatt tctgctgccc gatatactta
     5161 tacttgaagt tcttgtcgta atccttaaaa agcttcattg gatcgtccaa aaagctattc
     5221 tccttaatat tcatctttga aatggtaacc tcttttgcct ccttaatgta ttgccatttg
     5281 gtttgaccat cggaaaatac ttccatgccc attaggtcga ggtaaaacat tttaccctta
     5341 gccagtatct taccctgatg ggtatcggta atgcgttcct ggaggttttc aagtgtaaag
     5401 ctaaatgttg cggaaagcga tttaatttcc tgcattttgt tactaaaact cctaacaatt
     5461 ccatcggggt ttacctcttg actaaagaga gtaagcgacg atgaaataaa cagcaaactc
     5521 agaatttgtt tcattgataa aagaatttat tcgtgcaaaa atacaaaatt agaaactaat
     5581 ctacggataa agtgttcaaa atacgttcca aagacacctc gtcaacaatt aaaacctgtc
     5641 ggggtttgcc accgtcggca gggccaacaa ttcctgcagc ttccagctga tccataatgc
     5701 gtccggcacg gttataccca atggaaaatt tgcgttggat aagggatgtt gaacccgact
     5761 gggtttgcac cacaaggcga gcggcatcct caaacagttc atcacgtttc cgcaggtcta
     5821 catcaactgg gccatcctga ccctcaggaa catactcggg tagctcatag gcatgaggat
     5881 atccgggctg gttacctata aagtcggtaa tcctctcaat ttcagggata tcaacaaagg
     5941 cacattgcac cctaaccaaa tcgcttccgg cagacactaa catatcgccg cggcctatta
     6001 ggttatttgc acctggcgta tcgagaatgg ttcgtgaatc gaccattgac gacaccctga
     6061 aagcaatacg tgccgggaag tttgccttaa taacacccgt aatgatgttg gttgatggac
     6121 gttgcgtagc aatgattagg tgaataccaa tggcgcgtgc tttttgggca aggcgcccaa
     6181 ggggcatctc aatttcacgc cccgctgtca taattaaatc ggcaaactca tcaataataa
     6241 ccactatgta aggcaaatac ctatggccgt tgttaggatt gagcctgcga gccacaaact
     6301 tctgattata ctcctttatt gttcgaacct tagccaggtt tagaagctta taccgatcct
     6361 ccatctcaac gcttagcgag ttcagggtgt agataacctt ttgggtatcg gtaataatgg
     6421 cctcaggaga atcgggtaac ttggctagga aatggcgttc gagcttgttg tacagggtta
     6481 gctctaccat cttggggtca acaagaacga actttatttc agctggatgc ttcttgtaaa
     6541 gtaacgacgt gataattgcg ttaagcccta ccgatttacc ctgcccggtt gcacctgcaa
     6601 caagcaggtg gggtaacttg gtaagatcca gcataaaaat ctcgttcgaa atggttttac
     6661 ccaatgctat agccaaatcg tatttacact cctggaactt ttgcgacttg ataattgagt
     6721 gcattgacac cacctcgggg ttttggttgg gcacctcaat gccaattgtt ccctttccgg
     6781 gaattggggc aattattcgt attcccagtg ccgaaaggct aagcgcaata tcatcctcta
     6841 aactcttaat cttgctgatc ctaacacctg gggcaggaac aatctcgtaa agagtaacag
     6901 ttgggccaat ggtagcctta atcttatcta tctgaatttt atagtgcgaa agggtttcta
     6961 caatcttgtt cttgttttca accagcattt cattggttac tggcgattca gtattcttgt
     7021 aatcctccag tagttcaatt aatggttttt gatagcttga aagttcaagc gtgggatcgt
     7081 aaagttcctg ttcgttaata tcggatatgg ctagctcttt ctcctcgttt cgctcaatgg
     7141 taaagttatc ctcaatggtt gattctgtta cagttttagt ctccctacca tcgtattcat
     7201 cctgtacgtc caggtcaagt tcatcatcta agtcgtcaat ctcatcagta tcatcaaatt
     7261 catcaacctt ttcttttatc ctttcatttg agatatcaac aggagcaact tcaactattg
     7321 cctgcttaaa atcttcttcg ccatgctttt cggattgaga ttctttttgc ttgcccttaa
     7381 ccacaaaatt tctcaaagaa ccgaaaagaa agaatgtgcc actctgaaag tgataaagcg
     7441 atttaggcat tacgtatata atataggcaa taagtgctat tagaaccaga aaggccgcac
     7501 caaccttacc tatgactgtc gataaccatt ccgagacaaa taaaccatgc gccccgccga
     7561 gtccacttcc taggtatccg cctaattcct tgaataaaaa acctaaagta agcgagccaa
     7621 ccagtatccc cagcaaaacc ttaaaaataa attttgtaac acgtccccta atgaacttaa
     7681 gcagcttaag gcccaaaacc atgaaaatta aagggaatgc caaggctgtt atgccaaacc
     7741 aatgatttat aaactggttt gaaagtgaag cgccaagttt tccaccccag ttgccaatct
     7801 tttgttcagt tgatgagaat attgaaaccc actcaatgct ttgatcaacc ttccaggtga
     7861 acagaaatga taagaacgaa agaaaaatat aaacgcccaa aaggattagg aaaagaccta
     7921 gggtaaacct aatcctttca tccttgaaaa gcgaatcgtt gctaacatcc ttactgtccg
     7981 agtcttcaac atcggtaccc ttatccttcc tagccataat gcataatcaa tataaaacga
     8041 tgtaaaatta acctgtattt gtaaaaaata gaaaccttac tgctgaagta aatagattaa
     8101 atccgtcatg gtttgaaaat caaccttttt gtaatccttt gggattttaa acgcatcatt
     8161 attgatattt tccttggcta cattacgtgc cctgagcttc atttcaagat tattgaacct
     8221 caagttaaaa ccaagcataa ccccatcaat gttttcgaaa ggagtattca ggtttggatt
     8281 gtcaattgca atttgtttgg tgtaaataat ctcaaaccta ttatcagggt tgtcggcaaa
     8341 gtatcccatt acgctcttgc atttatagcc aatataatcg caatctctaa ccaaagtatc
     8401 aatattaacc tttggaattc gagcataaag cgcggggtaa tgcccgttgg tgtatggttc
     8461 actgtggtaa agtttcttgt taaaaacctt tagcaaagta gtaaactcct tttttgatgg
     8521 ttggctaata attgaaattg ttatagcccc tgagagccca tcgatggtgt taatggtgtt
     8581 gttattgcaa aaacgaacaa ccagtgagct gggcaatatt tttttgtcga ttctactgag
     8641 agttaatgta tcgtattcaa tgtcgtattc aattcgtcct tcacttattt cctttgggct
     8701 ttttgatata cagctgctta atgagaataa aatcacagcc acgccaacaa gttggtatac
     8761 acttctgttc atcaacacct tagttcaaat tattaaatca taaaaataga aaatgttttt
     8821 gtggtatgca aaagtttgag gggcgtaata gttatggtaa aaccctgatc agccatcaaa
     8881 catgttatta aacatacata taaaccattg agtgatagtt ttacatcacg tgtattttta
     8941 ttaggattgc aaaaaatagt tagttttgta acttcaaagt aaaaaattta atttactgtt
     9001 tatgagcaaa ttagcagttg tagcattagg cggaaacgcc ctcttgcgtg gaaatcaggt
     9061 gggtaccatc gatgagcaag aacagaatac caccgatacc cttgagaatc tagtttactt
     9121 aataaaggaa gggtataacc ttgtaatcag ccatgggaac ggtccacagg ttggaaatat
     9181 cctcatgcga aacgatgctg gcgagcagct ctatggcatt cctcaaatgc ctcttgatat
     9241 ttgcgttgcc gattcgcagg ggggaattgg ttacatgatt gagcgcatgc ttaggaatgt
     9301 attgcgcaaa catggtattg accgtgaagt atgttgcctg gtaacgccgg taatcgttga
     9361 tcacgacgac cctgccttta ccaaccccac aaaacgtgta ggtcgtattt acgacaaggc
     9421 tcaagccgac gctctggctg ctgaaaaggg ttggatattt aaggaagagg ttaaggatac
     9481 aggaagcggt tggcgcaggg tagttccctc gcctaagccc aaagggattc taaacatcaa
     9541 ggtaatcgat caacttgttc gtcagggtat tattgttatt gcctcaggcg gaggcggtgt
     9601 accggtttat attgacgagg agaagaatgt tagaccagcc gaagcggtaa ttgataagga
     9661 tctggcatcg gcgcttatgg cagccgaaat taaagccgat gaattttaca tcctaaccga
     9721 tgttccattc gtgtatctga actacaaaaa gccgaacgag cagaaacttg agttcctcaa
     9781 ctatgccgat accatgaaat accttgaaca gggaatgttt ggtgagggta acatggctcc
     9841 aaaaattaaa gcatgtttgg actttataaa gcgcggtggc aaaatgagcg tgattaccga
     9901 agccaccaag cttgaggata tgtcatatgg taccaaaatt acaatggagt acgaagatta
     9961 atcaataaac cataaaaaac taaaagctat gtcgttcaac ttacgtaacc gcagcttttt
    10021 aaagctgctc gatttcacac caaaggaaat ccaattcttg ctcgaccttt cagctgatct
    10081 aaaaaaggca aagtatgccg gaac
//
LOCUS       DAOSAL010000081         9739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_4575_length_9739_cov_47.312474, whole genome shotgun sequence.
ACCESSION   DAOSAL010000081 DAOSAL010000000
VERSION     DAOSAL010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 9739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9739
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4575_length_9739_cov_47.312474"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="PK049_09860"
     CDS             complement(<1..713)
                     /locus_tag="PK049_09860"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOK61903.1"
                     /translation="MGNSYFKFKQFTVHQSKAAMRVNTDGVLLGAWANVTGTNRILDI
                     GTGTGVIALMLAQRNTGAHIDAIEIDAQSAAEARSNVNLSPWANRIDVINCSLQSFAP
                     SVSHRYDLIVSNPPYFNQSLKSAHTGRNIARHTESLPYNHLIEGVIKLLAPSGRFCGV
                     FPYSEGNVFIALAGAHGLYCTQKVNVQSRPGRKVLRVLLQLETQKRAVAESTLTIHNP
                     DGSYSDEYKRLTEDFYLGF"
     gene            complement(725..1504)
                     /locus_tag="PK049_09865"
     CDS             complement(725..1504)
                     /locus_tag="PK049_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HOK61904.1"
                     /translation="MLTPKSFAELADLLNDRYRRYACPAFIIGDPIQVPHGFSDKHDI
                     EISALFAATFAWGSRKSIVNSASKLMGIMGNKPYEFVVNFSKADEHALQGFVHRTFSS
                     ADAIEFVYVLRHIYTHLGGLEQVFAKGYRQQQSVHDAIQAYRNVFMECEVPARTLRHV
                     PNVAAGAAAKRINMFLRWMIRPATEGVDFGIWSTIPTSALLMPLDLHTGRVARKLGIL
                     QRAQDDFKAVVELTDALRQFDPADPVKYDYALFGLGLHEKF"
     gene            complement(1507..2157)
                     /locus_tag="PK049_09870"
     CDS             complement(1507..2157)
                     /locus_tag="PK049_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011202004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK61905.1"
                     /translation="MIKVESIVKSFGALTVLKGITVEIPERKVVAIVGPSGAGKTTLL
                     QIMGTLSQPDKGKVLYNGTDVTALNDTQLARFRNSHIGFVFQFHHLLPEFTALENVCM
                     PALIAGKSMTDASNRAKELLDFLGLSHRVSHKPSELSGGEQQRVAVARALINNPMVVL
                     ADEPSGNLDSHNKEELHRLFFHLRDTFGQTFVVVTHDKELARMADLQISMNDGQLV"
     assembly_gap    2162..2261
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2267..2491)
                     /locus_tag="PK049_09875"
     CDS             complement(2267..2491)
                     /locus_tag="PK049_09875"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOK61906.1"
                     /translation="MILLFVGLWELVVIVIVALLLFDSATLLNVARLLGRAYHYLQKS
                     IAEIRSQISLINLDDKDPENDDNPSSKHAG"
     gene            2630..3967
                     /locus_tag="PK049_09880"
     CDS             2630..3967
                     /locus_tag="PK049_09880"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HOK61907.1"
                     /translation="MSKILVLGAGLSSSDLIKYLLDHSKVLGWTVRVGDLSLETARQK
                     VNNHPNGQAIQFDINNNKQLQDEVSWATAVISLLPAFMHPVVAQECVRQGKHMLTASY
                     VSDTMKSLHEEARQKGIALLNELGVDPGIDHMSAMRVIDEIRAKGGKLTGFISNTGGL
                     IAPESDNNPWNYKFTWNPRNVVLAGQGVAQYLENGQYKYTPYHRLFTSVREYNIMDLG
                     MFEMYPNRDSLKYREIYGIEDIQTIIRGTLRRAGYSKAWNVFVQLGMTDDTYKMANSE
                     KLTKREFLNSFLPYHPTETVEEKLKRIIPDAQDEVVFNKLKWLGMFDNEPIGLANASP
                     AQLLQHILEPKLALGPNDKDMIVMQHIFDYELSGKKHRKASTMVIIGKDTIHTAMSIT
                     VGTPLAIATKLLMTGQIKDRGVIVPTKPHVYNPILNELENFGVKFIEEDVELS"
     gene            complement(4074..5501)
                     /locus_tag="PK049_09885"
     CDS             complement(4074..5501)
                     /locus_tag="PK049_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOK61908.1"
                     /translation="MKILLTGATGYIGQRLLPVLLKNGHEVVCCVRDKNRFNIKKYSN
                     QKIKVIEVDFLKKESLIQIPDDIDVAYYLIHSMSTQSGNFQELEVISATNFKERLEQT
                     NVSQVIYLSGISNADELSKHLASRRNVETILSGSKFALTTLRAGIIVGSGSASFEIIR
                     DLVEKLPVMIAPKWLNTRCQPIAITNVIEFLVGVTGRPETYNKAFDIGGPDILSYKDM
                     LLRFARVRGLRRSIYIVPVMTPKISSYWLYFITSTSFSLAQNLVNSMKVEVVCQPNNL
                     TCMLGIKLIDYDTSIRMAFDTIEQNQVISSWKDAQTSNAISKGLGNYIEVPTHGCFKD
                     IRSRKVDSIDAAIKRLWAIGGKNGWYFGNFLWEIRGFIDQLAGGVGMRRGRKSDTDLN
                     PGDALDFWRVLIADKEEMRLLLYAEMKLPGEAWLEFRIDGNGILTQTATFRPLGLLGR
                     LYWYSVLPFHYLIFSRMLKRIAVDQ"
     gene            complement(5498..5968)
                     /locus_tag="PK049_09890"
     CDS             complement(5498..5968)
                     /locus_tag="PK049_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOK61909.1"
                     /translation="MAFYQLIRTQKLPVSTSEIWDFISSPLNLKEITPPYMGFAITGI
                     SGNGKMYEGMIISYRVSPVAGIKTNWVTEITHVKEGSYFVDEQRIGPYAMWHHEHRLE
                     PIEGGVLMTDIVTYQPPLGFIGALANSIFIKKQLQRIFDYRSVALEQRFGKYIP"
     gene            complement(6068..6523)
                     /locus_tag="PK049_09895"
     CDS             complement(6068..6523)
                     /locus_tag="PK049_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61910.1"
                     /translation="MTLYDKYIKVEQAIAKGMNRWSIPAIRISFAIIFIWFGILKPFD
                     VSSAEALLKATVAWLPFGTPEFWLVVIGWWEVAIGVTFLFPKTTKIAIILLFTQMVGT
                     FMPLFFLPQVTFQNGNILTPTMEGQYIIKNVMIISAALVLGGRYYKGKE"
     gene            complement(6556..7254)
                     /locus_tag="PK049_09900"
     CDS             complement(6556..7254)
                     /locus_tag="PK049_09900"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOK61911.1"
                     /translation="MRNYFVVGASSGIGKALSIVLADSGNLVYGTYNKTPVPPETGNI
                     KYYPLDVMDDTINLEFLPEKLDGIAYCPGAITLKPFERIKSTELLSDFQLQVIGAVKL
                     LQAAMPRLRNGNYPSVVLFSTVAVQTGLPYHAVVSVSKGAVEGLTRALAAEYAPKIRV
                     NCIAPSLTDTPLASSLLNNEQKRDSANQRHPLKRVGMTEDIAYMAEFLLSEKSGWITG
                     QVIHVDGGFSTVKL"
     gene            7624..9705
                     /locus_tag="PK049_09905"
     CDS             7624..9705
                     /locus_tag="PK049_09905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HOK61912.1"
                     /translation="MLRCLNTFRYFGIIAIAIIFCGSVRTYAQYYTIQKYSAEGGVKA
                     SGDEQWARAGERLEHPFIVVVTDSSGNPAPGVRVTFEITRVPFGSKGHLLERYEVFTN
                     SQGIARTQLHLGNKEGEYQISASIQTESPQNFVIFTAYANARNWAIMLIVGLLGGLAI
                     FFIGINQMSTGLRHGIADKLRLILLRMSRNRVVATFFGALVTVITQSSSATTVMLVGF
                     VQSGLLRFEQTIGMILGAAIGTTITVQLISFKLSDYSLLIVALGFGIMVFVKKHVTRS
                     IGEALVGFGLLFYGMHLMSGAMSPLKHYEPFLQVIESVQQPLIGVLVGTILTALVLSS
                     AAFIGIVLTMTTQGLISLEGSIPLLMGANLGTAVTGIIAAFAAGREAKKVAMAYAVFK
                     FIGILLLFWLIEPFAQLVRFLTPDVINTFAGGTVTNISRQVANAHTIYNVLLALIVLP
                     FTAQFARIVDWVMPIFPEKQQRYKTQYIDINLINTPSIAIEIARRELVGMAHLVNSML
                     SDSITLFTHKDSRVIDTIREKEEHVNFLRDKLNDYLLRISHADISDNEVDNVFRVMFA
                     TKELEQIGDIISKNLIEKGEWWLGAGLEFSDEGKNELVEFHRLTQKQLNRAIEILDTL
                     NLDVARKQLRKYDHYKKVGIELERNHFDRLKQAVGKSIESSKTHLELVNLYRIIGSHA
                     NNIARITIGEG"
BASE COUNT         2688 a         2166 c         2186 g         2599 t
ORIGIN      
        1 taaaacccca agtaaaaatc ctcggttaac cgtttgtact catcggagta ggaaccatcg
       61 gggttgtgga tggtgagggt gctttcggcc acagcccgtt tttgggtttc gagctggagc
      121 aggaccctta gcacctttcg gccgggtcgc gactgcacgt taaccttttg cgtgcagtaa
      181 aggccatgtg cgccagccag ggcaatgaat acattaccct cggagtaagg gaatactcca
      241 cagaacctgc cgcttggggc aagcagcttt ataacgccct caatcaaatg gttatagggt
      301 aaactctcgg tatggcgcgc aatgtttcgg ccggtgtgag ccgatttgag cgactggttg
      361 aagtatggtg gattggaaac aatcaggtcg taccggtggc ttaccgaggg tgcaaaactc
      421 tgcagcgagc agttgataac atcgatacgg ttagcccagg gcgataggtt cacgttgctc
      481 ctggcctcgg ctgccgattg cgcatcaatc tcaatggcat cgatgtgtgc acctgtgttg
      541 cgttgggcaa gcataagcgc aattacaccg gtacccgtgc caatatcgag tattcggtta
      601 gtgccggtaa cattagccca tgcccctaaa agcacaccat cggtatttac ccgcatggcc
      661 gcttttgact ggtgcactgt gaattgcttg aatttaaagt agctattacc cacggagttg
      721 caagttaaaa cttttcatga aggcctaaac caaaaagggc gtaatcgtac ttaacgggat
      781 cggcgggatc aaactgcctt agcgcgtcgg taagctcaac cacggcttta aaatcgtcct
      841 gcgcgcgttg caggattccc agcttacggg ctaccctgcc ggtatgtagg tcgagcggca
      901 taagtaatgc cgaggttgga atggtactcc agatgccaaa atcgaccccc tcagttgccg
      961 ggcgaatcat ccacctcagg aacatattta tacgcttggc ggcagcaccc gcagcaacat
     1021 tggggacatg gcggagagtg cgtgcaggaa cctcgcattc cataaataca ttgcggtagg
     1081 cctgtatggc atcgtgtacc gattgctgtt ggcggtaacc cttggcaaat acctgttcaa
     1141 gtccgcctaa atgggtgtat atgtgcctaa gtacgtaaac aaactcaatg gcatcggctg
     1201 agctgaaggt tcggtgaaca aagccttgca gggcatgctc gtcggctttg gaaaagttca
     1261 ctacaaactc gtagggctta ttgcccatga tgcccataag cttgctggcg ctattcacta
     1321 tcgattttcg gctgccccat gcaaaggttg cagcaaacaa agcgcttatc tcaatatcgt
     1381 gcttatcaga aaagccatgg ggtacttgaa tgggatcgcc aatgataaat gcagggcagg
     1441 cataccgcct gtacctgtcg ttaagcaaat cggcaagttc agcaaacgac ttaggtgtaa
     1501 gcatggctaa acaagttgac cgtcgttcat ggaaatttgc aggtcggcca tgcgggccaa
     1561 ttccttatcg tgggttacca ccacaaaggt ttgcccaaag gtatcgcgaa ggtgaaagaa
     1621 tagcctgtgc aactcctcct tgttatgtga atcgaggttg cctgagggct cgtcggctaa
     1681 cactaccatg gggttgttga tgagagccct tgcaacagcc acacgctgct gctcccctcc
     1741 cgatagctcc gatggcttat ggctaaccct gtgtgacaaa cccagaaaat caagcagttc
     1801 ctttgcacga tttgaggcgt cggtcatgct tttgcctgca attaacgcag gcatgcatac
     1861 attctcaaga gctgtaaact caggcaaaag gtggtggaac tgaaatacaa agccaatatg
     1921 gctattgcgg aaacgagcaa gctgtgtatc gttaagcgct gtaacatcgg tgccattgta
     1981 tagcacttta cctttatcgg gctggctgag cgttcccata atctggagca atgtggtttt
     2041 acctgccccg cttggcccaa caattgcaac cactttccgt tcaggtatct caaccgtaat
     2101 gcccttgagc acggtaagcg ctccaaacga tttaactatg ctttcaacct ttatcatcta
     2161 gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntcaatctaa ccagcatgct
     2281 tactgctggg gttgtcatcg ttttcagggt ctttgtcgtc caggttgatt aggcttattt
     2341 ggctgcgaat ctcggcgata cttttttgca ggtagtggta ggctcttccc agtaatcggg
     2401 caacatttag cagggttgcc gagtcaaaga gcagcaaagc aacaataaca attacaacca
     2461 gttcccatag gcctacaaaa agcagaatca tggtactcaa cgtttactac ccaaaggtag
     2521 tcagaaaaac aaaaaaagtt tacaaaatca tagtttatca gcctaaaaaa ttatagtttg
     2581 gcaccaaatt taaaccaaaa gctataaacc aaaacctaaa ccttgagata tgagtaagat
     2641 tcttgtacta ggggcaggac tgtcatcgtc cgatttaatc aagtacctgc tggaccactc
     2701 taaagtgctg ggctggactg tacgagttgg cgatttatcg ttggaaaccg cccggcaaaa
     2761 ggtaaataat catcccaatg gtcaagccat acaattcgat atcaacaata acaagcagct
     2821 gcaggatgag gtatcatggg caacggctgt aatatcgttg ctgcctgcat ttatgcatcc
     2881 ggtggttgcc caggagtgcg ttaggcaagg caagcacatg cttactgcct cgtatgtttc
     2941 ggacaccatg aaatcgttgc atgaggaggc ccggcagaaa gggattgccc tgctaaatga
     3001 gcttggggtt gacccgggta ttgaccacat gtcggccatg cgggttattg acgagataag
     3061 ggccaagggt ggaaagctaa ccggattcat aagcaatacc ggcggtctaa ttgcgcctga
     3121 aagtgacaac aacccatgga actacaagtt tacatggaac ccccggaacg ttgttctggc
     3181 tggacaaggg gttgctcagt accttgagaa cggacagtac aagtacaccc cataccaccg
     3241 tctttttact agcgtaaggg agtacaatat catggatttg ggcatgtttg agatgtaccc
     3301 caaccgcgac tcactcaagt acagggagat atatgggatt gaagatatac agaccataat
     3361 acgaggcact ctgcgtcgtg ccgggtatag caaggcatgg aatgtgtttg ttcagcttgg
     3421 catgaccgac gacacctata aaatggctaa cagcgaaaag ctaaccaagc gcgagttttt
     3481 gaatagcttc ctaccctacc atcccaccga gaccgtggag gagaagctga aaaggataat
     3541 ccctgatgcc caagacgagg tggtgtttaa caagctgaaa tggctaggaa tgttcgacaa
     3601 cgaacccatt ggccttgcca atgcaagtcc tgcccaacta cttcagcata tccttgagcc
     3661 caagttagcc cttggcccta acgataagga tatgatagta atgcaacaca tatttgacta
     3721 tgagctttcc ggcaaaaagc atcgcaaagc atcaaccatg gtgattattg ggaaggatac
     3781 cattcacacc gccatgtcta taactgtagg aaccccgcta gccattgcca ctaagttgct
     3841 aatgaccgga cagataaagg atcgtggcgt tatagttcct accaagccgc atgtttacaa
     3901 cccgattctt aatgagcttg aaaactttgg ggttaaattt attgaggagg atgttgagct
     3961 gagctagatt ttaacgatta cctaaaacca taagccggat gttatgtccg gctttttaag
     4021 taattatagc ctaagtagag tatcggaaaa cttttcttaa tactgaaggg aagttattgg
     4081 tcaacagcaa ttcttttaag cattctacta aaaatgaggt aatggaatgg aagcaccgag
     4141 taccagtaaa gcctaccaag caaacccaag ggcctaaagg ttgcagtctg ggtgagtatg
     4201 ccgttaccat caattctaaa ttccaaccat gcctcacccg gtaatttcat ttcggcgtaa
     4261 agtaacaatc gcatctcctc cttatcggca atcaataccc tccagaaatc aagtgcatcg
     4321 cctggattca aatcggtatc gctttttctg cccctacgca taccaacacc gccagcaagc
     4381 tggtcaatga agcccctaat ttcccaaagg aaattaccaa agtaccatcc attttttccc
     4441 cctattgccc acaggcgttt aatggcagcg tcaatactgt caactttacg ggagcggatg
     4501 tccttaaagc atccatgagt gggaacctca atatagttgc ctaatccttt gctaattgca
     4561 ttgctggttt gagcatcctt ccagcttgat attacctggt tctgttcaat ggtatcaaag
     4621 gccatcctta ttgaagtatc ataatcaatt agtttaatgc ccagcataca tgtcaaatta
     4681 ttgggctggc aaaccacttc aactttcata ctattaacca ggttctgagc aaggctaaac
     4741 gaagtggagg taatgaaata caaccagtac gatgaaattt ttggagtcat tacgggaact
     4801 atgtagatgc ttcgtcgtaa accccttacc cttgcaaacc tcaggagcat atccttatag
     4861 ctgagaatat cggggccccc aatatcaaaa gctttgttgt aggtctcggg cctgccggtg
     4921 acacccacta aaaactctat aacattggta attgcaatgg gttggcacct agtattaagc
     4981 cacttgggag caatcataac tggaagtttc tcaactaaat cgcgaatgat ttcaaaggat
     5041 gcactacctg aacccacaat aattccagcc cttaacgtgg taagggcaaa ctttgaaccc
     5101 gatagaatgg tttcaacatt cctacgcgag gctaagtgtt ttgagagctc atcggcatta
     5161 ctgataccac ttaaatagat tacctgactt acattggttt gttccagtcg ttccttaaaa
     5221 ttggtagcac taattacttc cagttcctga aaatttcccg attgcgtgga catggaatgg
     5281 ataaggtaat atgctacatc aatatcatcg ggtatctgaa ttaggctctc ctttttgagg
     5341 aaatcaacct caatcacctt gattttttgg tttgagtatt ttttgatgtt gaaccggttt
     5401 ttatccctta cacagcaaac cacctcatgc ccattcttta gtagaacagg taaaagcctt
     5461 tggcctatgt aaccggtggc acccgtgagt aatattttca tggtatatat tttccaaacc
     5521 tttgctctag tgccacagag cgataatcaa aaattctttg gagctgtttt tttatgaaaa
     5581 ttgagttggc caaagctcca ataaacccca aggggggctg gtaggtaaca atatcggtca
     5641 tcagtacgcc tccttcaatg ggttctaatc tgtgctcgtg atgccacatg gcgtatgggc
     5701 caattctttg ctcgtcaaca aagtaactcc cctcttttac atgggttatc tcggttaccc
     5761 agttcgtttt tattccggcc acagggctta ccctatacga tattatcatt ccctcataca
     5821 tttttccatt accacttatc cctgttatgg cgaaacccat gtagggagga gtaatttcct
     5881 ttaaatttaa gggagatgaa atgaaatccc aaatttcact ggttgaaact ggtagttttt
     5941 gggttctgat tagctgatag aaagccatag cttaaggatt gttgagttta gttaaattgt
     6001 aaacttgcaa ttgtgtcaag cttgaaaaat tattcttcat tttttcccgc tttttcattg
     6061 tatggtacta ctccttgcct ttgtagtatc ggccaccaag cactaatgct gcggaaataa
     6121 tcatcacatt tttaatgatg tattgtccct ccatggttgg agtaagtatg tttccattct
     6181 gaaaggtaac ttgaggtaaa aagaaaagcg gcataaatgt tcctaccatc tgcgtgaaaa
     6241 gtaaaataat agcaattttg gttgttttgg ggaatagaaa agttacacca atggcaacct
     6301 cccaccatcc aataaccacc aaccagaatt cgggtgttcc aaaaggaagc caagccacag
     6361 ttgccttcag caaggcctca gccgacgaca catcaaaggg tttcagtatg ccaaaccata
     6421 taaatattat ggcaaatgag atacgaatag ctggaatact ccacctgttc atacccttgg
     6481 caattgcttg ctctacttta atgtatttat cgtaaagtgt catatttcgt atagtttaat
     6541 tggtaatttt tatgcttata gtttaactgt tgagaaccca ccatcaacat gtattacctg
     6601 gcccgtaatc cagcccgatt tttcggatag cagaaactct gccatgtaag caatatcctc
     6661 agtcattcca accctcttta aagggtgcct ttgattagcc gaatcgcgct tctgctcatt
     6721 gttcaaaagt gatgatgcta agggggtatc ggttaacgat ggggcaatac agtttactct
     6781 aatttttggg gcatattcag cggccaaagc cctggttaac ccctcaaccg caccctttga
     6841 tacggaaaca accgcatggt aaggtaatcc agtctgcacc gcaacagtgg aaaaaagtac
     6901 caccgacggg taattcccat tcctcagcct aggcattgca gcttgaagaa gcttaacagc
     6961 tccaattacc tgtaattgga agtcggataa aagttcggtt gatttaatgc gttcaaacgg
     7021 tttaagcgta atagcgcccg gacagtaagc aatgccatcg agtttttcgg gcaagaattc
     7081 cagattgatt gtatcgtcca tcacatcaag aggataatac ttaatattgc cagtttctgg
     7141 tggaactggt gttttattgt aagttccata aaccaagtta cccgaatcgg ctaaaacaat
     7201 gctaagcgct ttacctatcc ccgacgaagc tccaactaca aaatagtttc tcatattccg
     7261 ttgatttgtt ttgttagtag tataacagat atttaagatt tttgtttaag taatttaaaa
     7321 aatagtatta caaatctcaa aatacaaaaa aagcgcaagg cggagtagtt tataataccc
     7381 tttgtaaagc tgagtaattg tccatgcgtt agcgagaggc acagtgtatt tactccccca
     7441 gaattaatgg gttgattaac attctcatac tgaaaaatct tttttaaacg aaattcgtca
     7501 acttaatttt tcatttggtg aatttgggat tactcattat tagttcaggc attgatttgg
     7561 ggatggtttg aaaaaaaaca ctaaacctat tgccattctc gatatttttg ggtaaattgg
     7621 actatgctaa gatgtttaaa tacattcagg tattttggta taattgccat agctatcata
     7681 ttttgcggta gtgtaagaac ttatgcccag tactacacca ttcagaagta tagtgccgaa
     7741 ggtggtgtaa aagcctcagg cgatgagcag tgggccagag ccggtgagcg acttgaacat
     7801 ccatttatag ttgtggtaac cgattcctca gggaatcctg cccccggggt aagggttacg
     7861 tttgaaatta cccgcgttcc ctttggctca aaaggacacc ttttggaaag gtacgaggta
     7921 ttcaccaaca gtcagggcat tgcacgcacc caacttcact tgggtaataa ggagggcgag
     7981 tatcaaatat cggcaagcat ccagactgag agcccgcaaa actttgtgat ttttacagcc
     8041 tatgccaatg cacgcaattg ggcaataatg ctaattgttg gcttgctggg agggttagca
     8101 atattcttta ttggcataaa ccaaatgagt actggtttac gccacggtat tgccgataag
     8161 ctaaggctaa ttctgctacg catgtcgagg aacagggttg tggcaacctt ttttggggca
     8221 ctggttaccg ttataaccca gtcgagtagc gcaaccacgg tgatgcttgt tggatttgtt
     8281 cagtcgggtt tgttgcgatt tgagcaaacc atagggatga tactgggcgc tgccattggt
     8341 actaccataa ccgttcagct aatctcattc aagttatccg attactcgct gctaattgtt
     8401 gcgcttggct ttggcattat ggtgtttgtg aaaaaacacg ttacccgcag cattggcgaa
     8461 gctcttgtag gctttggcct actgttctac ggcatgcacc ttatgtcggg tgccatgtcg
     8521 ccactaaagc attacgaacc attcctacag gttattgaaa gcgttcagca accattaata
     8581 ggcgtgctgg taggaaccat tttaaccgcc cttgtactga gtagtgcggc atttattggg
     8641 attgtgctaa ccatgaccac ccagggactc atatcgcttg agggttcaat acccttactc
     8701 atgggtgcca acctgggcac ggcagtaacc gggataattg cagcatttgc cgcgggccgc
     8761 gaggccaaaa aggtggccat ggcctacgcg gtattcaaat tcattggaat actccttcta
     8821 ttctggctta ttgagccctt tgctcaactg gttaggttcc tcactcccga tgtaataaac
     8881 acctttgccg ggggaactgt tacaaacata tcgaggcagg tggctaatgc ccacacaatc
     8941 tacaatgtgc tactcgcctt gattgttttg ccttttaccg ctcagtttgc ccgaattgtg
     9001 gattgggtta tgccaatctt ccctgagaaa caacaacggt ataaaaccca gtatatcgat
     9061 atcaacctga taaatactcc atcaattgcc attgagattg cacggcgcga gctggtgggc
     9121 atggctcact tggtaaactc aatgctaagc gatagcatta cacttttcac tcacaaggat
     9181 tcaagagtaa ttgacacaat ccgcgaaaag gaggagcatg taaacttcct gcgcgataag
     9241 cttaacgact acctgctccg aataagtcat gccgatataa gcgataatga ggttgacaat
     9301 gtattcaggg tgatgtttgc caccaaggag ctggagcaga taggcgatat catatcaaag
     9361 aatctgattg agaaaggaga gtggtggctg ggtgcaggac tcgagttttc cgatgaaggg
     9421 aaaaatgagc ttgttgaatt ccataggtta acccaaaagc agcttaaccg agccattgag
     9481 atactggaca cactgaacct ggatgtagcc cgcaagcagc tccgcaagta cgaccactac
     9541 aagaaggttg gtatagagct agaacgtaac catttcgata ggcttaagca ggcggtgggc
     9601 aaatccattg aaagctccaa aacgcacctt gaactggtga acctttaccg cataattggc
     9661 agccacgcca acaacatagc acggattacc attggtgagg gatagaaaaa acagaaccta
     9721 gaatttggga tgggtaacg
//
LOCUS       DAOSAL010000082         9531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_4708_length_9531_cov_46.693225, whole genome shotgun sequence.
ACCESSION   DAOSAL010000082 DAOSAL010000000
VERSION     DAOSAL010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 9531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9531
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4708_length_9531_cov_46.693225"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="PK049_09910"
     CDS             complement(<1..677)
                     /locus_tag="PK049_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOK61913.1"
                     /translation="MATFEVYGGKPLKGELHPQGAKNEALQIICATLLTAEPVTIKNI
                     PNIRDVNKLIELLQGMGVEVQRHSSSECTFRAKNVDVEYLTTNDFKVKAAAIRGSIMV
                     VGPLLARYGRAYAPKPGGDKIGRRRLDTHFLGFQKLGATFNFDTRENFFTLEGKNMRG
                     AYMLLDEASVTGTANILMAAVLTSGTTTIYNAACEPYLQQLSKMLVSMGAKISGIGSN
                     LLTVEGV"
     gene            complement(690..1280)
                     /locus_tag="PK049_09915"
     CDS             complement(690..1280)
                     /locus_tag="PK049_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HOK61914.1"
                     /translation="MDYNTQRKKLRLPEYGRHIQQMVDHIASIEDRDERNRLAKSLIA
                     IMGNLNPQLRAVNDFRHKLWDHLFIMSDFKLDIDSPYPRPEVEKYYEKPRNVPYPSHP
                     VKFKHYGRIIELMIEKAIAMDEGPEKEAFKQLIANQMKKANITWNKESVTDEDIFRDM
                     QTLSNGRLVMAPGSKLIDKREVKPTGGKRPKHHRKR"
     gene            complement(1409..3700)
                     /locus_tag="PK049_09920"
     CDS             complement(1409..3700)
                     /locus_tag="PK049_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOK61915.1"
                     /translation="MTNYLDELSEVQREAVTCTEGPSLVIASAGSGKTRVLTYRIAHL
                     LTLGVKPWSVLALTFTNKAAREMKERIARIVGHETAAKLWMGTFHSIFAKILRNEAEL
                     LGYSKNFTIYDTTDSKNLIAQIIKELKLDVQVYKPGEILSRISIAKNNLITPQAYAQN
                     ASIVSQDISSRKGEIHRIYTLYSQRCQKASAMDFDDLLLNTNILFRDFPDVLQKYQNK
                     FRYILVDEYQDTNFSQYLIVKKLSESHRNLCVVGDDAQSIYSFRGAKIENILNFQNDY
                     KDYKLFKLEQNYRSTQTIVNAANSVIAKNQRQIPKVAYSSNEVGEPIKLIKAFTDQEE
                     GFLVASEIVSLVHQAHCSYSDIAILYRTNAQSRIFEETLRKRNIPYRIYGSLSFYQRK
                     EIKDLIAYIRLTVNPNDDEAFNRVVNYPARGIGDTTMEHLQALANQLGLSLWQTVGEL
                     GKHNGGVKPAAAKKLEEFRNLIVSFQQKVYTMDAYEAVYAIANASGMLTDLKADKSQE
                     GISRLQNIEEMLNGIREFIESRKGEGMEETLTLVDYLENVALLTDQDTDKDGDKNRVS
                     VMTVHSAKGLEFDYVFLTGLEENLFPSSLNIKSQEDMEEERRLFYVALTRAARRAYIT
                     YAQTRYRWGSPTQCTPSRFIKEIDSEYIDVPYSDDEFGSTAERQTVKGQNFGGFSRQP
                     STQSASALSRASTQVPSAPKNAKDISPDAITVGMTVFHAKFGEGVVEAIEGVGPNRKA
                     LVNFNIVGSKNLLLKFANLKQLV"
     gene            3929..4276
                     /locus_tag="PK049_09925"
     CDS             3929..4276
                     /locus_tag="PK049_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61916.1"
                     /translation="MEKLIHSRSTIASLIFNGKMFHLLNPWHIEVDTNQETITIRKRN
                     PFLIGFDEQIVSFRYIRSIMIDEHLFGADIHVKVVGGRASAYCISKSDARRIKNTLLE
                     YNATKRGRGIIFS"
     gene            4368..5759
                     /locus_tag="PK049_09930"
     CDS             4368..5759
                     /locus_tag="PK049_09930"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOK61917.1"
                     /translation="MQSQEKHQESIVVLILVMFTSFITPFIGSAINLALPMIAHEFGL
                     NAVTMSWVTMAFLLSSAVFLVPLGKLADIFGRKRVFLTGNVVLALASLLAVFAPNDAT
                     LIALRVVQGIGSAMMFATGIALITSVFPPNARGRAIGMNVTAVYVGLSAAPVLGGLMI
                     DALGWRSLFYIPALLGIPAVIAAILTIKGEWAEAKGEKFDILGSGIFIVSMCALMYGF
                     SKLPSTVAIVLTAVGLLGLALFVKLQMKLEYPVFNVSLFRVNRLFALSNLAALINYAT
                     TFAITFVLSLYLQYIKGLSAREAGLLLVIQPLVMAVIASWSGRLSDKHDPRILASAGM
                     GVIAVGLALLIPISNETAVTYIGVVLGILGLGFGMFSSPNTNAIMGSVEKRYIGLASG
                     TVATMRLVGQMLSMGIATMVIHVFIGTGTINASNHKQFILSASVTFAIFVALSVLGVW
                     ASLARGGKNGNQA"
     gene            complement(5779..7659)
                     /gene="ispG"
                     /locus_tag="PK049_09935"
     CDS             complement(5779..7659)
                     /gene="ispG"
                     /locus_tag="PK049_09935"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005645004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HOK61918.1"
                     /translation="MRSYIDSIADFKRRVTVEVMVGNTPLGGGNPIRVQTMTTTDTNN
                     TQATVEQCIRAIEAGAEFVRITTQGTREAANLQNIKGELIARGYTTPIVADVHFNPAA
                     ALEAAKHVEKVRINPGNYVDPRAKFETVSYTDQEYADELKRIEERLLPLIEICRERGV
                     AIRIGVNHGSLSDRIMSRYGDTPAGMVESAMEFLRIFRKHGFNQLVISMKSSNTRVMV
                     QAVRLLVATMDAEGMNFPLHLGVTEAGDGEDGRIKSAVGIGALLADGIGDTIRVSLTE
                     EPENEIPVAKKLVNYFANRKTSPSLKSIDSLPYNPYEYNRRVIPEVKGVGGANPPRVF
                     AYINETFTESTANAWGWRFDSVKKLWQPTDAAPDYFVVPGFSPSPNFDGLPVISEHTP
                     NFRLIKVDDFINQKSLGSVMLSVTYADLLNPDLVSALKGSQNVVLLLSTSNSNGFAEQ
                     RAAVIELMRQEICLPVVVARGYSDAEKEDFQLKAAADVGGLLIDGLIDGVMLTSPLSP
                     EVIRNTAFAILQAARTRVTKTEYIACPGCGRTLFSLQQTLQKVRSATSHLKGLKIGVM
                     GCIVNGPGEMADADYGYVGAGPGRITLYKGKEVVKKNIPEEQAIDELIALIKAHGDWV
                     DQ"
     gene            complement(8453..>9531)
                     /locus_tag="PK049_09940"
     CDS             complement(8453..>9531)
                     /locus_tag="PK049_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61919.1"
                     /translation="LGTIIKTNIMTDYPIFLLPKGEIMITAKIEEQELKLLKKTLFPS
                     GNRYAISNNGLAACIDREKKIILYGQINDNGDFDYIKIIPFPSIISPKSLCIIKNNIV
                     LGGENNHVFLNNIISHELVVSYSIPSDKFIVVDMPFKKDNKCVDDLLIDNNKVIAVDD
                     IVYPKYLIEYDFSNPDYPYLIKSYNLPKNGTYERISKGTLNEDYIALLSSSFGMDGGG
                     RYINIFRKGYYDNYIRLSQWFGIKLYGINNRNEKSDKKYFWRDIYLVPNQNILLISSN
                     EDGIGIYRIRNDYMSKQGKEDADSVYYFNSWNKKVIKILTVPNDKEKVLLVFEEGDKE
                     NIKYSYEITSIDDIISNLADDDDY"
BASE COUNT         2536 a         2170 c         2028 g         2797 t
ORIGIN      
        1 tcaaccccct caacggtaag caggtttgaa ccaatacccg atatttttgc ccccatgcta
       61 accagcatct tgctaagctg ctgaaggtat ggttcacatg cagcattgta aatggtggtt
      121 gtgcccgaag taagcaccgc ggccattagt atgttagccg ttccggtaac cgaggcctca
      181 tcgagcagca tgtatgcccc ccgcatgttt ttaccctcaa gggtaaagaa attctcacgt
      241 gtatcaaagt taaaggttgc gcccaacttc tggaatccca ggaaatgggt atccaagcgt
      301 cggcggccaa ttttatcgcc accgggtttt ggggcgtatg ctcggccata tcgcgccagc
      361 agtggtccaa ccaccatgat tgatccgcgt atggctgctg ctttgacctt aaaatcgttt
      421 gtggtaagat actctacatc aacattcttg gccctaaagg tgcattccga ggagctatgc
      481 ctctgaacct ctactcccat tccctgaagc agctcaatta gcttattaac atcgcggatg
      541 tttgggatat tcttgattgt aacaggttca gctgtgagaa gtgtggcgca gatgatttgc
      601 agggcctcat tctttgcccc ctgcgggtgt aactcgcctt ttagcggctt accaccgtat
      661 acctcaaatg ttgccatgta gtaaatattt taacgcttac gatgatgttt gggtcgctta
      721 cctccggtag gtttaacctc gcgcttatca atcagctttg agcccggagc cataaccagc
      781 ctgccattgc tgagggtttg catatcgcga aatatatcct cgtcagtaac cgattcctta
      841 ttccaggtaa tattggcctt tttcatctgg ttggctataa gctgcttaaa ggcttccttt
      901 tcgggaccct cgtccattgc aatggccttc tcaatcatca gctcaataat acgcccgtaa
      961 tgcttgaact taacgggatg gctggggtaa ggaacgttcc gtggcttttc gtaatatttt
     1021 tccacttcgg gcctggggta aggcgaatcg atatcgagct tgaagtcgga catgatgaaa
     1081 aggtgatccc aaagcttatg gcggaaatcg ttaaccgctc tcagctgggg attaagattg
     1141 cccattatgg caataagtga tttggcaagc ctgttacgct catccctatc ctcaatgctt
     1201 gctatgtgat cgaccatttg ctggatatgc cttccgtact cgggtaatcg cagctttttt
     1261 cgttgggtat tgtaatccat ctaaagtgtt ttagtaagaa gagtaaattt aatctgcgct
     1321 cagaaaataa tatgtattta acacaaatat atgtaattgc atgtgaaaaa acaaagtggg
     1381 aataattgcc aaaagtagca caaatgtttt acacaagctg ttttaggttt gcaaatttaa
     1441 gcaataaatt cttagagccc acaatgttaa agttaaccaa agctttgcgg ttaggcccaa
     1501 caccctcaat tgcctccaca acaccctcgc caaacttggc gtggaaaacg gtcattccta
     1561 ccgtaatggc atcgggcgat atatccttgg cgtttttagg agctgatggc acctgagtgc
     1621 ttgcccttga gagcgccgat gcggactgtg tggagggttg gcgactaaat ccgccaaagt
     1681 tttgcccctt cacagtttgc ctctcggccg tgctcccaaa ctcatcgtcg ctatagggca
     1741 catcgatata ttcagagtct atctccttga taaaccggct tggagtgcat tgcgttggcg
     1801 agccccacct atagcgggtt tgggcatagg tgatatatgc ccttctggca gcgcgggtaa
     1861 gcgcaacgta aaaaaggcga cgctcctcct ccatgtcctc ctgtgacttg atatttagcg
     1921 atgatggaaa aaggttctcc tcaagtccag tgagaaaaac gtaatcgaac tcaagcccct
     1981 tggcggagtg aacggtcatt acgctaaccc tgtttttgtc gccatcctta tcggtatcct
     2041 gatcggtaag cagggcaacg ttctcaaggt aatcgaccag ggttagagtt tcctccatgc
     2101 cctccccctt acggctttca ataaactcgc ggataccgtt aagcatctcc tcaatgttct
     2161 gcaggcgcga tatcccttcc tggctcttat cggcctttag gtcggtgagc atgcccgaag
     2221 cgttagcaat ggcatacacg gcctcgtagg catccatggt gtaaaccttt tgctggaacg
     2281 acactatcag attcctgaac tcttcaagct ttttggcagc agcaggttta acccctccat
     2341 tatgtttacc cagttcaccc acggtttgcc aaaggctaag tcccaactgg ttggcaaggg
     2401 cctgaaggtg ctccatggtt gtgtcgccta ttccacgggc ggggtaatta accacccggt
     2461 taaaagcctc atcatcgttg gggtttacgg taaggcgaat gtaggctata aggtccttta
     2521 tctccttacg ttggtaaaac gagaggctac cgtaaatacg atagggtatg ttgcgcttgc
     2581 gcagggtctc ctcaaaaata cgcgactggg cattggttcg gtaaagaatg gcaatatcgc
     2641 tatagctgca atgggcctgg tgcaccaggc tcacaatctc ggaggccaca aggaaaccct
     2701 cctcctgatc ggtaaacgct ttaatcaact ttataggttc gcccacctcg ttactggaat
     2761 aggccacctt tggtatttga cgctggttct tggcaattac gctgtttgca gcatttacaa
     2821 tggtttgggt agaacggtag ttctgctcca gcttgaacag cttatagtcc ttgtaatcgt
     2881 tctggaagtt gaggatattc tcaatttttg caccacggaa cgagtaaatg ctctgggcat
     2941 cgtcgcccac cacgcaaagg ttacggtgac tctcggaaag cttttttaca atcagatact
     3001 gcgagaagtt ggtgtcctga tactcatcaa ccagaatata gcggaactta ttctggtact
     3061 tttgaagcac atcggggaaa tcgcggaaaa ggatattggt gttcagcagc agatcgtcaa
     3121 agtccatagc cgaagccttt tggcatcgct gggagtaaag ggtgtaaatt cgatgtattt
     3181 cacccttgcg ggagctaata tcctgcgaaa cgatggaggc attttgtgcg taggcttgag
     3241 gcgttatcag gttgttcttg gcaatggata ttcgcgacag aatctcgccg ggcttataca
     3301 cctgaacatc gagcttaagc tccttaatga tttgggcaat caggttcttg gagtcggtgg
     3361 tgtcgtaaat ggtgaaattc ttgctatatc caagtaactc ggcctcgtta cgcaggattt
     3421 tagcaaatat tgagtgaaag gttcccatcc agagcttagc agcagtctca tgacccacaa
     3481 tgcgggcaat acgctccttc atctccctgg ctgctttatt ggtaaaagta agcgccaaaa
     3541 ccgaccatgg cttaacgcct agggtaagta agtgggcaat gcgatatgtt aaaacccttg
     3601 ttttacctga acctgccgaa gcaatcacta agctgggccc ctcagtgcaa gtaaccgcct
     3661 cgcgttgaac ctcgcttaac tcatccaaat agtttgtcat tccagctcta aaaaaattta
     3721 ccacaaaagt agtggtagca taccattaca acaagcatat cattagtttg ttatcaacat
     3781 gctttataat gaaacgatat ttattgtcaa attcgatact ggttaatagc gaaactgcaa
     3841 tccaccaaat acatcggcac ggttacatct tgtaaaaatc gattttgttt tatactttta
     3901 gtatgggaaa aaccttttga taaccaaaat ggaaaaatta attcattcac ggtcaaccat
     3961 tgccagtctg atattcaacg gtaaaatgtt ccacctgcta aacccctggc atattgaggt
     4021 agataccaat caggaaacca ttaccatacg taagcgcaac ccattcctga tagggttcga
     4081 tgagcaaata gtatcgttca ggtatatccg cagtataatg atagatgagc acctgtttgg
     4141 cgccgatatt cacgtaaagg tagtaggagg cagagcctcg gcctactgca tttccaagag
     4201 cgatgcaagg agaattaaaa atacgctcct ggaatacaat gccacaaaaa ggggaagggg
     4261 aataatcttt tcatagagca gatatctcgc taaaaaactt aagtttattt tttttgattt
     4321 actttggggc tacaattttg ttgtaattac cccaaggaat cttttatatg caatcgcagg
     4381 aaaaacatca ggaatctata gtagtgctca tcctggtaat gtttacatcg tttattactc
     4441 cctttattgg gtcggcaata aacctggctt tgccaatgat tgcccatgag tttgggctaa
     4501 atgctgttac tatgagctgg gtgaccatgg cctttctgct ctcgtcggca gtatttctgg
     4561 tgcccctagg taagttggcc gacatattcg gacggaaaag agttttcctt accggtaatg
     4621 tagtgcttgc attggcatcg cttttggccg tatttgcgcc caatgacgca acactcatag
     4681 cattgcgggt tgttcaggga attggaagcg ccatgatgtt tgcaactggc atcgccctta
     4741 tcacatcagt tttcccgccc aacgcaaggg gtagagctat tggaatgaac gttactgcgg
     4801 tatatgttgg cctttcggct gcgccagttc ttggtggttt aatgattgac gcactggggt
     4861 ggcgaagttt gttctacatc ccagcattgc tgggcatacc tgccgtgatt gctgctatac
     4921 ttaccattaa aggcgaatgg gccgaagcca aaggcgaaaa gttcgatatt ttagggtcag
     4981 gcatatttat agtatcgatg tgcgccctga tgtatgggtt ctcaaaactc ccatccactg
     5041 ttgctatagt cctaaccgca gtaggccttt taggccttgc cctttttgtt aagctgcaga
     5101 tgaaacttga atacccggtt tttaacgtat cgcttttcag ggtcaatagg ctttttgcgc
     5161 tttcgaacct tgctgcgctg ataaactatg caaccacctt tgccataacc tttgttttaa
     5221 gcctttacct gcaatacatc aagggtttaa gtgcgcgcga ggctggcttg ctactggtta
     5281 ttcaaccgct tgttatggcg gttatagcat cgtggtcggg gcggctttcc gataagcacg
     5341 atccacgcat actggcatcg gcaggcatgg gagtaattgc agtaggtttg gcgctgctta
     5401 ttcctataag taacgaaaca gcagttacct acattggggt ggttctgggc attctggggc
     5461 ttggttttgg aatgttctca tcgcccaata ccaatgccat tatgggctcg gttgaaaaac
     5521 gttacattgg gcttgcatcg gggacggtgg caaccatgcg gttggttggc caaatgttga
     5581 gcatgggtat tgccaccatg gtaatccacg tatttattgg cacgggaacc ataaacgcat
     5641 caaaccataa gcagtttatt ctttcggcca gtgttacgtt tgcaatattt gtagccttaa
     5701 gcgttttagg ggtttgggca tcgctggcaa ggggtggaaa gaacggaaat caagcataaa
     5761 agcatttcaa catcaatttt attggtcaac ccaatcgcca tgcgccttaa ttaatgcaat
     5821 tagctcatca atagcctgtt cctcaggaat attcttcttt acaacctcct tgcccttgta
     5881 aagggtaatc ctgccggggc ctgcacccac atagccgtaa tcggcatcgg ccatttcgcc
     5941 cgggccattt actatacaac ccattacccc aatttttaac cctttcaggt gcgaggtagc
     6001 gctcctaacc ttttgtagcg tttgctgcag gctgaatagc gtgcgtccac agccagggca
     6061 agcaatatat tccgttttgg taactcgtgt tcgggcagcc tgcagaatgg caaatgctgt
     6121 attccttatc acctcagggg atagtggcga agttaacatt accccgtcaa tcagcccatc
     6181 aatcagtagt cctcccacat cggcagcagc tttaagctga aaatcttcct tttcagcatc
     6241 ggagtaaccg cgtgcgacaa ccacaggcaa gcaaatttcc tggcgcatca gctcaatcac
     6301 agcagcacgc tgctcagcaa atccatttga attgctggtg gataacaaca gtaccacgtt
     6361 ttggcttccc tttagtgccg ataccagatc agggtttaac agatcggcat aggtaacgct
     6421 taacatcacc gaacccaatg acttttggtt gataaaatca tcaaccttta tcaagcggaa
     6481 attgggtgta tgttccgaaa taaccggtaa gccatcaaag tttggactgg ggctaaatcc
     6541 gggaactaca aagtagtcgg gtgcggcatc ggttggctgc cataactttt ttaccgaatc
     6601 gaatctccat ccccacgcat tggcagtaga ctcagtaaaa gtttcgttga tgtaagcaaa
     6661 cactcgtgga gggtttgctc ctccaacgcc tttaacctca ggtattaccc gccggttata
     6721 ctcgtacgga ttgtacggta ggctatcgat gctttttaag ctaggcgaag ttttcctatt
     6781 ggcaaagtag tttaccaatt tctttgctac cggtatttcg ttttcgggct cctcggtaag
     6841 tgaaacccga atggtatcgc caataccatc ggcaaggagc gctccaattc ccactgccga
     6901 tttaatgcgg ccatcctcgc catcgccagc ctcggtaaca ccaaggtgaa gcggaaaatt
     6961 cataccctcg gcatccatgg tggcaaccaa aagccttacg gcttgcacca tcacacgggt
     7021 attgctcgat ttcatggaga taaccagttg gttaaaccca tgttttctga aaatacgcag
     7081 gaactccatg gccgactcaa ccattccagc tggagtatcg ccatagcgac tcataatcct
     7141 atcggaaagt gagccatggt ttaccccaat gcgaatggca acgcctcgtt ccctgcaaat
     7201 ctcaattagc ggaagtaacc tctcctcaat gcgtttgagc tcatcggcat actcctggtc
     7261 ggtgtagctt acggtttcaa acttggcgcg gggatccaca tagttgcccg gatttattcg
     7321 caccttttcc acatgcttgg cagcctcaag ggctgccgca gggttaaaat gaacatcggc
     7381 tacaatgggg gtggtatagc ccctggcaat aagttcgccc tttatgtttt gcaggttagc
     7441 agcctcgcgg gtaccctgag tagtaatacg cacaaactcg gctcctgcct caattgccct
     7501 aatgcattgc tcaacggttg cctgcgtgtt gttggtatcg gtagtggtca tggtctgcac
     7561 ccgtatagga ttacctccgc ccagcggagt gttgccaacc ataacctcaa cggttactct
     7621 gcgcttaaaa tcagctatac tatcgatgta gctcctcatg gcaaatagta ttattctgca
     7681 aagtaaatac tttttacagt attacctggg ttgagaagtg atagctgtgc gtaataaaat
     7741 agaacactga ttacactgat ttaattgatt tacacaggat aaattaacca aacaccaaac
     7801 acaatctccc ctgtacactg cacacggtac actgaacact gcacactatt tctcgctacg
     7861 ctcaggatga catgtggggt tgattacctg cgatgatata gagaaaaatg ctcagtggtt
     7921 ggtggttgtt tgttgatagt aaaagcaata aaaaagtaac tatactctgg ttccgtgtca
     7981 tgttgaacgt agtgaaacat ctcaataaaa caacgttcgg gcgcatttac cagacgcgtt
     8041 cgctaaattg gccatgaatg aaatccattt tttcacacgc gacttcatgt tgtttgtgtt
     8101 ttttgggaat tgcatttaag aacgagcagc aactttgata tttataacat gtttatcagt
     8161 tattatgctg gtggtaacgg atacatacat gaaacgtttg gcacttcgaa gcactttcct
     8221 gtcaaattac aagtactttt gatacgaact aaatactcga tatcccactg attaccaaat
     8281 gttttatatg cgatgttaga cactgtaatt atccacttgc ccatgtatca atatcatcta
     8341 tatttccatc aaaataatca taatcaccta actgaccatc cgtcattgcg tcaaaataat
     8401 ctctttccca atcattttct ccagaatcat acctgttatc gtaataatca tcttaataat
     8461 catcgtcatc cgctaaattt gaaataatat catcaatgct agtaatttca tatgagtatt
     8521 ttatattttc tttatcacct tcttcaaaaa caagtaatac tttttcctta tcatttggta
     8581 ctgttaatat tttaataacc tttttattcc atgaattaaa atagtaaaca gagtcagcat
     8641 cttctttacc ttgtttactc atataatcgt ttctaatacg gtatattcca attccatctt
     8701 cgttagatga aatcagtaat atattttgat ttggcacaag gtatatatcc ctccaaaaat
     8761 atttcttatc tgatttttca ttacgattat ttatgccata caattttatg ccaaaccatt
     8821 gtgacaatcg aatatagtta tcataataac cctttctgaa tatatttatg taccttcctc
     8881 caccatccat accaaaagac gaagaaagta aagcgatata gtcttcattt aaagtccctt
     8941 tactaattct ttcataagtc ccattcttag gcaaattata tgactttatc aaatatggat
     9001 aatcaggatt gctaaaatca tattcaataa gatatttggg ataaactata tcatcaactg
     9061 caataacttt attattatca atcaataaat catcgacaca cttattatct tttttgaatg
     9121 gcatatctac aacaataaat ttatcacttg gaatcgaata actaacgact aattcatgag
     9181 agatgatatt gtttaaaaaa acatgattat tttcaccacc taagacaata ttatttttaa
     9241 taatacaaag ggatttaggg cttatgatac ttgggaaagg aataattttt atataatcaa
     9301 aatcaccatt atcattaatt tgtccataaa gaattatttt cttctcacga tcaatacaag
     9361 cagcgagacc attatttgat attgcatatc tattccccga aggaaataaa gttttcttaa
     9421 gcaattttaa ttcttgttcc tcaattttgg ctgtaatcat tatctcgcct ttaggaagta
     9481 agaaaattgg ataatctgtc atgatgtttg tttttattat agtgcccaac g
//
LOCUS       DAOSAL010000083         9071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_5016_length_9071_cov_51.676020, whole genome shotgun sequence.
ACCESSION   DAOSAL010000083 DAOSAL010000000
VERSION     DAOSAL010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 9071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9071
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5016_length_9071_cov_51.676020"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2282)
                     /locus_tag="PK049_09945"
     CDS             complement(<1..2282)
                     /locus_tag="PK049_09945"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HOK61920.1"
                     /translation="MRFRFTLLLLLINQLALLANRFDSVALKQMLAYAKPDTVSTPDS
                     AKNKKSAIDDPVLTNADDSIVYSLDGKIAYLYGNGVVTYQNLELKANYIEFDMETKQV
                     YASGLPDSTGKISGKPKFKEGSQSFEMEKIYYNFDTRRAKIIGVVTEQSGGFMHSAVT
                     KKMEDDVVNLQDGKYTTCDLDHPHFYIAITKGKMIPDKKIVAGPAYLVIEDVPFPLII
                     PFGFFPNTRKRAAGVLIPEYGEENNRGLFLRNGGFYFGMGDYMDQRLTFDVYSRGSWG
                     VRGQTSYKLRYKFSGSLNLEYSTIVTSDKGLPDYSKQNSYWIRWSHNQDPKASPNSTF
                     QANVNFGSSNYNRYNARSINTALSNTFNSSISYSKTWPGSPFSLSTSLTHSQNTINKS
                     VDLNFPRVAFNMSRIYPFKRKNAIGVPKWYEKVGVSLNTSLENRVQVKEDNLFKRTVF
                     DSLQNGMKHSIPVSTSFNLLKFITVSPGVNYNEFWYLQTIEKYWNADSNRVEIDTLRG
                     FKRGYEYSTSVSMSTKVYGMFQFSKKSKVQAIRHVITPSVSLGYRPDFSDPKYGFYRT
                     VQSDSTGKTTRYSIFEKGIYGGPGGGKSGVVSFALSNLLEMKVKSANDTVNPVKKVKI
                     IDGLSMNASYNLLADSLNWSEVSVSGRTGLFDGKVNINYSGGFNLYAINEQGRIYNRY
                     EWNESGKLARFTRGNIGVDFSLNSKKDGQANSSTAGRVGGAGEIPPGTAPDGSNFGES
                     LGTMYGLDYVDFSVPWNVRF"
     gene            2395..3507
                     /locus_tag="PK049_09950"
     CDS             2395..3507
                     /locus_tag="PK049_09950"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOK61921.1"
                     /translation="MALLIISLFSANTTKSQHLSAYSVSKIVIDAGHGGKDPGTVGKI
                     SKEKDITLSIAKRVGELINQNLPGVKVVYTRSDDTFIPLDERSRIANRESADLFISIH
                     CNAAPNKGVTGTETYVMGLHKSNENLDVAMRENAVITYEDEYHEKYEGYDPNSAESFI
                     IFTMLQNAFLEQSLDFASIIQGDLKERAKRPDRGVRQAGFLVLWKTSMPSVLVEVGYL
                     SNPREEMFLNSNEGQELIASSIYRAIKAYKQKIDAIVESNQKTVKPDSSKPENIKNES
                     TTMAKVNTIEFKVQIASSQTPINSKGTQFNGINDIDTLVVNGVYKYLVGGSQSYPEAL
                     EYCKVVKQKFPDAFVVAIENGEIIPIKKALEKINSN"
     gene            3567..4562
                     /locus_tag="PK049_09955"
     CDS             3567..4562
                     /locus_tag="PK049_09955"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOK61922.1"
                     /translation="MKLKLTRETKIGLFALTILVVMFWGVNFLKGKNIFSPNNVYYAI
                     FKSVDGLKNTDAVLINGFKVGLVKNIEFHDLKSGRFRVVLLVNKKYSLPRNTTAKLVS
                     TDIMGGKAIKLEVAPDSLFYQPGDTLPTSIEVGLLDQLAYQMSPVKEKAEVMMSEIAK
                     TLVVLQEVLNDENKKNLSEGIENLNRTLHNIANLSAGLDTMVNSPKGSIRLSLSNIES
                     ISSNIRKNNADITNTIKNLSNLSDSLSKANLYSTLVKLDSSLIQLQQALTKVSSGEGT
                     LGKLVYNDSLYRNMESASLQLELLIKDMKANPKRYVNFSIIDLSRTKYIEEKKNK"
     gene            4897..6333
                     /gene="dnaA"
                     /locus_tag="PK049_09960"
     CDS             4897..6333
                     /gene="dnaA"
                     /locus_tag="PK049_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008774487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOK61923.1"
                     /translation="MAADYKEVWENCLRIIKDNVTPISFKTWFEPIVPIKLEKNVLTI
                     QVPSPFFYEYLEEQYIDILRKTLRKELGADAKLEYSVIMEHSGYNGGTPYTVKFPTQN
                     KTELKNKPVSVQAVQEAPIKNPFIIPGIKKLNVDPQLNPEYNFTNFVEGTCNRLARSA
                     GITISQAPGKTAFNPLFIYGGSGLGKTHLAQAIGIEVKRHFPEKIVLYVNAVKFQTQF
                     VDAYFKHNNINDFLHFYQSIDLLILDDVHEFAGKEKTQDAFFHIFNHLHQTGKQLILT
                     SDQAPADLQGLEKRLLSRFKWGLSAELTTPDFETRVAILKKKVYNDGIVVPNDVLEYI
                     ASNITQNIRELEGALISMLAQSTLNGKQLSVELASEMIEKLVKNTKKDITIDYIQKVV
                     CEYFGLPVDVLNAKTRKREIVQARQIAMYFSKSLTKASLSTIGSVIGNKDHATVLHAC
                     KTVNNLMETDKRFKAQVEDIQKKLKYNA"
     gene            6596..8914
                     /locus_tag="PK049_09965"
     CDS             6596..8914
                     /locus_tag="PK049_09965"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOK61924.1"
                     /translation="MIKIKNLLFALSIATPLMAQSNPDSLIEVIRNNAKDSIILTKIF
                     EKLSPTKEYKDSIYDKVVIEFIKSSASNGWANGVAKGNDLAGNRYYARGNFTKAQEYF
                     QNIIVLSQKTKIPVAIIANAYKMLAGILFNTGNAEGAIEKANNAIDLFQQINDTLSLA
                     KTINLLGGIYWNLGKLEIASEKLFQALRLREKLGDSLGVAHAYNNIGLIYDTQGKQNQ
                     ALDMYYKALTIYQNLKNNLGVGRASNNIAIILKNQKRFTESLDMFLKSYEVDVKNNNV
                     DDQGKTLSNIGQLYLDIGNPKEGINYLLKAKLAFEQSKNENGLAAVFLNLGRAYEQVG
                     NYGTSVAFYARALELAVKLKSIEWQRDAHKGLYSILKRQGNFKKAIEHLEVYKTLSDT
                     LSNISNLNNLDKLKIEYETDKKESEIALLQKDNELKQLDIKRQKVINLLLLSLVVFSL
                     IIVVLSYLYLKKLRKDKRLLQEMNAEILIQKEEIETQRDMLEININELNQHKTELALQ
                     TEQIEMQNRLLEYANHRITEGLEYASFIQRSLLSNPIEFEKYFSKQAMLYKPKDYVGG
                     DIYWHHEFDNGIIFVIADCIGHGVAGAFMSIMAINALKDAVTIFKLNKPSQIATHLYN
                     ELIKNNAKPFDSNLLLGIDFIVCKYTKQAAALEYSGHKISFEYFDSNNNHITVRPQRN
                     LNRQLGLIEFNDEYLDITKKGKLFFYTDGYHDQISDTKRMKLGKSEYIKLLGNTLDKP
                     VNEQVKHLESFLEKWKGNYEQVDDVLVLGIDI"
     gene            complement(8916..>9071)
                     /locus_tag="PK049_09970"
     CDS             complement(8916..>9071)
                     /locus_tag="PK049_09970"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOK61925.1"
                     /translation="SLSSEWDTAAGQAIVEGAGFKVITYETGERLSYNKEDLVNPWFV
                     VSNGRVK"
BASE COUNT         3008 a         1857 c         1718 g         2488 t
ORIGIN      
        1 ctaaacctca cattccaggg aaccgagaaa tcgacatagt caagtccgta cattgttcca
       61 agcgattcgc caaagtttga gccatcggga gccgttcccg gaggtatctc acccgctccc
      121 ccaactcttc ctgcggtgga tgaattggcc tgcccatcct ttttgctatt caatgagaaa
      181 tcgacgccta tattccctct ggtaaacctt gccagcttac cgctctcgtt ccattcatac
      241 ctattgtaaa ttcgcccttg ctcatttatg gcatacaggt taaaaccgcc agaatagttg
      301 atgtttacct tcccatcaaa caggcctgtt ctaccgctaa ccgacacctc agaccagttc
      361 aaagaatcgg ctaaaaggtt atatgaggcg ttcattgaca atccatcaat aatttttact
      421 tttttaaccg ggttaaccgt atcgtttgcc gatttaacct tcatttccag caggtttgaa
      481 agtgcgaaag aaactacccc cgatttgcct ccacctggtc ccccataaat acctttctca
      541 aaaattgaat acctggttgt tttaccagtg gaatcggatt gaactgtcct ataaaaaccg
      601 tatttgggat cagaaaaatc ggggcgatag cctaaggaaa ccgaaggggt aatcacatgc
      661 cttattgctt gcacttttga tttcttagag aactgaaaca ttccataaac cttggtgctc
      721 attgaaaccg atgtgctgta ctcataaccc cttttaaacc cacgcagtgt atcaatctca
      781 accctgttag agtcagcatt ccaatacttc tcaattgttt gcaagtacca aaactcgttg
      841 tagttaactc cggggctaac agttatgaac tttaaaaggt tgaacgaggt agaaacagga
      901 attgaatgtt tcattccatt ttgcagacta tcgaatactg tacgtttaaa tagattatct
      961 tcctttacct gaaccctgtt ttccaggcta gtatttaggc taaccccaac cttctcgtac
     1021 catttgggta ccccaattgc atttttccgt ttaaaagggt aaatcctgct catattaaaa
     1081 gccaccctcg gaaaattcag atcaaccgat ttatttattg tgttttgaga gtgtgtcaac
     1141 gaagttgata agctaaatgg ggagccaggc caggtttttg aatacgaaat acttgagtta
     1201 aacgtatttg agagcgcagt atttatacta cgggcgttat acctgttata gttcgatgaa
     1261 ccaaagttta catttgcctg aaaagttgaa tttggcgaag cttttgggtc ctgattatgt
     1321 gaccaacgaa tccagtacga gttctgttta ctgtaatcgg gtaaaccttt atcgcttgtt
     1381 acaatagtag aatattctaa atttaaagat cctgaaaact tatacctgag tttatatgat
     1441 gtttgccctc gtacacccca cgaccccctt gagtatacat caaaagttaa acgttggtcc
     1501 atgtaatcgc ccatgccgaa gtaaaatcca ccatttctaa gaaatagtcc tctgttattc
     1561 tcctcaccgt attcgggtat taaaacgcca gctgcgcgtt tacgggtgtt agggaaaaaa
     1621 ccaaacggaa taatgagggg aaaaggtaca tcctcaatga caaggtatgc aggaccagca
     1681 acaatcttct tatcgggtat catcttaccc tttgtaattg caatgtaaaa gtgaggatgg
     1741 tctaaatcgc aggtggtata cttaccatcc tgaaggttta ccacatcgtc ctccatcttt
     1801 tttgttactg cgctatgcat gaagccgcct gactgttcag ttaccacacc tataatttta
     1861 gccctacgtg tatcaaagtt gtagtaaatc ttttccattt caaacgactg gcttccctcc
     1921 ttaaactttg gtttaccgct aatttttccg gtactatcgg gtaatccgct tgcgtaaacc
     1981 tgcttggttt ccatgtcaaa ctcaatgtag ttagccttca gctcaagatt ttgataggta
     2041 accacaccat taccatacag atatgcaatt ttaccatcga gggagtatac aattgaatcg
     2101 tcggcgttgg taagtactgg atcgtcgatg gcactttttt tgttcttagc actatcggga
     2161 gttgaaacgg tatcgggttt ggcgtaagcc aacatctgtt ttaaggccac agagtcgaac
     2221 ctgttagcaa gtaatgccaa ctggtttata agtaaaagca gtaaagtaaa cctaaatctc
     2281 aactttaatc ctatttttgt gaacaaattg agctcaaaac tacttaaaaa aataaaagaa
     2341 tacgcaaggg gtatgaggcg ggaatataaa tttttatcaa catttaactt ttatttggca
     2401 ttactaatta tttcactttt ttcagccaat acaactaaat cgcagcacct aagcgcatac
     2461 agtgtatcaa aaattgttat cgatgcgggt catggaggca aggatcctgg aacagttggc
     2521 aaaatcagca aggaaaagga cataaccctt tccattgcca agagggttgg agagctaata
     2581 aaccagaacc tcccgggcgt gaaagtggtt tacacccgat ccgatgatac ctttataccc
     2641 ctagacgaac gtagcagaat tgctaaccgt gaaagcgccg atttgtttat atcaatccat
     2701 tgcaatgctg cacccaataa gggtgttacc ggaaccgaaa cctacgttat ggggttgcat
     2761 aagagcaacg agaaccttga tgtagcgatg cgcgagaatg ctgttattac ctatgaggat
     2821 gaataccacg agaaatatga aggttatgac cctaactccg ccgaatcgtt cataattttt
     2881 accatgcttc aaaatgcttt cctggagcaa agcctcgatt ttgcctcaat tatccaaggt
     2941 gatttaaagg aacgtgctaa gcgtcccgat agaggtgtta ggcaggcagg ctttcttgtg
     3001 ctttggaaaa catcaatgcc aagtgttctt gtggaggttg gctaccttag caatccaaga
     3061 gaggagatgt ttttgaattc aaatgaaggg caagaactta ttgcttcatc aatttacaga
     3121 gccattaaag cctacaagca aaaaattgac gcaattgtag aatcaaacca gaaaacggta
     3181 aagcccgact catcaaaacc agaaaatata aagaatgaat cgacaacaat ggcaaaagta
     3241 aatacaatag agtttaaggt tcaaatagcc tcatcacaaa ccccaatcaa cagtaaagga
     3301 actcagttta atggcataaa tgatattgac acccttgtgg ttaatggagt ttacaaatac
     3361 ttggttgggg gttcgcagtc gtaccctgaa gcactggaat actgcaaggt tgttaaacaa
     3421 aagtttcccg atgcctttgt agtagctatt gaaaacggag agattattcc cattaaaaaa
     3481 gccttagaga agattaactc aaactagatg cctactttcc caataattga gtaattttga
     3541 aatgtaaaca accaaaagaa ccagccatga aactcaaact aacccgcgaa acaaaaattg
     3601 gcctgtttgc ccttaccata cttgttgtaa tgttctgggg agtaaacttt ttaaagggta
     3661 aaaatatttt tagcccaaac aatgtttact acgccatttt caaatcggtt gatgggttaa
     3721 aaaacaccga tgccgtgcta attaacggct ttaaggttgg cctggttaaa aacatagagt
     3781 ttcacgactt aaagtcgggg aggtttcggg tggtattact ggttaataag aagtacagcc
     3841 tcccccgaaa cactactgcc aaactagtaa gcaccgatat aatgggaggt aaggccataa
     3901 agctcgaggt agcccccgat agcctgttct accaacctgg cgacactctt cctacatcca
     3961 ttgaggttgg actcctcgat caacttgcct accaaatgtc gcccgtaaag gaaaaagcag
     4021 aggtaatgat gagcgagatt gctaaaaccc ttgttgtact tcaggaagtg cttaacgatg
     4081 aaaataaaaa gaacctgtcg gagggtattg agaacctcaa tagaaccctc cataacattg
     4141 caaacctgag tgctggcctt gacaccatgg ttaacagccc aaaaggttcc atcaggttat
     4201 cgctaagcaa tatcgagtca atttcatcaa atatccgaaa gaacaatgcg gacattacaa
     4261 acactattaa aaacctttca aacttaagcg attcactatc taaggctaac ctttacagca
     4321 cgcttgtaaa gttagactcc tcgctaatcc agctccaaca agctttaaca aaagtaagtt
     4381 caggtgaggg cacattgggc aaactggttt acaatgactc gctataccgt aacatggaat
     4441 cggcatcatt acagcttgag ttgctgatta aagacatgaa agcgaatcca aaaaggtatg
     4501 ttaatttctc gataatcgac ttaagcagaa ccaaatacat cgaagaaaag aaaaacaaat
     4561 agcagcctat cataaaaatt tgtatatcaa tccgttatac tattccgact gtaagtcaat
     4621 gctatacagt aaatctatat aacctttaaa tttagattca cttaacaata agttaacaat
     4681 caaatatttg tgttattaac aacttatgta actgtatatc agtgcattaa aaaattttga
     4741 cgaacgaaaa aaaaatatta gagtgataat aaggtagttg taatgttaac acaaaaatca
     4801 acctgaaaac ctattttttt ttaaaaaaaa tcattacaat tttgttgatg ttgataacag
     4861 gttgaaaaat tttcactcaa atatttttac atgtatatgg cagctgatta taaagaagtt
     4921 tgggaaaact gtttaagaat cattaaggac aacgtaacac ccattagctt taaaacttgg
     4981 tttgagccca ttgttcccat taagctggaa aagaatgtac tcaccattca ggtgccaagt
     5041 cctttcttct acgagtattt ggaagagcag tacattgaca tactgcgtaa aacgttaagg
     5101 aaggagttgg gagccgatgc taagcttgag tacagtgtaa ttatggagca tagtggctat
     5161 aatggcggca ctccatatac agttaagttc cccactcaga acaaaactga acttaaaaac
     5221 aagcctgttt cagttcaggc cgttcaggaa gcacctatta agaacccttt cattatcccg
     5281 ggaattaaaa agctaaatgt tgatcctcag cttaaccctg agtacaactt cacaaacttt
     5341 gtggagggga catgcaaccg cttagctcgt tctgccggaa tcactattag tcaggctcca
     5401 gggaaaactg catttaatcc attgtttatt tacggaggct cagggttagg caaaacacac
     5461 ctggctcagg caataggaat agaggttaaa agacatttcc ctgaaaaaat tgtactttac
     5521 gtgaacgccg ttaagttcca aacccagttt gtggatgctt actttaaaca taacaacata
     5581 aacgattttc ttcacttcta ccaaagtatc gacttactaa tacttgacga tgttcacgag
     5641 tttgcaggaa aggaaaagac acaggatgcc tttttccaca tctttaacca tctacaccaa
     5701 acgggcaagc agcttatcct tacatcggat caggctccgg ccgacctgca aggcttagaa
     5761 aaacgtctac tatcgcgctt caagtggggt ttgtcggctg agcttacaac acctgacttt
     5821 gaaacgaggg tagcaatact taaaaagaaa gtgtataacg atggtatagt agtgccaaac
     5881 gacgttttgg aatacattgc ctcaaatatc acccagaata ttcgcgagct tgaaggtgca
     5941 ctcatttcca tgctagctca gtcaaccctg aatgggaaac agctcagcgt tgaacttgcc
     6001 tcggaaatga ttgagaagtt agttaaaaac accaagaagg atattactat tgattacatc
     6061 caaaaggttg tttgcgagta ctttggttta cctgttgatg tgttaaatgc aaaaactcgc
     6121 aaacgtgaga tagtacaagc gcgtcagatt gccatgtact tctctaaaag tttaaccaaa
     6181 gcatcccttt caaccattgg tagcgttatt gggaataaag atcatgcaac agttcttcac
     6241 gcctgtaaaa cagttaacaa tctgatggaa accgataaac gattcaaagc ccaggtggaa
     6301 gatatccaaa agaagcttaa gtataacgca taatactagt ttttagggtt tatagtaaag
     6361 gagggagctt acatagcccc tcctttctca ttttcattca acatttaatc aatgaaaatt
     6421 gtaaggttgt caataaagct aaacaaatta aagattaata cgctaaagga attattttta
     6481 ctaccaattt gagttccaag aattgaaatt gtggaaatca tttttcactg gatgattact
     6541 gagaaccata tgtttaaggg aaaaaactga agatataaac tgtaaagcaa tagctatgat
     6601 aaaaattaaa aaccttttgt tcgctctctc cattgcaacc ccactaatgg ctcagtccaa
     6661 ccccgatagc ttaatagaag taatcagaaa caacgcaaag gattctatta ttctaaccaa
     6721 aatatttgaa aaactatcgc caacaaagga gtataaggat tccatttacg acaaggtagt
     6781 aatcgaattc attaagtcat cagcatcaaa tggatgggct aatggagttg ccaaaggcaa
     6841 cgatcttgca ggaaatagat attatgctag aggaaacttt accaaggctc aggagtactt
     6901 tcaaaacatt attgtccttt cacagaaaac aaaaattcct gttgcaatca tagccaacgc
     6961 ctataaaatg cttgcaggaa tccttttcaa tacaggaaat gcagaaggtg ccattgaaaa
     7021 agcgaataat gcaatagacc tattccaaca aattaacgat accctaagcc tggcaaagac
     7081 tataaatttg cttgggggta tctactggaa tcttggaaaa cttgaaatag catccgaaaa
     7141 actattccaa gccctaagac ttagagaaaa actaggtgac tcacttggtg tagcacatgc
     7201 ctataacaac attggcctaa tatacgatac tcagggcaag caaaatcaag cacttgatat
     7261 gtattacaaa gctctaacca tttaccaaaa tctaaaaaat aatttgggtg ttggaagggc
     7321 atcaaataac attgctatca tcctcaaaaa ccagaaaaga tttaccgaga gtctcgacat
     7381 gtttcttaaa tcatacgaag ttgatgtaaa gaacaacaat gttgacgacc agggcaagac
     7441 cctcagtaac atcggacagc tttacctgga tattggaaat ccaaaggaag gcataaacta
     7501 cctattaaag gctaaattgg cttttgagca aagcaagaac gagaatggat tagcagcagt
     7561 tttccttaat cttggtcgcg cctacgagca agtaggaaat tacggcactt cagttgcctt
     7621 ttacgcccgg gctcttgaac ttgctgttaa gcttaaatct attgagtggc aacgcgatgc
     7681 ccataaagga ctgtactcaa tccttaaacg ccaaggtaac tttaaaaaag ccattgagca
     7741 tctggaggtc tataaaacac tcagcgatac gttaagcaat atctcaaatc tgaataatct
     7801 cgataagcta aaaattgagt acgaaacaga taaaaaagaa agtgaaattg ctctgctcca
     7861 aaaggataat gagctgaaac aattagacat taaaagacaa aaggtaatca atttgttgct
     7921 tctatcgctt gttgttttca gtttgataat agttgtgcta tcatacctct acctgaaaaa
     7981 attgagaaag gataaacgat tgctacagga gatgaatgca gagattttaa ttcaaaagga
     8041 ggaaattgaa acacaacgcg atatgctgga aatcaacata aacgaactaa accagcataa
     8101 aaccgaactt gccctgcaaa ccgagcaaat tgagatgcaa aatagactac tggagtatgc
     8161 caaccataga ataaccgaag ggctagagta tgcttcgttt atacaacgtt cgctgctctc
     8221 aaaccccata gagttcgaaa aatatttcag taaacaggcc atgctctaca agccaaagga
     8281 ttatgttggc ggcgatatat actggcatca tgaatttgac aacggaataa tttttgtcat
     8341 tgccgactgt atagggcatg gagtagccgg agcttttatg agtataatgg caattaacgc
     8401 tctaaaagat gcagtaacta tctttaaact aaataaaccc agccaaattg caacgcactt
     8461 gtataatgag ttgataaaaa acaacgcaaa accgtttgat tcaaacctac tattaggcat
     8521 cgactttatt gtttgcaagt acacaaaaca ggcagcagcg ctggaatact cagggcacaa
     8581 aatatcattt gagtatttcg attccaataa taaccatata actgttcgtc cccaacgtaa
     8641 ccttaaccgt cagctcggtt tgattgaatt caacgatgaa taccttgata ttaccaaaaa
     8701 agggaaactc tttttctaca ccgatggtta ccacgaccag ataagtgata ctaagcgcat
     8761 gaagctgggc aaatccgaat acattaaact gctaggtaat accttagata aacctgttaa
     8821 tgaacaggtt aaacatcttg aatcattcct ggaaaaatgg aaaggtaatt acgaacaggt
     8881 tgatgatgta ttggtacttg gaattgacat ttagcctact tcaccctccc attcgaaaca
     8941 acaaaccatg gattaacaag gtcctccttg ttgtacgaaa gtctttcacc cgtttcgtag
     9001 gttatcactt taaaaccagc accctcaaca attgcctgac cggcagccgt gtcccactcg
     9061 ctggaaagcg a
//
LOCUS       DAOSAL010000084         9006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_5062_length_9006_cov_51.127695, whole genome shotgun sequence.
ACCESSION   DAOSAL010000084 DAOSAL010000000
VERSION     DAOSAL010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 9006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9006
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5062_length_9006_cov_51.127695"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="PK049_09975"
     CDS             complement(<1..809)
                     /locus_tag="PK049_09975"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOK61926.1"
                     /translation="MKLYWLILLFLTPIITLKGIAQQPHACRITNTAFVAGERVCYEV
                     SYTWFFIWTDVGEACFTVDSDNRFGKTLLHLKATGKSYPFYDWFFKVRDLYESWVDPV
                     SLSPHYYNRNIYEGGFTKENEYWFNQPGDSVKIRVRRKGGPNKFVSVKSPGCLFDVVT
                     AIYYSRCLNFSGIRQGTVFPVNVLMDEEIFNVKYRYLGKDVRKVTGIGKMACLKFQVD
                     LVAGDIFSTNQKLLVWVTDDANKLPVYIESPIRVGSIQARLKSYQGLRHKLV"
     gene            complement(971..3079)
                     /gene="recG"
                     /locus_tag="PK049_09980"
     CDS             complement(971..3079)
                     /gene="recG"
                     /locus_tag="PK049_09980"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOK61927.1"
                     /translation="MGNILDSEIMYLPGVGPKRAELLQKELNIYTFGDLITFYPFRYI
                     DRTKINKISEVTDELPYVQVVGRVISISEAGVGRHRRLTAIVTDGTGRMELVWFQGIT
                     WVLKRLKPNVDYLIFGKATRFGAHFSIAHPEMEIYNPQQDVITGSLQPVYSTTEKLKN
                     NHITSKTIMGFVQTLIPMAIGKITETLPPFIIEQNKLMTLPEALRNIHFPANTTLLHK
                     AQHRLKFEEHFYIQLSILSRQKVRQTRVNGHVFSHVGEMFNRFYHERLPFPLTEAQKR
                     VIKEIRRDMLSGKQMNRLLQGDVGSGKTVVALMCMLLAVDNGYQACIMAPTEILANQH
                     YESITALMGDLPVKVGLLTGSTRRSERNNLHQSLEAGELNILIGTHALLEDVVKFKNL
                     GFVVIDEQQRFGVMQRAKLWEKNNDPPHVLVMTATPIPRTLAMTLYGDLEVSVIDELP
                     PGRKPVKTLHYTDAKRNLVFALMKEQIKRGRQVYVVYPLIKESEKLENLKDLEDGYES
                     IVRAFPPPEYVTVVVHGQMKPEDKDYSMNLFAQGKAHIMVATTVIEVGVNVPNATVMV
                     IESAERFGLSQLHQLRGRVGRGADESYCILVSQVKLTKESRKRLETMVTTNDGFKIAE
                     VDLHLRGPGDIAGTMQSGMPFDLKMANLATDGQLIQHARQVASEILDHDPLLENPRNS
                     IMKEQLSRLEFHRNDYSGIS"
     gene            complement(3160..3393)
                     /locus_tag="PK049_09985"
     CDS             complement(3160..3393)
                     /locus_tag="PK049_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61928.1"
                     /translation="METLNSSELRQRLYDYSNQVGFDTQKDSFREVISFLIDIDQNFL
                     YTLLNPEEVRYLATHRDVEERLKRQLEQVVESL"
     gene            complement(3569..4876)
                     /locus_tag="PK049_09990"
     CDS             complement(3569..4876)
                     /locus_tag="PK049_09990"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOK61929.1"
                     /translation="MAYSTDSAFLKQLRNTNLGNLIVWSYWWPAIVISAIFLGRIWCM
                     VCPVELITTFFAKIGLKRKRPAWLLSGWAITVFYIIILFIGIQGFAIHRNPFFMAVYL
                     LAIVGVSVIIGSIYEKNTFCRYVCPVGYLLGLYSRLSFFGWRVKDKNICNSCKDKSCI
                     HKKYQYNLNYKSCGVDLYPANITDNSDCILCAGCLKTCSKYQSEPNAERPNPQFTYIG
                     FANGLFQMKPLKMAELIFVLVVSGFVISEIWSEWKITDAYLSFLPDLVVKPLAIENKF
                     GSGFLKGTILFAFVPFILWLLPFLFARLAGVAIKFKAYLVNYGIAFIPIIAAAHLAKA
                     VLKSTSRIPYFEYLFDDFTGVSTAQKIIEGNITLQQTPPWLNTLVSLLLIGFMLIGVW
                     LSIKVVKHLNVKNGKEKSSKSYYLIPILYGGVFLIMLLVWRWF"
     gene            complement(4989..5762)
                     /locus_tag="PK049_09995"
     CDS             complement(4989..5762)
                     /locus_tag="PK049_09995"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOK61930.1"
                     /translation="MEIVIICLAAFFTAVLTFFSGFGLGTILAPVFAIFFPIDIAIAL
                     TGVVHFSNNIFKIALVGKNSDKTVLLRFGIPAILSSFAGAWLLMKITVLPTLLQYELW
                     GRTFEVTPVKLIIAILLIVFSLLEISPSFQKVQFGKDKLIWGGLLSGFFGGLSGIQGA
                     IRSAFLIKIGLSKEVYIATGVVIACLVDFTRLSVYASRFISSTLHENLTLLISATLAA
                     IAGAYFGKRLLKKVTFRSIQLVVAVMLILISIALGAGLI"
     gene            complement(5845..6675)
                     /locus_tag="PK049_10000"
     CDS             complement(5845..6675)
                     /locus_tag="PK049_10000"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOK61931.1"
                     /translation="MNERKIDIKNKLDGGLTFKISRFKEQIKKTKPHKHEDYYELIFL
                     SQGEGFHCIESEKFMVSSPDFYFLKPGQLHYWQFTSIPKGFVILFKDTEFNAVKENVL
                     LDLISKLKDTTRLTFQADKYPSYLLEEIFNEFQINTQYSKEIIHGLLKALFGKLLQIK
                     AENPNSPNQPQPIYERFVQLLVKECPRLHKVNEFADLLNVTPQNLNIICRKQTGKSAS
                     EIITNQLLLEAKRYILHTDNTINEIAEILSFSDASNFVKFFKKHEGITPIQFRDKYFQ
                     "
     gene            complement(7005..8915)
                     /locus_tag="PK049_10005"
     CDS             complement(7005..8915)
                     /locus_tag="PK049_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HOK61932.1"
                     /translation="MGKFEDILKQYWGYTSFRSIQLDIIESVYNGNDTLALMPTGGGK
                     SITFQVPALAMEGICLVISPLIALMKDQVENLKKRGISAVAIYSGMTQHEIDITLDNC
                     IYGEVKFLYVSPERLSTAIFRERIRKMRVCMVAIDEAHCISQWGYDFRPSYLKIAELR
                     SILTEAPFLAVTATATPEVVNDIQEKLHFRNGKVFRMSFARDNLVYLVRNVEDKEKYL
                     FKIVDNIPGSGIVYVRNRNKTKEIALMLQRAGYAADFYHAGLTMEMRNAKQNDWQTGK
                     TRIIVATNAFGMGIDKPDVRFVVHIDLPDSPEAYFQEAGRAGRDQKLSYAVLLYNESD
                     SLRIEQRLEANFPPPDEIKRIYQALGSYLNIAYGAGKGEVYDFNLIDFCSTYKLNMVV
                     AYNSLKILEQEGYIELTDELDNPSRIMFLVNKQELYRYQVAHAEMDKFIKVLLRLLPG
                     VFSQYVRIDEQFLSKTTGLPADKVYENLKTLSRQKILNYIPKKRTPLVIFIEERLDDK
                     NLRIEPERYQTLRERYRARMNAMLQYAQSQAKCRSQFLSEYFGQTDSYRCGKCDVCTA
                     RNELDMSKYEFDNILEKIKATLTKTPLPLENLIDSTGSNPDRVARVIRFLQDTGKITV
                     NCQGLLEWVIRK"
BASE COUNT         2507 a         1881 c         1978 g         2640 t
ORIGIN      
        1 accagtttgt gtcgtagccc ctggtacgat tttagtcgtg cctgtatgct cccaacacgt
       61 atgggcgact caatgtaaac tggtagctta tttgcatcgt cggtgaccca aaccagcaat
      121 ttttggtttg ttgagaagat atcgcctgcc accaaatcga cctgaaattt caggcaggcc
      181 attttcccta tccctgttac tttcctcaca tctttgccta agtacctgta cttgacgtta
      241 aaaatctcct cgtccatcaa tacgtttacc gggaaaacag taccctgcct aatgccggaa
      301 aaatttaaac atcgggagta gtaaatagcg gtaaccacat caaaaaggca accgggcgat
      361 ttaaccgaaa caaatttgtt aggcccgccc ttgcggcgta cccgaatttt tacgctatcg
      421 ccgggttggt tgaaccagta ctcgttttcc ttagtaaatc cgccctcgta gatattgcgg
      481 ttgtagtagt gtggagataa actcacaggg tcaacccacg attcgtaaag gtctcgaacc
      541 ttgaagaacc aatcgtagaa tgggtaactt ttacctgttg ctttaaggtg tagcaatgtt
      601 ttaccaaatc ggttgtcgga atccacagta aaacaggcct cgccaacatc ggtccaaata
      661 aaaaaccagg tgtagcttac ctcgtaacaa accctttcgc cagccacaaa ggccgtgttg
      721 gttattctgc aggcatgcgg ttgctgtgca atgcctttaa gggtaataat tggtgttaaa
      781 aaaagaagga ttaaccagta aagtttcatc cgtacgaaat atttatattg ataattaata
      841 acacgttaca gtcttgcttt taataaactt tatcgagcgg atggaacacc catagtttga
      901 cttcgtcgag ctcgcaaata aacgctcggt tgttcatctg tgtttgtgca agctgcctta
      961 catgggtatt tcatgatatt ccgctgtaat cgttccgatg aaactctagc ctgctgagct
     1021 gttccttcat tatggagtta cgcgggttct ccagtaaggg gtcgtggtca agaatctcgc
     1081 tggctacctg tcgggcgtgt tgaatgagtt gtccgtcggt ggcaaggttg gccattttga
     1141 ggtcgaaagg cattccgctt tgcatagttc cggcaatatc gccggggcca cgaaggtgaa
     1201 ggtcaacttc ggcaatctta aacccatcgt ttgtggtaac catggtttcc agtcttttgc
     1261 gcgattcctt ggttagctta acctgagaaa caagtatgca gtagctttca tcggctcccc
     1321 ggccaacccg tccgcggagc tggtgtagct gcgacagccc aaatcgttca gcgctctcaa
     1381 ttaccattac cgtggcattg ggaacattaa ctcccacctc aatcacggtg gttgccacca
     1441 ttatgtgggc cttgccctgt gcaaagaggt tcatagagta gtccttgtct tcgggcttca
     1501 tttgtccgtg aacaaccacg gttacatact ctggcggtgg aaatgcccga acaatgctct
     1561 cgtacccatc ctcaaggtct ttcaaattct caagcttctc cgattcctta attaacggat
     1621 aaaccacgta aacctgtctg ccccttttaa tctgctcctt cataagcgca aaaacaaggt
     1681 tacgtttggc atcggtgtag tgtaatgttt taacgggttt acgacccgga ggtaactcgt
     1741 caattacgct cacctccaaa tcgccgtaaa gggtcatggc aagcgtgcgg ggaattgggg
     1801 tggctgtcat taccagcaca tggggtgggt cgttgttctt ttcccagagc ttagcgcgct
     1861 gcataacgcc aaagcgttgc tgctcatcaa ttaccacaaa accaaggttc ttgaatttaa
     1921 ccacatcctc cagtagcgca tgggtgccaa taaggatatt gagttcaccc gcctcaagcg
     1981 actgatgcag gttgttgcgt tctgaacgtc tggtactgcc ggttagcaaa cccaccttta
     2041 ccggtaaatc gcccataagt gcagtgattg attcatagtg ctggttggca agtatctcag
     2101 tgggagccat aatgcaggct tggtatccgt tatcaacagc aagcagcata cacatcagcg
     2161 caaccacggt tttgccgcta ccaacatcgc cttgcagcaa cctattcatc tgcttaccgc
     2221 ttagcatatc gcggcgaatc tcctttatta cccgtttctg ggcctcggtt agcggaaacg
     2281 ggagccgttc atggtagaac cggttaaaca tctctcccac atggctgaaa acatgaccgt
     2341 taaccctggt ttggcgaacc ttttgacggc taaggatact gagctgaatg taaaagtgtt
     2401 cctcaaactt aagccggtgc tgcgccttgt ggagcagtgt ggtgtttgcc gggaaatgaa
     2461 tgttgcgtaa ggcttcaggt agggtcatta gcttgttttg ctcaatgatg aagggcggca
     2521 gggtttcggt gatttttcca atcgccattg ggattagggt ttgaacaaat cccattatgg
     2581 ttttggatgt gatgtggttg tttttaagct tttccgttgt ggagtaaacc ggctgtagcg
     2641 acccggtaat aacatcctgc tgaggattat aaatttccat ctcgggatgg gcaatgctaa
     2701 agtgggcgcc aaaccttgtt gccttgccaa atatcagata gtcgacgtta ggttttagcc
     2761 gtttaagaac ccacgttatg ccttggaacc aaacaagttc cattcggcct gtgccgtcgg
     2821 ttacaatggc tgttagccta cggtgccgac ctacgcccgc ctctgagatg ctgataactc
     2881 gcccaacaac ctgtacatag ggtaactcat cggtaacctc cgatattttg ttgatttttg
     2941 ttcggtcaat gtaacggaac gggtaaaagg tgattaaatc gccaaaggtg tatatgttca
     3001 gctccttttg gagcagctcg gcacgcttgg ggccaacacc cggcaagtac attatttcgg
     3061 agtcgagaat gtttcccata cctgaaatgc ttaccacaaa gtaagcaaaa aataaaaaag
     3121 gggaaaactt tccccttaat actattttga cttggattac tatagcgatt ctaccacttg
     3181 ctccagctgc cgtttaagcc tctcctcaac atcgcggtgg gtggccaaat acctaacctc
     3241 ctcggggttt agtagtgtgt ataaaaagtt ttggtcaata tcgataagga atgagattac
     3301 ctcgcggaac gaatcctttt gggtatcgaa accaacctgg tttgagtagt cgtacaggcg
     3361 ttggcgtagt tcagaactat tgagcgtttc cataaaattt tttgtttaca ctcaacctac
     3421 taacaaaagt tgggccaaaa agtttctgga ttactgttta ttttgtaaat aactgtggaa
     3481 tagtaatgta cggttgatat ggaatggttt taccagactc ccttttgttg gttacaaatc
     3541 cgtgtgacca aaaatttaaa acaatcaatt aaaaccatcg ccaaaccaat aacattatca
     3601 gaaaaacacc tccgtaaagt attggtatca gatagtaaga ttttgatgac ttttctttac
     3661 cattcttcac atttaaatgc tttacaacct taatacttaa ccaaactcca ataagcataa
     3721 aaccgattaa aagcaacgag accagtgtat ttaaccaagg tggggtttgt tgtaaggtaa
     3781 tattgccttc aattatcttt tgcgctgtag aaacgcctgt aaaatcatca aagaggtatt
     3841 caaaataagg gattcgtgat gtagatttta aaaccgcttt tgccaaatgg gcggctgcaa
     3901 taatgggaat aaaagcaatg ccgtaattga ccaaatacgc tttgaatttg attgcaacac
     3961 cagccaaccg ggcaaaaaga aatggaagta accataagat gaaaggcacg aacgcaaata
     4021 gtattgtacc ttttaaaaat cccgacccga atttgttttc aattgctaaa ggctttacaa
     4081 ccaaatcggg tagaaaggaa aggtaagcat cggttatttt ccattccgac caaatttcgg
     4141 aaattacaaa ccccgaaaca accaaaacaa aaatcaattc tgccattttc aaaggtttca
     4201 tttgaaaaag gccgttggca aaaccaatgt atgtgaattg tgggttagga cgctcagcgt
     4261 tgggttccga ttgatatttg ctgcacgttt taaggcaacc agcacataaa atacaatcgg
     4321 aattatcagt gatgttggca gggtacaaat cgaccccgca acttttatag tttaaattgt
     4381 actggtattt cttgtggatg cacgatttat ctttacagct attgcaaata ttcttatcct
     4441 taacacgcca accgaaaaat gaaagccttg aatataatcc caacaaataa ccaactgggc
     4501 aaacgtaccg acaaaatgtg tttttctcat aaatacttcc aataattacc gaaaccccaa
     4561 caattgccaa aagatagaca gccataaaaa acgggttgcg gtgaattgca aaaccttgta
     4621 tgccaataaa cagaatgatg atataaaaaa ctgtaatcgc ccaacccgaa agcaaccatg
     4681 cagggcgttt ccgtttaagc cctattttag cgaagaaagt tgtaattaat tctacggggc
     4741 aaaccatgca ccatatacgc cccaaaaata tagcactaat aacaatggct ggccaccagt
     4801 acgaccaaac aattaaatta ccaagatttg tattccgtaa ttgcttgaga aaagcggaat
     4861 cggttgaata cgccattaac cctgttacga ttaatattat atatgcaatg agtgatagaa
     4921 atttcaacga aattggtaaa taccatcttt gtaataaatt attaattatt ttcatttctc
     4981 gttagttttt aaattaatcc agcaccaagg gcaattgata ttaaaatgag cattactgca
     5041 acaacaagtt gaatgcttcg aaaggttacc tttttcagta acctttttcc gaaatatgcc
     5101 cctgcaattg ctgcaagggt tgctgaaatt aaaagtgtaa ggttttcgtg cagggttgat
     5161 gaaataaagc gtgaagcata aaccgaaagc cttgtaaaat ccaccaagca tgctataaca
     5221 acacctgtag caatataaac ttcttttgat agtccgattt ttatgagaaa cgcactgcgt
     5281 attgcaccct gaatgcccga taatccgcca aagaaaccgc taaggagtcc accccatatt
     5341 aatttgtcct taccaaattg aaccttttga aaagaaggtg aaatttcaag taacgaaaat
     5401 acaattaaca atatagcaat tataagtttt actggtgtaa cctcaaaagt cctgccccaa
     5461 agttcgtatt gtaaaagtgt gggtagaact gtaattttca ttaacaacca agctcctgca
     5521 aacgaagata aaatagcagg tattccaaaa cgaagcaaaa cagttttgtc tgaatttttg
     5581 ccgaccaggg ctattttgaa aatgttattt gaaaaatgta ctacaccagt gagagcaata
     5641 gcaatatcaa tagggaagaa tattgcaaaa acgggagcta agatagttcc tagaccgaaa
     5701 cccgaaaaga acgttaaaac ggctgtgaaa aatgccgcca aacaaattat tactatttcc
     5761 atacaaactt ttttctaaat gtatagtgca aaaataggat gtatgattga ctttggattg
     5821 gtaagtttga atgttaatat ggtattattg aaaatattta tccctgaatt gtatgggtgt
     5881 aatgccttcg tgttttttga agaattttac aaagttagaa gcatcggaaa acgataagat
     5941 ttcggctatt tcgttaatcg tattgtcggt gtggagaatg tatctttttg cttctaataa
     6001 gagttggttt gtaatgattt cgcttgcact tttgcctgtc tgtttacggc aaataatatt
     6061 caggttttgg ggtgtaacgt taagcaaatc ggcaaactca ttgactttgt gaagccgtgg
     6121 acattcttta accaataatt gtacgaaccg ctcataaatg ggttggggtt ggttggggct
     6181 attcgggttt tcagccttta tttgcaataa cttcccgaac aatgctttca ataacccgtg
     6241 aatgatttct ttggagtatt gtgtattgat ttgaaactcg ttaaaaatct cctctaataa
     6301 ataagacgga tatttgtcgg cctgaaaagt aagccttgta gtatctttta atttgctgat
     6361 taaatcaagc aatacattct cttttacggc attaaattca gtgtccttga aaagaatcac
     6421 aaatcctttc gggattgagg taaattgcca atagtgaagt tgtccaggtt taagaaaata
     6481 aaaatctggc gaagaaacca taaatttctc cgactcgatg cagtgaaaac cttcaccttg
     6541 cgacagaaat attaactcgt aataatcttc gtgtttgtgg ggcttggttt tcttaatctg
     6601 ttccttgaac cgtgagattt tgaatgtcaa cccaccatca agcttatttt ttatgtctat
     6661 ctttctttca ttcatagcgc aaagttatca aattttaagt ggtgtgattg aaaaactgca
     6721 ctctttgaca ttgctttaat ttgagcgatt gtgtggcttt tacaacgtag ggtatatggg
     6781 tggtttcttt ttttgcgggt ggaaagaaaa acaaattaaa tttggcttcg ccgagttccc
     6841 tttgttcgat tacaaatccg cgcgtgcaac cagaaagttg atggttgttg gttgatggtt
     6901 gatagtgaat gcaatggatt taacaatcgc tttgctggtt tagtgggaat tgcgaataaa
     6961 ttacaaatcc gagcgagtga ggttttttgg gaagaaaaaa gccattactt gcgtattacc
     7021 cattccagta atccctggca gttaacagta attttacctg tgtcctgaag gaaccggatt
     7081 acccttgcaa ctctgtcggg gtttgagccg gttgagtcaa tcagattctc aaggggtagt
     7141 ggtgttttgg ttagtgtggc tttaattttt tccagtatgt tatcgaactc gtacttgctc
     7201 atgtcaagct cattccgggc ggtgcaaacg tcgcatttac cgcatcggta gctgtcggtt
     7261 tggccaaagt actcgcttag gaattggctc ctgcacttgg cctggctttg ggcgtactga
     7321 agcatggcgt tcattcgggc acggtatcgc tccctcagcg tttggtagcg ttcgggttca
     7381 atgcgcaggt ttttatcgtc gagcctctcc tcgataaata ttaccaaggg tgtgcgtttt
     7441 ttggggatgt agttaaggat tttctggcgc gataaggttt tcaggttctc atatacctta
     7501 tcggctggca gtccggtagt tttggaaagg aattgctcat caatccttac gtactgtgaa
     7561 aaaacgcctg gcagcagcct tagcagcacc ttaataaact tatccatttc ggcatgggca
     7621 acctgatacc tgtatagctc ctgtttgttc accaggaaca tgatgcggct tgggttgtcg
     7681 agctcatcgg tgagctcaat gtatccttcc tgttcaagta tttttaggga gttgtatgca
     7741 accaccatgt taagcttgta ggtggagcag aaatcgataa ggttaaaatc gtaaacctcg
     7801 cctttcccgg ctccgtaagc tatgttaagg tacgagccca gggcctggta aatcctttta
     7861 atttcatcgg gtgggggaaa gtttgcctcg agccgttgct caatccttag cgaatcggac
     7921 tcattgtaaa gcaaaaccgc gtaagagagt ttttggtctc tcccggcacg tccggcttcc
     7981 tgaaagtagg cttctgggga atcgggcagg tcaatgtgaa ccacaaagcg cacatcgggt
     8041 ttgtcaattc ccattccaaa agcattggtg gctacaatta tgcgggtttt cccggtttgc
     8101 cagtcgttct gtttggcgtt gcgcatctcc atggtaaggc ctgcgtggta aaaatcggca
     8161 gcataccctg cgcgctggag cattaaagca atttccttgg ttttgtttct gttccgtacg
     8221 taaactatac cgcttccggg tatgttatca actattttga acaggtactt ttctttatcc
     8281 tccacgttac gcaccaggta aaccaggtta tcgcgagcaa agctcatgcg gaaaacctta
     8341 ccgttacgga aatgtagctt ctcctggata tcgtttacaa cctcaggtgt agcagtggct
     8401 gtaactgcca aaaatggggc ctcggttaaa atggaacgaa gttcagcaat tttcaggtac
     8461 gatggacgga aatcgtagcc ccactgggaa atgcagtgcg cctcatcgat agctaccatg
     8521 caaacccgca ttttgcggat tcgttcgcgg aatatggcag tggataatcg ctcgggcgaa
     8581 acgtatagga acttcacttc gccataaatg caattgtcga gggtaatgtc aatctcgtgc
     8641 tgggtcatgc ctgaataaat tgctacagcg cttatgcccc tttttttcag gttttcaacc
     8701 tgatctttca tcaaggctat gaggggtgaa ataaccaagc atatgccttc catagcaagg
     8761 gcgggcacct gaaaggtgat ggatttaccg ccgccggttg gcatcagggc cagggtgtcg
     8821 ttcccattgt aaaccgattc aatgatatcg agctgaatgg aacgaaacga tgtgtagccc
     8881 cagtactgct tgagtatatc ctcgaacttt cccatagaaa caaagatacg aaaaatagtg
     8941 tttggtgttt agtgtttagt ggatagtgtt tggtgtttgg tgtttagtgg atagtgtttg
     9001 gtgttt
//
LOCUS       DAOSAL010000085         9005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_5063_length_9005_cov_51.552737, whole genome shotgun sequence.
ACCESSION   DAOSAL010000085 DAOSAL010000000
VERSION     DAOSAL010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 9005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9005
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5063_length_9005_cov_51.552737"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..470
                     /locus_tag="PK049_10010"
     CDS             6..470
                     /locus_tag="PK049_10010"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HOK61933.1"
                     /translation="MNKYKTIALVAHDNRKRDLVEWVEWNWAKLLKHKLICTGTTGRL
                     VEEALTKMNNDAGNHLQLTRLKSGPLGGDQQLGALIAEGKIDILIFFWDPMQAQPHDV
                     DVKALLRIATVYNIPTATNRSTADFLISSPLIEQEYTPKLKDYSQYINRDVY"
     gene            483..767
                     /locus_tag="PK049_10015"
     CDS             483..767
                     /locus_tag="PK049_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61934.1"
                     /translation="MERNEYRENVKKAIDEIFDQIEALRAKADNASDKVRQEYNEKIK
                     ELEMLRNQMESKLDELAQAADSKWDEVKDTVNSSLESFKEGFKKLGSLFD"
     gene            786..983
                     /locus_tag="PK049_10020"
     CDS             786..983
                     /locus_tag="PK049_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61935.1"
                     /translation="MNAFELSLIVGIVLLLIAWIFVLTDILRNRFPERNMWIIYLIIT
                     PPLAVLVYPIVRERLLRNARK"
     gene            complement(996..1439)
                     /locus_tag="PK049_10025"
     CDS             complement(996..1439)
                     /locus_tag="PK049_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61936.1"
                     /translation="MSEKNYDPRMHTAEHILNQTMVRLYNCGRAFSSHVEKKKSKCDY
                     RIPRALTDEELKTIESRVNEVISQNLNVTEEFTDKSNASTFLDLSKLPADAPESIRVI
                     RVGNYDACACIGPHVANTKEIGRFKIISADYSDGTVRIRFKLENE"
     gene            1575..2468
                     /locus_tag="PK049_10030"
     CDS             1575..2468
                     /locus_tag="PK049_10030"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOK61937.1"
                     /translation="MFDLSKKRWQWAAMLFMAFVWGASFILMKRGLDALSYVQVAALR
                     IFFSFLILLPLSIKNLKLINRQNFWPLLGCGLLGNFFPAFLFTLSETQISSSLAGFLN
                     GLTPFFTVIAGLLLFKHKPNAFQSIGVLIGFVGASILITNGNFSSFGHVNLYALLVVL
                     ATMMYGINSNIIRFKLSGLTGVQITSLIFFLIGIPAGIILISTDIGAAMQHPAFATSI
                     LATIVLATFGSVITLFVYNNLIHHTSAIFASSVTYIIPFFALLWGILDGDHIGALQIV
                     SLVIILIGVYLSSLRRKPKLL"
     gene            2572..3474
                     /gene="ppk2"
                     /locus_tag="PK049_10035"
     CDS             2572..3474
                     /gene="ppk2"
                     /locus_tag="PK049_10035"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HOK61938.1"
                     /translation="MTKDKKDKKKKQADKPKETKKTKKHHLPDLGNDHYETVEAHVDR
                     MDKKTYEHEIEKLHVELVKLLEWIKHQKLKVVLLFEGRDAAGKGGVIKTIAERLNPRF
                     CRIVALGVPTEKEKTQWYFQRYVAHLPAAGEMVLFDRSWYNRAGVEKVMGFCSEEEYT
                     EFLRSCPEFERMLVRSGIILIKYWFSVSDKEQERRFQERIDDPTKRWKLSPMDIESRN
                     RWVEYSKAKDVMFAHTDIKQAPWWVVRADDKRRARLNCIHHLLSLIPYEDLTPPPVKL
                     EPRRIDESYVRPPITDQNFVPEIY"
     gene            complement(3554..3946)
                     /locus_tag="PK049_10040"
     CDS             complement(3554..3946)
                     /locus_tag="PK049_10040"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016271067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOK61939.1"
                     /translation="MDVFKLEFEVRDYECDLQGIVNNARYLNYLEHTRHKYLLSKGID
                     FARLHNEGIDLVVSRIEIDYKYSLTSGDIFVVRLTTHKEGHLRMIFDQEVIKMPEGKL
                     AVKAKVIGVGLKNGRPIKIDSIPGFDNL"
     gene            complement(3959..5221)
                     /locus_tag="PK049_10045"
     CDS             complement(3959..5221)
                     /locus_tag="PK049_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004322272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HOK61940.1"
                     /translation="MNSQKSKGNFLPMLIIGALFFIFGFVTWLNGILIPYFKISCELS
                     DFEATLVAFAFYISYTVMALPASWVLKKTGFHRGMTVGLLVMAIGTLVFVPAALSRAY
                     WMFLSGLFIMGAGLAVLQTAANPYITIIGPRESAARRISIMGICNKLAGAFAPLILAY
                     YILNDGDAFVKSLEGMDSIARAAALDSLAHRVIGPYLVMTSILVFLGIAVRYAPLPDV
                     ETEDDSVDSVTDFTNKSSIFQFPQLILGAIALFFYVGVEVIAGDTIIRYGISQGFKIE
                     TAKVFTTYTLIAMIVGYLAGILLIPKLISQRTALIASALLGIALSIVVVLASPTYSIL
                     ALALLGLANALVWPAIWPLAIHDLGKFIKTGSAVLIMAIAGGAILPLLWGRLSDSISP
                     QMAYIILIPSYFIILYYSVSGYKLRNWK"
     gene            complement(5304..6566)
                     /locus_tag="PK049_10050"
     CDS             complement(5304..6566)
                     /locus_tag="PK049_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HOK61941.1"
                     /translation="MYALADCNNFYASCERVFDPSLIGRPVVVLSNNDGCVIARSNEA
                     KALGIKMGEPAFKIKELIEKANVAVFSSNYTLYGDMSQRVMNTLASFTPDIEIYSIDE
                     AFLGLHGFSYVDLMDYARHIRKTTMRNTGIPISLGLAPTKTLAKVANHIAKKQPQHQG
                     VYMIANEQQRVDALKGFEIGEVWGIGRQYSKFLARYGVNTAYDFTRMPAGWVRQHMSV
                     VGLRTQKELLGIPCIDLEHTAPPKKAIATTRSFGEMQTDLGYLSEAVASFAASCAQKL
                     RKQQSVAQVVMVFAHTNYFRDDLPQYSASKTITLPVPTSSTMELIHYALIGLKSIYKP
                     GFRYKKAGVIVTGISTNQHIQTSLFDSIDREKHSKLMVVVDKLNDKYGRHTVKVAAQG
                     AGRKWKLRQERISPMYTTRWDDIITVKV"
     gene            complement(6569..7009)
                     /gene="umuD"
                     /locus_tag="PK049_10055"
     CDS             complement(6569..7009)
                     /gene="umuD"
                     /locus_tag="PK049_10055"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HOK61942.1"
                     /translation="MRLIKATPKIDFFTADTTSDLSLPLAEGIKAGFPSPAQDYIDLA
                     FDLNKELVKNPSSTFYGRVRGDSMVDEGINDGDILVIDKSLPPTDGRKAVCYIDGEFT
                     LKTIRIKKDGIYLMPANPAYKPIKVTEENDFVVWGIVTYVIHKF"
     gene            complement(7006..7368)
                     /locus_tag="PK049_10060"
     CDS             complement(7006..7368)
                     /locus_tag="PK049_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61943.1"
                     /translation="MDSSSTIKILVDNEKGLSADERTKLIEGTDSIYIDSRLDYHKRL
                     ARRQTVSFVLLILFALFAFGVVMFPSADPFTAGVLKGLVAGYLAALLVLVPKTTKNHS
                     RIAFVISVVKQINSPKQA"
     gene            complement(7474..8289)
                     /locus_tag="PK049_10065"
     CDS             complement(7474..8289)
                     /locus_tag="PK049_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61944.1"
                     /translation="MKPFKKIASYILVISLLASCATPNPVIRLKSKETNTTWESGKEF
                     TSYFKDGFVVHCAYHGTTDAYLVFDIEVINSTDEDFLVCPEKMVMFTDSGKWDPVINQ
                     IVYSTFPIYAADPELEILKNELAQSQIEANQKNLATASIAVAALALPVMVATASADAK
                     DTKQRAVNRAEVAAAAAGTTLNFAEFSQEMNNIRSDLIYASTDAWINNSLRKTTLSKN
                     EAVRGLVYFKKPDLKMYRDLRIDVPIGENNFISFNYKVNLYYPSNPNVNPAYR"
BASE COUNT         2601 a         2023 c         1894 g         2487 t
ORIGIN      
        1 taaaagtgaa taagtacaaa accatagccc tagtagcaca cgataaccgc aaacgcgacc
       61 ttgtggaatg ggttgagtgg aactgggcaa aattactaaa gcacaagctc atttgcacag
      121 gcacaaccgg ccgtcttgtt gaggaggccc taaccaagat gaataacgat gccgggaatc
      181 atctgcagct tactcgcctt aaatcgggtc cacttggtgg cgaccagcaa ctaggcgctt
      241 taatagcgga agggaagatt gacatactaa tctttttctg ggatcccatg caggctcagc
      301 ctcacgatgt ggatgttaag gctttgcttc gcattgctac ggtttacaat attcccacag
      361 ccaccaaccg ttcaacagcc gacttcctaa taagttcgcc acttattgaa caggagtaca
      421 ccccaaaact aaaggattac agccagtaca taaacagaga cgtttactaa aaatattgag
      481 ccatggaacg taacgagtac cgcgaaaatg taaaaaaagc cattgacgaa attttcgacc
      541 agattgaagc attgagggct aaagccgata atgcctccga taaggtaagg caggagtaca
      601 atgaaaaaat caaggagctt gaaatgcttc gtaatcaaat ggaaagcaag ctcgatgaac
      661 ttgcccaggc agccgatagc aagtgggacg aggtgaaaga tactgtaaat tccagccttg
      721 aatcgttcaa ggagggcttc aagaagttag gttcgctttt cgattagcca atacttcact
      781 tacaaatgaa tgcatttgaa ctttccctaa ttgtagggat tgtactacta ctaattgctt
      841 ggatttttgt tcttaccgat atccttcgta acagattccc tgaacggaat atgtggatta
      901 tttacttaat cattacccct cccctggctg tgttagtata tcctatagtt cgggaaagac
      961 ttttaagaaa tgcaagaaag tagtgtgagt tgaaattact cattttcaag cttaaacctt
     1021 atccgtacag ttccatcgga gtaatcggcc gagataattt tgaatctccc tatttccttg
     1081 gtgttagcca cgtgcggacc aatacaggcg caagcatcgt agtttcctac cctaataacc
     1141 ctgattgatt ccggggcatc agcgggtaat ttgcttaaat caaggaaagt tgacgcattg
     1201 cttttatcgg taaactcctc ggttacattt aggttttgtg agataacttc attcacccta
     1261 ctctcaattg tttttagttc ctcatcagtc agagctcttg ggatgcgata gtcgcacttg
     1321 gacttttttt tctccacatg gctactgaac gccctaccac aattgtaaag ccgcaccatt
     1381 gtttggttga gaatatgttc ggcggtatgc atacggggat cgtagttctt ttcgctcatt
     1441 tcagtttaaa ttttcatgta aaaatagtaa aaagggaaag caatattagc agaaacaaat
     1501 aggctgtaaa tgttgacagg aaaaagcacc catcaggcca aaaacaatta cttttgccgg
     1561 ataaaacttg tcaaatgttc gatttatcca aaaaacgctg gcagtgggct gccatgcttt
     1621 tcatggcttt tgtttgggga gcctcgttta tcctgatgaa gcgcggtctc gatgcgctaa
     1681 gctacgttca ggtggctgcg ctccgaatat ttttcagctt tttgatactt ctaccccttt
     1741 caataaaaaa cctaaagctt ataaaccgcc agaatttttg gccgctacta ggatgcggat
     1801 tactgggtaa ctttttccca gcattccttt ttacactttc tgaaacccag atctccagct
     1861 cgcttgcagg attcctcaac gggcttaccc ctttctttac ggtaattgcg ggtttgttgt
     1921 tattcaaaca taagcccaat gctttccaaa gtataggcgt tctaatcgga tttgttgggg
     1981 catcaatact aataaccaac ggaaattttt cgtcgtttgg ccatgtaaac ctttatgcac
     2041 tgctagttgt gctggccact atgatgtatg gcattaattc caatattatt cggtttaaac
     2101 tatcggggtt aaccggagtg caaattacat cgctaatctt tttcctgatt ggcattccgg
     2161 ccggtattat acttatatca acagatattg gggctgccat gcagcaccca gcatttgcaa
     2221 cgagcattct ggctaccatt gtgctagcaa catttggctc agtaattact ctatttgtgt
     2281 ataataatct tattcaccac accagcgcaa tttttgcttc atcggtaacc tacattattc
     2341 ctttttttgc gttactatgg ggcattcttg atggcgacca tattggagca ctacaaattg
     2401 tctcgttggt aataatcctg attggggttt acctctcaag cttacgccgc aagccaaaat
     2461 tgctttaaaa tcaggtcatt ttaataaaat taaatcaatt ttatccaaaa agggttgact
     2521 ttacccttat ttgtccatac ctttatttta aagtcaaaaa ccttgtttga catgacaaag
     2581 gacaaaaagg ataaaaaaaa gaaacaggcc gataaaccaa aagaaacaaa aaaaaccaaa
     2641 aagcatcacc tgcctgattt aggaaacgac cattacgaaa cggtagaggc ccatgttgat
     2701 aggatggata aaaagaccta tgagcatgaa attgaaaaac tccatgtgga actggtaaaa
     2761 ctccttgaat ggataaagca ccaaaaattg aaggttgttt tactgttcga ggggcgcgac
     2821 gctgccggaa agggcggtgt aattaaaacc atagccgaac ggctaaaccc tcgtttttgc
     2881 cggattgttg ctctaggtgt acctaccgag aaagagaaaa cccaatggta tttccagcga
     2941 tatgtagccc acctacccgc tgccggtgag atggttctgt ttgaccgaag ctggtataac
     3001 cgggctggcg ttgaaaaggt catgggattt tgttctgagg aggagtacac tgagtttcta
     3061 cgctcatgcc ctgagtttga acgtatgctg gttcgttcgg gtataatact aattaaatac
     3121 tggttctccg ttagcgacaa ggaacaggaa cgtcgctttc aggagcgtat cgacgatcca
     3181 accaagcgat ggaagctcag ccccatggat attgaatcgc gtaaccgctg ggtggaatac
     3241 tccaaggcaa aggatgttat gttcgcccat accgatataa agcaagctcc ctggtgggta
     3301 gttcgagccg acgataaaag gcgtgcccgc cttaactgta tccatcacct gctaagcctc
     3361 ataccttacg aagatcttac cccaccaccg gttaagcttg aaccccgacg gattgacgag
     3421 agctatgttc gtccacctat caccgaccag aactttgttc ccgaaattta ttaattgcaa
     3481 atagggctgc tcgcatgggc agcccaaaat gtttaaaatc gccaataaaa aaaaatcaat
     3541 tgataaatcg cggttaaagg ttgtcaaagc ccggtatact atcaatcttg attggacggc
     3601 cattttttag cccaacgcca attaccttag ccttaacggc taatttcccc tcgggcattt
     3661 taatcacttc ctgatcaaag atcattcgta aatggccctc cttgtgggtg gttaaccgaa
     3721 caacaaatat atcgccactg gttagcgagt acttgtagtc aatttcaata cgtgacacca
     3781 caaggtcaat gccctcattg tgcaacctgg caaaatcgat tcccttgctc aacaagtact
     3841 tgtgacgggt atgctcaagg tagttcaggt agcgagcatt gttaacaata ccctgcaagt
     3901 cgcattcgta atcacgaacc tcaaactcta gcttaaaaac atccataatt tttacgtttt
     3961 acttccagtt ccttaactta tatccgctca cagagtagta aagaattata aagtacgatg
     4021 gtatcagaat aatgtatgcc atttggggcg aaattgaatc ggatagtcgg ccccaaagca
     4081 agggcagaat ggcaccaccc gcaatggcca tgataagcac cgccgaaccc gttttaataa
     4141 atttgcctaa atcgtgaatg gccaatggcc agatggcagg ccaaaccaat gcatttgcta
     4201 aaccaagcag tgcaagtgca agaattgagt aagttggcga tgccaaaact acgacaatac
     4261 ttagagcgat acccagcaaa gcagatgcaa taagagccgt gcgctgcgaa ataagttttg
     4321 gtatcagtaa tattccagcc aggtatccaa ctatcatggc aataagggta tatgttgtaa
     4381 aaacctttgc ggtttcaatt ttaaaacctt gcgatattcc atagcgaatt atggtatcgc
     4441 ctgctattac ctcaacacca acatagaaaa acagggcaat agcaccaagt atcagctgcg
     4501 ggaactggaa aatacttgat ttattagtga aatcagtaac agaatctacg gaatcatcct
     4561 ctgtttccac gtcgggcagc ggggcataac gtacggcaat ccccaggaaa accagtattg
     4621 atgtcattac caggtacggc ccaatcactc tgtgcgctag gctatcgaga gccgcagcgc
     4681 gggcaatact gtccatcccc tcaagtgatt tcacaaaggc atcgccatcg ttaaggatgt
     4741 agtaggccag tataagcgga gcaaaggctc cagctaactt attacaaatc cccattatac
     4801 ttatacgcct tgcagcactc tcgcggggtc ctattatggt gatatacgga tttgcagctg
     4861 tttgcagcac agccaaccct gctcccataa taaacaggcc actcagaaac atccagtaag
     4921 ctctgctcaa cgctgcggga acaaacacaa gggttcctat agccatcacc agtaaaccta
     4981 ctgtcatgcc cctgtgaaaa cctgtctttt tcaacaccca cgatgccggc aaagccatta
     5041 cggtatagga gatataaaag gcaaaagcta ccagggtagc ttcaaaatcg ctaagctcac
     5101 acgaaatttt aaaatacgga ataaggatgc cgtttaacca ggttacaaaa ccaaaaatga
     5161 agaataacgc tccaataatg agcataggca aaaaattgcc tttacttttt tgagagttca
     5221 ttgcagttgt tttaggttaa ggtaccccaa aaatagaaaa atattgatga gcgaagaggc
     5281 caaatcaaaa taaaaaagga aacttaaacc tttacagtaa taatatcgtc ccagcgagtg
     5341 gtgtacatgg gcgaaatacg ttcctggcgt aatttccatt tacgtccagc cccctgagcg
     5401 gctactttaa ccgtgtgcct gccatactta tcgtttagtt tatccacaac caccattagt
     5461 ttactgtgtt tttccctgtc aatgctatcg aagagcgagg tttgaatatg ctggttggtt
     5521 gaaatacccg ttacaattac tcccgccttc ttgtaccgga atcccggctt gtatatactt
     5581 tttaatccaa taagggcgta gtgtatcagc tccatggtgc tgctcgtggg cacaggcagg
     5641 gttatggttt tacttgccga gtactggggt aaatcgtcgc gaaaatagtt ggtgtgtgca
     5701 aaaaccatta ccacttgcgc aaccgactgc tgcttacgca atttctgggc gcaactcgct
     5761 gcaaacgatg ccaccgcctc actcaggtat ccaaggtcag tttgcatctc accaaaggaa
     5821 cgggttgttg caattgcctt tttgggtgga gcggtatgtt ccaaatcaat acagggtatt
     5881 cccagtaact ccttttgggt tcggagtcca accactgaca tgtgctgcct tacccaacct
     5941 gcgggcattc gggtaaaatc gtatgctgta ttaacaccat agcgggccag aaacttactg
     6001 tactgccggc ctattcccca aacctcacca atctcgaacc cttttagcgc gtcaacacgc
     6061 tgctgctcgt tggcaatcat ataaactccc tggtgttgtg gctgcttttt tgcgatatgg
     6121 ttagcaacct ttgctaacgt tttggtaggg gctaagccca agcttatggg aataccggta
     6181 ttccgcatgg tggttttccg aatatgtcgg gcataatcca ttaaatcaac atagctgaaa
     6241 ccgtgcagcc caagaaaagc ctcatcaatg gaatatattt cgatatcggg ggtaaagctg
     6301 gcaagggtat tcatcaccct ttggctcata tcgccataaa gcgtatagtt tgatgagaag
     6361 acagcaacat tggctttttc aatcagttcc ttaatcttga aggctggctc acccattttg
     6421 ataccaagag cttttgcctc gttgctgcgg gcaattacac aaccatcgtt gttcgacaga
     6481 acaacaacag gcctcccaat gagcgaaggg tcaaaaacac gctcgcacga agcgtaaaag
     6541 ttattgcaat cggcaagagc gtacatggct aaaacttatg gatgacgtag gttacaatac
     6601 cccataccac aaagtcgttt tcctcagtaa cctttatggg tttatatgcc ggatttgccg
     6661 gcataaggta gattccatcc tttttaatcc gaatggtttt aagcgtaaac tcaccatcga
     6721 tatagcaaac tgctttacgc ccatcggtgg gtggtaacga tttgtcgata accagaatat
     6781 cgccatcgtt aatcccctca tcaaccattg aatcgccacg gacacgacca taaaaagttg
     6841 aacttggatt cttgacaagt tccttgttta aatcaaaggc taaatcgata tagtcctgcg
     6901 ctggcgacgg aaaaccagcc tttattcctt cggccaaagg aagtgaaagg tcggatgttg
     6961 tgtcggcagt aaagaaatct atcttaggtg tagcttttat cagcctcatg cttgtttagg
     7021 ggagttaatc tgtttaacta cactaataac aaatgcaatt cgcgaatggt ttttagttgt
     7081 tttaggaaca agaacaagga gtgcagcaag ataccctgca accaatcctt ttagtactcc
     7141 tgcagtaaaa ggatcggcgc taggaaacat taccacgcca aaggcaaaaa gtgcaaaaag
     7201 tatcagcaaa acaaagctta cagtttgcct tcgggctaac cgcttgtggt aatcgagcct
     7261 tgaatcaatg taaatactat cagtgccctc aattagtttt gtacgttcat cggccgacaa
     7321 tcccttttcg ttatcaacca atatcttaat tgttgaactg ctgtccataa cattcagatt
     7381 ttttacaaat ttactacatt aaaccatgaa cttatcagga atactaaaaa gaaaaaaggc
     7441 cctcaggcct tttcccttga ttgttaagca aatctaccta tatgcgggat taacattcgg
     7501 atttgagggg tagtacaggt ttaccttgta gttaaaggat atgaaattat tctcgccaat
     7561 gggaacatca atgcgtaaat ccctgtacat tttcagatcg ggctttttaa agtaaactag
     7621 cccacggact gcctcgtttt tagatagggt tgttttgcgg agtgagttat tgatccatgc
     7681 atctgttgat gcgtaaatca gatcgcttcg gatattattc atctcctggg agaattcggc
     7741 aaagttaagg gttgtccctg ccgctgctgc agccacctcg gctcggttaa ctgcacgctg
     7801 tttggtatcc ttggcatcgg ctgaggctgt tgcaaccatt accggtaatg ccaatgcagc
     7861 tactgcaatt gaagcagtag caaggttttt ctgattagcc tcaatctgcg attgcgccaa
     7921 ttcattttta agaatttcta gttcgggatc agcggcatag attgggaatg tgctgtacac
     7981 aatctggttg attaccggat cccacttccc actatcggta aacatcacca ttttttcggg
     8041 gcaaaccaga aaatcctcat cggtagagtt aatcacctct atatcgaaca ccaagtaggc
     8101 atctgtagta ccatggtaag cgcaatgaac cacaaagcca tccttaaagt aactggtaaa
     8161 ttctttccca ctttcccagg ttgtatttgt ttccttagat tttaacctga taactgggtt
     8221 tggagttgca caagaagcca ataagcttat tacaaggatg taactggcta tctttttaaa
     8281 aggtttcata gattttaggt agttggttta acgtaagccg tcaaataaca tgccatgaac
     8341 ctaaaaaaat caagcattct catacattaa tttgatgttg aaaaacacct atggaacaat
     8401 ttaaatgaaa gatcagccca gttgggttaa aaacgagatt cctcgctgtg ttcgggatga
     8461 cggtttacga tattcgtgaa tcgctgaaca ttgttcgtaa cacctttgtc atgttgagcg
     8521 gtagcgaaac atctctttag ggccgattag agttccttcg ctccccgcta gggcgggaca
     8581 ggccgctcag gatgacaagc ggagttgaca gttgatagtt gatagtgaaa aaaagcattt
     8641 cacgaaacat ctaactccaa acacccctat acactgtaca ctgtacactt ttcaatagat
     8701 cattcgctac gctcaggatg acaaatgaag ttgttcgtgt aagttgttcg ttgctcgtga
     8761 aactattgtc atgctgagct agccgaagca tctcctttcc accttttatg agatccctcg
     8821 ctttgctcgg gatgacaaaa accctatctt ccttttctgc tcccttgtca tgctgagcta
     8881 ggcgaagcat ctctttacct tcaactcttc ttttttaatg cgattcctta accaacacgg
     8941 agtgccatac gcagaggcat aaaagcctga cccggcaatg ggtcgggggt ggaacgtggc
     9001 ggtgg
//
LOCUS       DAOSAL010000086         8838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_5169_length_8838_cov_51.073323, whole genome shotgun sequence.
ACCESSION   DAOSAL010000086 DAOSAL010000000
VERSION     DAOSAL010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 8838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8838
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5169_length_8838_cov_51.073323"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..435)
                     /locus_tag="PK049_10070"
     CDS             complement(82..435)
                     /locus_tag="PK049_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HOK61945.1"
                     /translation="MNIELIREHCISKPDVTESFPFDDDTLVFKVNGKIFAMVNLSGD
                     TQLNLKCDPELAIELREKYMAVQPGYHMNKRHWNTIAIDGSIPDALILDWIDHSYNCV
                     CLNKGKRKLGSRKKK"
     gene            complement(456..833)
                     /locus_tag="PK049_10075"
     CDS             complement(456..833)
                     /locus_tag="PK049_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007559781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOK61946.1"
                     /translation="MVEKIGGNKDLGSKGEEIAASYLAAKGYKILHRNWHFGHKELDL
                     VTQLNDTLVIVEVKTRSTHYWEEPKESVKRKKQRMIVEAADEYVQRHNLDCDVRFDIV
                     SIVINHGLPPEIEHIEDAFFPTL"
     gene            897..1535
                     /locus_tag="PK049_10080"
     CDS             897..1535
                     /locus_tag="PK049_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61947.1"
                     /translation="MRFICYIAFFTLLLSTNAYSKVGGFEGKVNIVREGVYDTIQVEV
                     YIKGNLIRVNEFSKGGKLIRSYFANVESGSTYLVSFSNRKYSKVKVDNSPAISNVSIL
                     KSENYIEINGIRCYQWRVKNLATQTETTYWVSETKYDFAPKLFSALANAGSAIEELVS
                     LPQPEGILPLMVVDRTRFRKLRSNIRVLSITPMQISQTLFTIPVGFNELISS"
     gene            complement(1527..2450)
                     /locus_tag="PK049_10085"
     CDS             complement(1527..2450)
                     /locus_tag="PK049_10085"
                     /inference="COORDINATES: protein motif:HMM:NF036772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HOK61948.1"
                     /translation="MENITPPYLQPGDTIGIVAPARSITRDELQPAIRFLTSQGFLVK
                     TAPHLYSKHYQFAGTDGERAQDLMEMIEDQSVKAILCARGGYGSVRILDLLNLRKLQH
                     NPKWIIGYSDITFLHSLITGWFGIETLHATMPINFPDSVQGNQSTMALISALKGETLY
                     YELKHHPLNLPGHATGVLIGGNLSILSSLSGTTSDMSYSGRILFIEDVDEYLYHIDRM
                     IMQLKRSGKLKSIAGLIVGHFSDMKDNSEPFGSDAYEIIYSAVKEYRIPVCFGFPAGH
                     AEPNLPLIIGRTVELKVSDEGAKVGFYRSLT"
     gene            2693..>4710
                     /gene="metG"
                     /locus_tag="PK049_10090"
     CDS             2693..>4710
                     /gene="metG"
                     /locus_tag="PK049_10090"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOK61949.1"
                     /translation="MNSFKRYLITSALPYANGPVHIGHLAGVYIPSDIFTRYLRAKGV
                     EVISVCGSDEHGVPITIKARQENTTPQAVVDKYNAIIKDSFERFGIAFDIYSRTTSKV
                     HYDTASEFFRKLYDKGVFIEKSTEQYYDEEVGQFLADRYIMGTCPHCGNDRAYGDQCE
                     KCGTSLSPTDLKDPRSMLSGSKPVMKETKHWFLPLDRFQPDLEKWILEEHKEWKPNVY
                     GQCKSWLDQGLQPRAVSRDLDWGVPVPVEGATGKVLYVWFDAPIGYISATKELTPNWE
                     KYWKDPETRLIHFIGKDNIVFHCIIFPAMLKAEGSYILPDNVPANEFLNLEGDKISTS
                     RNWAVWLHEYLDEFPGKQDVLRYVLTANMPETKDNDFTWKDFQTRNNSELVAILGNFV
                     NRAMVLTQKYFDSKVPAIAELDNYDRETLAEIPKLKQSIEQNLDEFRFREALKEAMNL
                     ARLGNKYLADTEPWKVYKTNPQRVATILNISMQITANLGVLLEPFLPFTAEKIRAMVN
                     LNGKDWNLAGGDNLLLPGHQLNEPSLLFDKIEDDVIERQLEKLRKAKEANERANIKVT
                     PLKDSVTYDEFSKMDIRIAKVLEAERVPKTQKLLKLKIDTGVDTRTIVSGIAEFFTPE
                     QMVGKQVVVLANLEPRTIRGIESHGMILMAEDVDGRLQLVIPADSVSNG"
     assembly_gap    4711..4810
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4929..6230
                     /locus_tag="PK049_10095"
     CDS             4929..6230
                     /locus_tag="PK049_10095"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HOK61950.1"
                     /translation="MKRNILLLSIFVLGTMVAMADYNPANNGSASKMKTGNRNASTTT
                     DAPFYNPAGTVWGNDGFALEFSTIPFYSTKSIFDDGNFIGNILEYKSKTSSLLYPALN
                     LTYKKDRFALFMFAGITNGGGAGNYNDGLPTFERLGYFNVITGIMGGLLPGAVTDYGV
                     KTSFKGSAYGVGGWLGAAYRVNDWLSVGGAFQYSRQMNHQEGWLDITYTPAPSAVPIP
                     RTEIDVDFTGQSFGFIGSVDLKPMPNLLIANTFRYFTEMELETKVNDNKSGGLFTDGD
                     KVKSTYVPTYNLGVAYNANEKLTLHYNMNVSLYSMLSLDEDVDGVNVAEYYHNGIDFG
                     VAAEYQFTEKFNWGVGCTYAPYKMKRQYQSEMEFENNTIWLNTGASLKLKENLGIDLG
                     FQVGLATQERKVESTEWVAPFNQKYENGPSYSMGIGFWYKF"
     gene            complement(6345..6593)
                     /locus_tag="PK049_10100"
     CDS             complement(6345..6593)
                     /locus_tag="PK049_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61951.1"
                     /translation="MGTPWVELPRDTIRHAFGVTHVVSLTKPTATFHPRPIAGSDFYA
                     IAKIIPLGLRNFTNRRNENTKRCFDKVYPEISGLSMTA"
     gene            7033..8130
                     /locus_tag="PK049_10105"
     CDS             7033..8130
                     /locus_tag="PK049_10105"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOK61952.1"
                     /translation="MELNAIVQKILQTNDYVSLPGLGSIVRKYESAQLANDGKTLLPP
                     KEYFVFDPNRTFNDEALENYLEASYGLSKSEASRRVEEFCQTLLNSLKQNKTVSIPGI
                     GSLKTGVDGGIELIADDALATTFLPPVDIVVNHQGTEKVEANQDLGSSMPTYRKEDKS
                     TPTVNVEATPSYIPGTPKKSQRSFAAGLFAAVMVIGVVAAFIFISELRFWEGNKSESV
                     SQVAVVVPDESIKPSNYEQPVSEVKSDSASVQNMATDSSINAFPRSEVKQHTSVIVPI
                     DKKSALLFQDKPIEESKTFYIVIGSFASRDNAQKLIDEVSRLGYKPFILPGNNVYRVV
                     IYQFTNRDRALRELERVRGLHLTSQAWLLTL"
     gene            8161..8517
                     /gene="folB"
                     /locus_tag="PK049_10110"
     CDS             8161..8517
                     /gene="folB"
                     /locus_tag="PK049_10110"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOK61953.1"
                     /translation="MAIIEIENMEFYAYHGCYKEEQVVGNHFQVSILIEVETQAAQLT
                     DNLNDTVNYQVVYNLIKEEMQLKSKLLEHVAERIVSAIHNNFKNVERVWVKVSKLNPP
                     LGGKVEKVSVSLERKF"
     gene            8572..8642
                     /locus_tag="PK049_10115"
     tRNA            8572..8642
                     /locus_tag="PK049_10115"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8604..8606,aa:Cys,seq:gca)
BASE COUNT         2526 a         1756 c         1904 g         2552 t
ORIGIN      
        1 ctcagcatga caaagggctt tttccctaac gctcaacatg atatatcctt aactaacgta
       61 tataggaaat ttattcttga atcacttctt ctttctacta cctaatttac gtttcccctt
      121 gtttaagcaa acacaattat aggaatggtc aatccaatcc aaaatcaagg cgtcgggtat
      181 tgatccatct attgctatag tgttccagtg gcgtttattc atgtgatagc cgggttgaac
      241 agccatgtat ttttcacgga gctctatggc gagttcagga tcgcatttaa ggttaagctg
      301 tgtgtcccca ctaaggttaa ccatggcaaa gattttccca ttcaccttga aaaccagggt
      361 gtcgtcgtcg aacggaaaac tttcagttac atctggtttt gatatgcaat gctctctaat
      421 cagttcaata ttcataggtc tgtaattgct gaagattata gtgttgggaa aaatgcatct
      481 tcaatatgct caatctcagg tggtaagcca tggttgatta caattgaaac aatatcgaat
      541 cgtacatcgc aatcaaggtt atgtctttga acatactcgt cggcagcttc aacaatcatt
      601 ctttgctttt tccgtttaac cgattccttg ggctcttccc agtagtgggt acttcgggtt
      661 ttaacctcca ctataacaag ggtgtcgttt agttgtgtca ccaaatcgag ttccttgtgg
      721 ccaaagtgcc agttcctgtg taaaatttta tatccctttg cagccaagta acttgcagca
      781 atttcttcgc ccttactgcc taaatccttg ttgccaccaa ttttttctac cattctatga
      841 aacaattttt aacaaaaatc gatataaatt tttgttgatt aactcttttt ttgggcttgc
      901 ggtttatttg ctacatagca ttcttcactt tactgctatc cacaaacgcc tactcaaagg
      961 taggcggttt tgagggtaaa gtcaatattg ttcgtgaggg tgtatacgat accatacagg
     1021 ttgaggttta cattaagggc aacctaatac gtgttaacga gtttagtaaa ggtggtaagt
     1081 tgatacgatc ctactttgcc aatgtggaaa gtggatcaac ctatctggta tcattcagca
     1141 ataggaagta ctccaaagtt aaggtagata attctcccgc aatttcaaat gttagtattc
     1201 taaagtcaga gaactatatt gaaattaatg gtatcaggtg ttaccagtgg agggtaaaaa
     1261 acttagccac tcaaactgaa acaacatatt gggtatcgga aacaaagtac gactttgctc
     1321 ctaaactttt cagtgcattg gccaatgctg gatcagctat tgaagaattg gtaagtttac
     1381 cacaacccga agggatattg ccattgatgg tagttgatcg tacccggttc cggaagctgc
     1441 gaagcaacat aagggtttta agtattacgc ccatgcagat ttcgcaaacc ctgtttacta
     1501 ttccagttgg gtttaatgag ttaatatcaa gttaaactcc tgtaaaatcc tacttttgcc
     1561 ccctcatcgc taacctttaa ttcaacggtt ctaccaatta ttagagggag gttaggctct
     1621 gcgtgtccag caggaaatcc aaaacaaaca ggtattcggt attccttaac agcgctgtaa
     1681 ataatttcgt aagcatcgga accaaaaggt tcgctattat ccttcatgtc gctgaagtgt
     1741 cctacaataa gcccggcaat tgatttaagc ttgcctgaac gctttagctg cataatcatg
     1801 cggtcaatat ggtagaggta ttcatcaaca tcttcaatga atagaattct accgctatag
     1861 gacatgtcgc ttgttgtgcc actgagcgat gaaaggatgg aaaggtttcc gccaatgaga
     1921 accccagtgg catgtccagg aaggtttaag ggatggtgtt ttagttcgta gtataaggtc
     1981 tcacctttta aagcgctaat cagcgccatg gtcgattggt ttccttgaac agaatcgggg
     2041 aaattgatgg gcatggtggc atggagcgtt tcaatgccaa accaaccggt aattaagctg
     2101 tgcaagaagg tgatgtcgct atagccaata atccattttg ggttatgttg aagcttccgc
     2161 aagtttaata aatccaatat tcttaccgag ccgtatcctc cgcgagcaca aagaatagct
     2221 ttcacggatt gatcttcaat catctccatt aaatcctgag ctctctcgcc atcggtgccg
     2281 gcaaactggt agtgtttaga gtataggtgt ggtgctgttt taactaaaaa accctgtgat
     2341 gttaaaaacc taatggccgg ctgtagctca tcgcgggtta ttgatcgagc tggagcaaca
     2401 atccctattg tatcgcctgg ttgtaggtat gggggtgtta tgttttccat gcacctgttg
     2461 taaaaaaatg gactttaaag ttagattatt ttttgaaata aatctcattt ttgtcattaa
     2521 ggttttactt attcaccttg aaatactttt cggttcttta aagaaaatca attagatttg
     2581 tggcactaaa atagctgggt atggatacat atgcattagt tgcctacaaa gtcacaccta
     2641 cagctttttt agtaaacacc attgaaatac taagttattt aatacacgac agatgaactc
     2701 atttaagcgt tacctgatta catcggcttt gccctatgca aatggacctg ttcatatagg
     2761 tcaccttgcc ggcgtttaca taccatccga tatttttacc cgctacctaa gagctaaagg
     2821 agttgaggta atctcggtgt gcggatccga tgaacatggg gttcctatta ctattaaggc
     2881 acgtcaggaa aacaccacac cccaagctgt tgttgataag tataacgcaa tcatcaagga
     2941 ttcctttgag aggtttggca ttgccttcga catttactcc cgaacaacct caaaggtgca
     3001 ctatgacact gccagtgaat ttttccggaa gctatacgat aagggtgtat tcattgagaa
     3061 atcaactgaa cagtactacg atgaggaggt aggtcaattc ctggccgata ggtatatcat
     3121 gggaacttgc ccacattgcg gaaacgaccg cgcctacggc gaccagtgcg aaaagtgtgg
     3181 cacaagcctt agcccaaccg acctaaagga tccccgatcc atgctcagcg ggtcaaagcc
     3241 tgtgatgaag gaaaccaagc actggttctt accactagac cgtttccaac ccgatttgga
     3301 aaaatggatt ctggaagagc ataaggaatg gaaaccaaat gtttacggac agtgcaagag
     3361 ttggctcgat caaggcttac agccacgtgc cgttagccgc gatttagatt ggggtgttcc
     3421 tgttcccgtt gaaggggcta ccggaaaggt gctatatgta tggttcgatg ctccaattgg
     3481 atatatttct gcaaccaagg agttaacgcc aaactgggaa aagtactgga aggaccctga
     3541 aaccaggctg atccatttta tcggaaagga taatattgtt ttccattgca tcattttccc
     3601 tgctatgctt aaggcagagg gtagctatat actacccgat aatgttcctg ccaatgagtt
     3661 ccttaacctt gagggcgata aaatatctac ttcgcgtaac tgggctgtat ggctccatga
     3721 gtacctcgat gagtttcccg gtaaacagga tgtgctcagg tacgttctta cggccaacat
     3781 gcccgaaacc aaggacaacg atttcacctg gaaggatttt caaacccgta ataacagtga
     3841 attggttgca attctgggta actttgtaaa ccgtgcaatg gttcttacac agaagtattt
     3901 tgattcaaag gtgcctgcaa ttgctgaact tgacaattac gaccgcgaaa cccttgcaga
     3961 gatacccaaa cttaaacagt ccatagagca aaaccttgat gaattccgct tccgcgaagc
     4021 attaaaggag gcaatgaacc ttgcccgtct tggtaataag tatcttgccg acactgaacc
     4081 ctggaaggtt tacaaaacaa acccacaaag ggttgcaacc atactgaaca tttccatgca
     4141 gattactgct aaccttggag tactattaga gcctttcctt ccatttaccg ctgaaaagat
     4201 tagagcaatg gtaaacctaa atggaaagga ctggaatctg gcaggtggcg ataatctttt
     4261 attgcccggt caccagctga acgagccatc gcttctgttc gataagattg aagacgacgt
     4321 tattgagcgg cagctcgaaa agttacggaa agctaaagag gctaacgaga gggcaaatat
     4381 caaggtaacc cccttgaaag attcagttac ctacgatgag ttttccaaaa tggacattcg
     4441 cattgctaag gttcttgagg ctgagagggt accaaaaaca caaaagttgt taaagctgaa
     4501 aattgatacc ggagtcgata cccgtactat tgtttcgggc atagctgagt tctttacgcc
     4561 cgagcaaatg gttggaaaac aggtagtagt tctggcaaac cttgagccaa gaaccatcag
     4621 aggaattgag tcgcatggaa tgattcttat ggccgaggat gtagatggaa ggttacagct
     4681 ggttataccg gccgattcgg tatcaaatgg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn caccatttaa aaacaacttt tgttaacgct cattaacaat tgggttaggg
     4861 gtgaaatttc tacctgcccg acatgttgag ctgaattttt tgaaaatcta accttaaacc
     4921 aaactgatat gaaaagaaac attttattgc ttagcatttt tgtgcttgga accatggtgg
     4981 caatggccga ttacaatcct gccaataatg gtagtgcttc caaaatgaag accggaaacc
     5041 gaaatgcatc aaccacaacc gatgccccct tttacaaccc agcaggaacc gtttggggaa
     5101 acgacgggtt tgccctcgag ttctctacaa ttcctttcta ctccaccaag agcattttcg
     5161 atgatggtaa ttttatcggc aatattttag agtacaagag taaaaccagt tcgcttcttt
     5221 atccagcact aaatcttacc tataaaaagg ataggtttgc gctatttatg tttgcaggaa
     5281 tcaccaatgg tggtggcgcc ggcaattata acgatggatt accaacattt gagcgtttgg
     5341 gttatttcaa tgttattact ggtataatgg gtggattgct gcctggcgca gtaaccgatt
     5401 atggtgttaa aactagtttt aaaggttctg cttatggtgt agggggttgg ctaggtgcag
     5461 cctatcgtgt taacgattgg ttatcggttg gtggcgcttt tcagtattcc cgccagatga
     5521 accatcagga gggttggttg gatatcacct atacccctgc accatctgct gtacctattc
     5581 caaggactga aatagatgta gattttacag gtcaaagttt tggttttatt ggaagtgttg
     5641 acctaaaacc tatgccaaat ctactaatag caaatacttt ccgctacttc actgaaatgg
     5701 aacttgaaac caaggttaat gataacaaaa gtggtggatt atttaccgac ggcgataagg
     5761 tgaaatcaac ctatgtccca acatataacc ttggcgtggc ctataatgct aacgaaaagt
     5821 taaccttgca ctataacatg aatgtttcac tatactccat gctctcactc gatgaggatg
     5881 ttgatggagt taacgttgcc gaatactacc ataatggtat cgattttggg gtagcggctg
     5941 agtaccagtt taccgaaaag ttcaactggg gtgttggctg tacttatgct ccctataaga
     6001 tgaaaaggca atatcaatcg gaaatggaat ttgagaataa caccatttgg ctcaacactg
     6061 gcgctagctt aaagctaaag gaaaacttag gtatcgactt agggttccag gtaggtttgg
     6121 ctacacaaga gcgtaaagta gaatcaaccg aatgggttgc tccttttaat cagaaatatg
     6181 aaaacggacc atcatactct atgggaattg gtttctggta caagttttag gaagggtaga
     6241 tagtctatgt aatagaggtt gtcctaatgg gcaacctcta tttctatctt aatttcagtg
     6301 ttgtttagtt aaggttatta gtcatgttac gcgcaagcga aacattacgc tgtcatgctg
     6361 agtcccgata tttcgggata gactttgtcg aagcatctct ttgtattttc attccttctg
     6421 ttagtgaaat tcctcaaccc caaaggaatg attttagcaa tggcataaaa gtcagacccg
     6481 gcaatgggtc gggggtggaa cgtggcggtg ggttttgtaa gcgataccac atgagtcacg
     6541 ccgaaggcgt ggcgaattgt atcgcgtggc agttccaccc acggggtacc catcgggtta
     6601 ggcttttcag ccttgatttt ctttggttct ttcttgtatc aagacaagaa agaacgttta
     6661 aatagctctt tgtaatgctt ttcttcagcg atgcaacgtt ttccccttgc tctttggaaa
     6721 atgtattgcg tgcatcgcta tacgtgtggg agtagccatg gtgttacaaa tgtttagccc
     6781 ctacggggct ggaaacgttg ttcgtgaaac tattgtcatg ttgagctaag cgaaacatct
     6841 catgggatcg attagagatc cttcgcttca ctcaggatga caaaaggtat gtatattttg
     6901 ttaactaaac gacattgatc tttatttaaa aaaaagttga atgtattttt ttgaaaacca
     6961 taacccaacc attttgattt aacccaaata attttataca tttgttagtt aaaccccaaa
     7021 caccctacag cattggaatt gaatgctata gtgcaaaaaa ttttgcaaac caacgattat
     7081 gttagccttc cagggcttgg cagcattgtt cggaagtacg aatccgctca gctagccaac
     7141 gatggcaaaa cgcttctgcc tcctaaggag tactttgttt ttgaccctaa ccgaacattt
     7201 aacgatgagg cacttgagaa ttaccttgaa gcaagttatg gcctttcaaa aagtgaggca
     7261 agcagaagag ttgaagagtt ttgtcaaact cttttgaata gcttaaaaca gaataaaaca
     7321 gtttctattc ccggaatagg ttcgctcaaa actggggttg atggaggaat tgaactgatt
     7381 gccgatgatg ctttagcaac aacattccta ccaccagttg atattgttgt taaccatcaa
     7441 ggaactgaaa aggttgaagc taatcaggat ttaggttcat ccatgccaac ttatcgcaag
     7501 gaagataaat ccactcctac agttaatgtt gaagcaaccc catcatatat tcctggaact
     7561 cccaaaaagt cccagcggtc gtttgcagca ggtttatttg ctgctgttat ggttataggc
     7621 gttgttgcag catttatctt tatttctgag ttaagattct gggaagggaa caagtccgaa
     7681 agcgtttcgc aagtggcagt ggttgttcct gatgaaagta tcaagcctag taattatgaa
     7741 caaccagtaa gtgaggttaa atccgattcg gcttcagttc aaaatatggc aaccgatagt
     7801 tccattaatg cttttccaag gagcgaggta aaacaacaca caagtgtaat agttccaatt
     7861 gataaaaaaa gtgccctttt atttcaggat aaaccaatcg aggagagtaa gactttctat
     7921 attgtaattg gcagttttgc tagccgcgat aacgcccaaa agttaattga tgaggtgtcg
     7981 cgtttgggct ataagccatt cattttaccg ggcaacaatg tttacagagt ggtaatttac
     8041 caatttacta atcgcgatag agccttgagg gaattggagc gagtgcgtgg tttgcactta
     8101 acctcacaag cctggctttt aaccctttag ctatactcca cacacatttt atcttaaccg
     8161 atggcaatta ttgagattga gaacatggag ttttacgctt accatggatg ctacaaagag
     8221 gaacaagttg tagggaatca ttttcaggta agcatcttaa ttgaggtcga aacacaagcg
     8281 gctcaactta ccgataattt aaatgataca gtgaattatc aggttgttta caatctgatt
     8341 aaagaggaaa tgcagctaaa atctaagctg cttgagcatg ttgcggagcg tattgtttca
     8401 gctatacaca ataacttcaa aaacgtggaa agggtatggg tgaaagtttc aaagttaaac
     8461 ccaccactcg gaggaaaagt tgaaaaggtg agtgtttcgc ttgaacgcaa gttttgaaaa
     8521 cccacaaaaa tttgcaggat taattttttt tgtatctttg cagtgccaaa gggtaccgtg
     8581 gccgagaggt taggcactgg tctgcaaaac cacgtacagc ggttcgaatc cgctcggtac
     8641 ctcaaaaaag cccccaaatg gggctttttt atttctttcg attgtgtaaa ccgcactcct
     8701 tggtctcggg gttttcccac caccaacggc cagctctaac atcctcaccg ggttcaatgg
     8761 ctctggtaca aggttggcag ccaatgcttg gaaaaccttt gtcatgcaag gggttgtagg
     8821 gcaccccatt ggctttaa
//
LOCUS       DAOSAL010000087         8800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_5208_length_8800_cov_49.585706, whole genome shotgun sequence.
ACCESSION   DAOSAL010000087 DAOSAL010000000
VERSION     DAOSAL010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 8800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8800
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5208_length_8800_cov_49.585706"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="PK049_10120"
     CDS             complement(<1..557)
                     /locus_tag="PK049_10120"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HOK61954.1"
                     /translation="MKNRIYLLILFFGIFYSCSLISDYTLGDMAERRVNLPATFEKID
                     IKGSFSITLLPDTVGFAVVKCHEKVMHDVEVKIQNNTLFLRENVKSRWLKDYPIIDIE
                     LHLTEIPMIEIHQPCKFTIPYTFKSHAFYLVDWGNYVDCDVNIEVDFLKIDVSGKSFG
                     TYRATGQAEHATLYARGAALFDLSQT"
     gene            complement(547..1665)
                     /locus_tag="PK049_10125"
     CDS             complement(547..1665)
                     /locus_tag="PK049_10125"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HOK61955.1"
                     /translation="MLLKRKLFFTLTLALFVLLHSGKAQEGRYSIDIKERPGFIIPHH
                     SHIAYFLKENVNAIQLNFGITTDGSKFWHKEFNYPIVGLGLYYSGLGNDTLYGKLTGL
                     YTFINRPFLDNTKRFNISNTLSVGLSYASKWHNPISNRSNMVLSTPLNVFFQYEIGCN
                     YRISTNHNVSASIGLTHASNGSVKEPNLGFNIFTTALGYRYSFGNPNPVRSELPDDIT
                     PFSSWNFGLFASIKSIDAFSGKQYGIIGLSAEKLYRFAPLSMAGLELTAYKDNSIPDL
                     VMDKSEDAVTLNKNDNLALTINATYLMGFNRIGIVFQPGIYLRNQYYGFGAFSNKVGL
                     RINIYDGLILGVAIKAHWFAQADFVEFGIKNSFNRHEK"
     gene            complement(1665..2276)
                     /locus_tag="PK049_10130"
     CDS             complement(1665..2276)
                     /locus_tag="PK049_10130"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOK61956.1"
                     /translation="MNRVLLLGFVLLCLGSTQMQGQSFRGGLLAGVAATQVDGDTYGG
                     YDKAGPIAGFWVERSKLSGWFYRLSFRFIQKGSYAKSRSTDIPDFYRMRLNYFDLPVL
                     AGYRFVNGFNAVLGLSGGYLSKATEENSLGPYPQEEVSTFKKFEIAGIAGMEYNYSSK
                     WRFGLNLSYSILPVRPYKDNISYRMNNGQYNRVIEFVSIYRIQ"
     gene            complement(2269..2958)
                     /locus_tag="PK049_10135"
     CDS             complement(2269..2958)
                     /locus_tag="PK049_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HOK61957.1"
                     /translation="MTTRKRLVVLTGAGISAESGIRTFRDMGGLWEEYDVMEVASIDG
                     WKKNPQLVLEFYNQRRRQLLTVEPNAAHKVLAEVQNLFNVFVITQNVDNLHERAGSKN
                     ILHLHGELTKARSTGNPSRIIDIGYNDIKWGDTVDGYQLRPHIVWFGEEVPMMNEAVK
                     LVQTAEIFAVIGTSLNVYPAAGLLDHAPKGCPIYLVDPNDINIWRRDVTVIKEKASVG
                     VPKLIEMIKNE"
     gene            complement(2955..4160)
                     /locus_tag="PK049_10140"
     CDS             complement(2955..4160)
                     /locus_tag="PK049_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61958.1"
                     /translation="MKRHALLLALILLVAQAFACTTAIISGKATPDGRPLLWKHRDAD
                     DFNNKVVFEVGPRFKYLALINSSDPERHAWAGTNNAGFAIMNSASYNIKPKTDSSKVE
                     DLEGYIMKLALGSCATITDFAQMLDTLPKPWGVSANFGVIDAKGGAGYFEVNNFSYRF
                     YDANSTTDAPEGYLIRSNFSVSGEPNKGAGYIRFHTASNLFKEAKAANGLTHEFLLTK
                     ASVSLRQDITGIDLSKEVSKAEKIYPFRDFIVRYHSSSTVVVQGVLPKEPTDFATMWV
                     KLGFQPASVAIPLWVGAGGILPDIITAPDGLNAKLCDFSLKLKKDCFPLSSWTEGENY
                     VLLSKLINKEQTGIIQLTNIFDKEIIARTKTLYDKWTKFEFNPIEAYNFYLTLNRDVE
                     KFYKNHFDL"
     gene            4238..4759
                     /locus_tag="PK049_10145"
     CDS             4238..4759
                     /locus_tag="PK049_10145"
                     /inference="COORDINATES: protein motif:HMM:NF022766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3124 domain-containing protein"
                     /protein_id="HOK61959.1"
                     /translation="MRYSTLIIALALGVLFGCKHHEKQTVKLAKFDPVHIKVKNPVKK
                     VRAQTLYLPVYSSIPFAEGNSFYGLSAFMAVHNTDFNNPIYIISVYYLDTNGNLIKDF
                     LQSRVDTVMPMQSVNYFVPESDRSGVGANFILEWMADSAVNEPLVETIMVSLTSGQGV
                     SFLSEGKILNELY"
     gene            4764..6245
                     /locus_tag="PK049_10150"
     CDS             4764..6245
                     /locus_tag="PK049_10150"
                     /inference="COORDINATES: protein motif:HMM:NF003716.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HOK61960.1"
                     /translation="MNFTTENFLLIGSLLLFISLIAGKTSFRLGVPTLIFFLFVGMLA
                     GTSFSGLVVFNDAITAQFIGVVALNFILFAGGFETNWFSIKPILWHGVTLSTLGVVLT
                     ALFSGLITWLVTDFSITESFLIGSIISSTDAAAVFSILRSKNLALKGNLRPTLELESG
                     SNDPMAYLLTIIFLGLATSPSLSFWEVVKLFLSQIIIGVSMGVGFGYASKYIINNIKL
                     EYEGLYPVLAITLMLLTFSITDFIGGNGFLAVYLSAILLGNSHIMHKKIILKMFDGLA
                     WLMQIILFITLGLLVNAGEIIPHIGNGLLIAFGLMFIARPLSVFISLSPYRMKNRERW
                     YIAWVGLRGAVPIVLATYPLIAGMAKAQEIFNIVFFITITSVLIQGTSLIRVAKWLRV
                     LLPQRVKQITPVDVMLRENISTEMVEVDIPDDSCVVGKRIVDLNFPEKSLIVLIQRND
                     RYISPTGSTVIEPNDKLIIVAEDKTSLKESFSCLSLDANTYQR"
     gene            complement(6226..7251)
                     /locus_tag="PK049_10155"
     CDS             complement(6226..7251)
                     /locus_tag="PK049_10155"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61961.1"
                     /translation="MKSKLTEWADLVEPVRSVPESERWGYRTKTTLSARFNGNIWEFG
                     MWRRDELVPIPNCPVHAPELNRVLALLADSFPREHSFALAYVVISGAQVVLVIKSKQE
                     PTLTWLSEDVAQGLASMGIEGIWYHMNPSTGRRIFEKSGWMLIWGKTRSTDYHGSLYG
                     PAAFQQLIPELYNESLEEAERFLNPNGNSALIDLYCGTGNSMRRWINRGANVIGAELG
                     GEAVECARLNAPQAMVLRGACRQRVPQIAEWTDKNRYQGKEILLYVNPPRTGLEPEVL
                     EWISKQGLPNRIAYLSCSPGTLSKNLNWLCTNGYRLVRCVPFDFFPQTHHVETLALLE
                     RVSADTY"
     gene            7889..8290
                     /locus_tag="PK049_10160"
     CDS             7889..8290
                     /locus_tag="PK049_10160"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HOK61962.1"
                     /translation="MSVLLLLASKADTLVYQDSPEAIVKKSAEAIEKGDVENLTRNFF
                     KTVEIDLLGDENFYSQAQAVLLLRSFFEKNIPVKFTVNHQGAKDLTAFAIGTLQCKNG
                     LFRVSIFMKTEQGKTYIHQFRIEPETAINPK"
     gene            8295..>8800
                     /locus_tag="PK049_10165"
     CDS             8295..>8800
                     /locus_tag="PK049_10165"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225316.1"
                     /note="catalyzes the formation of
                     pyridine-2,3-dicarboxylate and 5-phospho-alpha-D-ribose
                     1-diphosphate from nictinate D-ribonucleotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide diphosphorylase
                     (carboxylating)"
                     /protein_id="HOK61963.1"
                     /translation="MTPYDQLIDRLIDLAIAEDLGDGDHSSLSCIPYDAVGAVQLMMK
                     QEGVIAGLRVAERIYKRIDGSISINFLKSDGELLQSGDIAFRAYGRVHALLQAERIVL
                     NVLQRMSGVATQTREYVKLCEGTKAKVLDTRKTTPGMRVLDKMAVAIGGGLNHRMGLF
                     DMIMLKDN"
BASE COUNT         2384 a         1840 c         2008 g         2568 t
ORIGIN      
        1 gtttgcgaaa ggtcgaaaag agcagcacca cgagcataca gtgtggcatg ctcggcctgg
       61 cctgttgccc ggtatgttcc aaagctcttg cctgaaacgt cgatcttaag aaaatcaact
      121 tcaatgttta catcgcaatc aacgtagttg ccccagtcca ccaagtaaaa tgcatggctt
      181 ttaaaggtgt aggggatagt aaatttacag ggttggtgga tttcaatcat ggggatttcg
      241 gttaggtgta actcaatgtc gataatgggg tagtccttta gccatcggct ttttacattt
      301 tcccttagga atagcgtgtt gttctgaatt ttaacctcaa catcgtgcat aaccttttcg
      361 tggcatttaa ccaccgcaaa accaacggtg tcggggagta gggtgatgct aaacgaaccc
      421 ttaatatcaa ttttttcaaa ggtggcgggt aggtttacgc ggcgctcggc catatcgccc
      481 agtgtataat cgcttatcag gctacaggaa tagaaaatgc caaaaaaaag tatcaataag
      541 tatattctat ttttcatgcc tattgaaaga gtttttaatt ccaaattcaa caaaatcggc
      601 ttgggcaaac caatgagctt taatggcaac tcctaggatt aagccgtcat agatattgat
      661 gcgcaatccc accttgttgg aaaatgcgcc aaagccataa tattggtttc gcaggtaaat
      721 accgggctga aaaacaatac caatgcgatt gaagcccatg aggtatgttg cattgattgt
      781 caaggccagg ttgtcgtttt tgttaagtgt aaccgcatct tctgatttgt ccattactaa
      841 gtcgggtatc gagttatcct tgtaggctgt tagttcaagc cctgccatgg ataggggtgc
      901 aaagcggtat agtttctcgg ccgataggcc gataatgcca tactgttttc cgctgaaggc
      961 atctatcgat tttatgctgg cgaacagtcc aaaattccat gatgagaatg gggtgatatc
     1021 atcaggcaat tcacttctta caggatttgg atttccaaat gagtaacggt aacccagtgc
     1081 ggtggtgaaa atgttaaatc ccaggtttgg ttcctttacg cttccgttcg atgcatgggt
     1141 aagtccaata cttgccgaga cattatggtt tgtgcttatc ctgtaattgc atccaatctc
     1201 gtactggaag aaaacgttca ggggtgtact tagtaccatg ttgctgcggt tgcttatggg
     1261 gttatgccat tttgaggcat agctcaaacc taccgagagc gtgttactaa tattgaaccg
     1321 tttggtgttg tccaaaaatg gccgatttat gaaggtgtat aaacccgtaa gtttaccgta
     1381 aagagtatcg ttccccaagc cagaatagta tagccccaag cctacaatgg ggtagttgaa
     1441 ctccttatgc cagaacttgc taccatcggt ggttatgcca aagttaagtt gaatggcgtt
     1501 cacattttcc ttgagaaagt aggctatgtg gctatggtgc gggattataa atccgggccg
     1561 ctcctttata tcgatactgt acctaccttc ctgtgctttg ccgctatgaa gtaacacaaa
     1621 aagagcaagc gtaagagtaa aaaacaattt gcgtttgagt aacatcactg tatcctgtaa
     1681 attgaaacga actctataac tcgattatac tgcccattgt tcattcggta cgatatatta
     1741 tccttgtaag gcctaacagg caggatggaa taggaaaggt taaggccaaa cctccatttg
     1801 gagctgtagt tatactccat gccggcaata ccggcaattt caaatttctt aaaggttgat
     1861 acttcctcct gagggtatgg cccaagcgaa ttctcttcgg tagctttgct caggtagcca
     1921 cccgaaagcc ccaaaacggc attgaagccg tttacaaaac gatatcctgc cagtacaggt
     1981 aggtcgaagt agttgagacg catgcggtaa aaatcgggaa tatcggtaga gcgactttta
     2041 gcataactac ctttttggat gaaccggaaa cttaagcggt aaaaccatcc ggaaagctta
     2101 ctgcgttcta cccaaaagcc cgcaatgggt cctgccttgt cgtaaccgcc gtaggtatcg
     2161 ccatcaactt gcgtggcggc taccccggct agtaggcctc ccctaaagct ttggccttgc
     2221 atttgagtgc tccccaaaca cagtaaaaca aaccccaaga gtagtaccct attcattttt
     2281 aatcatctca ataagttttg gaacccccac cgatgctttc tccttaatca ccgtaacatc
     2341 gcgccgccag atatttatat cgtttgggtc aactaggtat atgggacaac ccttaggggc
     2401 atgatcgagc agtccagctg cagggtaaac attcagcgag gtgccaataa cagcaaaaat
     2461 ctcggctgtt tgaaccagtt taacagcctc gttcatcatg ggaacttctt cgccaaacca
     2521 aacaatgtgc gggcgtagct ggtatccatc aacagtatcg ccccatttaa tgtcgttgta
     2581 gccaatatcg ataattctac ttgggttgcc ggttgacctt gccttggtta actcaccgtg
     2641 aaggtgaaga atatttttac tgccggcacg ctcatgcagg ttatcgacat tctgggtgat
     2701 tacaaatacg ttgaagaggt tttggacctc ggcaagcacc ttgtgggctg cattgggctc
     2761 aacggttagc agttgtcgcc ttcgttggtt gtagaattcc agtacgagct gtgggttttt
     2821 cttccatcca tcgatgcttg ccacctccat aacatcatac tcttcccaaa gccctcccat
     2881 gtcgcggaaa gtgcgaattc cgctttcggc gctaatgcct gctcccgtga gaactaccag
     2941 tcgtttacgt gtggtcataa atcgaaatgg tttttataga acttttcaac atccctgtta
     3001 agggttaggt agaaattgta tgcttcaatg ggattaaatt caaattttgt ccatttatcg
     3061 taaagcgttt tggtgcgggc aataatttcc ttatcgaaaa tgttggttaa ctgaattatc
     3121 ccggtttgct ccttgttaat gagtttgctt agcagcacat agttttcccc ttcggtccat
     3181 gagctaagcg ggaagcagtc cttttttagt ttaagggaaa aatcgcatag cttggcgttt
     3241 agcccatcgg gggcagtaat aatatcgggt agtatgccac ctgcgccaac ccacagagga
     3301 atggcaaccg atgcaggctg aaagcccaac tttacccaca tggtggcaaa atcggtaggc
     3361 tccttgggca gaacaccctg caccactacg gttgacgatg agtggtatcg gactataaag
     3421 tcgcggaaag ggtaaatctt ttcggccttt gagacctctt ttgataaatc aattccggtg
     3481 atatcctgcc ttaggctaac ggaggcttta gttaagagga attcatgcgt tagcccgtta
     3541 gctgccttag cttccttaaa caggtttgat gccgtatgaa agcgaatgta gcctgctcct
     3601 ttattgggtt cgcccgaaac tgaaaagttt gaacggatca aatacccctc gggagcatcg
     3661 gttgtactgt tggcatcgta aaagcggtag ctaaaattgt taacctcaaa gtaacctgct
     3721 ccacccttgg catcgattac cccaaagttt gccgatacgc cccacggttt tgggagtgta
     3781 tcgagcattt gggcaaaatc ggtaatggtg gcgcagctgc ccaatgccaa tttcatgatg
     3841 tatccctcaa ggtcttcaac ttttgatgaa tcggttttgg gtttgatgtt gtacgaagcc
     3901 gagttcatta tggcaaatcc ggcgttgttg gtgccagccc aggcatgacg ctcagggtca
     3961 cttgagttga tgagcgctaa gtatttaaaa cgtggcccaa cctcaaaaac aaccttgttg
     4021 ttaaagtcat cggcatcgcg gtgcttccat agcagcggtc gtccatcggg tgtagcctta
     4081 ccgctgataa tggctgtggt acacgcaaaa gcttgagcga ccaaaagaat caatgcaagt
     4141 aaaagtgcat gccttttcat ggtaaaattg gcttaaatag aactaatttt ttatattgca
     4201 agatatgaaa taattgtcaa actaaaccat attggttatg agatactcaa cgttaattat
     4261 cgccctagct ttaggtgttc tttttggctg taaacaccat gaaaaacaaa ctgttaaact
     4321 ggctaaattt gacccagttc acattaaggt gaagaacccc gtgaaaaagg ttagggcaca
     4381 aacgctctac ttaccggttt actccagcat accatttgcc gagggaaatt cgttttatgg
     4441 gttaagcgcg tttatggctg ttcataatac cgattttaat aatccaattt acattattag
     4501 cgtttactac ctcgatacca atgggaacct gataaaggac tttttgcaat cgagagttga
     4561 tacagtaatg cccatgcaat cagttaacta ctttgtcccg gaaagcgaca ggagcggagt
     4621 gggcgcaaat ttcattctgg agtggatggc cgattctgct gtaaacgagc ccctggttga
     4681 gaccatcatg gtaagtttaa ccagtgggca gggagtatcg tttttaagcg aaggtaaaat
     4741 tttgaacgaa ttgtattagg ctgatgaatt ttaccaccga aaactttttg cttattggtt
     4801 cgttgctgct atttattagc ttaatagccg gaaaaacatc gttccgcttg ggtgtcccaa
     4861 cgctcatctt ttttttgttt gttggaatgc ttgcaggcac gtctttttca ggactagttg
     4921 tttttaacga tgccattact gctcagttca ttggggttgt tgccttaaac tttattctat
     4981 ttgcaggggg gttcgaaacc aattggttta gcattaagcc gattttatgg catggtgtaa
     5041 ccctttccac cctaggggtg gtgttaacgg ctcttttctc ggggcttata acctggctag
     5101 taactgattt tagtattacg gagagctttt taattggctc aattatatcg tcaaccgatg
     5161 cggcagctgt tttctcaata cttcgctcaa aaaatttagc cctaaagggc aatctgaggc
     5221 caacccttga gcttgagagt ggtagtaatg accctatggc ttatctattg accatcattt
     5281 ttcttggact tgcaacaagc ccatcgctat cattctggga ggttgtaaaa ctctttctaa
     5341 gccagataat aattggagtt agcatgggtg tgggctttgg gtatgcaagt aaatacataa
     5401 ttaacaacat caaactcgaa tatgaagggt tataccctgt attagcaatt accctgatgt
     5461 tgttaacctt ttccataacc gattttattg gagggaatgg gtttttagcc gtttacttat
     5521 cagcaatact tctgggaaat agccacataa tgcataaaaa gatcattctg aaaatgttcg
     5581 atggtttggc ttggcttatg caaataatcc ttttcattac acttggttta ctggtgaatg
     5641 cgggtgaaat tattccgcac ataggaaacg ggttgcttat agcctttggg ttaatgttta
     5701 ttgcacgacc attgagcgtt ttcataagcc tatcccctta taggatgaag aatcgtgaac
     5761 ggtggtacat tgcttgggtg ggattgcgtg gtgctgtgcc tattgtactt gccacatacc
     5821 ccttgattgc cggaatggct aaggctcaag agatattcaa tatagtattt tttatcacca
     5881 taacgtcggt gttaatacag ggtacgtcgc ttattcgagt agcaaagtgg ttaagggttt
     5941 tgttaccgca acgggttaag caaattaccc ctgttgatgt tatgctcagg gaaaatatta
     6001 gcaccgaaat ggtagaagtt gacatacccg acgatagctg cgttgtaggg aaacgaatag
     6061 ttgacctaaa ttttcctgaa aaatcgctta tcgtgctaat tcaacgcaac gacaggtata
     6121 tttcgcctac gggctcaaca gttattgagc ctaacgataa gctcataatt gtggccgaag
     6181 ataaaacctc gttaaaggaa agttttagct gtttgagttt agatgctaat acgtatcagc
     6241 gctaactctc tcaagcaaag caagcgtttc aacatggtgt gtttgtggga agaaatcgaa
     6301 gggcacacat cgtactaatc ggtatccgtt tgtgcagagc caattcaagt tctttgagag
     6361 ggtgcctggg ctacacgata ggtaggcaat tcgattggga agcccttgtt ttgaaatcca
     6421 ttctagaacc tcaggttcaa gccccgtgcg tggtgggttt acgtaaagta atatttcttt
     6481 cccttgatag cgatttttat cagtccattc agcaatttgt ggaactcttt gccggcaagc
     6541 accgcgcaaa accatagctt ggggagcatt caaacgggcg cactccacag cctcgcctcc
     6601 aagctcagcc ccaattacat tagccccccg gttaatccat cgtcgcattg agtttccggt
     6661 tccgcagtat aagtcaataa gagcgctatt cccattaggg tttaaaaacc tttccgcctc
     6721 ctccagcgat tcgttgtaca gttcgggtat caactgctga aaagcagcgg ggccataaag
     6781 tgagccatgg tagtcagttg aacgggtttt cccccaaatt aacatccatc cggatttctc
     6841 aaaaatcctt cggcccgttg aaggattcat atggtaccat atcccttcaa ttcccatgct
     6901 ggcaagtcct tgtgcaacat cttcactaag ccaggttaat gttggttcct gcttcgattt
     6961 gattaccaga acaacctgtg cgcctgaaat tactacgtat gcaagtgcaa aagaatgttc
     7021 ccgcggaaat gaatccgcaa gcagggcgag tactctattc aactccggtg catgtacggg
     7081 gcagttggga attggaacaa gctcgtcgcg ccgccacatt ccaaactccc aaatatttcc
     7141 attaaacctt gcgcttaggg tagttttagt gcgatagccc caacgctcac tctccggtac
     7201 tgagcgcacg ggttcaacca aatcggccca ttcagttagt ttacttttca cgaatgcttg
     7261 tttctgctca aggctatcgg tcatggacca atccctgtgg ctacaccctc tacatttcgg
     7321 attacaaccc tgtggcagca taaacattta tttccgcaaa ggtaatagag ttttgagccg
     7381 aattgccatg aattaatgga ggaatgattc ggaatagaag gaatatgttg gagttagaag
     7441 aagttagaag aagttagagg aagtttaagc ccgttaaacg tgaaccgtga accgttaaac
     7501 gcttgacttt aagcgggtgt gatacggaaa agatggaata tgatggagtt agagggagtt
     7561 agatgaattg tgaaaagtta acagttaaga ccaacaaaaa taaattaggg gtagttcgaa
     7621 atgatacgga ttcatttgga atgattaaat tttgttggtc ggttcgtcaa ttgatttttc
     7681 aattaaccag attgcgtcaa attattttgt ttacactgat tcccaatctt cataaaaata
     7741 cttaattttg aaatgtgcat tttaatttgc aggttgatat tcgctttctg gcttataatt
     7801 tgttgtttag ctattctata ttttaccttt ggggaattaa aagatgaaaa ttaagtacga
     7861 gatgttgcgg aaattaacag tttacttgat gtctgtattg ctgttgcttg catcaaaagc
     7921 cgatacttta gtttatcagg attcgccaga agcaatagtt aagaagagtg ccgaggcaat
     7981 tgaaaagggc gatgtggaaa atcttacccg aaacttcttc aaaacagttg aaatcgactt
     8041 acttggcgat gaaaactttt acagccaagc tcaggcggtt ttactgctac gatcattctt
     8101 tgaaaagaat attccggtta agtttaccgt aaaccatcag ggagcaaagg atttgacagc
     8161 ttttgctatt ggtacacttc agtgtaagaa cgggctattc cgggtatcga tattcatgaa
     8221 aactgagcag ggtaaaacct atattcatca gttccgaatt gagccagaaa cagccattaa
     8281 tccaaagtaa gatcatgacc ccatacgacc aacttattga taggctaatt gacttagcca
     8341 ttgctgagga cttaggcgat ggcgaccact cttccctttc ctgcatacca tacgatgcgg
     8401 tgggcgctgt gcagctcatg atgaagcagg agggtgtaat tgcaggctta cgggttgctg
     8461 agcgcatata caagcgtata gatggaagta tcagcattaa ctttttaaaa tccgatggcg
     8521 aattactcca atctggcgat atcgccttta gggcttacgg cagggttcat gcactattac
     8581 aggctgagag gattgtgctt aatgtgcttc agcgtatgag cggtgttgct acccaaaccc
     8641 gggagtatgt taaactttgc gagggcacaa aagctaaagt gctcgatacc cgcaaaacta
     8701 ctcccggtat gcgtgttctc gataaaatgg ccgttgccat tggtggcggc ttgaatcaca
     8761 gaatgggact ttttgatatg attatgctta aggataacca
//
LOCUS       DAOSAL010000088         8692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_5294_length_8692_cov_49.972097, whole genome shotgun sequence.
ACCESSION   DAOSAL010000088 DAOSAL010000000
VERSION     DAOSAL010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 8692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8692
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5294_length_8692_cov_49.972097"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(349..837)
                     /locus_tag="PK049_10170"
     CDS             complement(349..837)
                     /locus_tag="PK049_10170"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOK61964.1"
                     /translation="MVAIKNPYEGLPEHGCFCCSSKNPIGLKLSFWYDETNQTVETRW
                     LPELNYQGYHGVLHGGIQSTLMDEVASWCIYILEGTAGVTHSMEIVYSKPMLIGKGQV
                     VVRGRIDNREKRLVTVHVELFDGESNLCTTGKIKYFVFPTEIAKSKYGYPGRDAFLDG
                     GE"
     gene            951..1562
                     /locus_tag="PK049_10175"
     CDS             951..1562
                     /locus_tag="PK049_10175"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOK61965.1"
                     /translation="MDTKHGNATRLKLMETARELFIAKGVDRVGVREIASKAGVNLSL
                     MNYYFQSKENLLEQIFEQSIKDNGQKIRQILNADMPLAEKIKVYINTYIDILTEDPLL
                     VPFVLSIIHRNAEQASRLKAVHTLYNTETFSNQLKHEAELGNIRKVDPEQFFVSMISL
                     ILFPFAIKWLIGYRMTLSNEQMAQFLEDRREHVYDLLMSALRP"
     gene            complement(1594..1884)
                     /locus_tag="PK049_10180"
     CDS             complement(1594..1884)
                     /locus_tag="PK049_10180"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HOK61966.1"
                     /translation="MSDHNTKSLGEAIKAFIDAMKWNDNFNQASIRSDWERIVGRDIA
                     MHTTDIQFKEGILTLSLNSAALRQELHMRRKDLLTHINEYFGSEVVKEIRVK"
     gene            complement(1887..2996)
                     /locus_tag="PK049_10185"
     CDS             complement(1887..2996)
                     /locus_tag="PK049_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014298314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HOK61967.1"
                     /translation="MYLKRLTAINFKSINQLDIEFDNKINCFVGNNGQGKTNLLDAIY
                     FLSMCKSSLSSTDLQVIRHGQDFFVLQGFYSRQGVDESIYCGLKQGEGKIFKRNGKAY
                     ERLADHIGFLPVVMVSPADNELVEGSGEERRKYMNGVISQIDHIYLDEVLRYNRLLAQ
                     RNSLLKTYSRTAFSYDLIEAIDIQLAEYASVIHSKRKAFVEQLIGILQEYYNKVSGGQ
                     EKVSLSYKSHLNDTPAETLFRENIEKDRVLQYTSVGIHRDDLTLLIDGYPIRNEGSQG
                     QQKTYLIALKLAQFDFISKVAGIKPLLLLDDIFDKLDTGRVHRLIDMVANDGFGQIFI
                     TDTNKNRLEEVLAKVTSGYRFFRVDNSEFELVESR"
     gene            3118..3810
                     /locus_tag="PK049_10190"
     CDS             3118..3810
                     /locus_tag="PK049_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOK61968.1"
                     /translation="MADKKKQQTTDTVESIDNALTRTEQFIEQNQKSLTIIVAAILAI
                     VAIYFGYKKLYLQPKENEASSQIFQAQYYFEQDSFRLALYGNENDLGFATIADRYGMT
                     RTGNLANYYAGICLRELGEYQKAIDYLKKFDAGDQMVTPIAYGAIGDCYVELNKLKDA
                     AKFYIKAANYEKNDFTTPIFLKKAGMVYEELKQYDKALDLYNIIKNDFTKSIEARDIE
                     KDIARINTLMGK"
     gene            3893..4396
                     /gene="ribH"
                     /locus_tag="PK049_10195"
     CDS             3893..4396
                     /gene="ribH"
                     /locus_tag="PK049_10195"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOK61969.1"
                     /translation="MATYLKNLSSYDPNMVPSAKNMKFGIVVSDWNDSVTHKLLEGAY
                     SALIEHGATDENILVKHVPGAFELTLGGQFMAEYADLDAVICLGCVIQGETRHFDFIC
                     QGVTYGITELNMNYNIPFIFGVLTTDNLEQALDRAGGKHGNKGVEAAITAIKMAALQK
                     EMEELEG"
     gene            4727..7759
                     /locus_tag="PK049_10200"
     CDS             4727..7759
                     /locus_tag="PK049_10200"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOK61970.1"
                     /translation="MKRVFWVFFPLIFAQTISAQQSNPLISKADSASQMRWIDSVFNS
                     LSLRERIAQLFMVAAYSNKDDSHVMEISQLVSKQGIGGLIFFQGGPVRQARQTNYYQS
                     LAKVPLLIGIDAEWGLGMRLDSTISYPRQMALGATNDTLLTYQMAADIAHQFKRMGIH
                     INFAPVIDINSNPLNPVIGSRAFGQDMEMVTRHGMAYTKGLQENGILACAKHFPGHGD
                     TYDDSHLTLPLVPHSANRIDSIELYPFKKLFAAGVASVMVAHLKIPSLEPDTLLPASL
                     SARIVGQKLFGELGFNGLAFTDAMNMKGVADFYNPVDANIRSLMAGNDILLFPSEIEN
                     TIKKVEQLIKSEQIPEELINIKCKKVLMAKYLVGLNKYRPINVDRLYNDLNSPYSQVL
                     RRKIIRNAITVIHNANNILPLRGLDTLRIAYIEIGENKGDAFREQLELYTAVHTFSIN
                     PEAEQAEYDTLLTNLEPYNLIIVGYHTIESKAAKGFGVSQQSSNFLFDLSFRKKVILN
                     LFGNPYSISRFANPLAFAAIIVSYDNSTDSQNLTAQTIFGGIPFSGRLSVTASDLFGI
                     NTGYASNQRIRLGYSIPEELGIGSEQLSEVDSLAMEIIRTGAAPGMQILAAKDGVVFY
                     NKAFGKPTYVSEYGNDIRMLYDIASVTKVAATLPPIMNLYNRGVLSFDSKLGQFIDLR
                     NFPDKSKLLLGDILRHQSGLQAWIPFYMNTLTSLEPNIPLWTETFSQTHPYKYNGKRY
                     ASRFAYPSPKFYRTDSISNFPNNVAYRIYSLASINDSIYRWINQSPISDAGKYRYSDL
                     GFLYLQKVIETVEQKRLDKVVEPLYSKLGMNYTCFNPLLRFDTERIAPTEHDPVFRKQ
                     LIWGHVHDPAAAMLGGVAGHAGLFANANDLAKLMQLYLNDGEYGGERFFAPSTVKSFA
                     NPATTNGNRRAYGFDKPNASGSSPAGTMASPESFGHTGFTGTMVWADPQNGLVFIFLS
                     NRVHPDAENTKLSAMNYRTRIHDVFYRAIQSISKPSGQ"
     gene            8427..8603
                     /locus_tag="PK049_10205"
     CDS             8427..8603
                     /locus_tag="PK049_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61971.1"
                     /translation="MGTPRAELPRDTIRHACGVTHLISLTRSTATFHPRPITGSGFYA
                     TACFFRSLLVEESH"
BASE COUNT         2553 a         1973 c         1746 g         2420 t
ORIGIN      
        1 tgctgccatc aacattgagc atgcggtaag gttatgcttc ataggtaaaa tagttatttt
       61 aaccaaatga cgcatatatg acgcctaatg ctgctcaaag gctactttag aagtgttaat
      121 aattgctaaa agcgtgtgga cgttggcttt gcaaaagagt tacgttgagt atagttgata
      181 gttgacggtt ggtagttgat ggttggtagt tgatggttga tggttgatag ttgacagtat
      241 actgaaatac tattctggaa agatagtaaa cacttaatgc caaaacgttt aacggtttac
      301 ggctaacggt ttatggctaa cggttctcga acctttacta ttttgtcact actcacctcc
      361 gtccaaaaat gcatctctgc cgggatatcc atactttgac ttggcgattt cggttggaaa
      421 aacaaagtat tttattttac cggtagtgca aaggttgctt tctccatcaa aaagttcaac
      481 atgcacggtt accaaacgtt tttcacgatt atcaattcgg cctcgaacta ccacttggcc
      541 tttaccaatt agcatcggct tactgtaaac aatctccatg ctgtgggtaa ctcccgctgt
      601 accttcaagt atgtatatgc accagcttgc tacctcgtcc attagggtcg attgaatgcc
      661 accatgcaaa acaccgtggt aaccctgata atttagctca ggtaaccaac gggtttcaac
      721 ggtttgatta gtttcatcgt accaaaagct gagctttaaa ccaataggat tcttactgga
      781 gcagcaaaaa cagccgtgct ctggcaaacc ctcgtatgga ttttttattg caaccatttc
      841 aattcaatta tttaccttga caattttaaa acaattttaa aaaacttaaa cctaaaacat
      901 atgtttattt taaacagatt ttaataattt tgagaaaaac ttaaaagagt atggatacca
      961 aacacggaaa cgcaacccgt ttaaagttga tggaaacagc tcgggagttg tttattgcaa
     1021 agggagtcga tagagttggc gtacgtgaaa ttgcatccaa agccggcgtt aatctatcgc
     1081 tcatgaacta ctactttcag agtaaggaga acttacttga gcagatcttt gagcaatcca
     1141 tcaaggataa tggacaaaaa atcaggcaaa tacttaatgc cgacatgcca cttgcagaaa
     1201 agattaaggt ttatatcaat acttacatcg atatattaac tgaagatccc ttgcttgttc
     1261 cctttgtact ttcaattatt cataggaatg ccgaacaggc atcaagacta aaagcggttc
     1321 atacccttta caacaccgaa actttttcaa accagcttaa acacgaggct gagctgggca
     1381 atattcgaaa ggttgacccg gaacagttct ttgttagcat gatctcgtta atcctcttcc
     1441 cgtttgccat taagtggcta attggttata gaatgacatt gagcaatgag caaatggctc
     1501 aattccttga ggataggcgc gaacatgttt acgacttgct gatgagtgca ctccgccctt
     1561 agtacaacaa accccacaaa ggctgtgggg tttttacttc accctaatct cttttaccac
     1621 ttcgctccca aagtactcat tgatatgggt aagcaaatcc ttcctgcgca tatgcagttc
     1681 ctgccttaga gcggctgaat ttaggcttag ggtcaatatc ccttccttaa attggatatc
     1741 ggttgtatgc atggctatat cgcggcctac aattctttcc caatcggaac ggattgatgc
     1801 ctgattaaag ttatcgttcc acttcatggc atcaatgaaa gcctttatgg cttcgcccaa
     1861 cgattttgtg ttgtgatcgc tcatgattac ctcgactcaa ccagttcaaa ctcagaatta
     1921 tcaaccctaa aaaagcgata ccccgacgtt acctttgcca aaacctcctc gagcctattc
     1981 ttgttagtat cagtaataaa tatttggcca aatccatcat ttgccaccat atcaattaac
     2041 ctatgcaccc tgccggtatc gagcttgtca aaaatatcgt cgagcagcaa aaggggttta
     2101 atgcctgcaa ccttagaaat gaaatcgaat tgtgcaagct taagtgcaat caggtaagtt
     2161 ttttgttgcc cctgagagcc ctcgttgcga atggggtatc catcaattaa aagcgtaagg
     2221 tcgtcgcggt gaattccaac cgaggtgtac tgcaaaaccc tgtccttttc gatattctcc
     2281 cgaaaaagtg tctcggcagg ggtatcgttc aggtgcgact tgtagctcaa cgaaaccttt
     2341 tcctgtccgc ccgacacctt gttatagtac tcctggagga ttccaataag ttgctccaca
     2401 aacgccttgc gttttgagtg gattaccgaa gcatactccg ctaactgtat gtcaatagcc
     2461 tcaatcaaat cgtaactaaa agcggtacgg gaataggttt ttaacaggct gttgcgttga
     2521 gcaagcaatc ggttatagcg caaaacctca tcgaggtaaa tatggtcaat ttgtgaaata
     2581 actccattca tatacttgcg gcgctcctcg ccacttccct caaccagctc attatcggca
     2641 ggcgaaacca taaccaccgg aagaaaacca atgtggtcgg ccagcctttc gtaggcttta
     2701 ccgttacgct taaatatttt cccttcgccc tgctttaacc cgcaatatat gctctcatca
     2761 acaccttggc gcgagtaaaa accctgcagt acaaaaaaat cctgtccgtg acgtatcacc
     2821 tgtaaatcgg ttgacgaaag cgaactcttg cacatggaaa ggaagtatat tgcatcgagc
     2881 aggtttgtct taccctgacc attgttcccc acaaagcagt ttatcttatt atcaaactcg
     2941 atatcaagct gatttatcga cttaaagttt atggctgtta gccttttaag atacatagta
     3001 gctaaaaatg aatatttgca aaggtaatta aaccgaaaat gtttgcctaa aattttcaga
     3061 tattacaaaa acaattactt ttgcgattgc tttgttaaaa ataaatattc attagttatg
     3121 gcagataaaa agaaacagca gacaaccgat actgtagaat caattgacaa cgctttaacc
     3181 cgtactgagc agttcattga gcaaaaccag aagagcctta ccattattgt agcagcaatt
     3241 ttagctattg tggctatcta ttttggttac aaaaaacttt acttacagcc caaggagaac
     3301 gaggctagct ctcaaatttt ccaggctcag tattactttg agcaggattc gtttcgcctg
     3361 gcactatatg gtaacgagaa cgatttaggc tttgccacta tagctgatag gtacggaatg
     3421 acccgcaccg ggaacctggc aaattactat gcaggcatct gccttaggga acttggtgaa
     3481 taccaaaagg ccattgatta cctaaaaaag tttgatgccg gcgaccaaat ggttacccca
     3541 attgcttatg gcgctattgg cgattgctat gtggaactca acaaactaaa ggatgctgcc
     3601 aagttttata ttaaggccgc caattacgag aagaatgatt ttaccacgcc aattttcctg
     3661 aaaaaggctg gaatggttta cgaggaactc aaacagtacg ataaggccct tgacctttac
     3721 aatatcatca agaatgattt tacaaagagc attgaggctc gcgatattga aaaagatatt
     3781 gcgcgtataa acacactgat gggaaagtaa agactaaaca gagttatatt taaggggtgc
     3841 ttttaaacac cccttatttt tttacaaatt cgtatctttg aaaaaaaatc ctatggcaac
     3901 atatctgaaa aacctatcga gctatgaccc taacatggtg cctagcgcaa agaacatgaa
     3961 gtttggcatt gtggtttcgg attggaacga ttctgtaacc cataagctac ttgagggtgc
     4021 ctatagcgcc ctaattgagc atggcgccac cgacgaaaac attcttgtaa agcacgttcc
     4081 gggtgccttt gagttaaccc ttggcggaca gttcatggcc gagtatgccg atttggatgc
     4141 tgttatatgc cttggctgtg ttattcaggg cgaaacccgc catttcgatt ttatttgcca
     4201 gggtgtaaca tacggaatta ccgaactcaa catgaactat aatattcctt tcatttttgg
     4261 agtgctaact accgataacc ttgaacaagc actcgaccgc gctggaggga aacacggcaa
     4321 caagggagtt gaagcagcaa tcactgccat taaaatggct gcgctccaaa aagaaatgga
     4381 ggaactggaa ggatagcgga ttcgttgttc gttgatggtt gttcgttgat ggttgttcgt
     4441 tgatggttgt tcgttgctcg tgatatttat tacctgatat ttgacctaag gtttggcatt
     4501 atacgaaata agtgtaaaaa atttgtatcc cccctttaac ggttaacggt tcacgtttaa
     4561 cggataacgg ttaacgattc acggctcacg gttcacggtt cacgaacctc ttttaacttt
     4621 tcacttttaa ctccactccc acccttctca acaaaatcag cttaaaaaat ggcacaattt
     4681 ccagctaatg ctatatttgc attaccggag ccttaaccaa ttaaaaatga aacgtgtttt
     4741 ttgggtattt tttcctttga tatttgctca aacaattagc gcacagcaaa gcaacccatt
     4801 gataagcaaa gctgattcag ccagccaaat gcgctggatc gattccgttt tcaacagcct
     4861 aagcttaagg gaacgtattg cacagctttt tatggtggct gcatactcca acaaggacga
     4921 ttcccatgta atggagattt cgcaacttgt atcaaaacaa ggaattggtg ggttgatatt
     4981 ctttcagggt ggacctgtcc gtcaagcacg gcaaactaat tactaccaat cgcttgcaaa
     5041 ggttcccctt cttattggca tcgacgccga atggggatta ggaatgcggt tggatagcac
     5101 catctcgtat cccaggcaaa tggcgcttgg tgccaccaac gataccttgc taacctacca
     5161 aatggcagcc gatattgccc atcagttcaa acgaatgggt atccatatca actttgcacc
     5221 ggttatcgat attaactcca atccgctcaa ccccgttatt ggctccagag cttttggtca
     5281 ggacatggaa atggtaacca ggcatggcat ggcttacaca aaaggactac aggaaaatgg
     5341 tattctggct tgtgccaagc attttcccgg ccatggtgac acctacgacg attcacacct
     5401 aaccctaccc ctggtgccac atagcgcaaa ccgaatcgat tccatagaac tctacccttt
     5461 caaaaagctt tttgctgctg gtgtagccag tgttatggtt gcacacctaa aaattccctc
     5521 ccttgaaccc gataccctct tgcccgccag cctatcggcc cgcattgttg gccaaaaact
     5581 ttttggcgaa cttggattta acggccttgc atttaccgat gccatgaata tgaaaggtgt
     5641 tgctgatttc tacaacccgg ttgatgccaa catacgttcc cttatggccg gcaacgacat
     5701 tctgctcttt ccaagcgaga ttgagaatac tattaaaaaa gttgaacagc ttattaaaag
     5761 cgaacaaatc cccgaagaac ttatcaacat taaatgcaaa aaggttctta tggccaagta
     5821 tctggttggt ttaaataaat accgcccaat taatgttgat aggctttata acgatttaaa
     5881 cagtccttac tcgcaggtac tccgccggaa aattatacga aacgcaatta ctgtaataca
     5941 caatgcaaat aatatccttc cccttagagg gctcgatact cttaggattg cttatattga
     6001 aattggcgaa aataaaggcg atgcattccg ggagcagctt gagctttaca ctgccgtgca
     6061 tactttctcc atcaaccccg aagccgagca ggcagagtac gataccctac ttaccaatct
     6121 agagccatac aacctaatta ttgttggtta ccacactatt gaaagcaagg ctgcaaaggg
     6181 ttttggggta agtcaacaat catcaaattt cctgttcgac ctatcgttcc gtaaaaaggt
     6241 gattcttaat ctgtttggca acccctactc cattagcaga tttgctaatc ctttagcatt
     6301 tgctgccata attgtatcgt acgataactc aaccgatagc cagaacctta cagcccaaac
     6361 catttttggt ggtattccgt tctcaggcag gttaagcgta acggcaagcg acctgtttgg
     6421 cattaacacc ggttacgcat caaaccaaag aattagactt ggatacagca tccccgagga
     6481 acttggaata gggtcggaac agctctcgga ggttgactcc cttgcaatgg aaataataag
     6541 aacaggggct gctccaggca tgcaaatcct tgcagcaaag gatggggttg tattctataa
     6601 caaggccttt gggaaaccca cctacgtttc ggagtatggc aacgatatca gaatgcttta
     6661 cgatattgca tcggttacca aggttgccgc aacgttgcca cctattatga acctgtataa
     6721 ccgaggtgtg ctgtcgttcg attcaaaact tggccagttt atcgatttaa gaaacttccc
     6781 cgataagtcg aaactacttt taggtgatat cttgaggcat caatcgggtt tacaggcctg
     6841 gattccattt tatatgaaca cattaacatc gttggaacct aacataccgt tgtggactga
     6901 aacattcagc caaactcatc cctataaata taacgggaaa cgatacgcca gccggtttgc
     6961 ctacccaagc cctaagttct accgaaccga ttcaatcagc aacttcccaa ataatgttgc
     7021 ataccgcata tactctttgg caagtatcaa cgattccatt taccggtgga ttaaccagtc
     7081 gccaatttcc gatgcgggta aataccgata tagcgatttg ggtttcctat acctgcaaaa
     7141 ggtaattgaa acagttgaac agaaaagatt agacaaggta gttgaaccgc tgtatagcaa
     7201 gttagggatg aactacacct gttttaatcc gctactacgt ttcgacaccg aaagaatagc
     7261 cccaacagaa catgaccccg ttttccgtaa acagctgata tgggggcatg tacacgatcc
     7321 ggctgctgcc atgcttggcg gagttgctgg tcatgcaggc ctttttgcca acgccaacga
     7381 tttggctaag cttatgcagc tgtacctgaa cgacggagaa tacggtggcg aacgcttttt
     7441 tgctccctca acggtaaaaa gttttgccaa cccagctaca acaaatggaa accgaagggc
     7501 ttacggcttc gataagccca atgccagcgg ttcgtcgcct gcgggaacaa tggcttcgcc
     7561 cgaaagcttt ggccacacag gttttacagg aacaatggta tgggctgacc cacaaaacgg
     7621 attggtattt attttcctat ccaaccgtgt tcaccccgat gctgaaaata ctaaactttc
     7681 agcaatgaat taccgcacac gaattcacga tgtattttac cgtgccattc aaagcattag
     7741 caagcctagt gggcaataat tgagaagagg cacaacaact gcattccaca aggtgtagaa
     7801 aattttctac acctttttgc tttttatatt ggaattcgtt tcaaactcaa attatataaa
     7861 cctgattctc ttctagttgt gattttttct taactattaa aatctattca ataattctgg
     7921 cacggttttg ttattactta tgacgtccca aacaccataa atacataaat tgctgttgcc
     7981 accagcagcc aggttgtccg gatcaccgca tttactgtcg gccatgcatc gaatccacca
     8041 ttagtcccag atctaaaaga cagtataacc cggacaactt ttttttatcc aagattcaat
     8101 gtcgttaaga ttacaaaaat atacatgcct gtcatcctga gcggagcgaa ggatcactaa
     8161 tcgaccccat aagacgtttc gcttagttca acatggcaat agtttcacga acaacaaaca
     8221 acgcttccag ccccgtaggg gcgaaacatt tgtaacccca tggctactcc cacatgtata
     8281 gcgatgcacg aaatgccttt accaaagagc aaaggtgaaa ctttgcatcg caaaagaaaa
     8341 atcattacaa agaattattt acacgttctt tcttgtcttg atacaagaaa gaaccaaaga
     8401 aaatcaaggc taaaaagccc gacccgatgg gtaccccgcg ggcggaactg ccacgcgata
     8461 ctattcgcca cgcctgcggc gtgactcatt tgatatcgct tactaggtcc accgcaacgt
     8521 tccacccccg acccattacc ggttcaggct tttatgccac tgcttgcttc tttcgctcct
     8581 tgttggttga ggaatcacat taaaaagagt tgaagcacaa agagatgctt cgacttggct
     8641 cagcatgaca aagggagtga gggagccaat ggtattgtca ttccgagcta gt
//
LOCUS       DAOSAL010000089         8522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_5422_length_8522_cov_50.799929, whole genome shotgun sequence.
ACCESSION   DAOSAL010000089 DAOSAL010000000
VERSION     DAOSAL010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 8522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8522
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5422_length_8522_cov_50.799929"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1355)
                     /gene="ftsZ"
                     /locus_tag="PK049_10210"
     CDS             complement(<1..1355)
                     /gene="ftsZ"
                     /locus_tag="PK049_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOK61972.1"
                     /translation="MGDDIINFDLPIINQSIIKVIGVGGGGGNAVNYMHRMGIKDVDF
                     VLCNTDAQALVNSPVEFKVQLGATLTEGRGAGNKPEIGRQAAIESLQQIVSLLQKNTK
                     MVFITAGMGGGTGTGAAPIIAKAAKELGILTVAIVTIPFRTEGRKRMMQAIEGIRNIS
                     QHVDSLLVINNEKLLEVYGNLKLTEAFAKADDVLATAAKGIAEIITVHGYINVDFADV
                     ETVMYNSGVALMGSGRANGQNRALEAVKQALESPLLNNNDISGARNILLNITYGQDEI
                     TMEEVGVIIDYVQHAAGDDADLIWGNGCDPSLDEDLKVTVIATGFGTNSIPELYVKER
                     NIEVISLDSEQHATKVELVNDTPEIDPIPARQTSKPKKQPVIDPAQRVIEFDVNSDVF
                     EVKTVQAAKANEIEIDIQPASPKPATPQAAPFAGTSNNKWANLDEIENVPAFKRRNMN
                     I"
     gene            complement(1419..2753)
                     /gene="ftsA"
                     /locus_tag="PK049_10215"
     CDS             complement(1419..2753)
                     /gene="ftsA"
                     /locus_tag="PK049_10215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOK61973.1"
                     /translation="MAQDNNTYVAAIDLGTTKVVTLIGKRTTNGRLQIVGFSSTDSTG
                     IKKGMIQNIEETVKAVEKTVREAESQAGVKIKSAFVGIAGQHIYSIKNYGYITLGSEE
                     TEIKKSHIKRLIEDMYNIPVDTGDSILHVLPLDFSIDNVKIEDDKPIGMLGRRLGANF
                     HIVIGKTSSAKHIEKCVDRAGVKVSELILEPLASSKAVLTDEEREAGVALIDIGGGTT
                     DLAVYYNGIVRHTAVIPIGGSIITQDIKEGCQILQKHAEMLKLHHGYALMSMAPDNKI
                     ITVPGIGGREPKEISMRSLAGIIQARMEEILEYAMYEIQMSGFANKLTAGVVITGGGA
                     LLKHIVSLIKFNWGYDVRIGYPSEMLCSESLENYNYPQYSTVIGLGLMGLEYIDAGEA
                     SPCTTTETKIEENKTEEFAEVQAKESKQINKRTLGMKITDVLSKLFAEDDIN"
     gene            complement(2759..3565)
                     /locus_tag="PK049_10220"
     CDS             complement(2759..3565)
                     /locus_tag="PK049_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61974.1"
                     /translation="MNRKLRIGLKVLAWLFVLAWVIYGLSYANKVYKGKRCTKLEVRV
                     LDSAKLNFVRAKDVYRTIVKSKNSPIGQSLNSINTNRIEQDIYQMAAVRDVQAFKTAK
                     GEVIVEITQRQPVLRVFNAKGQTYYIDDSGKILPYSSGFAANTLVANGNIVEPFKVVP
                     NLDVTAFTDSSKEGPKNFIHKLYEFAMYVNNDKFWNAQIEQVYVRNRNNIELIPLVGP
                     HIIVLGNLDNYEVKLKKLKLFYEKALPAEGWNKYTVINLKFKNQIVCTKY"
     gene            complement(3558..4955)
                     /gene="murC"
                     /locus_tag="PK049_10225"
     CDS             complement(3558..4955)
                     /gene="murC"
                     /locus_tag="PK049_10225"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOK61975.1"
                     /translation="MELNAVKRVYLLGIGGIGMSAIARYYLHYGVPVAGYDRVSSDIT
                     QNLEAAGANIHYTDSVGAIPRAFLNREETLVIYTPAIPANHSELEYFRSNRFVLMKRA
                     QVLGLLSSNYKTLAVAGTHGKTTTSTLLAHLLATSMGCDAFLGGISRNYSSNLILADQ
                     GKQLLVVEADEYDRSFLTLHPHLAIVTSVDADHLDIYGTYDAVLNAFQEFVSQIKPGG
                     YLVVKQNIAFKPIVGKETEVITYGFTPDCHYYPTNISIHDGLYRFTLVTPQGSINNLT
                     LGVPGHYNLENAIAASAAALTLGVPTEKLAHGLESFRGVQRRFDVRYRGTKIIYIDDY
                     AHHPSEIEALVRSVREVFPNRKITGIFQPHLYSRTRDFAAEFAAALDKLDKPIITEIY
                     PARELPIEGVTSETIMKLMSNSNAQVVPKAELISWLKCNNVDILLTIGAGDIDRMINE
                     IVNILEAKENRAINE"
     gene            complement(4960..6087)
                     /gene="murG"
                     /locus_tag="PK049_10230"
     CDS             complement(4960..6087)
                     /gene="murG"
                     /locus_tag="PK049_10230"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005927213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOK61976.1"
                     /translation="MEKNRLRIIISGGGTGGHIFPAISIANAIREQNPDAEILFVGAE
                     GRMEMDKVPQAGYNIVGLPVAGFSRSLTPKNLIVIIKLIKSLVKANRIVKHFKPHVVV
                     GVGGYASGPIVKAAQRKGIPTLLQEQNSYAGVTNKLLAKKAKTICVAYEGMEKYFPAQ
                     KIILTGNPVRQDIENLSVTRNQGLEHFGIGADKKVLLVLGGSLGARTINQSLMASIHL
                     IKARHDIVVIWQTGKLYYQTVKEALDSNPVENIRLYDFIARMDLAYVSANTIISRAGA
                     GTISELCLVAKPCILIPSPNVAEDHQTRNAQALVSRDAAIMIPDSQAKEQLVKAAFEL
                     LDNPAEQKKLSENIARLALRNSAITIALEVLKLAGIKKTKN"
     gene            complement(6056..7225)
                     /locus_tag="PK049_10235"
     CDS             complement(6056..7225)
                     /locus_tag="PK049_10235"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOK61977.1"
                     /translation="MGALFDKYLKGDRVIWMIVIFLYLSSLLLIYSSSSSLAFRYHGG
                     NTTYFLIKQLVFLLVAFIVMWTAHLIKYSIWYRLANILLALSVPLLGITLLFGRNLNA
                     ASRWLEIPGLGISFQSSDIAKVILIMYVAKYLSQMQENTDDFKQFLIQLIAPVAFVCA
                     LILPANFSTAALLGLTCWLMMFVGRVNLKYLFGITGIGIVLVGAFIAISLQFKTLSRA
                     QTWVNRIENFISDDKTEGNYQLEQSKIAIATGGIIGKGPGRSTQRNFLPHPYSDFIYA
                     IIIEEYGVVGGVFTLILYLMLLYRAALLVKKSNRLFPAFLAFGLALMLTLQALINMAV
                     AVGAIPVTGQPLPLVSLGGSSLIVSSGALGIILGISRSQDKKELFDGEEPVKDNN"
     gene            complement(7242..>8522)
                     /gene="murD"
                     /locus_tag="PK049_10240"
     CDS             complement(7242..>8522)
                     /gene="murD"
                     /locus_tag="PK049_10240"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005649514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOK61978.1"
                     /translation="LAQKKGFDVFLSDLSEIKPSYKEWLNKYDIRFEEGKHTEELILN
                     ADEVIKSPGIPDKAPMVVKIREKGIPIISEIEFAGRYTNAYTVCITGSNGKTTTTSLI
                     YHMMQKAGLNVGLAGNIGNSFAYQVAECSYDYYVIELSSFQLDGIYSFKPDIAILLNI
                     TPDHLDRYDYKMQNYVESKFRITRNLSEDDCFIFCSDDAVTVEQLKKIVLRAQQLPFS
                     QKTKENQTAWLENLDMFINYDNSDFSMSINDLSLKGKHNIYNSMAAGIAGHVLNIRKE
                     VIRESLSDFQGVEHRLEYILTVRGVKYINDSKATNVNSAWYALESMTTPVVWIAGGTD
                     KGNDYSELMDLVKQKVKALICLGVDNSKLHAAFDGVVPVIVDAKSAEEAVQHAYRISV
                     PGDTVLLSPACASFDLFENYEDRGRKFKAAVRAL"
BASE COUNT         2150 a         1963 c         1884 g         2525 t
ORIGIN      
        1 tcaatgttca tgttgcggcg cttaaaggct ggaacgttct caatttcatc gaggttagcc
       61 cacttattat tgctagttcc ggcaaatggt gctgcctggg gagtagcagg ttttggcgag
      121 gctggttgaa tgtctatttc aatttcgtta gcttttgcgg cctgcactgt ttttacctca
      181 aaaacatctg aattaacatc gaattcaatt accctttggg caggatctat tacaggttgt
      241 tttttgggct tgctagtttg cctggcaggg atagggtcaa tttcaggtgt gtcgttaacc
      301 agctcaacct tagtggcatg ctgttcggag tcgagcgaaa ttacctcaat attgcgctcc
      361 tttacataga gttctggtat gctgttggta ccaaagcctg tggcaatcac cgtaaccttt
      421 agatcctcat caagcgatgg gtcgcagcca ttaccccaga ttaggtcggc atcgtcgcct
      481 gcggcgtgct gaacgtaatc aatgattact ccaacctctt ccatggtaat ctcatcctgc
      541 ccataggtga tgtttagcag tatgttgcgc gctcccgata tgtcgttgtt gttgagcagg
      601 ggcgattcaa gggcttgttt aaccgcttcg agcgcgcggt tttggccatt agccctgcct
      661 gagcccatga gggcaacccc actattatac atcacggtct ccacatcggc aaaatcaaca
      721 ttgatatacc catgtacggt gatgatttcg gcaatacctt tggcggcggt tgccagaaca
      781 tcgtcggctt tggcaaatgc ctcagttagt ttcaggttgc cgtacacctc aagcaatttc
      841 tcgttgttta taaccagcag cgagtccaca tgctggctaa tattcctaat accctcaatg
      901 gcttgcatca tccttttgcg cccttccgtg cggaaaggaa tggtaacaat ggctacggtt
      961 agtatgccaa gctcctttgc cgcctttgca ataattggtg cggcacctgt tccggtacca
     1021 ccgcccatcc ctgctgtaat aaaaaccatc ttggtatttt tctgcagcag gcttacaatt
     1081 tgctgcaaac tctcaatggc agcctgacgg ccaatttcag gtttattacc agctccacgg
     1141 ccctcggtaa gggtagcgcc aagctgaacc ttaaactcta ccgggctatt gactaatgcc
     1201 tgggcatcgg tgttacaaag aacaaagtca acgtccttta tccccatgcg atgcatgtag
     1261 tttacggcat ttcctccacc tccaccaact ccaattacct ttataattga ttggtttata
     1321 attggcaggt caaaatttat tatatcgtca cccataacat tcatttttaa gtttcacaca
     1381 aacaccattt ttaatagaag gcacaagaca atagcaggtt agtttatatc atcctcggca
     1441 aagagtttgg agagcacatc ggttattttc attccaagag tacgtttatt gatttgcttt
     1501 gactcctttg cctgaacctc ggcaaattct tcggttttat tttcttcgat tttggtttcc
     1561 gtggttgtac agggtgaggc ttcgcctgcg tcgatatact ccagccccat taaacccaga
     1621 ccaataacgg tagagtattg tgggtagtta taattttcaa gcgattcgga gcataacatc
     1681 tcagaggggt aaccaatacg aacatcgtag ccccaattga attttataag cgaaacaata
     1741 tgctttagca gcgcgcctcc accggttatt actacaccgg ctgtaagctt attggcaaag
     1801 cccgacatct gaatttcgta catggcatat tcaagtatct cctccatgcg agcctgaata
     1861 attcctgcaa ggcttcgcat cgatatttcc ttgggttccc ttccgcctat tcccggaacg
     1921 gtgataattt tgttatcggg ggccatgctc atcagtgcat atccgtgatg gagctttagc
     1981 atctcggcgt gcttttgaag tatctgacaa ccctccttaa tatcctgcgt aataattgaa
     2041 ccgccaatgg gaataactgc cgtatggcgt acaatgccgt tgtagtaaac ggccaaatcg
     2101 gtggtgccac ctcctatgtc gattagggct acgcctgctt cgcgctcctc gtcggtgagc
     2161 acagccttgg atgaggctaa gggctcaagt ataagttccg atacctttac ccctgcacga
     2221 tccacacact tttcaatgtg ctttgccgat gaggttttac caattactat gtggaagttg
     2281 gcgccaagcc ttcggcctag catgcctatg ggtttatcat cctctatctt tacattatcg
     2341 attgagaaat ccagcggcaa tacatgaaga atgctatcgc ctgtatcaac agggatatta
     2401 tacatatcct caataagacg cttgatgtgg ctctttttga tctcagtttc ttcggaaccc
     2461 agcgtgatgt agccatagtt tttaatgctg tagatgtgtt gccctgctat tccaacaaag
     2521 gccgatttga ttttaacgcc agcctgcgat tcggcctcgc gaacggtttt ttcaacggcc
     2581 tttactgttt cctcaatgtt ttgaatcatt ccctttttta taccagtgga atcggtggag
     2641 ctaaagccca caatctgcaa gcgtccgttt gtggtgcgtt tccctattaa ggtaactacc
     2701 ttggttgtac caaggtcaat tgcagctacg taagtgttgt tgtcctgtgc catagttgtt
     2761 agtattttgt gcaaactatt tgatttttaa attttaggtt aattacggtg tatttgttcc
     2821 agccttcggc tggcaacgct ttctcgtaaa agagtttaag tttcttgagc ttaacctcgt
     2881 agttatccaa attgcccagt acaatgatat ggggcccaac caagggaata agctcaatgt
     2941 tattcctgtt cctaacgtaa acctgttcta tttgagcatt ccagaacttg tcgttgttta
     3001 catacattgc aaactcgtaa agcttgtgta taaagttttt tggaccttcc tttgagctat
     3061 cggtaaatgc tgtaacatca aggttgggca caaccttaaa gggctccacg atattaccgt
     3121 ttgccaccag cgtgttagca gcaaaccccg atgagtaggg tagtatttta ccagaatcgt
     3181 cgatatagta ggtttgccct ttagcattga aaactcttaa aacgggttgg cgttgagtaa
     3241 tttcaactat tacttcgcct ttagcggttt taaatgcttg aacatcgcgc acagcagcca
     3301 tttggtaaat atcctgttca attctattgg tatttatgct gttaaggctc tgtcctatag
     3361 gtgagttttt tgattttaca attgttctgt atacatcctt agccctcaca aagttaagtt
     3421 ttgcgctatc gagaactctt acctcaagtt ttgtgcagcg tttcccttta tacactttat
     3481 tggcatagga aagcccataa ataacccagg ccagaacaaa aagccaagcc aaaactttaa
     3541 gccctatgcg caacttccta ttcatttata gctctattct cctttgcttc aagtatgtta
     3601 actatctcgt ttatcattct gtcgatatcg cctgcaccaa tggtgaggag aatatccaca
     3661 ttattgcatt ttaaccagct aattaattcg gcctttggaa caacttgcgc attactgtta
     3721 ctcatcagct tcattatggt ttccgatgta accccttcaa ttggaagttc ccttgccgga
     3781 tatatttcgg tgataattgg tttatcaagc ttgtcgagtg ccgcagcaaa ctccgctgca
     3841 aagtcgcggg tgcggctgta gaggtgaggt tgaaatatac cagtgatttt tcggttaggg
     3901 aaaacctcac gaaccgatct taccaacgct tcaatttcgc tgggatggtg tgcataatcg
     3961 tcaatgtaga tgatttttgt tcccctatac cttacatcaa agcggcgttg aacaccccta
     4021 aacgactcta agccgtgagc taacttttca gtcggaacgc caagggttaa cgctgctgcc
     4081 gatgcagcaa ttgcattctc caggttgtaa tggcctggta ctcctagtgt aagattgttt
     4141 attgaaccct ggggagttac aagggtaaac ctgtaaagtc catcgtggat gctaatgttt
     4201 gttgggtagt aatggcagtc gggcgtaaat ccgtaagtaa tcacttcagt ttccttgccc
     4261 acaataggtt tgaatgcaat attttgcttt acaaccaaat acccgccggg ttttatttgc
     4321 gacacaaatt cctgaaaagc attaagtact gcatcgtaag tgccgtaaat gtcaaggtgg
     4381 tcagcatcaa ccgaggtaac aatggcaaga tgtgggtgaa gtgtaaggaa gctacggtcg
     4441 tactcatcgg cctctaccac aagtagctgc ttgccttggt cggcaagtat caggttgctg
     4501 gagtagttgc gcgatatgcc tcccaaaaag gcatcgcaac ccattgatgt agccaaaagg
     4561 tgggccaaca gggttgatgt agtagttttt ccgtgagtgc ccgcaacggc aagggtttta
     4621 tagtttgaac ttagtaatcc aagaacctgt gcgcgtttca tcagtacaaa cctattgctg
     4681 cggaaatatt caagctcgct gtggtttgca ggaatagctg gtgtatagat aacaagagtt
     4741 tcttccctgt tcaggaatgc tcttggaata gccccaacgc tatcggtgta atggatattt
     4801 gcccctgcag cttcaaggtt ttgggtaata tcggacgaaa ccctgtcgta ccctgcaaca
     4861 ggaaccccgt agtgcaagta gtaacgggca atggcactca ttccaatccc tccaatccca
     4921 agtaggtaaa ccctttttac tgcattcagc tccatcggtt caattttttg tttttttaat
     4981 acctgccagt tttaaaactt caagggctat agttatagcc gagtttctta gtgccaaacg
     5041 ggcaatgttt tccgatagtt ttttttgttc cgcagggttg tcaagcagtt caaaggctgc
     5101 tttaaccagt tgctcttttg cctggctatc gggtatcata atggctgcat cgcgcgaaac
     5161 cagtgcctgg gcgttacggg tttggtggtc ttcggcaaca ttgggcgatg gtataagtat
     5221 gcaaggtttc gcaaccaggc agagttcgga aattgttcca gcacctgctc gagaaataat
     5281 ggtgttagct gaaacatagg ccaagtccat gcgggcaatg aaatcgtaca gcctaatatt
     5341 ttcaaccgga ttgctgtcaa gtgcctcttt tactgtttgg tagtaaagtt tcccggtttg
     5401 ccatatcacc acaatatcgt gtcgggcttt tattaggtgg atcgaagcca tcaggctttg
     5461 gttaatagtt cgggctccaa ggcttccgcc taaaacgagt aaaacctttt tgtcggctcc
     5521 aatgccaaaa tgttcaagtc cctgattgcg tgtaacggaa aggttttcaa tatcctgccg
     5581 aacaggattg ccggttaata ttattttttg tgcagggaag tatttttcca tgccttcgta
     5641 ggctacacag atagttttag cctttttggc taaaagctta ttggtaaccc cggcgtaaga
     5701 attctgctcc tgtagtagcg tagggatacc tttgcgctgt gctgccttta caataggtcc
     5761 gctggcatat cctcctacac caaccacaac gtgaggttta aaatgcttaa ctatgcggtt
     5821 tgcttttact aagctcttaa tcagcttaat aatcacaatg agattttttg gggtaaggct
     5881 tcgtgagaaa ccggcaacag gcaagcctac aatgttgtat ccggcttgtg gcaccttatc
     5941 catttccatt cggccctcgg ctccaacaaa cagaatctca gcatcggggt tttgctcccg
     6001 aatggcattg gctatggaaa tggcagggaa tatatgcccg ccagtacccc caccactaat
     6061 tattatcctt aaccggttct tctccatcga atagttcttt tttatcttga cttcgactaa
     6121 tacccagtat aatacctagt gcaccactgc taactatcag cgatgaacct cccaaactta
     6181 caagtggtaa tggctgaccc gtaacgggaa tggctccaac cgcaacggcc atattaatta
     6241 atgcttgcag cgtaagcatt aaggccaatc caaaggcaag gaatgctgga aatagccggt
     6301 tcgatttttt aaccagcagg gctgcccggt aaagtagcat aagatagagt attagggtaa
     6361 atacaccgcc taccacacca tactcctcaa taattattgc gtaaataaag tcggaatagg
     6421 ggtgtggcaa aaagttacgc tgggttgagc gtccaggccc tttaccaata atacccccag
     6481 ttgcaatggc tattttcgat tgctccagct gatagtttcc ctctgttttg tcgtcgctaa
     6541 taaaattctc aatgcggttt acccaggttt gggctctgct cagggtttta aactgaagcg
     6601 atatggcaat aaaggctccc accagtacaa tgcctatccc agtaatccca aagaggtatt
     6661 ttaggttaac tcgccctaca aacatcatta accaacaggt taagccaagc aaagctgcgg
     6721 ttgagaagtt tgccggtaaa attagagcgc acacaaatgc tacgggtgca attagctgga
     6781 ttaggaattg tttaaaatcg tcagtgtttt cctgcatttg cgagaggtac ttggccacat
     6841 acattattaa aatcaccttt gctatgtccg atgactgaaa cgaaatgccc aatccgggta
     6901 tctccagcca acgcgatgca gcattgaggt tacgtccaaa aagaagtgta ataccgagca
     6961 ggggcactga caaagccaga agtatattgg ccagcctgta ccatatgctg tattttatga
     7021 gatgcgcagt ccacatcact atgaatgcaa ccagcaggaa aacaagctgt ttaatcagga
     7081 aataggttgt gtttccacca tgatagcgga aagctagtga gctcgatgag ctgtagatga
     7141 gcaggagcga tgaaaggtaa aggaagataa caatcatcca aatcacccta tcgcccttta
     7201 agtatttatc gaacagtgct cccatagctc actgggttat attaaagcgc gcgaacagcc
     7261 gctttgaact tgcgtccacg atcctcatag ttctcaaaca ggtcaaagct tgcacaggct
     7321 ggggaaagca gtaccgtgtc gcccggaacc gatattctgt aggcatgctg cacagcctcc
     7381 tcggccgatt tggcatcaac aattacggga acaacgccat cgaatgctgc gtgaagcttt
     7441 gagttatcca ctcccaggca gattaaggct ttcaccttct gctttactaa atccataagc
     7501 tcggagtaat cgttcccttt atcagtaccg cctgcaatcc aaaccacggg ggttgtcatg
     7561 ctctccagag cataccatgc ggagttcaca ttggtagcct ttgagtcgtt aatgtactta
     7621 acacctcgca cagttagtat atattccaac ctatgctcta ctccctgaaa atcggaaagc
     7681 gattcacgaa taacctcttt acggatattt agtacatgac cagcaatacc ggcagccatg
     7741 gagttgtaaa tgttatgctt ccctttcagg gagaggtcgt ttattgacat gctgaaatcg
     7801 ctgttatcgt aatttatgaa catatcgagg ttttcgagcc atgcggtttg gttctccttt
     7861 gttttctgtg agaagggcaa ttgctgggcg cgtagtacaa tctttttgag ttgctccacg
     7921 gttacagcat cgtcggagca gaaaatgaag cagtcgtctt ccgagaggtt tcgggtaatc
     7981 ctgaactttg attccacata gttctgcatc ttgtagtcgt acctgtccag gtggtcgggg
     8041 gtgatgttaa gcagtatggc gatatcgggt ttaaagctat agattccatc gagctgaaag
     8101 ctgcttagct caataacgta gtagtcgtag ctgcattcgg caacctggta agcaaaacta
     8161 ttgccaatat taccagccaa cccaacgttt agaccagctt tctgcatcat gtggtaaata
     8221 agcgaggtgg tagtggtttt gccgttgcta ccagtaatac aaacggtgta ggcgttggtg
     8281 tatcggccag caaactcaat ctccgaaatt atggggatgc ccttttccct gattttaaca
     8341 accattgggg ctttgtcagg aatccccggg cttttaatca cttcgtcggc attgaggatt
     8401 agctcctcgg tatgtttgcc ctcctcgaag cggatatcgt acttgtttaa ccattccttg
     8461 tagctgggtt taatttccga taaatccgat aggaatacat cgaatccctt tttctgggct
     8521 aa
//
LOCUS       DAOSAL010000090         8445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_5479_length_8445_cov_49.705721, whole genome shotgun sequence.
ACCESSION   DAOSAL010000090 DAOSAL010000000
VERSION     DAOSAL010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 8445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8445
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5479_length_8445_cov_49.705721"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..331
                     /locus_tag="PK049_10245"
     CDS             149..331
                     /locus_tag="PK049_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61979.1"
                     /translation="MGTPRVELPRDTIRHACGVTHVVSLTRPTATFHPRPISGSGFYA
                     IACFFLSIPSQGKRLG"
     gene            complement(589..774)
                     /locus_tag="PK049_10250"
     CDS             complement(589..774)
                     /locus_tag="PK049_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61980.1"
                     /translation="MAKNEIHCNGAAGTLYGLGFIGAAIYYISAATSFWVGVLGFLKA
                     LVWPALLVYGLLKHFGL"
     gene            complement(842..1615)
                     /locus_tag="PK049_10255"
     CDS             complement(842..1615)
                     /locus_tag="PK049_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOK61981.1"
                     /translation="MKRILYTILSLLALTVPVKAQDIPDPMSPPRLVNDFAGILSADE
                     QNALEQKLRAYNDTTSTQIYVVTVKDLNGYEPSDFAFRLGEKWGVGQKGKNNGIVILI
                     KPKIGNDKGRAFIATGYGMEEVIPDVTARQIVDKEMIPYFKENRYYKGIDAAINVIID
                     LAAGLYTADEYYSGESPWPGIIFIIIFFAIIFLSARRGNRLQGGSIGRSLPFWLLMSS
                     MGSGRGSSFGHFSSGSGSFGGFSGGGGGSFGGGGAGGSW"
     gene            complement(1631..2068)
                     /locus_tag="PK049_10260"
     CDS             complement(1631..2068)
                     /locus_tag="PK049_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOK61982.1"
                     /translation="MKPKDYFTPAQQGQIVEAIEQAERQTSGEIRVHVEWECKGSPLD
                     RAARIFSMLKMHKTKLRNGVLFYLSLKDHKFAILGDVGINVKVPENFWDTIKEAMLAE
                     FKEGRLTEGLVKGIGMAGEQLSAHFPYDPNTDTNELSDEISFG"
     gene            complement(2084..2686)
                     /locus_tag="PK049_10265"
     CDS             complement(2084..2686)
                     /locus_tag="PK049_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOK61983.1"
                     /translation="MNISKKWIVAGIVILLVVFIYSSIKGTYNNMVKKDEGVKAQWGN
                     VETAYQRRLDLIPNLVNTVKGYANFEQQTLTQVIEARAKATSVNIKPDNLNEQSLKQF
                     QAAQGELSSALARLMVVVEQYPNLKANEQFVNLQAQLEGTENRIAVERRKFNELVQDY
                     NSYIRQFPKNIWASLFGFEKKGYFEAEAGAEKAPKVDFTN"
     gene            3170..6907
                     /gene="cas9"
                     /locus_tag="PK049_10270"
     CDS             3170..6907
                     /gene="cas9"
                     /locus_tag="PK049_10270"
                     /inference="COORDINATES: protein motif:HMM:TIGR01865.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HOK61984.1"
                     /translation="MKRILGLDLGSASIGWALISESENNGNVNREILGLGCRIIPYEG
                     TEGKDFEKGTGESRNSIRTKMRTVRKGYDRYQLRRKYLVQELIKHNMYPTDELKGLPK
                     MQLWELRSKAVSEKVSLPELGRILLWLNQKRGYKSSRSDSNLDKKDTEYVAKVKSRHQ
                     LIKEQNLTIGQYFYQQLKNDPFFRVKENVFPREAYIEEFDSICNEQKKHYPDILTDEL
                     ISKIRDEIIYYQRPLKSQKGLVSVCEFEGFWRKGNDGKEYFVGPKVAPKSSPLFQLAK
                     IWENINNIRIENKQGNNVEITLEQKKALFEHLDNNEKLTPTDIIRILGLKKNECFFNK
                     QLAKGINGNTTKCAILNCFDDVSKYAHLLKFDVEVIETGKQTYLYDNSTGEVINEKTI
                     KYIHPKIEKEPLYVLWHTIYSISDQDECSRVLQAKFNIEKEIADKLSGIDFTKQGFGN
                     KSAKTLRKILPYLMEGDGYSEAMCYAGYNHSNSLTKDENLKRELLDKLKPIPKNSLRQ
                     PIVEKILNQMVNVVNAIIDKYGKPDEIRVELARELKQSKDERNETYLNITKRERENEQ
                     IAKKLQEFGLRATRNNILKWRLYEEMDNEDKKLNAICIYCGQPISLVEAIKGTDVDIE
                     HIIPKSKLFDDSQSNKTLAHRHCNSGKGDMTAYDFMKSKPTEVFNAYIERVSKLYANK
                     VISKAKRDKLLMTESKIPENFIDRQLRESQYIAKKAREVLLSISHNVWTTSGSVTAEL
                     RRLWGWDDVTMNLQFNKYKELGLTSFVEWESEHGKNKHRKEVITGWSKRDDHRHHAVD
                     ALVIACTKQGYIQRFNTLNSSKTKQDLIADVEKKSLEFKEKLSLLEKYIISEQPFTVK
                     EVEEKVAEILVSFKAGKKVAVIGKRKVGKKGNKKVVQTGIIIPRGALHEESVYGKVKT
                     VERKPIKYAFENPHLIVLPKIKKLVEERLSKHENVKDAIASLKKEPLYIDKNKQVLLE
                     YASCFSSDYVIKYPVDINFNKVDKVVDGRIKQILQARLNKFNGKPKEAFKDIQRGDKV
                     IKWYEDEGLPIPIKTVRCYTGLSAVVPVKKDENGNNIGYVKPGNNHHIAIYTDSDGNK
                     HEHVCTFWHAVERKKFGFPVIIKNTNEVWDKIQEKPDGTYPEEFLEKLPSPNLTLELS
                     MQQNEMFVLGMAPEEFSDCMVKKDFKAISNKLYRVQKLAEKNYVFRHHLETQLIDDDN
                     SKRSKRYYLIQSLGALFNLNPVKVKIDYIGNITAWNNTDRV"
     gene            6951..7628
                     /locus_tag="PK049_10275"
     CDS             6951..7628
                     /locus_tag="PK049_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61985.1"
                     /translation="MPNSWDIPLEHGGYYHIYNRGINGTPLFYVPDNYLHFLRLYDKY
                     ITPIADTFAWCLMGNHFHLLVRVKEENEIGFLKDFNPSRSIDSGRLKWQFTHNLPESE
                     GSGSVNGKKPVPYRMFSHLFNSYTKYFNLKNNRTGSLFEKNFHRLKITSEKYFQNLVV
                     YIHQNPVHHGFTSDYRDYPWTSYGSIISTKSTKVHRETVLEWFGSKENFIDCHCKIVD
                     LDIIIDL"
     gene            7667..>8445
                     /gene="cas1"
                     /locus_tag="PK049_10280"
     CDS             7667..>8445
                     /gene="cas1"
                     /locus_tag="PK049_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HOK61986.1"
                     /translation="MIKRTLYFGNPAYLKAKDEQMVVEYPSGDTESKSIPIEDIGVVI
                     LDSPQITITHVLLNKLLANNAAVITTNERHMPSGLLYPLESNTLQSERFTAQLEASVP
                     LKKQLWQQTVQAKIQNQAALLKSLGIDVEPMLYWARSVRSGDPDNYEGRAAAYYWKNL
                     FSGYFNDIFTRGRYEDEPNNLLNYGYAILRAVIARSLVASGLLPTLGIHHHNRYNAFC
                     LADDIMEPYRPFVDRVVLEIVKSGIDCTELTREIKAKLLVIP"
BASE COUNT         2704 a         1691 c         1777 g         2273 t
ORIGIN      
        1 tttgcgatgc acgcaacaac ttttccaaag agcaattcag atgcgttgca tcgcaacggt
       61 aatgcattgc aaagagccat ttacacgttc tttcttgtct tgatacaaga aagaaccaaa
      121 gaaaatcaag gctgaaaagc ccgacccgat gggtaccccg cgggtggaac tgccacgcga
      181 tacaattcgc cacgcctgcg gcgtgactca tgtggtatcg cttactagac ccaccgcaac
      241 gttccacccc cgacccatta gcgggtctgg cttttatgcc attgcctgct tcttcctctc
      301 cattccctct caggggaaaa ggctggggtg aggtcgtgtc ccacgaataa cgaacagctt
      361 acacgaacaa cttcatttgt catcctgagc gcagcgaagg atctctattc atccctcgtt
      421 gttacgtttc agcggagtct atacctattt atatcgggac ccaatattac aaacaacctt
      481 aactaaacga cgttggttcc agatttctaa aagttaatac agaatattaa aaaaaactgc
      541 acgcccaaag gcatgcaggg ttaatttcgt ctaaataaca tacaaattct aaagcccgaa
      601 atgcttgagt aatccgtata ccagcaatgc aggccaaacc aacgccttta ggaaccccag
      661 tacacctacc cagaacgatg ttgctgcgga gatgtagtaa attgccgcac caataaaacc
      721 tagcccgtaa agggttccgg cagcgccgtt gcagtggatt tcattctttg ccatgatgat
      781 tttcttttaa tttaacaata ttaatcaagc tacttgttta ttggctatca accttttttt
      841 gttaccagct accacctgcg ccgccgccgc caaagctgcc gccaccacca ccgctaaatc
      901 cgccaaaact acctgacccc gacgagaagt gaccaaagct actacctctg ccactaccca
      961 tgctactcat caacagccaa aatggcagac tccttcctat actgccaccc tgtagcctat
     1021 tacccctacg tgccgatagg aagataatgg caaaaaagat tatgatgaat atgatgccag
     1081 gccaaggcga ttcgccgctg tagtactcat cggcggtgta aagccccgct gccaaatcaa
     1141 taatcacgtt aatggcagca tcaatccctt tgtagtaacg gttctcctta aagtaaggaa
     1201 tcatctcctt gtctacaatc tgtcgggctg ttacatcggg tattacctcc tccatgccat
     1261 agcctgtggc tatgaatgca cggcctttgt cgttaccaat cttaggttta atcagaataa
     1321 ctattccgtt gttctttccc ttttggccaa caccccactt ttcgccaagt ctaaaagcaa
     1381 aatccgaagg ttcgtaaccg ttgaggtcct taacggttac cacatagatt tgggttgatg
     1441 tagtgtcgtt ataggcccgc agcttctgct caagggcgtt ctgctcatca gccgaaagga
     1501 taccggcaaa atcgtttacc agccggggtg gcgacatggg gtcggggata tcctgtgcct
     1561 ttacaggcac tgtaagagcc aacaacgaga gtatggtata aagtattcgc ttcatattaa
     1621 aatcattttt ttaaccaaac gatatttcat cgctcagctc gttggtatcg gtgttggggt
     1681 catacgggaa gtgagccgat agctgctcgc cagccattcc aattccctta accaaaccct
     1741 cggtaaggcg accctcctta aactcggcca gcatggcttc ctttatagtg tcccaaaagt
     1801 tttcgggcac ttttacgttt atgcccacat cgcccagtat ggcaaacttg tggtccttga
     1861 gcgataggta aaatagaacc ccattgcgta gctttgtctt gtgcattttg agcatggaaa
     1921 aaatacgggc agccctatcc agtgggctac ccttgcattc ccactccacg tgcaccctga
     1981 tttcgcctga ggtttgcctt tcggcttgct caatggcttc aacaatttga ccttgctggg
     2041 caggggtgaa gtaatccttg ggtttcatgg ttcaaaattg atgctagttg gtgaaatcaa
     2101 cctttggagc cttttcggca ccagcttcag cctcaaagta acccttcttc tcaaagccaa
     2161 acaggctagc ccatatattt ttggggaact ggcggatgta cgagttgtaa tcctgaacca
     2221 actcattaaa cttacgacgc tcaacggcaa tacggttctc cgtgccttca agctgagctt
     2281 gcaggttaac gaactgctca ttggctttta ggttagggta ctgctcaacc acaaccatta
     2341 agcgagccaa ggcactgctt aactcaccct gagctgcctg gaactgcttt aagctttgct
     2401 cgttcaggtt atcgggcttg atgtttacgc ttgtggcttt tgctctggcc tcaattacct
     2461 gggtaagggt ttgctgctca aagttggcat agcccttaac cgtatttacc aggttgggga
     2521 taagatcgag cctgcgctgg taggctgttt ccacattgcc ccactgcgct tttacaccct
     2581 catccttttt taccatgttg ttgtatgtgc ccttaatgga gctgtaaata aagacaacca
     2641 gcaggataac aatacctgcc acaatccatt tcttgctgat gttcattgct gtatggtttt
     2701 taaggttaat aattccgatg tcattggtat caaaaatcat gcacaagtta aggtaaagat
     2761 ttaaaactgg tcacaaatac ctgtacgttt taacatatat tgggaaaata actaccaatg
     2821 caaaaaatga catactggca gaatgtgcaa ttttaaaaat ttagaatgat atgattttat
     2881 gtctttttgt ttattaagat ttttaatatt tttatgttaa gcatttaaag cggaaacgga
     2941 aaaagaaaag ggcataaaaa agaaaggcga gttgccccgc cttaaatgtt ttgttcacgt
     3001 ttttattgct tttcaatgga gttcacgaaa catttaaagc ccaaaaagaa aacgatccca
     3061 gtttgcactg agaccgcttt aaatgttttc acaacggaat aatccttatt gtgaattgct
     3121 tcagattgta atacaaatat atgctaatta ggttaaaaat acaaatgtta tgaaaagaat
     3181 tttaggactt gatttaggtt ccgcttcaat tggctgggcg ttaataagtg aaagcgaaaa
     3241 taatggtaat gtaaatcgcg aaattttggg tttaggttgt cgcattattc cttacgaagg
     3301 aactgaaggg aaagactttg aaaagggaac gggtgaaagt agaaactcta tccgaacaaa
     3361 aatgcgaacc gtacgcaagg gttatgacag gtatcaactc cgcagaaaat accttgtcca
     3421 agagttgata aagcataata tgtatccaac ggatgagctg aaagggcttc ccaaaatgca
     3481 gctctgggaa ctaaggagta aagcagtaag cgaaaaggta tcgcttcctg agttaggcag
     3541 aattctattg tggctgaatc aaaagcgtgg gtacaagagt agtagaagcg actctaattt
     3601 ggataaaaag gatactgagt atgtagcaaa ggttaaaagt cgccatcagc tgattaaaga
     3661 gcaaaacctt accattggcc agtactttta ccagcaatta aagaatgacc cgttttttag
     3721 agttaaagag aatgtattcc ctcgagaagc ctatattgaa gagtttgata gtatttgcaa
     3781 tgaacaaaaa aagcattatc ccgatatttt aaccgatgag ttaatctcaa aaattcggga
     3841 cgaaataata tattatcaaa gacccttaaa atcccagaaa ggtcttgtgt cggtatgtga
     3901 gtttgaggga ttctggcgta aagggaacga tggcaaagaa tactttgttg gtccaaaggt
     3961 tgctcccaaa agttcacctc tttttcaact tgcaaagatt tgggagaaca tcaataacat
     4021 tagaattgaa aataaacagg gaaataacgt tgaaataacc cttgaacaga aaaaggcgct
     4081 ttttgaacat ttagacaata atgaaaaact tacccctact gatattataa ggattctagg
     4141 cttgaagaaa aacgaatgtt ttttcaataa gcaattggct aagggaatta atggtaatac
     4201 aaccaagtgt gctatattaa attgttttga tgatgttagt aagtatgctc atcttttaaa
     4261 gtttgatgtt gaggttattg aaactggtaa acaaacatac ctttatgata attccaccgg
     4321 ggaggtaata aacgagaaaa ctataaagta cattcatcca aaaatagaga aagagccact
     4381 gtatgtgcta tggcatacca tatactcaat atctgaccaa gatgaatgca gtagggtttt
     4441 acaggccaag tttaacatag aaaaagaaat agctgataag ctctcaggta tcgattttac
     4501 aaaacaagga tttggcaata aatcggcaaa aacattaaga aaaattttgc catacctaat
     4561 ggagggagat gggtatagcg aagccatgtg ctatgctggt tataatcatt caaattccct
     4621 tacaaaagat gagaatttaa aaagagagtt actggataaa ttaaagccta ttccaaaaaa
     4681 ttcgctcagg caacccattg tggaaaaaat actgaaccaa atggttaatg ttgtcaatgc
     4741 aattattgat aaatacggga aacccgatga gattagggtt gaattagcca gagagctaaa
     4801 gcaaagtaag gatgaacgta acgaaactta tttgaatatt acaaagcggg aaagggagaa
     4861 tgaacaaata gctaagaaac tgcaagagtt tgggctaaga gcaacccgaa acaatattct
     4921 taaatggcgt ctttatgagg aaatggacaa tgaggataag aagttaaacg caatttgtat
     4981 ttactgtggc caaccaatct ctttggttga ggcaattaag ggcactgacg ttgatattga
     5041 acatataatt ccaaaatcca aactctttga cgattcacaa agcaataaaa cccttgcaca
     5101 tcgccactgc aattctggta aaggcgatat gactgcttac gattttatga aatcaaaacc
     5161 cactgaggtt tttaatgcct acattgagag ggtatcgaag ctgtacgcaa ataaagttat
     5221 tagcaaggct aagcgcgaca aactgcttat gaccgaaagc aaaataccag aaaactttat
     5281 tgaccgacag ttaagggaaa gccagtatat tgcaaaaaag gcaagggaag tacttctatc
     5341 aatttcacat aatgtatgga ctacttcagg tagcgttact gctgaactca ggagattatg
     5401 gggttgggac gatgttacta tgaatcttca atttaacaaa tataaggagt taggcttgac
     5461 atcgtttgtg gagtgggaaa gcgagcatgg caagaataag cacaggaagg aagttattac
     5521 aggatggagt aaacgcgacg accatcggca tcacgcagta gatgcacttg ttattgcatg
     5581 cacaaaacag ggttatattc agcgatttaa cacccttaac tcaagcaaaa caaaacaaga
     5641 cctgatagcc gatgttgaga aaaaatcttt agaatttaaa gaaaagctct cgctccttga
     5701 aaaatatata attagcgaac agccctttac agttaaggag gttgaggaaa aagttgcaga
     5761 aattttggta tcgtttaagg caggcaaaaa ggttgcggtt attggtaaaa ggaaggtcgg
     5821 gaaaaagggt aataaaaagg ttgttcaaac aggcattatt attccaagag gagcattaca
     5881 cgaagagagt gtgtatggga aggttaagac tgttgaacgc aagccaatta agtacgcgtt
     5941 tgaaaacccc catttaattg tgcttccaaa aataaaaaaa ctagttgagg aacgtctttc
     6001 aaaacatgaa aacgtaaagg atgccattgc ctcgttgaaa aaagagcctt tgtatattga
     6061 taaaaataaa caggttttgc tagaatacgc ttcatgcttt agcagcgact atgtgataaa
     6121 atatccggtt gatataaatt tcaataaggt tgataaagtt gttgatggga ggattaaaca
     6181 gatactgcaa gcaaggctta acaaatttaa tggtaaaccg aaagaggctt ttaaggacat
     6241 acagcgtggc gataaggtga ttaaatggta tgaggatgaa ggcttaccaa taccaatcaa
     6301 gacagttcga tgctatacgg gtctctcggc ggtagttcca gtaaaaaagg acgagaatgg
     6361 aaacaacatt ggttacgtaa agcccggaaa caatcatcac attgcaatat ataccgattc
     6421 cgatggtaac aagcatgaac acgtatgcac attctggcat gccgttgagc gaaagaaatt
     6481 cgggttccct gtaattatca aaaatacaaa tgaggtttgg gataaaatcc aggaaaaacc
     6541 tgatggaact taccccgaag agttccttga aaaactacca tcgcctaacc tgaccctgga
     6601 gttgagcatg cagcagaatg agatgtttgt ccttggcatg gctcctgagg aattttctga
     6661 ctgtatggtt aaaaaagatt ttaaagcaat aagcaataaa ttatatagag tacagaaact
     6721 tgcagagaaa aattacgtgt ttaggcatca tttagaaaca cagctaatag acgacgacaa
     6781 ctcaaaaaga agtaaaagat actatttaat tcagagttta ggggctttgt tcaatttgaa
     6841 tccagtaaaa gttaaaattg attatattgg aaatattacc gcttggaata acactgacag
     6901 ggtttaaaac cctgtcagtg ttatcaatga agaaataatt aaaaaacctc atgccaaatt
     6961 cttgggatat accattggag cacggcggtt actaccacat ttacaaccgt ggaataaacg
     7021 gcacgccatt gttttatgta cctgataact acttgcattt tcttaggctt tacgataaat
     7081 atatcactcc aattgccgat acctttgcct ggtgcttgat ggggaaccat tttcatttgc
     7141 tggtgcgggt aaaagaggag aacgaaattg gattccttaa ggactttaac ccttccaggt
     7201 ctatcgactc tggaaggtta aagtggcaat tcacccataa ccttccagaa tccgaaggat
     7261 ctggaagcgt taatggaaag aaacccgttc cttacagaat gttctcgcat ctgttcaact
     7321 cttatacaaa gtatttcaac ctaaaaaata atcgcacggg aagtctattt gagaaaaatt
     7381 tccatcgtct aaaaataacc tccgagaaat acttccaaaa ccttgtagtt tatatacatc
     7441 agaatcccgt tcaccacggc tttaccagtg attataggga ttacccatgg acatcgtatg
     7501 gtagtattat ctccacaaag tcaacaaagg ttcataggga aaccgtgctt gaatggttcg
     7561 gcagcaagga gaattttatt gattgtcatt gtaaaatagt tgacctagac ataattattg
     7621 acctctgaca ggttttaaac cctgtcagag gtataatgaa acaaatatga taaaacgcac
     7681 cctttatttc ggaaaccctg cctacctaaa ggcaaaggat gagcaaatgg tagttgaata
     7741 cccaagtggt gatactgaga gcaaatcaat ccccattgaa gatattggag tggttattct
     7801 tgattcccca caaattacca taacccacgt gttgctcaac aaactactgg caaataatgc
     7861 tgcagttatt acaaccaatg agcggcatat gccctcgggg ctgctttacc ctctggaatc
     7921 gaacacactg caaagcgaac gttttactgc gcagctggag gcaagtgtcc cgctaaaaaa
     7981 gcagctttgg cagcaaacgg tacaagccaa aatacagaat caggcagcgc ttttaaaatc
     8041 gcttggcatt gatgttgagc ctatgctata ctgggctaga agtgtaagga gtggcgaccc
     8101 cgataactac gagggccgtg ctgctgctta ctattggaaa aacctttttt cgggctactt
     8161 caacgatatt tttacacgtg gcaggtatga ggacgagccc aataatttgt tgaactacgg
     8221 ttatgccatc ctacgtgctg taatagcacg tagcctggta gcaagcggtt tgctaccaac
     8281 gctgggaatt caccatcaca accgctacaa cgccttttgc cttgccgacg acataatgga
     8341 accctaccgg ccgtttgtgg atagggttgt gcttgaaatt gtaaaaagtg gtatcgattg
     8401 taccgagtta accagggaga taaaggcaaa actccttgta attcc
//
LOCUS       DAOSAL010000091         8141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_5738_length_8141_cov_45.327109, whole genome shotgun sequence.
ACCESSION   DAOSAL010000091 DAOSAL010000000
VERSION     DAOSAL010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 8141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8141
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5738_length_8141_cov_45.327109"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="PK049_10285"
     CDS             complement(<1..962)
                     /locus_tag="PK049_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK61987.1"
                     /translation="MKLLRKSIVLLLSLIAGTLYAQEDVAKFLKGSLNDAEKLSKAYL
                     EPFGKSLGTSLNSGWYNSANPHGILGFDITFTVPITIPPSSDKTFDVSKLNLEYWQVK
                     SGSSSTSPTVTGKSSNSILTDKATGLSELNMPDGANLKFVPAPMIQVAKGLPFHTEIV
                     GRFLPTINISSVGNFGLWGVGVKNEFKEFIPFVKRLPFSMSVLFGYTQFASSFDINKA
                     QKQKLNFKSSGYTARLLVSKSIPVLTVYGGLGYNHSTTDISLKGTYNVEGVGTVTDPV
                     AFDFANNGFTANLGLRLKLAILAFHFDYSLGNYGIFNAGVGINFR"
     gene            1088..2368
                     /locus_tag="PK049_10290"
     CDS             1088..2368
                     /locus_tag="PK049_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOK61988.1"
                     /translation="MPTIPLAERMRPRNLNDYVGQEHLVGSKGVIREMVTRGTITSFI
                     LWGPPGVGKTTLARVVANELNRPFYTLSAISSGVKDVRDVIEKAKSQKFFNSPSPILF
                     IDEIHRFSKSQQDSLLGAVEQGVFTLIGATTENPSFEVITPLLSRCQVYILKPLSNEE
                     LELLFVRATTTDPELKKFRFELRERDALYRFSGGDARKLYNIIELITSTQNTNNQVVI
                     TNKAVVDVLQQNLAIYDKNGEQHYDIISAFIKSVRGSDPNAAVYWLARMIEGGEDPEF
                     IARRLVILAAEDIGLANPNALLMATSCFQAVQMIGMPESRIILSEATIYLATSPKSNS
                     AYEAIGKAQGIVKETGDLPVPLHLRNAPTRLMKDIGYGIDYKYAHSFPGNFVEQEFLP
                     EKLSGTSIYKPQDNSREQEIAARIKALWKGKYNK"
     gene            2397..3203
                     /gene="kdsA"
                     /locus_tag="PK049_10295"
     CDS             2397..3203
                     /gene="kdsA"
                     /locus_tag="PK049_10295"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HOK61989.1"
                     /translation="MKGIKHIESGNFFLMAGPCVVESRDNVMRIAETVSSITNKLKIP
                     YIFKASYRKANRSRIDSFAGIGDEKALKILGEVNERFGLPIVTDIHSAPEAAMAAEYV
                     DILQIPAFLCRQTDLLIAAAQTGKFVNIKKGQFVSPDSMKFAAQKVVDSGNTNVILTD
                     RGTMFGYHDLIVDFRAIPIMRAFGFPVVVDVTHSLQQPNQTTGVTGGRPDMIETIARA
                     AIATGADGLFMETHFNPAEALSDGANMLNIKYLEGLLERLVAIRQVVKNF"
     gene            complement(3190..4587)
                     /locus_tag="PK049_10300"
     CDS             complement(3190..4587)
                     /locus_tag="PK049_10300"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOK61990.1"
                     /translation="MALVSTATTYFITHNYHKRKLSANAVNSSSHSVLLEQLREEIQK
                     LKIQCKELQIENDEQADTLAKAHLEIQRLEAAVQNAKIRAEEADILKSNFLANMSHEI
                     RTPMNGILGFAQLLKNEESQEKRDRYIDIIYHNGMMLVNLIDDIIDLSKIDAGQLTVN
                     PADCNLDNLMFEVYTFFNEIKFKQEKEHINIRLLNLNDDESNILYTDAQRLRQVLVNL
                     IGNALKFTERGSVEFGYLINENKSNIEFFVRDTGIGIPKDKQDIIFDRFRQVQEGSTR
                     KYGGTGIGLYISKHIIELLGGDIWFESEVGQGTTFHFSLPYHESKSVNDGSVIFKAKN
                     PEYKWEGITILIAEDIDTNYKYLSTVLADTKAKILWARNGEEAVSMVLNGSPVDIVLM
                     DIQMPVVDGYEATLRIRENYPDLPIIAQTAYALPHDNLKCFEVGCNDYVSKPINAHLL
                     KKKINDLLNVHVKSS"
     gene            complement(4800..5447)
                     /locus_tag="PK049_10305"
     CDS             complement(4800..5447)
                     /locus_tag="PK049_10305"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOK61991.1"
                     /translation="MKRTLFILALTSLSLFSNAQFFRMGLKGGISSSNVKFSKATITD
                     GANQYIVEQGDAKLGFHAGLFARVQIMGFFVQPELLFSRSQGEVMLKDVTLNDVYNEM
                     QKFNKFDIPVIVGWKFGPARVGLGPVATILISENDGLKDKLADLTNQTVKNNFRSATF
                     GYQVGVGLDILKTLTLDLKYEGNLSKLGTGITLGGTDYSFDQRNPQIIMSVGFFF"
     gene            complement(5504..6979)
                     /gene="cls"
                     /locus_tag="PK049_10310"
     CDS             complement(5504..6979)
                     /gene="cls"
                     /locus_tag="PK049_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HOK61992.1"
                     /translation="MVALNILTETFLNHLTTLLWAIYIITVLSTAAMIIHEKRDPAKT
                     STWVLLLITLPILGLLLYIVFGQNRRKEKLFSRKEIQDIEQIEYLSHKQVIRFSSKTD
                     IHHKLADHLSIITLLLNNSKALLTEKNEVEIFQTGNQAFDSIIESLYSATSSIHIEFY
                     IINDDKLGNRIKNILITKAKEGVKVRVLFDDVGSWSLPKRYVKELKDAGIEIYPFMEV
                     RFPLLTSRVNYRNHRKVVVVDGKVGYLGGMNIADRYIEGTRRLGQWYDTMLKIEGEAV
                     HSLQVIFLIDWYFVTGKIIRERNIYFPQPTVTSYHPLQIVTSGPDSDWASIMQAFFHA
                     ITRANKHIYISTPYFIPNESILTALKTAALSGIDVRIILPGKSDSTVVYWSSLSYVSE
                     LLEAGIKVYLYQNGFNHSKVMMIDGKLAMVGSANMDIRSFEDNFEVAAFIYDNQVTER
                     LEENFLSNLVQSQVITPQSWSKRPLKNSYKEALARLISPLF"
     gene            complement(7030..7857)
                     /locus_tag="PK049_10315"
     CDS             complement(7030..7857)
                     /locus_tag="PK049_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOK61993.1"
                     /translation="MTKALNKLSGKVVVITGASSGIGKALTYEFSKYNTKLVIGARNI
                     DELKAIASELSSVGVEVLPVKTDVTSESDCKNLVEQAISRYGQIDILINNAGVSMRAL
                     FKDVDLKVIRHLMDVNFWGTVYCTKYALPYLLETKGSVVGVSSIAGFHGLPGRCGYSA
                     SKFAMHGFLETLRIENLKNGLHVMIAAPGFTATNVRFSALTADGTPQGMSPRDEKNMM
                     TPEEVAKIIVNGIAKRRRNIIMGTEGKISVLAQRILPTVIDRLLYGYMAKEPNSPFK"
BASE COUNT         2464 a         1674 c         1645 g         2358 t
ORIGIN      
        1 cggaaattaa ttccaacccc ggcattaaaa ataccataat tgccaagtga gtagtcgaaa
       61 tggaaagcaa ggattgctag cttaagcctt aacccaagat tggcagtaaa accgttattg
      121 gcaaagtcga aagcaacggg gtcagtaaca gtcccaacgc cttcaacgtt ataggttccc
      181 ttaagtgaaa tatcggttgt tgaatggtta taccccaaac cgccataaac agttaaaact
      241 ggaatggact tagaaacaag taagcgagcc gtataacccg acgatttaaa attaagcttt
      301 tgcttttgtg ccttattgat gtcgaacgat gaagcgaact gagtgtaacc gaaaagaacc
      361 gacatgctaa agggcaatcg ttttacaaaa ggaataaatt ccttgaactc gttttttaca
      421 ccaacacccc acaaaccaaa gtttccaacg cttgaaatat ttatggttgg taagaaacga
      481 ccaactatct cggtgtgaaa gggtaatcct tttgctacct gaatcattgg ggcaggaaca
      541 aatttaaggt tggccccatc gggcatgttt aactcgctta gccctgttgc tttatcagtt
      601 aaaatggagt ttgaactttt acccgttact gttggtgagg tggaactaga accagatttt
      661 acctgccagt attccaggtt tagctttgaa acgtcaaacg ttttatcgga acttggtggt
      721 attgtaattg gaacagtaaa ggtgatgtcg aaccctaaaa ttccatgagg atttgcagaa
      781 ttgtaccacc cagaattcaa gcttgttccc aaactcttac caaaaggttc aaggtatgct
      841 ttactaagct tttcggcatc gttcaggctt cctttcagga atttagcaac atcttcctgc
      901 gcgtaaagcg ttcctgctat taggcttaga agcaggacga tagatttcct taacagcttc
      961 atatctattt ttttaaaggg tttaatttta aacaaataaa gcataaaaag agttcaatgt
     1021 caatatttat taaaaaatga accgtaaaaa aggcattatt acctaatttt gtggaaatat
     1081 ttttcccatg ccgacaatac ccttagctga aaggatgcga ccccgtaatc tgaacgatta
     1141 tgtagggcag gagcatcttg tgggtagcaa aggggttatt cgggaaatgg taactagagg
     1201 tactataacc tcgtttattc tatggggccc tccgggcgta ggaaaaacaa ctctggctag
     1261 ggttgtagca aacgagttaa atagaccctt ttacacccta agcgcaataa gttcaggtgt
     1321 aaaagatgtt agggatgtga ttgaaaaggc aaaatcacaa aaattcttca actcaccctc
     1381 gcccattctt tttattgatg agatacaccg attcagtaag tcgcaacagg actcactgct
     1441 tggtgcggtg gagcaaggtg ttttcacatt aattggcgct acaaccgaga acccctcatt
     1501 tgaggtgatt acaccattac tttcacgttg ccaggtttat atacttaaac cgctctccaa
     1561 tgaggaactt gaactacttt ttgttagagc aacaaccacc gatcctgagc tgaaaaaatt
     1621 taggtttgaa ctccgtgaaa gggatgctct ttacagattc tcaggaggcg atgcccgcaa
     1681 gctatataat atcattgaac ttataacatc aacacaaaat accaataacc aggttgtaat
     1741 aaccaataaa gccgtagttg atgtgctgca gcaaaacctg gcaatttacg ataagaacgg
     1801 agagcagcac tatgacatca tatcggcatt tattaaatcg gtaaggggta gcgatcccaa
     1861 tgctgctgtt tactggttgg cgcgtatgat tgaaggtggc gaggaccccg aattcattgc
     1921 acgcaggtta gttatattag ccgctgagga cataggcttg gctaacccaa atgcgttact
     1981 catggccacc agctgttttc aggcagttca gatgattggt atgccggagt cacgaattat
     2041 cctatccgag gcaaccattt atctggcaac aagccccaaa agcaattcag cctacgaggc
     2101 aattggcaaa gcacaaggga tagtaaaaga aaccggtgac ttgcccgtgc cactccatct
     2161 ccgcaatgca ccaaccaggt tgatgaagga tattggctat ggtattgact ataagtatgc
     2221 ccatagcttt ccaggaaatt ttgtggagca ggaattccta cccgaaaagc tttccggaac
     2281 ctcaatttat aaaccgcagg acaatagccg tgagcaggaa attgctgcaa ggataaaggc
     2341 actatggaaa ggtaagtata acaagtaatg ctaataatat gaacactatt gcaaagatta
     2401 aaggaattaa gcacattgaa tccggcaact tttttttgat ggctggacct tgtgtagttg
     2461 aaagccgcga taacgtaatg aggattgcag aaactgtatc gagcatcact aacaaactta
     2521 aaatccctta catttttaaa gcctcgtacc gcaaggcaaa ccgttcaaga atcgattcat
     2581 ttgctggtat tggcgatgaa aaggcactga aaattctagg cgaagtaaat gagcgatttg
     2641 gcttacccat tgttacagac attcatagtg ccccagaagc cgccatggcg gctgaatatg
     2701 ttgatattct tcagattccg gcattcctgt gcagacaaac agacctactg attgcagcag
     2761 cccaaacagg aaagtttgta aatatcaaaa aagggcaatt tgtatcaccc gactccatga
     2821 aatttgcagc gcaaaaagtt gttgattcag gcaataccaa cgttattcta actgacagag
     2881 gaacaatgtt tgggtatcat gatttaatag ttgattttag ggccatacca atcatgcgag
     2941 catttggatt tcctgttgtt gttgatgtta cccactcgct tcagcaacca aaccaaacca
     3001 ccggggttac gggtggcaga cccgatatga ttgaaaccat tgcccgtgct gccattgcaa
     3061 caggagccga tggactcttt atggaaaccc attttaaccc agccgaagca ttatcggatg
     3121 gggcaaacat gctaaatata aagtacctgg aaggattact tgagcgactt gttgcgatca
     3181 ggcaagtggt taagaacttt taacgtgaac gtttagcaaa tcgttaatct ttttctttag
     3241 gaggtgggcg ttaataggct ttgaaacata gtcgttacag cccacctcaa aacatttaag
     3301 attatcgtga ggtagagcgt aggcggtttg tgcaataatt ggtaaatcag gataattttc
     3361 tcttattctt aaagttgcct catacccatc aaccacaggc atttgaatgt ccattagaac
     3421 aatatcaact ggggaaccat tgagtaccat ggatactgct tcctcgccat ttcttgccca
     3481 aagaattttg gcttttgtat cggctagaac agtggaaagg tatttatagt tggtatcaat
     3541 atcctcagct atcaggatag ttatgccctc ccacttatat tcaggatttt tggctttgaa
     3601 aattacagaa ccatcgttaa cgctctttga ttcgtggtaa gggagggaaa agtggaatgt
     3661 agtcccctgc ccgacctccg actcaaacca gatatcgcca ccgagcaact caatgatatg
     3721 tttagaaatg tacaacccga taccagtacc gccgtattta cgggttgaac cctcctgtac
     3781 ctgacggaac ctgtcgaaaa taatatcctg cttatccttt ggaatgccaa ttcctgtatc
     3841 ccttacaaaa aattcaatat tgctcttatt ctcgttaatc aggtaaccaa actcaaccga
     3901 tcccctttca gtaaatttca gagcattacc aataaggtta accagaacct gacgtaaacg
     3961 ttgggcatcg gtgtatagga tatttgattc atcgtcgttc aggttgagta agcgaatatt
     4021 aatatgctct ttttcctgct tgaacttaat ctcgttgaaa aatgtgtata cctcaaacat
     4081 caggttatcg aggttacagt cggcaggatt aacggttagc tgtccagcat ctatcttgga
     4141 taaatcgata atatcatcta tgagattaac cagcatcatg ccgttatggt aaatgatatc
     4201 gatataccta tcgcgctttt cctgcgattc ctcattcttt aggagctggg caaatcctaa
     4261 tatgccattc attggggtgc gaatctcatg gctcatattg gccaggaagt ttgatttgag
     4321 gatatcggct tcctcggccc taatcttggc attttggact gctgcttcga gtcgttgtat
     4381 ctctagatga gcttttgcaa gtgtatcggc ctgttcatcg ttttcaattt gtagctcttt
     4441 acattgaatc tttaactttt gaatctcctc cctaagctgt tcaagcaaaa ccgagtggga
     4501 tgaactatta actgcatttg cagataattt tcttttatgg taattatgtg taatgaaata
     4561 tgtagtggct gtagatacta acgccacgat gattataatt ccgataccca taaattccaa
     4621 cataattatt ctatgcgaat tcaaatattc aactttttaa atacaataac aataattatt
     4681 tgcctaaaaa gcctaagaat tccttgaata gatttatagt ttaaataaac gacacgtttt
     4741 aaatgacaaa aggtttcctt agaatcattc taaagaaacc ttctgacatt aaataaagcc
     4801 taaaagaaga aaccaacgct catgatgatt tgtggattgc gttgatcgaa tgagtagtcg
     4861 gtaccaccca aagtgatgcc agttccaagt ttgctgaggt tgccttcgta tttaaggtcg
     4921 agggtaaggg ttttaagaat atcgagccct actcctacct ggtacccaaa agttgccgac
     4981 ctgaaattat ttttcactgt ttggttggtc aaatcggcta atttatcttt aagaccatcg
     5041 ttttcagaga ttaagattgt tgcaaccggt ccaagtccaa ccctagcagg accaaatttc
     5101 catccaacaa taactgggat atcgaacttg ttgaactttt gcatttcgtt gtaaacatcg
     5161 tttagggtaa catccttaag cataacctcg ccctgcgagc gggaaaaaag cagctcaggc
     5221 tgaacaaaga aacccataat ttgcacccgg gcaaaaagcc ctgcgtgaaa tccaagctta
     5281 gcatcgccct gctccacaat gtattggttt gcaccgtcgg taatggttgc cttactaaac
     5341 ttaacattgg atgagctaat gccgccctta agccccattc taaagaactg agcgttagag
     5401 aacagggaaa ggcttgtgag tgctaaaata aaaagtgttc ttttcatagt aatcggattt
     5461 aaaaaggttt aatgatctac tattatcaaa ttgcgtgcta gatttaaaag agtggactaa
     5521 ttaatcgtgc caatgcttcc ttgtagctat ttttcagcgg acgtttactc cagctttggg
     5581 gtgtaataac ctggctttgt accaggttgg ataagaaatt ctcctcaagc ctttcagtaa
     5641 cctgattatc gtaaatgaaa gccgccacct caaagttatc ctcaaagctg cggatatcca
     5701 tgttagccga ccccaccatg gccagcttac catcaatcat catcaccttg gagtggttaa
     5761 agccattttg gtaaaggtaa accttaatac ctgcctccag tagctcggaa acataagaga
     5821 gtgaactcca gtaaacaacg gtagaatcgg attttccggg taaaataatc ctaacatcaa
     5881 tcccacttag tgctgctgtt ttaagggctg ttaggatact ttcgttaggt ataaagtaag
     5941 gcgtagatat atagatatgc ttattggcac gtgttatggc atgaaaaaag gcttgcataa
     6001 tacttgccca gtcggaatcg ggaccgcttg ttacaatttg taagggatgg taactggtaa
     6061 cggttggctg aggaaagtaa atattacgtt ccctgatgat ttttccagta acaaagtacc
     6121 aatcaatcag aaagataacc tgaagtgaat gaactgcctc accctcgatt ttcaacatgg
     6181 tgtcgtacca ctggccaagc cttcgggtac cctcgatgta ccggtcagca atattcattc
     6241 cacctaaata accaactttt ccatcgacca ctactacttt tcggtggttt cggtagttta
     6301 ccctactggt aagcaagggg aatcgaacct ccatgaacgg ataaatctct atgccagcat
     6361 cttttagctc cttgacatac ctttttggta ggctccaact ccctacatca tcgaatagga
     6421 ctctaacctt aacgccctct ttagcctttg ttatcaggat atttttaatt cggttaccca
     6481 gcttatcgtc attaataata taaaactcga tatgaatact tgaagtggct gagtatagtg
     6541 attcaattat ggaatcgaag gcttgattac ctgtttggaa aatctcaacc tcattctttt
     6601 cagttagcag ggccttgctg ttgttgagca aaagggtgat aatacttaaa tgatcagcaa
     6661 gcttatggtg aatatcggtt ttagaggaaa agcggataac ctgcttatgg cttaaatact
     6721 caatttgttc aatatcctga atctctttac ggctaaacag cttttccttt ctacggtttt
     6781 ggccaaatac gatataaagt aataacccaa gtattggtaa agtgataaga agtaaaaccc
     6841 aagtggacgt tttagcggga tcgcgctttt catggataat cattgcggcc gtagaaagca
     6901 cggtaatgat ataaatcgcc catagtagcg tagttaggtg gtttaaaaac gtttcggtta
     6961 gtatgttcaa ggcaaccata cccaaatttt tctctaatta agaaaaattc gggaaattat
     7021 gcaaataacc tatttaaagg gcgaattggg ctcttttgcc atgtatccgt atagaagccg
     7081 atcgatcaca gttggcagaa tgcgttgggc cagtaccgat attttacctt cggtacccat
     7141 gataatattc ctacggcgtt tagcaattcc attaacaata atttttgcaa cctcctcggg
     7201 agtcatcatg tttttctcat cgcgaggcga cataccctga ggggtaccat cggcggttaa
     7261 agccgagaat ctgacgttag tagctgtaaa cccaggagcc gcaatcatta catgcaaacc
     7321 gttcttgaga ttttcgatac gtaaggtttc caggaaacca tgcattgcaa attttgatgc
     7381 tgaataccca catcggcctg gtaatccgtg gaatccagca atggaactca cacctactac
     7441 tgaacctttt gtctcaagca aatagggtaa tgcatacttt gtacagtaaa cagtacccca
     7501 aaaatttaca tccatgagat gccgaataac cttaaggtca acatccttaa aaagggcacg
     7561 catggaaacg cctgcgttat tgataagtat atcgatttgt ccgtacctgc ttatggcctg
     7621 ttcaactaag tttttacagt cactctcgct ggtcacatcg gtttttacag gcagcacctc
     7681 tactccaact gaggatagtt cagatgcaat agccttaagc tcatcgatgt tacgagcacc
     7741 aataaccagc ttggtgttat actttgaaaa ctcgtaggtt aaagcctttc caatgcccga
     7801 tgatgcacca gtaataacaa ctacttttcc cgatagttta tttaatgcct ttgtcatggt
     7861 attttaaaat attgttcagc taatctatta aataatggct tacatgagtt tgaattactg
     7921 ttaaagtgta acatttaatc tttttaacat aattcatcaa tctgataatc aatgtctaga
     7981 acaatattta catttgaaat gaaagaaaaa tttgtatatt ttagagaaag agtctatctt
     8041 tgcaacgcaa aaaacaaaag tagttctcaa caaaataggt agattcagta gctcagcagg
     8101 tagagcacat cccttttaag gatggggtcc tgggttcgaa t
//
LOCUS       DAOSAL010000092         8092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_5777_length_8092_cov_49.966903, whole genome shotgun sequence.
ACCESSION   DAOSAL010000092 DAOSAL010000000
VERSION     DAOSAL010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 8092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8092
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5777_length_8092_cov_49.966903"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..2615
                     /locus_tag="PK049_10320"
     CDS             69..2615
                     /locus_tag="PK049_10320"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOK61994.1"
                     /translation="MRAKVVSITLLALTFAFFTVNASERDSLLNLLSASKGKDKVEIL
                     IKLTEATSGTDEAIKYADEAIVAARKINDELLAKAYLNKGYALSENYQDNDAIEYYRK
                     ALELAQKNGFKSSILEAYKGISTSYFYIDSFGESQRYASLLLQQALELDSLRYQAYAY
                     FRLGKILQEQGKNDSALHYIRRSLGLREKLGDWKEIALTYNGLGRIYFDLAQYDSAIW
                     CYSKEVELKEQMGDQPAYLAIAYLNIGRTHIAISNFQLALEQYQKALRTFESINHQEG
                     IATCNSGLGMIYENLSQSILATDENEANYLKALGYHKTALELFHNLQKKKEEGQTLQN
                     IGNVFSRLATNRYVARFGEMWEDSLYKIKQTEILASFDSARHYYNKALSIFEELHEEG
                     EIAKVNTNIGSIYSWARDWEKANTYINRALQLARKNNLLYETTAALYAKGESLFRQGR
                     SEEAEIAFIECSRLSERLGLKETLRYSYERLSKLYEHRNDLVRAYAYFKQAVKIKDEI
                     FSEKSQRVMAEMQTKYETEKKEQQLVLMKNKDELQKSIIQRQKLMIIGAIAGSFLILM
                     VALLMFKMFRDKQRANIILEEKNALITRQKQEITDSIKYASRIQTAVLPSSALISDVL
                     PDHFVLFLPRDIVSGDFYWMTRKNEKIVLVAADCTGHGVPGAFMSMLGVSFLYEIVNK
                     ESILQPAEILNNLRNHIKTTLSQTGKIEEQKDGMDISLCVIDKQNQKLEWAGAYNPLY
                     QISNGELIEYKADKMPVAVHINDFNLFTNHEIEYQPGDAFYMFSDGYADQFGGTDGRK
                     FMLKRLKETLLSIWEKPMAEQKEILHKIHMDWRGEQNDQVDDILIIGFRV"
     gene            complement(2605..3873)
                     /locus_tag="PK049_10325"
     CDS             complement(2605..3873)
                     /locus_tag="PK049_10325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOK61995.1"
                     /translation="MWVKYFTKKGFSPKIDVNPSVNLGICVVIPCYNEPSLIESLGSI
                     AGCNLPACDVEVIVVVNHPEGVHYDVLENSNKAVKDIDEANRLWGCERLRFHAVKAFN
                     LPRKQAGVGYARQIGMDEAAFRLLTVGTKQGIIACFDADSQCMPNYLAELYELWMQYP
                     KTHACSVRFEHPLSGDEFPAEIYKGITAYELHLRYYNQASRFIGFPFAYHTVGSSMAC
                     SAEAYVKFGGMNRHQAGEDFYFLQKIIPHGHFRELNTTCVYPSPRPSNRVPFGTGRAM
                     SKLVYSREPISTYHLESFLNLEPLFQNVTTLYNYSGNQIERFIELLAYPLSDFLFQVD
                     AARAIENVKSNASSAKTFKKRFFLWFDAFMLLKYMNFAAERYYGRQPVEEQSKSLLAC
                     MGYKPLQTLRTRDLLLFYREMESVEWSAKL"
     gene            complement(3959..4564)
                     /locus_tag="PK049_10330"
     CDS             complement(3959..4564)
                     /locus_tag="PK049_10330"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOK61996.1"
                     /translation="MKKTNLIINILFGVAIVVLFVLHFTMGSKRGDVKGTEGNAVLTE
                     QFTAAWVNMDTLMNSYDMYFDMKKELEESGRKKEADLNAKSRSFEKEAYDFQDKVQKG
                     LLTRSEAQQLQTNLAAKEQQLYQLRDEMRMQLAEEEQVKLRLIHNSIIEYLKEYNADK
                     GYHLILSNTFGGPLLYGNPANDITKEVLEGLNKKYAADKKK"
     gene            complement(4708..5091)
                     /locus_tag="PK049_10335"
     CDS             complement(4708..5091)
                     /locus_tag="PK049_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES family protein"
                     /protein_id="HOK61997.1"
                     /translation="MSLSFDPKDFDSIIVVGDRVLIKPKNPDERTKAGLLLPPGVQEK
                     QKAHSGYVVKVGPGYPIPMFTEADEPWKKSRNDAKYFPLQAQVGDLAVYLHENAIEVE
                     FNSEKYVVVPFSSILLLFRDEGLLE"
     gene            5257..5673
                     /locus_tag="PK049_10340"
     CDS             5257..5673
                     /locus_tag="PK049_10340"
                     /inference="COORDINATES: protein motif:HMM:NF023390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HOK61998.1"
                     /translation="MGSIEIFNSHYAVGIYNPEKKCVEVTWHGNQTIEEYKALFEALL
                     EFQRNSGLEIKGYLSDIRDQGVVNPDSRKWFEQVALPQAINQGLKWAAVVFDGNTFKR
                     YYLNLILQVANIYGLPLKFFNSKEDAYKWAETPNLK"
     gene            6006..>8092
                     /locus_tag="PK049_10345"
     CDS             6006..>8092
                     /locus_tag="PK049_10345"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOK61999.1"
                     /translation="MTQVPANIHEVPFVRLAIPLIAGILIQYYLNPLNANLTIGLILI
                     LLLLLIYDRIFIKSWEKRWIFGLIINLLLALFGSLLVTINRPETTFKSNSNYSMLVRL
                     TEPPVTGSNSIKVKAEIIDIKTYANLESPKPVLLYFLLSDSASHGLNYGDILALSGNL
                     KEFSEPPNPFQFNMKRYMYLLGIVYYIRVNQGCWEKVGSNPSKIFKLAYMAQSYVIET
                     LKGYGIKGQELAVISALMLGYKSLLDDEIRQVYSSVGAMHILAVSGLHVGLIFSVVVI
                     IITLLPKRRYWLAIRLGIALTVLWGYALVTGLSPSVCRATIMFSLFAIGQTAGLKTNS
                     YNTLAAAAFLLLVSNPFMLFNVGFQLSFAAVLSIVFFHPTIYSVLKPKSKLGDYIWSL
                     LSVSAAAQILTLPLTLYYFGQFPVVFLVTNLIAIPLATIALYLSLITIAFSLIEQLGF
                     LFAKILGFVTWLLNSGLKLVDTIPFSSISSIYFTSWQAILLLLSILMLSVYLVNRYRP
                     FLQYSLISLIGVLAISALHLVRVKNQAELVVFPVPKSSVICVNSNGNALLVLYDSIDT
                     KFSTDGYIRNRALQKALEINLNFYQEQEHSNQLKIIKKSGLALLVAGNKTIALAYNDS
                     ARFLKSIKPLNVDILIANRFYSNSLLNIITPQKVIIDPSIKSAYANRLSSTLIKNNIA
                     FYNTSVSGFFEYNL"
BASE COUNT         2388 a         1756 c         1658 g         2290 t
ORIGIN      
        1 atttttatca aattcctttg cataatgctg taatctttag taatttgctc acgaatcatt
       61 gtgttaccat gagggcaaaa gtcgtatcaa tcaccctatt agcgctaacc tttgcatttt
      121 ttacggtaaa tgcaagtgag cgcgatagct tacttaacct tctttcggcc tcaaagggta
      181 aggataaggt tgaaatcctt ataaagctta ccgaggcaac ctccggcacc gacgaggcca
      241 ttaagtatgc cgatgaggcc attgtggctg cgcgcaaaat aaacgatgag ctgcttgcaa
      301 aagcttacct gaataagggc tacgccctat cggaaaacta ccaggataat gatgccattg
      361 agtactaccg caaagcgctt gagctggcgc aaaagaatgg ctttaaaagt tcgattctgg
      421 aggcctataa gggaatttct acatcgtact tctatatcga ttcgtttggg gagtcgcaaa
      481 ggtatgccag cctgttgctt caacaagctc ttgagcttga tagccttagg taccaggcat
      541 acgcatactt caggctaggg aaaatacttc aggaacaggg caaaaacgat tcagccctac
      601 attacattcg tcgctccctg gggttaaggg aaaaacttgg cgactggaag gagatagccc
      661 ttacctacaa tgggcttggg cgtatttact ttgaccttgc ccagtacgat agcgcaatat
      721 ggtgctacag caaggaggta gagcttaagg agcaaatggg cgaccaacct gcctaccttg
      781 ccattgccta cctcaacata gggagaactc acattgccat aagtaacttt cagcttgccc
      841 ttgagcagta ccaaaaggca ctccgtactt ttgaaagcat aaaccaccag gaaggaatag
      901 ccacctgtaa ctcggggttg ggtatgattt acgagaatct atcgcaaagc attcttgcca
      961 ccgacgagaa cgaggccaac taccttaaag cactgggcta ccataaaaca gctcttgaac
     1021 tattccataa tctgcaaaag aaaaaggagg agggccaaac ccttcagaat attggcaatg
     1081 ttttttcgcg attagccact aaccgttatg ttgcccgctt tggcgaaatg tgggaagact
     1141 ccctttacaa gattaagcaa accgaaattc ttgcctcgtt cgatagcgca aggcattact
     1201 acaataaagc ccttagcatt tttgaagagc tgcatgagga aggcgagata gccaaggtaa
     1261 acaccaatat tggcagcatt tacagctggg ctcgcgactg ggaaaaggca aacacctata
     1321 tcaaccgtgc tctgcaactg gcacgaaaaa ataatctgct ttacgaaacc acagcagccc
     1381 tttacgcaaa gggcgagagc ctattccgtc agggacgatc cgaagaggct gaaatcgctt
     1441 tcatcgagtg ttccaggctc tccgaaaggc ttggcctaaa ggaaacttta cgctacagct
     1501 acgaacgcct atcgaagcta tatgagcatc gtaacgattt ggttcgggca tacgcatact
     1561 ttaagcaagc ggtcaaaata aaggatgaaa ttttcagcga aaaaagccag cgcgtaatgg
     1621 ccgagatgca aaccaagtac gaaaccgaaa agaaggagca acagcttgtg ctgatgaaga
     1681 ataaggatga actccaaaaa tcgattatcc agcgtcaaaa gttaatgatt ataggtgcca
     1741 ttgccggttc gttccttata ctcatggttg ccctactcat gttcaagatg ttccgcgata
     1801 agcaacgcgc caatattatt ctggaagaaa aaaatgcgct gattacccgc cagaaacagg
     1861 aaattaccga tagcattaag tatgccagcc gcattcaaac agcagttttg ccttcatcgg
     1921 cacttatttc cgatgtgctc cccgaccact ttgtgctttt cctgccccgc gatatagtta
     1981 gcggtgactt ttactggatg acccgtaaga acgagaaaat tgtactggtt gctgccgact
     2041 gtaccggaca tggtgtgccc ggtgcgttca tgagcatgct gggtgtgtcg ttcctttacg
     2101 agattgtaaa taaggaaagc atacttcaac cagccgagat acttaacaac ctccgtaacc
     2161 acattaaaac aaccctaagc caaaccggta agatagagga gcaaaaggac ggaatggaca
     2221 tttcgctttg cgtaatcgat aagcaaaacc aaaaactgga gtgggctggc gcctacaatc
     2281 cgctttacca aataagtaat ggcgaactta ttgagtataa ggccgataaa atgcctgttg
     2341 ctgttcacat caacgacttc aacttgttca ccaatcacga aattgaatac cagcccggcg
     2401 acgcctttta catgttctcc gatggatacg ccgaccagtt tggcggtacc gatggaagaa
     2461 aattcatgct aaaacgcctc aaggaaacct tacttagcat ctgggaaaag ccaatggctg
     2521 aacaaaagga aatacttcat aagattcaca tggattggcg cggtgagcaa aacgaccagg
     2581 ttgacgatat actgattatt gggttcagag tttagctgac cactccacac tctccatctc
     2641 cctatagaac aggagcaaat cccttgtcct gagcgtttgt aatggtttat agcccatgca
     2701 ggccaaaagg ctttttgact gctcctcaac tggttgtctg ccgtagtacc tttccgcagc
     2761 aaagttcata tacttcaata gcataaaggc atcgaaccaa aggaagaatc gttttttaaa
     2821 cgttttagcc gatgatgcat tggattttac attttcaatg gctctggcag catcaacctg
     2881 aaaaagaaaa tcactcaaag gataagccag caattcaata aacctttcga tttgattacc
     2941 actgtaattg tatagcgtag taacattctg aaacagcggt tcaaggttaa gaaacgattc
     3001 aaggtgatag gtagatattg gctcgcggct gtaaaccagc ttactcattg ccctgcccgt
     3061 tccaaaggga accctgttcg agggtcttgg cgaagggtaa acgcaggtgg tgttaagttc
     3121 ccggaagtgt ccatggggaa taatcttttg aagaaagtaa aaatcctccc ctgcctgatg
     3181 gcgattcatt ccgccaaatt ttacgtatgc ttcagcactg caagccattg atgaacctac
     3241 tgtatggtag gcaaacggaa aaccaataaa acgcgaagcc tgattatagt accggaggtg
     3301 tagctcatag gcggtaatgc ctttgtatat ctccgcagga aactcatcgc ctgaaagagg
     3361 atgttcaaag cgaactgaac atgcatgggt ttttgggtat tgcatccaca gctcataaag
     3421 ttccgcaagg tagtttggca tgcactgtga gtcggcatca aaacaggcta tgattccctg
     3481 tttcgtacca acggtaagca gcctaaaagc cgcctcgtcc ataccaatct gccgggcata
     3541 tccaacacca gcctgtttgc gcggtaaatt gaatgcctta acggcatgaa atcgcaaccg
     3601 ctcgcaaccc cataatcggt tcgcctcatc aatgtctttg actgctttat tggaattctc
     3661 caacacatca taatgtaccc cttcgggatg atttactaca actattacct caacatcgca
     3721 ggcgggtaaa ttgcaaccgg caatcgaccc caacgattct attaagctag gttcattgta
     3781 acagggaata acaacacaaa tcccaaggtt aaccgaaggg ttaacatcaa ttttaggcga
     3841 aaaacccttt ttggtgaagt attttaccca catggcgttg caaaaatagc gaaacccttt
     3901 ctcatgaaaa acattcgaaa gggtttcggg ttggtttagg tttagtatag gtgggtattt
     3961 atttcttttt atcagcagcg tactttttgt ttaagccctc aagtacttcc ttggtaatat
     4021 cgttagcggg attcccgtat aatagcgggc caccaaaggt gttactgaga attaaatggt
     4081 aacccttatc ggcgttgtac tccttaaggt actcaatgat actgttgtga atcaaccgaa
     4141 gcttaacctg ttcttcttcg gcaagctgca tgcgcatctc atcgcgaagc tggtatagct
     4201 gctgctcttt agcggcaagg ttagtttgaa gctgctgagc ctcggagcgg gtcaataaac
     4261 ccttttgaac cttatcctga aaatcgtagg cttccttctc aaagcttcgc gattttgcgt
     4321 taaggtctgc ctctttcttt ctaccgcttt cttcaagttc ttttttcatg tcgaagtaca
     4381 tatcgtagct attcatgagg gtgtccatgt taacccatgc ggcagtaaat tgttccgtta
     4441 atacggcatt cccttcggtt cctttcacat cacctctttt gctacccata gtaaaatgga
     4501 gaacaaaaag aacaacaatg gcaacgccaa acagaatgtt gattattagg tttgttttct
     4561 tcataattaa ttgatattac tgattagtat atttcaagtt attcaaaacg cacattaaat
     4621 caaacatcca caaacatact cacgcaaata taaatgaaaa tgaaacataa caaaatattt
     4681 cagccagttt tttcccattt aaaacgatta ttccaataat ccttcgtcgc ggaaaagtaa
     4741 taaaattgat gagaaaggaa caaccacata cttctcgctg ttgaactcaa cctcaatagc
     4801 attttcgtgg aggtaaacgg ccaggtcgcc cacctgcgct tgtaggggaa agtacttagc
     4861 atcgttccta ctctttttcc aaggctcatc ggcctcagta aacatgggga ttgggtatcc
     4921 gggtccaacc tttaccacat agccgctgtg ggccttttgc ttttcctgaa ctcctggagg
     4981 tagcaaaagc ccagccttag tgcgttcatc ggggtttttg ggcttaatca gaaccctatc
     5041 gcctactaca ataatactgt caaaatcttt gggatcgaat gaaagtgaca tattactcat
     5101 tgtttatcac agcaaaatta tcgttttgtt tttaaatgta cgttttttgt acttagattt
     5161 agttattcta tccacaaaat tatgatgtgt gttattagct tattgacttg cacactgtat
     5221 attactatct tgcaactgac aaccattaca acatttatgg gttctataga aattttcaac
     5281 agccattatg cggtagggat atacaacccc gaaaaaaaat gcgttgaagt tacatggcac
     5341 gggaatcaaa ccattgagga gtacaaagcg ctttttgaag cacttttgga attccagcgt
     5401 aactccggat tagagataaa agggtatctt tccgacatac gagaccaggg tgtagtaaac
     5461 cccgacagcc gaaaatggtt tgagcaggta gcgctaccac aggctattaa ccagggctta
     5521 aaatgggctg cagttgtttt cgatgggaat acattcaagc gttactacct gaaccttatc
     5581 cttcaggtag caaacattta tggattgccc ctaaaatttt tcaattcaaa ggaggatgcc
     5641 tataaatggg ctgaaacacc caatctcaaa tagtttacaa ctaatcatat tgacccttcc
     5701 ctttaaccca agtgtcatga tttaacatat tcaacctgac taatttttag aagtttgagt
     5761 cggcatgagt cggaatagac agtatatgat ggagttagag aaagttagag gatttcgtca
     5821 attgattatt tcaattgacg aatcgactaa gaaatataat cattcctaga ctccggaatg
     5881 aaagattatg aaaagtcagg cttgcctcga taaatcaata gaaacaactt gtttaaattt
     5941 ttttgcgttt ctattgaata atctcaatta aaaaagttgt aacttgaatt gttgttacaa
     6001 ccaatatgac ccaagttccc gctaatatcc atgaggttcc tttcgttcga ctggctatac
     6061 cgttaatagc tggtatcctt attcagtatt acctaaatcc gctcaatgcc aatttgacaa
     6121 taggcctaat attgatttta ctattgctgt tgatttatga ccgaattttt atcaaatctt
     6181 gggaaaagcg ctggattttt gggttgatta taaatcttct actcgcactt tttggaagtt
     6241 tgctggtaac cataaatagg cctgaaacaa cctttaaatc aaattcaaac tatagcatgc
     6301 tagtaaggct tactgagcca cctgttacag gaagcaattc aattaaggtt aaagccgaaa
     6361 ttatcgatat taaaacatat gctaatcttg aatcgcccaa accggtgttg ctatactttt
     6421 tactgtcgga tagtgccagc catggattaa attatggcga tatattagca ctatcgggaa
     6481 atctaaagga attttcggaa ccacccaacc ccttccagtt caacatgaaa aggtacatgt
     6541 accttttagg aattgtttac tatatcaggg ttaaccaggg ttgctgggaa aaagttggaa
     6601 gtaatccaag taaaatcttc aagttagcat acatggccca aagctatgta attgaaaccc
     6661 tgaaaggata cggaataaaa ggccaagaac tggcagtaat atcagcgtta atgctggggt
     6721 ataaatcgtt gctcgacgat gaaatccgac aggtttactc ttcggtaggg gccatgcata
     6781 ttcttgccgt gtcgggtttg catgtggggc tgatttttag cgtggtggta ataatcatca
     6841 cccttttacc caagcgccgt tactggttgg caatacgatt gggtatagct cttacagtac
     6901 tatggggtta cgccctggtt acagggcttt caccatcggt atgtagggct accattatgt
     6961 tttctctctt tgccataggg caaacagcag gcttaaaaac caatagctac aacaccctgg
     7021 cggcagcggc ttttttgctt ttggttagca atccgttcat gctattcaac gtgggctttc
     7081 aactctcgtt tgccgctgtt ctgtctattg tattttttca tcctaccatt tacagtgttc
     7141 ttaaacctaa aagtaagctt ggcgattata tttggtcgct cttgtcggtt tcggcggcag
     7201 cgcaaatcct taccctaccc ctaacgcttt actactttgg ccaattcccg gtagttttcc
     7261 tggttacaaa tcttattgca attccgctgg caacaatagc tctttaccta tctttaatta
     7321 cgatagcttt ttcgttaata gagcaattgg gctttctgtt tgctaaaatt cttgggtttg
     7381 tcacgtggct tttaaattcg gggcttaagc tagttgacac cattcccttt tccagcataa
     7441 gttcaattta ctttaccagc tggcaagcaa tactgctttt gctttcaatc ctaatgttat
     7501 cggtatatct agtaaatcga tacagacctt ttttgcaata ttcattaatt tcgcttatag
     7561 gagtacttgc cattagtgca ttacatttgg tgagggttaa aaatcaggcg gagctggttg
     7621 tatttccggt acccaaaagt tcagtaatat gtgtaaacag caacggaaac gctctattgg
     7681 ttctttacga ttcaatcgat accaagtttt caacggatgg gtacatcagg aatcgggcgc
     7741 tacaaaaagc ccttgaaata aacctaaact tttaccaaga gcaagagcat tcaaaccaac
     7801 taaaaattat caaaaaatcg ggtttggcat tacttgttgc tggaaacaaa accattgcat
     7861 tagcctataa cgatagcgca agattcctta aaagcataaa gcccttaaat gttgacatac
     7921 ttattgctaa tcgcttttat agcaatagtt tattaaacat cataacccca caaaaggtga
     7981 ttattgaccc aagcattaaa tcagcatacg ctaatcggct ttcatcaaca ctgattaaga
     8041 acaatatcgc tttttacaat acaagcgttt cgggcttttt tgaatataac ct
//
LOCUS       DAOSAL010000093         8026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_5836_length_8026_cov_56.376741, whole genome shotgun sequence.
ACCESSION   DAOSAL010000093 DAOSAL010000000
VERSION     DAOSAL010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 8026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8026
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5836_length_8026_cov_56.376741"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            208..537
                     /locus_tag="PK049_10350"
     CDS             208..537
                     /locus_tag="PK049_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOK62000.1"
                     /translation="MNKSEVFDEKLQELARFARAISHPARLAILQYLAETRTCISGDI
                     SQNLPLSRTTVSQHLKELKELGLIHGEIDGLRINYCLCSESIHKYLDEFDSFFGKVRS
                     ASVNCEI"
     gene            556..843
                     /locus_tag="PK049_10355"
     CDS             556..843
                     /locus_tag="PK049_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK62001.1"
                     /translation="MKLSVKAKEELLKVLETIDENSKGIRIYSTQGCCGPSVQMDVAS
                     APQPGETATSIDGVDFYIEDSLAETLAEVTLDHNGFGFLMNGLKKSGSCCG"
     gene            856..1272
                     /locus_tag="PK049_10360"
     CDS             856..1272
                     /locus_tag="PK049_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOK62002.1"
                     /translation="MEANPFSGNGFMSGGFLNLTPRDAYYEAKTGKAIIVDVREENLT
                     GYKRFDVPKVIYLPLSELKQNLGLLPTDFPLVIADSTGLRSHEAMEILIARGFTLIAN
                     LAGGIVEWERDGLPLVINNKERLDGSCMCQLRPRFK"
     gene            1286..1699
                     /locus_tag="PK049_10365"
     CDS             1286..1699
                     /locus_tag="PK049_10365"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011107123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOK62003.1"
                     /translation="MTRILILCTGNSCRSQMAEGFLKSFDKQLTVESAGTEPAAQVNP
                     NAVKVMAEVGIDISKNKPKLVDQFIGQEWDYVITVCDNAKETCPAFLGKVKHRLHIGF
                     EDPAEAKGTPEEVMAVFRKVRDEIKDRFSEFTKTI"
     gene            1703..2761
                     /gene="arsB"
                     /locus_tag="PK049_10370"
     CDS             1703..2761
                     /gene="arsB"
                     /locus_tag="PK049_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HOK62004.1"
                     /translation="MKKRLKFLDRYLTLWIFLAMAIGVLSGHFFPGIADFWDSLKPSP
                     ESTTNIPIAIGLILMMYPPLAKVKYEELGDVFRNYRILSLSLVQNWLIGPVLMFALAI
                     TFYKLFPSETNLQYMYGIIMIGLARCIAMVIVWNDLAKGDTEYAAGLVAFNSIFQVLF
                     YSVYAYVFIAILPGWFGIPTNNSVEQITIGQIAESVFIYLGIPFIAGFITRFTLVRLK
                     GDNWFNTKFIPKISPLTLIALLFTIFVMFSLKGEYIVKIPMDVVLIAIPLLAYFVIMF
                     LVSFFMSRKAGATYEQTTTLSFTAASNNFELAIAVAIAVFGISSGEAFAAVIGPLVEV
                     PVMIGLVNVAFYFKKRFF"
     gene            2819..3286
                     /locus_tag="PK049_10375"
     CDS             2819..3286
                     /locus_tag="PK049_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HOK62005.1"
                     /translation="MEIERKFLVDLPKWEKLDKPNPIRIVQGYLSTNPEQTIRVRVWA
                     DKAFMTIKGKVQGISREEIEFEIPTGKANELLTKFCKAVIKKNRYLIVHNSKTWEVDV
                     FEGENAGLVLAEIELGSEDEKFTVPHWITIEVTDDYRYYNSYLSENPYSSWKK"
     gene            3293..3763
                     /locus_tag="PK049_10380"
     CDS             3293..3763
                     /locus_tag="PK049_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK62006.1"
                     /translation="MIIIEIPGRKTLRIEHLVLDFNGTLAIDGKLIEGVRERLQQLST
                     QLMVHVITADTFGTATHELRGIDCKVKVLEKGRQDKQKKIYINELGKNKCATIGNGYN
                     DWQMLKHAALSIATIQAEGASTLSLKQASIVCYNVNHALDLLLNPNRLVATLRR"
     gene            3900..7697
                     /locus_tag="PK049_10385"
     CDS             3900..7697
                     /locus_tag="PK049_10385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOK62007.1"
                     /translation="MASKVALLIINQILPVDKFETILEAKGYSCLTLDRISSDIELPI
                     FNIAIVGLDNEKTYLKLKEKTDDYDGVILFFSGKHYNEIASQIAKLPESLHIEPNFSG
                     KELELAIQLQLLKKQIQKAESFASSNFDTNHVRLKKIITEEQAWRIIFEQSPNGVLLS
                     DDEGNIIYTNSAATDILGYTRDEFLKMRFHDLAPKELAEQVDTNIKKILRGESLITEV
                     YNIRKDGSKRYVQLHETRVIFPNGKYGIMVISTDISRAKQAEEALKESMEMYRILVEH
                     SNDGIVFVKEGIVIYGNPRIIEILNAKPEEFLNQPITKFIHPSQKEFLLQRYKNRLAG
                     KSEPTIYETILVSSQNEEINVEFNVNLVDQAGEKIDIVFIRDITTRKQAEKAIKESEE
                     SYKGLFNNSSEGILIIDSSGVILDANRSGQILFGYSKDELLGKTIHEITDNTQTSLEE
                     VTRSVRLAYQGHTQKMEFWGIRKNNDLVPIDMVFNRGDYFGLSVIFAMCHDITDRKNA
                     EAVLRESEEKYRTLTEQIPVGLYRILSNGQIVYCNPAFAEIFGFSSSQAATGSNIKSF
                     LRDFDKWLVDCSTAKLPIEVEMLRADGSTIWVQNILQPNVTENPDATYFDGMLTDVTD
                     RKVAIDELTESEAKFRAMLLAIPDHLFRLNREGILIDFAPTDIAFYPEISNLHLGKSI
                     AEIFPTSIYEKYSEALNYASITGKLQRFEYSFRQENSIFYYEARIVPAGEKIFLVLLR
                     DITQQKKFEEEIRILAQTIMNANDSISITDLDGNFIYVNPAFTKVYGYSQTEILGKNI
                     NLIRPIGFDSDLSKEIHSATQKGGWKGEILNVRKDGTIFPIFLSTSPVYNEFNEPIAS
                     VGIANDITERKLIEQELIRAKEQAEESDRLKTAFLSNMSHEIRSPMNAVLGFIQLLKA
                     EESITETGKQYIELIENSGNQLLALIEDIIDISKIQSNQIRINKTSFDFNELLRELYT
                     IYSNQLIRKADQKTLLFKPEMPQESPFTIYSDPVRVRQILNNLLSNAIKFTPAGHIRF
                     GYTLMIDEDMPYIQCYVQDTGIGISPEKQALIFERFRQADDTYTRIYGGSGLGLAISK
                     GLVDLLGGHIWVESTEGQGSTFYFTIPFQEGTESGKSLKKEEQKTLSSELLKGKKILV
                     VEDISEIRLYFERLLEPTGAKTLFAHNAREARVLFKKHKDISVVLLDIRLPDADGYTI
                     AAEFKRLRPDLPIIAQTAYALQSEFNKSIESGCDDYVTKPIDSPLLIQKILSLIEKKS
                     DNH"
     gene            complement(7694..>8026)
                     /locus_tag="PK049_10390"
     CDS             complement(7694..>8026)
                     /locus_tag="PK049_10390"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase"
                     /protein_id="HOK62008.1"
                     /translation="ERQVRIEGKIHKATDAESDRYFKTRPEASKIGAWASPQSTVIPN
                     RKYLERLMADFEEEFKGKTVKRPPNWGGYTLVPTLFEFWQGRPNRLHDRIQYTLINNQ
                     WKIERLAP"
BASE COUNT         2493 a         1700 c         1710 g         2123 t
ORIGIN      
        1 tttacatata ccctaaacgg aaaacttacc gattgtaaag tatgtgggca gagtccgcca
       61 cttatggatt aaaaaaatcg cagattttga gcgagggaag aactgtatat atactaatca
      121 atatcttaaa atttgtttga aacattttac ctgaattgtt gtttaagcca aaaatatgtc
      181 gtatgtttac gacataaata caatggtatg aataagtcag aagtttttga cgagaagctg
      241 caggaattgg caaggtttgc cagggccatt tcgcatccag ctcggttggc aatactccag
      301 taccttgccg aaaccaggac atgcatatcg ggcgatattt cgcagaacct gccgcttagc
      361 cgcactaccg tttcgcagca tttaaaggag ctaaaggaac tagggctcat acatggcgaa
      421 attgatggac tacggataaa ctactgcctc tgctcggaga gcatacataa atacctcgat
      481 gagtttgact cctttttcgg taaagtaagg agcgcatcag taaattgtga gatttaatcc
      541 ataaataatt taactatgaa actatcagta aaagcaaagg aagaactatt aaaggtttta
      601 gaaaccattg atgaaaactc aaagggaatc cgtatataca gcacgcaggg atgctgtggc
      661 ccttcagtgc aaatggatgt tgcaagcgca ccacagcccg gcgaaactgc cactagcatc
      721 gatggtgtag atttctacat tgaagattca ttagctgaaa cccttgccga ggttaccctc
      781 gaccataacg ggtttggatt cctgatgaat ggcctgaaaa aaagcggtag ttgctgcggc
      841 taaaaacaga atgccatgga agccaatccc ttttccggta atggttttat gagcggaggt
      901 tttctcaacc taaccccacg cgatgcttac tacgaggcta aaacggggaa agccataatt
      961 gttgatgtta gggaagagaa ccttacaggc tacaaacgct tcgacgtacc caaggttatc
     1021 tacttacctt taagtgagct aaagcagaac ctagggttac ttcccactga tttcccgcta
     1081 gtaattgccg actcaacagg gttgcgaagc cacgaagcaa tggaaatact tattgccaga
     1141 ggtttcacat taattgctaa ccttgccggc ggtattgttg agtgggaacg cgatgggctt
     1201 ccattagtaa tcaacaacaa ggaacgcctc gatggctcct gtatgtgtca gctaaggcca
     1261 aggtttaagt aaactttata tcaaaatgac aagaatactg attttatgca caggcaacag
     1321 ctgtcgaagc cagatggctg aaggatttct gaaatcgttc gataaacagc taaccgttga
     1381 atcggccgga acagaaccgg ccgcacaggt aaacccaaac gccgtaaagg tgatggccga
     1441 ggtgggcatt gatattagca aaaacaaacc caaactggtt gaccagttta ttggccagga
     1501 atgggattac gtgattacgg tttgcgataa tgccaaggaa acctgtccgg cattcctggg
     1561 taaagtaaag cataggcttc acattggttt tgaggatccg gccgaagcaa aaggaacacc
     1621 tgaggaggta atggcggtat tccgaaaagt tagagacgag attaaggata ggtttagtga
     1681 gtttactaaa acaatttgat tcatgaaaaa acgtctgaag tttctggaca ggtacttaac
     1741 cctgtggatt tttttggcaa tggccatagg tgtactaagt ggccactttt tcccgggaat
     1801 agctgatttt tgggattcat tgaaaccttc gcccgaaagc acaaccaata ttcccattgc
     1861 aattggacta atactaatga tgtatccgcc ccttgctaaa gtaaagtatg aggagcttgg
     1921 cgatgttttc cgaaattaca gaattctttc gctttcgctt gttcagaatt ggctcatagg
     1981 gccagtactc atgtttgcac tagcaataac tttttacaag ctgttcccca gcgaaaccaa
     2041 cctgcaatac atgtacggca tcataatgat agggcttgca cgttgcattg ctatggttat
     2101 tgtttggaac gatttggcca aaggcgatac tgagtatgcg gctggcttgg ttgcttttaa
     2161 ctctatcttt caggttctct tttactcagt atacgcttat gtatttatag ccatactacc
     2221 cggctggttt ggaataccca ccaacaactc tgttgaacaa attaccattg gccaaatagc
     2281 tgaaagtgtt tttatttacc ttgggatacc ttttattgct ggttttatta cacgttttac
     2341 cctagttagg ttaaagggcg ataattggtt taacaccaaa tttataccta aaataagccc
     2401 attaacttta attgcgttac tctttacgat ttttgtaatg ttctccctta aaggggaata
     2461 catagtaaaa atccccatgg atgttgtgct tattgctatt cctctgctcg catatttcgt
     2521 aataatgttc cttgtatcct tcttcatgag ccgaaaagca ggtgccacgt acgagcaaac
     2581 caccactctc tcctttactg ctgctagcaa caattttgag cttgccatag ccgttgccat
     2641 tgctgttttt ggtatcagtt caggcgaagc ctttgctgcc gtaattggcc cgctggttga
     2701 agttcctgtg atgattggac ttgtaaatgt tgcattctac ttcaaaaaaa gattttttta
     2761 acccacaaaa agctatataa ggcccgctat atgggcgggc tttttttatt tttactgtat
     2821 ggaaattgag cgtaaatttt tggttgatct ccctaaatgg gagaaactag acaaaccaaa
     2881 ccctataaga attgtacagg gttatctgag cacaaaccct gagcaaacta taagggtaag
     2941 agtttgggcc gataaagcct ttatgaccat taaaggcaaa gttcaaggga tatcccgcga
     3001 ggagattgag tttgaaatac ctaccggtaa ggccaatgaa ctgcttacca agttttgtaa
     3061 agcagttatc aaaaaaaaca gatacctaat tgttcataat agcaaaacct gggaggttga
     3121 tgtgtttgaa ggcgaaaacg ctggactagt acttgcagag attgaacttg gctcagagga
     3181 cgaaaaattt acggtacccc actggataac cattgaagta accgacgatt atagatatta
     3241 caattcgtat ctatctgaaa acccatactc tagctggaaa aaatgattga ccatgataat
     3301 cattgagata cccggtagaa aaactctacg gattgagcac ttggtgctcg actttaacgg
     3361 cactcttgcc attgatggca aattgattga aggggtaaga gaacgcttgc agcaattatc
     3421 aactcagctg atggttcatg tgattaccgc cgataccttt ggtactgcaa cccacgaact
     3481 aagaggtatt gactgtaaag ttaaggtact tgaaaagggg cggcaggaca agcagaaaaa
     3541 aatctacata aatgagcttg ggaaaaataa atgcgccact attggcaatg gttacaacga
     3601 ttggcaaatg ctaaagcatg ccgccttgag tattgcaaca attcaagcag aaggtgcttc
     3661 aacgctttcc ctgaagcagg cttcaattgt atgctacaat gtaaaccacg cactcgatct
     3721 tttacttaat cccaaccgtt tggtagctac tcttagaagg taaacgcaat taatcaatcc
     3781 aactccagca tttgtcaacc attagtcctt ttcggtaaaa aacacaccaa aaaggagcct
     3841 taaatttgtt aataaacgta aaaataccat atattacacg catgaaaaac taataggagt
     3901 tggcatcgaa ggttgcgtta cttattatta atcagattct tccggttgat aaatttgaaa
     3961 caatactgga agcaaagggt tattcgtgcc taaccttgga tagaatttcc tccgatattg
     4021 agctacctat ctttaatatt gccatagtag gtctggacaa tgaaaaaacg tacctaaaac
     4081 taaaagagaa aactgacgac tacgacgggg tgatactttt cttttccgga aaacattaca
     4141 atgaaattgc cagccaaata gcaaaactgc ctgagagttt acacattgag cctaattttt
     4201 cagggaaaga actagagctc gctattcagc tacagctgct caaaaagcag atacaaaaag
     4261 ctgaaagttt tgccagcagc aactttgaca caaaccatgt caggcttaaa aagattataa
     4321 ccgaggaaca ggcttggaga ataatctttg agcaatcgcc taacggcgtt ctcctcagcg
     4381 acgatgaagg gaacataata tacaccaatt ctgccgctac cgatatttta ggctacactc
     4441 gcgacgagtt cctaaaaatg cgctttcacg accttgcgcc caaggagcta gcggagcagg
     4501 tagataccaa tatcaaaaag atactgcgag gcgaatccct aatcactgag gtttataaca
     4561 ttcgaaaaga cggcagtaag cgctatgtcc aacttcacga aaccagggtt atttttccaa
     4621 acgggaagta tggcataatg gttatttcaa ccgatataag ccgagccaag caagccgaag
     4681 aggcccttaa ggaatcgatg gagatgtata ggattctagt tgaacactcc aacgatggta
     4741 ttgtatttgt aaaggaagga atcgtaatat acgggaatcc ccgtatcatt gagattctaa
     4801 acgccaaacc cgaagagttc cttaaccaac caataactaa gtttatacat ccaagccaaa
     4861 aagaatttct gctccagcga tataaaaacc gccttgcggg taaatctgag ccaacaattt
     4921 acgaaaccat tctcgtgtcg agccagaatg aggaaattaa tgttgaattt aacgtaaatt
     4981 tagtcgatca ggcgggtgaa aagatcgata ttgttttcat ccgcgatatc accacaagaa
     5041 aacaggccga aaaggccata aaagagagcg aggaaagcta caagggatta ttcaacaact
     5101 ccagcgaagg tattcttatt attgactcaa gcggcgtaat tcttgatgcc aatagaagcg
     5161 gacaaatact cttcggatac agcaaggatg agctactcgg aaaaacaatt catgaaatta
     5221 ccgataatac acaaacatcg ctcgaggagg ttacccgttc ggtaaggctt gcataccagg
     5281 gtcataccca aaaaatggaa ttttggggga ttaggaagaa caatgatctt gttcctattg
     5341 atatggtttt taaccgaggc gactactttg ggctatcggt tatttttgcc atgtgtcacg
     5401 atataaccga ccggaaaaat gccgaggcag tgctccgcga aagcgaggaa aagtaccgta
     5461 cacttactga gcagattcct gtgggtcttt accgaattct tagcaacggg caaattgttt
     5521 actgcaatcc cgcatttgct gaaatttttg ggtttagtag ttcccaagcg gccaccggca
     5581 gtaatattaa atcgttcctt agagattttg acaagtggtt ggttgactgc tcaactgcca
     5641 agttgccaat tgaggttgaa atgttgaggg ccgacggctc caccatatgg gttcaaaata
     5701 ttctccagcc caatgttacc gaaaaccccg atgccacata ctttgatggt atgctaacag
     5761 acgtaaccga tcgtaaagtt gccatagatg agcttactga aagcgaggct aagttcagag
     5821 ccatgctctt agcaatacct gaccatctgt tccggcttaa ccgcgaaggc atccttatcg
     5881 actttgcccc caccgatata gcattttatc cggaaatatc taacctacac ctgggcaaaa
     5941 gcattgcgga aattttccca acatccatat acgaaaaata ctccgaggcg cttaactatg
     6001 cttcaataac cgggaaactt caaaggtttg agtactcgtt caggcaagaa aattccatat
     6061 tttactacga ggctcgcata gttccggccg gtgaaaagat atttctggtt ttgctgcgcg
     6121 atatcaccca acaaaagaaa tttgaggagg agatacggat tctggcacaa accataatga
     6181 atgccaacga cagcatatca atcactgacc tcgatggaaa tttcatatac gtcaatcctg
     6241 ccttcactaa agtttatggc tatagccaaa cggaaatact gggaaaaaac attaacttaa
     6301 tcagaccaat aggtttcgac tctgacctca gcaaagagat tcattcagct acccaaaaag
     6361 ggggatggaa aggcgagatt ctaaatgtac ggaaggatgg aacaatcttc ccaatattcc
     6421 tctccacctc acctgtatac aacgagttca acgagccaat tgcatcggtg ggaattgcta
     6481 acgacattac tgagcgcaag ttaattgaac aggagcttat aagggctaag gaacaggccg
     6541 aggagtcgga caggttaaaa acggcgttcc tctcaaacat gagccacgaa atccgctcgc
     6601 ccatgaatgc agtgcttgga tttatccaac tgcttaaagc agaggaaagt attactgaaa
     6661 ccggaaagca atacattgaa ctgattgaaa atagcggaaa ccagcttctt gccttaattg
     6721 aagacattat tgatatctca aagattcagt ccaaccaaat aagaatcaac aaaacttcat
     6781 tcgattttaa cgagctttta cgggaacttt acaccattta ctccaaccag ctcatacgga
     6841 aagccgatca aaaaaccctc ctgtttaagc ccgaaatgcc acaggaatca ccatttacta
     6901 tatactccga tcccgtgagg gtaaggcaaa tccttaacaa tttactgagc aatgccatta
     6961 agtttacccc agctggccat atccgatttg gatataccct catgatagat gaagatatgc
     7021 cctacattca gtgctatgtt caggatactg gaattggcat atcgcccgaa aaacaggccc
     7081 ttatttttga gcgtttccgg caagccgacg atacctatac ccgtatttac ggtggtagcg
     7141 gattaggatt agccatatcc aaaggattag tcgacctgct aggtggacat atttgggtag
     7201 aaagcactga gggtcaaggc tcaacattct acttcacaat tcctttccag gaaggcactg
     7261 agagtggtaa gagcttaaaa aaagaggaac aaaaaaccct gtcgtcggag ttgctaaaag
     7321 gcaaaaagat attagttgtg gaggatatca gtgagattag gctatacttt gagcgcttac
     7381 ttgaaccaac cggtgcaaaa actctttttg cacataatgc acgcgaggca cgagtgctat
     7441 tcaaaaagca taaggatatt agcgttgttc tgctcgacat caggcttccc gatgccgatg
     7501 gctacacaat agctgcggag ttcaaacgcc tgcgccccga tttgcccatt attgcccaaa
     7561 cagcatatgc actccagtcg gagtttaaca agagtataga gagcggctgc gacgattatg
     7621 taaccaagcc catcgactca cccctgctca tccaaaaaat attaagctta atagaaaaga
     7681 aatccgataa tcactaagga gctaagcgct caattttcca ttgattatta atcagtgtgt
     7741 actgtatcct atcgtgtaaa cggttaggac gcccctgcca gaattcaaaa agtgtaggta
     7801 ctagagtgta tccaccccag ttgggtgggc gttttacggt tttcccttta aattcttcct
     7861 caaaatcggc cattaagcgt tccaggtact tgcggttggg aatcactgtg ctctgaggcg
     7921 aagcccaagc tccaatttta ctggcctcgg gtcgtgtctt aaaatacctg tcgctttcgg
     7981 catcggttgc cttatgtatt ttaccctcaa tccttacctg tcgctc
//
LOCUS       DAOSAL010000094         7612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_6227_length_7612_cov_46.173482, whole genome shotgun sequence.
ACCESSION   DAOSAL010000094 DAOSAL010000000
VERSION     DAOSAL010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 7612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7612
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6227_length_7612_cov_46.173482"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1149)
                     /locus_tag="PK049_10395"
     CDS             complement(<1..1149)
                     /locus_tag="PK049_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK62009.1"
                     /translation="MKTFIKATLLSAWSIIGLLATTQAQEFSKLTPKQIGDKITLEYA
                     ISGEAVGQQFTVSPFYSIDGGKSFLPLKSVKGNVGNNIPGGRNQIIVWDVLKDLKELD
                     SDVVFKLEGVSKSVVPLQDDFRDVLFKLESLNRTPDGNVELILSITNNGPTRDLKMIN
                     GLITITDFNKRKYDAQKGKIAEVEGNERYSTPQRTIKKGENVKARFLFERVPAEVDRA
                     MRLDIGIELITDETYGIDLKIGKLQFRDLPVSTVQTTGMRVEQTKKLQLASSPEHFTI
                     RKVEVVDNNPPVIELISPANIPLVGKEATRGRPYAQSSIGMDDKRLRSLATGEELATT
                     DEFTMVKAKVTDESGIYDVTVNGISTEPSKDGVFQVKVPLEIGRNEIVI"
     gene            complement(1184..>1831)
                     /locus_tag="PK049_10400"
     CDS             complement(1184..>1831)
                     /locus_tag="PK049_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK62010.1"
                     /translation="ERFDIYFKLTAKRKVSDAMFAVLDTHVTRYNEFVVLVSYNPQRV
                     NESDTLYPLMASASAFFVSASYDGAEDNQAEYEMKNYLLTPDQKVTGAEFLFREKGNK
                     FLSVSSFNGEQLEYPAFPYSYTSPDATSRNQVLVTYNGLWSILSRQMLFTLMLNTQPY
                     SVKLKNVDQQYTSGVTRLAREIASFSSTLEINGIKFVNDTLKFDLNLKGQTNSMW"
     assembly_gap    1832..1931
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1932..3188
                     /gene="rmuC"
                     /locus_tag="PK049_10405"
     CDS             <1932..3188
                     /gene="rmuC"
                     /locus_tag="PK049_10405"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOK62011.1"
                     /translation="QRDEERIALTAKVSSLEASLQSATKTAAELRQEIEKLLVDIDTK
                     QHQLEQARYRGVELEKENIYLKESLEKQKKDLEAIGEQFTKEFKLLADQILEDKSQRF
                     TKMNAETIDRLLKPLDENIKQFQKQVQEVYEKESRERFSLGEKVKELSELNRTISQEA
                     KNLTEALKGQVKTQGNWGEMILESILSKSGLVKGREYTVQESITDEEGKRFQPDVIIS
                     YPDNRKVVIDSKVTLVAYERYCSASNPTEQQAAVNDLLQSVKRHIDGLSAKSYDELVD
                     SLDFVMMFIPIEPAFMLVMERDPAIWNYAYEKRVLLISPTNLIAALKLIYDLWQREYR
                     NRYAEEIAKKGGQLYDKFVGFLEDFRKIGDSLTSAQKKYEEAFGKLKDGRGNLVVQAQ
                     QLKELGAKAKKSIPDDFLPIDNELDE"
     gene            3264..>3859
                     /locus_tag="PK049_10410"
     CDS             3264..>3859
                     /locus_tag="PK049_10410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOK62012.1"
                     /translation="MISVCIPVYNFDVTELVGELIAQANRLQYPIEILIFDDHSHSYF
                     RNRNSQLASHSLVNYLEFDFNIGRSKIRNRLADVATGQWLLFLDCDVMVDDPNYLKRY
                     IENLPNAKVVCGGRKYGPKPFRHELLLRWKYGVKRECRSAYSRQLKPYSSFISGNFLI
                     EYDTFHSIRFNEELSGYGHEDTLLGLDLKKHKVDILHI"
     assembly_gap    3860..3959
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4065..5372)
                     /gene="der"
                     /locus_tag="PK049_10415"
     CDS             complement(4065..5372)
                     /gene="der"
                     /locus_tag="PK049_10415"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOK62013.1"
                     /translation="MGNLVAIVGRPNVGKSTFFNRLVGWRHAIVDEIAGVTRDRIYGK
                     SDWNGKEFSVIDTGGWVENSDDIFEEEIRKQVLIAIDEADIITFLVDAQEGITDMDIA
                     VAEILRRSSKPVYLVANKVDNNAIRMQAGEFYSLGLGDPWCISSINGSGTGDLLDDIV
                     KHFTDIDESAEQNEVPRIAVVGRPNVGKSSLINTFIGEERNIVTPLAGTTRDAIDTHY
                     RKFGHDFIIVDTAGLRRKTKVKEDLEFYSVMRSVRAIESSDVCLLLIDATQGIERQDM
                     NIFSLIVRNKKGLVLVVNKWDLVEKNNKTVDEYTKLIKERIAPFNDVPIIFASALTKQ
                     RVFKVIEAAMQVYENRKRRISTSELNNFLQETLQIKEPPVYKGKLVKIKYATQLPTKT
                     PQFAFFCNLPQYIKDPYKRFIENRLREKFNLTGVPIQIYFREK"
     gene            complement(5415..6296)
                     /gene="era"
                     /locus_tag="PK049_10420"
     CDS             complement(5415..6296)
                     /gene="era"
                     /locus_tag="PK049_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOK62014.1"
                     /translation="MSHKSGFVNIIGNPNVGKSTLMNALVGERISIVTSKAQTTRHRI
                     MGIVNSDDFQIVYSDTPGILKPAYKLQSAMMRFVESALEDADVILYITDTVETPNKNQ
                     EFLDKIQQLDIPIILAINKVDLSTQEKVSLLIEQWGQMMPKADILPISALHGFNTDAL
                     VNMIVERLPEGPEYFPKDTLTDKTLRFFAAEIIREKILLNYKKEIPYSVEVEIEEYKE
                     SETIDRIRAIIYVDRESQKSIIIGSQGRMLKKVGTEARLELETFLGKKVFLEIYVKVD
                     PNWRNDEGKLKRFGYPL"
     gene            complement(6316..7521)
                     /locus_tag="PK049_10425"
     CDS             complement(6316..7521)
                     /locus_tag="PK049_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005804197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOK62015.1"
                     /translation="MRKESMNRDVKREKRSNSPEEPARVYRPRTLTLKKVDANDQSDS
                     KPEKRVSPRSDEHKPEPKRRSEDSRRGDFRKPKSDNSEQPKSYRPRSWKKDSDSREHE
                     PRETSHERSSRPSRDERKHESGRKNDGFKLVKHTRYNESKSKEYTRDNAKAEGMVAKK
                     TADELIRLNRYIANSGVCSRREADELIANGMVTVNGKVVTELGTKVRRDDVVMYKGKR
                     LTAERKVYILLNKPKDYVTTVDDPHAKHTVMELIEGACDVRVYPVGRLDRNSTGVLLL
                     TNDGDLTTRLTHPSFKKRKIYHVGLDRNVRPEDMNKIFEGVELDGEKIQVDAIDYVDG
                     SDGSEVGIEIHSGQNRVVRRIFESLGYKVIKLDRVYFAGLTKKNLPRGKWRFLTEKEV
                     NMLKMNRFY"
BASE COUNT         1892 a         1779 c         1726 g         2015 t
ORIGIN      
        1 gattacaatc tcgttcctgc ctatctccaa aggaacctta acctggaata ctccatcctt
       61 tgagggctcg gtagaaatac cgttaacggt tacatcgtat atcccgctct catcggtaac
      121 cttggcctta accatggtaa actcatcagt ggtggcaagt tcctcaccgg tagcaaggga
      181 gcggagccgc ttatcgtcca tcccaatgga cgactgtgcg taggggcgac cgcgagttgc
      241 ctctttgcct acaagcggga tgttggctgg cgaaataagc tcaattaccg gcgggttatt
      301 atcaactacc tccaccttgc gaatggtaaa atgctcaggc gatgacgcta gctgaagttt
      361 tttggtttgt tcaacacgca tccctgtagt ttgaacggta ctaacgggaa ggtcgcggaa
      421 ttgtagtttt ccaattttca ggtcaatacc gtaagtttca tcggttatga gttcaatacc
      481 tatatcgagc ctcatggcgc ggtcaacctc ggcgggtaca cgctcaaaca ggaagcgggc
      541 cttaacgttc tcgcccttct taatggttcg ttgtggggtg gagtaacgct cgttcccttc
      601 aacctctgct attttaccct tctgggcatc gtatttacgt ttattgaaat cggtgatggt
      661 gattaacccg ttaatcattt ttaaatcgcg ggtggggccg ttgttggtaa tggagagtat
      721 cagctcaacg ttcccatcgg gtgtgcgatt taagctctcc agcttaaaga gaacgtcacg
      781 gaaatcgtcc tgaaggggca caacgctttt tgagaccccc tcgagcttga aaaccacatc
      841 ggagtcgagt tccttgaggt cttttagtac atcccaaaca attatttgat ttctaccacc
      901 gggtatattg ttccccacat tacccttaac gctcttgagc ggtaagaacg atttcccccc
      961 atcgatggag tagaagggcg aaactgtaaa ttgctgtccc acagcctcgc cggaaatggc
     1021 gtattcaaga gtaattttat cgcctatctg tttaggcgtt agcttgctaa attcttgcgc
     1081 ttgcgttgta gcaagtaaac ctatgatact ccatgctgag agtagcgttg ctttgataaa
     1141 tgttttcatt gcgccatcta ttttagtttt acatataaag cttttaccac atgctattgg
     1201 tttgaccctt aaggttcagg tcaaacttca aggtatcatt tacaaatttg atgccgttaa
     1261 tttcaagggt ggatgaaaat gatgctattt cacgtgctaa ccgggtaacg ccgctggtgt
     1321 actgttggtc aacatttttc agcttaacgc tgtagggctg agtgtttagc atcagggtaa
     1381 aaagcatttg cctcgataga atgctccata aaccattgta agttaccaga acctgattcc
     1441 gcgatgtggc atcgggcgat gtgtaggagt aagggaatgc cggatattca agctgctcac
     1501 cgttaaagct cgatacgctt aggaacttat tacctttctc cctgaatagg aattcggcac
     1561 cagtaacctt ctggtcggga gtaagcaggt agtttttcat ttcgtactca gcctggttat
     1621 cctcagctcc atcgtagctt gccgaaacaa aaaacgccga tgcgctggcc attagtgggt
     1681 aaagagtatc ggactcatta acccgttgtg ggttatagct aaccagcacc acaaactcgt
     1741 tatagcgtgt tacatgtgtg tcgagcacag caaacatagc atcggaaacc ttgcgtttgg
     1801 ctgtcagctt aaagtagata tcaaaccgct cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn ncaacgcgat gaggaaagaa tagcgctaac cgcaaaggtt tcatcgcttg
     1981 aagcatcgct acagagtgcc actaaaacag ccgctgaact gaggcaggaa attgagaagc
     2041 tactggtaga catcgatacc aaacagcatc agcttgaaca ggcacgttac aggggagtag
     2101 agcttgaaaa ggagaatatc tacctgaaag aatcacttga aaagcaaaag aaggacttag
     2161 aggcaattgg tgagcagttc accaaggagt ttaagctcct ggccgaccaa atccttgagg
     2221 ataaatcgca acggtttacc aaaatgaacg cggaaaccat cgataggctg ctgaagccgc
     2281 tcgacgagaa tataaagcag ttccaaaagc aggttcaaga ggtttacgag aaggaaagcc
     2341 gtgagcgttt ctcgctgggc gagaaggtta aggaacttag cgagcttaac cgaaccatca
     2401 gccaggaggc caagaaccta accgaggcgc tgaaggggca ggtgaaaaca cagggaaact
     2461 ggggcgaaat gattcttgag tccattctat ccaaatcggg gctggtaaag gggcgtgagt
     2521 acaccgtgca ggagtcgata acggacgagg aggggaagcg ttttcagccc gatgttatca
     2581 tttcataccc cgacaaccgt aaggtggtga ttgactcaaa ggtgacactg gtggcctatg
     2641 agcgttattg ctcggcatcg aacccaacgg agcagcaagc tgctgtaaac gatttgctac
     2701 aatcggttaa gcgacatata gatggactat cggcaaaaag ctatgacgag ctggttgatt
     2761 cgctcgattt tgtaatgatg ttcatcccaa ttgaacccgc atttatgctt gtgatggaac
     2821 gcgacccggc aatctggaac tatgcctacg aaaagcgtgt tttgcttatc agccctacaa
     2881 acctgattgc tgctttaaag ctgatatacg acctgtggca gcgcgagtac cgcaaccgtt
     2941 acgccgagga aattgccaaa aagggcggcc agctctacga taagtttgtg ggtttcctgg
     3001 aagatttccg taaaataggc gatagcctca catctgctca aaaaaaatac gaggaagcgt
     3061 ttgggaagct aaaggatggc aggggcaacc ttgtggttca ggcacagcag ctaaaggaac
     3121 taggagcaaa ggcaaagaag agtatccccg acgatttcct gcctattgat aacgaattgg
     3181 atgagtaagg caaacaaaaa tttcgatttc atcgtattac aatgaggcaa tttgtgttac
     3241 tttagcagtt taattggctt agaatgattt cggtttgcat tccggtttac aatttcgatg
     3301 ttaccgagct ggttggtgag cttattgctc aggcaaaccg gttgcaatac ccaattgaga
     3361 ttctcatatt cgacgaccac tcgcacagct acttccggaa ccgcaactcg cagcttgcct
     3421 cgcatagctt ggtcaactac ctggagttcg atttcaacat aggccgttca aaaattcgca
     3481 accgcctagc cgatgttgcc accgggcaat ggttgttatt cctcgactgc gatgtaatgg
     3541 ttgacgaccc gaattacctt aaacgttaca ttgaaaatct acctaacgca aaggtggtat
     3601 gtggcggacg taagtatggc cccaagccgt tccgtcacga gcttctgctc cgttggaaat
     3661 acggcgtaaa acgcgaatgc cgctcagcat actcccggca gctaaagcca tacagctcgt
     3721 ttatcagcgg aaacttcctg attgagtacg acaccttcca cagcatccga ttcaacgagg
     3781 aactatcagg ctacgggcac gaggacaccc tccttggcct tgacctaaaa aagcataagg
     3841 ttgatatcct ccatatcgan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
     3961 gcggtagccg tccatcaatc ctgctgatgg acttgtacaa gctaacacta ctggcaaagt
     4021 tgtatacaaa aggctggcgt aaccaaagcc agccctgaga aatgctactt ctccctaaag
     4081 tatatctgaa taggtactcc ggtaaggtta aacttttccc ttaacctatt ttcaatgaat
     4141 cgcttgtaag gatcctttat atactgcggc aagttgcaga aaaatgcaaa ctgtggagtt
     4201 ttggtgggta actgcgtggc atacttaatt ttcacaagtt tacccttata aaccggcggc
     4261 tccttgattt gcagtgtttc ctgcagaaaa ttgtttagct ccgaagtgga aatgcggcgc
     4321 ttacgatttt cgtaaacctg catggcggct tcaatcacct tgaaaacacg ctgcttggtt
     4381 aatgccgatg caaaaatgat gggaacatcg ttgaaagggg caattcgttc cttgatgagc
     4441 ttggtgtact catcaactgt tttgttgttc ttttccacca aatcccattt gtttacaacc
     4501 agcactaaac ctttcttatt gcggactata aggctgaaga tattcatgtc ctgccgttca
     4561 attccttggg tagcgtcaat gagtaaaagg catacatcgg agctctcaat tgcccttacg
     4621 gaacgcatca cggagtaaaa ctccaaatcc tcctttacct tagttttacg tcgtaggcca
     4681 gcagtatcga caataataaa atcgtggcca aacttacggt agtgggtatc aatggcatcg
     4741 cgcgtagtgc cggccaacgg ggttacaatg ttacgttcct caccaataaa ggtgttgatt
     4801 aacgacgatt tacccacatt tggacggcca acaacggcta tgcggggcac ttcgttctgc
     4861 tcagctgact catcaatatc ggtgaaatgt ttcactatat catcgagcaa atcgccggta
     4921 ccggaaccat tgatggatga gatacaccat gggtcgccaa gacccaagct gtagaactca
     4981 cccgcttgca ttcgtatggc gttattgtca accttgttgg ccaccaggta aacgggcttt
     5041 gagctccgcc tcagtatctc ggctacagca atatccatgt cggttatgcc ctcctgggca
     5101 tcaaccagga aggtgataat atcggcttca tcaatagcaa taagcacctg cttgcgtatc
     5161 tcctcctcaa aaatatcatc ggaattttcc acccaaccgc ccgtgtcaat cactgagaat
     5221 tcctttccat tccaatcgct tttcccgtaa atacggtcgc gggtaacgcc ggcaatttcg
     5281 tcaacaatgg catgtcgcca gccaaccaaa cggttaaaga atgtcgattt acccacgttg
     5341 ggccttccta caatagcaac taaatttccc attgccctaa atatcgtttt aaatgaaata
     5401 ctaccaatat cgaattacaa ggggtaacca aagcgtttga gcttgccctc gtcgttgcgc
     5461 cagttgggat caacctttac gtaaatctcc aggaatactt ttttccccag aaaggtttcc
     5521 agctcgaggc gtgcctcggt tcccaccttt tttagcattc gtccctggct tccaataata
     5581 attgacttct gcgactccct gtccacatag attatcgccc taatacggtc aattgtttcc
     5641 gattccttgt actcctcaat ttcaacctca accgagtatg ggatttcctt tttgtagttc
     5701 agcaggattt tttcccggat aatttcggct gcaaaaaagc ggagtgtctt gtcggttaag
     5761 gtatccttgg ggaaatactc aggcccttcg ggcagcctct caacaatcat gtttaccaac
     5821 gcatcggtgt taaagccgtg cagggctgat attggaagaa tatcggcctt gggcatcatt
     5881 tgcccccact gctcaattag gagcgaaacc ttttcctgtg tggacaggtc aaccttgttg
     5941 attgccagga taattggaat atccaactgc tggattttgt ccaggaattc ctgattctta
     6001 ttgggtgttt caaccgtgtc ggtaatgtaa aggattacat cagcatcctc cagcgccgat
     6061 tccacaaacc gcatcatagc cgattgcagc ttgtaggcag gcttcagaat gcccggcgta
     6121 tcggagtaaa caatctgaaa atcgtcggag ttaacaatgc ccattatgcg gtggcgggtg
     6181 gtttgcgcct tgctggtaac aatggaaatc cgctcgccca caagggcatt cattagggtt
     6241 gatttaccca cattggggtt accaataata tttacaaaac ccgatttgtg cgacatggcc
     6301 aaagatgatt aaatcctaat aaaacctatt catcttaagc atgttaacct ctttctcggt
     6361 aaggaagcgc cacttgccgc gtggcaaatt cttcttggtt aggccggcaa agtaaaccct
     6421 atcgagcttt attaccttgt agccaagtga ctcaaagata cgacgcacaa cgcggttttg
     6481 gccgctatgg atttcgatac ccacctcgga gccatcgctt ccatcaacgt aatcaatggc
     6541 atctacctga attttttcgc cgtcgagctc aaccccctca aatattttgt tcatatcttc
     6601 cggacgaaca ttgcggtcga gtcccacatg gtaaatttta cgctttttaa agctggggtg
     6661 tgtcagcctg gtggtgaggt caccatcgtt ggtgagcaga agcacgccag tgctgttgcg
     6721 gtccagccta cctacggggt aaacacgaac atcgcaggcc ccctcaatga gctccatcac
     6781 ggtatgcttg gcatggggat cgtcaacggt ggtaacgtaa tccttgggtt tgttaagcag
     6841 gatgtaaacc ttgcgctcag cggttagccg cttgccctta tacataacca catcatcgcg
     6901 gcgaaccttg gtacccagtt cggtaaccac cttgccgtta acggtaacca tgccgttagc
     6961 aatcagctca tcggcctcgc gacggctgca cacgcctgag ttggctatat acctgttaag
     7021 gcggataagc tcatcggctg tttttttggc aaccatgccc tccgcctttg cgttatcgcg
     7081 ggtgtactcc ttgcttttgc tttcgttgta tcgggtatgt tttaccagct taaagccatc
     7141 gtttttccgt ccactttcgt gcttacgctc atcccgtgat ggtcttgagc tacgctcgtg
     7201 tgaggtttcg cgcggctcat gctccctgga atcggaatcc tttttccatg agcgtgggcg
     7261 gtacgatttt ggctgttcgg aattgtcgga ttttggtttg cggaaatcgc ctcggcggct
     7321 atcctctgac ctgcgctttg gttccggttt gtgttcatcg gaacggggtg atactctttt
     7381 ctcgggctta gaatcgcttt ggtcgttggc atctaccttt ttcaacgtaa gggttctagg
     7441 cctgtaaacc ctggcgggct cctcggggga gttactccgc ttctcgcgtt taacatccct
     7501 gttcatactc tcttttctca tttatatgct taattattaa ctatttacgg ctaacgtcat
     7561 agccatttag gcttgcaaag atacaaacaa aaattgattt acatgctgat ta
//
LOCUS       DAOSAL010000095         7573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_6285_length_7573_cov_49.581538, whole genome shotgun sequence.
ACCESSION   DAOSAL010000095 DAOSAL010000000
VERSION     DAOSAL010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 7573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7573
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6285_length_7573_cov_49.581538"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..1219)
                     /locus_tag="PK049_10430"
     CDS             complement(23..1219)
                     /locus_tag="PK049_10430"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOK62016.1"
                     /translation="MKRIFSRVVITLSLVSFFTDIASEMLYPVMPIYLKSIGFTALLI
                     GILEGIAEATAGLSKGYFGELSDRLGKRVIFIRWGYFLSALSKPTMAISKWPVWIFIV
                     RTTDRMGKGLRTSARDALLSDESTPETRGKVFGFHRGMDTLGAAVGPAVALLLLWLYP
                     GNYTYIFLITIIPGLAAVLLTFLLKEKAKEHIHSNSKGIVSPFSFLKYWNQSPINYRH
                     IVIGLIIFTLINSSDAFLLLKVKEEGASDTLVIGLYIFYNLAYALLSYPMGWLSDKLG
                     MKRMVIFGIAIFAITYLGFALTSKWSVFILLFGLYSLYAASTEGVLKAWLANNLPKTK
                     TATGIGLFNSLQSIATLLASSLAGLVWVMAGSGIVFMISAVGATLAGIYLYIFTRTSV
                     TYQGSE"
     gene            1396..>2171
                     /locus_tag="PK049_10435"
     CDS             1396..>2171
                     /locus_tag="PK049_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005818638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOK62017.1"
                     /translation="MLVLTGIQAFSQAGWNTPVPVDPNVRYGKLENGLTYYIRKNTEP
                     KQRAEFYIAQNVGAILEEDSQNGLAHFLEHMAFNGTKNFPGKGIINYFETVGVKFGFN
                     INAFTSVDETVYNLSDVPTTREGIIDSALLVLHDWSHFISLLPEEIESERGVIREEWR
                     TGRNAERRMYKQLMPVMYKGSKYAERDVIGDINVINNFKHQELVDYYNKWYRPDLQSI
                     VIVGDVDVDQVEAKIKSLFADIPAPVNPAPRPFYELPDND"
     assembly_gap    2172..2271
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2272..3849
                     /locus_tag="PK049_10440"
     CDS             <2272..3849
                     /locus_tag="PK049_10440"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOK62018.1"
                     /translation="WECVDHFLKGEPMPGIEFEYMFASQLMPTITIDEINAVAKQLIT
                     DKNVIISLMAPQKDGITVPNQEQLLAALKEVKAENIEAYVDKVITKPLVEKIEKPGKV
                     KKENVKNVYGTTEWILSNGVKVVVKQTDFKEDQVVVDAFSAGGVSLASIDELPSASMA
                     TQIITNAGVGEFTSTDLEKMLAGKMVNVRPVIAESYEGFSGEASPKDFETLLQLIYLY
                     MTAPRIDEENFNTYMGRMKAFMANASSDPRMAFRDSITVALANHNPRVMPMNLDYLNK
                     VDFNKSINFYKNRFADASDFTFIFTGNVSPVEIKPLVEKYLGALPTVKRKDVAKDNGV
                     RPPKGKVQNYFTKPMQTTKSSVFVGFTGEVEYNVMNDILADYLTSILRTRYLEEIREK
                     EGGTYGVGVRVSLRKFPVNSFVVQIQFDTDPKLRDKLIGIVYSEIEKIKTNGPLADDL
                     NKSKEFMLKEYEQNQRENSYWTNVIRAKYEDGLDFDSKYVEMVKAVDVNAIKEFANKI
                     FGQGNVVEVVMTSEEQK"
     gene            3944..5068
                     /locus_tag="PK049_10445"
     CDS             3944..5068
                     /locus_tag="PK049_10445"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOK62019.1"
                     /translation="MRPLLSLVLTIICSIGFAQYDSTLRLIFIGDVMGHVPQIQSAYD
                     SSTGRYDYTDVFSELKGLLTSADYSIANLEVTLGGEPYTGYPQFSSPDELVDGLMAAG
                     VNVLVTANNHSVDRGGDGIRRTIDVLAAKNLLFTGTFLDSTDRSIRNPIVLNKNGVKL
                     ALLNYTYGTNGIPVPSPYLVNMIDTVSMANDIARAQALGVDDIVVFIHWGNEYERLPN
                     MQQKRLTGWLHGKGVRIIIGSHPHVVQPAEFNRFENDFRLVVYSLGNFVSNQRRRYCD
                     GGVVVLVDLMKQNGKLEVSGVGYIPVWVDTYFSSGKRKYKVFPVGMYEGKSNQFLSAA
                     SDSAFKVFVDDTRSLLNANNINFPEVKLVNGEWVVPLLSR"
     gene            complement(5058..5777)
                     /locus_tag="PK049_10450"
     CDS             complement(5058..5777)
                     /locus_tag="PK049_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HOK62020.1"
                     /translation="MVPTWLERTELLLGGKAVEKLSQKHVLIIGLGGVGAYAAEQICR
                     AGIGALTIADGDTIHESNINRQLPALHSSVGQPKAEYIGKRLLDINPNLKLTVIQEYL
                     RDERLTALLSHKYDYVVDAIDTLSPKVFLIHDALRFGQRVVSSMGSGGKLDPTMVRIS
                     DISESHGCPLARILRKRLHRLGIRQGVKVVYSPEEVPENATRPCEGEPNKKTTIGTIS
                     YMPPLFGCMIASVVVRDLLND"
     gene            complement(5833..6513)
                     /locus_tag="PK049_10455"
     CDS             complement(5833..6513)
                     /locus_tag="PK049_10455"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOK62021.1"
                     /translation="MKLVNFHTHKPQFNSNSICVVPYDIDGFNLYSSSHYKTIGLHPW
                     KTESSEISQWLGKLNELAKQPDFIGIGEIGLDRLKGAPLKNQIDIFEKILQTANEFKK
                     PIIIHCVRCWTELLAILSKSKYRELPKAIHGFRAKADVAKQLVDNGFYLSFGTALIHA
                     TPELAEALTLVPRNKLFLETDDTEIPIYEVYDAAADILDSTIEELVSVTNQNLLTFFG
                     NTLSDKSK"
     gene            complement(6545..6817)
                     /locus_tag="PK049_10460"
     CDS             complement(6545..6817)
                     /locus_tag="PK049_10460"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOK62022.1"
                     /translation="MKTIAITVKGKVQGVGFRYFVMRHANEIGVNGFVKNLPNGDVYI
                     EASGTNSQLEQLILLCNQGPAHSNVENVNVKDIQQSFDIIGFQILG"
     gene            6872..7363
                     /locus_tag="PK049_10465"
     CDS             6872..7363
                     /locus_tag="PK049_10465"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HOK62023.1"
                     /translation="MILDYFLLVFSITLILLGLAGCILPVIPGPPLAWLGLLLAKFTS
                     FCSVDWITILVMAGVTLIVTLLDYLFPAWIAKKMGGSNWGAWGTIIGLLVGVVFLGPL
                     GAVVGSFVGALVGEMLRKQPQNNTKNNPWLSAVGSFLGFMLGVGVKLITVGVIIYFLI
                     VNF"
     gene            complement(7367..>7573)
                     /locus_tag="PK049_10470"
     CDS             complement(7367..>7573)
                     /locus_tag="PK049_10470"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOK62024.1"
                     /translation="KIITDRATGKSKGYGFVEFDNEESAQKAIKELNDTQVKGRNIKV
                     NSAFRKTDQPQASKGKEEEPDTKS"
BASE COUNT         2099 a         1485 c         1636 g         2253 t
ORIGIN      
        1 gcaattgctc gcgacgaagc gcttattcgc tcccttgata ggttacgcta gttcttgtaa
       61 atatgtaaag gtatattcct gcaagggttg cacccacagc gcttatcata aaaacaatac
      121 ctgatcctgc cataacccat actaagcctg ctaaactgct tgccagcagg gtggcaatgc
      181 tttgtaagct gttaaaaagc ccaatccctg ttgctgtttt tgtttttggg agattgtttg
      241 caagccaagc ctttaatacc ccttcggttg atgctgcata cagtgagtaa aggccaaaaa
      301 gcagaatgaa cacactccat tttgaagtta aggcaaagcc taggtaggtg attgcaaata
      361 tggctatacc aaaaatcacc atccttttca tgcccagttt gtccgaaagc catcccatag
      421 ggtaggaaag taaggcgtat gcaaggttgt agaagatata caagccaatt accaaggtgt
      481 ccgatgctcc ttcttctttt acttttagta aaaggaatgc atcggagctg ttaattaggg
      541 tgaagatgat taacccaatt acaatatgcc gatagttaat aggcgattgg ttccagtatt
      601 ttaggaacga gaaaggcgaa acgatacctt tgctattgct atggatatgc tctttagcct
      661 tttctttcag caaaaaagtt agtaaaacgg ctgcaagtcc ggggataatt gtaattagaa
      721 aaatgtaggt atagttgccg ggatatagcc agagtaaaag taaagcaacg gctgggccaa
      781 cggctgctcc gagggtatcc atgcccctat ggaaaccaaa tactttaccc cgagtttcgg
      841 gagttgactc atcgctgagg agggcatcac gggcactggt tcttagcccc ttgcccatgc
      901 ggtcggtagt tcggactata aaaatccata ccggccactt gctgatagcc atggtaggtt
      961 tggaaagggc actcaggaaa tacccccacc taataaaaat aactcgtttc cctaacctgt
     1021 ccgatagttc gccaaaataa cccttgctta atccagctgt tgcttcggca atcccttcaa
     1081 gtatcccgat aagtagagct gtaaatccaa tcgattttag gtagattggc attactggat
     1141 atagcatctc acttgctata tctgtaaaaa agctaacaag ggaaagggtg ataacaaccc
     1201 gagaaaaaat ccttttcata aattaaactt tatacttaca aaagtatctt aaggttacga
     1261 attaatgtta aaaccattaa tatttctttg aaatttttaa ttaatgtata attttgtttt
     1321 taattttatt aacaaaccta tattaaattt ttaaaagtta tgacgaataa aaaaggtttt
     1381 ttgtttatgc ttacgatgct tgttttaaca ggcattcagg catttagtca agctggttgg
     1441 aatacacctg tgccggtaga tccaaatgtt cgctatggta agttggaaaa tgggctaact
     1501 tattacattc gcaagaacac cgaacccaag caacgtgctg agttttacat tgcccaaaat
     1561 gttggtgcaa ttctggaaga ggatagccaa aatggtttgg ctcacttcct ggaacacatg
     1621 gcttttaatg gaaccaagaa ctttccaggc aaaggaatca tcaactattt cgaaaccgtt
     1681 ggtgtaaagt tcgggtttaa tataaacgcc tttacctcgg ttgacgaaac tgtttacaat
     1741 ctctccgatg tacctaccac ccgtgaaggt attatcgata gtgcattatt ggtattgcac
     1801 gattggtcgc atttcatttc attacttccc gaagaaattg agtcggagcg tggagtaatc
     1861 cgtgaggaat ggcgtaccgg tcgtaatgct gagcgcagaa tgtacaagca acttatgcct
     1921 gtaatgtaca agggctcaaa gtatgccgaa cgcgatgtta ttggcgatat caacgtaatt
     1981 aacaacttta agcatcaaga actggttgac tactataata aatggtatcg tcccgatttg
     2041 caatcaatag ttattgtggg tgatgttgat gttgaccagg ttgaggcaaa aattaaatcg
     2101 ctgtttgctg atattccagc tccggttaat cctgcgcccc gtcctttcta tgaacttccc
     2161 gacaacgatg annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntgggaatgt
     2281 gttgatcatt tcctaaaggg tgagcctatg ccgggtattg aatttgagta catgtttgca
     2341 tcgcagctta tgcctaccat tactatcgat gaaattaacg ctgtagctaa acaactcatc
     2401 accgataaaa acgttattat cagcttgatg gctccccaaa aggatggcat cactgttcca
     2461 aatcaggaac aattgcttgc tgccctaaag gaagtaaagg ccgaaaacat tgaggcatac
     2521 gttgataagg taataaccaa gccattggtt gaaaaaattg aaaagcccgg taaggttaaa
     2581 aaggaaaacg taaagaatgt atacggtacc actgagtgga ttctctccaa tggagtaaag
     2641 gttgttgtga agcaaaccga tttcaaggag gaccaggttg ttgttgatgc tttcagtgca
     2701 ggtggcgttt cgcttgcatc catcgatgag ctaccctcgg ctagtatggc cacccaaatt
     2761 attaccaatg ccggtgtggg cgaatttacc agcactgacc ttgaaaagat gcttgctggc
     2821 aagatggtta atgtacgtcc ggttattgcc gagagctacg aaggttttag cggtgaagca
     2881 tcgcctaagg attttgaaac cttactacag ctcatctact tgtacatgac agcaccacgt
     2941 atcgatgagg aaaacttcaa tacctatatg ggtagaatga aggctttcat ggccaatgca
     3001 tcgtccgacc cacgcatggc tttccgcgat agtattaccg ttgccctggc aaatcacaac
     3061 cccagggtta tgccaatgaa ccttgattac ctgaacaagg ttgactttaa caagagcatc
     3121 aacttctata agaatcgctt tgccgatgct tccgatttca ctttcatctt taccggaaat
     3181 gttagcccag ttgagataaa gcctttggtt gaaaaatacc tgggggcatt gcccaccgtt
     3241 aagcgtaagg atgttgccaa ggataatggt gtaagaccac ctaaaggcaa ggttcagaat
     3301 tactttacaa agccaatgca aaccaccaag tcgtcagtat ttgttgggtt taccggtgag
     3361 gttgagtata atgtgatgaa cgatatactt gccgactacc tcacatccat attacgcacc
     3421 cgttaccttg aggaaattcg tgaaaaagaa ggtggtactt atggtgttgg agttagggta
     3481 tcgctccgta aattccctgt aaactccttt gtggttcaga tccagttcga taccgatcct
     3541 aagcttcgcg ataagctaat tggtattgtt tattctgaga ttgaaaagat taaaaccaat
     3601 ggaccattgg ccgatgatct gaacaagagc aaggagttca tgctcaagga gtacgagcaa
     3661 aaccagcgcg aaaactctta ctggacaaat gtgattaggg caaaatacga ggatggcctt
     3721 gattttgact caaagtatgt ggagatggta aaggctgttg atgtgaatgc cattaaggaa
     3781 tttgccaata agatttttgg acagggtaat gttgttgagg tagtaatgac ctccgaagaa
     3841 cagaaatagc attaggtttt tcaatacaaa ggggtatccg taatgggtac cccttttttg
     3901 ttgataggaa agtttttcct atcattgtac aaacatcata gttatgagac ctctactatc
     3961 cctggtgcta actattatat gcagtattgg ttttgctcaa tacgatagca ctctacgctt
     4021 aatttttatt ggtgatgtaa tgggccatgt tcctcaaatc caatcggcat atgatagttc
     4081 aaccgggcga tacgattata ccgatgtttt ttctgaatta aaaggcttgc tcactagtgc
     4141 cgattattcc attgccaacc tcgaggtaac tttaggagga gaaccctata ccggttaccc
     4201 tcagtttagc tcacccgatg aacttgtcga tggtttaatg gctgcaggtg ttaatgtttt
     4261 ggttaccgcc aataaccatt cagttgatag gggaggggat ggtataagga gaaccattga
     4321 tgttcttgcg gccaaaaact tactttttac agggacattc ctcgattcaa ctgacaggtc
     4381 aattcgaaat cctattgttc taaataaaaa tggggtaaag cttgccttgc ttaactatac
     4441 ctatggaacc aatggaattc cagtcccaag cccatacttg gtcaatatga ttgatacagt
     4501 tagcatggca aatgatattg ctagagcaca agctctagga gtggacgata ttgttgtgtt
     4561 cattcactgg ggcaatgagt acgaacgttt acctaacatg cagcagaaac gattaactgg
     4621 ttggctgcat ggtaaagggg tacgtattat tattggttcg cacccccatg ttgttcagcc
     4681 cgctgagttt aaccgtttcg agaacgattt taggctggtt gtttactcgc ttggcaactt
     4741 tgtttccaac cagcgtcgaa ggtactgcga tggaggtgta gttgttcttg ttgatttaat
     4801 gaagcaaaat ggaaagctag aagtaagtgg tgtgggatac attccggttt gggttgatac
     4861 atacttttca agtggaaagc gtaagtataa ggttttcccg gtggggatgt atgagggcaa
     4921 aagcaatcag ttcctttcag cagcaagcga ctccgctttc aaggtttttg ttgatgatac
     4981 tcgtagtttg ctgaacgcca acaatataaa tttccccgaa gtaaagctag taaatggcga
     5041 atgggtggtg cctttgctta gtcgttaagc aaatccctaa caaccactga ggcaatcatg
     5101 caaccaaaga gtggcggcat gtacgaaatg gttccaatgg tagttttctt attgggttca
     5161 ccctcgcagg gccttgtggc attctcggga acttcctcgg gcgaataaac aaccttaacc
     5221 ccctgacgta ttcccaagcg gtgtagccgt tttcttagga ttctggcaag tgggcatcca
     5281 tgcgattcag aaatatcgga aatccttacc attgttgggt cgagttttcc tccgctcccc
     5341 attgaactta ccactcgctg tccaaaccga agggcatcgt gaataaggaa aaccttaggc
     5401 gaaagagtgt caatagcatc tactacgtaa tcgtatttat ggcttagcaa tgcagtaagc
     5461 cgttcatccc ttaggtactc ttgaattacg gttagcttta ggtttgggtt aatgtccagt
     5521 aaccgtttcc caatatactc tgcttttggt tggccaacac tactatgaag tgccggcaat
     5581 tggcgattaa tgtttgattc gtggatagta tcgccatcag caatggttag tgcaccaatt
     5641 cctgcacggc agatttgttc ggctgcgtat gctcctactc ctccaagccc tataattagc
     5701 acatgctttt gcgatagctt ttcaacggcc ttgccgccaa gtaatagttc agttcgttca
     5761 agccaggtgg gaaccatatc atgttttttg ttcagcaaag gtcgtaatca gtatcattaa
     5821 atccaataac ctttacttgc ttttatcgga aagggtgttc ccaaaaaatg ttaacaggtt
     5881 ttggtttgta acactaacaa gttcctctat ggtggagtca agaatatcgg cggctgcgtc
     5941 gtaaacctca taaatgggta tctcggtatc gtcagtttca aggaatagtt tatttcttgg
     6001 aacaagcgtt aatgcctcag ctaactctgg tgtagcatgt ataagagcgg ttccgaatga
     6061 aaggtagaat ccattgtcaa caagttgttt ggctacatcg gctttggctc taaagccatg
     6121 tatggcttta ggtagttctc tatattttga ttttgataga attgctaata gctcagtcca
     6181 gcatctaaca cagtgaatta ttattggctt tttaaattcg ttggcagttt gaagaatttt
     6241 ctcaaaaata tcgatttgat tctttaaagg agcgcctttt agcctatcta accctatttc
     6301 ccctattcca ataaaatcag gctgcttagc cagctcattt agcttgccaa gccattggct
     6361 aatctcactg ctttcggttt tccaggggtg taaaccaatt gttttgtagt gcgatgaact
     6421 ataaaggtta aaaccatcaa tatcgtaagg cacaacacaa atgctattgc tgttgaattg
     6481 aggtttatgt gtgtgaaagt taaccagctt cattgttaaa ttctttatgt ggttttgaac
     6541 taagttaacc aagtatttgg aatcctataa tatcaaatga ctgttgaata tctttaacat
     6601 tgacgttttc tacatttgaa tgtgcaggtc cctggttgca cagtaaaatc agttgctcaa
     6661 gctggctgtt tgttccgctt gcttcaatgt aaacatcgcc attgggcaag ttcttcacaa
     6721 aaccattaac accaatttca ttggcatggc gcattacaaa gtatctaaaa cctacaccct
     6781 gaacctttcc ctttaccgtt attgcaatgg ttttcattct aggaaaaaat gatatattta
     6841 ccaaagttat tgaataagaa caaaaaagca aatgatacta gattactttt tgcttgtatt
     6901 ttccattacg ctaatactgc tgggactggc agggtgtatt ttacctgtaa ttccagggcc
     6961 gccgctagcg tggctggggc tgttgctggc aaagtttaca tcgttttgct ctgtggactg
     7021 gatcaccatt ttggttatgg caggagtaac tctaattgta accctgctcg attacttgtt
     7081 ccctgcctgg atagccaaaa aaatgggtgg aagtaattgg ggtgcctggg gtacaatcat
     7141 tggcctactt gttggggtag tatttttagg gccacttgga gccgttgttg gatcttttgt
     7201 aggagcgctt gttggcgaga tgctaaggaa acaaccccaa aataatacca aaaataatcc
     7261 ctggctttcg gcagtaggat catttcttgg atttatgtta ggagtaggtg taaagctaat
     7321 tacagtaggt gtaataatct actttttaat agttaacttt taatttttag gatttcgtat
     7381 cgggctcctc ctctttaccc ttacttgctt ggggttgatc ggtttttcgg aatgcgctat
     7441 taaccttaat attccgaccc ttaacctgag tgtcgttaag ttcttttatg gctttctgag
     7501 ccgattcttc attatcgaac tcaacaaaac cgtagccttt cgattttccg gtagcacgat
     7561 cggtaatgat ttt
//
LOCUS       DAOSAL010000096         7560 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_6299_length_7560_cov_51.890073, whole genome shotgun sequence.
ACCESSION   DAOSAL010000096 DAOSAL010000000
VERSION     DAOSAL010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 7560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7560
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6299_length_7560_cov_51.890073"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..3382
                     /locus_tag="PK049_10475"
     CDS             68..3382
                     /locus_tag="PK049_10475"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOK62025.1"
                     /translation="MKKLLFLGILFISVLQASGQQFNKYGNPLITNYTPEVYGGNEQV
                     WCIAQDDRGVMYFGTNDNGILEYDGKTWRSIPMPENKSVRSLCKGADGVIYVGSTGEF
                     GYLQPTANGRLQYFSLIKNLPDSIQSSLSYIYKTYWYEGKTYFCSLSNIFIFDGNTIK
                     PISLGKQSEYANFFTLKANDKFYVNSYLQGLRTFTSDSTLEVIPGGQAFIHKNVFSIV
                     AIDSSKIFIFTDNGFYTYSNGKVESVDTPTLLAKRMATESAIPYSATKLNDGNIALGV
                     VQSDFFGVVTLAPDLKTINLVNKQTGMHAGQVSEVFQNGDAPLWATLYDGGIAKVELN
                     SAIGRFGQECGLSEIIVDIVRFNNTIYLATFNGVYYLAYDSNGVPKFIPVDGINGNVW
                     ALTIFSPPNAPKMLLAGSYTDGVFEIKGSKAISISNPLNANLELSKQIQHSCFALYPS
                     KSIPGRLYIGLNSGVAYIDWANGEWKHTDNLFRDSINFEIRSIVEDSKGNLWLATGYS
                     GVYMVNKSFTKLSKFGKESIKGLENLQYIILFPVNDSLYILTSNGIYHFNYENNLFEP
                     GGLVGSAFSKRFGIYKATKFSNGYAFLCYDSNNKNWIERVVKDSAGNWVTDNRDLKRL
                     PNKWADAIYADPDGTLWIGMSKELYVYNPHLKRSFNAPFRAVIREVVSKDSLLFGGAF
                     YTQGDSSVRKLSLEQQPYQMPRLGYHYNAMVFTFAAPYFEREEDIVYSHYLEGSDETT
                     WSQWDKRTDATYTNLREGKYTFHVKAKNIYGDESIEATYSFEIRPPWYRTILAFILYF
                     ILAVCLVWGIVKWNTRRLIAEKERLEQIVRERTAEVVAQKEEIEQQKEKIAAQNEEIT
                     SSIQYASRIQTALLTPTDQINRIFPENFILYLPRDIVSGDFYYITQAGKLKVSVVADC
                     TGHGVPGGFMSMLGISFLTQIIGTKTDLKANEVLNELRNLVITALHQTGEIGGSKDGM
                     DIAIYIIDEETQTLQFAGANNPLILIRNNELTHIKGDKMPIGIHLRGELSFTNNVIQI
                     QKGDVIYTFSDGYVDQFGGEDGRKFMIKHFKELLLEIHHKPMSEQRQILDETLKNWHG
                     NTPRIDDVVVMGVRIV"
     gene            complement(3379..5901)
                     /locus_tag="PK049_10480"
     CDS             complement(3379..5901)
                     /locus_tag="PK049_10480"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOK62026.1"
                     /translation="MLQKIQNRNYWIKPKVIGGFLLIIIFSVTAIVMTYQAVSDLKTT
                     RQGLGLPGQKITVLNRLLASIYTEDSDFRMYVLTSNDDYLKTYNNNHQKVTAAIKSLQ
                     KLAYSNQSQLNSIKKIEKLLQNKREIEGEILQMRQSINTSIFYDRAMQRIARAGSETQ
                     RYHEVYKQKTVTQVRRDTVIYKKVDNESMAARIKRFFLGPERIDTIATDTLVEFTYDT
                     IPHITYISDSIIQRLLLILDNIRNDQQNQLRLIDEKEKELLNRNRAILNQVQLIVTSI
                     EKQEQAEAVSQALSVQEVLTKNLLIIFLLGTITFIVLVILIAIIMRDISRNSTYNAQL
                     IEAKQYAENLLRLKEQFLANMSHEIRSPLSAIVGVSRQLSKTELNGKQKEYVDVLNSS
                     SNHLMAVINDILDYSKLATGKLRLESREFSPREVVNDTVKLFEARANEKGLQILSNID
                     QSLPHIVVGDDFRLRQVLINLLSNAIKFTEQGSIIVSGAVLRKTDDSLKIQFTVADTG
                     IGISPEMQSQVFEEFTQADGGISRKFGGTGLGLTIVKKLVELQGGEISLTSEPNEGTI
                     IRVVIPYSIKETRTANVDQETHYRIKAGTSILIIDDDEVNRLIVTEIAKNVGLEVDAI
                     SDAKNLTDQLKAKKYDAVITDIQMPEVSGYEVVQLIENAGYSIPVFAITANDMIDNPE
                     RFSSLGFSGYLIKPFTEADLIRLLGPVIGATGVVTGDKRERADTYNKFDIEEIYRFTG
                     GNKESVKLILESLLENTRKNVEELNQLIKSKNLTKASAVAHKMKSAFNQFRVYDIASI
                     LQRIETLPPDKHKAATLYMDRLNRRVKSFTVELQEQIDNLLN"
     gene            complement(6028..7152)
                     /locus_tag="PK049_10485"
     CDS             complement(6028..7152)
                     /locus_tag="PK049_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK62027.1"
                     /translation="MKKILFALILLASQTVLAQETVTIKFIRPSKVQGSAAKIRIIIN
                     DNEYVLKNGTETTVIIPHDYSKPLLVEASAGGTRQSYRLRAIPSEKYVFETGFEFKGI
                     YLKLLEGKELAVGEVVHKVDTAKAGGETTLKVDKDGRAVGFTIERIDESEAIRQEWLS
                     RGGRIKNESVLLTGTYFGMNLKDFDTRVDGYGGGISAYQNWINLKIPEFKSGISTWNS
                     FNYGWGIDMLLYRYKLNMDLSPSGSMDMSVLTLSYPISANLGWTFGIGKFLDQGNWKG
                     VALTLKYKPSLTLNMVSTTSTIKMPAPLNTTQTDYSSDTNASINLGGFGVDFEFSNFT
                     ATMNRLAPTPKTKFSFFVLPPVGDTPLFVSISLGISWYSR"
BASE COUNT         2105 a         1492 c         1549 g         2414 t
ORIGIN      
        1 gttaaaaata ttgactgatt aaacgttttt ttaatatatt tatcaaatct tagtatagcg
       61 ttttaccatg aaaaagcttc tatttcttgg tattttgttc ataagtgttt tacaagcgag
      121 tggccagcaa ttcaataagt atggcaatcc gcttattaca aactataccc ctgaggttta
      181 cggtggaaac gagcaggttt ggtgtatcgc acaggacgat aggggtgtga tgtactttgg
      241 tacaaacgat aatggcattc tggaatacga tggcaaaacc tggcgtagca tccctatgcc
      301 tgaaaacaag tcggttcgtt cgctttgtaa gggtgccgat ggggttatat atgttggttc
      361 aacaggtgag tttggctacc ttcagcctac tgcaaacggg aggttacaat atttttcact
      421 tatcaaaaac ctacccgata gtattcagtc tagcctttct tacatttaca aaacatactg
      481 gtatgaaggt aagacttact tttgctctct ttctaatatt tttattttcg atggtaatac
      541 tataaaacct atttcattgg gaaagcaaag tgaatatgct aactttttta cattaaaggc
      601 taacgataag ttttatgtta acagttatct tcaaggttta aggactttta caagcgacag
      661 tacccttgaa gttatccccg gtggtcaggc ttttattcat aaaaatgtat tctcaattgt
      721 agcaattgac tctagcaaaa tttttatttt tactgataat gggttttata cctattcaaa
      781 cggtaaggtc gaaagcgttg acactccaac gctccttgcc aaaagaatgg ccaccgagtc
      841 agcaatacca tacagcgcaa ctaaacttaa tgatggcaat attgctttgg gggttgttca
      901 atccgacttt tttggagttg ttacgctagc cccggactta aaaaccataa atctggttaa
      961 caaacaaacg ggaatgcacg caggccaagt gtccgaagtg tttcaaaatg gcgatgcacc
     1021 cctttgggca acactatacg atggtggtat tgccaaggtt gagttgaact ctgccatagg
     1081 aagatttggt caggagtgtg gcttatcgga aattattgtt gacattgtta gatttaataa
     1141 tactatttat cttgctactt tcaacggggt ttattatttg gcttacgaca gcaatggagt
     1201 tccaaagttt atacctgtag atggtattaa tggaaatgta tgggcgttaa ctattttttc
     1261 accaccaaat gcacccaaaa tgcttcttgc aggcagctat accgatggtg tttttgaaat
     1321 aaagggaagt aaagccataa gtattagcaa tcctttaaat gcaaaccttg aattatcaaa
     1381 acaaattcaa catagttgtt ttgctttata cccatctaaa agcatacctg gtagattgta
     1441 tattgggtta aattcaggtg ttgcatatat agactgggca aacggcgaat ggaaacacac
     1501 tgataactta tttcgcgatt ccattaattt tgaaatacgc tcaattgttg aagacagtaa
     1561 agggaacctt tggcttgcca caggttactc tggtgtgtat atggttaaca agagttttac
     1621 caaattatct aagtttggca aggagagcat aaaggggcta gaaaaccttc agtatataat
     1681 tcttttccct gttaatgatt cactatatat ccttacttca aatggaatat accactttaa
     1741 ctatgaaaac aacctgtttg agccgggagg tttagtaggt agcgcttttt caaagcgatt
     1801 tgggatttat aaagccacaa agttctccaa tggttatgcc ttcctatgct acgatagtaa
     1861 caataagaac tggattgaac gagttgttaa ggattctgct gggaactggg taactgacaa
     1921 ccgcgatcta aagcgacttc ccaataagtg ggccgatgcg atatatgctg atcctgacgg
     1981 aaccctttgg attggcatgt caaaagaact ttacgtttac aatcctcatt taaaacgaag
     2041 ttttaacgct ccattccgtg ccgtaattcg cgaagtggta tcaaaggatt cattactgtt
     2101 cggtggagcc ttttatactc aaggggattc ctcggttcga aaattatcgt tagagcaaca
     2161 accttaccaa atgccgcgat tgggttacca ctataatgcc atggtgttta cttttgcagc
     2221 tccttacttt gaacgtgagg aggatatagt gtatagccat tacctggaag gttctgacga
     2281 aaccacttgg agccagtggg ataaacgaac cgatgctact tatactaatc ttagggaggg
     2341 taaatataca ttccatgtta aggcaaagaa tatttatggg gatgaaagca tagaggcaac
     2401 ctactctttt gaaattcgcc cgccctggta ccgtactatt ttggcattca tactctattt
     2461 tatacttgca gtttgtttgg tttggggtat agttaaatgg aatacccgcc gactcattgc
     2521 tgaaaaagaa cgtcttgaac aaattgttcg cgaacgaacg gccgaggttg ttgctcaaaa
     2581 ggaagaaata gaacagcaaa aggaaaaaat tgctgcccag aacgaggaga taaccagtag
     2641 tattcagtat gcaagccgca ttcagacagc attacttaca cccaccgatc agattaaccg
     2701 aatctttcct gaaaacttta tactttactt accccgtgac atagttagcg gcgatttcta
     2761 ctacatcact caggcaggca agttaaaggt ttcagtggtt gccgattgta ccggacatgg
     2821 agttcccggg ggcttcatga gcatgttggg catatccttc ctaacccaga tcattggtac
     2881 aaaaaccgat ctgaaagcaa atgaagtgct taatgagtta cgaaacttgg ttataactgc
     2941 acttcaccaa accggtgaaa taggcggaag caaggatggg atggatattg ccatttacat
     3001 tattgatgag gaaacccaaa cccttcagtt tgccggggca aacaatccgc ttattctgat
     3061 ccgcaacaac gagttgaccc atattaaggg cgataaaatg cctattggca ttcacctgcg
     3121 cggtgaactt tcgtttacca ataatgttat tcaaatccag aaaggggatg taatttatac
     3181 tttctccgac ggatatgtag accagtttgg aggcgaagat ggccgtaagt ttatgataaa
     3241 acactttaaa gaacttctcc ttgaaatcca tcataaaccc atgagtgaac agcgccaaat
     3301 ccttgacgaa actcttaaga actggcatgg taatacccca agaatcgacg atgtagttgt
     3361 tatgggagtg cggattgtct agttcaataa gttgtcaatt tgctcctgca gctcaactgt
     3421 aaaactcttt actcttcggt ttagcctatc catgtatagt gtggctgcct tgtgtttgtc
     3481 gggggggagc gtttcaattc gttgaagtat acttgctatg tcgtaaaccc taaactggtt
     3541 aaaagccgat ttcatcttat gggctaccgc tgaagcctta gttaaattct tgcttttaat
     3601 gagctgattt agctcttcaa cattttttcg ggtgttttca agtaacgact ctaaaattag
     3661 cttaacgctt tctttatttc ccccggtaaa tcggtatatc tcctcgatat cgaatttatt
     3721 gtaagtatca gccctctctc ttttatcgcc cgtaacaact ccagttgctc caattacagg
     3781 gccaagcagt cgaatgaggt ctgcttcagt aaatggcttt atgaggtatc cggagaaacc
     3841 caaactgcta aaacgttcag gattgtcaat catatcgttt gcagtaattg caaaaactgg
     3901 aatgctataa ccggcatttt caattagttg aaccacctcg tagcctgaaa cttcgggcat
     3961 ttgaatatcg gttataaccg catcgtactt ttttgcttta agctgatcag ttaagttttt
     4021 ggcatcgctt atggcatcaa cctctaggcc aacgtttttg gcaatttcag taactattaa
     4081 ccggttaacc tcatcgtcgt caatgattaa gattgaagtg ccagctttta ttctgtaatg
     4141 ggtttcttgg tcaacattgg cagttctggt ttccttgata ctgtaaggta ttaccaccct
     4201 tataatggta ccttcattgg gttcgctggt aagcgatatt tcaccaccct gtagctcaac
     4261 tagtttcttt acaatggtta accctagccc tgttccacca aacttgcgtg atattccgcc
     4321 atcggcctgg gtgaattcct cgaatacttg cgactgcatc tcaggagaga tgccaatacc
     4381 ggtatcggca acagtaaatt gaatttttaa cgaatcgtcg gttttgcgga gtactgctcc
     4441 cgatactata atgctgccct gttcagtgaa cttaatggca ttacttagca ggttgataag
     4501 tacctgtctt aacctaaagt catcgccaac aacaatgtga ggtaacgatt gatcgatatt
     4561 cgaaagaatt tgtaaccctt tttcatttgc tcttgcctca aaaagtttaa ccgtatcgtt
     4621 taccacttcg cgtggcgaga actctctgct ttctaatcgt agtttgccag tggctagttt
     4681 tgagtagtcc agtatatcgt taattacagc catcaggtgg ttcgacgagc tattaagcac
     4741 atcaacgtac tccttttgct tgccattcag ttcagttttt gatagttgac gcgaaactcc
     4801 tacaattgca cttaatgggg aacgaatctc gtgactcata tttgccagaa attgctcttt
     4861 caaacgcaaa agattttcag catactgttt tgcctcaata agttgagcat tgtatgtact
     4921 gttgcgcgat atatccctca ttataattgc tataagtata accagcacaa taaacgtaat
     4981 ggtcccaagc aggaatatta ttaggagatt tttggttaag acttcttgta ccgataatgc
     5041 ttggctaaca gcttcagcct gttcctgttt ctcaatactg gttacaataa gctgaacctg
     5101 attcaggatg gctctattcc tgttaagcag ttctttctcc ttttcgtcga taagcctaag
     5161 ttggttctgc tgatcattcc ttatattgtc cagaattagt aaaagccgtt gaatgattga
     5221 gtccgatatg taggtgatgt gaggtatggt atcgtacgta aattcaacaa gggtatcggt
     5281 agcaatagtg tcaattcttt ctggtcccag gaagaatcgt tttatcctgg cggccatcga
     5341 ttcattgtct acctttttgt aaattacggt atcgcgtctt acctgggtta ctgttttttg
     5401 cttgtaaacc tcatggtaac gctgggtttc cgaccctgct cgggcaattc gttgcattgc
     5461 cctgtcgtaa aagatgctgg tattaatact ttgtcgcatt tgtagtattt cgccctcaat
     5521 ttcccttttg ttctgaagta gcttttcaat cttttttatt gagtttagct gcgattggtt
     5581 actgtatgcc agtttttgaa gcgattttat tgcagcagta actttttggt gattgttgtt
     5641 gtaggtttta aggtaatcgt cgtttgaggt cagtacatac atcctaaaat cgctatcctc
     5701 agtataaatt gatgcaagca acctgttcag aacagtaatt ttttgtccgg gaagcccaag
     5761 cccttggcgg gtagttttta gatcgctaac cgcctggtag gtcataacaa ttgctgtaac
     5821 ggaaaaaata attatcagta agaaaccacc aattaccttt ggcttaatcc agtaatttct
     5881 gttttgtatt ttttgaagca ttgaggttgt aattagcaac caaagttaaa gaaaattatt
     5941 cccttttttg actatacctt tgacttttag ggtgtaataa aaaaagaccg aacatttgtt
     6001 cggtctttat tatacttttt actcaaacta ccttgagtac cacgagatac ctaaacttat
     6061 tgatacaaat agcggtgtgt cgccaactgg tggtaataca aagaatgaga acttagtttt
     6121 tggggtgggg gctaacctat tcattgttgc ggtgaagttt gaaaattcaa agtcaacgcc
     6181 aaaaccgcct aggttgatgc ttgcatttgt atcgctgctg taatcggttt gggtggtgtt
     6241 tagtggtgct ggcattttta tggttgaggt agtgcttacc atgtttagcg ttaatgaagg
     6301 tttatactta agggttagtg ctaccccttt ccagtttcct tggtcaagaa acttgcctat
     6361 cccaaaagtc caaccaaggt ttgcagagat gggatacgat agggtaagaa ccgacatatc
     6421 catacttccc gatggggaaa ggtccatatt taatttgtac ctatacagca gcatgtcaat
     6481 gccccatccg tagttgaaac tgttccaggt tgaaattccc gatttgaact ctggaatttt
     6541 aaggttaatc cagttctggt aggccgatat acccccaccg taaccatcaa cgcgggtgtc
     6601 aaaatccttc aggttcattc cgaagtaagt tccagttaaa aggactgact cgtttttaat
     6661 tcttccaccc cgtgaaagcc actcctgcct tatggcctcc gactcatcaa tccgctcaat
     6721 ggtaaatccc actgctcgcc cgtctttatc aacttttaga gtggtttcac cccctgcttt
     6781 tgcagtatct actttatgta ccacttctcc tactgcaagc tctttcccct caaggagttt
     6841 caggtaaatc cctttgaact caaaacctgt ttcaaacaca tacttctcgc taggaatagc
     6901 tctcaatctg tacgattgac gagtaccacc agccgatgct tcaactagta gaggtttgct
     6961 gtaatcgtga ggaataatta ccgtggtttc ggttccattt ttaagaacat attcgttatc
     7021 attaatgata attcggattt ttgcagctga cccttgaacc tttgatggtc taataaattt
     7081 tatggttaca gtttcctgtg caagtactgt ttggcttgcg agcaaaataa gtgcaaatag
     7141 aatttttttc atggcctgtg gttggttttg tttggttggt caagttaatg catattctat
     7201 gttttgtcaa tctgttgggt ctggaatttg ataagatcac tttttggttt gatagtacag
     7261 aaataaatga atatcaacag ctaatattta tagacaaagg ctttaattaa gaaactcttt
     7321 tattggtgaa ctaagccata gcattaaatc aaattgagtt ccccttttgt ttagtttcat
     7381 cctattaaca gctgggtgca gagctagtcc cgccaagcga gaactcgaac ctgcgagtga
     7441 aaaaatcaac aggagattat gcgttaaaaa attggattta gcagttaagt gaggattaaa
     7501 aatacaaaac cccgccattg gcggggctta cttctttgta gcgggggcag agccagtccc
//
LOCUS       DAOSAL010000097         7317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_6523_length_7317_cov_49.956073, whole genome shotgun sequence.
ACCESSION   DAOSAL010000097 DAOSAL010000000
VERSION     DAOSAL010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 7317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7317
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6523_length_7317_cov_49.956073"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(252..2030)
                     /locus_tag="PK049_10490"
     CDS             complement(252..2030)
                     /locus_tag="PK049_10490"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOK62028.1"
                     /translation="MSERKLLKSGEFLVNEIDANDIFIPEEFNEEQRMIAQTCRDFME
                     AEVFPRLNDIDKGDRELMRSILQKSGELGMLGIAVPEQYNGFGQNFVTQMLVAETTGA
                     GYSFSVAYMCHCGIGTMPILYYGNEEQRQKYVSRLATGELIGSYCLTEPGAGSDANSG
                     KTTAKLSEDGKYYILNGQKMWITNAGFADTQVVFAKVDNDRVLSAFIVESKWPGVVIG
                     PDEHKMGIKGSSTAQIYYNDVKVPVENMLGNRGEGFRIALSILHMGRMKLGANVIGAA
                     KETITQSVQYANERKQFNTKIANFGAIKHKLAEMVIRAYAHESAIYRVSQDIDDLIEK
                     YKAEGCEYGRAAIDAISHYAVEDAILKVNGSEMLDFVVDEGVQIHGGMGYSAEMNVER
                     GYRDSRINRIFEGTNEINRLLVIDTAIKRSLKGDYDLMGPAEQLYNSLDSIVAEAGSS
                     DYYDQKMQTIRNFKKVAMLGIYGANKAFGKQFATEQEVQNNLSNIIMDLYVAESLALR
                     VKKLESKGHQNINIYKDIVDVFVYDAAGRIRKNAMDAANSFAAEPELSKLTAAIDRLC
                     KAPSVNVKDARRRIADKLIEDNEYKF"
     gene            complement(2055..2849)
                     /locus_tag="PK049_10495"
     CDS             complement(2055..2849)
                     /locus_tag="PK049_10495"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOK62029.1"
                     /translation="METRKIEIPVMADLFARGEKPEYLFWVGCAGAYDDRYKKVARAF
                     AKILVHLNVSFAVLGKEETCTGDPARRAGNEMLFQMQALQNIELFKAYEVKKVITICP
                     HCYNTFKNEYPDLGADFEVISYIDILNKYIDEGKLSIDTDKFKSAKITYHDPCYLGRA
                     NGIYEQPRAILKKLGGELVEMERNRSFALCCGAGGAQMFKEAEHGNKEVFLERMDDVL
                     KVDPNIIATACPFCMTMMTDGIKYKNQEEKMRNLDIAELIAESLNL"
     gene            complement(2894..4213)
                     /locus_tag="PK049_10500"
     CDS             complement(2894..4213)
                     /locus_tag="PK049_10500"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOK62030.1"
                     /translation="MTKQIVFAIALLVTIGVFAYTISRIVRFFMLTRKGFAVSKLGKR
                     LWVTLEVAFGQTKIFRRPVIGLMHALVFWGFCVILFGSIEMVIDGLFGVEKSLSVLGA
                     FYNFMMATGDIFALLVAISIVVFLIRRVFLHIKRFEGIEMKKISHIDANVALSIILVL
                     MLSLMGMNAAYLKWVELAGHEVHGVYPVSQHLTWLFNGMSVDTVFTHYQIYWWTHILL
                     IFLFANLLPYSKHFHVFMSVPNVFLSRLEPLGKLPNMDNVTREVKLMMDPNAAFAAPA
                     NDAPIERFGVKDAEDVVWKSYFDALSCTECGRCTSVCPANITGKKLSPRKVMMDLRAR
                     MKEKGPMMLKNGRDYTDGKSLVRDYISEEELWACTTCNACAQECPINIDHPKLIVDMR
                     RYLVMEEGSAPGELKAMFNNIENNGAPWQYSPEDRLVWAKDLEINVQ"
     gene            complement(4342..5307)
                     /locus_tag="PK049_10505"
     CDS             complement(4342..5307)
                     /locus_tag="PK049_10505"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOK62031.1"
                     /translation="MSVLVYTENWDGKFKKLSFELVSYAGKLAEMMGTTTTAVTIGEV
                     SNDELKQLGKYGADKVICLKSDNLKNLDSQLYTSAIAQVAQSEGSKVIVIANNNSGKA
                     IAPRLSVRLKAALGSGVSQLPLSLSPFTVYKRAFSGGAFAQVVLKTDIKIIALTQNSF
                     DIVENSKDVKIERVDIQVDAPKTVVKDVQKQTGKLLLTDAEIVVSGGRGMKSPDNWGP
                     IVELAELLGGATACSRPVSDEGWRPHDEHTGQTGKIIAPNLYIAVGISGATQHIAGVS
                     GSKYIVAINNDKSAPIFEVAQYGIVGDAQKVLPKLVEAVKEIKGK"
     gene            complement(5333..6082)
                     /locus_tag="PK049_10510"
     CDS             complement(5333..6082)
                     /locus_tag="PK049_10510"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOK62032.1"
                     /translation="MKILVCISHVPDTTTKVKFVDGDTKLDTNGIQWVINPWDELSLT
                     RALELKDDASTGVKSVTVAHVGNATTEPTIRKALAIGADDAIRVNAEPTDAYYAAAQL
                     AEVVKKEQFDIVMCGIESSDYNNSAVGSMLAEFLGIPSVSAVSNIKVENGQAIITREI
                     DGGKEVLTVPTPFVAIVQKGIAKEPRIAAMRGIMMARTKPIKLYEPVAVDALTEYVKF
                     EMPKPRAACKFVDPENPKELITLLQNETKLI"
     gene            6485..6964
                     /locus_tag="PK049_10515"
     CDS             6485..6964
                     /locus_tag="PK049_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK62033.1"
                     /translation="MSNEVIHSLIRNLDEKLSYSLVQLEKVSQEVTVHLQNNPEHSSD
                     ELVPALIRHFDIRRQSISYLKLMLKLRSFLNNDSKYILNFEKSSGIRIDCISEQMIIN
                     EKMPTYLSNNLNVDALNMEVYTSKAYQRYFEETYNELMRADRFEACSILETMKFSYN"
BASE COUNT         2052 a         1698 c         1476 g         2091 t
ORIGIN      
        1 gccttgatct tctttggttc tttcttgtat caagacaaga aagaacatgt aaaccgctct
       61 ttgcggtgct tttatgttgc gatgcaatga attgaaatgt ggctatggaa aaaattttga
      121 ggggattagc caatagaagc acataaaata ctcttcactt ctgacttatc gaggcaatcc
      181 cgattaaacg ataaagttaa aaaaagaaag aagctacccc ttgggtagct tcttaacttt
      241 tactaagtct actagaactt atactcgttg tcttcaataa gtttatcggc aatgcggcgg
      301 cgggcatcct ttacatttac cgatggagcc ttacaaagcc tgtcaattgc agcagttaac
      361 ttactaagtt cgggctcagc agcaaaggag tttgctgcat ccatggcatt cttacgaatg
      421 cggccagcag catcgtaaac aaacacatca acaatatcct tatagatatt gatattttga
      481 tgacccttac tttccaactt cttaaccctt agggcaagcg actcggccac atacagatcc
      541 atgatgatat tgctcaggtt gttctgaacc tcctgctcag ttgcaaactg cttaccaaaa
      601 gccttgtttg ctccataaat acccagcatg gcaacctttt tgaagttcct tatggtctgc
      661 attttttggt catagtaatc gctcgaaccc gcttcagcca caatgctatc gaggctattg
      721 tatagctgct cagcaggccc catcaaatcg taatcgccct tgagggagcg cttaatggcg
      781 gtatcaatta ccagcaagcg gttgatctca ttggtaccct caaagattcg gttaatacgc
      841 gaatcgcggt agccgcgctc aacgttcatc tcagctgagt agcccattcc gccgtgaatc
      901 tgaacaccct catcaaccac aaagtcgagc atctcggaac cgtttacctt cagaatggca
      961 tcctcaacgg cgtagtggct aatggcatca atggcagcac ggccatactc acaaccctca
     1021 gccttatact tttcaatcag gtcatcaata tcttggctta cacggtaaat ggccgattcg
     1081 tgagcatagg ctctaattac catttcagct aacttatgct tgatagcccc aaagtttgca
     1141 attttggtat tgaactgctt acgttcattg gcgtactgaa ccgactgggt tatggtttct
     1201 ttagcagcac caatcacatt ggcacccaac ttcatacgtc ccatgtgcaa aatgctaagg
     1261 gcaatacgga aaccttcgcc gcggttacct agcatatttt caacaggcac ctttacatcg
     1321 ttgtagtaga tttgagcggt tgatgagccc ttgataccca ttttatgctc atcggggcca
     1381 attacaacgc ctggccactt gctttcgact ataaatgcac tcagcactcg gtcgttatca
     1441 accttggcaa aaacaacctg tgtatcggca aagccggcat tggtaatcca cattttttga
     1501 ccattcagga tgtagtactt accatcttcc gatagctttg ctgtggtttt tccggagttg
     1561 gcatcgctac ctgcaccggg ctctgttagg cagtatgaac caatgagttc accagttgca
     1621 agacgcgata catatttttg gcgctgctcc tcgttgccgt agtaaagaat gggcattgta
     1681 ccaattccgc aatggcacat gtaggccacg gaaaacgaat atccagcccc tgtagtttcg
     1741 gcaacaagca tttgggttac aaagttttgc ccaaaaccat tatactgctc aggcactgca
     1801 atgcccagca taccaagctc gcccgatttt tgcaaaatgc tcctcatcag ctcacggtcg
     1861 cccttgtcga tatcgttcag gcgagggaaa acttcagcct ccatgaaatc gcgacaggtt
     1921 tgggctatca tccgctgttc ctcattgaat tcctcgggaa taaaaatatc gttagcgtca
     1981 atctcattta caagaaattc gccacttttt agcaattttc tttcgctcat agctgttttt
     2041 ggtttattga ttagttatag atttagcgat tctgcaataa gttcagcaat atcgaggttt
     2101 cgcatcttct cttcctgatt cttatacttg ataccatcgg tcatcatggt catgcagaag
     2161 gggcaagccg ttgcaatgat gttggggtca acctttaaaa catcatccat acgctccagg
     2221 aaaacctcct tgttaccgtg ttcggcctcc ttaaacattt gagcaccacc agcgccacag
     2281 cacagggcaa aactcctgtt gcgctccatc tctactagtt cgccacccaa ctttttaagt
     2341 atggcacgtg gctgctcgta aataccattg gcacgtccta aataacaagg atcgtggtat
     2401 gtgattttgg cactcttgaa tttatcggta tcgatactca atttgccctc gtcaatatac
     2461 ttattcagaa tatcgatata gctgataacc tcaaagtcgg cacccaaatc ggggtattca
     2521 ttcttaaagg tattatagca gtgggggcaa attgttatca ccttctttac ttcgtatgcc
     2581 ttaaaaagtt caatattctg taaggcctgc atctgaaata gcatctcgtt tccagcccta
     2641 cgggcaggat cgcccgtgca ggtctcctcc ttgcccaata cggcaaagct aacgtttagg
     2701 tgaaccagta ttttggcaaa agcacgagcc actttcttat agcggtcatc gtaagcgccg
     2761 gcacagccaa cccaaaaaag gtattcgggc ttttcgcctc gggcaaagag gtcggccata
     2821 accgggattt ctattttgcg agtttccatt ttccaattag tttaagttga tactgatagc
     2881 agggaacgca atgctactgt acgttaatct caagatcttt tgcccaaacc aagcgatcct
     2941 caggcgaata ctgccaggga gcaccattgt tctcaatatt gttgaacatg gccttgagct
     3001 caccgggtgc cgacccttcc tccatcacca ggtacctgcg catatcaaca atgagtttgg
     3061 gatgatcaat gtttatgggg cactcctgtg cgcatgcatt gcatgtggta catgcccata
     3121 gctcctcctc gctaatgtaa tcgcgtacaa gcgatttccc atcagtgtaa tcgcgcccat
     3181 tttttagcat cataggacct ttctccttca ttcgggcacg caggtccatc atcaccttgc
     3241 ggggcgatag tttctttcct gtaatgttag cagggcatac cgatgtacag cgcccacact
     3301 cggtgcacga taaggcatca aaatagcttt tccatacaac atcctcagca tctttcacac
     3361 caaatcgctc aattggagca tcgtttgcag gtgctgcaaa tgcagcattg ggatccatca
     3421 tcagctttac ctcacgggtt acattatcca tgttgggcaa tttgccaagc ggctcaagcc
     3481 gcgagaggaa tacgttgggt acagacatga atacatggaa atgcttggaa taaggcaata
     3541 ggtttgcaaa caggaagatt aacagaatat gtgtccacca gtaaatctgg taatgggtga
     3601 atacagtatc aactgacatc ccattgaata accaggtgag atgttggcta acggggtaaa
     3661 ccccatgcac ttcatgccct gccagctcaa cccacttaag gtatgcagca ttcatcccca
     3721 ttagcgagag catgagaacc aggatgattg aaagagccac attcgcatca atatgtgaaa
     3781 tcttcttcat ttcaatgccc tcaaatcgct tgatatgaag aaataccctt cggataagga
     3841 ataccactat tgaaatggca accagaaggg caaaaatatc gccggtagcc atcatgaaat
     3901 tgtagaatgc gccaagaaca cttagcgact tttcaacgcc aaataacccg tcgattacca
     3961 tttcgatact gccaaagagg atcacacaaa agccccagaa cacaagggca tgcattaatc
     4021 caataactgg cctgcgaaaa attttggttt gcccaaaagc tacctcaagg gtaacccata
     4081 gccttttacc cagcttactt accgcaaatc ccttacgtgt aagcatgaaa aaccttacaa
     4141 ttcgggatat ggtgtaagca aaaacaccga tggtaaccag cagggctatg gcaaatacta
     4201 tttgtttggt catgtttttg gtttttggtt tacaatggtt tgttggtaaa aacaaacatc
     4261 tggagtaagt gcctctattt tgcctaagaa cctgctgcaa acgcaacagg ttcatgtctt
     4321 atgatttgta agaatggtga actactttcc ttttatctct ttaacagcct caaccagctt
     4381 tggtaaaacc ttttgggcat cgcctacaat accgtactgg gctacctcaa aaattggtgc
     4441 tgatttatcg ttgttaatgg caactatgta cttggaaccg ctaacacctg caatatgctg
     4501 tgttgctcct gaaataccaa ctgcaatgta aaggttagga gcaataattt taccggtttg
     4561 tccggtgtgc tcatcgtgtg ggcgccatcc ttcatcggaa actgggcgcg aacatgctgt
     4621 ggctccgcca agcaattcgg caagctcaac tattggcccc cagttatcgg gcgatttcat
     4681 tccgcggcca cccgatacaa caatttcggc atcggttaaa agcagcttac cggtttgctt
     4741 ctgaacatcc ttaaccacag tttttggtgc gtcaacctga atatcaaccc tctcaatctt
     4801 tacatctttg ctgttctcaa caatatcgaa agagttttgt gtaagagcaa taattttgat
     4861 atcggtttta agcactacct gtgcaaaagc accacctgaa aaagcgcgtt tgtaaacggt
     4921 aaatggcgat aggcttaggg gaagttgcga tactcccgaa cccaatgcag ctttaagcct
     4981 taccgataaa cggggtgcaa tggccttacc gctgttattg ttagcaatta caatcacctt
     5041 gctaccttcc gactgagcaa cctgagcaat agcgctagtg tatagctggc tatcgaggtt
     5101 tttcaggtta tcgctcttta ggcaaataac cttatcggcc ccatacttac caagttgttt
     5161 gagctcatcg ttggacacct caccaatggt aactgcagtg gtggtagtcc ccatcatttc
     5221 ggctaacttg ccggcgtacg aaacaagttc gaatgataat tttttgaatt taccgtccca
     5281 attctcggta tatactagta ctgacatgtt ttacaaattt tttggttaaa ccttaaatga
     5341 gtttggtctc gttttggagt agcgtaataa gttctttggg attttcggga tccacaaact
     5401 tacatgccgc cctgggtttg ggcatttcaa acttaacata ctccgttagc gcatcaactg
     5461 caacaggttc atacagtttg atgggctttg tacgagccat cattataccg cgcatggcag
     5521 caatacgggg ttccttggca atacccttct gcactattgc aacaaaagga gttggcacgg
     5581 taagaacttc cttaccacca tctatttcac gggttatgat tgcttgacca ttctcaacct
     5641 ttatattcga tacagctgaa accgatggaa taccgaggaa ttcagcaagc atgctaccaa
     5701 ccgcagagtt gttatagtcg ctcgattcaa tcccgcacat aacaatatcg aattgttcct
     5761 ttttaaccac ctcagccagt tgggccgcag catagtatgc atcggtaggt tcagcattta
     5821 cacggatggc atcgtcggca ccaattgcca aagccttacg aatggtaggc tcagtagtgg
     5881 cattgccaac atgtgcaacg gtaacgcttt ttacgccagt actggcatca tcctttaact
     5941 ccaaagcccg ggttaacgat agttcatccc aggggttaat cacccactgt atcccattag
     6001 tgtcgagctt agtatcgcca tctacaaact tcaccttggt tgtggtatcg ggtacgtggc
     6061 taatacaaac aagaattttc attggtactg attatttata agttaaacat ctatttagca
     6121 atccttttaa ttatttaaac ccacaaattt aaactaatct tggcataaac aagcctctta
     6181 tttttaaaaa cagccatata taattgtcct aatgccagtt acaaaatatt ttattttttt
     6241 aacagttaca cattcaaacg agcgtacaaa tctatatcaa ttccgtttac aaccaataag
     6301 gtttttgctt ttaacaatcg attgtcacac catttatgct tatgattttg actatcaaca
     6361 ttttgcaaat tgataaattt taacagttgt ttagtcattt tttaactaaa gttgaccaaa
     6421 atcaccctaa caaaattgac ttttaaacgt ttatgaaata aatttacatt aaacggattg
     6481 gttaatgagc aacgaggtaa tacatagctt gattcgaaat cttgacgaga agctttcata
     6541 ctcgctggtg cagcttgaga aggtttcgca ggaagttact gttcacctgc aaaacaaccc
     6601 cgagcattca tcggacgagc ttgttccggc gctaattcgc cattttgaca ttaggcgcca
     6661 aagcatttcg tacctcaagc taatgctcaa gttgcgttcc ttcctaaata acgatagtaa
     6721 gtacatctta aactttgaga aaagttctgg cataagaatt gattgtatat cggagcaaat
     6781 gataattaac gagaagatgc ctacttacct tagcaataac ttgaacgtgg atgccttaaa
     6841 catggaggtt tacacatcaa aggcttacca gaggtatttt gaggaaacct acaacgagct
     6901 tatgcgtgcc gataggtttg aggcatgctc catacttgaa acaatgaaat tttcatataa
     6961 ttaggttttg gtttaggttt acaataggtt aggtggttga catccggggc gcttaaaacg
     7021 ttccggatgt cttctttttt aacctaagtg acatgaaaag ttaaaagtga aaagtgaaaa
     7081 gaagaattga gaatataagg gggttagaga gagtttatgt tcgttaaacg tgagccgtga
     7141 gccgtgaaac gtgaaacgtg aaacggaaga attctgagga agttagaggg agttagagga
     7201 agttagagga atatgaaaca ttgagctcca ccaaacacta accactaacc actaaacact
     7261 aaactggttt gaccccctat aaaactggac taaaagtaca aactaaaatt gtacttt
//
LOCUS       DAOSAL010000098         7278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_6565_length_7278_cov_51.685311, whole genome shotgun sequence.
ACCESSION   DAOSAL010000098 DAOSAL010000000
VERSION     DAOSAL010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 7278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7278
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6565_length_7278_cov_51.685311"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..752)
                     /locus_tag="PK049_10520"
     CDS             complement(75..752)
                     /locus_tag="PK049_10520"
                     /inference="COORDINATES: protein motif:HMM:NF036560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas6"
                     /protein_id="HOK62034.1"
                     /translation="MKKLRSLYIGFENPIASSEVVAFRSAIAEKAGRKHILFHNHLDS
                     NEYLYSYPRIQYKVVNQRPAIVCIEEGVDEIHHFFQKPNWDLMLYDRLYNVKVAKLQV
                     NQFTMQVWETPFTYNLYRWHALNQENYTLFRNLTDDNARHELLERILTGNILSFAKGI
                     GWTIDKEVKVKVNAIKKQRWLQLKDKKVLGFDIEFTTNVFLPNYIGLGKNVSIGFGIV
                     KQKKTHK"
     gene            complement(1324..1524)
                     /locus_tag="PK049_10525"
     CDS             complement(1324..1524)
                     /locus_tag="PK049_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK62035.1"
                     /translation="MERLEQFKPFVNPSTGFYYEYLALDELNFFIHYFDFTELLYLGS
                     VDFKFSEKAKMQNLFKLKRFGH"
     gene            1825..3228
                     /locus_tag="PK049_10530"
     CDS             1825..3228
                     /locus_tag="PK049_10530"
                     /inference="COORDINATES: protein motif:HMM:NF017754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOK62036.1"
                     /translation="MNLNKEQKDALAKLIEFVKGNEHDTFILKGYAGTGKTVLVNELL
                     QSPEIKEIPVQLLASTGRAAKILSLKTNRAAQTVHSYIYTFVYDEFNEEKKTIKVQFA
                     LRELNIDLTDTIYIVDEASMLSNHKDNTMEEGLQFGSGKLLTDFFTFCQGRKVIFVGD
                     PAQLPPINTLFSPALNEEYLQNTFKRKVTSTFLTQVMRYSKTSGIYYNTSRIREQLEK
                     PVKPKYLSIIATQFPDMDVVSLDYMLIENYVNTIKKKGYESAILVAYTNKTCNQLNEL
                     VRKKLFTNYQLPQKGDLCIVAKNNYLHGIENGQHIYIQSISEKEERVGQLHFRDIIYY
                     LITPNGIDKSREYCGKVIMEFLLREKPTITHEEEYELYKRFFATIDPEKRKNKQYVID
                     AFSKDPYIHALRIRYGYAITCHKAQGGEWDTLFLILEKVLFGQTKDNPEFLYRWVYTA
                     LTRPVNKVVLLDNICIK"
     gene            3237..4214
                     /gene="cas1"
                     /locus_tag="PK049_10535"
     CDS             3237..4214
                     /gene="cas1"
                     /locus_tag="PK049_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HOK62037.1"
                     /translation="MELVLNTFGTSLQVEDGLFLVVSKDGKQTVDPSTVKSILINRGA
                     KISSDAALLAIANEIDVLFVDATGKPGGRLWSIKYGSISEIRRAQLEFLYSPKAVKWI
                     KNLLRTKIDNQIGLLLSLDAEHENQKVALEQCVNRLRDYQTKIEGVDAPFVNDIAPSL
                     RGWEGAASRAYFATLNLFLPPEYQFKERSQHPATDVFNCLLNYAYGILYGKVEGALIK
                     AGIDPYAGIFHRDDYNRPALVFDVIELYRIWADYVVFSLCCQKVIDQECFSIKDDGSY
                     WLENMGKRILIQSMNDYLDEIIYIKGIARSRLNHIELQAHSLAQTFLKG"
     gene            4220..5245
                     /locus_tag="PK049_10540"
     CDS             4220..5245
                     /locus_tag="PK049_10540"
                     /inference="COORDINATES: protein motif:HMM:NF040562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated primase-polymerase type B"
                     /protein_id="HOK62038.1"
                     /translation="MEPLGVELTRGNSIVSPNDPLRKIDVTDLYQLLVNPPSELESAI
                     NQLRTILSINPKKYQELKRMLPYVTCGMFNPPYRRTANFTSIGCMIVDLDHLSQHGVN
                     AEELKARLKVDPRVLLCFCSPSADGLKLLFKLSQKIYDAARYSMAYKMFITQFANQHG
                     VAHLVDRVTSDVTRATFISIDANAYFNPNAEAVPLHTLINFDSPSSINTAQKQIQEFE
                     FSLPPENTAPKEKNILPSDILQKIKESLNPNIKTKAEKIIYVPQELEPAVEKVKSRMQ
                     ELGISVKQIENIHYGKKFVFELDGRFAQLNLFYGKQGYKVVVTPVRNADAELAEVSRL
                     ILCEIFM"
     gene            5248..5727
                     /gene="cas2"
                     /locus_tag="PK049_10545"
     CDS             5248..5727
                     /gene="cas2"
                     /locus_tag="PK049_10545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOK62039.1"
                     /translation="MPRKKRIPLTLTEKLKKIKTAGLSATLPPNQYDPYQEKIDDLNK
                     RIERVLGIFNKTVKTAEMVYFIMYDIENDKVRNLIAKYLKKNGCVRVQRSVFLTSSDR
                     EKFSEIHQTLKQVQEFYDNTDSIIIVPVTPDSIRTMKLIGQNINIDLALGNTNTLFF"
     gene            5752..7059
                     /locus_tag="PK049_10550"
     CDS             5752..7059
                     /locus_tag="PK049_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK62040.1"
                     /translation="MATIFDYAQQLHNLCVADRYSDALKLYETKIVKQFRQREIDRSF
                     LVINCVTNAYCKTNQWNKGISYGLSKLDCLARMRTDVDYALKTIGWQLLKALIPNPPM
                     VKPETLDFEKVEQLLKMLKERAACSDIALKLYLVWIRMLPGLQVDYLTGQRFYNLFQP
                     EELINWPAEVPEKQLFKPIEVWYLNHSKYLYQHQRYQECCNLCDIGLNQANALSKDCQ
                     AWLTRRYALALKQMGDMNRAITLMEVLARKKLDWFIYHELGELYFQSNDAQNGEKCLV
                     LAYRFGGHNPSKVNLYERLGDHCMNIRMPNIATEFYQLALKVRTEEGWKIPDSLMQKL
                     PTKTTHTDSAQLFRSLLRVFQKTIEVNDMLFGAGEITRILHEGPNGDGFITDTEGNTV
                     YFRFSQANFDSDEVENGYPVKFKARRVLHRGEKRWQATKVYRD"
BASE COUNT         2398 a         1488 c         1462 g         1930 t
ORIGIN      
        1 ttgataaaac ttcccaatat catgcagaag agcacctaaa tatatttgct gacgtatttt
       61 atctttatcg ttattcattt atgcgttttt ttttgtttca caataccaaa acctatgctt
      121 acatttttgc ccaaccctat gtagttaggg aggaaaacgt tggttgtaaa ctcaatatcg
      181 aaaccaagca cctttttatc ctttaattgt aaccaacgtt gtttttttat agcattaacc
      241 ttaaccttta cttctttatc gatggtccaa cctatgccct tggcaaacga aaggatgtta
      301 ccagtaagaa tgcgttcaag cagctcatgc cttgcattat cgtcggttag gttgcggaaa
      361 agcgtatagt tttcctggtt aagggcatgc cagcggtaca ggttataggt gaaaggggtt
      421 tcccatacct gcatggtaaa ctggttgacc tgcaacttgg ccacctttac gttgtaaagc
      481 ctgtcgtata gcatgagatc ccagttgggt ttttgaaaaa agtgatggat ttcatcaacc
      541 ccttcctcta tgcatactat ggcggggcgc tggtttacta ccttgtactg aatgcgggga
      601 taactgtata gatactcatt actgtccaga tggttgtgga atagtatgtg tttcctgccg
      661 gctttctcgg ctattgccga acggaatgcc acaacctctg agctggcaat gggattttca
      721 aaaccaatgt atagcgagcg taactttttc aatgttaggt tattatgagg ttaagtactc
      781 ccacacccaa caatcccaac cgctatccta aattaaataa taaatttaaa attgacaaaa
      841 cttatggtaa gcatttttta ttaacaggtt gttaacaagt ggtctaatgc aattggcatg
      901 tactattttt taaaccctct ttaaaataga actaaagata tgcttgaact aaaagaaatg
      961 gtaacatcaa acaatttatg gacgcatata actaacctgg ttataccttt accctattga
     1021 aaagccttgg ttgtcattta aaaacctgta tgtaccacct tcggtcaact tgataaaaac
     1081 tggttcaata tcaacttcat catccttgag tagggcgagc ttgcaattgg agcaatgggg
     1141 caggaagtta attattccct tagaattatc gaatgttagc aggtagtatg gaaaattacg
     1201 ttgggttaaa cgtttcccca tgtacttaac ctcaagctca caaatcggaa gatcttcatc
     1261 aagtagctgg agaaagtaaa tctgatcttc gggttttgta tttgcccagt aaggttttga
     1321 cgcttagtgg ccaaaacgtt taagcttaaa taggttttgc atttttgctt tctcgctaaa
     1381 cttgaaatca accgacccca agtaaagcaa ctcggtgaaa tcaaagtagt ggataaagaa
     1441 gttcaactca tcgagggcaa gatactcata gtaaaagcct gttgaagggt taacaaatgg
     1501 cttaaactgt tcaagtcttt ccatatcagt tatacaaaat ttgtcggttt cggccaatgc
     1561 ccaatttata tagcctggat caaagagata aaccatccct atctgatagc ctttgtttct
     1621 gccgaatggc attatatcaa gagcattcat aattttttta ataagtaggt aaggttaact
     1681 attacgaata taataaattg aaaaaaatta tatcttcaac ttattatttt aacgaatgcg
     1741 ctaggcaaac tgtgagtaat tatgaattat agcttaacat cctatgaata agcccaaaac
     1801 ataatattct aaaatattat tgcaatgaac ctgaacaaag aacaaaagga tgcccttgca
     1861 aaacttattg aatttgtaaa aggcaatgag cacgatacct ttatactaaa ggggtatgca
     1921 ggaaccggaa aaactgtgtt ggttaatgag ctattacaaa gccctgaaat aaaggagata
     1981 cctgttcaac tgcttgcatc aaccggaaga gcggcaaaaa tcctctcgct caaaaccaac
     2041 cgtgctgctc aaaccgttca cagctacata tacacctttg tttacgacga gtttaacgag
     2101 gagaagaaaa ctataaaagt gcaatttgcg ctaagagaac tcaacattga tttgaccgat
     2161 accatttata tagttgatga ggcatcgatg ttatcgaacc ataaggataa caccatggaa
     2221 gagggattgc agtttggtag cggtaagctg ctaaccgatt tctttacctt ttgccaagga
     2281 cgcaaagtta tttttgtggg cgatccagcg cagctaccac ccattaacac cctattctcg
     2341 cctgcactga atgaggaata cctacagaac acctttaagc gtaaggttac ttcaacgttc
     2401 cttacccagg taatgcgata ctcaaaaaca tcgggcattt actacaacac ttcacgaatt
     2461 agggagcaac ttgaaaaacc tgtaaaacca aaatacttga gcattattgc cacccaattc
     2521 ccggatatgg atgtagtatc gcttgactac atgctaattg agaattacgt aaacacaatt
     2581 aagaagaaag gttacgaaag cgctatactg gttgcttata ccaataaaac ctgtaaccag
     2641 ctgaatgagc tggtgcgtaa aaagcttttt acaaattacc agctcccaca aaaaggcgac
     2701 ctttgcattg tggccaaaaa caactacctc catggtatcg aaaacggtca gcatatctac
     2761 attcaaagca tatcggagaa agaagagcgt gttgggcaat tacactttag ggatataata
     2821 tactacctga taaccccaaa tggtattgat aaatccaggg aatactgcgg gaaggtaata
     2881 atggaatttt tactccgcga aaaacccaca ataacacatg aggaggagta tgagctatat
     2941 aaacgttttt ttgcaaccat tgatccagaa aaaagaaaaa acaaacaata cgtaattgat
     3001 gccttttcaa aagacccata tattcatgca ttaaggatta ggtatggtta tgctattaca
     3061 tgccataagg cacagggggg tgaatgggat actctattcc tgatactaga aaaagtttta
     3121 tttggacaaa ccaaggataa tccagaattc ctataccgtt gggtttacac agcattaacc
     3181 cgaccggtaa acaaggtagt tttactcgat aatatctgca taaaatagat taagccatgg
     3241 aacttgtact taacaccttt ggaacaagcc tacaagttga ggatgggcta ttccttgtgg
     3301 tgagtaagga tggtaagcaa accgttgacc cctccacagt aaaatcgata ctaataaacc
     3361 gtggggcaaa aataagcagc gacgcggcat tgcttgccat agccaatgag attgacgtgc
     3421 tatttgtaga tgccaccggt aaacccggag gccggctgtg gagcattaag tatggatcca
     3481 tttcggagat ccgaagggct caactcgaat ttctttactc tcctaaggct gtaaaatgga
     3541 ttaaaaactt actacgaaca aaaattgata accagattgg tttgttgctc agccttgatg
     3601 cggaacatga aaatcaaaag gttgcattgg aacaatgtgt aaataggtta cgcgattacc
     3661 aaaccaaaat tgaaggagtt gacgccccct ttgtgaacga cattgccccc agcctgaggg
     3721 gttgggaggg agcagcatcg cgtgcttact ttgccaccct taacctgttt ttgccacctg
     3781 aataccaatt caaggaacga agccaacacc cggcaaccga tgtgtttaat tgcctactta
     3841 actatgccta tggcatactg tacggaaagg ttgaaggggc tcttattaaa gcaggaattg
     3901 acccctatgc tggaatattc caccgcgacg actataatcg cccggcatta gtttttgatg
     3961 ttattgaact ttaccgcatt tgggccgatt acgtagtttt tagcctctgt tgccaaaagg
     4021 ttattgacca ggaatgcttc agtattaagg acgatggctc ctactggctc gaaaatatgg
     4081 ggaaacgaat tcttattcag tcaatgaacg actacctgga tgaaattata tatataaaag
     4141 gtatagcccg aagtcgttta aaccatattg aactacaggc tcatagctta gcacaaacat
     4201 ttttaaaagg ataagcaata tggaaccgtt aggagttgag ctaacaaggg ggaacagcat
     4261 tgtaagcccc aacgacccgt tacggaaaat tgatgtaact gacctgtacc agttgctggt
     4321 taacccacca tcggagctgg aatcggcaat taatcagctg cgcaccatac tatcaatcaa
     4381 tcccaagaaa taccaggagc taaagcgtat gctgccatac gttacctgcg gaatgtttaa
     4441 cccaccctac aggcgaaccg ctaactttac ttccattgga tgcatgattg ttgatctgga
     4501 tcatctatcg cagcatgggg ttaatgcgga ggagttaaaa gcaaggttaa aggttgaccc
     4561 ccgcgtactg ctgtgttttt gctcaccaag cgccgatgga ctcaagctat tattcaagct
     4621 cagccaaaag atttacgacg cagcaaggta cagcatggcc tataaaatgt ttattacaca
     4681 atttgcaaac cagcatggag ttgcgcattt ggttgatagg gttacctccg atgttacccg
     4741 tgccaccttc attagcattg atgccaatgc gtactttaat cctaacgccg aagcggtacc
     4801 cctgcataca cttataaact ttgatagccc cagctctatc aacaccgccc aaaagcaaat
     4861 acaagaattt gaatttagct tacctcctga aaatactgcc ccaaaagaaa aaaacatttt
     4921 accatccgat atcctccaaa aaataaagga aagcctgaac ccaaacataa aaacaaaggc
     4981 tgaaaaaatt atttacgtgc cacaggagct tgaacccgcc gtggaaaaag taaaaagccg
     5041 aatgcaggag ctgggcataa gcgttaaaca gattgagaat atccattacg ggaaaaaatt
     5101 tgtatttgag ctcgatggcc ggtttgcaca gcttaacctg ttttacggta agcaagggta
     5161 taaagtggtg gtaacaccag ttcgtaatgc cgatgccgaa cttgccgaag taagcagact
     5221 aatactttgc gaaattttta tgtaaccatg ccgcgcaaga aacgcatacc cctcacccta
     5281 accgaaaaac ttaaaaaaat taaaacagcc gggttaagtg ctaccctgcc acccaaccag
     5341 tacgacccat accaggagaa aattgacgac ctgaacaagc gcattgaacg agtgctagga
     5401 atctttaaca aaactgtaaa aacagcagag atggtttact ttataatgta cgacatagaa
     5461 aacgataagg ttcgaaacct aatagctaag taccttaaaa agaatggatg tgtaagggtt
     5521 caacgttcgg ttttcctaac atcatcggat agggaaaagt tttccgagat ccatcaaacg
     5581 ctgaaacagg tacaggagtt ttacgacaat accgatagta taataattgt tccggtaacg
     5641 cccgatagca taagaaccat gaaacttata ggacagaaca ttaacatcga ccttgcgctt
     5701 gggaacacca acaccctgtt tttttagcta ataacaaaaa ccaaaacaaa catggctaca
     5761 atttttgatt acgctcaaca gctccacaac ctgtgtgtag cagatcgata tagcgatgcg
     5821 cttaaactgt atgaaacaaa aatagtaaag caattcagac aaagggagat agatagaagt
     5881 ttcctggtta taaactgcgt taccaatgcc tactgcaaga caaaccaatg gaataaaggg
     5941 ataagctatg ggttaagtaa gctggattgt ttagcaagaa tgaggaccga tgttgattac
     6001 gccttaaaaa caattggttg gcaattgtta aaggcattga ttccaaatcc accaatggtt
     6061 aaacctgaaa ctttagattt tgaaaaggtt gagcaattgc tgaaaatgct taaggagagg
     6121 gccgcctgca gcgatatagc attaaagcta tacctggtat ggatcaggat gttaccagga
     6181 ttgcaggttg attatttaac aggacaacga ttttacaacc tgttccaacc ggaagaactg
     6241 attaactggc cagctgaagt gcccgaaaag caactcttta aacctattga ggtttggtac
     6301 ctgaaccact ccaagtacct ttaccagcac caacgatacc aggaatgctg caacctgtgc
     6361 gatatcgggt taaaccaagc aaatgctcta agcaaggatt gccaagcttg gctaacccga
     6421 aggtatgcat tagctttaaa gcaaatgggc gacatgaatc gcgctatcac cctaatggaa
     6481 gtgttagctc gaaagaaact ggactggttt atataccacg aactgggcga actatatttc
     6541 cagagtaacg atgcacaaaa tggcgaaaaa tgcctcgtcc tggcatacag gtttggtggg
     6601 cataacccga gcaaggttaa cctctacgag cgtttgggcg accattgcat gaatataagg
     6661 atgccaaata tagcgacaga attctatcaa cttgccctga aggtaagaac cgaagagggc
     6721 tggaaaatac ccgatagcct catgcaaaaa ctacccacca aaacaaccca tactgattcg
     6781 gcacagcttt tccgatcgct actaagggta ttccaaaaaa ccattgaggt gaacgacatg
     6841 ctttttggag ccggagaaat tacccgcata cttcatgagg gccccaatgg cgatggtttt
     6901 ataaccgata ccgagggtaa tacggtatac ttcaggtttt cccaagccaa ttttgactcc
     6961 gatgaggttg aaaatggata ccccgtaaaa tttaaagcaa gaagggttct acaccgaggc
     7021 gagaaacggt ggcaggctac caaggtgtac cgcgattaat tttaaaaaaa agttgttcaa
     7081 atgaaaatct tggaaaaaag tttttttaac tttgattgca agatgctgaa ataaaaatga
     7141 aaccaacttg taaaacaaaa ccaaaaagta tgtacctttg cacccccaaa aaaacaaccc
     7201 caaattcagc taaaaagttt aaagttttgt taaaaccgta ttgtaacata ctgttttaca
     7261 atagttttca aaaagtac
//
LOCUS       DAOSAL010000099         7137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_6732_length_7137_cov_46.550551, whole genome shotgun sequence.
ACCESSION   DAOSAL010000099 DAOSAL010000000
VERSION     DAOSAL010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 7137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7137
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6732_length_7137_cov_46.550551"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="PK049_10555"
     CDS             <1..611
                     /locus_tag="PK049_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK62041.1"
                     /translation="LKKSYYQIAREYNNKEAMQFFASNGIKTKIKPSITSVYFGIGTS
                     FSKHEFLLGYHLGVFEQITQTEFSLGYLYRPSATASLTSIGGQLYQYWEKRNILQLNI
                     RKYYNLFSYKGKNIISFAAIGAGPAFRNYRGTSSNPKTHFTTNFSGGLSIKGDAMEYS
                     IGVGYNFYKELGVSPYFYTISAKIHFNVLNPKVVYKNVKHVQ"
     gene            601..1023
                     /locus_tag="PK049_10560"
     CDS             601..1023
                     /locus_tag="PK049_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK62042.1"
                     /translation="MFSKIFKLLTILLIVLAPYSCEEMEFFIVNCDECYELKPDKIDV
                     RLKFTINNENQVVYFELYSGNPENGVLFFSGNTTISEKVFQFDANSYYSIKAYYTSKG
                     RQIIVIDATTVKLKYDKSSCSSPCYTISGDILDARLRY"
     gene            1161..2858
                     /locus_tag="PK049_10565"
     CDS             1161..2858
                     /locus_tag="PK049_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylase"
                     /protein_id="HOK62043.1"
                     /translation="MKRQLLIRDVTLRDGQQSLFATRMTQKQVERVLPYYREAGFYAM
                     EVWGGAVPDSVMRYLNEDPWYRLESIKAAIGDTSKLTALSRGRNLFGYNPYPEEVIEG
                     FNRNAVKSGISIMRIFDALNDTENIRSTVKYVKENGGMADCTVCFTVDPKFSRKDRIK
                     AMLSGKTLPRKIFTEKYYIAKAKELEAMGADMITLKDMAGLVTPEQSAAIISALKREI
                     KVPIDFHTHCTPGYGLASTLTAIVNGVDIVDTAIWNFAGGPAAPAFELIQIFCDKMGI
                     DTGVNLKAVASINAELKTIRTELKELDTYELPIDFDITSYNLPARIDNLFEQAILFAQ
                     SGKYDALLDACQAIERYFNFPEPDENVKNAEIPGGMYTNMLAQLKQLKLENLLPRVLE
                     IIPTVRLDAGCPPLVTPTSQIVGAQAVNCAIDESKGQPFYTNKSIQFVNLVKGIYGRT
                     PLPIDPQFRLKIAGVAEETPYDTSNYKKQPNPAFPEYGENVKLAMNEKEELLLELFPT
                     VAEKYLRDKVVNHYESIKRQKEEEVQRKIQEEKQKYYSMTEEQRWKRLLDGLQQYFC"
     gene            complement(2915..5416)
                     /locus_tag="PK049_10570"
     CDS             complement(2915..5416)
                     /locus_tag="PK049_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HOK62044.1"
                     /translation="MKKLFLLLLAVMANVLLVISQPVPVTKANYELPARFSPKKIDKM
                     VFSTSVDAHWLKKSTRFWYSYETPSGKSYYIVDPAKKSKSLLFDNVDMAAQLSRLTKE
                     PFDAQHLPIQKIKFIKNETVIQFEVKSTLVDEDKTDDEENDMKQKKDDKAKAKKDKKT
                     YYFEYDLNTRKLTLLDDYKKPKENPEWANISPNGEYVVYSKNHNLFWMDKENYEKAKK
                     NEKDSTIVEHQLTTDGEKYYSYGYNTKEEDNVEEVKNKDNRKPAYITWSHDSRYFAMV
                     RTDQRKVKDLWVIKTLSNPRPTLETYKYHMPGEKEAPQDEILVFDMNNRDKVIKPDIK
                     AFKDQDVSILNYRRLASERDDDYVPTRWLSKYNDRLYFQRTSRDLKRIDVCYVDIKTG
                     EVKTVIEERLNTYIDFNGIALINNEKEIIHWSERDGWGHFYLYDNQGNLIRQITSGPF
                     HCERLVGYNETTKTLFFTANGREPNEDPYYLHLYRIGIDGTGLRLLNKGNFDHSISMD
                     DNQQYIVNNYSRVNTIPESKLIDNQGNDLLFLEKADLTLLFEAGYKFPEPFTVKAADG
                     ITDLYGVMYKPFDFDSTKQYPIILYVYPGPQTEAVNKSFSARMDRTDRLAQFGFIVIT
                     VGNRGGHPARSKWYHNYGYGNLRNYGLADKKAATEQLAYRHKYIDINRVGIHGHSGGG
                     FMTAAAMLTFPDFFKVGVANAGNHENNIYNRWWSEKHHGVKEIADTAGNVTFEYNIDK
                     NSEIAKNLKGHLLITTGEIDNNVHPGNTIRLANALIKANKRFDFFMYPGQRHGYGDMT
                     EYHFWLTGDYFCKHLLGDYDTSIDITPMNNEIERK"
     gene            5516..7126
                     /locus_tag="PK049_10575"
     CDS             5516..7126
                     /locus_tag="PK049_10575"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HOK62045.1"
                     /translation="MQKLFILLLCIYPLLGNAQYFPVTVAEGSNFQSTSTYADVMNYI
                     KMLEKASKHIKTETIATSIEGRDIPLMVVANPMPKTPRDIGNRIVIYIQANIHAGEVE
                     GKEASLMFVRDLLKNPNNPLFKNIVLLVCPILNADGNESISPKNRPWQNGPVNGVGVR
                     HNGQMLDLNRDAMKLETPEMVGVVTNVLNRWDPSIVMDCHTTNGSYHQEPVTFTWMMN
                     PNGNRELINYMRDRMMPWVSTQLSVKYKTLNCFYGEFIDQKDYEKGWISYASEPRYFT
                     NYIGLRNRLSILNENYVYAPFDERVYGCYNLIASVCDYAMQNSKEIKDLLAKADREAI
                     QFPKTTPAPEFAITYEGRPTPQWVTILTYEAEAYTDEQGRERFRKTDRKRTVTVPYIA
                     DYYPTSTIPLPFAYIVRNDKVVTNLLKNHGIQFQTLTDTVTLNVEQFIIDSLKPAPRI
                     NQGHYNSNIFGKYVKANRLFTTNYVMVKTSQPLGQLAAYLLEPQSDDGLVYWNFWDRY
                     LVPQWGNNYNPVPVFRITDEKEFGRVKR"
BASE COUNT         2051 a         1611 c         1512 g         1963 t
ORIGIN      
        1 ctcttaaaaa gagttattac caaattgccc gcgaatacaa caataaggaa gccatgcagt
       61 tttttgccag taatggaatt aaaactaaaa tcaaaccatc aattacttca gtttactttg
      121 gcataggaac aagcttttca aaacatgagt ttctattagg ctatcatttg ggggtttttg
      181 aacaaataac ccaaacagag ttctcactcg gatacctcta ccggccatct gcaactgcct
      241 cgctaacttc catcggtggg cagctttacc agtattggga aaagagaaac atactacagc
      301 taaacatccg caaatactat aacctgttta gctacaaagg gaaaaacatt atttcgtttg
      361 cggcaattgg tgcaggacca gcgttccgga actacagggg aacatcctcg aacccgaaaa
      421 cacattttac aaccaacttt tctgggggtc tatcgataaa aggtgatgca atggagtatt
      481 cgataggtgt tgggtataat ttttataaag agcttggagt atcaccctat ttctacacca
      541 tatcagccaa aatccatttt aatgtgttaa atcctaaggt tgtatacaaa aatgtaaaac
      601 atgttcagta agatatttaa gttactaacc attttgctaa ttgttctggc gccctactcc
      661 tgtgaggaaa tggagttctt tatagttaac tgcgatgagt gttatgaatt gaaacctgac
      721 aaaattgatg ttcgcctaaa attcaccatt aacaatgaga accaagtagt ttactttgaa
      781 ctatacagtg gaaatcctga aaacggagtt ttgtttttta gtggaaatac taccataagc
      841 gaaaaagtat tccagttcga tgccaactcg tactactcaa taaaagcgta ttacacaagt
      901 aaaggaaggc aaataattgt aattgatgca actacagtta aactaaaata tgataagtcg
      961 tcgtgctcca gcccctgtta cacaatttca ggcgatatac ttgatgctcg cctcaggtat
     1021 taaaacatgt tttttagcaa tgttttaaaa ccatgttttc aaactactaa aaaccaaccg
     1081 ataattcaaa acaatattag ttacttcgcc gtaagtatta gtgtttagca agtatcagta
     1141 taaatataaa attgattacg gtgaagagac aactgcttat cagggatgta acccttagag
     1201 atggacagca atcgctgttt gccacccgaa tgactcagaa acaggttgaa agggtattac
     1261 cttactaccg tgaagccggg ttttacgcca tggaggtatg gggtggtgcc gttcccgact
     1321 ccgtaatgcg atatctgaac gaagatccat ggtataggct cgaaagcatt aaagctgcta
     1381 ttggcgatac ttcaaaactc acggcccttt ccaggggacg caatcttttt gggtacaacc
     1441 catatcccga agaggtgatt gaaggattta accgcaatgc ggttaaatcg gggatttcca
     1501 tcatgcgtat attcgatgcc cttaacgata cagagaatat tcgcagcacc gtaaagtatg
     1561 taaaggaaaa tggaggcatg gccgactgta ccgtttgctt taccgttgac cctaaatttt
     1621 cccgtaagga taggattaaa gccatgctaa gcggtaaaac cttaccccgt aaaatcttta
     1681 ctgaaaagta ttatatagct aaggccaagg agctcgaggc catgggtgcc gatatgatta
     1741 ccctaaaaga tatggccgga ttggttaccc ctgagcagtc ggctgcaatt atttcggcgc
     1801 taaaacgcga gattaaagtg cctatcgatt tccatacgca ctgtacaccg ggttatggtt
     1861 tggcatcaac ccttacagcc attgtaaatg gggttgatat tgttgatact gccatatgga
     1921 attttgctgg tggtccagca gcaccagcat ttgaacttat tcagattttc tgcgataaaa
     1981 tggggataga tactggtgtt aacctgaagg ctgtagccag tattaacgca gaacttaaaa
     2041 ccattcgcac agagctaaag gaacttgaca cctacgagtt acccattgac tttgatataa
     2101 ccagctataa tctccctgca cgtatcgata acctgtttga gcaagccatt ctgtttgccc
     2161 agtcgggcaa gtacgatgcc ctacttgatg cttgccaggc catagagcgt tactttaatt
     2221 tccccgaacc cgatgagaac gttaaaaacg ctgagattcc ggggggaatg tacaccaata
     2281 tgctggcaca gctcaagcag ctaaagcttg aaaacctact tccaagggtg ctagaaatca
     2341 tcccaaccgt aaggctcgac gccggttgcc ctccgctggt tacacccacc agccagattg
     2401 ttggtgcgca agccgtaaac tgtgctattg acgaaagtaa aggacagccc ttctacacca
     2461 ataaatccat acagttcgta aaccttgtta aagggatata cgggagaact cccttaccaa
     2521 ttgacccgca attccgcttg aagattgccg gcgtagccga ggaaacccct tacgacacat
     2581 ctaactacaa aaaacagcca aacccagcgt tccctgaata tggtgagaat gtgaaattgg
     2641 ccatgaacga gaaggaagaa ttgctgcttg agctattccc taccgtagcg gaaaaatatc
     2701 ttcgcgataa agtggtaaac cattatgagt caattaagcg ccagaaggag gaagaggttc
     2761 agcgtaaaat tcaggaggaa aaacaaaagt actactcaat gaccgaggaa caacgctgga
     2821 aaaggcttct ggatggatta cagcaatatt tctgttaact tcaataggtt taggttaaaa
     2881 gttaggttga aaaaaagggg ctcttgcccc ttttttattt tcgttctatt tcgttgttca
     2941 tgggtgttat atcaatactc gtgtcgtaat cgcccagcaa atgcttgcag aagtaatcgc
     3001 cggtaagcca gaaatggtac tcggtcatgt cgccatagcc atgtcgctgg ccagggtaca
     3061 tgaagaaatc gaaacgttta ttggccttaa tcagagcatt ggcaagccta atggtgtttc
     3121 ccggatgaac gttattatct atttcgccag tagtaataag tagatgccct ttaaggttct
     3181 tagcaatttc agagttctta tcgatgttgt actcaaacgt tacattaccg gccgtatcgg
     3241 caatttcctt aacgccatgg tgtttttcac tccaccagcg gttgtagatg ttattctcgt
     3301 ggttgccggc attagccact ccaaccttaa agaaatcggg gaaggttagc atggctgctg
     3361 cagtcataaa accgccacct gaatggccat ggatacccac acggttaata tcaatatatt
     3421 tatgtcgata tgccaactgc tcagtagcag cctttttatc cgctaagcca tagttgcgca
     3481 ggttgccata gccatagtta tgataccact ttgaacgagc agggtgtcca ccccgattac
     3541 caacggtaat aactataaat ccgaactggg ccagcctatc ggttctatcc attcgtgcgg
     3601 agaacgactt attcaccgcc tcggtctgtg ggccagggta cacgtaaagg ataatggggt
     3661 attgtttagt agaatcgaaa tcgaaaggtt tgtacattac gccatacaaa tcggtaatgc
     3721 catcggcagc tttaacggta aatggctcgg ggaatttgta gccagcctcg aatagcagtg
     3781 ttaaatcagc cttttcaagg aaaagtaaat cgttgccctg attatcaatc aatttggact
     3841 ccggaatagt attaacccgc gaatagttgt tcactatata ctgctgatta tcgtccatgc
     3901 tgatggaatg gtcaaaatta cccttattca gcagccttag gccagtacca tcaataccga
     3961 tccgatacag atgaaggtag tatggatcct cattgggttc acgtccattt gcggtgaaga
     4021 acagcgtttt ggtggtttcg ttgtagccaa caaggcgctc gcaatggaat ggtccactgg
     4081 ttatctgcct tatgagattt ccctggttat cgtacaggta aaaatgcccc caaccatcgc
     4141 gctccgacca gtgaataatc tctttttcat tattaatcaa cgcaatgcca ttgaaatcga
     4201 tataagtgtt taagcgctcc tctataaccg ttttaacctc acctgtttta atatcaacat
     4261 agcaaacatc aatccgcttg aggtcgcgcg atgtacgctg gaagtagagc ctatcgttgt
     4321 acttggagag ccatcgggtt gggacatagt catcgtcgcg ttcactggcc aatcgacggt
     4381 aattcaggat tgaaacatcc tgatccttaa atgctttgat atcgggtttt atcaccttat
     4441 ccctattatt catatcaaaa acgagtatct cgtcctgtgg ggcttccttt tcacctggca
     4501 tgtggtactt ataggtttcc agggttggcc tgggattgct aagagtcttg atcacccata
     4561 aatccttaac cttacgctgg tcggtacgaa ccatggcgaa gtaacgcgag tcgtgcgacc
     4621 atgtaatgta agctggctta cggttgtcct tattctttac ctcctctaca ttatcctcct
     4681 ccttggtatt atacccgtag ctatagtact tctcaccatc agtggttagc tgatgctcaa
     4741 caatggtgga atccttctcg tttttcttag ctttttcgta gttctccttg tccatccaga
     4801 atagattgtg gttcttgctg taaaccacat actcgccatt aggcgatata tttgcccact
     4861 caggattttc ctttggtttc ttataatcgt cgagcagggt tagcttgcgg gtgttcaaat
     4921 cgtactcaaa gtagtacgtt ttcttgtcct ttttggcctt ggctttatcg tcctttttct
     4981 gcttcatgtc attctcctca tcgtcggttt tatcttcatc gaccaacgtg cttttcacct
     5041 caaactgaat gacagtctcg tttttaatga acttgatttt ttgtataggt agatgctgcg
     5101 catcgaacgg ctccttggtg agccttgaga gctgagcagc catatcaaca ttatcgaaca
     5161 gcaacgactt gctctttttt gccggatcaa ctatgtaata gcttttgccc gaaggggttt
     5221 cgtaactata ccagaaacgc gtactcttct taagccagtg ggcatcaacg ctggtgctaa
     5281 aaaccatttt atcaattttt ttgggcgaaa atctggcggg tagctcgtaa ttagctttgg
     5341 taacaggtac aggttggcta atcacaagca atacgtttgc cattactgcc aatagtaaca
     5401 gaaaaagctt tttcatgatt ttagagtttt gggttgtttt actaactttg attacaacag
     5461 ctaagctgag tttaattaaa ctttgaacaa attgaaatat caaacaaata aaaaaatgca
     5521 aaaactattt attcttctgc tttgtatcta tccactgctc ggaaacgccc aatactttcc
     5581 ggttacagta gctgagggta gcaactttca gtcaacatca acctatgccg atgttatgaa
     5641 ctacatcaag atgcttgaga aagcatcgaa gcacataaaa accgaaacca ttgccaccag
     5701 cattgaggga cgcgatatcc cgctaatggt tgtggcaaac ccaatgccca aaacaccccg
     5761 cgatattggt aaccggattg tgatatatat acaggctaac atacatgcgg gggaggtgga
     5821 aggcaaggaa gcatcgctga tgtttgttcg cgacttgctt aaaaacccaa ataatccact
     5881 attcaaaaac attgttctgc tggtttgccc tattctcaat gccgatggta acgaatccat
     5941 cagtccgaaa aaccgccctt ggcagaacgg gcctgttaac ggtgttggcg taagacataa
     6001 cgggcaaatg ctcgacctga acagggatgc catgaaactg gaaactcccg aaatggttgg
     6061 cgtagtaacc aacgtattaa accgctggga tccttccatt gttatggatt gccacaccac
     6121 caacggatcc taccatcagg agccggttac ctttacctgg atgatgaacc ccaacggtaa
     6181 tcgcgaactc ataaactaca tgcgcgatag gatgatgccc tgggtatcga cccagctatc
     6241 ggtcaagtat aaaacgctaa actgcttcta cggtgagttt atcgaccaaa aggattacga
     6301 aaagggttgg atatcgtacg cttctgaacc ccgctacttc accaactaca ttgggttaag
     6361 gaataggctt agcattttga acgagaacta cgtttacgcc ccattcgacg aacgggttta
     6421 cgggtgctat aacctgattg cctcagtttg cgattacgcc atgcaaaaca gcaaggaaat
     6481 aaaggatttg cttgccaaag ccgacagaga ggctattcag tttcccaaaa ccacacccgc
     6541 tcctgagttt gccataacct acgagggtag gcctacgccc cagtgggtaa ccatcctcac
     6601 ctacgaggcc gaagcctata ccgacgagca gggacgggaa cgcttccgca aaaccgaccg
     6661 taagcgcacg gttactgttc cctacattgc cgactactac cccacctcaa ccattccgct
     6721 cccctttgcc tacattgtta gaaacgacaa agtggttacc aatctcctta aaaatcacgg
     6781 aatacaattc caaaccctaa ccgatacggt aaccctaaat gtagagcagt ttatcatcga
     6841 ctcgctaaag ccggcccctc gaattaacca ggggcactat aacagcaata tctttggcaa
     6901 atacgttaag gccaataggc tattcacaac caactacgtg atggttaaaa cctcgcaacc
     6961 ccttgggcaa ctggctgctt acctgcttga gccacagtcg gacgatgggt tggtttattg
     7021 gaatttctgg gaccgttacc ttgtgccaca atggggcaac aactacaacc cggtaccggt
     7081 tttcaggata accgatgaga aagagtttgg cagggtaaag cggtagcttt tagcgca
//
LOCUS       DAOSAL010000100         7100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_6779_length_7100_cov_49.159972, whole genome shotgun sequence.
ACCESSION   DAOSAL010000100 DAOSAL010000000
VERSION     DAOSAL010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 7100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7100
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6779_length_7100_cov_49.159972"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PK049_10580"
     CDS             complement(<1..233)
                     /locus_tag="PK049_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF21 domain-containing protein"
                     /protein_id="HOK62046.1"
                     /translation="MGTQLLVIAVAILFSAFFSGVEIAFLSSNKLRFELERKHGRLTS
                     RILDIFYRNKEQFIATMLVGNNITLVIYGIVTA"
     gene            complement(240..425)
                     /locus_tag="PK049_10585"
     CDS             complement(240..425)
                     /locus_tag="PK049_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK62047.1"
                     /translation="MNKLAFILELIWLALALFCLGMGIWATYKGLSNSYMFFVMAVVA
                     LLMYFLRRSRRKKMENQ"
     gene            complement(518..1090)
                     /gene="lptC"
                     /locus_tag="PK049_10590"
     CDS             complement(518..1090)
                     /gene="lptC"
                     /locus_tag="PK049_10590"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HOK62048.1"
                     /translation="MIRLNIKVFKNMVVLTLVGATSLFFVSCKGDSEAIVYLSDIKTT
                     PGVTAYNSEVVYTEGGKVVLKVIAPETVYYQFADEPYTEFPKGITVYTYDNELKQESS
                     LTANYAIYYEKKLLWQAKNNVVAKNRKGEQLYTEELYWDQNKRIIYTNVNVKINSVSG
                     IIYGKGMIADENFDNWEVKEPYDGEFEVDQ"
     gene            complement(1093..>2337)
                     /locus_tag="PK049_10595"
     CDS             complement(1093..>2337)
                     /locus_tag="PK049_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK62049.1"
                     /translation="KYGPDEESRKECAKNLSLYGTFYQQSNYKDAYKPWQNVIRICPA
                     ASINTYIRGVKLLKARYEEVADPAQKKVLVDSLMMIYDLRITHFDKKGMVLGQKAEDL
                     FTLAPDRYEEAFNIAKEAISIEKANSNPSTLFAYVSIAKAMYDNKKLTADQVIELYSQ
                     VSDYIDAQVTANPSDDKAKQAKDGIDAIFAGMGVANCDNVIALFEPRFKATPEDVNLC
                     KKIRSLMSANRCTSHPLYLNASIQIFKGEPNADLALDIARLQYEAKNAREAEKYYNEA
                     INLESAVEKRANIYYELALLTFTELKNLPKAREIALKAIEENPNMGKAYKLIGDIYAS
                     ERNCGSNDFEKKTVYWVAVDKYIKAKQVDPELTDAMDNLISTYSQYYPSKEDIFFYDL
                     KVGDSYTVGCWINERTTVRDRK"
     assembly_gap    2338..2437
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2438..3255)
                     /locus_tag="PK049_10600"
     CDS             complement(<2438..3255)
                     /locus_tag="PK049_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK62050.1"
                     /translation="MGGVSQPYRSETIINFNNPASYAQRDSMSFLFDFGLETNTSGFT
                     GYDQYLNPQTSRNSAGGIHHIAISFPIAKRIGLAAGITPYSSVGYRLLRFETDPYILS
                     TIGRVKYYHTGRGGINQAFVGMGVSPLKNLSVGFNMLYYFGSLDYNNDIEFPLNYNPY
                     TNGYIRSSFQVSDINFNFGAQYSLVLDKENNTNITLGGTYQLGKNINMTQKWFATLES
                     NLLDTLYPHPDRQSKLELPWSVNAGLAFTYKDKFTIMGEYFTQDWSKTTPFNVNT"
     gene            complement(3401..4120)
                     /locus_tag="PK049_10605"
     CDS             complement(3401..4120)
                     /locus_tag="PK049_10605"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOK62051.1"
                     /translation="MNLIIDIGNTLSKVAIADGKEVRFFQSFHELDIDDLMPIINDFK
                     PERCIVSAVGKIPVNTLVWLRQNLPVHIAGANTNIPLKNDYSTPETLGFDRLALAVGA
                     NYLFPNQNVLVIDAGTAITYDLVTSEGVYKGGAISPGLRLRFKALNEYTAKLPLIEHF
                     KTVDIVGTSTIECISSGVVNGTVLEIEGYVGKINSIYEGCAVVLTGGDANFLANMLKN
                     SIFVNPFLMVIGLNRILEFNV"
     gene            complement(4137..>7100)
                     /gene="mfd"
                     /locus_tag="PK049_10610"
     CDS             complement(4137..>7100)
                     /gene="mfd"
                     /locus_tag="PK049_10610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOK62052.1"
                     /translation="IVTYSDALFEKIPLRNILKENILTISKGERISTDFITEVLFEYG
                     FERTDFVYEPGQFAIRGSIVDVFSYSSAMPYRIDFFGDEVESIRTFDVEDQLSRDLHQ
                     TISIIPNLQEKEQAEGAILTDELPENSTVWVKNPELVLERINQLNEIALQIDFKAQAG
                     YASGKEFFTSLQNHRIVYFGLRPPSTVHQNIEFSTSPQPAFSKNFELLADDIEQKNLN
                     GYRVYLLSENQAQLDRLNNILHSIKPDLHFELINESIAEGFVDHSLRICLYTDHQIFD
                     RYHKFRLKHEFTRRDALTIQELVNLQPGDYVVHIDHGIGVFGGLVKTEVNGRLQEAIR
                     LIYQDNDTLLVSIHNLHKISKYRGKDAEPPKVYKLGSGAWQKLKQRTKSKIKDIAKDL
                     IALYAKRKAQKGFAFSPDTYMQQELEASFIYEDTPDQQKATQAVKLDMEDERPMDRLV
                     CGDVGFGKTEVAIRAAFKAVADSKQVAVLVPTTILALQHYRTFTSRLNGFPCNVEFIS
                     RMKSAAQQREIRQRLKDGKIDILIGTHSILKEASNFKDLGLLIVDEEQKFGVAAKEKI
                     RQFKLNVDTLTLTATPIPRTLQFSLLGARDLSIINTPPPNRYPIQTELHVFNTAIIKE
                     AIEYEVSRGGQVFFVHNRVQNIGEVQIMLHKIVPKIKTAVAHGQLEPSNLESIMLDFI
                     NGDYDVLISTAIIEAGLDIPNANTIIINNAHMFGLSDLHQLRGRVGRSNKKAFCYLLA
                     PPLESLTPEARRRLKAIEEFSELGSGFSIAMQDLDIRGAGNLLGAEQSGFISEIGYET
                     YQRILDEAIQELKETNFRELFSPQQAETRTDKWVGSNTDCQVETDLEVLIPDYYVSNT
                     AERIKLYRELDSIKNSQELEVFASELRDRFGPIPDPVIELMSIVRLRWIAVELGIEKI
                     ILKNGKAHAYFITNQLSPFYRTSTFERIINYILRNPGIFSMKEGNEKLILTIKDVKGV
                     GKAIDILQQMLDNVR"
BASE COUNT         1948 a         1539 c         1376 g         2137 t
ORIGIN      
        1 tttgctgtta caataccata aattaccagg gtgatgttgt tgcctaccag catggtggca
       61 ataaactgct ccttgttccg ataaaagata tcgagaattc gagaggttaa acggccatgc
      121 ttccgttcca actcaaagcg taacttattt gatgatagaa aagctatctc aacacccgaa
      181 aagaaagccg aaaacaaaat agcaaccgct attaccagta gctgtgttcc catcttttac
      241 tattggttct ccattttctt tcgcctgctt cgtcgcaaaa aatacatgag taaagcaact
      301 acagccatta caaaaaacat gtagctgttg cttaatcctt tgtaggtagc ccaaattccc
      361 atacccaaac aaaatagggc aagggcaagc cagattaact ctaggataaa tgccaactta
      421 ttcatgtaaa aaagtatgta tggtttacaa taggtaaagg tacaaaaaaa ggattgtatg
      481 tcaatacaac ccttaaatgc tttaaacgat ttaattatta ctggtcaacc tcaaactcgc
      541 catcataggg ctctttaact tcccagttat caaaattttc atcggcaatc attccctttc
      601 catatataat gccgctaacg ctattaattt taacattaac gttagtataa atgatgcgtt
      661 tattctgatc ccagtaaagt tcttccgtgt aaagctgttc gcccttcctg tttttagcca
      721 ctacgttgtt ctttgcctgc caaagcaatt tcttttcgta gtaaatggca tagttagcgg
      781 taaggctcga ctcctgcttt aactcattat cgtaagtgta aactgttatc cctttaggaa
      841 actcggtgta gggctcatcg gcaaactggt agtaaaccgt ttcaggggct attactttta
      901 aaaccacttt acccccctca gtgtaaacta cctctgaatt ataggctgta acaccgggtg
      961 tagtttttat atcggaaagg taaactatag cctcagaatc gcccttacaa gaaacgaaaa
     1021 ataaagaagt agcccccacc agggtgagga ctaccatatt cttaaatact tttatgttta
     1081 gcctaatcat ttttactttc tatcccttac agttgtacgc tcattaatcc agcatcctac
     1141 tgtgtatgaa tcgccaacct ttaaatcgta gaagaagatg tcctcttttg aggggtagta
     1201 ttggctgtaa gtgcttataa ggttatccat cgcatcggta agttcaggat caacctgctt
     1261 tgccttaatg tatttatcca ctgcaaccca gtaaacagtc ttcttttcaa aatcgttgct
     1321 gccacagttg cgctcgctgg catatatatc gccaatcagt ttataggctt tacccatgtt
     1381 gggattttcc tcaatggcct ttaatgcaat ttctctggct ttgggtaagt tcttaagttc
     1441 agtgaaagtg agcagtgcaa gttcatagta aatgttggct ctcttttcta cagccgattc
     1501 aaggttaatt gcctcattgt agtacttttc tgcctcccgg gcattctttg cttcgtactg
     1561 cagcctggca atatcaaggg ctaaatcagc attaggttca cccttaaaaa tttgtatgct
     1621 ggcatttaag tatagtgggt ggctagtgca cctatttgcc gacatcagcg agcgaatttt
     1681 tttgcaaagg tttacatcct ctggagtagc cttaaaacgg ggctcaaaaa gagcaataac
     1741 attatcgcag ttagctacac ccatgcctgc aaaaatagca tcaattccat ccttagcctg
     1801 ttttgcttta tcatcgctgg ggttcgctgt cacctgagca tcaatatagt cgctcacctg
     1861 tgagtaaagt tcaattacct gatcggcagt caactttttg ttgtcgtaca tagcctttgc
     1921 aattgaaaca taggcaaaga gtgttgatgg gtttgaatta gccttttcaa tgctaatagc
     1981 ctccttggca atgttaaaag cctcctcgta acggtccggt gcaagggtaa ataaatcttc
     2041 agccttctgg ccaagaacca tacccttttt atcaaaatgg gtaatacgga ggtcgtaaat
     2101 catcatcagt gagtcaacta agactttctt ctgggcagga tcagcaacct cctcgtaacg
     2161 agcctttaaa agctttacgc ctctaatgta tgtgttaata ctagctgcgg gacaaatcct
     2221 aataacattc tgccaaggtt tataggcatc cttatagttg ctttgctggt agaatgtccc
     2281 gtaaagcgat aggtttttag cacattcctt ccgggattcc tcatcgggcc catacttnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngtg tttacgttga aaggggtggt
     2461 ttttgaccag tcctgagtaa agtattcacc cataatagta aacttatcct tataagtaaa
     2521 tgctaaacca gcattcacgc tccagggtaa ttcaagtttc gattgcctat cgggatgagg
     2581 gtaaagtgtg tcgagtaggt tactttcaag ggttgcaaac catttctggg tcatatttat
     2641 gtttttccca agctggtagg ttcccccaag tgtaatattt gtattgtttt ccttatcgag
     2701 aacaagcgaa tactgtgctc caaagttaaa gttgatatcg cttacctgaa agctggatcg
     2761 aatatacccg ttggtgtaag gattatagtt aagaggaaat tctatatcgt tgttgtaatc
     2821 aaggcttcca aagtagtata gcatattaaa cccaaccgat aagtttttta agggacttac
     2881 ccccattcca acaaatgcct ggtttatacc tcctctgcct gtatggtagt actttaccct
     2941 tccaatggta cttaggatgt aaggatcggt ctcgaaacga agtaacctat accctacaga
     3001 gctgtatggt gttatacccg cagctaatcc aattcgctta gctataggga aagagattgc
     3061 aatatggtga atgccacctg cactattccg cgatgtttgt ggattaagat actggtcgta
     3121 acctgtaaaa ccgctagtgt tggtctccaa gccaaaatcg aacaggaacg acatactatc
     3181 cctttgggcg tatgaagcag ggttgttgaa gttaataatg gtttcgctcc tatagggttg
     3241 cgatacgcct cccattgaac ggttttgagt aaacccaggt tgactcagca ctccaatgcc
     3301 ataccgagag tatggtgaat aggtgttgag gctctgtgct cctaaaaatt cagataggca
     3361 taatagccca gccaaaatca aaaaacgcgg aaatgctaat ctatacattg aattcaagta
     3421 ttctgtttaa accaattacc attaaaaaag ggttcacaaa tatgctattt tttaacatat
     3481 tagccaagaa atttgcatcg ccacctgtta aaaccacagc acaaccctca taaattgagt
     3541 taatttttcc gacataaccc tcaatttcca gaactgttcc attaaccact ccggaactta
     3601 tacattcaat cgtggatgtt ccaacaatat caacagtttt aaagtgttca atcaacggta
     3661 acttagcagt atattcgttc agcgccttaa agcgtaatcg taacccaggc gatatggcac
     3721 ctcccttgta taccccttca ctagttacta aatcgtaggt tatggctgtg ccggcatcaa
     3781 ttactaaaac gttctggttc gggaaaaggt agtttgctcc aaccgcaagc gcaagcctat
     3841 cgaatcctaa ggtttcaggg gtagagtaat cgttttttag ggggatgtta gtatttgcac
     3901 cggcaatatg cacaggcaag ttttgcctta accataccaa ggtgtttacg ggtattttac
     3961 caacagccga aacaatgcat ctctcgggct taaaatcatt gataattggc attaaatcgt
     4021 caatatcaag ctcatgaaag ctctggaaga atcttacttc ctttccatcg gctatggcaa
     4081 ccttgcttaa agtatttcct atatcaataa ttaggttcat aatttgcttt aaattactaa
     4141 cggacattat cgagcatttg ttgtagaata tcaatagcct tacctactcc ttttacgtcc
     4201 ttaatggtta aaatgagctt ttcgttaccc tctttcatgg aaaaaattcc tgggttacgc
     4261 aaaatatagt taatgattcg ttcaaaagta gatgttctgt aaaacggtga tagctgattg
     4321 gtaataaagt aggcgtgagc tttaccgttt ttgagaataa tcttttctat tcccaattct
     4381 acggctatcc atcgtaacct cacaatgctc atcaattcaa taacaggatc gggaattggg
     4441 ccaaacctat cgcgaagctc gctagcaaat acttcaagtt cctgagagtt tttaatggaa
     4501 tcaagctctc tgtaaagttt aatacgctca gcagtattcg aaacgtagta gtcggggatt
     4561 aatacctcca ggtccgtttc aacctggcaa tcggtattgc tacctaccca tttatcggtt
     4621 ctagtttcag cctgttgggg tgaaaatagt tcacggaaat tggtttcctt tagctcctga
     4681 attgcctcat cgagtattcg ctggtaggtc tcataaccta tttccgaaat aaaaccgctc
     4741 tgttcagcac cgagtaggtt tccggcacct ctaatatcca aatcctgcat ggcaatacta
     4801 aatccgctcc ccagctctga gaactcctca atggctttta acctccgcct tgcctcaggt
     4861 gttagcgact cgagcggcgg agccagcaag tagcagaagg cctttttatt agaacgtcct
     4921 accctgccgc gaagctgatg caaatcgctt agcccaaaca tgtgggcatt gttaattatt
     4981 atggtgttag cattaggtat atcgagccca gcctcaatga tagctgttga gatgagcaca
     5041 tcgtaatcgc catttatgaa atcgagcata atactttcaa gattcgatgg ctccaattgc
     5101 ccgtgagcaa cagccgtttt aattttgggg actatcttgt gcagcattat ctgcacttcg
     5161 ccaatgtttt gaacccggtt atgcacaaaa aaaacctgcc ctcccctgga aacctcatat
     5221 tcaattgctt cctttattat tgctgtgtta aaaacatgca actcggtttg aatgggatat
     5281 ctattaggtg gcggggtgtt gattatcgat aggtcccggg ctcccaacag tgagaattgc
     5341 aaagttcgcg gaataggtgt tgccgttaag gttaaagtat cgacattcag cttgaactgc
     5401 cttatttttt ccttagccgc tacgccaaac ttttgttctt catcaacaat aagcagccct
     5461 aaatctttaa agtttgatgc ttctttaagt atggagtgtg tgccaataag tatatctatt
     5521 ttaccgtctt tcaagcgttg cctaatctct cgctgttgtg cggctgattt catcctgctt
     5581 ataaactcaa cattgcaggg aaaaccattg agcctcgatg taaaggttct gtaatgctgt
     5641 aatgctagta tggtagttgg caccagtacg gccacctgct tgctatcggc aactgctttg
     5701 aaagcggctc taatagccac ctcggtttta ccaaaaccaa catcgccaca cacaagccta
     5761 tccattgggc gttcgtcctc catgtcaagt ttaacggcct gggttgcttt ctgctggtca
     5821 ggggtatcct cgtatataaa tgaagcctca agctcctgct gcatgtaggt atcgggcgag
     5881 aaagcaaaac ccttctgagc ctttcgcttt gcataaagcg caataaggtc ctttgcaata
     5941 tcctttattt tgctcttggt gcgctgtttc aacttttgcc aagcccccga ccctagcttg
     6001 taaacctttg gtggctcagc atccttaccg cgatacttgg atattttatg aagattatga
     6061 atgcttacaa gcaatgtgtc gttatcctga tagataagcc gaattgcctc ctgtaatctt
     6121 ccatttacct cggtttttac caaacctcca aaaactccaa ttccatgatc gatgtgtaca
     6181 acataatcgc ctggttgcag gtttaccagc tcctgtatgg tgagtgcatc gcgacgggta
     6241 aattcgtgtt ttaaacggaa cttatggtaa cggtcaaaga tttggtgatc ggtgtagagg
     6301 caaatcctca agctgtggtc aacaaaaccc tccgcaatgc tctcgttaat tagctcaaag
     6361 tgcaagtcgg gctttatgga atgtaaaatg ttgttgagcc tatcgagctg agcctgattc
     6421 tcggacagca gataaaccct gtaaccattt aaattttttt gctcaatatc atcggccagc
     6481 agctcaaagt tttttgagaa tgcaggctga ggtgaggttg aaaattcaat gttttggtga
     6541 accgttgagg gtggccttaa accaaagtaa actatccgat ggttctgaag tgaggtaaag
     6601 aactccttgc cagaagcata tcccgcttga gccttaaaat caatctgtaa tgctatctcg
     6661 tttagctggt ttatcctttc aagcaccaac tcagggttct ttacccacac cgttgagttc
     6721 tcgggtaact catcggttaa gatagcccct tcagcttgct ccttttcctg taggttagga
     6781 attattgaaa tggtttgatg taagtcgcga gagagctggt cctccacatc aaaggttcgt
     6841 atggactcaa cctcatcacc aaaaaaatcg atacggtaag gcattgccga cgagtacgag
     6901 aaaacatcaa caatgcttcc tcttatggca aactgacccg gttcatatac aaaatcggtt
     6961 cgttcaaaac catactcaaa aagaacctca gtaatgaaat cggttgatat cctttctcct
     7021 ttcgatattg ttaaaatatt ctccttcaaa atattcctaa ggggaatttt ttcaaaaagc
     7081 gcatcgctat aggttactat
//
LOCUS       DAOSAL010000101         6827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_7152_length_6827_cov_47.002363, whole genome shotgun sequence.
ACCESSION   DAOSAL010000101 DAOSAL010000000
VERSION     DAOSAL010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 6827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6827
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7152_length_6827_cov_47.002363"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="PK049_10615"
     CDS             complement(<1..520)
                     /locus_tag="PK049_10615"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HOK62053.1"
                     /translation="MAENVFGKPEKLSNQWKTNIKSVKAYKTGFELTQPIIELNTDET
                     ITLEFDDLSDETEQYEYTVIHCDYSWNPTNMVFMEYADGFDFNPIHDYRYSSGTLVQY
                     RHYTLTIPNSNLKLKLSGNYIVQVVRQSNRSEIVLQQQFRVYEPLVKVDALIRQPIEP
                     SIQSTHQQMELTV"
     gene            726..2084
                     /locus_tag="PK049_10620"
     CDS             726..2084
                     /locus_tag="PK049_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HOK62054.1"
                     /translation="MSKPIKIKRGLNIPLKGSAEKVLVRLDKAETYGVKPTDFPGLVP
                     KLNVAVGDKVRAGSPLFHDKYRPNIVFSSPVSGEVVDVRRGERRVILEVVVKADSGEV
                     AYEDFGPASPETLTRDEVIAKLLKAGAWPYIRQRPFGIIANPADSPKSIFISGFDTSP
                     LAPDLDFVVQGEEESFQAGVDAIRKLTTGKVHLGLSSDFPPAKAFANAKGVEFHYFTG
                     PHPTGNVGVQIHHIDPINKGEVVWVVNPLDVIIIGRLFLKGIYDATKVIALAGSEVVK
                     PRYYKLISGASIESIIKDNINTQGQVRIISGNVLTGTRVDKLGYLGFYDNMVTVIPEG
                     NKYEFFGWIAPGVNKHSASSTFLSKLIPGKRFTLDTNMHGGHRAMVVTGQYEKVMPMD
                     IYPMHLVKAILANDIDRMEQLGIYEVVEEDMALCEYVCPSKTEVQAILRKGIETMIKE
                     LS"
     gene            2101..3267
                     /locus_tag="PK049_10625"
     CDS             2101..3267
                     /locus_tag="PK049_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HOK62055.1"
                     /translation="MKALRNYLDKIKPHFQKGGRFEMFHSTFDAFETFLFVPNTVTTK
                     GSHIRDSLDLKRTMSIVIIALMPAMLFGMWNVGYQHALSIGASWGFLETFWYGFLKVL
                     PLIVVSYGVGLSIEFAFAQSRGHEVNEGFLVSGLLIPLIMPVDVPLWMLAIAVAFAVI
                     IGKEVFGGTGYNVFNPALLARAFLFFAYPSHMSGDKVWIAGLTQGKGVVDGFSGATPL
                     AKAAAYQIDQLPSVYDMFMGFIPGSVGETSTLAILIGAIILIVTGIGSWKIMVSVFGG
                     GLAAGLLFNAFAVNPYMEIPAWQHLIMGGFAFGAVFMATDPVSASQTEKGKWIYGFLV
                     GFLALLVRVLNPAYPEGMMLAILLMNAFAPLIDYYVVEANIRRRMKRAKKVANV"
     gene            3280..4053
                     /gene="nqrC"
                     /locus_tag="PK049_10630"
     CDS             3280..4053
                     /gene="nqrC"
                     /locus_tag="PK049_10630"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit C"
                     /protein_id="HOK62056.1"
                     /translation="MSLLDKIKSMDRNSNAYTIMYSSVMVIVVAMLLAIASTALKPAQ
                     TKNIETEKKIDILRSIGKADGVEQAANKHAFVSENFNKYIPEQVVVNSKGDTITGVVA
                     FNVDLKVEMSKPIEQRNLPVYKAKLDDGSIKYIIPLRGRGLWGPIWGYIALNDDLNTI
                     YGATFAHQGETPGLGAEISTHEFQEQFKGKSIFQGDSILTFTSVKVVKGGAAPNDPHG
                     VDAISGGTITSKGLESMLFDCLAAYQSYFQKVKNSISHE"
     gene            4046..4705
                     /locus_tag="PK049_10635"
     CDS             4046..4705
                     /locus_tag="PK049_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HOK62057.1"
                     /translation="MSEVKEREPWFSPKNIKLLTEPIDSKNPVTVLVLGICSALAVTV
                     KLKPAIVMAISVTVVTAFSNLIISLIRNSIPNRIRIIVQLVVIAALVILVDQILKAYV
                     YDVSKQLSVFVGLIITNCIIMGRLEAFALGNKPWPSFLDGIGNGLGYGLILVIVAFFR
                     ELLGSGTIFNYRVIPIDWYIKNGGFYENNGMMILPPMALITVGIIIWIQRSRNRKLIE
                     D"
     gene            4728..5345
                     /gene="nqrE"
                     /locus_tag="PK049_10640"
     CDS             4728..5345
                     /gene="nqrE"
                     /locus_tag="PK049_10640"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HOK62058.1"
                     /translation="MEHLINIFVKSIFVDNMVFAYFLGMCSYLAVSKTVKTSMGLGIA
                     VIFVMAITVPVNYLMNKYLLLPGALSWLDPGFANLDLSFLSFIMFIAVIASMVQLVEM
                     VVEKFSPSLYNSLGIFLPLIAVNCAILGGSLFMQEREYETLAEATVFGVGSGIGWALA
                     IVSIAAIREKLKYSDIPKPLRGLGIAFILTGLMGIAFMTFMGIKL"
     gene            5367..6626
                     /gene="nqrF"
                     /locus_tag="PK049_10645"
     CDS             5367..6626
                     /gene="nqrF"
                     /locus_tag="PK049_10645"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HOK62059.1"
                     /translation="MILLSGNGTIIISAIAVFLLIILLLVAVLLYAKTKLTPQGRVRL
                     VINEEKTLEVDPGGTILSTLSNNGVFLPSACGGGGTCGMCKCRVLEGGGSILPTEVGF
                     FTRKQQNDHWRLGCQVKIREDIKIEVPEEVLGIKKWECEVVSNHNVATFIKEFVVKLP
                     EGEKLHFRSGGYIQIDVPKIEVDFAKDIDVEPEYRDEWDRYKMWDLKMKNTEETFRAY
                     SMANHPAEDNIIMLNVRIATPPWDREKNAFMNVNPGVCSSFIFSRKPGDKVMISGPYG
                     EFFIKDTQNEMMFIGGGAGMAPMRSHIFDQFLTKRTTRKATFWYGARSKREIFYEEHF
                     RKIEQEFPNFKFTIALSEPKPEDNWTGYVGFIHQVIYDNYLSKHDAPEDIEYYLCGPP
                     AMNAAVQKMLFDLGVPEEMIAFDDFGG"
BASE COUNT         1851 a         1286 c         1592 g         2098 t
ORIGIN      
        1 taaccgttag ttccatttgc tggtgggtgc tttgaatact tggttcaatg ggttgacgaa
       61 taagggcatc aacttttacc agtggctcat ataccctaaa ctgttgttga agaacaattt
      121 cgctcctatt gctttgcctt acaacttgca cgatgtaatt acctgaaagt ttaagcttaa
      181 ggttactatt tggtattgta agggtatagt gcctatattg caccaacgtt ccggaagaat
      241 accgataatc gtgaatgggg ttaaagtcaa aaccatcggc atactccatg aacaccatat
      301 ttgtagggtt ccagctatag tcgcaatgaa ttacagtgta ttcgtattgc tccgtttcat
      361 ctgataagtc gtcgaattcc aaagttatag tttcgtcggt attcaactct attattggtt
      421 gagttagctc aaaaccggtt ttgtaggctt taaccgattt tatgtttgtt ttccattggt
      481 tcgataattt ttcaggtttt ccaaaaacat tttcagccac ttctctgtta tttgagtaac
      541 aaagcagagg gcttaatatg tataagataa aaatagatat attaatatct atatatctgg
      601 taaatttgat atacatcaat aaatattttt gttaacgtgt tatcaaagat actacatttg
      661 tggctgagtt tttttaaaca ggagttcaaa attccttaaa tttgttttag caaaacacta
      721 aaataatgtc taaaccaatt aaaatcaaac gtggattgaa tatcccgtta aagggttcag
      781 ctgagaaggt gctggttcgc ctcgataagg ctgaaactta cggggttaag ccaaccgact
      841 ttcccgggct tgtacccaag ctcaatgttg cggttggcga taaggtcagg gcgggttcgc
      901 cattgtttca cgacaagtat aggcctaaca tagtattttc atcgcctgtt agcggtgaag
      961 tggttgatgt taggcgtggt gaacgcaggg taatactgga ggtagttgta aaggccgata
     1021 gtggcgaggt ggcttatgag gactttggtc ctgcatcgcc cgaaacgctt acccgcgatg
     1081 aggttattgc aaagttgctc aaggcaggag cttggcctta cattaggcaa aggccttttg
     1141 gcattattgc caatcctgct gattccccaa aatcgatttt catttctggc tttgatacct
     1201 caccgctggc tcccgattta gattttgtag ttcagggtga ggaggagagt tttcaggcag
     1261 gcgtggatgc gattcgtaag ctaaccaccg ggaaggttca cttggggtta agttccgatt
     1321 ttccacccgc aaaagctttt gccaatgcaa aaggagtgga gttccactat tttactggac
     1381 ctcatcctac tggtaatgtt ggtgttcaaa ttcaccatat cgacccaata aataaaggag
     1441 aggtagtatg ggtagtaaat cctctggatg ttattattat tggacgcctt ttccttaaag
     1501 gtatctacga tgcaaccaag gtaattgcct tagctggctc cgaggttgtg aaaccccgat
     1561 actataagtt gatttccggt gcaagcattg agagtattat taaggataat attaatacac
     1621 agggtcaagt tcgtatcata agtggaaatg tgcttaccgg tacacgtgtc gataaattgg
     1681 gatatttagg tttttatgat aacatggtaa ccgtaatacc cgaagggaat aagtatgagt
     1741 ttttcggatg gattgctccg ggtgtcaata agcatagcgc ttcaagtacc tttttatcga
     1801 aattaatccc aggcaagagg tttaccctcg ataccaatat gcatggtggt catagggcaa
     1861 tggtggttac cggccagtat gagaaggtta tgccaatgga tatttaccca atgcatctag
     1921 ttaaagccat tctggctaat gatatcgata ggatggagca actaggaatt tacgaagttg
     1981 ttgaggaaga tatggcgctt tgcgagtatg tgtgcccttc aaaaactgag gtgcaggcta
     2041 ttctacgcaa gggcattgaa accatgataa aagagttaag ctaacaataa tatcattgta
     2101 atgaaagcgt tacgaaatta cctggataag attaagccgc actttcagaa aggtgggagg
     2161 tttgagatgt ttcactcaac ctttgatgcg tttgaaactt ttctgtttgt gcccaataca
     2221 gttactacca agggaagcca tattcgcgat tcgctggact tgaagcgcac tatgtccatt
     2281 gtgattatcg cattaatgcc tgccatgctt tttggaatgt ggaatgttgg ttaccagcat
     2341 gctctttcca ttggtgcaag ttggggcttt ttagagacat tctggtatgg ttttcttaaa
     2401 gttttaccat taattgtggt aagctatgga gtgggacttt ccattgagtt tgcatttgcc
     2461 cagtccagag gccatgaggt taatgaggga tttctagtta gcggcttgct tatcccgcta
     2521 atcatgcctg ttgacgttcc tctctggatg cttgccattg cggttgcttt tgcagtaata
     2581 attggtaagg aggtttttgg gggaacaggt tacaacgtat tcaacccggc tttgctggca
     2641 agggcgttcc ttttctttgc ctacccttcg cacatgtcgg gcgataaggt ttggattgcc
     2701 ggactgactc aggggaaagg agtagtggat ggtttttcag gagccacccc tcttgccaaa
     2761 gcagcagcct accaaattga ccagttgcct tcagtatacg atatgttcat gggctttatt
     2821 cctggctcag ttggtgaaac atctacttta gcaattctta ttggtgctat catacttata
     2881 gtaacaggaa ttggcagttg gaaaatcatg gtttcggtat ttggaggtgg cttggcagca
     2941 ggtttacttt ttaatgcctt tgcggttaac ccctacatgg agattccagc atggcagcac
     3001 ctaattatgg gaggctttgc atttggtgct gtttttatgg ctaccgaccc cgtgagcgca
     3061 tcgcaaaccg agaagggtaa gtggatatac ggatttttgg taggttttct ggccctgctt
     3121 gtcagagtgc ttaaccccgc ttacccggaa ggtatgatgc ttgccatcct gcttatgaat
     3181 gcatttgctc cactaattga ttactacgtg gttgaagcca acattcgccg aagaatgaaa
     3241 cgcgccaaaa aggttgctaa cgtctaaact ttgaaagcta tgagtttact ggataagata
     3301 aaaagcatgg accgaaacag caatgcctat acaattatgt attcttcggt aatggttatt
     3361 gttgttgcca tgctgctggc tattgcctca acggctttaa aaccggcaca aaccaagaat
     3421 attgaaacgg aaaagaaaat cgatatactt cgctcaatag gcaaggccga tggggttgaa
     3481 caagccgcca acaagcatgc atttgtttct gaaaacttca ataagtatat ccctgagcag
     3541 gttgttgtta actcaaaagg cgacaccata accggtgtag ttgcatttaa tgtagatttg
     3601 aaggttgaaa tgtccaagcc cattgagcag cgaaaccttc cggtttacaa ggctaaactc
     3661 gatgatggta gcataaagta tatcattccc cttcgtggac gtggcctttg ggggcctatt
     3721 tggggctata tcgctttaaa cgatgatttg aataccattt acggtgccac ttttgcccat
     3781 cagggcgaaa cgccaggatt aggcgctgaa atctccactc acgaatttca ggagcaattc
     3841 aagggaaaat caatttttca gggcgacagc attttaacct ttacttccgt aaaggtggta
     3901 aaaggtggtg ccgcacccaa cgatccccat ggggttgatg ccatttcggg tggtaccatt
     3961 accagcaaag ggctcgaaag tatgcttttc gattgccttg ctgcctacca aagctatttt
     4021 caaaaagtaa aaaacagtat tagccatgag tgaagttaag gaaagggaac catggttctc
     4081 cccaaagaac attaagctgc ttaccgagcc aattgactct aaaaacccag tgactgtttt
     4141 agtattgggt atttgttcag ccttggctgt taccgtaaag cttaagcctg ccattgtgat
     4201 ggccatttcc gttacagtag taaccgcttt ctcaaacctg ataatttctt taatccggaa
     4261 ttcgattcct aaccgtatcc gtattattgt gcagctggtt gttattgcag cgctggtaat
     4321 tctggtcgac caaatactga aagcttatgt ttacgatgta agcaaacagc tatcggtttt
     4381 tgtagggtta attataacaa actgcataat catgggacgt ctagaggctt ttgcactagg
     4441 taataagccc tggccatcgt ttctcgatgg tattggtaac ggattaggtt atggtttaat
     4501 cctggttatt gtagcttttt tccgtgagct acttggctca ggaaccattt ttaactatag
     4561 ggttataccc attgattggt atatcaaaaa cggaggtttt tatgagaata atggtatgat
     4621 gattctacct ccgatggcgc tgattactgt tggtatcata atttggatac aacgtagcag
     4681 gaaccgaaaa cttattgagg attaaccaca aaaaattgac ctaaggtatg gaacatctaa
     4741 tcaacatatt tgtcaagtcc atatttgtcg ataatatggt ttttgcttac ttcctaggta
     4801 tgtgctcgta cctggccgta tcaaaaacag taaagacgtc aatggggctt ggcattgcag
     4861 taatattcgt gatggctatt actgttcccg taaactattt aatgaacaag tacttactac
     4921 taccgggtgc gttaagctgg cttgatcccg gttttgcgaa tttagatcta agcttcctct
     4981 cgtttattat gttcattgct gttatagcat ccatggtgca actagttgag atggtggtag
     5041 agaagttttc gccatcgtta tacaactccc ttggaatatt tttacccctt attgcggtaa
     5101 actgtgcaat actgggaggc tcattattca tgcaggaacg tgaatacgaa acccttgccg
     5161 aggctactgt ttttggggtt ggatcaggaa taggttgggc tctagcaatt gttagcattg
     5221 ctgcaatccg cgaaaaactc aaatattccg atattcctaa accattacgc gggttaggta
     5281 ttgcattcat tcttacaggt ttaatgggca ttgcgttcat gacctttatg ggcatcaagt
     5341 tgtaaaaact gtaaaactat ttaaccatga tattactttc agggaacgga accattataa
     5401 tttcagcaat tgcagtattc ctgctaatta ttttgttgct ggtggcggtt ctgctatacg
     5461 ctaagacaaa gcttaccccc cagggtaggg tgaggttggt aattaatgag gagaagactc
     5521 ttgaagtaga tcccggggga acaattcttt caaccctatc aaataacgga gtttttctgc
     5581 catccgcatg tgggggaggg ggaacttgtg gtatgtgtaa gtgcagggtt ttggaaggtg
     5641 gtggcagtat tttaccaacc gaggtgggat tttttactcg taagcagcag aacgatcact
     5701 ggcgactggg ttgtcaggta aaaattagag aggacataaa gattgaggtg cccgaggagg
     5761 ttttaggtat taagaagtgg gagtgcgaag tggtatcgaa ccacaacgtg gcaactttta
     5821 ttaaggagtt tgtggttaag ctacccgagg gtgaaaagtt acatttccgt tccggtggct
     5881 atatccagat tgatgtacct aagattgaag tagattttgc taaggatatc gatgttgaac
     5941 ctgaataccg cgacgagtgg gataggtaca agatgtggga tttgaagatg aagaataccg
     6001 aggaaacatt ccgcgcatac tccatggcaa accatccagc ggaggacaat ataatcatgc
     6061 tcaatgttcg tattgctact ccgccctggg atagggagaa aaatgctttt atgaacgtaa
     6121 accccggggt ttgttcaagt ttcatttttt cgcgtaaacc cggcgataag gttatgattt
     6181 caggacccta tggcgagttc ttcataaagg acacccagaa tgagatgatg tttattggtg
     6241 gtggtgctgg aatggctccc atgcgctcgc atatatttga tcaattccta accaaacgta
     6301 ctacccggaa ggctactttc tggtacggag cccgttcaaa acgagaaatt ttctacgagg
     6361 agcacttccg taaaattgag caggaattcc ctaacttcaa attcaccatt gccctttcag
     6421 aacccaaacc agaggataac tggactggct atgtaggatt tatccatcag gttatatacg
     6481 ataattacct ttcaaaacac gatgctcctg aggacattga gtactacctc tgtggtccac
     6541 cagccatgaa tgctgccgtt caaaaaatgt tattcgattt aggcgtaccc gaggagatga
     6601 ttgcatttga cgattttggt ggataaaaca ttgtttaact aaacttaata ttcctttaac
     6661 ccgcattagc taatgcgggt tttatctttg cagtgtcatt tgaaacagaa aagagttagt
     6721 tttctcatag gcttttggtt tttgagttag gtttaggttt ggttttaggt taaaggtaac
     6781 aaaatggggc tgtaaagccc cttttgtttt tatattgata tttcaat
//
LOCUS       DAOSAL010000102         6792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_7204_length_6792_cov_49.278908, whole genome shotgun sequence.
ACCESSION   DAOSAL010000102 DAOSAL010000000
VERSION     DAOSAL010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 6792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6792
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7204_length_6792_cov_49.278908"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1470)
                     /locus_tag="PK049_10650"
     CDS             complement(<1..1470)
                     /locus_tag="PK049_10650"
                     /inference="COORDINATES: protein motif:HMM:NF021379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HOK62060.1"
                     /translation="MHRNRKQIYWFVVILTVFFIVFCSISLVNHYLFRTYALDLGMFN
                     QALRNVAQLQKPTFTLGIAGEEIPFLATHFSPIIFLFTPLCFIFGNYTLLIVQIVAIL
                     FGGAGIFLYSKHRTNSDNVTPIVSLIHFLSIWGIYSALTYDFHSNVLGAMLVPWLFLF
                     YERKQYKHAFIVVILAILTFETMTIWFAFIIAALAISSYNRQKLVQVDIPLFAFCVLS
                     AIIIIWVAMPLLQSTSTNMQFERYRWLGETPMQVVLNLILSPINLFKVLFTNTTSDVA
                     YDYIKLEFHIIVLVSGGILFLLKPRFLFMLIPIYMLKMIPNDYNFWGINNQYSIEFVP
                     ILSIAAISTISEIKINYRLPFAIALAIVTAAATAFTMENRVSKWYDGINTRFYNKQHY
                     QTEFDIKRINEAIRITDGSKAVSASSRLAPHLNARKIYSYPFVKDAEYILLLPTSNTW
                     PLSADDFKKSIDTLSNSQHFTKVYSDNDLLIFKRNERKAI"
     gene            complement(1557..3665)
                     /locus_tag="PK049_10655"
     CDS             complement(1557..3665)
                     /locus_tag="PK049_10655"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOK62061.1"
                     /translation="MKRIVKPICLAAMAIIIGGSGCTDTEKQVKEPLKIDVSLTQEEK
                     DKGLLTPEIMWKFGRIGSSVISPDGKLLAYTTTYYNLQENKGVTNIYLIPTEGGEPVM
                     LTDEQGSESNIQWSADGQAIRFLSTRSGSSQIWEVKIDGTGLKQLTNFDFDINGFAFS
                     PDGKKIVYAKDVKMVDQTEDIHPDMPKAKVRIIDDLMYRHWDYWEDGKFSHLFVADYT
                     ENGVENAIDINEGEKWDTPMATDFDMDEVQWSPSGDKIVYASKKLYGKDYAMSTNSDI
                     YLYDLKTRITTNLSDGNPGYDRYPRFSPNGVFISWWSMATPLYESDQKRLFILNTNNN
                     KKTYLTEGFDQNVEYYVWDNDSRNIYFTSGIKGTEQLYKVDVATSKITQLTTGKHDYT
                     SCQYSNGVLTASRMSMSMATELYRINEQTGEATQLTFTNKHIYDHIKMGEVRERWVTT
                     TDGKKMLVWVILPPDFDSTKKYPALLYCQGGPQSTVSQFWSYRWNFQIMAANGYVVVA
                     PNRRGVPSFGQEWNRQISGDYSGQNIKDYLSAIDDVKKEPWVNEDKLGCVGASYGGYS
                     VFYLAGHHQKRFKAFIAHCGMFNLESFYPQTEELFFPTFDLGGAPWETDNKVAQRSYA
                     NSPHKFVKNWDTPIMIIVGEHDYRIPYTQSLEAFGAAQLLGVPSKLLFFPNGTHFVTK
                     PQEAVVWQREFFGWLDKWLK"
     gene            3815..5428
                     /locus_tag="PK049_10660"
     CDS             3815..5428
                     /locus_tag="PK049_10660"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOK62062.1"
                     /translation="MRKLFPLVLLLSVLSACNSSNKLNTNAISYDDVEHDIMVLSADS
                     MMGRAPFTVGEVRATAYIQNRMKDLGLVPAFGDTYFQDVPMVSITTHLPEQVDVGLAS
                     GKLSLKVDDDICLWSPTTRAKVEISKAPLLFLGFGINAPEYGCNDFRDIDVKGKIIVV
                     LVNDPGYYLLDSSLFTGYAMTYYGRWRYKFEEAERQGALGCIIVHEDGPAGYPWAVAG
                     SKSNSPILYVDSKELDTPACTLNGWITREAAEQLFSRCGYSYEAIKKQATSKDFKPIN
                     LNATLSLSLSNSVERSVSKNVAGYIKGAEKPDEFIVYTAHWDHLGVGRPLNGDSIYNG
                     ASDNAAAIAWMLSIAKVFKQADAPKRSIIFLAPTAEESGLFGSEYFVKHCPFDITKAI
                     ACINYDVVLFLGKFKDVTVTGYGHSNLDSLLQVVAEEHDRYIALDPNPENGMFYRSDQ
                     LPFLKAGVPAIFAKGYTHQAELGKEKTLELIKDYWQNVYHKPCDEYNPQRDRLDGLVD
                     DAVLFYEVGNKLTNSNIYPQWRKASEFYR"
     gene            6026..>6792
                     /locus_tag="PK049_10665"
     CDS             6026..>6792
                     /locus_tag="PK049_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK62063.1"
                     /translation="MDFNSFIKSLSEISERRLMYALIAVILFIDMTILVLSNGYSGGS
                     YSVMHYQFARWAFVNPENLLNSWANPIYTLLAAPFALLGFKSLQLMNIMLGILSGYFA
                     YRVAKELKMKTPLLALVICSFTPVFMMTFFGGTTEIMFAFVAILGTFLLIKDKFFAGA
                     VVVSMLPLIRLDGFILLPVYAIYFLRWHRVKYLPLLLSATVLYSLIGLMFGKSLFWLF
                     EGVKLWSDSIYGHGSFTQFIVRSPGFFGIPNEIFFVTG"
BASE COUNT         1815 a         1424 c         1489 g         2064 t
ORIGIN      
        1 aatagcctta cgctcgtttc ttttgaatat cagtaaatcg ttgtcggaat aaaccttagt
       61 aaaatgctgg ctattgctta aagtatcgat tgatttttta aagtcatcgg cgcttaatgg
      121 ccatgtgttg cttgtcggta gcagcaaaat atattcagca tcctttacaa aagggtagga
      181 gtatattttg cgggcattaa gatgtggtgc aagccttgaa cttgccgata cggcttttga
      241 accatcggta atcctaatag cttcattaat tctttttatg tcgaactcgg tttggtagtg
      301 ttgtttatta tagaaacgtg tgttaatccc atcgtaccac tttgaaactc tattttccat
      361 tgtaaaagct gtggccgctg ctgtaacaat tgcaagggca attgcaaagg gtaaccgata
      421 atttatctta atctccgata ttgtagaaat agcggctata cttagtattg gaacaaactc
      481 aatggaatac tggttgttga ttccccaaaa gttgtagtca ttagggatca tctttagcat
      541 gtaaatgggg atgagcatga aaaggaagcg tggttttaaa aggaaaagta ttccacctga
      601 caccaaaaca ataatatgaa attccaactt gatgtaatcg taagccacgt cgcttgttgt
      661 gttagtgaaa agaactttaa agagattgat aggtgaaaga atcaggttta aaactacttg
      721 cattggcgtt tctccaagcc atcggtatct ttcgaattgc atgttggtgc tagtgctttg
      781 cagcaatggc atggctaccc aaataataat tattgccgat agaacacaaa atgcaaaaag
      841 tggaatatca acctgaacta acttttgtcg gttgtagctg cttatggcaa gagcggcaat
      901 aataaaagca aaccatatgg tcatggtctc aaaggttaaa atagccagaa ttacaactat
      961 aaatgcatgc ttgtactgtt ttcgttcata aaataggaat agccatggaa ccagcattgc
     1021 gcctaaaaca ttgctatgaa aatcatacgt aagtgcggaa taaatacccc aaatgcttaa
     1081 aaagtgaata agcgatacta ttggtgtaac gttatcgctg ttcgttcggt gtttggagta
     1141 taggaatata ccagctcccc caaaaagaat tgcaacaatt tgcactatca gtaaggtata
     1201 atttccgaag ataaagcata ggggagtaaa cagaaaaata attggagaga agtgcgttgc
     1261 aaggaaaggg atttcctctc ctgctatacc aagagtaaat gtaggttttt gaagttgtgc
     1321 aacattcctt aaggcctgat taaacatgcc caaatccaga gcgtaggttc ggaataggta
     1381 atggttaact aacgatattg agcaaaatac gataaagaat accgtgagaa ttactacaaa
     1441 ccaataaatt tgtttcctgt tccggtgcat ttcaaatatt tacatgatgt aaatataaaa
     1501 aaaaacggga gtaaccctta aggaactccc gttttattat ggatttgatt cagtatttac
     1561 tttaaccact tatcgagcca gccaaaaaac tcgcgttgcc aaacaacggc ttcctgtggt
     1621 tttgtaacaa aatgggtgcc gttggggaag aatagcagtt tacttggtac gccgagtagt
     1681 tgcgcagccc caaatgcttc gaggctttgg gtgtaaggta tacggtaatc atgctcaccc
     1741 actataatca taataggagt atcccagttt ttaacaaact tatggggcga gttggcgtag
     1801 cttcgctgtg ctactttgtt atcggtttcc catggcgcgc cgccaaggtc gaaggtgggg
     1861 aagaatagtt cctcggtttg ggggtagaac gattccaggt taaacatgcc gcagtgggca
     1921 ataaacgctt taaagcgttt ctggtggtgt ccggcaaggt agaacactga gtaaccaccg
     1981 tagcttgcgc ctacacaacc cagcttatcc tcattaaccc aaggttcttt cttaacatca
     2041 tcaatggcgc ttaggtaatc cttaatgttt tgtccgctgt aatcgcctga gatttggcgg
     2101 ttccattcct gcccaaacga tggaacacca cggcggttag gagccaccac aacgtaacca
     2161 ttggctgcca taatctggaa gttccagcga tagctccaga actggcttac ggtgctctgc
     2221 gggccaccct ggcagtaaag tagggcggga tacttctttg tggagtcgaa atcgggagga
     2281 aggattaccc aaacaagcat ctttttccca tcggttgtgg taacccagcg ttcacgaact
     2341 tcgcccatct taatatggtc gtaaatatgc ttattggtaa aggttagctg agtggcctcg
     2401 ccggtttgct cattaatccg gtaaagctcg gtagccatgc tcatgctcat gcggcttgca
     2461 gtaagtacgc cgttggagta ctggcagctg gtgtagtcgt gcttgccagt agtgagctgg
     2521 gtaatttttg atgtggcaac atcaaccttg tagagctgct cagtaccctt aatcccacta
     2581 gtaaagtaaa tatttcggct atcgttgtcc caaacgtagt actcaacatt ctggtcaaaa
     2641 ccctcggtaa gataggtttt cttgttgtta ttggtgttga gaataaaaag tcttttctga
     2701 tccgattcgt aaagaggggt ggccatactc caccagctga tgaagactcc gttaggagag
     2761 aatcgtgggt agcggtcgta accgggattg ccatcggata ggtttgtggt gattcgggtt
     2821 tttagatcgt acaggtagat atcggagttg gtgctcatgg cgtagtcctt cccataaagt
     2881 ttctttgagg cgtaaacaat cttatcgccg cttgggctcc actgcacttc atccatgtcg
     2941 aaatcggtag ccatgggggt atcccacttt tcaccctcgt tgatatcaat ggcattttct
     3001 accccatttt cagtgtaatc ggcaacaaac aggtggctaa acttgccatc ttcccagtaa
     3061 tcccagtggc gatacatcaa atcatcaatt atccgtacct tggcctttgg catgtccgga
     3121 tgtatatcct cggtttggtc aaccatcttc acatccttgg cgtacactat ttttttgcca
     3181 tcgggcgaaa aggcaaagcc gttaatatca aaatcgaagt tagtgagctg tttgaggccg
     3241 gttccatcga tttttacttc ccaaatttgc gaggaaccac tacgggtgga caggaagcgt
     3301 atggcttggc catcggcact ccactgaata ttcgattccg agccttgctc atcggtcagc
     3361 attactggct ctccaccctc ggtaggaatt aggtagatat ttgttactcc cttattctcc
     3421 tgtaggttgt agtaggttgt ggtgtaggca agtagtttgc catcgggcga gatgaccgaa
     3481 ctcccaatac gtccgaattt ccacatgatc tcaggtgtga gaaggccctt gtccttttcc
     3541 tcctgggtta acgatacgtc aatctttaga ggttccttaa cttgtttttc ggtatcggta
     3601 cagcccgaac cgccaataat tattgccatt gcagcaagac agattggttt tacaattcgt
     3661 ttcatggtgc tttgggttag gttacacaat tttgacccta aagttagcat ttggttaaat
     3721 atcaacctat gatttttact ttacttcttt ttttagtcaa attaaaagac tagggataaa
     3781 ttaattttta cttttgttca aaactactaa tgccatgcga aaactatttc ctttggtact
     3841 gcttctatct gtcctatctg cctgcaatag cagcaataag ctgaacacaa atgccatatc
     3901 ctacgacgat gtggaacacg atataatggt gctttcggcc gatagcatga tgggacgtgc
     3961 accatttacc gtgggcgaag taagagcgac cgcatacatt caaaacagga tgaaggactt
     4021 agggcttgtg cccgcttttg gtgatacata ttttcaggat gttcccatgg tttccattac
     4081 aactcattta cccgaacagg ttgatgtagg tttggcaagc ggtaagctta gccttaaggt
     4141 tgatgacgat atctgcctgt ggagtccaac cactcgggca aaggttgaaa tatccaaagc
     4201 acccttgcta tttttgggtt ttggaataaa cgcaccagag tatggctgta acgatttcag
     4261 ggatatagat gtaaagggca agatcattgt ggttttggtg aacgaccccg gttactacct
     4321 gcttgactca tcgcttttta ctggctatgc catgacctat tacggacgct ggcgctataa
     4381 gtttgaggaa gccgaacgcc aaggagcgct tggctgcata attgttcatg aggatggtcc
     4441 tgcaggttac ccatgggcgg ttgcgggtag caagagcaac agcccaatac tttacgttga
     4501 tagtaaagaa ctcgatactc cggcgtgcac tcttaatggc tggataaccc gtgaggccgc
     4561 cgagcaacta ttcagtcgct gtggctatag ctacgaggcg ataaagaaac aggctaccag
     4621 taaggatttt aaacctataa acctaaacgc aacattgtct ctaagcttaa gtaatagtgt
     4681 agagcgttca gtatcaaaga atgtcgcagg ttacattaag ggggctgaga aacccgatga
     4741 gtttattgta tataccgctc actgggatca ccttggggtt gggcgcccac taaatggcga
     4801 ttccatctac aatggagcat cggataatgc tgccgcaata gcatggatgc tatccattgc
     4861 taaggtattt aagcaagccg atgcacccaa acgctcaatt atcttccttg cgcctaccgc
     4921 tgaggagtcg ggactgtttg gctcggagta ctttgttaag cattgcccgt tcgatataac
     4981 aaaggctata gcctgcataa actacgatgt ggttcttttc ctcggaaagt tcaaggatgt
     5041 aaccgtaacc ggttacggac actcgaatct cgatagtctt ttacaggtgg ttgcagagga
     5101 acacgaccgc tacattgccc ttgacccaaa tcccgaaaat ggaatgtttt accggtccga
     5161 ccagttacca tttcttaaag ccggtgttcc tgcaattttt gctaaagggt atacgcatca
     5221 agctgagttg gggaaagaaa aaactcttga gctaattaag gattactggc aaaacgttta
     5281 ccataaacct tgcgacgagt ataatccgca gcgcgatagg ttagatggtt tggttgacga
     5341 tgcggttctg ttctacgaag ttggtaacaa gttgacgaat agtaatatct atccgcaatg
     5401 gcgcaaagca tcggagtttt acaggtaacc tgccgacgtt taatcgagat tcgtcaattg
     5461 aattttcaat tgacgaatcc ctctaaaaat attgctgcgt tccaattttt ttgcccggtt
     5521 aagttagtta atctatggta tttagaatta actgctcaca ttacctctaa cttcttctaa
     5581 catcatctta ttccgtctat tccgactcat tccggcacaa aacagacttt taacttttaa
     5641 cttttaacgg ttcacggttc acgaacataa agctctccaa ccccctcata tcctcaattc
     5701 ttcttttaac ttttaacttt tcactagtcc taactccctc taacttcctc taacttcctc
     5761 taactccgtc atattcttaa ttcttccggt tcacggttca cggttcacgt ttaacttcct
     5821 ctaacttcct ctaacttcct catattccag cttattctgg ctcattccga ctcatacctc
     5881 aagaaattaa cccggttgaa tttgctagtt cattgcattt gggtttaaat attaatgtta
     5941 tttttatgcc gggattcgat gtttcaattc gcgttttcaa tttttactat ttgtttaaac
     6001 aacagtaact aaaccttata ctaggatgga ctttaacagt ttcattaaat ccttgagcga
     6061 aatcagcgag aggcggttga tgtatgcgct tattgcggtc atacttttta tcgacatgac
     6121 aatacttgta ctaagcaatg gctattccgg cggttcttac tccgttatgc attaccagtt
     6181 tgcccgttgg gcatttgtta atcctgagaa tcttttgaat tcctgggcaa atcccattta
     6241 tacattactt gctgctcctt ttgctctgct tggttttaaa tcgctgcagc tcatgaacat
     6301 catgctggga atactatcgg gttactttgc ctacagggtt gccaaggagt tgaaaatgaa
     6361 aaccccgctg ctggctttgg ttatctgcag ctttaccccc gtttttatga tgactttctt
     6421 tgggggcaca accgaaatta tgtttgcctt tgtggccata ctgggcacat tccttcttat
     6481 caaggataaa ttttttgctg gtgcagtggt tgtatcaatg ctacccctaa tccgtcttga
     6541 cggttttatc ctgttgccag tttacgcaat ctatttttta cgctggcaca gggttaagta
     6601 tttgcccctt ctactaagtg caacggtgtt atacagttta ataggattga tgtttggtaa
     6661 gagccttttc tggctttttg aaggggtaaa gctatggagc gatagtattt acgggcatgg
     6721 ctcatttact cagtttattg ttcgtagccc aggatttttt gggataccca acgagatatt
     6781 ctttgtaacc gg
//
LOCUS       DAOSAL010000103         6578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_7490_length_6578_cov_52.880730, whole genome shotgun sequence.
ACCESSION   DAOSAL010000103 DAOSAL010000000
VERSION     DAOSAL010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 6578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6578
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7490_length_6578_cov_52.880730"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1283)
                     /locus_tag="PK049_10670"
     CDS             complement(<1..1283)
                     /locus_tag="PK049_10670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOK62064.1"
                     /translation="MPYKVLTACGNIQLLEDIQRIFSLADDDFTVSNILDSERIISTI
                     TNNNPDIILYDIHFANKAAADVLSIIKADPATSNIPILAIADFLQPEHIQSLFHAGAD
                     DFIGLPINTPELIQRTMVGIQRGRMLKKLKTLSDQFDSITTAISQAGNSVIIISNTGE
                     ITWVNEGFKRLYECSVEEFKATFGQNLFSETINKTTAEAIRKCIVGEYVVYESKWVTP
                     MGKEKYIQTSLTPVFDDLQNLSYIVAIEMDITDLKLIEKDLAEKNEHLQSIMENLENA
                     NKILEEQQNQIQKQSELLAEEKQKTEALLLNILPLEVANALQKKGIYKPKKFKEVSVM
                     FADFVGFSKISVAYENIDEFLNVLSSYFEAFDEIVTQRYIEKIKTIGDAYMCVGGLPR
                     TNHSHPFDTVLAALELQRYTKARNIEEAQANKPTWS"
     gene            complement(1384..1593)
                     /gene="xseB"
                     /locus_tag="PK049_10675"
     CDS             complement(1384..1593)
                     /gene="xseB"
                     /locus_tag="PK049_10675"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOK62065.1"
                     /translation="MTKKPVKYSEAIEEVERIINQIESNELDIDELTEKIRRASELLK
                     FCKQKLHFTEEEIQKIIDQMQKDDE"
     gene            complement(1574..4459)
                     /locus_tag="PK049_10680"
     CDS             complement(1574..4459)
                     /locus_tag="PK049_10680"
                     /inference="COORDINATES: protein motif:HMM:NF026288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOK62066.1"
                     /translation="MLQKIKVPLFLALLAFRSFSQGSKVNIDSLIATIKKYSIENPEV
                     SAISCNRLFLQVKQNPSDSLRALYHLAMSEYKSGLGLTGDALGHVDSAITITQSKRTQ
                     SLYVDALLKKSALLQRSGNYSEAIKVNEKARLTALKGKNNRLLIQSLLLRGDIYKEIG
                     QYNDALSKKGEALAIAKKIGVGIAECHQSIGSTQWNFGNYQEALKSYFEGLIAAEKDN
                     DTLRIIQLLKNIGLTYRETGNFDKSTSHLSKALQIAEKSKLNDELADILNIMGGVYLK
                     FSQPAKAIEYYKQSLNIRGTLGYKQSSINTLNNLAKAYLLLKNSSESITSLNKALELS
                     KEIGDPLTEANIHNELGNTYLTIEDYPEALRRYLLALKIRQSYGKDEDIARTMVNIAL
                     VYRRLGLFRNAQKYMSEAKGLVENLDAKPDLKIYIYQNMGNVYYDQKQYTNAINAYQK
                     ALELSEKKGDDLQVTRVLRNIAQTYLDLNKPSKARNTITQAIKIALRSNQNQLLADLY
                     NTLGNIERQDNNFDLALTHYNTAKEKYKQNLNADGEALCLRKIGEIYSLQGRYRDAKV
                     NITQSIRIGKSVGNPYLVLLGTLALSDVEKAQGKFQNALTLYQKYVYLKDSIEDAKRS
                     EANIDAHINLELDKTKTEIKLIEAEVEALRTKVELDRERINKQKAQRNLLVTAVIFLL
                     FIAFGSFYLFNQKKNLARLQREKYEEEERINQKLKQYQAELEQNIKTKDKLFSIVAHD
                     LRSPFTALVGLSEILATKADELPPSEVKEFSNHIHQSASNVLALTENLLSWARSQTGK
                     LVLNQAYINICELISKAVEVASLPAGEKEIKIEVICNETVTAFADYDTLLTIIRNLLS
                     NAIKFTPGGGKISLKASSDNQKVTIQVTDTGVGIAPENLSNLFRLDGFTTKGTNSESG
                     TGLGLMLCKEFAEANGGTISVSSTLGKGTTFTITLPAKNVMYDQETC"
     gene            complement(4531..>6578)
                     /locus_tag="PK049_10685"
     CDS             complement(4531..>6578)
                     /locus_tag="PK049_10685"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing sensor
                     histidine kinase"
                     /protein_id="HOK62067.1"
                     /translation="LIDISKYYWDVNPDSSVIFAEKSLKEAKADGSDDAIGDAYNALG
                     NAYSSKYQNETALNYYLKSLEFRKLNGNPLKIGNSYHNLGLVYRELKQFNLAVSSHTK
                     AADYYEKAKRYDLQSISYNRIASLYNQINDKNKALEYAFKALNIVNENNIDELKANLY
                     NLIGSLNRDLKNLELAEKYYTLAFKEWEKSKNISGLSTAYNNLGIVLDEKGDLQKALE
                     YYTKSLEFATQQRDSSGIATAYNNIGFLYARIKQFDKALMSYQKSYEISVKTNGVDAA
                     HNTLNNIASTYLKQNNLEKASEALNKALRNIPNVVDQAYKQETYQLLSDLYAKKGDYR
                     NAYHYKCVELAYADTLYTQQRLSSIAEMQARYETETKEKEIELLKKDVELKELQFQKQ
                     RSVQQVLISLTLLLLAIFFGTSLILTIIRKKNKQLAEKNAELFEINKKLKESEKNLSE
                     LNATKDKLFSIIAHDLKNPFNALIGFSDILERNFNHLTDNEKKEYISVISESAQNLYK
                     LLDNLLQWTRAQTGSINYIPEKFKLEPLIKQEVLNLNANAEKKRINVSVNASSAISVY
                     ADKNSIATVIRNLLSNAIKFTDIGGTIEIIASESKDFPKMAEVVVKDSGIGIDQDDLE
                     RIFMIDGSYSTKGTANETGTGLGLLLCHEFVTKNNGKIWVNSSKGSGSEFHFTIPLA"
BASE COUNT         1603 a         1290 c         1373 g         2312 t
ORIGIN      
        1 gaccaggtag gcttattggc ctgcgcctcc tcaatgttcc ttgctttagt atagcgctgg
       61 agttcaagtg ctgcaagcac agtgtcgaac gggtggctat ggttggttct tggcaatcct
      121 cctacgcaca tgtatgcatc gcctatcgtt ttaatcttct caatatagcg ctgggtaact
      181 atttcgtcga atgcttcaaa gtagctgctc aggacattaa ggaactcatc aatattctcg
      241 tatgccacac ttatttttga gaatccaaca aagtcggcaa acataaccga aacttccttg
      301 aactttttag gcttatatat cccttttttc tggagggcgt tggcaacctc aagtggtaaa
      361 atatttagca gcaaggcctc ggttttttgt ttttcctctg caagcaattc ggactgtttc
      421 tgaatttggt tttgttgttc ctctaggatt ttgttggcat tctcaaggtt ctccattatg
      481 ctttggagat gttcattctt ttcggctagg tctttctcta ttaacttaag gtcggtaata
      541 tccatttcaa tggctacaat gtagctcagg ttctgcaagt catcaaaaac aggtgttaga
      601 ctggtttgaa tatatttctc cttgcccatg ggagtgaccc atttactttc gtaaaccaca
      661 tattccccaa ctatacattt tctaatagct tcagcagttg ttttatttat ggtttcggag
      721 aaaaggtttt ggccaaaggt tgctttaaac tcctcaacac tacattcgta aaggcgttta
      781 aagccctcat ttacccatgt aatttcgcct gtattgctaa taataattac tgagttaccc
      841 gcctggctga tggcagttgt gatgctgtcg aactgatccg atagggtttt gagttttttc
      901 agcatacgtc cgcgctgaat ccctaccatt gttcgctgga taagttcagg ggtgtttatt
      961 ggcaagccaa taaagtcgtc ggcaccagcg tggaatagcg attgtatatg ttccggttgt
     1021 aagaaatcgg caatggccag tatggggatg ttagatgtag ccgggtcggc ctttatgatt
     1081 gatagcacat cggcggcagc cttgttggca aagtggatat cgtaaaggat aatgtccggg
     1141 ttgttgttgg tgatagttga aataatacgc tcggaatcaa gaatattact taccgtaaaa
     1201 tcatcgtcgg caaggctgaa tattcgttga atatcttcta aaagttgaat gtttccacaa
     1261 gctgttaaaa ctttgtaagg catttttcaa ttccgatctt aaagcaaaaa aacgaaaaat
     1321 tatgtattaa tggaaacgtt tgcggaaatt atttttcgcc cgcatccaag taaaaagcat
     1381 tttttactca tcatctttct gcatctggtc aataattttt tgaatttcct cctcggtaaa
     1441 gtgaagtttt tgtttgcaga attttagtag ttccgaggcg cgtcggattt tctccgttaa
     1501 ctcatcaata tcaagttcgt tcgattctat ttggttgata attctctcaa cctcctcaat
     1561 agcctcgcta tacttaacag gtttcttggt catacattac attttttgcg ggtaaagtta
     1621 ttgtaaatgt tgttcccttt cccagggtac tacttacgct tatggtgccg ccattagcct
     1681 cggcaaattc cttacaaagc attaatccta gtcctgttcc gctctcggaa ttggttccct
     1741 tggtagtaaa accatcgagc cgaaataggt tactcaaatt ttcaggggct attccaacac
     1801 ccgtatcggt tacttgaatt gtcacttttt ggttatcgga cgaagccttt aatgaaattt
     1861 tgcctccacc tggggtaaac tttatggcat tgctaagcag attccggatt atggttagta
     1921 gagtgtcgta gtcggcaaaa gcggtcactg tttcattgca tattacctcg atttttattt
     1981 ctttttcacc tgcaggcaac gatgccactt cgacagcttt gctaattagt tcgcaaatat
     2041 ttatgtatgc ttgatttaac actaacttgc cagtttgact ccgtgcccag ctaagaaggt
     2101 tctcagtgag tgcaagtaca tttgaggctg actggtgtat gtggttgcta aactctttca
     2161 cctcggaggg cgggagttca tcggctttag tggccaaaat ctccgaaaga cctacaagag
     2221 cggtgaaggg gcttcgtaaa tcatgagcta caatggaaaa cagtttatcc ttggttttaa
     2281 tgttttgttc aagctccgcc tggtattgct ttaatttttg gtttatcctt tcctcctcct
     2341 catatttctc ccgttggagt cgggctaaat tctttttttg gttgaaaagg tagaaactcc
     2401 caaatgcaat aaaaagcaga aaaattactg ctgttaccag gagattgcgc tgtgcttttt
     2461 gcttgtttat gcgttcccta tcgagttcaa ccttggttcg caatgcttct acctcggctt
     2521 caatcagttt tatttcagtc ttggttttat ccagttccag gttgatatgt gcatcaatgt
     2581 ttgcctcgct gcgtttggca tcttcaatgc tatccttaag gtaaacatat ttttggtaaa
     2641 gggttaaggc gttctggaac ttaccctgtg ccttttcaac atcgcttagt gctagggttc
     2701 cgagcaaaac aaggtatggg ttgccaacgc tttttccaat acgtattgat tgggttatat
     2761 ttacttttgc gtccctgtat cttccttgga gtgagtaaat ctcacctatt tttctaaggc
     2821 aaagcgcttc gccatcggca tttaagtttt gcttatattt ttcctttgcg gtgttataat
     2881 gggttagcgc caggtcaaag ttattatcct ggcgttcaat gttgccaagg gtgttatata
     2941 agtccgcaag taactgattt tggttacttc tcagtgcaat ttttattgct tgggttatgg
     3001 tatttctggc tttacttggt ttatttaaat cgaggtaggt ttgggcaatg tttcttagca
     3061 cccgggtaac ctgtaggtca tcacctttct tctcgctcag ttctaatgct ttctggtaag
     3121 cgttaatggc attggtatat tgtttctggt catagtatac attacccata ttttggtata
     3181 tgtatatctt tagatccggc ttagcgtcaa ggttttcaac taagcctttt gcttcactca
     3241 tatacttttg ggcattgcgg aataagccta ggcgccgata aaccagggca atgtttacca
     3301 tggtgcgagc aatatcctca tctttcccat acgactggcg gatttttaga gccaacaggt
     3361 acctgcggag cgcctcaggg taatcctcaa tggttaaata ggtattccct agctcattgt
     3421 ggatattggc ttcagtgagc gggtctccaa tttcttttga tagttccagt gccttgttga
     3481 gcgatgttat agattcgctg ctatttttta gcagcaagta ggctttggcc aaattgttca
     3541 gggtgttaat gctcgactgt ttatagccaa gtgttcccct gatgtttagg ctttgtttgt
     3601 agtattcaat ggcctttgca ggctgagaaa acttaaggta aactccgccc ataatattta
     3661 aaatatcggc cagctcatcg tttagcttgc ttttttctgc tatttgaagg gctttgctaa
     3721 gatgacttgt cgatttgtcg aaattgcctg tttcgcggta ggttagtccg atattcttta
     3781 aaagttgaat aattctaagg gtgtcgttat ccttttcagc tgcaataagc ccttcaaagt
     3841 acgatttaag tgcctcctgg taattaccaa agttccattg agtgctgcca atgctttgat
     3901 ggcattcagc aatgcctaca ccaatttttt ttgcaatggc taatgcttca ccctttttgc
     3961 tcaaggcgtc gttatactgg ccaatttcct tgtaaatatc tccccgtagt agcaagcttt
     4021 gaataagtaa gcggttattt ttccctttca aagctgtaag ccttgccttt tcattaacct
     4081 tgatagcttc gctgtaatta cccgatcgtt gcagtaatgc tgattttttt aaaagagcat
     4141 caacgtataa ggattgggtt cttttggatt gtgtgatggt aattgctgaa tcaacatggc
     4201 ctaaggcatc gccagttaag cccagtccac tcttgtattc gctcatggca aggtggtata
     4261 gcgctctaag tgaatcgctg gggttttgtt tcacctgtaa gaaaagtcga ttgcagctta
     4321 tggctgaaac ttcaggattc tcaatggagt actttttgat ggtggcaatt aagctgtcaa
     4381 tattgacctt tgaaccctgt gaaaaggagc gaaaggccaa cagggctaaa aaaaggggaa
     4441 ctttaatttt ttgcaacatt ttaattacta taagtgatgc tcatagcaaa gtaaggaaaa
     4501 aaaatgatta tgacaaacaa atgggatggg ttatgcaagt ggtatagtaa agtgaaactc
     4561 gctaccactc cctttagagc tgtttaccca aattttacca ttgttctttg ttacaaactc
     4621 gtggcaaagc agtagcccca gtccggtacc cgtttcattg gctgtgccct ttgtgctgta
     4681 ggaaccgtca atcatgaaga ttctttcaag gtcatcctga tctatgccta tacctgaatc
     4741 cttaacaacc acttctgcca ttttaggaaa atctttcgat tcacttgcaa ttatctcgat
     4801 agttcctcca atatcagtaa atttgattgc attactcaat aggtttcgga ttacggttgc
     4861 tatgctattt ttatcagcgt aaacactaat tgctgaactt gcgttgacgg aaacatttat
     4921 cctttttttc tcggcatttg cgttaaggtt taacacttcc tgctttatca aaggctcaag
     4981 tttgaatttc tcgggaatgt agttaatgct accggtttgg gctcgtgtcc actgtaaaag
     5041 gttatcgagc agcttgtaaa gattctgagc cgactcagag attaccgaga tgtattcttt
     5101 cttttcgtta tcggtcaggt ggttaaagtt gcgttcaaga atatcgctaa acccgattaa
     5161 ggcgttaaat ggatttttta aatcgtgggc aataatggag aatagtttat ccttggtggc
     5221 attcagctcg ctaaggtttt tctctgattc tttcagcttt ttgtttattt caaataattc
     5281 agcgtttttc tcagccagtt gcttattctt tttccgaatt atggttaaaa ttaatgatgt
     5341 gccaaagaat atagccagca ataaaagagt tagggaaatt aatacttgct gtacgcttcg
     5401 ttgtttttgg aactgtaact cctttagctc aacatctttc ttcaggagtt ctatttcttt
     5461 ttctttggtt tcggtttcgt accgtgcctg catttcggca atgctggaaa ggcgttgctg
     5521 ggtatagaga gtatcggcgt atgcaagttc aacacacttg taatggtaag catttctata
     5581 atcgcctttt tttgcataga ggtcgctcag tagctggtag gtttcctgtt tgtaggcttg
     5641 atctacaaca ttgggaatgt ttcggagcgc tttattaagc gcttcacttg ccttttcaag
     5701 attattttgc ttgaggtatg ttgatgcaat attgtttagt gtattatggg cggcgtcaac
     5761 tccatttgtt ttcactgaaa tttcataaga tttttggtag ctcatcaagg ctttgtcaaa
     5821 ttgtttaatt ctggcgtata ggaaaccaat gttgttgtaa gcggttgcta tgcccgatga
     5881 atccctttgt tgggtagcaa attccagcga ttttgtataa tactccaaag ccttctgcaa
     5941 atcgcccttt tcgtcgagta ctattcctaa gttgttgtag gcagtgctaa gtccactaat
     6001 gtttttgctt ttttcccact ccttaaaagc aagggtatag tacttctcag ccagttccaa
     6061 atttttcaaa tccctattca gcgatcctat cagattgtac aggttagcct ttaattcgtc
     6121 aatattgttc tcgtttacaa tatttaatgc tttaaaggcg tattctaacg ctttattttt
     6181 atcgtttatt tggttgtaaa ggcttgcaat acggttatat gagattgatt gtaaatcgta
     6241 tcgtttggct ttttcgtaat agtctgctgc cttagtatga ctgctaactg ctagattgaa
     6301 ttgcttaagc tctctgtaaa ccagcccaag attatgatat gaatttccaa tttttaaagg
     6361 gttgccattt agtttcctga actccaacga tttcaggtag taatttaagg ctgtttcgtt
     6421 ttggtacttt gaggagtagg cattccccag ggcgttatag gcatcgccta tagcatcgtc
     6481 gctgccatcg gcttttgcct ctttgagcga tttttcagca aaaatgacgg acgagtcagg
     6541 gtttacatcc cagtagtatt ttgatatatc aataagaa
//
LOCUS       DAOSAL010000104         6329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_7843_length_6329_cov_46.738604, whole genome shotgun sequence.
ACCESSION   DAOSAL010000104 DAOSAL010000000
VERSION     DAOSAL010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 6329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6329
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7843_length_6329_cov_46.738604"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3516)
                     /locus_tag="PK049_10690"
     CDS             complement(<1..3516)
                     /locus_tag="PK049_10690"
                     /inference="COORDINATES: protein motif:HMM:NF019314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOK62068.1"
                     /translation="MENPIVIGILENAAILIASAVLYDFIWLKSDKSRKIRTQILLGL
                     LIAGIGIVLMNSPWILVEGIVFDTRSVLLTISGLFFGGVTTVVAAVIVATSRLMMGGA
                     GMYMGVAVIVSSSLISLLWRKVRPDIIANKNLGEVYLVSIIVHLVMLGCTIFLPEENR
                     MATLLVIWWPVILIYPLATVLIAKLLFSRKENWKQKDKLKESEERYRLIFENNPNPMW
                     IQDLETLNFLEVNRAAITHYGYTREEFLNMTLKDIRHEDDLSKLNGDIQALDGIYQTS
                     FSCIHKKKNGEIIHVQVASHRITYKGRPAKHVMVSDVSELVKSHKLLSQKEQEFREIF
                     NSGFDSIQIVDTESGKIIDCNNITMEMYGFTKEEIINGIIGDLSANIEPFTKEKAQEY
                     IKKTIKGEPQDFEWLARKKNGETFWVNVRLKLVEIGGVNRVMAIIRDISDRKRTEQNF
                     IESEQRFLRVMHFSKDAIGILDENFHFIDCNDSAARLHGFKSRIELLKNSPHPSTLSP
                     QTQPCGSSSYELANKMIEIALEQGFNKFEWLHKKVNGQKFWAEVSLTPIVFNGKRMIY
                     AVWRDISDQKNKQNRIERLLKILDESINEVYIFRCDNLKFIEFNKTAVNNLGYSPEEL
                     LELTALDLKPYLTAEKFNELLKPLNDGTSSKIYFETIHRRKNGTEYPVEVYVEKTKAS
                     DIDVYNAIVLDISQRKKYLAELKQREHELREIFDSTLEAILIYDIETSKIVDCNRQTQ
                     TLYGYSRDELIGMPLGTLSANDEKYNIDEATKWAEKALTVGPQTYTWLGKKKSGETFW
                     VEISLKKSTIGGVDRLMAVVRDITERIAHQHEIERREKEYSEIFNATAEAILIYDPHA
                     NLIVDCNNRAVEMYGYNDKRGFLSIGLNALGANVEPYTNDKFGYYTQKAAKEGPQRYD
                     WLAKRKNGEHFWIEVNLRLTKIGGVERELIVIRDITDRKKFEDDLVKAKEKAEESDRL
                     KSAFLANLSHEIRTPMNAIMGFGDLLKYSQDEQKRKEYIDIIQKSGFRLMDIINETVE
                     IAKLDTGLIKTNPETFNLDQLMQDIYNELKIKISGSKELELIYLPPNTTSETYIYTDR
                     VKLQQILINLITNGIKYTPRGAVAFGYEVKDNRVVFNVKDTGIGIDRKNREMVFKRFY
                     RVENPLTIKVGGIGLGLAITKAYTEL"
     gene            4039..5619
                     /locus_tag="PK049_10695"
     CDS             4039..5619
                     /locus_tag="PK049_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HOK62069.1"
                     /translation="MIFEDEVKRRRTFAIVSHPDAGKTTLTEKLLLFGGAIHIAGAVK
                     SNKIKKTATSDFMEIERQRGISVSTSVMEFEYQNYKINILDTPGHQDFAEDTYRTLTA
                     VDSVIIVIDHAKGVEAQTRKLMEVCRMRKTPVIIFINKLDRPGNDPFDLLDEIEKELQ
                     IKVRPLSWPISQGPSFKGVYNLFEQKLFLFSGENKQTIADDIVEISDLNSPELDSYIK
                     PYTNKFLEEIEIVNEVYPEFDIKTYLSGEVAPVFFGSALNNFGVKELLDCFVQIAPHP
                     GPITTDTRTVTPFEQKFSGFVFKIFANMDPNHRNRIAFLKICSGRFERNVPYFHVRLG
                     KSFRFSSPNAFLADKKSIVEFAYPGDIIGLPDSGNFKIGDTLTEGEVMNFKGIPNFAP
                     ELFRYIENADPLKYKQLAKGIEQLTDEGVAQLFTQKSNGRKIIGTVGALQFDVIQFRL
                     EHEYGAKCRYEPINLYKACWIETNNKAQLDDFRIRKYNNLALDKHGREVFLADSPYSL
                     QMAIEKFPDIKFHFTSEF"
BASE COUNT         1798 a         1378 c         1143 g         2010 t
ORIGIN      
        1 aagctcagtg taagcttttg taatggcaag cccaagtcca ataccaccca ccttaattgt
       61 taatgggttc tctaccctgt aaaatcgctt aaaaaccatc tccctattct tacggtcaat
      121 cccaatgccg gtatccttaa cgttgaacac cacacggtta tcttttactt cataaccgaa
      181 tgccaccgct ccacgagggg tgtattttat tccgtttgtt atcaggttta ttagtatttg
      241 ttgcagcttg accctgtcag tataaatata agtttccgag gttgtgtttg ggggtaaata
      301 gattagttca agttccttac ttccagaaat ttttattttc agctcattgt aaatatcctg
      361 cattagctgg tcaaggttaa aggtttcagg attggtttta attagcccgg tatcgagttt
      421 ggctatctca acagtttcat taataatatc cattaaacgg aagccgcttt tctggataat
      481 atcaatatac tcctttcttt tctgttcatc ctgtgaatat tttaaaagat cgccaaaccc
      541 cattatggcg ttcatgggtg ttctaatttc gtgcgacagg ttggccagaa aggccgactt
      601 taacctatca ctttcctcag ctttttcttt tgcctttact aggtcatctt caaatttttt
      661 tcggtcagta atatcgcgga ttactataag ttcccgttca acacctccaa tttttgtaag
      721 acgaaggttt acttcaatcc agaaatgctc accgttttta cgttttgcaa gccaatcgta
      781 ccgttggggg ccctccttgg ctgctttttg ggtataatat ccaaatttgt cgtttgtgta
      841 tggctcaaca ttggctccaa gggcattcaa cccaatgctc aaaaaccctc ttttgtcatt
      901 atacccatac atttcaactg ccctattgtt acaatcaact ataaggttag catgtgggtc
      961 ataaatcaga attgcctcgg cagtagcgtt gaatatctcg ctgtactcct tctcgcgtct
     1021 ttcaatttca tgctggtggg caatacgttc ggtaatatcg cgcactacag ccataagtct
     1081 atcaacccct ccaatggttg actttttgag cgatatctct acccagaatg tttcgccgct
     1141 tttcttttta cccagccatg tgtaggtttg tggtccaact gtcaacgctt tctcagccca
     1201 tttagtagct tcatcaatat tgtacttttc atcgtttgca ctgagggtac ctaatggcat
     1261 accaattagc tcatccctgc tgtatccgta aagagtttgt gtttgcctat tacagtcaac
     1321 aattttactg gtttcaatgt cgtatatcaa aatggcttca agggtagagt caaaaatttc
     1381 gcggagttca tgttcgcgtt gtttcaattc agccaaatat ttttttcttt ggctaatatc
     1441 taagacaatt gcgttgtaaa catcgatatc tgaagccttt gttttctcaa catagacctc
     1501 aacgggatat tcagtaccat tcttacggcg atgtattgtc tcaaaatata tcttagatga
     1561 agttccgtcg ttaagaggtt ttaacaattc gttaaatttt tcagctgtta ggtatggttt
     1621 taaatcaagt gcggtaagct caagtaattc ctctggtgaa taacctaagt tattcacagc
     1681 agttttgtta aattcaataa attttagatt gtcgcatcgg aaaatatata cttcgtttat
     1741 actctcatcc aatatcttta gtagtctctc aattcgattt tgcttgtttt tttgatcaga
     1801 tatatcgcgc catacagcat atatcatccg ttttccatta aatacaattg gtgttagcga
     1861 cacctctgcc cagaattttt gtccatttac ttttttatgg agccattcaa atttgttaaa
     1921 accctgttca agtgcaattt caatcatttt gttggctagc tcatacgatg aactcccaca
     1981 aggttgagtt tgtggactta gagtcgaagg gtgaggtgag tttttaagca gttcaatacg
     2041 cgatttaaaa ccatggagtc gtgccgcaga gtcattacaa tcaataaagt gaaaattttc
     2101 atccagtatt ccaattgcat cctttgagaa atgcattaca cgtaaaaacc gttgttcact
     2161 ttcaataaag ttttgctctg tcctttttct atccgaaata tctcttataa tggccattac
     2221 cctattaaca cctccaattt caactagctt gagtcgcacg ttaacccaaa atgtttcccc
     2281 atttttttta cgtgctagcc attcaaaatc ctggggttcg cctttaatgg ttttttttat
     2341 gtactcttga gctttttctt tggtaaaggg ttctatgttt gcacttaaat caccaataat
     2401 cccattaata atttcttcct ttgtgaaacc atacatctcc attgtaatat tgttacaatc
     2461 aattattttt ccactttcgg tgtcaactat ttgaatggag tcaaatcctg agttaaatat
     2521 ctccctgaat tcttgttctt tctggcttag taatttatgc gacttaacca attcacttac
     2581 atcgctaacc atgacatgtt tcgctggtct tcccttgtaa gtaattctat gcgatgctac
     2641 ttgaacatgt ataatttcac cgttcttttt cttatgtatg cagctaaagg atgtttgata
     2701 aataccgtca agggcttgta tgtcaccatt caattttgaa aggtcatctt catgtctgat
     2761 gtcttttaga gtcatgttta aaaactcttc cctggtataa ccgtaatgag taatggcagc
     2821 cctattaact tccaaaaaat ttagtgtttc aagatcttga atccacatgg gattgggatt
     2881 gttttcaaaa atcaaacggt atcgctcctc gctttcctta agtttatctt tctgtttcca
     2941 gttttccttc ctactaaaaa gtagctttgc tattaggact gtagctaaag ggtaaattag
     3001 tattactggc caccatatta caagcagggt ggccattctg ttttcttcgg gtaagaaaat
     3061 tgtgcatcca agcatcacca ggtgaactat aatgctaacc aagtaaacct caccaaggtt
     3121 tttattcgca attatgtctg gacgaacttt ccgccaaagc aggctgataa gcgatgaact
     3181 tactattact gccactccca tatacatccc agcaccaccc atcatcaatc gggatgtagc
     3241 aacaattacc gctgcaacca ctgtggttac cccgccaaaa aacagtcccg aaatggtgag
     3301 caataccgat cgggtatcga atactatccc ttcaactaat atccatgggc tattcataag
     3361 aactatacct attcccgcaa tgagtaggcc aagcaaaatc tgtgtgcgaa tctttctact
     3421 tttatcggac tttaaccaaa taaaatcgta tagaactgca gatgcaatta gtattgctgc
     3481 attttcaagt attccaataa ctatgggatt ttccatggat gtaaaaaatt tggtatcacc
     3541 ataaatttag gcaatatttt atcttaaatc aataatcaat aatcaataac caatattctt
     3601 tagttattga tatatgtcat attgagtcct gatataccaa gaaatactcc attgaaacat
     3661 ctctctttta tgctgaaccc tgccgaagca tcactttacc tttataaccc attaatggat
     3721 ccctggctgc gatctgaatg acaaaaggat ttgacggttg attgactcaa aattgaacgc
     3781 aaattgcact tattaagttg atttacacag gttaaactaa ccaaacccaa aacactaaac
     3841 accaaacaca atcacccctg tacactgaac actgcacact ataaataaaa tccctcgcaa
     3901 cgctcagaat gattaaaggg atgtaaacta tttaaggcta atcatcataa atcatcaacc
     3961 cttactttct ttttcttcgc accttaattc atttaaaggc taataattgc taattttgca
     4021 agccataaat gaagatatat gatttttgag gacgaagtta agcgccgccg aacatttgcc
     4081 attgttagcc atcccgatgc cggtaagaca accctaaccg aaaagttgct gctctttggc
     4141 ggagcaattc acattgcagg tgccgttaaa agcaataaga ttaaaaagac tgccacgtcc
     4201 gatttcatgg agatagagcg ccagcgtggt atttcggttt ccacatcggt aatggagttt
     4261 gagtatcaga actataaaat taacattctc gatacacctg gtcaccagga ttttgccgag
     4321 gatacatatc gtacccttac tgctgtcgat agcgttataa ttgttattga ccatgcaaaa
     4381 ggcgttgagg cgcaaacccg caagctcatg gaggtttgcc gcatgcgtaa aactcccgta
     4441 atcatcttta tcaacaagct cgataggcct ggcaacgacc cattcgacct gctcgatgag
     4501 attgagaagg aacttcagat taaggtacgc ccgcttagct ggccaattag ccaggggcca
     4561 tcgttcaagg gggtttacaa cctttttgag cagaaactct tccttttctc cggtgagaat
     4621 aagcaaacca ttgccgacga tatagttgag atatctgatt tgaactcgcc tgaactggat
     4681 tcttacatta agccctacac caataagttt cttgaggaga ttgagattgt taacgaggtt
     4741 tacccggagt tcgatatcaa aacctacctt tccggcgagg tggctcccgt tttctttggt
     4801 agtgccctaa acaactttgg ggttaaggaa ctcctggatt gttttgtaca aattgctccc
     4861 catcccggcc ctatcactac cgatacccgt acggttacac catttgagca aaaatttagc
     4921 ggatttgtgt ttaaaatttt tgccaacatg gatcccaatc accgtaacag gatagcattc
     4981 ctaaagatat gctctggtcg gtttgagcgt aatgttccat acttccacgt aagattagga
     5041 aaatcgttcc gtttttccag ccccaacgca ttcttggccg ataaaaagtc gatagtggag
     5101 tttgcatacc ccggcgacat aattggcttg cccgattcag gcaatttcaa gattggcgat
     5161 acccttactg agggcgaggt tatgaacttt aaaggtatcc ctaactttgc ccctgagctt
     5221 ttccgctaca ttgagaatgc cgacccctta aagtataagc aactggcaaa aggaattgag
     5281 cagctaaccg atgagggtgt agcccagctc ttcacccaaa aatccaatgg taggaagatt
     5341 ataggtacag tgggtgcttt acagtttgat gttattcagt tccggttgga gcatgagtat
     5401 ggtgctaaat gccgctacga acccataaac ctttacaagg cttgctggat tgaaaccaat
     5461 aacaaggccc aactcgacga tttccgtatt cgaaagtata ataaccttgc cctcgataag
     5521 catgggcgtg aggtattcct ggccgattca ccttactccc tacaaatggc tattgaaaag
     5581 ttccccgata ttaagttcca cttcacttca gagttttaat ataggttgtt agttgtttgt
     5641 tgatggttga tagttattac aaaatgtttg acacagtaaa gtactgtgaa accctgtatt
     5701 aatttgtgat acctgataaa ctttattgag gaaatttttt caggtttacc gtgaacagtc
     5761 ctgtaggagc taaacattta taactccatt gtcattccga gcaagtcgag gaatctcatt
     5821 aaatacggga tgatagcaca tagagatgct tcggcaagct cagcatgaca cgataggacg
     5881 gggttacacc aaactatcaa caatcaaaca acaaccatca acttaaatcg tttcacaggg
     5941 taaatccacc caatctgcgt catctgcatt ccattgcatt agcaagtatc acagagttac
     6001 acagtgtaac acggtgtttc acagaattgc aatcacgaac aagcatcaac tttatatttg
     6061 tcatcctgag cgcagcgaag gatctctaat cgacacattg agatgtttcg cttgcgctca
     6121 acatgacaat agtttcacga gcaacgaaca acgctcccag ccccataggg gcgtaatatt
     6181 tgtaacacaa tagttactcc ctcatgcata gcgatgcacg ccatggcatt ccaaagagca
     6241 agggtgaaac attgcatcgc aaaagaaaaa acagggcaaa gagcggttta catgttcttt
     6301 cttgtcttga tacaagaaag aaccaaaga
//
LOCUS       DAOSAL010000105         6122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_8177_length_6122_cov_48.680567, whole genome shotgun sequence.
ACCESSION   DAOSAL010000105 DAOSAL010000000
VERSION     DAOSAL010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 6122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6122
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8177_length_6122_cov_48.680567"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="PK049_10700"
     CDS             complement(<1..545)
                     /locus_tag="PK049_10700"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF013071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HOK62070.1"
                     /translation="MFGRDGFNKNRVRELKVGEVKGKGPIIYWMSRDQRVADNWALTY
                     AKLKANEHKVPLRVVFTLTAAFPAANLRHYDFMLKGLAEVEKSLAHEGIPFNLLRGEP
                     AEQLWEFAKRVEASLIVSDFDPLWVKRDWKSQLNQRIAIPHHEVDAHNVVPCWYASPK
                     LEFGAYTLRPKIKKHLSTFLT"
     gene            complement(1065..2096)
                     /locus_tag="PK049_10705"
     CDS             complement(1065..2096)
                     /locus_tag="PK049_10705"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOK62071.1"
                     /translation="MKKILLFAIFASGVTITAAQVKLTQETHGFFADLKNPMVLTSYV
                     EPGQSGSNVVWDFSKLQVQNSFTGNIEGFYTSKCCSKFTKGNVVLEEFGNFFVFESTP
                     TSLEQVGYMSANGVTRFEYSKPFIKMRYPFSYGDFYSGNYEGNYIVNDNVIGTISGTY
                     QVEGDGFGKLILPNGQTLNNVLRVKEVRTTKQVFSSGSALITDITYRWYVAQHRFPVL
                     VLIRSEVKGENSEPVVSTRAAYNSNVMNTTTSIPTNQISALSLSAYPNPYTDRVKIEF
                     TMAQRDQVNLSIYDMSGKLVKEIENNILDSGLKHYEFSAKSLGLTSGAYILKLKVGQI
                     EETRKLLEL"
     gene            2209..2895
                     /locus_tag="PK049_10710"
     CDS             2209..2895
                     /locus_tag="PK049_10710"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF007752.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HOK62072.1"
                     /translation="MKIVFILVEPAVPENVGAAARAFKTMGFNDLRLVNPCDYLSVDA
                     RKLAHASNDILENASVYNTLEEALHDIDFAFATSARDRWIKLDIIDSHEMPKFIEGKG
                     DTISNVAVVFGREESGLTNHEINLCQRVTTIPLKTKYPSLNLAQSVMVYAYILSGINL
                     GENQAAKADTTQSFQALSKKVDAVMKAIDLGPDKLIYGRLMERLSELGEQDIKLLHSI
                     TTELIQKLNR"
     gene            complement(3008..3388)
                     /locus_tag="PK049_10715"
     CDS             complement(3008..3388)
                     /locus_tag="PK049_10715"
                     /inference="COORDINATES: protein motif:HMM:NF022317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HOK62073.1"
                     /translation="MQIILQILDYFFVGLHTFIVLFNLFGWIWARFRRWNLALLILTG
                     LSWFGLGIFYGWGYCPLTDWHFRVLQGLGRTPQETSYISYLLNRLLQLKVDDTLVDSA
                     TLICFMVALVTSVILNVRGLRLKD"
     gene            complement(3392..4594)
                     /locus_tag="PK049_10720"
     CDS             complement(3392..4594)
                     /locus_tag="PK049_10720"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOK62074.1"
                     /translation="MEQQKKSPIIAILTGGGDVPGLNPAIRAITIRALREGFSVIGIR
                     RGWAGLIEIIRDKHVDNSHCFEVLTEERVNKAFRTGGTFLHTSRTNPAKVAKDNVPEH
                     LKAKYNKDINDLTPEVIKNLSFLGIDYLIPIGGDDTLSYGVRLYQEGFKVIAIPKTMD
                     NDVTGTDYCIGFSTCVTRTIALTDMLRTSAGSHERILVMEVFGRYAGFTAMLPTMAGV
                     ANRCVIPEYKFNIERLTELLVEDRNRNPSKYSTVLVSEGVTFEGLEQMVFQSSERDAF
                     GHAKLGGIGEIVSRKIKELTPKFNNGKTINIISQNLGYLVRGGEPDAIDSIVPMAFGN
                     LALDLILKGVHGRLVVLKNGRYDNMPIDVVTSKKKIVNVERYYNTERLRPFYKSFEMQ
                     PLFIMTSE"
     gene            complement(4778..6064)
                     /locus_tag="PK049_10725"
     CDS             complement(4778..6064)
                     /locus_tag="PK049_10725"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOK62075.1"
                     /translation="MRRLCVMSNTRFLTDAQLPFCKGCGHALVAQNTEKALQKLNINP
                     LDVVLVTDIGCHGIVDKSFLTHTVHGLHGRSSALAAGITAGLNNPDKKVIVFTGDGGA
                     TIGMQHLIGGAHLGFDMTVVVHNNMLYGMTGGQPSEFTPCGFKTPTLPEGSTKPGYDI
                     CELMVAAGASYVERVIGIGDYSDSLVKAFTAPGFSLVEVMEICPSYGVKSNPGMKLSK
                     LVEDAGWNVKVYADGKGNSFKTPINSEPKSLISDKFEVKPKYSSSIERPVSIMLSGSA
                     GEGVQSAAEFLAKAAIVSGLNSTKKGSYPVTVGVGFSASEVIVSPKQILYTGSPVPDV
                     LIITSTDGLGYARAAAGRMKGGVIYIDETLDAPETGARIVKVPFRERVGAKNSAMYAV
                     FYAVHYEKFYPIEALKDVFLSNKIAEKVNIESLLQF"
BASE COUNT         1824 a         1399 c         1233 g         1666 t
ORIGIN      
        1 tcggtcagga aggttgatag gtgtttcttt atcttgggtc gaagggtgta tgccccaaac
       61 tccaatttag gcgaagcata ccagcatgga acaacattat gggcatcaac ctcgtgatgc
      121 ggaatggcaa tgcgttgatt gagctgcgac ttccagtcgc gtttgaccca taggggatcg
      181 aaatcgctaa caataagcga ggcctcaacc ctctttgcaa attcccagag ctgttcagca
      241 ggttcacccc taaggaggtt gaaagggatt ccctcatggg caaggctttt ttcaacctcc
      301 gcaaggcctt tgagcatgaa atcgtaatga cggagattgg cagcagggaa tgccgcggta
      361 agggtaaata caacccggag gggaacctta tgctcatttg cctttagctt ggcgtaagta
      421 agagcccagt tatcggcaac gcgttggtca cgcgacatcc agtagattat tgggcccttc
      481 ccttttacct cgcccacctt aagctcccta accctatttt tgttaaaacc atccctgccg
      541 aacatctatt tatagcttta aggtaaaaat agccaaaaaa cattgagttc ttgaaatctg
      601 tttaataaat tttagggaaa taggtcaata tcatgcccat gaaccatgaa ttagcaaatt
      661 caaccatgct gatttttgat gaatgagtcg gaatgaatcg gaataggcag aatatgatgg
      721 agttagagga agttagagga agttagagga attgtgaaaa gtgaaaagtg aaaagtgaac
      781 agctaaaccg aagtaccatg aattatcaaa taaaaaagga cttaaaaaaa tggggatgcg
      841 caaacattat tagaggatat ttccaattga atttttcaat tgacgaatcg tctaacaaaa
      901 tctaatcgtt ccgagatatc gaaatgtaag attttgataa gtcgggatta cctcgataag
      961 tcaatagaaa caagttattt gaatgtttta atgtatttct attgactagt cttggttaat
     1021 ataaaagccg ggattacccg gccttcaata aagatattta acatctataa ctccaacagc
     1081 ttgcgtgttt cctcaatttg accaaccttt agcttgagta tgtaagcacc actggttaag
     1141 cccagggact ttgctgagaa ctcatagtgt tttaggccag aatcgaggat gttgttctca
     1201 atttccttta ccagcttgcc gctcatatcg tagatgctaa ggttaacctg atcccgttgg
     1261 gccatggtaa actcaatttt gaccctatcg gtgtatggat tagggtaagc gcttaagctg
     1321 agagctgata tttgattggt tggaattgat gtggtagtat tcatcacatt gctattatac
     1381 gcagcacgtg tacttacaac tggttctgag ttctcgcctt tgacctcgct acggattagg
     1441 actagtactg gaaacctatg ctgagctaca taccaacggt aggttatatc ggtgattaaa
     1501 gccgaaccgc tactgaatac ctgcttggtg gttctaacct ccttaacacg aagcacatta
     1561 ttaagtgttt gaccgtttgg aagtatcaac tttccaaagc catcgccctc aacctggtaa
     1621 gtgccggaga ttgtaccaat tacattatcg tttacaatat aattaccctc gtaattgcca
     1681 ctgtaaaaat cgccatagct aaatgggtaa cgcattttaa taaagggttt gctatactca
     1741 aagcgggtta ccccatttgc cgacatgtaa ccaacctgct caagacttgt tggggttgat
     1801 tcaaaaacaa agaagtttcc gaactcctca agtaccacat tacccttggt aaacttgctg
     1861 caacactttg aagtgtaaaa accttcaata ttaccggtaa aagaattctg aacctgcaac
     1921 ttgctgaagt cccataccac gttgctcccc gactgtccgg gctcaacata gctagttaga
     1981 accattgggt tctttaaatc ggcaaagaaa ccatgggttt cctgcgttag ctttacctgg
     2041 gcagcagtaa tggttacgcc cgatgcaaaa atggcaaata gtaaaatctt tttcatacga
     2101 ttaccctttt gtgtaaaaac aaatttaatc atacagccaa ctcaaatcaa tagattaagg
     2161 caaatagttc taatttcatt gttaattttg cgataattcc taaaagagat gaaaattgta
     2221 tttattcttg tggaacctgc tgtacccgag aatgttggcg cagccgcccg agcctttaaa
     2281 acaatgggtt tcaacgactt acgactggta aacccctgcg attacctttc ggttgatgcc
     2341 cgcaagctgg cacacgcctc taacgatatc cttgagaatg ccagtgttta taatacacta
     2401 gaggaagcac tgcacgatat cgactttgcc tttgccacgt cggcccgcga taggtggata
     2461 aagttggata ttatcgacag ccatgaaatg ccgaaattta ttgagggaaa aggcgacaca
     2521 atcagcaatg tggccgttgt atttggccga gaggagtcgg ggcttaccaa ccacgagata
     2581 aatctttgcc aaagggttac tactattccg cttaaaacta aatacccatc gctaaacctg
     2641 gcacaatcgg tgatggttta tgcctatatt ctatcgggaa tcaacctagg cgagaaccaa
     2701 gcagccaaag ccgacaccac ccagtcgttt caggcacttt caaaaaaagt tgatgcagtg
     2761 atgaaggcta ttgaccttgg acccgataag ctaatatacg gtaggctcat ggagcgcctc
     2821 agcgaactcg gtgagcagga tattaaactg ctgcactcca taacaaccga gctaattcag
     2881 aaactgaaca ggtaaatgga aaaataggat ggaaagcaat gaatcagaag tcagtatctc
     2941 atatcaatac aatatgaaac ccattcatcc ttcaactttt ttatccttta tcctttatcc
     3001 tttaacctta atcctttaac cttaatcccc taacgttcag aataactgaa gtaactaaag
     3061 ccaccataaa acaaattagg gtagcgctat caaccagtgt atcgtcaact tttagctgta
     3121 aaagcctatt taacaaatag gatatatacg atgtttcttg aggtgttctg ccaagtcctt
     3181 ggagtaccct gaagtgccaa tcagttaaag gacaatagcc ccaaccgtag aatatgccaa
     3241 gcccaaacca gctgagacct gtaagaataa gcaaggccag attccacctc cggaacctgg
     3301 cccatatcca accaaaaaga ttaaatagta ctatgaatgt atgcaaccca acaaaaaagt
     3361 agtcaagtat ttgaaggatt atttgcattt tttactcgct ggtcatgatg aacagtggtt
     3421 gcatctcaaa gctcttatag aacggacgta aacgttcagt attatagtaa cgctccacgt
     3481 tcacaatctt cttcttgctg gtaaccacat caataggcat attatcgtaa cgaccattct
     3541 taagaactac aagccttccg tgaacaccct tcaggataag atcgagtgca aggttgccaa
     3601 atgccatggg gacaatggaa tcaatagcat cgggctcccc gccacgaacc aaatagccaa
     3661 ggttctgact tataatgttt atagttttac cgttgttgaa cttgggcgtt agctccttaa
     3721 tctttcggga aacaatctca ccaataccgc cgagtttagc atgtccaaat gcatcgcgtt
     3781 cactactctg gaaaaccatt tgctcaagcc cttcaaaggt aaccccctcc gaaactaaaa
     3841 ctgttgagta cttacttggg tttcgatttc tatcctcaac taacagctcg gtaagccgtt
     3901 caatgttgaa tttgtactca ggtattacgc agcggttagc cacgccagcc atagtaggaa
     3961 gcatggcggt aaatccggca tatcggccaa aaacctccat caccagtatt cgttcatgcg
     4021 agcctgccga ggttcgtaac atatcggtta atgcaatagt gcgtgtaacg caagtgctaa
     4081 accctatgca gtaatcggtt ccggtaacat cgttatccat ggttttaggg atagctatca
     4141 ccttaaaccc ttcctggtaa agcctaacgc catagctgag ggtatcgtca cccccaatgg
     4201 gtattaggta gtcgatgccc aaaaaagata gatttttaat aacctcgggt gttaaatcgt
     4261 ttatatcctt gttatatttt gcttttaggt gttcaggtac attatccttt gcaaccttag
     4321 cagggtttgt gcgcgaggtg tgtaaaaatg ttccaccggt acgaaatgct ttattaacac
     4381 gctcctcagt taaaacctca aagcaatggc tattatccac atgcttatcg cggataatct
     4441 caatcaaacc agcccaacca cgccgtattc caatcacgct aaaaccttcg cgtaacgccc
     4501 tgatggttat agctcgtatg gctgggttaa gtccgggcac atcgcccccg ccggtaagta
     4561 ttgcaataat tggagatttc ttttgctgtt ccatagcttt aaattttaat ttaaaaacga
     4621 ttaaatataa gtttattctg agctaaagtc aatagaaaaa aagagctacc catattgggc
     4681 agcccctaaa aatgcttcaa ataaactaac ttaaacccaa gttcgtcgat agaagcaaat
     4741 agtttaacta tttattgtgt tcctatcgat atctaggtca aaactgcaac aaactttcga
     4801 tatttacttt ttcggctatt ttattggaaa ggaaaacatc tttgagagcc tcaattgggt
     4861 agaatttttc ataatgaacg gcgtagaaaa cagcatacat ggcgctattc tttgccccta
     4921 ccctctcgcg gaaaggaact ttaacaatgc gagcaccggt ttcaggcgca tccaatgttt
     4981 catcaatata gataactcct cctttcattc tgccagccgc tgcgcgggca tagcccaaac
     5041 cgtcggtcga tgtgattata agtacatcgg gaaccggcga gccagtgtaa aggatctgct
     5101 taggagaaac aattacctcc gaagccgaga atccaacacc aaccgtaacc gggtaacttc
     5161 cttttttggt actattaaga cccgatacaa tggcagcctt tgccaggaat tcggcagccg
     5221 actgaacacc ctcgccagca gaacccgaca acattatgct aactggcctc tctatactgc
     5281 tggagtactt aggtttcacc tcaaatttgt ccgaaatcaa actcttaggt tcactgttaa
     5341 taggtgtttt gaacgaatta cccttgccat cggcataaac tttaacgttc caaccggcat
     5401 cctcaacgag cttgctaagc ttcattcccg gattcgattt tacgccatag cttgggcaaa
     5461 tctccatgac ctcaaccaaa gagaacccag gggcagtaaa tgctttaacc aatgaatcgg
     5521 aatagtcgcc aataccaatc acgcgctcaa catacgatgc ccctgcagca accattagct
     5581 cacagatatc gtaaccgggc ttggtgcttc cctctggtaa agtgggcgtt ttaaaaccgc
     5641 aaggggtaaa ctctgaaggt tgccccccag tcattccata aagcatgttg ttgtgtacca
     5701 caacagtcat atcaaaaccc agatgagctc caccaatcaa atgctgcatg ccgatagtag
     5761 ctccgccatc gccggtaaaa actatcacct ttttatcggg attattcaat cctgcagtaa
     5821 ttcccgcagc caaagccgaa gaacggccgt gtagcccatg aacggtatgc gtcaggaaac
     5881 tcttatcaac aattccatga catccaatgt cggtaaccaa caccacgtcg aggggattga
     5941 tattaagctt ttgtagagcc ttttcggtat tttgagctac cagggcatgc ccacaacctt
     6001 tacagaacgg tagttgggca tcagttaaaa aacgggtgtt actcataacg cataacctcc
     6061 tcaacaattt cggttggact aatcatttta gcaattccgt ttacgccgct aacgccctta
     6121 cg
//
LOCUS       DAOSAL010000106         6075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_8250_length_6075_cov_52.620598, whole genome shotgun sequence.
ACCESSION   DAOSAL010000106 DAOSAL010000000
VERSION     DAOSAL010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 6075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6075
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8250_length_6075_cov_52.620598"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1307)
                     /locus_tag="PK049_10730"
     CDS             complement(<1..1307)
                     /locus_tag="PK049_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HOK62076.1"
                     /translation="MSRKSYVDFLSNIELFRGLSEQELNTLVKGIVEREFPTGSMLFI
                     ENNKREGILVIYEGQVELFKSNPYGVELKLTQFSKGDFLGEGVLASESTHSTSARAIA
                     PTKALLLRVEDLNANPAIALKVLTNITRVMQRRMRYANNRMVNPASQYESGATRLEHD
                     LLGEREVPAEYYYGVQTLRALENFNISGVTLNFYPDLIDALAMVKQAAAEANHELGLL
                     EPTIKDAIIKACTEITNGKFHNQFVVDMIQGGAGTSTNMNANEVIANRALEHLGYSKG
                     EYQYCHPLNHVNMSQSTNDAYPTAVKIALINSNKKLVRVLESLIESFRAKAKEFAHVI
                     KMGRTQLQDAVPMTLGQEFEAYAVTLSEEVERLNQNVRLFLEVNMGATAIGTGINSDP
                     DYPEKVIKHLRKISGLDLVLASNLVEATQDTGAFVMYSSAVKRL"
     gene            complement(1476..2327)
                     /locus_tag="PK049_10735"
     CDS             complement(1476..2327)
                     /locus_tag="PK049_10735"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HOK62077.1"
                     /translation="MYSQVWEKCSASVCCLNFYNEHGVLIDTLSGFKINKSLVTCEQA
                     FYVKGAQKVEIRFVEADANTPKASMRLDYKEFIDDLRIGFTHNQADYAVFNIDFAEFQ
                     DIPSLSLCERWNYPIGMPVAIIGFNGVGQNLSIKTGIVSSAFSNKQGVRYLQLDGLTC
                     YGNSGAPVIDPQTMQVIAIVSRRNNPATKSYNDLMGYISTNLDELRKIEGKFKLGELD
                     PIQVLIANQNQIKLLATNIYKHTATGSSQAVMLDHIITYFNEQAIIENHLLKSTEVEF
                     DVTVNKE"
     gene            2603..5083
                     /locus_tag="PK049_10740"
     CDS             2603..5083
                     /locus_tag="PK049_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK62078.1"
                     /translation="MDLHKQLIEAVEKRFGKKISYQRDCKTLSEKVFEHTHEYISPAT
                     LRRLFGFLKTNSKPTRVTLDILARYCGYSHWQDFVENQIKASVTSKTNIPELWSLITT
                     NTLEVNHHSIRSIQIKSGIPFEKTVRRTYLESLLNHFVSSNYTALAIVAPGGYGKSTF
                     IAHWYLNNPKVKKQQIIPIIISASKLENYAFSELNIDEWLWGQIGINPFSIPNLKDEL
                     KKLPGSVVFIIDALDEVTLTGTKLERTLKGIHELAKEFSESANTKLIITSRYATWKSL
                     QGYLINPEVWLNIDQSNFNSDNANIPPLSYDEMQTVLDNTVNEGKKERLLIFELPPLL
                     RQTISYPFYLQLFINSYGKATYQTLSDPLNILNEFIKQKVFDSIYPEEKADILNAIAK
                     EWLENSGAITKQQLKKLYPINLKTAGNYFDAYHDLLSFGIISEEKVYSDSLGYSTLVS
                     ISNNNILTSLIAQKLIKEYGFNEKLINATKNLFSLNEIYPKLIGYIFSISFNERKVAF
                     LKDLFQLFLPDYITELTNTLTLCLKRDESLFKDLLPHLLKNRELERELLLISPDLNGT
                     SSTYRKILEQVQPSSPYYKDSKIAMDYCNLIGLEISQINNSNEWINNFILTEQSSARI
                     AGMWFCLNIFFSSSSKSELMVKKALEFYKSINSANAKLEFRESVLPLVFIDKRFFPLQ
                     DIFIEPLNHSAHQKALDSIIYEFTDITKNRTPFTPEKHHQLMQNYSQLNPSRNHFAIV
                     LGELARSISYFYESNLNDAHTCIRNSIELCGISGYRLQEMVLMNILGQTLIRLGETNY
                     GKSFVSYAETLSEKSGVKVYSEHFVCGS"
     gene            complement(5046..5903)
                     /locus_tag="PK049_10745"
     CDS             complement(5046..5903)
                     /locus_tag="PK049_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK62079.1"
                     /translation="MIIIADSGATKTDWRIIEHGKPIASFETIGLSPYFNRQEDFLSA
                     LNQNFPKQINPEQVKEIFFYGSGCGVHERGQDVARYLGVFFSNAKIHAVTDALGAARA
                     LFKRQTGIVVILGTGSNIAYYDGERLNNRTPSLGFVLGDEGSGAYMGRLLLRAYHYKT
                     LDVGLAQKLEQQYNVELSHVLNMVYSAPRPSAYLASFVPFILENIHNQHIYSIVKESF
                     VALYRYHFAVFENLSDLSIGVTGSVGCLFSQVLDSVGEEYGFKISQYLRYPIVELINY
                     HTQNAQNTP"
BASE COUNT         1856 a         1331 c         1115 g         1773 t
ORIGIN      
        1 agcctcttta ctgccgatga gtacattaca aatgcaccgg tatcctgggt tgcctcaacc
       61 aagttggagg caagtaccag atctaagccg gatattttac gtaaatgctt tattactttc
      121 tccgggtaat cggggtccga gtttataccc gttccaattg cggtagcccc catgttaacc
      181 tctaggaata gccgtacatt ctgattcaag cgctccacct cttcggaaag ggtaaccgca
      241 taagcctcaa actcctgacc aagagtcatg ggaacagcat cctgaagctg tgttcttccc
      301 atttttatta catgggcaaa ctcttttgcc tttgctctaa acgattcaat cagggattca
      361 agaactctta ccagtttttt gtttgagttg ataagcgcaa ttttaacagc agtagggtaa
      421 gcatcgttgg ttgactgcga catattcaca tggttcagtg gatggcagta ctgatactcc
      481 ccctttgaat accccaaatg ttccagtgcg cggttagcaa taacctcgtt ggcattcata
      541 tttgtggagg tacccgcccc accctgaatc atatcaacca caaactgatt gtggaacttc
      601 ccattggtta tttcagtgca agctttaatt atggcgtctt taatggtggg ttcaagtaaa
      661 cctagttcat ggtttgcctc ggcagcagcc tgtttcacca ttgcaagagc atcaattaaa
      721 tcggggtaaa agttcagggt taccccggag atgttaaaat tctccaatgc ccttagggtt
      781 tgcactccat agtagtactc ggcaggcact tccctctctc caagcaagtc gtgctcaagg
      841 cgggtcgcac ctgattcgta ctggctagca gggttcacca tacggttgtt ggcatacctc
      901 attcgccgct gcataacccg ggtaatattg gtaagtacct tgagtgcaat tgcaggattt
      961 gcattcaaat cctcaaccct caggagtaaa gcctttgttg gagctatagc ccttgccgat
     1021 gtagaatgag tagattctga ggctagaacc ccttcgccaa ggaaatcgcc ctttgagaat
     1081 tgggttagct taagctcaac tccataagga ttgctcttaa aaagttcaac ctgcccctca
     1141 tatatcacca gaatgccctc gcgtttatta ttctcaatga acaacatgct tccggtaggg
     1201 aattcccttt caacaatccc tttgacaagt gtattcagtt cctgctcact caatcccctg
     1261 aaaagttcta tattactcag aaaatcaaca taactctttc gactcatttc cttatcattt
     1321 aagtttcacc ataaaaaact aacacaaaac taagtatgct tgattcttat tgttcatgca
     1381 aattacaaac attttacttt ttataattgt ttaacataaa aaagaaaggg cactgcatga
     1441 tagcagtacc ctaccaaccc atgaaaacaa aaacactact ctttattaac tgttacatca
     1501 aattccactt cggtactttt taataaatga ttttcaataa ttgcttgttc gttaaagtaa
     1561 gtgataatat gatcgagcat aactgcctgc gatgaacctg ttgcagtgtg cttgtagata
     1621 tttgtggcca aaagcttaat ctggttctgg ttggcaataa gtacctgaat ggggtcgagt
     1681 tcaccaagtt taaatttacc ttcaatcttg cgcagctcat caaggtttgt gctgatgtag
     1741 cccattaaat cgttgtacga tttggttgcc gggttatttc tacgcgaaac tattgcaatt
     1801 acctgcatgg tttgagggtc aattacagga gctccgctat tcccatagca agttaggcca
     1861 tcgagctgaa gatacctaac tccctgctta ttggagaaag cggacgaaac tattcctgtt
     1921 tttatggaaa ggttttgccc tactccatta aaaccaataa ttgccacagg catgccaatt
     1981 gggtaattcc atcgttcgca cagcgatagg cttggaatat cctgaaattc agcaaaatca
     2041 atattaaata cagcgtaatc ggcttggtta tgggtaaacc caatcctaag gtcatcaatg
     2101 aactctttgt aatccaaacg catggatgcc tttggggtat ttgcgtcagc ctcaacaaaa
     2161 cggatttcta ccttttgggc gccttttacg taaaaggctt gctcacaggt tacaagcgat
     2221 ttattgatct taaatccact caacgtgtca atgagcacac cgtgctcatt gtaaaagttc
     2281 agacaacaaa ccgatgctga acatttttcc caaacctgac tgtacatggt tttttaaatt
     2341 tataatgtta aagtttttac tagcgttact acaaaaatta gggtgatgat gaaaaaaatg
     2401 atgaacaccc caatgtcaat tttattcttt tttacaagct tgaacatagc attattgttt
     2461 tgctccttgc caaatatacg gtagttatcc tcggagagta acgtgtcaaa cgtgtcaata
     2521 aatttttaag aaaacccttt acaacccgat ttatagatat ctttaaaatc cttacatttg
     2581 agacatcaaa agcagttcgg caatggattt acataaacag cttattgaag ccgttgagaa
     2641 gcgttttggt aaaaagatat cgtaccaacg cgactgtaaa accttgtcgg aaaaagtatt
     2701 tgagcataca cacgagtata taagcccggc aaccctccgc cggctttttg ggttcctgaa
     2761 aacgaattct aaaccaacac gtgtcacact cgatattctt gcacgctatt gcgggtatag
     2821 ccactggcaa gactttgttg aaaatcagat taaggcgagt gttacctcta aaacaaacat
     2881 accggagctt tggagtttaa ttaccacaaa tacactggag gtaaaccacc atagcattcg
     2941 ttcaatacaa ataaaatccg gaatcccatt tgaaaaaacc gtaaggcgaa catatctgga
     3001 atcgttgctt aatcactttg taagttcaaa ctacacagca ttggcaattg tggcgccagg
     3061 cggttatgga aaatctactt tcattgccca ctggtactta aataacccta aagtaaaaaa
     3121 acagcaaata atacccatca tcatttcggc atcaaaactt gaaaactatg cgttttcaga
     3181 gctaaatatc gatgaatggt tgtggggtca aattggcatt aacccatttt ctatcccaaa
     3241 tttaaaagat gagctcaaaa aactgcctgg ctcggttgta ttcataattg atgcccttga
     3301 tgaggttacc cttacaggca ctaaacttga aagaacactt aaaggaatac acgaactggc
     3361 caaagagttt tcagaatcgg ccaacaccaa gctgattatt acatcaaggt atgccacttg
     3421 gaaatcgctg cagggttatc ttataaaccc agaggtttgg ctgaacattg atcaatccaa
     3481 cttcaactcc gataacgcca atatccctcc cctttcgtac gatgaaatgc aaacagttct
     3541 tgataacact gtgaacgaag ggaaaaagga acgcttgctc atattcgagt taccgccctt
     3601 gctacgccaa accatttcgt acccatttta cctacaactt ttcattaaca gctacggaaa
     3661 ggcaacctac caaacccttt ccgacccact gaatatcctc aacgagttta tcaaacaaaa
     3721 agtatttgat tcaatttacc ctgaagagaa agccgatatt ttgaatgcca ttgccaaaga
     3781 atggttggaa aactctggtg caatcaccaa gcagcagctt aaaaagctct accccataaa
     3841 cctaaaaact gcaggcaact actttgacgc ttaccatgat ttactttcgt tcggaattat
     3901 ttctgaagag aaggtgtatt ccgattcact aggctactca actcttgttt ccataagcaa
     3961 taataatata ttaacatcgc ttattgcaca aaaactaatt aaggagtacg gctttaacga
     4021 gaaattaatc aatgcaacaa aaaatctctt ttcactaaac gagatttacc caaaattgat
     4081 tggatacata tttagcatta gctttaatga acgtaaggtt gcttttctta aggatttgtt
     4141 ccaactattt ttgcccgatt acatcacgga gctcaccaat accctcacgc tatgtttaaa
     4201 acgcgatgaa tcactattca aggatttgct tcctcacctt ctcaaaaata gagaattaga
     4261 gagggagtta ttgctcataa gtcccgattt gaatggcacc tcatcaacct ataggaaaat
     4321 actagaacaa gtgcaaccct caagccctta ctacaaggat tcaaaaatag caatggatta
     4381 ctgcaacctt ataggtttgg aaatctcgca aattaacaat agcaatgagt ggattaataa
     4441 ttttattctg actgagcaaa gttcagccag aattgcaggt atgtggttct gccttaatat
     4501 tttcttttca tctagttcaa aatccgaact aatggtaaag aaagctttag aattctataa
     4561 aagtatcaat tccgccaatg ctaagcttga atttagggag tcggttttac cgttagtttt
     4621 cattgataag cgctttttcc cacttcaaga tattttcata gagcccttaa atcactcggc
     4681 acaccaaaaa gcgttggact ctatcattta tgagtttacc gatatcacta aaaatcgtac
     4741 cccatttact ccggaaaagc atcatcaact tatgcaaaac tacagccagc tcaatcccag
     4801 taggaaccat tttgcaatag tactcggaga actagcacgc tcaatatcgt acttttacga
     4861 aagcaacctg aacgatgcac atacctgtat aaggaactct attgaacttt gtggaatatc
     4921 gggttaccgc ttacaggaaa tggtattgat gaacatactt ggtcaaaccc taatacgact
     4981 aggcgaaaca aactatggaa aatcgtttgt ttcatatgcc gaaactcttt ctgaaaaatc
     5041 gggtgttaag gtgtattctg agcattttgt gtgtggtagt taataagctc aactattggg
     5101 tagcgcaagt attgcgatat tttaaaccca tactcctcac caaccgaatc aagaacctga
     5161 ctaaaaagac atcctacgga accggttaca ccaattgata agtcggatag attctcaaaa
     5221 accgcaaagt ggtacctgta aagggctaca aacgattcct ttacaataga gtatatatgc
     5281 tggttatgta tattctcaag aataaaaggt acaaacgatg caaggtatgc cgatgggcgg
     5341 ggtgcactat aaaccatatt gagcacatgg cttagctcaa cattgtactg ttgctcaagt
     5401 ttttgggcta aaccaacatc aagtgttttg taatgatatg cacgaagcaa cagccgtccc
     5461 atgtaagctc cactaccctc gtcacccaga acaaatccaa gcgagggggt tcggttatta
     5521 agccgttcgc catcgtagta ggcaatattt gagccagttc caaggattac aacaataccg
     5581 gtttggcgct taaatagcgc ccgggctgcc cctaaagcat cggtaactgc atggattttg
     5641 gcgttgctaa aaaaaacacc cagatacctt gccacatcct gtcccctttc atgcactccg
     5701 catcctgaac cgtaaaagaa aatctccttt acctgttctg gattgatttg cttggggaaa
     5761 ttttgattta atgcgcttaa aaaatcttcc tgcctattaa agtatggact taacccaatg
     5821 gtttcgaaac ttgcaatggg cttgccgtgt tctatgattc tccaatcggt ttttgtagct
     5881 ccgctatcgg caataattat cattttatgc tttttacttt tacttttttc aaaactaaaa
     5941 aattcatcgg atttacacca ataccatcaa ctctggccga ggtaaaccta accactttat
     6001 cgtagtacaa aggtataact atggcttttg atgtaacaat agaatcgagg ctatggtagt
     6061 atggtaatcg ttggt
//
LOCUS       DAOSAL010000107         6060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_8278_length_6060_cov_54.245129, whole genome shotgun sequence.
ACCESSION   DAOSAL010000107 DAOSAL010000000
VERSION     DAOSAL010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 6060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6060
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8278_length_6060_cov_54.245129"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(510..1160)
                     /locus_tag="PK049_10750"
     CDS             complement(510..1160)
                     /locus_tag="PK049_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK62080.1"
                     /translation="MKKIIFIAFSLLILASCKSEKTEDLNRETVIMKEFLSQFFDVNS
                     RKFIILKTDQKLNQESEIYKFMGKNIKNGYQSFSFEALDETKGKIVVESATQNIDNKN
                     DSILLYTTYVNYKDQLGGNVTYLFIIEKTKDEKYMIKDFIKLDSVNKSLAPCNYSEAR
                     TYGECFSTCMGCMLDTNEFMGQAITGLGIVSGFGCGPCGVAAGVLFGIGTLACLGC"
     gene            1551..1952
                     /gene="ssrA"
                     /locus_tag="PK049_10755"
     tmRNA           1551..1952
                     /gene="ssrA"
                     /locus_tag="PK049_10755"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            2169..5543
                     /locus_tag="PK049_10760"
     CDS             2169..5543
                     /locus_tag="PK049_10760"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOK62081.1"
                     /translation="MSNFITNSNAKDLKERLSEIIGVSKELKFLVGFFYFSGISELYD
                     SLKANPDVELKILVGLDVDFLNRDLVEYAYGSNQSDEAKLNGFFNSIRKSINSDSFDS
                     KEFYEQVRFFINLIQNDKLLIRKTFNPNHSKLYLFKFNNHQIIRKNLFITGSSNLTRR
                     GISTQEEFNVEISDYGFSDAEKYFDELWERAVKINEVELAKKKLIEIIENETLIKEIT
                     PFEAYCLILKSFLDTFEHKSLKTYVIELLKKNGYKQYRYQTDAVSQALAIIENHNGVL
                     IADVVGLGKTIIACTIAKQLGKRGVVICPPGLMGDKNKTEGWTKYLEQFELYDWEVRS
                     VGDLESTLELVKGNPDFEVIIVDEAHRFRNQDTQNYELLMNICRGRKVILLTATPYNN
                     KPGDVLALLKLFITPKKSGITLDNNLEDKFQEIEIVFKKLGYIRKHYNSNDIDKRKRA
                     EYYYKQIFKQLPIDLSKVKAKAHMLARQIREIIEPVTIRRNRLDLIKNVNYRNEVNEL
                     SKVENPMEWFYGLTPDQLTFYDTVVNYFFAPPDEGGFFKGAIYQPYRYEKKTDIHLDE
                     EQQTLESHREQIQQQNLYDIMRRMLVKRFESSFGAFSKSIDNFISITEKVLEFIYKTG
                     QNDPLKGEYILDRDLMEKLLELSDDEVEEKLIEYETQIALGVYPRKHKRYRIQSFARG
                     REFIEDIMSDLELFKRISQKLNHLKLVENDPKAECLIEEISKIINDKPSPKEPKRKVI
                     IFSEYADTVKHLEERLGKINPTLAERTLVVSGNLPENTISVINKNFDASAKIQVNDYD
                     ILICTDKLSEGFNLNRAGMIVNYDIPWNPVRVIQRLGRINRISKKVFEKLYIVNFFPT
                     EKGAEYVRSREIAQGKMFIIHNTLGEDAKIFDIDEEPTPSGLYDKLTQNPENTDEESF
                     YTKVLNLFESVKNEHPQIVEKLNNMPIRVKVAKPFDQNKMFVFFRKNKLYVKSAFRDD
                     GKILVEDSSLELVLDQIACTPNTKPLHISDEFWSIYQAVKEIKEKSSSSIPATSIASK
                     ALNKIDLLLRGSTTNLPYNLRAFLVMLREDIIDYGTLPDYTLRRISNLSHSNTDKLVK
                     ELQTLCNELGENYLEAIKNSPEPLKEIIVAVYNTNE"
     gene            5585..>6060
                     /locus_tag="PK049_10765"
     CDS             5585..>6060
                     /locus_tag="PK049_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK62082.1"
                     /translation="MNSPKIHNLIESFSSESLIDFFREKTSSFTPKRDQISSDNPDFI
                     NGQLVGVFTTDNENFGRDNVAVFSFKTGRPLNERSGKKAQYEVAKKILKNNTGYTAGI
                     FVFYDGKGDFRFSLVYDIITDTAKRQWSNFRRFTYFVSREQTNKTFIRQISEAEFTS"
BASE COUNT         1986 a         1164 c         1158 g         1752 t
ORIGIN      
        1 cccgatgagt ggcacgatat atcgtttact ctaatcctgc ataacattga ggtaacccct
       61 gagcttacta tcagaacggg attctggaac aagcgtaagg gtgagttcct tattgacgac
      121 tatagggttg aggtaagggt aaaataacta gtctgatgcc tatttttctt gtatcggtat
      181 aaattttagc aaaaactttt aagccaaaaa ccatgtgcat ctgcagatgc acatggtttt
      241 tcaatctgac ttatttgtgg tgagtacctt tatattatta aaagcattat ttgtaaaatg
      301 cagaacaaca ttaaaaccac ttatttaata gatttttgat acttctaaag tagaacgcta
      361 taagtactcc cagaaagata atgacactga ttattttaat agtgacagaa tgggagttag
      421 caagtataac taacaatacc aaaaatagac caaaagttct tgtacctttc gaacctttca
      481 tgttacataa aaaattataa acaaaaatat tagcagccta aacaggcaag tgtgcctatt
      541 ccaaataaaa cgcctgcagc tacaccacat gggccgcaac caaaacccga tacaatacca
      601 agacccgtta ttgcttgtcc cataaattca ttcgtatcga gcatgcaacc catacatgta
      661 ctgaagcact caccataggt tcttgcctca gaataattac atggagccaa agacttgttg
      721 acagaatcaa gtttaataaa atccttaatc atgtattttt catccttagt tttctctatt
      781 ataaatagat atgtaacatt accaccaagt tggtccttgt agttaacata agtggtataa
      841 agcaaaatgc tatcattttt attatcaata ttttgcgttg ctgattctac tactatttta
      901 ccttttgttt catctaaagc ttcaaatgaa aatgattgat aaccattttt gatgtttttt
      961 cccatgaatt tataaatttc ggattcttga ttaagctttt ggtctgtctt taagattata
     1021 aattttcgac tatttacatc aaagaattgg gacaagaact ccttcataat tacagtttct
     1081 ctatttaagt cttctgtttt ttctgattta catgaagcta aaattaacaa agaaaacgca
     1141 ataaatatta tttttttcat ttttctagtt tattaaattg atcctactct tttcaaggtt
     1201 ttcggttccc cttttgatta attttggcca atactaatca tgtgcaaaaa aaaaattaaa
     1261 aaaaacataa aaaaaaaatt aacacaaatt aacacattaa tgtttaacat aaaaaattaa
     1321 aggagtcatt tccgattatt agtacgcagt tttaaaataa ttaaataaag tagatagtgg
     1381 taataggcag attgtcaatc tactagaatc aagtcaatca gtgttatctg tgttctatta
     1441 tttgactatc gctatacaat ttagcacaga caatttatga aacaaatatt atccatagtc
     1501 gtaaaataag tttacttaaa agcattatat ttgcagttca atcagtcaga ggggctgacg
     1561 tggttttgac agcaagtaga atgggtttgt aagcatgccg agcgtcgtga ggccggctcg
     1621 ttaatcccgg gcacaaacca ataaacggcg aaactaacta cgctctcgct gcctaataac
     1681 tccaggagtt aaggcttaat ccctacgcca aggatgcgtg gggacgagat gtcccgccgg
     1741 gaattacgcc ctgttgtttc cggcatatgg ggcaccgatc aaacggggat agtcggggcg
     1801 acacctttaa accccgatga aacaaacaaa ggttaaggat tggttcggtt gcctttcacc
     1861 tggccaatcc cgacaatcaa gaaaggctaa gcatgtagaa agcaagctgg ttccttgttt
     1921 ggacgagggt tcgattccct ccagctccac tttaacgcat tcattcgaat tcaaaacgcc
     1981 ctaaaatgta gatttttagg gcgtttttgt ttatgaacta tttgatattt taatatttta
     2041 ccttggcata aacaacagtt ctatagtttg tatttctgta tcttaaacat ttgtttaatc
     2101 aaagttttta taaacaatcc aaattagttt gaatcatttt gtatactttt ggaataaaat
     2161 tcagttttat gtcaaatttt attaccaata gtaatgcaaa agaccttaag gaaaggcttt
     2221 cggaaataat aggtgttagc aaagaactta aatttttggt ggggttcttt tacttttcag
     2281 gcattagtga actatatgat tcgctaaaag caaatcccga tgtggaactt aaaattctgg
     2341 tagggctcga tgtagatttt ctgaatcggg atttggtgga gtatgcatat gggagcaacc
     2401 aatccgacga ggcaaaactt aacggctttt tcaactccat tagaaagtcc attaacagcg
     2461 acagcttcga tagcaaggag ttttatgagc aggttagatt ttttataaat ctcattcaga
     2521 acgacaagct tttaattaga aaaacattca atcccaatca ctcaaagctt tatctattta
     2581 agtttaataa ccaccagatt attcggaaaa accttttcat tactggaagc agcaatttaa
     2641 cccgtagggg gattagcacc caggaagagt ttaatgttga gatttccgat tacggattta
     2701 gcgatgccga aaagtacttt gatgagctat gggaaagggc agtcaaaatc aatgaggttg
     2761 aactggccaa gaaaaaactt attgagatta ttgagaacga aacgctcata aaagagatta
     2821 ccccttttga ggcatactgt cttattctta aaagttttct cgacaccttt gagcataaaa
     2881 gtttgaaaac atacgttatt gaacttctta agaaaaatgg gtacaagcag tataggtatc
     2941 aaaccgatgc tgttagccag gcattagcaa tcatcgaaaa ccataatggc gttcttattg
     3001 ccgatgtggt agggcttggg aaaaccatta ttgcctgtac cattgccaaa cagttaggta
     3061 aacgaggcgt ggttatttgt cctccgggct taatgggcga caaaaacaag accgaaggat
     3121 ggacaaaata cttggaacag tttgaactgt acgactggga ggtacgctct gttggtgatt
     3181 tggagtcgac ccttgaactg gtgaaaggta accccgattt tgaggttatt attgtggacg
     3241 aggcccacag gttcaggaat caggataccc agaactatga attactgatg aacatctgta
     3301 ggggcagaaa ggtcatactg cttacggcca ctccatataa caataagccc ggcgatgtat
     3361 tggccctgct aaaactcttt ataactccca aaaaatcagg aattacactt gacaataacc
     3421 tggaagataa atttcaagaa atcgaaatcg tatttaaaaa gcttggctat atcaggaaac
     3481 actataattc taatgacata gataaaagaa aaagggccga atactactac aagcaaattt
     3541 ttaagcaact ccccatagac ctgtcaaagg ttaaggctaa agctcatatg cttgccaggc
     3601 aaattcgtga aattattgaa ccggtaacca taaggcgcaa caggctcgat ttgattaaga
     3661 acgtcaatta ccgtaacgag gttaatgaac tctcaaaggt ggaaaatccc atggagtggt
     3721 tctatggctt aacccctgat cagttaacat tttacgatac tgttgtaaac tacttctttg
     3781 caccccctga cgagggagga ttctttaagg gtgctattta ccaaccctac cgttacgaaa
     3841 aaaaaacgga catacacctt gatgaggagc aacaaacttt agaaagccac cgtgagcaaa
     3901 tccagcagca aaacctatac gatattatgc gccgtatgct ggtgaagcgt ttcgagagtt
     3961 catttggcgc atttagtaaa agtattgata atttcatttc aattaccgag aaggtgctgg
     4021 agttcatata taaaactggc cagaacgatc cccttaaggg tgagtatata cttgaccgcg
     4081 acctgatgga aaagctactc gaactatccg atgacgaagt ggaagaaaaa cttattgagt
     4141 atgagaccca aattgcccta ggggtttacc cgcgcaagca caagcgatac cgaatacaaa
     4201 gctttgcaag gggtagggag ttcattgagg atattatgtc cgatttggag ctattcaagc
     4261 gtattagtca gaaactcaac caccttaagt tagttgaaaa cgatcccaag gccgagtgcc
     4321 taattgagga aatatcaaaa attattaatg ataaaccaag tcctaaagag cctaaacgga
     4381 aagtaataat tttttcagag tacgccgata ccgttaagca tctggaagaa cgattaggga
     4441 agataaaccc aacccttgcc gaacgcaccc ttgtagtttc tggaaatttg cccgaaaaca
     4501 ctatttctgt cattaataag aattttgatg catcggctaa aattcaggtc aacgattacg
     4561 acattctaat atgtaccgat aagcttagcg aaggctttaa cctgaatcgg gcaggcatga
     4621 ttgtaaacta cgatatacca tggaatccgg ttcgcgttat tcagcgtttg ggacgtataa
     4681 accgcatcag taaaaaggtt tttgaaaagc tttacattgt aaactttttc cccactgaga
     4741 agggtgccga gtatgtccgt tcgcgtgaaa ttgcccaggg caaaatgttt attatccaca
     4801 atacccttgg cgaggatgcc aaaatctttg atatcgacga ggaacctacc ccatcggggc
     4861 tttacgataa gctcacccaa aaccccgaga ataccgatga agaaagcttt tacaccaagg
     4921 ttcttaacct gtttgagtca gttaaaaatg aacatcccca aatagttgaa aaactaaaca
     4981 acatgcccat tcgggttaag gtggctaaac cattcgacca aaacaaaatg tttgtattct
     5041 tcaggaaaaa taaactttac gtaaaatcgg catttaggga cgatggaaag attcttgtgg
     5101 aggactcctc gctcgaattg gttttagacc aaattgcatg cacacccaat accaaaccgc
     5161 tgcatatttc cgatgagttt tggagtattt atcaggctgt aaaggaaatc aaggagaaaa
     5221 gcagttctag cattcccgct acaagcattg cctcaaaagc cttgaacaaa attgatctat
     5281 tgctacgcgg ttcaaccaca aaccttccgt ataatcttcg cgcatttttg gtcatgctcc
     5341 gggaagatat tatcgactac ggtactctac cagattatac ccttcgccgt atatcgaacc
     5401 ttagtcattc caataccgat aagcttgtaa aggaactcca aaccctttgc aatgagctgg
     5461 gcgagaacta ccttgaggct ataaagaata gccccgaacc cttgaaggag attattgtgg
     5521 ctgtttataa tacaaatgag taaacacaca ttgctaaata gataaaaact gatttgcttt
     5581 tgttatgaac tcacctaaaa tccataacct cattgagagt ttttcatccg agagtctaat
     5641 tgactttttc cgtgaaaaaa catcttcatt tactcctaag cgcgatcaaa tttccagcga
     5701 caaccccgat tttatcaatg gccagctggt tggggtattt accaccgaca acgaaaattt
     5761 tggtagggat aatgttgctg tattcagttt taaaacgggt aggccactga atgagcgaag
     5821 cggtaaaaag gctcagtatg aggtggcaaa aaaaatactc aagaacaaca caggctacac
     5881 tgcaggtatt tttgtttttt acgatggtaa gggcgatttc cgattctcct tggtttacga
     5941 cattataacc gataccgcaa agcgtcagtg gagcaacttc agacgcttta cctattttgt
     6001 cagccgcgag caaaccaaca aaacctttat ccgtcagatc agcgaggctg aatttacctc
//
LOCUS       DAOSAL010000108         5984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_8413_length_5984_cov_46.501602, whole genome shotgun sequence.
ACCESSION   DAOSAL010000108 DAOSAL010000000
VERSION     DAOSAL010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 5984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5984
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8413_length_5984_cov_46.501602"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..970)
                     /locus_tag="PK049_10770"
     CDS             complement(83..970)
                     /locus_tag="PK049_10770"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOK62083.1"
                     /translation="MRIEIDPNAGFCFGVVNAIKTAEENLSKGIEINCLGEIVHNEME
                     NNRLKGIGMKTVKHDQLPELGGRSVLIRAHGEPPSTYELAKKYNINLVDATCPVVLKL
                     QERVRKAWERLQPLGGTVIIFGKKGHAEVIGLCGQTNNQAVVVQDLNDLDGIDFSKPI
                     EIFSQTTKEPEHYREIVEEIKRRVAIYNTQPETLLKVNNTICGQVSNRKPHIKAFAQK
                     HDLMIFVSGTNSSNGKMLFEACLEVNPNSHLIVNSAELKPEWLSGCQSVGISGATSTP
                     LWLMQEVASAIEAIVKGKG"
     gene            complement(1016..1705)
                     /gene="cmk"
                     /locus_tag="PK049_10775"
     CDS             complement(1016..1705)
                     /gene="cmk"
                     /locus_tag="PK049_10775"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOK62084.1"
                     /translation="MDKKIVIAIDGFSSCGKSTFAKAIAKRLGYLYIDSGAMYRVVTL
                     ELLRRGLINDGVVDTSMLQSILKEINIGFSYDPVKMAYLTTLNGEVVEDEIRSPQVAN
                     HVSLVAAIPAVRTRLVELQRELGKKKGIVMDGRDIGTVVFPDAELKIFMTADPIVRAR
                     RRLKELQEKGLNVTLDEVLKNIEERDYIDQNRDVAPLRKADDAVVLDNTNMTVEEQME
                     WVEEMVKRLTV"
     gene            complement(1768..2802)
                     /gene="porQ"
                     /locus_tag="PK049_10780"
     CDS             complement(1768..2802)
                     /gene="porQ"
                     /locus_tag="PK049_10780"
                     /inference="COORDINATES: protein motif:HMM:NF033711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HOK62085.1"
                     /translation="MRFATTIILCFIMAISVNGQNGGLSTYQFLNLPYSSRVAAFGGK
                     MAASDLADISLTNQNPAMLDSSLHNAVSLFYTSYYASINFAAVSYAQRLNSRSTLGFS
                     IFGINYGRFDQADESGAISGTFGGNDFALAVTYSYRIDSCFNVGVSVKPIYSHLERYY
                     SLGISTDFGVHYLSGNKLFAAGIALRNVGVMVKPYTANTYENLPFEAVAGFSKKLAHA
                     PFRFVVTMQHLERYDLYYSSPINKTDILGEDDKPSPNVVERVGRELISHLIAGIEFVP
                     VKGFALRLGYNYQRRNELKVQERVSTVGFSWGVSLRIKKFDVSYSRATYHLTGSTNHF
                     SITTNLQNWL"
     gene            complement(2869..3858)
                     /locus_tag="PK049_10785"
     CDS             complement(2869..3858)
                     /locus_tag="PK049_10785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HOK62086.1"
                     /translation="METSWFVVVNPRAGGGKSVVDWPIISRLLNKYDIHYSHAFTEYK
                     YHAVELTVQAIVKGYRNIIAVGGDGTLNEVVNGIFIQKTVSPSDITVGVIGVGTGNDW
                     FRMYEIPHNYEECVRAIKNKRVFKQDVGVAEYYKSSVRHTRYFINAAGVGFDAEVARR
                     TNRLKDLGKRGALLYILSLIKALIRYRSTRINVKVDGKHFQHTVFSLTIGICRFNGAG
                     MMQVPFALSDDGLFDVTIIKKISKLNVVANLPRLYNGTILKHSRVLGVRGKSIEIMSI
                     PAVGLEVDGESLGETPLKFSIMQQALSVIVGEQFNQSKLTAKKQPQLLEPAAR"
     gene            4102..4902
                     /locus_tag="PK049_10790"
     CDS             4102..4902
                     /locus_tag="PK049_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOK62087.1"
                     /translation="MAKVIALANQKGGVGKTTTAINLAASLAVLEKKVLLIDADPQAN
                     ATSGTGFDLRNVKTSIYECLIDDINPKDIVLNSEIKGLDLIPSHIDLVGAEIEMLNMP
                     NREKILKGVIEKVKDDYDFVFIDCSPSLGLITVNALTAADSVIIPVQCEYFALEGLGK
                     LLNTVKIIQTRLNTELQIEGFLLTMYDPRLRLSNQVVDEVRKHFQQMVFETIIQRNIK
                     LSEAPSYGKPVILYDAASTGSLNYMNLARELLQKNNMTSIDNSSKIEE"
     gene            4922..5833
                     /locus_tag="PK049_10795"
     CDS             4922..5833
                     /locus_tag="PK049_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOK62088.1"
                     /translation="MSKKMALGKGLGALIDDAVDPTRNQDIKTEKQVVSELVNEIEIE
                     KIDVNPYQPRTQFDEEALKELAESISKLGVIQPLTVRAVDGRFQLISGERRLRAAKLA
                     GLKTIPVFIRTADDQGMLEMALVENIQREDLNAIEVAISYQRLIEECNLTQETLSERV
                     GKKRATISNYLRLLKLPAEIQLGISQDLISMGHARALITIEDPKVQLDIYHQTIAEGL
                     SVRRVEELARMANDPTPVSPAKTKDGKEKVNLGEQEIVLKDHLTNRLGLRADVKPGPK
                     GDGKIVISYNNQAELEAILEKFIKIEE"
BASE COUNT         1688 a         1445 c         1253 g         1598 t
ORIGIN      
        1 acgggtcacc ctttatcctt tatcctttat cctttaccct ttatccttta acctttatcc
       61 tttaaccttt atcctttaac ctttatcctt tccccttaac aattgcctca atggcacttg
      121 ccacctcctg catgagccaa agtggggttg aggttgcccc gctaatacct acactctggc
      181 aaccacttaa ccattctggc tttaattcag ctgaattcac aatcaaatgg ctattggggt
      241 ttacttccag gcaggcctca aaaagcatct tcccgtttga gctgttggtg ccactaacaa
      301 aaatcatcaa atcgtgtttt tgtgcaaaag ccttaatgtg aggcttccgg ttcgacacct
      361 gtccgcaaat ggtattatta accttaagta gggtttcggg ctgtgtgttg tatatagcta
      421 cccggcgctt tatctcctca acaatctccc tatagtgctc gggttccttt gtggtttgcg
      481 agaaaatttc gataggttta ctgaaatcga tcccgtcaag atcgttcaaa tcctgaacta
      541 ctacagcctg gttgttagtt tgtccgcata ggccgataac ctcagcgtgc ccctttttac
      601 caaaaataat tacagttccg ccaaggggtt gaaggcgttc ccaggccttg cggacacgtt
      661 cctgcagctt aagaaccacc ggacaggtag catcaaccag gttgatgttg tacttttttg
      721 ctaactcata ggttgatggt ggttcaccgt gagcacggat taggacactc ctccctccca
      781 actcgggcaa ctggtcgtgc tttacggttt tcatgcctat ccctttaagg cgattatttt
      841 ccatctcgtt atggacaatc tcgcccagac agttgatttc aattcctttg ctcagattct
      901 cttcggctgt tttgattgca tttactacac caaaacagaa accggcatta ggatcaatct
      961 caattctcat tcaaattcag tattatactg ctaaacagat aagctataat ttggtttaaa
     1021 cggtaaggcg cttaaccatt tcctccaccc actccatttg ctcctcaact gtcatgttag
     1081 tattatcgag aaccacggca tcatcggctt tgcggagtgg agccacatcg cggttctggt
     1141 caatgtaatc gcgctcctca atatttttaa gcacctcgtc taaggtaaca ttcaaaccct
     1201 tttcctgcaa ctcctttaac ctgcgccggg cacgaacaat agggtcggct gtcatgaata
     1261 ttttcagctc agcatcgggg aataccactg taccaatatc gcggccatcc attacaattc
     1321 ctttcttttt gccaagttcc ctttgcaact ccactagtcg ggttcgcaca gccggtatag
     1381 cggcaacaag gctcacatgg ttagcaacct gcggactccg aatttcgtcc tcaaccacct
     1441 cgccattcag ggttgtaagg taagccattt taacaggatc gtatgagaag ccaatgttta
     1501 tctcctttaa aatactttga agcattgatg tatccaccac gccatcgtta ataagccccc
     1561 ggcggagcag ttcaagcgta accacgcgat acatggctcc gctatcaatg tacaggtatc
     1621 ctaaacgttt tgcaatggcc ttagcaaacg tgcttttccc acacgacgag aatccatcaa
     1681 tagcaataac tattttttta tccattggtt gtaagatcat tttttgaacc cgctaaggta
     1741 ataaaaactc cacttgatgg gtggcctcta aagccagttt tgaagattgg tggttattga
     1801 aaaatggttg gtagagccgg ttaaatggta tgttgcccta ctgtagctaa catcaaactt
     1861 ctttattcta aggcttactc cccagctaaa cccaacggtt gaaactcgct cctgcacctt
     1921 aagttcattc cgacgctggt agttataccc tagacgtaag gcaaagcctt tcacgggtac
     1981 aaactctatc ccggcaatca aatggctaat caactcacgg cctacccgct caaccacatt
     2041 gggcgacggt ttgtcatctt cgccaagaat atcggttttg ttaatggggg aactgtaata
     2101 caaatcgtaa cgctccaggt gctgcatggt tacaacaaac ctgaaaggtg catgcgccaa
     2161 cttcttactg aaaccggcaa cagcctcaaa aggtaagttc tcgtaagtat tggcagtgta
     2221 aggttttacc atcacaccaa cattcctaag cgcaataccc gcagcaaaga gtttattgcc
     2281 tgaaaggtaa tgtacaccaa aatcggtaga aatccccagg gagtaataac gctccaagtg
     2341 cgagtagatg ggtttaacgc ttacccctac attaaaacag gaatcgatac ggtagctgta
     2401 ggttaccgcc aaagcaaaat cgttgccgcc aaaggtgccc gatattgctc ccgattcatc
     2461 ggcttggtca aaacggccat agttaattcc aaaaattgaa aagccaagcg tacttctgga
     2521 gttcaatcgc tgggcgtacg acaccgctgc aaaattaatg ctggcgtagt agcttgtgta
     2581 gaacaagcta acggcattat gcagtgatga atcgagcata gccggatttt ggttagtcag
     2641 ggagatatcg gccaaatccg atgctgccat cttaccacca aaggcagcaa cgcgcgaaga
     2701 gtaaggcaga ttcagaaact gataggtaga taagcccccg ttctgaccat taacggaaat
     2761 ggccataatg aaacaaagaa tgattgttgt tgcaaaccgc atggtggttt tatgggctaa
     2821 aatctaatta aatatcattc taaacggata gtatatgcca aatattgcct acctggctgc
     2881 cggttcaagt agctggggct gtttcttagc ggttagcttg gactggttaa actgctcacc
     2941 aactataacg gagagcgctt gctgcataat tgagaattta agaggagttt cgccaagcga
     3001 ttcgccatca acctcaagac ctacggctgg aatactcatt atctcaatgc ttttccccct
     3061 cacgcctaaa acacgtgaat gctttaggat ggttccgttg taaagccgtg gcaggttggc
     3121 aaccacatta agcttgctta tctttttaat aatggtaaca tcaaacagcc catcgtccga
     3181 aagggcaaaa ggaacctgca tcattccagc gccgttaaag cggcaaattc caatggtgag
     3241 gctgaaaact gtatgctgaa agtgcttacc atcaacctta acatttattc gggttgagcg
     3301 gtaccggata agcgccttaa taaggctaag aatgtatagc aacgcgccac gtttccccaa
     3361 atcctttaaa cggttagtcc ggcgagccac ctcggcatcg aaaccaacgc cagcggcgtt
     3421 aatgaagtaa cgggtatgcc taacgctcga tttgtagtac tcggccacac ctacatcctg
     3481 cttgaatacc cttttattct ttattgccct aacgcattcc tcgtagttgt gtggaatctc
     3541 atacatccga aaccaatcgt ttccagtacc aacacctatt accccaacag taatatcgct
     3601 ggggcttacc gtcttttgaa taaaaatccc gttaaccacc tcgttcaggg taccatcgcc
     3661 accaactgca ataatatttc ggtagccctt tactattgcc tgcacggtga gctcaaccgc
     3721 atggtactta tactccgtaa aggcatggga atagtgaata tcgtacttgt tgagcaaacg
     3781 actaatgata ggccaatcga caaccgattt accccctcca gcccgcgggt ttaccactac
     3841 aaaccaactt gtttccaacg tatagaaatt tagtcatcga atccgatgca aatataacca
     3901 ttctgttaaa attcattcac ttatccggaa aatggttttt gtttgataaa agaatttcta
     3961 tttttgcagt taccgtttgc acaaaccgat tgttaataac ttgttaataa catcggggca
     4021 aatgttcata ttcacatcca tgggggtgtg gaatgaaaag gaaaggaaac ttatttttga
     4081 cccttgtaaa aaaacatagt aatggcaaaa gtaattgcat tagctaacca gaaaggtggt
     4141 gtaggtaaaa ccaccacagc tattaacctt gcagccagct tggcggttct tgagaaaaag
     4201 gtactcctga ttgatgccga ccctcaggcc aacgccacat cgggtaccgg ttttgatttg
     4261 cgcaacgtaa aaaccagtat ctacgagtgc cttatagatg atatcaaccc taaggatatc
     4321 gttctcaact ctgaaattaa ggggttagac cttatcccct cgcacatcga cctggttggt
     4381 gccgaaattg aaatgctcaa catgcccaat agggaaaaga tacttaaagg ggtaattgaa
     4441 aaggttaagg atgattacga ctttgttttc attgattgct cgccctcgtt gggactaata
     4501 actgtaaacg cactcactgc tgccgattcg gtaattattc ctgtgcagtg cgagtacttt
     4561 gcactcgagg ggttgggcaa gctgcttaat accgtaaaaa tcattcaaac ccgtttaaac
     4621 accgaactgc aaattgaagg attcctgcta accatgtacg atcctaggct tcgcctttcg
     4681 aaccaagtag ttgatgaggt acgcaagcac ttccagcaaa tggtgttcga gactatcatc
     4741 cagcgcaaca ttaagctgag cgaagcacct agctacggca aaccagtaat tctgtacgat
     4801 gctgcatcta ccggatcgtt aaactacatg aacctggctc gtgagctgct gcagaaaaac
     4861 aacatgacca gcattgacaa tagtagcaag atagaggaat aagaaggagg aaatatttac
     4921 catgtcaaaa aagatggctt taggcaaagg gttaggtgct cttattgacg atgcggttga
     4981 cccaacacgc aaccaggata ttaaaaccga aaaacaggtt gtgagcgagc tggttaacga
     5041 gattgaaatt gaaaaaatcg acgttaaccc ctaccaaccc cgtacccagt ttgatgagga
     5101 agcgctaaag gaactagcgg aatcaatatc caagcttggt gttatacaac ccctcacagt
     5161 tcgcgctgta gatggtcgct ttcagcttat ttcgggtgaa cgccgcctaa gggcagcaaa
     5221 gcttgccggg ctaaaaacaa tcccagtatt cattcgcact gccgacgacc agggtatgct
     5281 tgagatggcc ctggttgaaa acatccagcg tgaggacctt aacgccattg aggtagccat
     5341 tagctaccag cgcctgattg aggagtgtaa ccttacccag gaaaccctat ccgagagggt
     5401 tggcaagaaa agggctacca tatccaacta cttgcgcctg cttaaacttc cagccgaaat
     5461 tcaacttggc ataagccagg acctaattag catggggcat gctcgcgcac ttattaccat
     5521 tgaagatcct aaggtacagc tcgatattta ccaccaaacc attgccgaag ggctatcggt
     5581 tcgtagggtt gaagagctag cccgcatggc taacgatcca actcccgtaa gtcctgctaa
     5641 aactaaggat ggaaaggaaa aggttaacct tggtgaacag gaaatagtcc tgaaggatca
     5701 tcttaccaac cgcttgggac tccgcgccga tgttaagccc ggtcccaagg gcgatggcaa
     5761 aattgttatt tcttacaata accaggctga actggaagcc attcttgaaa agtttatcaa
     5821 gattgaggag tagatactag tgttggaatc catgcatggc acttgctttt tactaaatgg
     5881 ccgacaagtc aataaatgcc tgtcggcctt tgcaattttt tttcttttac ttaacgcacc
     5941 aagggtttac tcgcaaacaa gcgataccaa aacgctaaaa aagc
//
LOCUS       DAOSAL010000109         5969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_8437_length_5969_cov_45.287284, whole genome shotgun sequence.
ACCESSION   DAOSAL010000109 DAOSAL010000000
VERSION     DAOSAL010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 5969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5969
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8437_length_5969_cov_45.287284"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..1617
                     /gene="guaA"
                     /locus_tag="PK049_10800"
     CDS             88..1617
                     /gene="guaA"
                     /locus_tag="PK049_10800"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015532441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOK62089.1"
                     /translation="MHQKILILDFGSQYTQLIARRIREFNVYCEIHPFNRIPKLDNTV
                     KGVILSGSPFSVRDNNAPMPDLTDIRGRYPLLGICYGAQLLSHTNGGEVLPSAIREYG
                     RATLEVVDSNCPLFTSLSPKSQVWMSHGDTIAKIPDNFKVVASTADVKVGAFSVLGEQ
                     TYGIQFHPEVYHSVEGSKLLKNFVVDICGCNQDWTPASFIDETVSNLRNQLGNDKVVL
                     GLSGGVDSSVAAMLLHKAVGPNLTCIFVDNGLLRKNEFSQVLESYKTLGLNVIGVDAS
                     QRFYSELKGVSDPEQKRKIIGRIFIEVFDEEAHKIKDVKWLGQGTIYPDVIESVSVNG
                     PSATIKSHHNVGGLPEKMNLKVIEPLRLLFKDEVRRVGKELGLPGGILNRHPFPGPGL
                     GIRILGEVTPEKVRLLQEADSIYIQGLREAGLYDKIWQAGAILLPVQSVGVMGDERTY
                     EFVVALRAVTSTDGMTADWFHFPHEFLARISNEIINRVKGINRVVYDISSKPPATIEW
                     E"
     gene            1634..3226
                     /locus_tag="PK049_10805"
     CDS             1634..3226
                     /locus_tag="PK049_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOK62090.1"
                     /translation="MKKLLSIFLLVFALFFSAGAQNNSSFSAYKFVRFIQYLSGNYVD
                     TINVNQLVDDAIIEILGQLDPHSIYISKEDVQAMNEPLEGNFEGIGVEFNILNDTLIV
                     VNPISGGPSERVGIMAGDRIVEIDGKPITNIGLKIPDVHKLLRGPKGTRVDVKILRKG
                     VSQLLDFTIIRDKIPIYSIDASYMARPGIGYIKINRFAATTEQEFVEALEKLEKEKMA
                     DLIIDLRNNGGGFLNSAYELASYLLEPGKLVVYTQGRNSPRTDYFSHFRGRKPFKGRL
                     VIITDEGSASASEILAGAIQDWDRGIVVGRRTFGKGLVQNQLPLPDGSMIRLTVAKYF
                     TPTGRAIQKPYKMGDVKDYYSDLDNRYSRGEFFNQDSVHFPDSLKYTTLVKKKVVYGG
                     GGIMPDVFVPLDTTYYSEYYGKLVRSGLINQFAITWVDANRELLKKKYPKFEVYNKNF
                     EVTDNILDELYSYAESRQIARDSTGIQTSKAELKLQLKGLFAQSLFGSGFYYQVTNEH
                     NESFIKSLEVINNWTRYKHLVQ"
     gene            3301..4878
                     /locus_tag="PK049_10810"
     CDS             3301..4878
                     /locus_tag="PK049_10810"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOK62091.1"
                     /translation="MEINNEFRQLGLSEKALLAIKKKGFEQPSPIQKITIPVLLSESC
                     DIIAQAQTGTGKTAAFGLPIIDLLDGQPKGIKALVLVPTRELALQVCDEMLSLADKRI
                     IITAVYGGQSISEQQRRIKKGVDVVVGTPGRILDLINRGDLDFSQLQIFVLDEADEML
                     NMGFIEDIEEIMRHTPKEKRVLLFSATMPDRIVKLAKNYMKEPKRLEVDKAKPTTGLT
                     DQIYFEVREGDKFDALTRIIDIEPEFYGLVFCRTRNDVDVVTGKLIERGYAAEGLHGE
                     ISQAQREKTLSRFRKKHINILIATDVAARGIDIVDLTHVINYSLPQDPESYIHRIGRT
                     GRAGKQGTAITFVSPEEYRKLNYFQKATKVNIRKELLPTAEDIVAVKREKIKEELVEI
                     INQGNYMDYLGMADDLIETNSPEVALAALLKLAFKSQLDVSSYTEIRTFRVDTKGTTR
                     LFIGLGKKDGMTPKKISEFIRKEVKIPDRNIDDILVRDDFSFITVPFTDAEKILSAFS
                     NRTQDGKPLVTKAKPKK"
     gene            4893..>5969
                     /locus_tag="PK049_10815"
     CDS             4893..>5969
                     /locus_tag="PK049_10815"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HOK62092.1"
                     /translation="MSEKFQMVAKTLQGLEEVLAEELKEIGAENIEVGCRSVSYYGTK
                     ETLYKSNFWLRTALRVLKPIYVFNARTIDEFYGNARKFDWTSVMELSHKFAVESVVNS
                     ELFPHSRYIALKLKDAIADQFRDRFGKRPFVDPKKPHITFHIHVNNDVCTVSLDSSGE
                     SLHRRGYRVAQDVAPINEVLAAGLIKLSGWNGDSVFIDPMCGSGTILIEAALIAYGIA
                     PGIFRQHFGFENWLDFDEQLLQSIYNDDSRERDFEYLILGRDISKQAIASAMENIKES
                     GLNKKIDLGVASIFDYNPPRVDKGIVITNPPYGERLKKEQIENFYKQLSDVFKNKYAG
                     YDVWIISSNQEALRSFGLRPSKKIN"
BASE COUNT         1704 a         1216 c         1385 g         1664 t
ORIGIN      
        1 taatcataaa tattaaaacc gataagcaaa tcggccaata atttgttcta ttttcttacc
       61 tttgcaattc aaagtgtttg tgttaccatg catcaaaaaa ttttgatact cgattttggc
      121 tcccagtata cccagctaat agcacgtaga attagagagt tcaatgttta ctgcgagatt
      181 cacccattta accgaatccc aaagctcgac aataccgtta agggggttat actatccgga
      241 agcccttttt ccgtgaggga taataatgct ccaatgcccg atttgactga tattaggggt
      301 agataccctt tgcttggaat ttgctacgga gcacagttac tatcccatac caatgggggt
      361 gaggttttac catcggccat aagggagtac ggccgggcta ctttggaggt tgttgatagt
      421 aactgcccgc ttttcacgag cctatcgcca aaatcgcagg tttggatgtc gcatggcgat
      481 accatagcaa aaattcctga taactttaaa gttgttgcca gcactgccga tgtaaaagta
      541 ggcgcattta gcgtattggg cgagcaaacc tacggtattc agtttcaccc ggaggtgtat
      601 cacagcgttg agggatcaaa gctactcaaa aattttgtag ttgatatttg tggctgcaac
      661 caggattgga cacccgcatc gtttatcgat gaaacagtat cgaatctcag aaaccagtta
      721 ggtaatgata aggttgttct gggcctttct ggtggggtgg actcatcggt tgccgctatg
      781 ctgttgcata aagctgtagg cccaaatttg acgtgtatat ttgttgataa tggactactt
      841 cgtaaaaacg agttttcgca ggtattggag tcctacaaaa ctttgggtct caatgtaatt
      901 ggcgttgacg ccagtcaacg attctatagt gagctaaagg gtgtttccga cccggaacag
      961 aaacggaaaa ttattgggcg aatcttcatt gaggtttttg acgaggaagc gcataagatt
     1021 aaggatgtaa aatggcttgg tcagggaact atctaccccg atgtaattga atcggtatcg
     1081 gttaatggcc catcagccac cataaaatcg caccataacg tgggcgggct acccgaaaaa
     1141 atgaacctaa aggtaattga gcctttacgc ctacttttta aggatgaggt tcgcagggtt
     1201 ggcaaggagt tgggtttacc cgggggtatc cttaatcgcc atccttttcc cgggcctggt
     1261 ctggggatta gaatacttgg cgaagttaca ccagaaaaag tacgactctt gcaggaggcc
     1321 gatagcattt acatacaagg attacgcgaa gcaggtttgt acgataagat ttggcaggcc
     1381 ggagccattc tacttcccgt ccagtcagtg ggtgtaatgg gcgatgaacg cacatatgaa
     1441 tttgtagtag ccttacgtgc tgttacctct actgatggaa tgaccgctga ttggttccat
     1501 ttccctcacg aattcctggc ccgtatctca aacgagatta ttaatagggt aaaaggcatt
     1561 aatagagtgg tttacgatat cagctcaaaa ccccccgcca caatagagtg ggagtaaaat
     1621 tttattactg tgtatgaaaa aattattgtc aatttttctt ttagtttttg ccctgttttt
     1681 ctcggcaggc gctcaaaaca attctagctt tagcgcatat aaatttgttc gctttattca
     1741 gtacctatca ggtaactacg ttgataccat caacgtaaat cagctggttg acgatgccat
     1801 tattgaaatt ctgggacagc tcgatcccca ttcaatctat atctcaaagg aagatgttca
     1861 ggccatgaac gaaccccttg agggaaactt tgagggaatt ggtgttgagt ttaacatcct
     1921 taacgatacc ttaatagtag ttaatcccat ttccggaggt ccatcggaac gtgttggtat
     1981 tatggccggt gacaggatag ttgaaatcga cgggaagcca attaccaata ttgggttaaa
     2041 aatccccgat gtccataagc tactgcgtgg gcccaaaggt accagggttg atgtgaaaat
     2101 tttacgtaag ggtgtttccc agctactaga ttttaccata atccgcgata aaatacccat
     2161 ttacagtatt gatgcttcat acatggctcg tccgggtatt gggtatatca aaataaatcg
     2221 atttgctgcc acaaccgagc aggagtttgt tgaggctctt gagaaacttg agaaggagaa
     2281 aatggccgac ttaataatcg atttaaggaa caatggagga ggcttcctta actcggcata
     2341 cgagctggca agctaccttc ttgaacccgg taagctagtg gtttataccc agggccgaaa
     2401 tagcccccga accgactact tctcccattt ccgtggccgt aagccattta agggccggct
     2461 tgtgataata accgacgagg gttcagcctc cgcaagcgag attcttgctg gtgcaattca
     2521 ggactgggat cgtggtatag ttgttggtcg aagaactttt ggcaaagggc ttgtccagaa
     2581 tcaacttccc ttgcccgatg gttccatgat taggttaacc gtggccaaat actttacacc
     2641 taccggccga gccatccaga aaccatacaa aatgggtgat gtaaaggatt actattccga
     2701 tttggataat cggtatagta gaggtgagtt tttcaatcag gatagtgtcc attttcccga
     2761 ttcgcttaag tacaccactc tggttaaaaa gaaggtggtt tatggtggcg gtggcataat
     2821 gcccgatgtt tttgttccac tcgatacaac ttactactct gaatactacg gtaaacttgt
     2881 tcgatcaggt ttaattaacc agtttgctat aacctgggtt gatgctaacc gtgaattgct
     2941 taagaagaaa tacccgaagt ttgaagtcta caacaaaaac tttgaggtta ccgataacat
     3001 attggatgaa ctctattcct atgccgaatc ccggcaaata gctcgcgata gcactggtat
     3061 tcaaacttcc aaagctgaac taaaactcca gctgaaaggt ttgtttgcac aatcgctttt
     3121 tggttcaggt ttttactatc aggttaccaa tgaacacaac gaatcgttca ttaaaagcct
     3181 tgaggtgatt aacaattgga cacgatataa gcatttagta caataatttt tcagtttttt
     3241 ttttaaattt aagtgtttta atacttttca caggtcgttt tttgaccaat taattatgtt
     3301 atggagataa ataatgaatt caggcagttg ggtttatcgg aaaaagccct tctagcaatt
     3361 aagaaaaagg gttttgagca accatcgcct attcagaaaa taaccattcc ggtattgctg
     3421 agcgaaagct gcgatataat agcccaagct caaacaggta ctggtaaaac agctgccttt
     3481 ggcttaccaa taatcgattt actcgatggt caacctaagg gaattaaggc attagtcctt
     3541 gtaccaaccc gcgagctcgc acttcaggtt tgcgacgaga tgctatcgct ggccgataag
     3601 cgaattatta ttaccgctgt ttacggtggg caatcaatct cggagcaaca gcgccgtata
     3661 aaaaaaggtg tagatgtggt ggttggtaca cccggtcgca ttctcgacct aatcaaccgt
     3721 ggcgatctcg actttagtca gctccagata tttgttctcg atgaggccga tgaaatgctt
     3781 aacatgggtt tcattgagga tattgaggag attatgcgtc acactcccaa ggagaaaagg
     3841 gtactgcttt tctcggccac aatgcccgat cgtattgtga agctagctaa aaactacatg
     3901 aaggagccca agaggcttga ggttgacaag gcaaaaccca ccactggctt aacggatcag
     3961 atttactttg aggtgcgcga aggcgataag tttgatgctc ttacccgaat tattgatatt
     4021 gagcccgaat tttacggact ggtgttctgt cgtacccgca acgatgtgga tgtagttacc
     4081 ggaaagctaa ttgaacgggg ttatgctgct gagggattac atggtgaaat ttcgcaggca
     4141 cagcgtgaaa aaaccttgag ccgtttccgt aaaaagcata taaatattct tattgctacc
     4201 gatgtggctg cacggggtat cgacattgtt gatttaaccc atgtgattaa ctacagcctt
     4261 ccacaggatc ccgaatcgta cattcaccgc ataggacgta ccggacgtgc cggaaagcag
     4321 ggaacagcta ttacatttgt ttcgcccgag gaatatcgta agctgaacta tttccagaaa
     4381 gccacaaagg ttaatattcg caaagagttg cttccaacgg ctgaggatat tgttgctgtt
     4441 aagcgcgaaa agattaaaga ggagttagtt gagattataa accagggcaa ctatatggat
     4501 tatcttggaa tggctgatga tcttattgag accaacagtc cagaggtagc cctggcagcc
     4561 ctgttaaagc ttgccttcaa aagccagctc gatgtatcga gttacactga aattagaacc
     4621 ttccgtgtcg ataccaaggg aacaacacgc ttgtttattg gacttggcaa aaaggatggt
     4681 atgactccca agaagatttc agagtttatt cgtaaagagg tgaaaatacc cgataggaat
     4741 attgacgata tactggttcg cgatgacttt tcgttcatca cagtaccgtt tactgatgcc
     4801 gagaaaattc tctcagcatt ctcaaacaga acccaggatg gtaaaccttt ggtaacaaaa
     4861 gcaaaaccca agaagtagat agcttagtaa tcatgagcga gaaatttcaa atggtagcca
     4921 aaacccttca gggtttggaa gaagtattgg ccgaagagct aaaggaaatt ggagctgaaa
     4981 acattgaggt tggttgccgt tcggtttcct attacggaac caaggagact ctctataaat
     5041 caaatttttg gctaagaacc gctctgcgag ttctaaaacc catttatgtt ttcaacgccc
     5101 gtacaatcga tgagttctat gggaatgccc gcaagttcga ttggacttca gtaatggagc
     5161 tttcccacaa atttgccgtg gagagcgttg tgaactcgga gttattccca cattcacggt
     5221 acattgcttt aaaactgaaa gatgcaattg ccgaccagtt ccgcgatagg tttggcaagc
     5281 gtccttttgt cgaccccaaa aaacctcaca tcacctttca tatccatgtg aataacgatg
     5341 tttgtaccgt atcgctcgat tcatcgggcg agtcgttaca caggcgaggg tatagggtag
     5401 cacaggatgt agctccaatt aacgaggtgc ttgcagcggg gcttatcaag ctatcggggt
     5461 ggaatggcga ttccgtgttt attgacccca tgtgtggttc aggcaccatt ctgattgaag
     5521 cagcactaat tgcctatggt attgctccgg gtattttccg ccaacacttt gggtttgaga
     5581 actggctcga ttttgatgag caactactgc aatccattta caacgatgat agtagggaac
     5641 gtgattttga gtacctgata ctaggccgcg atatatcaaa gcaagctatt gcctcagcca
     5701 tggaaaatat caaggagtcg gggttgaata aaaagattga tttgggggtg gcctcaattt
     5761 tcgattacaa ccctcccagg gtggataagg gtattgtaat aaccaacccg ccttacggcg
     5821 aaaggctaaa gaaggagcag attgaaaatt tctataagca actctccgat gtttttaaga
     5881 ataagtatgc agggtacgat gtttggatta taagctcaaa ccaggaagcg ctcagaagtt
     5941 ttggacttcg tccctcaaaa aaaatcaac
//
LOCUS       DAOSAL010000110         5807 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_8760_length_5807_cov_49.058936, whole genome shotgun sequence.
ACCESSION   DAOSAL010000110 DAOSAL010000000
VERSION     DAOSAL010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 5807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5807
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8760_length_5807_cov_49.058936"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      285..375
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            467..2920
                     /locus_tag="PK049_10820"
     CDS             467..2920
                     /locus_tag="PK049_10820"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOK62093.1"
                     /translation="MKKIAFVLYALLFAGFTMQANPTENLGFTIYGQITDTYGTPLVG
                     ATVKLENTFLGTYSGYNGYYRLTQVRQGSYTLVASYLGYETVQVEVKVNGDMELNLEL
                     KQSSQYLDAVVVSSTRASSRMPIAQTTLNRQELKQANTGGDTPYLLEMLPSVVVSSES
                     GIGIGNTAFRIRGTDPTRINVTINGVPLNDAESQGVYWVDLPDFAASVDNVQVQRGVG
                     TSTNGAGAFGATVNFQTTTLTTEPYAAVDLLGGSYNTWKTSARIATGLMKNGFSMEGR
                     FSQLHSDGYIERANSDHRSMFVTAAWNGERSLVRANIIHGEEHTGITWEGTPDYMMST
                     NRRYNPAGEYVDDQGVTRYYDDQKDNYWQTHYHLMGTHSITNNLTANITLHLTDGRGY
                     YEEYKPDRKLSKYGLPNFSIRDTIIKRTDFIQQKWMDNIFYGGIATMLYRSGIFEYTF
                     GGGWNRYDGDHFGKVLWSKMNIGLPNKYEWYRNNGEKTDWNIFSKAIWQVSDAISVFA
                     DIQYRGISYKLKGIDSDVIALNQEHSWNFLNPKVGATYSFNSGHEAYISYAIAHREPA
                     RSDLKDAQKGSTDFTPKPERLNDWEIGYRMRLQNLSVDFNYYYMLYKDQLVLTGELTD
                     VGYARMANVPNSYRTGIELTLSAKPASWFKIDGNTTLSKNIIKDYTNFANLTDNPTDW
                     NDLQSPVAEPLGNTTISFSPSLVAFTRITILPVENFQFSWVSKYVSRQYIDNTENRNR
                     SLDPYWVNNFITEYSYKTKSNKSIFAQISVNNFFNIKYSANAWVYRTLFQSGDPEYVS
                     IGYYPQAERHIMVRVGIEF"
     gene            3099..3692
                     /gene="pnuC"
                     /locus_tag="PK049_10825"
     CDS             3099..3692
                     /gene="pnuC"
                     /locus_tag="PK049_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HOK62094.1"
                     /translation="MNWLQNNYIELIGAITGLIYLYLEIKQRVWLWPLGIITSAFYIY
                     IFYESKFYADMGLQFYYLFISIYGWYHWLFGGAVKAKNSLPVTNTPKSNYLSLLAASV
                     AFFVIIRHILVHYTDSPVPTGDAFTTALSITATWMLARKYIEHWWLWVIVNSVSLALY
                     LYKGLYPTSVLFVFYTTMSVVGYYQWKQERSKTIAQP"
     gene            3728..4342
                     /locus_tag="PK049_10830"
     CDS             3728..4342
                     /locus_tag="PK049_10830"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HOK62095.1"
                     /translation="MKAVILANGEFPSHPKPLNELLNAPLLVCCDGAIDRLENLAITP
                     NALVGDLDSVKEHLKRKYESILYYDPDQNTNDLTKAVKWCLNHRIKNIVIVGATGKRE
                     DHTLGNIGLLCNYARMGVNVTMLTDTGMLLPLLASTRIDSYAGQQVSIFSPNNQTVIY
                     TKNLKYPIVNQTLPELWMGTLNESLGDWFELTFSPGPVIIYKKY"
     gene            4470..5546
                     /locus_tag="PK049_10835"
     CDS             4470..5546
                     /locus_tag="PK049_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HOK62096.1"
                     /translation="MKHLNSVITGTGSYVPTVRVSNREFARHTFYNKDETAIETLVDE
                     VISKFKDITGIEERRWVTDDLCASHIGAIAAEQAITAAGIDRETIDQIIVAHNFGDVM
                     HGTIQTDILPSLASRIKHHLGIKNPACIPYDILFGCPGWVQGIIQADSYIKSGLAKRC
                     LVIGAETLSRVIDKHDRDCMIFADGAGATIVEALEEDEKRGVISTAMMSHTVNEAFYL
                     YLGKSYHPEADPTIRYIKMEGRKIYEYSLVNVPVAMKVAMDKSGIDPKELKKVMIHQA
                     NEKMDEAIVQRFIRQCKIDKTTTELMPMMIKELGNSSVATVPTLYDLILRGELPEHRI
                     GRGDVIMFASVGAGMNINAIVYKQ"
BASE COUNT         1709 a         1349 c         1260 g         1489 t
ORIGIN      
        1 acgaacaacg agcaacttac acgaataacc tcatttgtca tcctgagcgt agcgaaggat
       61 ctctaatcga cccattgaga tgtttcgcta gcgctcaaca tgacaaaaac cagtattacc
      121 atgaagtttt tccggaaaaa taggattcaa caaaacaaag agaagctggc tttctgacat
      181 cttgattcat acaatggagc aatataaggc aagtccaatc cttgcagttt actatctttt
      241 caccgctcgc ggttcacttc acgctttttt ttactatctt tgcagctgac ttttgggggt
      301 gcccttaggg ctgagatcat accctttgaa cctgatccgg gtaatgccgg cgaagggaaa
      361 aagagtttgt caacatctca caatttcccc ctttatagtt attcaggatt tgcggcttaa
      421 atgtccgaac ctggtttatg tttaactatt taaagggata aaaaacatga agaaaattgc
      481 atttgtgctc tatgcacttc tgtttgcagg ttttaccatg caagcaaatc ctaccgagaa
      541 tctcggattt accatttacg gacagattac cgacacctac ggcactccgt tagtgggtgc
      601 taccgttaag ctggaaaaca cctttttggg tacctacagc ggttacaatg ggtactatcg
      661 gcttacgcag gtgcggcagg gtagctatac ccttgttgcc tcgtacctgg ggtacgaaac
      721 cgttcaggtt gaggttaagg ttaatggcga tatggagttg aatcttgagc taaagcaatc
      781 gtcgcagtac ctcgatgcag ttgtagtatc gtcaacccga gcatcgagcc gcatgcccat
      841 tgcacaaacc acgctcaacc gccaggagct taagcaggcc aacaccggag gcgacacgcc
      901 ctacctgctg gaaatgttac catcggtggt agtatcatcg gaaagcggca ttggaattgg
      961 caacacagcc ttccgcattc gcggaaccga tccaacccgc attaatgtaa ccattaacgg
     1021 ggttccgctc aacgatgctg aatcgcaggg ggtttactgg gtggatttac ccgattttgc
     1081 agcatcggtc gataatgtac aggtgcagcg cggggttgga acatcaacca acggcgccgg
     1141 tgcctttggc gcaaccgtaa actttcaaac cacaaccctt actactgagc catatgcagc
     1201 cgttgaccta cttggcgggt cgtacaatac atggaaaaca tcggcacgga ttgctaccgg
     1261 cctgatgaag aacgggttca gcatggaagg acggttctcg cagttacact ccgacgggta
     1321 tatcgaacga gccaactcgg atcacaggtc gatgtttgtt actgcagcct ggaatggcga
     1381 gcgcagccta gttcgtgcaa atatcattca tggtgaggag cataccggaa ttacatggga
     1441 gggcacaccc gactacatga tgagcactaa ccgccgctac aatcccgcag gcgagtatgt
     1501 tgatgatcaa ggggtaaccc gctactacga cgatcagaaa gataattact ggcaaaccca
     1561 ctaccacctt atgggtaccc actcaatcac aaacaaccta accgcaaata tcacccttca
     1621 cctaaccgat ggcagaggtt actacgagga gtataaaccc gatcgtaagc tctcaaaata
     1681 cggattgcca aacttttcaa ttcgcgatac catcattaaa cgtaccgact ttattcagca
     1741 gaaatggatg gacaacatct tttacggggg tatagccaca atgctttacc gctcagggat
     1801 atttgagtat acattcggcg gaggctggaa ccgttacgat ggcgaccatt ttggaaaagt
     1861 tctttggagt aaaatgaata tcggcttacc caataaatat gagtggtata ggaacaatgg
     1921 cgaaaaaacc gactggaata tattctcaaa ggctatttgg caggtatccg atgccatttc
     1981 ggtttttgcc gacattcagt acaggggtat tagctataag ctaaagggaa ttgactccga
     2041 tgtaattgca ttgaaccagg aacatagctg gaatttcctg aatcccaagg taggggcaac
     2101 ctatagcttt aactcagggc acgaggctta catctcgtat gcaattgctc accgcgagcc
     2161 agcccgcagc gacttaaagg atgcccaaaa gggctcaacc gattttactc ctaagcccga
     2221 aaggctgaac gactgggaaa ttggctaccg catgcgctta caaaaccttt cggttgattt
     2281 caactactac tacatgcttt acaaggatca gcttgtactt actggcgagc tcaccgatgt
     2341 tggttatgcc cgaatggcta atgttcccaa cagttaccgt accggtattg agttgaccct
     2401 ttccgctaaa ccagccagct ggtttaaaat tgacggaaac acaaccctga gcaagaacat
     2461 catcaaggac tatacaaact ttgccaacct taccgataat ccaaccgatt ggaacgacct
     2521 gcaatcgcct gtagctgaac ccctaggcaa cacaacaata tcgttctcgc cttcgctggt
     2581 agcattcaca cgcattacaa ttttacctgt agagaatttt cagttttcgt gggtaagcaa
     2641 gtatgttagc aggcagtata tcgataacac cgaaaacaga aaccgcagcc ttgatcctta
     2701 ctgggttaac aactttataa cagagtatag ctacaagaca aaatcgaata aatcgatttt
     2761 tgctcaaatt tcggtgaata actttttcaa tatcaagtac tccgccaacg cctgggttta
     2821 ccgcacacta ttccagagcg gcgatcctga gtatgtaagc attggttact acccccaagc
     2881 cgaaagacat attatggtta gggttggcat tgagttttaa ttggaaatat gattactggg
     2941 aaatgcaatc gttaaaagat agggagttga ttttggtatt cagggtaaga aagcattcaa
     3001 aatcctgaac tctaagagca aattccctat ctttaccttt taccctttat cctttatcct
     3061 ttagccttta gcctttatcc tttaacttta tccttaaaat gaactggcta caaaacaact
     3121 acattgagct aattggtgca atcactggtc tcatttacct atacctggag attaagcaaa
     3181 gggtttggct ttggccgctg ggcataataa cctcggcatt ctacatctac attttttacg
     3241 agtcgaagtt ttatgccgat atgggcctgc agttctacta ccttttcata agtatttatg
     3301 gctggtacca ctggttgttt ggcggagccg taaaggctaa aaactcatta cctgttacaa
     3361 acactccaaa aagcaactac ttatctttac ttgcggcaag tgttgctttt tttgtaataa
     3421 tccggcacat acttgttcac tacaccgact caccagttcc cacaggcgac gcgtttacca
     3481 cagcgcttag cattactgca acctggatgc ttgcccggaa atacattgag cattggtggt
     3541 tatgggtgat agtcaattcc gtatcgcttg cgctatacct ttacaaggga ctctacccaa
     3601 catcggtgct gtttgttttt tacaccacca tgtcggttgt agggtattac cagtggaaac
     3661 aggagagatc caaaaccatt gctcaaccat aagcacaaat cttactcatt tcaaaaaaaa
     3721 caatattatg aaagcagtta tactggccaa cggagaattt ccctcgcatc caaaaccact
     3781 caatgagctg ctaaatgccc cattgctggt atgctgcgat ggcgccatcg ataggcttga
     3841 gaatcttgcc ataacaccca acgccttggt aggcgacctt gattccgtaa aggagcatct
     3901 caagcgcaag tacgaaagca tactctacta cgaccccgac caaaacacca acgaccttac
     3961 aaaagcagtt aaatggtgct tgaaccatag aataaaaaac attgtaatag ttggcgcaac
     4021 cggaaagcgt gaggatcaca cgttagggaa tatcggattg ctatgtaact acgcccgaat
     4081 gggagttaat gttaccatgc ttaccgatac tggcatgcta ctacccttac tggcatctac
     4141 caggattgac agctatgcag gccaacaggt gtcaatcttt tcgccaaaca atcaaactgt
     4201 aatatacacc aagaacctaa aatatccgat tgtaaaccaa actttgccgg aactatggat
     4261 gggaacgctg aacgagagtt taggcgactg gtttgagtta accttttcac ccggcccagt
     4321 aataatttac aagaaatact aaatttcaat ttataaccat tctatcattc tatttatctt
     4381 tatattaaca ctttttactc tcaattcatt ttgattttag ttacatttta ttacttttac
     4441 agcgggacta atcaaacgtt tattgtatta tgaaacactt aaattccgta atcaccggaa
     4501 cgggtagcta tgtgccaacc gttcgggtta gtaaccgtga gttcgcccgc catacatttt
     4561 acaacaagga cgaaaccgcc atcgaaacgc tggtggacga ggtaatttca aagttcaagg
     4621 acattaccgg cattgaggag cgacgctggg taaccgacga tttatgtgca tcgcacatag
     4681 gcgccatagc agccgaacag gcaattaccg cagccggaat cgaccgtgaa accattgacc
     4741 aaattattgt ggcccacaac tttggcgatg taatgcatgg aaccattcaa accgacatac
     4801 tgccaagctt ggcatctaga ataaagcacc atttgggtat taaaaaccca gcgtgtatcc
     4861 catacgatat acttttcggc tgcccgggat gggtgcaggg cattattcag gccgattcct
     4921 acattaaatc ggggctggcc aaacgttgcc tggtaattgg agccgaaacc ctatcgaggg
     4981 tgattgacaa gcacgaccgc gattgtatga tatttgccga tggagctggc gccaccattg
     5041 ttgaagcact ggaggaggat gaaaaaagag gcgttatatc aacagcaatg atgtcgcata
     5101 cggttaatga ggcattttac ctttacctgg gcaaatcgta ccacccagag gccgacccta
     5161 ctatacgcta cattaaaatg gaaggccgaa aaatatacga gtactcgctt gttaacgtac
     5221 cagttgccat gaaagtggct atggataaat cggggattga tcccaaggaa cttaaaaagg
     5281 taatgatcca ccaggcaaac gaaaaaatgg acgaagccat tgttcaacgc tttatccgcc
     5341 agtgcaagat tgacaaaacc acaaccgaac tcatgcctat gatgataaag gaattgggca
     5401 acagctcagt ggccacggtt cccacccttt acgacctaat cctgcgaggg gaactacccg
     5461 aacacagaat agggagaggt gatgtcataa tgtttgcatc ggttggcgca ggaatgaata
     5521 ttaacgcaat agtttacaaa cagtaataag tattacagcc gggctatttg ctagaggaat
     5581 gaggcggaat aggtggaatg tgatggagtt agaggaagtt ttaatccgtt agacgtgaac
     5641 cgtgaaccgt taaacgggaa aggaaaatat gttatgccca cagtcattcc gaaggataaa
     5701 ggataaagga taaaggttaa aggttctttt acgaacaacg gttcacgaac aacgatcaac
     5761 taatcccctc atcccctctt ttggcgaatc accttcatgt aacttgc
//
LOCUS       DAOSAL010000111         5721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_8924_length_5721_cov_48.932227, whole genome shotgun sequence.
ACCESSION   DAOSAL010000111 DAOSAL010000000
VERSION     DAOSAL010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 5721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5721
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8924_length_5721_cov_48.932227"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..2912)
                     /locus_tag="PK049_10840"
     CDS             complement(3..2912)
                     /locus_tag="PK049_10840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOK62097.1"
                     /translation="MLRFFRFFSLHKFALALSLVAITTLSVVVWQLAQNIDRQNIQTI
                     SADLRSANNDVIRDIERSFWDIKLADYQNDDDNEIIFGKNINLEESSLFNLDNNKLFG
                     TNVLLLHNNRFYAKALFKTSTHEFEYWYPIARTFNLYNALTGNRLAILTDNENTLLSQ
                     PLTLARAKNLTGDKTALIAAEGVKSNSWVLLVLPLLLLVVATAVIYSLLKRSNCRFSG
                     TNSSGKTVHNNAREVLAQINLKSALNNSNVALRVIDTNFRVIFQNQEYEKLTSVTLSK
                     PNHPLCHESFGSIYCHTDECPVRSILNGAKEVKREETRFLPTGKKILVELHAYPLKDD
                     YGNTVAVAEEIKDITGVHYTEEILRQTENQFTVFIDSLPFGVFIEDGLTHEIVYQNFY
                     LKQLTCNTGFKEFIKGAEIIGVDADNNVTEFQLMDSSGIMRYFNLHQFKFLGVNNSLK
                     IGGLFVDNTRKKEIEHYRDVLSKAIEFTPVSIAILSPYFEFEFINPNFTEITGLTIEN
                     LFSKSISDINLEYNGGKHIQKALDSVRAGKTWQGELQLNGKNRERIWVSASFTPVMFE
                     SKLQHILVILENITRRKEYEKELILAKNKAEESDRIKTTFLNNISHEIRTPLNAIIGF
                     SSVLTDPYLGQFERSGFSDLIYKNSQELLQIIENLLEISQIEAGEIKISKTEFSVNAL
                     MNEIYSTFEEQERGSSRIKLSLRKEIFQDDLVVLSDPSRIKQVLKHLLSNSFKFTTNG
                     FVEFGYRLKDENNLLFYVVDSGIGIEPDKMDLIFNPFRQADSSSTRRHGGLGLGLAIS
                     KHVVEKMGGKIWINSTPGSGTSVFFSIPFIPVKLKFENANTGNGLKEFNWDGKTILIA
                     DDIDANFVFFKAALQKTKANLIWARNGLEALNYVRNGEPVNLILMDLVMPEMDGFEAT
                     RIIKSINQKLPVVGQTAYPETISSNKLAECGFDTVLEKPIRTNQMLMEIDRFLVN"
     gene            complement(3019..>5721)
                     /locus_tag="PK049_10845"
     CDS             complement(3019..>5721)
                     /locus_tag="PK049_10845"
                     /inference="COORDINATES: protein motif:HMM:NF012646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOK62098.1"
                     /translation="AIPDFSAQATIPDSLLNAVSVNFEPISNRTIQPNEKAILTLRVS
                     TSPSLTYDTIPIDIDYSSVNYSKASSRFTSKVPVSLFGTTRMTTGTVKTCGTIFTDTG
                     GENSNYNNSENHTLTFETLSDIQRYSVEFISFNTELGYDYLHIYDGAGITSNAITGSP
                     FNGNTGPAPFFTSGNSITFRFTSDGSTTASGWKAKLECITPQQLPKCIENPKPGNNEL
                     NVEYNRLSWRTTSDALFYDVYIGTNPDSLVLLKRVTEAYVDIPLMPQTNYYWRVMPGN
                     HLGLCDKPCEVWNFTTASVIGQVLMTNSTVEVDSLWFYDSGGPTSNYGNNENYTITFK
                     PRNNGQKVKVQFVEFDVESHSTCSYDYMIVYDGPSTQSPELGKYCGDNMPPAYTSTSA
                     NGELTFKFVSDLNTVGTGWKAKITTTGSITTYPVTFNVKNNGSAIPCAAVSTADGIKF
                     TSSSGSVNFSLPNGTYSYTVRANGYEPVLGSTQIAGSAKTIDVNLTKHDTIQLLVRSA
                     LDLAAIPLAKILVNNQTYYTSNLGMATIYIPAGDYTFNLTADGYYAKDTIITVQAGGG
                     NHNINLLPVSYNVQFTISDINGNKIPNAKVFVDTLLGETNSEGLASFTVSRGLKTVSI
                     QKEGFVSTDFWMELSSDSSVNVFLTPVYGDVHQVMFSITGTGPKGTWPLDNAFVKIYN
                     GTQLYHQGKTHNGVATLYLPNGNYSYEVSLEGYQGTEQTEFTVNSNPIEIPVLLNQLT
                     YSITFDVQSGGSPVANATVKINGYSDQLTDANGLATFNQVGYEKGLQFSVLHPNYYTI
                     SGTLDATKSETISVNLTPTSTDIVDPDKNLSMYPNPATSHIRIEANETIESVSIVSIN
                     GTLMHWEQVNNSRHTINLNLPTGVYVVRFTMSNGRLYYRKLMVH"
BASE COUNT         1490 a         1178 c         1298 g         1755 t
ORIGIN      
        1 tattagttca ccaaaaacct atcaatttcc ataagcattt ggttggtgcg tatgggtttt
       61 tccagaacgg tatcaaagcc acactcggca agcttattgc tcgatatggt ttcagggtag
      121 gcggtttggc ctacaaccgg tagtttttgg tttattgatt ttatgatacg ggttgcctca
      181 aagccatcca tctcgggcat taccaggtcc atcagtatta ggttaacggg ctcgccattg
      241 cgaacgtaat ttagggcctc aaggccgtta cgcgcccaaa tcaggttggc ttttgttttc
      301 tgtagggctg ccttaaagaa tacaaagttg gcatcaatat cgtcggctat cagaatggtt
      361 ttgccgtccc agttaaactc ctttaacccg tttccagtat ttgcgttttc aaacttaagt
      421 ttaaccggga taaagggaat ggagaagaat acgcttgttc ccgagccggg cgtgctgtta
      481 atccatatct tcccgcccat cttttccacc acatgcttgg atatggcaag gcctaaaccc
      541 agcccaccat gcctgcgggt gcttgagcta tcggcctggc ggaagggatt aaaaatcaaa
      601 tccattttgt cgggttcaat gccaatgccg ctatcaacaa cgtagaataa gaggttattc
      661 tcatccttaa gtctgtaacc aaactcaaca aaaccattgg tggtaaactt gaacgagttt
      721 gaaagcaggt gctttaaaac ctgttttatt cgggacggat cgctaagcac aactaggtcg
      781 tcctggaaaa tttccttacg caggctcagc ttaatacgcg agcttcctct ttcctgctcc
      841 tcaaacgtgc tgtatatctc gttcatcagg gcattaacgg agaactcggt cttgcttatc
      901 ttgatttcgc cagcttcaat ctgcgatatt tccagcaggt tctcaatgat ttgaagtagt
      961 tcctgtgagt tcttgtaaat taaatcggaa aagccgctac gctcaaattg acccaggtat
     1021 gggtcagtta aaaccgatga gaatccaata atggcgttaa gcggcgttct gatctcgtgc
     1081 gagatgttgt tcaggaatgt ggttttaatc ctatccgatt cctcagcctt gtttttggca
     1141 agtataagtt ccttttcgta ttccttacgg cgagtaatat tctcaagtat tacaaggata
     1201 tgctgaagtt tcgattcaaa cattaccggg gtaaacgagg cgcttaccca aatccgctcc
     1261 ctattcttgc catttagctg tagctcaccc tgccaggttt taccagccct aacggagtcg
     1321 agtgcctttt gaatatgctt gccgccatta tattccaggt tgatatcgga aatgcttttg
     1381 ctaaaaaggt tctctatggt taagccggta atctcagtaa agttcggatt gatgaactca
     1441 aactcaaagt agggcgatag gatggcaata ctaaccggag taaactcaat tgccttggat
     1501 agcacatcgc ggtaatgctc aatctctttt ttgcgggtat tatcaacaaa cagtccacct
     1561 atctttaaac tgttgtttac gccaaggaat ttgaactgat ggagattgaa ataccgcatt
     1621 attccggagg agtccatgag ctggaactca gtaacattgt tatcggcatc cacgccaatt
     1681 atctcagccc ccttgatgaa ctccttgaaa ccggtattac aggtaagctg cttgaggtaa
     1741 aagttttggt aaacaatttc gtgggtaagt ccatcctcaa tgaatacccc aaagggtaag
     1801 gagtcgataa atacggtaaa ctgattctcg gtttgtcgga gtatttcctc agtatagtga
     1861 actccggtga tatctttaat ctcctcggca accgcaacgg tgttgccata atcatccttt
     1921 aacgggtagg catgcagctc tactagaatt tttttgcctg taggtaggaa tcttgtctcc
     1981 tcgcgtttaa cctctttggc tccatttagg atactcctga cggggcactc gtcagtgtga
     2041 caatatatgc ttccgaacga ttcgtggcat aatggatggt ttggtttact gagcgtaacc
     2101 gaggttagct tttcgtactc ctggttctgg aatatcaccc taaagttggt atcaataacc
     2161 ctaagcgcaa cgtttgagtt gttaagtgcc gattttaggt taatttgcgc tagcacttcg
     2221 cgggcattat tatgtactgt tttaccggag gaattagtac ctgaaaagcg acagttcgac
     2281 ctttttaata gtgagtaaat tacagctgta gccacaacca acagcagcaa tggtaaaaca
     2341 agtaaaaccc agctattgct tttaactcct tcggctgcta ttaaagccgt tttatcgcct
     2401 gttaggtttt ttgctcgggc aagggttaat ggctggctaa ggagggtatt ttcattatca
     2461 gttagaattg ctaaccggtt cccggttaag gcgttgtaaa ggttaaaagt tctagcaatt
     2521 ggataccagt actcaaattc gtgggtacta gttttgaaaa gcgccttagc gtaaaacctg
     2581 ttgttatgga gtaaaagcac attggtgcca aaaagcttgt tattgtccag gttgaaaagg
     2641 gatgattcct ccaggttaat gttcttccca aaaataattt cgttatcatc gtcgttttgg
     2701 taatcggcta acttaatgtc ccagaaacta cgctcaatat ccctgattac atcgttgttt
     2761 gctgaccgaa ggtcggcact gattgtttga atgttctgcc ggtcgatatt ttgggctaat
     2821 tgccaaacca caaccgatag cgtggttatg gcaaccaatg ataatgccag agcaaatttg
     2881 tgtaacgaga aaaacctgaa aaaccttaac acttactaaa cacattgatt tgtgccaaat
     2941 gtaaaaatat ttcggtcata taaaaagaaa tgggcatgtt tttatgccca tacttattaa
     3001 caatgttggt tgatatggtt agtgtaccat tagctttcta tagtataacc taccgttact
     3061 cattgtaaac cttaccacgt aaactcctgt tggcaagttt aggttgatgg tgtgcctgct
     3121 attgttgact tgttcccaat gcattaatgt tccgtttatt gaaactatgg atacggattc
     3181 aattgtctca tttgcctcaa tcctgatgtg gcttgttgcc ggattggggt acattgataa
     3241 gttcttgtcg gggtcaacaa tatcggttga ggttggggtg aggtttacgc ttatggtttc
     3301 tgactttgta gcatctaagg ttccggaaat ggtgtagtag ttggggtgta atacgctaaa
     3361 ctgtaacccc ttttcgtatc ctacttggtt aaaggtagct aagccattgg cgtcggtaag
     3421 ctggtcggaa tagccgttaa tttttacggt tgcattggca acagggctac ccccgctttg
     3481 cacatcaaag gtgatgctat aggttagctg gtttagcaga actggaattt cgatggggtt
     3541 gctgtttact gtgaattcag tctgttcagt gccttgatac ccttccaggc tcacctcgta
     3601 tgagtagttt ccattgggca ggtaaagtgt tgcaacacca ttatgtgttt taccttgatg
     3661 gtaaagctgg gtaccgttat agattttaac aaaggcgtta tccagcggcc atgttccttt
     3721 tggcccggtt cctgttatac taaacataac ctgatgaaca tcgccgtaaa cgggggtcag
     3781 gaaaacattt acagagctat cggaacttag ttccatccaa aaatcggtac taacaaaacc
     3841 ctccttttga atgcttacgg tttttaatcc tctgcttacg gtgaatgagg ctaatccttc
     3901 gctattggtt tcgcctaaaa gggtatcaac aaataccttg gcattaggaa ttttgttccc
     3961 gtttatatcg gagatagtaa actgtacatt gtagctaacc ggcaaaaggt taatattgtg
     4021 gttaccacca cctgcttgaa ccgttatgat tgtatccttt gcatagtaac catcagcggt
     4081 gaggttaaac gtataatccc cagcagggat ataaatggtt gccatcccaa ggttcgatgt
     4141 gtagtaggtt tggttgttaa ccagtatttt ggctagcggt atggcggcaa ggtcaagggc
     4201 agaacgcacc agtagttgaa tggtatcgtg tttggtcaaa ttaacatcaa ttgttttagc
     4261 ggagccggca atttgggtgg aacccaaaac tggttcataa ccattggctc ttactgtgta
     4321 gctgtatgtt ccgttaggta gactgaaatt aaccgaaccc gaactcgatg taaatttaat
     4381 tccatctgca gtacttactg ctgcgcaagg tattgcacta ccattgtttt taacattgaa
     4441 agttactgga taagtggtaa tactacctgt agtagtaatc tttgccttcc atcctgttcc
     4501 aaccgtgttc aaatcggaga caaacttgaa tgtcagctcg ccgtttgcgc tcgtcgaagt
     4561 atatgctggt ggcatattat ccccacagta tttaccaagt tcaggacttt gtgtgctggg
     4621 accatcatac acaatcatat aatcatagct acaggttgag tgcgactcaa catcaaactc
     4681 cacgaattga acctttacct tttgaccgtt atttcttggc ttgaaggtaa ttgtgtaatt
     4741 ctcgttatta ccataatttg aggtaggtcc gccagagtcg tagaaccata ggctgtccac
     4801 ttcaacggta gagttggtca ttaaaacctg tccaattact gaagctgtag taaaattcca
     4861 tacctcgcag ggtttatcac ataaacccaa atggttgccg ggcattacac gccagtagta
     4921 attagtttgc ggcataagcg gaatatcaac atatgcctcg gtaacccttt ttagtagaac
     4981 taaagaatcg gggttggttc ctatgtaaac atcgtagaaa agggcatcgg aggtggtcct
     5041 ccacgataac cgattatatt caacattaag ctcattattc ccgggttttg ggttttcaat
     5101 acacttgggc aattgttgtg gagtaataca ctctagcttt gccttccacc ctgaggccgt
     5161 tgttgagcca tcggatgtga aacggaatgt aatactgttt ccgcttgtaa aaaagggagc
     5221 ggggccggta ttgccattaa acgggctgcc agttatggca tttgaggtta taccagcccc
     5281 atcgtaaatg tggaggtaat cgtatcccaa ttcggtattg aacgatataa actcaacact
     5341 atatcgctga atatcgctta gggtctcaaa ggttagcgta tggttctcgc tgttattgta
     5401 gttggagttc tcgccaccgg tgtcggtaaa aatagtccca caggttttaa cagtaccagt
     5461 ggtcatcctg gtggttccaa aaagggaaac tggtaccttg ctggtaaacc ggctcgaagc
     5521 tttgctatag ttaacggagc tataatctat gtcaatgggg attgtatcgt aggttaagct
     5581 tggtgatgtg cttacccgca gggtaagaat tgctttttcg ttaggctgaa tggttcggtt
     5641 tgaaatgggt tcaaagttga cacttaccgc gttgaggagt gaatcgggaa tggttgcctg
     5701 tgccgagaaa tcgggaatgg c
//
LOCUS       DAOSAL010000112         5704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_8956_length_5704_cov_47.395645, whole genome shotgun sequence.
ACCESSION   DAOSAL010000112 DAOSAL010000000
VERSION     DAOSAL010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 5704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5704
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8956_length_5704_cov_47.395645"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="PK049_10850"
     CDS             complement(<1..293)
                     /locus_tag="PK049_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK62099.1"
                     /translation="MSQTSRKRVLIVTYYWPPSGGVAVQRWLKFVKYLRDFGWEPIVY
                     TPENPESFYDDHSLFKDIPENIEVIKRPIWEPYDIYKWITGRKGKKLGLGFAD"
     gene            413..1369
                     /locus_tag="PK049_10855"
     CDS             413..1369
                     /locus_tag="PK049_10855"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HOK62100.1"
                     /translation="MVLFKVVAWLILVYGIIALVAKKARPSGFDLPFALPAILVGAIL
                     VVILSLIVVVGPQEVAVVTTPSGVRSEPLHTGWHVIAPWNDIHYMDKTVWVYTCANAI
                     GEGEKPNSDAIWAPTKDGIKLGFDVSVSWRIDANEAPWIYQNVTENDGGRSGRYLWLE
                     ENVIRAKLKSALALTVSGYTPIEAYSTKRNDIQQEILNRIKDECKQYRIIIDNVDVRE
                     VYYNEEYEKAINAKKLAEQEALRLIEVTKQKEEQLKQATIDKNIAIEKAKGESEALKI
                     KGQSISSNPKIIELEWINKWNGQLPTYMMGNGQGIMVNLNNK"
     gene            complement(1441..1962)
                     /locus_tag="PK049_10860"
                     /pseudo
     CDS             complement(1441..1962)
                     /locus_tag="PK049_10860"
                     /inference="COORDINATES: protein motif:HMM:NF012696.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
     gene            complement(2071..3612)
                     /locus_tag="PK049_10865"
     CDS             complement(2071..3612)
                     /locus_tag="PK049_10865"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOK62101.1"
                     /translation="MKTLDELYQELERRNAIAEQAGGSDRIAKQHESGKKTARERIVE
                     LLDPGTFNEFDKFVTHRTNDFGMDKNRIPGDGVVTGYGKIDGRLVYVYAYDFTVFGGT
                     LSRANADKIIKLQQLATRMGAPIIGLNDSGGARIQEGVQSLAGYAEIFYNNVRSSGVI
                     PQVTCIMGPCAGGAVYSPALTDFIIMVKDSSYMFVTGPDVIKAVTHEDVTKDELGGAV
                     THNAKSGVAHFIAENDEQALMLVRELMSFLPVNNMEEPPVFPTTDDPLREDESLQTLI
                     PADPNKPYDIKELILTVVDDKNFLEVQPLFAKNIVVGFARMAGHTVGIVANQPAHLAG
                     VLDIDASVKGARFIRFCDSFNIPIVTFVDVPGFLPGVNQESNGIIRHGAKLLYAYSEA
                     TVPKIAVITRKAYGGAYCVMSSKQVGADVNYAYPMAEIAVMGPEGAVNILYRNKIDSE
                     ETRQELIDEYRETFANPYRAAELGYIDEVIYPKQTRAKLIQALEMTRTKSQQNPPRKH
                     GNIVL"
     gene            complement(3645..4154)
                     /locus_tag="PK049_10870"
     CDS             complement(3645..4154)
                     /locus_tag="PK049_10870"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HOK62102.1"
                     /translation="MGLEVRIGDRIAEVKLVRRDENIAVFEVDGVEYEVDIAQVENGV
                     FSIIHNGKSFNIEIIPGDGPKNYYVNTFYNSHEVEIIDAQSRYQRSRKALDDHAADKI
                     ISTPMPGRVVKIPVSEGETVSKGTTVIVISAMKMESEYKSPVDGIVKKIFVAEGDNIN
                     ANQPLVEIE"
     gene            complement(4172..5683)
                     /gene="accC"
                     /locus_tag="PK049_10875"
     CDS             complement(4172..5683)
                     /gene="accC"
                     /locus_tag="PK049_10875"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HOK62103.1"
                     /translation="MIKKVLIANRGEIAVRVIRSCREMGILSVAVYSDADRRALHVRY
                     ADEAYHIGPSPSRESYLNGDKIIEVALKAGADAIHPGYGFLSENADFARKCAEAGITF
                     IGPDPQAINQMGDKITARQTMIAAGVPVVPGTQQKVSDMETVRKIVDEIGLPVIIKAS
                     AGGGGKGMRLVRKPEELISAVKMAQSEALSAFGDDTVYMEKYVESPHHIEFQILADRH
                     GNTVHLFERECSIQRRHQKVVEETPSPFLTPELRDEMGKAAVAAAKAVNYVGAGTIEF
                     LVDANHNFYFLEMNTRLQVEHPITERVTGIDLVKEQIRIASGLPISFKQDEIAQHGHA
                     IECRILAEDPFNNFMPSPGLIRHISEPNGLGVRTDGYAYEGYEIPIYYDPMISKLITW
                     GRDRTEAINRMKRALFEYKITGVKTSIRFLERIMDNHDFRMGNYNTHFIPEHLDELMD
                     IQPRGSKIFEDMAIIAAYFDFITKMEKVAMSDFTVTHSDNWKTYGLKRSLLPY"
BASE COUNT         1458 a         1345 c         1340 g         1561 t
ORIGIN      
        1 tcgtcggcaa agccaagtcc taactttttc ccttttcgcc ctgttatcca tttgtaaatg
       61 tcgtagggtt cccaaattgg ccgtttaatt acctcaatgt tctcagggat atctttaaac
      121 agggaatggt cgtcgtaaaa ggattcaggg ttctcagggg tgtaaacaat tggctcccag
      181 ccaaaatcgc gaaggtactt tacaaacttt agccaacgtt gcacggcaac accacccgat
      241 ggaggccagt agtatgttac tatcagtacc cttttacgcg atgtttgact catacaaaaa
      301 tttttgttaa agatagatag attagccaat tccaacaaat gggggcatca cttgattgtc
      361 aattcaaatg tttttcatac ctttcctaat ctaacttaaa atgcataagt gtatggtact
      421 ctttaaagtt gttgcgtggt taatccttgt ttatggcata attgcattgg ttgccaaaaa
      481 ggcaaggcca tcggggtttg accttccctt tgctttgcca gccattttgg ttggtgctat
      541 acttgtagtg atactttcgc tgattgtggt tgttgggccg caggaggttg ctgtggttac
      601 cacaccaagt ggtgtccgct ctgaacctct gcacaccgga tggcatgtaa tagccccgtg
      661 gaacgatatt cactacatgg ataaaaccgt ttgggtttac acctgtgcca atgccattgg
      721 cgagggcgaa aaacctaact ccgatgctat ttgggcacct accaaggacg gtattaagct
      781 tggttttgat gtatccgttt cgtggcgcat tgatgccaat gaagctcctt ggatatacca
      841 aaacgttacg gaaaatgacg gtggccgatc gggacgttac ctctggcttg aggagaatgt
      901 aatccgcgca aagctgaaat cggcattagc tctaaccgtt tcgggttata ctcccattga
      961 ggcatacagc actaagcgga acgacatcca acaggaaatc cttaaccgca ttaaggatga
     1021 atgcaagcag tatcgaataa taattgataa cgtggatgtg cgcgaggttt actataacga
     1081 ggagtacgaa aaggctatta atgctaaaaa attagctgag caggaagcct taaggttaat
     1141 tgaggtaacc aagcaaaagg aagagcagct caagcaggct actatcgata agaatattgc
     1201 cattgagaaa gctaagggag agtcggaggc tcttaaaatc aagggtcagt ccattagtag
     1261 caatcctaag atcattgaac ttgaatggat caataagtgg aatggtcagc ttcccacata
     1321 catgatgggg aatggccagg gaataatggt taaccttaac aacaagtagt aacccctact
     1381 gaactagcga agccggtaat cccggcttcg tttgctatga tttatctgca attaaaatat
     1441 ctattcaagg cgatatatcg acataatttc cttaagaata gcatcggtat cgacctttaa
     1501 atctttcagc aggcccgttt ccatatcgaa aacccagcca tgcactttga gtgttttgga
     1561 acggatagct ttttgaactt caggggcctt gataacgttt acacattgca ccctcacgtt
     1621 cagttctacc agacgtcttt gcctatcggt aatagagtta cttgtttcaa tttcgtgtcg
     1681 atggtggcga taaatatcac gtatattccg tagccaggga ttgagtatat tcatatcttt
     1741 aggctgcagt gcagcctcaa tgcctccaca gtggtagtga cctcaaacta caatatgttc
     1801 aacccttagt accgttacag catagtttat tattgccatt gaggcaaggt cgccagcggg
     1861 aacgaggttg gcaatattgc ggtaaacaaa aacatcgccc ggatttgccc ccataagctc
     1921 ttcagctgta acccggctat cggaacagcc aatgtaaagc attttgggtt gctggtttag
     1981 cgaaaggttt agaaagaaac tggggttaac gcgcttgcgc gcttcagccc agttcctgtt
     2041 attctcaaaa acctttttga tgtccatgag ctacaaaaca atattaccat gcttgcgggg
     2101 tgggttctgc tggctcttgg tacgggtcat ctccaatgcc tgaataagct tggcacgagt
     2161 ttgttttggg tagataacct catcgatata gcccagttca gcagcacggt aggggtttgc
     2221 aaatgtctcg cggtactcat caattagttc ctgacgggtt tcctcgctgt caattttatt
     2281 cctgtacagt atgtttactg ctccctcggg gcccataaca gcaatctcag ccatgggata
     2341 ggcgtagtta acatcggcgc caacctgctt gctggacatt acgcagtatg caccaccgta
     2401 tgccttgcgg gtgattaccg caattttagg aactgttgct tccgagtaag cgtatagcag
     2461 ctttgctcca tggcgaataa taccattcga ttcctgatta acacccggta gaaatcccgg
     2521 aacatcaaca aaggttacaa tgggaatgtt gaacgagtcg cagaagcgaa taaagcgggc
     2581 gcccttaacc gatgcgtcga tatcgagcac gccggcaaga tgggcaggct ggttggcaac
     2641 tatgccaacg gtgtgtcccg ccatacgtgc aaagccaaca acaatatttt tggcaaaaag
     2701 cggctgaacc tccagaaagt ttttatcgtc aaccacggtt aggattaact ccttgatatc
     2761 gtagggttta tttgggtcgg ctgggattag ggtttgtaga ctttcgtcct cgcggagcgg
     2821 gtcatcggtt gttgggaaaa cgggtggctc ctccatgttg ttaacgggta gaaaactcat
     2881 tagctcccta acaagcataa gtgcctgttc gtcgttttcg gcaatgaagt gcgctacgcc
     2941 gcttttggca ttatgggtaa cagctccgcc cagctcatcc ttggtaacat cctcgtgtgt
     3001 aacggctttt atcacatcgg gtccggtaac aaacatgtag ctggaatcct taaccataat
     3061 aataaaatcg gttagggctg gtgagtacac tgcaccgcca gcgcaaggtc ccattataca
     3121 ggttacctgt gggataaccc cggaactacg aacgttgtta tagaatatct cggcatagcc
     3181 agccaaactc tgaacacctt cctgaatacg tgcaccaccc gagtcgttca gcccaataat
     3241 tggtgcgccc atacgggtgg ccaactgctg aagcttgata atcttatcgg cgttagccct
     3301 actaagcgta ccgccaaaaa cagtaaaatc gtatgcataa acgtaaacca accgtccgtc
     3361 aatcttgcca taaccggtta ccacgccgtc gccgggtatt cggttcttat ccatgccaaa
     3421 atcgttggta cggtgggtca caaacttgtc aaattcattg aaagtaccgg ggtcgagcag
     3481 ttcaacaata cgttcccttg ctgttttttt cccactttcg tgttgtttag caatacgatc
     3541 ggatccgcca gcctgctcgg ctatggcatt gcgtcgctct aactcctgat atagctcatc
     3601 aagtgttttc atggaaaaaa ataataaagg tttaaggata gaatttactc aatctcaaca
     3661 agcggttggt tagcattgat attatcgccc tcagccacga atattttctt tacaatccca
     3721 tcaacggggc ttttgtactc gctttccatt ttcatggccg atataacaat caccgttgtt
     3781 ccttttgaaa cggtttcgcc ttcagatacc ggaatcttaa ccaccctacc gggcatgggt
     3841 gttgagatta tcttatcggc agcatggtcg tcgagagcct ttcggcttcg ttggtatcgg
     3901 ctttgggcgt caataatttc cacctcgtgg gagttgtaaa aggtattcac gtagtaattt
     3961 tttggcccat cgccaggaat aatctcaatg ttaaacgatt tgccattatg gattatggaa
     4021 aaaacaccgt tctcaacctg ggcaatgtcc acctcgtact caacgccatc aacctcaaaa
     4081 acggcaatat tttcatcgcg gcgcaccagc ttaacctcgg ctatgcgatc gccaattcta
     4141 acttcaagtc ccattgttca agcatgtttg tttagtaagg aagcaagctg cgctttaacc
     4201 cgtatgtttt ccagttatcg gagtgggtta cggtaaaatc gctcatggcc accttttcca
     4261 tcttggtgat aaaatcgaaa taggctgcaa ttattgccat atcctcaaag attttactgc
     4321 cacgaggctg aatatccatc agttcatcaa ggtgttcggg tatgaagtgg gtgttgtagt
     4381 tacccatcct aaagtcgtgg ttatccatga tacgctcaag gaagcggatg gatgttttca
     4441 ccccggtgat tttgtactca aacagagccc gtttcattcg gttgatggcc tcggtacggt
     4501 cgcgccccca agtgataagc ttgctaatca tggggtcgta gtaaatagga atttcgtagc
     4561 cctcgtaagc atagccatcg gttctaacac caagcccatt gggctcgctg atatgcctga
     4621 taaggccggg cgatggcata aagttgttga acggatcctc ggcaagtatg cggcattcaa
     4681 tggcatgccc atgctgggca atctcatcct gtttaaacga tattggcagg cctgaggcaa
     4741 tgcggatttg ttccttaacc agatcgatgc cggtaacacg ctcagtaatg ggatgttcca
     4801 cctgcaggcg ggtgttcatt tcaaggaagt agaagttgtg gttggcatca accagaaact
     4861 cgatggttcc tgctccaacg tagttaacag ccttagcagc tgccacggcc gctttaccca
     4921 tctcatccct aagctcaggg gtaagaaaag gcgatggggt ttcctcaacc accttttgat
     4981 gccttcgctg tatggagcat tcgcgctcaa acagatgaac agtattgccg tgcctgtcgg
     5041 caagtatttg gaactcgata tggtgaggcg attcaacata cttctccatg taaacggtat
     5101 cgtcgccaaa ggccgatagg gcttcggact gagccatttt cacagccgat atcagttcct
     5161 cgggcttgcg aaccagccgc atacctttcc cgccaccacc ggccgaagcc ttgatgatta
     5221 ccggcaggcc aatctcgtca acaatcttac gaacggtttc catgtccgaa actttttgct
     5281 gggtcccggg tacaacaggt acgccggcag caatcatggt ttgacgggcg gttatcttgt
     5341 cgcccatttg gtttatggct tgggggtcgg gtccaataaa ggttattccg gcctcggcgc
     5401 atttacgggc aaaatcggcg ttttccgaaa ggaaaccgta gcccgggtga atggcatcgg
     5461 cacctgcttt cagggctacc tcaataatct tatcgccatt caggtagctt tcccttgagg
     5521 gcgatggccc gatgtggtat gcctcatcgg catagcgaac atggagtgca cgcctgtcgg
     5581 cgtcggagta aaccgctacg gagagtatcc ccatctcgcg gcacgaacgg ataacccgga
     5641 ctgcaatctc gccccggtta gctattaata cttttttaat catagttgtc ccagatatga
     5701 atcc
//
LOCUS       DAOSAL010000113         5674 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_9001_length_5674_cov_44.328528, whole genome shotgun sequence.
ACCESSION   DAOSAL010000113 DAOSAL010000000
VERSION     DAOSAL010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 5674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5674
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9001_length_5674_cov_44.328528"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..1093)
                     /locus_tag="PK049_10880"
     CDS             complement(5..1093)
                     /locus_tag="PK049_10880"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOK62104.1"
                     /translation="MKNITIKVDTHRNEEVICFLCDKDIQLIEILKTKLNARWSVTKK
                     YWYLPTSQFNLNKTFEAFKSIAWLDIKNLPHTSNIDESQTDIQQEKERFEKWLKFRRY
                     SPNTIKIYTDALVVFLSYFDHKSVSELTNDDVVLFVNQYILKRKYSFSYQNQIVNALK
                     LYFREIRKSKIEVEGLQRPRREHKLPNVLSMDEVKAILQAPTNVKHRTMLSLIYACGL
                     RRSELLNLKIGDVDSKRHMLIIRNSKGYKDRQVPISDKTIEMLREYYKMYRPKVWLFE
                     GQKEGEQYSEQSLQSVLKQAVSKSNIRKPVTLHWLRHSYATHLLESGTDLRFIQELLG
                     HKSSKTTEIYTHVTEKSLQKIISPFDHL"
     gene            complement(2395..2961)
                     /gene="frr"
                     /locus_tag="PK049_10885"
     CDS             complement(2395..2961)
                     /gene="frr"
                     /locus_tag="PK049_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOK62105.1"
                     /translation="MNLDDAKKVIDIAESKMQKAIEHLDNELRVIRAGRANPAMLSGV
                     MVDYYGTMTPLTQVASVGSPDARTIVVQPWEKKMIDPIEKAIMAANLGFNPQNNGESI
                     RIMVPPLTEERRKDLVKQVKHEGENARVSIRNARRDSLEEIKKMQKNGLPEDVAKDAE
                     TKIQKITDNYNKKVDDVLHKKEQEILTV"
     gene            complement(2998..3708)
                     /gene="pyrH"
                     /locus_tag="PK049_10890"
     CDS             complement(2998..3708)
                     /gene="pyrH"
                     /locus_tag="PK049_10890"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOK62106.1"
                     /translation="MIKYKRILLKLSGEALMGDDKYGINTQVLTSYAKQIKEVADLGV
                     EIGIVIGGGNIFRGLSGVSKGFDRVKGDQMGMLATVINSLALQSAIEGAGGKAKVFTA
                     TKMEPVGELYTKAKVIEGFKQGFVAIIAGGTGNPFFTTDTASALRGVEIEAEVLLKGT
                     RVDGVYTADPEKDKTAVKFDELTFTDAYEKKLKVMDLTAFTMCSENNLPILVFDMNTP
                     GNLMKVVTGERIGTLVKN"
     gene            complement(3748..5088)
                     /locus_tag="PK049_10895"
     CDS             complement(3748..5088)
                     /locus_tag="PK049_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK62107.1"
                     /translation="MKKLMIFLGIALTIGVILAPLKSKAWSEHPLLAYPVLSSIPDLK
                     NRNPVEAQSLKAFLIKEEKGLAEFLMRYEEWAKTNLPNYAPLPQGLEFKHTGNQSDIV
                     YRFLTALRLNPNIKLRLYLHLLPNQDINGKTVIPPSELTTLTDVSSLSHTNYVVLNEG
                     ELVAPLQVLCTANDEPDYGFDIGLFEDNGTSYGKTYGFGVQPFGDPKLEYGSQAPFHM
                     GFYHEAKLVYAFGPFLRKTYPEYRISLFKALAEYAFATGNDYWGWRFMGWGMHYLGDL
                     SMPYHTAPLPGMSAARMIWINLKAIIGFPKSKNDAVQLVSNKHSVLEQYQWIAMREAY
                     SNYDTNPLIEALRAEPPITPYSNDFARNVVSQISVDRSRKVDKALKEFCPPLLVKDPS
                     FEASKSKELDNLMDQINNYSGANGIEQLNALLTEMLRTYSMSLRSYYNAIVSKA"
BASE COUNT         1417 a         1225 c         1203 g         1829 t
ORIGIN      
        1 ttttttacaa atgatcaaat ggacttataa ttttttgtaa acttttttca gttacatggg
       61 tgtatatttc ggtagtcttt gaacttttat gacctaaaag ttcctggata aagcgtaaat
      121 cagtccctga ttcaagcaag tgtgtggcat agctgtgtcg aagccaatgc aaggttactg
      181 gttttctaat gttacttttc gaaacagcct gttttaatac gctttgcaga ctttgttctg
      241 aatattgctc gccttctttc tgtccttcaa acaaccatac tttgggtcta tacattttat
      301 agtattctct aagcatctca attgttttgt ctgatattgg gacttgcctg tctttgtacc
      361 cttttgaatt tctaattatc agcatatggc gtttagagtc cacatcgccg atttttaggt
      421 tcaataattc gctgcgacga agtccgcatg cgtatatcag gcttagcatg gtgcgatgct
      481 ttacgttggt tggggcctgg agtatggctt taacctcatc catgcttaaa acatttggca
      541 gcttgtgctc ccgacggggg cgttgcaacc cctcaacctc tattttgctt ttgcgtattt
      601 cccggaagta aagttttagt gcattaacta tttggttttg atacgaaaag ctatacttgc
      661 gctttagtat atattggttt acaaataata ccacatcgtc gttggtcagt tcagaaaccg
      721 atttatggtc aaaatagctg agaaaaacaa ccaaagcatc tgtatagatt ttgattgtat
      781 tggggctata acgtctaaac tttagccatt tttcaaacct ttctttttct tgctgaatat
      841 ctgtttgaga ttcatctatg tttgatgtat gcggtaggtt ctttatatcc aaccatgcta
      901 tacttttaaa tgcttcaaaa gttttgttta aattgaactg cgaagtgggc agataccaat
      961 atttctttgt aacactccaa cgagcattga gcttagtttt aagtatctct attagttgta
     1021 tgtccttatc gcataaaaag catataactt cttcgttgcg gtgagtatca acttttatag
     1081 taatgttttt catgttcaat gggtgaatat tgggacctta cctacacttt gctgaatagg
     1141 ctattgtttt aagctcttct ttttcaattt gtcacataat tcttttagta aagttagaaa
     1201 atttatttat tcgagtcaca tcaattaatt atatcaattg acgtatctgt gtttatgtct
     1261 gtgatgatag ggttagtagt tagatatcaa tgttgtttag ttaaggttat ttgtcatgtt
     1321 gcgcgcaagc gaaacatcac gctgtcgtgc tgagtaccga tttatcggga tagacttcgc
     1381 cgaagcatct cattgctttt ttctcttcaa acctttgaag agatccctcg actttgctcg
     1441 ggatgacaaa aaccctatct tcctttctgc tcacttgtca tgctgagcaa gccgaagcat
     1501 ctctttacat taaacccttt aattcttgtt gttttttgga aaccgctctt ttccatgctc
     1561 ttcagttgcg atgcaacgct tccaccttgc tctttggcat atacattacg tgcatcgcaa
     1621 cacacgaggg agaagccatt gggttacaaa tatgtcgccc ctacggggct ggaaaggttg
     1681 ttcattgctc gttgctcgtt gttcgtgtaa gctaagtcat acactctctg aaatttgatg
     1741 ttgatcggtt cataagacta ttgtcatgct gagcccaatc gaagcatttc tttccccttt
     1801 tattctttta cgagatccct tgacaacgct cgggattaca acacccttag cttcctcact
     1861 caccttgtca tgctgagtcc agccgaagca tttctttccc cttttattct tttacgagat
     1921 ccctcggcta cgctcgggat gacaacaccc ttagcttcct cactcacctt gtcatgctga
     1981 gtccagccga agcatctcat tgaacctaca actcttcatt aatgcgattc ctcgactcgc
     2041 tcaagatgac aggagtagtt gctggttgtt cgtgtatcgt tgttcgtgaa tgccattctg
     2101 taacaacatg ttacactgtg taactctgtg ataacaattt agttgattgt tgttggttga
     2161 tggcttaaga aaccaccttc aatcatgaat ctgaaatctg agatctgaaa ttttaaattt
     2221 ttatccttta tccttaaaca tcacccccca ctgtacactg cacactgaac acggtacact
     2281 aaatatggga ttcctcgaca aagattttcc ctgtatatcg aggctaagga tgtgatggat
     2341 aaaaaaaggt gttaccggtt aagtaacacc cttgcattac ggaacgttca aatcctaaac
     2401 ggtaagtatt tcctgctcct ttttgtggag cacatcatct actttcttat tgtagttgtc
     2461 ggtaatcttt tggattttgg tttcagcatc cttagcgacg tcctcgggca ggccattttt
     2521 ctgcatcttc tttatttcct caagggaatc gcggcgggcg ttgcgaatgc tcacgcgggc
     2581 attctcaccc tcgtgcttaa cctgcttaac caaatccttt cggcgctcct cggttagggg
     2641 aggtaccatt atgcgaatcg attcgccgtt gttttggggg ttaaagccaa ggttggcggc
     2701 catgatggct ttctcaatgg ggtcgatcat ctttttttcc cagggttgca ccacaatggt
     2761 gcgggcatcg gggctgccca cgcttgccac ctgggtgagc ggggtcattg ttccgtagta
     2821 gtcaaccata acaccggaaa gcatggccgg gtttgccctt ccggctctga taaccctaag
     2881 ctcattatcc aaatgctcaa tggccttttg catcttgctc tcggcaatat ctataacttt
     2941 ttttgcatcg tcaaggttca tggctttatg attttattgt ttcggacact aaaagtatta
     3001 attttttacc aatgtaccaa ttctttcgcc tgtaacaact ttcattaggt tgcccggggt
     3061 gttcatatcg aatacaagga taggaaggtt attctcgcta cacatggtaa aggcggtaag
     3121 atccatcacc tttaactttt tctcgtaagc gtcggtaaag gttagctcat cgaactttac
     3181 ggctgttttg tccttttcgg gatcggcagt gtaaacacca tcaacacggg tgcctttcag
     3241 gagaacctct gcttcaatct caacaccgcg taatgctgag gctgtatcgg ttgtgaagaa
     3301 aggattcccg gttccgcctg caataatcgc cacgaagccc tgcttgaacc cttcaataac
     3361 ctttgctttg gtgtagagct cgccaacggg ctccatcttg gtggcggtaa aaaccttagc
     3421 ctttccgcct gctccctcaa tggccgattg tagcgccaaa ctattaatta ccgtggccag
     3481 cattcccatc tggtcgccct ttacccggtc gaatcctttg gaaacgccac tcaaaccccg
     3541 aaaaatattt cccccaccaa taacaatccc aatttcaacg ccaaggtcag caacctcctt
     3601 tatctgtttg gcatagctgg taagcacctg ggtgttaata ccgtacttat cgtcgcccat
     3661 aagcgcttcg ccactaagct taagcagtat ccgtttatac tttatcattg tttacatttt
     3721 tagggcgtaa aaatagcaaa ttatcggtta tgctttgcta acaattgcgt tgtagtagct
     3781 tcgtaacgac atgctgtagg tacggagcat ctcagtaagc aatgcgttaa gctgttcaat
     3841 cccatttgct cctgagtaat tgttgatttg atccataagg ttgtcaagct cttttgattt
     3901 actggcctca aacgatgggt cttttaccag aagtgggggg cagaattctt ttagggcttt
     3961 gtcaacctta cggcttcggt cgaccgatat ttggctcacc acgttacggg caaagtcgtt
     4021 ggaatagggt gtaatggggg gttcagctct aagcgcttca attagtgggt tggtatcata
     4081 gttgctatat gcctcacgca tggctatcca ttggtactgc tcaagtacgg agtgtttatt
     4141 tgataccagc tgaacggcat cgtttttgct ttttgggaag ccaataatag ccttgaggtt
     4201 aatccaaatc attcgagcgg cgctcattcc gggtaggggg gctgtgtggt acggcatcga
     4261 taggtcgcca aggtagtgca ttccccagcc catgaatcgc caaccccagt agtcgttgcc
     4321 ggttgcaaat gcgtactcag ccagtgcctt gaaaaggctg atgcggtatt cggggtaggt
     4381 tttcctgagg aatggaccaa aggcgtaaac cagctttgcc tcgtggtaaa accccatgtg
     4441 aaaaggagcc tggctgccgt actcgagctt aggatcgcca aagggttgaa cgccgaatcc
     4501 ataggttttt ccgtagctgg tgccattatc ttcaaacaaa cctatgtcaa atccgtagtc
     4561 gggctcgtcg ttggcggtgc acagcacttg gagcggagca accaattcgc cttcattaag
     4621 caccacgtag ttggtgtggc ttagcgaact aacatcggtc agagtggtta attccgaggg
     4681 cggaataacg gttttcccat taatatcctg gtttggaagt aaatgtaagt acaatctcag
     4741 cttgatatta gggtttaacc tgagggctgt gagaaaacgg taaacaatat cgctttggtt
     4801 accagtgtgc ttaaactcaa ggccctgtgg taatggagca tagttgggta agttggtttt
     4861 agcccactcc tcgtatcgca tcagaaattc agctaacccc ttttcttctt ttataagaaa
     4921 agctttaagg ctttgcgcct caacagggtt ccggtttttg aggtcgggaa tgctgctcag
     4981 aaccgggtat gccagtagcg ggtgttccga ccatgctttt gatttaagag gagctaaaat
     5041 aacacctata gtcaacgcta tacctaaaaa aatcatcagc tttttcatcc gtacaaaata
     5101 tttatattat taattactaa aacgttaatg tcttgctttt aacaaacttt atcgagcgga
     5161 tggaacaccc atagtttgac ttcgtcgagc tcgcaaaaaa cgctcggttg ttcatctgtg
     5221 tttgtgcaag ctgccttaca tgggtgtttc atggcgtcaa agtttttaca atagtatgaa
     5281 aaaattttgg taagtatatc gttttgaatc atttatcaaa ggtacaatga attaacaaat
     5341 tcaaccgtgc tagtaaaaac gggaaggcgg aaactttttt gaggaatgag tcggaatgag
     5401 tcggaatgaa tcggaatgaa tcggaatatg atagggttag agggagtttt gtttgttaaa
     5461 cgtgaaccgt gaaccgttaa acgagcggct gtgacccgta ctgaaaaatg tctacactaa
     5521 tttatgcgta tggacaaaag caattacgtg acaaaacgct acagcgaggg cttcaaactg
     5581 aaaattttaa cccaagttcc atggaaatcg ttcaattttg cttggctaac cacaaatctc
     5641 attattactt taatactgcg tcattagtca ttat
//
LOCUS       DAOSAL010000114         5597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_9145_length_5597_cov_46.810718, whole genome shotgun sequence.
ACCESSION   DAOSAL010000114 DAOSAL010000000
VERSION     DAOSAL010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 5597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5597
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9145_length_5597_cov_46.810718"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(194..1201)
                     /gene="gap"
                     /locus_tag="PK049_10900"
     CDS             complement(194..1201)
                     /gene="gap"
                     /locus_tag="PK049_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOK62108.1"
                     /translation="MTKIKVAINGFGRIGRNVFKIALERPELEVVGINDLTDTKTLAH
                     LLKYDSTQGRYEGKVEFDAENLIVNGKKYRVTAERSPLNIKWSETPDVVIESTGIFTK
                     RESEKGGYGDHIKNGAKKVILSVPAKDEIDRMIVLGVNDEALQPTDQCVSNASCTTNC
                     LAPVVKVLNDAFGVERGFMNTIHSYTNDQRILDAPHSDLRRARSAAVSQIPTTTGAAK
                     AVGKVIPELKGKLDGLAVRVPTPTGSLVDFVAVLKKEVTKEEVNAAMKKAAEGPMKGI
                     LEYTEDPIVSVDIIHNPHSSIFDAQSTMVNGNMVKVLSWYDNEWGYSNRVVDLIHKLF
                     K"
     gene            complement(1354..1932)
                     /locus_tag="PK049_10905"
     CDS             complement(1354..1932)
                     /locus_tag="PK049_10905"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HOK62109.1"
                     /translation="MQKVTLAILAGGKSSRLGGIDKALLTVDGIPLISRIYNTFRTEV
                     WEVIVISNSSKTYPIDAPIFPDIILNCGPLGGIHSALTNASSSLVFIVSVDLPFASKD
                     IFQQLLCIYQKNRAEIVVPKHGDLMEPLFGLYNKTLLSRVNSILADRQGHPISELISQ
                     SNTKYLELPDDFQTRMWFYNINTHEDLKALEL"
     gene            complement(1939..2562)
                     /locus_tag="PK049_10910"
     CDS             complement(1939..2562)
                     /locus_tag="PK049_10910"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005633198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOK62110.1"
                     /translation="MPECLYIPMHPQNPNPRDIDRVVAVLQRGGVVIYPTDTVYGIGC
                     DITNVKAVERVIKLKGLKPKDAHFSFICKDLSNIADFAKVSNPTFKLMKKNLPGPFTF
                     VLPGLHRVLDYFLSKRKTVGIRIPNHQIPLELVDRLGNPILTTSLKDSDEVVEYTTDP
                     ELIYENYKHLVDVVIDGGVGGNIPSTIVDCSGDEPIILREGLGELIF"
     gene            complement(2562..3218)
                     /gene="fsa"
                     /locus_tag="PK049_10915"
     CDS             complement(2562..3218)
                     /gene="fsa"
                     /locus_tag="PK049_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HOK62111.1"
                     /translation="MKFFIDTANLDQIREANDLGVLDGVTTNPSLMAKENIRGAENIK
                     KHYVDICNIVKGDVSAEVIATDYDGMIREGKELAALHPQIVVKVPCTKDGIKAIKYFT
                     DNGIRTNCTLVFSVGQALLAAKAGATYVSPFIGRLDDVSTDGVELIRKIVDVYNYYEF
                     GTQVLAASIRHTMHIIQCLEAGADVATCPLSAILGLLNHPLTDIGLQKFLDDYKKVNG
                     "
     assembly_gap    3618..3717
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3718..4383
                     /locus_tag="PK049_10920"
     CDS             <3718..4383
                     /locus_tag="PK049_10920"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOK62112.1"
                     /translation="VQVLDKCDNITQAFESIKHHNPDIVFLDIEMPGGSGFDLLHMLR
                     NTPYNPTIVFITAFNQFAIKAIKHAAFDYLLKPIDIDDINDTLARYREKQRNGIKKVD
                     KSKAELLLEHLEGFSKLKFNMRNGVLFVDPDDIIWCEASGSYTVLHLHSRKDEVVSVS
                     LKDVEQQLHQYPFFRVSRSAIINLKYLTKIDRRNKVCIVQKNDLVFKITGSPQQLKAL
                     ENR"
     gene            4524..4949
                     /locus_tag="PK049_10925"
     CDS             4524..4949
                     /locus_tag="PK049_10925"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOK62113.1"
                     /translation="MGSFEQFNWKGKQILIVEDDKPSSILLTALLSRTGVKLYFATNG
                     QQAVDIVKTNNSIDLILMDIKLEGMNGIQASTIIHEHNPNIPIIAQTACVMTGDRENC
                     MAAGCVAYVSKPIDAYELLQIIDKYINGVKIKSAAETIP"
     gene            5167..>5597
                     /locus_tag="PK049_10930"
     CDS             5167..>5597
                     /locus_tag="PK049_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005778254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOK62114.1"
                     /translation="MEFKLISDFKPAGDQPEAINQLTEGLIRGDRYQTLLGVTGSGKT
                     FTIANVIANLNRPVLVLSHNKTLAAQLYGEFKAFFPENAVEYFVSYYDYYQPEAYLPV
                     TDTYIEKDLSINDEIEKLRLSATSSLLSGRRDVIVVSSVSCL"
BASE COUNT         1637 a         1207 c         1050 g         1603 t
ORIGIN      
        1 cgaatcatct cttttctctt ctacacccac ttgccgttgc ccgatatcct tcgctacaac
       61 attatgacaa aaagaggatc tgttttttta tctgaacgat attgaatgat aaacatatgt
      121 cagaacttta cctatcaact atcaataaaa aaaggctgct tgaaggcagc cttttctaat
      181 cctacaacgc ttattattta aatagtttat gaattaggtc aaccacgcgg ttagagtaac
      241 cccactcgtt atcgtaccat gaaagaactt taaccatgtt gccattaacc atggtgcttt
      301 gagcatcgaa aattgaagag tgagggttgt gaataatgtc aaccgataca attggatcct
      361 cagtatactc aagaataccc ttcatagggc cttcggcagc ctttttcata gcagcgttaa
      421 cctcttcctt ggtcacctcc ttttttagca cagctacaaa atcgacaaga gaaccggttg
      481 gggttggaac gcgaactgca agaccatcga gtttaccctt cagctcagga ataacctttc
      541 caactgcttt tgcagcaccg gtagtagtgg gaatctgtga aacggcggca gaacgagcac
      601 gacgcaaatc gctgtgaggg gcatcaagaa tacgttgatc gttggtgtag gagtgaatgg
      661 tgttcatgaa accacgctca acgccaaaag catcgttcaa aacctttaca acgggggcca
      721 agcagttggt tgtacagctg gcatttgata cgcactgatc agttggctgt aaggcttcat
      781 cgtttacacc aagtacaatc atcctatcaa tctcatcctt tgcgggtacg ctaagaataa
      841 ccttttttgc accattcttg atgtggtcgc catagccacc cttctcgctt tcgcgcttgg
      901 taaaaatacc tgtcgattcg attaccacat cgggagtctc gctccacttg atgttaaggg
      961 gtgagcgctc agctgttacc ctgtactttt taccgtttac aattaggttt tcggcatcaa
     1021 actctacctt accctcatag cgaccctggg tggaatcgta ctttaacagg tgggcgagag
     1081 ttttagtatc ggttaaatcg ttgataccta caacttcaag ttcgggacgc tcaagcgcaa
     1141 tcttaaaaac gttgcggcca attcgtccaa atccattgat tgcaacttta atttttgtca
     1201 tagctagttg aagttagtta ttatgaattg tgagtttaaa attcagccta acaaaggtac
     1261 ttaaaattaa acaaattgtt aatgtaaaat ttgaatattc aacaattttt aacgcaaacg
     1321 gttgagaacc aaaggttgtt agcaggttac aaattatagc tctaaggcct ttaggtcctc
     1381 gtgggtatta atattataaa accacatccg ggtctgaaaa tcatcaggta actctaggta
     1441 ctttgtgttt gactgactta taagttctga tatagggtgt ccttgcctgt cggcaagaat
     1501 actgtttact ctcgataaaa gtgttttgtt atacaagcca aacaagggtt ccattaaatc
     1561 gccatgtttg gggacaacaa tctcagccct gtttttctgg taaatgcata gcaactgctg
     1621 gaatatgtcc ttactggcaa atggtaggtc aacagagaca ataaatacca aactgcttga
     1681 agcattggtt aaagcgctat ggataccgcc aagaggtcca caattcagta taatatcagg
     1741 aaaaatgggc gcatcgatgg gataggtctt gctgctgttt gaaattacaa ttacttccca
     1801 aacctcagtt ctaaaagtat tgtaaattct acttatcaag ggaatcccat caactgttaa
     1861 aagagcttta tcgattcccc ctaatcggct cgatttgccg ccagctaaaa ttgcgagtgt
     1921 aaccttttgc atgatgacct agaaaatcag ttcgccaagc ccttcgcgca gtattatagg
     1981 ctcatcgcct gagcaatcta caatggttga aggtatattg ccaccaactc ccccatctat
     2041 tacaacatct actaaatgct tgtaattttc ataaatgagc tcaggatcgg ttgtgtactc
     2101 tacaacctca tcgctatcct ttaacgatgt tgttaggata gggttgccta atctatcaac
     2161 caactctaat ggaatttggt gattgggtat tcttatacca acagttttcc gtttactcaa
     2221 aaagtaatcg agaaccctgt gcaaacccgg taaaacaaat gtaaaaggac cgggcaaatt
     2281 ctttttcatc aacttaaatg tggggttgct aaccttggca aagtcggcaa tattgcttag
     2341 atctttacag ataaaagaga aatgggcatc cttgggcttt aaccccttga gcttgatgac
     2401 acgttcaacc gcctttacat ttgtaatatc gcaacctata ccgtaaaccg tatcggtagg
     2461 ataaataaca actccacccc tttgcagaac cgcaaccacc cggtcaatgt cccgtgggtt
     2521 ggggttctgg gggtgcattg gtatgtatag gcattcaggc attaaccatt caccttttta
     2581 taatcgtcta aaaacttctg taatccgata tcggttaatg ggtggttaag taaaccaaga
     2641 attgcactta atgggcaggt agcaacatca gcaccagcct cgaggcactg gataatatgc
     2701 atggtatgac gaattgaggc ggctaaaacc tgggtaccaa actcgtagta gttgtaaaca
     2761 tcaacaattt tacggattaa ctctactcca tcggtcgata catcatcgag tctaccaata
     2821 aatggtgaaa catatgttgc tcctgctttt gctgcaagca aggcctgtcc aaccgaaaac
     2881 acaagtgtac agtttgtgcg tattccgtta tcggtgaaat acttaatggc ctttatgcca
     2941 tccttggtac acggaacctt tactacaatt tgaggatgca aggcggctaa ctctttaccc
     3001 tcgcggatca tcccatcata atcggtagca attacctcag cgctaacatc gccctttaca
     3061 atgttgcaaa tgtctacgta atgctttttg atgttctcag caccacgaat attttccttt
     3121 gccatcaacg atgggttggt agtaacgcca tcaagaacgc caagatcatt tgcctctcgg
     3181 atttggtcaa ggtttgctgt gtcaataaaa aatttcatac ctttttgttt tagcttgatt
     3241 tactgccgca aaactaatca atttagtcgg atgtggcaat ttaaaatggg attttagtag
     3301 attcagaact taaagaaaag ccaagggtgg tgcgtgagcc gtgaaccgtg aacggcgaaa
     3361 cggaatcgaa caactaacca cgctcttgtc aatccgagca tcctatccca ctttagcggg
     3421 gtttaagttt caaggtttaa tagcttgtcc gctatcgact accaactagg taattatcaa
     3481 tgtgttacac agattcaaca cggtgtttca cggtgtatgt cagcctaatc tgtgtcatca
     3541 gcgttccatt tcacatctca atcaacgaac agctaatcca aataaatctg ctctaatact
     3601 tgcttatatt ccaaaaannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngtt
     3721 caggtgttgg ataaatgcga taacattacc caagcctttg aatcgattaa gcatcacaac
     3781 cccgatattg tttttttgga tattgaaatg cccggtggtt caggttttga cctcttacat
     3841 atgcttagga atacccccta caaccctacc attgtcttta ttacagcctt taaccaattt
     3901 gccattaaag ccataaaaca tgccgcattc gattatctgc ttaaacccat tgatatcgac
     3961 gatataaacg acacccttgc ccggtaccgc gagaaacagc gtaatggtat aaaaaaagtt
     4021 gacaaatcta aagcagaact gctgctcgaa catcttgaag ggttttctaa gcttaagttc
     4081 aacatgcgca acggggttct ttttgttgac cctgacgata tcatctggtg cgaggcaagc
     4141 ggtagctata ctgttcttca cttacattcc cgaaaggatg aggttgtctc ggtttcgctt
     4201 aaggatgtag agcagcaact tcatcagtat ccctttttcc gtgtaagccg ttcagcaata
     4261 atcaatctga agtatctcac caaaattgac cgtaggaata aggtatgtat tgttcaaaaa
     4321 aacgacttgg ttttcaaaat cacaggctcc cctcaacaac tcaaggcact ggaaaatcga
     4381 taattacttc aaatattttt acttcaaccc cgaaataagc attttcgtaa accaaaacat
     4441 gcgtttcata cattaaccgc aattctctat tccacatctt atttcaaagg tttaacttga
     4501 gcaaggttta caaatgcaac aaaatgggct cgtttgaaca attcaactgg aaagggaaac
     4561 aaattttaat agtagaagac gacaaaccta gttccattct acttactgcc cttttaagcc
     4621 gaacaggtgt taagctttac tttgccacta atgggcaaca ggctgttgat attgtcaaaa
     4681 ccaataactc aattgatcta atcctaatgg atataaagct tgaaggcatg aatggcattc
     4741 aggcatcaac aatcatacac gaacacaatc ccaatattcc aattattgct caaaccgcct
     4801 gcgtaatgac cggcgatagg gagaattgca tggcggctgg ctgtgtggca tatgtttcaa
     4861 aaccaattga tgcctacgag ctactacaaa taattgataa gtatataaac ggggtaaaaa
     4921 tcaagagtgc tgcagaaact attccttaag ctaatacaca ttcaattatc acttctctaa
     4981 ttttgtttaa tttcttttta aaccgggcta aaagattata agcccagaac ctaattcatg
     5041 cttaacccat aaccctttag agtaaaaaca gggtaagcta cccgattttg agctatgaag
     5101 ttattaactg tttatattac actgaacact gcaaactaat tgctaatatt actacctttg
     5161 cagattatgg aattcaaact aatatcggat tttaagccgg caggcgatca gcctgaagca
     5221 ataaaccagc taacggaagg attgatacgt ggcgataggt accaaaccct gcttggggta
     5281 accggctcgg gtaaaacatt taccattgct aacgttatcg ccaaccttaa tagacctgtt
     5341 ttggtgctta gccacaataa aaccctggca gctcaacttt acggagagtt taaagccttt
     5401 ttccccgaaa atgccgttga gtactttgtt tcatactacg attactacca accggaagcc
     5461 taccttcccg taaccgatac ttacattgag aaagaccttt ccattaacga cgaaattgaa
     5521 aagttaaggc ttagtgccac ctcctcattg ctttcaggaa gacgcgatgt tattgttgtt
     5581 tcctcagtat cgtgcct
//
LOCUS       DAOSAL010000115         5488 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_9372_length_5488_cov_56.247193, whole genome shotgun sequence.
ACCESSION   DAOSAL010000115 DAOSAL010000000
VERSION     DAOSAL010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 5488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5488
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9372_length_5488_cov_56.247193"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="PK049_10935"
     CDS             <1..419
                     /locus_tag="PK049_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK62115.1"
                     /translation="FALIWLNPKTRIIPESWAVAVEWGLTNTEYHILGQKYGSYKALS
                     YNFKEGKQFWYRGNDEFYTPLFIDLIDDQNQRINNNGSILFPNDKVKGYSLSILESIL
                     FGVRDLELLKAMLKINKPFGVANEDIDELINFYKNI"
     gene            424..864
                     /locus_tag="PK049_10940"
     CDS             424..864
                     /locus_tag="PK049_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK62116.1"
                     /translation="MKPIKIIYKLLISIGVIMFFSYASCDKWVCGDWWFINLTEHKIS
                     VDVYCFGSISTELSFSLMPNDSLKQITCDIGSSPPPFVSSTCRIDSILLVFDDTLVLN
                     TLNYWEFNPENIEKWENYNRKSNKKYNFTESHYQKALEVNGYSR"
     gene            997..1419
                     /locus_tag="PK049_10945"
     CDS             997..1419
                     /locus_tag="PK049_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK62117.1"
                     /translation="MKAYKLYLFAVIVSFLNSCGDKDVCEDYRFENATEHRVYIRSCC
                     GEATDSNTSLSIGPKSSATLTSCALGYSDFLYNRLSGCVVDRVLIIFDDTLELNTRDF
                     GEFDLLNWDNYQRKNKEYIYEINQLHYQKALEVNGYLR"
     gene            1825..2247
                     /locus_tag="PK049_10950"
     CDS             1825..2247
                     /locus_tag="PK049_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOK62118.1"
                     /translation="MLRRRSTLPSLVDEFFGDDFISRFFDDSESVTIPSVNIKEGKDD
                     YTIEVAAPGFDKKDFKVDLNNNVLEISSEKEVKEENKDEKVMRREFRYSSFKRSFTLP
                     DTADTDKIKASYKDGILSIIIPKKEEAKVKPVRQISIS"
     gene            complement(2296..2754)
                     /gene="pyrI"
                     /locus_tag="PK049_10955"
     CDS             complement(2296..2754)
                     /gene="pyrI"
                     /locus_tag="PK049_10955"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HOK62119.1"
                     /translation="MKDKKHLSVSAIENGTVIDHIPAQNLFKVITILGLDRIPNQITF
                     GTNLESKLLGKKAIVKIADKYFERDEINRIALVAPHAKLNIIKNYEVTEKMLVEVPND
                     ISGIVKCVNPKCITNVENVTPKFKVVDKNSVSLKCHYCEKITDQEHMEYL"
     gene            complement(2767..3678)
                     /gene="pyrB"
                     /locus_tag="PK049_10960"
     CDS             complement(2767..3678)
                     /gene="pyrB"
                     /locus_tag="PK049_10960"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HOK62120.1"
                     /translation="MKNRSLVAINDLSRDEIFRVLDLAEEFEKNPIQRIMEGKVVATL
                     FFEPSTRTRLSFESAVNKLGGRVIGFSEASTSSVSKGETLKDTILTVANYSDLIVMRH
                     PVEGSARYASEVSPVPIVNAGDGANQHPTQTLLDLYSIRKTQGKLDNLKIVMVGDLKY
                     GRTVHSLLMAMSQFNASFTFISPAELRMPLEYKMYLDELGLKYEEHTDLAENIKDADI
                     VYMTRVQRERFSDPMEYEKVKNAYILKNYMLEGTKPNMRVLHPLPRVNEINTDVDESP
                     KAYYFTQALNGVYTRMAIMALILGLKK"
     gene            complement(3742..4359)
                     /locus_tag="PK049_10965"
     CDS             complement(3742..4359)
                     /locus_tag="PK049_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOK62121.1"
                     /translation="MVEVADTYKTIDSPAEGIYKEKGSKFLAKAYPVRTEEQVKAIVE
                     QLKEEYYDARHHCYAYILGPKGDKWRANDDGEPSGTAGKPIHGQLLSFNLTNVLVVVI
                     RYFGGTKLGVSGLINAYKTATHDALTNAKIITRTVDAIYSVTFGYTMMNEVMRLAKDL
                     NLQLLEQQFDNTCIIRVRIRRSLEGEFLSRCSKIEGLIADFEFED"
     gene            complement(4360..5241)
                     /locus_tag="PK049_10970"
     CDS             complement(4360..5241)
                     /locus_tag="PK049_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK62122.1"
                     /translation="MFYLLLCILSSTVVLITFKLLNRFSVNNFSAIIINYLAASVAGF
                     FLAKGELSGFYQLGILPILLMVAMGVLFITMFHFLGKSTQTAGVAITSVAAKMSVIIP
                     ILVSVLIDPNDKLNAVRLAGILLALIAVLLLVIPRKTEKFKPVKTYLPLIIFLGIGMV
                     DASVKVAQQYFVTNELNPLFNAVVFAMAGITGLLLLPFNTHAAKDFHKFETWLLGTLL
                     GLANFGSMFFMVAALNHVGSKGTPLEGSVLFGINNLGVVLAGTLVGLLLFGERPSKTN
                     LSGIALSVLAIVILMQS"
BASE COUNT         1605 a         1050 c         1160 g         1673 t
ORIGIN      
        1 catttgccct tatatggctt aatccaaaaa ccagaataat cccggagagt tgggcggttg
       61 ctgtagaatg gggacttacc aatacggaat atcacatact ggggcaaaag tatgggagtt
      121 ataaggctct gagttataat tttaaagaag gcaaacaatt ttggtatagg ggtaatgatg
      181 aattttatac accgctattt attgatttga tagatgacca aaatcaacgc attaataata
      241 atggctctat tttatttccg aatgataaag ttaagggtta ttccctttcg attcttgaga
      301 gtattctttt tggtgtaaga gatcttgaat tacttaaagc catgctaaaa ataaacaagc
      361 cttttggtgt tgctaatgag gatattgatg aattgattaa tttctataaa aatatttaaa
      421 cctatgaagc cgattaaaat aatttacaaa ttattaatat caataggggt aattatgttt
      481 ttttcatatg ccagttgtga taagtgggtt tgtggcgatt ggtggtttat aaatttgaca
      541 gaacataaaa tatctgttga tgtatattgt tttggcagta tcagtactga attatcattc
      601 tcattaatgc ctaatgattc cctaaaacaa ataacatgtg acatagggtc ttcaccaccg
      661 ccattcgttt cgagtacttg cagaatagat tcaatactgt tagtttttga tgatactcta
      721 gtattaaata ccttaaatta ttgggaattc aatccagaga acattgagaa atgggaaaat
      781 tacaacagga aaagtaataa aaagtataac tttaccgagt cgcactacca aaaagcgctt
      841 gaggtgaacg ggtactcaag gtaaaaacgg tctccctgcc ggtaacggag ggggacttta
      901 acacaatttg aatagttcag tgccaaaatt attactaaaa gctctaaggg tcttactaat
      961 aaaaaaaatg catacttttt ttaaatttta aaagcgatga aagcatataa attataccta
     1021 tttgctgtaa ttgtatcctt tctaaattct tgtggagata aggatgtatg tgaagattac
     1081 aggtttgaaa atgccacgga acatagggtg tatatccggt cctgctgcgg ggaagcaacc
     1141 gatagcaata catcccttag cattggccct aagagtagcg ctacattaac cagttgtgct
     1201 ttaggatatt ctgatttttt atataataga ttaagcggct gtgtagtgga tagagtgctc
     1261 attatttttg atgatactct agaacttaat acacgggatt ttggggagtt tgacttgcta
     1321 aactgggata attatcagcg caagaataag gaatatattt acgaaataaa tcaactgcac
     1381 taccaaaaag cgcttgaggt taacgggtac ttaaggtaaa acggtctccc tgccggtaaa
     1441 cggctaagag tgaaatatct caggcaagtg tgaaataaaa aagagcaaaa aagatgcttc
     1501 agcgttcagc atgacagtgt gatgtttatg tggagtttat cccgtaatgt caggaatcaa
     1561 cagggcaact atacctaact aaacgacatt gaaattgcgt ttacaaacaa aatcaatgaa
     1621 atttaaatca agccatttaa tcacacctaa ttcatattga ttgagtttga gtcaatttta
     1681 tacttttctg tgacaaattg acctttgttt tactagtttt atgaaaaatt gacacaaata
     1741 aagggttggc atacggtttg aaaaatgcta ggtgaaagca ttgatgtcta accaaaataa
     1801 tgggagggta aaactatgat gccaatgtta cgtcgcagaa gcactctacc aagtctggta
     1861 gatgagttct ttggggatga ttttatcagc cgtttctttg acgacagcga gagtgtaaca
     1921 atcccttcgg taaacatcaa ggagggaaag gacgattaca ccattgaggt ggctgctccc
     1981 gggttcgaca agaaggactt taaggttgac ttgaacaaca acgtgctgga gatttccagc
     2041 gagaaagagg ttaaggagga gaataaggac gaaaaggtaa tgcgtcgtga gttccgttac
     2101 tcctcattca aaaggtcgtt caccttaccc gatacagccg acaccgataa gattaaagct
     2161 tcgtacaagg acggcatctt aagcattatc atcccaaaga aggaagaagc taaggttaaa
     2221 cccgtacgtc agatctcgat atcgtaacgg gaagatgtta aaaacagcga agccccggaa
     2281 acggggcttt actgtttata ggtactccat gtgttcctgg tcggtaattt tttcgcagta
     2341 gtggcacttt aacgacacgc tattcttgtc aaccaccttg aatttagggg taacattttc
     2401 aacgttggtg atacactttg ggtttacgca ttttacaatg ccacttatat cgttgggtac
     2461 ttcaaccagc attttctccg ttacctcata gtttttaatg atgttgagct tggcatgagg
     2521 agcaacaagg gctattcggt taatctcatc gcgctcaaag tacttatcgg caatcttaac
     2581 aatagccttt ttgccgagta gcttactctc caggtttgtt ccaaaggtta tttggtttgg
     2641 tatcctatcg agtccgagga tggtaataac cttaaacagg ttttgcgccg gtatatggtc
     2701 aattactgta ccattctcaa tggcgctaac actcaggtgt ttcttgtctt tcatatcgat
     2761 attcttttac ttttttagac ctaaaataag agccatgatc gccatacggg tgtaaacgcc
     2821 gtttagtgcc tgggtaaagt agtatgcctt tggcgattca tctacatcag tatttatctc
     2881 gtttaccctg ggtagcggat gtagcactcg catgtttggc tttgtacctt caagcatata
     2941 gtttttcagg atgtaggcgt ttttaacctt ttcgtattcc atggggtcgg agaagcgttc
     3001 gcgctgaacc cgggtcatgt aaacaatatc ggcatcctta atgttttcgg ccaaatcggt
     3061 atgctcctcg tactttaagc ctaactcatc gagatacatt ttatactcta gtggcattcg
     3121 gagttcggcg ggcgaaataa atgtaaatga ggcgttaaac tggctcatgg ccatcaacag
     3181 ggagtgaacg gttcggccat acttaaggtc gcctaccatt acaatcttca ggttatcgag
     3241 tttaccctgc gttttgcgaa tggagtaaag gtcgagaagg gtttgggttg gatgctggtt
     3301 ggcaccatcg cctgcattaa caatgggaac aggcgatacc tcgcttgcat agcgtgcgct
     3361 accctccacg gggtgtcgca tcactatcag gtcggaatag ttggctacgg tgagaatagt
     3421 atccttaagg gtttcgcctt tggacacact actggttgat gcctccgaaa agccaattac
     3481 ccgtccacct agcttgttta cagcgctttc aaagctcaag cgggttcggg tcgacggttc
     3541 aaagaaaagt gttgccacca ctttcccttc catgattcgc tgaatagggt ttttctcaaa
     3601 ctcctcggca aggtcgagca ctctgaatat ctcatccctg ctcagatcgt tgattgctac
     3661 caaacttctg tttttcatag tttttatttt gtgtttaagt atatggttca aaaaaaagcc
     3721 ccgataaacg gggctagtac atcaatcctc aaattcaaaa tcggcaataa gtccttcaat
     3781 cttggagcat ctggaaagga actcgccttc cagtgagcgc ctaatcctta ccctgataat
     3841 gcaggtatta tcaaattgct gctctagtag ttgaaggttt aaatccttgg ctaatcgcat
     3901 cacctcattc atcatggtgt aaccaaaggt tacactgtaa atggcatcaa cagttcgggt
     3961 gataattttt gcattggtta aggcatcgtg ggttgccgtt ttatatgcat ttatcaagcc
     4021 cgatactcct agctttgttc ccccaaagta acgtattacc accactaaaa cgttggttag
     4081 gttaaagctg agcagttgcc cgtgaatggg cttgccggca gtacccgatg gttcgccgtc
     4141 gtcgttagcc ctccatttgt cgccctttgg ccctaaaata tatgcgtagc agtgatgccg
     4201 ggcatcatag tattcttcct taagttgctc aactattgct tttacttgct cctcggttct
     4261 tacagggtag gctttagcga ggaacttact gcctttttcc ttgtaaattc cctcggcagg
     4321 cgaatcgatg gttttgtatg tgtcagctac ttcaaccatt tagctttgca taagtattac
     4381 aattgccaat acagaaaggg ctatcccgga aagatttgtt ttgcttggcc tttcaccaaa
     4441 tagtagcagc ccaaccagcg ttcctgccag tactaccccc aaattgttta tgccgaaaag
     4501 aaccgatccc tccaagggag ttcctttgct tccaacatgg ttcagtgcag caaccataaa
     4561 aaacatcgat ccaaaatttg ctaatccaag caatgttcct agtagccatg tttcgaattt
     4621 atgaaagtct ttggctgcat gggtattaaa aggtagcagt agtaagcctg taatgccagc
     4681 catggcaaat acaaccgcat tgaacaaggg atttagttcg ttggttacaa agtattgctg
     4741 tgcaacctta accgacgcat ctaccattcc aatacccaaa aagattatta gtggaagata
     4801 tgttttaacc ggtttaaatt tttccgtttt tcttggtatt acaagtaaga gaactgcaat
     4861 taaggcaagc aaaatgcctg ctaatcgtac tgcatttaac ttatcgtttg ggtcaatcag
     4921 taccgatacc agtatgggga ttattaccga catcttagct gctaccgacg ttatggcaac
     4981 accggctgtt tgcgtactct tacccaggaa atggaacatg gttataaaga gtacacccat
     5041 agccaccatc agtagtatgg gtagtatgcc tagttgataa aaccccgata gttcaccttt
     5101 ggccaaaaaa aaaccagcta cgctggctgc caaatagtta atgatgattg cactaaagtt
     5161 gttgactgaa aagcggttga gtaacttaaa ggtgattaaa actactgtag atgataatat
     5221 gcagagcagt aagtagaaca tccttttagg tttgtgcaaa gttatagcat ctttacttac
     5281 cccacaaatt tttttagagg taagtattaa catacaatta acatgcaatg tagttttaaa
     5341 tgagaacaac cgtaatttgt catcctgagc gcagcgaagg atcttttgga tagttttcag
     5401 tgtacagggg tttttagtgt ttagtgtttg gtgtttagtg tttcgtgaaa tgcttttttt
     5461 aactatcaac tatcaaccat caactgtc
//
LOCUS       DAOSAL010000116         5470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_9403_length_5470_cov_52.421791, whole genome shotgun sequence.
ACCESSION   DAOSAL010000116 DAOSAL010000000
VERSION     DAOSAL010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 5470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5470
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9403_length_5470_cov_52.421791"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..1004)
                     /locus_tag="PK049_10975"
     CDS             complement(117..1004)
                     /locus_tag="PK049_10975"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOK62123.1"
                     /translation="MKTDRISLSPLVWGHWRLTEWNLSQRELLYLTQQCIDLGITSID
                     SADIYGNYSCEKLFGNALALEPSVRNHIQIITKCGIRLISGKFPEVTVKHYDYSYNHI
                     VSAVETSLKNFRTDHIDLLLLHRPSPLLNPGEVALAFNHLYSTGKVLHFGVSNFLPHQ
                     VEMLQSFTPIHLVANQIEISPLQVEHLQNGNLDYLMQKHIIPMAWSPLGGGRLVSPKN
                     KTDKRVSKVIHKIANETSLLPEQVAISWLTTHPSGIIPVIGSGKFERIKNAYNALNTK
                     LSIQQWFEVYSASTGEDVP"
     gene            complement(1015..>1878)
                     /locus_tag="PK049_10980"
     CDS             complement(1015..>1878)
                     /locus_tag="PK049_10980"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOK62124.1"
                     /translation="ISKRVTRGVEFAQEISKGNLQAAFNDNSDDEIGTLSKSLTNMAA
                     RLRDTFRGIGQVSESIMDTGQTMNTNSEALTSTVGELTLATNEVSHSIQRMVGSIGQS
                     TKSVQDQLNISTKAVETIKQGSQTSAKALDAMKMVANKIQVVNDIAFQTNILALNAAV
                     EAARAGEHGSGFAVVAGEVRKLAERSKQAAQDIVNLANSSLATVEEVRNVMEKLAHEV
                     NRIADHAKQLSFEFDDQQQETNRIGDSMQKLSAVSKLTSDIANDLRTYSEHLLKLAAN
                     LKDASSFYKID"
     assembly_gap    1879..1978
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1979..2760)
                     /locus_tag="PK049_10985"
     CDS             complement(<1979..2760)
                     /locus_tag="PK049_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK62125.1"
                     /translation="MKPLNLRRLQSKMLILLVGISAIVYLIVFVFIIKSIQHDNLKDA
                     HSYVNTLVKETARSFEDQLNQELSITRTLAEAFRAFNNTSQKDWIDLQNRMVSDVLKE
                     HHNIKSLWVNWDLSKIDKTGKSKGRLRCTFYWENDVLKYRQDTVTFDNLSSKSLWVTK
                     NIKGNTILEPYYWKYSNSDKVKIPMTSLVSPISYGNQLVGTVGCDITLTEIKDRVDKL
                     KLFENSYAFLLSNGSVYVSHPDTTVLGKTMKEINAEEDSIHH"
     gene            2908..3369
                     /locus_tag="PK049_10990"
     CDS             2908..3369
                     /locus_tag="PK049_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK62126.1"
                     /translation="MKNRLNYFPIYIGITYTITMLSCGRALPTNDDLKSEILDVESQF
                     CDYVMRHGIAEGFAFFADESATILCGRDSLIKWKNAIYHYYSDTQYKGAKVEWKPEFV
                     QVSKCGDIAYTYTTHGRFQKQMPIHWLCKEYFIRFGSAIPMEPGSTFRINL"
     gene            3381..4208
                     /locus_tag="PK049_10995"
     CDS             3381..4208
                     /locus_tag="PK049_10995"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HOK62127.1"
                     /translation="MEPEKQFWQSSSGKKLFAWHIKPAATPKAIILIVHGHGEHSLRY
                     VPWAKRFAYHGFIVQSWDHIGHGQSEGRRGHIRYFEQLLFEIDLAITKITHDYPKLPI
                     ILYGHSMGGNIALNYAINRPTKLDLLVVTSPWLKLAVPPSPVMDVLSKFMNLVAPFIQ
                     IKSTVKPDQISQIPEEVEKYATDPLNHGLITPRLYTSITSSTKYIFGNAQKISIPTLL
                     MHGSGDTITSHKATDELSHIIPKATYIEWSGCYHELHHEQERDEVFNSILNWINEQL"
     gene            4195..5196
                     /locus_tag="PK049_11000"
     CDS             4195..5196
                     /locus_tag="PK049_11000"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HOK62128.1"
                     /translation="MSSSKIDFRTEVTVNNFPFRISYQTPVLFCGSCFAENIGGIMHS
                     YKMPVQVNPFGVVYNPFSVARVLRNILQNKEYTSNDLHSRNGLWFSFDHYTRFSSESK
                     DECLLKINEACSSAHEFIKKANLIGVTFGTARVYRLVSTGKVVANCHKIPAKEFTHTL
                     LSVDEIVSEWTKLIDEVLAVIPNIRFLFTVSPIRHWKDGPIGNQLSKSTLILAVHRLV
                     EIYSDKAFYFPGYEIMMDDLRDYRFYDSDMLHPSQLAIDYIWQKFSDALIEHQAMQVT
                     EKVHRIQQALSHRPVNPKSQEFKRFVDIILNEVSSIQKQYPFLNFNEEVNKLKSYIV"
     gene            5193..>5470
                     /locus_tag="PK049_11005"
     CDS             5193..>5470
                     /locus_tag="PK049_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK62129.1"
                     /translation="MILFKSLRELLYVFSVTAYLFFAATNVFSQDSTWVWREKGSSSL
                     KFFGLIAYQEGRGEQGFGIPLGIGFQKHYLNGRWRVNTSIQAASYRTFA"
BASE COUNT         1521 a         1069 c         1145 g         1635 t
ORIGIN      
        1 cccgccaatg cgggacaggt tctgaaatct gaaatctgaa atttgctcaa tgcaaaacag
       61 gtttttgtat ctgaaaaaat cattgctaaa aggcaatgag taaaatttcc gaaaaatcag
      121 ggtacatcct ctccagtaga tgccgagtac acttcaaacc attgttgaat tgaaagtttc
      181 gtgttcagag cgttataggc attttttatt cgctcaaatt ttccgcttcc aattactgga
      241 ataattccag atgggtgagt ggtaagccag ctaatggcaa cctgttcagg tagcaatgag
      301 gtttcatttg caattttatg gatgactttg gaaacccgct tgtcggtttt gtttttgggc
      361 gataccaacc tgccaccacc tagtggtgac caagccatgg gaatgatatg cttttgcatt
      421 aaatagtcca gattcccatt ttgcaggtgt tcaacctgta atggtgatat ttcaatttgg
      481 tttgccacca ggtgaatggg tgtaaaggat tgcagcatct caacctggtg aggcaaaaag
      541 tttgaaaccc caaaatgcaa aaccttgccg gtactataaa gatgattgaa tgccaatgcc
      601 acctctcccg ggtttagtag tggcgatgga cggtgaagta gcaaaagatc gatatgatcg
      661 gttctgaaat tttttaggga tgtctctact gcgcttacaa tatgattata gctataatcg
      721 taatgtttta ctgttacttc agggaatttc ccggagatga gacggattcc gcatttggtg
      781 ataatttgta tgtgattacg aaccgaaggc tctagggcta gggcattacc gaaaagtttt
      841 tcgcaggagt agttgccata aatatcggca ctatctatcg aggtgatacc taagtcgata
      901 cattgttggg tgagataaag gagctcccgt tggctcaaat tccattcggt tagtcgccag
      961 tgaccccata ccaaaggcga aagcgatatc ctgtcagttt tcatttaccc gaggttaatc
     1021 aattttatag aacgatgaag catctttcag gttagcagca agttttagca ggtgctcgga
     1081 gtaggttcta aggtcgttag ctatatcgct ggtgagctta ctaacagctg agagtttttg
     1141 catggagtcg cctatccggt ttgtttcctg ctgttggtca tcaaattcaa acgagagctg
     1201 cttggcatgg tcggcaattc gattaacctc atgggctagc ttttccataa cgtttcgaac
     1261 ctcttccact gtggcaaggc ttgagtttgc cagatttact atatcctgag cagcctgttt
     1321 cgaccgttca gcaagtttac gcacttcgcc ggcaaccacg gcaaaaccac tgccgtgttc
     1381 acccgcgcga gcagcctcaa ccgctgcgtt aagggcaaga atattggttt gaaaggctat
     1441 atcgttaaca acctgaatct tattagcaac cattttcatg gcatcgagtg ctttggccga
     1501 tgtttggctg ccttgcttga tggtttcaac cgcttttgtt gatatgttaa gctgatcttg
     1561 taccgacttt gtgctctgcc caattgatcc aaccatccgc tgaatggaat gtgaaacctc
     1621 attggtggct aaggttaact caccaacagt tgacgttagg gcttcagagt tggtattcat
     1681 ggtttgtccg gtatccataa tgctttcgga aacctgtcca attcccctga acgtatcgcg
     1741 caacctggcg gccatgttgg taagtgattt cgagagagtt ccaatctcat catcggaatt
     1801 atcgttaaaa gctgcttgta agtttccttt gctaatttcc tgggcaaatt caaccccacg
     1861 agttacacgt ttcgatatnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnat
     1981 atggtgaata ctatcctctt cggcatttat ttccttcatt gtttttccca gaactgttgt
     2041 atcgggatgt gaaacgtaaa cactaccgtt cgatagcaga aatgcatagc tgttttcaaa
     2101 aagttttagc ttatcaactc gatctttgat ttctgtcagg gtgatgtcgc agccaacagt
     2161 acctaccaat tggttaccgt acgaaatagg gcttactagg cttgtcattg ggatttttac
     2221 cttatcggag ttggagtact tccagtagta aggttcaagt atggtgttgc cttttatatt
     2281 ttttgtaacc caaaggcttt tgctcgagag attatcaaaa gttacagtat cctgccgata
     2341 ttttagcaca tcattttccc agtaaaaggt acaccgcaat cgtcctttgc ttttgcctgt
     2401 tttatcaatc tttgataaat cccagttcac ccagagcgat tttatgttgt gatgctcttt
     2461 aagaacatcg gaaaccattc tgttttgcag gtcaatccaa tccttttggc ttgtattatt
     2521 gaatgcacga aaagcctcag ctagtgttct tgttatactc aattcctggt tgagctgatc
     2581 ctcaaaactg cgggcagttt ccttaaccag cgtgtttaca tagctatgag catccttaag
     2641 gttatcgtgc tgtatgctct taatgatgaa tacgaaaaca atcagatata caatggccga
     2701 gatgccaact aggaggatta gcatcttact ctgtaaacgc cttaaattta atggcttcat
     2761 ggtcaatgat ttaacactct gtgagatagg gaaatatcct tgccaaaata aatttttaac
     2821 ataacttaag caaataattc gatttttttt gtaatttgta aaatgcattt tttgacaggt
     2881 gaagaaaact ctaaagttta atatttaatg aaaaacagat taaattattt tcccatttac
     2941 attggtataa cctatactat tacgatgcta tcgtgcggta gagcattgcc cactaacgat
     3001 gatttaaagt ctgaaatatt agatgtagaa tcacagtttt gtgattatgt tatgcggcat
     3061 ggaattgctg aaggttttgc attttttgcc gatgaatctg caacaattct ttgtggtaga
     3121 gatagcttaa ttaaatggaa gaacgctatt taccattact attcagatac tcaatacaag
     3181 ggagctaaag tcgaatggaa acccgagttt gttcaagttt caaagtgtgg cgatatagca
     3241 tatacctata ctacacatgg aaggttccaa aaacaaatgc cgatacattg gttgtgcaag
     3301 gaatatttca tacggtttgg aagtgccatt ccgatggaac ctggaagtac gtttagaatt
     3361 aacttataaa agcacgatat atggaacctg aaaagcaatt ttggcaatcg tcgagcggga
     3421 aaaagctatt tgcctggcat ataaaacccg cagcaacacc caaagcaatt atcctgattg
     3481 ttcatggaca tggagaacat tcattgcgat atgttccctg ggcaaaacgg tttgcttacc
     3541 atggctttat agtccagtcg tgggatcata ttggtcatgg ccaatccgaa ggcagaagag
     3601 ggcatatcag gtactttgag cagttgcttt ttgaaattga tttggctatt accaaaatta
     3661 ctcacgatta cccaaagcta ccaataatac tctacgggca tagcatggga ggaaacattg
     3721 ccctgaacta tgccataaat agaccaacca aacttgacct tctggttgta acctcgccct
     3781 ggttgaaatt ggcagttcca ccatcaccgg taatggatgt acttagcaag tttatgaatt
     3841 tggttgcacc ctttattcaa attaagtcaa ctgtaaaacc tgatcaaatc tctcaaatcc
     3901 ctgaggaggt tgagaagtat gctaccgatc cactcaatca cgggcttatt actcctaggc
     3961 tttacacatc aatcacaagt tctaccaagt acatttttgg caatgcccaa aagataagta
     4021 ttcctaccct gctcatgcat ggaagcggcg atactattac ctcgcacaaa gctaccgatg
     4081 agctatcgca tataattcct aaggcaacct acattgaatg gagcggatgc tatcatgaat
     4141 tgcatcatga gcaggaaagg gatgaagttt ttaattcaat actgaactgg attaatgagc
     4201 agctctaaaa ttgatttccg taccgaggtt accgtaaaca atttcccttt ccgtattagc
     4261 taccaaacac ctgtgctgtt ctgcggttcg tgctttgccg agaatatagg tggaattatg
     4321 catagctaca agatgcctgt gcaggttaac ccgttcggtg tggtttacaa tcctttttcg
     4381 gttgcaaggg tgttgaggaa cattctgcaa aacaaggagt acaccagtaa cgatttacat
     4441 agcaggaatg gcttatggtt cagctttgat cattacaccc gattctcgtc agagagtaaa
     4501 gatgaatgct tgcttaaaat taatgaggct tgcagtagtg cccatgaatt cattaagaag
     4561 gcaaacttga taggagtaac atttggaaca gcacgtgttt acagacttgt ttctacgggt
     4621 aaggttgtgg caaactgcca caaaattcct gcaaaggaat ttacccatac actgctttcg
     4681 gttgacgaaa ttgtttcaga gtggacgaaa ctgatagacg aggttttggc ggtaatccca
     4741 aacattcggt ttctttttac agttagcccc atcaggcact ggaaggatgg cccaattggc
     4801 aaccagctca gcaagtcaac tcttattctg gctgttcaca ggctggtaga aatatactcg
     4861 gacaaagcat tctattttcc cggatacgaa ataatgatgg acgatttaag ggattaccgt
     4921 ttttacgatt ccgatatgct tcacccatcg caactggcta tcgattacat ctggcaaaaa
     4981 ttctctgatg cacttattga gcaccaggct atgcaagtaa ctgaaaaagt tcataggata
     5041 caacaggctt taagccatag accggttaat cccaaatccc aagaatttaa aaggtttgta
     5101 gatataattt tgaatgaggt aagtagcatt caaaagcaat acccctttct taactttaac
     5161 gaagaggtaa acaaattaaa aagttatata gtatgatttt attcaagagt ttaagagagt
     5221 tgctatatgt tttttctgta actgcctatt tgttttttgc tgctactaat gtcttttcgc
     5281 aggattccac atgggtttgg cgtgagaagg gtagcagtag ccttaagttt tttggtttaa
     5341 tagcgtatca ggaagggcgg ggtgagcagg ggtttggcat ccctttaggc ataggattcc
     5401 aaaagcatta cctaaacgga cgctggaggg taaatacctc catacaggca gcatcataca
     5461 ggacctttgc
//
LOCUS       DAOSAL010000117         5456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_9441_length_5456_cov_51.517867, whole genome shotgun sequence.
ACCESSION   DAOSAL010000117 DAOSAL010000000
VERSION     DAOSAL010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 5456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5456
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9441_length_5456_cov_51.517867"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            261..1637
                     /gene="hisS"
                     /locus_tag="PK049_11010"
     CDS             261..1637
                     /gene="hisS"
                     /locus_tag="PK049_11010"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOK62130.1"
                     /translation="MAKTKPSIPKGTRDFGPDQMVRRNYIFDTIRTVFSKYGYSPIET
                     PAMENLSTLLGKYGDEGDKLLFKILNSGDFIDKVEPSLLKPGNSNAASLAICEKGLRY
                     DLTVPFARFVVQYQNEITFPFKRYQIQPVWRADRPQKGRYREFYQCDVDVIGSNSLLN
                     EAELVLIINEVFGKLNVRTLLKVNNRKILSGIAEVIGHADKLVDITVAIDKIEKIGID
                     AVNAELRERGIDEVAIQKLQPIIQLQGSNAQKLSTLRNVLQGSPIGQKGIDEMEELFR
                     YIEAWGSLSTQVELDLSLARGLNYYTGAIFEVKAQDAEIGSICGGGRYDDLTGIFGLP
                     NVSGVGVSFGADRIYDVMQQLNLFPESLTSTTQVMFANFGTTEVAYCMPAARKLRDAG
                     ISVEIYPDAAKLKKQFEYANKRNIRFVVIAGETEIQNRQFSVKNMATGNQVTIPFDGI
                     EEHIKLNL"
     gene            1655..3154
                     /gene="hutH"
                     /locus_tag="PK049_11015"
     CDS             1655..3154
                     /gene="hutH"
                     /locus_tag="PK049_11015"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HOK62131.1"
                     /translation="MAKVHTISPKPLSWDVIENLIDEPHTLELSEESRQLIAKCRDYL
                     DRKMENEETPIYGITTGFGSLCNKTISKDELSQLQKNLVMSHACSTGDMVKPEIVKLM
                     LFLKAHALSLGHSGVQVETVQRIIDFYNNDVIPVVYDRGSLGASGDLAPLSNLFLPLI
                     GMGEVHYDGQIVSAADVLKKFGWKPIELKSKEGLALLNGTQFMSAHGVYAMVKAFKLA
                     KLADIIGAVSLEAFDGRIDPFHENIQNVRPHQGQIETARAFRKILDGSELIKRPKKHV
                     QDPYSFRCIPQVHGASKDAINYVAGVLLTEINSVTDNPTIWPDEDLVISGGNFHGQPL
                     ALAYDFLAIALAELGNISERRTAQLILGLRGLPEFLVANPGLNSGFMIPQYAAAAMVS
                     QNKQLCAPASVDSIVSSNGQEDHVSMGANAATKLLRVVDNLERILAIELFNAAQALEF
                     RRPAKSSPYIEEFIASYRSIVGFIEQDRIMYKEMDKTVEFLRQGKFQRE"
     gene            complement(3241..4044)
                     /locus_tag="PK049_11020"
     CDS             complement(3241..4044)
                     /locus_tag="PK049_11020"
                     /inference="COORDINATES: protein motif:HMM:NF025115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HOK62132.1"
                     /translation="MQSKNLIRSALLVAIVALAYYTYTQIVANSFNNLTFSADSVVFK
                     RGCLHPDSSCFRVVYRFPVTVGKGSSRIEKLIVSDFVGNMDEEIDRNDFAHLKDMLVK
                     NSISYDSSYVEFLKQFGTGVEWYIDVDFKQILRTNRLITFRYEQSSYTGGAHDMYGYH
                     YLNIDLKKSKILKLTDLFTDMDRFKAIAQKAFTVQYLNNPADKTEYWLADDGSLVLAN
                     EFGLTSKGLLLHYNVYEIACFARGDIELLIPYPDLKEILVDAYRKELMN"
     gene            4160..4858
                     /gene="radC"
                     /locus_tag="PK049_11025"
     CDS             4160..4858
                     /gene="radC"
                     /locus_tag="PK049_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOK62133.1"
                     /translation="MENSEKRLTIKDLAEDDRPREKMERKGASSLSDAELIAILLRVG
                     NANETAVDVAQRLLGQVNNSLNELARLSLHQFTKIKGIGRTKAITILAALELGRRRSA
                     EGPVNRERIFSSRSVIDIFQPALADLPHEEFWVLLLNKANRIIERVKVSQGGVGGTVV
                     DPKLVLKHAVERLASAIIVVHNHPSGNPQPSDKDIALTEKLKNAAQLFDITLLDHVIV
                     TDGECYSFADNGKM"
     gene            5059..>5456
                     /locus_tag="PK049_11030"
     CDS             5059..>5456
                     /locus_tag="PK049_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HOK62134.1"
                     /translation="MKRLIISAFMIVFTNASISQVSEMYKAEIFNSKGIQLPYRILYP
                     KNFNSTQKYPLVLFLHGSGERGSDNVKQLTHGAKLFASDSVMDKYPAIVVFPQCPAES
                     YWANVKIVNQSDGNRSFDFPAKGKPTPAMAA"
BASE COUNT         1521 a         1197 c         1237 g         1501 t
ORIGIN      
        1 caatcgagat tcctcggcat agtctatccc gatatatcgg gactcggaat gacaataggt
       61 aataattaga ttccgtttaa cggttcacgg ttaacgattc atgcacttct tctaacctcc
      121 tctaacttcc tgacattctc taaaaaactc gtttaacggc tcacggctca cggttaacga
      181 ttcatgcact tcctttaacc ttcattgcct ttttctccca aaaaaattgt aggtttgcag
      241 tcgtttcaaa cagcaacaca atggcaaaga caaaaccaag cattcccaag ggaactcgcg
      301 attttggtcc tgatcaaatg gttcggagga attatatttt cgataccatt cgtacagttt
      361 ttagcaagta tggttattcg ccaatcgaaa cgcctgccat ggagaacctt tcaaccctac
      421 ttggcaagta tggcgacgaa ggcgacaagc ttcttttcaa aattcttaac tcaggcgatt
      481 ttatcgataa ggttgagcca agcctactga aaccaggaaa ctcaaatgcc gcatcgctag
      541 caatatgcga aaagggtttg cgttacgacc ttactgttcc ttttgcccga tttgtggtgc
      601 agtaccagaa cgagattact ttccccttta aacgatacca aatacagccc gtttggcgtg
      661 ccgacaggcc acaaaagggt cgttaccgcg agttctacca gtgcgatgtt gatgttattg
      721 gttcaaactc gttgcttaat gaggctgagc tggtgctaat tatcaatgag gtttttggaa
      781 agctcaacgt gcgcaccctg ctcaaggtaa ataaccgcaa aatactaagc ggaatagccg
      841 aggttattgg tcatgccgat aagctggttg atattactgt agcaattgat aagattgaga
      901 agataggtat tgatgctgtt aacgccgaat tgcgtgagcg cggaatcgat gaggttgcca
      961 ttcagaaatt gcaacccata attcagttac agggcagcaa cgcccaaaaa ctttccacct
     1021 tacgcaatgt tttgcagggc agccccatag gccagaaggg aattgatgaa atggaggagc
     1081 ttttccgata cattgaggct tgggggagct tatcaacaca ggtggaactc gacctttcgc
     1141 ttgcgcgagg gcttaactac tacaccggtg ccatatttga ggtaaaagcc caggatgctg
     1201 agattggtag catttgtggc ggtggccggt acgacgatct aaccggtatt tttggtttgc
     1261 ccaatgtgag cggggtaggg gtttcgtttg gcgccgaccg catttacgat gtaatgcagc
     1321 agttaaatct tttcccggag agtcttacct ccaccaccca ggtaatgttt gcaaactttg
     1381 ggacaactga ggttgcatac tgcatgcctg cagcccgtaa gctccgcgat gccggaatta
     1441 gcgttgagat ttaccccgat gctgctaagc tgaaaaagca gtttgagtat gcaaacaagc
     1501 gcaacattcg ctttgtggta attgcaggtg aaaccgaaat tcaaaaccgt cagttttcgg
     1561 ttaaaaacat ggcaaccggc aatcaggtta ccattccttt tgatggcatt gaagaacata
     1621 tcaagttgaa cctataaaaa aaccaccatt aaccatggca aaagttcata ccatatcgcc
     1681 caagccctta agctgggatg taattgaaaa tttgattgac gagcctcata cccttgagct
     1741 ttccgaggaa tcgcgccagc ttatagccaa gtgccgcgat taccttgacc gtaagatgga
     1801 gaatgaggag acccctattt acggtattac cactggtttt ggttcactat gcaacaaaac
     1861 catatcgaag gatgaactct cacagctgca gaaaaatctt gttatgtcgc atgcctgcag
     1921 cacaggcgat atggttaagc ccgaaattgt aaagctgatg ctctttctta aggctcacgc
     1981 actatcgtta ggacattcgg gtgttcaggt tgaaaccgtt cagcgtatta tcgatttcta
     2041 caataacgat gtaattcccg ttgtttacga ccgtggctca ctaggagcat cgggcgattt
     2101 ggcgcccctt tcaaacctgt tcctgccgct aattggtatg ggtgaggtgc attacgatgg
     2161 ccaaatagtt tcggctgccg atgtactcaa aaaatttggc tggaagccca ttgaactgaa
     2221 gtcgaaggaa gggcttgccc tgcttaatgg tacgcagttt atgagtgcac atggcgttta
     2281 cgccatggtt aaggctttta agttggccaa gctagccgat ataataggcg cagtttcact
     2341 tgaggcgttc gatggtcgta ttgacccatt ccatgaaaat attcagaatg ttcggccgca
     2401 tcaaggacaa attgaaacgg cccgtgcttt ccgaaaaata cttgatggaa gcgagctcat
     2461 taagcgcccc aaaaagcacg ttcaggatcc ctactcattc cggtgtattc cgcaggtgca
     2521 cggagcctca aaggatgcca ttaactatgt ggcaggagta ttgctaaccg agataaactc
     2581 ggttactgat aacccaacca tttggcccga tgaggatttg gtaatttcgg gtggtaattt
     2641 ccatgggcag ccgctggcct tagcatacga tttccttgcc attgcccttg cagagcttgg
     2701 caacatctcg gaacgccgaa cagcgcagct tattcttggc ttacgcggtt tacctgaatt
     2761 tctggttgct aatcccggac taaactccgg ttttatgata ccacagtatg ctgctgcagc
     2821 catggtaagc cagaacaagc agctttgtgc tcccgcatcg gtcgattcca ttgtttcctc
     2881 aaacggccag gaagaccatg ttagcatggg tgccaatgca gccacaaagc tgcttagggt
     2941 ggtcgataac cttgagcgca tactggccat tgagctattc aatgcagccc aagctcttga
     3001 gttccgtagg cctgcaaaat catcgcctta cattgaggaa tttattgcca gctaccgttc
     3061 catagtaggt tttattgagc aggataggat aatgtataag gagatggaca aaaccgttga
     3121 attcctcaga cagggtaagt ttcagcggga gtaaataaga tttccttaaa aaaataaccg
     3181 acagctgttt tggctgtcgg ttttttcttt agttaagcta ttgtaaaaaa gacttgggtt
     3241 ttagttcatc aattcctttc ggtaagcatc aaccaaaatc tcttttaaat cagggtaggg
     3301 gattaacagc tcaatatcgc cgcgagcaaa gcaagctatc tcgtaaacat tgtaatggag
     3361 cagcaaaccc ttactggtta aaccaaactc attggcaaga accaaagagc catcgtcggc
     3421 caaccagtat tcggttttgt cggcaggatt attcaggtac tgcaccgtaa aggccttttg
     3481 ggcaatggct ttgaagcggt ccatatccgt aaacaggtcg gtcaatttta ggatttttga
     3541 tttttttagg tcgatattca ggtaatggta accatacata tcgtgggcac cgccagtgta
     3601 actcgattgc tcgtaacgga aagtaatcaa acgatttgta cgtaatattt gtttaaaatc
     3661 aacatcaatg taccactcaa cgccagtacc aaattgtttc aggaactcta cgtaggaact
     3721 gtcgtagctt atggagtttt ttaccagcat atcctttagg tgagcaaaat cgtttctatc
     3781 aatctcctca tccatgtttc ctacaaaatc gcttacaatt agcttttcaa tacgggagct
     3841 acccttacca acggtaacag ggaaacggta aacaacccta aaacacgatg agtcgggatg
     3901 taggcaaccg cgtttgaaaa caaccgagtc ggccgaaaag gttaaattgt tgaaactatt
     3961 tgccacaatt tgggtgtagg tatagtaagc cagggcaaca attgccacca gcagcgctga
     4021 gcgaatcagg ttcttacttt gcatagcgtt cttcgattag taacatcaaa tatattgcta
     4081 tttatattga ttttgataaa actgcttgct ttttatttcc gttctgttta acttatgtaa
     4141 cctattcaaa gccatggtca tggaaaactc cgaaaagcgg ttaaccataa aagatttggc
     4201 tgaggacgac cgtccacgcg agaaaatgga gcgaaaagga gcctcaagcc ttagcgatgc
     4261 ggaactgata gctattctgc tcagagttgg taatgcaaac gagaccgcag ttgatgtggc
     4321 ccaacggtta ctggggcagg taaacaacag tctcaacgaa cttgctcgtc tgagcctgca
     4381 tcagtttacc aaaataaagg ggattggcag aaccaaagcg attaccatac tggctgcgct
     4441 tgagctagga cgcaggcgga gtgctgaggg accagtaaac cgagaaagga ttttttcgag
     4501 ccggagcgta atcgatatat ttcaacctgc gcttgccgat ttaccacacg aggagttttg
     4561 ggttttgctt ctgaataagg ccaataggat aattgaaagg gtaaaggtga gccaaggcgg
     4621 agtgggaggc acagtggttg accctaagct ggtgctaaaa cacgccgttg agcggcttgc
     4681 ttcggctata atagtggttc acaatcatcc atcgggtaac ccccagccca gcgataagga
     4741 tattgcacta accgaaaagc tcaagaatgc tgcgcagcta tttgacatca ccctgctcga
     4801 tcacgtgata gtaaccgatg gcgaatgcta ctcttttgcc gataacggaa aaatgtaaat
     4861 attatctgct ggttaattgt taatcgttgt ttgtggatgc tactacattt acacccatac
     4921 accttgtaaa cactgtatga acaccttgtc gctttgagat actttttttg ttgatggtta
     4981 ctaataacca attactactg aaaacagcac attttaaact atgattcact aactttaccc
     5041 attataaatt ttgaggaaat gaaaaggtta atcatatctg ctttcatgat tgtatttaca
     5101 aatgcctcaa tttcgcaggt tagtgaaatg tataaggcgg agatattcaa ttctaaaggt
     5161 attcagttgc cctaccgtat tctgtatcct aaaaacttta attctaccca aaagtatcca
     5221 ttagttcttt tcctgcatgg ctctggcgaa cggggttccg ataatgttaa gcagcttacc
     5281 catggggcta agctgtttgc atctgattct gttatggata aataccccgc aattgtagtt
     5341 tttccgcaat gtcctgctga gagctactgg gctaatgtaa aaattgtaaa tcaatccgat
     5401 ggtaatcggt cctttgattt tccggctaaa ggtaagccaa ccccagcaat ggctgc
//
LOCUS       DAOSAL010000118         5410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_9555_length_5410_cov_49.814753, whole genome shotgun sequence.
ACCESSION   DAOSAL010000118 DAOSAL010000000
VERSION     DAOSAL010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 5410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5410
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9555_length_5410_cov_49.814753"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(375..1184)
                     /locus_tag="PK049_11035"
     CDS             complement(375..1184)
                     /locus_tag="PK049_11035"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOK62135.1"
                     /translation="MLEKINQTVTYIKSKVTIEPEVGIILGTGLGGLVREIENQQALE
                     YKDIPNFPVSTVEGHSGRLIFGTLGGKKVVAMQGRFHYYEGYDMKQVTFPVRVMKFLG
                     IKYLFVSNASGGVNPDFEIGDLMIINDHINLMPNPLLGPNINELGPRFPDMHKPYDHD
                     LIALAQKIGAELGFKLQIGCYVGTTGPTFETPKEYQYFRIIGGDTVGMSTVPEVIVAR
                     HMNLPVFAISIITDLGVPGKIVEVSHEEVQKVGAMAEQKMTRIFKEMLTRL"
     gene            complement(1141..2232)
                     /gene="lpxK"
                     /locus_tag="PK049_11040"
     CDS             complement(1141..2232)
                     /gene="lpxK"
                     /locus_tag="PK049_11040"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOK62136.1"
                     /translation="MSSNFISKTMQKLRLLLLPFSLLYWFAVKIRNLLFDINVLRSFE
                     FDLPTICVGNLTVGGTGKTPHTDYLISLLKNNYKTALLSRGYGRKTKGFRIVEQSSLA
                     VEVGDEPLQLKLKHPDVACAVHEDRLNGVVEVMGSFPETEVVVLDDAFQHRWIKAGLN
                     ILMVDYNRPVYADFFLPAGNLRDSISEIKRANIVIVSKCPDNLTTHEQKLIAQRIKIK
                     QNQDIYFTSVSYGKPKAVFSNYEDCNFNPNETIFALSGIANPKPFIHQLEKNYKLVGK
                     KTFADHHNFTVKEILSIFEVTSNDVMLVTTEKDAARIRGLNLPSDVKSRIYYLPLEVY
                     FINNNENRFNSQILNYVGENKPNSNLYQE"
     gene            complement(2225..3466)
                     /locus_tag="PK049_11045"
     CDS             complement(2225..3466)
                     /locus_tag="PK049_11045"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOK62137.1"
                     /translation="MSFESYIAKRFLSGSDSQSLSRPFIRITIASVALSVAVMIVAIA
                     VITGFKREIKQKVVGFGSHIQITNYDSNQSLETNPIPTNLPVIDEVKKVKGVRHIQRF
                     AIKAGIIKTDTDIQGVVLKGIDSDFDWTFFQKNLVDGAIFKITDSTTTTASLISKTLA
                     NLLKLKVGDTYDMFFVQEPPRYRRFTVAGIYDTKMVEFDKMFVLCDIKHIQRLNGWSQ
                     NQATGFEVLVDDIDRVDDLWVEVDDIAGFVFLDDGSRLKVQSIIDKYPNIFDWLNLQD
                     INAAVLIVLMLLVAAINMISGLLIIILEKTSTIGLLKSMGTSNRSVRKIFLIQSAFIM
                     IRGLFIGTAAGLILCFLQKQFGIVKLDEAFYFLSEVPINIVWWHVIVLNVLAFAVGVA
                     MLVVPSMVISRISPDKTLKFE"
     gene            complement(3485..>3811)
                     /locus_tag="PK049_11050"
     CDS             complement(3485..>3811)
                     /locus_tag="PK049_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H"
                     /protein_id="HOK62138.1"
                     /translation="QTGKLIFHQGPFPDATNNIGEFLAIVHGLAHLKKLNSEIPIYTD
                     SMTAICWVRAKKARTKLIQTERNKNLFELLERAEKWLRENTWKNPLLKWETQYWGEIP
                     ADFGRK"
     assembly_gap    3812..3911
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3912..5050)
                     /locus_tag="PK049_11055"
     CDS             complement(<3912..5050)
                     /locus_tag="PK049_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005936469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOK62139.1"
                     /translation="MRFGKIVLTLSVSVFLITARAQEILPGARLMSNYLPLVQGKRVA
                     VVANHTTLVSSKHLVDTLLSAGVAVKKIFSPEHGFRGDIEAGELIGNYTDKVTGLPVI
                     SLYGGSKKPKPADLSDVDVLIFDLQDVGVRFYTYISTMHYVMEACAENNKPLIILDRP
                     NPNGFYVDGPVLDTAYRSFVGMHPVPLVHGMTIGEFAQMINGEGWLKGKIQCNLTVIP
                     CKGYTHSMTTALPVRPSPNLPNHLSILLYPSLGLFEGTVVSVGRGTDYPFQVFGFPNF
                     PDKGFRYIPVEKRGASLNPPYKGKPVYGIDLRDYSVNYFLDRREIILDWLIYSYNNYP
                     EKDKFFNNFFNLLAGTSVLRQQIENGLTPSEIRATWQPALEQFKLL"
BASE COUNT         1501 a         1178 c         1116 g         1515 t
ORIGIN      
        1 gaaaaatatt tgcaagatct agtgaattag gcctataaac cttgcaatat ttttaacccc
       61 tatccgctat gagaattatg aaggcacaag gaattgccaa ctcattagta cagctgaact
      121 ccaaagtccc ctgaactatt ctctcctttt catttgcagg aagagtttta gaatacggct
      181 attgccgttt ttaatcatag gtagattttt tgcatttaca atgaaaatct atgtattagg
      241 atttttcgct agctctcaac atgacaatac actcacgaac aaccgtcaac catcaaccat
      301 caactatcaa ccatcaactt aataaaaaag gccaccctaa acaggtagcc tctccctgcg
      361 aatagcatta aggattatag ccttgtgagc atctccttga aaattcttgt catcttttgt
      421 tcggccatgg ctcctacctt ttgaacctcc tcgtgcgata cctcaacaat tttgcctgga
      481 acacccaaat cggtaataat ggatatggca aacacgggca ggttcatgtg acgggcaaca
      541 ataacctccg gaacggttga catacccact gtgtcaccgc caataatacg gaaatactga
      601 tattccttag gtgtttcaaa ggttgggccg gtagttccca cgtagcatcc aatctgcaat
      661 ttgaagccca gttcagcccc aatcttttgg gccaaggcaa tcaaatcgtg atcgtagggt
      721 ttatgcatat cggggaaacg gggtccgagc tcattgatat tgggacctaa gagcggattg
      781 ggcattaggt tgatatggtc gttaattatc atcaaatcgc caatctcaaa gtcggggttt
      841 acgcccccgc tggcatttga tacaaaaaga tacttaatac caaggaactt catcacccta
      901 accgggaagg ttacctgttt catatcgtaa ccctcgtagt agtggaagcg gccctgcatg
      961 gctacaacct ttttaccgcc aagggtgcca aatattaagc gacccgagtg tccttcaaca
     1021 gtggaaacag gaaagttagg tatatcctta tactccaatg cctgttgatt ttcaatttca
     1081 cgaactaagc cacccaaacc ggttccaagt ataatcccca cttcgggctc aatggtaacc
     1141 ttactcttga tataggttac tgtttggttt attttctcca acataattga gtatttgact
     1201 gttaaaccta ttttcgttgt tattgataaa gtaaacctca agcggcaagt agtaaatcct
     1261 actttttaca tccgatggca ggtttagccc acgaatacga gctgcatcct tttcggtggt
     1321 taccagcatt acatcgtttg aggtgacctc aaaaatagat agaatttcct taaccgtaaa
     1381 attatgatgg tcggcaaagg tcttctttcc taccaattta taattttttt cgagttgatg
     1441 gataaatggc ttgggatttg ctattcccga taaggcgaag atggtttcat ttggattaaa
     1501 attacagtct tcgtaattgc taaaaacagc ctttggctta ccatagctca ctgaggtaaa
     1561 ataaatatcc tggttttgct tgattttaat cctttgggct attaactttt gctcatgggt
     1621 tgtaaggtta tcgggacatt tgctcactat aacaatattg gcccgcttaa tctccgagat
     1681 gctgtcgcgg agattcccgg caggcaaaaa gaaatcggcg taaaccggtc ggttgtaatc
     1741 taccattaag atgttaagcc ctgcttttat ccaacggtgt tggaaggcat cgtccagaac
     1801 aacaacctcg gtctcaggga acgagcccat tacctccacc acgccgttta gcctgtcctc
     1861 atgcacggca caggcaacat cggggtgttt gagcttaagc tgaaggggtt catcgcctac
     1921 ctcaacggcc agcgacgatt gctcaacaat tctgaaacct ttggttttac gaccataccc
     1981 acggcttagc agtgccgttt tatagttgtt tttcagtaaa ctaataaggt aatcggtatg
     2041 cggggttttg cctgttcccc ccacggttaa gttaccaaca caaatggttg gaaggtcgaa
     2101 ctcaaagctg cgcagaacat tgatatcaaa aaggaggttc cgaatcttta ctgcaaacca
     2161 gtaaagcaac gaaaagggca aaagtaaaag acgaagcttt tgcatggttt tactgataaa
     2221 gttactactc aaatttaagt gttttatcgg ggctaatccg cgaaattacc atcgatggca
     2281 ccaccagcat agccactcca acggcaaagg ctaaaacatt caaaacaatt acatgccacc
     2341 aaactatgtt gatgggcact tcggatagga agtaaaatgc ctcatcgagc ttaactatac
     2401 caaactgctt ttgcaaaaaa caaagtatca aacccgctgc ggttccaata aaaagtccgc
     2461 gtatcataat gaacgccgac tgtatcaaaa atatctttct aacgctccgg ttcgatgttc
     2521 ccatcgattt caaaaggcca atggtagatg ttttttccag aataataatg agtaagcccg
     2581 aaatcatgtt aattgctgcc actagcaaca ttaaaacaat tagaacggca gcgttaatat
     2641 cctgcaggtt caaccagtca aaaatatttg ggtacttatc gataatgctt tgaaccttta
     2701 gccttgagcc atcgtcgagg aaaacaaagc ctgcaatatc atcaacctca acccataggt
     2761 catcaaccct atcgatatca tcaaccaaaa cctcaaatcc tgtggcctgg ttctggctcc
     2821 acccgttgag cctctggatg tgcttaatgt cgcaaagcac aaacatttta tcgaactcaa
     2881 ccattttggt atcgtaaata ccagcaaccg taaaccttct ataacggggt ggttcttgaa
     2941 caaagaacat atcgtaggta tcgcctacct ttaactttaa aaggtttgcc agcgttttgg
     3001 aaataagcga tgcagtggtt gtggttgagt cggtaatttt gaaaatcgcc ccatcaacta
     3061 ggttcttttg gaaaaaagtc caatcgaaat cggaatctat cccctttagg acaactcctt
     3121 gaatatcggt gtcggtcttt atgatacctg cctttatggc aaaccgctga atatgcctta
     3181 ctccttttac tttttttacc tcatcaatta ccggaaggtt tgttggtatg gggttggttt
     3241 caagcgattg gttactgtcg tagtttgtta tttgaatatg gcttccaaaa cccacaacct
     3301 tttgtttaat ctccctttta aagccggtaa taacggctat ggccacaatc ataacggcca
     3361 cgctcagggc taccgaggca atggtaatac ggataaatgg cctcgatagg ctttggctat
     3421 cggaaccgct taggaatcgt tttgctatgt agctttcaaa actcacttgt agttggcatt
     3481 ttgtttattt tctgccaaaa tcggcaggga tttcgcccca gtactgggtt tcccatttaa
     3541 gcaaagggtt tttccatgtg ttttccctta accatttttc ggcccgctct agtagttcaa
     3601 agagattctt attcctttcc gtttgaataa gtttggtacg tgctttcttt gccctaaccc
     3661 aacatatggc agtcatggag tcggtgtaaa tgggtatttc gctattcagc tttttcagat
     3721 gcgctaaccc atggacaatg gcaaggaact cgcctatgtt attggtggcg tcggggaaag
     3781 gtccttgatg gaaaatcagc ttgccggttt gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nagcagtttg aactgctcaa gcgctggctg ccaggtggct ctaatttccg
     3961 aaggggttaa cccgttttct atttgctggc gcagcacgct agttccggca agcaggttga
     4021 agaagttatt gaagaattta tctttttcag ggtagttgtt gtaggagtat attagccaat
     4081 cgagaattat ctcgcggcgg tccagaaagt agtttaccga gtagtccctt aggtcgatac
     4141 cgtaaaccgg tttgcccttg tagggcggat tcaagcttgc cccacgtttc tcaaccggta
     4201 tgtacctaaa acccttatcg gggaaattag ggaagccaaa aacctggaag gggtaatcgg
     4261 ttccacggcc tacgcttacc accgtcccct caaacagccc aagcgaaggg taaagcagaa
     4321 tggaaaggtg attggggagg ttaggggagg ggcgaacggg tagggcagtg gtcatgctgt
     4381 gggtgtaacc cttacaggga ataactgtga ggttacactg aattttcccc tttaaccagc
     4441 cctcaccgtt tatcatttgg gcaaactcac caatggtcat accgtgaacc aggggtacag
     4501 gatgcattcc cacaaacgag cggtaagcgg tgtcaagtac tggcccatca acgtaaaaac
     4561 cgttagggtt tggcctatcg agtattatca acggcttgtt attttccgcg caggcctcca
     4621 ttacgtaatg catagttgag atataggtgt aaaaccttac tccaacatcc tgcaaatcaa
     4681 aaattagaac atcaacatcg ctcaggtcgg ctggtttggg ttttttactt ccgccataca
     4741 gcgaaattac aggtaatccc gtaaccttat cggtatagtt tccaataagc tcgccagcct
     4801 caatatcgcc ccgaaagcca tgctccgggc taaatatttt ttttacggct acaccagctg
     4861 aaagcagagt atcgaccagg tgcttcgaac taaccagcgt ggtatggttg gccacaacag
     4921 ccacccgttt accctgaact agcggaaggt agttgctcat caatcgagca ccgggcaaaa
     4981 tttcctgtgc ccttgcagtt atcagaaata ctgaaaccga aagggttaat actattttac
     5041 caaacctcat acctcccttt tgcaaaatat gcgccaatta tggttttgct aaggtagaaa
     5101 ataatttata cagaaaaaag aaagggggag aaccatgctc tgcataaaca actctacggg
     5161 aggctgagtt tagctgaaaa attcagaacc ggtaaaacgt aattaaggat aaaggttaaa
     5221 ggataaagga taaaatggtg aaatatctca aagtgaattt tttagatttc attgattttt
     5281 actctcgttt taaaatgatg gattatctat caatgttgct tagttaagtt tatttgtcat
     5341 gttgcgcgca agagaaacat tatgctgtct tgctgagtcc tgatttatag ggatagactt
     5401 tgccgaagca
//
LOCUS       DAOSAL010000119         5306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_9792_length_5306_cov_49.520663, whole genome shotgun sequence.
ACCESSION   DAOSAL010000119 DAOSAL010000000
VERSION     DAOSAL010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 5306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5306
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9792_length_5306_cov_49.520663"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1611
                     /locus_tag="PK049_11060"
     CDS             <1..1611
                     /locus_tag="PK049_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK62140.1"
                     /translation="CYDFSQFDNIKVEPISPKLVVPVVKSTISFNDIVEREDANTIIF
                     TKPGDTRFFIAFRDTMDLFNAADRYSFPDFNFTKQYQLDAGEVPGVPVPAGQTWGPFT
                     KQYTETYNSIAGAEVKLVKLSAGNINMVITNNFQNSISAIIRFPSIRDQLGNPLVFAM
                     NTTLPSQTYTNNQDLTDYTLNLTSGLLYNTLTVEVELTIHSLGNPITLSDDANIQVSL
                     TGLDFDYLQGKLNETFPLNDINLTLDAFRTSIDADFSLSEPKLTMTFENLFGIPLAFS
                     TVNFDASNTNTSKQVSLVNEGVSPTGSLLLTQPNNIKYLTSLTQQQPEVDLKYVDRNN
                     SNLEDFLAIAPNEILISPTVTVGDPTTNHDYFVRKTSTLRMINEIEFPLAGWVDNLEI
                     CDTLEVELPDLEKDLKLVNDNALKIRLKFKFINSIPFNIYIQGYFLDDANTLLTQLYD
                     EASELMLVKSSAINPTTGKTSTPTTHWAYIDISKSKYDQMKNATNLVIQARMQTGGNT
                     HQNVVVESTNTLETMFSVELEGNVDLNN"
     gene            1634..2980
                     /locus_tag="PK049_11065"
     CDS             1634..2980
                     /locus_tag="PK049_11065"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HOK62141.1"
                     /translation="MKRYIFSTFILFTSLLAKAQPEVTLPYMRNVFQASFVNPTVMPE
                     HTFSMGFSVFGQAISNGFQPKSFMNYRSDTMYVNLNSLLGDMNDKNLIYVGENVDIFH
                     IRVKGKSSFTWFAMRQNATVTLQYPRELFSLAIEGNKQFVGSTLDLSNLKSDVSVYNE
                     YTIGYLKDLPQWTFAGRVSLLQGLANISFNPKVMQVEIDTAMYAHTATADGQLRTSGT
                     PKNSEGDISFDNVDDQWVTNRLTGFGNMGFAISGGATYKFDERMKLSFGFSDIGFIKW
                     KDEIQTYTLKGTTKFEGLDILGDFLRGNDVDVDSLLQKMEDDFTRDTVAVSYTTWLNP
                     KFNFSATYDLTRRTTLGLTFATFYNKRLYPSITLGVSQGIGRFFQLVGTASIYQRSIP
                     NLGVGIVIKPGPLQLFVVADNLYLAIDPLAFTNANVRVGINFVFGRVNPPQGLPYR"
     gene            3031..>5306
                     /locus_tag="PK049_11070"
     CDS             3031..>5306
                     /locus_tag="PK049_11070"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOK62142.1"
                     /translation="MKLKVVSRILITLSIISFGSKVFAQTGFTPQKLSGEINTGYAEI
                     NPILSPDGKTLYFTRVNHPENRFGEVDSQDIWCTTLNPDGTWSEAKRLPNTINLGRYN
                     AIFGILDDGKTFVINGHYTESGKYWTERGLSVIERIDENTWGTPRPLNVKGFTRMNKG
                     LTTTAYLTPDGKYLLTSFSKRAGGKNHSIYLSVKEGDGYSKPQKVKIGDGNLGESYEA
                     PFLSKDGNALFFSCKVDGNNNIYMANRTDDTYLNWSTPTALNDTINTPGWENYYRLNA
                     KESWAYYCSSSSKKEHSEIMRVKIYEEFPYVKLTGLVMNKAEESLMLADTGYNILVNG
                     KKSDSLKIDRASASYELLIPFGKKHSLLPELANWVGIPDTLDLTAVKEYSEMSKNLYL
                     QPIPLVRIYGKIINTRTGLPINPEMKYQILVNGVTSDSVKYEAEIAKYGMTLPLGNRY
                     ILSLNLPNFTSKTDTIDVTNAKYYTEKQVDFYATTVPWVEVNGVALDNSTFTPIIGAS
                     APKLKINGSTADSVSIDPVTGEFKVRLPFGKKYVTAIESKDYNPLDNQLDLTGYVEFT
                     QVKHEVYAERKDANMAILSGKVINLKTGQPLEKEVPVKLKVNGTETRGFIYDSTSASY
                     TLKLPVGVSYDVLPSVKNFYNKYEQVDLTKVKKGTKIAKNFYVTPIEVGQSVNIDFIY
                     FDVGKATLKPQSFRSLNALVEFLNEYPNVIVEIGGHTDNTGSAVVNQRLSDARAKSVA
                     DYIIKMGIPKDRITSKGYG"
BASE COUNT         1652 a         1109 c         1089 g         1456 t
ORIGIN      
        1 tgttacgatt tttcgcagtt cgataacatc aaggttgaac ccatttcacc taaattggtt
       61 gtcccggtgg ttaagtcaac catttcgttc aacgatattg ttgagcgaga ggatgctaat
      121 acaataatat ttaccaaacc tggcgatacc cggtttttta tagcattcag ggatactatg
      181 gatttgttta acgcagccga taggtattcg tttccggatt tcaactttac caagcagtat
      241 cagctcgatg caggtgaagt gccaggtgtt cctgtgcccg caggacaaac atggggacca
      301 tttacaaagc aatacactga aacatacaat agcattgctg gagctgaagt aaagttagta
      361 aagctatcgg ctgggaatat aaacatggta ataaccaata atttccagaa cagcattagc
      421 gcaattatcc ggttcccttc tatacgcgac caattaggga accctctagt ttttgccatg
      481 aatactactc ttcccagcca gacttacacc aataaccaag acctaacgga ttacactcta
      541 aaccttacta gtggtctctt atataacacc cttacagtgg aggttgaatt aaccatacat
      601 tcgttgggta atcctataac tctatcagat gatgctaaca tccaagtttc ccttacagga
      661 ttagattttg attacttaca gggtaaacta aatgagacat ttcccttgaa cgacatcaat
      721 ttaaccctcg atgcttttag aacatcgatt gatgccgact tttccctttc ggaaccaaag
      781 cttaccatga cttttgagaa tttatttggc ataccacttg cattcagcac tgttaacttt
      841 gatgcctcaa atactaacac cagtaaacag gttagccttg tgaatgaggg agtatcacca
      901 accgggtcgt tgctccttac tcagcccaac aacattaagt atttaacctc gttaacacaa
      961 cagcaacccg aggttgactt aaaatacgtt gatcgtaaca attcaaacct tgaggatttt
     1021 ctggccatag cacccaatga aatcctaatc agcccaacgg taaccgttgg tgatcccact
     1081 accaatcacg attactttgt tcgcaaaact tcaacactaa gaatgataaa cgagattgaa
     1141 tttccccttg ccggctgggt tgataattta gaaatttgtg ataccctaga ggttgaacta
     1201 cccgatttgg aaaaggattt aaaacttgta aacgataacg ctcttaaaat aaggttgaag
     1261 tttaagttta tcaatagcat tccatttaat atttatattc aaggatactt tctggacgat
     1321 gctaacacat tacttaccca gctttacgac gaggcttcgg agctgatgct ggttaaaagc
     1381 tctgcaataa atccaacgac aggtaaaaca tcaaccccca caactcattg ggcttacatc
     1441 gatataagca agagcaagta cgatcaaatg aaaaacgcaa ccaatctggt tattcaagcc
     1501 cgaatgcaaa ccggaggtaa cacccaccaa aatgtagttg tggaatcaac caacaccctt
     1561 gaaacaatgt ttagcgttga gttagagggt aatgtggatt taaataacta gtattctgaa
     1621 atttatgact ccaatgaaac gatatatttt ttccacattc attttattta cctcactgct
     1681 ggcaaaagcc cagcccgagg taaccctgcc atacatgcgt aatgtgtttc aggcatcgtt
     1741 tgtaaaccct accgtaatgc ccgagcatac ctttagcatg ggatttagcg tatttggtca
     1801 agccataagc aatggttttc agccaaagag tttcatgaac tatcggagcg atacgatgta
     1861 tgttaacctt aacagccttt tgggcgatat gaacgataaa aatttaatct atgttgggga
     1921 aaacgtcgat atctttcata tacgtgtgaa agggaaatcg tcctttacct ggtttgccat
     1981 gcgtcagaac gcaacagtaa cactccagta cccacgtgaa ctttttagct tagctattga
     2041 ggggaataag cagtttgttg gctcaacctt ggatttatcc aacctaaaat cggatgttag
     2101 cgtttacaac gagtacacca ttggatacct taaagattta ccccaatgga cttttgcagg
     2161 aagggtaagt ttgctgcaag gtttagccaa tatcagtttt aatcccaaag taatgcaggt
     2221 tgaaattgat actgccatgt atgctcatac tgctactgcc gacggtcaac tccgtacctc
     2281 gggcacacct aaaaacagtg aaggcgatat cagttttgat aatgttgatg accaatgggt
     2341 taccaatagg cttactggtt ttgggaacat ggggtttgcc attagtggag gcgcaacata
     2401 caagttcgat gaaaggatga aactatcctt tgggttttcc gatataggat ttataaaatg
     2461 gaaggatgaa attcaaacat atacccttaa aggaaccacc aaatttgaag gtttagatat
     2521 tcttggcgat ttcctgagag gaaacgatgt tgacgttgac tcgttactac agaaaatgga
     2581 agacgatttt acccgcgata ccgtagcggt ttcctacacc acttggttaa atcctaagtt
     2641 taacttttcg gccacttacg atttaacccg aaggacaacc cttggcctta cctttgcaac
     2701 attttataat aaacgattat acccttcaat aacgcttggt gtttctcagg gcattggccg
     2761 cttcttccag ctagtaggaa cagcttcaat ttaccaaaga tccataccca acctgggggt
     2821 aggaattgta attaaaccag ggcctttgca gctatttgtt gtagccgata atctgtacct
     2881 ggcaattgac ccgctggcat tcaccaacgc caatgtgagg gtaggaatca attttgtttt
     2941 tggcagggtt aatccacctc aagggttgcc ttaccgctaa ttttccaaat accgattatt
     3001 cagcaattat tcaatttaaa ggtaaaagcg atgaaactaa aagttgtaag cagaatcctc
     3061 ataacactca gcataattag ttttggttcc aaggtgtttg cacaaaccgg ttttactcca
     3121 caaaagctaa gtggcgaaat taatacaggg tatgctgaaa tcaatccaat actaagtccc
     3181 gatgggaaaa ccctttattt cacaagggta aatcaccccg agaataggtt tggcgaagtc
     3241 gatagtcaag atatttggtg cacaacattg aaccccgatg gcacatggag cgaagctaaa
     3301 cgcttaccca ataccattaa ccttggtcgt tacaatgcaa tttttggcat actcgatgat
     3361 ggtaaaacgt ttgtaatcaa tggccattac actgagagtg gaaagtactg gacagaaaga
     3421 ggcctatcag taattgagag gattgatgaa aacacatggg gtacgcccag gccgttgaat
     3481 gtgaagggat ttactaggat gaacaagggt ttaacaacaa ctgcatacct tacccccgat
     3541 gggaaatatt tacttacgtc gttcagtaag cgtgccggtg ggaaaaatca ttcaatctac
     3601 ctttcggtaa aggagggcga tggatactct aagccccaaa aggttaaaat tggtgatggg
     3661 aacctcggcg aaagctatga ggcgccattc ctaagcaagg atggcaatgc gcttttcttc
     3721 agctgtaagg ttgatgggaa caacaatata tacatggcta accgaaccga cgatacttac
     3781 ctaaactggt cgacgcccac tgccctgaac gataccatta atacaccggg ttgggaaaac
     3841 tactaccgtt taaacgctaa ggaaagttgg gcctactact gctcatcaag ttcaaagaag
     3901 gagcattcgg aaattatgcg ggtaaagatt tacgaagagt tcccctacgt aaagttaacc
     3961 gggcttgtaa tgaacaaggc cgaggagagt cttatgcttg ccgataccgg atacaacatt
     4021 cttgttaatg ggaaaaagtc ggatagctta aaaattgata gggcctcagc ttcgtatgag
     4081 ctacttattc cctttggaaa aaaacattcc ttacttcctg agctggctaa ctgggtgggc
     4141 atccccgata cattggattt aactgccgtt aaggaatact ctgaaatgtc aaagaattta
     4201 tacctgcagc ccataccatt agtaaggatt tacggtaaga taataaatac tcgaactgga
     4261 ctccctatta accctgaaat gaaataccag attttggtaa atggggttac aagcgattcg
     4321 gtaaagtatg aggccgaaat agccaaatac ggtatgaccc taccattagg aaacaggtat
     4381 atactctccc ttaacttgcc caactttaca agtaaaaccg atactattga tgtaaccaat
     4441 gcaaagtact atacggagaa acaggttgat ttttatgcca caacagtacc ctgggttgag
     4501 gttaatggcg ttgcgctcga taattcaacc tttacgccaa taattggggc ttcagcacca
     4561 aaacttaaga ttaatggttc aactgccgat agcgttagca tcgatccagt aaccggtgag
     4621 tttaaggtac gattgccttt tggaaagaag tacgttacag caattgagtc aaaggattac
     4681 aatccgttag ataatcagct cgatttaacc ggttacgttg agtttaccca ggttaagcat
     4741 gaggtttacg ctgagcgtaa agatgctaac atggcaatac taagcggtaa agttattaac
     4801 ttgaagacag gacaacccct tgaaaaagag gtgcccgtga agctgaaggt aaacggaact
     4861 gaaaccagag gatttattta cgactccaca tcggcaagct ataccttgaa gttgccagtt
     4921 ggtgtgtcat acgacgttct gccttccgtt aaaaactttt acaataagta cgagcaggtt
     4981 gacctaacca aagtgaaaaa gggaactaaa attgccaaga acttttacgt aactccaatt
     5041 gaggttggtc aatcagttaa tattgacttc atctactttg acgttggtaa agcaaccctg
     5101 aaaccacagt cgttccgctc acttaacgcc ctggttgagt tcctaaatga ataccccaat
     5161 gtaattgtag aaattggcgg acataccgat aatacagggt cggctgttgt taaccagcgg
     5221 ctatccgatg ccagggcaaa atctgttgcc gattatatta taaagatggg tattcctaag
     5281 gatagaatta catccaaggg atacgg
//
LOCUS       DAOSAL010000120         5092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_10307_length_5092_cov_50.824499, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000120 DAOSAL010000000
VERSION     DAOSAL010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 5092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5092
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10307_length_5092_cov_50.824499"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..445)
                     /locus_tag="PK049_11075"
     CDS             complement(11..445)
                     /locus_tag="PK049_11075"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HOK62143.1"
                     /translation="MVKSIKFRVIDFADDGYHWLVECEVNNADYWLVVDTGATRTVID
                     QSVIGEAEIRNHPQGLVVGFGSQTNSINTATLHSVWIGGIEFPEFEVAVADLSNLIEF
                     YSQMANVKLAGILGCDFLMRNATSMNINRKRIYLKPVKGENE"
     gene            671..934
                     /locus_tag="PK049_11080"
     CDS             671..934
                     /locus_tag="PK049_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK62144.1"
                     /translation="MITVHNVALRLERVNIWLSQFDLNQNSTYNDSQFRNSIKHLLTE
                     VRDIRQVIVKTKNEVALYSLDGARNLTVHYSQSETLLKESKMA"
     gene            957..1142
                     /locus_tag="PK049_11085"
     CDS             957..1142
                     /locus_tag="PK049_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK62145.1"
                     /translation="MREGNSLVASLVERVNPDLAMWIGVELKFPLGENIKSNLSASQR
                     IELSKPVSVPVYWDFFR"
     gene            1188..1301
                     /locus_tag="PK049_11090"
     CDS             1188..1301
                     /locus_tag="PK049_11090"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOK62146.1"
                     /translation="MSVRTVESHKNQIMRKLELTTTVDLVKYAIKNGIIDL"
     gene            1438..2370
                     /locus_tag="PK049_11095"
     CDS             1438..2370
                     /locus_tag="PK049_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOK62147.1"
                     /translation="MALKNQRKDLIRIGVFYDGNYFLHVSNYYNYFHQRKKRLNIAGL
                     HEFIRHMVANEEGVDPNFCQLIEAHFFRGRISAQEASQRGNQLYNDRVFDDILMSEGV
                     ITHYLPIRPRGGRREDKGIDVWLALEAFEQTLYKRFNVLVLITSDGDYVPLIRKINSL
                     GTKVMVLAWDFDYTDDQGREVTTRTSQELLREVTYPLPMHELINDGLKRKDKVVENLF
                     AVQDYSQPRQATKETITDEETKRARILSVNNGYGFIQYPPNNLFFHYQDVEDGEFEEL
                     IEGDMVEFVIGINEKGQEVAKRVRKVPDYDVNTW"
     gene            complement(2408..2653)
                     /locus_tag="PK049_11100"
     CDS             complement(2408..2653)
                     /locus_tag="PK049_11100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOK62148.1"
                     /translation="MVEIKVLGIGCPNCHKLEAMCHEVVNELGIDAHIESVTDMNQFM
                     DYGVLLTPALVINNEVKVQGKLPLKPTLVNWIKQGDK"
     gene            complement(2673..2903)
                     /locus_tag="PK049_11105"
     CDS             complement(2673..2903)
                     /locus_tag="PK049_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK62149.1"
                     /translation="METYICKACNLKFDANGTKTEWIDAAFGPCSKLIAKCPECGNEC
                     DEFRIPKQGSTDSYQHASHKPACDSCCGGCGI"
     gene            complement(2914..>3642)
                     /locus_tag="PK049_11110"
     CDS             complement(2914..>3642)
                     /locus_tag="PK049_11110"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOK62150.1"
                     /translation="TSPTCNARCVGCISKQDKSESPVHESQHRLTFVPTVDEIVGVAV
                     PHLETATNPVVSFGQGCEGEPLMQWELLRDAIKAIRAKTSKGIINLNTNGSRPKAVEE
                     LFRAGLNSIRVSMNSAQEKWYNLYIRPVNFTQADAVESLRIAHRFNAWSSINYFVMPG
                     ITDTAEELNALKVIIESARINMIQWRNFNIDPDWLFSRIEFNGQGEPLGVNQIMEQIR
                     ILYPWVYFGYFNPPLNIIEQYQFK"
     assembly_gap    3643..3742
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4007..>5092)
                     /locus_tag="PK049_11115"
     CDS             complement(4007..>5092)
                     /locus_tag="PK049_11115"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOK62151.1"
                     /translation="NWNARLIAITGPRGVGKTTLMLQKIKIENKIDESLYVSLDNIYF
                     SNHTLFELAGYFYRYGGRHLFIDEVHKYETWSQEIKNIYDSFPDLQIVFSGSSILDIY
                     KGFGDLSRRVVPYHLNGLSFREYLIFETGQDSEAYSLDQILSQKVKIKLENPLIHFKK
                     YLNHGYYPFYKDDDFMIRLTGTVNAVLEVDIPKYLDLKVSTIDKLKHLLQIISESVPF
                     KPNYSKIADLIGVSRNLLPDYLAYLEKAGLILELTNETKGIRRLGKVNKVYLNNPNLA
                     YMLSGENANIGNIRETFFFNQLKLNHKVFSSETVDFKVEGNEFEIGGKSKGNKQISKL
                     SNAYIVKDDIEYGFGNTIPLWHFGLLY"
BASE COUNT         1420 a         1047 c         1018 g         1507 t
ORIGIN      
        1 cctttagctg ttactcattt tcacctttaa caggtttcag gtaaatgcgt ttcctattaa
       61 tgttcatact tgtagcatta cgcatcaaaa aatcgcaacc caaaatgcct gccagcttaa
      121 cgttggccat ttgcgaatag aactcaatga ggttgctcaa gtcggcaacc gccacttcaa
      181 attcggggaa ttcaattcct ccaatccaaa ctgagtgtag ggttgctgta ttgatactgt
      241 tagtttgtga cccaaaccca actaccaaac cctgagggtg atttctaatc tcagcttcgc
      301 ctattaccga ctggtcaatc acagttcggg tagctccggt atcgaccact aaccagtaat
      361 cggcattgtt tacttcacac tcaaccaacc agtggtagcc atcgtcggca aaatctatca
      421 ccctgaactt tatgctttta accatgctgt gcagctaaaa tttttcagct gaaggtattg
      481 aattatttca ttgtttccgt aaattacagt gccaaaataa tttgcttaaa ccatgagcta
      541 tcggaatgct ttattcaaaa atcttttaaa atactcattc caacgccatt accttttcct
      601 atttgtttta atatttgcag ttttactttt tgggtaggca agcaagttga gccgagcaaa
      661 ggaatttaaa atgataaccg ttcataatgt tgccttaagg cttgaaaggg taaacatttg
      721 gctatcgcag tttgacctga atcaaaatag cacatataac gattctcaat ttcgaaattc
      781 aataaaacac ttgcttactg aagttaggga catccggcag gttatagtaa aaaccaaaaa
      841 cgaagtagct ttatactcac ttgatggtgc tagaaattta acagttcact atagccaaag
      901 cgaaactttg ctgaaggagt caaaaatggc ttagcgatga aacccttttt aaacttgtga
      961 gggagggcaa cagtttggtt gcatcgcttg ttgaacgggt taatcccgat ttagcgatgt
     1021 ggattggtgt tgagctaaag ttcccgctag gtgaaaacat caagtcaaac ctaagtgcca
     1081 gccagcggat tgagttaagc aagccagttt cagttcctgt ttactgggac ttttttagat
     1141 aatcagccca agccaagcat tggcaaaata ctaattagga cttttttatc agcgtcagaa
     1201 cagtggaatc gcataaaaat caaatcatgc ggaagctgga gctaaccact actgtcgatt
     1261 tggttaagta tgccattaaa aatgggatta tcgacctgta aacgtttgca ttaagatatc
     1321 gcattattta gatatatttt ttttgtgtga ttgctaaaga attatatttg cagcctcttt
     1381 gcactcaaag agactagcag ctaattaagt taattaacaa tccaaaatct attaaacatg
     1441 gctctaaaaa accagagaaa ggatttgata cgcattggcg tgttctacga cgggaactac
     1501 ttcctgcatg taagcaacta ctacaactac ttccatcagc ggaaaaagag gttgaatatt
     1561 gccggtttac atgagtttat tcggcatatg gttgccaacg aggagggtgt agaccctaat
     1621 ttctgccagc tgattgaggc acattttttc cgtggccgca tcagtgccca ggaggcaagc
     1681 cagcgtggaa atcagctcta caacgacagg gtattcgatg atatcctgat gtccgaaggg
     1741 gttattaccc actaccttcc cattcgcccg cgtggtggac gccgcgagga taaaggaatt
     1801 gatgtttggc ttgcccttga agcctttgag caaacgctct acaagcgctt taacgttctg
     1861 gtgcttatca cctccgatgg cgactacgtt ccgcttatcc gcaaaatcaa ctcgttggga
     1921 actaaggtta tggttttagc gtgggatttt gattacaccg acgaccaagg ccgcgaggta
     1981 accactcgca cctcgcagga gttgcttcgt gaggttactt accccctacc catgcacgaa
     2041 ctcattaacg atgggttaaa gcgtaaggat aaggtggttg agaacctctt tgcggttcag
     2101 gactacagcc agccccgaca ggcgactaaa gaaaccatta ccgatgagga aaccaagcgt
     2161 gcacgcattt taagtgtgaa caatggctac ggtttcattc agtatccgcc caacaacctt
     2221 ttcttccact accaggatgt tgaagatggt gagtttgagg aactcattga gggcgatatg
     2281 gttgagtttg taattggcat caacgagaaa ggccaggagg tagccaagcg agttcgcaag
     2341 gtgcccgatt acgatgtgaa tacttggtag aatttacact gaatgcctgc ttttgcaggc
     2401 attcatgcta tttgtcccct tgtttaatcc agtttacaag agtgggctta aggggtaatt
     2461 tcccctgaac cttaacctcg ttatttataa ccagggctgg ggttagtagc accccataat
     2521 ccataaactg attcatatcc gttacgctct caatgtgagc atcaatcccc agctcgttta
     2581 ccacctcgtg gcacatagcc tccagcttgt ggcagtttgg gcagcctata ccaagaactt
     2641 taatctcaac catatcattt tagcttttag cgttatattc cacatccacc gcagcaggaa
     2701 tcgcaggctg gcttgtgtga agcatgttgg tagctatcgg ttgaaccctg cttgggaata
     2761 cggaattcat cgcactcgtt cccgcattct gggcactttg ctatcaactt actgcatgga
     2821 ccaaatgcgg catcaatcca ttcggtttta gtcccgttag catcaaactt caaattgcaa
     2881 gccttacaga tgtacgtttc catagatcta tatctattta aactgatact gttcgataat
     2941 gtttaagggc gggttaaagt acccaaagta aacccagggg tagagtatac gtatttgttc
     3001 cattatttga ttcactccaa ggggttctcc ttgcccatta aattcaatgc ggctaaaaag
     3061 ccaatcgggg tctatgttga agttgcgcca ctgtatcatg ttaatacggg cagattcaat
     3121 aattactttc agcgcattca gctcttcggc tgtatcggta atacccggca ttacaaagta
     3181 gttgattgac gaccatgcgt taaaccggtg ggcaattctg agcgactcaa cggcatcggc
     3241 ttgagtaaag tttaccggac ggatgtagag attgtaccat ttttcctgag cagagttcat
     3301 gcttaccctg atgctgttaa gtccagcccg aaaaagttcc tcaactgctt tgggcctact
     3361 gccattggta ttcaggttaa taataccttt tgaggtttta gctctgatag ccttaatggc
     3421 atcgcgtagc aactcccact gcatcagtgg ttcaccctca cacccttggc caaaacttac
     3481 caccgggtta gttgctgttt cgaggtgtgg aacggctact ccgactattt catcgacagt
     3541 aggcacaaag gtaagcctat gctgcgactc gtgtaccggc gattcacttt tgtcctgttt
     3601 ggaaatacat cccacgcagc gtgcgttaca ggtaggcgag gtnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnagttcgct gccatagggc aggggtatca tttcatcgag
     3781 gtaaaggggg taaacacggt ttcccgagcg tcccgctgca aatagtttgg gattttcata
     3841 aatatttccc ttgccatcgc tgtaaacggc aaagggttga atacgtcgag tcataacttt
     3901 tgttattggc aacaaaggta gtaaacattt taccattagc cgattttgaa tacaaagggg
     3961 aaatagttag tgtttagaaa tggggattgg ggtaaaatag ccattactaa tacaacaaac
     4021 caaaatgcca aagcgggatg gtatttccaa aaccatattc aatgtcgtct tttactatat
     4081 aggcattgga aagtttggaa atctgtttat tgcctttgct cttgccacct atttcaaatt
     4141 cattaccttc taccttaaaa tctactgttt cagaagaaaa tactttgtga tttaatttaa
     4201 gttgattgaa gaaaaatgtt tctctgatat tgccaatatt ggcattttca ccacttagca
     4261 tgtaggctaa gttcggattg ttcaggtata ccttgtttac ttttcctagc cttctaatac
     4321 ctttggtttc gttggttaac tcaagaatta agcctgcttt ttcaagataa gcaagataat
     4381 caggtaataa atttctggat accccgatta aatcagcaat tttagagtaa tttggtttaa
     4441 aaggaacact ttcggaaatt atttgcaaca aatgttttag tttatcgatg gttgaaacct
     4501 tcaagtctaa atattttgga atatctacct ctaaaacagc atttacagtt cctgttaatc
     4561 gaatcataaa atcgtcgtcc ttgtaaaacg ggtaataccc atgatttaag tactttttga
     4621 agtgaatcaa aggattttcg agttttattt tcactttttg agaaagtatt tggtcaaggc
     4681 tatatgcttc cgagtcttgc cctgtttcga atatcaaata ttcgcgaaat gataatccat
     4741 ttaaatgata ggggacaaca cgcctgctta aatcaccaaa acctttataa atatcgagaa
     4801 tagaactacc gctaaacaca atttgcaaat cgggaaaact atcgtaaata ttttttattt
     4861 cttgtgacca ggtttcatac ttatgtacct catcaataaa taaatggcgg ccgccatacc
     4921 tataaaagta accagccaat tcgaacaatg tatggtttga aaaataaata ttatcgagcg
     4981 aaacgtaaag actttcatct attttgtttt caatcttaat tttttgtagc attagtgtag
     5041 tttttccaac tcctcttggc cctgtaatgg ctataagtct ggcattccag tt
//
LOCUS       DAOSAL010000121         5081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_10336_length_5081_cov_50.658774, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000121 DAOSAL010000000
VERSION     DAOSAL010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 5081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5081
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10336_length_5081_cov_50.658774"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..638
                     /locus_tag="PK049_11120"
     CDS             195..638
                     /locus_tag="PK049_11120"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOK62152.1"
                     /translation="MNYDELTKLLSEKGLKVTPQRVAVLEALYEIMNHPSAEEVRQKL
                     HSRFPSIAIGTIYNILDTFCEHGIIRKVKTDSDFVRYDVDMEKHHHIYCESCNHIENY
                     HDPELDELLKAYFDRKQIPHFQIKEFSLQIIGNYKATNYKPNQNK"
     gene            642..1154
                     /locus_tag="PK049_11125"
     CDS             642..1154
                     /locus_tag="PK049_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HOK62153.1"
                     /translation="MGKEGIRIASVDVEKLLEMLNAALAEEWLAYYQYWIGAKVMEGP
                     MRSEIEPELLLHASQELNHAVMVADRIIQLGGTPLLHPNDWFKHAGCSYDAPVDPYIE
                     AILEQNLNGERCAIRRYNEIATFTNGKDHMTHQMATQILAEEIEHENDIEDWITDINR
                     MKEEIKKFKL"
     gene            1184..1894
                     /locus_tag="PK049_11130"
     CDS             1184..1894
                     /locus_tag="PK049_11130"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOK62154.1"
                     /translation="MEENQVIAMPRIGDPAPEFKAMTTQGEINFPHDYKGSWVILFSH
                     PADFTPVCTSEFMTFASMEQKFNEANCKLVGLSVDGLYSHIAWLRTIKEKIEYKGMKN
                     VEVTFPLIEDITMDVAKKYGMIQPGESNTKAVRAVFFIDPKGIIRTIIYYPLSLGRNF
                     EELYRVLIALQTADEFGIATPADWQPGDDVIVPPAGSCGTAKDRMEGKDPNVKCFDWF
                     FCTKKLDKDTVMKKILKK"
     gene            complement(1948..2145)
                     /locus_tag="PK049_11135"
     CDS             complement(1948..2145)
                     /locus_tag="PK049_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK62155.1"
                     /translation="MNRCKCIKVPESNNGQGKFKLNAYYEFDYIPPIKDNASYYRVFS
                     LEDNISENFSIKAFNEHFRKY"
     gene            complement(2185..3039)
                     /gene="speB"
                     /locus_tag="PK049_11140"
     CDS             complement(2185..3039)
                     /gene="speB"
                     /locus_tag="PK049_11140"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HOK62156.1"
                     /translation="MIHYGDLPKPYCEFETSKVAIVPVPYDGTSTWIKGADKGPKALL
                     DASANMEIYDIETDTEVYKIGIYTDKPITEDSSPEAMVEAVKERTLSLIDKGKFTVII
                     GGEHSVSIGTIQAHAQKYKNLSVLQIDAHTDLRDSYEGSKNNHACVMARAKELCPIVQ
                     VGIRSMDSGEKANLDPNRVFWAHKIVNCDDWMDKAIDLLTDNVYLTIDLDGFDPSILP
                     STGTPEPGGLLWYPTLKFLRKVIEKRNLVGFDIVELCPNTDDKASDFLAAKLLYKILT
                     YKFLNQLK"
     gene            complement(3421..3912)
                     /locus_tag="PK049_11145"
     CDS             complement(3421..3912)
                     /locus_tag="PK049_11145"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HOK62157.1"
                     /translation="MKRIGYIMMVVLLLVSTMGMAQGPRFRGGDGNTMQTVGKGYCYL
                     NLTPEQEQKINDLRVKHLKEITPLRNELSEKQAHLRTLESADKPDMAAINKTIDEITS
                     IKAKLMKARVAHRAEVSQLLTDEQRVLFNARGQKGKGMRGHRGNMRGGYGMGMGLGPC
                     MGN"
     gene            complement(3944..4492)
                     /locus_tag="PK049_11150"
     CDS             complement(3944..4492)
                     /locus_tag="PK049_11150"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HOK62158.1"
                     /translation="MNYFTKTRVLVAIIVVLGVTLLATIGTMVYGYYKQKQFADNIAR
                     NPQERMEQQANFLRDRLNLTPSQEDIFRKSRDEFHEKTVDINRKLQRVSIEIIIELSS
                     PEPDLKLIDSLVEQYGELHKAEKKVMVDHLLEIKSACTPEQFQKFQRIVEHSQRRMMQ
                     RHRQRFQNERMRRGDSIPPRIQ"
     gene            complement(4496..4945)
                     /locus_tag="PK049_11155"
     CDS             complement(4496..4945)
                     /locus_tag="PK049_11155"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOK62159.1"
                     /translation="MRCKEINSLMEGYLKGQLDGNTLLRVEEHLGACTKCQHNFVIHK
                     SLKELANEETSEYTDNPFFASRVISRIEAHKISSHIDFRTRLIGYAAVAAALAIGLFI
                     GNQLGTLGSKVISTNSNAEMLAEEYIPSGTETIYEYDFEELQNLNEK"
BASE COUNT         1399 a         1041 c         1075 g         1566 t
ORIGIN      
        1 tgtgtgtcgc ttaaatctat ttctaacctg tagacttatt tcaggactag acatcatatt
       61 ctgcctattc cgactcattc ctctaaaagt taattcatgg cacttgggtt aaagattttc
      121 aaaaatatta ttgacttgcc taaacctttt ttgtaaattt atgttctact aaacaagaat
      181 aattcctata taggatgaat tacgatgaac taaccaagtt gctttccgaa aagggattaa
      241 aggtaacccc gcaaagggtt gccgtgcttg aggctttgta tgagattatg aatcacccct
      301 ctgctgagga ggtaaggcaa aaattgcata gccgttttcc cagcatagcc ataggaacca
      361 tatacaatat cctggataca ttctgcgaac acggcattat tagaaaggta aaaaccgata
      421 gcgattttgt taggtacgat gttgatatgg aaaagcatca ccatatatat tgtgaaagtt
      481 gcaaccatat tgaaaactat cacgatcctg agcttgatga gctcctaaag gcatactttg
      541 atagaaaaca aattccacat ttccagatca aagagtttag cctacaaatc attggaaatt
      601 ataaagcaac taactacaaa ccaaaccaaa acaaataaac tatgggaaaa gaaggaatta
      661 gaatagcatc ggttgatgta gaaaaattac ttgagatgct taacgcagca ctagccgagg
      721 aatggcttgc ttactaccag tactggattg gtgcaaaggt tatggaaggc cctatgcgaa
      781 gcgaaataga acctgaattg ttactacatg ccagccagga actcaaccat gccgttatgg
      841 tagccgatcg tatcatacag ctaggtggca caccattgct acatcccaac gattggttta
      901 agcatgccgg ttgcagttac gatgctcccg ttgaccctta cattgaagcc attctggaac
      961 agaacctgaa cggtgagcgt tgtgctattc gaaggtacaa cgagatagcc acctttacta
     1021 atggtaagga tcacatgacc caccaaatgg ctacgcagat acttgccgag gaaattgaac
     1081 atgagaatga tattgaggat tggattaccg atatcaatcg catgaaggag gagattaaga
     1141 aatttaagct gtaataattt gtcaaacctt aaaataaaca aaaatggaag aaaatcaagt
     1201 aattgcaatg ccccgcattg gcgacccagc gcctgagttc aaggccatga caacccaggg
     1261 tgaaattaac tttccgcatg attacaaggg aagctgggta atcctattca gccatccggc
     1321 cgatttcacc ccagtatgca catcggagtt catgaccttt gcctccatgg agcagaaatt
     1381 taacgaggct aactgtaagc ttgtggggct ttcggttgat ggtttatata gccacattgc
     1441 atggctgcgt accatcaagg agaagattga atacaagggt atgaaaaacg tagaggtaac
     1501 attcccgcta attgaggata ttaccatgga tgttgccaag aaatacggta tgattcaacc
     1561 cggcgagagc aacaccaagg cagtgcgtgc ggtattcttt attgatccca agggtattat
     1621 ccgaaccatc atctactacc cacttagctt gggccgtaac tttgaggagc tataccgcgt
     1681 actcatcgct ttacaaactg ccgatgagtt tggaattgct acccctgccg attggcaacc
     1741 tggtgatgat gtaattgttc ctcctgccgg ttcctgtggc actgctaagg acaggatgga
     1801 aggtaaagac cccaatgtga agtgcttcga ttggttcttc tgcactaaaa agttggacaa
     1861 ggatacggtg atgaagaaaa tccttaagaa atagctaggg aatatacttt aaacgaaaaa
     1921 cggctcaagg atttgagccg ttttttttta gtattttctg aagtgttcgt taaacgcttt
     1981 gatgctaaaa ttttcactaa tgttatcctc aaggctaaac accctgtaat acgaggcatt
     2041 atccttaatg ggcgggatgt aatcgaactc gtagtaggca ttgagtttaa atttaccctg
     2101 cccattgttc gattctggaa ctttaataca cttacagcgg ttcataggtt aggtttttta
     2161 agtttaggtt gatatttgct ttaactactt aagctggttc agaaacttgt aggttagtat
     2221 tttgtaaaga agtttggccg ccagaaaatc cgatgcttta tcgtcggtgt tgggacatag
     2281 ctcaacgatg tcgaacccta ccaggttgcg tttttcaata acctttctta agaacttgag
     2341 ggtagggtac cacaaaagcc cgccgggctc aggagttcct gtacttggca atatggaggg
     2401 gtcaaaaccg tcgaggtcga tggttaggta aacattatct gttagcaaat cgatagcctt
     2461 gtccatccag tcgtcgcagt tcactatttt gtgagcccag aacacccggt tggggtcaag
     2521 gttggctttt tcgcctgagt ccatactgcg aatacctacc tgaacaattg ggcaaagttc
     2581 ctttgcccga gccataacgc aagcgtgatt gtttttgctt ccctcgtagg aatcgcgcag
     2641 gtcggtatgg gcatcaattt gaagcactga aaggtttttg tatttctggg catgagcctg
     2701 tatagttcca atgcttacac tgtgttcacc acctataatg actgtaaatt tacctttatc
     2761 aatgaggcta agcgtccgtt ctttaaccgc ttcaaccatt gcttcgggtg agctatcctc
     2821 tgttattggc ttatcggtat aaatacctat cttgtaaacc tcggtatcgg tttcaatatc
     2881 gtatatttcc atattggccg aggcatcgag cagggccttg ggccctttgt cggcaccctt
     2941 aatccatgtg ctggttccat cgtagggtac tggaacaata gccacttttg aggtttcaaa
     3001 ttcacagtag ggtttaggta aatcgccgta gtgaatcata ggtatatgct ttcaaatttc
     3061 ccgcaaaagt actaaaaaaa aacaatttta gtttaggtaa tggaatctca ttaaaaatgt
     3121 gtagaaggta caaagagaag ctttgactta gctcagcatg acaaaggtac taaggatgcc
     3181 aggggattta tcattcctag caaatgcgag gaatctcttt caagtttgag gttagtagcc
     3241 ccgtacgggc ataatattat atcaagggta atccaattcg agggttaggc ttttatgctc
     3301 atgtttgctt ttttcccttc cattccctct cagggggggc aggtgtgagg tagttttcac
     3361 gaacaacaaa aaaggcaccc attatgagtg ccttttttta ggaggttaga accccattgg
     3421 ctagttgccc atgcatggtc ctaagcccat tcccattccg taaccgccgc gcatattacc
     3481 gcgatgtcct ctcatgccct tacctttttg gccacgtgcg ttaaacagga cacgttgttc
     3541 atcggttagt agttgcgaaa cctcagccct gtgggctaca cgtgctttca taagttttgc
     3601 tttgattgag gtaatctcat cgattgtttt gttaatggct gccatatcgg gtttatcagc
     3661 cgattcaagt gttctcaaat gcgcttgctt ttcgctaagc tcgttacgga gcggtgttat
     3721 ttcttttagg tgtttaaccc gaaggtcgtt aattttttgc tcctgctcag gggttaggtt
     3781 taggtagcag taaccttttc caactgtttg catggtattt ccatcgccac ctcggaatct
     3841 tggcccttga gccataccca ttgtagatac taaaagtagc actaccatca taatgtaccc
     3901 aatacgtttc attgtgttaa attttaatag ttaaacgtca gttttattga attcggggtg
     3961 gaatagaatc gcccctacgc atgcgttcgt tttgaaatct ttgacgatgt cgttgcatca
     4021 ttcggcgttg tgaatgctcc acaattcttt gaaatttttg gaactgttca ggagtgcatg
     4081 cactcttaat ctccaacagg tggtcaacca taaccttttt ttcagcctta tggagttcgc
     4141 catactgttc aaccagtgaa tcgataagtt ttaaatccgg ttcaggcgac gaaagctcaa
     4201 tgataatttc aattgaaacc ctttgaagct ttctgttaat atcaactgtc ttttcatgga
     4261 actcgtcgcg agattttctg aaaatatcct cctgactggg tgtaagattt agcctatcgc
     4321 gtaaaaaatt ggcttgctgt tccattcgtt cctgcgggtt acgggctata ttgtcagcaa
     4381 attgtttttg tttgtagtag ccgtaaacca ttgtgcctat tgtagccagt aaggttactc
     4441 ctaatactac aattattgct acaagcactc gtgttttggt aaagtagttc atgatttatt
     4501 tttcattaag attttgaagc tcttcaaagt cgtactcata aatggtttcg gttccagatg
     4561 gaatgtactc ctcagcaagc atttcggcat tggaattggt tgaaataact ttacttccca
     4621 gtgttccaag ttggtttcca atgaataacc ctattgccaa tgcggctgcc acagcagcgt
     4681 atccaattaa acgtgttctg aaatcaatat ggctagaaat tttgtgagcc tcaattctgc
     4741 taattaccct gcttgcgaag aatggattat cggtatattc tgaagtctct tcgtttgcaa
     4801 gctcttttag tgatttgtgg attacaaaat tgtgctgaca cttggtgcaa gcgcctaaat
     4861 gctcctctac tctaagcaaa gtattaccat caagttggcc tttaaggtaa ccctccatta
     4921 gactatttat ttctttgcac ctcattttta tttgtttttt tagtttgaca actttatatc
     4981 ttaaaacttg cgttaccctt tttctttttt tagggttttg ttttaaccat tatcttgcgc
     5041 aggttatttt tagccctgaa taaaagcgac tcaaccgacg a
//
LOCUS       DAOSAL010000122         5007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_10550_length_5007_cov_51.667407, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000122 DAOSAL010000000
VERSION     DAOSAL010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 5007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5007
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10550_length_5007_cov_51.667407"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="PK049_11160"
     CDS             <1..254
                     /locus_tag="PK049_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016270588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6175 family protein"
                     /protein_id="HOK62160.1"
                     /translation="IIEDWFADNCVEGRFNLSDATENFMKFEQVRIPMMRTDEKGRQR
                     AVDTRSFVSDLRKYLNGMGIPSKLYMRGLGEAWLILGEK"
     gene            266..1237
                     /locus_tag="PK049_11165"
     CDS             266..1237
                     /locus_tag="PK049_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK62161.1"
                     /translation="MKKLILSIAVLTGFALSLNAQNSQGKTDDMGRIALAAIVPEQAT
                     GIPQEARQMLVNKMSQIATQNGLGATAANPRFCIVPLVTVISREITPTAPPMQAVNLD
                     VTFYIVDAASQNIFSQTNISVKGVGQSEDKAYIQALKNINVKAGQFKGFIEKGKEKII
                     EYYNSQCDVVLKGAQALAGQRKYEEALFVLLSVPDVCRECFDKSMDLSIEIYKQYANY
                     KCTEYMAGAKAAWANMNADKAAEYLGKITPDMECYADAVRLTDEITQKMLADGANVWN
                     FKMKQYDDSIEKDKMMIQAGKEIAVAWAYWGAAPYFNWDWSWLYQQK"
     gene            1301..1870
                     /locus_tag="PK049_11170"
     CDS             1301..1870
                     /locus_tag="PK049_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK62162.1"
                     /translation="MRNLLMVAGIFTFAFMLASCGGSKKAADSSLGKEINVPCNDDEF
                     HTDAKYFRGTGNGISSDLSTAKSKARIDAQTNLSRSISTTMKAVADRYVNERQVGDAM
                     EFEQKFEQMTREVINQELNNVEVVCTKTFQKEGRYQVFNALQVPKDQVLNNIKDRISK
                     DQKLQLDYDKMKFEQVFNEEMEKMAKERP"
     gene            1974..3263
                     /locus_tag="PK049_11175"
     CDS             1974..3263
                     /locus_tag="PK049_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK62163.1"
                     /translation="MKKILLLALSVVGVITLTFAQDKLLEKSGKQPKWVNGLEKDFII
                     VTGTGPTAQDAQQNALSNVKAQIVSSVAENVKASSEVRKEEASFNNNVNAFLEKFATT
                     TTTQSGKVPFLQGISISKVSEFYWEKRQRADKSVYFIYHIKYPFPETELKMLVLDYKM
                     RDRELTRQMEDLLEQVDKVESVEQIEKNIAELGSLKDYFMDGRVDQCNLGITRYKSLL
                     SSIELVELESNLGELKYYLRLNGRAISTAKKPTTKSECARITSVINNSDNVVIKYNYE
                     NCYEDPENNILVKYMFGGVTVQKPFYFDVAANKASIFLSDPIHLNAIAKDDANVTGII
                     VDMTIRSKYDSPFVVEKVVLEWKGFPPVVIENIGQSFKGKGNHTLKVNSDQSLKIAET
                     SSSGKTLSLLSGYIHYRDASGELKTYRIYNHSYTTDW"
     gene            complement(3313..3693)
                     /locus_tag="PK049_11180"
     CDS             complement(3313..3693)
                     /locus_tag="PK049_11180"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HOK62164.1"
                     /translation="MEPLKVNGTSQYPTITLDKDAGVFEFKGNSLPEDAKSFFEPIIT
                     WIEEYAKSPNPETVVRFKMIYYNTPSSKMLFQVLKRFERIAQDGHKISIVWMYNEDDI
                     DIKDAGKELSNHIKIPFKLESYKE"
     gene            complement(3822..4712)
                     /locus_tag="PK049_11185"
     CDS             complement(3822..4712)
                     /locus_tag="PK049_11185"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOK62165.1"
                     /translation="MIILETPTNWPDYELIDSGDGEKLERFGSQILARPEPQAIWRKA
                     LSQAEWNSLANAYFVKKKGNRESDNEWGEWNKKGNCKEQWKIGYSYKQMKLKFRLGLT
                     SFKHVGIFPEQATNWDFIYDTVQHLSAEKPSVLNLFAYTGGASLAARAAGADVTHVDS
                     VKQTITWSRENMELSKLDGIRWMVDDAVKFVAREVRRAKTYNGIILDPPAYGRGPEGE
                     KWILETNLCPLLENCKNLLTHKNSFLILNLYSMGYSPIIAESLVKSIFKVDKPEIGEL
                     YVVDRAGRKLPLGVFLRFLK"
     gene            complement(4735..>5007)
                     /locus_tag="PK049_11190"
     CDS             complement(4735..>5007)
                     /locus_tag="PK049_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK62166.1"
                     /translation="YKANVANRASTNFLLKHGAQSIEPAFEVKRPKGEVELMVTRYCI
                     KHELGLCPSKQGAKPTGRLFLKNDHNLFPLVFDCKNCVMKVIQPNK"
BASE COUNT         1463 a         1024 c         1068 g         1452 t
ORIGIN      
        1 ccattattga ggactggttt gccgataact gcgtggaagg tcgctttaac ctttccgatg
       61 caactgaaaa cttcatgaag tttgagcagg ttcgtatccc tatgatgcgt actgacgaaa
      121 aaggtcgcca aagagcagtt gacacccgct catttgttag cgatttgcgc aagtacctga
      181 acggtatggg aataccctca aagctgtaca tgcgcggttt gggtgaagct tggctaatac
      241 taggtgaaaa gtaactgata ttaaaatgaa aaagttaata ctatcaatag ctgtgctaac
      301 aggctttgct ttaagcctta acgcacaaaa cagccaaggg aaaactgacg acatgggcag
      361 aatagcactt gctgctattg ttcccgaaca ggcaaccggt ataccccagg aggctcgcca
      421 gatgttagtt aacaagatga gccagattgc cactcaaaac ggtttaggcg ctactgctgc
      481 taatccacgc ttttgtattg taccactcgt aacagtcatt agcagggaga ttactcctac
      541 agcaccccct atgcaggctg taaacctgga tgttaccttt tatattgttg atgctgctag
      601 ccaaaacatc ttcagtcaaa caaatatatc cgttaagggc gttggtcaat ccgaagataa
      661 agcatacatt caggctttaa agaacatcaa tgttaaggct ggtcagttta aggggttcat
      721 tgaaaaaggt aaggaaaaga tcattgaata ctacaactct cagtgcgatg tagtgctaaa
      781 gggagcccaa gctcttgccg gccaaagaaa gtacgaagag gctcttttcg ttctgctatc
      841 agttcccgat gtttgccgcg aatgctttga caagagcatg gatttgtcaa tcgagattta
      901 taagcagtac gccaactaca aatgtactga gtacatggca ggtgccaagg ctgcttgggc
      961 taacatgaat gccgataagg cagctgagta tctaggcaaa attactcccg atatggagtg
     1021 ctatgccgat gcagtacgtt taaccgacga aatcacccag aaaatgctgg ccgatggtgc
     1081 taacgtatgg aactttaaaa tgaaacaata cgacgattcc attgaaaaag ataagatgat
     1141 gatacaggct ggcaaggaaa ttgcagtagc ttgggcatac tggggtgctg ctccttactt
     1201 caactgggat tggagttggt tgtaccagca gaaataaacc gataattaat ttaataacaa
     1261 cttgatttta agtttaacca aaaaaagaat gactatgaaa atgagaaatt tattaatggt
     1321 tgccggcatt tttacctttg cttttatgct ggctagttgc ggtggttcaa agaaagctgc
     1381 agacagctct ttaggtaagg aaattaatgt tccttgcaac gatgatgaat tccacaccga
     1441 tgctaagtac ttccgtggaa caggtaacgg tatcagcagc gatttaagta ctgcaaagag
     1501 caaagctcgt atcgatgcac aaaccaacct ttcacgtagc attagcacta ccatgaaggc
     1561 tgttgctgac cgttatgtaa acgaacgtca ggttggcgat gccatggagt ttgaacagaa
     1621 gtttgagcaa atgacccgtg aggttatcaa ccaggagttg aataacgttg aagtggtttg
     1681 taccaaaact ttccagaaag aaggtcgtta ccaagtattc aatgctcttc aggttccaaa
     1741 ggatcaggtt ctaaacaaca ttaaggaccg tatttcaaag gatcagaaac tacagctcga
     1801 ctacgataag atgaaattcg agcaagtatt caacgaggaa atggaaaaga tggctaagga
     1861 acgcccataa tttccctatt catttcatac agcagcccca accattgggg ttgctgtttt
     1921 tgtaaaatat ttattacttg ccaatcccct tttggggtta aaataatgtg agtatgaaaa
     1981 agatattact tcttgcttta tccgttgtgg gggttataac cctaaccttt gcccaggata
     2041 aactactgga gaaaagcgga aagcaaccca aatgggtgaa tggtttggaa aaggatttca
     2101 tcattgtaac gggtacaggc cctacagcac aggatgccca gcagaacgcc ctttcaaacg
     2161 ttaaagccca aatagtatcg tccgtggctg agaacgttaa agcaagctcc gaggtacgta
     2221 aggaggaggc cagcttcaac aataatgtta atgcattcct tgaaaagttt gccaccacta
     2281 ccaccactca atcaggtaaa gtaccttttc tgcaagggat ttctatctcc aaggttagtg
     2341 agttttactg ggaaaaacgg caacgtgccg acaagtcggt ttatttcata taccatataa
     2401 aatatccttt cccggaaaca gagctgaaaa tgctggtgct tgattacaag atgcgcgacc
     2461 gcgagctaac ccgccagatg gaagatttgc ttgagcaggt tgataaggta gaatctgttg
     2521 aacagattga gaaaaacatt gctgagcttg gctcgcttaa ggattacttt atggatggaa
     2581 gggttgacca atgcaatctt ggtattaccc ggtacaaatc attacttagc tccattgaac
     2641 ttgttgaact ggaaagcaac cttggtgagc ttaagtacta cttacgtttg aatgggcgag
     2701 ccatttccac ggctaaaaag cccacaacca aatcggaatg tgccagaatt acttcggtta
     2761 tcaacaattc cgataatgtg gttattaaat ataactatga gaattgctac gaggatccgg
     2821 aaaataacat tctggtaaaa tacatgtttg gaggtgtaac cgttcaaaaa cctttctatt
     2881 ttgatgttgc agccaataag gcatcaattt tcttatcgga ccccatacat ttgaatgcca
     2941 ttgcaaagga tgatgcaaat gttactggaa taatagttga tatgaccatt cgctccaagt
     3001 acgattctcc ttttgtggtt gaaaaggttg tgctggaatg gaagggtttc ccacctgtag
     3061 ttattgagaa tattgggcag tcgttcaaag ggaagggcaa tcataccttg aaagtaaact
     3121 ctgaccagtc ccttaagatt gctgaaacat cctcgtcagg taaaacccta tcgttgctct
     3181 caggttatat ccactaccgc gatgcctcag gcgaactaaa gacttacagg atttacaacc
     3241 acagctacac caccgattgg taataggttg ttagaatatt gttcaataaa aaaaggcctt
     3301 tacggccttt ttttattcct tatatgattc caacttgaaa ggtatcttga tatgatttga
     3361 gagttctttc ccagcatctt ttatatcgat gtcatcctca ttatacatcc aaactatact
     3421 tatcttgtgg ccatcctgag caattctttc aaatcgcttc agtacctgaa aaagcatttt
     3481 tgagctgggg gtattgtagt aaatcatttt aaatcgtacc actgtttcag gatttgggct
     3541 ctttgcatac tcttcaatcc aagtaattat tggttcgaaa aatgattttg catcctcagg
     3601 taaagagttt cctttaaact caaagacacc tgcatcctta tcgagagtaa tggttggata
     3661 ttggctggta ccgttaacct ttaatggttc catacagcat tggttttata aatttcaata
     3721 acttcccata aaacaaatat acggaaaatc aatgcaaaag taacattata ttgatatatt
     3781 ttacctgttt tatggcaggc tttttatttt tgttgataaa attattttaa aaaccgcaaa
     3841 aacacaccca gcggtaattt tcgtccagct ctgtcaacta cgtaaagttc tccaatttct
     3901 ggtttgtcaa ctttaaaaat gcttttaacc agactttcgg ctattatggg cgagtagccc
     3961 attgagtaaa ggtttagtat caggaaggag tttttatggg ttaatagatt tttgcagttt
     4021 tccagaagcg gacatagatt tgtttcaagt atccattttt caccttcggg ccccctacca
     4081 taagctggag ggtcaagtat aattccattg taggttttgg cgcgccgtac ctcgcgtgct
     4141 acaaatttta cggcatcatc taccatccac ctaattccat cgagcttgct aagctccata
     4201 ttttcgcgtg accaggtaat ggtttgctta accgaatcga catgggttac atcggccccg
     4261 gctgctctag cagccaacga tgcacctccg gtgtatgcaa acagatttaa aaccgatggt
     4321 ttttctgctg atagatgctg aacagtgtcg taaataaaat cccagtttgt tgcttgttca
     4381 gggaatatgc caacatgctt gaacgatgta aggcccaaac ggaactttag cttcatttgt
     4441 ttgtagctgt aaccaatttt ccattgctcc ttacagtttc ccttcttatt ccactcgccc
     4501 cattcgttat cgctctcgcg gttacccttt ttctttacaa agtatgcatt ggctaagcta
     4561 ttccattcgg cttgtgaaag tgcttttcgc caaatggctt gaggctcggg acgagccaga
     4621 atttggctgc cgaaccgttc tagcttttcg ccatcgccgc tgtcgattag ctcgtaatca
     4681 ggccagttag tgggtgtttc aagtataatc atcaatctaa tttttaaaaa acttttactt
     4741 attgggttga atcaccttca tcacacagtt cttacagtca aaaaccagag gaaaaaggtt
     4801 atggtcattt ttgagaaaaa gtcgtccggt tggtttggcg ccttgcttgc ttgggcataa
     4861 gcccagctcg tgtttgatac agtatcgggt aaccattagc tccacctcgc ccttggggcg
     4921 cttaacctca aatgcaggtt caatgctttg ggctccatgt tttagcagga aattggtgct
     4981 tgccctgttg gcaacattgg ctttata
//
LOCUS       DAOSAL010000123         4917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_10826_length_4917_cov_54.064171, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000123 DAOSAL010000000
VERSION     DAOSAL010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 4917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4917
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10826_length_4917_cov_54.064171"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            299..763
                     /gene="nuoE"
                     /locus_tag="PK049_11195"
     CDS             299..763
                     /gene="nuoE"
                     /locus_tag="PK049_11195"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOK62167.1"
                     /translation="MTDTKELVKNLADKYGRSRESLIPILQGVVQEERYISSEAMTEI
                     ARELDMSAAKVYGTATFYSFLDTQPRGKYVIRVCRTISCSMKGKNLIIQELEDMLKIK
                     VGETTSNRKFSLLETNCLGWCHKGPAMLINDEAYTELTPEKVREIINEYMKK"
     gene            781..1944
                     /locus_tag="PK049_11200"
     CDS             781..1944
                     /locus_tag="PK049_11200"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOK62168.1"
                     /translation="MSNNFLRRVDLIIDKDCDFKQVLQSAYKRSNDEIINELLESGLK
                     GRGGAGFPTALKWKFTSEQSDPEKYVVCNADEGEPGTFKDREILFQVPLKVFGGMAIC
                     AKVIGAKEGYIYLRGEYRFLLKELENQLEIFHSILDDLKIPFRVKIVMGSGAYICGEE
                     SALFESMEGDRGEPRNKPPYPTVSGFRKKPTVINNVETLVYSFMIFRYGARKFKELGT
                     ADSRGSKVFSISGDTPKPGIYELELGMTVDQFVQEFGDGDTKAVQVGGASGFCVPRKK
                     FKDTIIGFEGVPTGGSMMLFNSSRSMYNVLHNYLEFFEEESCGQCTPCRVGCQQLKLG
                     IEAVKRGEKHANYLDQLQKLAQTMKITAKCGLGQSVANSFFSIVENFREEMIY"
     gene            1964..3757
                     /locus_tag="PK049_11205"
     CDS             1964..3757
                     /locus_tag="PK049_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOK62169.1"
                     /translation="MMAKQINLTIDGMPITVDEGTTILQAAKKLNIHIPTLCYHDDLC
                     VAGNCRVCVVEMKGSRTLPASCAMPVAEGMEINTNTIKVRTARKHIIDLLLSEHNAKC
                     TTCYKNGNCELQQLASEYKISEPMFIDLVPHKNYQMDAYSPSIIKDDSKCIRCQRCVR
                     TCEELQHVSALGVAYKGDKMKISTFFENPMFEVVCTNCGQCVNRCPTGALVERNYVDE
                     VWQAIFDPDKHVVVQTAPAVRVALGEALGLKPGEIVTGKMVTALKRLGFDSVLDTDFS
                     ADLTIMEEGFELLGRLKKALVDKDPNVKLPMATSCSPGWIKFIEHTYPEYLNNLSTCK
                     SPQQMFGALAKTYYAQKRNIDPAKIVSVSIMPCTAKKFEADRPEMRSSGFKDVDYVLT
                     TRELAIMIKQAGIEFSSLPESHYDSVMGASTGAAVIFGATGGVMEAALRTAYEVVTGR
                     EVPFNGLNITPVRGMEGVREAAIKIEGTKPEWKFLEGAELKTVVAHGLTNAKKVMDAV
                     RDGKGSWHFIEIMACPGGCIGGGGQPIPTNEEIRRKRAEAIYREDAGMPIRKSHENPE
                     VVAIYKEFLGEPNSHKSHELLHTHYKARQRY"
     gene            complement(4157..>4917)
                     /locus_tag="PK049_11210"
     CDS             complement(4157..>4917)
                     /locus_tag="PK049_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK62170.1"
                     /translation="LKMKKFKFYILVFICMVLQIKTFAQNEINLDNLYTTLNISLDKV
                     VKELVIVKLFPTDSSSSIIVIPVKNEDEIDDLTADMHLLKINHKTGKILCHNLFDNIL
                     LNDAIRLYAIQIDTMLYQIKENENAFAIKLHYTNDSRSAGYIGEELLLFEERDNSFIL
                     ILRLDNINENIWYGGGDCENTEIYNKKSTFIIDTMSSKNGYFNIIEDIYFEHYYLDKD
                     CEDSKKKNIKNLRNVYEFIDNSYICIGERKTGFK"
BASE COUNT         1397 a         1023 c         1032 g         1465 t
ORIGIN      
        1 tatcctttaa cctttatcct ttaaccttta acctttaacc tttaaccttt atcctttatc
       61 cttcagtaac ccagtgtcat acaacccaaa gtttcaatcc gtaactttct tactttgaat
      121 ggttctaaat tttagctttt tcagtcgttt cagtgaaaaa aatgacaaaa atcaggtttg
      181 tgatccattt aactatatat atttagggaa gtaaatcaat gttttagaaa caatattgat
      241 gttattaaag taacagtgtt aacagaataa caaaaagcaa tttttaaatt ttaaggttat
      301 gacggatacc aaggaattag tgaaaaatct tgccgacaag tacggtcggt caagggaaag
      361 cttgataccc atcctgcagg gtgtggttca ggaggagcgc tacatttcaa gtgaggcaat
      421 gactgaaatt gcccgtgaac tcgacatgtc ggccgcaaag gtttatggaa cagccacctt
      481 ttactccttc ctcgatactc agccccgagg aaagtatgta attagggtgt gccgtaccat
      541 ttcctgctcc atgaaaggga aaaaccttat aattcaggaa cttgaggata tgctaaagat
      601 taaggttggt gaaaccacct cgaacagaaa gttctcgttg ctggaaacca actgcttggg
      661 ttggtgccat aagggacccg ctatgctcat taacgacgag gcttacactg aacttacccc
      721 agagaaggtt cgtgagatta ttaatgaata catgaaaaag taaaaatagg agattcgatt
      781 atgtcaaata acttcttacg tcgcgtcgac ctgattatcg ataaagattg cgattttaaa
      841 caggtactac aatcagctta caaacgcagc aacgacgaga tcattaacga actccttgaa
      901 tcaggtctca agggtagggg tggcgccggt ttcccaaccg ccctgaaatg gaagtttacc
      961 agcgagcaat ccgaccccga aaagtatgtg gtttgtaacg ccgacgaggg cgaacccggt
     1021 actttcaagg atcgcgaaat ccttttccag gttcccttaa aggtttttgg cggtatggcc
     1081 atttgtgcca aggtgattgg cgctaaagag ggatacatct acctcagggg cgagtatcgt
     1141 ttcctgctta aggaacttga aaatcagctt gagattttcc actccattct cgatgacctt
     1201 aaaatacctt tccgtgtaaa aattgtaatg ggaagtggcg cctacatttg tggtgaggaa
     1261 agcgcattgt ttgaatcgat ggaaggcgac cgcggtgaac cccgcaataa gcctccttac
     1321 ccaaccgtat ctgggttccg aaagaaacct accgtaatca acaacgtgga aactttggtt
     1381 tactcgttca tgattttccg ttacggtgcc cgcaagttca aagaacttgg aaccgccgat
     1441 tcacgcggtt caaaggtttt ctcaatttcc ggcgataccc caaaacccgg catttatgag
     1501 cttgaactgg gtatgactgt tgatcaattc gttcaggagt ttggcgatgg tgataccaag
     1561 gctgttcagg ttggcggtgc atcgggattc tgcgtacccc gcaagaaatt caaggatacc
     1621 attattggtt tcgagggtgt acctactggt ggctccatga tgcttttcaa tagctcccgc
     1681 tccatgtaca atgtgcttca taactacctc gaattctttg aggaagaatc gtgcggacaa
     1741 tgtactcctt gccgcgtggg ctgccagcag cttaagctgg gcattgaggc cgttaaaagg
     1801 ggcgaaaaac atgccaatta ccttgaccaa ctccagaaac tggctcaaac catgaagata
     1861 accgcaaaat gcggcttagg ccaaagcgtg gctaactcct tcttctcaat cgttgagaac
     1921 tttagggaag aaatgattta ctaattttat ctaaatcgat gcaattatgg caaaacagat
     1981 aaatcttaca atagatggca tgcctataac cgtggacgaa ggaacaacca ttctgcaggc
     2041 tgccaaaaaa ctgaatattc acatacccac gctctgttac cacgatgacc tttgcgttgc
     2101 cggtaactgc cgtgtttgcg tggttgaaat gaaaggatca cgtactcttc ctgcttcatg
     2161 tgcaatgcct gttgctgagg gtatggagat caacaccaac accattaagg ttcgtactgc
     2221 ccgtaagcat attatcgacc ttttactctc ggagcacaac gcaaagtgta ccacttgtta
     2281 taagaacggg aactgcgagt tgcagcaact tgcctccgag tacaagattt ccgaacccat
     2341 gttcatcgat ttggtacccc ataagaacta ccaaatggat gcttattccc cttcaatcat
     2401 taaggacgat agcaaatgta ttcgctgcca acgctgcgtt cgcacctgcg aggaacttca
     2461 gcatgtaagc gcattgggcg ttgcatacaa gggcgacaag atgaagatat ccactttctt
     2521 cgaaaatccc atgttcgagg tggtttgtac caactgcggg cagtgcgtta accgctgtcc
     2581 aaccggcgcc cttgttgagc gtaactacgt cgacgaggtt tggcaagcca tttttgaccc
     2641 cgataaacac gttgttgttc aaaccgctcc tgctgttagg gtagcccttg gtgaggcctt
     2701 gggattaaag cccggcgaaa ttgttaccgg taaaatggtt actgcgctta agcgcctggg
     2761 cttcgattcg gttcttgata ccgatttctc tgccgacctt accatcatgg aggagggctt
     2821 tgagctgctt ggcaggttaa agaaggcttt ggttgataaa gaccccaacg taaaactgcc
     2881 tatggcaacc agctgctcgc cgggttggat taagttcatt gagcatacct accccgagta
     2941 cctcaataac ctttccactt gtaagtcacc gcaacaaatg tttggcgcat tggctaagac
     3001 ctactacgct caaaagcgta acatcgaccc cgctaagatt gtatcagtgt ctataatgcc
     3061 ctgtacagcc aagaagtttg aggccgacag gcctgagatg cgctccagcg gcttcaagga
     3121 tgttgattac gttctcacaa cccgtgagct tgccattatg atcaagcagg caggtattga
     3181 gtttagctcg cttcccgaat cgcattacga tagcgttatg ggtgcctcaa ccggtgctgc
     3241 tgtcattttt ggcgctaccg gtggggtaat ggaagccgca ttacgtaccg catacgaggt
     3301 ggttaccggt cgcgaagttc catttaacgg attaaatatc actccggttc gtggtatgga
     3361 gggcgttcgc gaggctgcca ttaagattga aggcaccaag cccgaatgga agttccttga
     3421 aggtgcagaa ctgaaaaccg ttgtggctca cggattgacc aatgccaaaa aggttatgga
     3481 tgccgttcgc gatggcaagg gaagttggca tttcattgaa attatggcat gccctggcgg
     3541 gtgtattggt ggtggtggac aacccatccc aaccaatgag gagattcgca ggaaacgtgc
     3601 cgaagccatt taccgcgagg atgccggtat gcccatccgc aaatcgcacg agaaccccga
     3661 ggtggttgcc atctacaagg agttccttgg cgaacccaac agccataaat cgcatgagct
     3721 gctgcatacc cactataaag cacgtcaacg ctactagtct ttaactcatt tattagaaaa
     3781 ggcaggatta aaaattcctg ccttttttgt atttgctttt ctaaaatccg aactaaatac
     3841 tatttgaaga cctttgccaa acaactttat ttaatttatt ttgattttgt tcggttttag
     3901 agtttatgcg atgtgtttta aaatatttga aatgaaatat aattgagtgg ttaagctttt
     3961 actatgcgga gttaaggtca taaaaagaat agttaacacg tattgtaaac cgttattttt
     4021 ttatgctttt cagaatgtta ttttctgctg gtgtgttttg actatgaaat ttattttaag
     4081 ttcttttgtt gtcatggagg taacatccca tttaaaattg atcaaatagg aacgttgaga
     4141 tttgtgaatc caaatgttat ttaaatcctg tttttctctc tcctatacat atataagaat
     4201 tatcaatgaa ctcatagaca tttcgtaagt ttttgatatt ctttttttta ctatcttcac
     4261 aatctttgtc caagtaataa tgttcaaagt aaatgtcttc aattatattg aaataaccat
     4321 tctttgaact cattgtatct ataataaaag tgcttttttt attataaatt tctgtatttt
     4381 cacaatctcc acctccatac catatgtttt cgttgatatt atctaaacgt aaaattagta
     4441 taaatgaatt atctctttct tcaaataaaa gaagttcttc gcctatatat cctgcagaac
     4501 gggagtcatt agtgtaatgc aatttaattg caaatgcatt ttcattctct tttatttggt
     4561 aaagcatggt atctatctga attgcatata gtctaatggc atcattcaat agaatgttgt
     4621 caaatagatt atgacataat attttccctg ttttatgatt tatttttaat aaatgcatat
     4681 cagctgtcaa atcatctatt tcatcttcat ttttaacggg aattacaatt attgatgaac
     4741 tactatcagt tggaaacaat ttgacaataa caagttcttt gacaacttta tcaagcgaga
     4801 tattgagtgt tgtgtataaa ttatctaaat taatttcatt ttgtgcaaat gttttaatct
     4861 gtaaaaccat gcagataaaa actaatatgt aaaatttaaa tttcttcatt tttagtt
//
LOCUS       DAOSAL010000124         4830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_11066_length_4830_cov_42.753298, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000124 DAOSAL010000000
VERSION     DAOSAL010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 4830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4830
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11066_length_4830_cov_42.753298"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..898
                     /gene="xerD"
                     /locus_tag="PK049_11215"
     CDS             2..898
                     /gene="xerD"
                     /locus_tag="PK049_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOK62171.1"
                     /translation="MGWSNAIDDYINFLRLEKSLSENSIAAYRTDMDKLWKFAEGKGV
                     EPESIRRNDLEEFLAQLHDLGLNKRSQSRILSGVRGFFKYLLLEEVIDTDPTELIDSP
                     KIGRKLPEVLSVAEIDALEAAIDLSKPDGHRNKAIIETLYSCGLRVSELVSLRLTDLF
                     FTDGFIRVIGKGDKERLVPIGTKAINDINAYLAQRNSIDSRIDPDSRNIVFLNRWGRK
                     LTREMVFTIIKKYASLAGIKKNISPHTLRHSFATHLIEGGADLRAVQEMLGHESIQTT
                     EIYTHLDNQYLRETIMMFHPHK"
     gene            923..1102
                     /locus_tag="PK049_11220"
     CDS             923..1102
                     /locus_tag="PK049_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOK62172.1"
                     /translation="MDANEKVIQTLKSAGKPMKAGEIAEAAGVDKKEVEKAIKKLVAD
                     GKLHSPQRCFYDIKK"
     gene            complement(1147..4419)
                     /locus_tag="PK049_11225"
     CDS             complement(1147..4419)
                     /locus_tag="PK049_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOK62173.1"
                     /translation="MKKLLLWVLAIAFTITAVQGQEARLMRFPAVHNNQVVFTYAGDL
                     YTVDISGGVARKLTTHVGYEMFPHFSPDGRTIAFTGQYDGNTEVFSIPAEGGTPKRLT
                     HTATLNRDDISDRMGPNNIVMAWTPDGEKIVYRSRKQSFNDFKGQLFMVPANGGMSEE
                     LPLSTGGFCSFSPDGRKLAFNRVFREFRTWKYYKGGMADDIWIFDFNTKEVTNITSNP
                     AQDIFPMWIGDEIFFLSDRDRTMNLFVYNTKTKNIEKVTNYTDYDIKFPSFDHNTIVF
                     EKGGFLFAFDVKTRTTRKINITITDDQNYSRPSLVDASKRITNADLSPNGERVVFSAR
                     GDVYTLPSEKGITYNLTASSDVHEREATWSPDGKWIAYLSDKTGEYEVYIQPQDGSMP
                     ATQVTSGINNYIFGIEWSPDSKKILFSDRLGRLQYVDIETKAVTLVTKGKYGLVNSYS
                     WSPDSKWIAYSTSDANRFSVVYIYGIDSKTTNSVTNGWFSSYGPVFSNDGKYLLYVSD
                     REFNPIYSNTEWNHAYVDMSNIYIVMLSKDTPSPFALENNEVKPETKTEQPKDKDSAK
                     KPAKTEEKPKPEVQPVKIDFDGIENRTVELPGKAGRYYNIYCIDGKVYYNYSSSKDRQ
                     PVAMMYDLKKQKETELGESIRFTISSNGKKMLVSKNRTYAVIDLPTGKINMEKTIDLS
                     DMKVWVDYHKEWKQIFDESWRQMRDFFYVENMHGVDWKAIHDKYAVLVPYVNHRNDLT
                     YIIGEMIGELNVGHAYVNSGDKPEPERIKTGLLGAKLSKHSSGYFRVDKILEGANWSD
                     ALRSPLAEIGVNVKEGDYIISVDGVDLKNIPDIYSTLINKADKTVALVVNSSPQANGG
                     RKVLVKPIADESELYYYTWVQNNIRKVNEATNGEVGYIHIPDMGVAGLNEFVKYFYPQ
                     LTKKALIIDDRGNGGGNVSPMILERLSRVVYRMTMRRGFDQPSTIPSETHYGPKVVLI
                     DRYSASDGDLFPYGFKKLGLGPLIGVRSWGGVVGISGSLPFVDGGDLRKPEFTSFSSD
                     TGEWIIEGHGVDPDIEIDNDPYREYMGQDDQLNKAIEQIKVMLKDYKPLPQIPQAPDR
                     SK"
     gene            4672..4754
                     /locus_tag="PK049_11230"
     tRNA            4672..4754
                     /locus_tag="PK049_11230"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4706..4708,aa:Tyr,seq:gta)
     gene            4760..4830
                     /locus_tag="PK049_11235"
     tRNA            4760..4830
                     /locus_tag="PK049_11235"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4792..4794,aa:Thr,seq:ggt)
BASE COUNT         1236 a         1085 c         1149 g         1360 t
ORIGIN      
        1 aatgggatgg agcaacgcaa tagacgatta cattaacttt ctgcgacttg agaaatcgct
       61 ttccgagaac tccattgcag cttaccgcac cgatatggat aagctctgga agtttgccga
      121 ggggaaaggc gttgagcctg aatcgatacg gcgtaacgat ttggaggagt tcctggctca
      181 gcttcacgat ttaggcttga ataagcgttc gcaatccagg attctttcag gagttagagg
      241 cttttttaag tacctgctac ttgaggaggt gattgacacc gatcctaccg aactgatcga
      301 ttctcccaaa ataggccgaa aattgcccga agttctaagc gtagctgaaa tcgatgcttt
      361 agaggcggcc atcgacctta gcaaacccga tgggcacaga aacaaagcca ttattgaaac
      421 gctttacagc tgtgggcttc gtgtgtcgga gttagtgtcg cttaggctta ccgatttgtt
      481 ctttaccgat ggctttatta gggttattgg taaaggcgat aaggaaaggc ttgtgcccat
      541 tggcacaaaa gccattaacg acatcaatgc ataccttgcg cagcgtaaca gcatcgattc
      601 acgtattgat cctgattcgc gcaatatcgt tttcctaaac cgatggggcc gaaagctaac
      661 ccgcgagatg gtgttcacca ttattaaaaa gtatgcttca ctggccggga ttaaaaagaa
      721 cataagcccc catacgctca ggcattcctt tgccacccat ttaattgagg gaggagccga
      781 tttgcgcgcc gttcaggaga tgcttgggca tgaatcgatt caaaccaccg agatttatac
      841 acatctcgat aaccagtacc taagggaaac aataatgatg tttcatcccc ataaataaat
      901 gtcaaactaa aatgagttta ctatggacgc aaatgagaaa gtaattcaaa ccctaaaatc
      961 ggctggtaag ccgatgaaag caggtgagat agccgaagct gcaggtgttg ataagaaaga
     1021 ggttgaaaag gcaatcaaaa aactggtggc tgatgggaag cttcactctc cccaacgctg
     1081 tttttacgat atcaaaaagt aaataaaaaa aacgggttga aaacttcaac ccgttttttt
     1141 atttcattac ttcgacctgt caggtgcttg gggtatttgc ggtagaggct tgtaatcctt
     1201 aagcataacc ttaatctgct caatagcctt attcagctga tcatcctgcc ccatgtactc
     1261 cctgtaaggg tcgttgtcaa tttcaatatc gggatcaacg ccatgtcctt caattatcca
     1321 ctcacccgta tcggagctaa agctggtaaa ttctggttta cgcaggtcgc caccatcaac
     1381 aaatggtagt gagcctgaaa taccaacaac gccgccccaa ctcctaacac caataagtgg
     1441 cccaaggcct aactttttga aaccgtaagg gaaaagatcg ccgtcggagg cggagtaacg
     1501 gtcaatgagc acaacctttg gcccgtaatg cgtttccgaa gggattgttg atggttgatc
     1561 gaatccacgg cgcatggtca tgcggtaaac cacacggctt aggcgttcaa gtatcattgg
     1621 cgatacattt ccaccaccat tacctctgtc gtcaattatt aaagcctttt tggtgagctg
     1681 agggtagaag tatttcacaa actcattgag cccggcaaca cccatatcgg gaatatggat
     1741 gtagcccact tcgccattgg tggcttcgtt taccttgcga atattgttct gaacccaggt
     1801 atagtagtaa agttccgatt cgtcggcaat gggttttaca agcaccttgc ggcctccgtt
     1861 agcctgtggt gaactgttta caacaagagc aactgtttta tcggccttat taataagggt
     1921 agagtagata tcggggatgt ttttgaggtc aaccccatcc actgaaatta tatagtcgcc
     1981 ctccttaaca ttcacaccaa tctcggccag tggtgaacgg agcgcatcgc tccagttggc
     2041 gccttcaagt attttgtcaa ccctgaagta gccgctgctg tgcttgctca gtttagctcc
     2101 taacagcccg gttttaatgc gttcgggttc tggcttatcg ccactgttca cgtaagcgtg
     2161 accaacattc agttcgccaa tcatttcgcc aatgatatag gttaggtcgt tgcggtggtt
     2221 aacgtagggt accagtacag cgtatttatc gtgaatggct ttccaatcaa caccatgcat
     2281 gttctctaca tagaagaaat cgcgcatctg acgccagctc tcgtcgaata tttgtttcca
     2341 ctccttgtgg tagtcaaccc aaaccttcat gtcacttaaa tcaatggtct tttccatgtt
     2401 gattttcccg gttggcaggt cgattactgc ataggtgcgg tttttcgata ccagcatctt
     2461 tttcccgttc gatgaaatgg tgaacctgat ggattcgccc aactcggtct ccttttgttt
     2521 cttcaggtcg tacatcatgg ccacaggttg gcgatccttg gagctggagt agttgtagta
     2581 aaccttgcca tcaatacagt agatgttgta gtatcggcct gctttgcccg gtaactcaac
     2641 ggtgcgattc tcaatgccgt caaaatcgat tttaacaggc tgaacttcag gctttggttt
     2701 ctcctcggtt ttagctggtt ttttggctga atccttgtcc ttgggctgct ctgttttggt
     2761 ttcgggtttt acctcgttat tttcaagggc aaagggtgag ggtgtatcct tggagagcat
     2821 cactatgtat atgttgctca tgtcaacata ggcgtgattc cactccgtgt tgctgtatat
     2881 gggattaaac tccctatccg atacgtaaag caggtacttt ccatcgtttg aaaataccgg
     2941 cccatacgat gagaaccagc cattggtaac ggaattggtg gtcttactgt caatgccgta
     3001 gatgtaaacc acagagaatc ggttggcatc ggaggtagag taggcaatcc atttgctatc
     3061 gggcgaccag ctatagctgt ttaccaagcc atacttaccc ttagtaacca gggtaacggc
     3121 tttagtttct atatcaacat actgtagcct acccaagcga tcgctaaaaa gtattttttt
     3181 actatccggc gaccactcta tgccaaaaat gtagttgtta atgcccgagg ttacctgagt
     3241 ggcgggcatg ctaccgtcct ggggttggat gtaaacctca tactctcctg ttttatcgga
     3301 gaggtaggca atccatttac catcgggcga ccaagtggct tcgcgctcat ggacatcgga
     3361 tgaagcagtt aggttgtaag taataccctt ttccgatggt agagtgtaaa catcgccacg
     3421 tgcactaaaa actacccgtt cgccattggg cgagaggtcg gcattggtga tgcgcttgct
     3481 tgcatcaacc aacgaggggc gggagtagtt ctggtcgtcg gttatggtga tatttatttt
     3541 ccgggttgta cgtgttttta catcaaaggc aaataggaat ccgcccttct caaaaacaat
     3601 ggtattatgg tcgaacgagg ggaacttgat atcgtaatcg gtgtaattgg taaccttttc
     3661 gatgttcttg gtttttgtgt tgtacacaaa aaggttcatg gttctgtcgc ggtccgatag
     3721 gaagaagatt tcatcgccaa tccacatggg gaaaatgtcc tgtgcggggt tactggtgat
     3781 gttggtaacc tctttggtgt taaagtcaaa aatccagata tcatctgcca tgccaccctt
     3841 gtagtatttc catgtacgga actcacggaa aaccctgttg aaggcaagct ttctcccatc
     3901 gggcgagaat gagcaaaagc caccggtgga aaggggtagt tcctcgctca tgccgccgtt
     3961 ggcggggacc atgaatagtt gccccttaaa atcgttaaag ctctgcttgc gcgagcggta
     4021 aacaatcttt tcgccatcgg gcgtccatgc cattactatg ttgtttggtc ccattcggtc
     4081 cgagatatca tcgcggttta gggtagccgt atgggttaaa cgcttgggag tacctccctc
     4141 agcaggaatg ctgaaaacct cagtgtttcc atcgtattgc ccggtaaagg caatggttct
     4201 accatcgggc gaaaagtgag gaaacatctc atacccaaca tgggtggtta gcttgcgggc
     4261 aacgcctccg cttatgtcaa cagtgtatag gtcgccggca taggtaaaca ccacctgatt
     4321 gttatgcaca gccgggaaac gcattaagcg tgcctcctgc ccctgaactg ctgtgatggt
     4381 aaaggctata gccaataccc aaagtaacag ttttttcatg gttataggtt ttagttgtaa
     4441 agttgatttt agtgtaagtt agatttattt catgaaaaaa agtttatccc ctcagttaaa
     4501 taataagaat acacctataa aaccatgtaa tgaattacta ggtttgttga tgttagtgtt
     4561 gattttcaac cccttcgcct ttcggcgtaa attttttcaa ggccaatagc aaagggcgct
     4621 tgcgagtttt gctaaaatta ctacttttgc aacgcgattt ttgagaggga aggggagata
     4681 ccaaagtggc caactggggc agactgtaaa tctgctggct tatgccttcg taggttcgaa
     4741 tcctgctctc cccacacttg ccgatgtagc tcaggggtag agcacttcct tggtaaggaa
     4801 ggggtcacga gttcaattct cgtcatcggc
//
LOCUS       DAOSAL010000125         4815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_11121_length_4815_cov_51.224370, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000125 DAOSAL010000000
VERSION     DAOSAL010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 4815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4815
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11121_length_4815_cov_51.224370"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="PK049_11240"
     CDS             complement(<1..201)
                     /locus_tag="PK049_11240"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HOK62174.1"
                     /translation="MESKIFQVTGMSCAGCAISVETILKSQPGVLDAGVNFASSTVWV
                     NFDSTITSPESLRNAVRSIGYDL"
     gene            complement(589..876)
                     /locus_tag="PK049_11245"
     CDS             complement(589..876)
                     /locus_tag="PK049_11245"
                     /inference="COORDINATES: protein motif:HMM:NF012393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOK62175.1"
                     /translation="MKELVPVNQHVVLDLSDDNTEQVTSSGIIIPDTAKEKPSWARVV
                     AVGNIENSEIAINDLVVFKKYSGTEVEFEGKTYLLIPYADLLAKVVETESI"
     gene            complement(888..1469)
                     /locus_tag="PK049_11250"
     CDS             complement(888..1469)
                     /locus_tag="PK049_11250"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOK62176.1"
                     /translation="MREITKLTEFTEQIKGKERAYLLVFKKGTGVSDCAFSAVNEAVN
                     TTRMDNVFYVDAPTYPEIHKYYGVTSAPSLLVFEKGQLKDMVKGCHDTQFYKQLFENA
                     VFQQRVQNSTQQKSVTVYSTPTCPWCNTLKSFLRKNGIVFTDIDVSRDHEAARQMVSA
                     TGQQGVPQANIGGEWVVGFDQQKIKRLLNIQTQ"
     gene            1726..3450
                     /locus_tag="PK049_11255"
     CDS             1726..3450
                     /locus_tag="PK049_11255"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOK62177.1"
                     /translation="MVKSFFWKYSLLILFFSLSYSGFAEIPSQEPQGVDEYIKLAREY
                     ENAGNANQAVYYYDKAANAYWRANDYQNAIVYFEKALENITKVGNKNGEKLLYVNLGL
                     VYSEISDYTHAQKCFESALNISTSSGKKGDVGQILYNISTVQIEQGNYQGALSNLTKT
                     EAIAQEVGDQKLLRNVYYNYNRAYEGLKNTEKAAEYFSLYAMLTKKIQAEEIRKKELQ
                     AKAMVDSAGRVVQQVSQEKEHTEKRLVETSKELKEKEQTLKEVEELTREQQMQIELLN
                     AEMKLRDAVIQHQKLLQKVYIGLILFSLAFAAALYYAYVQKRKANRLLKEKNDEISRQ
                     RDAISRQADELRELNALKDKLFSIIAHDLRSPLFSLITMLNIAKEGHFTPENFKEILD
                     ELSVNVNHTTALLENLLTWAKTQMHGVKVNPQNFDLNQLVNSRIQLLNEAAENKEIKL
                     KNSIPDGTFVFADTDMSDIVIRNLISNAIKFCNAGDRITIWSTTCADRVTVCVEDTGR
                     GMTPDVLQKLFGTQITSTPGTKNEKGTGLGLILCKEFVQMNGGEIWAESQPDKGSKFF
                     FTLPKASV"
     gene            3563..4258
                     /locus_tag="PK049_11260"
     CDS             3563..4258
                     /locus_tag="PK049_11260"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HOK62178.1"
                     /translation="MSIALIKYLSEWGKRPSDMHKEPGPVVTISREMGCPGKQVSSTL
                     IEELNKRYRLKNELAWRWLAKEEILQIASAKLGLPKEDIDYVFEAKRKGIMQEILESM
                     STKYYKSDKHIQNTVKSIIRSEASKGHVVILGRGGVAITRDIPRSIHIHLEAPFEWRV
                     LRVEEMYRFEPKEAELYVREIDRKREEIRVYFGGKDTDYTRFDITFNAMTLSVHEIVD
                     IIIHAMEVRNLIV"
     gene            complement(4267..>4815)
                     /locus_tag="PK049_11265"
     CDS             complement(4267..>4815)
                     /locus_tag="PK049_11265"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HOK62179.1"
                     /translation="FTITLLAIGLSFDTFAVSITCGIARKHIVFLEAFRVAAVFAFFQ
                     STMPLLGWGFGLAIKSVIEPIDHWLALALLCLIGVKMIVEAFGREENNSTLDPLNPKV
                     LFTLALATSIDAFAVGIGFVAVKVNMLHAYLIIGSVTFLVAMLGMLFGKSIGSVLGKR
                     MEIIGGLILIGLGLKIVIEHVG"
BASE COUNT         1545 a          895 c         1033 g         1342 t
ORIGIN      
        1 caagtcgtaa ccaattgaac gaacagcatt gcggagactt tcaggggatg tgattgtact
       61 gtcaaagttt acccaaaccg tactgctggc aaagttaacc ccagcatcca acactcccgg
      121 ttggcttttg aggatggttt ccacgcttat tgcacagcca gcacacgaca tgcctgtaac
      181 ctgaaatatt ttgctttcca tgttagtaaa aaataaatat gtaaaagtat gtgagtaaac
      241 tctcaaaaaa tggtttactg ctattgataa aaaacaactc tcatattgaa taggtttaac
      301 cgaagtgata tgaatttata ggaatgagtc ggaatgagtc ggaatgagtc ggaatgaatc
      361 ggaatgagac ggaatgagtc ggaatgtgag gaagttagaa gaagttagaa gaagttagaa
      421 gaagttagaa gaagttagag gaagttagag gaagttagag gaagttagag gaatagtgag
      481 aaaaacgatt aattcatagt acttgggttt aaagcagagc attttaaaaa agtaaaaagc
      541 acgccaagcg gtggcgtgca aaaacttagg aaagtcagaa tacgagagtt aaattgattc
      601 agtttccaca acctttgcca gcaaatcggc gtaaggtatt aggaggtaag tttttccttc
      661 gaattcaacc tcggttcctg aatacttctt aaaaaccacc agatcgttaa tagcaatttc
      721 ggagttctcg atgttgccta cggcaactac ccttgcccac gatggttttt ccttggctgt
      781 atcgggaata atgatacccg atgatgtaac ctgttcagtg ttatcgtcgc tcaggtcgag
      841 cacaacatgc tggttaactg gcactaattc tttcatatcg aactaattta ttgggtttga
      901 atattaagta gtcgtttaat tttttgctgg tcgaagccta ccacccattc accgccaatg
      961 ttggcttgag gaacaccctg ttggcctgtt gctgaaacca tttggcgagc agcctcatgg
     1021 tcgcgcgaca catcgatgtc agtaaaaact ataccattct tacggagaaa acttttaagg
     1081 gtgttgcacc atgggcaggt tggagtagaa tagaccgtta cgctcttttg ctgagttgag
     1141 ttttggaccc tttgctggaa gacggcattc tcaaaaagct gtttgtagaa ctgtgtgtcg
     1201 tgacagccct ttaccatatc tttaagttga cccttttcaa aaaccaacag cgatggagcg
     1261 ctggttaccc catagtactt gtgaatttca gggtaggttg gggcatcaac ataaaataca
     1321 ttatccattc ttgtagtgtt aacagcttcg ttaactgcac tgaatgcaca gtcgctaacc
     1381 ccagttccct ttttaaatac taagaggtaa gccctttcct ttcccttaat ttgttcagtg
     1441 aattctgtta gtttggttat ctcacgcata actatcaatt tttaaaagtc ataatcaaat
     1501 ttcaaaccaa acatatataa atgtaaatat gtcaatataa agtatgacag gtttgctgat
     1561 tttgaaaatt tgtcattatt ttgaaactta aatttacaac acaaaaaaag ccatttgcct
     1621 acacgtaagt tccattcaca gaaaaaatgg tgtaaaacgt atcgataaaa gattagtcca
     1681 cgtatgattc aataaaaaat tgcagttggt ttaaaaggat aaactatggt taagtcattt
     1741 ttctggaaat attcactact aatattattt ttctctttgt cctattccgg ttttgcggaa
     1801 ataccttcac aggaacctca aggtgttgat gagtatatta agctggccag ggaatacgaa
     1861 aatgccggga atgcaaatca agctgtttac tactacgata aggcagcaaa tgcctactgg
     1921 agagcaaatg attaccaaaa tgcaatagtt tattttgaaa aagcccttga gaatattact
     1981 aaagttggga ataagaatgg cgaaaagctt ttatatgtaa atcttggttt agtttattct
     2041 gaaatttcag actataccca tgctcaaaaa tgttttgaga gtgcgcttaa catttccact
     2101 tcaagcggga aaaaagggga cgttggtcaa atactttata atatttcaac ggttcagatt
     2161 gagcagggta actatcaggg cgcattgtca aatcttacta aaacagaagc tatagcacag
     2221 gaggttggcg atcagaaatt gctacgtaat gtatactaca attacaacag ggcttatgag
     2281 ggtttgaaga atactgaaaa agctgcagaa tattttagcc tgtatgccat gctaaccaag
     2341 aaaatacagg ccgaggaaat caggaagaaa gaattgcagg caaaagccat ggttgatagc
     2401 gcaggtaggg ttgtgcagca ggtatcacag gaaaaggagc atactgaaaa gagacttgtt
     2461 gaaacaagta aggaattaaa ggaaaaagag caaaccctca aagaggttga agagttaacc
     2521 cgtgagcagc aaatgcagat agagctttta aacgctgaga tgaagttacg cgacgcagta
     2581 attcaacatc aaaagttgct tcagaaggtt tacattggtt taatactatt ctcgttggca
     2641 tttgcagcgg ccctctacta cgcatacgtt cagaaacgca aggcaaatag attacttaaa
     2701 gagaagaatg atgaaatttc cagacagcgc gatgctatta gccgtcaggc cgatgagctc
     2761 agagagctga atgctctcaa agataagctg ttctcaataa ttgcccatga ccttaggagt
     2821 ccgcttttct cattaattac catgctcaac attgcaaagg aaggtcattt tactcctgaa
     2881 aatttcaagg aaatactcga tgagctctcg gttaacgtta accatactac agcactactt
     2941 gagaatctgt taacctgggc taaaactcaa atgcatgggg taaaggttaa tcctcagaac
     3001 ttcgacttaa atcagttggt gaattctcgt attcagctat tgaacgaggc tgctgaaaat
     3061 aaggagatca agttaaagaa cagtattccg gatggtacct ttgtttttgc cgataccgat
     3121 atgagtgata ttgtaattcg taatctgatt tccaatgcca taaaattctg caatgccggc
     3181 gataggatca caatatggag tactacctgt gcggataggg taactgtatg tgttgaggat
     3241 accggtaggg gtatgacacc cgatgtactt caaaagctat ttggtaccca aattacctcc
     3301 acgcctggaa ccaagaatga aaagggtacg ggattgggac tgattctatg taaagagttt
     3361 gtgcaaatga atggtggtga gatctgggcc gaaagccaac ccgataaggg tagcaagttc
     3421 ttctttacat tgccaaaagc cagtgtttaa aggttgttca atttctattt tggccaaatc
     3481 atttggggtt aagtttttct aacccctttt ttattgactt gaaatcttaa attttatatc
     3541 tttagaacaa atagaaaatt ctatgagtat agcccttata aaatatttat cggaatgggg
     3601 aaaacgacct agcgatatgc acaaggagcc agggccggtt gtcaccattt cgcgcgaaat
     3661 ggggtgcccg ggtaaacagg tttcttcaac actaattgag gaattaaata aaagatacag
     3721 gttgaaaaat gagttggcgt ggcgttggtt ggcaaaggag gagatacttc aaattgcttc
     3781 tgccaagctt ggtttgccca aggaggatat tgactatgtt tttgaagcaa agcgcaaggg
     3841 gataatgcag gagatactag agtcaatgtc aacaaaatac tataaaagcg ataaacacat
     3901 tcagaataca gtaaaaagca ttattcgatc cgaagcctca aaagggcatg ttgtaatact
     3961 gggaagaggc ggtgtagcaa taacccgtga tattcctcgt tccatccaca tccaccttga
     4021 agcccctttt gaatggcgag tgttacgggt tgaggaaatg taccgttttg aacccaaaga
     4081 agctgaattg tatgttcgtg aaattgaccg taagcgcgaa gagattaggg tttattttgg
     4141 cgggaaggac actgattata cccgctttga tatcaccttt aatgccatga ccctatcggt
     4201 gcatgaaatt gttgatatta taatccatgc catggaggtg cgtaacctta tcgtttaata
     4261 aagtctttag ccaacgtgtt caataacgat ctttagtccc agcccaatta gtataagccc
     4321 acctattatc tccattcttt tacccaacac gctgccaata ctttttccga aaagcatacc
     4381 aagcatggct accaaaaaag taacggagcc aattatgagg tatgcatgca gcatatttac
     4441 ctttaccgct acaaacccta tccctaccgc aaaagcatcg atactggttg ccaatgcaag
     4501 ggtaaaaagt acttttgggt ttagtggatc aagagtgctg ttgttctcct cccttccaaa
     4561 tgcctcaaca atcattttta cgccgataag gcaaagcaat gccagcgcta accagtgatc
     4621 aataggctca ataaccgatt ttatggctaa cccgaaaccc cagcccaaaa gcggcattgt
     4681 tgactgaaaa aaagcaaata ctgctgcaac cctaaaggcc tccaaaaata caatatgttt
     4741 ccgggctatt ccgcatgtga ttgatactgc aaaggtatcg aatgaaagac caattgctag
     4801 aagagtaatg gtaaa
//
LOCUS       DAOSAL010000126         4717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_11442_length_4717_cov_46.528314, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000126 DAOSAL010000000
VERSION     DAOSAL010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 4717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4717
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11442_length_4717_cov_46.528314"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1371
                     /locus_tag="PK049_11270"
     CDS             <1..1371
                     /locus_tag="PK049_11270"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HOK62180.1"
                     /translation="ISYSDVKLNAEFPKEVTDLPTNLKVEEGALNQEEEFWAQARPFE
                     LTPREANIYQMVDSVQQVPMYKTFIDIINMFVNYYYVVGYWEIGPYYQTYSFNEIEGN
                     RFKVSGRTSNKFSKRVMFDAFVAYGDKDERFKWGVGALYMISKNPREAVGVSYKSDIE
                     QLGQSPYALTEDNILTSFLRRNPNYKLTLVKDFTAYYEKEWFVGLSNKVTFSHRIIFP
                     TEFIPFTPVSGGSSLKSITNATLTLNTRWIKDERFVSGEFEKVSLGSNWPEVNFNITR
                     SIKGFAGSDYDFWKLHLNYYHKFNLNPIGYARFIVDAGKIFGKVPYPLLQLHEGNETY
                     AFDRYAFNMMNYYEFASNQYVSLYYEHHFQGFFLNHFPLLRRLKWREVATAKYLIGSI
                     SNENMQMLQFPAGLGEVSKPYMEVSVGLENIFKVVRVDALWRLTHLDNPNIEPFGVRV
                     GLQIIF"
     gene            1382..2557
                     /locus_tag="PK049_11275"
     CDS             1382..2557
                     /locus_tag="PK049_11275"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HOK62181.1"
                     /translation="MDFDFNRLISRENTSCVKYDLRQVIFGNPNVLPMWVADMDIPAP
                     DFIIDALRQRLNHPVLGYTFRDNHYHNAITWWYKHLHGWDIDTAWIDFCPGVVSGLSH
                     AIRAFTKPGDAVIIQPPVYRPFFTTVSANNRKLVLNPLIENNGQYTFNYDLLEKQVAQ
                     GARMLILSNPHNPIGKLWSKEELLKLGEICIRYNLIIVSDEIHSDLVFDGKKHFSIAS
                     LSEQLASITVTFASMSKTFNLAGLASGFVIIPNPTLKARFSAELEASGAGMGNIFGFE
                     ALKAAFTPQGEEWLSGLMQHLNSNWELLSEGLAKYGKVKVRKPDATYLVWADFRNTNL
                     TDDQLNRLLVHEAGLGFSPGTEYGAEGKGFQRINIACPNQTIELALNRLAKIARYIE"
     gene            2694..3728
                     /locus_tag="PK049_11280"
     CDS             2694..3728
                     /locus_tag="PK049_11280"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOK62182.1"
                     /translation="MRIVFISILLFISSLGDGLAGITDITGLSSKIVELNEQIRQEHY
                     GKVSSVVVLNMKGKPIFEQYYGFNSRNSLNQISSVTKSITSLALGICIDRGLIPSVDE
                     PVAKYFPEYNRQFSEYPAKKNITLKHLLNQTTGLKWDEWFFPYNYASNSLIALLESKA
                     NWIDHFFNLPVDTLAGIKFNYNSLSSQVIAEVVSRVSGMPFNQFVVENIFKPLSIDQY
                     RWDEYPNNPFPAWGGISLTTLDMAKIGLLMLNNGRYRNMQIVSVSWINNSITIQSVYN
                     QSTGYGLHWWVEHQTGEPLMYYAAGYGDQYVFVVPEKEIVIAINAQNFSDYQWPKPVI
                     DIARAIATSI"
     gene            3756..>4717
                     /locus_tag="PK049_11285"
     CDS             3756..>4717
                     /locus_tag="PK049_11285"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HOK62183.1"
                     /translation="MSKLKTNYLGIELRNPLIVGASSLTSNIDTLKAIEKAGAGAVVF
                     KSLFEEQIQLEELELENELEEYNERHAEMVKLFPTLKHAGPAEHLAKLKKAKETLGIP
                     VIGSLNAMHPDTWAEYAQAIADTGVDALELNFYTTPKDFEVEGKTIIQSQVEVLQSVK
                     EKIKIPISVKLSPFYTNPLDVIRRFDETEVDGFVLFNRLFQPDIDIDKEELVQTIILS
                     DSSDHRLPLRFTGLLYGNIAADICAATGIMNGNDAVKMILAGADAFQVVSTLYRNGID
                     HITEMLAQIEGWMNKKGYKSIDDFRGKLAKKNLHDPFAYRRAQYV"
BASE COUNT         1338 a         1000 c         1094 g         1285 t
ORIGIN      
        1 atatcgtaca gcgatgttaa gctaaacgct gagtttccca aggaagtgac agacttgcct
       61 accaacctta aagtagagga gggcgcgcta aaccaagagg aggagttttg ggcacaggca
      121 cgtccgtttg agcttacccc gcgcgaagcc aacatttacc aaatggtcga ttccgttcag
      181 caggttccca tgtataagac atttatcgat attatcaata tgtttgtaaa ctactactat
      241 gtagtaggat actgggaaat agggccatac taccaaacct atagcttcaa cgagattgag
      301 ggtaatcggt ttaaggtttc aggccgcaca agcaataagt tcagcaaaag ggttatgttc
      361 gatgcctttg tagcctacgg cgataaggat gagcgtttca agtggggggt gggagcactt
      421 tacatgatca gtaaaaatcc acgcgaagcg gttggggtta gctataagag cgatattgaa
      481 cagctggggc aaagccccta tgcgcttacg gaggataaca ttcttacatc gttccttagg
      541 cgaaacccta actataagtt gacgcttgta aaggatttta ctgcatacta tgaaaaggaa
      601 tggtttgttg gcctgtccaa taaggttacc tttagtcaca ggattatttt cccaaccgaa
      661 tttatcccat ttacacctgt ttcgggcgga agcagtctta agagcattac caatgccaca
      721 ctcaccctta atacccgttg gattaaagat gaacgctttg tttcgggcga gttcgagaaa
      781 gtgagtcttg gcagtaattg gcccgaggtt aactttaata ttaccaggtc aataaaagga
      841 tttgccggtt ccgattacga tttttggaag ctacacctaa attactacca taaattcaat
      901 ctcaacccaa ttggctacgc ccgatttatt gtggatgctg gcaaaatttt cgggaaagtt
      961 ccgtatccct tgcttcagct acacgagggt aacgagacct atgcatttga caggtatgcc
     1021 tttaacatga tgaactacta tgagtttgca tcgaatcagt atgttagcct atactacgag
     1081 caccattttc agggtttttt ccttaaccat ttcccgcttt tgcgccgcct aaaatggcgc
     1141 gaggttgcca ccgccaaata cctaattggg agcataagta acgaaaatat gcagatgctg
     1201 cagttccctg ccggcttggg tgaggtttca aagccctaca tggaggtaag tgttggtttg
     1261 gaaaatattt tcaaagtagt gagggttgac gccctttggc gcctaaccca cttggataat
     1321 ccaaacatcg agccttttgg ggttcgggtt ggattgcaaa ttatattcta aatctttaag
     1381 catggatttt gatttcaatc ggttaataag cagggaaaac acctcgtgtg tgaagtacga
     1441 tttaaggcag gtgatttttg ggaaccccaa cgttttacct atgtgggtgg ccgatatgga
     1501 tattccggcg cccgatttca ttatcgatgc actacgccaa aggctaaacc atccggtttt
     1561 gggttacacc ttccgcgata accattacca taacgccatt acctggtggt ataagcacct
     1621 ccatggttgg gacattgata cagcctggat tgatttttgt cccggggtgg tttcgggttt
     1681 aagccatgcc attagggctt tcaccaagcc cggcgatgca gtaattattc aacccccggt
     1741 ttatcggcct ttcttcacta ccgtgtcggc aaataatcgc aaacttgttt taaatccgct
     1801 gattgagaac aatggccaat ataccttcaa ctacgatttg ctggaaaagc aggtggcgca
     1861 gggcgccagg atgttaattc tttccaaccc ccataacccc ataggaaagc tttggagcaa
     1921 ggaggaactc cttaagcttg gcgaaatctg tatcaggtac aacctgatta ttgtatcgga
     1981 cgagatacac tccgatttgg tttttgatgg taaaaagcat ttctcaatcg cttcgttatc
     2041 ggagcagctt gccagcatta ctgttacgtt tgcatccatg agcaaaactt ttaaccttgc
     2101 agggttagcc agcggctttg ttattattcc taaccctacg ctcaaggcaa ggttttcagc
     2161 tgagctggag gcaagcggag caggcatggg aaatattttt gggtttgagg cattaaaagc
     2221 ggcatttaca ccgcaaggtg aggagtggct aagcggactc atgcaacacc tcaactcaaa
     2281 ctgggaactg ctcagcgagg ggcttgctaa gtatggcaag gttaaggttc gtaaacccga
     2341 tgccacatac cttgtttggg ccgatttccg taacaccaac ctaaccgatg accagctaaa
     2401 caggctttta gtacatgagg cagggttggg ttttagcccg ggaactgagt atggtgcgga
     2461 gggaaagggc tttcaacgaa tcaacatagc ttgcccaaat caaaccattg agttggcttt
     2521 aaacaggctt gctaaaatag cacgatacat agagtaatag gccaaccgtt aatcagagtt
     2581 aaaaatattt aatcgtttca ttgaaccaaa aggcgtttac atcggtttat gatgtaaatc
     2641 ctcttgcttt tatccctttt tttgaatata tttatacagt aaacggaact tatatgcgga
     2701 ttgtatttat atcgatacta ctttttattt catctttagg ggatggactt gccggtatta
     2761 ctgatattac aggactatca tctaaaatag tggagctgaa tgaacagatt aggcaagagc
     2821 attacggcaa ggtaagctca gtagttgtgc ttaatatgaa aggtaaacca atttttgagc
     2881 aatactatgg ttttaacagc aggaatagcc taaatcagat tagctcagtt accaaaagca
     2941 tcacatcgtt ggccttagga atttgtatcg ataggggttt aataccctca gttgatgagc
     3001 cggttgctaa gtattttcct gaatataaca ggcaattcag tgagtatccg gccaagaaaa
     3061 atatcactct aaaacacttg cttaaccaaa ccaccggatt aaagtgggat gaatggtttt
     3121 tcccatacaa ctatgcgtcg aacagcctga tagccctttt ggaaagtaaa gcgaattgga
     3181 ttgaccattt ttttaaccta cctgtggata ccttggcggg tataaagttt aactataaca
     3241 gtttatcgtc gcaggtaatt gctgaggtgg taagcagggt tagtggcatg ccattcaacc
     3301 agtttgttgt ggaaaatatt tttaaaccgt taagcattga ccaataccgt tgggatgaat
     3361 accccaataa tccattccct gcatggggag ggatatccct aaccaccctc gatatggcca
     3421 agattggctt attgatgctc aacaacggaa ggtacaggaa catgcagata gtttcagtaa
     3481 gctggataaa caattccata accatacaat cagtatataa tcagagcaca ggctatggcc
     3541 tacactggtg ggttgagcat caaaccggcg agcctttaat gtactatgcg gcaggctacg
     3601 gcgaccagta cgtgtttgtt gttccggaaa aagagatagt tattgcaata aacgcgcaaa
     3661 acttttccga ttaccaatgg ccaaaaccgg ttattgatat tgccagagca attgcaacct
     3721 caatttgaaa aaagtaaccc ctaataccaa tcgttatgtc aaaactcaaa actaattatc
     3781 taggaataga gctgcggaac ccgctgatag tgggtgcatc gtcgctcacg tcaaacatcg
     3841 ataccctgaa ggccattgag aaggctggag cgggtgccgt tgtttttaaa tcgctttttg
     3901 aggaacaaat ccagcttgag gagctggagc tggagaatga gcttgaggag tacaacgagc
     3961 gccatgccga aatggttaag ctatttccaa cccttaagca tgcaggcccg gccgaacatc
     4021 tggccaagct taaaaaagca aaggaaacgc ttggaattcc ggttataggg agccttaacg
     4081 ccatgcaccc cgatacatgg gctgagtatg ctcaggccat tgccgatacc ggtgtcgatg
     4141 cccttgagct gaacttttac accaccccca aggattttga ggttgaggga aaaacaatca
     4201 tccaatcgca ggttgaggtg ctgcaaagcg taaaagaaaa aattaaaatt cctattagcg
     4261 ttaagcttag cccattctat accaacccgc tggatgttat tcggcggttc gacgagaccg
     4321 aagttgatgg ctttgttctg ttcaacaggc tatttcaacc cgatatcgat atcgataagg
     4381 aggagctggt acaaaccatt atcctcagcg actcctccga ccataggtta cccttgcggt
     4441 ttaccggatt gctttacggc aatattgctg ccgatatatg tgctgccact ggcattatga
     4501 atggtaacga tgccgttaag atgattctgg ctggtgccga tgctttccag gttgttagta
     4561 cgctttacag gaatggcatt gaccatatta ccgagatgct cgcccaaatt gagggctgga
     4621 tgaacaaaaa ggggtataag tccattgacg attttagggg aaagctggcc aaaaagaacc
     4681 tacacgaccc atttgcatac cgccgtgccc agtatgt
//
LOCUS       DAOSAL010000127         4232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_13187_length_4232_cov_49.345224, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000127 DAOSAL010000000
VERSION     DAOSAL010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13187_length_4232_cov_49.345224"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(279..1106)
                     /gene="tsf"
                     /locus_tag="PK049_11290"
     CDS             complement(279..1106)
                     /gene="tsf"
                     /locus_tag="PK049_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOK62184.1"
                     /translation="MAEITAADVAKLRKLTGAGMMDCKNALVEANGDMERAIELIRER
                     GKAIANKRADREAGEGAALSKVNQAGSRGAMIVLNCETDFVAKNADFLGVANKILDHA
                     LNTAPANLDALKESSLDGRKVADFVAEFSGITGEKVELSYYEQVEAPMVVSYIHMGNK
                     LATLVGFSKAIDIQAAKDVAMQVAAMSPVAIDKDDVPEDIRQKEFEIGREQARLEGKP
                     ENMLDKIAEGKLKKFYKESTLLNQEFVKDNKLTIRQYLQSVDKDVKVTAFKRVSLNL"
     gene            complement(1137..2006)
                     /gene="rpsB"
                     /locus_tag="PK049_11295"
     CDS             complement(1137..2006)
                     /gene="rpsB"
                     /locus_tag="PK049_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOK62185.1"
                     /translation="MSRTNFQELLEAGVHFGHLKRKWNPKMAPYIFMEKNGIHIIDLH
                     KTVVKIDEAAAAVKQIAKSGRKILFVATKKQAKDIVAERIKAINMPYVTERWPGGMLT
                     NFPTIRKAVKKMNSIDKMMTDGTFDTLSKREKLQIQRQRAKLEKNLGSIANLNRLPAA
                     LFVVDVQKEYIAVREANRLNIPVIAMVDTCCDPTPIDFVIPANDDASKSIALIVDIMA
                     KAIQEGLEERKAEKDKEPAAAEKEEGVKTRARKAKKAADVVEEPEDEDEADEVEEVEK
                     PTATIVDDEIDEE"
     gene            complement(2126..2512)
                     /gene="rpsI"
                     /locus_tag="PK049_11300"
     CDS             complement(2126..2512)
                     /gene="rpsI"
                     /locus_tag="PK049_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013545814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOK62186.1"
                     /translation="MQVYNGLGRRKSAVARVYLREGKGNITINGRDLKEYFSSELLHY
                     VVKQPLMVLNATEGFDIKATLDGGGITGQAEALRLGIARALVEYNEANRAIMRANGFL
                     TRDPREVERKKPGRPKARKRFQFSKR"
     gene            complement(2521..2976)
                     /gene="rplM"
                     /locus_tag="PK049_11305"
     CDS             complement(2521..2976)
                     /gene="rplM"
                     /locus_tag="PK049_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOK62187.1"
                     /translation="MNTQSYKTVMANDATVKKEWVVIDATDQVLGRLSSQVAKILRGK
                     HKPSYTPHVDCGDNVIIINADKVRLTGRKLTDKEYVRHTGYPGGQRFSTPKEMLHRKP
                     IWVIEHAVKGMLPKNRLGAEMFRNLYVYAGSEHPHEAQQPKKIELSNIK"
BASE COUNT          994 a          925 c          995 g         1318 t
ORIGIN      
        1 tcccctttta cccttatatg agatccctcg actacgctcg ggatgacaac atcattggct
       61 ccctcattca ctttgtcatg ttgagtcccg aaatatcggg atagactcca gcgaaacatc
      121 tcatggggta gattagagat cctttgctcc ccactagggc gggacaggcc gcgcgggacg
      181 acaggcatgt atatttttgt taactaaaca acattgagtt agatataata aaaaaggaga
      241 gcaaccctgc tctccttcaa ggtgatatgc tgtaaggatt ataggttgag cgatacgcgt
      301 ttgaatgcgg ttaccttaac gtccttatca acgctttgca ggtactggcg tatggttaac
      361 ttgttatcct taacaaattc ctggttgaga agggtgctct ccttgtagaa tttctttagc
      421 ttgccttcgg caatcttatc gagcatgttt tcgggtttgc cctcaaggcg tgcctgctcg
      481 cggccaattt caaactcctt ctggcgaata tcttcgggaa catcgtcctt gtcaatagcc
      541 acagggctca tggcggcaac ctgcatggca acatccttag cggcctggat atcgattgct
      601 ttgctgaagc ctaccagagt agccagcttg ttgcccatgt ggatgtatga aaccaccatg
      661 ggtgcttcaa cctgctcgta gtacgatagt tcaacctttt cgccggtaat accggagaac
      721 tcggcaacaa aatcggctac cttgcggcca tcgaggctcg attccttaag cgcgtcgagg
      781 ttggcaggtg ctgtattcag ggcatggtca agtattttat tggctacgcc aaggaaatcg
      841 gcattttttg caacaaagtc ggtttcgcag ttgagcacaa tcatggcacc gcgggaaccg
      901 gcctggttaa cctttgaaag ggcagcgcct tcgccggctt cacggtcggc acgtttgttg
      961 gcaatggcct taccgcgttc gcggataagt tcaatggcgc gttccatatc gccgttagct
     1021 tcaaccagag cattcttgca atccatcatg cctgctccgg taagtttacg aagttttgct
     1081 acatcagcag ctgtaatttc agccatggta tttagttttt tttagggtct aagttgttat
     1141 tcttcgtcaa tctcatcgtc aacgatagtg gcagtgggtt tttcaacctc ttcaacctcg
     1201 tcggcttcat cctcatcttc gggctcttca actacatcgg cagccttttt agccttgcga
     1261 gcgcgggttt ttacgccctc ttctttttcg gcagcggcag gttccttatc cttttcagcc
     1321 ttacgttcct caagcccttc ctgaatggct ttagccatga tgtcaacaat cagggcgata
     1381 gatttggatg catcgtcgtt agcgggtatc acaaaatcga ttggagtagg atcacagcag
     1441 gtatcaacca ttgcgataac cggaatgtta agcctgttgg cttcacgaac ggcgatgtat
     1501 tccttttgaa cgtcaaccac aaaaagggct gctggtaagc ggttgaggtt ggcaattgaa
     1561 ccaaggttct tttccagctt ggcgcgttgg cgttgaattt ggagcttttc acgctttgaa
     1621 agggtgtcga aagtgccgtc ggtcatcatc ttatcgatgc tgttcatctt tttaacggct
     1681 ttgcggatgg tggggaagtt ggtgagcata ccaccaggcc aacgctcagt aacgtaaggc
     1741 atgttaatag ccttgatacg ttcggcaaca atgtcctttg cctgtttttt ggtggcaaca
     1801 aaaaggatct tacgacccga tttggcgatt tgctttactg cagcggcagc ttcatcgatt
     1861 tttactactg ttttgtgcag gtcgataatg tggataccgt ttttctccat aaagatgtaa
     1921 ggagccatct ttgggttcca cttacgcttt aagtgaccaa agtgcacacc tgcttcgagc
     1981 agttcctgaa aatttgttct tgacattttt tgtttgttta gattttttgg caatcgctca
     2041 gtagtccctt tcgggagtct gggcaattta ttactaaact gcctgccaaa tggttaataa
     2101 atagtttgtt aaacaattga tagcattaac gtttgctgaa ctggaatctc tttctggctt
     2161 ttggcctacc gggtttctta cgttcaacct cacgaggatc gcgggttaag aaaccgttgg
     2221 cgcgcataat agccctgttt gcctcgttgt actcaacaag tgcgcgggca ataccaagcc
     2281 taagagcctc agcctgaccg gttataccac caccatcgag agttgcctta atgtcaaaac
     2341 cttcggtggc gttaagtacc attagaggct gctttaccac gtagtggagc agctctgagc
     2401 tgaaatactc cttgaggtcg cggccattta tggtgatgtt gcctttacct tcgcggaggt
     2461 aaacacgggc aacagccgat tttcttctac ctaatccgtt ataaacctgc ataagttaac
     2521 ttatttaatg ttgcttaatt caattttctt tggttgttgt gcctcgtgcg gatgctcgga
     2581 accagcataa acgtaaaggt tgcggaacat ctcggcgcca agcctgttct taggcagcat
     2641 gccctttaca gcgtgttcaa taacccaaat gggcttgcga tgaagcattt ccttaggggt
     2701 tgagaaacgc tgaccaccag ggtatccggt gtggcgtaca tactccttat cggttaactt
     2761 acgaccggtt aaacgaacct tgtcggcatt gatgataatt acattatcac cacaatcaac
     2821 gtgtggggtg tagctgggtt tgtgtttccc ccttaggatt tttgctactt ggctcgatag
     2881 gcgacctagc acctggtcag tagcatcaat cacaacccac tcctttttaa cggtggcatc
     2941 gtttgccatt accgttttgt aactttgcgt attcactgca atctcctaat ttttaggtta
     3001 ataccaactt ttttgtgatc ctttaatttg ataataagcg ggacgcaaaa atacaactaa
     3061 ttatttaatt agcaagggat agcttaaaga aaaatttttg attgaactga agctgaagtg
     3121 gttagtttta gtgtttaggg tatagtgttt agtgtatagt aggtttatta ttattgtcta
     3181 gtcctaaagt aaggttccac ttaagtatga gggagcaagg tatagcctct aagcctttat
     3241 tcataactca ggaattaatg ctgtttgatt taggatattt ttcatgttta gtcccgatta
     3301 ttcggaatag attccaacga aacatctatc tgtcatgctg agctagccga agtatctctt
     3361 tgtaccttta ttcccttttt taaatgagat tcctcgacta gctcggaatg acaatacttt
     3421 tgacatccac gttccctttg tcatgctgag cttgtcgaag catctcttaa cctttaattc
     3481 ttgatgtttt ttgggcaatt gatcttggcc atacttttct gttgcgatgc aacttttcag
     3541 tgttgctctt tggaatgtaa atgcgtgcat cgctgaacat atgggagtag ccatcgggtt
     3601 acaaatattt cgcccctaca gggctgggaa ggttgtttgt tgttcgtgaa attgacctca
     3661 ccccaagccc ctctcctgac aggagagggg agaagcaata aggaaacctt attgcagggc
     3721 taaagtctcc cctctcaggg gagatttaga ggggtcgttt aggttgaata tagaaaacgg
     3781 cttcacccca gcccttttcc tgagagggaa tggagcggaa gaagcaggca gtggcataaa
     3841 agcaagacct gtaaatgggt cgggcctttc agccttgatc ttctttggtt ctttcttgta
     3901 tcaagacatg aaagaacgtg aaaaagctct ttgccatgct tatctgttgc gatgcaacgt
     3961 ttcacccttg ctctttggaa tgccatagcg tgcatcgcta tacaggtagg agtcgtgatg
     4021 gagttacaaa tgtttagccc ctacggggct ggaaacggca atcgttgttc gttattcgtg
     4081 aatcgttgtt cttgaatgcc attctgtgta acaccgtgtt acactgagta actctgtgat
     4141 aacaatttag ttgatagttg ttggttgata gttgatggtt gatggttgat agttgatggt
     4201 tgatagttga tggttgatag ttgatagttg at
//
LOCUS       DAOSAL010000128         4223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_13223_length_4223_cov_36.302303, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000128 DAOSAL010000000
VERSION     DAOSAL010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 4223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4223
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13223_length_4223_cov_36.302303"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="PK049_11310"
     CDS             complement(<1..543)
                     /locus_tag="PK049_11310"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HOK62188.1"
                     /translation="MRERALDLNEEFKGKDALQVLTWFLKEYKGRIALSSSMGAEDQV
                     LTDMVMRIDPEVKIFTLDTGRLFYETYELIERTSLRYKKNIEIYFPSPAEVERMVTEK
                     GINLFYQSIENRKECCRVRKIEPLKRAFKGLEVWICGLRRNQSATRTENQMVEWDEAN
                     GLIKLNPLIDWSEQDVWEYIK"
     gene            668..1168
                     /locus_tag="PK049_11315"
     CDS             668..1168
                     /locus_tag="PK049_11315"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOK62189.1"
                     /translation="MKKIYTVILSLFTLTAWSQDIPMQVIAAGGGYFEDSSAGLSISW
                     TLGEVAYTTLTSADFILTQGFQQGNLFTTSVDKPGITQNDITIYPNPARDYVKVKFEI
                     PNASGKATFDLYDITGRKVISKVVNVEQSVPTELNISEIRSGIYLLKVTLEKDNLNRI
                     VKLVKE"
     gene            1473..3809
                     /locus_tag="PK049_11320"
     CDS             1473..3809
                     /locus_tag="PK049_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK62190.1"
                     /translation="MKKITGMLLFMVVAITAAAQQPTGFSYQAVLRNSSGQVLANQNV
                     TLRLSITSSDGSTTYYAESHAVNTGPYGLVSLTVGEGTPLSGSLEEVPWGSNETRLKV
                     ELSTDGGSTFSSLGSQRIQPVPMAVYADSANLRFTRNLVVVGNDPTNPDDPIFEVKNS
                     KGEVLFGVYQEGVRVNIPDSAITKGAKGGFAVGGLTGAKGEPVEYLRITPDSARVYVK
                     QSPGTKGAKGGFAVGGLTNQTKAVISQDLLFVNPDSARIYINENPGKGAKGGFAVGGL
                     TNGKTSAQLIQLTKDNYLIGYEAGASITTGQFNSFLGFQAGKANSTGNWNTFLGYQTG
                     LNNTGSDNTFIGYQAGMLHQFGGGNVYLGSKAGGNALNGVQNIAIGEQSGFSTSTGFK
                     NIFIGYMAGYSNTSGYSNIFIGNESGKSNTDGNRNMFFGNGSGFSNTSGFGNTFFGLE
                     SGYYNVNGFRNLFLGYRSGYKNVDGSDNSFLGDMAGSSNTSGNENTFVGQASGASNDT
                     GRDNTFLGSNVGRGNTNGSGNVMVGRFTGYNINGNNNTFIGTQSAANFNTGPFIASSN
                     VCIGYASGTGLTNGFYNIFIGQQTGTNNGFANVIIGHQAGLNCSGNSNVFIGYQAGIS
                     EAKSNRLHISNSGGDSTQALIYGRFDSKEIALNARVGIGTTVPDKQLHVVGDARITGD
                     LYYGTGTDIYSKPDFVFKPDYSKRLDPLSVERFIARNGHLPWLTKASEERDGINLTRM
                     QFETVETVENLQLQIIQQQKEIENLKAELETLKKLIKQ"
BASE COUNT         1245 a          849 c          924 g         1205 t
ORIGIN      
        1 tttaatatac tcccaaacgt cctgttcgct ccaatcaata agcgggttaa gcttaataag
       61 gccgttagcc tcatcccact caaccatttg gttctcggtt cgggtagctg actggttacg
      121 gcgcaaacca catatccaaa cctctaaccc tttgaaagcc cttttcaagg gctcaatttt
      181 gcgaacccta cagcactcct tcctattctc aatactttgg taaaacagat taatcccctt
      241 ttcggtgacc attctctcaa cttcggctgg cgatggaaag tatatttcaa tattcttttt
      301 gtacctcagc gaggtgcgct caataagctc gtaggtttcg tagaataatc gtcccgtgtc
      361 aagagtgaaa atttttactt cagggtcaat cctcattacc atatcggtaa gcacctggtc
      421 ttctgcaccc atgctggaac tcagagcaat tctcccttta tactctttca ggaaccacgt
      481 aagcacctga agggcatctt tccctttgaa ctcttcgttt aagtctaatg ctctctctct
      541 cattgtttta attttaacac tccaaacgat aaattagaaa tacaaaactt caaattaatg
      601 taaaaaaatt gactttggta ttataattta ctaacttgct cattatgcaa atttaaaata
      661 atacaaaatg aagaaaatct acacagtaat tttatcgcta ttcacactaa cggcatggtc
      721 gcaggacata ccaatgcagg taattgccgc tggaggtgga tactttgaag actcatcagc
      781 agggctatca attagttgga cactgggcga ggttgcctat actaccctca catcagctga
      841 ttttattttg actcagggat tccagcaggg aaacctgttt accacatcgg ttgataaacc
      901 tggaattact caaaacgaca ttaccatata ccctaatccg gcaagggatt acgtaaaggt
      961 taaatttgaa atacccaatg cgagcggtaa agcaacattt gatttatatg atataactgg
     1021 acgtaaggtt atttcaaagg tggtgaatgt tgagcaatcg gtacccactg agttaaatat
     1081 atcggagata cgttcaggca tttatctgct aaaagtaacg ctggagaagg ataatctgaa
     1141 tcggattgtt aagctggtaa aggagtaact tctaatcttc ttttttgcct tttaagttca
     1201 atctactttt gttatgccaa attttagaca atgaatattt ttttcattat tggttaatta
     1261 tcaaagttag aaattaatac tctaagccag caaacaattt gttttatcag taattgaatt
     1321 aacacataaa tttttaacgt tatcattgat tttaatttgt tgagaaaatt attcgaatga
     1381 tttttcttta gaatagcttg aaataatttt gaattcagct gatatcctgt ctttctagtg
     1441 tattgtgttt aagacaaact aaaattaagg ttatgaagaa gattacaggt atgctgctat
     1501 tcatggtagt tgctatcacc gccgcagcac agcaacccac cggtttcagc taccaggccg
     1561 tgctccgcaa cagcagcggg caggtgctgg ccaaccagaa cgtgaccctc aggctgagca
     1621 taaccagctc cgatggctcc accacctact acgcggagag ccatgccgtg aacaccggcc
     1681 cctacgggct ggtaagcctc accgtgggcg agggtacgcc cctatcgggc agcttagagg
     1741 aggtgccctg ggggagtaat gaaaccaggc taaaggtgga gcttagcacc gacgggggca
     1801 gcaccttcag cagcttgggc agccagagga tacagcccgt acccatggcc gtgtacgccg
     1861 atagcgccaa cctgaggttt accaggaacc tggtggtggt gggcaacgac cccaccaacc
     1921 ccgacgatcc catatttgag gtgaagaaca gcaagggtga ggtgctcttt ggcgtatacc
     1981 aggagggggt aagggtaaat atccccgaca gcgccataac caaaggggcc aagggtgggt
     2041 ttgcagtggg cggcctgacc ggcgcaaagg gagagccggt agagtacctg cgtataaccc
     2101 ccgatagcgc cagggtatat gttaagcaaa gccccggcac caagggagcc aagggtggct
     2161 ttgccgtggg tggattaacc aatcaaacca aagcggttat atcccaggac ctgctgtttg
     2221 taaaccccga ttcggccaga atttatatca atgagaaccc agggaaggga gccaagggtg
     2281 gctttgctgt gggtggactc accaacggga agactagtgc tcaactcatc cagctaacca
     2341 aggataacta cctgattgga tacgaagctg gggcaagcat tactaccgga cagtttaact
     2401 cctttttagg atttcaggcc gggaaagcaa acagcaccgg gaattggaat accttccttg
     2461 ggtatcagac tggcttaaat aataccggaa gcgataatac attcattggt taccaggcag
     2521 gaatgcttca ccaatttgga ggtggtaatg tttacttagg gagtaaggcg ggaggaaatg
     2581 cattgaacgg tgtgcaaaat attgcaattg gcgaacaatc agggtttagt acttccacag
     2641 gatttaagaa tatttttata ggctatatgg ccggatatag caacaccagt ggctacagta
     2701 atatttttat cggtaatgag tctggtaaaa gtaataccga tggaaaccga aatatgtttt
     2761 tcggaaacgg ttcaggcttt tcaaacactt ctggttttgg caatacattt tttggtttgg
     2821 agagtggcta ctataatgta aatggtttca gaaacctatt tctaggttat cggtcaggct
     2881 acaagaatgt tgatggttct gacaactcgt ttcttggtga tatggcaggt tctagcaata
     2941 catcaggaaa tgaaaatact tttgtgggtc aggcatccgg agcctccaac gatacaggaa
     3001 gagataatac ctttttgggc tccaatgttg gtcggggtaa tactaatgga tcgggcaatg
     3061 taatggttgg gcgttttaca gggtacaata ttaatggcaa caacaacacc tttattggta
     3121 ctcaaagcgc tgcaaatttc aacactggtc cttttattgc tagtagtaat gtatgtatcg
     3181 ggtatgcaag tggaaccgga ttaaccaatg gtttttacaa tatttttatt ggacagcaaa
     3241 ctggaacaaa taacggattt gctaatgtta tcattgggca tcaggctgga ctaaactgtt
     3301 cagggaacag caacgtcttt attggatatc aggctggtat ttccgaggct aaaagtaata
     3361 gactgcatat ttccaattct ggaggcgatt caacgcaagc actaatttat ggcagattcg
     3421 atagcaaaga aattgcttta aatgcccgag ttgggatagg aacaaccgtg cccgataaac
     3481 agctacacgt agtgggtgat gcacgaataa ctggtgatct ttattatgga acgggtacag
     3541 atatttacag caaacccgac tttgtattca aacccgatta ttccaagcgg ctcgatcccc
     3601 tgagcgttga acggttcatt gctcgtaatg gccacttacc ctggctaaca aaagcatcgg
     3661 aggaaaggga tggcattaac ctaacccgca tgcagtttga aacggttgaa accgttgaaa
     3721 accttcagtt gcaaattatt cagcagcaaa aggaaattga gaacttaaag gctgaactgg
     3781 aaactttaaa aaagttaata aagcagtaat cgtttacttt tttgtgaatg ccggtcaacg
     3841 accggcatta tttttgcttt tacccaatta atggcgattg attctgtata gataatcact
     3901 actttttggc taaaaaaatt ttaagagtta gcttatacct aacttattct atatcctcca
     3961 ttgggaatag gaaatgttaa ggttggtttt tctagcaata cttcatcgcc aacggcgtaa
     4021 cgttatccta ttcccgctta gtggataaag attttttaaa tcggacagta gtgaaattgg
     4081 tagatttcta ttcgatatta ttaaaaacct tatctttaca actttgaaat atgatttttc
     4141 ttttttaaga aactaattgg agcaattagt taaaaatatt gactgattaa acgttttttt
     4201 aatatattta tcaaatctta gta
//
LOCUS       DAOSAL010000129         3937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_14516_length_3937_cov_45.832303, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000129 DAOSAL010000000
VERSION     DAOSAL010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14516_length_3937_cov_45.832303"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(346..975)
                     /gene="msrA"
                     /locus_tag="PK049_11325"
     CDS             complement(346..975)
                     /gene="msrA"
                     /locus_tag="PK049_11325"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HOK62191.1"
                     /translation="MKKLTYVLTLSALALTLMLGCKAQKPVETMSAKTETVTLGAGCF
                     WCVEAIFSRVNGVVKVESGYSGGHVVNPTYQEVCTGNTGHAEVVQVTFNPDIIPFAKI
                     LEIYFKTHDPTTLNRQGADIGTQYRSVIFYHTEDQKATAIEIKNQLDAAGIWSSPIVT
                     AIEPYKNFYKAENYHQNYYEGNKNQPYCRMVITPKLEKFEKVFKEYLKK"
     gene            complement(1297..>3937)
                     /locus_tag="PK049_11330"
     CDS             complement(1297..>3937)
                     /locus_tag="PK049_11330"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HOK62192.1"
                     /translation="HNLTACSMLMAAMITFSFNTLLAQEMSKNTCTALHVNPHPPIID
                     GALNDEVWQKDNWCSGFTQHEPHNGAEPSQQTEFMITYDNDYLYAAFRLHDTEPDKIV
                     ARVTRRDDIEGDFVAIGIDSYFDKRTAFIFCVSASGTKMDMVMTENGDREDPNWDPIW
                     WAKTARNPNGWTAEMKIPLNQLRFSAADVQTWGIQVGRHLYRNQETDFWQHVPKDAPG
                     FVHLFGELHGLKGLTPKRQVEIAPYVVTKTESFKPEEGNPFMTGRRNGLNAGVDAKIG
                     LTNNFTLDLTVNPDFGQVEADPAVINLTEFETYFEEKRPFFIEGSNLLNFPLMFGDGD
                     LATNNLFYSRRIGRAPQHYPDLGDNEYARVPSNSTILGAAKLTGRSSSGLSVGLLQSV
                     TGNAWANIDSAGNRSHEMVEPLTNYTVVSAKQEYNEGNTIVSGMVTSTNRALSGIMAD
                     EYHRNAFSGGVDLMHLWANKKYMVEAKLYGSHVNGSKDAILLTQTNSSHYFQRPDIKH
                     VKIDSSRTSLTGTGGVLQFGKVGEGHIRYMAAVAWRSPQLEINDIGYVQNVDDIFQVI
                     WVGLRYWEPTKVYRSLNVNINQWTGWNFGGESTYKGGNINLNLQLPNYWYVGGGINWN
                     GENLSVSELRGGPALKLPGGWNYWLSLNSDGRKKLIVGVSNSAYIGNYNSSVSHNYSL
                     SLTYKPLKSLLLRVSPSYSFSKSELSYVDNISTVSDTRYVRGHLNRNTWVMSLRVEYS
                     INPELSVQYYGRPYFTSGKYSRFKYITQPRAQKYTDRFLTYTDSQISYDADNEVYLVD
                     ENTDGTTDYSFDNRNFNYLNLQSNLIIRWEYRPGSTVYFVWTHGKENFESVYSNSIGN
                     DIDNLWDSHPHNIFLVKFSYRFN"
BASE COUNT         1101 a          855 c          817 g         1164 t
ORIGIN      
        1 gtcttaatcc ttgttttagt ggactttact acctgacagt actttttcca aaatcctgtt
       61 tatcaatcga ttataataat catttaacaa tattttaaac tttttagctg aaaatggggt
      121 cgtttttttg ggggtgcaaa agtacaactt ttttggcttc atgtcaacac cttttttaac
      181 ctacctgcaa gttagtgcat tttttaaaaa aataaaaaaa atgtcaaggt gtttttttaa
      241 cacatttatc aacactggtt taattgcgca aaacgctata aaacaaataa gaggagcagg
      301 tttaaactta tggattttaa cttataaatt caatataaac aactactact tttttagata
      361 ttccttaaaa accttttcga acttttccag cttgggtgtt attaccattc ggcagtaggg
      421 ttggttttta ttgccctcgt agtagttctg gtgataattt tcggccttgt aaaagttctt
      481 gtagggctca atggcagtta ctattggcga gctccagatg ccagcggcat cgagctggtt
      541 ttttatttct atggctgtag ccttttggtc ctcagtatgg taaaaaatta ccgacctgta
      601 ctgtgttcca atatcggcgc cctgtcggtt caaggtagtt ggatcgtggg ttttaaagta
      661 gatttcaagt attttggcaa agggtattat atcggggtta aaggttacct gaaccacctc
      721 ggcatggcca gtattgccgg tacaaacctc ctgataggta gggtttacaa cgtgaccacc
      781 agaataaccc gattctacct taacaacacc attcactcta ctaaaaattg cctcaacaca
      841 ccagaagcat ccagctccta aggttacggt ttcggtttta gcactcatag tttcaactgg
      901 tttttgtgcc ttacagccca acattaaggt tagggctaag gccgaaaggg ttaacacata
      961 cgttagcttt ttcatttcaa agtcaattta atcattgacc aagatattaa cgaatttgtt
     1021 agggtgttgt tcaaagtcac ctaaaaatat tgcatactaa cgtagtagga atgggacgaa
     1081 gttagtggaa gtttgagccc gtgaaccgtg aaccgtgaac cgtgaaccgt gaaccgttaa
     1141 acgtgaaacg agagacttaa ggcaggaatg agtcggaata ttgggaatgg gaggaagtta
     1201 gaggaagtta gaagaaaaga gaacagttaa accgacgtgg gataaaataa aaaaagccgg
     1261 atacaaattc cggctataca ttgcggtatc tttgctttaa ttaaatcgat acgaaaactt
     1321 aaccaagaat atgttgtgcg ggtgcgagtc ccaaaggtta tcaatatcgt tgccaatgga
     1381 attgctgtaa accgattcaa aattttcttt cccatgggtc catacaaagt aaactgtgga
     1441 accggggcgg tattcccacc gaataatcag gtttgactgt aggttcaagt aattgaaatt
     1501 cctattgtca aatgagtaat cggttgtgcc atcggtgttt tcatctacca gatatacctc
     1561 attgtcggcg tcataactta tctgtgaatc ggtatatgtc aaaaacctat cggtatactt
     1621 ttgggccctg ggctgagtaa tatacttgaa tcgggaatac ttaccgcttg taaaatatgg
     1681 cctaccatag tattgaactg acagttcagg gttaatgcta tactccaccc gtaacgacat
     1741 cacccaggtg ttcctattca gatgtcccct aacatacctt gtatcactca cagtacttat
     1801 gttatccaca taggaaagtt cacttttact gaacgagtac gatgggctaa cccttagcag
     1861 taaacttttt agaggtttgt atgtcaatga caatgagtaa ttatggctta cagagctatt
     1921 gtaattgcct atataggcac tattgcttac accaacaatt aatttcttcc gtccatcgga
     1981 gttaaggcta agccagtagt tccatccccc gggtaatttt agagcagggc cgcctctaag
     2041 ttccgataca ctaaggtttt cgccattcca gttaatacct cccccaacat accagtagtt
     2101 gggcagctga aggttcaggt ttatgtttcc gcccttatag gttgattcgc caccaaaatt
     2161 ccagcctgtc cattggttaa tattcacatt caacgacctg taaaccttag taggttccca
     2221 gtatcgcaac ccaacccaga taacctggaa tatatcatca acattttgaa cataaccaat
     2281 atcatttatt tccagctgag gcgaacgcca agcaacagca gccatgtagc gtatatgtcc
     2341 ctcgcccact ttgccgaatt gaagcacacc cccagttccc gtaagcgaag ttctggatga
     2401 gtctatcttc acatgtttaa tatcggggcg ttgaaaatag tgggatgagt tggtttgggt
     2461 tagcagaata gcatccttgc ttccattaac gtgactcccg tatagcttgg cttcaaccat
     2521 gtacttttta tttgcccata aatgcattaa atccactccc cccgagaaag cattacggtg
     2581 gtactcatcg gccattattc ccgatagtgc tctattggtt gaggtaacca ttcccgaaac
     2641 tattgtatta ccctcattgt attcctgttt tgcggaaaca acggtatagt ttgtgagcgg
     2701 ttcaaccatt tcatggctgc ggttgccagc agaatcgata tttgcccatg cattacctgt
     2761 aacactttgc agcagaccaa ccgataaacc actacttgat cggccggtta gcttggcagc
     2821 cccaagaata gttgagtttg aaggtacccg tgcgtattcg ttatcgccca aatcggggta
     2881 atgctggggt gctctaccaa ttcttcggga gtaaaataga ttgtttgtag ccaggtcgcc
     2941 atcgccaaac atcaacggaa agttaagcag gttgctcccc tcaatgaaaa agggacgttt
     3001 ctcctcaaag taggtctcaa attcagtaag atttatcaca gcgggatcgg cctcaacctg
     3061 accaaaatcg ggatttaccg ttaaatcaag ggtaaagtta ttggttaagc caattttggc
     3121 atcaactccg gcattcaaac cattgcgcct tccggtcata aaagggttcc cctcttcggg
     3181 tttaaaactc tctgttttgg ttacaacata gggtgcaatc tcaacctggc gtttgggtgt
     3241 taagcctttc aggccatgta gctcgccaaa aagatgcaca aaacccggcg catccttagg
     3301 aacatgctgc cagaaatcgg tttcctggtt cctgtaaagg tgacgcccca cctgaatacc
     3361 ccaggtttga acatcggctg ctgagaatcg gagctggttc aggggaattt tcatttcggc
     3421 agtccaaccg ttcgggttcc gtgcagtctt agcccaccaa attggatccc agttagggtc
     3481 ctccctatcg ccattctcgg tcattaccat gtccatcttt gtacctgaag ctgaaacgca
     3541 gaaaataaat gcggttcgct tgtcaaagta ggagtcaata ccaattgcaa caaaatcgcc
     3601 ctcaatatca tcgcgacggg taacgcgtgc tacaatttta tcgggctctg tatcgtgcaa
     3661 ccggaaagca gcataaaggt aatcgttatc gtacgtaatc ataaactccg tttgctgcga
     3721 tggttcagca ccattatggg gttcatgctg ggtaaatcca ctacaccaat tatctttttg
     3781 ccaaacctca tcgttcaatg ccccatcaat tataggggga tggggattaa catgtaacgc
     3841 tgtgcatgta ttttttgaca tttcctgggc caacaatgta ttgaatgaaa aagtaatcat
     3901 tgctgccatc aacattgagc atgcggtaag gttatgc
//
LOCUS       DAOSAL010000130         3708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_15796_length_3708_cov_61.354229, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000130 DAOSAL010000000
VERSION     DAOSAL010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15796_length_3708_cov_61.354229"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            293..2857
                     /locus_tag="PK049_11335"
     CDS             293..2857
                     /locus_tag="PK049_11335"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005821819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HOK62193.1"
                     /translation="MSKDISLMEQQAKQKVKTYTFEEAYEASIKYFKGDEMAAKVWVS
                     KYALKDSFGTIYELTPDDMHWRLAREIARIERKYPNPMSEEEVYHLLKDFKYIVPQGG
                     PMTGIGNKFQIASLSNCFVIGNNNADSYGGIMKVDEEQVQLMKRRGGVGHDLSHIRPK
                     GSPVLNSALTSTGVVPFMERYSNSTREVAQDGRRGALMLSISIKHPDAEKFIDAKMEQ
                     GKITGANVSVKIDDEFMRAAIEGKPYVQQYPIDSSNPKFRQEINASELWKKIVHNAWK
                     SAEPGILFWDTIIRESVPDCYADLGYKTVSTNPCGEIPLCPYDSCRLLALNLYSYVDN
                     PFSPQATFNFELFKKHVHAAQRMMDDIIDLELEKIDAILEKIANDPEDDEIKSVERNL
                     WLKIRRKTIEGRRTGIGITAEGDMLAALNLRYGSDDAIDFAVEVHKSLAVEAYRSSVY
                     TAKERGAFPIFNAQREKDNPFINRIKAEDEELYKEMTTYGRRNIALLTIAPTGTTSLM
                     TQTTSGIEPVFMPYYKRRRKVNPNDKNVKVSFVDKVGDSWEEFNVFHHKFLVWMEVNG
                     YNPEEVKNYDDEQLSELVQKSPYYKATSNDVDWLSKVRMQGMIQKWVDHSISVTVNLP
                     EDITEEMVAKVYQTAWESGCKGCTVYREGSRDGVLVTNKKEKEQATSSAFPSKRPPVL
                     ECDVIRFKNSHEDWVAFVGLYDGRPYEIFTGRTEDDVLPIPKAVTKGRIIKIKDENGS
                     RYDFQYVDKYGYTNTIGGLSHMFNKEFWNYAKLISGVLRNGMPIPDVVNLVSSLRLDN
                     ESINSWIAGVERSLRRYIPDGTKAKPGKKCPECGSESLIYQEGCLTCKNCGHSKCG"
BASE COUNT         1074 a          770 c          872 g          992 t
ORIGIN      
        1 tccaaattga gggcgaaggc aacttcgccc tcttttcttt tccgcatttt atcaggctct
       61 gcggtttaat gtgttgagtc tcagaaaccc tagtaatttc aagtgattca atattttatc
      121 ctgttaataa ctatgaacgg ttgttaatat ttacaaccgt ttttttttgg ttcgggtaag
      181 cccaattcga taaattcgta attaaaattt caaaaacaaa aaattgctta atcaactcac
      241 agccaaagct gtgatatgga atttttgtca ataaagtaaa aaatatataa ccatgagtaa
      301 agacatttcc ctgatggaac agcaagcaaa gcaaaaggta aagacttaca cctttgagga
      361 ggcctatgag gcaagcatta agtatttcaa aggcgacgag atggctgcaa aggtttgggt
      421 gagtaagtac gcccttaagg actcgttcgg aacaatttat gagttaaccc ccgatgatat
      481 gcattggcgg ctggctcgcg aaattgcacg cattgagcgg aagtacccaa accccatgag
      541 tgaggaggag gtttaccact tgcttaagga ttttaagtac atagttcccc agggtgggcc
      601 aatgacaggt attggaaaca agtttcagat tgcatcgcta tcaaactgtt ttgtaattgg
      661 taataacaat gccgactcgt acggtggaat aatgaaggtt gacgaggagc aagttcaact
      721 catgaagcgc cgtggcggcg tggggcacga cctttctcat attcgcccca agggcagtcc
      781 ggtgcttaat agtgcactta catcaactgg agtagttccc tttatggaac gctactccaa
      841 ctcaacccgc gaggtggctc aggacggtcg acgtggcgca cttatgctgt caatctccat
      901 caagcatccc gatgccgaaa agtttattga tgccaaaatg gagcagggaa agataactgg
      961 tgccaatgtt tcagtaaaga ttgacgatga gttcatgcgc gcggccattg aggggaaacc
     1021 ttacgtgcag caatatccaa ttgactcatc aaatccaaaa ttccgtcagg aaattaatgc
     1081 tagcgagctt tggaaaaaga ttgtacataa cgcttggaaa tcggctgagc ccggtatact
     1141 tttctgggac accattatca gggaatcagt tcccgattgc tatgccgatt tgggctacaa
     1201 aaccgtatcg accaatccat gcggtgagat tcccctttgc ccatacgata gctgccgcct
     1261 actggcgctc aacctttata gctatgttga taatccattc tccccccagg caactttcaa
     1321 ctttgagctt ttcaagaagc atgtgcatgc agcccagcgc atgatggacg atataattga
     1381 ccttgagctc gaaaaaattg atgctatact tgaaaagata gccaatgacc ctgaggatga
     1441 tgaaattaaa tcggttgagc ggaacctatg gctaaagatc cgcaggaaaa ccattgaggg
     1501 acgaagaact ggcattggta taaccgctga gggcgatatg ttggccgctt taaacttacg
     1561 ctatggctct gacgatgcaa tcgattttgc ggttgaggtt cacaaatcgt tagcggtgga
     1621 agcatatcgt tcatcggttt acaccgcaaa ggaacgtggt gcattcccaa ttttcaatgc
     1681 acagcgcgag aaggataatc cattcattaa tcgcattaaa gcggaagatg aggaattata
     1741 caaggagatg actacttatg gacgtagaaa cattgccctg ctaaccattg ctcctaccgg
     1801 aaccacaagc cttatgactc aaaccacatc gggtattgag ccagttttta tgccttacta
     1861 taagcgtcgc cgtaaggtta accccaacga taagaatgtt aaggtgtcgt ttgttgataa
     1921 ggtaggcgat tcatgggagg agtttaatgt attccaccat aaattcctgg tttggatgga
     1981 agtgaatgga tacaaccccg aagaggtaaa gaattacgac gacgagcaac taagcgaact
     2041 ggttcagaaa tcgccctact ataaggcaac atccaacgat gttgactggc ttagcaaggt
     2101 tcgcatgcaa gggatgatcc aaaagtgggt tgaccactcc attagcgtta cagttaacct
     2161 ccccgaagat attaccgagg agatggttgc aaaggtttac caaacagcct gggagagcgg
     2221 atgcaaaggg tgcactgttt accgcgaggg atcacgcgat ggcgtacttg ttaccaacaa
     2281 aaaggagaag gaacaagcta catcatcggc attcccaagc aagcgtccgc cagtactgga
     2341 gtgcgatgtt atcaggttta aaaatagcca tgaggattgg gttgcattcg tgggacttta
     2401 cgatggccgt ccgtatgaga tttttactgg tagaaccgag gatgatgtgc tgcctatccc
     2461 caaagcagta acaaagggta ggattatcaa gataaaggat gaaaacggta gccgctacga
     2521 tttccagtat gttgataagt atggctatac taacaccatt ggtggtcttt cgcacatgtt
     2581 caacaaggag ttttggaact atgccaagct gatatcgggt gtactccgaa acggaatgcc
     2641 catacccgat gtggttaacc tggtttcatc gttaaggctc gataacgaaa gtattaactc
     2701 atggattgct ggtgttgaac gttccttgcg ccgttatata cccgatggaa caaaggcaaa
     2761 acccggcaag aagtgtcctg agtgtggttc tgagtcgctg atttaccagg agggttgttt
     2821 gacttgtaag aactgtggac actcaaaatg cggatagtat tttatagaac tataaaaaac
     2881 cgcctgtata ggcggttttt ttatggggta atgtttggat taaagcagtt taccgtatat
     2941 actctttctg gaattatgtg gggtgagctg attcaatcat gtactataca ataatggatg
     3001 ttggatattt agggattagt gtcgcttagt tgaaaaaact ctctttgtca tcctgagtgt
     3061 agcgaaggaa ttcattatta gtttactggg ttcagtgtgc agtgtacagt agggttggtg
     3121 tttagtgttt agtgaaaatc tgcgtaaacc accctaatct gtactatctg tgttttattg
     3181 aattttctat caactgtaaa ccaacaacta aataggtatc acagagttac acgatgtaac
     3241 acggtgttac acagagttta cacgatgttt ctcagtgtaa atctgcctag tccgtgtcat
     3301 ctgtgttcca tctcagtttt cacgaacaat gattcacgaa caacgtacaa cgtccgctag
     3361 ccatgtaatg aaaaatcaac ttagcccagg gttttaaccc tgggtattgt ggtattcaaa
     3421 atatttggcg atgcacgcta tgcatttgcc aaagagcaac accgaatcgt tgcatcgcaa
     3481 agggaagtca tggcaaagag ctatttacac gttctttctt gtcttgatac aagaaagaac
     3541 caaagaaaat caaggctgaa aagcccgacc cgatgggtac cccgtgggtg gaactgccac
     3601 gcgatactat tcgccactcc ttcggagtga ctcatatggt atcgcttact agacccaccg
     3661 ccacgttcca cccccgaccc attgccgggt caggctttta tgccactg
//
LOCUS       DAOSAL010000131         3498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_17145_length_3498_cov_46.508278, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000131 DAOSAL010000000
VERSION     DAOSAL010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17145_length_3498_cov_46.508278"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            216..1319
                     /locus_tag="PK049_11340"
     CDS             216..1319
                     /locus_tag="PK049_11340"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOK62194.1"
                     /translation="MQRLAHLLLAVLVLVLLSSWGGTGHYIISLEASRSFNTEMQQFA
                     DWANYLAGHASDADNRKSSDPTEAPKHYIDIDNYTEFFANQPMPLSLSEAVSKYGHSF
                     VVDNGILPWATKATYDSLVIALRNGRIDRAKLLAADLGHYVADGHMPLHLTKNYNGQL
                     TGNTGIHSRYESTMINAHADEFYNSQPREIVLVQNVQQYIFDYIYFNYQYVDSILEAD
                     NYARSINPDYSSSAYRDTLWVKTKGFTKRLFAAASQRLAELIYSAWVEAGRPSVTSAA
                     EVNLDRATMYVYPNPTSGQVNVTYSNSGGENYYLEIFDMLGNTLKRYSLNSISSSSGH
                     LTENLNFPSGAYLFRLSNNNISITQRVIVQKND"
     gene            1378..2118
                     /locus_tag="PK049_11345"
     CDS             1378..2118
                     /locus_tag="PK049_11345"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOK62195.1"
                     /translation="MKKSDLAIISIGVVIAFGLCGYLIGQSIENFRNGDRTILVKGFA
                     EREVKADLAVWSISCRAASNDLVEGAKRIEQDQKKIIDFLVDKGIGKEEIISREIRVN
                     DRLANEYGGYDNVNFRYVIVKVLQVRSTNVDRVQEVSRLTDELVRAGVVISQNDYSNN
                     LQYYFTKLNDIKPEMLTEATQNAREAAISFASESGVKLGKLKRASQGLFTIVDRDQSL
                     SGGDSFASGNSDIVKRVRVVISVEYSIK"
     gene            2150..3061
                     /locus_tag="PK049_11350"
     CDS             2150..3061
                     /locus_tag="PK049_11350"
                     /inference="COORDINATES: protein motif:HMM:NF013761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HOK62196.1"
                     /translation="MFNKLIASMLPYFPKKLVWIFSKRYIAGETIADAIRVSKELNAQ
                     GIKVTIDILGEFIKDLGEAERNKKEYLELIDTIQKENIDGNYSLKPTSFGLLIDKEIC
                     YNHIREIVAKAASYGNFIRIDMEDSPCTSMEIELYRRLKAEFPKNVGLVFQAYLKRTL
                     KDLQDLMDIHTDEHPNNYRLCKGIYVEPAEIAYKKYEEINQHFLEDLEFLLKNGNYVG
                     IATHDKPIIEGAYKLIEKYNVPKNKYEFQMLYGVTPELRKSIVDKGHTMRVYVPFGKE
                     WFGYSTRRLKENPKMASHIIKALFFRG"
BASE COUNT         1012 a          716 c          817 g          953 t
ORIGIN      
        1 acgctacgcc tcagccagag cataaaaagc tttttgtacg agcctgaacc aagggaattt
       61 tttactacat ttactgaaag caatggggca gagttgtgaa taccgcacgc gtttaaaaac
      121 gagcgggaac gagagtttac agacgcgcgc aagccagagt attcaccaag ttaaaccata
      181 ttgtccgtat tcaatcaaaa ccttaaaata gcattatgca aaggttagca catttattac
      241 ttgcagtttt ggttttggtt ttattgtcgt cgtggggtgg aaccgggcat tacatcatta
      301 gccttgaggc aagccgatcg ttcaacacgg aaatgcaaca gtttgccgat tgggcaaact
      361 acctggccgg ccatgcatcg gatgccgata accgtaaaag ttccgatcct actgaggctc
      421 caaagcatta catcgatatt gataactata cggaattttt tgccaaccag ccgatgcccc
      481 tgagtttgtc cgaagctgta agcaagtatg gccatagctt tgtggttgac aatggtatat
      541 tgccctgggc aaccaaagca acctacgatt cactggttat tgctttgcgg aacggcagga
      601 ttgatagggc taagctgctg gccgccgatc ttggtcacta tgtggccgat gggcatatgc
      661 cgctgcatct gaccaaaaac tacaatggtc agcttaccgg caacaccgga atacactcca
      721 ggtatgagtc aacaatgatt aacgcccatg cagatgagtt ctataatagt cagccccgtg
      781 agatagtctt agttcagaat gttcagcaat acatatttga ttacatctac tttaactatc
      841 agtacgtaga ttcaatactg gaggccgaca actatgccag gagcatcaat cccgattaca
      901 gctcatcggc atacagggat actctttggg taaagacaaa aggttttaca aaaaggttat
      961 ttgcggctgc atcgcaacga ttggctgagc tgatttacag cgcgtgggtt gaggccggaa
     1021 ggccatcggt aacaagcgct gccgaggtta accttgatag ggctacaatg tatgtttatc
     1081 cgaatcccac cagcggacaa gtaaatgtca cctatagcaa ttcgggcggt gaaaattatt
     1141 acctggagat tttcgatatg cttggcaata ctttaaaacg atacagtttg aattcaatat
     1201 cgagctcatc gggtcaccta accgaaaacc tgaattttcc ctcaggggct tacctcttta
     1261 ggttgagcaa caataacatt agcattactc agagagttat agtccaaaaa aatgattgaa
     1321 atcatggttt aatcattggt tattaaacta actttgcatt aagcaaacgc attgaccatg
     1381 aaaaaatcgg atttagcgat aatatcaatc ggagtagtta tagcgtttgg gctatgcggg
     1441 tatttaatag gccagtcgat tgaaaacttt aggaatggcg accgtaccat tctggtaaaa
     1501 ggttttgccg agcgtgaggt aaaagccgat ttggcagtgt ggagcataag ctgccgtgcg
     1561 gccagcaacg atttggttga aggggccaag cgtattgagc aggatcagaa gaagatcatc
     1621 gatttccttg tggataaggg aattggcaag gaagagatta tcagccgcga aattcgtgtt
     1681 aacgacaggt tagccaacga gtatggcggc tacgacaatg taaactttag gtatgtgatt
     1741 gttaaggtgc tccaggttcg aagcacgaat gtggacaggg tacaggaggt aagccgccta
     1801 accgatgagc ttgtccgtgc cggtgtggtt ataagccaga acgattactc caataacctc
     1861 cagtactact ttaccaagct aaacgatatt aagcccgaaa tgcttactga ggctacccag
     1921 aatgccaggg aggcagcaat ctcctttgcc agtgagagtg gagttaagct tggcaagctt
     1981 aaaagggcaa gccaggggct gtttaccatt gtcgacaggg atcagtcgct ctcgggtggc
     2041 gatagctttg cctcaggtaa cagcgatatt gtaaaaaggg ttagggtagt aattagcgtt
     2101 gaatactcaa taaagtaacc ataagtttaa ccattaattt tgaattgtta tgttcaataa
     2161 gttgattgca tcaatgcttc cgtactttcc caagaagctg gtgtggattt tttcaaaacg
     2221 ttacattgcc ggcgaaacca ttgccgatgc cattagggtt tccaaggaac tgaatgctca
     2281 agggataaag gtaaccattg atattctagg tgaattcata aaggatttag gtgaggccga
     2341 acgtaacaaa aaggagtatc tggagcttat tgataccatt cagaaggaaa acatagatgg
     2401 taactactca ttgaaaccca cctcgtttgg gctgttaatc gataaggaga tttgctacaa
     2461 ccatatccgt gaaattgttg ctaaggctgc gtcgtacggt aatttcatcc gtatcgatat
     2521 ggaggactcg ccctgcacca gcatggagat tgaactctac cgtaggttaa aagccgagtt
     2581 ccccaaaaat gttggtttgg tattccaggc atacctaaag cgtacgctta aggatttgca
     2641 ggatttaatg gatatccata ccgatgagca tcctaacaac tataggcttt gcaaggggat
     2701 ttatgttgag ccggctgaaa ttgcctacaa aaaatacgaa gagattaacc agcatttcct
     2761 tgaggatcta gaattcctat tgaaaaacgg caactatgta ggtattgcta cccacgataa
     2821 accaattatt gagggagcgt ataagctgat tgagaagtat aatgtaccca agaataagta
     2881 tgagttccag atgctttacg gtgttacccc tgagctccgg aaatcgatag tagataaggg
     2941 gcacaccatg agggtttacg tgccatttgg caaggagtgg ttcggttact caacccgtcg
     3001 actcaaggaa aatcctaaaa tggctagcca tatcatcaag gcactctttt tcaggggata
     3061 ataacgcata ttgttttcaa aagttccggt gtattccgga gcttttattt ttccttatcg
     3121 tacaaactgc gcattgagtg taggatgttt gttagctacc agtagtgcaa taccaaatgc
     3181 catcctgagc cccgatatat tgggatagac ttcgccgaag tatctcattg ctcatttcta
     3241 ttcaatcctt tgaagagatc cctcgacttt gctcgggatg acaaaaacca tatcttcctt
     3301 tctgctccct tgtcatgctg agctagccga agcatctctt tacctttaat tcttgctgtt
     3361 tttttggaaa ccgctcttag ccatgctatt ctgttgcgat gcaacgtttc agtgatgctc
     3421 tttggaatgt aaatgcgtgc atcgctatac atttaagaat aatcatggtg ttacaaatat
     3481 tacgccccta cggggctg
//
LOCUS       DAOSAL010000132         3495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_17161_length_3495_cov_55.846512, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000132 DAOSAL010000000
VERSION     DAOSAL010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17161_length_3495_cov_55.846512"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="PK049_11355"
     CDS             <1..629
                     /locus_tag="PK049_11355"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOK62197.1"
                     /translation="IAFLFTLLIWLVKLVEIIEGSSLYRLGLRPRSISGLIGIFTAPL
                     IHSDFNHLINNTITIFILIAGLVYFYRDLWYKILSLSWLLSGMMVWIAARASYHIGCS
                     GIIYAIASFIFFSGVFRKNINLMAISLLVVFLYGGLVWGVLPIFPQISWEYHLFGGIT
                     GAILAFVYRRQGPPAQTWSWMSETADDAEETSEYWESEIFDESDKEKL"
     gene            675..1055
                     /locus_tag="PK049_11360"
     CDS             675..1055
                     /locus_tag="PK049_11360"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOK62198.1"
                     /translation="MAKINEPEFNANNINLVGFGTEINGDIKSSGDLRIDGTLVGNLN
                     VKGKVVVGETGKIKGELVCKNSDISGVVEGKITVDELLSLKSSARINGDITVGKLAIE
                     PGCKFTGYCNMLGMESKEVIPGQE"
     gene            1416..2501
                     /gene="atpB"
                     /locus_tag="PK049_11365"
     CDS             1416..2501
                     /gene="atpB"
                     /locus_tag="PK049_11365"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOK62199.1"
                     /translation="MKKRINLSVLILLLLIAASINCAQADEVHKKDSKGFNTAEFIFD
                     HVSDSYEWHILTVNHHHISIPLPVILYSKKTGLHVFLSNKFHHDHGVYKGFRIETEGK
                     LKGKIVELNNRGEVDETNPLPYNFSITKNAFSILFTSILLALIFIGIARRYKKDPDSP
                     PKGLQNVFEPFIIFIRDEIAIPSIGEKRYNHFMPFLLTLFFFIWFSNLLGLIPLFPGG
                     ANVTGNISVTMALALFTFFTINRHANKDYWKHIVNPPGIPTFLKLPIPIMPLVEFIGV
                     FIKPFVLMVRLFANILAGHMVATVLLSLIFIFSSMIAWLGFAVAPISLVFLIFMSLLE
                     LLVALIQAYVFTLLTALYIGMATEEHH"
     gene            2520..2777
                     /gene="atpE"
                     /locus_tag="PK049_11370"
     CDS             2520..2777
                     /gene="atpE"
                     /locus_tag="PK049_11370"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOK62200.1"
                     /translation="MNTLLILLQSIDGASIAKFGIALGAAIIVLAAGLGISRIGSSAL
                     KSIARQPEASGDIRTNMIVSAALIEGVAFFALIILIVALFV"
     gene            2789..3283
                     /gene="atpF"
                     /locus_tag="PK049_11375"
     CDS             2789..3283
                     /gene="atpF"
                     /locus_tag="PK049_11375"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HOK62201.1"
                     /translation="MGLVTPDYGLLFWALLSFGVLLFILKKFAWKPILQALKNREESI
                     ARSMRLAEQARAEIEKLQEESKNMAEKARQEREREIAETKALREKLLAEAKEQARIET
                     QKMLDRAREQILLEKEAAKKELYVTVAELTISLTEKVLRTQLKDKEKQQEYIERLLDE
                     IPKN"
     gene            3287..>3495
                     /locus_tag="PK049_11380"
     CDS             3287..>3495
                     /locus_tag="PK049_11380"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HOK62202.1"
                     /translation="MISGSIASRYAKGLFEFCQENNELESIYPTIRHLLEILSKESKL
                     HEVLEDITLTPDEKISLIERVSGNT"
BASE COUNT         1040 a          629 c          690 g         1136 t
ORIGIN      
        1 aaattgcttt tctgtttacc cttttgattt ggctagtcaa gctggtggag ataattgaag
       61 gctcatcgtt atatcggcta ggccttcgac cccgtagtat ttcaggttta ataggaatat
      121 ttaccgctcc tttaattcac tctgacttta atcatctaat aaataacaca ataacaatat
      181 ttattttaat tgcaggttta gtttattttt acagagattt atggtataaa attctttccc
      241 taagctggtt gttaagcgga atgatggttt ggattgctgc tcgcgctagt taccatattg
      301 gttgcagcgg tattatatat gctattgcat cgtttatctt ttttagtgga gtttttcgca
      361 agaacataaa cctgatggca atatcccttt tggttgtttt tctttatggt ggattggttt
      421 ggggcgtact accaatattc cctcaaattt cgtgggaata ccatttattt ggaggaataa
      481 ctggtgcaat actcgctttt gtttaccgaa ggcaaggacc ccctgcacaa acctggagct
      541 ggatgagcga aactgctgat gatgctgaag aaacttcaga atattgggaa agtgaaattt
      601 ttgacgaaag cgataaggaa aaactttaaa cggaattttt atatttgtaa ttctaaacat
      661 taaacttttt acctatggca aaaataaatg aacccgaatt taatgctaac aacatcaacc
      721 tggttggatt tggcactgaa atcaatggcg atattaaaag tagtggcgat ttgcgtattg
      781 atggcactct ggttggtaac ctcaatgtta aaggcaaagt tgttgttggg gaaaccggta
      841 aaataaaagg tgagttggtt tgcaaaaact cagatatttc aggtgttgtt gagggtaaaa
      901 tcactgtcga tgagctatta tcgctcaaat catcggcacg gataaatggc gatattactg
      961 taggtaaact tgctattgaa cccggttgca agtttacagg ttactgcaat atgctaggaa
     1021 tggaaagcaa agaagttatt ccaggtcaag agtaaatcaa atgagtgtta aacaaagatt
     1081 tgcgattact cttttagtta tagttttatc cttactattg gttctatggt tcgttaccag
     1141 tgaaacatta gtaaggataa tacctttagt tcctgcttct ttgagtttta tagccttcgc
     1201 attaatctca tcaaaatcag ttttaccaag aattaagtat gtaagtaagt attttatcat
     1261 taagtcaatc aatgttttag taaacctcgt agctgtctta gtaatttttt tagccggttt
     1321 tagtcacaaa attctgttgg ttatcgtata tttgatatcg tatctggttt tacttttgca
     1381 tttcacatac tttattgtaa aggataggaa tacaagtgaa gaaaagaatt aacctatcgg
     1441 ttttaatttt attgttactc attgccgcaa gcataaattg cgcgcaagct gacgaagtac
     1501 acaaaaaaga ctctaagggg tttaatactg ccgaattcat atttgaccac gtaagcgatt
     1561 cgtatgagtg gcatatactt accgtaaatc accatcatat tagcattccg cttccagtaa
     1621 tactttacag caaaaaaaca ggcttgcatg tatttctttc caacaagttc catcatgatc
     1681 atggcgttta taaaggtttc aggattgaga cagaaggaaa acttaaaggt aaaattgtgg
     1741 agttaaacaa taggggagaa gttgacgaaa ccaatccatt accctataac ttttccatta
     1801 ccaaaaacgc cttttcaata ctattcacat caattttgtt ggctttaatt ttcattggca
     1861 tagcccgaag gtataagaaa gatcccgatt caccacccaa aggtttacaa aatgtttttg
     1921 agccatttat aatatttatt cgggacgaaa tagcaatccc ttctatagga gagaaacgct
     1981 ataaccattt catgcccttt ttgttaactc ttttcttttt tatatggttc tcaaatcttt
     2041 tgggtcttat tccattgttt ccaggggggg ctaatgttac tggtaacata tccgtaacca
     2101 tggctcttgc gctttttact ttttttacaa ttaaccggca tgccaacaaa gattactgga
     2161 aacatatagt taacccaccg ggtataccaa catttttgaa gttgcccata ccaatcatgc
     2221 ccctggtaga gtttattgga gtattcatca agccatttgt tttaatggtc cgtttgtttg
     2281 ccaatattct tgctgggcac atggtagcta cggttctgct tagcttgatt ttcatttttt
     2341 cttcaatgat agcttggtta ggatttgccg ttgcacccat ttctctggtt ttccttatat
     2401 tcatgtcgtt gctggaatta ctggttgccc ttatccaggc atatgttttt actttgctta
     2461 cagccctata tattggaatg gctacagaag aacatcacta acaacttaaa tataaaacta
     2521 tgaatacatt actaatttta cttcagtcaa tcgatggcgc atcaattgca aaatttggca
     2581 ttgcattggg tgcagcaata attgttttag ccgcaggact gggcattagt cgcataggct
     2641 cttcggctct aaagtctata gcccgtcagc ctgaagcctc aggcgatatt cgaacaaaca
     2701 tgattgtttc ggctgcgctt attgaagggg ttgcattttt tgcccttatt atacttattg
     2761 tggctttatt tgtataaaat tttagcctat gggtcttgtt acacccgact acggcctgtt
     2821 attttgggct ctgctatcgt ttggggtgct gctttttatt cttaaaaagt ttgcatggaa
     2881 acccatactg caagccctta aaaacaggga ggaaagcata gcgcgttcaa tgcgccttgc
     2941 agagcaggcc cgtgcagaaa ttgaaaagct gcaggaagaa agtaagaaca tggccgaaaa
     3001 ggctaggcaa gaacgcgaac gcgaaatagc cgaaactaaa gctttgcggg aaaaactgct
     3061 ggctgaagca aaagagcagg ctcgtattga aacccaaaag atgctggata gggcccgtga
     3121 gcaaattctt ttggaaaaag aggctgccaa aaaggaactt tatgttactg ttgcagagtt
     3181 aaccataagc ctaactgaaa aagttttacg tacacagctt aaggataaag aaaagcaaca
     3241 ggagtacatt gagcgtttgc tcgacgaaat acccaaaaac taaaaaatga tttccggatc
     3301 aatagcatcg cgttacgcta aagggctttt tgagttctgt caggaaaaca atgaacttga
     3361 aagcatttat cccactattc gtcatttact tgaaatcctc tctaaagaat cgaaactgca
     3421 cgaagtgctt gaagacatta ctcttacccc tgacgaaaaa attagtctga ttgaacgcgt
     3481 ttccggaaat acttt
//
LOCUS       DAOSAL010000133         3318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_18396_length_3318_cov_52.587190, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000133 DAOSAL010000000
VERSION     DAOSAL010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18396_length_3318_cov_52.587190"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="PK049_11385"
     CDS             <1..555
                     /locus_tag="PK049_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK62203.1"
                     /translation="IPSAYQQYVKNGGTYSFKRWLYSGEVFSFEHLIYSRLIEHYINL
                     FGEKQVKVFLYEDFKRNNREFMENFCQQFGMEVDWDKIDFNPTNRGLRLLSLPLLKVV
                     NHFYKKPVGRKHFICHIPGMTTVGRGVIKYLNPMPVFGRYLNEDDFLSKKDLEYIKNF
                     YSQHNCELTKYFDRRVLEGYGYYL"
     gene            552..1460
                     /locus_tag="PK049_11390"
     CDS             552..1460
                     /locus_tag="PK049_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK62204.1"
                     /translation="MKSPILHIGYPKTATKWFQNEFYPNVSGVYPVMRKDLFDRLVFT
                     DIYSYSPSHFRGWITGEAGSKRLIICDEILVGGLDIGFGNGEFVLLMSQRLHDTFPDS
                     QVVILIRNQHTALESAYSHYVMAGGTYSPSRFLGIKPMFIKPYMGYNQFSTKLFEYDK
                     LIDLYISLFGKDKVHIFLYEDFAENPNRFIALFCNTLQLDAPQGQTLNRLNARYSYIA
                     LQIQRFLNRFALGNTPFKQYFINFPSIYKISRSLILATDKYCSFPKYKFDKKVHHWVE
                     LRFKESNQCLSQWVSKEKLLRWGYPL"
     gene            1558..2802
                     /locus_tag="PK049_11395"
     CDS             1558..2802
                     /locus_tag="PK049_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOK62205.1"
                     /translation="MEVQSIKQRFGIIGNSPSLNRAIDIARQVAPTDLSVLITGESGT
                     GKEVFPQIIHNHSARKHGAYIAVNCGAIPEGTIDSELFGHEKGAFTGATDSRKGYFEV
                     ADGGTIFLDEVGELPLSTQVRLLRVLETGEFIRVGSSKVQKTNVRVVAATNVNLVDAI
                     AENKFREDLYYRLNTVPIFIPPLRERQDDIPLLFRKFATDFAEKYRMPAIALDDEARM
                     VLISYYWPGNVRQLKNVTEQISIIEQKRVIDSETLLRYLPDYNASRLPTLYHKKGVVD
                     EQTFATEREILYKILYDMRSDMNELKKLVFDLMRNGVVSPEHVRSSPVFQDLGVTHVD
                     PIVSSAPARGTETKSFVKDLNDIQDTEEIQEETLSLEEKEKELIVKALEKHNGRRKWA
                     AMELGISERTLYRKIKEYNINL"
     gene            2805..>3318
                     /gene="lptE"
                     /locus_tag="PK049_11400"
     CDS             2805..>3318
                     /gene="lptE"
                     /locus_tag="PK049_11400"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HOK62206.1"
                     /translation="MSRKKFIIPLILVFTLISQNSCKVSYSFTGASIPLEAKTYSVQL
                     FQNLASIVNPTLANYITEELKNRFLTQTRLTPVADFGDFAFSGQIVTYDVSPMAIQGD
                     EIAAQNRLTIAVKVKFVNAIDPKQSFDKTFSQYAVFSSSQNFSLVEQALVQEIVEKLI
                     DDIFNAAASNW"
BASE COUNT          949 a          643 c          748 g          978 t
ORIGIN      
        1 ataccatcgg cataccagca atatgtcaaa aatgggggta catatagttt taaaagatgg
       61 ctatactccg gtgaggtttt tagttttgag catttgattt atagtcggtt aatagaacat
      121 tacattaatc tttttggcga aaagcaggtg aaggttttcc tgtatgagga ttttaaaaga
      181 aacaacaggg aattcatgga aaatttttgc cagcaatttg gtatggaggt cgattgggat
      241 aaaatcgact ttaacccaac aaacaggggg ttaaggcttt tgagtttgcc gctgcttaag
      301 gtggttaacc atttttacaa gaaaccagtt ggtcgtaagc actttatttg ccatattccc
      361 ggtatgacaa ccgttggacg tggggttata aagtacctta acccaatgcc cgttttcgga
      421 cgctacctga acgaggatga ttttctgagc aaaaaggatt tggagtatat caaaaatttt
      481 tacagccagc acaattgtga gcttacaaag tatttcgaca ggcgggtact ggaaggttat
      541 ggatattatc tatgaaatcg ccaattctac atatagggta tccgaaaaca gccacaaagt
      601 ggtttcagaa tgaattttat cccaacgttt caggggtgta tcctgtaatg agaaaggatt
      661 tgtttgacag attagttttt actgatattt attcatactc acccagtcat ttccggggat
      721 ggataactgg tgaggcgggt agtaaaaggc taattatatg cgatgagatt ttagttgggg
      781 ggttggatat tggttttggg aatggggagt ttgtgttgtt gatgtcgcag aggcttcatg
      841 atacattccc cgattcgcag gtggtaatac tcattcggaa tcagcataca gcattggaat
      901 cggcttacag tcactatgtt atggccggtg gaacatattc gccttcgcga tttttgggga
      961 ttaagccgat gttcataaag ccctatatgg gctataatca atttagcacc aaactttttg
     1021 agtacgataa gctgatagat ctatacatct cactttttgg gaaggataag gttcacattt
     1081 ttctctacga ggattttgct gaaaacccta atcggttcat cgctctgttt tgtaatacac
     1141 ttcaactcga tgctcctcaa ggtcaaacat taaaccgatt aaacgcgcga tattcttaca
     1201 ttgccttgca aatacagcgt ttcctgaatc ggtttgccct tggcaatacc ccatttaaac
     1261 agtactttat taatttcccc agcatttata aaatttcgag gagtttaatc cttgctaccg
     1321 ataagtattg tagttttccc aaatataaat tcgataaaaa agtgcaccat tgggttgaat
     1381 tgcgatttaa ggagtcgaac caatgccttt cgcaatgggt ttcaaaggaa aagttattgc
     1441 gctggggcta tccactttaa cactgtaaac ctttttccta tctttcatac aaaaacattc
     1501 cattttgtga cagaaatcat gagaaaaata tgtaattttg tcagttaacg aatcaaaatg
     1561 gaagttcaaa gcattaagca acgatttgga attattggca actcgccatc tttgaatagg
     1621 gcaattgata ttgcccgtca ggttgctccc actgacctat cggttctaat taccggtgaa
     1681 agcggtacgg gtaaggaggt ctttccgcaa atcattcaca accatagcgc ccgtaagcac
     1741 ggagcatata ttgcggttaa ctgcggtgcc attcccgagg gaacaatcga ttcggagtta
     1801 tttggacacg aaaaaggcgc ttttactggt gcaaccgata gccgtaaggg ctactttgag
     1861 gttgccgatg gcggcaccat ttttctcgat gaggttggtg aattgccctt atccacgcag
     1921 gttcggcttc ttagggtctt ggaaacaggc gagttcattc gtgtaggctc ttcaaaggtt
     1981 caaaagacca acgtgcgtgt ggttgccgca actaacgtaa atcttgttga tgcaatagct
     2041 gaaaacaagt tccgcgagga cttatactac cgcttgaata cggtgcccat ttttattccg
     2101 ccattacgcg agaggcagga tgatattccg ttgcttttcc gtaagtttgc taccgatttt
     2161 gctgaaaagt accggatgcc agcaattgca ctcgacgatg aggctcgcat ggttttaatt
     2221 tcatactatt ggccaggaaa tgtccgccag ctcaagaatg taacagagca aatttcgata
     2281 attgagcaga aaagggtaat cgattccgaa acgcttttac gttaccttcc tgactataac
     2341 gcttcgcgcc tgccaaccct ttaccacaaa aagggtgttg ttgatgagca aacctttgcc
     2401 acagagcggg aaatactcta caagatactt tacgacatgc gcagcgacat gaatgagcta
     2461 aagaagcttg tgttcgatct catgcgcaat ggcgtggttt cgcctgagca cgttcgttca
     2521 tcgccggtat ttcaggattt aggcgttacg catgttgatc caattgtctc atcggcacct
     2581 gctcgtggta cggaaacaaa gtcgtttgtt aaggatttaa atgatattca ggatactgag
     2641 gaaattcagg aggaaaccct ctcgttggag gaaaaggaga aggagttgat agttaaggcg
     2701 cttgaaaagc ataatggtcg acgaaaatgg gctgccatgg agttgggaat ttcagaacgc
     2761 acgctttacc gaaaaattaa ggagtacaat attaaccttt gattatgtcc aggaaaaagt
     2821 tcattatacc gctaatttta gtattcacat tgatctcgca gaatagctgc aaggtgagct
     2881 atagttttac tggggcatca atacccctcg aggctaaaac atatagcgtt cagctcttcc
     2941 agaaccttgc aagcattgta aatccaacct tggccaacta tattaccgag gagttaaaaa
     3001 atagattttt aacccaaacc cgtttaacac cggttgccga ttttggcgat tttgcctttt
     3061 ccggccagat tgtaacctat gatgtttcgc caatggctat tcagggcgat gagatagctg
     3121 ctcaaaaccg gctaaccatt gctgttaagg tgaaatttgt taacgccatt gacccaaaac
     3181 aaagttttga taaaacattc tcgcaatacg ctgtatttag cagtagtcag aatttttccc
     3241 tggtagagca ggcgctggtt caggagatag ttgaaaagct aatcgacgat atttttaatg
     3301 cagcggcatc aaactggt
//
LOCUS       DAOSAL010000134         3261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_18832_length_3261_cov_38.280724, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000134 DAOSAL010000000
VERSION     DAOSAL010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18832_length_3261_cov_38.280724"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2450)
                     /locus_tag="PK049_11405"
     CDS             complement(<1..2450)
                     /locus_tag="PK049_11405"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOK62207.1"
                     /translation="MLKPVIILFLFIPLLGFSQYNPIYKNFTVNDGLAGNETYHVFQD
                     SKGYIWVATSTGVSKFNGYSFQNFDVQSGLVDNIVFEIYEDYKGRIWFIPYSGALSYY
                     EDGKIKPYPYNNKIRQNMPTRSKGPIKMSFYIDSLDNVYLGVKDYGIMHITPEGIFKK
                     FDGINIEAPVVLNEFPNSKILISNPNKPIKHELIINSNTKTTRFPLKDIFDGNNPVYT
                     YAIKPNDSTLYASLEGKVACFVNHKLKWVDRQFKSIIWLSVNDEYLITSDLEGGLNLS
                     PLVNQNGLKYKKILKEFQVTSVIKDSEGSYWVSTLDNGVLYIPDIHIQTITKKDGLSD
                     NRIKTVYQQDGNLYLGYQMGWVDMIKDGKVEKIKMTKTEGNTSFVRYFLGDEANGRIW
                     ICSFGHLGYIQNNRYKVIESKIPVYPRKIVKSKKGNYYWVSTAYGIVKVQDDKVVYDS
                     RDEGFSPLVLGIDEDSKGNVWFVTSNGLWKYSNKLFQFMGDENPLLASPSYALYFNPK
                     DESLWIGTNGAGIVILDKNGKIKQITKDDGLISNSIYAFFAHKNRIWVGTRQGLSIIT
                     FNNGSYSLQNLTTNEGLVSNDITSLYVNDSNAYIGTTSGLSIININRIKPNQTKPNIY
                     ITSIEVADSTYKWTPDGLNIPWDTKYAKISYEGLSYKNMGKALYRYRLLGLDSNWVYT
                     TSNQCLYSVFSPGHYSFEVKVQNSNGIWSNPASLSITVTPPYWKRIWFIILFALTLSG
                     FLYLIYYLRMGELKRRNELINNINLYKQQSLRQQMNPHFIFNTLNSIQYYILEKDTIS
                     SHKYLTKFARLMRYTLDNS"
     gene            complement(2579..>3261)
                     /locus_tag="PK049_11410"
     CDS             complement(2579..>3261)
                     /locus_tag="PK049_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOK62208.1"
                     /translation="LDLKQYFEKKSVWIFGGDGWAYDIGYGGLDHVLSTGEDVNILVL
                     DTEVYSNTGGQASKSTPAAAVAKFAASGKRVRKKDLGMMAMSYGYVYVAQVAMGADHN
                     QYFKAIKEAEAYPGPSLIIAYSPCINHGIRKGMGSTQNEAKMAVKAGYWHLYRYNPML
                     EAEGKNPFQLDSKEPEWNLFQDFLKGEVRYTSLQLSFPQQAEELFKAAEENAKWRYNT
                     YKKMAGNE"
BASE COUNT          897 a          640 c          675 g         1049 t
ORIGIN      
        1 gaattatcga gggtgtagcg catcagccgg gcaaatttgg taaggtactt atgtgaactt
       61 atggtatctt tttccagtat gtagtactga atggaattta gcgtgttgaa gataaaatgg
      121 gggttcatct gctggcgtaa cgattgttgc ttataaagat tgatattgtt gattagctcg
      181 ttacgtcttt tgagctcacc cattcgtaag taataaataa gatataggaa accacttagt
      241 gttaaagcaa aaaggatgat aaaccatatt cttttccagt aaggtggtgt tactgtaata
      301 cttagcgagg cagggttgct ccaaatgccg tttgagtttt gtactttcac ctcaaaggaa
      361 taatgaccgg gcgaaaaaac gctatataag cattggtttg atgtggtgta aacccagttt
      421 gagtcgaggc caagtaggcg gtaccggtat aacgccttgc ccatattctt ataggataag
      481 ccctcatacg aaattttagc atactttgta tcccatggaa tattcaaccc atcgggtgtc
      541 catttatagg tactgtcggc tacctcaatt gaggttatgt atatgttagg tttcgtttgg
      601 ttaggcttaa ttctattgat gtttattatt gacaagccac tagttgttcc aatataggca
      661 tttgaatcat taacgtacag cgatgttata tcgttactaa caaggccttc gtttgtggta
      721 aggttttgaa gagagtaact gccattgttg aaagtaatta ttgacaaccc ctgacgtgtg
      781 ccaacccaaa tcctgttctt gtgggcaaaa aaggcgtata ttgagtttga aattaagcca
      841 tcatccttgg ttatctgctt tatcttgcca tttttatcga gtattacaat accagcgccg
      901 ttagttccaa tccacaagct ttcatctttg gggttaaagt aaagggcata actgggtgat
      961 gccaggagtg gattttcgtc gcccatgaac tggaagagtt tattggaata cttccatagc
     1021 ccattagatg taacaaacca aacatttccc ttgctatctt catcaattcc aagaacaaga
     1081 ggcgaaaaac cttcatctct ggaatcgtac accactttat cgtcctgcac ttttactatt
     1141 ccgtaggcag tagaaaccca gtagtaattg ccctttttac ttttcacaat ttttctgggg
     1201 taaaccggta ttttcgattc aattactttg tacctattgt tctgtatata ccccaaatgt
     1261 ccaaaggagc aaatccaaat cctgccattg gcctcgtcgc ccaggaaata cctgacaaat
     1321 gaggtattcc cctctgtttt tgtcattttg attttttcta ccttcccatc cttgatcatg
     1381 tccacccatc ccatttgata cccaaggtaa agattgccat cctgctggta aacagtttta
     1441 attctattgt cgcttaggcc atcttttttg gtgatggttt gaatgtgtat gtcgggtata
     1501 tacagtacgc cattgtcgag tgtcgaaacc cagtaagagc cttctgagtc cttaattact
     1561 gaagtaacct gaaattcctt aagaatcttt ttgtatttta atccattttg atttaccaat
     1621 ggtgaaagat tcagtccacc ctccagatca ctagtaatga gatactcatc attcacactt
     1681 aaccaaataa tgcttttaaa ttgtctatct acccatttta gtttgtggtt aacaaaacag
     1741 gctacttttc cctctaggga cgcatataat gttgaatcgt ttggctttat tgcgtatgtg
     1801 taaacaggat tatttccatc aaaaatatct ttcaggggga aacgagtggt tttagtgtta
     1861 gagttaataa ttagttcgtg tttaatgggt ttattgggat ttgaaatcaa aattttgcta
     1921 tttgggaatt cgtttagcac aaccggggct tctatgttta taccatcaaa ttttttaaaa
     1981 atcccttcag gagtaatatg cattattcca taatctttta ctccaagata aacgttatca
     2041 agtgaatcaa tgtaaaacga catctttata ggcccttttg accgggttgg catgttttgc
     2101 cttatcttgt tattataggg gtagggtttt atttttccat cttcatagta acttagtgca
     2161 ccggagtagg gaataaacca aatgcggcct ttataatcct catatatttc aaaaacaata
     2221 ttatcgacta aacccgattg aacatcaaaa ttttgaaagc tatacccgtt gaacttgctt
     2281 acgccggtgc tggtggcaac ccagatgtat ccttttgaat cttggaatac gtggtaagtt
     2341 tcatttccgg caagcccatc attaactgta aaatttttat aaattgggtt atactggcta
     2401 aaaccaagta aaggaataaa aagaaataat atgataacgg gctttaacat taaacaaagt
     2461 ttaggactaa tgaatggaac ttacttaatg ctaaagtaat atttttttga taaagtggat
     2521 agtttataaa agaaaaaaaa gccgcatttg agcggctttt tatttacata tggtgagttt
     2581 actcgttacc tgccattttt ttgtaggtgt tgtagcgcca cttggcattc tcctcggcag
     2641 ccttgaagag ttcttcggct tgctgtggga aggagagctg caacgaggtg tagcgaacct
     2701 cacctttaag gaaatcctgg aacaggttcc attcgggctc cttggagtcg agctggaagg
     2761 ggttctttcc ttcggcctca agcatggggt tgtaacggta taggtgccag taaccggctt
     2821 taacggccat cttggcttcg ttttgggtag agcccatacc tttacggatg ccgtggttga
     2881 tacagggcga gtaagcaatg attagcgaag gtccggggta agcttcggct tccttaatgg
     2941 ctttgaagta ctgattatga tcggcaccca tggcaacctg agccacgtaa acgtatccgt
     3001 acgacattgc catcatgcca aggtctttct tacgaacgcg cttacccgat gcggcaaatt
     3061 tagccacagc tgcagccggg gtcgattttg acgcctgtcc accggtattg gagtaaacct
     3121 cggtgtcaag taccaggatg tttacatcct caccggttga aagtacatgg tcaagcccgc
     3181 cgtaaccgat atcgtatgcc caaccgtcgc caccaaagat ccaaaccgat ttcttctcaa
     3241 agtattgctt gagatcgagg a
//
LOCUS       DAOSAL010000135         3188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_19454_length_3188_cov_37.255346, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000135 DAOSAL010000000
VERSION     DAOSAL010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19454_length_3188_cov_37.255346"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="PK049_11415"
     CDS             complement(<1..423)
                     /locus_tag="PK049_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK62209.1"
                     /translation="MKGIIYFTLLTLTVALFSTCQKFEVKPLTKVETGTATPLATSIT
                     VNAKIIDLSGEGNTDHGFCYATQSDPTINDGKVSKGSPKIGDYDASIDGLTPDTRYYV
                     RAYCYSAEGIVYGKTVTITTLDGLPILTTTAVTSITATT"
     gene            complement(449..2836)
                     /locus_tag="PK049_11420"
     CDS             complement(449..2836)
                     /locus_tag="PK049_11420"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOK62210.1"
                     /translation="MKLLKPLLICIAFAICTLSATAQLSSEVRLASVDLRYSNDKVQV
                     PYKIINGKTTDRYVVWVEFFNEKNQLLTAKTVQGDINLVQGNGDKLITWDAKADGFAL
                     NEKLTARVNAKLIPDASTGKAFFYSTLFPGAGDYQFKIGRPYWLYGTLAYGLIGGSIG
                     AYIMSNNAYSKYQNSYLPHTEDPNYKSAQQYRQLSLLMAGAGVAIWLIDYAGITSKSK
                     KYKNLKPQTVIADNGYRLYSATSPAQTINTRGLPPNLFADLEFIDDNRNGILENQENA
                     KLKITLSNQGGGDAMLLEVKVTSDKTDKSMKIDNASQKINVLRPNEKTEIVIPITTDI
                     DLKTATHRLAINVSEHYGYDMEPAYLVLNTYAYQPPQFAISGYEIIDSGIGTMALKSD
                     GKLQVGEQVKVKLVVQNTGQGVANNAVYTISTTDPNIFIDDVNGKLGNVLPGEVKEIY
                     FTLSPNRRVTIKDNLPIFLTITEDIGRGSLKNQQLPIALDQTPPKPNIVEIKSDVESL
                     KKNVARFEYTSNKFRANIANVVNIATVAPSQTKRPNSVGVVIGVSQYKELPPAPYADN
                     DAKIMKNYLEKVLGVEQVIQYTNEQVAGFFFDDIFNPDVGELRKAIVKGESEVFVFYS
                     GHGVPDKDGKNIFLFPSDGKVTRLETQGYNIEKLYENLSRLGAKHVTVILDACFSGAS
                     RSSVKVATENLIGQKGVRVRPKNTWLGDPNFTVITSSTGEETSLGFDETQTGLFTYFL
                     AAGLQGAADANGDKVITLGELKDYVVNNVKETSGKILGTQTPMFHGDDNRVMATY"
BASE COUNT          770 a          690 c          731 g          997 t
ORIGIN      
        1 ggttgtagcg gtaatgcttg tgactgcggt ggtggttagt atgggcaagc catctagcgt
       61 tgtaattgta acggtttttc cgtaaacaat gccctcggct gaatagcagt aggctcttac
      121 gtaataccgg gtgtcaggtg tcagtccgtc aatgctggca tcgtaatctc caatcttagg
      181 gctgcccttg ctaaccttac catcgtttat ggttggatcg ctttgggtag catagcaaaa
      241 tccgtggtcg gtattgccct cgccgctaag gtcaattatt ttggcgttta ccgttatgga
      301 ggttgcaagc ggagttgctg tgcccgtctc tactttggtt agaggcttaa cctcaaactt
      361 ttggcaggtt gagaaaagag caaccgtaag ggttagtaat gtaaagtata ttatcccttt
      421 catatatctt atccttttaa tatctaaatt agtaagtagc catcacccta ttgtcatcgc
      481 catgaaacat tggggtttgc gtacctagta ttttacccga ggtttcctta acgttattca
      541 caacataatc ctttagctcg cccagagtta ttaccttgtc gccattggca tcggcagctc
      601 cctgcaaacc agctgccaga aagtaggtga atagacctgt ttgggtttcg tcgaaaccca
      661 acgaggtttc ctcgccggtg gatgaagtta ttacggtgaa atttgggtcg cccaaccagg
      721 tatttttggg gcgaaccctt acccctttct gacctatgag gttttctgtt gctaccttta
      781 ctgagctacg ggaggcaccg ctaaagcagg catcgagaat cacggttacg tgttttgcgc
      841 ccaatctgct gagattctcg tagagtttct caatgttata gccttgggtt tctaaacggg
      901 taaccttgcc atcgctgggg aaaaggaata tgtttttccc atccttatcg ggaaccccat
      961 gaccggagta gaaaacgaaa acctcgcttt cgcccttcac aatggccttg cgcagctccc
     1021 ctacatcggg gttaaaaata tcatcaaaga agaatccagc tacctgctcg ttagtgtact
     1081 gaatcacctg ctccacacca agcaccttct cgaggtagtt tttcattatt tttgcatcgt
     1141 tatcggcata cggagcgggt ggtagttcct tgtactggga aaccccaatt actactccta
     1201 ccgaatttgg tcgctttgtt tgcgaaggag ccactgtggc aatatttaca acattggcaa
     1261 tgtttgccct aaacttgttt gaggtgtact cgaaacgggc aacattcttc ttcagtgatt
     1321 ccacatccga ctttatttca acaatatttg gcttgggtgg ggtctggtcg agcgcaatgg
     1381 gtaactgctg gttctttaag ctacccctgc cgatatcctc agtaattgtg aggaaaatgg
     1441 gcaggttatc ctttatggta actctacggt tagggcttaa agtaaagtaa atctctttta
     1501 cctcgccagg tagtacattt ccaagtttcc cgttcacatc gtcaatgaat atatttgggt
     1561 cggtggtgct aatggtgtaa acagcgttgt tggcaacacc ttgcccggtg ttttgcacta
     1621 ctaactttac ctttacctgc tcaccaacct gtaacttccc gtccgatttc agggccatcg
     1681 tgccaatgcc ggaatcaata atctcgtagc ccgaaatggc aaactgaggg ggttggtaag
     1741 cgtacgtgtt aagcacaagg tatgcgggct ccatatcgta accgtaatgc tcactcacgt
     1801 taatcgctag cctatgcgtg gctgttttca ggtcgatgtc ggtggtaata ggaattacaa
     1861 tctccgtttt ttcgtttggg cgcaaaacgt taatcttttg gctggcatta tcaatcttca
     1921 tgcttttatc cgttttgtcg gatgttacct ttacctccag tagcatagca tcgccaccac
     1981 cttgattgct aagggtaatt ttcagcttag cattctcctg gttttcaaga ataccgttac
     2041 ggttatcgtc aataaattca aggtcggcaa agaggttggg cggtaaaccg cgtgtattta
     2101 tggtttgcgc cggcgatgtg gccgagtata acctgtatcc gttatcggct atcaccgttt
     2161 ggggttttaa attcttatac tttttcgatt ttgatgtgat accggcgtag tcgatcagcc
     2221 agatagccac ccctgcaccg gccattagca gcgatagctg gcggtactgt tgtgcagatt
     2281 tgtagtttgg gtcttcggtg tgcggtaggt atgaattctg gtacttgcta taagcattgt
     2341 tcgacatgat gtatgctcca atagagccac caattaatcc atacgctaat gtcccgtaaa
     2401 gccagtaggg cctccctatt ttgaactggt agtcaccagc accagggaaa agtgtggaat
     2461 agaaaaatgc ctttccagtg cttgcatcgg ggattagctt tgcgttaacc cgggcggtta
     2521 acttttcgtt aagcgcaaaa ccatcggctt tggcatccca cgtgatcagc ttatcgccat
     2581 tgccttgcac aaggtttata tcgccctgaa ctgtttttgc ggttagtagc tggttctttt
     2641 cgttgaaaaa ctcaacccaa acaacgtacc tatcggttgt tttaccgttt ataattttgt
     2701 agggtacctg aaccttgtcg ttggagtaac gaaggtcaac gctggctaat cgaacttcgg
     2761 atgaaagctg tgcggttgcg cttaaagtac aaattgcaaa agcaatacaa attaaaagtg
     2821 gttttaatag tttcataaat aggtgtttat gcgtttgtag tttattcaaa ttaaaaagtt
     2881 aaattattat aaaaattcaa aatcacaaaa tgagatgttg agttcaatga atttttccac
     2941 atttatcata attaatagtg tctttgagcg aaggcaaacg aaggctcttt tggcagcgaa
     3001 gcggtcgcct gtgcgttcgg gcagccaaat gtgcctgagt gtgccttggg ttttccaaac
     3061 gtttcataaa agtgtgtcgg gcaattaaaa ttgttaatat tcattttttt tcatttacaa
     3121 agagctaaat tatttatttt ctgtgtgtaa accagtttct gtgtgtcgtc atacacttgt
     3181 tgccaacg
//
LOCUS       DAOSAL010000136         3178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_19539_length_3178_cov_43.997118, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000136 DAOSAL010000000
VERSION     DAOSAL010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19539_length_3178_cov_43.997118"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..1499)
                     /locus_tag="PK049_11425"
     CDS             complement(42..1499)
                     /locus_tag="PK049_11425"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOK62211.1"
                     /translation="MVAYATIWLQPRLGTDVALLNGMVREIIANNWVDHSFIESRVEG
                     GMQAFLELKELVEKYTPEVTEKITGVPAQKIKDAARIYATAKTAMVATGMGMSQQVTG
                     THNVFSLLNMILVTGKIGKDRCGIDPPRGQNNVQGATDVGCHPAMYPGYIPVSDVENR
                     RKVAQVWGVPFERLSDRPGLTTVEIIQAAYHGKIKGLYIMGENPMISDPNLNHTEEAL
                     QKLDFLVVQDIFHTETTRFAHVVLPASSWAEKNGTFVNSDRRVLRVRKAVDCPGEARE
                     DWRIIHELAARMGKPMPNYRNEAEIFDELARVTPIMAGISHARLEHHGIQWPCPTPDH
                     PGTPTLYLDKFNTPSGKAKLFPVEHVDQTERVTTEYPFILNSGRILYHYHTGTMSRRN
                     KALTNFTNNPYVIMHPNDASKLGIKHGEKVRITSVRGTLETQVRIADEVLEGELFMPW
                     HYTEAQVNRLTRDELDPYSRIAPFKISAVRVEPAS"
     gene            complement(1411..2766)
                     /locus_tag="PK049_11430"
     CDS             complement(1411..2766)
                     /locus_tag="PK049_11430"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOK62212.1"
                     /translation="MAKVKIYIDGKEVLATEGANLLHQARLNGFNIPGLCYHHKLTPT
                     GACRLCLVKVEGQRGMVASCTLAVKEGMNIVAFDQELEETRRFLLDYLVSEQNTHYDG
                     TYPDELRELMLRYGLDKPENRFFKPVKAHVEFRVDSSSPVLTYDPSKCIKCFRCIKAC
                     DEVQGKNVLSFYERGLNSFIVAGTGVWGDSECDGCGECIQLCPTGAIVEKPNREFIKI
                     ERVEQRVRTTCPYCGVGCQIELLVQDGKIVRSNGVEGVSPNDGRLCVKGRFGYEFVHS
                     PDRLTVPLIRKNGELVESSWDEALTLIAQKFGEIKEKYGSDSLAGYASAKCTNEDNYI
                     FQKFIRTVFGTNNIDYCTRLCHASTVTAMLKSIGDGAGSNSIEDFETTDCLVVTGNNI
                     IETHPITATYVKRGAARGMKIIVIDPKWTPLGCLRHHLATAPFGNRCGSAQRNGQGDY
                     C"
     gene            complement(2769..>3178)
                     /locus_tag="PK049_11435"
     CDS             complement(2769..>3178)
                     /locus_tag="PK049_11435"
                     /inference="COORDINATES: protein motif:HMM:NF022059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOK62213.1"
                     /translation="ALIGGAAGTFASEQHLDVPMGYDKLREVGLTLGSGAVIVMDGTR
                     NLNQTMLSILKFFKHESCGKCIPCRVGTAQLHNTLKESLAQQNFNNNQVYPELIRQAE
                     LIARTSLCPLGQSPILPLKSLWEHILVKQTANK"
BASE COUNT          757 a          807 c          751 g          863 t
ORIGIN      
        1 ggcatggagc aagcgtttac gtatcccctt ggaattttta ctcagctcgc cggctcaacc
       61 ctaacggccg aaatcttgaa tggcgcaatg cgactgtatg ggtcaagttc atcgcgggta
      121 aggcggttaa cctgagcctc ggtgtaatgc cagggcataa agagttctcc ctcaagcacc
      181 tcatcggcga tgcgaacctg ggtttccaga gtacctcgca ccgaggtaat ccttaccttc
      241 tcaccatgtt taatccccaa ttttgatgca tcgttgggat gcataattac atagggattg
      301 ttggtaaagt ttgttagagc cttgtttctg cggctcatgg taccagtatg gtaatggtaa
      361 agaatacggc ccgagttcag tataaatggg tactcggtag taaccctttc ggtttgatcg
      421 acatgctcca ctgggaaaag tttagccttg ccgctgggcg tgttgaattt atcgaggtat
      481 agggtaggtg taccgggatg gtcgggggtg gggcaaggcc actggatacc atgatgttca
      541 agtcgggcat gcgagatgcc agccataatt ggggttaccc tcgccagttc atcgaatatc
      601 tcggcctcgt tgcggtagtt aggcatgggc ttgcccatgc gggcagctag ttcgtggata
      661 atacgccaat cctcacgggc ttcgcccgga caatcgaccg ctttgcgaac ccgcaacaca
      721 cgtctatcgc tgttaacaaa tgttccgttc ttttccgccc acgatgaggc gggcagtaca
      781 acatgtgcaa aacgggtagt ctcagtatgg aaaatatcct gtaccaccaa aaaatcaagc
      841 ttttgaagtg cctcctcggt atggttaagg ttgggatcgc taatcatggg gttttcgccc
      901 ataatgtaaa gcccttttat cttgccatgg taggcggcct gaatgatttc aacggttgta
      961 aggcctggcc tatcggaaag cctttcgaaa ggcacacccc aaacctgagc caccttacgt
     1021 ctattttcaa catccgaaac cggaatatac ccaggataca ttgcaggatg acatcccaca
     1081 tcggtagcgc cctgaacatt attttgtcca cggggtgggt caatgccaca tcgatccttc
     1141 ccaatcttcc cggttacgag tatcatgtta agcaagctaa acacgttatg agtgccagta
     1201 acctgctggc tcatgcccat tcccgtagca accattgcgg ttttggcagt tgcgtatatt
     1261 cgggcagcat ctttgatttt ttgagctgga acgcccgtaa ttttttccgt gacttcaggg
     1321 gtatactttt caaccagctc ctttagctca aggaaggctt gcatcccccc ttccaccctg
     1381 ctctcaataa agctgtggtc aacccagttg ttagcaataa tctccctgac cattccgttg
     1441 agcagagcca catcggttcc caaacggggc tgtagccaga tggtggcgta ggcaaccaag
     1501 tggcgtccat ttgggatcaa tcacaatgat tttcattcct cttgctgcac cccgcttaac
     1561 ataggttgca gtaattgggt gggtttcaat aatattattt ccagttacta ctaaacaatc
     1621 ggtggtttca aaatcttcaa ttgagttgct gccagcccca tcgccaatgg atttgagcat
     1681 ggcagtaacc gttgaggcat ggcaaagcct ggtgcagtag tcaatattat tggttccaaa
     1741 aacagttctg atgaatttct gaaagatgta gttatcctcg ttggtgcatt tggccgaggc
     1801 atagccggcc aacgagtcgg agccatactt ttctttaatc tcgccaaact tttgggctat
     1861 taaggttagc gcctcgtccc aggatgattc aaccaattcg ccatttttgc ggataagggg
     1921 cacagttagc ctatcggggc tatggacaaa ttcatagcca aagcgtccct tcacgcaaag
     1981 cctgccatcg tttggcgaaa cgccttcaac gccattgctg cgaacaattt tgccgtcctg
     2041 gacaagcaac tcaatttggc atcccactcc gcagtagggg caagtagttc gaaccctttg
     2101 ctctaccctt tcgattttga tgaactccct gttgggtttt tccactattg ctccggttgg
     2161 gcaaagctgg atgcactcgc cacagccatc acactcgctg tcgccccaaa cacctgtgcc
     2221 tgcaactata aaggaattca acccccgctc atagaacgac agcacatttt tcccctgcac
     2281 ctcgtcgcag gcctttatgc acctaaagca ctttatgcac tttgaagggt cataggtaag
     2341 cacgggcgaa gaactatcaa ccctaaactc aacatgagcc ttaaccggtt taaagaatcg
     2401 gttttctggc ttatcgagcc cataccttag cattaactcc ctgagctcat cgggataggt
     2461 gccatcgtaa tgggtgtttt gctcgcttac caaatagtcc aaaaggaaac gacgggtttc
     2521 ctccagttcc tggtcaaagg caacaatatt cattccttcc tttacggcaa gggtgcacga
     2581 tgcaaccatg ccccgttgtc cctcaacctt aaccagacat aaacggcagg ccccagttgg
     2641 tgttaattta tggtgatagc ataggccggg tatgttgaaa ccgttaagcc gtgcttggtg
     2701 taacaagttg gcaccctcgg ttgccagcac ctctttccca tcaatatata tctttacttt
     2761 agccatggct acttatttgc agtttgtttt accagtatgt gttcccataa actttttagg
     2821 gggagaattg gtgattggcc aagcggacaa agcgatgtac gcgcaatcag ctcggcttgc
     2881 cttataagtt cagggtaaac ttggttgttg ttgaagtttt gctgcgccaa actctcttta
     2941 agcgtgttgt ggagttgagc cgttcccacc ctacatggaa tacacttacc gcagctctcg
     3001 tgcttaaaga atttaagtat tgatagcatg gtttggttta ggttgcgagt gccatccata
     3061 actattacag cacccgagcc aagggttagt ccaacctcgc gaagcttatc gtagcccatg
     3121 ggcacatcta agtgttgctc cgaggcaaac gtgccagctg cgccgcctat tagcgcag
//
LOCUS       DAOSAL010000137         3053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_20636_length_3053_cov_41.005670, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000137 DAOSAL010000000
VERSION     DAOSAL010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20636_length_3053_cov_41.005670"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="PK049_11440"
     CDS             complement(<1..733)
                     /locus_tag="PK049_11440"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HOK62214.1"
                     /translation="MIETPELHNLLSTAIGAAVRAGYKIMEVYNSDDFQVNLKSDRTP
                     LTLADRLAHEEIKSSLSKTFIPVLSEEGRSIIYEERKGWDYFWVVDPLDGTKEFIKRN
                     GEFTVNIALVYEKYPIAGIVFVPVTGKLYYSINSEGAYMVTSLLSKDDFEISYEDIQA
                     ISQKLPLPSNRDSIVVVESRSHSTPETIQYINSLSERYSNIELLPIGSSLKMCMIAEG
                     KADLYPRLSLSSEWDTAAGQAIVEGA"
     gene            876..2336
                     /locus_tag="PK049_11445"
     CDS             876..2336
                     /locus_tag="PK049_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007664297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HOK62215.1"
                     /translation="MSSNQLKPELVFHYFSEICKIPRPSKKEGKIIAYIIDFAQKHNL
                     EYRKDDAGNILVRKPASAGYENKQAVVLQSHLDMVCEKNSDINHDFNNDPIQTYTDGE
                     WLKAKGTTLGADDGIGIAAQLAILASSDIEHGPLECLFTVDEETGLTGAFEMGSGFFN
                     SKILLNLDSEDEGELFIGCAGGIDTVATFAYEPDRVPSDFVAMKLIVKGLLGGHSGDD
                     IDKHRANSIKLINRFLWKGTQLFDLRLANLDGGNLRNAIPREASAIFTIHKDDKAQLI
                     LSFENIRTEIYNEWRDAEPNLEITIEDSPLPDFVIDEPTHFDLLNSLYACPHGVIAMS
                     RQLKGLVETSTNLASVKFIQDNQILVTTSQRSAVESAKHDISNMVESVFRLANANIQH
                     SDGYPGWAPNTNSQILEITKASYKRLFKVEPVVRAIHAGLECGLFLKKYPELDMISFG
                     PTIKGAHSPDERLHIPSTQKFWDLLLDVLKNIPENK"
     gene            complement(2432..2587)
                     /locus_tag="PK049_11450"
     CDS             complement(2432..2587)
                     /locus_tag="PK049_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOK62216.1"
                     /translation="MYKCRVCGYIYDENIGDPDHGIPAGTPFEELPDNWHCPVCNVTK
                     DYFEEFN"
     gene            2639..>3053
                     /locus_tag="PK049_11455"
     CDS             2639..>3053
                     /locus_tag="PK049_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK62217.1"
                     /translation="METKHQLALVVLILIILITCPTLAFAQKKKEIREAGIKQKIEWR
                     YIYENEKEIKYKEYEAQFDKQGNILMEKNYDEKGNTVKHIEYKYDGEGNEILQTTFNS
                     KGEVLERVETTYSNGFKVEKKVYGPNGKLKSRKVFE"
BASE COUNT         1008 a          641 c          590 g          814 t
ORIGIN      
        1 cagcaccctc aacaattgcc tgaccggcag ccgtgtccca ctcgctggaa agcgaaagac
       61 ggggatataa atccgcttta ccttcggcta tcatgcacat tttaagcgat gagccaatag
      121 gaagtaactc tatgtttgag taacgttctg ataagctatt aatatactga atggtttcag
      181 gtgtgctatg cgacctactc tctacaacca caatgctatc gcgattgctt ggtaaaggca
      241 acttttggga aatagcttga atatcttcat atgagatctc aaaatcatcc tttgaaagta
      301 aagaagtaac catataagca ccttccgaat taatggagta gtaaagcttt ccggtaacag
      361 gcacaaatac tatccctgca attgggtact tttcgtatac aagagcaata tttacagtaa
      421 actccccatt acgtttgata aactcttttg ttccatcaag cggatcaact acccagaaat
      481 agtcccaacc tttacgctct tcgtagatta tactcctacc ttcctcgcta agaactggga
      541 taaaagtctt tgaaagtgaa ctcttaattt cctcatgggc aagtctatcg gctagggtta
      601 acggagtacg atcggattta agatttacct gaaaatcatc agaattatat acctccatta
      661 ttttgtaacc tgctctaacc gcagcaccaa tggcagttga aagcaggttg tgaagttctg
      721 gtgtttctat cattcctgtt gagtgatttt tgttacaaaa ttaaaccatt ttaatatacc
      781 atacaaccgc ttacaaacat gcttttaaac ctcttttatt taaaccatca tatttgaaaa
      841 tgtattatgt ttatgccgaa aaaaaattaa tacccatgag cagtaatcaa ttaaagccag
      901 aacttgtttt ccactacttt agtgaaatat gcaagattcc ccgtccatca aagaaagaag
      961 gtaaaattat tgcctacatc atagattttg cccaaaagca taatctggaa taccggaaag
     1021 atgatgctgg aaacattctg gtaaggaaac ccgcatcagc aggttacgag aacaagcaag
     1081 cggttgtgct gcaaagtcac ctggatatgg tttgtgaaaa gaacagcgat ataaatcatg
     1141 attttaacaa cgaccccata caaacctaca ccgatggtga atggttaaag gctaagggaa
     1201 caacccttgg agccgacgat ggcattggga ttgcagccca attggctatc cttgcctcaa
     1261 gcgatattga gcatgggcca ctagaatgcc ttttcactgt tgatgaggaa accggtctta
     1321 ctggtgcgtt tgaaatggga agcggatttt tcaattcaaa aattctttta aatctcgatt
     1381 ccgaggacga gggcgaactt ttcattggct gcgctggcgg tatcgacaca gtggcaactt
     1441 ttgcatacga acccgatagg gttcctagcg attttgttgc catgaaactg atagtgaaag
     1501 gcttacttgg aggtcactca ggcgacgaca ttgacaagca ccgcgctaac tcaataaagc
     1561 ttattaaccg tttcctctgg aaaggaacac agctatttga tcttagactt gccaaccttg
     1621 atggtggtaa cctacgtaat gccataccac gcgaagcatc ggcaatattc acaatccata
     1681 aggacgataa ggctcaactg attctatcct ttgaaaacat ccgaactgaa atttacaatg
     1741 aatggcgcga tgctgagcca aacctcgaaa tcacaatcga ggactcaccc ctacccgatt
     1801 ttgtaattga cgaacctacc catttcgatt tacttaactc actatacgct tgcccacatg
     1861 gagttatagc catgagtcgc cagctaaaag ggcttgttga aacctctacc aatctagctt
     1921 ccgtaaaatt tattcaggat aaccaaattc tggtaactac cagccaacgc agtgcagtgg
     1981 aatcggcaaa gcatgatatt tcaaacatgg ttgaaagcgt tttccggctt gccaatgcca
     2041 acattcagca ctccgatggt tacccgggat gggcacctaa caccaactct cagatacttg
     2101 aaataacaaa agcctcgtac aagagacttt ttaaggtaga gcctgtagtc agagcaatac
     2161 atgcgggact tgaatgtggc cttttcttaa agaaataccc cgaactggac atgatttcgt
     2221 ttgggccaac cattaagggg gcgcactctc ccgacgaaag acttcatatc ccatcaacac
     2281 aaaagttctg ggatttgctg cttgatgtat taaagaacat ccccgaaaac aagtaataca
     2341 cctaaaaagg gaaagaaccc aaaagggttc tttccctcct tgtactctaa taccaacact
     2401 gtaatgatgg ttcgttcaag acaaacacaa tctagttaaa ctcctcaaag taatccttgg
     2461 ttacattaca tacagggcaa tgccaattgt caggtaactc ctcaaaagga gtacctgctg
     2521 gtattccatg atcaggatcc ccaatatttt cgtcgtatat gtaaccacaa actctacact
     2581 tgtacatagg cgaattattt tttgattaaa ttgcaaaaaa tttatcaaaa ttacgaaaat
     2641 ggaaactaaa caccaactgg cacttgtggt attaatatta ataatattga ttacctgccc
     2701 aactttagca tttgctcaaa agaaaaagga aattcgtgaa gccggcatta aacaaaagat
     2761 tgagtggcgt tatatatacg aaaacgaaaa agagatcaag tacaaggaat acgaggcaca
     2821 atttgacaag caagggaaca tactaatgga aaaaaattac gatgaaaaag gcaatactgt
     2881 aaagcatatt gaatacaaat acgatggtga aggaaacgaa atattgcaga ctaccttcaa
     2941 cagtaaaggc gaagttcttg aaagggtgga aactacatat agcaatggct ttaaagtgga
     3001 aaagaaagta tatgggccta atggaaaact gaaaagtagg aaagtttttg aat
//
LOCUS       DAOSAL010000138         2880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_22380_length_2880_cov_48.283894, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000138 DAOSAL010000000
VERSION     DAOSAL010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22380_length_2880_cov_48.283894"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="PK049_11460"
     CDS             complement(<1..846)
                     /locus_tag="PK049_11460"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HOK62218.1"
                     /translation="MKVDASEISLKEYLNKYFGFTTFKGNQEAIIRNVLAGNDTFVLM
                     PTGGGKSLCYQLPALILDGTAIIISPLIALMKNQVDNMRSFVVEDGVAHFLNSSLNKA
                     QIQKVKDDIMAGTTKMLYVAPESLTKEENVQFLRQVKISFYAIDEAHCISEWGHDFRP
                     EYRRIRPIINEIGQAPLIALTATATPKVQHDIQKNLGMLNATVFKSSFNRPNLYYEVR
                     PKVNAVKEIIKYIKNNPGKSGIIYCLSRKKVEEMAEVLKVNGIKALPYHAGMDASTRS
                     ENQDKF"
     gene            1016..1978
                     /locus_tag="PK049_11465"
     CDS             1016..1978
                     /locus_tag="PK049_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HOK62219.1"
                     /translation="MNSQIKSLAVETIKSEAIAIEKLAEYIDDNFEQTVNEIFNGKGR
                     VIVTGIGKSAIIASKIVATLNSTGTPAIFMHAADAIHGDLGIIQPDDIIICLSKSGNT
                     PEIKVLVPLIKNLGNKVVAIVSNTDSYLAQKADYVLRATVEREACPNNLAPTSSTTAQ
                     LVIGDALAVCLLKLRGFTPADFARFHPGGSLGKKLYMRVSDIFSQNNVPRVHPNDPIP
                     SVIIEISSKRLGATAVLDDNGKLMGIITDGDLRRMLQNNATIEGLTAKDIMSRTPKTI
                     DPHELAINAFHLMEQHKITQLLVMENERYLGVIHIHDILREGIV"
     gene            1995..2783
                     /gene="tatC"
                     /locus_tag="PK049_11470"
     CDS             1995..2783
                     /gene="tatC"
                     /locus_tag="PK049_11470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOK62220.1"
                     /translation="MKSRSEMTILEHLAELRLVLLKVVVAVVLASVVAFLFGDKLLEL
                     LTQVSNPNFITNRWLCKLAGILNSPDLCINSHRLSFTNIELAGQFTLHVRLAFFGGAI
                     LIAPYCVTAIARFILPALKDKERKISIKFLIYSIVLFITGVAFGYFIIAPIAVNFLAN
                     YSLSESIANQITVQSFIRTVTQTVFAMGLSFELPLVVFYLTKWHIIEIEVLKRNRPIA
                     IVVLLTLAAIITPPDVFSMIIVAVPLWLLYEVSIFATKWAKTKD"
BASE COUNT          791 a          604 c          616 g          869 t
ORIGIN      
        1 gaatttatcc tggttctcgg aacgggtgct ggcatccata ccggcatggt aggggagtgc
       61 ctttataccg ttaaccttga gcacctcagc catctcctca acctttttgc ggctaaggca
      121 gtatataata cccgattttc ctgggttatt cttgatgtac ttgattatct ccttaacggc
      181 gttaaccttg gggcgtacct catagtacag gtttggcctg ttgaacgacg atttgaaaac
      241 cgtggcattc agcatgccca ggttcttctg aatatcgtgt tgaacctttg gtgttgctgt
      301 ggcggtaagg gcaattaatg gggcttgacc tatctcgtta ataatgggac gaatacggcg
      361 gtattctggc ctaaaatcgt gcccccattc cgagatacag tgagcctcgt caatggcata
      421 gaacgagatt tttacctgac ggaggaattg aacattctcc tccttggtga gcgactcggg
      481 agccacgtaa agcatcttgg tggttcctgc cattatatcg tcctttacct tctgtatctg
      541 cgccttgttt agcgatgagt taagaaaatg cgccacacca tcttcaacta caaagctacg
      601 catattatct acctgattct tcatcagggc gatgagtggc gatatgatta tggccgtacc
      661 atccagtatg agcgccggaa gctggtagca taacgattta cctccgcctg taggcataag
      721 tacaaaggtg tcgtttccgg caagaacatt cctaatgata gcttcctggt tacctttgaa
      781 ggtggtaaac ccgaaatact tgttcaggta ctcttttaaa gagatttccg atgcatcaac
      841 tttcatttcc ttgcgtttcc cctgggtagg tatttaactt ttgttaatag gtttgtttta
      901 actatcaaat aaaaaaacaa tacttttgct ctaagatata ctattaaatt taaattaagg
      961 tttagtttag cgtcttttaa caaaggatta caaaagtagt aaaaattgac atacaatgaa
     1021 ctcgcaaata aaaagcctgg ctgttgaaac cattaagtcc gaagctattg ctattgagaa
     1081 acttgcagag tacattgacg ataactttga gcaaacggtt aacgaaatat ttaacggaaa
     1141 aggtagggtt attgttaccg gtataggcaa aagcgccata atagcaagca aaatagttgc
     1201 aacccttaac tctactggca caccggccat attcatgcat gctgccgatg ccatccatgg
     1261 cgatttagga attatccaac ctgacgatat tatcatttgc ctgtcaaaga gtggaaacac
     1321 ccctgaaatc aaggtgcttg tacctttaat aaagaattta gggaataagg tagttgccat
     1381 tgtttcaaat actgattcat atttagccca aaaggccgac tatgttttgc gggcaaccgt
     1441 tgagcgcgag gcctgcccca ataaccttgc tcccacatcg agcaccactg ctcagctggt
     1501 aattggcgat gctttggctg tgtgtctttt aaagctacgt ggttttactc ctgccgattt
     1561 tgcccgtttt catcccggag gctcgcttgg gaaaaagctt tacatgaggg tttcagacat
     1621 cttctcgcag aataatgtgc cacgtgttca cccaaatgac ccaatacctt cagttattat
     1681 tgaaatttcg tcgaaaagac ttggtgctac agctgtactg gacgataacg gaaagctcat
     1741 gggtataata accgatggcg acctgcgtcg tatgctccag aacaacgcta ccattgaggg
     1801 gctaaccgcc aaggatatta tgtccagaac gccaaaaact atcgacccgc atgagctggc
     1861 cattaatgcc ttccacctga tggagcagca caaaataacc cagcttttag taatggagaa
     1921 tgaaagatac ctgggagtta ttcacattca cgatatactg cgcgagggta tagtttaacc
     1981 tatcctttat tgccatgaaa agcaggtcgg agatgaccat tcttgagcac ctggcggaac
     2041 tacgattggt tttgcttaaa gtagttgtgg ctgtggtgtt ggcaagtgtg gttgcctttc
     2101 tttttggcga taaacttctt gagctactta cgcaggttag taaccccaac tttatcacaa
     2161 accgatggtt atgcaaactg gctggcatac taaactcacc cgatttgtgc atcaatagcc
     2221 ataggctaag ttttaccaac attgaactag ccgggcaatt taccctgcat gtcaggctgg
     2281 ctttttttgg aggggcaata cttattgcac cttattgtgt aacagccatt gcaaggttta
     2341 ttctacctgc cttaaaggat aaggaacgaa agatttccat aaaattttta atatacagca
     2401 tagttctttt cattactggt gtggcatttg gctactttat tatagcaccc atagccgtta
     2461 atttcctggc taattacagc ttgagcgaat caattgcgaa ccaaataacc gttcaatcgt
     2521 tcattagaac agttacgcaa actgtttttg caatggggtt aagttttgaa ttgcctttag
     2581 tagttttcta cctgactaaa tggcatataa ttgagattga agttttaaag cgaaataggc
     2641 ccatagctat tgttgtgtta cttacattag ctgccattat tacaccgccc gatgtattta
     2701 gtatgataat tgtagctgtg ccgctatggt tgctttacga ggtaagcatt tttgctacaa
     2761 aatgggctaa aactaaggat tagctatgaa acgcctttta acagtatcat ttatagcatt
     2821 tttttgctca aattttgcgc tcggtcaagg tcaaataacc aaaattaggg gcaaggtttt
//
LOCUS       DAOSAL010000139         2760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_23745_length_2760_cov_36.548983, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000139 DAOSAL010000000
VERSION     DAOSAL010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23745_length_2760_cov_36.548983"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="PK049_11475"
     CDS             complement(<1..457)
                     /locus_tag="PK049_11475"
                     /inference="COORDINATES: protein motif:HMM:NF013916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOK62221.1"
                     /translation="MDLSFEDKFNIEVEAAEVEKAINKFKDIAQSRKGDVEILKRCFS
                     MIDLTTLNTTDGYERGKLFAEKVSQFPNQFPGMPNVAAICVYPSLVAAVKENLSAPSV
                     KIASVAAGFPASQTFLEIKLSECEMALEQGADELDVVISVGSFLEGDYFT"
     gene            complement(495..830)
                     /locus_tag="PK049_11480"
     CDS             complement(495..830)
                     /locus_tag="PK049_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005633817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HOK62222.1"
                     /translation="MTQLTLDDAQKLVDNWIKTKGVRYFNELTNMTLLVEEVGELARI
                     MARRYGEQSFKEGETDHDPAPEMADILFVLICLANQTGINLTQALLQSIEKKTKRDSE
                     RHQGNTKLL"
     gene            complement(827..1282)
                     /gene="dtd"
                     /locus_tag="PK049_11485"
     CDS             complement(827..1282)
                     /gene="dtd"
                     /locus_tag="PK049_11485"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOK62223.1"
                     /translation="MRVVIQRVSQASVSVNGSITGSIGNGLLVLAGFEHSDTEEDLEW
                     MVGKIVRLRIFNDAEGIMNLSVMDVNGQILVVSQFTLHAKTKKGNRPSYVQAAPPDIA
                     IPLYEKFIMMLEHATSKRVERGVFGAMMDVSLVNSGPVTIIIDSKNKDL"
     gene            1373..1933
                     /locus_tag="PK049_11490"
     CDS             1373..1933
                     /locus_tag="PK049_11490"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOK62224.1"
                     /translation="MKFLTLLTALLISTTVWSQEFIADASSSTLKWNAKKVTGEHYGK
                     VKLKEGKLVIRDNQIAGGNFVIDMESITCDDLESPEWNKKLIDHLKSDDFFSVGTYRV
                     ALLGNVRSDKFNGDKAKVTTDLTIKGITKPITFEVIKKGNVYTSTITVDRTQYNIRYG
                     SGKFFEGLGDKMIYDEFTIEVKLVVK"
     gene            complement(1937..>2760)
                     /locus_tag="PK049_11495"
     CDS             complement(1937..>2760)
                     /locus_tag="PK049_11495"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOK62225.1"
                     /translation="ITLPCLIFSTFAKIDSNPTLLINGLLVFGFTFVNLAIALFAGTV
                     SARLQGLNPANTTVHSLHTMFGNIVFLGYPLFDALFPGGIGIFYAAAYQLASNSVTFT
                     YGIYRLSAGQAKSGLKSLLNINTFALLIGFTITAFGIKIPAFFIDALSGLGKATSPLS
                     MVYIGALLMGLGIRKSVSHKSIYLLSLNKLILLPVFLGFIYLWIFRFFNLQISSEAFI
                     VLVMQMAMPCQTIIVVMSRRYGGNYQLAAGNLFVSTILSIATLPAIYIYLNFLLK"
BASE COUNT          845 a          566 c          585 g          764 t
ORIGIN      
        1 ccgtaaagta atcaccttca aggaaagaac ctaccgatat caccacatcg agctcatcgg
       61 caccttgctc aagggccatt tcgcattcgg aaagtttaat ttcaaggaat gtttgtgatg
      121 ctggaaaacc tgctgcaacc gaggcaattt ttacgctggg cgcagataga ttttctttta
      181 ctgctgccac aagcgaaggg taaacacaaa tggctgcaac gtttggcatt cccggaaact
      241 ggttagggaa ttggcttacc ttttcggcaa aaagcttgcc tcgctcatag ccatcggttg
      301 tatttagtgt ggtaaggtca atcatgctaa agcagcgctt tagaatctct acgtcgcctt
      361 tacgtgactg agcaatatcc ttgaacttat tgattgcttt ttcaacctcg gcagcttcga
      421 cctcaatgtt aaacttgtct tcgaacgata agtccatagc tttctattat tagattgtga
      481 ttttgttggc aaagttacaa caatttggta ttaccctggt gccgttcgct atcgcgtttg
      541 gttttctttt caatactttg aagaagggct tgggtaaggt taatgccggt ttggttagca
      601 aggcaaatca gcacaaacag tatatcggcc atttcgggag caggatcatg atcggtttcc
      661 ccttccttga acgactgctc gccatatctc ctggccataa ttcgggctaa ctcgcccacc
      721 tcttccacca gcagagtcat gttggtaagc tcattaaagt agcgtacccc tttggtttta
      781 atccaattgt caaccagttt ctgagcatcg tcgagtgtga gctgtgtcat aaatctttat
      841 tttttgagtc gataataatg gttaccgggc cagaatttac caggctaaca tccatcattg
      901 ctccgaacac accacgttca acccgtttac tggttgcgtg ctccagcatc attataaact
      961 tttcatatag cggaatggca atatccgggg gggcagcttg aacgtacgat ggccgattgc
     1021 cttttttggt ttttgcatga agggtaaact ggcttacaac cagtatttga ccgttcacat
     1081 ccataacgct gaggttcatg atgccctcgg catcgttaaa tattcgcagg cggacaattt
     1141 taccaaccat ccattccaaa tcctcttcag tatcggaatg ttcaaaacca gctagtacta
     1201 gaagaccgtt accaattgaa ccggttatgc ttccgttaac cgaaacgcta gcctgactta
     1261 cacgctgtat tacaactctc attggacact aaacaaatat gccccgctaa gttactaaaa
     1321 aacaaattga atattgattt gttattaccg tgtaacttta aacaaaaaga acatgaaatt
     1381 tttaacatta ctaacagcac tcctgattag tactacagtt tggtcacagg agtttattgc
     1441 cgatgcgagt agttccaccc tgaaatggaa tgccaaaaaa gtaactggtg aacattacgg
     1501 caaagtgaag cttaaagagg gcaaattggt tatcagagat aaccagattg ctggcggaaa
     1561 ttttgttatc gatatggaat cgataacatg tgacgattta gagagcccag aatggaataa
     1621 aaagttgatt gaccacctaa aatctgacga tttttttagt gttggaacat accgggttgc
     1681 tctccttgga aatgttaggt cggacaagtt taatggcgat aaggccaaag taacgaccga
     1741 tttgactatc aagggtatta caaagccaat cacctttgag gtaattaaaa agggaaatgt
     1801 gtacacatca acgattacag ttgatcgcac ccagtacaat attcgttatg gatcaggaaa
     1861 attctttgag ggattaggcg ataaaatgat ttacgacgag tttacgattg aagtaaagct
     1921 ggttgtgaaa taaaaatcat ttcagaagaa agttcagata aatataaatg gctggcaatg
     1981 ttgctatact cagtatggtg gagacaaaca gattacccgc agcaagctgg tagtttccac
     2041 cataccttct gctcattacc actattattg tttggcaggg catagccatt tgcatcacaa
     2101 gaacaataaa ggcttcgctc gaaatttgca ggttaaaaaa tcggaatatc cataggtaaa
     2161 taaagcccaa aaaaactggt aataaaatca acttattgag gctaagcagg taaatacttt
     2221 tatggctcac ggatttacga atacccaacc ccataagcaa agccccaatg taaaccattg
     2281 atagggggct ggtagcctta cccaatccac tcagggcatc gataaaaaac gctggaattt
     2341 ttatgccaaa ggcagtaatt gtaaaaccaa ttagtaaggc aaaagtgttg atgttaagca
     2401 aactttttaa ccccgatttt gcttgaccag cacttagcct gtatatcccg taggtgaaag
     2461 taaccgagtt tgaagcaagt tggtatgcag ctgcatagaa tattccaatt cctcctggaa
     2521 atagagcatc aaagagcggg tagcccaaaa acacaatatt cccaaacatg gtgtgaaggc
     2581 tatgaaccgt agtgtttgct gggttcaagc cctggagacg tgccgaaacg gttccagcaa
     2641 aaagagcgat tgcaaggtta acaaaggtaa aaccaaacac tagtaaaccg ttgataagca
     2701 gggtggggtt actatcaatt ttagcaaaag ttgaaaaaat caggcaggga agggtaatgt
//
LOCUS       DAOSAL010000140         2618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_25577_length_2618_cov_47.841982, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000140 DAOSAL010000000
VERSION     DAOSAL010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25577_length_2618_cov_47.841982"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="PK049_11500"
     CDS             <1..288
                     /locus_tag="PK049_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HOK62226.1"
                     /translation="DEGKFSYPGPEPQTKETAIVMMADAVEAASRSLKEYTVQSIDQL
                     VERIIDDQLNSGQYGFADVTLRDISLIKTIFKKKLQNIYHVRLEYPDLSIR"
     gene            complement(291..2456)
                     /locus_tag="PK049_11505"
     CDS             complement(291..2456)
                     /locus_tag="PK049_11505"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOK62227.1"
                     /translation="MSLLIILLISGSTYSQNTQYDRLTFQRDSLFASQLSNQIDSLFY
                     AGQYDSCMLIIDKAIIFFDKLGLTRYKIDALTRRGSIYRINGKFSNAMADFGDVLEYY
                     QRTGNTKGEASTLNHIGAIYRLRGDYPTALDYYFKALANYQKINYLPGISSVLNNIGV
                     VHLYQKIYDKALEYYNKSLSIEETLKSDEGIGTSYLNIGEVYRKKGDLQKAADYYLRA
                     LVYANRTNDLDGIGTIYNELAGINIENGSTDDVLKYLRLAYNTFLSTNSPQRLAECQI
                     NFGKYYQAIGNINQAIKHYSQAFELADKNKLLEIKSDAHRQLSTLFDKQGNTVLAYSH
                     FKKYIETRDSLFNEDNTRKSIQAEFFYKFERQQEEHRIAEAKKEAEYAERLRREKVVK
                     TSLITIISLGAILIGFILFYLNKIKLKNEELKYHQKEILEKNEELQQQQEEILAQRDE
                     IERKNVILEQSQQIIADKNERMISSIEYARTIQNALLPKPEKFSELFSDYFVIYQPKD
                     IVSGDFYWISSDERYSYAAVMDCTGHGVPGAFMSMIGNTLLNKIVIEWGVNQPSKVLE
                     ALNEQLREALKQKEVGNIVLAGIDIAFVTIDKHERKISFSGAARPMLIIQNGEMQLVK
                     GSIRSTGGFQPANTKPFDDACFDLLSPTNIYLFSDGYSDQFSIDKKKFGLQRFTELIY
                     QSHSKPMAAQRDLLIQMMENHLHGADQLDDICVMGLRLA"
BASE COUNT          680 a          520 c          578 g          840 t
ORIGIN      
        1 gacgagggta agttctcata cccagggcca gaaccgcaaa ccaaggaaac agctatcgtg
       61 atgatggccg atgccgttga ggcagcctcg cgtagcctta aggagtatac cgtacaaagt
      121 atcgatcagt tggtcgagag gattatcgat gatcagctta actcagggca gtacggcttt
      181 gccgatgtta cgcttaggga tatttcgctg ataaagacaa tttttaagaa gaagttgcag
      241 aatatttacc atgttcggct tgagtacccc gatttgagta tcaggtagca tcatgctaac
      301 cgaagtccca tcacgcagat atcatcaagt tggtcagcac catgtaaatg gttctccatc
      361 atttggatta gcaggtcgcg ttgtgcagcc atgggtttgc tatgcgattg atagataagt
      421 tcagtaaaac gttgtaagcc aaatttcttt ttatcaatac tgaattgatc ggagtagcca
      481 tcggagaata ggtagatgtt tgttggcgaa agtagatcaa agcaagcatc atcaaagggc
      541 ttggtgtttg ccggttggaa accgccagtt gagcggatgc tgccttttac cagttgcatc
      601 tcgccatttt gtatgattag catgggtcgt gctgcgcctg agaaggagat tttcctttca
      661 tgcttatcga tggttacaaa ggcaatatca atacccgcca aaacaatatt acccacctct
      721 ttttgtttca gggcttcgcg cagctgctcg ttaagcgcct caagaacctt tgatggttgg
      781 ttaacgcccc actcaatcac aatcttgtta agcaaggtgt ttccaatcat cgacatgaaa
      841 gcgccgggca caccatgccc tgtacagtcc ataactgcgg cataggagta acgctcatcg
      901 ctgctaatcc agtaaaagtc gccgctcaca atgtccttgg gttggtatat aacaaagtag
      961 tccgaaaaca attccgaaaa tttttcgggt tttggtagca atgcgttttg gattgttctg
     1021 gcatactcaa tactgctaat catcctttcg ttcttatcgg ctattatctg ttgcgattgt
     1081 tcaaggatta cgttttttcg ctcaatctcg tcgcgttggg ccaaaatttc ttcctgttgc
     1141 tgttggagct cctcgttttt ttcaaggatt tccttttggt ggtatttgag ctcctcgttt
     1201 tttaatttga ttttatttag atagaataga atgaacccaa ttagaatagc gcctagacta
     1261 attattgtga taagtgaagt tttgaccact ttttcgcgcc ttaaacgttc agcgtattcc
     1321 gcttcttttt tggcttcggc aatccggtgt tcttcctgtt ggcgctcaaa cttgtaaaag
     1381 aattccgctt gtatggattt tcgggtgtta tcctcgttaa ataggctatc gcgggtttca
     1441 atatactttt tgaagtggga gtatgccaat acagtattgc cttgcttgtc gaacagtgta
     1501 cttagctgac gatgggcatc gcttttaatt tcaagcagtt tatttttatc ggcaagttca
     1561 aaggcttggc tgtaatgctt tatggcttga ttgatgttgc ctatagcctg gtagtatttc
     1621 ccaaagttta tttggcactc cgcaagtcgt tgaggactgt ttgtacttaa aaaagtattg
     1681 taagctaacc ttaagtactt caaaacatcg tcagtggagc cgttttcgat gttaatgcct
     1741 gccagctcat tgtaaatggt tccaatacca tcaaggtcat ttgtacggtt agcgtaaact
     1801 aaagctctga ggtagtaatc ggcggccttt tgcaaatcgc ctttttttcg gtaaacttcg
     1861 ccaatgttta ggtacgaagt accaatccct tcatcgctct ttagggtttc ctcaatggat
     1921 aacgatttgt tgtagtattc taaagcctta tcgtagattt tttggtacaa atgaacaact
     1981 ccaatattgt tgaggaccga ggaaatgcca ggcaggtagt taattttctg gtaattggcc
     2041 aaagccttaa agtagtagtc aagcgctgtg ggataatcgc ctcttagccg gtaaattgca
     2101 ccaatatggt ttagcgtgga ggcttcgccc tttgtattcc cggttcgttg gtaatactcc
     2161 agcacatcgc caaaatcggc catggcgtta ctaaacttac cattaattct gtaaattgat
     2221 cccctacggg ttaaggcatc aattttatac ctggttaagc ctaacttatc gaaaaagatt
     2281 attgccttgt caattatcag catacagcta tcgtactgtc cggcatagaa taagctatca
     2341 atttggttgg aaagctgcga ggcaaacaag gaatcgcgct gaaaggttaa gcgatcgtac
     2401 tgagtgtttt gggaataagt agaaccgctg attagcaata taatcagtag tgacaaaatg
     2461 ttgaaagttt tagctttagg gtacggcatt acactttcac gactattaat caaatttagc
     2521 catttattta cttttttctc ttattgcaac taataattgg tgtcaatttt tgattaaatt
     2581 gcactatgcg ttaacatagt gttggtttga ggtgataa
//
LOCUS       DAOSAL010000141         2592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_25935_length_2592_cov_48.277493, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000141 DAOSAL010000000
VERSION     DAOSAL010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25935_length_2592_cov_48.277493"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="PK049_11510"
     CDS             complement(<1..664)
                     /locus_tag="PK049_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK62228.1"
                     /translation="MIRAVKAELIKTLGYATFWVLISLHALLFLLVLTIANKATLNFN
                     GVESNILLSKPYTWGTVAWLASWFNLILALLFIILCGNDFLFGTYRRGLLDGFSRNNL
                     FKAKLSLLILIAFYVVVLVTLIALFATPVLSSWENLLTGYGYILVLFIQMLGYFSIAL
                     LIIVLTRNIALTIIVYLLYFALIEPIIRLFLPDSAAQLMPVKIISNLTPMPDFLGIMA
                     KQF"
     gene            complement(676..1566)
                     /locus_tag="PK049_11515"
     CDS             complement(676..1566)
                     /locus_tag="PK049_11515"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOK62229.1"
                     /translation="MHTLLSINKLTKNYGTTEALCNFTLEVVEGQVLGILGPNGSGKT
                     TLLSILMGLRYPTSGHFTWFNTPPNHVPTTRIGALIEVPTFYPYLSLYDNMRISALAK
                     NISYNEIFPAVERVGLTPNLKAKVATFSLGMKQRMAIAQALLGNPKIIVLDEPTNGLD
                     PEGIVEVRNLIKELNAGGTSIIVASHNLDEVQRTCTHVAILKKGHLQAFGGVNELLTS
                     AKVAIVEVRNPENLIDFLNSNPTIEVIERVGNSFKLLVNKSEHQVTLITDFQNHGIAV
                     NSFEIRNLNLEELFITLVRK"
     gene            complement(1585..>2592)
                     /locus_tag="PK049_11520"
     CDS             complement(1585..>2592)
                     /locus_tag="PK049_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RQC domain-containing protein"
                     /protein_id="HOK62230.1"
                     /translation="CDNCLNPKTQFDGQDELQMVLETILAVNQKFKADHIVNILTGNV
                     TAAVKSYKHNQLDTFGEGTDKDPMFWNSIIRQALVMKYIDKDIENYGLLMVTQKGKDF
                     LEKPQEVMFTLDQRYEEGDDDEAIGLSPDKAGGGAADDELLNMLKELRKKMAKKLNLP
                     PFVIFQDPSLEDMAIQYPITIDELQNISGVGAGKARRFGKEFVELIKAYVEEKEIIRP
                     QDMVVKSVANKSLLKVFIIQSIDRKMDFEDIAEAKNLEMDELLTEIEAIVNSGTRINI
                     DYYINKVIDEDKQEEIFDYFRNEAQTESIEEAMAALGDDDITEEEIRLMRIKFMSEMG
                     N"
BASE COUNT          691 a          548 c          596 g          757 t
ORIGIN      
        1 tgaactgctt tgccataatg cccaaaaaat cgggcatggg ggtaagattt gaaattattt
       61 taacaggcat aagctgagct gctgagtcgg gtagaaatag gcgaataata ggttcaatta
      121 atgcaaagta aagcaggtaa actataatgg ttagggcaat atttctggta agtacaatta
      181 ttaagagcgc tatggaaaag tagccaagca tttgaatgaa aagtaccaga atgtaaccgt
      241 agccggttaa aaggttttcc caagagctta gcacaggggt tgcaaaaagc gcaattagcg
      301 ttaccagaac aaccacgtaa aaggcaatta gtattaggag cgatagcttt gccttaaaca
      361 ggttattgcg gctaaagcca tcgagcaaac cgcgccggta tgtgccaaac agaaaatcgt
      421 taccgcacag aataatgaat aaaagggcta aaataaggtt aaaccagctg gctaaccagg
      481 caactgtacc ccatgtgtag ggctttgaaa gcaatatatt ggattctact ccattgaagt
      541 taagtgttgc cttgttagca attgttagaa ccagcaggaa aaggagggca tggagcgaaa
      601 ttagtaccca aaaggttgca taaccaaggg ttttaattag ctccgctttt actgctcgta
      661 tcataggctg tttttttact ttcgaactag tgtaataaat agctcctcaa ggtttaggtt
      721 gcggatttcg aaagagttaa ctgctattcc gtgattttga aaatcggtta taagggttac
      781 ctgatgctcg cttttattta ccagtaattt gaatgaatta cccactcttt caataacctc
      841 aatagtgggg ttgctgttaa ggaaatcaat cagattttca ggatttcgaa cctcaacaat
      901 tgccaccttg gccgatgtga gcagttcgtt gaccccacca aaggcttgca ggtgaccttt
      961 tttaagtatt gccacgtgag tacaggtacg ttgaacctca tcgaggttgt ggcttgcaac
     1021 tatgatggat gtgcctccgg catttagctc tttaattagg ttccgaacct caacaatccc
     1081 ctcggggtct aagccgtttg tgggttcatc aagtacaata atttttgggt tacccagcaa
     1141 ggcttgtgca atagccatgc gttgcttcat tccaagggag aaggtcgcaa cctttgcttt
     1201 taggttgggg gttaggccaa cccgctcaac ggcaggaaaa atctcattat agctgatatt
     1261 cttagccagt gcgctaatac gcatattatc gtaaagggaa aggtaagggt agaatgtggg
     1321 tacctcaata agagccccaa tcctggtggt tggaacatgg tttggtgggg tgttaaacca
     1381 tgtaaagtgg cctgaggttg ggtatcgtaa gcccatcaat atcgatagta gtgtagtttt
     1441 accgctacca ttagggccaa gaattcccag tacctgacct tcaacaacct cgagcgtgaa
     1501 gttacatagc gcttcggttg ttccgtagtt cttggttagt ttgtttattg acagtagtgt
     1561 atgcataccc acttgggaat tgggttagtt acccatttcc gacatgaact tgatacgcat
     1621 caatcggatt tcctcctcgg tgatatcatc atcgccaagg gcagccattg cttcctcaat
     1681 actttcggtc tgagcctcgt tacggaagta gtcgaatatc tcctcctgct tatcctcgtc
     1741 aattactttg ttgatgtagt agtcgatatt gatgcgtgtt ccggaattta caatggcctc
     1801 aatctcagtg agcagttcat ccatctctag gttcttagcc tcagcaatat cctcaaaatc
     1861 cattttgcgg tcgatgcttt ggatgatgaa tactttcagg agcgatttgt ttgccaccga
     1921 tttaacaacc atatcctgag gacggataat ttccttttcc tcaacgtatg ccttaataag
     1981 ctcaacaaat tctttaccga aacgtcgcgc tttacccgct ccaacacccg atatgttctg
     2041 cagttcatca attgtaattg ggtattgaat agccatatcc tccagcgaag ggtcttggaa
     2101 aattacgaat ggcggaaggt ttagcttctt ggccattttc ttgcggagct ctttgagcat
     2161 gttcagcagc tcatcgtcgg cggctccacc accagccttg tcgggcgata gcccaatggc
     2221 ctcgtcgtcg tcgccttcct cgtagcgttg gtcaagggta aacataacct cctgaggttt
     2281 ctcaaggaaa tccttaccct tttgggtaac cattagcaaa ccgtagttct caatatcctt
     2341 atcgatgtac ttcattacca aggcttggcg gattatggag ttccagaaca tggggtcttt
     2401 gtcagtccct tcgccaaagg tgtcgagctg gttatgctta tacgatttaa cggcagcagt
     2461 aacatttccc gtaaggatgt ttactatatg gtcggccttg aacttttggt taacggcaag
     2521 tatggtttcc agaaccattt gaagctcatc ctgcccgtcg aactgggttt tggggttaag
     2581 gcagttatcg ca
//
LOCUS       DAOSAL010000142         2578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_26106_length_2578_cov_51.744352, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000142 DAOSAL010000000
VERSION     DAOSAL010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26106_length_2578_cov_51.744352"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="PK049_11525"
     CDS             <1..687
                     /locus_tag="PK049_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOK62231.1"
                     /translation="RMQSMKTKKHITLDEIGRRIAIGNFRELNLVVKGDVDGSIEALS
                     DSLIKLSTEQVQVNVIHKAVGQISESDILLAAASNAIVIGFQVRPSAGARKLAEKEEI
                     DIRLYSIIYDAINEIKDAIEGMLAPEIKEEITGSAEVLETFKITKVGTVAGCIVREGK
                     ISRSNKVRLIRDGIVIYTGELESLKRFKDDVREVSSGFECGLNIKNFNDIKVGDIVEG
                     FQEVEVKRKL"
     gene            1207..1668
                     /locus_tag="PK049_11530"
     CDS             1207..1668
                     /locus_tag="PK049_11530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOK62232.1"
                     /translation="MEVEKILSKYPRRKDYLLEILHDIQNHSTKNFIPEEAVQRVAEH
                     LNIKKAQVYGVIGYYSMLSIKPRGRYVIRVCASPICNMMGSCSLLDYLKQKLDVELGE
                     TSADGLFTLETSECLGNCAAAPAMMVNQEMYGNLSPGMIDSLLEHYSRQTK"
     gene            1680..>2578
                     /locus_tag="PK049_11535"
     CDS             1680..>2578
                     /locus_tag="PK049_11535"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOK62233.1"
                     /translation="MLKEYRIALRNSGLINPESIDEFIAADGYQALRKALKSEPLGIY
                     EEIYDSGLRGRGGAGFPTGAKEKAAAVICSDCEKYVICNADEGEPGTFKDRIIMETDP
                     HTLIEGLVIAAYSIGAHRGFVYIRGEYELSIQRVQNAIEQAYGRGFLGKNILGSGFDF
                     DLEVRRGAGSYLCGEELTLIESVEGKRGYPRIKPPYPAEKGLWGAPTLINNVETLANF
                     PAIILNGAKWYRSIGTENSPGTKIFTISGDVSKPGFYEFPMGITLRELIYDVAGGIRN
                     GKPFGAALIGGAAGTFASEQHLDV"
BASE COUNT          703 a          537 c          643 g          695 t
ORIGIN      
        1 cgcatgcaaa gcatgaaaac taagaagcac atcaccctcg acgagattgg tcgccgtatt
       61 gccattggaa acttcaggga gcttaacctg gttgtgaagg gcgatgtgga cggttcaata
      121 gaagcgctat ccgactccct gatcaagctc tcaaccgagc aggtacaggt taacgtaata
      181 cataaggcag taggtcagat ttccgaatcg gatatcctgc ttgcagcagc atcaaacgcc
      241 attgtaattg gcttccaggt tcgtccatcg gccggtgccc gtaagctagc cgagaaggag
      301 gaaattgata tacgcctgta ctccatcatc tacgatgcca ttaacgagat taaggatgcc
      361 attgagggca tgcttgcccc tgagattaag gaggagatta ccggatcggc cgaggtactt
      421 gaaacattca agattaccaa ggttggaaca gtggccggat gtattgttcg tgaagggaaa
      481 atctcgcgca gcaataaggt tcgtcttatc cgcgatggta ttgtaatcta caccggcgaa
      541 cttgaatcgc tcaagcgctt caaggacgat gttcgtgagg tttcatcggg ctttgagtgt
      601 ggattaaata tcaagaactt taacgacatc aaggttggcg atattgttga gggattccag
      661 gaggttgagg ttaaacgaaa actctaacca tataaataca taagcagaaa gggttgcgta
      721 gcaacccttt tttcgtttac cttttaaacc aacggaaagt aggtttacct ttatggtatt
      781 cagtaagcag tagtttttgg cttttagtgt ttggtgtttt gttgatttaa gacaaatctg
      841 tgtttgtatt tgctgtcaac taacaacaat caactatcaa cgtaacctgt catcctgagc
      901 gtagcaaagg atatctattt atatatcgtt taataatgtt tcggtggaat ctatcacgat
      961 atatagggac tcaacatgaa aaatctctct aactaaatgg caatgacttt taatttcgat
     1021 tttcgcaggg ccagaaataa gcagaaacaa catgccaatt cattcaaaaa cacgggatcg
     1081 tttaattgat tccccaaatt tttacatccc ttcattcact tccctcccaa gtagtctaat
     1141 tctaaattgc accttactgt tgcttgtagt ttctttttgg cttaacttaa ggcaaaaatc
     1201 ttgagtatgg aggttgaaaa gatactcagc aaatatccta gacgcaagga ttacctgctg
     1261 gaaatcctgc acgatatcca gaaccatagc accaaaaatt tcattcctga ggaggctgtt
     1321 caaagggttg cagagcattt gaatatcaaa aaggcacagg tttatggggt tattgggtac
     1381 tactccatgt taagcattaa accgcgcgga aggtatgtta ttcgtgtatg tgcttcgccc
     1441 atctgcaaca tgatgggtag ctgtagcctt ttggattacc ttaaacaaaa acttgatgtt
     1501 gagctgggcg aaacctccgc cgatggactt tttacccttg aaacatcgga gtgcttaggc
     1561 aactgcgctg cggcaccggc catgatggta aatcaggaaa tgtacggtaa tctttcgccg
     1621 ggaatgatcg atagcctgct ggaacactac tcccggcaaa ccaaataaaa ccgaatgtta
     1681 tgctcaagga ataccgaata gccttacgga attcggggct tattaaccct gaaagtatcg
     1741 atgagttcat tgcagccgat ggctaccagg ctttgcgtaa ggccctgaaa agtgaacccc
     1801 ttggaattta tgaggagatt tacgattccg gtttgcgtgg ccgtggtggt gcgggtttcc
     1861 ctaccggggc aaaggaaaaa gctgctgccg taatctgctc cgattgcgaa aagtatgtga
     1921 tttgcaacgc cgatgagggc gagcctggta cctttaagga tcggattatc atggaaaccg
     1981 acccgcatac gcttatcgaa gggttggtta ttgctgctta cagcattggt gcgcacaggg
     2041 ggttcgttta tatcaggggt gagtatgagt taagtatcca aagggttcag aatgctattg
     2101 agcaggccta tggccgtggg tttctgggca agaatatttt gggtagcgga ttcgatttcg
     2161 acctggaggt tcggcgtggt gcaggctcct acctttgcgg tgaggagcta accctgattg
     2221 agtcagttga ggggaaaagg gggtacccgc gtattaagcc cccttaccct gccgagaagg
     2281 ggctttgggg cgcacctacc ctaatcaaca acgttgaaac ccttgcaaac ttcccggcaa
     2341 ttatcctgaa tggggcaaag tggtaccgtt ccattggaac cgaaaattcg cccggcacaa
     2401 aaatttttac cattagcggc gatgttagta agcccggatt ctatgagttt ccaatgggaa
     2461 tcactttaag ggagttaatc tacgatgtgg caggtggcat taggaatgga aaaccttttg
     2521 gggctgcgct aataggcggc gcagctggca cgtttgcctc ggagcaacac ttagatgt
//
LOCUS       DAOSAL010000143         2500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_27241_length_2500_cov_49.608589, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000143 DAOSAL010000000
VERSION     DAOSAL010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27241_length_2500_cov_49.608589"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(976..1560)
                     /locus_tag="PK049_11540"
     CDS             complement(976..1560)
                     /locus_tag="PK049_11540"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HOK62234.1"
                     /translation="MKRRVLYTATLLLIAMTGWAIPDTSRVALVIIDIQDFYFPGGKS
                     ELVNPIPAAQNAAKVLEHFRNTQQLVVHVGHNATEGQGFYSLVKPAEGEMVFMKNTVN
                     AFVDTGLLEYLQGQQVSKLVICGMQTHMCVEAAVRAAADLGFKVTLIHDACATKDLKW
                     ENETIPAKMVHLSTLATLRSYAKIMSTKEFLGVK"
     gene            complement(1604..1843)
                     /locus_tag="PK049_11545"
     CDS             complement(1604..1843)
                     /locus_tag="PK049_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK62235.1"
                     /translation="MYRGKNSIVVDYVGRIHMNTDPFWFYNLVVFFDEDNQNRLPEGM
                     LSFYHLFVDQLDMKLYEEQIPQSISSSRACFFYMN"
BASE COUNT          749 a          577 c          540 g          634 t
ORIGIN      
        1 ttagccctgg gttttaaccc agggtattga ggtattcaaa ttattttgcg atgcacgcaa
       61 tactttttcc aaagagcaac actgaagcat tgcatcgcaa cgggaaagca tagcaaagag
      121 ccttttacac gttctttctt gtcttgatac aagaaagaac caaagaaaat caaggctgaa
      181 aagcccaacc cgatgggtgc cccgtgggtg gaactgccac gcgatactat tcgccacgcc
      241 tgcagcgtgg ctcatgtggt atcgcttact aggcccaccg ccacgttcca cccctgaccc
      301 attggctggt caggctttta tgcctctgcc tgcttcttcc gctccattct ctctcaggga
      361 taaggtctgg gatgaagcag tttctcacga acaacctaga tgacccctca atatctcccc
      421 tgagagggga gactttagcc ctgcaataag gtctcctaaa tgcttctccc ctctcctgtc
      481 aggagagggg agggggtgag gtcaattcca cgaccaacga acaacgcttc cagccccgta
      541 ggggcgaaac atttgtaacc caatgattac tcacatgtat tgcgatgcac gctatgacat
      601 tccaaagagc aatactgaag cgttgcatcg caaccggaaa gcatggcaaa gagcgattgc
      661 cagagaacta accaaaaggg ataaaaagat taaagttaca aagagatgct tcggctgcgc
      721 tcagcatgat aaagttggtg agggagccaa tggtattgtc attccgagtc ccgattctat
      781 cgggatagac tatgtcgagg agtctcatta taaaagtgat gaaggtacaa agagatgctt
      841 cgacgaattc aacgtgacaa ataaccttaa ttaaccgtca ttgattcaac accactatta
      901 gttagggtta acgcaaataa aaaagggata cggatttaag aatccatatc cctcaaatgc
      961 cttacactaa atattctact taacccccaa aaattccttg gtactcataa tcttggcata
     1021 gctacggagc gtggcaaggg ttgataggtg aaccatttta gcaggaatgg tttcgttttc
     1081 ccacttcaaa tccttggtgg cgcaggcatc gtggattagg gttaccttaa accccaaatc
     1141 ggcggcagcg cgcactgcag cctcaacgca catatgcgtt tgcatgccac aaataaccag
     1201 ctttgaaact tgttgcccct gcaggtattc cagtaaaccc gtatcgacaa aggcgttaac
     1261 ggtgttcttc atgaatacca tttcaccctc ggcgggttta actagcgagt agaacccttg
     1321 cccctcagtg gcattatggc caacatgtac caccagctgt tgggtgtttc ggaaatgctc
     1381 cagtactttg gcggcattct gcgcagccgg tatgggatta accagctccg acttaccgcc
     1441 cggaaagtaa aagtcctgga tatcaattat tacaagggcc acccgcgatg tgtcggggat
     1501 tgcccagcct gtcattgcta taagcaatag agtagcagtg taaagtactc gacgtttcat
     1561 ggctaaatgt tttggtaaat gtagcaagaa tttcaccaac cggttagttc atgtagaaaa
     1621 aacatgcgcg gctactactg atactttggg gtatttgctc ctcgtagagt ttcatgtcca
     1681 gctgatccac aaacaggtgg tagaacgaca gcatgccctc cggcaaccgg ttctgattat
     1741 cctcatcgaa aaatactacg aggttgtaaa accaaaacgg atcggtgttc atgtgaattc
     1801 ggcctacata atcaaccaca atgctgttct tgcccctgta catacccttt ttgaaatgga
     1861 ttaaccaaca attacaacct caagttatgg aataaaaata acattgtcaa cattttaacc
     1921 aataaaaagg gtgatgttta tcattgtact atatactatt gtttggggtc gaaggaaata
     1981 gttataatgt tttagcgatc catatgttaa ggaactctca atatgatgga gttaaaagat
     2041 ggttaataca atttgaaatg ttgaacgaaa tcgaacagct atcaacgaac aggttctcct
     2101 gccattccaa actttccgat ttttcacatc agtaagagga atttattagg agttaaaaga
     2161 agttagaggg atttaaaacg ttaaccgtga accgtgagcc gttaaacggg attgaataaa
     2221 aagcaacgcc ccatgccatt ccgaaatggt gaaggaaccc cacccaaagg ctaaaggcta
     2281 aaaatgggag aaggaagact ccaactttcc ctgatttaag gttcagggaa gaacgatatc
     2341 ctgattgcac ttacacactc caaaaaccaa tcaaagatgg gtatcacagt gttgcactgg
     2401 gttttgcacc atgtagcaca gtgtaaatct gtccactctg cgcgatcagc gttccattta
     2461 aaacactatc aaccaacaac tatcaacgaa caactaaatg
//
LOCUS       DAOSAL010000144         2486 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_27461_length_2486_cov_49.619910, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000144 DAOSAL010000000
VERSION     DAOSAL010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27461_length_2486_cov_49.619910"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            212..820
                     /locus_tag="PK049_11550"
     CDS             212..820
                     /locus_tag="PK049_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005865016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HOK62236.1"
                     /translation="MSKIKLTVLGISYSQTQSGAYALVLSEENGNRRIPIIIGGYEAQ
                     AIAIQLEGLTPPRPLTHDLFLNFARAFGIDLIDVHIYKLEDGVFFSKLHCEDSKKELF
                     IDSRTSDAVALALRFNCPIYTTEEIIEKAGITLDVEEYNSEEPQDESETETPEPTDSF
                     TDQIRRLSLDELNRLLSEAVENEEYEKATKIRDEIKRREKKK"
     gene            complement(889..2157)
                     /locus_tag="PK049_11555"
     CDS             complement(889..2157)
                     /locus_tag="PK049_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010992248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015506 - nucleoside:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HOK62237.1"
                     /translation="MNLKLRLTLMNFLQFFIWGSWLLTIGAYWFQTKQWSGTEFGAIF
                     STMGLASLFMPAIAGIIADRWINAERLYGIFHILGGVMLFIVPMVNNPTTMFWVMLVN
                     MMFYMPTIALSITVAYSSMKSRGMDIIKEYPPIRVWGTIGFIVALWFISLMGFETSST
                     QFYVASMASFILGLYAFTLPQCPPLARGHKKSLVDELGLNAFKLFKSTKMAIFFIFAM
                     LLGASLQLTNAYGDTFLHDFAKLPQFADLVAVKYPAIIMSISQISETLFILTIPFFLK
                     RFGIKNVMLMSMVAWVLRFGLFAVGDPGPGLWMIILSCIIYGMAFDFFNISGSLFVET
                     QSEPSIRASAQGLFMMMTNGFGAFLGSSISGIIIDKFFTIPNAPEGAIQFNWTGIWIT
                     FAAYSLVVAVLFAFLFKHKHNPADVANVQH"
BASE COUNT          780 a          548 c          494 g          664 t
ORIGIN      
        1 atcaccaccc tgctgtacac tgcacactga acactgcaca ctgaacactg cacactattt
       61 ctcgcttcgc tcaggattcc aaaaggaatg cttgtttttg ttaactaatc aacatttaat
      121 tatcaactca aaaaattgtt tgggtttgtg aataaatggt atatttgttt taatttgcaa
      181 atctgtttct acacactgaa catttgttca aatgagtaaa attaagttaa ccgtattagg
      241 gatatcgtat agtcaaaccc aatcaggagc atacgctctg gttctgagcg aggagaatgg
      301 gaaccgacga attcccataa tcataggggg gtacgaggcc caagccatag ccattcagct
      361 cgaagggcta accccgcccc gcccgctaac ccacgaccta ttccttaact ttgcccgtgc
      421 ctttggtatc gatcttattg acgttcacat ctataagctg gaagatggcg ttttcttttc
      481 aaaactgcat tgtgaggata gcaaaaagga actctttatt gactcgcgca cttccgatgc
      541 tgttgccctt gcccttaggt tcaactgccc catttatacc actgaggaga taattgaaaa
      601 ggcaggtatc accctcgatg tagaggagta taattccgaa gaacctcaag atgagagcga
      661 aactgaaaca ccagaaccaa ccgacagctt tactgatcaa atccgcaggt tatcgctcga
      721 tgagctcaac cgcttactta gtgaggctgt tgaaaacgag gagtacgaaa aagcaaccaa
      781 aattcgcgac gagataaaac gtagagaaaa gaaaaagtaa taaaatctcc caaaaacaaa
      841 ggccatgagt ttttcccatg gccttttata tttttacaca cccacctttt agtgctgaac
      901 gttagccaca tcggcagggt tatgcttatg cttgaatagg aaagcgaaca gaacggcaac
      961 cactagcgag taggctgcaa aggttatcca aatgcctgtc cagttgaact gtattgcacc
     1021 ttcaggcgca ttaggtatgg tgaagaactt gtcgattatg ataccgctaa tgctacttcc
     1081 cagaaaagca ccaaaaccgt ttgtcatcat cataaacaaa ccttgcgcac ttgcccttat
     1141 ggatggctca ctttgggttt caacaaacaa cgaacctgat atgttgaaga aatcgaaagc
     1201 cataccgtag attatacatg aaaggataat catccaaaga cccgggcctg gatcgccaac
     1261 tgcaaaaagg ccaaaacgca gcacccatgc taccatgctc atcagcatca catttttaat
     1321 cccaaaacgc ttaaggaaga agggaatggt aagaataaac agagtttcgg agatctgtga
     1381 aatggacatg ataatggcag ggtattttac tgcaaccaga tcagcaaact ggggtagttt
     1441 ggcaaaatcg tgaaggaagg tgtcgccgta ggcgtttgtc agctgcagcg atgcgccaag
     1501 caacatggca aaaatgaaga aaatggccat cttggtgctc ttgaaaagct taaacgcatt
     1561 tagtccaagc tcatcaacca acgacttctt atgaccacga gctaagggcg ggcattgagg
     1621 taaggtaaag gcgtataggc ctaaaatgaa cgatgccatg gaagcaacgt aaaactgggt
     1681 tgatgacgtt tcaaagccca ttaagctgat aaaccataat gccacaataa aaccaattgt
     1741 tccccacacc cttattggcg gatactcttt tatgatatcc attcccctgc ttttcattga
     1801 ggagtaagca accgtgatgg aaagagcaat ggttggcatg taaaacatca tgttaaccag
     1861 cataacccaa aacattgttg tggggttgtt gaccattggg actataaata gcattactcc
     1921 accaagtatg tggaaaattc cgtaaagcct ttcggcattt atccaacggt cggcaatgat
     1981 acctgctatg gcaggcatga acagcgatgc caatcccatt gtggagaaaa tggcgccaaa
     2041 ttctgttccg ctccactgct tggtttgaaa ccagtaagcc cctatggtaa gtaaccatga
     2101 accccaaatg aaaaactgaa ggaaattcat tagggtaagt cgtagtttca aattcataat
     2161 gttcaattta gttgttgatt tggtcggtaa aaatagtatc gtttttttgg ttatactata
     2221 tagtcaacaa ttttttagat acttaaccta aacatttgaa cttttcagag catattttct
     2281 accattttac agaaatcagg gaaatgttga cttactgaaa ccaaaacata gaagttctgc
     2341 agaaaccatt tattatccta ctctataaac cctaatgatg ttttatttac aagccctgaa
     2401 cctactcatt aacccggtga cttatttcag gacgggtcac cctttatcct ttatccttta
     2461 tcctttaccc tttatccttt aacctt
//
LOCUS       DAOSAL010000145         2408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_28724_length_2408_cov_48.629409, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000145 DAOSAL010000000
VERSION     DAOSAL010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28724_length_2408_cov_48.629409"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="PK049_11560"
     CDS             complement(<1..344)
                     /locus_tag="PK049_11560"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOK62238.1"
                     /translation="MAGPKGSKYYDVFLDYSIVLRERNTNQSILTQEQLCLLLNIDEL
                     ESIAASAKKMDISYRKAWELVRRAEEDIGFPLVSKSRGGSDGGKSELTPDGKQLVDAY
                     RQLRNEFNESVKR"
     gene            complement(416..697)
                     /locus_tag="PK049_11565"
     CDS             complement(416..697)
                     /locus_tag="PK049_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HOK62239.1"
                     /translation="MFKINEYFEGKVKSIAFETAEGRATVGVMAQGEYEFGTSTVEYM
                     TVISGQMDVMLPGESTWKTYKEFETYIVPKDVKFKVRVNGDTAYRCLYK"
     gene            1018..>2408
                     /gene="pruA"
                     /locus_tag="PK049_11570"
     CDS             1018..>2408
                     /gene="pruA"
                     /locus_tag="PK049_11570"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HOK62240.1"
                     /translation="MNNAIFKYDLPKNEPVLSYLPCSPEREALEKEIKRLSTGVMDIP
                     LIIGGKEVRTGKLGNVVMPHNHSHVLAQYHIAGEEETQMAIEAALNAHEYWSQVSWVE
                     RLSITLKVAELISKKYRATLNAATMLGQGKNVYQAEIDAACETIDFLRFNAHYISEIY
                     NQQPISETGTINRLEYRPLEGFVFTVSPFNFTAIASNLNMAPVLMGNTVVWKPASTAL
                     LSNYVLMQIYKEAGVPDGVINFIPGKGSAIGNVVFNHPMLAGIHFTGSTVTFNTFWKA
                     VSNNIAKYRSYPKLVGETGGKDFIFIHPSANINQAVIAAVRGAFEYQGQKCSAASRAY
                     IPESLWPEFKRSILAIMHEINMGDPTDYETFVNAVIDEASFDNIMSYIEKAKQSDAAE
                     IIAGGKGDKSKGYFVEPTIIVTTDPHFVTMEEEIFGPVLTIYVYPDEKFEETLELCDK
                     TSPYALTGAIFSS"
BASE COUNT          758 a          529 c          471 g          650 t
ORIGIN      
        1 cgctttacgg attcattaaa ctcgttgcgg agctggcgat aagcatcaac cagttgcttg
       61 ccatcgggag ttagctctga tttgccacca tcactgccac cccgcgactt gctcacaagc
      121 ggaaaaccaa tatcctcctc agctctacga accaactccc atgctttacg gtaactgata
      181 tccatttttt ttgcagatgc tgcaattgat tccagctcat cgatattcaa caataaacaa
      241 agctgctcct gggtaagaat tgattggtta gtgttacgtt cacgtaacac tatggaatag
      301 tcgagaaaaa catcgtagta ttttgatcct ttaggtcctg ccataatccc caatgatatg
      361 caactatttt cctatcggca aatatacaaa agggggatca ttccccctaa tttttttact
      421 tgtaaaggca acgataagcg gtatcgccat ttacgcgaac cttgaatttt acatctttgg
      481 gaacaatgta ggtttcaaac tccttgtagg ttttccaagt tgattcacca ggaagcataa
      541 catccatctg accagaaata acggtcatat actccacggt tgatgtgcca aactcgtatt
      601 caccttgagc cataactcct actgttgccc tgccttcggc agtttcaaag gctatcgatt
      661 taaccttgcc ttcaaagtat tcatttattt taaacatagc ttattttttt cccaaagtta
      721 accagttaaa acatttttag aaatgattta agtcagaaaa ttttgatagt aacataacca
      781 aggatgtaaa cataacactt aatgttcaat aaatgcatta cgacaatttc atattaaaac
      841 aaacaattgt gttacaataa attctattga aattgattga caccttaaaa caaattgcac
      901 ttatcaatgt tattctattt agaatgtttc caaataatag acttttgata aaaatcattt
      961 gaagcaaaac ttgttatgca tatttttaca tatcgataac taaaaacctt aaaaccgatg
     1021 aacaacgcaa ttttcaagta cgatttacct aaaaacgaac cggtgctcag ctacctgcct
     1081 tgctcaccag agcgcgaggc actggaaaaa gagattaaaa ggttatcaac cggtgtaatg
     1141 gatataccac ttataatagg tggcaaggag gttagaacag gaaagttagg caatgtagtt
     1201 atgcctcaca atcacagcca cgtattagcc cagtaccata ttgccggcga ggaagaaacc
     1261 caaatggcca ttgaggctgc tcttaatgcg catgagtact ggtcgcaggt atcgtgggtt
     1321 gagcgtttga gcataacgct caaggtggcc gaactgattt caaaaaagta tcgagccaca
     1381 ttaaatgcag caaccatgct tggacaaggt aagaacgttt accaagctga gattgacgcc
     1441 gcatgcgaaa cgattgactt tttacgattc aacgcccact atatctccga aatttataac
     1501 cagcaaccca tatcagaaac cggaacaata aacaggttgg aatacagacc gctggaagga
     1561 tttgttttta cagtaagtcc attcaacttt acggccatag catcgaacct aaatatggca
     1621 ccggtgctga tggggaatac agtggtttgg aagccagcat caaccgcact actttcgaac
     1681 tacgtactca tgcagattta caaggaagcc ggcgtacccg atggtgtgat caacttcata
     1741 ccaggcaaag gttctgccat tggcaatgtg gtgttcaacc atccaatgct agcaggaatc
     1801 cactttaccg gttcaactgt aactttcaac accttttgga aagcagttag caacaacata
     1861 gccaaatacc gttcgtaccc caaacttgtt ggagaaaccg gtggtaagga tttcattttc
     1921 atacatccaa gcgccaatat taaccaggca gtaatagcag ccgttcgtgg tgcttttgag
     1981 taccaggggc aaaagtgctc agcggcttca agagcatata ttccagagtc gctttggccg
     2041 gaatttaaga gaagcatact tgccattatg cacgagataa acatgggtga tcctactgac
     2101 tacgaaacgt ttgtaaacgc agttattgac gaggcctcgt tcgacaacat aatgagctac
     2161 attgaaaagg caaaacaatc cgatgccgct gaaataattg ccggaggcaa gggcgataaa
     2221 agcaaggggt actttgtgga accaacaatc atagttacaa ccgacccaca ctttgttacc
     2281 atggaagagg aaatatttgg ccctgtgctg accatatatg tttaccccga cgaaaagttt
     2341 gaggaaacgc ttgagctttg cgataaaacc tcgccatatg cacttacggg tgcaatattc
     2401 tcgtccga
//
LOCUS       DAOSAL010000146         2343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_29831_length_2343_cov_48.178759, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000146 DAOSAL010000000
VERSION     DAOSAL010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29831_length_2343_cov_48.178759"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /locus_tag="PK049_11575"
     CDS             <1..1057
                     /locus_tag="PK049_11575"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOK62241.1"
                     /translation="AFDSLWQRGRILGARDFVNPQSDIYKEHYHGTMVLSAMGGYIDG
                     QLIGTAPRANYWLIRTEDAATEQIIEEYNWAAGAEFADSVGADIINSSLGYTTFDVAS
                     QNHTYQDLNGNTAPVTFAANMAASKGMVVVVSAGNDGNAPWHYISVPADSPNVLTVGA
                     VDNKRVKADFSSFGPTADGRIKPDVCAMGKGTVLAIATGTTGTSNGTSFSSPLMAGMV
                     ACLWQAKPSLTAQQIVELVKSSSSNNASPNNSTGYGIPDFAQASTSSITDARNSIELW
                     PNPFTSKINILLGKSFIPANISIFTTNGQLILTMNAFSDGTGHVEINISEELPKGIYL
                     LSIDISGSRYYVTGIKQ"
     gene            1057..>2343
                     /gene="xseA"
                     /locus_tag="PK049_11580"
     CDS             1057..>2343
                     /gene="xseA"
                     /locus_tag="PK049_11580"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOK62242.1"
                     /translation="MVEKQKTYSLKELNLLVKETLSQYLSQAYWIVGEISEIKEHYSG
                     HCYLELIQKDEESDNIVARARATIWSYSYRMLKPYFESTTGRKLSPGLKVMVLAQVSF
                     HEVYGYSLNIIDIEPTFTIGDLELKRRETINRLIADGVFDMNREQTLDLLPNRVAIIS
                     SPQAAGYEDFKKQLLGNKARYRFSIKLFEAVMQGEDAENSIIQALSNIYNEIDRFDLV
                     AIVRGGGASADLSCFDSYSIASHIAQFPLPVITGIGHEKDTTVADLVAFKAVKTPTAL
                     AEYLIDLASEAEQLYLTLLDSLVELSTSYTESSKSQLIVYADQLKSASQLTVFHHKNR
                     LTKTWSSLPVLAKFYMFKSLSQVQGLKEQVKSAAENYVNTPKIAIEKYQKDLKKYTLQ
                     TLSANKQKVDFFGRMIETMNPINTLKKGFTITMIDGK"
BASE COUNT          721 a          527 c          508 g          587 t
ORIGIN      
        1 tgctttcgat agcctttggc aaagaggacg catactgggt gctcgtgatt ttgtgaaccc
       61 acaatcggat atttataagg agcattacca cggcacaatg gtactatcgg ccatgggggg
      121 atatattgac gggcaactaa ttggcacagc cccaagggct aattattggc taattagaac
      181 cgaagatgcg gccaccgagc aaataattga ggaatacaat tgggctgcag gtgcggagtt
      241 tgccgatagt gttggagctg acatcattaa ctcctcgttg ggttatacca cttttgatgt
      301 tgccagccaa aatcacacct atcaggatct taatggcaac acagcccctg tcacatttgc
      361 tgccaacatg gcagcaagca aaggaatggt tgtggttgtg agtgccggaa acgatgggaa
      421 cgccccctgg cactacattt cggttcctgc cgattcgcca aacgttctaa cagtaggtgc
      481 agtagataac aaaagagtaa aggccgattt cagctcattt ggccccacgg ccgatggaag
      541 aataaaaccc gatgtttgcg ccatgggtaa agggactgtt ttggccattg ctaccggaac
      601 aactggaact tccaatggta catctttctc atcacccttg atggctggca tggtggcttg
      661 cctttggcag gccaaaccat cgcttaccgc acaacaaatt gttgagctag ttaaaagttc
      721 atcgtcgaac aatgcatcac ccaacaacag cacgggttac ggaattcctg attttgcgca
      781 ggcatcaact tcaagcatta ctgatgctag aaacagtata gaactttggc caaacccctt
      841 tacttcaaaa attaatatcc ttttaggaaa atcgttcata ccagcaaaca tatcaatttt
      901 cacaaccaac ggtcagctga ttttaactat gaatgctttt agcgatggta ctggtcatgt
      961 tgaaatcaac atttctgagg aattgcccaa agggatttac ctgctctcaa tagatatctc
     1021 aggcagtaga tactacgtta cgggtattaa acagtgatgg tagaaaaaca aaaaacatac
     1081 tcgctcaagg agcttaacct gcttgtaaag gaaaccctaa gccaatactt gtcgcaggca
     1141 tactggattg tgggtgaaat tagcgaaata aaggagcatt actcgggaca ttgctacctt
     1201 gaactcatcc aaaaggacga agaatcggac aacattgtgg cacgtgcaag ggccacaata
     1261 tggtcgtaca gctaccgcat gcttaaaccc tactttgaat ccacaactgg ccgtaagctt
     1321 tctcccggcc ttaaggttat ggtgctggca caggtaagtt ttcatgaggt ttacggctac
     1381 agcctaaata ttattgacat tgaacccaca ttcaccatcg gcgatttgga gctaaaacgg
     1441 cgcgaaacca ttaaccgcct tatagccgac ggtgtatttg acatgaatcg tgaacaaact
     1501 ctggatttac tccctaacag agttgctata atatcgtcgc cgcaggctgc tggatacgaa
     1561 gacttcaaga agcaactgct aggcaacaaa gcccgatacc gtttcagcat caagcttttt
     1621 gaagccgtta tgcaaggcga agacgctgaa aactccataa ttcaagcact ttcaaatatc
     1681 tacaatgaga tagataggtt tgacctggtg gcaattgttc gtgggggtgg cgcctctgcc
     1741 gacctatcgt gctttgattc atacagtatt gcaagccata ttgcacagtt tccactgccc
     1801 gttatcaccg gaattggcca tgaaaaggac acaacagttg ccgatttggt tgctttcaaa
     1861 gcagtaaaaa cacctactgc ccttgcggag tatcttatcg atttagctag cgaggctgag
     1921 cagctttacc ttacactact tgacagccta gtggaactat caacaagcta caccgaaagt
     1981 tcaaaatcgc aactgattgt ttatgccgac cagctcaaat cggcaagtca gcttacggtg
     2041 tttcaccata aaaacaggct gacaaaaacc tggtcatcgc taccagtact tgcaaagttt
     2101 tacatgttta agagcttgtc acaagtacaa ggtttgaaag aacaggtaaa gtctgcagcc
     2161 gaaaactatg ttaatactcc aaaaatcgcc atagaaaaat atcaaaagga cttaaaaaag
     2221 tatacgcttc aaaccctatc ggcaaacaaa caaaaagtag atttttttgg cagaatgata
     2281 gaaacaatga acccaataaa tacgttaaag aaagggttta caataaccat gattgacggg
     2341 aaa
//
LOCUS       DAOSAL010000147         2320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_30256_length_2320_cov_46.127594, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000147 DAOSAL010000000
VERSION     DAOSAL010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30256_length_2320_cov_46.127594"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..2096
                     /locus_tag="PK049_11585"
     CDS             69..2096
                     /locus_tag="PK049_11585"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOK62243.1"
                     /translation="MRKKMFFSNMVKRFTKWSHKGYGVFESFRTVVTIGVLPFSYCLL
                     AMPMATFAQPDSVAVTKNVDIEEVVVNARKKASTYSELTRVVRVVNRDELGQRKATAL
                     GELLEQVAAIDIRQRGANGVQADINFRGGSFDQVMVLLNGINITDPQTGHHNLNIPIN
                     IDAVDRIEILQGPGAREYGVGAFAGAINIITKPDSNSSGQLSFFAGQHGLAKVSGSQN
                     FGTKKLRSFVATSFDRSDGYIENTDFKNLNIYLHTSYTLDKAELNFFAGYQDKGFGAN
                     SFYTPKYPNQYEATKTILGSVSLDKSWKNYALKTAGYYRKHTDRFELFRSNPAVWYTG
                     HNYHATDIYGAKLSVSRFYRWGKTDAGLELRREEILSNKLGTPLTDSVKVWGEDVYYK
                     KGDSRNHLMAFASQSFFFDQFSFSFGGQFNHSNKFGDIWTWGADLSYDLYQHARPFVS
                     VNRSFRLPTFTDLYYQGPTNIGNPNLKAEFATTYESGIKMDRNRLMAEVALFYRQGSD
                     IIDWVKPANETVWTTINYTELNSWGTNINLSLNTKNLNIYTDRITLSYAFTESEKGKS
                     ELDSYYVLDYLRHCLTLSSQHTFKNLFLNWTIRYQDRNGEYLKYIDTNTSIPIAYSNV
                     FLMDVRAGYVFKKVNIYFDVNNLFDNHYVDIANVNQPGRWFGLGINYRFDL"
BASE COUNT          701 a          433 c          508 g          678 t
ORIGIN      
        1 ttacctttgc tggcgttgag tttatatact caacgtaatt aatgtttaac caataaatca
       61 aacaagctat gcgtaaaaaa atgtttttca gtaacatggt aaagcgattc acaaagtggt
      121 cgcacaaggg ttacggtgtt tttgagagtt tcagaacagt tgttaccatc ggagttttac
      181 cattttcgta ttgcctgctt gccatgccca tggcaacctt tgcccagccc gatagcgttg
      241 cggtaacaaa aaatgttgat atcgaagagg tagttgttaa tgctcgcaaa aaggcgagta
      301 cctattcgga gcttaccagg gtagtaaggg ttgttaaccg cgatgagctg gggcaacgta
      361 aggccactgc gcttggtgag ttactcgagc aagtggcggc aattgatatc aggcagcgcg
      421 gagcaaatgg tgttcaggcc gacattaatt tcaggggtgg ctcgttcgac caggtcatgg
      481 tattactgaa tggcatcaac attaccgatc cgcaaaccgg ccatcacaac ctaaacattc
      541 ccattaatat tgatgcggta gatcggatcg aaattctgca aggtccaggg gctagagagt
      601 acggtgttgg ggcttttgct ggtgcaatta acattattac taagcccgat agcaatagta
      661 gcggtcaact ttcctttttt gcaggacaac atggcttagc caaagtgtcg ggttcgcaaa
      721 attttggaac taaaaaatta cgttcctttg ttgcaacatc gttcgatagg agcgatgggt
      781 atattgagaa taccgatttc aaaaatctta atatctacct tcacacatct tatacactcg
      841 ataaagcaga gcttaacttt tttgcaggat atcaggataa gggttttggg gctaatagtt
      901 tttatacgcc taaatatccc aaccagtatg aggctaccaa aaccatctta ggttcagtta
      961 gtcttgataa atcatggaaa aattatgcat tgaaaacagc tggttattac cgtaaacata
     1021 ccgacaggtt tgaacttttc cgaagcaatc cggcagtgtg gtataccggg cataactacc
     1081 atgctaccga tatttacgga gcaaaacttt cggtaagtcg tttttaccgg tggggtaaaa
     1141 ccgatgcagg gcttgaatta aggcgagagg aaatactgag caataagctc ggaacccctt
     1201 taaccgattc cgttaaggtt tggggtgaag atgtttacta caaaaaaggc gattcgcgta
     1261 atcaccttat ggcttttgcc agtcaaagct tcttctttga tcaattctca ttctcgtttg
     1321 gcgggcagtt taatcattcc aataagtttg gcgatatctg gacctggggt gccgatttgt
     1381 catacgacct gtatcagcac gcaaggcctt ttgtttcggt taaccgatcg ttcaggttgc
     1441 caacttttac cgatttatat taccagggcc caaccaatat tggcaatcca aacctaaaag
     1501 cagagtttgc cactacctat gaatcgggta taaaaatgga tcgaaataga ttgatggctg
     1561 aggttgcatt attttacagg cagggtagcg atattattga ctgggtaaaa ccggcaaacg
     1621 aaacggtttg gacaaccatt aactacacag aattaaactc ctggggaacc aacattaatc
     1681 ttagcttgaa tactaaaaat cttaatattt acaccgatag gattactcta tcgtatgcat
     1741 ttaccgaaag cgaaaagggt aaaagcgagc ttgattcata ctacgtgctt gattatttaa
     1801 ggcattgctt aacattgagt tcgcaacata cctttaaaaa ccttttcctg aattggacta
     1861 taaggtatca ggacagaaat ggggagtatt taaaatatat cgatacaaat acatcaattc
     1921 caatagctta ttccaacgtt ttcctaatgg atgtaagggc tggatatgtt ttcaaaaagg
     1981 taaatatcta ttttgatgtg aataatctgt ttgataacca ttatgttgat atagcaaatg
     2041 tgaatcaacc tggcagatgg tttgggctag gtataaatta ccgatttgat ttataacatg
     2101 tttaaaaatt tgcccatttt tgttgaattg gttttatttg caaattcaaa ccaaaaacag
     2161 cttgtaaaat gaagaaaata gtattgattg cttcatcgct acttattgtc agcagtttag
     2221 ctttgacatc ttgcggttcc aaggcgagcg atgccacaaa ccaagaaaaa actgaatgct
     2281 gtggtggtca cagcaaggaa gagggaaaga gttgctgcga
//
LOCUS       DAOSAL010000148         2298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_30682_length_2298_cov_45.361123, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000148 DAOSAL010000000
VERSION     DAOSAL010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30682_length_2298_cov_45.361123"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="PK049_11590"
     CDS             <1..909
                     /locus_tag="PK049_11590"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOK62244.1"
                     /translation="VYVYGVNNSLGTIAQGDSVEVIGTVDEYNNLTEIKSVSYLKKIN
                     SGNTLPTPVDVTPAQAASEAYEGVLVKLNNVECTVADAGHGEFTVSDGTNTINVDDFL
                     YRYTPTQGARYNITGVIDYSFGAFKLLPRSASDVESAQVKYTVTFTVKRSDGTTPVDG
                     ATINVTGQGSVTTDASGVATMQLPDGEYTYTVVKTGFDTNNGSFTVNGADLPVNITLI
                     QTGIPANPIAKLSVYPNPFTDRIYFTGAEVTRVTITSVIGQVVVDRQVENTESVDVSS
                     LDRGIYLVRFYNSKGESVLRKLVKEE"
     gene            complement(1117..2220)
                     /locus_tag="PK049_11595"
     CDS             complement(1117..2220)
                     /locus_tag="PK049_11595"
                     /inference="COORDINATES: protein motif:HMM:NF014653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HOK62245.1"
                     /translation="MAQKFIPFEVIENFMVSVLVKAGIPENDAKIVTDVLIQADKLGF
                     DSHGVNRLKPIYLDRIKDGILNPVTNYQIVKEGPTTAVIDGQNGMGHVIGYNAMKMAI
                     EKAKKFGMGMVAVRNSTHYGFAGYYALMAVKENLIGITGTNARPSIAPTFGVENMLGT
                     NPLTFGMPTDEPFPFLLDCATSITQRGKIELYEREGKILPKGWVIDENGESKTDPKEV
                     LVDLTTGKAALTPLGGIGEETAGYKGYGYATVVEILSSALQQGAYLKMLTGFKDGKKV
                     PYNLGHFFIAIDISAFTEVDDFKRTAGNILRELRASKKMPGQPRIYTAGEKEYEVWKT
                     RKDKGVPFGDELLKEFQGICKEYGLNEFLPSFE"
BASE COUNT          605 a          549 c          479 g          665 t
ORIGIN      
        1 gtgtatgttt acggcgtaaa caactccctt ggaacaattg ctcagggcga tagcgttgaa
       61 gttattggta ctgttgatga gtacaataac cttaccgaga ttaagagcgt aagctacctg
      121 aaaaagatta atagcggtaa cacactgcca acccctgttg atgttactcc tgcacaggct
      181 gccagcgagg cttacgaggg tgttctggtt aagctcaaca acgtagagtg taccgtagcc
      241 gatgcaggac atggcgagtt taccgttagc gatggaacaa acaccattaa cgttgacgac
      301 ttcctgtaca ggtatacccc aacacagggt gcacgttaca acatcaccgg tgtaatagat
      361 tacagctttg gtgcattcaa gcttctgcca cgcagcgcca gcgatgtgga aagcgctcag
      421 gttaagtaca ccgttacctt caccgttaaa cgcagcgatg gtacaacccc tgttgatggt
      481 gctaccatta acgttacagg tcagggcagc gtaactaccg atgccagcgg agtggcaacc
      541 atgcaactcc ccgatggtga atacacctac acggtagtca agacaggttt tgataccaat
      601 aacggtagct ttaccgtaaa cggagccgat ctgcctgtta acatcactct gattcaaaca
      661 ggtatccctg ctaaccccat tgctaagcta agcgtttacc ccaacccgtt cactgatcgt
      721 atctacttca ccggcgctga ggttacccgt gtaaccatca cctcggtaat tggtcaggta
      781 gtagtggatc gtcaggttga gaatactgaa tcggttgatg ttagctcact tgaccgcggt
      841 atttacctgg tacgcttcta caacagcaag ggcgaatcgg tactacgcaa gcttgtaaaa
      901 gaggaataac attcccattg caataaaacg aaagaacccc gctaatgcgg ggtttctttt
      961 tggttgttgg tttatagttg atggtttata gttgatggtt gatggttgat ggttgacggc
     1021 ttaagaaacc accttctatc atgaatctga aatctgaaat tttaaatttt tattctttac
     1081 cctttatcct ttatccttta tcctttatcc tttcctttac tcaaagcttg gcaaaaactc
     1141 gttcaaccca tactccttgc aaattccctg aaactcctta aggagttcat cgccaaacgg
     1201 aacgccttta tccttacggg ttttccaaac ctcatactct ttttcgccag cggtataaat
     1261 acggggttgt cccggcatct ttttcgaggc ccttagctca cgcaggatat taccagcagt
     1321 ccgcttaaaa tcatcaacct cagtaaacgc actaatatca atagcaataa agaaatgacc
     1381 caggttgtat ggaacctttt tgccatcctt aaagccggtg agcattttca ggtaagctcc
     1441 ctgctgtagt gctgacgaaa ggatttccac aacagtggca taaccatatc ccttgtaacc
     1501 tgcggtttcc tcgccaatac ctccaagtgg cgttaatgct gccttgccag ttgttaaatc
     1561 gacgagtact tccttgggat cggttttgct ttcgccattc tcgtcaatca cccaaccctt
     1621 gggcaatatt ttaccttccc tttcgtagag ctcaatttta ccacgctggg taattgatgt
     1681 ggcgcaatcc agaaggaagg ggaaaggctc atcggtaggc attccgaatg ttaatgggtt
     1741 tgtgcctaac atattctcca ccccaaaggt aggtgctatt gatgggcgag cattggttcc
     1801 ggtaatacca atcagattct cctttactgc cattaaagcg taatagcctg caaaaccgta
     1861 gtgagttgag ttacgtacgg caaccattcc cattccaaac tttttggctt tctcaattgc
     1921 cattttcatg gcattgtagc caattacatg ccccatgcca ttctggccat caattacggc
     1981 agtggttggg ccctccttta caatttgata gttggtaacg gggttcagaa taccgtcctt
     2041 tattcgatcg aggtaaatgg gttttagcct gtttaccccg tgtgaatcga acccaagttt
     2101 atcggcctga attaaaacat cggttacaat ttttgcatcg ttttcaggta ttccggcttt
     2161 aaccagtaca ctaaccatga agttttcaat aacttcaaag ggaataaatt tttgcgccat
     2221 aggttttagt ttttttgctt ttcagaatcc aaaattacgg aatttaaatt cctgccacaa
     2281 aacaaaaatt tccttatc
//
LOCUS       DAOSAL010000149         2287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_30875_length_2287_cov_50.629928, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000149 DAOSAL010000000
VERSION     DAOSAL010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30875_length_2287_cov_50.629928"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="PK049_11600"
     CDS             complement(<1..223)
                     /locus_tag="PK049_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007899589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK62246.1"
                     /translation="METYYLFIVGILFLLAASDLMVGVANDAVNFLNSAIGSKVAKRH
                     WILLVASAGVLVGSMFSSGMMEVARKGVFR"
     gene            complement(333..1061)
                     /locus_tag="PK049_11605"
     CDS             complement(333..1061)
                     /locus_tag="PK049_11605"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOK62247.1"
                     /translation="MVKAVIVDDEIHARESLTRMLNVYYPSITVIGKAESIKSAYDLI
                     KNQCPDIVFLDMQLTDGHGFDLLKMFENIPFKVIIISAYQEYAVKAFKFSAVDYLLKP
                     VDPDDLVKAVDIALAQALNDKQQERLNALIENTRNIGKKDKLLALRNSDGTYIVNTSE
                     IVRCESDNNATIFYLVDGNQVRVNRTLKEFDEMLNECGFLRCHQSHLVNQRHIEKISR
                     FPSSLITMNNGDVIPVSFRKRKSI"
     gene            complement(1061..1810)
                     /locus_tag="PK049_11610"
     CDS             complement(1061..1810)
                     /locus_tag="PK049_11610"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOK62248.1"
                     /translation="MFISSTLQVTLLVIHSVVFAIAIIYLITSRKRLKERILRYEQQV
                     LLSQMNPHFVFNSLTAIQSYIFRNEPHTASKYLASFAKLTRLILENSRCELCSLEREI
                     ATLKLYLDLQKLRFEGRFDYTITVAEQIDPDATLIPPMLAQPLIENAIEHGLSGIYWA
                     GKIEIRYTLTKPKQLVIEVEDNGIGIEKSREVQESKGKQYRSLATEITKERIKNLKKL
                     GNSGIKLNITDKSTLSGNTSGTIAQIVIPLK"
     gene            1906..>2287
                     /locus_tag="PK049_11615"
     CDS             1906..>2287
                     /locus_tag="PK049_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK62249.1"
                     /translation="MKRVISIILLGLGVGMMLVHSLVPHHHHNGEVCFVEAAQMCCQH
                     SNGNHHDSPHSHSHDQSCNLLNDLTQAEKFRVIDISQVISNLNVVKDFFANLNRCIDT
                     AVRLELSQRIYISGISKTWQIPIIS"
BASE COUNT          624 a          461 c          474 g          728 t
ORIGIN      
        1 gccggaatac gccctttcgg gcaacttcca tcataccgct tgaaaacatt gaacctacta
       61 atacccctgc cgaagcaacc aacagaatcc aatgcctttt ggcaactttt gaccctatgg
      121 ctgagttaag gaaattaaca gcatcgtttg ccacacctac catcaggtcc gatgcagcca
      181 gtagaaaaag gatacctaca ataaataaat agtatgtttc cattgtatta tagtttgacg
      241 cgcaaaatta ccctttcaaa tgcttggttt cttaaatgcc ctattacttt taggttaacc
      301 caatgttaag ttaatgttaa gtgggttaaa aatcaaattg attttctttt tctaaacgat
      361 actggaatca catcgccatt gttcatggtg attaatgatg aagggaaccg ggaaatcttt
      421 tcaatatgcc gttgattcac caaatgcgat tgatggcagc gtaaaaatcc gcattcgtta
      481 agcatttcgt caaactcctt gagagttcgg tttacccgta cttggttgcc atccacaagg
      541 taaaaaatgg ttgcattgtt atcggattcg catcgaacaa tttctgaagt gttaacaata
      601 taagtaccat cggagttcct gagtgcaagt agcttatctt ttttcccaat attcctggtg
      661 ttttcaataa gggcattgag gcgctcttgt tgcttatcgt tcagggcttg ggctagggca
      721 atatctacag ctttaacaag atcatcgggg tctacgggct ttagaagata gtctacagcg
      781 ctaaacttaa aggctttgac tgcatattcc tgataggcgg aaattataat caccttaaaa
      841 ggtatatttt caaacatctt cagcaggtca aacccatgcc catcggttaa ctgcatgtcg
      901 aggaatacaa tatcggggca ctggtttttt atcaaatcgt aggcgctttt tatgctttca
      961 gctttaccta taacggttat agatgggtaa tatacattaa gcattcgggt tagcgattcc
     1021 cgcgcatgta tctcgtcgtc aactataact gcttttacca ttactttagg ggtattacaa
     1081 tttgagctat agttcccgag gtgttcccac ttagggtcga tttatcggta atgtttagct
     1141 tgattcccga attcccaagc tttttgaggt ttttaatacg ttccttggtt atttcggtag
     1201 ctaacgacct gtactgtttc cctttactct cctgaacttc gcgcgatttc tcaattccta
     1261 taccgttgtc ttccacttca attacaagtt gctttggttt agttagggta tagcgaatct
     1321 ctattttacc tgcccagtaa atgccagaca agccatgctc aatggcattt tcaatgagcg
     1381 gttgcgcaag cataggtggg attagtgttg catcggggtc aatttgctct gcaacggtta
     1441 tggtatagtc gaaccttcct tcaaacctta acttctgtaa gtcgaggtag agcttaaggg
     1501 tggcaatttc tctctctaag ctacacagct cacaacgaga gttttcaagg attaggcggg
     1561 taagctttgc aaagcttgcg agatacttac tggcggtatg gggttcgttg cggaaaatat
     1621 agctttgaat ggctgttaag gagttgaata caaaatgcgg attcatttgc gagagcaaaa
     1681 cttgctgttc gtatcttaaa atccgctctt ttagcctttt tctgctggta ataaggtaga
     1741 ttatggcaat ggcaaacacc acggaatgaa taacaagcag ggttacttgt agagtggaag
     1801 atatgaacat ggtgcacaat tgtttgatgc aaatatatgt tttttggtta aaccattaag
     1861 ccgcaattca atcgaaaaga ataaatttgc acccaattgc ttttcatgaa aagggtcatt
     1921 tcaataatac tattggggct gggtgtgggg atgatgttgg ttcattcgct tgttccgcat
     1981 caccaccaca atggtgaggt ttgctttgtt gaggctgctc agatgtgttg ccagcatagc
     2041 aatggtaacc accacgattc cccccatagc cattcccacg atcagtcatg caaccttttg
     2101 aatgacctta cccaggccga aaagtttagg gttattgata tttcgcaggt aatctcaaat
     2161 cttaacgttg ttaaggattt ttttgctaat ttaaatagat gtatcgatac agccgtaagg
     2221 ctggaacttt cacaaagaat ctacatttct ggtatttcta aaacctggca gattccaatt
     2281 attagct
//
LOCUS       DAOSAL010000150         2144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_33861_length_2144_cov_43.852082, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000150 DAOSAL010000000
VERSION     DAOSAL010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33861_length_2144_cov_43.852082"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="PK049_11620"
     CDS             <1..686
                     /locus_tag="PK049_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOK62250.1"
                     /translation="GAKISGIGSNLLTVEGVESLGGCTHTILPDMIEIGSFIGLAAMT
                     ASDITIRNVSYPNLGIIPDSFRRMGIEFEVIGDDIHIPPQEHYEIDTFIDGSILTIAD
                     APWPGLTPDLLSVFLVVATQAKGSVLIHQKMFESRLFFVDKLIDMGAQIILCDPHRAT
                     VIGHDRNMKLRPTVMTSPDIRAGVALLIAALSAEGKSVIHNIEQIDRGYENIDGRLNA
                     IGADIRRIE"
     gene            784..>2144
                     /locus_tag="PK049_11625"
     CDS             784..>2144
                     /locus_tag="PK049_11625"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOK62251.1"
                     /translation="MRFWTKIHLVLIATVSWAFASQAQLTAPGRVLTLLTQYSNTAKQ
                     DSIFVFYGDIGTLSARHTTGNSASFKWYRYNPLISNPALRFEQFAEETGVAQSNQLNL
                     TEGGYRVVITDITDSTETFTCWLFTDNVTLNRIDIYNSCQFLELNPITTPSSYNVAYD
                     RFVYHDLSRSNQPERNTYGQQYFANVTWQASESRIELASSSALKLVIENPAPLYNSTY
                     TVTIQNPFGRTLTLTTPEVAAIATKADNLIQVDENGVWNDFDANAEYEALLGLRLESK
                     SLNCDSIYWRITTRKITPESIYEITVWRDSSLIAQRNEAFPDKKVMVPGFYKVYHYSV
                     NTLSGCIDSVITDIKVDSSRISADAIPNVFSPNGDGKNDLFRFSDTDESIRSIRSFTI
                     RIYSRSGKLVYTYSGNPRDWEGWDGRTSLGAEAAEGVYYYIIEARGWDDRRFARGPYK
                     GFL"
BASE COUNT          529 a          551 c          491 g          573 t
ORIGIN      
        1 tgggggcaaa aatatcgggt attggttcaa acctgcttac cgttgagggg gttgagtcgc
       61 tgggtggctg cacccatacc attcttcccg atatgattga gattggttcg ttcattggcc
      121 ttgcggccat gactgccagc gatatcacca ttcgtaacgt atcatatcca aaccttggca
      181 ttattcccga ttcgttccgt cgcatgggca ttgagtttga ggttataggc gatgatatcc
      241 atattccacc ccaggagcat tacgagattg atacctttat tgatggttcc atactcacca
      301 ttgccgatgc tccctggccc gggctaactc ccgacctgct cagtgttttc ctggtggttg
      361 ccacccaggc taagggctca gtgctaatcc atcagaaaat gtttgagagc cgactattct
      421 ttgtggataa gctaattgac atgggcgctc aaataatcct ttgcgacccg caccgcgcta
      481 ccgttatagg ccacgatagg aatatgaagc tacgccccac cgttatgacc tcgcccgata
      541 tcagggcagg cgttgccctg cttattgctg ccctttcagc cgaggggaag agcgttattc
      601 acaatattga gcagattgac cgcggatatg agaatattga tgggcgactc aatgccattg
      661 gcgccgatat tcgtaggatt gaatagccaa aatcttcaga aatattctgc aaaactttat
      721 gcataatttt gcatctttac cgtttaataa accaaaacca taccgttaca ctaactattt
      781 ctgatgcgat tttggactaa aatacacctt gtgttaatcg ccactgtgag ttgggccttt
      841 gcctcgcagg cacagctaac tgctccgggg agggttttaa ccttacttac ccagtacagc
      901 aatacggcta aacaggattc aatatttgta ttttatggcg atattggcac gcttagcgct
      961 cgccacacca ccgggaatag tgccagcttt aaatggtata ggtataaccc tctaatctca
     1021 aatcctgcct tgcgctttga gcagtttgcc gaggaaacag gtgttgcgca aagcaaccaa
     1081 ttaaacctga ccgagggtgg ctacagagtg gtaatcactg atattaccga ttcaactgaa
     1141 acctttacct gctggctttt taccgataat gttacgctca accggataga catatacaac
     1201 agctgccagt tccttgagct taacccaatc acaactccct cgtcgtacaa cgttgcctac
     1261 gaccggtttg tctaccatga cctttcgcgc tcaaatcagc ctgaacggaa tacttacgga
     1321 cagcagtact ttgctaatgt tacctggcag gcatcggaga gccgtattga gctggcatcg
     1381 tcctcagcat taaagttggt tattgaaaat cctgcacctc tttataattc cacctatacc
     1441 gttaccatcc aaaacccgtt tggccgaacc ctaaccctta caaccccgga ggttgctgcc
     1501 attgcaacca aggccgataa tctgattcag gttgacgaaa atggagtatg gaacgatttt
     1561 gatgcgaatg ccgagtatga ggctctgctt ggcctgaggc ttgaaagcaa aagcctgaac
     1621 tgcgattcca tttactggcg cattacaacc cgaaaaatta cccctgaaag catctatgaa
     1681 attactgttt ggcgcgatag ctccttgatt gcacagcgaa acgaggcctt ccccgataaa
     1741 aaggtaatgg ttcccggatt ctacaaggtt taccattact cggtaaatac cctctcgggg
     1801 tgtatcgact cggttattac cgatattaag gttgattcct cgcgcataag cgccgatgcc
     1861 atccccaatg ttttttcgcc caatggcgat ggcaagaacg accttttccg cttttccgac
     1921 accgatgaga gcatccgctc catccgttcg tttaccatcc gtatatatag ccgcagcggt
     1981 aagttggtgt acacctatag tggcaatccc cgcgattggg agggctggga tggtcgcacc
     2041 agcctaggag ccgaggccgc cgagggtgtt tactactaca ttatcgaggc ccgtggctgg
     2101 gacgatcgtc gttttgcccg tggcccctac aaggggttcc tgca
//
LOCUS       DAOSAL010000151         2137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_34042_length_2137_cov_34.202690, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000151 DAOSAL010000000
VERSION     DAOSAL010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34042_length_2137_cov_34.202690"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..776)
                     /locus_tag="PK049_11630"
     CDS             complement(177..776)
                     /locus_tag="PK049_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK62252.1"
                     /translation="MKHKLSPIIITVLTTVMLVNTLSAQQKRTNWLQEKNEYTGRWRV
                     GVGANIVEPTAVDVQFYRLSKICTSQFAINKKIAIGIWAGMEGLLLGSLIERQNNKTW
                     EKGSIRYGIDAKFYLPIALNPYFGFGIEGGNRTLNNESTFSPDFVARIGLEQKIAGIK
                     LSTRSALNITFFIDGKLNKCLNVDFVYIMPSLGLRFHWI"
     gene            1181..>2137
                     /locus_tag="PK049_11635"
     CDS             1181..>2137
                     /locus_tag="PK049_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HOK62253.1"
                     /translation="MNDSEILMNPNELVRFLKKPASEFTKNDIIRFCEANNIQMVNFR
                     YVAEDGKLKTLNFVISSKDHLDSVLSAGERVDGSSLFSFIEAGSSDLYVIPRYRTAFV
                     NPFSDVPTIDILCSFYDSTGKPLESAPENVLRKAHDLFKSETGMTFKALAELEYYVIS
                     EREDFYPGVDQKGYHASEPFAKWENLRREALIYIASAGGKVKYGHSEVGCFMTDEYLY
                     EQHEIEFLPMPAEEAVEQLIIAKWIVRMLGYRYGVDISFAPKITVGKAGSGLHFHMMV
                     EKDGKNMMVENGKLSNTAKKMVAGILDAADALTAFGNTIPISY"
BASE COUNT          631 a          454 c          447 g          605 t
ORIGIN      
        1 gctcagcatg acaaagagcg taaggatgca aggggtattg tcattccgag ctagtcgagg
       61 aatctcatta aagggatgaa aggacaaaga gatgcttcgg cagcgctcag catgacaaat
      121 atccctatct taacgatatt aaataaattt tactttccaa acatttttct aatgcactaa
      181 atccagtgaa aacgcaatcc aaggcttggc atgatgtaga caaaatcgac attcaggcat
      241 ttatttagct tcccatctat aaagaatgtg atgttcagtg ccgaccgtgt ggatagttta
      301 attccggcaa tcttttgttc aagcccaatg cgcgcaacaa aatcgggcga aaaagtgctc
      361 tcgttattta aagttctatt tccaccttca ataccaaaac caaagtacgg gttaagggca
      421 atgggcaggt aaaacttggc atctattccg tagcgtatgc tccctttctc ccaggtcttg
      481 ttattctgcc tttctatcag ggagcctaat agcaaacctt ccatgccagc ccaaatacca
      541 atggcaattt tcttgttaat ggcaaattgg cttgtgcaga tcttgctcaa acggtaaaac
      601 tgaacatcca ccgctgttgg ctcaacaata ttggcaccaa caccaacacg ccagcggcct
      661 gtgtactcgt ttttctcctg caaccagttt gttctcttct gctgggcgga caacgtattt
      721 accagcatta ccgtagttag aacagttatg attatgggtg aaagtttatg tttcatgcag
      781 tgacagattt tttccacctt tactagttat acttaaaagt taacagtgca atttaacgaa
      841 attattgtcc attgctgtga ttttgcctgc aattaaaaat tagatggtat ttgcctaagc
      901 tagctgcaat catttaatga catctgcgaa ccgttggaaa gatactcaaa catatttaat
      961 cgcctaacca attcaaaaat tgactcgaac tcacattcgg cagaaatata tcgagataaa
     1021 gcctttggtt ttagaaaaaa tgtctatgca ttttgtctat tattgaaaat acttctactt
     1081 taagggaaaa tgctataaaa tgatgttgat aaacatagcc gcaaacgaat gcgatgtcga
     1141 ttgattttgt agctttggca aaataaaaaa ttaggaacac atgaacgatt ctgaaattct
     1201 gatgaaccct aatgagctgg ttcgctttct gaagaaaccc gcatcggagt ttaccaagaa
     1261 cgatattatt cgcttttgcg aggctaacaa cattcaaatg gttaacttcc gctatgtggc
     1321 cgaggatggc aagctaaaaa ccctgaactt tgtaatatca tcaaaggatc acctcgattc
     1381 cgttctatcg gccggagaga gggttgatgg tagtagtttg ttctcgttca ttgaggccgg
     1441 aagtagcgac ctttacgtta taccacgtta ccgtaccgct tttgtgaatc ccttttccga
     1501 tgttcctaca attgacattc tttgctcatt ttacgatagc accggcaaac cccttgaaag
     1561 cgctcctgaa aatgtgctac gcaaagctca cgatttattc aagagcgaaa cgggcatgac
     1621 ctttaaggca cttgcagaac tggagtacta cgtgattagc gaacgcgaag atttttaccc
     1681 gggtgtcgat caaaagggtt accacgcctc ggaaccattt gcaaagtggg aaaacctacg
     1741 tcgcgaggct cttatttaca ttgccagtgc tggaggcaag gttaagtatg gccactcaga
     1801 ggttggttgc ttcatgaccg acgaatatct atacgagcag cacgagattg aattccttcc
     1861 catgcctgct gaggaggctg ttgaacagct gattattgcc aagtggattg ttcgaatgct
     1921 tggctatcgt tatggagtag atattagctt tgccccaaaa atcactgtag gaaaggcagg
     1981 aagcggctta catttccaca tgatggtaga aaaagatggc aaaaacatga tggttgaaaa
     2041 cggtaagctt agcaataccg ccaagaaaat ggttgccgga atcctcgatg cagccgatgc
     2101 ccttaccgcc tttggtaata ccatacccat atcgtac
//
LOCUS       DAOSAL010000152         2128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_34273_length_2128_cov_48.049204, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000152 DAOSAL010000000
VERSION     DAOSAL010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34273_length_2128_cov_48.049204"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1263)
                     /gene="ffh"
                     /locus_tag="PK049_11640"
     CDS             complement(<1..1263)
                     /gene="ffh"
                     /locus_tag="PK049_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOK62254.1"
                     /translation="MFENLTDRLERSFKILKGEGKITEINVAETVKEIRKALIEADVN
                     YKVAKTFTDEVKRKALGMNVLNAIKPGQLMVKVVHDELVSLMGGQATDINLAGNPTVI
                     LIAGLQGSGKTTFSAKLANHLKNKKGKSPLLVAGDVYRPAAIEQLKVLGQQINVPVFT
                     IPGSNDPIGIAREAVRLAKNNNQNVVIIDTAGRLAIDEEMMKEVEAIKKAVSPNEILF
                     VVDSMTGQDAVNTAKEFNDRLNFDGVVLTKLDGDTRGGAALSIRAVVDKPIKFVSTGE
                     KLDALDIFHPDRMADRILGMGDIVSLVEKAQEQYDAEQAKALHKKILKDQFNFNDFLE
                     QINQIKKMGNLKDLAGMIPGVGKALKDIDIDDDAFKNIEAIIKSMTPEERENPSLMNG
                     SRRKRIAEGSGTSIVEVNRLLKQFEDMRK"
     gene            complement(1319..1966)
                     /locus_tag="PK049_11645"
     CDS             complement(1319..1966)
                     /locus_tag="PK049_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK62255.1"
                     /translation="MQLNKFCLSAFVILVTTFNVKAQEFEPAKDFGSEFGITGMFGKE
                     GIDGNNSAYVGFTGAYFYDLKNGLRVGINRYYNFSGAEYAYTLPIYFAHRTKSSRSNE
                     PFVFDTFGEFLLQLFTHIIPGRAEFNAGPVIGYFKSTNQTISNGYKLTNPVYLAANAK
                     LRLTFQIWRFSLGGNFGASYVPSKNFYLVSPDPVFNGNRTSWSLEIGAFLSFSFD"
     gene            complement(1988..>2128)
                     /locus_tag="PK049_11650"
     CDS             complement(1988..>2128)
                     /locus_tag="PK049_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005850079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HOK62256.1"
                     /translation="QFYHDFTILKEPNEGIRLLRLSLSEQVADIIKRAMWLLGIDVPE
                     RM"
BASE COUNT          585 a          473 c          438 g          632 t
ORIGIN      
        1 cttacgcata tcctcaaatt gtttcagcaa tcggttaacc tcaacgattg atgttccgct
       61 accctccgct atacgcttac ggcgactgcc attcatcagc gatgggtttt cgcgttcttc
      121 aggggtcatc gatttgatga tggcttcgat gtttttaaaa gcatcatcat caatatcaat
      181 atcctttaag gccttgccaa ccccgggaat cataccggca aggtctttta ggttacccat
      241 ctttttaatc tggttaatct gttcaagaaa atcgttaaag ttgaactgat ccttaagtat
      301 ctttttgtgg agggctttag cctgttcggc atcgtattgt tcttgcgctt tctcaaccag
      361 cgagacaata tcgcccattc caaggatacg atcggccatt cgatcggggt ggaagatatc
      421 gagagcatca agtttttcgc cggtgctaac gaattttatg ggtttatcaa caacggcacg
      481 tatggagagg gctgcaccgc cacgggtatc accatcgagc ttggtaagca caacgccgtc
      541 aaagttgagc ctatcgttaa attcctttgc ggtgtttacc gcatcctggc cggtcatgga
      601 gtcgactacg aaaaggattt cattgggtga aacagccttt ttgatggctt caacctcttt
      661 catcatctcc tcgtcgatag ccaaacgccc agcagtatca attattacaa cgttctgatt
      721 gttgttcttt gccagcctga cagcttccct ggcaattcca ataggatcgt tgctgccggg
      781 tatggtaaat actggaacat taatttgttg acctaaaacc ttaagctgtt caatagcagc
      841 gggtcggtaa acgtcgcctg caaccagcag agggcttttc ccctttttgt tttttaggtg
      901 gttggcaagt tttgccgaga aagttgtttt acctgaaccc tgtaaacctg caattaggat
      961 aactgttgga ttacctgcaa ggttaatatc cgtggcttgt ccgcccataa ggctaacaag
     1021 ttcatcgtgt acaaccttaa ccatcagttg cccaggctta atggcattta gtacattcat
     1081 gccaagtgcc ttacgcttta cctcatcggt aaatgttttg gcaaccttgt agttaacatc
     1141 ggcctcaata agtgccttgc ggatttcctt tacggtttcg gcaacattaa tctcggtgat
     1201 tttaccttca ccttttagta ttttaaaaga gcgctcaagc ctatcggtta aattctcaaa
     1261 catatcggta tgctcttatt tcaaattatt atgcaaactt aataaaaata tgattttttt
     1321 aatcgaagga gaatgataga aaggcaccaa tctcaagcga ccaactggtc ctattcccat
     1381 taaagacggg gtcgggtgaa accaagtaaa aattttttga tggtacgtag ctggcgccga
     1441 agtttccccc aagactaaac cgccaaatct gaaaggtaag cctaagcttt gcattggctg
     1501 caaggtaaac cgggttggtg agtttatacc cattactaat ggtttggttg gttgatttaa
     1561 aataaccaat caccggtcca gcattgaact ctgcacggcc gggaattata tgggtaaaaa
     1621 gttgtagtaa gaactcgcca aaagtatcaa acacaaaggg ttcattactt ctactggatt
     1681 tggttcgatg agcaaagtag atagggaggg tataagcgta ttcggctccg ctaaaattgt
     1741 agtaccgatt tattccaact cgaagtccgt tttttaaatc gtagaagtaa gctcctgtaa
     1801 atccaacgta agcagagttg ttcccatcta ttccttcctt cccaaacatg cctgttattc
     1861 caaactcaga gccaaaatcc ttagcgggct caaattcctg tgccttaacg ttaaaagttg
     1921 taaccaaaat tacaaaggca gaaagacaaa acttattcaa ttgcatacta aaaaaaaaat
     1981 caagggatta cattcgttca ggcacatcga taccaagtaa ccacatggcc cgtttgatta
     2041 tatcggctac ctgttccgag agcgataacc taagcaacct tataccctcg ttaggttctt
     2101 taagaatggt gaaatcatgg tagaactg
//
LOCUS       DAOSAL010000153         2083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_35320_length_2083_cov_51.727318, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000153 DAOSAL010000000
VERSION     DAOSAL010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35320_length_2083_cov_51.727318"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(235..1869)
                     /gene="lnt"
                     /locus_tag="PK049_11655"
     CDS             complement(235..1869)
                     /gene="lnt"
                     /locus_tag="PK049_11655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOK62257.1"
                     /translation="MQLKNLFKSNLFLMFLSGLLLALPWYEGLPSYTIFFGFVPLFVS
                     YYRLVEQKAGFWKIFGFVYLSFFTWNILATWWIYNATVIGAVLAVLITALLMTIVFSI
                     YHFIHINTSKTIGAFAFITFWIAWEQFFHNSEITWPWLTLGNSLGNHPMVIQWYEYTG
                     VLGGSLWILVVNFFILSQITAYRERVYGRIAWFFSDVLLFFTIPFALSLYIYFNHEEK
                     GEKVNVLIVQPNIDPYNEKFSGLTNEQQMDIMLNLAEENIDSTIRYVVCPETAIDDRI
                     WEHQLADNESVKRLKAFVDRHPGVMWITGLVSLKLYEPNEPVSHTARKLPYDERYYYD
                     RYNSAIQVDTLFNLPIYHKSKLVVGVEMMPYPQYLKFLEKLSIDLGGITGSLGTQPDR
                     GVFRSTDGKYGVGPIICYESVFGEYVAGYVQNGANLLFVITNDGWWGDTPGYRQHLSF
                     SRIRAIEMRRSIARSANTGISAIINQRGEVIDSLGWWKRGVIKGSVYANNATTYYARH
                     GDYIGNISAFMALLSLAYGISRFLMRRKKQESMSFS"
BASE COUNT          631 a          466 c          428 g          558 t
ORIGIN      
        1 tctaaagacg tgtgctaact agtgagttct taaatcttaa atcgagatcc ctcgattacg
       61 ctcgggatga caatggagtt ttaaatgttt tgctcctagg ggctggaagc gttgttcgtt
      121 gttcgtgcaa tcgttgttct tgaagctatt gccatgttta gctaagcgaa acgtctcatg
      181 ggatcgatta gagatccttc gctgtgctca ggatgacagg catgtatatt tttgttaact
      241 aaacgacatt gattcctgtt tcttccttcg catcaggaac cttgaaatac cataggctag
      301 tgagagtaaa gccatgaacg ccgagatatt tcctatgtaa tcgccatgac gggcatagta
      361 tgtagtagca ttattggcat atacagagcc ttttattact ccccgcttcc accaaccaag
      421 ggaatcaata acctcgccgc gctggtttat gatggccgat ataccggtat tggcggaacg
      481 ggctattgaa cggcgcatct caatggcgcg aatgcgcgag aacgagaggt gttgcctgta
      541 gcccggcgta tcgccccacc aaccatcgtt tgtgataaca aagagcaggt ttgctccatt
      601 ttgcacataa cctgccacat actccccaaa aaccgattcg tagcagatta ttggccctac
      661 gccatactta ccatcggttg aacggaaaac acctctatcg ggctgtgttc ctaaacttcc
      721 ggtaattccc cctaaatcga ttgaaagttt ctcaagaaac ttaaggtatt gcgggtaggg
      781 catcatctca accccaacaa ccagtttgga tttgtggtaa atgggcaaat tgaacaaagt
      841 atcgacctga atggctgagt tatacctatc gtagtaatac ctttcatcgt aaggcagctt
      901 tcgggcagta tgcgacactg gctcgttggg ctcataaagc ttaagcgata ccaaaccggt
      961 tatccacatc acaccgggat gcctatcaac aaaggctttc agccttttaa cactctcgtt
     1021 atccgctagc tgatgctccc aaatgcggtc gtcgatggcc gtctcaggac ataccacata
     1081 acgaatggta ctgtcaatgt tctcttcagc aagattaagc atgatgtcca tttgctgctc
     1141 gttagttaac ccgctgaact tttcattgta gggatcaatg ttaggttgaa ctatgagcac
     1201 attcactttt tcgcccttct cctcgtggtt aaagtagatg taaagcgaca gggcaaaagg
     1261 gattgtgaaa aagaggagaa catcggaaaa gaaccatgca atgcgaccat aaactctctc
     1321 gcggtaagca gtaatctgcg aaagaataaa aaagttaacc actaaaatcc aaagcgaacc
     1381 tcccaacaca cctgtatact cgtaccactg gatcaccata ggatgattac ccaggctatt
     1441 gcctagggtt agccatggcc aggttatttc ggaattatga aaaaattgct cccaggcaat
     1501 ccaaaaagtt ataaacgcaa aagcaccaat ggttttactc gtatttatat gaatgaaatg
     1561 gtaaatgctg aaaacaatgg tcattagcaa agccgtaatc aaaacagcca atacggctcc
     1621 aatcacagta gcgttataaa tccaccaggt agctaaaatg ttccaggtga aaaaagacag
     1681 gtacacaaat ccaaatatct tccaaaaacc agctttttgt tcaactagcc tatagtatga
     1741 tacaaaaagg ggaacaaacc caaaaaaaat agtgtagctt ggtaaaccct cataccatgg
     1801 gagcgctaaa agcagccccg aaagaaacat cagaaataaa ttggatttaa acagattctt
     1861 tagctgcatg gtggatgtta ttttaaaaac gatgtgtaaa gataaacgat ttgccgttct
     1921 tactaatcca attaaagcta tttttgggtg taatttaaaa tttgcttgct atggctctac
     1981 ctgaaataca cccatacatt ggaggtgaat tcatttccac aaacgaaacc taccatgttc
     2041 attgtccatg ggacaattct cccattgcca aggttcatct tgc
//
LOCUS       DAOSAL010000154         1959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_38581_length_1959_cov_54.859769, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000154 DAOSAL010000000
VERSION     DAOSAL010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38581_length_1959_cov_54.859769"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="PK049_11660"
     CDS             <1..483
                     /locus_tag="PK049_11660"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOK62258.1"
                     /translation="EFSSFAKLPTAKREPVNLIAVINDVITLYSSYKHIRIVLNNAAG
                     EPFVFADRDQLLRVFNNLVKNGIQAIERGKTGEVSISISKTSSSMVRVDVSDTGIGIP
                     KHAREKIFTPNFTTKSGGTGLGLAITREIILNFGGNIHFTSTENIGTTFTVDLPLYEA
                     "
     gene            complement(488..1771)
                     /locus_tag="PK049_11665"
     CDS             complement(488..1771)
                     /locus_tag="PK049_11665"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOK62259.1"
                     /translation="MKKGFGSFGKSFWTAITMEFFERGSYYGVMSILSVYLVLSTDQG
                     GLGFTKEKVGIIKSVITPLLYILPIVAGAIADRFGYRKTLFFAFAVMSSGYFLTSIST
                     SYSAVFLSLTLMAIGAGTFKPIISGTIARTTNESNSSLGFGIYYWSINLGAFLFPLLL
                     VPYLKSLGWNYILLMAAFGTGWLLLLNFFVFREPSRPQSQKNLVQVFADMLLVLKDYK
                     FILTIVIYSGFWILYFQMYDSVLWFLKERIDMTPVNNAVNSFLTIFVDNPSWRFDAEH
                     VTVVNAGTIILLQLIVSAILKNAKPLQTMIVGISFGTLGLGLLAISTYAWVFILGLVI
                     FTIGEMTAHPKFISYIGLIAPQDKKALYLGYSFLYGVIGSGIGGVLGAFAYVKFVTNM
                     NRPELFWLMFCGIGVLTIIGLILFNRFVLKGSKPS"
BASE COUNT          619 a          426 c          383 g          531 t
ORIGIN      
        1 gagttctcgt cgtttgcaaa acttcccacc gcaaaacgtg agcccgttaa ccttattgct
       61 gttataaacg atgttatcac cctttactcc agctataaac atatcaggat agtgcttaac
      121 aatgcagcgg gcgaaccgtt tgtttttgcc gacagggatc aacttttaag ggtgttcaac
      181 aaccttgtta aaaatggcat acaggccatt gagcgtggta aaaccggtga ggttagcata
      241 agtatatcaa aaacatccag cagtatggta agagttgatg tttccgatac gggcattggc
      301 ataccaaaac atgctcggga aaaaatcttt acgcctaatt tcacaaccaa atcgggagga
      361 accggtttag ggctagccat cacacgcgaa ataattttga atttcggagg taacatccat
      421 tttacctcta ccgaaaacat tggcacaacc tttacggtcg atctcccact ctatgaagct
      481 taactggtta gctcggcttt gaacccttta gaacgaacct gttaaacagg attaaaccga
      541 taatggtcaa tactccaatt ccgcaaaaca tcaaccagaa caactcgggg cggttcatgt
      601 tagttacaaa cttaacgtaa gcaaaagcac caagaacccc accaataccg ctacctatta
      661 ctccgtaaag gaatgagtag cccaggtaaa gcgctttttt atcctgtggc gcaattaaac
      721 ctatatagga aataaacttt gggtgggcgg tcatctcgcc aatggtaaaa atcactaatc
      781 ctaaaataaa tacccatgca taggttgaaa tggccaaaag gccaagtccc agtgtcccaa
      841 acgatattcc aactatcatg gtttgtaatg gtttggcatt ctttagaatt gccgatacta
      901 taagctgcaa aagtattatg gttcccgcat taactaccgt aacatgttca gcatcaaacc
      961 tccacgatgg attatcgaca aaaatggtca aaaaagaatt tacagcattg ttaaccgggg
     1021 tcatgtcgat acgctccttt aagaaccaga gtacagagtc gtacatctga aagtaaagaa
     1081 tccagaaacc tgagtatatt acaatggtta gtatgaactt ataatctttt agaaccaaaa
     1141 gcatatcggc aaaaacctga acaaggtttt tctggctttg tggccttgag ggttcacgga
     1201 acacaaagaa atttaataat agcagccaac ctgtcccaaa tgcggccatc agcagaatgt
     1261 agttccaacc caacgattta aggtaaggga ctaagagtaa tgggaaaaga aaggccccaa
     1321 ggtttataga ccagtaatat attccaaatc ctaacgatga gttcgactcg ttggtggttc
     1381 gggctattgt tccggaaatt attggtttaa aggttcccgc acctattgcc atgagcgtaa
     1441 gacttaggaa tacagcagag tatgaggtgc taatgcttgt taggaagtac cctgaactca
     1501 ttacggcaaa ggcaaagaat agtgtttttc ggtagccaaa tctatcggct atggcccctg
     1561 ccactatggg gagtatatat agtaacggcg taataacgct ttttatgatt cctacctttt
     1621 ccttggtaaa acccaatccg ccttgatcag tactaagaac caggtaaacc gaaagaatcg
     1681 acatcacacc gtagtatgag cccctttcaa agaattccat ggtaatggca gtccaaaaac
     1741 ttttaccaaa cgagccaaag ccttttttca tagttactca caattaattt tcgcaatatt
     1801 caactttttt cccatttgta agcatgataa aaatcaaatt gtaaaaaaag ggggatatta
     1861 tccccccagc ctctatattg caagtaagtt ttactttgtt ttgcttgcct tttcgtaagc
     1921 cttttgggcc tccttgaggg catcctccgc aagtttctt
//
LOCUS       DAOSAL010000155         1671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_48602_length_1671_cov_45.863861, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000155 DAOSAL010000000
VERSION     DAOSAL010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48602_length_1671_cov_45.863861"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="PK049_11670"
     CDS             <1..288
                     /locus_tag="PK049_11670"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOK62260.1"
                     /translation="NKGNITIGCEYFDSSLLFKVSDTGIGVPRDKQKVIFDRFVRANN
                     DLTNPVDGAGLGLSIAKAYVERLGGKIWLESEPGRGSTFYFTIPLSPNVNF"
     gene            complement(568..>1671)
                     /locus_tag="PK049_11675"
     CDS             complement(568..>1671)
                     /locus_tag="PK049_11675"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOK62261.1"
                     /translation="SVLILTMEAFKNHIVTTERRDALLKVRVINLLNMEQHYIDFGEE
                     VYTALPSVNPEFNTNLFYYYYTSFTTPGSTCEYNMTTRKKRLLKQNEVLGGFDPTKYE
                     SKRIWATAPDSTTIPISLVYRKGTRFSANTPLLLYGYGAYGYSINTTFDPNLISLLDR
                     GVVYAVAHVRGGQEMGRHWYNQGKLLNKKNSFTDFVACAEKLIADGYTSASKLIAHGS
                     SAGGLLVGAALNMRPDLFKAVVAEVPFVDVLTSMLDSTQALTTVEYNEWGNPHDSTFY
                     YYIKSYSPYDQIKKMPYPNMLVTSGYNDNQVQYFEPVKWVAKLRANNTGNSQILLWID
                     MSLGHSQSSGRYLQLKDQAFIYAYMLNQWGITE"
BASE COUNT          461 a          348 c          350 g          512 t
ORIGIN      
        1 aataagggaa acataaccat tggttgtgag tattttgata gttcactact atttaaggta
       61 agcgatacag gtataggagt accacgggat aaacaaaaag tgatttttga tcgctttgtg
      121 cgagccaata atgatttgac taatccggtt gatggtgctg gtttgggatt atcaatagca
      181 aaggcttatg ttgaaagact tggtggtaaa atttggttgg aaagcgaacc cggtagaggc
      241 tcaaccttct acttcactat tcccttaagt ccaaatgtta acttctaatt catcaactga
      301 ctaaaattcg tcaactgttt ttttcgataa acagctcccc tctttaaatg tttccgcctt
      361 ccattttgat tatctcagct gaatttatta atccatagca attcggatta actgttcact
      421 ttttgctatt cctctaactt cctctaactc cctcatattc cgactcattc cgattcattc
      481 cgtctcattc ctcaagaaat aaatccgttt gaattttaca atttatggca cacgggttaa
      541 atagccgtct ccctttgcca gaaatactta ttcggttata ccccactggt ttaacatgta
      601 ggcatatata aaagcctgat ccttgagttg aaggtaccta cccgatgatt ggctatggcc
      661 aagcgacata tcaatccaaa gcaaaatttg gctgttaccg gtattattag cccgtagctt
      721 agccacccac tttacgggct caaagtattg cacttgattg tcgttgtaac ccgatgtaac
      781 aagcatgtta gggtagggca tctttttaat ttggtcgtag ggcgagtacg attttatgta
      841 gtagtagaag gtggaatcgt gaggatttcc ccactcgttg tattcaacag tagttaatgc
      901 ttgtgttgaa tcgagcatcg atgtaagcac atctacaaaa ggcacctctg caaccacagc
      961 tttgaataga tcggggcgca tgtttaatgc tgctcccaca agtaatccgc cagcactgct
     1021 accatgagct ataagttttg aagctgaggt atagccatcg gcaataagtt tctcggcgca
     1081 agcaacaaaa tcggtaaaag agtttttctt gttcaggagt ttgccttggt tgtaccagtg
     1141 ccgacccatc tcctgcccac ctcgcacatg ggcaactgca tatactaccc ccctgtcaag
     1201 taagctaatg agatttggat cgaaggtggt gttgatggag taaccgtaag caccataacc
     1261 gtaaaggagc aagggtgtat ttgcgctaaa cctggtacct ttcctatata ccagtgatat
     1321 tggaatcgta gtactgtccg gggcagttgc ccaaatccgc tttgactcat atttagtagg
     1381 atcaaaacca cccagaactt cattttgctt gagcagcctt tttttacggg tagtcatgtt
     1441 gtattcgcaa gtggaaccgg gagtggtaaa cgaggtgtag tagtaataaa aaagattggt
     1501 gttaaactca gggtttactg aaggaagagc agtataaacc tcctcgccaa aatcgatata
     1561 atgctgctcc atattcagta ggtttattac ccttactttc aatagcgcat cgcggcgttc
     1621 agttgttact atgtggtttt taaaggcttc catggtaaga attagtaccg a
//
LOCUS       DAOSAL010000156         1531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Tenuifilum sp. isolate AMR_MDS_1910
            NODE_55246_length_1531_cov_54.739837, whole genome shotgun
            sequence.
ACCESSION   DAOSAL010000156 DAOSAL010000000
VERSION     DAOSAL010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863439
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Tenuifilum sp. (wastewater metagenome)
  ORGANISM  Tenuifilum sp.
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tenuifilaceae;
            Tenuifilum.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:11:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,338
            CDSs (total)                      :: 2,299
            Genes (coding)                    :: 2,295
            CDSs (with protein)               :: 2,295
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Tenuifilum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55246_length_1531_cov_54.739837"
                     /isolate="AMR_MDS_1910"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2760880"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="PK049_11680"
     CDS             <1..363
                     /locus_tag="PK049_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK62262.1"
                     /translation="FLVNNNRELYEAKRRAYDNESLADLATKTFEKNKIVQYGDELVQ
                     QYDPVYRDPIPRHYLDFRSHFLAPRKHFLGMYFDTFWFNLVIIWLMTIALYITLYYES
                     LKKLLDFLGKIKIPTLKK"
     gene            401..1327
                     /locus_tag="PK049_11685"
     CDS             401..1327
                     /locus_tag="PK049_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK62263.1"
                     /translation="MKKLRFIYYLSLFAILSQVITACNSCSKQGVDVTQDLYVPDSVI
                     EVAPPQVSKAVTEQMMQNISSPVETAALIKGLKIPFNKNFLASTKQAEKFNTNFEKAF
                     ALGLYGCDLGYLNMYEKTSFVVDYISTIKRLADDIQVGQFFDFNTLKRLATNNENLDS
                     LILISQQSFNRIDNYLAETNRSMLSAVIVAGVWVEGAYLSSRVAQISNHPRIRETIGE
                     QKTILNFLLALLKVYDKDPSMAKLITDFENIKKIYDEVKITYEVGEPEMVEENGVLTF
                     KQTDKQIIQMDDELYQRLIVGIQELRNNLLKL"
     gene            1341..>1531
                     /locus_tag="PK049_11690"
     CDS             1341..>1531
                     /locus_tag="PK049_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOK62264.1"
                     /translation="MKKILFLLLVVLMGVTYFNKVNAQSEEELVQICTMVAGDAEYLK
                     DFRIKLDAGDPPPVQRFPIL"
BASE COUNT          478 a          319 c          304 g          430 t
ORIGIN      
        1 ttccttgtaa ataataaccg ggaactctac gaggccaagc gtcgtgccta cgataacgag
       61 agccttgccg acctggcaac aaaaaccttt gaaaagaaca agattgtgca atacggcgat
      121 gagctggttc agcagtacga tcccgtttac cgcgacccaa tcccaagaca ttacctcgat
      181 tttcgttcac atttcttagc tccccgcaag cattttcttg gcatgtattt tgatactttc
      241 tggtttaacc tggtaatcat ttggcttatg accatagcgc tatacataac cctttactac
      301 gagtcgctaa aaaaattgct cgattttttg gggaaaatta aaattccaac tctaaaaaaa
      361 taaaattttg tatctttact tttcgaaaca ggatattaaa atgaaaaaac tgcgttttat
      421 atactaccta tcgctgtttg caatactttc acaagttatt accgcttgca actcgtgtag
      481 caaacaaggg gttgatgtga cacaggattt atatgttcct gactcagtga ttgaggttgc
      541 accgccccag gtaagcaaag cggttactga gcaaatgatg caaaatattt cgtcgccagt
      601 tgaaacagca gcccttatta aagggttaaa aatacctttt aataagaact ttctggccag
      661 caccaagcaa gccgagaagt tcaacacaaa ctttgagaaa gcatttgccc ttgggcttta
      721 tggttgcgat ttgggttacc tcaacatgta tgagaaaacc tcgtttgtgg tcgattacat
      781 ctcaaccatt aagcgtttag ccgatgatat tcaggtaggt caattctttg actttaatac
      841 cctgaagcga ctggccacca ataatgaaaa cctggactca ctaatcctta tctcacagca
      901 aagcttcaac cgtattgata actacttggc tgaaacaaac cgctcaatgc ttagcgctgt
      961 aattgtagcc ggtgtttggg ttgagggggc ttacctttcg agccgcgtgg ctcaaattag
     1021 caatcatccc aggattaggg aaaccattgg agaacaaaaa accattctta acttcctact
     1081 tgccctccta aaggtttacg ataaagatcc cagcatggca aagcttataa ccgattttga
     1141 aaacatcaaa aagatatacg atgaggtaaa aattacttac gaagtaggag aacctgaaat
     1201 ggtggaggaa aacggagttt taaccttcaa gcaaaccgat aagcaaataa ttcagatgga
     1261 cgatgaactc taccaaaggt tgattgtggg tatacaggaa ttacgtaaca atttattaaa
     1321 actgtaagaa attttcagct atgaaaaaga tattattctt gttattggtg gtgttgatgg
     1381 gtgttaccta ttttaacaaa gtaaatgccc aatcggaaga ggagttggtg caaatctgca
     1441 ccatggtagc cggcgatgcc gaatacctaa aggatttccg cattaagctc gatgctggtg
     1501 accctccccc tgtacaacga tttccaattc t
//
